LOCUS       DASRVO010000001         1013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_285805, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000001 DASRVO010000000
VERSION     DASRVO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285805"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFF05_00005"
     CDS             <1..684
                     /locus_tag="VFF05_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HZW50216.1"
                     /translation="AINATLYKNLPFDTLKDFSPVILGATSPNILIVNPQVPAKTVAE
                     LIAYAKANPGKLTVGSSGVGTAPQMTAVLFRERAGIKVVDVPFKGGPEATAALLGGQI
                     DYLFAIASTALPHIKAGRVRALGITSRHRSPLLPDVPTIAESGLPGFEAVTWFGFEVP
                     RGTPRAIIDRLNKEIGNALATPEVKDKLAVQGLYVEGGTPERFGNYMRDEFAKWGGLV
                     KETGMSLKE"
BASE COUNT          192 a          317 c          328 g          176 t
ORIGIN      
        1 gcgatcaacg cgacactgta caagaacctg ccgttcgaca cgctgaagga tttctcgccg
       61 gtgatcctgg gcgccacctc gcccaacatc ctgatcgtca atccgcaggt cccggcgaaa
      121 acggttgccg agctcatcgc ctacgccaag gccaatcccg gcaagctcac ggtcggctcg
      181 agcggcgtcg gcaccgcgcc gcagatgacc gcggtgctgt tccgcgagcg cgctggcatc
      241 aaggtcgtcg acgtgccttt caagggcgga ccggaagcga ccgcggcgct gctcggcggg
      301 cagatcgatt acctgttcgc catcgcctcg accgcgttgc cgcacatcaa ggccggccgc
      361 gtgcgcgcgc tcggcatcac gagccggcac cgctcgccgt tgctgcccga cgtgccgacc
      421 atcgccgaaa gcggcttgcc cggcttcgag gcggtgacct ggttcggctt cgaagtgccc
      481 aggggcacgc cgcgcgccat catcgaccgg ctgaacaagg agatcggcaa cgcgctggcg
      541 acgcccgagg tcaaggacaa gcttgccgtg cagggcctct atgtcgaagg cggcacgccg
      601 gagcgattcg gcaattacat gcgcgacgaa ttcgccaaat ggggcggcct ggtcaaggaa
      661 accgggatgt cactcaagga atgaggcctg accggccgcg gctgaaatgc ccacggccgg
      721 ttagtgcaaa tcgaacagtt ttaaataata gtctaaacga tccttgtaaa tcttgcgcga
      781 tagtttcgtt cgaggttgcc gccgcgcttc gcgcggctgg tcgatcgagc ggaggctatc
      841 gtgctcgaat caatgaatgc ctggatcgag tccatgcggt tcgcgtgcga ggtacagggc
      901 gtcatctcga tgcgtctgat gttcctgtcg cagggcgggc ccgaagccga gatggaagcg
      961 cgcctgatgg tatcggaaaa aatggcggcc tttgccgacg ccgaagtggc gct
//
LOCUS       DASRVO010000002         3340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_311793, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000002 DASRVO010000000
VERSION     DASRVO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311793"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /gene="dnaE"
                     /locus_tag="VFF05_00010"
     CDS             complement(<1..1935)
                     /gene="dnaE"
                     /locus_tag="VFF05_00010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZW50217.1"
                     /translation="MNPSFVHLHVHSEYSLVDSTLRMPDKPEYGDPAKAKRPNLISRA
                     VQTGFPALALTDESNLFALVKFYRAAEANGVKPIAGADLWIVDPADGATPTRLTVLCQ
                     NHDGYLHLSQLISRSYTEGRRGEHALVDADWFAGHSAGLIALAGRESDIGRALIAGKP
                     DFALAETQKWLRHFDDRLYLELTRCGRADEAHFVPAALQLAVDADCPVVATNDVRFAV
                     REDFEAHEARVCIHQGRLLNDPKRPRDYTPEQYLKSAEEMAELFADIPEALENSVEIA
                     RRCNLEMKFGEYFLPAFPVPTQASEAAFIRTQSQQGLQARLSKHGFAPEFAHEDYTSR
                     LERELDVIVKMGFAGYFLIVADFIGWARQHDIPVGPGRGSGAGSVVAWSLGITDLDPL
                     RYGLLFERFLNPERVSMPDFDVDFCMDRRDEVIDYVAHKYGREKVSQIITYGTMAAKA
                     VLRDAGRVLGMGYGQVDQIAKLIPLKPTDPLSLDDALGASERSRRETDRIVPEFKSRY
                     ESEDEVRELVDLARSIEDLVRNAGKHAGGVVIAPSALTDFAPLYSEGGGDGVVTQFDK
                     DDVEAVGLVKFDFLGLRTLTIIDWAVKAINARRLATGEAPLDIAALPLDDACVYALFQ
                     NARTVAVFQFEGNGMQRMLVK"
     gene            complement(2001..2792)
                     /locus_tag="VFF05_00015"
     CDS             complement(2001..2792)
                     /locus_tag="VFF05_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZW50218.1"
                     /translation="MELNLAGKHALVCGGSQGIGRATAIELAQLGADVTVLARSREAL
                     QAVVDSLPKTRPAQTHSSIATDADDTSALGAAIETLARAWPVQILINNSGGPPGGPAH
                     SAKVEEYTDAFRRHLIANQTALQAVLPGMRAAGYGRVVNIVSTSVKEPIDNLGVSNTV
                     RAAVAGWAKTCSRELAPFGITVNNVLPGFTKTQRLTSLIAAKAKASGKSEDAIAAEML
                     ATVPAARFAEPVELAAVVAFLCTPAAAYVNGVSIAVDGGRTHALS"
     gene            complement(2792..3100)
                     /locus_tag="VFF05_00020"
     CDS             complement(2792..3100)
                     /locus_tag="VFF05_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50219.1"
                     /translation="MTADDEYWWIAALGDMLVWARLRVRDSGNAEVFDARGETLVYDD
                     EDSARAALLDAEFRAFDGLDEDDAALLGFDLDSFAPPEAEDDEELLPLMTEKLARGHA
                     "
     gene            complement(3103..>3340)
                     /locus_tag="VFF05_00025"
     CDS             complement(3103..>3340)
                     /locus_tag="VFF05_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZW50220.1"
                     /translation="EIIGGSQREERLDVLDARMRQFKLDPAHYQWYRDFRRYGSVPHA
                     GFGLGFERLVVYVCGLSNIRDAIPYPRVPGSADF"
BASE COUNT          590 a         1026 c         1112 g          612 t
ORIGIN      
        1 cttcaccagc atgcgctgca tgccgttgcc ttcgaactgg aataccgcca ccgtgcgcgc
       61 gttctggaac agcgcgtaga cgcacgcatc gtcgagcggc aatgcggcga tgtcgagtgg
      121 agcctcgcca gtcgccaagc ggcgggcgtt tatcgccttc accgcccagt cgatgatggt
      181 cagcgtgcgc aggccgagga agtcgaactt gaccagcccc acggcctcga cgtcgtcctt
      241 gtcgaactgg gtgacgacgc cgtcgccgcc gccttccgaa tacagcgggg cgaagtcggt
      301 cagcgcgctc ggtgcgatca ccacgccgcc ggcgtgcttg ccggcattgc gcaccaggtc
      361 ttcgatgctg cgcgcgagat cgaccagttc acgtacctcg tcttcggatt cgtagcgcga
      421 tttgaattcc gggacgatgc ggtcggtttc cctgcgtgag cgttcgctgg cgccgagcgc
      481 gtcgtcgagg ctgagcggat cggtcggctt gagcggaatg agcttggcaa tctggtcgac
      541 ctgaccgtag cccatgccga gcacgcggcc ggcatcgcgc agcacggcct tcgccgccat
      601 cgtgccgtag gtgatgatct ggctgacctt ctcgcggccg tacttgtgcg cgacatagtc
      661 gatcacctcg tcgcggcgat ccatgcagaa gtcgacgtcg aagtcgggca tggacacgcg
      721 ttcgggattg aggaatcgct cgaacagcaa tccatagcgc agcggatcga ggtcagtgat
      781 tccgagcgac caggcgacga cggagcctgc accggaaccg cggccggggc cgaccggaat
      841 atcgtgctgt ctggcccagc cgatgaagtc ggcgacgatc agaaaatacc cggcgaagcc
      901 catcttcacg atcacgtcga gttcgcgttc gaggcggctc gtgtaatcct cgtgcgcgaa
      961 ttcgggagcg aatccgtgct tggacaggcg cgcctgcaga ccctgttgcg attgggtacg
     1021 gatgaacgcg gcttccgaag cctgcgtcgg aaccggaaac gcgggcagga aatattcgcc
     1081 gaacttcatt tccaggttgc agcgcctggc gatctccacg ctgttttcca gcgcttcggg
     1141 aatgtcggcg aacaactccg ccatctcctc ggcggatttc aggtactgct cgggcgtgta
     1201 gtcgcgtggg cgcttgggat cgttgagcag gcgcccctgg tggatgcaca cgcgcgcctc
     1261 gtgcgcctcg aaatcctcgc gtacggcgaa gcgcacgtcg ttcgtcgcca cgaccgggca
     1321 atcggcgtct acggcgagtt gcagcgcggc gggaacgaaa tgcgcctcgt cggcgcggcc
     1381 gcaacgggtc aattccagat acagacgatc gtcgaagtgg cgcagccatt tttgcgtttc
     1441 ggcaagcgcg aaatcgggtt tgccggcgat gagggcacgg ccgatatcgc tttcgcggcc
     1501 ggccaaggcg atgagcccgg cgctgtgtcc cgcgaaccag tccgcatcga ccagggcgtg
     1561 ttcgccgcga cgaccttcag tgtaactgcg cgagatgagc tgcgacaaat gcaggtagcc
     1621 atcgtggttc tggcacagca cggtcagacg cgtcggtgtc gcgccatcgg cgggatcgac
     1681 gatccacaag tcggcgccgg cgatgggttt gacgccgttc gcctcggcgg cgcgatagaa
     1741 cttcaccagc gcgaacaagt tgctttcgtc ggtcagcgcg agcgcgggaa aacccgtctg
     1801 cacggcacgg ctgatcagat tcggacgctt cgccttggcc ggatcgccgt attcgggctt
     1861 gtccggcatg cgcagggtcg aatcgaccag cgagtattcg ctgtggacgt gaaggtgtac
     1921 gaaggaggga ttcatggcga ccgcaggcta cctttgcagg ttagcccgcg gctgcggtgc
     1981 gggcaagact tgacgcgagg tcaactcaac gcatgcgtgc gcccgccatc gaccgcgatg
     2041 ctcacgccgt tgacgtaggc agccgcaggc gtgcacagga aggcgaccac ggcggccaat
     2101 tcgacgggtt cggcaaagcg tgcggcggga actgtggcaa gcatttctgc ggcaatcgcg
     2161 tcttcggatt tgccggaagc cttggccttg gcagcgatca ggctggtcag gcgctgcgtc
     2221 ttggtgaaac ccggcagcac gttgttgacg gtgatgccga acggcgcgag ttcgcgcgag
     2281 caggtcttgg cccagccggc aacggcggcg cgcacggtat tggagacgcc gaggttgtcg
     2341 atcggttcct tgaccgaggt ggaaacgatg ttgaccacgc ggccataacc cgcggcgcgc
     2401 atgccgggca gcaccgcctg cagtgcggtc tggttggcga tgagatgccg gcgaaaagcg
     2461 tccgtatact cttccacttt tgcggaatgc gccggtccgc ccggtgggcc gccactgttg
     2521 ttgatcaaga tctgtaccgg ccaggcacgc gcaagggttt cgatggcggc gccaagtgcg
     2581 gacgtgtcgt ccgcgtccgt cgcaatgctg gaatgggttt gtgccggacg cgttttcggc
     2641 aaagaatcca cgacggcttg caacgcttcg cgcgatcgcg ccagaaccgt gacatccgcg
     2701 ccgagttgcg ccagttcgat ggcggtggcg cgaccgatgc cctgcgagcc gccgcagacg
     2761 agcgcatgtt tgccggcaag attcagctcc atcaggcatg tcctcgggcg agtttttccg
     2821 tcatcagcgg caataattcc tcgtcgtctt cggcttccgg cggtgcgaag gaatccagat
     2881 cgaaacccag cagcgcggcg tcgtcttcgt ccagcccgtc gaaggcgcgg aattccgcgt
     2941 cgagcagtgc ggcgcgcgcg ctatcctcgt cgtcatagac cagcgtttcg ccgcgcgcgt
     3001 cgaacacttc ggcgttgccc gaatcgcgca cgcgcaggcg cgcccacacg agcatgtcgc
     3061 cgagtgcggc aatccaccag tattcatcgt ctgcggtcat gatcagaagt cagcgctgcc
     3121 cggcacgcgc gggtagggga tcgcgtcgcg gatattggaa agaccgcaga cgtatacgac
     3181 aagacgttcg aagccgagtc cgaatcccgc atgcggaacg ctgccgtagc ggcggaaatc
     3241 gcgataccac tggtaatgtg ccggatcgag cttgaactga cgcatgcgtg catcgagcac
     3301 atcgaggcgt tcctcgcgct gcgagccgcc gatgatctcg
//
LOCUS       DASRVO010000003         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_93, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000003 DASRVO010000000
VERSION     DASRVO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VFF05_00030"
     CDS             complement(<1..153)
                     /locus_tag="VFF05_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZW50221.1"
                     /translation="MPLTWPTILTLFRIALLPVMVAVFYAGFHGANVAAAIIFLGAAI
                     TDWLDGY"
     gene            complement(179..2055)
                     /gene="uvrC"
                     /locus_tag="VFF05_00035"
                     /pseudo
     CDS             complement(179..2055)
                     /gene="uvrC"
                     /locus_tag="VFF05_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462397.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
     gene            complement(2060..>2665)
                     /locus_tag="VFF05_00040"
     CDS             complement(2060..>2665)
                     /locus_tag="VFF05_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50222.1"
                     /translation="LEPAKVAMSAPADVAVLGDNVYIAVSGQNQIWQLDMVRNKLVVL
                     AGSGQLGLTDGVGVTASFAQPVSLSVIGQQLAIADAAASAVRLLRLLDNRVSTLAGTG
                     LYDFGDAAGKREDARLQHPLAVCADARGLIFVADSYNGKIKAINMRTGDVRALNLPYR
                     LQEPAGLALGAGALWVANTNAHEIVRVDLASGQIRRLTISE"
BASE COUNT          460 a          882 c          856 g          467 t
ORIGIN      
        1 gtaaccgtcc agccagtcgg tgattgcggc gccgagaaag atgatcgctg cggcgacgtt
       61 ggcgccatgg aatcccgcat agaacaccgc caccatcact ggcaacaacg cgatgcggaa
      121 cagggtgagg atggttggcc aggtcagcgg catgccggcg agtgtgccac gtctgcgctc
      181 agccgtggaa agcggcgtag attttcgcgg ccagttcgcg gcttacgcca ttgacttggg
      241 cgagctcgtc gatgcccgcc gccgccacac cgccgatgcc accgaaatgc ttgagcagcg
      301 ccgagcgccg cttcgcgccg acgccggcga tttcctccag ccggcttttt tcgcgtgcct
      361 tctcgcggcg ctggcgatgt ccggtgatgg cgaagcgatg cgcctcgtcg cggatggcgt
      421 cgagcagatg caaggcaggc gattccgggc ccggccacag cgtcttgccg ttgtcgccgc
      481 ggatcaggct ttcgtggcca gagcgccgct cgggcccctt ggcaatgcca agcaccaaca
      541 cgccgctcac acccaggtcg gcgagcactt ccagcgcctg tgcgacctgg cccttgccac
      601 cgtcgatcag caggatgtcc ggcaacacgc cctcgcccgc ctgcaggcgc ttgtaacggc
      661 gctccagggc ctggcgcatg gccgcgtagt cgtcgcccgg ctcgatgccg gaaatgttga
      721 agcgccgata cgccgatttg actgcgccct cgggaccgta gatcacgcag gaagccaccg
      781 tcgcctcgcc catcgtgtgc gaaatgtcga agcactccag ccgttgcggc gtatcgccga
      841 gttcgagcag ttcgcgcagc gcttcgaacc gctcgtgtat ggtttgctga ctggccaggc
      901 gcgcggccag cgccgcctgg gcgttcttgc gcgccagttc cacgaaatgc gcacggtcgc
      961 cgcgcacctg cgatttgacc tccaccatgt gcccggcctg ttcggacagc gcagcggcaa
     1021 tcaagtccgc ctcgtcgggc gcatcgctga acagcagctc gcgcggcacg ggtttgtcga
     1081 gataatactg cgcggcaaag gcggcgagca ccgcgccctt atccgcatcc gcgggcaggc
     1141 gtgggaagaa gtcgcgcgag cccaggttca cgccattgcg gaaaaacagc acggaaacgc
     1201 agaccgtgcc ggactcgatg cggcaggcga gcgcatcgag gtcggcgctg gcgccctgca
     1261 cgtgcacctg tgcgtgcaaa cgcttgaggg tggcgatctg gtcgcgcagg cgcgcggcct
     1321 tttcgaattc cagcaccgcg ctggcctgct ccatcgtcac gccaagttcc tcgatcaccg
     1381 cactgctctt gccgtgtagg aacattgccg catggcgcac ggattcacgg tactcatcca
     1441 cgccgatgaa acccacgcag ggcgcggtgc aacgcccgat ctggtattgc aggcagggcc
     1501 gcgaacgatt gcggaagtag ctgtcctcgc attggcgcac gcggaaaagc ttctgcatca
     1561 aatcgagact ttcgcgcacc gcccatgcgc tcggatacgg tccgaaatac tcgccctggc
     1621 cggtcttcgc gccgcggtgg aacgacagtc gcgcgaagtc cgccggatcc ggccgctcgt
     1681 tggcacgcct tgcctgctgc gcagctgggc ttgcggacag atggatgtgg gggtaactct
     1741 tgtcgtcgcg cagcaggatg ttgtagcgcg gcttaggctc ttgatgagct gagcttcgag
     1801 cagcagcgct tcgccttcgg tgcgcgtaag ggtgacttcg atgcgcgcga tctgcgcaat
     1861 catgctggcg atgcgcggct gcaacgcggg cttcaggaaa taactgccga cgcgcttttt
     1921 cagattgcca gccttgccga cgtacaacgg ttcgccgcgc gcgtcgagca tgcgataaac
     1981 gccgggcgaa gtggtcaggg tgcgtacgaa ttgcttgcca tcaaaactcg acgcactgga
     2041 atccgactcg ctcacgcgtt cactcggaaa tggtcaggcg ccggatctgt ccactggcaa
     2101 gatccacgcg cacgatctcg tgcgcgttgg tattggcgac ccacaatgcg ccggcgccga
     2161 gcgcaagacc agccggttcc tgcaaccggt acggcagatt cagggcgcgc acgtcgcccg
     2221 tgcgcatgtt gatcgccttg atctttccgt tgtagctgtc ggccacgaag atcagaccgc
     2281 gggcatccgc gcaaaccgca agaggatgct gcaggcgcgc atcctcgcgc ttgcccgcag
     2341 catcgccgaa atcgtatagg ccggtacccg ccaaggtgct gacacgattg tcgagcaggc
     2401 gcagcaggcg caccgccgaa gcggccgcgt cggcgatcgc gagctgctgc ccgatcacgc
     2461 tcaggcttac aggctgggcg aaactcgcgg tcacgccgac gccgtcggtc aagccgagtt
     2521 ggccgctccc ggccagcacc acgagcttgt tgcgcaccat gtcgagttgc catatctggt
     2581 tctggccgga caccgcgatg tagacgttgt cgccgagcac ggcgacatcg gccggcgccg
     2641 acatcgccac tttcgccggt tccag
//
LOCUS       DASRVO010000004         1123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_207971, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000004 DASRVO010000000
VERSION     DASRVO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207971"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFF05_00045"
     CDS             <1..231
                     /locus_tag="VFF05_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZW50223.1"
                     /translation="GWMYRVLTRIVEGKASMEDVQMLKQAAGQIEGHTICAFGEAAAW
                     PVQGMLRHFWHEFEYYIANKRSIVDAPIGVAA"
     gene            228..>1123
                     /locus_tag="VFF05_00050"
     CDS             228..>1123
                     /locus_tag="VFF05_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZW50224.1"
                     /translation="MSAVPVNPNTPPDMVNIEIDGKPLKVPKNSMIIQAADAAGIPIP
                     RFCYHKKLPIAANCRMCMVETEMNGKASPKPAPACATPVMEGMKVNTQSNRALSGQRN
                     AMEFLLINHPLDCPICDQGGECELQDLSLGYGRSVSRFAERKRVVPDEDLGPLISTEM
                     TRCIHCTRCIRVMTEVAGSPELGGMGRGEHLEIGTYIGKTIDSELGGNIIDVCPVGAL
                     TNKVFRFKARAWELIAREGIGYHDALGSNLWLHTRRGEVLRTVPRDNESINECWLSDR
                     DRYSHQGLYAADRVGAPEVKRNG"
BASE COUNT          231 a          363 c          350 g          179 t
ORIGIN      
        1 ggctggatgt accgcgtgct cacgcgcatc gtcgaaggca aggcaagcat ggaagacgtg
       61 cagatgctca agcaggccgc cggccagatc gaaggccaca ccatctgcgc gttcggcgaa
      121 gccgcggcgt ggccggtgca gggcatgctg cggcatttct ggcacgagtt cgagtactac
      181 attgcgaaca agcgctcgat cgtcgatgcg ccaatcggag tggccgcatg agcgccgttc
      241 ccgtcaatcc gaataccccg cccgacatgg tcaacatcga aatcgacggc aagccgctca
      301 aggtgccgaa gaactcgatg atcatccagg ctgcggatgc agccgggatt ccgatcccgc
      361 gtttctgcta ccacaagaaa ttgccgatcg cggcgaactg ccgcatgtgc atggtcgaga
      421 ccgaaatgaa cggcaaggcc tcgcccaaac ctgcgccggc ctgtgccacg ccggtgatgg
      481 aaggcatgaa ggtgaacacg caatcgaacc gcgcgctgtc cggccagcgc aacgcgatgg
      541 aattcctgct catcaaccat ccgctggatt gcccgatctg cgaccagggc ggtgaatgcg
      601 aattgcagga cctgtcgctc ggctatggcc gctcggtgtc gcgcttcgcc gaacgcaagc
      661 gcgtggtgcc ggacgaagac ctcggcccgc tgatctccac cgagatgacg cgctgcattc
      721 attgcacgcg ctgcatccgc gtcatgaccg aagtcgccgg ttcgccggaa ctcggcggca
      781 tgggccgcgg cgagcacctg gaaatcggca cgtatatcgg caagaccata gactccgaac
      841 tcggcggcaa catcatcgac gtgtgcccgg tcggtgcgtt gaccaacaag gtgttccgct
      901 tcaaggctcg cgcctgggaa ctgatcgcgc gcgaaggcat cggctaccac gatgcgctcg
      961 gttccaacct gtggctgcac acccgacgcg gcgaagtgct gcgcacggtg ccgcgcgaca
     1021 acgaatcgat caacgaatgc tggctgtccg atcgcgaccg ctacagccac cagggcctgt
     1081 atgccgccga ccgcgtcggc gcacccgaag tgaagcgcaa cgg
//
LOCUS       DASRVO010000005         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_337870, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000005 DASRVO010000000
VERSION     DASRVO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337870"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFF05_00055"
     CDS             <1..252
                     /locus_tag="VFF05_00055"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HZW50225.1"
                     /translation="REAIAGMLARGDAATRWLHAGTGALAELRRHPAWIAGGVGLLFA
                     LRPKRVLKWLASGWSLWRVYRGARAWLWRAIPREDIRSR"
     gene            complement(227..1000)
                     /locus_tag="VFF05_00060"
     CDS             complement(227..1000)
                     /locus_tag="VFF05_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZW50226.1"
                     /translation="MRVITLNVNGIRSAAGKGLFRWLSAQRADVICLQEVKAQESDLD
                     AALHAPRGFAASYALAEKKGYSGVALYSRRAPDAVSRGFGTHEFDSEGRYLEARFGRL
                     SVISLYLPSGSSGPHRQASKFRFLEAFLPQLKKLARARREVILCGDWNIAHKEIDLRN
                     WRSNQKNSGFLPEERAWLSRVFDEIGLVDVFRRLNHKPDQYTWWSNRGQAWAKNVGWR
                     IDYQIATPAVAKTAVRESIYKRRRFSDHAPLIIDYEYPL"
     gene            complement(1001..1690)
                     /locus_tag="VFF05_00065"
     CDS             complement(1001..1690)
                     /locus_tag="VFF05_00065"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZW50227.1"
                     /translation="MHSADRSFIAETTAKMLLEVKAVLFSDDKPFTFTSGWASPVYID
                     CRKLIFYPRIRSQLVDFAAATLQRDAGFEQFDVVAGGETAGIPYAAWIADRLGLPMQY
                     VRKKPKGFGRNAQIEGDVRDGARTLLVEDLTTDGRSKINFCQALRNAGAVVDHVFVNF
                     YYDIFPESKKILADLNVRLHHLATWWDVLAVVKRGAYLPAAQIAEVEKFLNAPAEWSA
                     AHGGAASSQSS"
     gene            1799..2383
                     /locus_tag="VFF05_00070"
     CDS             1799..2383
                     /locus_tag="VFF05_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50228.1"
                     /translation="MKLFAAALTMLAFAAIPLAAQAQAFRCVGKDGKKYYGQIIPQAC
                     IGQPYEQLNMQGMVIKRFDPTVDNVDPATKAAEEEKQREKEAAAREEARRSRALLATY
                     TNEKDIDAARTRALAEPQRQIADIEANIAQREKRLDELTKRKPAQNQSEIEAARSGIA
                     AQQELLAVKKQVVEAIKARYDEDKRRYQQLTRGR"
     gene            complement(2402..2485)
                     /locus_tag="VFF05_00075"
     CDS             complement(2402..2485)
                     /locus_tag="VFF05_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50229.1"
                     /translation="MVGQVMKVTGGRANPAQVNEILRRKLG"
BASE COUNT          469 a          796 c          832 g          401 t
ORIGIN      
        1 cgcgaagcga ttgccgggat gctcgcgcgc ggcgacgcgg cgacccgatg gctgcatgca
       61 gggacgggcg cgctcgccga gctcaggcgc cacccggcct ggatcgccgg cggcgtgggg
      121 ttgctgttcg cgctgcgtcc caagcgggtg ctgaaatggc tcgcgagcgg atggtcgctg
      181 tggcgcgtct atcgcggcgc gcgggcgtgg ctgtggcgcg cgattcctag agaggatatt
      241 cgtagtcgat gatcagcgga gcgtggtcgg agaagcgccg ccgcttgtag atggattccc
      301 tgacggcggt cttcgccacg gcgggcgtgg cgatctggta atcgatgcgc caaccgacgt
      361 ttttcgccca cgcctggccg cggttcgacc accacgtgta ctggtccggc ttgtggttca
      421 ggcgccggaa cacgtcgacc agcccgatct catcgaacac gcggctcagc caggcgcgct
      481 cctcgggcag gaagcccgag ttcttctgat tcgagcgcca gttgcgcagg tcgatttcct
      541 tgtgcgcgat attccagtcg ccgcacagaa tcacctcgcg ccgggcgcgc gcgagtttct
      601 tcagctgtgg cagaaaagcc tcgaggaagc ggaatttcga ggcctgccgg tgggggccgg
      661 aagagcccga gggcaggtaa agcgaaatga cggatagcct gccgaagcgc gcctcgaggt
      721 agcgcccctc cgagtcgaac tcgtgcgtgc cgaagcccct gctcaccgcg tcgggcgcac
      781 ggcgcgaata cagcgccacg ccgctgtagc ccttcttctc ggccagcgcg tagcttgccg
      841 cgaaccccct gggtgcatgc agcgcggcgt caaggtcgct ctcctgcgcc ttcacctcct
      901 gcaggcagat aacgtccgcg cgctgcgcgg aaagccagcg gaacaggcct ttccctgctg
      961 ccgatcgaat tccgtttacg ttaagcgtga tgacgcgcat tcaggacgac tgagagctcg
     1021 ccgcgccgcc atgcgccgcc gaccactcgg ccggcgcgtt taggaacttc tctacctctg
     1081 cgatctgcgc cgccggcagg taggcgccgc gcttgaccac cgccagcacg tcccaccagg
     1141 tcgcaaggtg atgcagccgc acgttcagat ccgccagaat cttcttcgac tcggggaaga
     1201 tgtcgtaata aaaattgacg aacacatggt caacgaccgc accggcattg cgcaaggcct
     1261 gacaaaaatt gatcttcgag cgcccgtcgg tggtgagatc ctcgaccagc agggtacgcg
     1321 cgccatcgcg tacgtcgccc tcgatctgcg cgttgcggcc gaagcccttt ggcttctttc
     1381 tgacgtattg catcggcaga ccgagccggt cggcgatcca cgcggcgtag ggtatgccgg
     1441 cggtctcgcc cccggcgacg acgtcgaact gctcgaatcc cgcgtcgcgc tgcagcgtag
     1501 cggcggcgaa gtcgaccagc tgcgaccgga tgcgcggata gaaaatgagc ttgcggcaat
     1561 cgatgtatac cgggctcgcc cagccggaag tgaaagtgaa cggcttatcg tccgaaaaca
     1621 gaaccgcctt cacctcgagc agcatcttgg cggtggtttc ggcgatgaag cttcggtcgg
     1681 cggagtgcat ggggtcacgc gtggagggat ggcgcggatt ttagcaggtg aacgcatgcc
     1741 tgccccgcgc tagaatattg ccgtcggcgt tccgcaaaag ggagaggcaa tgcgtcgctt
     1801 gaaactgttc gccgctgcgc tcacgatgct cgcattcgcc gcgatcccgc tggcggcgca
     1861 ggcgcaggcg tttcgctgcg tgggcaagga cggcaagaag tactacggcc agataatccc
     1921 gcaggcctgc atcggccagc cctacgagca gctgaacatg caggggatgg tgatcaagcg
     1981 cttcgacccg accgtcgaca atgtggaccc ggcgaccaag gcggccgagg aggaaaagca
     2041 gcgcgagaag gaggccgccg cgagggaaga ggcgcgacgc agccgcgcct tgctcgccac
     2101 ctacaccaac gagaaggata tcgacgccgc gcgcacacgc gcgctggccg aaccgcagag
     2161 gcagatcgcc gatatcgagg ccaatatcgc gcagcgcgaa aagcggctcg acgagctcac
     2221 caagcgcaag ccggcgcaga accagtcgga aatcgaggcc gccagatccg gcattgccgc
     2281 gcagcaggag ctgctcgccg tgaagaaaca ggtcgtcgag gccatcaagg cccgatacga
     2341 cgaagacaag aggcgctacc agcagctgac gcgcggcagg taacgaggca ggcgcgcggc
     2401 gctagccgag cttgcggcgc aagatttcgt tgacctgcgc cgggttcgcc cggccgccgg
     2461 tgactttcat cacctgccca accagagagt tgaaggcc
//
LOCUS       DASRVO010000006         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_311872, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000006 DASRVO010000000
VERSION     DASRVO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311872"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFF05_00080"
     CDS             complement(<1..401)
                     /locus_tag="VFF05_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZW50230.1"
                     /translation="MDQPVRIKGRGAASNPEGRFETVAREAVDDGWDRKPEPEPHPAT
                     QVTEERARGIISRNDSPDIPFEQSINPYRGCEHGCIYCYARPSHAYLNLSPGLDFETK
                     LFAKTNAAELLRSEWAKPGYRVSPINLGANT"
     gene            complement(445..1053)
                     /locus_tag="VFF05_00085"
     CDS             complement(445..1053)
                     /locus_tag="VFF05_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZW50231.1"
                     /translation="MEHLQALALAGGLAWASGIRLYAAVFIVGALGRLGYVHLPDHLE
                     LLEHNWVLGASGFMLLIEFVADKVPAIDSAWDAIHTFIRIPIGAMLAWGAMGDSAPDV
                     QMAAAILGGAIAGGTHLAKMGTRAAINTSPEPFSNWTLSFSEDGILVAGLWLVFRHPL
                     VFLILLAAFLLLLIWLLPKLFRFLAGLWRRLTGIGPRTLPAK"
     gene            1178..1435
                     /locus_tag="VFF05_00090"
     CDS             1178..1435
                     /locus_tag="VFF05_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50232.1"
                     /translation="MRWVVLLLALLGFALGFSSRSPGLMGLGFVLGFVLLIAAFFTFA
                     AARVEANSRPDSTLLTDKDIAMLRASMRKPPPTGTPPANPA"
     gene            1514..>2881
                     /locus_tag="VFF05_00095"
     CDS             1514..>2881
                     /locus_tag="VFF05_00095"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZW50233.1"
                     /translation="MATLHPVPADFAAHARIGAMDYEKLYAESVRDADGFWRRIGKRL
                     DWIVPYTKVRDVSFDAKDLHIRWYADGKLNLAANCLDRHLKTRGDKTAIVWEGDDPSE
                     SRAITYRQLHEQVCRAANLLKNLGIRKGDRVCIYLPMIPEAAVAMLACARIGAVHTVV
                     FGGFSPDSLAGRIADSQSKVVITADEGLRGSKHIPLKVNVDEALTRPGTTSVETVLVV
                     ERTGAPVPMQAPRDRSWNALVAVQSAECAPEPVGAEDPLFILYTSGSTGKPKGVLHTT
                     GGYGVFCAYTHECVFDLREDDVYWCTADVGWVTGHSYIVYGPLANGATSLMFEGVPNY
                     PDSGRFWQVIDKHQVTIFYTAPTAIRALMREGEAPVKKSKRTSLRLLGSVGEPINPEA
                     WEWYWRVVGDGRCPIVDTWWQTETGGILISPLPGAIAQKPGSATKPFFGVQPAIVDAS
                     GGVLEG"
BASE COUNT          491 a          937 c          949 g          504 t
ORIGIN      
        1 tcggtgttgg cgcccagatt gatcgggctc acgcgatagc ctggcttggc ccattcgctg
       61 cgcagcaatt ccgccgcgtt cgtcttggcg aacagcttcg tctcgaaatc gaggccgggc
      121 gaaagattca ggtaggcgtg cgagggccgc gcatagcagt agatgcagcc gtgctcgcag
      181 ccgcggtagg gattgatgct ctgctcgaag gggatatcgg gcgaatcgtt gcggctgatg
      241 atgccgcgtg cgcgttcttc cgtgacctgc gtggccggat gcggctcggg ttcgggcttg
      301 cggtcccagc catcgtccac ggcctcgcgc gcgaccgtct cgaagcggcc ttcgggattc
      361 gaggccgcgc cgcggccctt gatgcgaacg ggctgatcca tgcattgcat gctacgccgt
      421 gcgtgtctca gccgccgcga cgggctactt cgccggcagc gtgcgcggcc cgatgccggt
      481 cagtcgccgc cagaggccgg cgaggaaacg gaacagtttc ggtagcagcc agatcagcag
      541 cagcaaaaat gccgcgagca gaatcaggaa cacgagtggg tgccgaaaca ccagccacag
      601 gccggcgacg aggatgccat cctcggaaaa actcagcgtc cagttgctga agggttccgg
      661 cgaagtattg atcgctgcgc gcgttcccat ctttgccagg tgcgtgccgc cggcgatcgc
      721 accaccgagg atcgccgcgg ccatttgcac atccggcgcc gaatcgccca tcgcgcccca
      781 cgccagcatg gcgccgatcg gaatgcggat gaaggtgtgg atcgcatccc acgccgagtc
      841 gatggccgga accttgtcgg cgacgaactc gatcaacagc atgaagccgg aagcgccgag
      901 cacccagttg tgttcgagca actccagatg gtcgggcaga tgcacgtagc ccaggcgtcc
      961 gagcgcgccg acgatgaaga cggccgcgta caggcggatg ccactggccc aggccaggcc
     1021 gccggccaaa gccaatgctt gcagatgttc catggttctt ccgggcctca accactggtc
     1081 ggaaattcta cgcgttcggt ctgaaccgcg ccgcaccgct ttcgcgccgg gccgaaactg
     1141 tggcaggatt ttgccgtcgc gacgttcggg gatgcggatg cgctgggttg tgctgctgct
     1201 tgcgctgttg ggtttcgcgc tggggttctc atcgcgatcg ccgggcctga tgggtttggg
     1261 cttcgtgctc ggcttcgtat tgctcatcgc cgccttcttc accttcgccg ccgcgcgtgt
     1321 ggaggcgaac agccgtccgg actcgaccct gctcaccgac aaggacatcg ccatgctgcg
     1381 cgcgagcatg cgcaagccgc cgccaacggg cacaccgccg gcgaatccgg cctgagcgca
     1441 cgccgcccta cgcgggtttc ccgcgtcatc gaatcggaat agagtgcggt tttagcttcg
     1501 gtagaccgcc gccatggcca ccttgcatcc cgttcccgcc gatttcgccg cgcatgcgcg
     1561 catcggcgcg atggattacg aaaaactcta cgccgaatcc gtgcgcgatg cggacggatt
     1621 ctggcgccgc atcggcaagc gcctggactg gatcgtgccg tacacgaagg tgcgcgacgt
     1681 ttccttcgac gcgaaggacc tgcacatccg ctggtacgcc gacggcaagt tgaacctcgc
     1741 cgcgaattgc ctggaccgac acctgaagac gcgcggcgac aagaccgcga tcgtgtggga
     1801 aggcgacgat ccttccgagt cgcgcgcgat cacctaccgg cagttgcacg aacaggtctg
     1861 ccgtgccgcg aacctgttga agaacctcgg catccgcaag ggcgaccgcg tttgcatcta
     1921 cctgccgatg attcccgagg ccgcggtggc gatgctcgcc tgcgcgcgca tcggcgcggt
     1981 gcacaccgtc gtgttcggcg gcttctcgcc ggattcgctg gcagggcgca tcgccgattc
     2041 gcaatcgaaa gtcgtgatca ccgccgacga aggcctgcgc ggtagcaagc acattccact
     2101 caaggtgaac gtcgacgaag cgctcacccg tcccggcacg accagtgtgg agaccgtgct
     2161 cgtggtcgag cgcactggtg cgccggtacc gatgcaggcg ccgcgcgacc gctcatggaa
     2221 cgcgctcgtc gccgtgcaaa gcgccgaatg cgcgccggag ccggtgggcg ccgaggaccc
     2281 cttgttcatt ttgtatacct cgggttcgac gggcaaaccc aagggcgtac tgcacacgac
     2341 cggcggttac ggcgtgtttt gcgcctacac ccacgaatgc gtgttcgacc tgcgcgagga
     2401 cgatgtctac tggtgcaccg cggacgtcgg ctgggtcacc ggccacagct acatcgtcta
     2461 cggaccgctt gccaacggcg ccacctcgct gatgttcgag ggcgtgccga attacccgga
     2521 ttcgggtcgc ttctggcagg tcatcgacaa gcaccaggta acgattttct acactgcgcc
     2581 gaccgcaatc cgcgcactga tgcgcgaggg cgaggcgccg gtgaagaaat ccaagcgcac
     2641 ctccttgcgc ctgttgggct cggtcggcga gccgatcaac ccggaagcct gggaatggta
     2701 ctggcgcgtg gtcggcgacg gccgctgtcc gatcgtggac acctggtggc agaccgagac
     2761 cggcggcatc ctgatctcgc cgctgccggg cgcgatcgcg cagaagcccg gttcggcgac
     2821 caagccgttt ttcggcgtgc agccggccat cgtcgatgcg agcggcggcg tgctcgaggg
     2881 c
//
LOCUS       DASRVO010000007         1927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_26127, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000007 DASRVO010000000
VERSION     DASRVO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26127"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1702
                     /locus_tag="VFF05_00100"
     CDS             <1..1702
                     /locus_tag="VFF05_00100"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZW50234.1"
                     /translation="SAPRVKHASANNDADAPEEATQTGSQASAPTPGSGASPRRPGAT
                     PAAGGHMPPPGIFTPPGTYPPPGMGMRAVAPDDRDAELFTTLRELLAGRRSALGVSDS
                     APSNGYMPSSEDLQSVLNTLQAKPISPMMMGGKLVQRSVGHLKQDLLSQLRQHAPEGK
                     APQLAAEDSDTIDLVGMLFDYLTKNVRPDGSTQALMTKLQVPLLRVALRDKNFFTRRA
                     HPARQLLNAIAETGTHWLDDSEGPADRALVDKMQVVVDKVSQEFDGNLGLIENMLTDL
                     SKHMHTLARKAEVTERRHVDAAKGREKLSVAREQAQAAISQRIAAAKPNKLVRTLLEQ
                     AWTDVLALTLLRQGENSETYAKRLNVADQLIAATSARSGEIKAPTELRSEIETALGQV
                     GYHDDDVQAVVKRLFSPGQADVGEEAVTSTEIAIKLKSKARLGEDNTAPAATPAPAAR
                     KKKTFEPDSAEARALERLKTLPFGTWFEFRTNQQGDKVRRKLAWFSTLTGHCLFVNQR
                     GVRVDEKDLEQLARDLVGGQAWIVEPETETLVDRAWKAIMSSLRQLVGRDPEPVPVPA
                     "
     gene            1699..>1927
                     /locus_tag="VFF05_00105"
     CDS             1699..>1927
                     /locus_tag="VFF05_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50235.1"
                     /translation="MRSSQQAPAMSEHRRNTRKRAHHAIAVNDAIGGHQIGYVGNLSV
                     DGMLLISSRQFVENALFQVNFELPNGATTKSH"
BASE COUNT          421 a          666 c          585 g          255 t
ORIGIN      
        1 gagcgcaccg cgcgtcaagc atgccagcgc caataacgat gcggatgcgc ccgaagaagc
       61 gacacagacc ggatcgcagg ccagcgcacc gacgccggga agcggcgcct cgccgcgtcg
      121 accgggcgcc actcccgccg cgggcggcca catgccgcca ccgggaatct tcaccccgcc
      181 gggcacctac ccgccgcccg gcatgggcat gcgcgcggtt gcgcccgacg accgcgatgc
      241 ggaattgttc accacgctgc gcgagttgct ggccggccgg cgcagtgcgc tcggcgtcag
      301 cgacagcgca ccatccaacg gatacatgcc aagcagcgaa gatctgcaat cggtgctgaa
      361 caccctgcag gccaagccaa tctcgccgat gatgatgggc ggcaagctcg tgcagcgctc
      421 ggtcgggcat ctgaaacagg acctgctttc gcaactgcgc cagcacgctc cggaagggaa
      481 ggcgccgcaa ctggcggccg aagattcgga caccatcgac ttggtcggca tgctattcga
      541 ctacctgacc aagaacgtgc gcccggatgg cagcacgcag gcgttgatga cgaaattgca
      601 ggtgccgctg ctgcgcgtgg ccctgcgcga caagaacttc ttcacccgcc gcgcgcatcc
      661 ggcgcggcaa ttgctcaatg cgatcgccga aaccggcacg cattggctgg acgacagcga
      721 aggcccggcc gatcgcgccc tggtcgacaa gatgcaagtg gtggtggaca aggtcagcca
      781 ggaattcgat ggcaatctcg gcctgatcga gaacatgctc accgacctgt ccaagcacat
      841 gcacacgctg gcgcgcaagg ccgaagtgac cgagcgccgc catgtggacg ccgccaaggg
      901 ccgcgaaaaa ctctccgtcg cgcgcgaaca ggcgcaagcc gccatctccc aacgcattgc
      961 cgcggccaaa cccaacaagc tggtgcgcac cctgctcgaa caggcctgga cagacgtgct
     1021 cgcgctcacc ctgctgcgcc agggcgagaa cagcgagacc tacgccaagc gcctgaacgt
     1081 ggcggatcaa ttgatcgccg ccaccagcgc acgcagcggc gaaatcaagg cgccgaccga
     1141 actgcggagc gaaatcgaaa ccgcgctggg tcaagtcggc tatcacgacg acgacgtgca
     1201 ggcggtggtc aaacgcctgt tctcgccggg ccaagccgat gtcggcgaag aagccgtcac
     1261 cagtaccgaa atcgcgatca agctcaaaag caaagcgcgc ctcggcgagg acaacaccgc
     1321 acccgccgcc acacctgcac cggccgcgcg caagaaaaag acgttcgagc cggacagcgc
     1381 ggaagcgcgc gcgctggaac gcctgaagac gctgccgttc ggcacctggt tcgagttccg
     1441 caccaaccag caaggtgaca aggtgcgccg caaactggca tggttctcga cgctcaccgg
     1501 tcactgtctg ttcgtcaacc agcgcggcgt gcgtgtagac gagaaggatc tcgaacagct
     1561 cgcgcgcgac ctcgtcggcg gtcaggcgtg gatcgtggaa ccggaaaccg aaaccctagt
     1621 cgaccgcgcc tggaaagcaa tcatgtcctc gctcagacag ttggtcggac gcgaccccga
     1681 acccgtgccg gtacccgcat gagatcctcg cagcaagcac ccgccatgtc cgaacatcgc
     1741 cgcaacacac gcaaacgagc ccaccacgcc atcgccgtca acgacgcgat cggcggccat
     1801 cagatcggct atgtcggcaa tctttccgtc gatggcatgc tcctgatcag cagccgccag
     1861 tttgtcgaaa acgcgctgtt ccaggtcaat ttcgagttgc cgaacggcgc caccacgaag
     1921 agccaca
//
LOCUS       DASRVO010000008         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_233988, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000008 DASRVO010000000
VERSION     DASRVO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233988"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2558
                     /locus_tag="VFF05_00110"
     CDS             <1..>2558
                     /locus_tag="VFF05_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW50236.1"
                     /translation="PVALRDFATLELAPALSAVSGVADIEVLGGRTGEFQVLLDPARL
                     RAHGLAAADVTQALNAGNAVDAVGRIEDRYRLYLTIADSRLRGVDDIRHTVVKTVNGQ
                     PITIADLGSVQLGQQPEWTRVTANGGDAVLINIRQQRGANTVALVRDVRTRLAQLRTQ
                     IPSDIRVGTYYDQSELIVASADSVRDAILIGALFAALILFMFLRSARVTLIAAVTLPV
                     VIAITVLLLHVFGMSFNIMTLGGMAAAVGLIVDDAVVMIEHIMRRAGAERAAGDATVL
                     RAAQEMLRPLTGSSLATIVVFAPLAFLGGVAGGFFKALALTMACGLAVSWALAALALP
                     LVAARLIRVDDAQRMEQAERWIAGTQRRYATRMRRLLARPRWALPAIAICVVVAALCY
                     WRLGTGFMPQMDEGGFVIDYVSVPGTSLAETDRLLRQVEAIIKSTPEVDDYSRRTGAQ
                     LGGGLTEANEGDFFVHLKPAPRRGIVDVMSEVRTKVEAQVPGLRIETAQLMEDLIGDL
                     VATPQPVEVKVFGQNPATDRAEAAKIAAAIAKIPGVVEVFDGVKIAGDAIEVRVDRVR
                     AALDGLDPDAVTHQLETELGGSLAGSIRQGEKLVGIRVWTPQDLRQRIAQLAALPLRA
                     ADGHTLPLGRVADISIAEGQPEADREDQRPLAAVTARLEGRDLGSAMNDVKASVAKMK
                     LPIGVQVEYGGLYGQQQDSFRAMALVFAGALLLIITLLMFMYENIAVVASILVATFCS
                     LAGVFAGLWLTNTELDLSSIMGLTMILGIVTEIAIFYFAEIDFVRRPDLAALIEAGSM
                     RMRPILMTSLIAILALSPLALGIGTGSAMQQPLAIAIVAGLIAAVPIVLILMPA"
BASE COUNT          402 a          838 c          856 g          462 t
ORIGIN      
        1 tccggtcgcg ctgcgcgatt tcgccacgct ggaactggcg cctgcgctga gcgcagtctc
       61 cggcgtcgcc gacatcgagg tgctgggcgg gcgcaccggc gagtttcagg tactgctcga
      121 tcccgcgcgg ctgcgcgcgc atggactcgc cgccgccgac gtgacgcagg cgctcaacgc
      181 cggcaatgcg gtcgatgcgg tgggtcgcat cgaggatcgc tatcgcctgt acctgaccat
      241 cgccgattcg cgcctgcgcg gcgtcgacga catccgccac acggtcgtga aaacggtgaa
      301 cggccagccg atcacgatcg cggatctggg cagcgtgcaa cttggccagc agccggaatg
      361 gacgcgcgtc accgcgaatg gtggcgacgc cgtgctgatc aacatccgtc aacaacgcgg
      421 cgcgaatact gtggcactgg tgcgcgatgt gcgcacgcgc ctggcgcagt tgcgcacgca
      481 gatcccgtcc gatatccgcg tcggcaccta ttacgaccag tccgaactca ttgtcgcctc
      541 ggcggacagc gtgcgcgacg cgatcctgat cggcgccctg tttgccgcac tgatcctgtt
      601 catgttcctg cgcagcgcgc gcgtcaccct gatcgcggca gtgactctcc ccgtcgtcat
      661 tgcgatcacc gtgctgctgc tgcacgtctt cggcatgagc ttcaacatca tgaccctcgg
      721 cggcatggcc gcggcggtcg ggttgatcgt ggacgacgcg gtggtgatga tcgaacacat
      781 catgcgtcgc gccggcgcgg aacgcgcggc aggcgatgcc accgtgctgc gcgccgcgca
      841 ggaaatgctg cgcccgctga ccggttcatc gctggcgacc atcgtcgtgt tcgcgccgct
      901 cgcgtttctc ggcggcgtcg ccggcggttt cttcaaggcg ctggccttga ccatggcttg
      961 tggattggcg gtttcctggg cgctggccgc gctcgctctg ccgctggtcg ccgcacgcct
     1021 gatccgcgtc gacgacgcac aacgcatgga gcaggccgag cgctggatcg ccggcacgca
     1081 gcgccgctac gccacgcgca tgcgtcgcct gctggcgcgt ccgcgctggg cgttgcccgc
     1141 catcgcaatt tgcgtggtcg tcgccgcatt gtgctactgg cgcctcggca ccggcttcat
     1201 gccgcaaatg gacgaaggtg gcttcgtgat cgattacgtc agcgtacccg gtacctcgct
     1261 ggccgaaacc gaccgcctgc tgcgccaggt cgaggcgatc atcaagtcca cgcccgaagt
     1321 cgacgattat tcgcgccgta ctggagcgca actcggcggc ggcctgacag aagcgaacga
     1381 gggcgatttt ttcgtccacc tgaagcctgc gccgcgacga ggcatcgtag acgtgatgag
     1441 cgaggtgcgg accaaggtcg aagcacaggt gccgggcctg cgcatcgaaa ccgcgcaatt
     1501 gatggaagac ctgatcggcg acctcgtcgc cacaccccag cccgttgaag tcaaggtttt
     1561 cggccagaat ccagccaccg accgcgcgga agcggcgaaa atcgccgccg cgattgctaa
     1621 gatacccggc gtggtcgagg ttttcgacgg agtcaagatc gccggcgacg ccatcgaggt
     1681 tcgcgtcgac cgcgtgcgcg ccgcgctgga tggactcgat ccggacgcgg taacgcacca
     1741 gctcgaaacc gagctgggcg gcagcctcgc cggttcgatc cggcagggcg agaaactggt
     1801 cggcatccgc gtctggacgc cgcaggatct gcgtcagcgc atcgcgcaac ttgctgcgct
     1861 gccgcttcgc gcggcggatg ggcatacgct gccgctgggg cgggtggcgg atatttccat
     1921 cgcggaaggc cagcccgagg ccgaccgcga ggaccagcgg ccgctggcgg cggtgacggc
     1981 acggctggaa ggacgcgatc ttgggtcggc gatgaacgat gtgaaggcga gcgtggcgaa
     2041 gatgaaattg ccgatcggcg tgcaggtcga gtatggcggc ctgtacgggc agcagcagga
     2101 ttcgttccgc gcgatggccc tggtgttcgc cggtgcgctg ttgctgatca tcaccctgct
     2161 catgttcatg tacgagaaca tcgccgtggt cgcgagcatc ctcgtcgcga cgttctgttc
     2221 gctcgccggc gtgttcgcgg gactgtggct gacgaatacc gaactggacc tttcatcgat
     2281 catgggcctg acgatgatcc tgggtatcgt caccgaaatc gcgatcttct atttcgccga
     2341 aatcgatttc gtgcgccggc ccgacctggc tgcgctgatc gaagccggca gcatgcgcat
     2401 gcgcccgatc ctgatgactt cgctgatcgc catcctcgcg ttgtcgccgc tcgcgctcgg
     2461 catcggcacg ggttcggcca tgcaacagcc gctggcgatc gcgatcgtcg ccggtctgat
     2521 cgccgccgtg ccgatcgtgc tgatactgat gccggcgg
//
LOCUS       DASRVO010000009         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_234011, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000009 DASRVO010000000
VERSION     DASRVO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234011"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..>1091)
                     /locus_tag="VFF05_00115"
     CDS             complement(49..>1091)
                     /locus_tag="VFF05_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZW50237.1"
                     /translation="RVDDPTRYRMVSAPDYVLEPALDPLHRRLALQLPAGYDARARAL
                     AQRWRDQFHGNANLVIRSALDLFHNEFSYTLNAPLLGRNSVDDFLFDTKRGFCEHFAS
                     SFTFLMRAAGVPARVVTGYQGGYFNKLGNYWVVRQSDAHAWSEVWLEGRGWVRVDPTA
                     AVSPQRVQVGASAAAGASAPWYQADWLMGLRNRLDLVNRFWNDAIVQFNALRQQSVLT
                     PFGVSKADYPQLMLAMIAFGAVLLGLFAWWVMRAPKPAGDELDAAYAKFRHKLARAGV
                     AHDPAEGPAALGRRADARLAAPQAVQRLLADYVGLRYACALPAPERIQTFTRAVAALR
                     LPVATQSARQSS"
BASE COUNT          192 a          345 c          384 g          170 t
ORIGIN      
        1 ggtcaaggtg gatctgcgca tggcggtccc tccggcttct gcatcctgtc aggacgattg
       61 tcgcgccgat tgcgtcgcga cgggtagcct caacgcggcg acggcgcgcg taaaagtttg
      121 tatccgttca ggtgcgggca acgcacaggc atagcgcagg ccgacatagt cggcgagcaa
      181 gcgctgcacg gcctgcggtg cagcgagtcg tgcatcggcg cggcggccca gggccgcagg
      241 tccctcggcc ggatcgtgcg ccacgcccgc gcgcgcgagc ttgtgccgaa atttcgcata
      301 cgcggcatcg agttcatcgc cggcaggttt gggcgcgcgc atgacccacc aggcgaacag
      361 gcccagcaac acggcgccga acgcgatcat cgccagcatc agttgcggat agtcggcctt
      421 ggatacgccg aatggcgtga gcacgctttg ctggcgcagt gcgttgaact ggacgatggc
      481 gtcgttccag aaacggttga ccaggtccag gcgattgcgc agacccatca gccagtccgc
      541 ctggtaccac ggcgcgctgg cgccggccgc cgcgctggcg ccgacttgca cgcgttgcgg
      601 actgactgcg gcggtcgggt cgacgcgcac ccagccgcgg ccttccagcc agacctcgga
      661 ccaggcgtgc gcatcggact ggcgcaccac ccagtagttg cccagcttgt tgaagtaacc
      721 gccctggtaa ccggtgacca cgcgcgccgg aacgccggcg gcgcgcatca ggaacgtgaa
      781 tgacgaggcg aaatgttcgc agaagccacg tttcgtatcg aacaggaaat cgtccaccga
      841 attgcggccc agcaggggcg cgttgagggt gtaggaaaac tcgttgtgga acaggtccag
      901 cgcggagcgg atgacgagat tggcgttgcc gtggaattga tcgcgccagc gttgcgcgag
      961 cgcgcgtgcc cgcgcatcgt atccggccgg caattgcagg gcgaggcgcc ggtgcagcgg
     1021 atcgagcgcg ggttcgagca cgtagtccgg cgccgaaacc atgcggtagc gcgtcggatc
     1081 gtccacgcgc g
//
LOCUS       DASRVO010000010         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_182049, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000010 DASRVO010000000
VERSION     DASRVO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182049"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VFF05_00120"
     CDS             <1..250
                     /locus_tag="VFF05_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HZW50238.1"
                     /translation="EELFEAVTLKRLGLYFNPIVLLNTHDFYTPLQAFMNKVIAERFM
                     NPEHAAMWSLVNAPEDVLPEIQRTPKWREDARDYAVVR"
     gene            complement(274..>1500)
                     /locus_tag="VFF05_00125"
     CDS             complement(274..>1500)
                     /locus_tag="VFF05_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HZW50239.1"
                     /translation="NQAWFEYQPARVLPPGSKLDTFNAPHVVDTLVKDFDDNGLGTEP
                     NNLAAINSLIGYRALRWGRHIDLIVTDQRSYRSRDPGNHDEINPLFEGDKLGFVPEEL
                     WAQLDAGREYANGHPPAKLSFGGKSVDNYRANEAPQTMLGAKQKAWFLERLRGAKATW
                     KIWGTSLGTPDQRVDPQNLPAALGKWNGAGYALAGTWDWGAMYHERAEIFDAVRDAGI
                     TGFAIVAGDRHSFWAGYAAKALPPAAFEPVGVTFVGGSISSVGSAEANEHRFKKDDPL
                     RPLYVANINGGPPQCTENLLLRHGVRSALEYASSGSLEKAHAVSNPELAPHLTFVDAG
                     GHGYATVRVDASAMVTEFVCIPRPIERSTSADGGPLRYRVRHEVPLWRAGERPLMRQT
                     VVEGDAGLSGVSRFPA"
BASE COUNT          257 a          477 c          484 g          282 t
ORIGIN      
        1 ggaagaattg ttcgaagcgg tcacgctcaa gcgcctaggt ttgtatttca accccatcgt
       61 cctgctcaac acccatgatt tctacacgcc gctgcaagcg ttcatgaaca aggtgatcgc
      121 cgagcgtttc atgaatcccg agcacgcagc gatgtggtcg ctcgtgaacg cgcccgaaga
      181 cgtgctaccc gaaatccaac gcacgccgaa gtggcgcgag gatgcacgcg attacgcggt
      241 ggtgcggtga gcgaatcttg aaggcagtcg acctcaagcc gggaacctgg agacacccga
      301 tagacccgca tcaccttcca cgaccgtctg ccgcatgagc ggcctttcgc cggctcgcca
      361 gagcggcact tcgtggcgga cgcgatagcg caacgggccg ccgtccgcgc tcgtgctgcg
      421 ttcgatcggc cgcgggatac agacgaactc ggtcaccatc gcgctggcgt cgacgcgcac
      481 ggtggcgtag ccgtggccgc ctgcgtcgac gaaggtcaga tgcggggcca actccggatt
      541 gctcacggcg tgcgccttct ccagtgaacc gctgctcgcg tattccagcg ccgagcgcac
      601 gccgtggcgc agcagcaagt tttcggtgca ctgcggaggt ccgccgttga tgttcgcaac
      661 gtacagcggg cgcagcggat cgtctttctt gaatctgtgc tcgttggctt cggccgagcc
      721 aacggaagag atcgacccgc cgacgaaggt gacgcccaca ggctcgaatg cggcgggcgg
      781 caaggccttc gccgcgtagc ccgcccagaa gctgtggcga tcgccggcga cgatcgcgaa
      841 accggtgatg cccgcatcgc gcaccgcgtc gaagatttcg gctcgctcgt ggtacatggc
      901 gccccaatcc caagtgcccg cgagggcgta gcccgcgcca ttccatttgc cgagtgcggc
      961 agggagattc tgcggatcga cacgctggtc gggcgtgccc agtgaagtgc cccagatttt
     1021 ccaagtggct ttcgcgccgc gcaggcgctc gaggaaccag gctttctgct tcgcgccgag
     1081 catcgtctgc ggtgcttcgt ttgcgcggta gttgtccacg ctcttgccgc cgaagctcag
     1141 cttcgccggc ggatgaccat tggcgtactc gcggccggcg tcgagctgcg cccacaattc
     1201 ctcgggcacg aagccgagct tgtcgccttc gaacaacgga ttgatttcgt cgtggttgcc
     1261 gggatcgcgc gagcggtagc tgcgctggtc ggtgacgatc aggtcgatgt gccggcccca
     1321 gcgcaaggcg cgatagccga tcaggctgtt gatcgcggcc aggttgttcg gttcggtgcc
     1381 cagtccattg tcgtcgaaat ccttgaccaa cgtgtcgacc acatgcggcg cattgaacgt
     1441 gtccagcttc gagcccggcg gcaacacgcg tgcgggttgg tattcgaacc aggcttggtt
//
LOCUS       DASRVO010000011         1097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_225, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000011 DASRVO010000000
VERSION     DASRVO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /gene="cca"
                     /locus_tag="VFF05_00130"
     CDS             <1..311
                     /gene="cca"
                     /locus_tag="VFF05_00130"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HZW50240.1"
                     /translation="LELRPKTVLELFERLDAFRQPRRLPQFLLACAADKRGRAGMADS
                     DYPQARYLTELFHAALEPRAQALLDAGLRGPELGAALREARRKAIAAAIRAHKKNPAA
                     "
     gene            complement(345..>1097)
                     /locus_tag="VFF05_00135"
     CDS             complement(345..>1097)
                     /locus_tag="VFF05_00135"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HZW50241.1"
                     /translation="ANGTISGTSPQTVNYGGNGSLVTAMPNTGYHFVQWSDGVLTAAR
                     TDTNVQANISVTATFAIDQFTLTYAAGTGGSINGNSSQTVDYQGNGTAVGAVPDAGYH
                     FVQWSDGITDNPRADANVTADVDVTAQFAANVLVFTVQPADVALGNTLATVEVTEQDG
                     SGNTIVDNATVGLTLVTVCGTLDLGSVQMVDGVATLNSSQRFYTLHSGYQITATVTNP
                     NPTPMVAVLSATFDATASAELVFSDGFESCRP"
BASE COUNT          185 a          369 c          331 g          212 t
ORIGIN      
        1 cgctcgagtt gcgcccgaaa accgtgctgg aacttttcga gaggctcgac gcgtttcgcc
       61 aacccaggcg tctgccgcaa ttcctgctcg cctgtgccgc cgacaagcgc ggacgtgcag
      121 gcatggccga cagcgattat ccgcaggcgc gctatctcac cgagctattt cacgccgcgc
      181 tggaacccag agcacaggcc ttgctcgatg ccggcttgcg cggcccggag ttgggtgccg
      241 ccttgcgcga agcgcgacgc aaggcgattg ccgccgccat tcgcgcgcac aaaaaaaatc
      301 ccgcggcgtg agccgcggga tcgggtttgc accgtaaaga cgggttatgg tcggcagctt
      361 tcgaagccgt cgctgaatac cagctccgcg ctggcagtcg cgtcgaacgt cgcgctcaga
      421 accgccacca tcggcgtcgg gttcggattc gtcaccgtcg ccgtgatctg gtagccgctg
      481 tgcagggtat agaaccgctg cgaactgttc agcgtggcca cgccatcgac catctgcacg
      541 ctgcccaggt ccagtgttcc gcagaccgtg accagggtca ggcccaccgt ggcgttgtcg
      601 acgatcgtgt tgccgctgcc atcctgctcg gtcacctcga ctgtagccaa cgtgttgcct
      661 agggcgacat ccgccggctg caccgtgaat accagcacat tcgcggcaaa ttgcgccgtc
      721 acgtccacat cagcagtcac gtttgcgtcg gcgcgcgggt tgtccgtgat cccgtcgctc
      781 cattgcacga aatggtaacc ggcatccggc acggctccca cggccgtgcc attgccctgg
      841 tagtcgactg tctgcgagct attgccattg atcgaaccac ccgtgcctgc cgcgtaggtc
      901 agcgtgaact ggtcgatcgc gaatgtcgcc gtgacagaga tgttcgcctg cacgttcgta
      961 tccgtacgcg cggccgtcag cacgccatcg ctccattgca cgaagtgata gccggtgttc
     1021 ggcatcgccg tgaccaggct gccgttgcca ccatagttca ccgtctgcgg cgacgtgccc
     1081 gagatcgtac cgttcgc
//
LOCUS       DASRVO010000012         1312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_26223, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000012 DASRVO010000000
VERSION     DASRVO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26223"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VFF05_00140"
     CDS             <1..587
                     /locus_tag="VFF05_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HZW50242.1"
                     /translation="VISNGERSEVRFNLIAEAQADPALPVTLVLSIYKFDCMEWAIEK
                     ATELGVAAIAPVVARRTDKHLAHAAEKRAERWRRIAHEAAQQSRRSDVPLIEMPAPLA
                     EWVCIESSAHRVVLAEQERSTTLRSLVEEAAESAGDELPALEIAIGPEGGWAPDEEAL
                     FDANGWKAATLGPRILRAETAAIAALAVIASCLE"
     gene            complement(594..>1312)
                     /locus_tag="VFF05_00145"
     CDS             complement(594..>1312)
                     /locus_tag="VFF05_00145"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HZW50243.1"
                     /translation="ALQCGGSDGFSGVSANPTIGYVSDLIAGLGGTTILSEFPELHGV
                     EQELINRCATDELAERFARLMQDYADRAKAAQSSFAFNPSPGNIREGLITDAMKSAGA
                     ARKGGLSTIRGVLDHPEYATTPGLNLQCTPGNDIESVTAQVGAGATIVLFTTGLGTPT
                     GNPIAPVIKVSTNSTTGSHMSDIIDFDAGVIVSGGATVKDCGEQLFEMTIDVASGKRQ
                     SKAEMRGQIDFIPWKRGVSL"
BASE COUNT          223 a          383 c          463 g          243 t
ORIGIN      
        1 ccgtgatctc caatggcgag cgcagcgagg tgcgcttcaa cctgattgcc gaggcgcagg
       61 cggacccggc gctgccggtt acgctggtac tgtccatcta caagttcgat tgcatggaat
      121 gggcgattga gaaggcaacc gagttgggcg ttgccgcgat tgcgcccgtg gtcgcccgcc
      181 gcaccgacaa gcacctggcg catgccgcgg agaagcgcgc ggagcgctgg cggcggatcg
      241 cgcatgaggc ggcgcagcag tcgcggcgca gtgacgtgcc cctgattgag atgccggcgc
      301 cgcttgccga atgggtttgc atagagtctt cggcgcatcg cgtcgtgctt gcggagcagg
      361 aacgctcgac gacgctgcga agcctggttg aggaagccgc tgaatccgcc ggcgacgagc
      421 tgccggcgct cgagatcgct atcgggccgg aaggcggatg ggcgccggac gaggaagcat
      481 tgttcgatgc gaatggatgg aaggcggcaa cgctggggcc gcgcatcctg cgcgcggaga
      541 cggcggcgat tgcggcgctg gcggtgattg cgtcgtgcct ggagtagccg acgctataaa
      601 gacacgccac gtttccaggg aataaagtcg atctggccgc gcatctccgc cttggactgg
      661 cgcttgccgc tggcgacatc gatggtcatc tcgaaaagct gctcgccgca atctttcacc
      721 gtcgctccgc cgctcacgat cacgccggcg tcgaagtcga tgatgtcgct catatgcgag
      781 ccggtggtgc tgttggtcga gactttgatc actggcgcga tggggttgcc ggtgggcgtg
      841 cccaggccgg tggtgaagag gacgatggtg gcgcctgcgc caacttgcgc cgttacgctt
      901 tcaatgtcgt tgcctggcgt gcattgcagg ttaaggcccg gcgtagtcgc atactcggga
      961 tggtcgagga cgccgcggat ggtggaaagg ccgcctttgc gcgcggcgcc ggctgacttc
     1021 atggcgtcgg tgatcagccc ttcgcgaata ttgcccggcg atgggttgaa tgcaaaactc
     1081 gactgcgcgg ctttggcgcg gtcggcgtaa tcctgcatca ggcgcgcaaa acgttcggcc
     1141 agttcatcgg tggcgcagcg gttgatcagc tcctgctcga caccgtgcaa ctctgggaat
     1201 tcagagagga ttgtcgttcc gcccagcccg gcgatcagat ccgagacata gccgatggtg
     1261 ggattcgccg acacgccgct gaagccgtcg gatccgccgc actgcaaagc ca
//
LOCUS       DASRVO010000013         1353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_312019, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000013 DASRVO010000000
VERSION     DASRVO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312019"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFF05_00150"
     CDS             <1..1179
                     /locus_tag="VFF05_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HZW50244.1"
                     /translation="EVQIRTEEMHRGAELGVAAHWRYKEGGGADAEFERKIAWMRQLL
                     EAKGEADDDGALLAGFRTDILEDRVYLLTPQGQVLDLAQGATVLDFAYHVHTDVGHRC
                     RGAKVNGRIVPLSFQPVSGDRVEILTGKAIEPRRDWLSSQHGFLNTHRAQQKVRAWFR
                     HVDQNANLAAGRAILDKELKRLALGNVELDSLPQKFQQQTLEDFLVALALGEITAGQV
                     ARALHETHTPPPPPTSAPPRAPRRGKDAVVIEGVGNLLTALARCCQPLPGDAIAGYIT
                     RGKGVSIHRADCASFARLRARDPARAIEVEWGGGRAQAYDVDVLVRGYDRKWLHKDVT
                     NVIAAANVHLIAVNTRVDAASGLATMNFAVRVADFEQLSMLLARLAAVPNVIEARRLA
                     "
BASE COUNT          223 a          454 c          462 g          214 t
ORIGIN      
        1 gaagtgcaga tccgcaccga ggagatgcac cgcggcgccg aactcggcgt ggccgcgcat
       61 tggcgctaca aggaaggcgg cggcgcggat gccgagttcg agcgcaagat cgcctggatg
      121 cggcaactgc tcgaagccaa gggcgaggcc gacgacgacg gcgcactgct cgccggattc
      181 cgcaccgaca ttctcgagga tcgcgtctac ctgttgacgc cgcagggaca ggtgctggac
      241 ctggcgcagg gcgccacggt cctggatttc gcctatcacg tgcacaccga cgtcggccac
      301 cgctgccgcg gcgccaaggt caatggccgc atcgtgccgc tgagtttcca gccggtcagc
      361 ggcgatcgcg tggaaatcct caccggcaag gcgatcgaac cgcgccgcga ctggctctcg
      421 tcgcagcatg gattcctcaa cacccatcgc gcccagcaga aagtgcgcgc gtggttccgg
      481 catgtggacc agaacgcaaa cctggcggcg gggcgcgcga ttctcgacaa ggagctcaag
      541 cgcctcgcgc tcggcaatgt ggaattggat tccttgccgc aaaagttcca gcagcagacg
      601 ctggaggatt tcctcgtcgc attggcgctc ggcgaaatca ccgcgggcca ggtcgcgcgt
      661 gccttgcacg agacgcatac gccgccaccg ccgccgacat ctgcgccgcc gcgcgcgcct
      721 cggcgcggca aggacgcggt cgtcatcgaa ggcgtcggca atctgctgac ggcgctcgca
      781 cgctgctgcc agccgttgcc cggcgacgcc attgccggtt acatcacgcg cggcaagggc
      841 gtgtcgattc atcgtgccga ctgcgccagc ttcgcgcgcc tgcgcgcgcg cgacccggcc
      901 cgcgccatcg aggtcgaatg gggcggaggc cgcgcccagg cttacgacgt cgacgtgctg
      961 gtgcgcggct acgaccgcaa gtggctgcac aaggacgtga ccaacgtcat cgcggcggcg
     1021 aacgtgcacc tgatcgcggt gaacacgcgc gtggatgcgg cctcgggcct ggcgaccatg
     1081 aactttgccg tgcgcgtggc cgatttcgag cagttgtcga tgttgctggc gcgtctggcg
     1141 gcggtgccga acgtgatcga ggcgcggcgc ctggcctagg catggcatgt cgtgcagggc
     1201 acgaagtgtc gcaaaagtcc gccgtaaggc gttcctcgtg ccaagaccct gacatgaggt
     1261 tcgccatgcg tcgtcatcca ggcacggttc tgcccaaacc ggctgcattg ccggctgcca
     1321 tcgcgctgct gctcgttgcc cccggggccg ttg
//
LOCUS       DASRVO010000014         1259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_234111, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000014 DASRVO010000000
VERSION     DASRVO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234111"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFF05_00155"
     CDS             <1..312
                     /locus_tag="VFF05_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HZW50245.1"
                     /translation="FMQNQLDAVVTSRVGAEIARFQAQGAYHADLNAHNILLTDAAVW
                     LIDFDRGELRNPARTWQQANLARLKRSLLKLGAARNGEDEFERGLWRPLLAAWEKEMA
                     S"
     gene            309..1079
                     /locus_tag="VFF05_00160"
     CDS             309..1079
                     /locus_tag="VFF05_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZW50246.1"
                     /translation="MNFALRFIGVGSAQAVELGSSGAVIERDGKPVLLIDCGPETLTT
                     YMDAYAEPPRAVYITHTHMDHVAGLERLFYRVYFDEALRGTVRLYAHAALIPHLQARV
                     ADYPQVVAEGGANFWDGFHAVPCSRGFWHDGLWFDVFPTRHHAPQTSFGIALRGSFVW
                     TGDTRPIPEMLAEFAPPPTVIAHDCALHGNPSHTGVDDLTREYDDATRSRMLLYHYGG
                     VADGLALAARGYRVAQRGERIALPPPAASAVLDPEGGA"
     gene            1076..>1259
                     /locus_tag="VFF05_00165"
     CDS             1076..>1259
                     /locus_tag="VFF05_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50247.1"
                     /translation="MNAPTIPPLPVPVRDAPVRDALRRPLHDLRISVIDRCNFRCTYC
                     MPEDQYALDHVFLGKDQ"
BASE COUNT          198 a          405 c          410 g          246 t
ORIGIN      
        1 ttcatgcaga accagctcga tgcggtcgta acctcgcggg tcggtgcgga aatcgcgcgc
       61 ttccaggcgc agggtgccta tcacgccgat ctgaatgcgc acaatatcct gctgacggac
      121 gccgcggttt ggctgatcga tttcgatcgc ggcgaattgc gcaatccggc gcgcacgtgg
      181 caacaggcga acctggcgcg actcaaacgc tcgctgctca aactcggagc ggcgcgaaat
      241 ggcgaagacg agttcgaacg cggcttgtgg cggcctctgc ttgccgcgtg ggaaaaggag
      301 atggcgtcgt gaactttgcg ttgcgtttta tcggtgtcgg ttccgcgcag gcagtggaac
      361 tgggttcgtc cggcgcggtg attgagcgcg acggcaagcc cgtcctgctc atcgactgcg
      421 gcccggaaac cctgacgacc tacatggatg cctacgccga gccgccgcgc gcggtttaca
      481 tcacgcacac gcacatggat cacgtcgcgg gcctggagcg cctgttctat cgcgtgtatt
      541 tcgacgaagc cctgcgcggc acggtgcgcc tgtatgcgca tgccgccctg attccgcacc
      601 tgcaggcgcg cgttgccgac tatccgcagg tggtcgccga gggcggcgct aatttctggg
      661 atggcttcca cgccgtgccg tgttcgcgcg gattctggca cgatggtttg tggttcgatg
      721 tgttcccgac gcggcatcac gcgccgcaga cttcattcgg catcgccttg cgcggatcgt
      781 tcgtctggac cggcgacacg cggccaattc cggaaatgct cgccgaattc gcaccgccgc
      841 cgaccgtgat cgcgcacgat tgtgcgctgc acggcaatcc ttcgcatacc ggcgtggatg
      901 atctgacgcg cgagtacgac gatgcgacgc gaagccggat gctgctctat cactacggcg
      961 gtgtcgccga tggcctggcg ctggcagcgc gcggctatcg cgtggcgcag cgcggcgagc
     1021 gcatcgcctt gccgccgcct gctgcgtccg cggtgctcga tcccgaggga ggcgcatgaa
     1081 cgctccgacg attcctccgc tgccggtgcc ggtgcgcgac gcgccggttc gcgatgcctt
     1141 gcggcgtcca ctgcacgact tgcgcatttc cgtgatcgac cgctgcaatt tccgctgtac
     1201 ttactgcatg ccggaagacc agtacgcact cgatcacgtg tttctcggca aggaccagc
//
LOCUS       DASRVO010000015         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_182176, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000015 DASRVO010000000
VERSION     DASRVO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182176"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFF05_00170"
     CDS             complement(<1..894)
                     /locus_tag="VFF05_00170"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HZW50248.1"
                     /translation="MTDGAAGNERQARALAQAMQIEAQTLRTGLRGPWAWFAPRLTAG
                     LRFGVDADLRRRLREPWPALVIGCGRDGALVTSWLRRASGGKTFAIQILDPRIDPKHF
                     DLVIAPRHDALEGANVVQTIGALNPVDDDWLAQGLARFPMLAQLPQPRTAVLIGGPRR
                     GLDMGEAWFEAFLARINALVTRDGGSALVSTSRRTPNAWRRLSREHLRADCVHFWNGS
                     EDGNNPYQGYLAAADRIAVTPDSVNMLSEACATGKPAFTLLPDSARGKLTGLHAELRT
                     QGWLHPLDAAVDTSGLPQPPPL"
     gene            1061..1645
                     /locus_tag="VFF05_00175"
     CDS             1061..1645
                     /locus_tag="VFF05_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZW50249.1"
                     /translation="MHVFARHLLIASLLAASASAFAAPQTFSIDPTHTQVSFTYDHFG
                     FSNPTGRLEDIKGTLVVDQADWAKSSVEVTMPLSGLHTGVPKLDEHLKTPDFFDAAKF
                     PDVTFKSTKVSKTGAQTLDIAGDLSVHGVTKPVTLHARINKIGENKMIGSQSAGFDAD
                     TTLKRSEFGLTKYVPMVSDEVHIHITLSADAAKK"
     gene            complement(1690..>2342)
                     /locus_tag="VFF05_00180"
     CDS             complement(1690..>2342)
                     /locus_tag="VFF05_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HZW50250.1"
                     /translation="ISVDGSDSNVSVDKNSALGALVAMGQNAQKASKQMEAAQKSGDA
                     NAQAQAAGQMLGAVLGGGSQVEALAPEAIKAFVPDTLAGMKRTSVSAERNGAMGMQVS
                     NATATYSDGQHTLHLEIADTGSAKGIMALAGAVGVESDNETDHGYDKTYKQDGRLVHE
                     QWDTQSKDGEFGIILGNRFSVKVSGNADSIDTLKAAVGSVKLADLEALKNQGVKSQ"
BASE COUNT          386 a          775 c          757 g          424 t
ORIGIN      
        1 cagcggtggc ggttgcggca atccggacgt atcaacagcg gcatccagcg gatgcagcca
       61 gccttgcgtg cgcagttcgg catgcaggcc ggtcaatttg ccacgtgcgg aatccggcag
      121 cagagtgaat gccggcttgc cggtggcgca ggcttcggac agcatgttca ccgaatcggg
      181 cgtgaccgcg atgcgatcgg cggcggcgag atagccctga tacgggttat tgccgtcttc
      241 cgatccgttc cagaagtgca cgcagtcggc gcgcagatgt tcgcgcgaaa ggcgccgcca
      301 cgcattcggc gtgcgccgcg acgtcgacac cagcgcgctg ccgccatcgc gcgtcacgag
      361 cgcgttgatg cgcgcgagga acgcctcgaa ccaggcctcg cccatgtcca gtccgcggcg
      421 cgggccgccg atcagcacgg cagttcgtgg ctgcggcaat tgcgccagca tggggaagcg
      481 cgcaagtccc tgggcgagcc agtcatcgtc caccggattg agtgcgccga tggtctggac
      541 cacgttcgcg ccttcgagcg catcgtggcg cggcgcgatg acgaggtcga aatgtttcgg
      601 atcgatgcgc ggatcgagga tctggatggc aaaggtcttg ccgccgctgg cgcggcgcag
      661 ccaacttgtg accagggcgc cgtcgcggcc gcagccgatg accagcgccg gccacggttc
      721 gcgcaggcgc cggcgcaaat ccgcatcgac gccgaaacgc aggccggccg tcaggcgcgg
      781 cgcgaaccac gcccagggtc cgcgcaggcc ggtgcgcagt gtctgcgctt ctatttgcat
      841 ggcctgcgcc aacgcgcgcg cctggcgctc gttgccggcg gcgccgtcgg tcacgatcca
      901 gcaagggggt gattggaaaa ccgcgtccga caccacagat gtcctgtgcg aggttcggcc
      961 atgatgccga gtccgcgttg tcgtcggcaa gtcgaaagca tccttaatgc cggttggatt
     1021 acactcggcc ggctcgtttc atcccatccc gcacaggaga atgcacgtgt tcgcacgcca
     1081 tctgttgatc gcatccctgc tcgccgcaag cgcttccgcc ttcgccgcgc cgcagacctt
     1141 cagcatcgat ccgacccata cccaggtatc gttcacgtac gaccatttcg gattttccaa
     1201 cccgaccggg cgcctggaag acatcaaggg cacgctggtc gtcgatcagg ccgattgggc
     1261 caagtcctcg gtcgaggtga ccatgccgtt gagcggcctg cacaccggcg tgccgaaact
     1321 cgacgagcat ctgaagacgc cggacttctt cgacgcggcg aaattccccg acgtcacttt
     1381 caagagcacc aaggtgagca agaccggcgc gcaaacgctc gacatcgccg gcgacctgag
     1441 cgtgcacggc gtgaccaagc cggtgacgct gcatgcccgc atcaacaaga tcggcgagaa
     1501 caagatgatc ggctcgcaaa gcgccggttt cgatgccgac accacgctca agcgttccga
     1561 gttcggcctg accaagtacg tgccgatggt cagcgacgag gtgcacatcc acatcacgct
     1621 gtcggcggac gctgccaaga aatgacgccc tgatgggcgc gcctctcccg cgcgcgggag
     1681 aggcggatat tactggctct tcacgccctg attcttcagg gcttccaggt cggcgagctt
     1741 gacactgccg accgcggcct tgagcgtatc gatgctgtcg gcgttgccgc tgaccttcac
     1801 gctgaagcgg ttgccgagga tgatgccgaa ttcgccgtcc ttgctttgcg tgtcccactg
     1861 ttcgtggacg agacggccat cctgcttgta ggtcttgtcg tagccgtggt cggtttcgtt
     1921 gtcggattcg acaccgacgg cgccggccag cgccatgatg cccttggcgc tgccggtgtc
     1981 cgcgatctcc agatgcaagg tgtgctggcc gtcggaataa gtcgcggtgg cgttggaaac
     2041 ctgcatgccc atcgcgccgt tgcgttcggc cgaaaccgag gtgcgcttca tgccggcgag
     2101 cgtgtcgggc acgaaagcct tgatcgcttc cggcgccagc gcctcgacct gcgaaccgcc
     2161 gccgagcacg gcgccgagca tttgcccggc cgcctgcgcc tgcgcgttgg cgtcgccgga
     2221 tttctgcgcg gcttccatct gcttgctcgc tttttgcgcg ttctggccca tcgcgaccag
     2281 cgcgccgagc gcgctgttct tgtccacgct cacgttgctg tcgctgccgt caacgctgat
     2341 gc
//
LOCUS       DASRVO010000016         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_26308, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000016 DASRVO010000000
VERSION     DASRVO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26308"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..858
                     /gene="miaA"
                     /locus_tag="VFF05_00185"
     CDS             13..858
                     /gene="miaA"
                     /locus_tag="VFF05_00185"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZW50251.1"
                     /translation="MGGEILACDSTQIYRHFDIGTAKVPRAEQHGIAHHLTDLVEPNE
                     VFTAGEYRRRAQEVLADLRRRGKVPVITAGTGLYLRALLEGLAEAPARSEELRERLRR
                     RAREGGGEHLHRILKRLDAEAGARIAPRDTQKVIRAIEMRILTGKPVGEIHRAGREAL
                     RGYRVIKIGLAPERAELYARINARVEAMIAAGWVGEVQELIGMGVAREAKAFQFIGYA
                     ELREHIEGRLALEAAMAKIEQVTRNFAKRQITWFRKEPGVRWLAGFGDDSKIVSEALE
                     IASVR"
BASE COUNT          211 a          301 c          392 g          163 t
ORIGIN      
        1 ctggcgcgcg aaatgggcgg cgaaattctg gcgtgcgatt cgacgcaaat ctaccggcat
       61 ttcgatatcg gcacggccaa agtgcctcgc gccgagcagc acggcatcgc acatcatctg
      121 acggatctcg tcgagccgaa cgaggtgttt acggccggag aataccggag gcgggcgcag
      181 gaagtactgg cagacctgcg gcggcgcggc aaggtgccgg tgatcacggc gggcacggga
      241 ctgtatttgc gcgcgctgct ggaagggctg gccgaagcgc cggcgcggtc agaggaattg
      301 cgggaacggc tgcggcggcg ggcgcgcgag ggcggcggcg agcacctgca ccgcattttg
      361 aagcggctcg acgcggaagc gggcgcgcgg attgcgccgc gcgacacgca gaaagtgatt
      421 cgcgcaatcg agatgcgcat cctgacgggc aagccagtcg gcgagatcca tcgcgcgggc
      481 cgcgaagcgt tgcgcggata ccgcgtcatc aagatcgggc tggcgccgga acgcgccgag
      541 ctgtacgcgc ggatcaacgc ccgcgtcgag gccatgatcg cggcgggatg ggtgggcgaa
      601 gtccaggaac tgatcgggat gggcgttgcg cgggaggcga aggcgttcca gttcataggc
      661 tacgcggaac tgcgggagca catcgaaggc cggctggcgc tggaggcggc gatggcgaaa
      721 atcgagcagg tcacgcgcaa tttcgccaag cggcaaatca cgtggttccg caaagaaccg
      781 ggcgtgcgct ggctcgcggg tttcggcgac gattcgaaga tcgtctccga agccttggag
      841 atcgcttcgg tgcgctgata cgggtaacgg ttcgagacaa ggcagcgccg cagcgcgcgg
      901 gcagaacttc aattcgattg gcaagcgcgg tatactttct gtagatgcaa tcgaaccact
      961 ccggcacggc cagacgtccg ttattatccg aatgaaatcc gtacatcccc tgaatcaacg
     1021 cgagcgagtg ttgctggtcg gcgtggcgct gaagcggccg agccacg
//
LOCUS       DASRVO010000017         1249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_52449, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000017 DASRVO010000000
VERSION     DASRVO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52449"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFF05_00190"
     CDS             complement(<1..595)
                     /locus_tag="VFF05_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HZW50252.1"
                     /translation="MHRQLLVIAPCLLVAACAGQPQRTREYAPDTAVTALYTRLDADT
                     GNYLAAREAGDATDQANAALDDLQTAASQCAATRGCDTARFFAAFDKLLRAPANAAPG
                     TADSADESPDASPEAGETSPVVATLPQAGRSVALLKGHKLGDIIAMNDPMKVAIEQWL
                     TQYRPNLMRAWENYQYLRHEMWPQYEKAGLPEALLFGM"
     gene            708..>1249
                     /locus_tag="VFF05_00195"
     CDS             708..>1249
                     /locus_tag="VFF05_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50253.1"
                     /translation="MVTPDANPQPATDPTPLHERWPQAFGYPLQMAALSTIVAIAVAH
                     VVVRLIPGIIGWIFDLIVWAGFFKYAFEVLRWSANGRSEAPEISFTVSDELGRYAVLL
                     LLLVEVALVLIAMWWGMLAALIVGIALMVAMPAMVTILALEEGLARALNPLVWLMIAS
                     RIGGHYFALVGFFCAALILQ"
BASE COUNT          190 a          407 c          405 g          247 t
ORIGIN      
        1 gcatgccgaa cagcaacgcc tcaggcagac cggccttctc gtactgtggc cacatctcat
       61 ggcgcaggta ttggtagttt tcccaggcgc gcatcagatt cgggcggtat tgagtcagcc
      121 attgctcgat cgccaccttc atcggatcgt tcatggcgat gatgtcgccg agcttgtggc
      181 ccttgagcag ggcgaccgag cgcccggcct gcggcagcgt cgcgaccacc ggggatgttt
      241 cgccggcctc gggggacgca tccggcgatt cgtcggcgct gtccgccgtg ccgggcgcgg
      301 cgttcgccgg tgcgcgcagc aacttgtcga acgcggcgaa gaatcgcgct gtgtcgcaac
      361 cgcgcgtcgc cgcacattgc gatgcggccg tttgcaaatc atccagcgcc gcgttggcct
      421 ggtccgtcgc atcgcccgct tcgcgcgcgg cgaggtaatt gccagtgtct gcgtccagcc
      481 gcgtgtacag cgcagtcacc gcggtatccg gcgcatattc cctcgtgcgc tgcggctgtc
      541 ctgcgcaggc ggcaacgagc aggcacgggg cgatgacgag aagctggcgg tgcatgatct
      601 gaactcctga cggtttccat cacgttaacc ggcatgacgc gccgcgacaa ccccgggcgg
      661 cgcatgccga tacaatgttg ccaacgcatt cttcggcacc atccgcaatg gtcactcccg
      721 acgccaaccc gcaacccgca accgacccga cgcccttgca cgaacgctgg ccgcaggcgt
      781 tcggctatcc gctgcagatg gccgcgttgt cgaccatcgt cgccatcgcg gtcgcccacg
      841 tcgtcgtgcg cctgatcccc ggcatcatcg gctggatatt cgatctgatc gtctgggccg
      901 gcttcttcaa gtacgcgttc gaggtattgc gctggagcgc gaacggccgc agcgaggcac
      961 cggagatcag tttcacggtg agcgatgaac tcggccgtta cgccgtgctg ctgttgttgc
     1021 tggtcgaagt cgcgctcgtc ctgatcgcga tgtggtgggg catgctggcc gcgctgatcg
     1081 tcggcatcgc gctgatggtc gcgatgccgg cgatggtcac gatcctggcg ctggaagaag
     1141 gcctggcgcg tgcgctcaat ccgctggtct ggctgatgat cgcctcgcgc atcggcggac
     1201 attatttcgc actggtcgga ttcttctgcg cggcgttgat cctgcaaag
//
LOCUS       DASRVO010000018         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_182225, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000018 DASRVO010000000
VERSION     DASRVO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182225"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1315
                     /locus_tag="VFF05_00200"
     CDS             <1..>1315
                     /locus_tag="VFF05_00200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZW50254.1"
                     /translation="VHLAVQTSLEREVALKVMAPALAADPAFSKRFLQEARMLASLAH
                     PNIVAVYDVGVTPAQLHYFSMQYLPGGDFTSRVNRGIEERELVLTLAGVARALGYAHQ
                     RGYVHRDVAPGNVLYDANGNPVLTDFGIALATVTGGHITSSGFSVGTSHYMSPEQARG
                     GDVDARSDIYSLGVLAYFGLARKPPYDGADGFAVAYAHVFEPIPRLPAEHAHWQSLID
                     RALAKDPKDRYADTEQFLDALAGVAPHYAALFREDAAAPATVPAAERATVTMKPVREE
                     PATRPLARKVEADEAATMRSMRPATTSAPTSVEASASVSAPPRAWLRWWPVAIVVAGI
                     ALIGFALFARRGTPPPVPAPVTAAPAAAAPAESAVPAIAENASATAPVAAAVAPPTTA
                     MDSIDAAETQSAGTPPQAAEEFPTVVDPLVEALRLGQLDLTQQRLT"
BASE COUNT          208 a          466 c          428 g          213 t
ORIGIN      
        1 gtgcatctgg cggtgcagac ctcgctcgag cgcgaagtcg cgctcaaggt gatggcgccc
       61 gccctcgcag ccgacccggc gttttccaag cgctttctgc aggaagcgcg catgctcgcc
      121 tcgcttgcgc acccgaacat cgttgccgtg tacgacgttg gcgtgacccc ggcgcaacta
      181 cattatttct cgatgcaata cctgccgggc ggcgacttta cctcgcgcgt caaccgtggc
      241 atcgaagaac gtgaactggt actgaccctg gctggcgtgg cgcgcgcctt gggctatgcg
      301 caccagcgcg gctacgtgca tcgcgatgtc gcgccgggca acgtcctcta cgacgcgaac
      361 ggcaatccgg tactgaccga tttcggcatc gccttggcca cggtcaccgg tggccacatc
      421 acgagcagtg gattttcggt cggcaccagc cattacatga gcccggaaca ggcgcgtggc
      481 ggcgacgtgg atgcgcgctc ggacatttac agccttggtg tgctcgccta tttcgggctt
      541 gcgcgcaagc cgccctacga cggcgccgat gggttcgccg tcgcctatgc gcatgtgttc
      601 gagccgattc cgcgccttcc cgccgagcat gcgcactggc agagtctgat cgaccgcgcg
      661 ctggccaagg atccaaaaga ccgctacgcc gacaccgaac aattcctcga cgcgctggcc
      721 ggcgttgcgc cgcactatgc cgcattgttc cgtgaggacg ccgccgcacc ggcaaccgtg
      781 ccggcagccg aacgcgccac ggtcacgatg aagcccgtgc gcgaggagcc ggccacgcgg
      841 ccgctcgcgc gcaaggtcga agcggacgaa gcggcgacca tgcgcagcat gcgtccggcc
      901 acgacgtccg ctccgacaag cgtcgaagcc tctgcgtctg tgtctgcgcc gccgcgcgct
      961 tggctgcgct ggtggccggt cgcgatcgtt gtcgctggaa tcgccttgat cggattcgcc
     1021 ctgttcgcgc gccgcggcac tccaccaccg gtgccagcac ctgttaccgc cgcgccggcg
     1081 gccgcggccc cggctgaatc cgcggtgccg gccatcgcgg aaaacgcttc ggcgacggca
     1141 cccgtggcgg cggccgtggc accgccgacg acggcgatgg acagcatcga tgccgccgag
     1201 acgcagagcg caggcacgcc gccacaggcg gccgaggaat ttccgaccgt ggtcgatccg
     1261 ctcgtggaag cgctgcgcct gggtcaactg gacttgaccc agcagcggct cacca
//
LOCUS       DASRVO010000019         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_52521, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000019 DASRVO010000000
VERSION     DASRVO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52521"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFF05_00205"
     CDS             complement(<1..272)
                     /locus_tag="VFF05_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZW50255.1"
                     /translation="MALTPVGAHKMAADAQPDMPPWVRGEAPLRVRDFVFAAAIVAGA
                     SCLGFFWVMHLWPSKIAMLFLAAVLLTGYRFGMWPAISAAFLAFLS"
     gene            complement(284..853)
                     /locus_tag="VFF05_00210"
     CDS             complement(284..853)
                     /locus_tag="VFF05_00210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZW50256.1"
                     /translation="MTTYKVGYFVGSLARHSINRMLAKALQRLAPPALSMFEIPFKDL
                     PLYSYDYDADFPPVAREFKDKIAQADALLFVTPEYNRSIPGGLKNAIDCASRPWGTNS
                     FARKPSAVIGTSPGSIGTAIAQQSLRGVLCFCNSPLMNAVEAYIQFTPGLITETGEVT
                     NDKTAEFLRNYMTEFHAFIARVKTAMSTA"
BASE COUNT          199 a          329 c          337 g          188 t
ORIGIN      
        1 tacgacagga acgccaggaa cgccgcagat atcgccggcc acatgccgaa gcggtatccg
       61 gtcaacagga ccgcggccag gaaaagcatc gcgatcttgc tcggccacaa gtgcatgacc
      121 cagaagaagc ccaagcacga cgcgccggcg acgatcgccg ctgcgaagac gaagtcgcgc
      181 acgcgcaacg gcgcttcgcc gcgcacccat ggcggcatgt cgggctgcgc gtccgccgcc
      241 atcttgtggg cgccgaccgg agtcaaggcc atgcgcgtgg cgttcaggcc gtcgacatgg
      301 ccgtcttcac gcgcgcgatg aaggcgtgga attccgtcat gtagttgcgc aggaactccg
      361 cggtcttgtc gttggtgacc tcgcccgttt cggtgatcaa acccggcgtg aactggatgt
      421 aggcttcgac cgcgttcatc agcggcgaat tgcagaagca caacacgccg cgcaggctct
      481 gttgcgcgat cgcggtgccg attgagccgg gcgaagtgcc gatcaccgcg gacggcttgc
      541 gcgcgaacga attcgtgccc cacggccggc tcgcgcagtc gatcgcgttc ttcaggccac
      601 cgggtatcga gcggttgtat tccggggtca cgaagagcag ggcgtcggct tgcgcgatct
      661 tgtccttgaa ctcgcgcgcg accggcggaa agtccgcgtc gtagtcgtag ctgtacagcg
      721 gcagatcctt gaacggaatc tcgaacatcg acagcgcggg cggcgccaag cgttgcaacg
      781 ccttcgccag catgcgattg atcgaatgtc tggccaggct gccgacgaag tagcccactt
      841 tgtaagtcgt catgcgcggt ctccggttga ggacggcgcg gacattctag acccgcatca
      901 acgccgaagc gtcacgccat tgtttgccgc gctcggaggg attcagccga actgagacga
      961 atcgagtagc cacttggctg gtgagttctc ttaatccatg gcactgcccg cggcctgcgc
     1021 gcgccatttg tcatcttgac gacgaatcct tga
//
LOCUS       DASRVO010000020         3345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_286260, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000020 DASRVO010000000
VERSION     DASRVO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286260"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFF05_00215"
     CDS             complement(<1..323)
                     /locus_tag="VFF05_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZW50257.1"
                     /translation="MAKITKSFQYGNHQVTLETGEIARQASGAVMVSIDGTVVLVTAV
                     ANAKAKEGQDFFPLTVDYVEKFYSAGRIPGGFFKREGRPTEKETLTSRLIDRPVRPLF
                     PEEYK"
     gene            complement(447..716)
                     /gene="rpsO"
                     /locus_tag="VFF05_00220"
     CDS             complement(447..716)
                     /gene="rpsO"
                     /locus_tag="VFF05_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZW50258.1"
                     /translation="MSLTVEQTSKVIADYRRAANDTGSPEVQVALLSARIEHLSKHFA
                     AHDKDHHSRRGLLKMVNQRRSLLAYLKKSDAGRYQTLIERLGLRR"
     gene            complement(856..1770)
                     /gene="truB"
                     /locus_tag="VFF05_00225"
     CDS             complement(856..1770)
                     /gene="truB"
                     /locus_tag="VFF05_00225"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZW50259.1"
                     /translation="MKPARRAVTGILLLDKPAGISSNRALQQARRLFNADKAGHTGSL
                     DPLATGLLPICFGEATKIAGYLLGSRKAYAAQCRLGTTTTTDDAEGEVVRRLPVPELH
                     DKTIEAALATLRGRIVQTPPAYSAIKQGGVPLYKRARRGEDVVVPSRDVEVDRFDLQG
                     RAGDTLSLHVECGSGTYVRSLARDLGERLGCGAHLTALRRLWVEPFRDPVMHTLESLE
                     QIAADGGLAALDRLLLPIEAGLGALPALHLDAEQARRLRHGQRLSLPGQGPLPLCRAM
                     NAQGRLVALVRLDVEGTVAIVRGFAAPD"
     gene            complement(1767..2111)
                     /gene="rbfA"
                     /locus_tag="VFF05_00230"
     CDS             complement(1767..2111)
                     /gene="rbfA"
                     /locus_tag="VFF05_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZW50260.1"
                     /translation="MPSHSFTRSDRLAAELRRLVGTLVHDAVREHALPSMSVSDVECS
                     KDFDVATVYVTALLPDTAAEGVKALKAMAKDLRTQLSKIMRVRRVPELRFKYDDSVDR
                     GERIDALLRGEK"
     gene            complement(2111..>3345)
                     /gene="infB"
                     /locus_tag="VFF05_00235"
     CDS             complement(2111..>3345)
                     /gene="infB"
                     /locus_tag="VFF05_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZW50261.1"
                     /translation="IEAIQHAKAARVPLIVAVNKIDKPEANPDRVKQDLIQHEVVSED
                     FGGDVQFVPVSAKTGQGIDQLLDAILLQAEVMDLKAVVDGLATGAVVEASLDKGRGPV
                     ATVLVQQGTLKKGDFLVCGVEYGRVRAMFDESGQPVESAGPSIPVQLLGLSGVPNAGD
                     DFVVVQDERLAKQVAAERQAKLRESRLTKQSVRLEDVISQMGQGDEQAVLNLVVKTDV
                     QGSAEALRDSLTAIGNDRVKVNVIASGVGGITESDATLAVASKAIVIGFNVRADATAR
                     KVLETNGIDLRYFSIIYDVIDQVKQAATGLLGMEIREEIIGTAQVRDVFRSSKFGAIA
                     GCMVIEGVVKRNKPIRVLRDNTVIYQGELESLRRFKENVDEVRNGMECGIGVKQYNDV
                     KPGDQIECYERIEVARTL"
BASE COUNT          565 a         1063 c         1077 g          640 t
ORIGIN      
        1 ttcttgtact cttccggaaa cagcggacgc accggacggt cgatcaggcg cgaggtcagc
       61 gtttcctttt cggtcggacg cccttcgcgc ttgaagaagc cgccgggaat gcgtcccgcg
      121 gagtagaact tctcgacgta gtcgacggtg agcgggaaga aatcctggcc ttccttcgcc
      181 ttggcgttgg cgaccgcggt gacgaggacg acggtgccgt cgatcgacac catgacagcg
      241 ccggacgcct ggcgggcgat ttcgcccgtc tccagcgtga cctgatgatt tccgtactgg
      301 aacgacttgg taattttggc catggtattt tttgccttcg gttatttcgt cactccggcc
      361 catgccggag tcggtctttc gtcttgcgat ttttcgaacc acatacaaaa caaatccccg
      421 ccgaagcggg gatcgtgatg caccgattag cggcgcaggc ccagacgctc gatcagggtc
      481 tggtagcggc cggcgtcgga tttcttcagg taggccagca gactacggcg ctggttgacc
      541 atcttgagca gaccgcggcg cgagtggtga tccttgtcgt gcgcggcgaa gtgcttggaa
      601 agatgttcga tgcgtgcgga aagcagggcc acctggactt ccggcgagcc ggtatcgttg
      661 gcggcgcggc gatagtccgc gatgaccttg ctggtttgtt cgacagttag cgacatgttg
      721 ggtacctcgt tgatgaaacg tacggcgcgg ttcggcttgt cgggagcgcg atccgcgtta
      781 gggactgcgg gaaatccgca gggcttggaa aagccgctta ttgtaacccg atttggcggc
      841 gcgcggaaac cgcgctcagt ccggcgccgc gaacccgcgc acgatggcaa ccgtaccctc
      901 cacatccagc cgcaccagtg cgaccaacct gccctgcgcg ttcattgccc ggcacagagg
      961 cagcggtcct tggccgggca ggctcaggcg ctggccgtgg cgcaggcgcc tggcctgttc
     1021 ggcatcgaga tgcagggccg gcaacgcgcc gaggcccgcc tcgatcggca gcagcaggcg
     1081 gtcaagtgcc gccagtccac cgtcggcggc gatttgttca aggctttcca gcgtgtgcat
     1141 gaccggatcg cggaaaggct ccacccacag tcgccgcagt gcggtcagat gcgcgccgca
     1201 gcccaggcgt tcgccgaggt cgcgcgcgag gctgcgcacg taggtgccgg agccgcattc
     1261 gacatgcaag ctcagtgtat cgccggcgcg gccttgcaga tcgaaacgat cgacttcgac
     1321 atcgcgcgac ggaaccacaa cgtcttcgcc gcgccgcgcg cgcttgtaca acggcacgcc
     1381 gccctgcttg atcgccgaat acgcgggcgg cgtctgcacg atgcggccgc gcaaggtcgc
     1441 caatgccgct tcgatggttt tgtcgtgaag ttcaggaaca ggcaacctgc ggacgacttc
     1501 gccttcggca tcgtcggtcg tcgtggtggt gccgagccgg cattgcgcgg cgtaggcctt
     1561 gcgcgagccg agcaagtaac cggcgatttt cgtcgcctcg ccgaagcaga tcggcaacag
     1621 tccggtggcg agcggatcga ggctgccggt gtgtccggcc ttgtcggcgt tgaaaaggcg
     1681 ccttgcctgt tgcaacgcgc ggttggagct gattcctgcg ggcttgtcga ggagcaggat
     1741 gccggtaacg gcccgccggg cgggtttcat ttttccccgc gcagcaaggc gtcgatgcgc
     1801 tcgccgcgat ccaccgaatc gtcgtacttg aaacgcagct cgggaacgcg gcgcacgcgc
     1861 atgatcttcg acaactgcgt gcgtaaatcc ttggccatgg ccttgagcgc cttcacgcct
     1921 tcggcagccg tatccggaag cagggccgtg acatacaccg tggccacatc gaaatccttg
     1981 ctgcactcga catccgacac gctcatcgac ggcaacgcat gctcgcgcac cgcgtcgtgc
     2041 acgagcgtgc cgacaaggcg gcgcaactcg gcggcaaggc ggtcggagcg ggtgaaggaa
     2101 tgcgaaggca ttacaaggtt ctggctactt caatgcgctc gtagcactcg atctggtcgc
     2161 cgggcttgac gtcgttgtac tgcttcacac cgatgccgca ttccatgccg ttgcgcactt
     2221 cgtcgacgtt ttccttgaag cgacgcagcg actcgagttc gccctggtag atcacggtgt
     2281 tgtcgcgcag cacgcggatc ggcttgttgc gcttgaccac gccttcgatg accatgcagc
     2341 cagcgatggc gccgaatttc gacgaacgga acacatcgcg cacctgggcg gtgccgatga
     2401 tctcctcgcg gatctccatg ccgagcaggc cggtagcggc ctgtttcacc tggtcgatca
     2461 cgtcgtagat gatcgagaag tagcgcaggt cgatgccgtt ggtttccagc accttgcgcg
     2521 cggtcgcgtc cgcgcgcacg ttgaagccga tgacgatagc cttcgaggcc accgcaagcg
     2581 tggcatcgga ttcggtgatg ccgccgacgc cagaggcgat gacgttgacc ttgaccctgt
     2641 cgttgccgat cgcggtgagc gaatcgcgca acgcttcggc ggaaccttgc acgtcggtct
     2701 tgacgacgag gttgagcacg gcctgctcgt cgccctggcc catctgcgaa atcacgtctt
     2761 ccaggcgcac cgactgcttg gtcaggcgcg actcgcgcag cttggcctgg cgctcggcgg
     2821 cgacctgctt ggccaggcgc tcatcctgga ccacgacgaa atcgtcgccg gcattcggca
     2881 cgccggaaag tccgagcaac tgcaccggga tcgagggacc ggcgctttcc accggctggc
     2941 cggattcgtc gaacatcgcg cgcacacgac cgtattccac gccgcacacg aggaaatcgc
     3001 ctttcttcag cgtgccctgt tgcaccagca cggtcgcaac cgggccgcgg cccttgtcca
     3061 ggctcgcttc gacgacggcg ccggtggcga ggccatccac gaccgccttg agatccatca
     3121 cctcggcctg cagcaggatc gcatcgagca gttgatcgat gccctgcccg gttttcgccg
     3181 agaccggcac gaattgcacg tcgccgccga aatcctcgga cacgacttcg tgctggatca
     3241 ggtcctgctt gacgcggtcc ggattcgcct cgggcttgtc gatcttgttc accgcgacga
     3301 tcagcggcac gcgcgcggcc ttggcgtgct ggatcgcctc gatcg
//
LOCUS       DASRVO010000021         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_208359, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000021 DASRVO010000000
VERSION     DASRVO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208359"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VFF05_00240"
     CDS             <1..1194
                     /locus_tag="VFF05_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZW50262.1"
                     /translation="ATLAARWREVDTELRALSGGQDHSERIDMLEHQLDELDRHALTP
                     VEIDMLNEMHKRLANAGQLLQGSESVAELLDGDSEFAALRALTRAQNELSRLAGLDAA
                     LAPARELLDAAAIQLGEAHSAIGRYRDGLELDPDRFAEAEAQIAKLHELSRKHRVPAA
                     ELKAHAEKLRAELEALHGAGERLATLQRERDGIRRDYATAAATLSQKRMAAAQRLDKE
                     ITALMAELGMAGGRFETQLTIADDTTPNAQGVERAEFLVAANPGQSPRPLRKVASGGE
                     LSRISLAIEVATLGMDEVGTMVFDEVDSGIGGAVAEVVGRKLRRLGGKRQVLCVTHLP
                     QVAAQAHAHLRVVKSSAAQSTRIAIETLGDSTRRDEIARMLGGVKITSQTLAHAQQML
                     ASAQA"
     gene            complement(1208..1621)
                     /gene="fur"
                     /locus_tag="VFF05_00245"
     CDS             complement(1208..1621)
                     /gene="fur"
                     /locus_tag="VFF05_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HZW50263.1"
                     /translation="METEELRKAGLKVTQPRMRILEILETSSGKHMTAEDIYRELIQH
                     HSDVGLATVYRVLTQFEAAGLVLKHNFEGGQAIYEIDRGKHHDHMVDIDTGKVIEFTS
                     EAIEKLQHEIAERHGYTIEDHSLVLYVRARKKKTP"
     gene            1744..2121
                     /gene="bamE"
                     /locus_tag="VFF05_00250"
     CDS             1744..2121
                     /gene="bamE"
                     /locus_tag="VFF05_00250"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HZW50264.1"
                     /translation="MAITACSPIYKLDIQQGNLFNKNLVDSLKPGMSKRQVTLVMGSP
                     SIVSPFDQNRWDYVSTLRKGNGRMATKDLTLYFENDSLAKIEGDYFAEDPVQLVKEAT
                     KYKRQYPDEKRPDDDKKKQQQGS"
     gene            complement(2118..>2356)
                     /locus_tag="VFF05_00255"
     CDS             complement(2118..>2356)
                     /locus_tag="VFF05_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HZW50265.1"
                     /translation="DPLRQFLRAVEVAAGSTVADAIAASGILAQLPGFVPAGIGIFGR
                     AVAPDTRLRDGDRVELYRALKIDPKQARRNRARD"
BASE COUNT          467 a          752 c          737 g          400 t
ORIGIN      
        1 gccacactgg ccgcgcgctg gcgcgaggtc gatacagaac tccgcgcgtt gtccggtggg
       61 caggaccatt ccgaacgcat cgacatgctc gaacaccagc tcgacgagct cgaccggcat
      121 gcgctgacgc cggtggaaat cgatatgttg aacgaaatgc acaaacgcct cgccaatgcc
      181 gggcaactgc tgcaaggcag cgagtccgtg gccgaattgc tcgacggcga cagcgaattc
      241 gcggcgctgc gtgcgttgac gcgagcgcag aacgaattgt cccggctggc cggactcgat
      301 gccgccctcg cgcccgcgcg cgagctgctc gatgccgcgg caatccagct cggcgaagcg
      361 cattccgcca tcggccgcta tcgcgatggc ctggaactgg atccggatcg cttcgccgaa
      421 gccgaagcgc agatcgccaa gctgcacgag ctgtcgcgca aacaccgcgt ccccgccgcc
      481 gaactgaagg cgcatgcgga aaaactgcgc gcggaattgg aagctctgca cggcgcaggc
      541 gagcgtttgg cgacgctgca gcgcgaacgc gacggcatcc gccgcgatta cgcgacggcg
      601 gcggcgacat tgtcgcaaaa gcgcatggca gcggcgcagc gactggacaa ggaaatcacg
      661 gcgctgatgg cggaacttgg catggccggc ggccgcttcg agacgcagct caccatcgcg
      721 gacgacacca ccccgaatgc gcagggcgta gaacgcgcgg aattcctggt cgccgccaat
      781 ccgggacagt cgccgcggcc gttgcgcaag gtcgcctccg gtggcgagtt gtcgcggatc
      841 agcctggcca tcgaagtggc aacgctgggc atggacgaag tcggcaccat ggtgttcgac
      901 gaagtcgatt ccggcatcgg cggcgcagtc gccgaagtcg tcgggcggaa acttcgccgg
      961 ctcggcggca aacgccaggt cctgtgcgtg acgcatctgc cgcaggtcgc tgcgcaggca
     1021 cacgcgcacc tgcgcgtggt caaatccagt gctgcgcaat ccacgcgcat cgcgatcgag
     1081 acgctgggtg attccacgcg ccgcgacgag atcgcgcgca tgctcggcgg agtaaagatc
     1141 acgagtcaga cgctcgcgca cgcgcaacag atgctcgcca gcgcgcaggc gtgacagaca
     1201 gaccgggcta cggcgttttc ttcttccttg cgcgcacgta cagcacgagg ctgtgatcct
     1261 cgatcgtgta gccgtggcgt tcggcgattt cgtgctgcaa cttctcgatc gcctcgctgg
     1321 tgaattcgat caccttgccg gtatcgatgt cgaccatgtg gtcgtgatgc ttgccgcgat
     1381 cgatctcgta gatggcctgg ccgccctcga aattgtgctt gagcaccagg ccggcggcct
     1441 cgaactgggt cagtacgcga tatacggtgg ccaagcctac gtcgctgtga tgctggatca
     1501 gctcacggta aatatcttcc gccgtcatat gcttgccgga agaagtttcc agaatttcga
     1561 ggatgcgcat gcgtggctgg gtgaccttca atccagcctt gcgcagttct tcggtttcca
     1621 tccagccctc gcacgcgata acgagcagga aatgcgctct agtgtatcat cgcccgcttt
     1681 ccgcccgctg gtatgcaccg catgacgacg tttttccgca ccgccgctgt cgcgctttgt
     1741 gtcatggcca tcacggcatg cagcccgatc tacaagctcg acatccagca gggcaacctg
     1801 ttcaacaaga acctggtcga ctcgctcaag cctggcatga gcaagcgtca ggtgaccctc
     1861 gtcatgggtt cgccgtccat cgtcagcccg ttcgaccaga accgctggga ctacgtctcg
     1921 accttgcgca agggcaacgg tcgcatggcg accaaagacc tgaccctgta cttcgaaaac
     1981 gactcgctgg cgaaaatcga aggcgattac tttgccgagg atccggtcca actggtcaag
     2041 gaagcgacca agtacaagcg ccagtatccg gacgaaaaac gcccggacga cgacaagaag
     2101 aaacaacagc agggcagcta gtctcgcgcg cgattgcgac gcgcctgctt cggatcgatt
     2161 ttcaaggcac ggtaaagctc gacgcgatcg ccgtcgcgca ggcgcgtatc gggcgcgacc
     2221 gcacggccga agatgccgat tccggccgga acgaatcccg gtagttgcgc gagaattcca
     2281 ctggccgcga tggcatcggc gacggtcgat cccgccgcca cttccacggc gcgcagaaat
     2341 tgccgtagcg gatcgg
//
LOCUS       DASRVO010000022         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_364224, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000022 DASRVO010000000
VERSION     DASRVO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364224"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFF05_00260"
     CDS             <1..395
                     /locus_tag="VFF05_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZW50266.1"
                     /translation="EIRELEARVRAMIRRDRRQVSAETLHVADLVYDTASVRVSRAGQ
                     DLQVSPIGLKLLSILMRESPRVVSRADIEREVWGDLLPDSDTLRSHLYNLRKIIDKPF
                     QRPLLHTIHSAGYRLADLDAEVAASKRA"
     gene            392..1708
                     /locus_tag="VFF05_00265"
     CDS             392..1708
                     /locus_tag="VFF05_00265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZW50267.1"
                     /translation="MKRAPRTGIRRKIWAVYVLQVAAISLATILGVYGAATVLEDVLI
                     KRALAGESAFYWHRWDRNHAAPVPETNNMTGYLLASGADPAALPQALRLLAPGYHRVQ
                     NGGSFALVHVSDGPPGRLFLVFNQEQVGHLAFLFGFLPLILVLLIIYITTWLTYRASR
                     RALSPVIALAKVVRDWDPKNPNLAALDPAQLSNDPDGDVETLARALHGYASRIEEFVA
                     RERDFTRDASHEMRSPLTVIKLAADVLVEEDVSAFAQRALQRIRRSVRDMEAMLEAFL
                     LLARESDTGLPQEEFVVNDAVREEAERAQPLLEGKPVTLELDEQARFALRAPPRVFAA
                     MIGNLIRNACLYTESGSVRVSVGEDSVSVADSGIGMSAEEIARTHQPYFRAGRSTSAG
                     HGVGLSIVRRLSERFGWPVDLRSEVGAGTVATIRFPQRLPAPPSAS"
     gene            1790..>2020
                     /locus_tag="VFF05_00270"
     CDS             1790..>2020
                     /locus_tag="VFF05_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZW50268.1"
                     /translation="MSQAADRAVLVIHGGAGVIKREMNPGTQKAIRAALTKALEQGYA
                     QLKAGKPALDAVTAAIVVLEDDPHFNAGKGAVF"
BASE COUNT          333 a          687 c          658 g          342 t
ORIGIN      
        1 tcgagatccg cgagctcgaa gcgcgcgtgc gcgccatgat ccggcgcgac cgccgccagg
       61 tcagcgcgga aaccttgcac gtggccgatc tcgtatacga caccgccagc gtgcgcgtaa
      121 gccgcgccgg gcaggacttg caggtgtctc cgatcggttt gaaattgttg tcgatcctga
      181 tgcgcgaatc gccgcgcgtg gtcagccgcg ccgacatcga gcgcgaggtc tggggcgacc
      241 tgttgcccga ttcggatacg ctgcgcagcc atttgtataa tttgcgcaaa attatagaca
      301 agcccttcca gcgtccgctg ctgcacacca tccatagcgc cggctaccgc cttgccgacc
      361 tggacgccga ggtggcggcc agtaaacgcg catgaaacgt gcgccgcgca ccggaatccg
      421 gcgcaagatc tgggcggttt acgtcctgca ggtggcggcg atcagccttg ccaccatcct
      481 cggtgtttac ggcgcggcca ccgtgctcga agacgtgctg atcaagcgcg cgcttgccgg
      541 cgagtcggcg ttctattggc accgctggga tcgcaaccat gccgcgccgg tgccggaaac
      601 caacaacatg acaggctacc tgctggcgtc cggcgccgac ccggccgcgt tgccgcaggc
      661 gctgcgccta ctcgcgcccg gctaccaccg cgtccagaac ggcggcagtt tcgcgctcgt
      721 gcatgtctcc gacggtccgc cggggcgcct gtttctcgtc ttcaaccagg aacaggtcgg
      781 acacctcgcc ttcctgttcg gattcctgcc gctgatcctg gtgctgctga tcatctacat
      841 caccacctgg cttacttacc gcgcgtcgcg gcgtgcgctg tcgccggtga tcgcgctggc
      901 caaggtcgtg cgcgactggg atccgaagaa cccgaacctg gccgcactcg atccggcgca
      961 gctttccaat gacccggacg gcgacgtcga gacgctcgcg cgcgcgctgc acggctacgc
     1021 cagccgcatc gaggaattcg tcgcgcgcga gcgcgatttc acccgcgacg ccagccacga
     1081 gatgcgcagc ccgctgaccg tgatcaagct cgccgccgac gtgctcgtcg aggaagacgt
     1141 ctcggcgttc gcgcaacgcg cgctgcaacg catccgccga tcggtgcgcg acatggaggc
     1201 gatgctcgag gctttcctgc ttctcgcgcg cgaatccgac accggcctgc cgcaggagga
     1261 attcgtggtc aacgacgcgg tgcgcgagga agccgaacgc gcccaacccc tgctggaagg
     1321 caagcccgtg accctggaac tggacgagca ggcgcgtttt gccctgcgcg cgccgccgcg
     1381 cgtgttcgcg gcgatgatcg gaaatctgat ccgcaacgcc tgcctgtaca ccgagtccgg
     1441 cagtgtgcgc gtgagcgtcg gcgaagacag cgtcagcgtc gccgacagcg gcatcggcat
     1501 gagcgcagaa gaaatcgcgc ggacgcatca gccgtacttc cgcgctggcc gcagcaccag
     1561 cgccggacac ggcgtgggcc tgagcatcgt gcgccgcttg tccgagcgtt ttggctggcc
     1621 ggtggacctg cgcagcgagg tcggtgccgg taccgttgcg acgatccggt ttccgcaacg
     1681 cttgccagcg ccgccgtcgg cgtcataatc ggcgcatgct cccgcggagg ccgcaccgat
     1741 gtttcgcaaa ttcgctgcca tcgttttgtc cgtcttcggg gtctgtgcca tgagtcaggc
     1801 cgcggatcgg gccgtgctgg tcatccacgg cggggccggc gtgatcaagc gcgaaatgaa
     1861 tccgggcacg cagaaggcga tccgcgccgc gctgaccaaa gcgcttgaac aaggttacgc
     1921 gcaactcaag gccggcaagc cggcgctcga cgccgtgacc gcggcgatcg tcgtgcttga
     1981 agacgatccg catttcaacg ccggcaaggg tgcggtgttc
//
LOCUS       DASRVO010000023         1369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_312296, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000023 DASRVO010000000
VERSION     DASRVO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312296"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFF05_00275"
     CDS             <1..542
                     /locus_tag="VFF05_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HZW50269.1"
                     /translation="GVRMQAVHPGTPKADCRLEFCEATDLSGSEWSLACDGFSVYVDA
                     QSMPYLEEALIDYAAEATGGQLIVRAPKLKGTPPSADADLAERVRYVLETEVNPQIAA
                     HGGRATLVEVTAEKAVMLRLGGGCHGCGMADVTLKQGIERTLRKHFPEISAVRDATDH
                     ASGHNPYYRGHDGRSAVHE"
     gene            complement(544..897)
                     /locus_tag="VFF05_00280"
     CDS             complement(544..897)
                     /locus_tag="VFF05_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZW50270.1"
                     /translation="MNFIRLTALAAGLIAINAFAAEAGGAPGASAADLAKTCAACHGA
                     DGNSTAPIYPRLAGQYHDYLARALQEYKSGKRQNAIMAGFAKTLSDADIDKLSRYFAA
                     MPSKLGDLHDRVQGD"
     gene            complement(907..1251)
                     /locus_tag="VFF05_00285"
     CDS             complement(907..1251)
                     /locus_tag="VFF05_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZW50271.1"
                     /translation="MQKIASACRTALFATALFAVPALAADTGKGDPQTGKTLVYTCHG
                     CHGVTGYENAYPKYRVPRIAGQNYDYIVAALTEYKKGERAHPTMRAQGESLSDADIRN
                     IAAYLSSLSPSQ"
BASE COUNT          240 a          399 c          462 g          268 t
ORIGIN      
        1 tcggtgtgcg catgcaggcc gtgcatcccg gtacgccgaa ggcggattgc cggctggaat
       61 tctgcgaggc taccgatttg tcgggcagtg aatggtcgct ggcctgcgac ggcttcagcg
      121 tgtacgtgga cgcgcagagc atgccgtatc tggaagaagc gctgatcgat tacgcggcag
      181 aagccaccgg tggccagttg atcgtgcgcg cacctaaatt gaaaggcact ccgccgtcgg
      241 ccgatgccga cctggccgag cgcgtgcgtt atgtgctgga aacggaagtc aatccgcaga
      301 tcgccgcgca tggcggccgt gcgacgctgg tcgaggtgac cgcggaaaag gccgtgatgc
      361 tgcgcctggg cggcggctgt catggctgcg gcatggccga cgtgaccttg aagcagggca
      421 tcgaacgcac cctgcgcaag cactttccgg agatttcagc cgttcgcgac gcgaccgatc
      481 atgccagcgg gcacaatccg tactatcgcg gccatgacgg ccgcagcgcc gtgcacgaat
      541 gaatcaatcg ccctgtacgc gatcgtgcag gtcgccgagc ttgctcggca tcgcggcgaa
      601 gtagcgcgat agcttgtcga tatcggcatc gctcagggtt ttggcaaagc ccgccatgat
      661 cgcgttctgg cgcttgcccg acttgtactc ctgcaatgca cgcgcaagat agtcgtggta
      721 ttggccggcg aggcgcggat agatcggtgc tgtggaattg ccgtccgcac cgtggcaagc
      781 cgcacacgtt ttggcgagat cggccgcgct ggcgccgggc gcaccgccgg cctccgccgc
      841 gaaagcgttg atcgcgatca atccggccgc aagcgccgtg agtcgaatga aattcattgg
      901 ctttccttat tggctcgggc tcaggctgga caggtacgcg gcgatgttgc ggatgtccgc
      961 gtcggacagg ctctcgccct gcgcgcgcat ggtcgggtgc gcgcgttcgc ctttcttgta
     1021 ttcggtcagc gcggccacga tgtagtcata attctggccc gcaatgcgcg gcacgcggta
     1081 tttcggatac gcattttcgt agccggtaac accgtggcag ccgtggcagg tatataccag
     1141 ggtcttgccg gtctgcggat cgcccttgcc ggtatcggcg gcgagcgcgg gaacggcgaa
     1201 cagcgcggtt gcgaacaaag ctgtgcggca ggcggaggcg attttttgca tggtgggtcg
     1261 tgcaatccgt gactgggcga aaaacccgcg aagtataacg tggcgaatgc gcgcatcgct
     1321 acccgcgtgg ctcacgcatg caaatttgtc gttgcgattc gaattccac
//
LOCUS       DASRVO010000024         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_26494, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000024 DASRVO010000000
VERSION     DASRVO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26494"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3076)
                     /locus_tag="VFF05_00290"
     CDS             complement(<1..>3076)
                     /locus_tag="VFF05_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HZW50272.1"
                     /translation="VVNGSWALDEDGKTLRFPYIKADATYAVRIKDTVAAADGKTLGN
                     SVDRDVYTGPMEPAAGFASQGSVLPARDSRGLPVVSVNVKDVDVQFFRVRDGELSNFF
                     AAYQRNGKRSGWDLDPQYGWYGRKGKPLTQIAESVYANRFTLDGKQNERALSYLPIQN
                     IAELSKPGLYFAAMRRAGSLRDQYDTSFFFVSDIGLHTRAYKDTLFVHTASLKTGAPL
                     GDVDLSVVDAKGNTVVAGRTGADGNALLAYALKADEVLTAKSGKDVSILPFNQPALDV
                     SDFAVAGRKQEWFDVFAWSGRDLYRPGETVRLSALLRDYDGNPIKPQPLFATLKQPDG
                     RPYAEAKLEAKDLGYFDWSRTIPVDAPTGKWQVEFRLDPASKEATQSIAFRIEEFLPE
                     RLKLDLTSATPTIAPGEPLKLEVESDYLYGAPAAGNRFTARLTLAPDVHPVEAHADYF
                     FGDPTLDLPKEAKDVVDDTLDEHGKLSADVAILDGKKPTAPVAAIVSGSVYETGGRTV
                     TRTLKRTVWPADALVGVRPLFDPKDGSNANARAGFELIRSDAKGDLLAASKLSVKLVR
                     ENRAYHWTYDNDSGWHFDYNVNYEDAQSQSIDIPAGGTAKIDVPVEWGNYRLDVTDSA
                     TGLTSRFPFFAGWSWNDDNRGKEARPDKIKLALDKPSYRAGDTLRVTVTPPHAGPGVL
                     LIESDHLLYTRDIDAKDGATFEIPVTKDWERHDVYLTALVFRGGSAADKITPARAVGE
                     AFVPMDRSARKVDVAIDAPKTMKPEIDLPVTVKAPALAGKKAFVTVSAVDVGILNITR
                     FARPDPLGWFFGQRRLGVDAYDLYGRVIESFDGGTAAKLRYGGDMALAALPQARRPTA
                     HVQTVDLFSGAVALDAQGNATVALKMPDFNGTVRVTALVFGENAYGANDTETIVRAPL
                     VAEISTPRVLAPGDKAQVTLDLQNFSGTEREFAVKVSADKLLAIAQGERKVKLADQAK
                     TTLNFPLTAQQGYGVGKIEVSATSLSTSSGDIRINRHFDLPVRAAWPAVLRS"
BASE COUNT          501 a          981 c         1009 g          585 t
ORIGIN      
        1 ctgctgcgca acacggcggg ccacgcggcg cgtaccggca ggtcgaagtg ccggttgatc
       61 ctgatgtcgc cgctcgacgt cgaaagcgat gtcgcgctga cctcgatttt gcccacgcca
      121 tagccctgct gcgcggtcag cgggaaattc agcgtggtct tggcctggtc ggcgagcttg
      181 accttgcgtt cgccctgcgc gatcgcgagc aatttgtccg cactcacctt caccgcgaac
      241 tcgcgttcag tgccggagaa gttctgcagg tcgagggtga cttgtgcctt gtcgccgggt
      301 gcgagcacgc gcggcgtgga aatctccgcg accagcggcg cgcgcacgat ggtttcggtg
      361 tcgttcgcgc cgtaggcatt ttcgccgaac accagcgcgg tgacgcgcac cgtgccgttg
      421 aagtccggca tcttcagcgc gacagtggca ttgccctgcg catcgagcgc gaccgcaccg
      481 ctgaacaaat ccacggtctg cacgtgcgcg gtcgggcgcc gcgcctgcgg cagcgccgcc
      541 agtgccatgt caccgccgta gcgcagtttc gccgccgtgc cgccgtcgaa actttcgatc
      601 acgcggccat acaggtcgta ggcatccacg cccaggcgac gctggccgaa gaaccagccc
      661 aacggatcgg gccgtgcgaa acgcgtgatg ttgaggatgc cgacatcgac cgcggacacg
      721 gtcacgaacg cctttttgcc cgcgagcgcc ggtgccttca ccgtcaccgg caggtcgatc
      781 tctggtttca tggtttttgg cgcatcgatg gcaacatcga ccttgcgcgc gctgcgatcc
      841 atgggcacga aggcctcgcc aactgcgcgc gccggcgtga tcttgtccgc cgccgaaccg
      901 ccgcggaaca cgagcgcggt cagatacacg tcatggcgtt cccaatcctt ggtgaccgga
      961 atctcgaacg tcgcaccgtc cttggcgtcg atgtcgcgcg tgtacagcag gtgatcggat
     1021 tcgatcagca gcacgccagg tccggcatgc ggtggcgtga cggtcaccct tagcgtgtcg
     1081 ccggcgcggt agctcggctt gtcgagcgcg agcttgatct tgtccgggcg tgcttccttg
     1141 ccgcggttgt cgtcattcca gctccagccg gcgaagaacg gaaaacgcga ggtcaggccg
     1201 gtcgcggaat cggtcacgtc gaggcggtaa ttgccccact ccaccggcac gtcgatcttg
     1261 gcggtgccgc cggcgggaat atcgatggac tggctttgcg cgtcttcata attgacgttg
     1321 tagtcgaagt gccagccgct gtcgttgtcg taggtccagt gatacgcgcg gttctcgcgc
     1381 acgagtttca cgctgagctt gctcgccgcg agcaaatcgc ctttcgcgtc gctgcgaatc
     1441 agctcgaagc cggcgcgcgc gttggcgttg gagccatcct tgggatcgaa cagcgggcgc
     1501 acgccgacca gcgcgtcggc cggccacacc gtccgcttga gcgtacgcgt gacggtgcgc
     1561 ccgccggttt cgtagacgct gccggaaacg atggcggcga cgggtgcggt gggtttcttg
     1621 ccgtcgagga tcgcgacgtc ggcgctcagc ttgccatgct catcgagcgt gtcatcgacc
     1681 acgtccttgg cttccttggg caagtccagg gtcggatcgc cgaagaaata atcggcgtgc
     1741 gcctccaccg gatgcacgtc gggcgccagg gtcaggcgcg ctgtgaatcg gttgccggcg
     1801 gcgggagcgc catagaggta atcggactcg acttcaagtt tcagcggctc acccggcgcg
     1861 atcgtcggcg tcgcactggt caggtcgagc ttgaggcgct cgggcaggaa ctcctcgatg
     1921 cggaacgcga tactctgcgt cgcttccttg ctcgccgggt cgaggcggaa ttcgacttgc
     1981 cacttgccgg tcggcgcatc gacggggatt gtgcgtgacc aatcgaagta gcccaggtcc
     2041 ttggcttcga gtttggcttc ggcgtatgga cgcccgtcgg gctgtttcag cgtggcgaac
     2101 aacggctgcg gcttgatcgg attgccgtcg tagtcgcgca gcaacgcgga caagcgcacc
     2161 gtctcgccgg gccggtagag gtcgcgcccg gaccaggcga acacgtcgaa ccattcctgc
     2221 ttgcgcccgg cgacggcgaa atcggaaaca tccagtgccg gctggttgaa cggcagaatc
     2281 gacacatcct tgccgctttt cgcggtcaac acctcgtcgg ccttgagcgc ataggcaagc
     2341 agcgcattgc cgtccgcacc ggtcctgccg gcgacgacgg tattgccctt ggcgtcgacg
     2401 acgctcaagt cgacgtcacc gagcggcgca cctgttttca gcgaggccgt gtgcacgaac
     2461 agcgtgtcct tgtaggcacg cgtgtgcagg ccgatgtcgc tgacgaagaa gaaactcgtg
     2521 tcgtattgat cgcgcaagga tccggcgcgg cgcatcgcgg cgaaatacaa ccccggcttg
     2581 gacaactccg cgatattctg gatcggcagg taggacagcg cgcgttcgtt ctgcttgccg
     2641 tcgagcgtga atcggtttgc gtagaccgat tcggcgatct gcgtcagcgg cttgcccttg
     2701 cggccgtacc agccgtattg cggatcgaga tcccatcccg agcgtttgcc gttgcgctgg
     2761 taggcagcga aaaaattcga cagctcgccg tcgcgcacgc ggaagaattg aacgtcgaca
     2821 tccttgacgt tgaccgatac caccggcagc ccgcgcgaat cacgcgccgg caacacgcta
     2881 ccctgcgagg cgaaaccggc cgccggttcc atcggcccgg tgtagacgtc gcggtcgacc
     2941 gaattgccaa gcgtcttgcc gtcggcggcg gcgacggtgt ccttgatgcg caccgcgtag
     3001 gtcgcgtcgg ccttgatgta gggaaagcgc agggtcttgc cgtcttcgtc cagtgcccaa
     3061 ctgccgttga ccacgc
//
LOCUS       DASRVO010000025         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_208395, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000025 DASRVO010000000
VERSION     DASRVO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208395"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFF05_00295"
     CDS             <1..610
                     /locus_tag="VFF05_00295"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HZW50273.1"
                     /translation="AQKNTTSAFASLSLVLDRHSVVESGIQLDYQSGYLSDPYKLVSV
                     GDALDSDTRPDRRGEAAWLVRYRHAVNENAALHLDTRLAINSWGQHSLMLEANWYQSV
                     SGGWQLVSGLRYYSQSRARFYAPFFAANSGPYFSSDYRLGAFGAVAANANLRKRIGRW
                     EFSAGAERYHASTGYAIGGTTSADPAVVSYSRVFAGLDYHFD"
     gene            643..1512
                     /locus_tag="VFF05_00300"
     CDS             643..1512
                     /locus_tag="VFF05_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZW50274.1"
                     /translation="MGCPCALHLEADATSIAQAAHAAVVVECMRLERKYSHYRDDSLV
                     ARIAASAGSDIEIDDETADLLDFAAALHAQSGGLFDISAGALTRLWDAQNGRLPDPSE
                     ITLALAVSGWRRVQWRRPRLRLDVPGMRLDLGGIVKEYAADRCAKICRDAGVTAGVVD
                     LGGDLALIGPHRDGSAWVVGIKAPHVSGHAAARIELPRGGLATSGDYERALIVDGRRY
                     SHIVDPGSGEPVQSFASVSVVAESCLVAGAAATLAMLMGRQRGGAYLRELGLPHLVIR
                     DDGSIDGDIVRIA"
     gene            1556..>2457
                     /locus_tag="VFF05_00305"
     CDS             1556..>2457
                     /locus_tag="VFF05_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZW50275.1"
                     /translation="MSLNPQQRAAVEYTGGPVLVLAGAGSGKTRVIAEKIAHLVARKG
                     MEPGKIAAITFTNKAAREMRERVGKLVKGAAAEELTLATFHALGLKIAQVEHAKLGLK
                     RGFSVFDADDSAAIVKDLAPAGAKPDALFALRSLISNAKGNGLTPEQAVQSARSARER
                     EAADVYARYQKRLTAFNAVDFDDLIRLPLLLLESDDEVRAGWQERIRYLLVDEYQDTN
                     LAQYRLLKLLAGERGAFTCVGDDDQSIYAWRGANPGNLDELARDWPALKVIKLEQNYR
                     CSGRILRAANALIAHNPHVYEKRL"
BASE COUNT          431 a          779 c          816 g          431 t
ORIGIN      
        1 tgcgcagaag aatacgacct cggcattcgc gagcttgagc ctcgtgctgg atcgccactc
       61 cgtggtcgag agcggcatcc agctcgatta ccagagcggc tatctttccg atccgtacaa
      121 actcgtttca gtcggcgatg cgctggactc ggatacgcgc ccggaccggc gcggcgaggc
      181 agcttggctc gtgcgctatc gccatgccgt gaacgaaaac gcagcgttgc atctggatac
      241 gcgcctggcc atcaacagtt ggggccagca ttcgctgatg ctggaggcga actggtacca
      301 gagcgtgagc ggcggctggc aactcgtgtc gggcctgcgc tattacagcc aaagccgggc
      361 gcggttctat gcaccgttct tcgccgcaaa cagtgggccg tatttttcga gtgactaccg
      421 gctgggtgcc tttggcgcag tcgcggcaaa tgcgaatctg cgcaagcgca tcggccgctg
      481 ggaattctcc gccggcgccg agcgctatca tgccagcacg ggttatgcga tcggtggcac
      541 gacatcggcc gatccggcag tggtcagtta ctcgcgcgtg ttcgctggac tggattatca
      601 cttcgattga acggcgctgt atacaaattc gatttctcca gcatgggctg cccctgtgcc
      661 ctgcatttgg aggcggatgc cacctcgatc gcgcaggccg cgcacgcggc agtcgtggtc
      721 gaatgcatgc ggctggagcg caagtactcg cactatcgcg acgacagtct cgtcgcgcgc
      781 atcgcggcga gcgcgggcag cgacatcgaa attgacgacg aaactgccga cctgctcgat
      841 tttgcggcgg cgttgcacgc acaaagcggc ggattgttcg acatctccgc cggcgcgctg
      901 acacggttgt gggatgcgca gaacgggcgt ttgccggacc cgtccgaaat cacgcttgcg
      961 ctcgcggtca gcggctggcg gcgcgtgcaa tggcggcgtc cacgcttgcg tttggacgtg
     1021 cccggcatgc gcctggacct gggcggcatc gtcaaggaat acgcggcgga ccgctgcgcc
     1081 aaaatatgcc gcgatgcggg cgtgactgcc ggcgtggtcg atctcggcgg cgacctggcc
     1141 ctgatcgggc cgcatcgcga tggctcggcg tgggtcgtcg gcatcaaggc gccgcacgta
     1201 tccggccatg cggcggcgcg catcgaactg ccgcgcggcg ggctcgccac cagcggcgat
     1261 tacgaacgcg cgctgatcgt cgacggccgg cgctattcgc acatcgtcga tccgggcagc
     1321 ggcgaaccag tgcaaagttt cgccagcgtc agcgtcgtcg ccgaaagttg cctcgtcgcc
     1381 ggtgcggcag cgaccttggc gatgctgatg ggtaggcagc gcggcggcgc ttacctgcgc
     1441 gaactcggcc ttccgcatct cgtcattcgc gacgacggca gcatcgacgg cgatatcgtc
     1501 cgcatagcct gaacctgctg cggttacaat gcgcgcccgt tttgtccgcg cgcccatgag
     1561 cctgaatccg caacaacgcg ccgccgtcga atacactggc ggtcccgtgc tcgtgcttgc
     1621 cggcgccggt tccggcaaga cgcgcgtgat tgccgagaag atcgcgcacc tggtcgcgcg
     1681 caagggcatg gagcccggca agatcgccgc gatcacgttc acgaacaagg ctgcgcgcga
     1741 aatgcgcgag cgcgtgggca agctggtgaa aggtgcggcg gcggaagaac tcacgctcgc
     1801 gacgttccac gcgctgggcc tgaagatcgc gcaggtcgag cacgcgaaac tcggtctcaa
     1861 gcgcggtttc tccgtgttcg acgcggacga cagcgcggcg atcgtcaagg atctcgcgcc
     1921 agccggggcc aagcccgatg ccttgtttgc gctgcgcagc ctgatctcga acgccaaggg
     1981 aaatggcttg acgccggagc aggcggtgca gtccgcgcgc agcgcgcgcg aacgcgaggc
     2041 ggcggatgtg tatgcgcgct accagaagcg cctgaccgcg ttcaacgcgg tggatttcga
     2101 cgatctcatt cgcctgccgc tgctattgct ggaatccgac gacgaagtgc gcgccgggtg
     2161 gcaggaacgc atccgctatc tgctcgtcga cgagtaccag gacaccaatc tcgcccagta
     2221 ccgcctgctc aagctgcttg cgggcgaacg cggcgcattc acctgcgtgg gcgatgacga
     2281 tcaatcgatc tacgcttggc gcggcgcgaa ccccggcaac ctcgacgagc tggcgcgcga
     2341 ctggccggcg ctcaaagtga tcaagctcga gcagaactac cgttgcagcg gacgcatcct
     2401 gcgcgccgcg aatgcactga ttgcgcataa tccacatgta tacgaaaagc gcctgtg
//
LOCUS       DASRVO010000026         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_26621, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000026 DASRVO010000000
VERSION     DASRVO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26621"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VFF05_00310"
     CDS             <1..1680
                     /locus_tag="VFF05_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW50276.1"
                     /translation="SVAPSKIKSPITPFTPRALSAGGVDRTIRDFVRCAQLAREAGYD
                     GVEIMGSEGYLINEFLAERTNQRSDDWGGSVENRQRFPIEIVRRTRAALGRDFIIIYR
                     LSMLDLVDNAQRWDEIVQLAKAVEAAGATLINTGIGWHEARVPTIVTSVPRAAFTWVT
                     RKMKEHVRIPLITTNRINMPDVAENVLARGDADMVSMARPLLADPAWANKARAGRTTL
                     INPCIACNQACLDHVFENKRASCLMNPRACFETELVDAPMTKQKRFAVVGAGPAGLAC
                     ATTLAQRGHAVTLFEQAHEIGGQFNLAKRIPGKEEFVEPMRYWGNLLAETGVDVRLNT
                     RADTAMLRAGRFDAVILASGVTPRALKIPGADHAKALSYVDVVLHGKPVGKRVAIIGA
                     GGIGFDVAEFLTQRAPSPTTDIARWSHEWGVDQKYASPGGLAKPAPEASEREVWLLQR
                     TPGNLGKRLNKTTGWVHRAALKMKGVKMIGGVNYERIDDTGLHVTINGKPQTLAVDNV
                     VVCAGQEPRRDLHAGLTAAGIAVHLIGGADVAAELDAKRAIAQGTRLACRL"
     gene            1726..2289
                     /locus_tag="VFF05_00315"
     CDS             1726..2289
                     /locus_tag="VFF05_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50277.1"
                     /translation="MDAFSYLSVLLSIIVGLAITQILTGFRGIMLWHSRVRMFWPALL
                     WAGTLLLINVQSWWAMFSLREVTTWTFAGFAVVLAQAIAQYLLAAIVLPDFSADHTTD
                     LREHYWSHTRWFFGLFVLVLLFSLAKDPVVYGHAPERENLAFHIGFIALSVIALSTRR
                     DGFHKFLALLGAGVFCAYVIALFTQLR"
     gene            complement(2291..>2846)
                     /locus_tag="VFF05_00320"
     CDS             complement(2291..>2846)
                     /locus_tag="VFF05_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014502267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZW50278.1"
                     /translation="STFAARVIPGTGSDMYSAIAGSIGALRGPKHGGANEAAFEIQKR
                     YDTPDAAEADIRKRVENKEVVIGFGHPVYTTGDPRNKVIKEVARELSAAQHNMTMFDI
                     AARLEATMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVISRTSGWSAHVIEQRID
                     GKIIRPSANYTGPENLKFVPLDKR"
BASE COUNT          479 a          929 c          925 g          513 t
ORIGIN      
        1 tcggtcgcgc catcgaaaat caagtcgccg atcacgccat ttacgccgcg cgcgctttct
       61 gccggcggtg tagaccgcac gatccgcgat ttcgtgcgtt gcgcgcagct cgcgcgcgag
      121 gccggctacg acggcgtcga aatcatgggc agcgagggct acctgatcaa cgagttcctc
      181 gccgagcgca cgaaccagcg cagcgatgac tggggcggca gcgtggagaa tcgccagcgc
      241 ttccccatcg aaatcgtgcg ccgcacgcgc gcggcgctcg ggcgcgattt catcatcatc
      301 tatcgactgt cgatgctgga cctcgtcgac aacgcgcaac gctgggacga aatcgtgcaa
      361 ctggccaagg ccgtcgaggc tgccggcgcg acgctgatca acaccggcat cggctggcac
      421 gaggcgcgcg tgccgaccat cgtgaccagc gtgccacgcg cggccttcac ctgggtcacg
      481 cgcaagatga aggaacacgt gcgcattccg ctgatcacga cgaatcgcat caacatgccg
      541 gacgtcgccg aaaacgtgct ggcgcgcggc gacgcggaca tggtgtcgat ggcgcgtccg
      601 ctgctggcgg atcccgcctg ggcgaacaag gcgcgcgccg gccgcacaac cctgatcaat
      661 ccctgcatcg cctgcaatca ggcctgcctg gaccatgtgt tcgagaacaa gcgcgcctcg
      721 tgtctgatga acccgcgcgc ctgtttcgaa accgagctcg tcgacgcgcc gatgacgaag
      781 caaaaacgct tcgccgtggt cggcgccggc cctgccggac tcgcttgcgc gacgacgttg
      841 gcgcagcgcg gacatgccgt gaccttgttc gagcaggcgc acgaaatcgg cggccagttc
      901 aacctggcca agcgcattcc gggcaaggag gaattcgtcg agccgatgcg ttattggggc
      961 aacctgctcg ccgaaaccgg ggtcgacgtg cggctcaaca cccgcgccga tacggccatg
     1021 ctgcgggctg gcagattcga tgccgtgatc ctcgcctccg gcgtcacccc gcgcgccctg
     1081 aagattcccg gcgcggatca cgccaaggcg ctgagctacg tcgatgtcgt cctgcacggc
     1141 aaaccggtcg gcaagcgtgt cgccatcatc ggcgccggcg gcatcggttt cgacgtggcg
     1201 gaattcctga ctcaacgggc gccctcgccg accaccgaca tcgcgcgctg gtcgcatgaa
     1261 tggggcgtcg atcaaaagta tgcgagcccc ggcgggctcg ccaagcccgc gccggaggcc
     1321 agcgaacgcg aagtctggct gctgcagcgc acgccgggga acctcggcaa gcgcctgaac
     1381 aagaccaccg gctgggtgca ccgtgcggcg ctgaagatga agggcgtgaa gatgattggt
     1441 ggcgtgaatt acgagcgcat cgacgacacc ggcctgcacg tcacgatcaa cggcaagccg
     1501 caaacgcttg ccgtggacaa cgtcgtcgtc tgcgccggcc aggaaccgcg tcgcgacctg
     1561 cacgccggac tcaccgctgc cggcatcgcc gtgcacctga tcggcggtgc ggacgtcgcc
     1621 gccgagctcg acgccaagcg cgcgatcgcg caaggcacgc gcctcgcgtg ccgtttatga
     1681 ttcgttgcac gcggctggcg gcaacgctct gagggaaccg ggacgatgga cgccttcagt
     1741 tatctgtcgg tgctgctgtc gatcatcgtc ggcttggcga tcacgcagat cctgaccggc
     1801 ttccgcggca tcatgctatg gcactcgcgc gtgcgcatgt tctggccggc gctgctgtgg
     1861 gccgggaccc tgctgctgat caatgtgcaa agctggtggg cgatgttcag cctgcgcgag
     1921 gtaacgacct ggacattcgc cgggttcgcc gtggtgctgg cgcaagccat cgcgcaatac
     1981 ctgctcgcgg ccatcgtgct gccggatttt tccgccgatc acacaaccga cttgcgcgag
     2041 cactactggt cgcacacgcg ctggttcttc ggcctgttcg tgctggtttt gcttttcagc
     2101 ctggcaaagg acccggtggt gtatgggcac gcgcccgagc gcgagaacct ggcgtttcac
     2161 atcggcttca tcgcgctgtc ggtcatcgcg ttgtccacgc gccgcgacgg ttttcacaag
     2221 tttctcgcac tgctcggcgc gggcgtgttt tgcgcctacg tgattgcgct gttcacgcaa
     2281 ttgcgctagt tcagcgcttg tcgagcggca cgaacttgag attttccggc ccggtgtagt
     2341 tcgcgctagg gcggatgatc ttgccgtcga tgcgctgctc gatcacatgc gcgctccaac
     2401 ccgaggtgcg cgaaatcacg aacagcggcg tgaacatcgc agtcggcacg cccatcatgt
     2461 ggtagctcac ggcgctgaac cagtcgaggt tcgggaacat cttcttgatg tcccacatcg
     2521 tggcttccag acgcgcggcg atgtcgaaca tcgtcatgtt gtgctgcgcg gcggacagct
     2581 cgcgcgcgac ttccttgatg accttgttgc gcggatcgcc ggtggtgtag accggatggc
     2641 cgaagccgat cacgacttcc ttgttttcca cgcgcttgcg gatgtcggcc tcggccgcgt
     2701 ccggcgtgtc gtagcgcttc tggatttcga acgcggcctc gttcgcgccg ccgtgcttgg
     2761 gtccgcgcag cgcgccgatg gaaccggcga tggccgaata catgtcgcta ccggtaccgg
     2821 gaatgacgcg cgcggcgaat gtcgag
//
LOCUS       DASRVO010000027         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_208500, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000027 DASRVO010000000
VERSION     DASRVO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208500"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1097
                     /locus_tag="VFF05_00325"
     CDS             30..1097
                     /locus_tag="VFF05_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZW50279.1"
                     /translation="MPHTAFSATHPPQSLCLLRTSALGDVTHVVPLLRTLQGAWPQTR
                     LTWIVGKLEHKLVGDIPGVEFVVFDKAAGWCGFAALRRQLAGRRFDVLLHLQVALRAN
                     LVSALARADLRIGYDKARSKDLHGLFVNARIAQRSGEHVLDAMASFLEPLGLKQTTVD
                     WNIPIPDSAREFAARHLPGDVATLLVSPCSSHRLRNWLPDRYATIMDHAAACGWRVVL
                     CGGPNSFEREFGNAIVEKCINKPIDLIGKDTLKQFLALCQRASIVLTPDSGPMHMANA
                     VGTKVLGLHAPSNPERSGPYSDRRWCVNRYDAAARKFRGKAAAELPWGTKLEYPGAME
                     LIAVDDVIERFEAFATESGRG"
     gene            complement(1094..>1606)
                     /locus_tag="VFF05_00330"
     CDS             complement(1094..>1606)
                     /locus_tag="VFF05_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZW50280.1"
                     /translation="LVLNHNEAAGRVDTVADPRWRYLRNDEQARWRRVLDAVTPADTT
                     LGRLREDISCRVVRILHAAGVTVLAGTDSPMPRGYPGYALHDELELLAGCGLSSAQVL
                     RAATLGATELLGIEASSGTVEVGKRADLVLLDANPLADVRNLRRIRAVVLGGRLLTGS
                     DLARLIDEQP"
BASE COUNT          267 a          552 c          519 g          268 t
ORIGIN      
        1 gcctcatcat cgccagttta gcagcccgca tgccgcacac cgcgttttcc gccacccatc
       61 ccccgcaatc gctgtgcctg ttgcgtacct cggcgctggg cgacgtgacg cacgtggtgc
      121 cgctgctacg cacgctgcaa ggcgcgtggc cgcagacccg actgacctgg atcgtcggca
      181 agctcgaaca caagctggtc ggcgacattc ccggcgtgga attcgtcgtc ttcgacaagg
      241 ccgccggttg gtgcggattt gccgcgttac gccggcaact ggccggacgc cgcttcgacg
      301 tactgctgca tctgcaggtc gcgctgcgcg cgaacctggt cagcgcgctg gcgcgtgcgg
      361 acctgcgcat cggctacgac aaggcccgct cgaaagatct gcacggcctg ttcgtgaatg
      421 cgcgcatcgc gcagcgcagc ggcgagcacg tgctggatgc gatggcgagt tttctcgaac
      481 cgctgggcct gaagcaaacg accgtggatt ggaacatccc gattccggat tccgcacgtg
      541 aattcgccgc caggcacctg cccggcgatg tcgcgacctt gctggtgagt ccctgctcca
      601 gccatcgcct gcgcaactgg ctgccggatc gctacgccac catcatggat cacgccgctg
      661 cgtgcggatg gcgcgtcgtg ctgtgcggtg gaccgaattc gttcgaacgc gagttcggca
      721 acgcgattgt ggaaaagtgt ataaataaac ccatcgacct gatcggcaag gacacgctca
      781 agcaattcct cgccctgtgc cagcgtgcca gcatcgtgct gacgccagat tccgggccga
      841 tgcacatggc caacgcggtc ggcaccaagg tgctcggcct gcatgcgccg agcaatccgg
      901 agcgctcggg accctactcc gaccggcgct ggtgcgtgaa ccgctacgac gcggcggcgc
      961 gcaaattccg cggcaaggcg gcggcggaac tgccctgggg aacgaagctg gaatatccgg
     1021 gcgcgatgga attgatcgcg gtcgacgatg tgatcgaacg cttcgaggcg ttcgcgaccg
     1081 aatcgggtcg cggctagggc tgctcgtcga tcagtcgcgc gaggtcgctt cccgtcagca
     1141 gacggccgcc gagcaccacc gcgcgtatcc ggcgcaggtt gcgtacgtcc gccagggggt
     1201 tggcatccag cagcacgagg tcggcgcgct tgccgacctc gaccgttccc gaggacgcct
     1261 cgatcccgag caactcggtg gcgcccagtg tcgccgcacg caggacttgc gccgagctga
     1321 gaccgcagcc cgccagcaat tcgagttcgt cgtgcaaggc gtagcccgga tagccccgcg
     1381 gcatcggcga gtccgtcccg gccagcacgg tcacgcccgc cgcgtgcaga atgcgcacga
     1441 cgcggcaact gatgtcctcg cgcaacctgc ccagcgtcgt atccgccggc gtcaccgcat
     1501 ccagtacgcg gcgccagcgc gcctgttcgt cgttgcgcag ataacgccag cgcgggtcgg
     1561 ccacggtgtc cacacgtccc gccgcttcgt tgtggttcag caccag
//
LOCUS       DASRVO010000028         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_312452, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000028 DASRVO010000000
VERSION     DASRVO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312452"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFF05_00335"
     CDS             <1..477
                     /locus_tag="VFF05_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HZW50281.1"
                     /translation="GTGLGSKAASWWAVVPSSPQRAVYSPLAFLGGIVAAVLAVFLIV
                     RLVYHGHVRRSAPWNCGFPPQTARMQDTAEGFGQPIRRIFGPVFRIRRQTPSPFDTAP
                     RYFLDLGDRWWDWVYDPIARAVKASAARIGVLQQGSIALYLLYSFATLVALLAFVL"
     gene            474..1424
                     /locus_tag="VFF05_00340"
     CDS             474..1424
                     /locus_tag="VFF05_00340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HZW50282.1"
                     /translation="MSATNILLGLFQALLSILAAPLLLGWVDQCRAWLQNRRGAGVLQ
                     PYRVLRKLVHKDAVIAHGASALFRASPYVQFGAMCLAAAIIPVVSITAPLATAADTIA
                     LVGLFALARVFIALAGMDIGTAFGSLGARREMLIGFLSEPALLMVFFTASLICGSTLL
                     PTIVDTLAHREFAIYPSLAFAGVAFTMVLLAENARIPIDNPATHLELTMIHEAMVLEY
                     SARHLALIEWASALKLMNFMAIGLALFVPWGLTAATTGPRGLAVGIVATALKLGVAGA
                     ALALIETASAKLRIFRAPEFLATAFLFAVLGLLVHQLLGS"
     gene            1426..2091
                     /locus_tag="VFF05_00345"
     CDS             1426..2091
                     /locus_tag="VFF05_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HZW50283.1"
                     /translation="MPTPTWASQLINLGAALLLLSSFAMLSQRRILSLVNLFALQGAV
                     LSASTAIVAHTSGATHLYASALLSLILKVIVLPWILHRLIARLNVRWDTETQLNIPAT
                     MLVGIVVVVFAFALAQPIAQLSATVTRSTLGIALAVVLLSFLMMITRRKAVSQVIGFL
                     AMENGLFFAATSATYGMPMVVELGVALDVLVGTLILGVFFFQIREQFDSLDLHHIEQL
                     RED"
     gene            2091..>2600
                     /locus_tag="VFF05_00350"
     CDS             2091..>2600
                     /locus_tag="VFF05_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HZW50284.1"
                     /translation="MALLLLLAIPLAGSLALAVFGARRWAAELNAAFSIATFAAACAL
                     TARVIAEGPMLVAHEQFFVDPFNVFLIALTAFVGMTTSLFSRPYMRVEEAHGRVNRRR
                     LRLYHSMYQLFCFTMLAALSTNNLGILWVAMEAATLSTVLLVSLYRTAASIEAAWKYF
                     ILCGVGIAQA"
BASE COUNT          352 a          908 c          818 g          522 t
ORIGIN      
        1 ggtaccgggc tcgggtccaa ggcggcttcg tggtgggcgg tggtgccgtc gtccccgcaa
       61 cgtgcggtgt acagcccgct cgcgttcctc ggcggcatcg tcgccgcggt gctcgccgtg
      121 ttcctgatcg tgcgccttgt ctaccacggc cacgtgcggc gcagcgcacc atggaactgc
      181 ggatttcctc cgcaaaccgc gcgcatgcag gacacggcgg aaggctttgg ccagccgatc
      241 cggcgcatct tcggccccgt gttccgcatc cgccggcaga cgccgtcgcc gttcgacacg
      301 gcgccgcgct atttcctgga tctcggcgac cgctggtggg actgggttta cgacccgatc
      361 gcgcgcgccg tgaaagccag cgccgcgcgc atcggcgtat tgcagcaagg cagcatcgcg
      421 ttgtacctgc tttacagctt cgccacgctc gtggcactgc tggcgttcgt gctgtgagcg
      481 ccacgaatat cctgctcggc ctgttccagg cgctgctgtc gatcctcgcc gcgccgttgc
      541 tgctgggctg ggtcgatcag tgccgcgcct ggttgcagaa ccggcgcggc gccggcgtgc
      601 tgcagcctta tcgcgtgctg cgcaagctcg tgcacaagga cgccgtgatc gcgcacggcg
      661 catcggcatt gttccgcgct tctccgtacg tgcaattcgg cgcgatgtgc ctggccgccg
      721 cgatcatccc ggtcgtcagc atcaccgcgc cgctcgccac ggccgcggac acgatcgcgc
      781 tggtcggcct gttcgcgctc gcccgcgtct tcatcgcgct ggccggcatg gacatcggca
      841 ccgccttcgg cagcctcggc gcgcgccgcg aaatgctcat cggctttctc tccgaacccg
      901 cgctgctgat ggtgttcttc accgcctcgc tgatctgcgg tagcaccctg ctgccgacaa
      961 ttgtcgacac gctcgcgcac cgcgagtttg cgatttatcc gagcctggcc tttgccggcg
     1021 tcgcgttcac catggtactg ctcgccgaga acgcacgcat tccgatcgac aacccggcca
     1081 cgcacctgga actgaccatg atccacgagg cgatggtgct ggaatattcc gcccgccacc
     1141 tggcgctgat cgaatgggcc tccgcgctga agttgatgaa cttcatggcg atcgggctcg
     1201 cgctgttcgt gccgtggggg ctcactgccg cgacaaccgg cccgcgcggc ctcgcggtcg
     1261 gcatcgtcgc caccgcgctc aagctcggcg tcgctggtgc cgcgctcgcc ctgatcgaaa
     1321 ccgcgagcgc gaagctgcgc atcttccgcg cgccggaatt cctcgccacc gcgttcctgt
     1381 tcgcggtact tggtctgctc gtccaccagt tgctcggaag ctgacatgcc gacacccacc
     1441 tgggcatcgc agttgatcaa cctcggcgcg gcgctgttgc tgctgtcgtc gttcgcgatg
     1501 ctgtcgcagc gacgcattct ttcgctggtg aacctgttcg cgctgcaggg cgccgtgctg
     1561 tccgcatcca ccgcgatcgt cgcgcacacc tcgggtgcca cgcacctgta cgcatcggcg
     1621 ctgctgtcgc tgatcctcaa ggtcatcgtg ctgccgtgga tcctgcatcg cctgatcgcg
     1681 cgcctgaacg tgcgctggga taccgaaacg caactgaaca tcccggcgac gatgctggtc
     1741 ggcatcgtcg ttgtcgtttt cgcattcgcg ctcgcccagc cgatcgcgca attgtcggcc
     1801 acggtgacgc gcagcacgct cggcatcgcg ctcgcggtgg tcctgctgtc gttcctgatg
     1861 atgattacgc ggcgcaaggc agtgtcgcag gtgatcggct tcctggccat ggaaaatggc
     1921 ctgttcttcg ccgcgaccag cgccacctat ggcatgccga tggtggtgga attgggcgtc
     1981 gcgctcgacg tgctcgtcgg cacgctgatc ctgggcgtgt tcttcttcca gatccgcgaa
     2041 caattcgaca gcctcgacct gcaccacatc gaacagctcc gggaggactg atggcgctgc
     2101 tcctgctgct cgcaatcccg ctcgccggca gcctcgcgct cgccgtgttc ggcgcacgcc
     2161 gctgggcggc ggaactcaac gccgcgttca gcatcgccac gttcgcggcc gcgtgcgcgc
     2221 tcaccgcgcg ggtgatcgcc gagggtccaa tgttggtggc gcacgaacag tttttcgtcg
     2281 acccgttcaa tgtgttcctg atcgcgctga ccgcgttcgt cggcatgacc acgtcgctgt
     2341 tctcgcgtcc gtacatgcgc gtggaggagg cccacggccg cgtcaaccgg cgccgcctgc
     2401 gcctgtacca cagcatgtac cagctgttct gcttcacgat gctggcggcg ctgtcgacga
     2461 ataatctcgg catcctctgg gtggcaatgg aagcggccac gctgtcgacc gtgctgctcg
     2521 tgtcgctgta ccgtaccgcg gcaagcatcg aagcggcgtg gaaatacttc atcctgtgcg
     2581 gcgtcggcat cgcgcaggcg
//
LOCUS       DASRVO010000029         1340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_208592, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000029 DASRVO010000000
VERSION     DASRVO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208592"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /gene="hemW"
                     /locus_tag="VFF05_00355"
     CDS             <1..1116
                     /gene="hemW"
                     /locus_tag="VFF05_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZW50285.1"
                     /translation="WCLRKCPYCDFNSHEVPGGELPQQRYLDALVADIESALPIVWGR
                     EIHSIFIGGGTPSLFSPAGIDRLLADIRARLKLAADCEITLEANPGTFERDRFRAFRS
                     AGVTRLSVGVQSFNDEHLKALGRVHDRAQAIAAAQEAAAAFDTFNLDLMYALPGQTMA
                     QLEEDLTQALALMPPHLSVYHLTIEPNTYFAKFPPRVPDEDTAYAMLDRITEMTSGAG
                     LERYEVSAYAKPGHRCWHNVNYWQFGDYLGVGAGAHSKLTFAHRIVRQVRLREPRLYM
                     DGALAGRPLAQDDEVARADLPFEFMLNALRLRDGFALAQFTERTGLALTAIARPLEQA
                     QRKGLVERDLARVRPTTRGFDFLSDLQALFLPAAKRP"
BASE COUNT          209 a          471 c          447 g          213 t
ORIGIN      
        1 tggtgcctgc gcaagtgccc ctactgcgac ttcaattcgc acgaggtgcc aggcggcgag
       61 ctgccgcagc agcgctatct cgacgcgctg gtggccgaca tcgaaagcgc gctgccgatc
      121 gtgtggggcc gcgagatcca cagcatcttc atcggcgggg gcacgcccag cctgttctcg
      181 ccggccggca tcgaccgtct gctggccgac atccgcgccc gcctgaagct ggccgccgat
      241 tgcgagatca cgctggaggc caatcccggc acgttcgaac gcgaccggtt ccgcgccttc
      301 cgcagcgccg gggtgaccag gctgtcggtc ggtgtccaga gcttcaacga cgaacacctg
      361 aaggcgctgg gccgcgtgca tgaccgggcg caggcaattg ccgccgccca ggaggcggcc
      421 gcggccttcg acacgttcaa cctggatctg atgtatgcgc tgcccggcca gacgatggcg
      481 cagctcgaag aggacctcac ccaggcactc gcgctgatgc cgccgcacct gtcggtgtat
      541 cacctgacga tcgagccgaa cacgtatttc gcgaagttcc cgccgcgcgt gcccgacgag
      601 gacacggcct acgccatgct ggatcggatc accgagatga cgtccggggc cggtctggag
      661 cgctacgaag tttccgccta cgcgaaaccc ggccatcgct gctggcacaa cgtgaactac
      721 tggcaattcg gcgactacct gggcgtcggc gccggtgcgc acagcaagct cacgtttgcg
      781 caccgcatcg tgcgccaggt gcgcctgcgc gaaccccgcc tgtacatgga cggcgcgctc
      841 gcgggacggc cgctggcgca ggatgacgag gtcgcgcgcg ccgacctgcc gttcgaattc
      901 atgctgaacg cgctgcgttt gcgcgacggc ttcgcgctcg cccagttcac cgagcgcacg
      961 ggactggcac tcacggcgat cgcgcggccg ctcgagcaag cgcaacgcaa aggcctcgtc
     1021 gagcgcgatc tcgcgcgcgt gcgtccgacc acgcgcgggt tcgactttct gagcgacctg
     1081 caggccctgt tcctgccggc cgcgaagcgt ccttgacctg aagcaatgga tcgcgcggcc
     1141 cggatgcatt cttcgcatgg cgggcggcgg atcgggcatt tgcggtttgc ctcggccgca
     1201 cctagattca cggtgctgta cgctcgggca cgcgcccggc gcgcgtggcg tatgaaggga
     1261 tcacccccat gaatgcatcg acgcaggctg tgcacgaccg gctcgccgcc gtcctggccc
     1321 tggccgccgc gcggctggag
//
LOCUS       DASRVO010000030         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_312531, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000030 DASRVO010000000
VERSION     DASRVO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312531"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1106)
                     /gene="pheT"
                     /locus_tag="VFF05_00360"
     CDS             complement(<1..>1106)
                     /gene="pheT"
                     /locus_tag="VFF05_00360"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZW50286.1"
                     /translation="MAGLEVEAVDALGGGLDGVVVGEIVECAPHANADKLRVCQVSVA
                     KGAPLQIVCGAPNARVGLKAPLATIGAKLPNGVEIKQAALRGVESNGMLCSAKELALD
                     ADASGLLELSADAPAGKPLAEYLALPDARIELKLTPNRPDCLGLRGLAHDVAALFDTA
                     VKEPSTAPVAAVSDSKREVKLDAGADCPRYVGRVIEGVNAAAKSPLWLAERLRRSGIR
                     PISAIVDVTQYVMLELGQPMHAYDMARLEGPIHVRHARAGEIVKLLDGNEHALAPEFL
                     VIADAKNTLGLAGIMGGFDSRVVDTTRDIFLEAAHFAPAAIMGRARKLGLHTDASHRF
                     ERGVDPELPRLAMERATRLIVDICGGKPGPITEA"
BASE COUNT          182 a          378 c          357 g          189 t
ORIGIN      
        1 ggcctcggta atcggacccg gcttgccgcc gcagatatcg acgatcaggc gcgtggcgcg
       61 ctccatcgcc aggcgcggca actccggatc gacgccgcgt tcgaaacgat gcgaagcgtc
      121 ggtgtgcagg ccgagtttgc gcgcgcggcc catgatcgcg gccggcgcaa aatgcgcggc
      181 ttcgaggaag atgtcgcgcg tggtatcgac gacgcgcgaa tcgaatccgc ccatgatgcc
      241 ggcaaggccg agcgtgttct tggcatcggc gatgacgagg aattccggcg ccagcgcgtg
      301 ctcgttgccg tcgagcaact tcactatttc gcccgcgcgc gcatgacgta cgtggatcgg
      361 accttcgagc ctggccatgt cgtaggcatg catgggctga ccaagttcca gcatcacgta
      421 ttgcgtgacg tcgacgatcg cgctgatcgg ccggatgccg ctgcggcgca ggcgctcggc
      481 cagccacagc ggcgacttcg cggcggcatt cacgccttcg atgacgcgac ccacgtagcg
      541 agggcaatcc gcgccggcat ccagcttcac ttcgcgcttc gagtcggata ccgcggcgac
      601 cggcgccgtt gatggttctt tcaccgccgt gtcgaacagc gcggcaacgt cgtgcgcgag
      661 tccgcgcaat cccaggcagt cgggccggtt cggcgtgagt ttcaattcga tgcgcgcatc
      721 gggcagcgcc aggtattccg cgagcggttt gccggctggc gcgtcggccg acagttcgag
      781 caatcccgaa gcgtcggcat cgagcgcaag ctccttggcc gaacacagca tgccgttcga
      841 ctcgacgcca cgcagtgccg cctgcttgat ttcgacaccg tttggcagtt tcgcgccgat
      901 cgtggccagc ggcgccttga gcccgacgcg tgcattcggc gcgccacaga cgatctgcaa
      961 cggcgcaccc ttggcgacgg atacctggca cacgcgcagc ttgtccgcgt tcgcatgcgg
     1021 cgcgcattcg acgatctcgc cgacgacaac gccgtccaat ccgccgccga gcgcatcaac
     1081 cgcctcgact tccaacccgg ccatcg
//
LOCUS       DASRVO010000031         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_26788, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000031 DASRVO010000000
VERSION     DASRVO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26788"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VFF05_00365"
     CDS             <1..1506
                     /locus_tag="VFF05_00365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW50287.1"
                     /translation="HLPFLEIVADNGTLISSAQWPARFGYMEPWVREFSDWNAQPAFL
                     AREELPDGPVLALVAVRTVRVADKTLYLIGGQPVDREFLSSIVLPEGTRLMLYTGVGT
                     PFNPAALAVSPGELKDAGKLAPIIAQVEQKPAETWSTIAWSSGAADRETIHALPLQGR
                     DGELLAVLLLGSSRREVAELNSHIRSIALLVGGGAILLGILFSTWLASRVTRPVEELA
                     EASRRVAAGDWEAHVENESRDELGQLADSFNTMTRQLVEQRGRLVQAERVAAWRELAR
                     RLAHELKNPLFPLQITVENLLRARQAPPADFDEVFRESTTTLLAEINNLKAIVGRFSD
                     FAKMPRPELRTVAVNDLVREVARVFQAQLAAPDRPKVALDLQLGDPPDVLADRDLLHR
                     ALSNLMLNALDAMPQGGSLTIRTQAREGGVRIEISDTGSGLTQEECDRLFTPYYTTKQ
                     HGTGLGLAIVQSVVSDHGGRISVRSAPGGGTTFSIDLLREPATDAARGTHA"
BASE COUNT          269 a          551 c          526 g          260 t
ORIGIN      
        1 cacctgccgt tccttgaaat cgttgccgat aacggcacgc tcatttcttc cgcgcagtgg
       61 ccggcgcgtt ttggctacat ggaaccttgg gtgcgcgagt tctccgactg gaacgcgcag
      121 cctgcattcc tggcccgcga agaactgccc gacggcccgg tgctggcgct ggtcgcggtg
      181 cgcaccgtgc gcgtcgcgga caaaaccctg tacctcatcg gcggccagcc cgtggaccgc
      241 gagtttctct cttccatcgt gctgcccgag ggaacgcgcc tgatgctcta taccggagtc
      301 ggaacaccgt tcaacccggc ggcgctggcg gtttcccccg gcgaattgaa ggatgcgggc
      361 aagctggcgc cgatcatcgc gcaagtcgag cagaagcccg ccgaaacctg gagcacgatc
      421 gcgtggtcct ccggcgcggc ggaccgagag accatccacg cgctgccgct gcaaggccgc
      481 gacggcgaac tgctggcggt actgctgctg ggcagttcgc ggcgcgaagt cgccgaactc
      541 aactcgcaca tccgctccat cgcgctgctg gtgggcggcg gcgccatcct gctgggaatt
      601 ctgttcagca cctggctggc ctcgcgcgtc acccgtccgg tggaggagtt ggcggaggcc
      661 tcgcggcgcg tcgcggcggg cgattgggag gcgcacgtcg agaatgaatc gcgcgacgaa
      721 ctcggccagc tcgccgattc gttcaacacc atgacccggc aactcgtcga gcagcgcggc
      781 cgcctggtgc aggccgagcg cgtggccgcc tggcgcgaac tggcgcggcg cctggcgcac
      841 gagctcaaga atccgctctt cccgctgcag atcacggtgg aaaacctgtt gcgcgcacgc
      901 caagccccgc ctgccgactt cgacgaagtc ttccgggaaa gcacaaccac gttgctggcg
      961 gaaatcaaca atctgaaggc gatcgtcgga cgcttcagcg actttgccaa gatgccgcgg
     1021 cccgagctgc gcacggttgc ggtcaacgac ctggtgcgcg aagtggcgcg cgtcttccag
     1081 gcgcaactgg ccgcgccgga ccgcccgaaa gtcgcgctgg acctgcaact cggcgacccg
     1141 ccggacgtcc tcgccgaccg cgatctgctg caccgcgcct tatccaacct aatgctgaac
     1201 gcactcgacg ccatgccgca gggcggatcg cttactattc gtacccaggc gcgcgagggt
     1261 ggggtgcgca tcgagatttc cgacaccgga tcgggactga cgcaggagga atgcgaccgc
     1321 ttgttcactc cttattacac caccaagcag cacggcaccg ggctgggact ggccatcgtg
     1381 cagtcggtgg tcagcgacca cggcggtcgc atcagcgtgc gcagcgcgcc cggcggcggc
     1441 acgaccttta gcattgacct gctgcgtgag ccggccaccg atgccgcgcg ggggacgcat
     1501 gcctgagacg aaagcgcgcc tgctcatcat tgacgacgag cccaacacgc tggcatcgct
     1561 atcgcgcgcc tttcgcctcg ctgggcacga ggccgtggtg tgcgac
//
LOCUS       DASRVO010000032         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_53133, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000032 DASRVO010000000
VERSION     DASRVO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53133"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFF05_00370"
     CDS             complement(<1..471)
                     /locus_tag="VFF05_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZW50288.1"
                     /translation="MPDNPFRRTKPEPIPPVAASAGEKTLPLHINGKDFFHNGDPDMP
                     LLWYLRDVLRLTGAKYGCGTGICGACTVLIDGKAEAACMFPVKALAHRDIVTIEGLAG
                     KDALHPLQEAWIAEDAIGCGYCQTGQIMAAAALLKRKPHPDDADIDAIPNLCRCG"
     gene            619..>1282
                     /locus_tag="VFF05_00375"
     CDS             619..>1282
                     /locus_tag="VFF05_00375"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973914.1"
                     /note="catalyzes the 4-phospho-L-aspartate from
                     L-aspartate and the formation of lysine from
                     meso-2,6-diaminoheptanedioate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HZW50289.1"
                     /translation="MNHPAATPVPDPHAPWIVLKFGGTSVSTRARWDKIAAIARAWRE
                     HGKNVLIVVSALSGITDKLKALGEAQADAAKRAAICDEIRARHEAMFTELGLVDRAPL
                     QYWLERLDALAANVRADAGGLPWQAEVLALGEQLSSTLGVAYLNRLDLPAHWLDAREH
                     LLAQPMPNQNAWGRYLCASVPTAPDAAFAQGLAARGSLFVTQGFMARNPQGETVILGR
                     GGS"
BASE COUNT          205 a          421 c          434 g          222 t
ORIGIN      
        1 gccgcagcgg cacaggttcg gaatcgcgtc gatatccgca tcgtcgggat gcggcttgcg
       61 cttgagcaac gccgctgccg ccatgatctg cccggtctgg caataaccgc agccgatcgc
      121 atcctcggcg atccaggctt cctgcagcgg gtgcagcgcg tctttgccgg ccaggccctc
      181 gatggtgacg atgtcgcggt gtgcgagcgc cttcaccgga aacatgcagg cggcttcggc
      241 cttgccgtcg atgagcaccg tgcaggcgcc gcagatgccc gtgccgcaac cgtacttcgc
      301 gccggtcagg cgcagcacgt cgcgcaggta ccacagcagt ggcatgtccg ggtcgccgtt
      361 gtggaagaaa tccttgccgt tgatgtgcag cggcagggtc ttttcgccgg cgctggcggc
      421 gaccggcgga atgggctctg gcttggtgcg gcggaaggga ttgtcaggca tgacgaaacc
      481 gtatgcgtgg acggctattt tgcaccatca agatgatgtt tgccgacgaa atgtcaggag
      541 tcggcccgca tcgcgccttg tttttttgcg ccgcacaagg acaatgacgg ttccgctttc
      601 acgccgaaga ccgtcccgat gaaccatccc gccgccacgc ccgtgcccga cccgcacgcg
      661 ccgtggatcg tgctcaagtt cggcggcacc agcgtgtcaa cgcgtgcgcg ctgggacaag
      721 atcgccgcca tcgcacgcgc ctggcgcgag catggcaaga acgtgctgat cgtggtgtcg
      781 gcgctgtccg gcatcaccga caagctcaag gcgctcggcg aggcgcaggc cgacgccgcc
      841 aagcgtgccg ccatctgcga cgaaatccgc gctcgccacg aagcgatgtt caccgaattg
      901 ggattggtcg accgcgcccc gctgcagtac tggctcgaac gtctcgacgc gctggccgcg
      961 aacgtgcgtg ccgatgccgg cggactgccg tggcaggccg aagtgctggc gctcggcgag
     1021 cagttgtcga gcacgcttgg cgtggcttat ctgaaccgac tggatctgcc ggcgcactgg
     1081 ctcgacgcgc gcgagcacct gcttgcgcaa cccatgccga accagaacgc gtggggacgc
     1141 tatctctgcg cctcggtgcc gaccgcgccg gacgcggcat tcgcgcaagg cctcgccgcg
     1201 cgcggctcgc tgttcgtcac gcaagggttc atggcgcgca atccgcaggg cgaaacggtg
     1261 attctgggtc gcggcggttc gg
//
LOCUS       DASRVO010000033         1043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_286811, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000033 DASRVO010000000
VERSION     DASRVO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286811"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VFF05_00380"
     CDS             complement(<1..785)
                     /locus_tag="VFF05_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HZW50290.1"
                     /translation="MRHDLIATRERLFAGQASATAEMAESIRIAQSPACERAFLHTAF
                     EEAQRLAAQPGATAGALAGLAVSVKDLFDLAGQPTPAGSRVLADAPAATADCAAIARL
                     KAAGGIVIGRTNMSEFAFSGIGTNPHFGTPANPADPVTPRIPGGSSCGAAVSVASGAA
                     FIGLGSDTGGSIRIPAALCGIVGFKNTARLTPAQGALPLSTTLDTVCAMTRSVRDAIV
                     AHEILSGRSVARSQLPLSGYRLALARTTMLDDMDRTVATAFERA"
     gene            complement(841..>1043)
                     /locus_tag="VFF05_00385"
     CDS             complement(841..>1043)
                     /locus_tag="VFF05_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50291.1"
                     /translation="LGIPEARLVPEMDTGFQHFTHRDCHGFSSKVGSKIQPEIAAAFE
                     GGDNTLWGLDSRLVLPGESAQQ"
BASE COUNT          171 a          338 c          372 g          162 t
ORIGIN      
        1 gcacgttcga aagcggtggc caccgtacga tccatgtcgt ccagcatggt cgtgcgcgcg
       61 agcgccagcc gatagccgga cagcggcaac tggctgcgcg cgacgctgcg gccggacagg
      121 atctcgtgcg cgacgatcgc gtcgcgcacc gaacgcgtca tcgcacagac cgtgtcgagc
      181 gtcgtggaca agggcagcgc accttgcgcc ggcgtcaggc gcgcggtgtt cttgaatccg
      241 acgatcccgc acagggccgc cgggatgcgg atcgagccac ccgtgtcaga gcccaggccg
      301 atgaacgccg cgccgctggc gaccgagacg gcggcgccgc aggacgagcc ccccgggatg
      361 cgcggggtca ccggatcggc cgggttggcc ggcgtgccga agtgcggatt cgtgccgatc
      421 ccggaaaacg cgaactccga catgttcgtg cggccgatga cgatgccgcc ggcagccttc
      481 agccgggcga tggcggcgca atccgcggtc gcggccgggg catcggcgag cacgcgcgag
      541 ccggccggtg tcggctggcc ggccagatcg aacaggtcct tgaccgagac ggccaggccc
      601 gccagtgctc cagccgtcgc gcccggctgc gccgccaggc gctgcgcttc ttcgaacgcc
      661 gtgtgcagga atgcgcgttc gcaggcgggt gactgggcaa tgcggatcga ctcggccatc
      721 tcggctgtgg cgctggcctg cccggcgaag agtcgttcgc gggtggcgat gaggtcgtgg
      781 cgcatggggg tccgtcgggg caggagtgct ataatttttc ggttttgccg ggtgctcgcg
      841 ctattgctgc gccgattcgc cgggcaaaac caatcgcgaa tcgagccccc acaaggtgtt
      901 gtcgccgcct tcaaacgcag cagcaatttc gggctggatt ttagacccaa ccttcgagga
      961 aaacccatgg cagtcacgat gcgtgaaatg ctggaagccg gtgtccattt cgggcaccag
     1021 acgcgcttct ggaatcccaa gat
//
LOCUS       DASRVO010000034         1315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_27009, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000034 DASRVO010000000
VERSION     DASRVO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27009"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VFF05_00390"
     CDS             <1..761
                     /locus_tag="VFF05_00390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161388.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZW50292.1"
                     /translation="EVTGTVSFGKDTKGKQRLVITDVDGVANEFLIPKEKHVLAHDGQ
                     VVNQGEMIVDGPADPHDILRLLGIEALAQYITDEVQDVYRLQGVKINDKHIEVIVRQM
                     LRRVVVSDPGDSKFLQEEQVERAEVLDENDKLIAEGKRPAMFIDILLGITKASLSTDS
                     FISAASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGMAYHQARKAKDQMD
                     EAERRAIAESEAAELASATEAATAPEASEGAASE"
     gene            830..>1315
                     /locus_tag="VFF05_00395"
     CDS             830..>1315
                     /locus_tag="VFF05_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50293.1"
                     /translation="MPSPVVLGAIAALLLFWTVGAYNRLMRLRAEANTAFAGLDAELA
                     KQVALVRECLPPAEATQPATLEDAGSSFWAGLHGAAAQFAASLAAARTRPLEPDGIEA
                     LSAAQDVLAMAWERAERDDAHDLAGARLPESITARRAQLAMQTHVAIDQFDRAVGRYN
                     EA"
BASE COUNT          243 a          437 c          438 g          197 t
ORIGIN      
        1 ccgaagtgac tggcacggtt tcgttcggca aggacaccaa gggcaagcag cgactcgtca
       61 tcaccgatgt ggatggcgtc gccaacgaat tcctgattcc caaggagaaa catgtcctgg
      121 ctcacgatgg ccaggtggtg aatcagggtg aaatgatcgt ggacggcccg gccgatccgc
      181 acgacattct gcgtttgctg ggtatcgaag cgctggcgca atacattacc gacgaggtgc
      241 aggacgtgta ccgtttgcag ggcgtgaaga tcaacgacaa gcacatcgaa gtcatcgtgc
      301 gccagatgct gcgtcgcgtc gtggtctccg atccgggcga ttccaagttc ctccaggaag
      361 agcaggtcga acgcgccgaa gtgctggacg agaacgacaa gctcatcgcc gaaggcaagc
      421 gtccggcgat gttcatcgac atcctgctcg gcatcaccaa ggccagcctg tccaccgact
      481 cgttcatctc cgcggcttct ttccaggaaa ccacgcgcgt gttgaccgaa gcggcgatca
      541 tgggcaagcg cgacgagctg cgcggcctga aggaaaacgt catcgtcggc cggctgatcc
      601 ccgccggcac cggcatggcg taccaccagg cgcgcaaggc gaaggaccag atggacgagg
      661 ccgagcgccg cgccatcgcc gagtccgaag ccgcggaact ggccagcgcc accgaagcgg
      721 cgaccgcacc cgaggccagc gaaggcgcgg cgagcgaata gcccggcgct aaactagcgc
      781 cccagcccgt cccgggttgg ggcgcttttt ttttggattg ccgcgggcca tgccatctcc
      841 cgtcgttctc ggtgcgatcg ccgcgctgct gctgttctgg accgtgggcg cgtacaaccg
      901 cctgatgcgt ttgcgcgcgg aggcgaacac ggcgtttgcc ggcctcgacg ccgaactcgc
      961 gaagcaggtc gccctcgtgc gcgaatgcct gccgccggcc gaagcgaccc aaccggccac
     1021 cctggaggat gccggcagct cgttctgggc cggcttgcat ggcgcggcgg cgcagttcgc
     1081 ggcatcgttg gccgcggcgc gcacgagacc gctcgaaccc gacggcatcg aggcgctgag
     1141 cgccgcgcag gacgtgctcg ccatggcatg ggagcgcgcc gagcgcgacg acgcccacga
     1201 cctcgcgggg gcgcgcctgc ccgaatcgat caccgcgcgc cgggcccagc tcgcgatgca
     1261 gacgcatgtg gccatcgacc agttcgaccg cgcggtcggc cgctacaacg aagcg
//
LOCUS       DASRVO010000035         2249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_182879, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000035 DASRVO010000000
VERSION     DASRVO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182879"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFF05_00400"
     CDS             complement(<1..423)
                     /locus_tag="VFF05_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50294.1"
                     /translation="MALDIRTLIMLAAVVALLNGASLRYVLREYPADLSPSIRLWTLG
                     VLAQATAWILFGLRDQIPDWLSIVGANSLLSLAFAEQVHAVRLFGGLPVRRAAVYAPA
                     VAVLVDEIVFTYAMPSMRLRLLTATPWFCLQMIQGGLSL"
     gene            516..1295
                     /locus_tag="VFF05_00405"
     CDS             516..1295
                     /locus_tag="VFF05_00405"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZW50295.1"
                     /translation="MDIAPRTPEPSTAAIARDRDRVFWASVGAAGLVAGIWLAWLGAW
                     LLGWDMDDLGIRPRDAHGLVGILTAPFVHASFAHLMSNTLPLGLLAALALYAYPRATR
                     LALPFIWIVSGIGVWLWARPSIHVGISGIAHGLMFFLFFMGLLRRDRLAIAIALAVFF
                     LYGGMVMTVLPRDPQVSFEYHLFGALAGLIAAFVWHQRDPQPPRKRYSWEIEEEEAER
                     SATDPEFDLPRPENVPALWDGPRASARGGDNVVPLQPHREE"
     gene            1391..1654
                     /locus_tag="VFF05_00410"
     CDS             1391..1654
                     /locus_tag="VFF05_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50296.1"
                     /translation="MPVRRNQQSDGARRSTKWFSRSGQRHLYALFMPPRQIFTKSLFH
                     GVQIGGQSKEAGPVDALYLLLLVLLIAGTAGFLRACARLEPKK"
     gene            1737..1829
                     /gene="kdpF"
                     /locus_tag="VFF05_00415"
     CDS             1737..1829
                     /gene="kdpF"
                     /locus_tag="VFF05_00415"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HZW50297.1"
                     /translation="MNLVYWISALIAVALSIYLLAALLKPEWFE"
     gene            1826..>2249
                     /locus_tag="VFF05_00420"
     CDS             1826..>2249
                     /locus_tag="VFF05_00420"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZW50298.1"
                     /translation="MTTNDIIQLGLYLVVLLLLVRPLGSYMAAVFSDAPNRVTRIGAP
                     LERALYRVSGIAPDEEMTWTRYAWAMLIFNIAGLLVVYALLRLQHWLPLNPQKMENVP
                     PDLAMNTAISFATNTNWQAYSGESTLSYLSQMLGLSVQN"
BASE COUNT          369 a          757 c          713 g          410 t
ORIGIN      
        1 gagcgacagg ccgccctgga tcatctgcag gcagaaccat ggcgttgccg tgagcaggcg
       61 cagacgcatg cttggcatgg cgtaggtgaa aacgatctcg tcgaccagca ccgcaacggc
      121 gggagcgtag accgccgcgc gcctgaccgg caggccgccg aacagtcgaa ccgcatggac
      181 ctgctcggca aacgccaggc tcagcagcga gttcgcgccc acgatcgaca gccagtccgg
      241 gatctggtcg cgcaggccga acaggatcca tgcggtggcc tgggccagga cgccgagcgt
      301 ccacaggcgg atgctgggag acaggtcggc cggatactcg cgcagcacat agcgcaggct
      361 tgcgccgttg agcagggcca cgacggcagc cagcatgatc agggtgcgaa tatccagcgc
      421 cacgtcatcc cccttctggc gaatctagtc tgcgccggtc gctggtgcgg cgcaaatgcc
      481 ttgtcgacgc cgcttgcagg ccccattgtc gaccgatgga catcgcgccg cgcactccag
      541 agcccagtac tgccgcgatc gcgcgcgacc gcgaccgtgt gttctgggcg agcgtcggcg
      601 cagcgggact cgtcgccggc atctggctgg cctggctcgg ggcgtggctg ctcggctggg
      661 acatggacga cctcggcatc cgcccgcgcg atgcgcacgg cctggtcggc atcctcaccg
      721 cgccgttcgt gcatgcctcg ttcgcgcacc tgatgtcgaa caccttgccg ctgggcctgc
      781 tcgccgccct cgcgctctac gcctatccgc gcgcgacgcg tctcgcattg ccgttcatct
      841 ggatcgtctc cggcatcggc gtgtggctgt gggcgcggcc gtcgatccac gtcggcatca
      901 gcggcatcgc gcatggcctc atgttcttcc tgttcttcat gggcctgctg cgccgcgacc
      961 gcctcgccat tgccatcgcc ctggccgtgt tcttcctgta cggcggtatg gtcatgaccg
     1021 tgttgccgcg cgatccgcag gtgtcgttcg aataccatct gttcggcgcg ctcgccggtc
     1081 tgatcgccgc gttcgtctgg caccagcgcg atccgcaacc gccgcgcaag cgctatagct
     1141 gggaaatcga ggaagaagag gcggagcgct ccgcaacgga tccggaattc gacctgccgc
     1201 ggccggaaaa cgtgccggcg ctctgggacg gcccgcgcgc gtcggcacgc ggcggcgaca
     1261 atgtggtgcc gctgcagccg caccgcgaag aatgaaactc ccgggcgcgc gggcaagccg
     1321 ccgccggatc tgacacgctg acgcggatcg cgacaccgcg gccgtccttg tcccgcattc
     1381 gatcccgtcg atgccggttc gacgcaacca gcaaagtgat ggcgcccgac gatccaccaa
     1441 gtggttttca aggtcgggcc agcggcatct ttatgcgctt tttatgccgc cgcgccagat
     1501 atttacgaaa tcgctattcc acggggtcca aattggcggc cagtcaaaag aggccggtcc
     1561 cgtggatgcc ctgtatctgc tgctactcgt cctgctgatc gccggcacgg ccggttttct
     1621 tcgcgcgtgc gcacgccttg agccgaagaa atgagcgcgc tctgtggcgc gatcggaacg
     1681 acgtcgcccc ttgccgtcaa ggcgcaattc gcaccgggct tctaccagga gtggcaatga
     1741 acctcgtcta ctggatttcc gcgctgattg cggttgcgct cagcatctat ctgctcgccg
     1801 cgttgctcaa accggagtgg ttcgaatgac gacgaacgac atcatccagc tcggcctgta
     1861 tctcgtcgtc ctgctgctgc tcgtccggcc gctcggcagc tacatggctg ccgtgttctc
     1921 cgatgcgcca aaccgcgtca cgcgcatcgg tgcaccgctc gagcgtgcgc tctaccgcgt
     1981 cagcggtatc gctccggacg aagagatgac gtggacgcgc tacgcatggg ccatgttgat
     2041 tttcaacatc gccggcctgc tcgtcgtcta tgcgctgctg cggctgcaac actggctgcc
     2101 gctcaatccg caaaagatgg agaacgtgcc gccggacctg gcgatgaaca cggccatcag
     2161 tttcgccacg aacacgaact ggcaggcgta cagcggcgaa tcgactctga gttatctgtc
     2221 gcagatgctc ggcctgagcg tgcagaatt
//
LOCUS       DASRVO010000036         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_261013, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000036 DASRVO010000000
VERSION     DASRVO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261013"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFF05_00425"
     CDS             <1..767
                     /locus_tag="VFF05_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZW50299.1"
                     /translation="RDELGDLARSFSRLLEESAAYTDYLRTLASKLSHELHTPLAIVK
                     SSLDNLDHQTLPVDAQPYLTRARDGAERLGAIVRAMSQASRMERAIAAAEPEDFDLAA
                     VVRGCVDSYRPLVAPRRLDADLPFDPAPMHGAPELIAQALDKLIDNARGFAPETGWIR
                     IALTATHDGARIAVANSGPPLPPAMQDRLFDSLVSVRDAGARGDAPHLGFGLFVVRLI
                     AQAHRGGASARNLDDGNGVEFVLELRGMARRPLAGS"
     gene            834..>2067
                     /locus_tag="VFF05_00430"
     CDS             834..>2067
                     /locus_tag="VFF05_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008575582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZW50300.1"
                     /translation="MLSAEQFATFTAQGHNRIPVVREVLSDLDTPLSVYLKLADGPFA
                     YLFESVEGGARWGRYSIIGLPARRVIRVYGRRLTVTEEGETVEDRVVADPLVEIAALK
                     AQYRVPQIEGLPKFAGGLVGYFGYECASYIEPKIRADAKPDQLGMPDICLMLAEELAV
                     FDNLKGKLYLIVNADPGEPRAYARAQRRLDSLVHRLRSTSAAYPDAVDPKAIDESHFV
                     SSFPKDDYLAAVAKAKEHVFAGDVFQVQISQRLSVPFRARPLDVYRALRALNPSPYMY
                     YIDLGGEQIIGSSPEILVRAQGGQVVLRPIAGTRRRGHTPEEDTAMEAELLADPKERA
                     EHVMLIDLGRNDVGRVAQTGSVELTANMLVERYSHVMHIVSEVVGKLKPDLNFMDVFR
                     ATFPAGTLTGAPKVRAMEI"
BASE COUNT          350 a          719 c          655 g          343 t
ORIGIN      
        1 cgcgcgacga actcggcgac ctcgcacgca gtttctcgcg cctgctcgag gaatcggccg
       61 cctacaccga ctacctgcgt acgctcgcga gcaagctttc gcacgaactg cacacgccac
      121 tcgcgatcgt caaatcctcg ctcgacaacc tcgatcacca gaccctgcca gtcgatgcgc
      181 agccgtacct gacgcgcgca cgcgacggcg ccgagcgcct gggcgcgatc gtgcgcgcta
      241 tgagccaggc cagccgcatg gaacgcgcga tcgccgcggc cgaacccgaa gacttcgatt
      301 tggcagcggt cgtgcgcggc tgcgtggaca gctatcgtcc actcgtcgcg ccgcgccgtc
      361 tcgatgccga tctgcccttt gatcccgcgc cgatgcatgg cgccccggaa ctgatcgcgc
      421 aggcgctgga caagctcatc gacaacgcgc gcggcttcgc gccggaaacg ggctggatcc
      481 gcatcgccct caccgccacc cacgacggcg cacgcatcgc cgtggccaac agcggtccgc
      541 cgctgccgcc ggcaatgcag gatcgcctgt tcgattcgct ggtgagcgtg cgcgatgccg
      601 gcgcgcgcgg cgacgcgccg cacctgggct ttggcctgtt cgtggtgcgc ctgatcgcgc
      661 aggcgcatcg cggaggcgcc agtgcgcgca acctcgacga cggtaacggc gtggaattcg
      721 tgctggagtt gcgtgggatg gcgcgcaggc cgctggcggg ttcctgacca tcggggctgt
      781 cgccgcggcg tgcggcttgc tatcgtcccc tgcccgactc gcccggactc cgcatgctct
      841 ccgccgaaca attcgccacc ttcaccgcgc aaggccacaa ccgcattccc gtggtacgcg
      901 aggtattgag cgatctggac acgccgctgt cggtgtattt gaaactcgcc gacggcccgt
      961 tcgcgtatct gttcgaatcg gtcgaaggcg gtgcgcgctg gggacgatat tccatcatcg
     1021 gcttgcccgc acggcgtgtg attcgcgtat acggcaggcg cctgaccgtg accgaggaag
     1081 gcgaaaccgt ggaggatcgc gtcgtcgccg atccgctcgt cgagatcgcc gcactcaagg
     1141 cgcaataccg tgtgccgcaa atcgaaggcc tgccgaaatt cgccggcggc ctggtcggct
     1201 atttcggcta cgaatgcgcg agctacatcg agccgaagat ccgcgccgac gccaaacccg
     1261 accaactcgg catgcccgac atctgcctga tgctggctga ggaactggcg gtcttcgaca
     1321 acttgaaggg taagctctac ctcatcgtca acgccgatcc cggcgagcca cgcgcctacg
     1381 cgcgcgcgca acggcggctc gattcactgg tgcatcgcct gcgttcgacc agcgccgcgt
     1441 atccggacgc ggtcgatccc aaggccatcg acgaatcgca tttcgtgtcg agttttccga
     1501 aagacgatta cctcgccgcc gtggcgaagg cgaaggagca cgtcttcgcc ggcgacgttt
     1561 tccaggtgca gatcagccag cgcctgtcgg tgccgttccg ggcgcgaccg ttggacgtct
     1621 accgtgcgct gcgcgcgctc aatccctcgc cgtacatgta ctacatcgac ctcggcggcg
     1681 agcagatcat cggttcgtcg ccggaaatcc tcgtgcgcgc gcagggcggt caggtcgtgc
     1741 tgcggccgat cgccggcacg cgccggcgcg ggcacacgcc ggaggaagac acggcaatgg
     1801 aggccgaact gctcgccgat cccaaggaac gcgccgagca cgtgatgttg atcgacctcg
     1861 gccgcaacga cgtcggccgc gtggcgcaaa ccggcagcgt ggaactcacc gcgaacatgc
     1921 tcgtggagcg ctactcgcac gtcatgcaca tcgtctcgga agtcgtcggc aagctgaaac
     1981 cggatttgaa tttcatggac gtgttccgcg ccaccttccc cgccggcacg ttgaccggcg
     2041 cgcccaaggt gcgtgccatg gaaatca
//
LOCUS       DASRVO010000037         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_130767, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000037 DASRVO010000000
VERSION     DASRVO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130767"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFF05_00435"
     CDS             <1..259
                     /locus_tag="VFF05_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZW50301.1"
                     /translation="AQIRARVSQRKIRCISAMDAQAIAQWPGVRSAARDGERVEIVSD
                     AAESVVRRLLDQDAQLQELEIQRAGLAEAFVEITKVAADAA"
     gene            363..1142
                     /locus_tag="VFF05_00440"
     CDS             363..1142
                     /locus_tag="VFF05_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW50302.1"
                     /translation="MNAIAQTTVMPPIAYRPLPAYLMEAKYEFLRLLRTPSFAVPTLS
                     FPPLFFLLFGLLLNHGNAGAAQYLFATYSVFGVMAPSLFGFGVAVAIEREKGWLALKR
                     VAPMPPGAYLLAKLVMAMLCAAIIYLVLAAMAYMLGGVRLPPVQWFALGSVAVSGALP
                     MCALGLIIGSRANASAAPAFVNLIYLPMSFLSGLWMPLSMMPHFLAQIAPLWPAWHLG
                     QLALMATGQATMGSIAGHLLWLAVFTAICFAIARRWLARVA"
     gene            1241..2413
                     /locus_tag="VFF05_00445"
     CDS             1241..2413
                     /locus_tag="VFF05_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZW50303.1"
                     /translation="MPSLLSSLLHPAPDSLAARSRQRGKSPWIDAIHLLWTAWIFVTP
                     AFDHDSWNLRWITITALSYPVFLALYARCVMASRRTSLGYAIGMILLCQVLMPWYPSG
                     LSYFVFGCVMLRLCGQPVRRYMGALVVAGSLLLLEGWLLHYPWQVTGTIATMTFIIGL
                     IVNVERTSEEKDAELRLSHDEVRRLAATAERERIGRDLHDLLGHTLSLITLKSELANK
                     LFDRDAAGAKREMADVERVARDALAQVRRAVTGIRAAGFAAELASAKLLLESNGVHLD
                     YELADAGMPAEVETALAMTVREAVTNIQRHARATRASIALECADGRLILRIADDGRGG
                     DISPGNGLTGMRERLAAIGAELHVESQRGHGTTLTASLPMPQQEARIATAIELRSA"
     gene            2410..>2602
                     /locus_tag="VFF05_00450"
     CDS             2410..>2602
                     /locus_tag="VFF05_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW50304.1"
                     /translation="MIRVLLAEDQAMVRGALSALLKLESDLDVIGCAADGEEAWLLAQ
                     REKPDVVVTDIEMPRLTGLE"
BASE COUNT          415 a          836 c          843 g          508 t
ORIGIN      
        1 ggcgcaaatc cgcgcacgcg tgtcgcagcg gaaaatccgc tgcatcagcg cgatggatgc
       61 gcaagcgatc gcgcagtggc ccggcgtgcg cagcgctgcg cgcgatggcg agcgcgtgga
      121 aatcgtctcc gatgcggccg aatccgtggt gcgccgcctg ctcgatcagg atgcgcaact
      181 gcaggagctg gaaatccagc gagcgggact tgctgaagca tttgttgaga ttacgaaggt
      241 cgcagccgac gcggcttaag cgatgcctcg aatggcatcg cctgcatcgg cgagggtgag
      301 gcgggatgcc gaacggaaga gcgccacgga tggttccaat caattcaata cgaaggtgcg
      361 taatgaacgc gatcgctcaa accactgtca tgccgcccat cgcctatcgc ccgcttccag
      421 cctacctcat ggaagccaaa tacgaattcc tgcgtttgct gcgcacgccg tcgttcgcgg
      481 tgccgacact gtcgttcccg ccgctgttct tcctgttgtt cggcctgctg ctcaatcacg
      541 gcaacgcggg cgccgcgcaa tacctgttcg ccacctacag cgtgttcggc gtgatggcac
      601 ccagcctgtt cgggtttggc gtggcggtgg cgatcgaacg cgaaaagggc tggctggcgc
      661 tcaagcgtgt cgcgccgatg ccgccgggtg cgtacttgtt ggccaagctc gtcatggcga
      721 tgctgtgtgc ggccatcatc tatctcgttc tcgccgcgat ggcgtacatg ctgggcggtg
      781 tgcgtctgcc gcctgtgcaa tggttcgcgc tgggcagcgt tgcggtgtct ggcgcactgc
      841 cgatgtgtgc gctcggactc atcatcggca gccgcgccaa cgcctcggcc gcaccggctt
      901 tcgtgaacct gatctacctg ccgatgtcct tcctgtccgg gctgtggatg ccgctgtcca
      961 tgatgccgca tttcctcgcg cagatcgcgc cgctgtggcc ggcctggcat ctgggccagc
     1021 tcgctctgat ggcaaccggg caggcgacga tgggtagcat cgccgggcat ctgctttggc
     1081 tggccgtgtt taccgcgatc tgcttcgcaa tcgcgcggcg ctggttggcg cgagtggcat
     1141 gagtcagggc agcgtcatgc gcgtgatcta ttgtcgtttt gccttcaccc tcgcggcggt
     1201 ggtattactg ctcgccagcc tgcaccccat ccactgaccc atgccgagtt tattgagcag
     1261 tttgttgcac ccggcgcccg attcgctggc ggcacgcagc cggcagcgcg gcaagtcgcc
     1321 gtggatcgat gctatccacc tgctctggac cgcatggatt tttgtcacac cggccttcga
     1381 ccacgattcg tggaatctgc gctggatcac gatcacggct ctgtcctatc cggtctttct
     1441 ggcgctgtac gcccgatgcg tgatggcttc gcgccgcacc tcgctcggct acgcgatcgg
     1501 catgatcctg ctgtgtcagg tgctgatgcc gtggtatccc agtggcttga gctacttcgt
     1561 attcggttgc gtgatgctgc gtctctgcgg ccaacccgtt cgccgctaca tgggcgcgct
     1621 ggtagtcgct ggctcgctgc tgctcctcga aggctggctg ctgcactatc cgtggcaggt
     1681 cacaggcacg atcgcgacga tgaccttcat catcggcctg atcgtcaatg tcgagcgcac
     1741 gagcgaggag aaggatgccg agttgcgcct gtcgcacgac gaggtgcgcc gcctggccgc
     1801 cactgccgag cgcgaacgca tcggccgcga cctgcacgac ctgctcggcc acacgctgtc
     1861 gctgatcacg ctcaagtcgg agcttgcgaa caagttgttc gaccgcgatg ccgcaggcgc
     1921 caaacgcgaa atggccgatg tggaacgcgt cgcacgcgac gcgctggcgc aggtccggcg
     1981 tgcggtgacc ggcatccgtg ccgcgggctt cgctgcggaa ctggcatcgg cgaagctgct
     2041 gctcgaatcc aacggcgtgc atctggacta cgaactcgcc gacgctggca tgccggccga
     2101 ggtcgaaacc gcactcgcga tgaccgtgcg cgaggcagtg accaacatcc agcgccatgc
     2161 gcgcgccacg cgcgcgagca tcgcgcttga atgtgccgac ggtcgattga tcctgcgcat
     2221 tgccgacgat ggccgtggcg gcgacatctc gcccggcaac ggcctgaccg gcatgcgcga
     2281 acgccttgcc gcgatcggcg cggaactgca cgtggagtcg cagcgcggcc acggcaccac
     2341 gctgaccgca agcctgccga tgcctcagca ggaagccagg atcgccactg cgatcgaatt
     2401 gcgcagcgca tgattcgcgt ccttctcgcc gaagaccagg ccatggtgcg cggcgcgctg
     2461 tcggcgttgc tcaagctcga atcggatctg gacgtgatcg gctgtgccgc agacggcgag
     2521 gaagcctggt tgctcgcgca acgtgagaag cccgatgtcg tcgtcaccga cattgaaatg
     2581 cctaggctca ccgggctgga gc
//
LOCUS       DASRVO010000038         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_992, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000038 DASRVO010000000
VERSION     DASRVO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1923
                     /gene="dsbD"
                     /locus_tag="VFF05_00455"
     CDS             <1..1923
                     /gene="dsbD"
                     /locus_tag="VFF05_00455"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HZW50305.1"
                     /translation="ATQAVIELRYQGCHQVTPKICFPPQKKILAVPMPSASASAAPAS
                     LRAALGAATGEASSSLLGGNQPLPAEQAFKFEAIATGPGEILARFTMPKGYYLYRDKT
                     NFSVAADSGVTLGAPTFPPGKDHSDANFGQSTVYFDQVEIPVPLARTNMAAHTLALTA
                     NFQGCKEGSVCYPMISSTLDVNLPAGAGADISASSTAPGAATANANANADSRFQNALN
                     GSLWLVFGAFFLAGLGLAFTPCVFPMIPILSGNIAGAGENISTRRAIVLSLVYILANA
                     VIFTIAGVIAGLAGNNLQAAFQTPWVLWCFAGLFVLLALSMFGFYELQLPGSWQSRIA
                     NVSNKQSGGSLWGVAIMGALSALIVGPCVTPPLAVAVLYIAQKHDAVLGGFALFALAL
                     GMGAPLVVFGASAGKLLPRAGAWMDAVKAVFGVTFLGLAIWMLSRVVPASWIMLMTGL
                     LAVACAVYLGALERLADGVSGWKKLWKALGVVLLIGGAAELIGAAAGSNDLLQPLRGA
                     GGGAVTAPSEQGLAFRRIKSLADVEQALAAAKSTGQPAMLDFYADWCVSCKEMEKFTF
                     TDARVKQALAGFVLLQADVTANDDTDKALMKHFEIVGPPDTVFYDADGKQRHDLQLVG
                     AEKPGDFVQRVGGVRR"
BASE COUNT          340 a          687 c          680 g          392 t
ORIGIN      
        1 gcgacccagg ctgtcatcga attgcgctac caaggctgcc atcaggtcac gccgaaaatc
       61 tgtttcccgc cgcagaaaaa aatcctggcc gtgccgatgc cttcggcttc ggcatcggcg
      121 gcgccggcat cgttgcgcgc ggcgctgggc gcggccactg gcgaagcgtc gtcttcgctg
      181 ctcggcggca atcagccgct gcccgcggaa caggctttca aattcgaggc aattgcgacc
      241 ggtcccggcg agattctcgc gcgcttcacg atgccgaagg gttattacct gtatcgcgac
      301 aagacgaact tttccgtagc agcagacagc ggcgtcacgc tcggcgcgcc aacgtttccg
      361 ccgggcaagg atcacagcga cgcgaatttc ggccagtcca cggtgtattt cgaccaggtc
      421 gagattccgg ttccgcttgc gcgcacgaac atggccgcgc acacgctcgc actaaccgcg
      481 aatttccagg gctgcaagga aggcagcgtg tgctatccga tgatatccag cacgctcgac
      541 gtgaaccttc cggccggtgc gggcgcggac atttccgctt ccagcacagc gccgggcgcg
      601 gccaccgcca acgccaacgc caacgccgac agccgattcc agaacgcgct gaacggttcg
      661 ctgtggctgg tgttcggcgc gttcttcctg gccggcctcg gcctcgcatt cacgccctgc
      721 gtattcccga tgatcccgat cctgtcgggc aacatcgccg gcgcgggcga gaacatttcc
      781 acgcgccgtg caatcgtctt gtcgctggtc tacatcctcg ccaacgcggt gatcttcacc
      841 atcgccggcg tgatcgcggg cctggccggg aacaatttgc aggccgcgtt ccagacgccg
      901 tgggtgctgt ggtgcttcgc cggcctgttc gtgctgctcg cactgtcgat gtttggcttc
      961 tacgaattgc agttgccggg ttcgtggcaa tccaggatcg cgaacgtcag caacaagcaa
     1021 tccggcggct cgctgtgggg cgtcgcgatc atgggcgcgt tgtccgcgct gatcgtgggg
     1081 ccatgcgtca caccgccgct ggccgtcgcc gtgctctaca tcgcgcagaa acacgatgcc
     1141 gtgctcggcg gctttgccct tttcgcgctc gcgctcggca tgggcgcgcc gctggtggtg
     1201 ttcggcgcca gcgccggcaa gctgctgccg cgcgccggtg catggatgga cgcggtcaag
     1261 gccgtgttcg gcgtgacctt cctcggcctt gcgatctgga tgctctcgcg cgtggtaccg
     1321 gcgtcgtgga tcatgctcat gacaggcctg cttgcggtgg cctgtgccgt atacctcggc
     1381 gcactcgaac gcctggcgga tggcgtttcc ggctggaaaa aattgtggaa ggcgctgggc
     1441 gtcgtgctgc tgatcggcgg cgcggcggaa ctcatcggcg cggcggccgg ctccaacgat
     1501 ctgctgcaac cgctgcgcgg tgccggcggc ggcgcggtca cggcgccatc cgaacaggga
     1561 ctggcgtttc gccggatcaa atcgcttgcc gacgtcgaac aggcgctggc ggcggcgaaa
     1621 tcgaccggcc aacccgccat gctcgatttc tatgccgact ggtgtgtgtc ctgcaaggaa
     1681 atggagaaat tcacattcac cgatgcgcgc gtcaaacagg cgctggcggg gttcgtgttg
     1741 ctgcaggccg acgtcaccgc gaacgacgac accgacaagg cgctgatgaa gcatttcgag
     1801 atcgtcggcc cgccggacac cgtcttctac gacgcggatg gcaagcaacg ccacgacctg
     1861 caactggtcg gcgcggagaa gcccggcgat ttcgtgcagc gcgtcggtgg cgtacggcgg
     1921 taaaatgccg gcatgtacaa atcaaacctc ttcatcatcc tcattgcgat cgccggcgcc
     1981 ctgctcggcc tctatgtcgg cagctatttc agccggccgc aggtggccgg gctggcaacg
     2041 ggcacgggcg tgctcggccc gggccagcag ctggccgatc tgcagctaac cggcaccga
//
LOCUS       DASRVO010000039         1110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_234772, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000039 DASRVO010000000
VERSION     DASRVO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234772"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /gene="hypD"
                     /locus_tag="VFF05_00460"
     CDS             complement(<1..766)
                     /gene="hypD"
                     /locus_tag="VFF05_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HZW50306.1"
                     /translation="MKYVDEFRDGELAKNIAANIARESNAQRSYRLMEFCGGHTHAIS
                     RYGIADLLPANVRMIHGPGCPVCVLPIGRIDQAIRLAREHGVILCTYADTMRVPASDY
                     LSLMRAKASGADVRMIYSTQDALNIARANPQREVVFLAIGFETTTPPTAVAVSQAAAE
                     GIGNFSILCDHVLTPAAMHAILADGVELDGFVGPAHVSTVIGSKPYEPFAGDYGKPVV
                     IAGFEPLDVMQAILMLVRQMNEERAAVENEFTRAVTR"
     gene            complement(778..1032)
                     /locus_tag="VFF05_00465"
     CDS             complement(778..1032)
                     /locus_tag="VFF05_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZW50307.1"
                     /translation="MCLAIPARIEELTGEGNAIVDLGGVRKEISLALVEDANVGDYVI
                     VHVGYALQKLDRAEAERTLELFAEMGALDELREALDGSRI"
BASE COUNT          186 a          367 c          346 g          211 t
ORIGIN      
        1 cacgcgttac ggcgcgcgtg aattcgttct cgaccgcggc gcgctcttcg ttcatctggc
       61 gcactagcat caggatcgcc tgcatcacgt cgagcggctc gaaacccgcg atcaccaccg
      121 gcttgccgta gtcgccggca aacggttcgt acggcttgct gccgatcacg gtggatacgt
      181 gcgctggacc gacgaaaccg tcgagctcga cgccgtcggc gaggatcgcg tgcatcgcgg
      241 ccggggtcag cacgtggtcg cacaggatgc tgaaattgcc gattccctcg gccgccgcct
      301 ggctgaccgc caccgcggtc ggcggagtgg tggtttcgaa gccgatggcg aggaacacca
      361 cttcgcgttg cggattggcg cgcgcgatgt tcagcgcatc ctgcgtcgag tagatcatgc
      421 gcacgtccgc gccgctggcc ttggcgcgca tcagcgacaa ataatccgaa gcgggcacgc
      481 gcatggtgtc ggcataggtg cacaggatca cgccatgttc gcgcgccagc cggatcgcct
      541 ggtcgatacg gccgatcggc agcacgcaca ccggacagcc gggaccgtgg atcatgcgca
      601 cgttggccgg cagcaggtcg gcgatgccgt agcgcgagat ggcatgggta tggccgccgc
      661 agaactccat caggcggtag ctgcgctgcg cattggattc gcgcgcgatg ttggcggcaa
      721 tgttcttcgc cagttcgccg tcgcggaatt cgtcgacgta tttcatcccc tggctactca
      781 tatccggctg ccatccagtg cttcgcgcaa ctcgtccagc gcgcccatct cggcgaacag
      841 ttccagtgtg cgctcggctt ctgcgcgatc gagtttttgc agcgcgtaac ccacgtgcac
      901 gatcacgtaa tcgccgacgt tcgcatcttc gaccagcgcc agcgagattt ccttgcgcac
      961 gccgccgagg tcgacaatgg cattaccctc cccggtcagc tcctcaatac gtgcgggtat
     1021 ggcaagacac atatcaggct cctttccagt gcgcggcgat ccaggcctgc cccagtgcaa
     1081 ttgcgctatc gcccggcgac atctgctgcg
//
LOCUS       DASRVO010000040         1259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_105050, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000040 DASRVO010000000
VERSION     DASRVO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105050"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFF05_00470"
     CDS             complement(<1..504)
                     /locus_tag="VFF05_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZW50308.1"
                     /translation="MNPADILGGLLAGGLGGLASGFLGVTSGGILVPLLVLILGKDQH
                     VAQGISLVAQVVPTSLSGVRNYSQGGHRISLRWLIWLAVGFGVGGCIGALLATHVSDR
                     ALQWSFVGYLGILLTVVVSRPPRRKRDGSAEVSEDVPLHWAALAAIGAVAGWSSGFLG
                     IGGGLAIT"
     gene            complement(501..>1259)
                     /gene="mazG"
                     /locus_tag="VFF05_00475"
     CDS             complement(501..>1259)
                     /gene="mazG"
                     /locus_tag="VFF05_00475"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZW50309.1"
                     /translation="APYTIEEAYEVTDAIARGDLDDLREELGDLLLQVVFHARMAEEQ
                     NAFAFGDVVEAITRKMIRRHPHVFADKNGRLTPGHVKEAWDRIKAEEKAERAARRPSE
                     EPAHKSLLSDVKAGQPALTRAMELQRKASSVGFDWNDPRAVLHKIREEADEIEAALDR
                     GDSEELADETGDLLFALVNLARHVGADPEAALRGTNAKFERRFGYIERALAAQGRSPE
                     GASLAEMDALWNEAKGKEPSFSSASTGTEGNDRR"
BASE COUNT          232 a          432 c          383 g          212 t
ORIGIN      
        1 ggtgatggcc aatccaccgc cgatgccgag gaatcccgac gaccaaccgg cgacggcccc
       61 gatcgcggcc agcgcagccc aatgcagcgg cacatcttca gacacttccg cggagccgtc
      121 ccgcttccgg cgaggcggcc gcgacacgac gaccgtcaga aggatgccga ggtaaccgac
      181 gaaactccac tgcagggcgc gatctgacac gtgggtagcg agcagggcgc cgatgcagcc
      241 gccaacacca aatccgaccg ccagccaaat cagccagcgc aaggaaatgc gatgaccgcc
      301 ctgactatag ttgcgaacgc cggagaggct ggtcggcacc acctgtgcga ccagcgatat
      361 gccctgcgcg acgtgctgat ccttgccgag gatgagcacc agcaacggca ccagaattcc
      421 gccgctagta actccgagaa agccggaggc gaggccgccc aggccgccgg ccagaagtcc
      481 gcccaaaatg tccgctggat tcaccggcga tcattccctt cagtaccggt cgatgcagac
      541 gaaaagctcg gctccttgcc cttcgcttca ttccacaagg cgtccatctc ggcgagcgac
      601 gcgccctccg gcgaacgtcc ctgcgccgcg agcgcacgct cgatatagcc gaagcggcgc
      661 tcgaatttgg cattggtgcc gcgcagcgcg gcttccggat cggcgccgac atggcgcgcc
      721 agattgacca gtgcgaacaa cagatcgccg gtttcatcgg cgagctcttc gctgtcgccg
      781 cgatccaacg cggcctcgat ctcgtcagct tcctcgcgga tcttgtgcag caccgcgcgc
      841 ggatcgttcc agtcgaatcc gacgctggag gctttgcgct gcaattccat cgcgcgtgtc
      901 agtgcgggtt gcccggcctt cacgtccgac agcagcgatt tgtgcgcagg ttcctctgac
      961 gggcgccgcg ccgcgcgttc ggccttttcc tcggccttga tgcggtccca cgcttccttg
     1021 acgtggccgg gcgtcaaccg gccgttcttg tcggcgaaga catgcggatg ccgccggatc
     1081 atcttgcgcg tgatggcctc gaccacatcg ccgaatgcaa aagcgttctg ttcctccgcc
     1141 atgcgggcat gaaacacaac ctgcagcagg aggtcgccga gttcctcgcg gagatcgtcg
     1201 agatcgccac gcgcgatggc atcggttact tcgtaggctt cctcgatggt gtagggcgc
//
LOCUS       DASRVO010000041         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_53302, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000041 DASRVO010000000
VERSION     DASRVO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53302"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFF05_00480"
     CDS             complement(<1..380)
                     /locus_tag="VFF05_00480"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HZW50310.1"
                     /translation="MRQPSRNSVRGITLIELIFTIVIGSILCAVSLPALGDLMRSGQS
                     CSAHNALVAALNLARSTAASRQSAISICPSTDGAICGNAIWWQHGWIVFADANHDGVR
                     SANEAIIHVGQSHDGVAIASTAGRR"
     gene            530..2569
                     /gene="uvrB"
                     /locus_tag="VFF05_00485"
     CDS             530..2569
                     /gene="uvrB"
                     /locus_tag="VFF05_00485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZW50311.1"
                     /translation="MSDRLQSDPFQLVAPFQPAGDQPEAIEKLVAGFEQGLAHQTLLG
                     VTGSGKTFTIANVIERVQKPTLVLAPNKTLAAQLYGEFKEFFPRNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSINEHIEQMRLSATKALIERRDVVIVSTVSAIYGLGDPGEYH
                     RMVLHLVRGDRLDQRELLRRLTELQYERGDYELHRGAYRVRGDTIDVFPAENEMEAVR
                     IELFDGEIENLSLFDPLTGAVQRKIPRYTIYPGSHYVTTRRTVLEAVETIKVELKERL
                     EQLYAASKLVEAQRLQQRTQFDLEMLAEIGYCNGIENYSRHLTGHAPGEPPPCLFDYL
                     PADALLVVDESHVTIPQLGAMYKGDRSRKETLVEFGFRLPSALDNRPLKFEEWERRAP
                     RCVFVSATPGKYELAHTQGSIVELVVRPTGLVDPQVEVRPVRTQVDDLLGECKLRVAM
                     GDRVLVTTLTKRMAENLTDYLEEHGVRVRYLHSDIETVERVEILRDLRLGKFDVLVGI
                     NLLREGLDMPEVSLVAILDADKEGFLRSTGSLIQTIGRAARNLRGTAILYADRITNSM
                     RAAIDETDRRRQKQVEYNAAHGIVPKSVVKKITDIMEGARADAEAERTGRKSRGGLRV
                     AEEAANYAALAPEKLAAELKRLEAQMYKHAQNLEFEDAARLRDELHRLREAGLRG"
     gene            2647..2721
                     /locus_tag="VFF05_00490"
     tRNA            2647..2721
                     /locus_tag="VFF05_00490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2679..2681,aa:Val,seq:gac)
     gene            2764..>3096
                     /locus_tag="VFF05_00495"
     CDS             2764..>3096
                     /locus_tag="VFF05_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50312.1"
                     /translation="MRRFALYFSLCAGFACATSAFAGVGTTSAGVTTVDPNNPSTDVQ
                     QQSSGFVAANQQAQNAANGGIVLPSGVAGTVQASQNAAGSANAGGAQAGESGTATEHA
                     APAVPPPPP"
BASE COUNT          594 a         1012 c          986 g          504 t
ORIGIN      
        1 cgacggccgg ccgtgctggc gatggccacg ccatcgtgcg actgcccgac atggatgatc
       61 gcctcgttgg cgctgcgcac gccgtcgtgg ttggcatcgg cgaacacgat ccagccgtgc
      121 tgccaccaga tcgcgttgcc gcagatggca ccgtctgtac tcgggcagat cgaaatagcg
      181 gactgacgtg acgcagcggt gctgcgcgcc agattcaacg cggctaccag ggcgttgtgc
      241 gcgctgcacg actgtccgct tcgcatcagg tcgccgagtg cgggcaggct cacggcgcac
      301 aggatggacc cgatcacgat ggtgaagatc agttcgatca gggtgattcc gcggacggaa
      361 ttgcgggatg gctggcgcat ggcgttcctc ctcgacgacg ccgccatgct aggaatcgcg
      421 ccgcatccgc gggatcggcg cgtggcttgg gcatctgtag gaaaacgccg actgcaatat
      481 cgtgaccggg ctataatcag cggttcgctt gcaaagccct tccctcgcaa tgtccgatcg
      541 tcttcaatca gacccgttcc agctcgttgc gccgttccag cctgccggcg accagcccga
      601 ggcgatcgag aaactcgtgg ccggattcga gcagggtctt gcgcaccaga ccctgctcgg
      661 cgtcaccggt tcgggcaaga ccttcaccat cgccaatgtc atcgagcgcg tacagaagcc
      721 gacgctcgtc ctcgcgccga acaagacgct ggccgcgcag ctctacggcg agttcaagga
      781 attttttccg cgcaacgcgg tggaatactt cgtcagctat tacgactact accagcccga
      841 ggcctacgtt ccgtccagcg acacctacat cgagaaggat tcgtcgatca acgagcacat
      901 cgagcagatg cgcctgtcgg cgaccaaggc gctgatcgag cgccgcgacg tggtgatcgt
      961 gtcgacggtg tcggcaattt acggactggg cgaccccggc gagtaccatc gcatggtatt
     1021 gcacctggtg cgcggcgacc gcctcgacca gcgcgaactg ctgcggcgcc tgactgaatt
     1081 gcaatacgag cgcggcgact acgaactgca ccgcggcgcc taccgcgtgc gcggcgacac
     1141 catcgacgtg ttcccggcgg aaaacgagat ggaggcggtg cgcatcgaat tgttcgacgg
     1201 cgagatcgaa aacctgtcgc tgttcgatcc gctcaccggt gcggtgcagc gcaagatacc
     1261 gcgctacacg atctatcccg gctcgcatta cgtgaccacg cgtcgcaccg tgctcgaggc
     1321 ggtggagacg ataaaggtcg agttgaagga acgcctcgag cagttgtacg cggcaagcaa
     1381 gctggtcgag gcgcagcgcc tgcagcagcg cacccagttt gacctggaga tgcttgccga
     1441 gatcggctac tgcaacggca tcgaaaacta ttcgcggcat ctgaccggcc acgcgccggg
     1501 cgagccgccg ccgtgcctgt tcgactatct gcccgccgat gcgctgctcg tcgtggacga
     1561 atcgcacgtg acgattccgc agctcggcgc catgtacaag ggcgaccgtt cgcgcaagga
     1621 aacactagtc gaattcggct tccgcctgcc ctctgcactg gacaaccgcc cgctcaagtt
     1681 cgaggaatgg gaacgccgcg cgccgcgctg cgtcttcgtt tccgcgacgc ccggcaaata
     1741 cgagctggcg cacacgcaag gcagcatcgt cgaactcgtg gtgcgcccga ccggactggt
     1801 cgacccgcaa gtcgaggtgc gcccggtacg cacccaggtc gacgacctgc tcggcgaatg
     1861 caagttgcgc gtggccatgg gcgatcgcgt gctggtgacc actctgacca agcgcatggc
     1921 cgagaacctc accgattacc tcgaggagca cggcgtgcgc gtgcgctacc tacactcgga
     1981 catcgaaacc gtcgagcgcg tggagatcct gcgcgacctg cgcctgggca agttcgacgt
     2041 actggtcggc atcaacctgt tgcgggaagg attggacatg cccgaagtgt cgctcgtcgc
     2101 gatcctcgat gcggacaagg aaggcttcct gcgttcgacc ggctcgctga tccagaccat
     2161 cggccgcgcc gcgcgcaact tgcgcggcac ggcgatcctc tacgccgacc gcatcaccaa
     2221 ctcgatgcgc gcggcgatcg acgaaaccga ccggcgccgg cagaaacagg tggaatacaa
     2281 cgcggcgcac ggcatcgtgc cgaaatccgt ggtcaagaaa atcaccgaca tcatggaagg
     2341 cgcgcgcgcc gacgccgagg ccgagcgtac gggccgcaaa tcgcgcggcg gattgcgcgt
     2401 cgccgaagaa gcagccaatt acgccgcgct ggcgccggaa aaactcgccg ccgaactcaa
     2461 gcgcctcgag gcgcagatgt acaaacacgc gcagaatctg gaattcgagg acgccgcgcg
     2521 cctgcgcgac gaattgcaca gactgcgcga ggcgggattg cgcggctgag atggcgcggc
     2581 gacgtccggc ttggcgcgaa ccggctcggt acggtatcat gcgcgcccct cgggaacatc
     2641 ccctgtgggc agttagctca gcggtagagc actaccttga catggtaggg gtcacaggtt
     2701 cgatccctgt actgcccacc acctcgacgc gttaagatac ggcacctcgt tgcaggaacg
     2761 gccgtgcgtc gattcgctct gtatttttcg ctgtgcgcgg gattcgcctg cgcaacgtcc
     2821 gcattcgccg gtgtcggcac cacatcggct ggcgtgacca cggtcgatcc gaacaatcca
     2881 tccacggacg tgcagcagca gtcgtccgga ttcgtcgcgg cgaaccagca ggcgcagaac
     2941 gcggccaatg gcgggatcgt cctgcccagt ggcgtcgctg gcactgtgca agcgagccag
     3001 aacgctgccg gcagtgcgaa cgcgggcgga gcacaggccg gcgaatccgg cactgcgacc
     3061 gaacacgcgg cacctgccgt gccgccacca ccgccg
//
LOCUS       DASRVO010000042         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_27200, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000042 DASRVO010000000
VERSION     DASRVO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27200"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFF05_00500"
     CDS             <1..506
                     /locus_tag="VFF05_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50313.1"
                     /translation="TFTLAGVIRVDGTMIALFPMPETAFGTCVASNIVATHFPLPPGD
                     GHGWPVAIQFDARTGKAIHVAGDKQAGMPRYTSTIQWLHTPLPAWPTELRRQCMASVW
                     LSLDKDDRVTSAELGDSECPAAFNAALIEDTRQWLGTSRQKATTEDAKDVRVSFAVSA
                     DGIRVTL"
     gene            complement(511..933)
                     /locus_tag="VFF05_00505"
     CDS             complement(511..933)
                     /locus_tag="VFF05_00505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZW50314.1"
                     /translation="MAKADFYLIAKPRFRGDPLLLVCELAKKAFESGQRTLILARSAE
                     QAEQLDEKLWDFDENAFVPHQIAGDEDDAITPVLIVAPGVQTPDRALAINLRDEAVGG
                     TFERVLEVVPDDETQRAGSRERWKAYKTAGIEVLKHDM"
BASE COUNT          183 a          332 c          349 g          194 t
ORIGIN      
        1 cgacgttcac gctggccggc gtgatacgcg tcgacgggac gatgattgcg ctattcccga
       61 tgccggagac ggcctttggc acctgcgtgg cgagcaacat cgtcgcaacg cattttccgc
      121 ttccgcccgg cgatgggcac ggttggccgg tcgccataca gttcgatgca aggaccggca
      181 aggcgatcca cgtggcggga gacaagcagg caggcatgcc gcgatataca tcgacgatcc
      241 aatggctgca cacgccgctg ccggcatggc cgacggaatt gagaaggcag tgcatggcca
      301 gtgtctggct gagcctggac aaggacgatc gggtcacgtc ggccgaactc ggcgactcgg
      361 aatgtccggc ggccttcaat gccgccttga tcgaggacac gcgtcaatgg ctgggcacca
      421 gcaggcagaa agcaacgaca gaggatgcga aggacgtgcg ggtttccttt gccgtgagcg
      481 cagatggcat ccgcgtgacg ctctgagcga tcacatgtcg tgcttgagga cctcgatgcc
      541 ggcagtcttg taggccttcc agcgttcacg cgaaccggcg cgctgggttt cgtcgtcggg
      601 caccacttcc agcacgcgct cgaatgtgcc gccgaccgct tcgtcgcgca gattgatggc
      661 aagcgcgcga tccggcgtct gcacgcctgg cgcgacgatg agtaccggcg tgatcgcgtc
      721 gtcctcgtcg cccgcgatct ggtgcggcac gaaggcgttc tcgtcgaagt cccacaattt
      781 ttcgtcgagc tgttcggctt gttctgccga acgcgcgagg atcagcgtgc gctggccgct
      841 ttcgaacgcc ttctttgcca attcgcacac gagcagcagc ggatcgccgc ggaagcgggg
      901 cttggcgatc agataaaaat cggctttggc catgcgcgcc gtgtgggctt atccggcgcg
      961 attcatcagc cactgcgcga gcagcggcac cgggcgtccg gtcgccgaac ccttgcggcc
     1021 ctcttcccag gcgctgccgg cgatatccag gtgcgccc
//
LOCUS       DASRVO010000043         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_27297, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000043 DASRVO010000000
VERSION     DASRVO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27297"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFF05_00510"
     CDS             <1..204
                     /locus_tag="VFF05_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50315.1"
                     /translation="EMAKCCWYGAPTLINMARYACKRVQHFGNRDADVEGLLPLLYPE
                     THAQSSATGFPDPLSAGQALKGK"
     gene            222..704
                     /locus_tag="VFF05_00515"
     CDS             222..704
                     /locus_tag="VFF05_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50316.1"
                     /translation="MSKLYIRTIYAVSALLLYAMLGGPQLAQAWTMPQQQAQQAQPSA
                     SATTNVPPAQAYPDAPSTQTQPVDAQQTAQAPANDQQNTDTKKKDQNPLGAAAAGAGV
                     TAGGSASRPAGMALAPAKQNRKRSLLIKLGLVAAGAAAIGAVYGLSHATGSTPPGATH
                     "
     gene            721..>1023
                     /locus_tag="VFF05_00520"
     CDS             721..>1023
                     /locus_tag="VFF05_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50317.1"
                     /translation="MKTFLVSFSGLDGAGKTTQIENLRSYLEARGLRTRLLAFWDDVV
                     VLSRYREGFVHKVFKSERGIGAPGKPVNRRDKNVRGWHVNLARHGLYLLDALHLRQV"
BASE COUNT          196 a          319 c          348 g          160 t
ORIGIN      
        1 gagatggcga agtgctgctg gtatggtgcg ccgactctga tcaacatggc gcgctatgca
       61 tgcaagcgcg tgcaacattt tggcaaccgc gatgccgacg tcgaaggcct gctgccgttg
      121 ctgtatccgg agacgcatgc gcagtcttcc gcaaccggtt ttcccgatcc gctttccgcg
      181 ggtcaggcgc tcaaaggtaa atagccccaa ggagttcccc aatgtcgaag ctgtatatcc
      241 ggacgatcta tgccgtttcc gcactgctgc tgtatgcgat gctgggcggc ccgcagttgg
      301 cgcaagcctg gacgatgcca cagcaacagg cgcagcaggc gcaaccgagt gcgtcggcga
      361 ctaccaatgt gccgcccgcg caggcttatc ccgacgcgcc gagcacgcag acgcaaccag
      421 tggacgcgca gcagaccgcg caagcgcccg ccaacgacca gcagaacacc gacacgaaga
      481 agaaggacca gaacccgttg ggcgcggcgg ctgcgggagc aggcgtgacc gcgggtggct
      541 cggcgtcgcg tccggcgggc atggcgctgg caccggccaa acagaaccgc aaacgctcgc
      601 tgctgatcaa gctcggattg gtggcggcgg gcgcggcggc gattggcgcg gtgtacggcc
      661 tgtcgcacgc taccggcagc acgcccccgg gagcgacgca ctaagcagcg gagctgaacg
      721 atgaagacgt tcctggtgag tttcagcgga ctggatggcg ccggcaagac gacgcagatc
      781 gagaacttgc gctcgtacct ggaagcgcgc ggcttgcgca cgagattgct cgcgttttgg
      841 gacgacgtgg tggtgctctc gcgctatcgc gaaggcttcg tccacaaggt gttcaagagc
      901 gagcgcggga tcggagcgcc cggcaagccg gtgaaccgtc gcgataagaa cgtgcgcggg
      961 tggcacgtca atctcgcgcg ccacggtctc tacctgctcg acgcgctcca cctgcggcag
     1021 gtg
//
LOCUS       DASRVO010000044         1405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_287163, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000044 DASRVO010000000
VERSION     DASRVO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287163"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1147
                     /gene="lpxK"
                     /locus_tag="VFF05_00525"
     CDS             218..1147
                     /gene="lpxK"
                     /locus_tag="VFF05_00525"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HZW50318.1"
                     /translation="MLRALSLLFAVLSALRRRLYGMGLLPRVRLPVPVVVVGNITAGG
                     TGKTPLTIALVEALRARGFKPGVVSRGYGGSAREPMLVDAQATPELAGDEAFLIHRAT
                     QAPVAVGRDRAAAARLLLAHGDVDAIVADDGLQHYRLRRDVEICVIDGERRFGNGRLL
                     PAGPLREPMARLASVDFRVCNGGPAQSGEVSMRLAGDTAVALSDPSHKRPLREFAGQR
                     AHAVAGIGNPQRFFASLHAAGIETIEHPFPDHHAFVASDLAFADAAPVLMTEKDAIKC
                     AAFAQENWWCVPVRAELPETFAAAVVANLRGPA"
     gene            1144..>1405
                     /locus_tag="VFF05_00530"
     CDS             1144..>1405
                     /locus_tag="VFF05_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50319.1"
                     /translation="MISNRNPWSWLVPAIAALAAAAFYAAIFYPGTIGFDSAYQWWQA
                     RGGETSNVHGIGMIWFWRLSDAASQGPAFLFMLQLALFWCGLA"
BASE COUNT          218 a          442 c          464 g          281 t
ORIGIN      
        1 cgcgcatcga ctgtcgacca tcgaacacgc cgaccgcatc gccgtgctcg acgccggccg
       61 cattgtcgaa accggcacgc atgccgaatt gctcgcgcgc ggtgggcagt attcactgct
      121 gcacaggatg cagtttcgcg acaatggcgg aaattgattc gtgttcgacg aacgcgaatt
      181 gcaggcggtc tggtatggcg gcaacacacc atctccgctg ctgcgcgccc tgtcactcct
      241 attcgctgtg ctgagcgcac tgcgccggcg actctatgga atgggtttgt tgccgcgcgt
      301 gcgtctgccg gtaccggtcg tcgtcgtcgg caacatcaca gctggcggaa ccggcaagac
      361 gccgctgacg attgcgctgg tcgaagcctt gcgcgcacgc ggcttcaagc ccggcgtggt
      421 cagccgcggc tacggcggat cggcgcgcga acccatgctt gttgatgcac aggcaacgcc
      481 ggaacttgcc ggcgatgaag ccttcctgat ccatcgcgcc acgcaggcac cggtcgcggt
      541 tggccgcgat cgcgcggcgg cggcgcggct gttactcgcg cacggtgatg tggatgcgat
      601 cgtcgccgac gacggtttgc agcactaccg cctgcggcgc gatgtggaaa tctgcgtgat
      661 tgatggcgaa cgtcgcttcg gcaatggtcg cctgctgccg gcgggcccgt tgcgcgaacc
      721 catggcgcgg ctcgcgtccg tggatttccg cgtctgcaat gggggcccgg ctcaatccgg
      781 cgaagtatcg atgcgtctgg ccggcgatac cgccgtggcc ttgtccgatc cgtcgcacaa
      841 gcgtccgctc cgcgagttcg ccgggcagcg cgcgcatgcc gtcgcgggaa tcggcaatcc
      901 gcaacgattc ttcgccagcc ttcacgcggc gggcatcgag acgatcgaac atccgtttcc
      961 ggatcatcat gctttcgtcg cttccgacct tgcgtttgcc gacgccgcgc ccgtgctgat
     1021 gaccgaaaaa gacgcgatca aatgcgccgc gttcgcgcaa gaaaactggt ggtgcgtgcc
     1081 ggtgcgtgcg gaactgcctg agacgttcgc cgcagctgtg gtggcaaatt tgcgcgggcc
     1141 ggcatgattt cgaatcggaa tccatggtcg tggctggtgc cggcaatcgc ggcgctcgca
     1201 gcggcggctt tttatgcggc gattttttat cccggcacca tcgggttcga cagcgcctat
     1261 caatggtggc aggcgcgcgg cggcgaaacc tcgaacgtgc atggtattgg catgatctgg
     1321 ttctggcgct tgagcgatgc cgcatcgcag ggtcccgcgt tcctgttcat gctgcaactc
     1381 gccttgttct ggtgcggatt ggcgc
//
LOCUS       DASRVO010000045         1745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_53425, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000045 DASRVO010000000
VERSION     DASRVO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53425"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1471
                     /locus_tag="VFF05_00535"
     CDS             359..1471
                     /locus_tag="VFF05_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZW50320.1"
                     /translation="MDEGIKFFVGLDVHKDSIAVSVAEAGRAPARLLGSIAHDVAKVL
                     KLLARYRGPESVQVVYEAGPTGFGLQRALARRGYRCHVIAPSLIPQRPGDRVKTDRRD
                     SVRLAELARAGELRAVWIPDPADEAIRDLARAREDAVNARTQVRQQLKGFLLRHDMRY
                     AGKTSWCKTYYRWLATLNFGRAGAQIAFTEYWQAVSAADERVARLTDALANSIAGWRF
                     EAVVQALQALRGIDQVSAIGLVAEIGDIGRFAHPRQLMAYLGLVPSEHSSGDRVARGS
                     ITKTGNAHARRLLTEAAWNYRFQPRIGYRAQRRAEDLPQVLRDLAWKAQLRLTGRFAR
                     LRARGVQINKVCVAVARELAGFVWAIASARAKLPQS"
BASE COUNT          314 a          547 c          594 g          290 t
ORIGIN      
        1 cccagcgcga ctgccagatt gccccacgcc tcggcgtctc ccgggcagcg gcgtgcaagc
       61 tcgcgccatg cccctgcagc ctcggcggcc cgtccggctc ggagcgcgct tcgcccttcg
      121 gcgcgcagcc ggtcgagcaa cggatgggag gggtgtgatg gcgcgcgatt cacggcgatt
      181 ggcccgaacg ggaaacggca agcggtcgac cgctcgcgac atgccgcagc cgatattaga
      241 caatatggtc gcgtcgtaga agcgcgacgg ccggtctccg gcgacgtagg ttgcgcggca
      301 agccgcgccg agctacatgc tgagaacgag tcgtcagcaa ccagaatgga gaccggccat
      361 ggatgagggt atcaagtttt tcgtcggact ggatgtacat aaggactcga tcgcggtatc
      421 ggtagcggaa gccgggcggg cgccggcgcg cttgctgggc agtatcgcgc acgatgtagc
      481 gaaggtgctc aagctgctgg cgcgctaccg tgggccggaa agcgtgcagg tggtctacga
      541 ggccggaccg accggcttcg gcttgcagcg cgcgctggca cggcgtggct atcgctgcca
      601 cgtgattgcg ccctcgttga ttccccaacg accaggcgat cgggtcaaga ccgatcgccg
      661 tgatagcgtg cgcttggccg agctcgcccg cgccggggag ttgcgtgcgg tgtggattcc
      721 cgatccggcc gacgaggcga ttcgcgatct ggctcgcgca cgcgaggatg cggtcaacgc
      781 ccgcacccag gtccggcagc aactcaaagg cttcctgctg cgccacgaca tgcgctatgc
      841 gggcaaaacc tcttggtgca agacctacta ccgctggttg gcgacgttga actttggacg
      901 cgcgggcgcg cagatcgcgt ttaccgaata ctggcaggcg gtcagcgcag ccgacgaacg
      961 cgtcgcacgg ctcaccgacg cgctggcgaa ctcgattgcc ggctggcgct tcgaagcggt
     1021 ggtgcaagcg ctgcaagcgc tgcgcggcat cgatcaggtc agtgcgatcg gcttggtcgc
     1081 cgaaatcggc gacatcggac gcttcgccca cccgcgccaa ctgatggcgt acctgggcct
     1141 ggtgccctcg gagcattcca gcggcgatcg ggtggcccgc ggcagcatta ccaagaccgg
     1201 caatgcgcac gcgcggcgct tgctcaccga ggcggcgtgg aattaccgct tccaaccgcg
     1261 catcggctat cgcgctcagc gccgagccga ggacctgccg caagtgctcc gggacttggc
     1321 gtggaaggcg cagttgcggc ttaccggacg cttcgcgcgg ctgcgcgccc gcggcgtgca
     1381 gatcaacaag gtctgtgtcg cggtggcacg cgaattggcc ggcttcgtgt gggccattgc
     1441 gtcggcgcgg gccaagctgc cgcaaagttg agtcagcgac cacgttcagc aatcagatcg
     1501 atccggtgat gcgacacggg caacgatagg agtaaccctc gagactgcta caccggcatt
     1561 ccgcaaggac tgatccatga cgttagagcg aggcagactc cgcgacgaat cgatgaaagg
     1621 tggtagccaa tccacggata tgagtctgat tcatcgtcgc tgcatcgctc gtgtcgcgtc
     1681 accggatcga tctggagaaa catgaatcac gaccctcttg acgaccgttg cgaccatatg
     1741 agcag
//
LOCUS       DASRVO010000046         1894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_27328, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000046 DASRVO010000000
VERSION     DASRVO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27328"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFF05_00540"
     CDS             <1..816
                     /locus_tag="VFF05_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HZW50321.1"
                     /translation="GGYLVHPVHQQDWGGTVETDLDVEVPKTATVTAQSANGGITIAG
                     VAGPVQVNAANGDIDVHDAGSDVSATVNTGNVRVFNVGGNLRVSGHGSEVDVSGVSGN
                     ASFDGDFFGPVRLRNVAGVTRYSSQRSTLMLAQLTGRLELNPGGIELSNVAGAVKLAT
                     RNKDIEVEDVAGTLEIADSHGDISIHCSRPPSAPIRVSDESGEVDLTLPGSSNFEITA
                     VSQSGEVDSEFQDPSLRLVNDPGVGRLSGTIGSGGPKISIATTYGTISLHKGS"
     gene            complement(897..>1894)
                     /locus_tag="VFF05_00545"
     CDS             complement(897..>1894)
                     /locus_tag="VFF05_00545"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZW50322.1"
                     /translation="NRPMARIAAPLRQMGAAVSGVDGDRAPLEIRGGNLHAIDYTLPV
                     PSAQVKSAILLAGLFADGVTSVHEAVRTRDHTELALREFGASVEIGRGESEPAGPGAP
                     AAANRAEAIVRIHPHPKLQARQIIVPGDLSAAVFLIGAALVLPGSQLLLHNAGLNPTR
                     TRVLDFLIGMGATINVASLQSREGELVGEIAVRHSPLEGGTISGAHVAEMIDELPLLA
                     ALGPYTEKGIEFRDAKELRVKESDRIAALAEGLRRMGAGVEEFPDGLRVEGRASGKLR
                     GAKVDPQGDHRIAMALAVAALGGEGDSTILDSDCAAVSFPEFFSTLAQLRDTGSH"
BASE COUNT          328 a          629 c          609 g          328 t
ORIGIN      
        1 gggggatacc tcgtccaccc cgttcatcag caggattggg gaggcactgt cgagacggac
       61 ctggacgttg aagtgcccaa gaccgcgacc gtcaccgcgc agtctgcgaa tggcggcatt
      121 acgatcgccg gcgtggccgg cccggtgcag gtcaacgccg ccaacggcga tatcgacgtg
      181 cacgacgcgg gatcggacgt ctccgcgacg gtcaacaccg gcaatgtccg cgtgttcaat
      241 gtcggcggga acttgcgcgt cagcggtcac ggcagcgaag tggacgtctc cggcgtttcc
      301 ggcaatgcct ctttcgacgg cgacttcttc gggcccgtcc gcctccgcaa tgtcgctgga
      361 gtgacccgct attcttcgca acgctccacc ctgatgctcg cccagttgac cgggcgtctc
      421 gaactgaatc ccggcggcat cgaattgtcc aacgtggccg gcgctgtcaa gctagccact
      481 cgaaataagg acatcgaagt cgaagatgtt gcgggaacgc tcgaaatcgc cgattcgcat
      541 ggcgacattt ccattcactg ctcgcgtccg cccagcgcgc ccatccgcgt cagcgatgaa
      601 tcgggcgagg tggatttgac cttgcccggc agctccaatt tcgagatcac cgccgtttcg
      661 cagtcgggcg aagtggacag cgaatttcag gatccttcgc tgcgcctggt gaacgaccca
      721 ggcgtcggcc gcttgagcgg caccatcggc tcgggcggcc ccaaaatcag catcgccacc
      781 acctacggca ccatctccct ccacaaaggc agctgacccg ctgcccgaag tcagaaattc
      841 tcgctgctga aaagcaatgg tcggagttcg gtcgaatctg ctgtggacct acgatttcaa
      901 tgcgagccgg tgtcgcgcag ctgcgccaga gtcgagaaga attcggggaa ggagacggcg
      961 gcgcaatcgg agtcgagtat ggtgctgtcg ccttccccgc ccagcgcggc gacggcgagc
     1021 gccatggcga tgcgatggtc gccctgcgga tcgactttcg ctccgcgcag cttcccgctg
     1081 gcgcggccct cgacgcgcag gccgtccgga aactcctcga cgccggcgcc catgcgccgc
     1141 aagccctcgg ccaacgcggc gatgcggtcg ctttctttga cgcgcagctc tttcgcgtcg
     1201 cgaaactcga ttcccttttc cgtatacggc ccgagcgccg ccagcagggg cagctcgtcg
     1261 atcatttccg ccacgtgcgc gccggaaatc gtgccgcctt cgagcggcga gtgccgcacg
     1321 gcaatctcgc cgacgagttc gccttcgcgt gattgcagtg aagctacgtt aatcgtggcg
     1381 cccatgccga tcaggaagtc gaggacgcgc gtgcgcgtgg gattcagccc cgcgttgtgc
     1441 aacagcagct gcgagccggg cagcaccagc gccgcgccaa tcagaaacac tgcggcggag
     1501 aggtcgccgg gcacgatgat ctgccgcgcc tgcaatttcg gatgcggatg aatgcgtaca
     1561 atcgcttccg cgcgattcgc ggcggcgggc gcgccaggac cggcaggctc agattcgccc
     1621 ctgccgattt cgaccgaggc gccgaactcg cgcagggcca gctcagtgtg gtcgcgcgtg
     1681 cgcaccgctt cgtggacgct ggtcacgccg tcggcgaaca gcccggccag cagaatggcg
     1741 gatttcacct gcgcgctggg caccggcagc gtgtagtcga ttgcgtggag atttccgccg
     1801 cggatttcga gcggggcgcg gtcgccatcg acgccgctga ccgcagcgcc catttggcgc
     1861 agcggcgcag caatgcgcgc catgggacga ttgc
//
LOCUS       DASRVO010000047         1433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_261203, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000047 DASRVO010000000
VERSION     DASRVO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261203"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFF05_00550"
     CDS             <1..522
                     /locus_tag="VFF05_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HZW50323.1"
                     /translation="ERAPPEEWLPEVLHDPFDDADEGRRILELLARLSESIGYELETE
                     QYEPILGEIELDDGGAALSAQGWCEGFSRGVDLRARLWEARLGVDSRLTELLSPIMAL
                     AVQEGVFQTDAEIEPLTEAEYDECLAQLPMAIEAVAHYWSESPPTPAELARRAEPVDS
                     ASTPRKRGGHWLH"
     gene            complement(524..1216)
                     /locus_tag="VFF05_00555"
     CDS             complement(524..1216)
                     /locus_tag="VFF05_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HZW50324.1"
                     /translation="MIDLYYWPTPNGHKITLFLEEAELPYTIRRVDIGKGEQFAPEFL
                     AISPNNKMPAIVDHDPPCGGSPLSVFESGAILFYLARKTGKFIAADPRGTVTCLEWLF
                     WQMGGLGPMTGQYGHFNVYAPEKIPYAIERYTREAQRLLGVLDKRLHRREYICGNDYT
                     IADMACYPWIDPYSKAPLDLTPFANVRRWRETIRARPATTRAFAQGDFREAQGEMSEA
                     EHAILFGSKTAA"
     gene            complement(1227..>1433)
                     /locus_tag="VFF05_00560"
     CDS             complement(1227..>1433)
                     /locus_tag="VFF05_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZW50325.1"
                     /translation="FSARTRRVQMRNCPFQRLHVAKEHLEHRTGELAAEELRQAQQAL
                     GEITGQFTSDDLLGKIFSTFCIGK"
BASE COUNT          257 a          437 c          469 g          270 t
ORIGIN      
        1 gaacgcgcgc cgccggagga atggctgccg gaggtcctgc acgatccgtt cgacgacgcc
       61 gacgaaggcc ggcgcatcct cgaactgctc gcgcggcttt cggaaagcat cggttacgaa
      121 ctggaaaccg agcagtacga accgatcctc ggcgaaatcg aactcgacga cggcggcgcg
      181 gcgctaagcg cacaaggttg gtgcgaaggt ttttcgcgcg gcgtcgacct gcgcgcgcga
      241 ctgtgggaag cgcgtctggg cgtcgattcg cggctgaccg aattgctgtc gccaatcatg
      301 gcgctggcgg tgcaggaggg cgttttccag accgacgccg agatcgagcc gctgaccgaa
      361 gccgagtacg acgaatgcct cgcgcaattg ccgatggcca tcgaagccgt ggcgcactac
      421 tggtcggaat cgccgcccac tccggcggaa ctcgcgcgcc gcgccgaacc ggtcgattcc
      481 gccagcacgc cgcgcaaacg cggcggacat tggctgcact gattcatgcg gctgttttgc
      541 taccgaacag gatcgcgtgt tcggcttcgc tcatttctcc ctgcgcctcg cgaaaatcgc
      601 cctgcgcaaa cgcacgcgtg gtcgccggac gcgcgcgaat tgtctcgcgc cagcgccgca
      661 cgttggcgaa cggagtcaaa tccagcggcg ccttgctgta gggatcgatc cacggataac
      721 aggccatgtc ggcaatcgtg tagtcgtttc cgcaaatgta ctcgcgtcgg tgcaagcgct
      781 tgtcgagcac gccgagcagg cgctgcgctt cgcgcgtgta gcgctcgatc gcgtagggaa
      841 tcttttccgg cgcataaacg ttgaagtggc cgtactgacc ggtcatcggt ccaaggccgc
      901 ccatctgcca gaacagccat tccaggcacg tcaccgtgcc gcgcgggtcc gccgcgatga
      961 acttgccggt tttgcgcgca aggtaaaaca ggatcgcgcc cgactcgaac acgctcagcg
     1021 gcgagccgcc gcagggtggg tcgtggtcga cgatcgccgg catcttgttg ttcggcgaga
     1081 tggcgagaaa ctccggtgcg aattgctcgc ccttgccgat gtccacgcgc cggatcgtgt
     1141 agggcaattc ggcttcttcg aggaacaggg tgatcttgtg gccgttgggg gtgggccagt
     1201 agtagaggtc gatcatgttc aatccgtcac ttgccgatgc aaaacgtgga aaagattttt
     1261 ccaagcaaat cgtcgctggt gaattgtccg gttatttcgc ccagcgcctg ctgtgcctga
     1321 cgcaattcct cggcagcgag ttcgccggtg cggtgttcga gatgctcttt cgccacgtgc
     1381 aggcgctgga agggacagtt gcgcatctgc acgcggcggg tgcgcgcgct gaa
//
LOCUS       DASRVO010000048         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_105176, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000048 DASRVO010000000
VERSION     DASRVO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105176"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VFF05_00565"
     CDS             complement(<1..1130)
                     /locus_tag="VFF05_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HZW50326.1"
                     /translation="MKKPVFFLLAALAGCAQVATQPPTQPSTPSNADAVQADAAQATV
                     AHFDAIRHSPPQLEAFLRAMPKGADLHNHLSGAIYAEDLLNWAAQDGLCIERATDTAV
                     VPPCDAAQGRPLAAAAFGDDALYRQVIDAWSMRDFVPSSGESGHDHFFDTFGKFHLVT
                     RAHRGDMLARAVHQAAHDREVYIELMDTLAEAQLRSLVARTPWDGDVAHAHAALQPGM
                     PAILAEAKNELDSAQRQMREELRCGSAQADPGCGVEVRFLYQVLRGFPAQRVFAQMLT
                     GFELATSDARVVGINMVMPEDGYLSMRDFREQMAMLDYLHAQFPQVHISLHAGELSST
                     LVPPDGLRFHIRASIEQGHAQRIGHGVDVMHEDRPQELLREMA"
     gene            complement(1164..>1900)
                     /gene="gspK"
                     /locus_tag="VFF05_00570"
     CDS             complement(1164..>1900)
                     /gene="gspK"
                     /locus_tag="VFF05_00570"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HZW50327.1"
                     /translation="VRAQDKGTSYDAADSVWAIPLPPTPVPGGTIAAQMSDFNGRFNL
                     NNLDPAYDTQGVWSGKFVLLLTALKLDPAIAGHIVTWMDANAAASADDQFYLAQSVPY
                     RRAGRAFTHVSELRLVAGVDGDVYAALAPYVAALPEGTPINVNTASVPVLMTLGANVT
                     REMAQAIWQQGGAHYTGVDEVLKAQPALGAIEHPECYDVRSSYFRARGLVTLDGLPFE
                     FDSLIERRSGVGGGIRVLRRSRGGDD"
BASE COUNT          342 a          621 c          610 g          327 t
ORIGIN      
        1 gccatctcgc gcagcaattc ctgcggacga tcctcgtgca tgacgtccac gccatggccg
       61 atgcgctgcg catgaccctg ctcgatcgag gcgcggatgt ggaagcgcaa gccatccggc
      121 ggcaccaatg tcgaggacag ctcgccggca tgcagggaaa tgtgtacttg tggaaactgc
      181 gcgtgcaggt aatcgagcat cgccatctgt tcacggaaat cgcgcatcga caggtaaccg
      241 tcttcgggca tgaccatgtt gatgccgacc acgcgtgcgt cgctcgtggc cagctcgaag
      301 ccggtgagca tctgcgcgaa gacgcgctgc gccggaaaac cgcgcagcac ctggtacagg
      361 aaacgcactt ccacgccgca gccgggatca gcctgcgcgc tgccgcagcg cagctcctcg
      421 cgcatctggc gttgcgctga atccagttca ttcttcgctt ccgccagtat ggccggcatg
      481 cccggttgca aggccgcgtg cgcatgtgca acgtcgccat cccacggcgt gcgcgccacc
      541 agggaacgca gttgtgcttc ggccagcgta tccatcaatt cgatgtaaac ctcgcggtcg
      601 tgcgcggcct gatgcacggc gcgcgcgagc atgtcgccgc gatgcgccct ggtgaccaga
      661 tgaaacttgc cgaacgtatc gaagaaatga tcgtgcccgg attcgccgct gctcggcacg
      721 aaatcgcgca tcgaccaggc gtcgatgacc tggcggtaca acgcgtcgtc gccgaatgcg
      781 gcggcggcca acggccggcc ttgcgcggcg tcgcagggcg gtacgaccgc cgtgtccgtc
      841 gcacgttcga tgcaaagacc atcctgcgcc gcccaattca gcaggtcttc ggcgtagatc
      901 gcgccggaaa ggtgattgtg caagtccgca cccttgggca tggcgcgcag gaacgcttcc
      961 aactgcggcg gcgaatggcg gatcgcgtcg aaatgtgcga ccgtcgcctg tgcggcatcg
     1021 gcctgcacgg catcggcgtt ggatggcgtg gatggctgcg tgggcggttg cgtcgccacc
     1081 tgcgcgcaac cggcgagcgc ggcgagcaga aaaaatacgg gcttcttcat cgcgggagga
     1141 tacgtcatcg cccccggtgg acgtcaatcg tcgccgcccc ggctgcgccg taaaacgcgg
     1201 attcccccac ccaccccgct gcgccgttcg atcaggctgt cgaattcgaa cggcagtccg
     1261 tcgagggtca cgaggccgcg cgcgcggaag tagctgctgc gcacgtcgta gcattcggga
     1321 tgttcgatcg cgccgagcgc cggctgcgcc ttcaacactt cgtccacgcc agtgtagtgc
     1381 gcaccaccct gctgccagat cgcctgcgcc atttcacgcg tcacgttcgc gcccaacgtc
     1441 atcagcaccg gcacactggc ggtgttcacg ttgatcggcg tgccttcggg caaggcggcg
     1501 acgtagggtg cgagcgcggc atagacgtcg ccatcgacgc ctgcgacgag acgcaattcc
     1561 gacacatgcg tgaatgcgcg gcccgcgcgc cggtagggca cggactgggc gagatagaac
     1621 tgatcgtccg cactcgctgc cgcattcgca tccatccagg tgacgatgtg accggcaatc
     1681 gcgggatcaa gtttgagcgc ggtcagcaac agcacgaatt tgccgctcca gacaccctgc
     1741 gtatcgtaag ctggatcgag attattgaga ttgaagcggc cattgaaatc ggacatctgc
     1801 gccgcgatcg tgccgccggg caccggtgtg ggtggcaacg ggatcgccca gacgctatcg
     1861 gcggcgtcat agctcgtgcc cttatcctgc gcgcgcacca
//
LOCUS       DASRVO010000049         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_131079, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000049 DASRVO010000000
VERSION     DASRVO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131079"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..315
                     /locus_tag="VFF05_00575"
     CDS             121..315
                     /locus_tag="VFF05_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50328.1"
                     /translation="MLATAAVMTLNPLFLRHDLMIEMSRLEMAIENARHHGSANDADA
                     VSPLETKLARINEALSRLSA"
     gene            complement(385..1968)
                     /locus_tag="VFF05_00580"
     CDS             complement(385..1968)
                     /locus_tag="VFF05_00580"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZW50329.1"
                     /translation="MLLGGSPHGVWLWRWLAVLSGALSLVGLFDMGQRKHTLYRNFPL
                     TAHIRTFFEFFRPMLRQYVVESDTEEVPFSHAQRALVYQRAKNVEDKRPFGSEQNLYA
                     TQYEWINHSLAPAKIEGHDFRITIGGPECAQPYSASVFNISAMSFGSLSANAIRALNE
                     GARRGGFYHDTGEGSLSRYHREMGGDICWEIGSGYFGCRNDDGSFSLDKFVENATNPQ
                     VKMIEIKLSQGAKPGHGGVLPAAKVSAEIAEARGVPMGVDCVSPAAHSAFSTPLELLQ
                     FVARLREKSGGKPTGFKLAIGHPWEWFGIAKAMQESGLLPDFIVVDGGEGGTGAAPLE
                     FADHVGAPLREALLLVHNTLVGLNLRDKVRLGASGKIITGFDLARTLALGADWCNAAR
                     GFMFALGCIQSLNCHTDRCPTGIATQDPSRWKKLDVPDKAERVRMFHDNTLKGLKELI
                     AASGLSHPNEIGPEHIIRRVSSTEVRSLSALYHFVKPGELIGGLPDHAVFRVFWNAAR
                     TDSLAPPKYVLEQHHTKVR"
     gene            2174..3100
                     /gene="ubiA"
                     /locus_tag="VFF05_00585"
     CDS             2174..3100
                     /gene="ubiA"
                     /locus_tag="VFF05_00585"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HZW50330.1"
                     /translation="MPRPALAPTRAGIDWPRVRARLIEYAKLARMHRPVGALLLLWPT
                     WWALWLATGDFPAIGLLVVFTLGVWLMRSAGCVVNDLADRKFDPQVKRTRERPLAAGT
                     VAPREAVGVFIALIALAFGLVLLTNKLTIELSFIGAFLAVSYPFMKRYTYLPQVYLGC
                     AFGWSIPMAFAAVTGSWDTFVKMLPLCGLLFLANVLFATIYDTEYAMVDRDDDIRIGV
                     KSTAILFGDADLPILGILMVTFLGAMALVAQRGNLHWIYFVGVGIAALMFAWQLWIMR
                     RRERDACFAAFRSNNWVGLALWLGVLLGYALR"
     gene            complement(3085..3789)
                     /locus_tag="VFF05_00590"
     CDS             complement(3085..3789)
                     /locus_tag="VFF05_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZW50331.1"
                     /translation="MRRLQFAVLPPHCLFCGLSGAGQRDLCTACAADLLCNRVCCPRC
                     ALPLEAPAPLCGECLHSEPPFASACVPFVYAPPLDALLTHLKFGRSLAAGRVLSELWI
                     TAARASLPSIPQALIPVPLHAGRLRERGYNQALELAKPLARAFGVALRNDVLVRTRVT
                     SAQSNLDADARHRNLRGAFVVAPDATLPAHVALFDDVMTTGTTLRECARTLLRAGAER
                     VDVWAVARAPKRYLSA"
BASE COUNT          659 a         1246 c         1206 g          680 t
ORIGIN      
        1 ttgccgtcaa ctttcgtcaa aaaacgtcac cggcttgcgc gaatcccggg gaaatggcgt
       61 gttttctagg ttggcacggc ttctgcatta tccaaatcac atcgcagatt ggagagtgcc
      121 atgctcgcta ccgccgccgt aatgaccttg aatccgctgt tcctgcgcca cgatctgatg
      181 atcgaaatga gccgcctgga aatggccatc gagaatgcgc gtcaccacgg ttccgcgaac
      241 gatgccgatg cggtcagccc gctggaaacc aagcttgcgc gcatcaacga agcgctgagc
      301 cgtctctccg cctgatccga atactaccga actccctacg ccccgggacg gatgcgagcc
      361 gcccggggtt ttttttgccc gcgctcagcg caccttggtg tggtgttgtt ccagcacgta
      421 cttcggcggc gcgagactgt cagtgcgcgc cgcattccag aacacgcgga acacggcgtg
      481 atccggcaat ccgccgatca gctcgccggg tttgacgaaa tggtatagcg ccgacagcga
      541 gcgcacttcc gtcgacgaca cgcgccggat gatgtgttcc gggccgatct cgttcggatg
      601 gctcaagccc gaggcagcga tcagttcctt cagccccttg agcgtattgt cgtggaacat
      661 gcgcacgcgt tcggctttgt ccggcacgtc gagtttcttc cagcgcgatg ggtcctgcgt
      721 ggcgatgccg gtcgggcaac ggtcggtatg gcaattgagc gactggatgc atccgagcgc
      781 gaacatgaag ccacgcgcgg cgttgcacca gtccgcgccg agtgccaggg tgcgcgccag
      841 atcgaagccg gtgatgatct tgccgctggc gcccaggcgc accttgtcgc gcaggttcag
      901 gccgacgagc gtgttgtgca cgagcagcaa cgcttcgcgc aacggcgcgc cgacgtgatc
      961 ggcgaattcc agcggcgctg cgccggtgcc gccctcgcca ccgtcgacga cgatgaagtc
     1021 cggcagaagt ccggattctt gcatggcctt ggcgatgccg aaccattccc acgggtggcc
     1081 gatcgcaagt ttgaagccgg tcggtttgcc gccggatttc tcacgcaggc gggcgacgaa
     1141 ttgcagcagt tctagcggcg tcgagaacgc gctgtgcgct gccggcgaga cgcagtccac
     1201 gcccatcggc acgccgcgcg cctcggcgat ttccgcgctc accttggccg ccggcagcac
     1261 gccgccgtgg cccggtttcg cgccttgcga gagtttgatc tcgatcattt tcacttgtgg
     1321 atttgtggca ttttccacaa atttatcgag actgaaactg ccgtcgtcgt tgcggcagcc
     1381 gaaatagccg gagccgattt cccagcagat gtcgccgccc atctcgcggt gatagcgcga
     1441 caacgatcct tcgccggtat cgtggtagaa accgccgcga cgcgcgcctt cgttcaacgc
     1501 gcggatcgcg ttcgccgaga gcgaaccgaa actcatcgcc gagatgttga acacgctggc
     1561 cgaatacggc tgcgcgcatt ccggcccgcc gatcgtgatg cggaaatcgt ggccttcgat
     1621 cttcgcgggt gcgagcgaat gattgatcca ctcgtattgc gtcgcgtaca ggttctgctc
     1681 cgaaccgaac gggcgcttgt cctcgacgtt cttcgcacgc tggtaaacga gagcgcgctg
     1741 ggcatgcgaa aacggcactt cttcggtgtc cgattcgacc acgtactggc gcagcatcgg
     1801 ccggaagaat tcgaagaacg tgcggatatg cgcggtgagc gggaaattgc ggtacagcgt
     1861 gtgcttgcgc tgccccatgt cgaagaggcc gaccagggat aatgcgccgc tcagcaccgc
     1921 cagccagcgc cagagccaca cgccatgcgg cgatccgccc aacagcatgg ccagcgacaa
     1981 cgcgagcacg gccacgcaca acacgaacgg catgaaccgg gacggcagca gtttttccat
     2041 cacgcacccc ctgaggaaat gtccgcgcag gatacgccgc gcgcaatcgc atcgctatcg
     2101 ttacactagc cgcatgcccg caaaatcgcc tcccccgcca cgcccaccga tacgccccgc
     2161 tgttgcgcgc gccgtgccgc ggcccgcgct cgcgccgaca cgtgccggca tcgattggcc
     2221 acgcgtgcgt gcgcgcctga tcgaatacgc caagctggca cgcatgcacc ggccggtcgg
     2281 cgcgctgctg ctgctgtggc cgacgtggtg ggcactgtgg ctggcgaccg gcgacttccc
     2341 cgcgatcgga ttgctggtcg tcttcacgct cggcgtatgg ctgatgcgct cggccggctg
     2401 cgtggtcaac gacctcgccg accgcaagtt cgatccgcag gtcaagcgca cgcgcgagcg
     2461 cccgctggcc gccggcacgg tcgcgccgcg cgaggccgtc ggcgttttca tcgcgctgat
     2521 cgcgctcgcc ttcggactcg tgctgttgac caacaagctc acgatcgagc tgtccttcat
     2581 cggcgcgttc ctcgccgtga gctatccgtt catgaaacgc tacacctacc tgccgcaggt
     2641 gtacctgggc tgcgcgttcg gttggtcgat cccgatggcg ttcgcggccg tcaccggaag
     2701 ctgggacacc ttcgtcaaga tgctgccgct gtgcggcctg ctgtttttgg caaacgtgct
     2761 gttcgcgacg atctacgaca ccgagtacgc gatggtcgac cgcgatgacg acatccgcat
     2821 cggcgtaaaa tccacggcga tcctgttcgg cgacgcggac ttgccaattc tcgggatttt
     2881 gatggtcacc tttctcggtg cgatggcgct ggtggcgcag cgcggaaatc tgcactggat
     2941 ctacttcgtc ggcgttggca tcgccgcgct gatgttcgcc tggcagctgt ggatcatgcg
     3001 ccggcgcgag cgcgacgcgt gttttgcggc gttccgcagc aacaactggg ttggattagc
     3061 cctttggctg ggcgtgctac tgggttacgc gctcaggtag cgtttcggcg cgcgcgctac
     3121 cgcccacaca tccacgcgtt ccgcgcccgc gcgcagcagc gtgcgtgcac attcgcgcag
     3181 ggtcgtgccg gtggtcatca cgtcgtcgaa caacgcgacg tgcgcgggca gcgtggcatc
     3241 cggcgcgacc acaaatgccc cgcgcagatt gcgatggcgc gcatcggcat ccagattcga
     3301 ctgtgccgac gtgacccgcg tccgcacgag aacgtcgttg cgcagcgcga cgccgaacgc
     3361 gcgcgccagc ggcttcgcca gttccagcgc ctggttgtaa ccgcgctcgc gcaggcgacc
     3421 ggcgtgcagc ggcaccggaa tcagcgcctg aggaatcgac ggcagcgatg cacgcgcagc
     3481 cgtaatccac agctcggaca agacgcgtcc ggcggcgaga ctacggccga atttcaagtg
     3541 cgtaagcagc gcatccagcg gtggcgcata gacgaacggc acgcaagccg atgcgaacgg
     3601 cggttcgctg tgcaggcact cgccgcacag cggcgcgggc gcctccagcg gcagcgcgca
     3661 gcgcgggcag cacacgcgat tgcacaacag gtctgcagcg caggccgtgc acaggtcgcg
     3721 ctgtccggcg ccggacagac cgcagaacag acaatgcggc ggcagcacgg cgaattgcag
     3781 gcgacgcagg c
//
LOCUS       DASRVO010000050         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_313102, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000050 DASRVO010000000
VERSION     DASRVO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313102"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFF05_00595"
     CDS             <1..684
                     /locus_tag="VFF05_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW50332.1"
                     /translation="HPELGMISPGFFIPLAEDNGLIVPIGEWVMRTACADARRWQDRF
                     ALNLRVSVNLSALQLRQQNLVEVVAQVLRDTGLEANLLDLEVTESINVKNIPNLLETL
                     QGLRDLGCHIAIDDFGTGQSSLDYIKRFPADRIKIDQTFVRNIGVDPDDEAIVRATIG
                     MAHNLGRAVVAEGVEIEQHLDFLRGEGCEELQGYLFCRPLPAASFENLLSERERLFQN
                     AQQAGETAA"
     gene            765..1361
                     /gene="rpoE"
                     /locus_tag="VFF05_00600"
     CDS             765..1361
                     /gene="rpoE"
                     /locus_tag="VFF05_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HZW50333.1"
                     /translation="MGDNESDLALVERVQKGEKRAFDLLVRKYQHKVIGVISRYVSDW
                     SECQDVAQEAFMRAYRAMAAFRGDSQFYTWMYKIATNTAKNHLVSQGRRPPADDIGID
                     DAVQLDGGMRLKDRATPEHELMRQEIEQTVFDTVEQLPEDLKMAITLREVDGLSYEEI
                     AEKMDCPIGTVRSRIFRARDAIDQKLRPLLSDAKVSES"
     gene            1358..>1669
                     /locus_tag="VFF05_00605"
     CDS             1358..>1669
                     /locus_tag="VFF05_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HZW50334.1"
                     /translation="MSQVLREQLSALMDGELPKDQMRFLLRGLDADAQLAQSWSRYQL
                     ASAVIRREAAFVPVDAGFADRVMLAVSQQTAPGRHGVRILRWAGGGAIAAAVAVIALV
                     GT"
BASE COUNT          325 a          505 c          554 g          285 t
ORIGIN      
        1 catccggaac tgggcatgat cagtcccggg tttttcattc cgctggccga ggacaacggc
       61 ctgatcgtgc cgatcggcga gtgggtgatg cgcaccgcct gtgcggatgc acgccgctgg
      121 caggaccgct tcgcgctcaa cctgcgcgtc agcgtgaacc tgtccgcctt gcaattgcgc
      181 cagcagaatc tggtcgaagt cgtggcgcag gtgctacgcg acacgggcct ggaagcgaac
      241 ctgctcgacc tggaagtcac cgagagcatc aacgtcaaga acatcccgaa cctgctcgaa
      301 accctgcagg gcctgcgcga cctcggctgc cacatcgcga tcgacgattt cggcacgggc
      361 cagtcgtcgc tggattacat caagcgcttc ccggccgacc gcatcaagat cgatcagacc
      421 ttcgtgcgaa acatcggcgt ggatccggac gacgaagcga tcgtgcgcgc caccatcggc
      481 atggcgcaca atctcggccg cgccgtggtc gcggaaggcg tggagatcga gcagcacctg
      541 gatttcctgc ggggggaagg ctgcgaggaa ctgcagggct acctgttctg ccggccgttg
      601 ccggcagcca gcttcgaaaa cctgctgagc gagcgcgaac gcctgttcca gaatgcccag
      661 caggcgggcg aaacggccgc atgaaagaac ccgtgaactt gttggcctca ggtttgtcta
      721 tcagcccggc gtgaccggga cgccagtaca ggagtaatgc ccggatgggc gacaacgagt
      781 cggatctggc cctggtcgaa agggtgcaga aaggagaaaa gcgcgcgttc gacctgctgg
      841 tgcgcaagta ccagcacaag gtgatcggcg tgatttcgcg ctacgtttcg gactggagcg
      901 aatgccagga cgtggcgcag gaagcgttca tgcgcgcgta tcgcgccatg gccgcgtttc
      961 gtggcgacag tcagttttat acgtggatgt acaagatcgc gaccaacacc gccaagaacc
     1021 atctcgtctc gcaggggcgc cggccgcctg ccgacgacat tggcatcgac gacgccgtgc
     1081 agctcgacgg cggcatgcgc ctgaaggacc gcgccacgcc cgaacacgaa ctgatgcgcc
     1141 aggaaattga acaaaccgtg ttcgacacgg tcgaacagtt gcctgaagac ctgaagatgg
     1201 cgattacatt gcgcgaggtc gacggcctga gttacgagga aatcgccgaa aagatggatt
     1261 gcccgatcgg caccgtgcgc tcccgcatct tccgggcgcg cgatgcgatc gaccagaagc
     1321 tgcgcccgtt gttatcggac gccaaggtga gcgaatcatg agccaggtac tgcgcgaaca
     1381 actctccgcc ctgatggacg gcgaactgcc gaaagaccag atgcgttttc ttctgcgcgg
     1441 tctggatgcg gatgcgcagc tggcgcagag ctggtcgcgc taccagttgg ccagtgcggt
     1501 gatccggcgc gaggccgcgt tcgtgccggt cgatgccggt ttcgccgacc gcgtcatgtt
     1561 ggcggtatcg cagcagactg cgccgggcag gcatggcgtg cgcatcctgc gctgggccgg
     1621 cggtggtgcc atcgccgcgg cggtggcggt gatcgcgcta gtcggtacg
//
LOCUS       DASRVO010000051         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_131169, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000051 DASRVO010000000
VERSION     DASRVO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131169"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VFF05_00610"
     CDS             <1..1161
                     /locus_tag="VFF05_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZW50335.1"
                     /translation="DPLDLIDGIELEALVKKSTASLLIPQVREKVEKRIRKEYPLGIA
                     PVGADALRFTFAALATYGRTINFDLKRAEGYKNFCNKLWNAARFVLMNCEGFSTPAGT
                     KLAPKTDAEKWILARFAQTLAEVEAQMPLYRFDLVAQALYEFTWNEFCDWFVELAKPA
                     LNGDDKVIADSTRHTLLAALEALLRALHPIIPFITEEIWHEVAPKLGIAGDSISTQAY
                     PQSSQVAVDAAAEAETEWLKAVVSSVRGIRSQMNIAPGKSIPILYASGVATDRVRSAK
                     FADAIAFLARAESQRWLATGEAEPASAAAIIGEMKILIPLAGLIDVDAEKARLAKEIA
                     RLDGENTKSNAKLANFGPNTPAAVVEQETQRVAERTAQLAALREQVQRLAAM"
     gene            1232..1522
                     /locus_tag="VFF05_00615"
     CDS             1232..1522
                     /locus_tag="VFF05_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZW50336.1"
                     /translation="MAATTTSLKLPAEVRTRALAAARREGVTPHAFMVEAIRTASDAA
                     AKRAEFIAQAVAARRDALRSGKGYAADDVHAYLRQRLRGAKVRKPGLKAWRG"
     gene            1510..1806
                     /locus_tag="VFF05_00620"
     CDS             1510..1806
                     /locus_tag="VFF05_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZW50337.1"
                     /translation="MARLIYTRRALADLERLVDFLLDADPATAQDTGALIIEAVQILA
                     NHPLVGRPAEDDLRELVISRGKSGYLALYSYQAVPDAVLILAVRHQRETGYVQD"
     gene            complement(1797..>2165)
                     /gene="rimI"
                     /locus_tag="VFF05_00625"
     CDS             complement(1797..>2165)
                     /gene="rimI"
                     /locus_tag="VFF05_00625"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZW50338.1"
                     /translation="RDCLRAGYDCIVLAHGIEVVGYGVLSAAAGEAHVLNVCVAPAQQ
                     GQGHARRLMKRLIDLARWHRAERIFLEVRPSNTHAIALYDKLGFNEIGKRPNYYPAKR
                     GREDAVVMAMELLPPDEPQS"
BASE COUNT          416 a          685 c          690 g          374 t
ORIGIN      
        1 gacccgctgg acctgatcga cggcatcgag ctcgaagcgc tggtgaagaa atccaccgcc
       61 tcgctgctga ttccgcaagt gcgcgagaaa gtggaaaagc gcatccgcaa ggaatatccg
      121 ctgggcatcg cgccggtcgg cgccgatgcg ctgcgcttca ccttcgccgc gctggcgacc
      181 tatgggcgca cgatcaattt cgatctcaag cgcgccgagg gctacaagaa cttctgcaac
      241 aagctgtgga acgcagcgcg cttcgtgctg atgaactgcg aagggttcag cacgccggcg
      301 ggtacgaaac ttgcgccgaa gacggatgcg gaaaagtgga ttttggcgcg ttttgcccag
      361 accctcgccg aagtggaagc gcagatgccg ctgtaccgct tcgacctcgt cgcgcaggcg
      421 ctgtacgaat tcacctggaa cgaattctgc gactggttcg tggagctcgc caagccggcg
      481 ctgaacggcg acgacaaggt catcgccgat tccacgcgcc acaccctgct ggccgcgctc
      541 gaagcgctgc tgcgcgcgct gcatccgatc atcccgttca tcaccgagga aatctggcac
      601 gaagttgcgc caaaacttgg gattgccggc gattcgattt cgacgcaagc gtatccgcag
      661 tcatcgcaag tcgctgtcga tgcggccgcg gaagcggaaa ccgagtggct caaggccgtg
      721 gtttcgtcgg tgcgcggcat ccgtagccag atgaatatcg cgccgggaaa atcgattccg
      781 attctgtatg cgagcggcgt tgcgaccgat cgcgtgcgct cggcgaagtt cgcggatgcc
      841 atcgcattcc ttgcgcgcgc cgaatcgcaa cgctggctgg cgaccggcga agccgaaccg
      901 gcgtccgccg ccgccatcat cggcgaaatg aaaatcctca ttccgctcgc cggactgatc
      961 gacgtcgacg cggaaaaagc gcggctggca aaggaaatcg cgcgcctcga tggtgaaaac
     1021 acgaagtcca acgccaagct cgcgaacttc ggcccgaaca cgccggccgc cgtcgtcgaa
     1081 caggaaacgc agcgcgtcgc cgaacgcacc gcgcaactgg ctgcactgcg cgagcaggtg
     1141 cagcggctgg cggcgatgta gatttgcgcg ggactagcgc cgacgacttt caggtgttac
     1201 ttttgtagca tctattccgg aagcgttcga aatggccgca accacgacat cgctgaaact
     1261 ccctgccgaa gttcgcacgc gggcactcgc ggccgcccgg cgcgagggcg tcaccccaca
     1321 tgccttcatg gtcgaggcaa ttcgcaccgc gtcggacgcc gcggcaaaac gggccgagtt
     1381 catcgcgcag gcggttgccg cgcggcgcga tgccttgcgc tcgggcaagg gttacgcggc
     1441 agacgacgtt catgcgtatt tgcgtcagcg tctgcgcggt gcgaaagtac gcaagccggg
     1501 actcaaggct tggcgcggct aatatacacg cgccgggcac tcgcggacct ggagcgactc
     1561 gtcgatttcc tgctcgatgc cgatccggcg actgcgcagg ataccggtgc gctgatcatc
     1621 gaagccgtgc aaattctggc aaaccacccg ctggtgggac gtcctgcaga agacgatctg
     1681 cgcgaactcg tgatctcgcg cggaaagtcc ggttatctcg ccctctacag ctaccaggcc
     1741 gtacccgacg ccgttctgat tctggcggtg cggcatcagc gtgaaacggg atatgttcag
     1801 gattgaggct cgtcgggcgg caacaactcc atcgccataa ccaccgcgtc ctcgcggccg
     1861 cgcttggccg ggtagtagtt cgggcgcttg ccgatttcgt tgaagccaag cttgtcgtac
     1921 aacgcaatgg cgtgtgtatt ggacgggcgc acttcgagga agatgcgctc ggcgcgatgc
     1981 cagcgcgcca ggtcaatcag gcgtttcatc aggcgtcgcg cgtggccctg gccttgctgc
     2041 gccggcgcga cgcagacgtt gagcacgtgc gcttcgcccg cggcggcact cagcacgccg
     2101 tagccgacca cctcgatgcc gtgcgcgagc acgatgcagt cgtaaccggc gcgcaggcaa
     2161 tcgcg
//
LOCUS       DASRVO010000052         1442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_105302, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000052 DASRVO010000000
VERSION     DASRVO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105302"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFF05_00630"
     CDS             complement(<1..764)
                     /locus_tag="VFF05_00630"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HZW50339.1"
                     /translation="MRVLRTCAAILVVTALTLGIAWADAGMQPIPPFGARVTDLTHTL
                     SAEQVQSLTQQLQALEQRKGSRVAILMLSTTQPEDIAQYSIRVTDAWKLSRTDKQAQD
                     GVLLTIAKDDHRVRIDVGRDLEGAIPDAAADRIIREYITPKFRAGDFNGGITDALGAL
                     TKLIDGEQLPPPLTDNRHGQSPGDAFNLLVPLIFAVLWMRVFLGRLPSAPRAGLIGAI
                     CGGAVWLITGVAAFAVGAGVIGLLLGWLGGSGGGFAG"
     gene            complement(768..1406)
                     /locus_tag="VFF05_00635"
     CDS             complement(768..1406)
                     /locus_tag="VFF05_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZW50340.1"
                     /translation="MPMRLQRLVILALAAFMLAGCGYNAIQSKDEAVKAAWSEVINQY
                     QRRADLVPNLVNTVKGYAEHEQSTLEAVTNARAQVGKITLTTDDLNDEAKMKAFQQAQ
                     GELSGALSRLMAVSENYPNLKADSLFQTLQAQLEGTENRIAVARNRYISSVQDYNVLV
                     RSFPQNLTAKMFGYSTKANFTVENEKQISTAPTVDFGKPAAPPAQPPKQPQN"
BASE COUNT          239 a          452 c          481 g          270 t
ORIGIN      
        1 ccggcaaaac cgccgccgct gccgccgagc cagccgagca gcaacccgat cacacccgca
       61 ccgacggcga acgcggcgac gcccgtgatc agccacaccg cgccgccgca aatcgcgccg
      121 atcagcccgg cacgcggcgc agacggcagg cgccccagaa agacccgcat ccacaatacc
      181 gcgaagatga gtggaaccag gagattgaac gcgtcgccgg gggattggcc atgccgattg
      241 tcggtgagtg gcggcggcaa ttgctcgccg tcgatgagtt tcgtcagcgc gccaagcgca
      301 tccgtgatgc cgccgttgaa atcgccggcg cggaatttcg gcgtgatgta ttcgcgaatg
      361 atacggtcgg cggcggcatc ggggatcgcg ccttccaggt cgcgaccaac gtcgatgcgc
      421 acgcgatgat cgtccttggc gatggtcaga agcacaccat cctgggcctg cttgtcggtg
      481 cgcgagagtt tccacgcatc ggtcacgcgg atgctgtact gggcgatgtc ctcgggttgc
      541 gtcgtcgaca acatgaggat cgcgacgcgt gaacccttgc gctgttccag cgcctgcagt
      601 tgctgggtga gcgactgcac ctgttcggcc gagagcgtgt gcgtgaggtc cgtgacccgc
      661 gcgccgaacg gcggaatcgg ctgcatgccg gcatcggccc acgcaatgcc cagcgtcagc
      721 gcagtgacca cgaggatcgc cgcgcatgta cgcaacacgc gcatgcgtca gttctgcggc
      781 tgcttgggcg gctgggccgg tggcgcggcg ggcttgccga aatccaccgt cggcgcggtg
      841 gaaatctgct tctcgttctc caccgtgaag ttcgccttcg tcgaatagcc gaacatcttc
      901 gcggtgaggt tctgcgggaa gcttcgcacc agcacgttgt aatcctgcac cgaggaaatg
      961 tagcgattgc gcgccaccgc gatgcggttc tcggtgcctt cgagctgggc ctgcagggtc
     1021 tggaacaagc tgtcggcctt caggttcgga taattttccg acaccgccat caggcgcgac
     1081 agcgcgccgg acagctcgcc ctgcgcctgc tggaacgcct tcatcttcgc ttcgtcgttg
     1141 aggtcgtcgg tagtcagcgt gatcttgccg acctgcgcgc gcgcattggt tactgcttcg
     1201 agcgttgact gttcgtgctc ggcgtatccc ttcaccgtgt tgaccaggtt cggcacgagg
     1261 tcggcgcggc gctggtactg gttgatgact tcggaccagg cagccttgac cgcctcgtcc
     1321 ttggattgga tcgcgttgta gccgcagccg gccagcatga atgcggcgag cgcgagaatc
     1381 acgagtcgtt gcaggcgcat gggcatgggc agtcctccgg ttcgagtggt ggtcgagatg
     1441 gg
//
LOCUS       DASRVO010000053         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_105315, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000053 DASRVO010000000
VERSION     DASRVO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105315"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1763
                     /locus_tag="VFF05_00640"
     CDS             <1..1763
                     /locus_tag="VFF05_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZW50341.1"
                     /translation="LPVWIANFVLMGYGTGALMAVPAHDERDWEFATKYDLFIKMVIV
                     SDPVREAIRELGRDAASNTDAMSAALGESRAIDVVEPPAALALVRDFERAIIEDAAFT
                     DYGTLVNSGEFDGLDYRGAFAAIAARIEKEGKGARKVNFRLRDWGVSRQRYWGCPIPI
                     VYCPKCDAVPVPEDQLPVVLPENVHFMGVQSPIKADPEWRKCKCPQCGAAAERETDTF
                     DTFMESSWYYARYTSPGAADMVDARADYWLPVDQYIGGIEHAILHLLYFRFFHKLLRD
                     EGLVHSNEPATNLLCQGMVIAETFYREAANGNREWFNPADVDIERDERARVVGARLRF
                     DDQPVSIGGIEKMSKSKNNGVDPRTMVEKYGADTVRLFSMFAAPPEKSLEWNEAGVEG
                     MARFLRRLWRDAQAHIAQPDHPRVDAATLNAAQKNLRRQIHETIAKVGDDFGRRLSFN
                     TAIASAMELLNAVGKFDDMSDQGRAVRHEAFETLVLVLNPITPHICHALWLALGHRES
                     LLDVAWPQADPGALVKAEVTLAVQVSGKLRGTIEVAVDAPRDVVENAALAHADVAKFV
                     GDAVPKKIVIVPGKIVNIVI"
     gene            1777..>2219
                     /gene="lptE"
                     /locus_tag="VFF05_00645"
     CDS             1777..>2219
                     /gene="lptE"
                     /locus_tag="VFF05_00645"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZW50342.1"
                     /translation="MKMLRIAALVAVVLMLCACGFQLRGETNLPADLQRVHVAVSDAF
                     SPLKRDVEAALTRSGATVASASGEHIAEITLSAVSLAPVVRSVGANATVNEFSMVYHV
                     ELGIQGSDGKTLLTPQVIEHSRDYTFDQTQAIGNNAEQDEIKKEM"
BASE COUNT          423 a          677 c          725 g          394 t
ORIGIN      
        1 agctgccggt gtggatcgcc aatttcgtgc tgatgggtta cggcaccggt gcgttgatgg
       61 cggtgccggc acacgacgag cgcgactggg agttcgcgac gaaatacgat ctgttcatca
      121 agatggtgat cgtcagcgac ccggtgcgcg aagcgatccg cgaactcggc cgcgacgccg
      181 cgagcaatac cgacgcgatg tccgccgcgc ttggcgagag tcgcgctatc gacgtggtcg
      241 agccgcccgc cgcgctggcg ctggtgcgtg acttcgagcg cgccatcatc gaggacgcgg
      301 cgttcaccga ctacggcacg ctggtcaatt ccggcgagtt cgacggtctg gattatcgcg
      361 gcgcgttcgc cgccatcgcg gcgcgcatcg agaaggaagg caagggcgcg cgcaaggtga
      421 attttcgcct gcgcgactgg ggcgtatcgc ggcagcgcta ttgggggtgc ccgattccga
      481 tcgtctactg cccgaagtgc gacgccgtgc cggtgcccga ggaccaactt ccggtcgtgc
      541 tgcccgagaa cgtgcacttc atgggcgtgc aatcgccgat caaggccgat ccggaatggc
      601 gaaagtgcaa atgcccgcaa tgcggcgctg cggccgagcg cgagaccgac accttcgaca
      661 cgttcatgga atcgagctgg tactacgcgc gctacaccag tcccggcgca gccgacatgg
      721 tcgatgcgcg cgccgactac tggctgccgg tagaccagta catcggcggc atcgaacacg
      781 cgatcctgca cctgctgtac ttccgtttct tccacaagct gttgcgcgac gaagggcttg
      841 tgcattccaa cgagccggcc accaatctgc tgtgccaggg catggtgatt gccgaaacgt
      901 tctatcgcga ggcggcgaac ggcaatcgcg aatggttcaa tcctgccgac gtggacatcg
      961 aacgcgacga gcgcgcgcgc gtcgtcggtg cgcgcctgcg ctttgacgac cagccggtgt
     1021 ccatcggcgg catcgagaag atgtccaagt ccaagaacaa cggcgtcgat ccgcgcacga
     1081 tggtcgagaa atacggtgcc gacacggtgc gcctgttctc gatgtttgcc gcgccgccgg
     1141 aaaaatcgct ggaatggaac gaggctggtg tcgaaggcat ggcgcggttc ctgcgccggt
     1201 tgtggcgcga cgcgcaggca cacatcgcgc aaccggatca tccgcgcgtt gacgcggcga
     1261 cattgaatgc cgcgcagaaa aacctgcgcc ggcaaatcca cgaaaccatc gcgaaagttg
     1321 gcgacgactt tggccggcgc ttgtccttca atacggcgat cgcgtccgcc atggaactgc
     1381 tcaatgccgt cggcaagttc gacgacatgt ccgatcaagg ccgcgccgtg cgccacgagg
     1441 cgttcgagac cctggtgctg gtgctcaatc cgatcacgcc gcacatctgc cacgccctgt
     1501 ggcttgcgct cggccatcgc gaaagcctgc tcgacgtggc gtggccgcag gccgatcccg
     1561 gtgccttggt caaggctgaa gtcacgctgg ccgtgcaggt cagcggcaaa ttgcgcggta
     1621 ccatcgaagt ggcggtcgac gcgccacgcg acgtggtgga gaatgccgcg ctggcgcatg
     1681 cggacgtggc caagttcgtc ggcgatgcag tgccgaagaa gatcgtcatc gtgccgggga
     1741 aaatcgtaaa catcgtcatt tgaacggaat ccagtcatga aaatgctgcg aatcgcggcg
     1801 ctcgtcgccg tcgtcctgat gctctgcgcc tgcggcttcc agttgcgcgg cgagacaaat
     1861 ttgccggccg acttgcagcg tgtgcacgtg gccgtgtccg atgcgttcag tccgctcaag
     1921 cgcgatgtcg aagcggcgtt gacgcgttcc ggtgcaacgg ttgcaagcgc atccggcgaa
     1981 cacatcgccg aaatcacctt gtcggcggtg tcgctggcgc cggtggtgcg ttcagtcggt
     2041 gcgaacgcca cggtcaacga attttcgatg gtctatcacg tcgaactggg catccagggt
     2101 tcagacggaa aaaccctgtt gacgccgcag gtgatcgagc attcgcgcga ttacaccttc
     2161 gaccagaccc aggccattgg caacaatgcc gaacaggacg agatcaagaa ggagatgga
//
LOCUS       DASRVO010000054         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_365074, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000054 DASRVO010000000
VERSION     DASRVO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365074"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1134)
                     /locus_tag="VFF05_00650"
     CDS             complement(97..1134)
                     /locus_tag="VFF05_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50343.1"
                     /translation="MKRCIRCHAALLPTLGLFLAVTAHASTYCVHNGAELGLALADVS
                     DGGIANAHDNTILLASGTFATAGGPFTFNSSSGFALMIEGGYDSSCNTQDMTPGLSQL
                     DGGGTSQVINFQTNGNIGVRHVTIQHAYKVGSAGGGGQIYLNNAAAVAVFADNQVIDN
                     TDTFGTGGLTVFGVGTVTIAANLFAGNSAPAASALSTSMAAGSIIYLVNNTIAGNTNT
                     SGGNMITALGGTNAGGYASNNISYGNTNGDDFYLYSFQTFEFHNNDYHALNGVPTDGS
                     AGNIDLDPKFVGAGDYRLSGASPVLAGGLTNPVGGLPAVDLDGRAFPSSGRADMGAFE
                     ETIFIDAFESP"
     gene            complement(1220..1534)
                     /locus_tag="VFF05_00655"
     CDS             complement(1220..1534)
                     /locus_tag="VFF05_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50344.1"
                     /translation="MAEYIQAWQCIGCGKIEAPQPCIGVCRDRKVLFIGKDEHEAALA
                     EIERLRERLQSAASRLQRFGLAAPRAGQWEAAYTALRDEARALIAELDAALEAGSGEG
                     AG"
     gene            1686..2987
                     /locus_tag="VFF05_00660"
     CDS             1686..2987
                     /locus_tag="VFF05_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HZW50345.1"
                     /translation="MKINTTAQPSLRDIPLHVENPAPPDRDLRAALADLPRLLAAAPH
                     RMMFFGGATALLVTMTWWACFLGAGYFGHAFPAAPVPGGWAHAVFTQYGMLAMFIFGF
                     LLTVFPRWMGQPALPKRAYIPVFVGIFGGYLLSHVGLLNLRPLLIAGLALMLAGWLLA
                     LRALGGVLWRNRGKDRHAISCFTALCAGFVGLACFLAFTLGAPWQCAIFAIKIGTFGL
                     LLPIFFTVAHRMIPFFSSNIVGPSYKPFRPAWSLAVLWPLLLAHIGLDLAHRYDLLFL
                     VDAPLALFFAYHWIGWQPWKCLRPGLLAALHIALLWLPTAFVLFAVQSLVFRWACDFT
                     LGRAPVHALTVGFFASMLVAMVTRVTHGHSGRPLQMGAVPWLTFGLLQLVVLARLWAE
                     LANDSALWLVIAAFGWVLALAPWVLRSLWIYLTPRRDGMPG"
BASE COUNT          476 a         1039 c          972 g          634 t
ORIGIN      
        1 gaactcgaac tcgtcgataa accggcgctg cagaaactgg cgcggcatat cttgcgcgat
       61 tgacggcgcg cattgccgtc gctcgaaacc cgggatttac ggactctcga aagcgtcgat
      121 gaagatcgtt tcttcgaatg cgcccatgtc cgcacggccg ctgctcggga atgcacggcc
      181 gtccaaatcc acggcaggca gtccgccaac gggattcgtc aaacccccgg ccagtaccgg
      241 cgatgcgccg gagaggcgat agtcgccggc gccgacgaat ttcggatcca ggtcgatatt
      301 gccagcgctg ccgtccgttg gcacgccatt gagcgcgtgg taatcgttgt tgtggaattc
      361 gaaggtctgg aacgaataca ggtagaaatc gtcgccgttg gtgttgccgt aactgatgtt
      421 gttgctggca taaccgccgg cgtttgtgcc gccgagcgcg gtaatcatgt tgccgccgct
      481 cgtgttggta ttgcctgcga tcgtattgtt gaccaggtaa atgatcgaac ccgcagccat
      541 gctcgtcgac aacgcgctgg ccgccggcgc actgttgccc gcgaacaggt tggcggcgat
      601 ggtcaccgtg ccgacgccaa acacggtcag gccacccgtc ccaaacgtgt cggtgttgtc
      661 gatcacctgg ttgtcggcga acaccgcgac ggccgccgcg ttgttcaggt agatctgtcc
      721 gccgccgccg gccgacccaa ccttgtaggc gtgctggatc gtgacatggc gcacgccgat
      781 gttgccgttg gtctggaagt tgatgacctg gctggtgccg ccgccatcga gttggctcaa
      841 gccgggcgtc atgtcctgcg tgttgcagga actgtcgtag ccgccctcga tcatcagcgc
      901 aaagccgctg ctcgagttga acgtgaacgg gccgccggcg gtggcgaagg taccgctggc
      961 gagcaagatc gtgttgtcgt gcgcgttggc aatgccgccg tcgctgacat cggccagggc
     1021 caaccccagc tccgcgccgt tgtgcacgca gtaggtgctc gcatgggcgg tgacagccag
     1081 gaacaggccg agcgtcggca acaaagcggc gtgacaccga atgcaacgtt tcatgaagtc
     1141 tccagtccgg aaaggccaca caggtaaacg tcggcgacac cgcaaaccgt acatgggctc
     1201 gtggacgcac gggtccgcat tacccggctc cctcgccgga gcccgcttcc aacgccgcat
     1261 ccaattccgc gatcagcgcc cgcgcttcat cgcgcaaagc ggtgtaggcc gcttcccatt
     1321 gccccgcacg cggcgcggcg aggccgaagc gctgcaggcg cgatgccgcg gattgcagtc
     1381 gctcgcgcag gcgctcgatt tcggcgagcg cggcttcgtg ctcgtccttg ccgatgaaca
     1441 gcaccttgcg gtcgcggcac acgccgatgc acggctgcgg cgcttcgatc ttgccgcaac
     1501 cgatgcactg ccatgcctgg atgtattcgg ccatcgccgc ttgctccgga atccaaatcg
     1561 attgcggacg catcctagcg atgccccgat cgaccggcct tgatccacga caagccgcac
     1621 atgccgtcgc gcgtacaatg acgcaggtca agacaaactg tgccgcggcg cgcgacaatt
     1681 tgcgaatgaa aatcaatacg actgcccaac cctcgctgcg cgacatcccg ttgcatgtgg
     1741 aaaatcccgc cccacccgac cgcgacctgc gtgcggcgct cgccgacctg ccgcgcctgc
     1801 tcgccgccgc gccgcatcgt atgatgtttt tcggcggcgc gaccgcgttg ctcgtgacga
     1861 tgacgtggtg ggcgtgcttc ctgggcgccg gttatttcgg ccacgccttt cccgccgcgc
     1921 cggttcccgg cggatgggcg catgcggtgt tcacccagta cggcatgctg gcgatgttca
     1981 tcttcggttt cctgctcacc gtgttcccgc gctggatggg acagccagcg ttgcccaagc
     2041 gcgcctacat tccggtcttt gtcggtatct tcggcggcta tctgctctcg cacgtcggcc
     2101 tgctgaacct gcgtccgctc ctgatcgctg gactcgccct gatgctggcc ggctggctcc
     2161 tggccttgcg cgcgcttggc ggcgtgttgt ggcgcaatcg cggcaaggac aggcacgcca
     2221 tatcgtgttt caccgccttg tgcgcgggat tcgttggctt ggcctgcttc ctcgccttca
     2281 ccctcggcgc accgtggcag tgcgcgatct tcgccatcaa gatcggcacg ttcggattgc
     2341 tgctgccgat cttctttacc gtcgcgcacc gcatgattcc cttcttcagc agcaatatcg
     2401 tcgggccgag ttacaaacct tttcgaccgg cctggagcct ggccgtgctg tggccactgc
     2461 tgctcgcgca catcggtctc gacctggccc accgctacga cctgctcttt ctcgtcgacg
     2521 caccgctggc gctgttcttc gcctaccact ggatcggctg gcagccgtgg aaatgcctgc
     2581 gcccgggatt gctcgccgcg ctgcacatcg cgctgctgtg gctgccgacg gcgttcgtgt
     2641 tgttcgccgt gcaaagcctg gtcttccgct gggcctgcga cttcacgctc ggccgcgcgc
     2701 cagtgcatgc gctcaccgtc ggcttcttcg cctcgatgct ggtggcgatg gtcacgcgcg
     2761 tcacccacgg ccattccggg cgcccgctgc agatgggcgc ggttccctgg ctcacgttcg
     2821 gcctgctgca acttgtcgtg cttgcgcgcc tgtgggcgga actggccaac gacagtgcac
     2881 tgtggctggt gatcgccgcg ttcggctggg tgctcgcgtt agcgccgtgg gtgctgcgct
     2941 cgctgtggat ctacctgacg ccacggcggg acgggatgcc cggataaaaa cctgttgcgc
     3001 tcgacctctc gcgcgccggc agtgctcgaa atcctcattt acatctgagt aaactccggt
     3061 tccggcgcgc cggcggcgcg cgacctgccg tcgctcgcga cggtttttca gagatcccgt
     3121 g
//
LOCUS       DASRVO010000055         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_339340, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000055 DASRVO010000000
VERSION     DASRVO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339340"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="sufC"
                     /locus_tag="VFF05_00665"
     CDS             complement(<1..761)
                     /gene="sufC"
                     /locus_tag="VFF05_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZW50346.1"
                     /translation="MLEIDNLHARLAGDGREILKGIDLSVAAGEVHAIMGPNGSGKST
                     LAAVLAGRDGYEVTQGSVRYQGRDLLAMPADERAREGLFLAFQYPVEIPGVNNTYFLR
                     AAVNAGRRHRGEPELDAGQFLRLVRAQLKLLDIGDDLLQRPVNVGFSGGEKKRNEIFQ
                     MAMLEPKLAILDETDSGLDIDALRIVAKGVNDLRTPERAIVMITHYQRLLQYVEPDHV
                     HVLQDGRIVQSGDKSLAVELERTGYGAFEEAAGGAA"
BASE COUNT          210 a          383 c          342 g          209 t
ORIGIN      
        1 gccgcgccgc cggccgcttc ttcgaaggcg ccgtagccgg tgcgctccag ttcgacggca
       61 aggctcttgt cgccggactg cacgatgcga ccatcctgta acacgtgcac atggtccggc
      121 tcgacgtatt gcaggaggcg ctgatagtgc gtgatcatga cgatcgcgcg ctcgggtgtg
      181 cgcaggtcgt tgacgccctt ggcgacaatt cgcagcgcgt cgatgtcgag gccggagtcg
      241 gtttcgtcaa ggatcgccag cttcggctcc agcatcgcca tctgaaagat ttcgttgcgc
      301 tttttctcgc cgccggaaaa cccgacattg accggccgct gcagcagatc gtcgccgatg
      361 tcgagcagtt tcaattgggc acgcacgagg cgcaggaact ggccggcgtc gagctcgggc
      421 tcgccgcggt ggcgccgtcc ggcattgacc gcggcgcgca ggaaataggt gttgttgacg
      481 cccgggattt cgaccgggta ctggaacgcg aggaagaggc cctcgcgcgc ccgctcgtcg
      541 gccggcatcg ccagcaggtc gcgcccctga tagcgcacgc tgccctgcgt cacctcataa
      601 ccgtcgcgcc ccgccagcac cgcggccagc gtcgattttc ccgagccgtt cggccccatg
      661 atcgcatgca cctcgccggc cgcgaccgag agatcgatcc ccttcagaat ctcgcggcca
      721 tcgcccgcca gccgcgcatg caggttgtcg atttcaagca tcacaggacg taatcgcagc
      781 tatcggcttt cgacagatca gcgcgaaaca cctggcaccg ggagcgatcc gaatcctgcc
      841 gaacgaggct cgacgacgcc acgagtgaca gattagcgat cgctgccagc agcagtaaat
      901 aacgtgctat tttccgtgca cccggcgtgg gtcgacgcaa tactggaaat tcggtaatcg
      961 caaccattgc gcctactcca gccctagtgc ctttgggggc ggcaagattc atcacacctg
     1021 cacgcccatc cgttcggcga tgcgcgcgag caagaagata atccgctcta cttgttcttc
     1081 gaggacagcg aaccggcgct ccatcgccgc aaaccgcttt tcgagcctgc aaaagcgctc
     1141 ctcc
//
LOCUS       DASRVO010000056         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_27555, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000056 DASRVO010000000
VERSION     DASRVO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27555"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFF05_00670"
     CDS             complement(<1..436)
                     /locus_tag="VFF05_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HZW50347.1"
                     /translation="MNPPRIEALGDRALLIHCGEIIDAAINAQALAIARALEDARLPG
                     ILDIAPAYASVCVRYEPAAWASTDPAQSPFDRVAERLRTLVDNAASDAATDTGSIEIA
                     VTYGGPCGPDLADVAAYTRLSEEEVIARHVAAEYRVAMLGFTP"
     gene            complement(433..861)
                     /locus_tag="VFF05_00675"
     CDS             complement(433..861)
                     /locus_tag="VFF05_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZW50348.1"
                     /translation="MLAEPDFILDPRLAADTHLVGDLDLSRVLLMDDARFPWLILVPR
                     RANLRELIDLDIAAQQLLLAEVDRCARILRTLEKPDKLNIAALGNVVAQLHVHAIARR
                     ANDAAWPRPVWGFGEREPYASDALSTRLVAIRRALEMPGA"
     gene            complement(864..1532)
                     /locus_tag="VFF05_00680"
     CDS             complement(864..1532)
                     /locus_tag="VFF05_00680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZW50349.1"
                     /translation="MGERINLNTAGMHCIGAYLAKPAGKPKGGVVVVQEIFGVNAHIR
                     SVADGYAVHGYTAIAPAFFDFVENDVELAYDKPGIDKGKALAMEVGLERAIEAVASAA
                     ESIRSSGKVGVVGYCWGGTVALRAAQVLGLPASSYYGGRNVGFLDQPLKAPVIFHFGA
                     DDPSIPPEAIAKQRAAWPDAPVYVYEHCGHAFNRDADPHAYNAAAAKLALERSLALFA
                     RSLA"
BASE COUNT          280 a          540 c          605 g          292 t
ORIGIN      
        1 cgggcgtgaa gccgagcatg gcgacgcggt attccgcggc gacgtggcgc gcgatcactt
       61 cttcttcgga caatcgagta tacgccgcca catcggcgag gtcgggtccg catgggccgc
      121 cgtaggtcac cgcaatctcg atgctgcccg tgtccgtcgc cgcatcggat gcggcattgt
      181 ccacaagtgt acgcagacgc tcggcgacgc gatcgaaggg cgattgtgcc gggtcggtgc
      241 tggcccacgc ggctggctcg tagcgcacgc acacgctggc gtaggcgggc gcgatgtcca
      301 agatgccggg aagccgggca tcttcgagcg cgcgcgcaat cgcgagcgcc tgcgcgttga
      361 tcgcggcgtc gatgatttcg ccgcagtgga tgagcaacgc gcgatcgccc aacgcttcga
      421 tgcgtggcgg attcatgcgc cgggcatctc cagcgcgcgg cgtatcgcca cgaggcgcgt
      481 tgacagcgca tcggatgcgt aaggctcgcg ttcgccgaat ccccacaccg gccgtggcca
      541 ggccgcatcg ttcgcgcggc gcgcaatcgc atgcacgtgc agttgcgcga ccacattgcc
      601 gagcgcggcg atgttgagct tgtcgggttt ttcaagcgtg cgcaggatgc gagcgcagcg
      661 gtcgacctcg gcgagcagca gttgctgagc ggcgatgtcg agatcgatga gctcgcgcag
      721 attcgctcgc cgcggcacga ggatcagcca cggaaaacgc gcatcgtcca tcagcagcac
      781 gcgcgacagg tcaagatcgc cgacgagatg cgtgtcggcg gccaggcgcg gatcgaggat
      841 gaaatccggt tctgccagca tggtcaggcg agactgcgcg cgaacagcgc cagcgagcgt
      901 tcgagtgcga gtttcgccgc cgccgcgttg taggcgtgcg gatcggcgtc gcggttgaag
      961 gcgtggccgc aatgctcgta cacatacacc ggcgcatccg gccaggcggc gcgttgcttc
     1021 gcgatggcct cgggcggaat ggaggggtcg tccgcgccga agtggaaaat caccggcgct
     1081 ttgagcggct ggtcgaggaa tccgacgttg cgtccgccgt agtagctcga cgccggcagg
     1141 cccagcacct gcgccgcgcg cagcgccacg gttccgcccc agcagtatcc gaccacgccg
     1201 accttgccgc tggaccggat cgattccgcc gcgctggcga cggcttcgat cgcgcgttcc
     1261 aggccgactt ccatcgcgag tgccttgccc ttgtcgatgc cgggtttgtc gtaggcgagt
     1321 tccacgtcgt tttcgacgaa atcgaagaac gccggtgcga tggcggtgta gccgtgcacg
     1381 gcgtagccgt cggcaacgct gcggatatgc gcgttgacgc cgaagatttc ctgcaccacc
     1441 acgacgccgc ctttcggttt gccggcgggc ttggccaggt aagcgccgat gcagtgcatg
     1501 ccggcggtgt tgaggttgat gcgttcgccc atgtcgcgct ccttgcagga atcggactca
     1561 gcctaacccg cgccggcggc ttgtcaactc ggacaatgtt ggacgctacg gaaaccgtcg
     1621 cgagcgaaga caggtcgcgt gccggcagca cgcaggggcc ggggcttatc tggatgcaaa
     1681 tgaggttccg aggactgccg gcgcgcggga tgtcgag
//
LOCUS       DASRVO010000057         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_1409, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000057 DASRVO010000000
VERSION     DASRVO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /gene="sdhC"
                     /locus_tag="VFF05_00685"
     CDS             <1..375
                     /gene="sdhC"
                     /locus_tag="VFF05_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZW50350.1"
                     /translation="RVNMWQSVLHRLTGLFLCLGALLLAWGLIAAATGAEAWNAFAGF
                     CASGIGFALLILWTWALLFHLCNGIQHLFRDTGMGYDAAQRDHSRTPSYFATGWIVIV
                     ASVALTALVWICLFVRASGGAA"
     gene            372..767
                     /gene="sdhD"
                     /locus_tag="VFF05_00690"
     CDS             372..767
                     /gene="sdhD"
                     /locus_tag="VFF05_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZW50351.1"
                     /translation="MSAIGENTLRTPLKNVRGLGSALSGVRHWWLQRLTAVALLPLSL
                     WFLWLCAGLAHADYVTVRAAIAQPVHSVLLVALVVSLFWHGALGLQVVIEDYIHTRWQ
                     EVALQIAVRFGAFLAAVACIFAIARIAIG"
     gene            771..>2455
                     /gene="sdhA"
                     /locus_tag="VFF05_00695"
     CDS             771..>2455
                     /gene="sdhA"
                     /locus_tag="VFF05_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZW50352.1"
                     /translation="MESYKIHEHKYDVVVVGAGGSGLRATFGMAEQGLNTACITKVFP
                     TRSHTVAAQGGIAAALGNMGEDDWRFHFYDTVKGGDWLGDQDAIEYMCKNAPAAVIEL
                     EHYGVPFSRTEAGKIYQRPFGGMTTNYGKGTAQRTCAAADRTGHAMLHTLYQQSLKHD
                     ARFYIEYFALDLIFDSDGACRGVLALDMNEGELHLFRAHAVVLATGGYGRAYFSATSA
                     HTCTGDGGGMALRAGLALQDMEFVQFHPTGIYGAGCLITEGVRGEGGYLTNSKGERFM
                     ERYAPNAKDLASRDVVSRAITMEIREGRGVGEHKDHAYLNLMHLGPEVIHERLPGIAE
                     SARIFAGVDVTKQPIPVLPTVHYNMGGIPTNYHGEVVQKRGNDADAVVPGLFAVGEAA
                     CVSVHGGNRLGSNSLLDLVIFGRAAAHRAGETIKPGTPHKNLPASSCDAALAHFDKAR
                     NAKGATPTADLRLKMQRVMQQDAAVFRTGDTLKQGCQNISAAFDAFADVKVTDRSLVW
                     NSDLIETLELHNLLGQAVATMYSAEQRHESRGAHAREDFPDRDDKNWMKHTLVG"
BASE COUNT          424 a          784 c          802 g          445 t
ORIGIN      
        1 cgtgtaaaca tgtggcagtc ggtgttgcac cgcctgaccg gactgttcct gtgtctgggc
       61 gcgctcttgc tggcctgggg gctgatcgcc gctgcgactg gcgccgaggc ctggaatgcg
      121 tttgccggat tctgcgcaag cggaatcggc ttcgccctgc tgatcctgtg gacatgggcg
      181 ctgctgttcc acctgtgcaa cggcatccag cacctgtttc gcgacaccgg catgggttac
      241 gacgcggcgc agcgcgatca ttcgcgcacg ccgagctatt tcgctaccgg ctggatcgtg
      301 attgtggcga gcgtcgcgtt gacggctctg gtctggattt gcctgttcgt tcgcgccagt
      361 ggaggtgcgg catgagcgcg atcggcgaaa ataccctgcg cacgccgctg aagaacgtgc
      421 gtggactcgg ctcggcactg tccggcgtgc ggcattggtg gctgcaacgc ctgaccgcgg
      481 tggcgctgct gccgctgagt ttgtggttcc tgtggctgtg tgccggtctg gcacacgccg
      541 attacgtcac ggtgcgcgcg gcgatagccc agccggtgca ttcggtgctg ctggttgcgc
      601 tcgtggtcag cctgttctgg cacggcgcac tcggcctgca agtcgtcatc gaggactaca
      661 tccacacgcg ctggcaggaa gtcgcgttgc agatcgcggt gcgcttcggc gccttcctcg
      721 ccgccgtcgc ctgcatcttc gccattgccc gcatagccat cggataagcc atggaaagct
      781 acaaaattca cgaacacaaa tacgatgtcg tcgtggtcgg cgcgggcggt tccggcctgc
      841 gtgccacctt cggcatggcc gagcaagggt taaacacggc gtgcatcacc aaggtgttcc
      901 caacgcgttc gcatacggtc gccgcgcaag gtggcatcgc tgccgcactc ggcaacatgg
      961 gcgaggacga ctggcgattc catttctacg acacggtgaa gggcggcgac tggctcggcg
     1021 accaggatgc gatcgagtac atgtgcaaga acgcgccggc cgcggtgatc gagctcgaac
     1081 actacggcgt gccgttctcg cgcaccgaag ccggcaagat ctaccagcgt ccgttcggcg
     1141 gcatgaccac gaactacggc aagggtacgg cgcagcgcac ctgcgcggca gccgaccgta
     1201 ccggtcacgc gatgctgcat acgctgtacc agcaatcgct caagcacgac gcgcgtttct
     1261 acatcgaata cttcgcgctg gacctcatct tcgacagtga cggcgcctgc cgcggcgtgc
     1321 tcgcgctgga catgaacgaa ggcgagttgc acttgttccg cgcgcacgcc gtggtactcg
     1381 ccaccggcgg ctatggacgg gcgtatttct ccgcgacgag cgcgcacacc tgcaccggcg
     1441 acggcggtgg catggcgctg cgcgcgggtc ttgcgctgca ggacatggaa ttcgtgcaat
     1501 tccacccaac cggcatctac ggggccggct gcttgatcac cgaaggcgtg cgcggcgagg
     1561 gcggctacct gaccaattcc aagggcgagc gcttcatgga gcgctatgcg ccgaacgcca
     1621 aggacctggc ctcgcgcgac gtggtcagcc gtgcgatcac gatggaaatc cgcgaaggcc
     1681 gcggcgttgg cgagcacaag gatcacgcgt atctgaacct gatgcacctc ggccccgagg
     1741 tcatccacga acgcctgccc ggcatcgcag aaagcgcgcg catcttcgcc ggcgtggacg
     1801 tgaccaagca gccgatcccg gtgttgccga cggtgcacta caacatgggt ggcatcccga
     1861 cgaattacca tggtgaagtc gtgcagaagc gcggcaacga cgcggatgcc gtggtaccgg
     1921 gtctgtttgc ggtcggcgag gcggcctgtg tgtccgtgca cggtggcaat cgcctcggct
     1981 ccaattcctt gctcgatctc gtcatcttcg gtcgcgccgc tgcgcatcgt gctggcgaga
     2041 cgatcaagcc cggtaccccg cacaagaacc tgcccgcatc gtcctgcgac gcggcactcg
     2101 cgcacttcga caaggcgcgc aatgcgaaag gcgcgacacc gaccgccgac ctgcgcctga
     2161 agatgcagcg cgtgatgcag caggacgctg ccgtgttccg caccggcgat acgctcaagc
     2221 aaggttgcca gaacatttcc gccgcgttcg atgcgttcgc cgatgtcaag gtgaccgatc
     2281 gctcgctggt ctggaattcc gacctcatcg aaacgctgga actgcacaac ctgctcgggc
     2341 aggccgtggc gacgatgtat tcggccgagc agcgccacga gagtcgcggc gcgcacgcgc
     2401 gcgaggattt cccggatcgc gacgacaaga actggatgaa gcacaccttg gtcgg
//
LOCUS       DASRVO010000058         1473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_79566, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000058 DASRVO010000000
VERSION     DASRVO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79566"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..419)
                     /locus_tag="VFF05_00700"
     CDS             complement(243..419)
                     /locus_tag="VFF05_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50353.1"
                     /translation="MAKNEDTRDASLRVSKKTRDSLKAAAAVAGRPLYDVTNEAINNY
                     LASVKSGGARKGKK"
     gene            complement(575..1363)
                     /gene="tatC"
                     /locus_tag="VFF05_00705"
     CDS             complement(575..1363)
                     /gene="tatC"
                     /locus_tag="VFF05_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZW50354.1"
                     /translation="MGRAESTPSGDEDGGGNEQSFISHLIELRARLLKAIAAVLVVLV
                     ALLPFADRMYHFIAEPLLQRLPQGSHMIAIEVASPFMTPMKLAAVVALFLAMPAVLYQ
                     LWRFVAPGLYRHEKKLAIPLLAASVGMFYLGCAFAYYLVLPKVFVFLLGVTPDGVAIM
                     TDIGKYLDFVLTMFLAFGVCFEVPVVVFLLALLGVVNAEQLTRQRPYVVLGVFVIAAV
                     LAPPDALSMLMLAIPMCLLYELGVVAVRLFVRRARAGAQASTKT"
BASE COUNT          300 a          447 c          476 g          250 t
ORIGIN      
        1 cgtgcctaca tccgcgcccg cgacggcgcg ctggcggacg aggatttcga cgtaccggga
       61 atgctgcgcc aggtccgcgc cacgccgcag gcacgtggcg tgttgcggcg gttcgggcgt
      121 ctcctcagga attgagtcgc ctgaaatcgc atcgtccaaa ttgaatcggc ggaaaacaaa
      181 aaacggcccg ctcgggccgt ttttgctggt gcatccgcgc acgctgcgcg gacggagaat
      241 tcttacttct tgcctttgcg cgcaccgccc gacttgaccg aggcgagata gttgttgatc
      301 gcctcgttgg tgacgtcgta caacggacga ccggcgacgg ccgcggcggc cttcaaactg
      361 tcgcgcgtct tcttcgacac gcgcaggctg gcgtcgcgtg tatcttcatt ctttgccatg
      421 ttcatctgct cctgttttcg gggtgtgcgc aacggatgtg cgcgaagtgg aaaatgtgtc
      481 aaatgcggca ttgcagtcta tgccagaata ccgcaaaaga caacgcagca ccggcgcaaa
      541 cgacgctgca aagcggcatc gacggggagg acgatcaggt cttcgttgaa gcttgcgcgc
      601 cggcccgtgc gcggcgcacg aacaggcgca cggcgaccac gccgagctca taaagcaggc
      661 acatcgggat ggcgagcatg agcatcgaca gcgcatccgg cggcgccagc acggccgcga
      721 tcacgaacac gccgagcacc acatacgggc gttgccgggt cagttgttcg gcattgacga
      781 cgccgagcaa ggcgagcagg aacaccacga ccgggacctc gaaacacacg ccgaaggcga
      841 ggaacatggt cagcacgaaa tccaggtact tgccgatgtc ggtcatgatc gccacgccgt
      901 ccggcgtgac gccgagcagg aacacgaata ccttgggcag caccaggtaa tacgcaaacg
      961 cacagccgag gtaaaacatg ccgacgctgg cagcgagcag tgggatggcg agtttcttct
     1021 cgtgccggta aagcccgggc gccacgaaac gccacaattg atacaacacg gccggcattg
     1081 ccaggaacag cgcgaccacc gctgcgagtt tcatcggcgt catgaacggc gacgccactt
     1141 cgatcgcgat catgtgcgaa ccctgcggca ggcgctgcag cagcggttcg gcgatgaagt
     1201 ggtacatgcg atcggcgaat ggcagcaacg caaccagcac cacgagcacg gcggcgatcg
     1261 ccttgagcag gcgtgcgcgc agttcgatca ggtgagagat gaaggattgc tcgttgccgc
     1321 cgccatcctc gtccccactt ggtgtggatt cagcgcggcc catcggatgt tgccggtgga
     1381 tcgggtggcg cattcgattt tggcgggccg tgtacggctt cgcgcaacgc ggcgctgatg
     1441 tcggcacgcg gatcgagttg atccgcctcg cgt
//
LOCUS       DASRVO010000059         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_339456, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000059 DASRVO010000000
VERSION     DASRVO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339456"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1045)
                     /gene="fabD"
                     /locus_tag="VFF05_00710"
     CDS             complement(92..1045)
                     /gene="fabD"
                     /locus_tag="VFF05_00710"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZW50355.1"
                     /translation="MQAANLAFVFPGQGSQSLGMLAELGSEYSAVKSAFDEASTGAGV
                     DLWVLSQRGPEADLNRTENTQPALLAAGVAVWRVWHERGGPTPALLAGHSLGEYAALV
                     CAGVLGLREAAALVAGRGRLMQAAVPPGVGAMAAILGGDDAQIAQVCAEVAEGEVVAP
                     VNYNAPGQLVIAGNAAAVDRAIARLAELGVRKAVKLAVSVPSHCALMRGAAGELARKF
                     DAITWSLPKIPVIQNVEAKAYDNIEDIRDALARQLYSPVRWTESVQQLAALGATRVAE
                     CGPGKVLTGLLKRIERALDGRGIGAPGDMTQALGEWSGESR"
BASE COUNT          209 a          398 c          353 g          184 t
ORIGIN      
        1 atacggtaac tcgcatcatc cctgatgctc gcccttcggg ccggctccgc cgttcgggcg
       61 cgctccatgc gtgcctcgtc aacgcatcgc gtcagcgcga ctcgcccgac cactctccaa
      121 gagcttgggt catgtcaccc ggcgccccga tgccacggcc gtcgagcgcg cgctcgatgc
      181 gcttgagcaa tcccgtcagc accttgccgg gaccgcattc ggcgacgcgg gtggcgccaa
      241 gcgcggcaag ctgctggacg ctttcggtcc agcgcaccgg tgaataaagc tggcgcgcaa
      301 gggcgtcgcg aatatcctcg atattgtcgt aggctttcgc ttcaacattc tgaattacag
      361 gaatttttgg aagcgaccat gtgatggcgt cgaacttgcg cgccagttcg cctgcggcac
      421 cgcgcatcag cgcgcaatgc gatggcacgc tgaccgccag cttcaccgcc ttgcgcacgc
      481 cgagctcggc caggcgcgca atcgcgcgat ccactgcggc ggcattgccg gcgatgacga
      541 gttgtccggg tgcgttgtaa ttgaccggcg cgaccacttc gccttcggcg acttcagcgc
      601 aaacctgcgc gatctgcgcg tcgtctccgc caaggatcgc ggccatggca ccgacgcccg
      661 gcggcaccgc agcctgcatc aggcgtccgc gtccggccac cagcgcggca gcctcgcgca
      721 ggccgagcac accggcgcaa accagcgcgg catattcgcc caaactgtgt ccggccagca
      781 acgcgggcgt cggaccaccg cgctcatgcc atacgcgcca tacggccacg ccggccgcga
      841 gcagcgccgg ctgcgtgttc tcggtgcgat tgagatccgc ttcgggaccg cgctgcgaaa
      901 gcacccacag atcgacgcct gcgcctgtgg aagcctcgtc gaaagcggat ttgaccgcgg
      961 aatattcgct gcccagttcg gcgagcatgc cgagcgactg cgaaccctgg ccgggaaaga
     1021 caaaagcgag attggctgcc tgcatgagcg tcagcgattt ctacaagaag gggaaggtcg
     1081 atcaataccg gagcaacgcc gagccccagg tgaatccgcc gccgaaggct tccagcagca
     1141 acaa
//
LOCUS       DASRVO010000060         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_183551, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000060 DASRVO010000000
VERSION     DASRVO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183551"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VFF05_00715"
     CDS             complement(<1..298)
                     /locus_tag="VFF05_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZW50356.1"
                     /translation="MSVAVVGSFNVDHVWRVATLPQAGATLSGDYASGPGGKGFNQAM
                     AARRAGAITAFVCALGDDAGAQLARALCASDDIDLRARACDAPTGTAGIYVDAQG"
     gene            complement(295..654)
                     /locus_tag="VFF05_00720"
     CDS             complement(295..654)
                     /locus_tag="VFF05_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HZW50357.1"
                     /translation="MLLALRAREPLFHRPEFGTARVDFERMIVDDYWEIGASGRRYDR
                     AFVLDVLERRHAVQHDDPWETSDFRCRALGARTWLLTYTLQQGERLSRRMTLWREDAD
                     GWRALYHQGTLVQDAAP"
     gene            complement(677..1954)
                     /locus_tag="VFF05_00725"
     CDS             complement(677..1954)
                     /locus_tag="VFF05_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HZW50358.1"
                     /translation="MSLKLRLIVMNFLQFFVWGAWLITIGRYWFENQHWSGTSFGAIF
                     STMGISALFMPALMGVVADKWINAEKLYGVLHIGGAIMLAMVPTAGDPQTLFWVMLVN
                     MIFYMPTIALAITVAYNALKEENLDVVRDYPPIRVWGTIGFIAALWTTSLLGLETSPG
                     QFYVAAAASALLGLYAFTLPPCPPRMKRAESRSLLDVLGLTSFRLFKDRNMAVFFVFA
                     MLLGAALQLTNAYGGTFLGEFSTHPAFKDALAVKYPAIIMSISQVSETLFILTIPFFL
                     KRFGIKTVMTISMIAWFLRFGLFAYGDPGPGLWMIVLSCIVYGMAFDFFNISGSLFVE
                     GQADPKIRASAQGLFMLMTNGIGAVLGSSISGWMIDRWFTAASGAKDWHGIWVTFALY
                     SLVMAIAFVPLFKHKHDPHRVIAVHGAGDVDNP"
BASE COUNT          396 a          684 c          732 g          353 t
ORIGIN      
        1 ggccttgtgc atcgacgtag atgccggcgg tgccggtcgg cgcgtcgcag gcacgcgcgc
       61 gcagatcgat gtcgtcgctg gcgcacaacg cgcgcgcgag ttgtgcaccg gcgtcgtcgc
      121 cgagtgcaca cacgaatgcc gtgatcgcgc ccgcgcggcg cgcggccatc gcctgattga
      181 aacccttgcc gccgggaccg ctggcatagt cgccgctcag tgtcgcgccg gcctgcggca
      241 gcgtcgccac gcgccagaca tgatcgacgt tgaacgagcc gacgacggcg acgctcacgg
      301 cgctgcgtcc tgtaccagcg tgccctggtg gtaaagcgcg cgccagccgt cggcatcctc
      361 gcgccacagc gtcatgcgcc ggctcaggcg ttcgccctgt tgcagcgtgt acgtgagcag
      421 ccaggtgcgt gcgccgagcg cacggcaacg gaagtccgag gtttcccacg gatcgtcgtg
      481 ctgcacggcg tgacgccgtt cgagtacgtc gaggacgaag gcacggtcgt agcgtcgccc
      541 ggatgcgccg atttcccagt aatcgtccac gatcatgcgc tcgaaatcga cgcgcgcggt
      601 gccgaactcc gggcgatgga acagcggctc gcgcgcgcgg agcgcgagca gcactgcggc
      661 ttgggtttcc ccgccgtcag ggattgtcga catcgccggc accgtgcacg gcgatcacgc
      721 gatgcgggtc gtgcttgtgc ttgaacagtg gcacgaacgc gatggccatg acgagcgagt
      781 acagcgcaaa cgtgacccag atgccgtgcc agtccttcgc gccggacgca gcggtgaacc
      841 agcggtcgat catccagccg ctgatcgagc tgcccagcac ggcgccgatg ccgttcgtca
      901 tcagcatgaa caggccctgc gcgctggcgc ggatcttcgg atcggcctgg ccctcgacga
      961 acagcgagcc ggagatgttg aagaagtcga acgccatgcc gtagacgatg cacgagagca
     1021 cgatcatcca caggcccgga cccggatccc cataggcgaa caggccgaag cgcaggaacc
     1081 aggcgatcat gctgatcgtc atcaccgtct taatgccgaa gcgcttgagg aagaacggga
     1141 tggtcaggat gaacagggtt tccgatacct gcgagatgga catgatgatc gcgggatact
     1201 tcaccgcgag cgcgtccttg aacgccggat gggtgctgaa ttcgcccagg aaggtgccgc
     1261 cgtaggcatt ggtcagttgc agcgccgcgc cgagcagcat ggcgaagacg aagaacaccg
     1321 ccatgttgcg atccttgaac aggcggaacg aggtcagtcc aagcacgtcg agcagggagc
     1381 ggctttcggc gcgtttcatg cgcggtggac atggcggcag ggtgaaggcg tacaggccga
     1441 gcagggccga tgcggcggcg gcgacgtaga actgcccggg cgaggtttcc aacccgagca
     1501 ggcttgtcgt ccacagcgcg gcgatgaagc cgatcgtgcc ccacacgcgg atcggcggat
     1561 agtcgcgcac cacgtccagg ttttcttcct tcagcgcgtt gtaggccacc gtgatcgcca
     1621 gcgcgatcgt cggcatgtag aagatcatgt tgaccagcat cacccagaac agggtttgcg
     1681 ggtcgcccgc ggtcggcacc atggccagca tgatcgcgcc gccaatgtgc agtacgccgt
     1741 agagtttttc ggcgttgatc cacttgtccg cgaccacgcc catcagcgcc ggcatgaaca
     1801 gcgccgagat gcccatggtg gagaagatcg cgccgaagct ggtgcccgac cagtgctggt
     1861 tctcgaacca gtagcggcca atcgtgatca gccacgctcc ccacacgaag aattgcagga
     1921 agttcatcac gatcaggcgc agtttcaggc tcacgcggct ctcccccggc ggcaagatgg
     1981 atcgggcgag ggtagggcat ggcgcacatc cgggcaagcc gtgccctgct agacttggcg
     2041 gcggagaggg ggtacgcacg atgcgatttg cgctcggcat ccggcagcgc ctgctgctca
     2101 gccatttgtt cgccgtgatc gtggttgccg gcacgttcgg cgcggtcgtc tattgggtcg
     2161 ccgcc
//
LOCUS       DASRVO010000061         1167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_183573, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000061 DASRVO010000000
VERSION     DASRVO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183573"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VFF05_00730"
     CDS             complement(<1..530)
                     /locus_tag="VFF05_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50359.1"
                     /translation="MLLAAMVSAAPAQTLSPSLYQEMRWRMIGPFRGGRTVAIAGIPA
                     QPNVFFISSVNGGVWKTTDYGRTWNPIFDGQPTGSIGALAVAPSNPDVMYVGSGEGLQ
                     RPDLATGDGIYKSTDGGRTWTHLGLRDGQQIASILVDPRDPDRIFVAVLGHPYGPNPE
                     RGVFRSTDGGQSFQKIL"
     gene            complement(647..>1167)
                     /locus_tag="VFF05_00735"
     CDS             complement(647..>1167)
                     /locus_tag="VFF05_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZW50360.1"
                     /translation="LGTADAVLAVESFAAGGEFVVLNSDNLYPVECLRALRQLDGPGL
                     PVFRADSLVRGGNIPAERLLDFAVLSIAADGHLTDIVEKPQRPRSEPLPPATLISMNL
                     WRFGPEIFPFCRAVAPSPRQERELPHAVRDAVAAGMRLRTFVCSGGVLDLSRRADIAA
                     VAERLANIACNP"
BASE COUNT          226 a          333 c          434 g          174 t
ORIGIN      
        1 agaatcttct ggaacgattg gccgccgtcg gtggagcgaa agacgccgcg ctccgggttc
       61 gggccgtagg gatggccaag cacggcgaca aagatgcggt cgggatcgcg cgggtccacc
      121 aggatggagg cgatctgctg gccgtcgcgc aggccgaggt gcgtccaggt gcggccgccg
      181 tcggtggact tgtagatgcc gtcgccggtc gcgaggtcgg ggcgctgcag gccttcgccg
      241 ctgcccacgt acatgacgtc gggattcgag ggcgcgacgg cgagcgcgcc gatcgagcct
      301 gtcggctggc cgtcaaagat cggattccag gtgcggccgt agtcggtcgt cttccacact
      361 ccgccgttga ccgaggaaat gaaaaataca ttgggctgcg cgggaatgcc ggcgatggcg
      421 acggtgcggc cgccgcgaaa gggtccgatc atgcgccagc gcatctcctg gtagagcgat
      481 ggggagagag tttgcgccgg ggccgcggaa accatcgcgg ccagaagcac gcagcacacg
      541 ctgagacgat gcacgcggtc ctcctggaaa caagcgagga taacaaaaac ggtagttagg
      601 agttaggagt caggagttag tggttcgtgg ttaggagttt gcacgattaa gggttgcagg
      661 cgatgttcgc caggcgttct gcaaccgcgg caatgtccgc tcggcgcgaa aggtccagca
      721 cgccgccgga acagacgaac gtacgcaggc gcatgccggc ggccacggcg tcgcgcaccg
      781 cgtgcggcag ctctcgctcc tgccgtggag atggagcaac agcgcggcag aagggaaaga
      841 tttccgggcc aaagcgccac aggttcatgc tgatcaaggt ggcgggagga agcggctcgg
      901 agcgcggccg ctgcggcttt tccacgatgt cggtgagatg gccgtctgca gcgatgctga
      961 gaaccgcgaa gtcgagcagc cgttccgcgg gaatgttgcc gccgcgcacc agggaatcgg
     1021 cgcgaaagac gggcaggccg gggccgtcga gctgtcgcag ggcgcgcagg cattcaacgg
     1081 gatagagatt gtcggaattc aacaccacga actcgccgcc ggcggcgaaa gattccaccg
     1141 ccagcacggc gtcggcggtg ccgaggg
//
LOCUS       DASRVO010000062         1359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_313355, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000062 DASRVO010000000
VERSION     DASRVO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313355"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1359)
                     /locus_tag="VFF05_00740"
     CDS             complement(<1..>1359)
                     /locus_tag="VFF05_00740"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZW50361.1"
                     /translation="DAGYDKDRLEAAAPRDMELPFDSERKRMTTLHRRHDGFIAYTKG
                     APESVLPLCVAMLTASGTASLPRDEILAEAERMAAEGLRVLAIAQRSWPSLPQDAQAS
                     VVETELLFIGLVGLMDPPRREAPHAIELCRSAGITPVMITGDHPATARAIAERIGIDG
                     SAVMTGKELAQLNASEFQKRVQAIRIYARVDPAQKIRIVEALQARGEFVAMTGDGVND
                     APAIKRADIGIAMGREGTDVAREASSLVLLDDNFATIVSAVREGRRIYDNLRKFIKYA
                     MTCNSAEIWTIFLAPFLGLPIPLLPIHILWINLVTDGLPALALTAEPAERSIMRRPPR
                     PLQESIFAHGMWQHMLWVGLLMAGLTLFVQAWAYHTGHAHWQTMVFTVLTLTQLVHVL
                     AIRSEKESLFAIGLFSNRPMTLTILTTFLLQMATIYVPALNPIFKTEALDAGELALCL
                     VI"
BASE COUNT          239 a          406 c          455 g          259 t
ORIGIN      
        1 atatcaccag gcatagcgcc aactcgccgg catccagcgc ttcggtcttg aatatcgggt
       61 tgagcgccgg cacgtaaatg gtcgccatct gcagcagaaa agtggtcagg atggtcagtg
      121 tcatgggccg gttggaaaac agaccgatgg cgaacaatga ttccttttcc gagcgtatcg
      181 ccaacacatg gacgagttgg gtcaatgtca gcaccgtgaa caccatggtc tgccagtgtg
      241 catggccggt atgataggcc cacgcctgga cgaacagcgt gagtccagcc atcagcaggc
      301 ctacccagag catgtgctgc cacatgccgt gcgcgaagat gctctcctgc aatggacgcg
      361 gcgggcggcg catgatactg cgctcggcag gttcggcagt cagcgcgagg gcgggcaggc
      421 cgtcggtgac caggttgatc cacaggatgt ggatcggcag caacgggatc ggcaggccaa
      481 ggaacggcgc gagaaagatg gtccagattt ccgccgagtt gcaggtcatc gcgtacttga
      541 tgaatttgcg caggttgtcg tagatgcgcc ggccttcgcg cacggcgctg acgatggtgg
      601 cgaagttgtc gtcgagcagc accaggctgg atgcctcgcg tgccacgtcg gtgccttccc
      661 tgcccatggc gatgccgatg tcggcacgct tgatggcagg ggcgtcgttg acgccgtcgc
      721 cggtcatcgc gacgaattcg ccgcgcgcct gcagcgcctc gacgatgcgg attttctgcg
      781 cgggatcgac gcgcgcatag atgcggatgg cctgtacccg tttctggaat tccgaagcgt
      841 tcaattgcgc gagttctttg ccggtcatta ccgcgctgcc gtcgatgccg atgcgttcgg
      901 caatcgcgcg cgcggtggcg ggatggtcgc cggtaatcat cactggcgtg atgccggcgc
      961 tgcggcacag ttcgatcgca tggggcgctt cgcggcgcgg cgggtccatc agtccgacca
     1021 gcccgatgaa cagcaattcc gtctcgacga cggaggcttg cgcatcctgc ggcagagacg
     1081 gccagctgcg ctgggcgatg gccagcacgc gcagtccttc cgccgccatg cgttcggcct
     1141 cggcgaggat ttcgtcgcgc ggcagggatg ccgtgccgga tgcggtgagc atcgcaacac
     1201 acaacggcag cacgctttcc ggcgcaccct tggtgtaggc aatgaaccca tcgtgccggc
     1261 gatgcagcgt ggtcatgcgt ttgcgttcgg agtcgaacgg cagctccatg tcgcgcggtg
     1321 ccgcggcttc cagtctgtcc ttgtcgtaac ccgcatcca
//
LOCUS       DASRVO010000063         1151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_131404, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000063 DASRVO010000000
VERSION     DASRVO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131404"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..509)
                     /locus_tag="VFF05_00745"
     CDS             complement(63..509)
                     /locus_tag="VFF05_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZW50362.1"
                     /translation="MWAMGPANRDATMNQGVRFSDLMSRLAVTALAVVLAIAIEGLLE
                     SAKVSAPYLVFLPIITGVCAISGFAMGLCAIFFSTLGLVYFFIPPDGFDLPSSADFMH
                     LCVFITVAFFGCWIIDGLKRTNDELSRDNFVLGCKVSILMSRKKSR"
     gene            632..>1151
                     /locus_tag="VFF05_00750"
     CDS             632..>1151
                     /locus_tag="VFF05_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZW50363.1"
                     /translation="MSDAPKRNSFECYWMPFTANRQFKAQPRLLESAEGMYYRSSDGR
                     RILDGTAGLWCVNAGHGRREIAEAVHRQVLTLDYAPTFQMGHPAAFEAASRIAALMPG
                     DLDHVFFGNSGSEAVDTALKIALAYHRMRGEGGRTRLIGRERGYHGVGFGGVSVGGIA
                     ANRKLYAGIPGVD"
BASE COUNT          228 a          362 c          362 g          199 t
ORIGIN      
        1 ggcggtctcc gcgcgatcga tgcagaacgg ggcgatgaga gaagcaggct tccgccgatc
       61 catcagcgcg acttcttgcg gctcatcaga atcgagactt tgcagccgag cacgaaattg
      121 tcgcgcgata gctcgtcatt cgtgcgcttc agcccgtcga tgatccagca gccgaagaac
      181 gccacggtga tgaacacgca gagatgcatg aaatccgccg agctcggcag atcgaatccg
      241 tcgggcggaa tgaagaaata aaccaggccc agcgtgctga agaaaatcgc gcaaagaccc
      301 atggcgaagc cgctgatggc gcatacgccg gtgatgatgg gaaggaagac gagatagggc
      361 gcggagacct tcgccgattc gagcagccct tcgatcgcga tcgccaacac cacggccagc
      421 gccgtgacgg ccagccgcga catgagatcc gaaaagcgga ctccctgatt catggtagca
      481 tctctgttcg ccggtcccat cgcccacatg tgatcatgct tcttttatct tgtccagccc
      541 gagcggctca gccgcgccga agcatcatcg caagcgccgc gcacggcgtg ctacgctcgc
      601 ctccatttca ggatttgcgg gcgagaacgc catgagcgac gcgccgaagc gaaacagttt
      661 cgagtgttat tggatgccct tcaccgcgaa ccgccagttc aaggcgcagc cgcgcctgct
      721 ggaatcggcc gagggcatgt attatcgcag cagcgacggc cggcgcattc tcgacggcac
      781 cgcgggattg tggtgcgtca atgccggcca tggacgccgc gagatcgcgg aggcggtgca
      841 tcgccaagtt ctgacgctcg attacgcgcc gacgtttcag atgggccatc ccgccgcgtt
      901 cgaggcggcc tcgcgcatcg ccgcgctcat gcccggtgat ctcgatcacg tcttcttcgg
      961 caattccggt tccgaagcgg tcgacaccgc gctcaagatc gcgctcgcct atcatcgcat
     1021 gcgcggcgaa ggcgggcgca cgcggctgat cgggcgcgag cgcggctatc acggcgttgg
     1081 tttcggcggc gtatcggttg gcggcatagc ggcgaaccgc aagctctatg ccggcatccc
     1141 gggcgtcgat c
//
LOCUS       DASRVO010000064         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_261479, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000064 DASRVO010000000
VERSION     DASRVO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261479"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..837)
                     /locus_tag="VFF05_00755"
     CDS             complement(40..837)
                     /locus_tag="VFF05_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW50364.1"
                     /translation="MNTLVRPLESLGRATLGALANVGYAAMLLLESLYFSCAGWRIGQ
                     PLRLRAVAEQMRQIGVDALPIVTLLALTIGVMLGIQFIAALSEFGAQSQVVVAVAKSV
                     TREFGALITAILVAGRSGSALAARIGSMSVSQEVDALATMGIEPVRYLAAPALLAMLV
                     MLPMLTIFADAVAIAGAALYASPALNVTPTVYVMQTLALLAPGDIGQGIAKSAVFAVL
                     ITLIGVSTGFSVQGGAEGVGRATTRAVVLSICWIIVVDMIFSFFLNR"
     gene            complement(839..1177)
                     /locus_tag="VFF05_00760"
     CDS             complement(839..1177)
                     /locus_tag="VFF05_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZW50365.1"
                     /translation="MNATANACESENLDDYTLVRVRGEVDLSWSQQVRDAILAALGRG
                     GVGVELSQVQYIDSSGIAALVEGFQRARGTRRRFGLIASSKQVLAVLQLARLDKVFPL
                     YVDVDAARSA"
     gene            complement(1174..>2413)
                     /locus_tag="VFF05_00765"
     CDS             complement(1174..>2413)
                     /locus_tag="VFF05_00765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZW50366.1"
                     /translation="AAAIVFTALLAATLAEADRQRRLLREAQARVAGELEAARRIQMG
                     LLPAPRELFAQEQRFSLAALLEPARTVGGDFFDCFMLDHDRLFFLVGDVSGKGLDAAF
                     LMVRVASMLRWVGAQAPAPGAWLAQVNVELCRTLQDGRFVCAAVGQYDRASGKVRLAS
                     AGFPPVLLHDGIDFEELAADGPPLGILDDADYGEREFALGQSALYLFSDGATDVRTGA
                     TDRLGESGLRGLIARHATLAPEPRLRAVIGDLKRLHLVDDTTLLLLQEPCGTQAHTLL
                     DLEFPAQAAQMRSVRAALRHTLDAQGVAPEMRDQLVLAVDEACTNVIRHAYGGECTRR
                     IALHLARERDMLAFELRDQAPAVDPACLKPRDLSECKPGGLGLPFIAATMDDWKLEPA
                     ADGYGNILRMHKRIDTGSTE"
BASE COUNT          440 a          820 c          807 g          346 t
ORIGIN      
        1 cttcgatcag cggcgcggac gcggtcgtca tggatcgaat cagcgattca ggaaaaacga
       61 gaaaatcatg tcgaccacga tgatccagca gatcgacaga accaccgcgc gcgtggtcgc
      121 gcggcccacg ccctccgcac cgccctgcac cgagaatccc gtgctcacgc cgatcagggt
      181 gatcagcacg gcgaacaccg cggacttggc gatgccctgc ccgatgtcgc ccggcgccag
      241 cagcgcgagg gtttgcatca cgtagaccgt cggcgtgacg ttgagcgcgg gcgaggcgta
      301 caacgccgca ccggctatcg cgaccgcatc ggcgaagatc gtcagcatcg gcagcatgac
      361 cagcatggca agcaaagccg gcgcggcaag gtaacgcacc ggttcgatgc ccatcgtggc
      421 cagcgcatcg acttcctgcg acacgctcat cgaaccgatg cgcgcggcga gcgccgaacc
      481 cgagcgcccg gcaacaagga tcgcggtgat cagcgcgccg aattcgcgcg tcaccgattt
      541 cgctaccgcg acgaccacct gcgactgcgc accgaattcg ctcaatgcgg cgatgaactg
      601 aatgcccagc atcacgccga tggtcaacgc gagcagggtg acgatgggca gcgcatccac
      661 cccgatctgg cgcatctgtt cggcgaccgc gcgcaggcgc agcggctggc cgatgcgcca
      721 gccggcgcag gaaaaataca ggctttcgag cagcagcatt gccgcatagc cgacattggc
      781 gagcgcaccc agcgtggcgc ggccaaggct ttcgagcggg cggacgaggg tattcatgtc
      841 atgcgctgcg ggcggcatcc acatccacgt acaacggaaa caccttgtcc agccgtgcca
      901 gttgcagcac cgccagaacc tgcttgctcg acgcgatcag gccgaaacgc cggcgcgtgc
      961 cgcgcgcgcg ctggaagcct tcgaccagcg cggcgatgcc ggacgaatca atgtactgca
     1021 cctgcgacaa ttccacgccg acgccgccgc ggccgagcgc cgcgagaatc gcgtcgcgca
     1081 cctgctgcga ccaggacaga tcgacctcgc cacgcacgcg cacgagcgtg tagtcgtcga
     1141 gattttcgct ctcgcaggcg ttggccgtag cgttcattcc gtgctccccg tgtcgatgcg
     1201 tttgtgcatg cgcaggatat tgccgtaccc gtccgccgcc ggttccagtt tccagtcgtc
     1261 catcgtcgcg gcgatgaacg gcaggccgag tccaccgggt ttgcattcgg acaggtcgcg
     1321 cggtttcagg caggccgggt cgacggccgg cgcctggtcg cgcaactcga acgcgagcat
     1381 atcgcgctcg cgggcaaggt gcagcgcgat gcggcgcgtg cattcgccgc cgtaggcatg
     1441 gcggatgacg ttggtgcagg cctcgtccac ggcgagcacg agctgatcgc gcatttccgg
     1501 cgcgacgccc tgggcatcga gggtgtggcg caacgccgcg cgtacgctgc gcatctgcgc
     1561 cgcctgcgcg ggaaattcca ggtccagcag ggtgtgcgcc tgcgtgccgc agggttcttg
     1621 caacagcaac aatgtcgtgt cgtcgaccag gtgcagacgc ttgagatcgc cgatgaccgc
     1681 gcgcaaacgc ggttcgggcg cgagcgtcgc gtggcgcgcg atcaggccgc gcaggccaga
     1741 ttcgccgaga cgatccgtcg cgccggtgcg cacgtcggtg gcgccgtctg aaaacaggta
     1801 aagcgcgctc tggccgagcg cgaattcgcg ttcgccgtaa tccgcgtcgt cgaggatgcc
     1861 gagcggcggg ccgtcggcgg cgagttcctc aaagtcgatt ccatcgtgca gcagcaccgg
     1921 cggaaatccc gcgctggcca ggcgaacctt gccactggcg cggtcgtact ggccgactgc
     1981 ggcacagacg aaacggccgt cctgcagagt acggcacaac tctacattca cctgcgccag
     2041 ccacgcgccg ggcgccggtg cctgcgcgcc aacccagcgc aacatgctgg ccacgcgcac
     2101 catcaaaaac gccgcgtcca ggcccttgcc cgagacatcg ccgacgagaa agaacaggcg
     2161 atcgtggtcg agcatgaagc agtcgaagaa gtctcccccg accgtgcgcg cgggctcgag
     2221 cagggcagcg agggagaagc gctgctcctg ggcgaagagc tcgcggggtg cgggcagcag
     2281 gcccatctga atgcgtcgcg ccgcctcgag ctcgccggcg acgcgcgcct gcgcctcgcg
     2341 cagcagccgg cgctgccgat cggcttcggc cagcgtggcg gcaagcagcg cggtgaagac
     2401 gatggctgcg gcg
//
LOCUS       DASRVO010000065         1106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_287583, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000065 DASRVO010000000
VERSION     DASRVO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287583"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFF05_00770"
     CDS             complement(<1..259)
                     /locus_tag="VFF05_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50367.1"
                     /translation="MWIFHRFSGLANGTGANPADVVALVLHLTHAERSAGVSVASQLV
                     LIVDDRITNLKILERLATSLGEEIASRTFGDPFEALAAVARE"
     gene            complement(373..>1106)
                     /locus_tag="VFF05_00775"
     CDS             complement(373..>1106)
                     /locus_tag="VFF05_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HZW50368.1"
                     /translation="DPQVAFHAVRQLAHLAGNVANIRWVQAGFLSMPGGGATPRNLMG
                     FRDGTANPRPTDPATMTKFVWIGDGPDWLRGGSYLVARRIRIALEHWDRMKLAFQEQT
                     IGRHKYSGAPLGCAHEFDKLDLDATDKDGNPIIPENAHVRLAAAASNDGAQILRRPYS
                     YNDGANVTAERWPPWRAGLEYDAGLFFLCYQRDPRDGFIPIYANMAKLDMMNQFVTHV
                     GSGVFVCPRGVAPGEFVGQGLFETA"
BASE COUNT          187 a          352 c          376 g          191 t
ORIGIN      
        1 gttcccgggc gacggcggca agtgcctcaa acgggtcgcc aaaggtccgc gacgcaattt
       61 cctcaccgag cgaggtcgcc agcctttcga ggatcttcag gttggtgatg cggtcgtcga
      121 cgatgaggac cagttgcgaa gccacagaaa ccccggccga acgttctgcg tgtgtcaagt
      181 gtaataccaa cgccaccaca tcggcgggat ttgcgccggt gccgttcgcc agcccagaaa
      241 agcggtggaa aatccacact tggcgcgccg cacttccctc cgctttgacg cggcgatcgg
      301 catggttgtc gcgaatcgct tgtcaccggg ggctgcgggc tctcgcgccg ggtatcggga
      361 tcgctgcggc ccctatgccg tctcgaacag cccctggccg acgaattcgc ccggcgcgac
      421 accgcgcgga cagacgaaca cgccgctgcc gacatgggtg acgaactggt tcatcatgtc
      481 gagcttggcc atgttcgcgt agatcgggat gaaaccgtcg cgcgggtcgc gctggtagca
      541 caggaaaaac aggcctgcat catattcaag ccccgcacgc cagggcggcc agcgctcggc
      601 ggtgacattg gccccgtcgt tgtaggaata gggtcggcgc aggatctgcg cgccgtcatt
      661 gctggcggcg gcggcaagcc ggacatgcgc gttttccggg atgatcgggt tgccgtcctt
      721 gtcggtcgcg tcgaggtcga gcttgtcgaa ttcatgagcg cagcccagcg gcgcccccga
      781 atatttgtgg cggccgatcg tctgctcctg gaaggcgagc ttcatgcggt cccaatgctc
      841 aagcgcgatg cggatccggc gcgccacgag atagctgccg ccgcgcagcc agtcaggccc
      901 gtcgccgatc cagacgaact tggtcatcgt cgccggatcg gtgggtcgcg gattggcggt
      961 gccgtcgcgg aagcccatca ggttgcgcgg cgtcgcgccg ccgccgggca tcgagaggaa
     1021 gccggcctga acccaacgga tgttggcgac gttcccggcg aggtgcgcca gctggcgcac
     1081 cgcgtggaag gccacctgcg gatcgt
//
LOCUS       DASRVO010000066         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_157625, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000066 DASRVO010000000
VERSION     DASRVO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157625"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFF05_00780"
     CDS             complement(<1..447)
                     /locus_tag="VFF05_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50369.1"
                     /translation="MAAAIEHGLRPLTPAVPALFGTRGEIFWERQFGGVEHALGNYDL
                     AETHWQRAQRLALAHGELGTAAQILKLRGEQAAVSDYARTERIAEELSQLAERARLPS
                     ARADAEYFLGIVDRRHGRLDSAAEHTQRALDAATALGDKRDEARALS"
     gene            complement(761..2857)
                     /locus_tag="VFF05_00785"
     CDS             complement(761..2857)
                     /locus_tag="VFF05_00785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZW50370.1"
                     /translation="MKTGLFLSRARWLAFAACLLSAAAFADASVAAAAYPRALAAADS
                     QRLAAAIERNVAPLTPAVPALVGAHGAIKFQRLLGGVFLALDAYANAENHLRRAHEIA
                     IAVGDAEDASAAAVDLGEIALLTGHYDRAEKLSNDLVQLATTANLPWAQAKAEEYLGV
                     VARRRGHLDDAQAHAQRALALQRALGQEAGTATALSNLGTLARDRGDYATALDLFRQA
                     LAIRERGDDQLELTLRNLALIYRDLGDDTAARDYFTRALDVARRHGDSANYAATLGTW
                     AGYLVDSDEFAPGLAAAEESLAVSGAIGDRPSVGFDLLNSGRALLGLGRLAEADTHLH
                     DALAIGRELNQHEIIAHSQVSLAEVALARGDAAQANKLLQAAFADPQTERYKPLMVEV
                     NELREKIATAIGKPAQALQFAHAAAALREDLLGTRASRRLSALESQYARKATEQQLAL
                     VTKDSQLQTERLTQLQLRRKYAIAALAGLSTLLALLAWRFAGVRRLNRALAVRNAEIE
                     SQRAALSHANDRLQQQADELFHAAISDPLTGVSNRGHLMRQLDARIADCARNGGELAA
                     LLIDFDHFKRINDAHGHLYGDRVLIAGVQALRQWLEPGDLLGRYGGEEFIVAVSGGDA
                     THAMRVAERLRERVAEAMTGFLPEANDAVTISIGIALLSRLSRPARLESLIEAADKAV
                     YAAKSAGRNRVVVHAA"
     gene            2958..>3975
                     /gene="rimO"
                     /locus_tag="VFF05_00790"
     CDS             2958..>3975
                     /gene="rimO"
                     /locus_tag="VFF05_00790"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZW50371.1"
                     /translation="MSQTSPKVGFVSLGCPKALVDSERILTQLRVEGYEIVPSYGTAD
                     VVVVNTCGFIDAAVTESLDAIGEALAENGKVIVTGCLGKRAEVIREAHPDVLAITGPQ
                     DYASVMRAVHKQVPRKHDPHLDLVPSQGIKLTPKHYAYLKISEGCNHRCTFCIIPSMR
                     GDLVSRPIDEVLIEAEKLVKSGVRELLVISQDTSAYGVDVKYAAREWRVKSYRTRMTE
                     LCAGLAELGAWVRLHYVYPYPHVDEVIPLMAEGKILPYLDIPFQHASPRILKLMKRPG
                     SSSKVLERIKAWRDVCPDLTIRSTFIVGFPGETQAEFEELLAFLDEAQLDRVGAFAYS
                     PVDGA"
BASE COUNT          650 a         1272 c         1356 g          697 t
ORIGIN      
        1 cgacagtgcg cgggcctcgt cgcgcttgtc gccaagggcg gtcgccgcat ccagcgcgcg
       61 ttgcgtgtgt tccgcggcgc tgtcgaggcg gccgtggcgg cggtcgacga tgccgaggaa
      121 atattcggca tcggcgcgtg cgctgggcaa cctggcgcgt tcggccagtt gcgacaactc
      181 ctcggcgatg cgttccgtgc gcgcgtaatc gctgaccgcg gcctgttcgc cgcgcagttt
      241 cagaatctgc gcagcagtgc cgagttcgcc atgcgcgagc gcaagccgct gcgcgcgctg
      301 ccagtgcgtt tcagcgagat cgtaattgcc cagcgcatgt tcgacgcctc cgaactggcg
      361 ctcccagaaa atttcgccgc gcgtgccgaa caaggccgga acggccgggg tcagcggacg
      421 cagaccgtgt tcgatcgcag cggccaagtg cagcgcctga atcgcggcct gcgcgtgggg
      481 gtaggtgtcg gtgtccggga cgctcgcgcc gcatgtgcat gcgcatgcgc aaaacaatgc
      541 ggccgccaat ccgaagcgac atcgaagccg cccgtatgca ttcgtcctgc cggaatgtct
      601 cacatgactt cctcgtgcga ttcccctgcg aaccgccatc gccgacaagt gtagttcaca
      661 tggctttaca tcggcaaaaa cccacgttca ggatttgcat cgatcgttcg cgccaagctg
      721 cgcggctgct ggcacgcgcg atgccgagcg cagcgggggt tcaggcggcg tgcacgacca
      781 cgcggttgcg tccggccgat ttcgccgcgt acaccgcctt gtcggcggct tcgatcaggg
      841 attccagccg cgcgggcctg gataaacgcg aaagcaaggc gatgccgatg ctaatcgtca
      901 ccgcgtcgtt cgcctcggga agaaagcccg tcattgcctc ggcgacgcgt tcgcgcaggc
      961 gctcggccac ccgcatggca tgcgtcgcat cgccgccgct gaccgcgacg atgaattcct
     1021 cgccgccgta gcgcccgagc agatcgccgg gctccagcca ttggcgcagg gcttgtacgc
     1081 cggcgatcag cacgcggtcg ccgtacagat gtccgtgcgc gtcgttgatg cgcttgaagt
     1141 gatcgaaatc gatcagcagc gccgccagtt cgccgccgtt gcgtgcgcag tcggcgatgc
     1201 gcgcatcgag ctggcgcatc agatggccgc gattggatac gccggtgagc ggatcgctga
     1261 tcgccgcatg gaacagttcg tcggcctgct gctgcaggcg gtcgttggcg tggctcaagg
     1321 cggcacgctg cgattcgatc tcggcgttgc gcacggcgag agcgcggttg aggcgacgca
     1381 cgccggcgaa gcgccacgcc agcagtgcca gcagtgtgct gagtccggca agcgcggcga
     1441 tggcgtattt gcgccgcaac tgcaattgcg tcaggcgctc ggtttgcaat tggctgtcct
     1501 tggtgaccag cgcgagttgc tgctcggtgg ccttgcgtgc gtactggctt tccagcgcgg
     1561 acaggcgtcg gctcgcgcgc gtgccgagca agtcttcgcg cagcgcggcg gcggcatgcg
     1621 cgaattgcag cgcctgcgcg ggcttgccga tggcggtggc gatcttctcg cgcaattcgt
     1681 tcacttcaac catcaacggc ttgtagcgtt cggtctgcgg gtcggcgaac gcggcttgca
     1741 gcaatttgtt tgcctgtgcc gcgtcgccgc gcgcgagcgc gacttcggcc agcgagacct
     1801 ggctgtgtgc aatgatctcg tgctggttca gttcgcgacc gatcgccagc gcgtcgtgca
     1861 gatgcgtgtc ggcttcggcg aggcggccca ggccgagcag ggcgcgaccg ctattgagca
     1921 gatcgaagcc gaccgacggg cggtcgccga tggctccgct cactgccagc gattcttccg
     1981 cggccgcgag tcccggcgcg aattcgtcgg aatcaaccag atatccggcc caggtgccga
     2041 gtgtggcggc gtagttggcg ctgtcgccgt ggcggcgtgc gacgtcgagc gcgcgcgtga
     2101 aatagtcgcg tgcggcggta tcgtccccga ggtcgcgata gatcagcgcc aggttgcgca
     2161 gggtcagttc gagctggtcg tcgccgcgtt cgcgtatcgc cagcgcctgc cggaacaggt
     2221 cgagcgcggt ggcgtagtcg ccgcggtcgc gtgcgagcgt gccaaggttg gacagcgccg
     2281 tggcggtacc ggcctcctga ccgagcgcgc gctgcagcgc cagtgcgcgc tgcgcgtgcg
     2341 cttgcgcatc gtcgagatgg ccgcgacggc gcgcgaccac gcccaggtat tcttcggcct
     2401 tggcctgcgc ccatggcagg ttcgcggtcg tggccagttg gaccaggtcg ttcgacaatt
     2461 tttcggcgcg gtcgtagtgg ccggtcagca gcgcgatttc gccgagatcg acggcggccg
     2521 cggacgcatc ctcggcgtcg ccgacggcga tcgcgatttc atgcgcgcgg cgcaaatgat
     2581 tttccgcatt ggcgtaggca tccagcgcca ggaaaacccc gccgagcaag cgctggaact
     2641 tgatcgcgcc gtgcgcgccg acgagagcgg ggacggccgg tgtcagcggc gcgacgttgc
     2701 gttcgatggc ggcggcgagg cgctgcgaat cggcggccgc caacgcgcgc ggataggccg
     2761 cggcggcgac cgaggcgtcg gcgaacgcgg cggcggacag caggcatgcc gcaaacgcca
     2821 gccagcgcgc gcgactcaag aacaggccag tcttcatcgg atcagccctg tacgctgcga
     2881 ctgcggtttc ccagcgaaac cgtaaaccgg ccaagtgtaa actacgcgcc cttgccggag
     2941 caaaaagcgg ttcgcgcatg tcccaaacct cgcccaaagt cggtttcgtc agcctcggct
     3001 gccccaaggc gctggtcgat tccgagcgca tcctcacgca actgcgcgtg gagggctacg
     3061 agatcgtgcc cagctacggc acggcggacg tcgtcgtcgt caacacctgc ggtttcatcg
     3121 acgcggcggt gaccgagtcg ctggacgcga tcggcgaggc gctggccgag aacggcaagg
     3181 tcatcgtgac cggctgcctg ggcaagcgcg ccgaggtgat ccgcgaggcc catcccgacg
     3241 tgctcgccat caccggtccg caggattacg cgagcgtgat gagagcggtg cacaagcagg
     3301 tgccgcgcaa gcacgatcca cacctcgatc tggttccgtc acaaggcatc aaactcacgc
     3361 cgaaacatta tgcctatctg aaaatttcgg aaggatgcaa tcacagatgc acgttctgca
     3421 tcattccgtc gatgcgcggc gatctggttt cgcgcccgat cgacgaggtg cttatcgaag
     3481 ccgagaaact ggtcaagtcc ggcgtgcgcg aattgctcgt catctcgcag gataccagcg
     3541 cctacggcgt ggacgtgaaa tacgcggcac gcgaatggcg cgttaaatcg tatcgcacgc
     3601 gcatgacgga actgtgcgcg ggacttgccg agttgggcgc gtgggtgcgc ctgcactatg
     3661 tgtatccgta tccgcacgtg gacgaggtga ttccgctgat ggcggaaggc aagatcctgc
     3721 cgtatctcga cattcccttt cagcacgcca gcccgcgcat cctcaagctg atgaagcgcc
     3781 cgggttccag cagcaaagtg ctcgagcgca tcaaggcgtg gcgagacgtg tgtccggatc
     3841 tcacgatacg cagcaccttc atcgtcggct ttcccggcga gacgcaagcc gagttcgagg
     3901 aactgctcgc gttcctggat gaggctcaac tcgaccgcgt cggcgcgttt gcctactcgc
     3961 cggtcgatgg cgcca
//
LOCUS       DASRVO010000067         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_235321, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000067 DASRVO010000000
VERSION     DASRVO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235321"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFF05_00795"
     CDS             complement(<1..374)
                     /locus_tag="VFF05_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZW50372.1"
                     /translation="MPSAPATTETADAPMLVNCVAYTEGKPRTIALDDIGEALKDPKV
                     FVWVGLHEPDESMLDRLQAQFGLHPLAIEDAHRAHQRTKIEVYGDSLFVAVHTAQMAH
                     SKVDLGETHLFLGRNYLVSVRHG"
     gene            complement(421..>1554)
                     /locus_tag="VFF05_00800"
     CDS             complement(421..>1554)
                     /locus_tag="VFF05_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HZW50373.1"
                     /translation="DLDVALVTFGPGTELWERFGHNAILIRDRTSGRQRLYNYGMFDF
                     AQKDFFLNFARGRMLYRIAVDDPADEFPFYRSEGRWIVEQDLNLTPEQRVKLFDYLEQ
                     NIRPENAQYRYDYFTANCATRVRDALDTALDGAIRTQLIAPSRGFTYRMDSMRLMRPE
                     PVLMVLMDASLGPFADKRISYWAESFVPMEFMRYVRDLKVRDAAGNLVPLVVRETMLD
                     AGRLPEPPEYPSNWVWQALAIGVAAGLGLLALARRRERAWARATFAASASLIAFACGS
                     AGLVLIGLWTLTDHVAAWHNENILLLNPLCLLLMPAWIASARARWQPSRFIKAMSFLV
                     AFVAAFEFFLKIYPSFPQDNRFWIALLLPIHAALAASVNWKRS"
BASE COUNT          278 a          466 c          544 g          266 t
ORIGIN      
        1 ccgtggcgga cgctgaccag gtaattgcgg ccgaggaaca gatgcgtctc gcccagatcc
       61 accttgctgt gcgccatctg cgccgtgtgc acggcgacga acagcgaatc gccgtagacc
      121 tcgatcttgg tgcgctgatg cgcgcggtgc gcgtcctcga tcgccaatgg atgcaagccg
      181 aactgcgctt gcagccggtc gagcatggat tcgtccggct cgtgcagccc cacccacacg
      241 aacaccttgg gatccttcag cgcttcgccg atgtcgtcca gtgcgatcgt gcgcggcttg
      301 ccttcggtgt acgcgacgca gttgaccagc atcggcgcat cggcggtttc agtcgtggcg
      361 ggcgcgctcg gcatgcgcgg atcatgccgt agcggggcaa gcggggcaat gggtgcgcga
      421 ctagctgcgc ttccaattca cactcgccgc cagcgccgcg tggatcggca gcagcagcgc
      481 gatccagaag cggttgtcct gcgggaatga ggggtagatt ttcaggaaga actcgaacgc
      541 ggcgacgaag gcgacgagga agctcattgc cttgatgaag cgcgacggtt gccagcgcgc
      601 gcgtgcgctg gcgatccacg cgggcatgag gagcaagcac agcggattga gcagcaggat
      661 gttttcgttg tgccaggcgg cgacgtggtc ggtgagcgtc cacaggccga tcagcacgag
      721 tccggcgctg ccgcaggcga aggcaatcag actcgcactg gcggcaaaag tcgcgcgtgc
      781 ccacgcgcgc tcgcgccggc gtgcgagcgc gagcaggccg agtccggcgg cgacgccgat
      841 ggcgagcgcc tgccagaccc agttcgacgg atattccggc ggttcgggca gacgaccggc
      901 gtcgagcatg gtttcgcgca cgaccagcgg cacgaggttt ccggcggcgt cgcggacttt
      961 caggtcgcgc acgtagcgca tgaattccat cggcacgaaa ctctcggccc agtacgagat
     1021 ccgcttgtcg gcgaagggac cgaggctggc gtccatcagt accatgagca ccggttccgg
     1081 acgcatcagg cgcatgctgt ccatgcggta ggtgaagccg cgcgagggcg cgatgagctg
     1141 cgtgcggata gcgccgtcga gcgcagtgtc gagcgcgtcg cgcacgcgcg tggcgcagtt
     1201 ggcggtgaag tagtcgtagc ggtactgcgc gttttccggg cgaatgttct gttcgagata
     1261 gtcgaacagc ttcacgcgct gttccggcgt gaggttcaga tcctgctcga cgatccagcg
     1321 gccttcactg cgatagaacg gaaattcgtc ggccggatcg tccacggcga tgcggtagag
     1381 catgcgtccg cgcgcgaagt tgaggaagaa atccttctgc gcgaaatcga acatgccata
     1441 gttgtacagg cgctgcctgc cgctggtacg gtcgcgaatc aggatggcgt tatggccgaa
     1501 gcgctcccac agttcggtgc cggggccgaa ggtgaccagg gcaacgtcca agtc
//
LOCUS       DASRVO010000068         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_261660, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000068 DASRVO010000000
VERSION     DASRVO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261660"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFF05_00805"
     CDS             <1..500
                     /locus_tag="VFF05_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50374.1"
                     /translation="GNRLFANYGINIDLNHDGPTANDAADADNGPNGLQNFPLLLSLS
                     HTGDVRKINGTLDVSASSVVIEFFGAPSCGAAGRGDAAQALSVGTRPILVFNPGVISF
                     SREVPRGPGVDADAYCRISATATAFDTANPGNNKNTSELSACFVDDTIFAHSFDAQAG
                     WSCAP"
     gene            complement(527..>1307)
                     /locus_tag="VFF05_00810"
     CDS             complement(527..>1307)
                     /locus_tag="VFF05_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZW50375.1"
                     /translation="RLGATLCHRGRSGFVLTDEGTCVFDAAGRLLNAVSQFHTEVEEI
                     NDRLRGRLRLALFDKTASNPGSRIAIALGDFAKRAPAVELELHVAPTHQIEAGVIDGA
                     WQFGIIPTYRPSPALDYLPLFDEQMYLYCGREHPLFAHRDVRGAERVVWSQRYAGLSF
                     HSPNMDTGNRFEFTRSAEASDQEAIATLVLSGQFIGFLPDHYAAPFEAQGLMRALYPK
                     RFQYTCQFAAIYRRAPPPSRLLEIALASLRRAHAQVTRRAR"
BASE COUNT          229 a          398 c          457 g          223 t
ORIGIN      
        1 cgggcaatcg cctgttcgcc aattacggca tcaatatcga cctcaaccac gacggcccga
       61 ccgcgaacga tgcggctgac gctgacaacg gtccaaacgg attgcaaaat ttcccgctgt
      121 tgctgtcgct gagccacacc ggtgacgtgc gcaagatcaa tggcacgctg gacgtcagcg
      181 cgagcagcgt cgtgatcgag ttcttcggcg cgccgagttg tggcgctgcc ggacgcggcg
      241 acgcggcgca ggcgctcagc gtggggacgc gaccgattct cgttttcaat cctggcgtca
      301 tctcgttctc gcgcgaggtg ccgcgcggcc cgggcgtgga cgcggacgcc tactgccgca
      361 tcagcgcgac ggcgaccgct ttcgacaccg cgaatccagg caacaacaag aatacctcgg
      421 agctctccgc gtgcttcgtc gatgacacga tcttcgcgca cagtttcgat gcgcaggctg
      481 gttggtcgtg cgcaccttga tggcgtgaac gggtcgaatc gcccgctcag cgtgcgcgac
      541 gcgtgacctg cgcgtgcgcg cggcgcaggc tggcgagggc gatttcgagc agtcgcgatg
      601 gcggcggcgc gcggcggtag atggcggcga attggcaggt gtattggaaa cgcttcgggt
      661 acagcgcgcg catcaggccc tgtgcctcga acggcgcggc atagtggtcg ggcagaaagc
      721 cgatgaattg gccggatagc accagcgtgg cgatggcttc ctgatcggag gcttcggccg
      781 agcgggtaaa ttcgaagcgg ttgccggtat ccatgttcgg cgaatgaaag ctcaggcccg
      841 cgtagcgctg cgaccagacg acgcgttccg cgccgcgcac gtcgcggtgc gcaaacagcg
      901 ggtgctcgcg gccgcagtac aggtacatct gttcatcgaa cagcggcagg tagtcgagcg
      961 ccggcgaggg ccgatacgtc gggatgatgc cgaactgcca ggcgccgtcg atgacgccgg
     1021 cttcgatctg gtgggtcggg gcgacgtgca gttccagttc caccgccggt gcgcgcttgg
     1081 caaagtcgcc aagtgcaatc gcgatgcgcg agccgggatt gctggcggtc ttgtcgaaca
     1141 aggccagtcg caaacggcca cgcaggcggt cgttgatttc ctcgacttcg gtgtgaaact
     1201 ggctgaccgc gttgagcaga cgtccggcgg cgtcgaacac gcacgtgcct tcgtcggtca
     1261 ggacgaagcc cgagcgtccg cggtggcaca aggtcgcgcc gagacga
//
LOCUS       DASRVO010000069         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_183809, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000069 DASRVO010000000
VERSION     DASRVO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183809"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1528
                     /gene="hydA"
                     /locus_tag="VFF05_00815"
     CDS             80..1528
                     /gene="hydA"
                     /locus_tag="VFF05_00815"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020300566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HZW50376.1"
                     /translation="MTLLIRGGTVVNADQTSEADILCEGGTIRAVGKNLAAPANVDVV
                     DAKNCYVMPGGIDPHTHMELPFMGTVASEDFFTGTAAGLAGGTTMIIDFVIPNPRERM
                     LDAYKKWRGWAEKSCADYSFHVAVTWWDESVHNDMGTLVNECGVNSFKHFMAYKGSIM
                     ADDGVLVRSFPRALELGAIVTAHAENGDLVDHLQKEVFKRGVAGPEGHPASRPPEVEG
                     EAANRVIRIAQILGVPLYVVHNSCRESVEAIARARAQGQRVYGECLVQHLVIDESVYE
                     SKDWNFAAAHVMSPPFRPKSNQEALWDALRGGLLQTTATDHCCFCSEQKAMGRDNFAK
                     IPNGTPGIEDRLPILWHYGVNMGRLTPSEFVALTSTNTARIFNIYPRKGVLREGADDD
                     IVVWDPRRKKTISVETDHQKVDFNVFEGREVQGAPLYTISNGVVKYAEGKLHVEPGTG
                     RHIERPPFAPYLRAQQHWNDLNRIHPVDRAIV"
BASE COUNT          318 a          498 c          464 g          258 t
ORIGIN      
        1 catgcggatt ggaccaccca tcccaacaat gtattggcct gcgactaacg gtcaacgtca
       61 acccgggagg cgatgaacca tgacactctt gattcgcggc ggtaccgtgg tcaatgccga
      121 ccagaccagc gaagccgaca tcctgtgcga aggcggcacc atccgcgccg tcggcaagaa
      181 cctcgccgca ccggcgaatg tcgatgtcgt cgacgcaaaa aactgctacg tcatgcccgg
      241 cggcatcgac ccgcacaccc acatggaatt gccgttcatg ggaacggtcg ccagcgagga
      301 tttcttcacc ggcaccgcgg ccggcctcgc cggcggcacg accatgatca tcgatttcgt
      361 gattcccaat ccgcgcgagc gcatgctcga cgcgtacaag aagtggcgcg gctgggcgga
      421 aaagtcctgc gcggattatt ccttccatgt ggcggtgacg tggtgggacg agtcggtgca
      481 caacgacatg ggcaccctgg tcaacgagtg cggcgtcaac agtttcaagc atttcatggc
      541 ctacaagggt tcgatcatgg ccgacgacgg cgtgctcgtg cgcagcttcc cgcgcgcgct
      601 cgagctcggc gccatcgtca ccgcacacgc cgagaacggc gatctggtcg accatctgca
      661 gaaagaagtc ttcaagcgcg gcgttgccgg tcccgaaggc catcccgctt cgcgtccgcc
      721 ggaagtcgaa ggcgaggcgg ccaatcgcgt catccgcatc gcgcaaatcc tcggcgtgcc
      781 gctgtacgtg gtgcacaact cctgccgcga gtcggtcgag gccatcgcgc gtgcgcgtgc
      841 gcaaggccag cgcgtgtacg gcgaatgcct cgtgcagcat ctggtcatcg acgagtcggt
      901 gtacgaatcc aaagactgga acttcgccgc cgcccacgta atgagtccgc cgttccggcc
      961 gaaatccaat caggaagcgc tgtgggacgc gttgcgtggc ggcttgctgc aaactaccgc
     1021 caccgatcac tgctgcttct gcagcgagca gaaggcgatg ggccgcgaca acttcgccaa
     1081 gattccaaac ggcacgccgg gaatcgagga tcgcctgccg atcctgtggc attacggggt
     1141 caacatgggc cgcctgacgc cgagcgagtt cgtcgcgttg acctcgacga ataccgcgcg
     1201 catcttcaac atctacccgc gcaagggcgt actgcgcgaa ggcgccgacg acgacatcgt
     1261 ggtgtgggat ccgcgcagga agaaaaccat ctcggtcgag accgatcatc aaaaagtcga
     1321 tttcaacgtg ttcgaaggcc gcgaagtcca gggtgcaccg ctctacacca tcagcaacgg
     1381 cgtggtgaaa tacgccgaag gcaagctgca cgtcgagccc ggcacgggcc gccacatcga
     1441 gcgtccgccg tttgcgccgt acctgcgtgc gcagcaacac tggaacgatc tgaaccgtat
     1501 tcatccagtc gaccgcgcta tcgtctgaac catcgcgc
//
LOCUS       DASRVO010000070         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_183842, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000070 DASRVO010000000
VERSION     DASRVO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183842"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /gene="dapE"
                     /locus_tag="VFF05_00820"
     CDS             complement(<1..666)
                     /gene="dapE"
                     /locus_tag="VFF05_00820"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HZW50377.1"
                     /translation="MSDVLNLACELIRRRSLTPDDAGCQALIGERLQRAGFNIEHFHY
                     GDVSNLWATHGDGDPVFVFLGHTDVVPTGPESDWASAPFEPVIRDGRLYGRGAADMKS
                     GVAAMTVALEEFVAAHPDHAGTVALLLTSDEEGPLNRDGVRRVVGEFRRRGQRIDFCV
                     VGEPSSAERLGDIVRVGRRGSLSGNLSVHGVQGHVAYPDKVRNPIHIAAPALAELATT
                     RWDE"
     gene            complement(663..1016)
                     /locus_tag="VFF05_00825"
     CDS             complement(663..1016)
                     /locus_tag="VFF05_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HZW50378.1"
                     /translation="MLRIYGLEKCSTCEKARNWLDRKKIAHTFVDYREQRIDAATLKA
                     WAKAVGGWEKLVNRAGMTWRNLLPARKSPGSDAEWLLLIKEYPALVRRPVAVLDDGSV
                     HLGFTDKLYAKLFAA"
     gene            complement(1022..1852)
                     /gene="dapD"
                     /locus_tag="VFF05_00830"
     CDS             complement(1022..1852)
                     /gene="dapD"
                     /locus_tag="VFF05_00830"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZW50379.1"
                     /translation="MPDLRHIVEAAWERRAELAPANAGAELSAAVGQALDGVESGELR
                     VAEPDGAGGWRVNQWLKKAVLLSFRLNPNVVMDATPAPYFDKVPLRFEGFDAARFREV
                     GARVVPGAVVRRGAHIARDVVLMPSFVNIGAHVGAGTMVDTWATVGSCAQIGAHVHLS
                     GGVGIGGVLEPLQAAPTIIEDDCFIGARSEVVEGVIVERGSVIGMGVFLGQSTRIYDR
                     ATKQVHYGRVPAGSVVVAGALPAADGSHSLYAAVIVKRVDEKTRSKTSINELLRSGEE
                     "
BASE COUNT          335 a          635 c          590 g          324 t
ORIGIN      
        1 ctcgtcccag cgcgtcgtgg caagttccgc gagcgcgggc gcggcgatat gaatcggatt
       61 gcgcaccttg tccggatagg cgacgtggcc ttgcacgccg tgcacgctca ggtttcccga
      121 caacgagccg cgtcgaccca cgcgcacgat gtctccgagt cgttccgccg atgacggttc
      181 accgaccacg cagaaatcga tgcgttggcc gcggcggcgg aattccccga cgacacggcg
      241 gacgccatct cgattcagcg gaccttcttc gtcgctggtg agcagcaacg caacggtgcc
      301 ggcatgatcc ggatgcgcgg caacgaattc ctcgagcgcg acggtcatcg ccgccacccc
      361 ggatttcatg tccgccgcac cacggccgta tagacgtcca tcgcggatta ccggctcgaa
      421 tggcgcgctt gcccaatccg attccggacc ggtcggcacg acgtcggtgt ggccaaggaa
      481 cacaaacacc ggatcgccgt cgccgtgtgt cgcccacaga ttggaaacgt cgccatagtg
      541 aaaatgctcg atgttgaatc cggcgcgttg caggcgttcg ccgatcagcg cctggcagcc
      601 ggcgtcgtcc ggcgtcagcg agcggcgtcg gatcaattcg caggcgaggt tcagaacgtc
      661 gctcatgcgg cgaacagctt ggcgtaaagc ttgtcggtga agcccaggtg cacgctgccg
      721 tcatcgagca cggcgaccgg ccggcgcacc agtgccggat attccttgat cagcagcagc
      781 cactcggcat ccgagcccgg cgacttgcgc gcgggcagca ggttgcgcca ggtcatgccg
      841 gcgcgattga caagtttttc ccaaccgcca acggccttgg cccaggcctt gagcgtcgcc
      901 gcatcgatgc gttgctcgcg gtaatcgaca aaggtgtgtg cgatcttttt ccggtccagc
      961 cagttgcggg ccttctcgca ggtactgcat ttttccagtc cgtatatccg cagcatccga
     1021 atcactcctc gccactgcgc agcaactcgt tgatcgaggt cttgctgcgc gttttctcgt
     1081 ctacgcgttt gacgatgacg gcggcgtaca ggctgtggct gccgtccgcc gcgggcagcg
     1141 cgccggcgac gacgacgctg ccggccggca cgcggccgta atgaacctgc ttcgtggcgc
     1201 ggtcgtagat gcgcgtggac tgacccagga acacgcccat gccgatcacg ctgccgcgtt
     1261 cgacgatcac gccttcgacg acctccgaac gcgcgccgat gaagcagtcg tcctcaatga
     1321 tggtgggcgc ggcctgcaag ggttcgagca cgccgccgat gccgacgcca ccggacaaat
     1381 ggacgtgcgc gccgatctgc gcgcaggaac caaccgtggc ccaggtatcg accatggttc
     1441 cagcaccgac gtgcgcgccg atgttgacga agctcggcat gagcacgaca tcgcgcgcga
     1501 tgtgcgcgcc gcgcctcacg accgcgccgg gcacgactcg agcgccgact tcgcggaaac
     1561 gtgcggcatc gaagccttcg aagcgcagcg gcaccttgtc gaaatagggc gcgggcgtgg
     1621 catccatcac cacgttggga ttgagccgga acgacagcag caccgctttc ttgagccatt
     1681 gattgacacg ccagccgccc gcgccatcag gctcggcgac gcgcaattcg ccggattcga
     1741 ctccatccag cgcctgcccg accgcagcgg aaagctcggc gccggcattg gccggcgcga
     1801 gttccgcgcg ccgttcccac gccgcttcga cgatgtgccg cagatcaggc atgccgtttt
     1861 ccaaccattg aaaaatcgag aatg
//
LOCUS       DASRVO010000071         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_313623, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000071 DASRVO010000000
VERSION     DASRVO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313623"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VFF05_00835"
     CDS             <1..1393
                     /locus_tag="VFF05_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HZW50380.1"
                     /translation="SPGFDLPPFPKDAHVAQSVTVDGHTLKYTATVGSLPVRDEKGKK
                     IAEVVFTAYTMDGKDRPVTFALNGGPGASSVYLNFGAIGPKVVKFGAEGDNPSDPAVL
                     RENPGTWLDMSDLVFIDPVGTGFSRALVDDKQATTDFYSTNSDIHYLSRIIYDWLVKN
                     DRLQSRKYLIGESYGGFRGPRITEYLQTQLGVAMNGVVLLSPYLDPAASEDGDVSPLP
                     WMLTLPSIAAANLERTHKLSAATMQPVIDYTRGTYATTLMAGRTDPAAQEAMIKKVTE
                     LTGLDPVFVKRSGGRIETQAYLREIYRSEGKLGSRYDSNVTAWDPFPFAPQQETGDPL
                     LNGIIAPTTTAMVDFVTRIVGWKIDARYNALSYAVNRLWHQDEDARAGSVEQLRKAVA
                     NDPGLRVLIAHGWDDLSCPFMASVLIVDQMPAMGDVSRVQVKEYAGGHMFYARPDSQA
                     ELRADAMKLFGLR"
     gene            complement(1404..>2990)
                     /locus_tag="VFF05_00840"
     CDS             complement(1404..>2990)
                     /locus_tag="VFF05_00840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW50381.1"
                     /translation="AVARRVAQSAGSEGGGLVAMRFATGPNPETGDSLAASAVPSAAI
                     AIARQASTPRMAPCPCGSGRPFKQCCGSLRHAHSHGTGQYGTAAGEQTALLNRAIEHM
                     QRGEGAKAEAILGPLRAGAIDTSEIALAAGEICIDLHRLTEASAFLDRASVLAPDNPE
                     ICSAREECHRLGARMQASHGAGLGLRGQLERLALRARPAQAAGHVHIVSKLDAIGGTE
                     RRALNLYRCLSPHVPVTLWSSAPVAAPVAAEAPARMISSAAFPDGGALALVGTYFECG
                     GWLESSRFERVVICHNLAEQYGNLGRLLARLQDNDSRPRVELAFPSRYFRDLCGLPGT
                     VEYSPVDIAAFRPRQPSPESGRRLLVGRHGRANPYKFHPNDPAFFRGLLARGYAVRLL
                     GGSVVAAAFTGDAAKPELFDVGALGAADFLDSLDVFVYRKHPGLIETGGTVILEAMAA
                     GLPVVVFGEDCGNAEIVVDGENGFLVRDEAQANERIDRLRDDPALRIRLGRAARVTLV
                     DLMRRQEADIVRFYLERDAA"
BASE COUNT          523 a         1027 c          977 g          463 t
ORIGIN      
        1 aagtcccggc ttcgacctgc ccccgtttcc caaggacgcg cacgtcgccc aatcggtgac
       61 ggttgacggg cacacgctca agtacacggc cacggtcggc tcgctgccgg tgcgcgacga
      121 gaagggcaag aagatcgccg aagtcgtgtt caccgcctac acgatggatg gcaaggatcg
      181 cccggtcacg ttcgcgctca acggcggccc cggcgcctcg tcggtgtacc tgaactttgg
      241 cgcgatcggg ccgaaggtcg tcaaattcgg cgccgagggc gacaatccat ccgatcccgc
      301 cgtgctgcgc gaaaaccccg gcacctggct ggacatgtcg gacctggtct tcatcgaccc
      361 cgtcggcacc ggcttcagcc gcgcgctggt tgacgacaag caggcgacca cggatttcta
      421 ctcgacgaac agcgacatcc actatctgtc gcgcatcatc tacgactggc tggtgaagaa
      481 cgaccgcctg caatcgcgca aatacctgat cggcgaaagc tacggcggct tccgcggccc
      541 gcgcatcacc gaatacctgc agacgcaact gggcgtggcg atgaacgggg tggtcctgct
      601 ttcgccgtat ctcgatcccg ccgcgtcgga agacggcgat gtgtcgccgt tgccgtggat
      661 gctgaccctg ccctcgatcg cggccgcgaa cctggagcgc acgcacaagc tcagcgccgc
      721 gacgatgcaa cccgtcatcg actacacgcg cggcacttac gcgacgaccc tgatggccgg
      781 gcgcaccgat ccggccgcgc aggaggcgat gatcaagaag gtgaccgagc tgaccggcct
      841 cgatcccgtc ttcgtcaaac gctcgggcgg acgcatcgag acgcaagcct acctgcgcga
      901 gatctaccgc agcgagggca agctcggcag tcgctacgat tccaacgtca ctgcctggga
      961 tccgttcccg ttcgcgccgc agcaggaaac cggcgacccc ttgctcaacg gcatcatcgc
     1021 gccgaccacc acggcgatgg tggatttcgt cacccgcatc gtcggctgga agatcgacgc
     1081 gcgctacaac gcactcagct acgcggtaaa ccggctgtgg caccaggacg aagacgcgcg
     1141 cgcgggctcg gtcgagcaac tgcgcaaggc cgtggccaac gatccgggcc tgcgtgtgct
     1201 gatcgcgcat ggctgggacg acttgtcgtg tccgttcatg gcctcggttc tgatcgtcga
     1261 ccagatgccg gcgatgggcg acgtcagccg cgtgcaagtc aaggaatacg ccggcggcca
     1321 catgttctac gcgcggccgg acagtcaggc ggaattgcgc gcggatgcga tgaagttgtt
     1381 cggactgcgc tgaccggcgc aggtcacgcc gcatcgcgtt ccagatagaa gcggacgatg
     1441 tccgcttcct ggcgccgcat caggtccacc agagtcacgc gcgcggcacg gccaaggcgt
     1501 atccgcaacg cgggatcgtc gcgtaagcgg tcgatgcgct cgttggcttg cgcttcgtcg
     1561 cgcacgagaa aaccattttc gccgtcgacc acgatttcgg cattgccgca atcttcgccg
     1621 aacaccacga cgggcaggcc cgccgccatc gcctcgagaa tcaccgtgcc gccggtctcg
     1681 atcaaacccg ggtgcttgcg gtagacgaac acgtccagcg agtcgaggaa atccgccgca
     1741 ccgagcgcgc cgacgtcgaa cagctccggc ttcgccgcgt cacccgtgaa ggccgcggcc
     1801 acgacactgc cgccgagcag gcgcacggcg tagccgcgcg cgagcaagcc acggaagaat
     1861 gccgggtcgt tcggatggaa cttgtagggg ttggcccgcc cgtgccggcc cacgagcagg
     1921 cgccgacctg attccggtga cggttggcgc gggcgaaatg ccgcaatgtc gaccggcgaa
     1981 tactccaccg taccgggcaa tccgcaaaga tcgcggaaat accgcgatgg aaacgcgagt
     2041 tcgacgcgcg gccgtgagtc gttgtcctgc aagcgcgcca gcagtcgtcc gagattgccg
     2101 tattgctcgg cgaggttgtg gcagatcacg acgcgctcga acctggagga ttccagccag
     2161 ccgccgcatt cgaagtaggt tccgaccagc gccagcgccc cgccgtccgg aaaggccgcg
     2221 ctggaaatca tccgtgcggg cgcctcggcc gcgactggtg ccgcgaccgg cgcgctggac
     2281 cacagcgtga ccggcacgtg cggcgacagg cagcggtaga ggttcaaggc gcgccgctcg
     2341 gtgccgccga tggcgtcgag cttgctaacg atatgcacgt gcccggccgc ctgcgctggc
     2401 cgcgcgcgca gtgccaggcg ttcgagctgc ccgcgcaggc cgagtccagc gccatgcgat
     2461 gcctgcatgc gcgcgccaag ccggtggcac tcctcgcgcg cggaacaaat ctcgggattg
     2521 tccggagcca gcaccgatgc ccggtcgagg aatgccgatg cctcggtcag gcgatgcaga
     2581 tcgatgcaga tttcgccagc tgccagcgcg atctcgctgg tgtcgatcgc gcccgcgcgc
     2641 aacggcccga ggatcgcttc ggccttcgcg ccttcaccgc gctgcatgtg ttcgatcgcg
     2701 cgattgagca acgcggtctg ctcgccggcc gcggtgccat actgtccggt accgtggcta
     2761 tgcgcatggc gcagcgaccc acagcactgc ttgaacggcc tgccgctgcc gcacgggcat
     2821 ggcgccatgc gcggcgtgga cgcctgccga gcaatcgcga tcgctgcgct cggcacggcg
     2881 ctcgcggcga ggctgtcgcc ggtttccgga ttcggccccg tcgcgaaacg catggcgact
     2941 aggccgccgc cttcgctgcc tgcggattgc gccacgcggc gagcaactgc
//
LOCUS       DASRVO010000072         3630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_79841, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000072 DASRVO010000000
VERSION     DASRVO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79841"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFF05_00845"
     CDS             <1..481
                     /locus_tag="VFF05_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50382.1"
                     /translation="QTIRIARTGEDGVEDFLFWVFSGAPPGTGTSDESREPARWRSSA
                     FAALSTPVGRVLQVALKARRNGVDGIYAREAYQPALVTIAEVGITPGMAIDPLAPADS
                     TVSSIGIEREGFRNGNLVLTASMLYVDPLDPEVSVSWGGIYAGPVDLDKIFAHGFED"
     gene            508..876
                     /locus_tag="VFF05_00850"
     CDS             508..876
                     /locus_tag="VFF05_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50383.1"
                     /translation="MRYNKIATLLITGLLASVTWAQGGPPAPARVQAQSQTQAQTQTQ
                     AATRIGPGNTPGWSLMTPQERAEHRSKMMGFTNYEDCASYLAKHHAMMLERARNQGVT
                     LPAEPRPGFCQRLPHQGGSN"
     gene            993..2606
                     /gene="dnaX"
                     /locus_tag="VFF05_00855"
     CDS             993..2606
                     /gene="dnaX"
                     /locus_tag="VFF05_00855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004572793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZW50384.1"
                     /translation="MSYQVLARKWRPKKFSELVGQEHVVRALSNALDSQRMHHAFLFT
                     GTRGIGKTTIARIFAKSLNCERGQSSNPCGECAACRDIDAGRFMDMIEIDAASNTGVD
                     NVRDLIENAQYSPSRGRFKVYLIDEVHMLSKSAFNALLKTLEEPPPHVKFLLATTDPE
                     KLPVTVLSRCLKFNLKRLLPEQIVGQMRHILEAEQIAFDTEALDELARGADGSLRDGL
                     SLLDQAIAYGAGKVNAAEVRQMLGSIERGQVFALIESVQAGDGAKLMAQVEHLAEFAP
                     DFAGVLDDVAASLHRVQIVQLVGTADGDPRIAALADALGAEEVQLYYQIATTGRRDLP
                     IAPTPRAGFEMALLRMLAFRPADAKQVVAVRKPESTKAKPPESRSSQVSASRPEPVRE
                     APAAAPAGTHADVRQAADAGDWPALIAAAGLRGPIGQLAQHAALLGIDDGVVRLELKP
                     DHEHLRAPLLVTQLQDALGKALDRAVTVRFEKSAAAPRQTPADIARDQRSARQQAAEQ
                     ALEADPVVQGMIRDFGARVIPESIKAVEK"
     gene            2608..2931
                     /locus_tag="VFF05_00860"
     CDS             2608..2931
                     /locus_tag="VFF05_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZW50385.1"
                     /translation="MRGPMAQLMQQAQRMQDDLKRAQEELAKLEVTGQSGGGVVSVTM
                     NGRHEVRRVQIDRKLFADDPEMAEDLVAAAINDAVNKVAAASAQRMGEVTGGMNLPPG
                     FKLPF"
     gene            2950..3552
                     /gene="recR"
                     /locus_tag="VFF05_00865"
     CDS             2950..3552
                     /gene="recR"
                     /locus_tag="VFF05_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZW50386.1"
                     /translation="MSGSPLLNELIDALRCLPGVGAKTAQRMAFHVLERDRAGARKLA
                     EQLAAAVERIGNCTRCRTFSEEKICTLCASAARDAQTLCVVETPADQLAIEQATGFRG
                     RYFVLLGRLSPLDGMGPKELGLDLLAARLAEGEVRELIVATNPTVEGEATAHMLSQIA
                     RQAGVRATRLAHGVPLGGELEYIDRGTLAHAFGSRQNLED"
     gene            3557..>3630
                     /locus_tag="VFF05_00870"
     CDS             3557..>3630
                     /locus_tag="VFF05_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZW50387.1"
                     /translation="MGDTLFDKIIRREIPADIVYEDEE"
BASE COUNT          673 a         1165 c         1201 g          591 t
ORIGIN      
        1 gcagaccatt cggattgcgc gtaccggcga ggacggcgtg gaggatttcc tgttctgggt
       61 gttttccggc gcgcccccgg gcaccggcac ttcagacgag agcagggagc cggcacgctg
      121 gcggtcatct gcgttcgccg cgctgtccac gccagtcggc cgggtgttgc aggtcgctct
      181 caaggcacgg cgcaatggcg tagatggtat ttacgcgcgc gaagcctacc agcctgcact
      241 tgtcaccatc gccgaggtcg ggatcacgcc gggtatggcg atcgatccgc tcgccccggc
      301 cgattccacg gtcagctcga tcggcatcga gcgcgagggt tttcgcaacg gcaatctggt
      361 gctgactgcc agcatgctgt atgtcgaccc gcttgatccc gaggtttccg tcagctgggg
      421 cggcatttat gccggaccgg tcgatctgga caagatcttc gctcatggct tcgaagattg
      481 aggtcgtact tccagtgagg aggcatcatg cgctacaaca aaatcgctac gctgttgatc
      541 acaggcctcc tcgcatcggt gacgtgggcg cagggcgggc cgcctgcgcc cgcacgggta
      601 caggcgcagt cacaaacgca ggcgcagacg cagacgcagg cggcgacgag aatcggaccg
      661 ggaaataccc cgggctggtc gttgatgacg ccgcaggagc gcgccgagca ccgcagcaag
      721 atgatgggat tcacgaacta tgaggactgt gcgtcctacc tggcgaagca ccacgcaatg
      781 atgctggagc gggcccgcaa tcagggcgtg accctgccgg cggaaccgcg tccgggtttc
      841 tgccagcgtc ttccccacca aggcggttcg aactgaggtt cttcgcccga tggcggtgcg
      901 cggtcggcac cgccaccgtg cgccgtgcat ctgccgctgg ccgtccgcgg cccgacttgg
      961 cacaatcgac acttcggtag tccggtgtct cgatgagtta ccaagtccta gcgcgcaagt
     1021 ggcgccccaa gaagttttcc gagctggtcg gtcaggagca cgtggtgcgc gcgctcagca
     1081 atgctctgga ttcccagcgc atgcaccacg ccttcctgtt caccggcacg cgcggcatcg
     1141 gcaagaccac catcgcgcgc attttcgcca agtcgctgaa ctgcgagcgc ggccagtcgt
     1201 ccaatccctg cggcgaatgc gcggcctgcc gcgacatcga cgccggccgc ttcatggaca
     1261 tgatcgagat cgacgcggcc agcaacaccg gcgtcgacaa cgtgcgcgac ctgatcgaga
     1321 acgcgcaata ttcgccctcg cgcggccgct tcaaggtgta cctcatcgac gaggtgcaca
     1381 tgcttagcaa atccgcgttc aacgcgcttt tgaaaacgct ggaagaaccg ccgccgcatg
     1441 tgaagttcct gctcgcgacg accgatccgg aaaaactgcc ggtgacagtg ctgtcgcgct
     1501 gcctgaaatt caatctgaag cgcctgttgc ccgaacagat cgtcggacag atgcggcaca
     1561 tcctcgaggc cgagcagatc gccttcgata ccgaggcact ggatgaactc gcgcgcggcg
     1621 ccgacggatc gctgcgtgac ggactctcgc tgctggatca ggccatcgcc tacggcgcgg
     1681 gcaaggtgaa cgccgccgag gtgcgccaga tgcttggcag catcgaacgt gggcaagtat
     1741 tcgcgttgat cgaatccgtg caggcaggcg acggcgccaa gctgatggcg caggtcgaac
     1801 acctggccga gttcgcgccg gatttcgccg gcgtgctcga cgacgtcgct gcgtcgctgc
     1861 atcgcgtgca gatcgtgcaa ctggtcggca cggcggacgg cgacccgcgc atcgccgcgc
     1921 tggcggatgc gctcggcgcc gaggaagtgc agctttatta ccagatcgca acgaccgggc
     1981 gtcgcgacct gccgatcgcg cccacgccgc gtgccggatt cgagatggcc ttgctgcgca
     2041 tgctggcatt tcgcccggcg gacgcgaagc aggttgttgc agttcgaaaa cccgagtcga
     2101 caaaggcgaa gccacccgaa tcgcggtcgt cacaagtttc cgcatccagg ccggaaccgg
     2161 tgcgcgaagc gcctgcggcg gcgccggccg gcacgcatgc cgacgtacgg caggcggcgg
     2221 atgcgggcga ctggccggcc ttgatcgccg cggcaggttt gcgcggaccg atcggacaac
     2281 tcgcccagca cgcggccctg ctgggcatcg acgacggtgt cgtgcgcctt gaactcaaac
     2341 cggatcatga gcatctgcgc gcaccgttgc tggtgaccca attgcaggat gcgctcggca
     2401 aggcgctgga ccgcgcggtg acggtgcgtt tcgagaaatc cgccgccgcg ccgcggcaga
     2461 cgccggccga catcgcgcgc gaccagcgca gcgcacgcca gcaggctgcc gaacaggcgc
     2521 tggaagccga tccggtcgtg caaggcatga tccgcgactt cggcgcgcgc gttattcccg
     2581 aatcgatcaa ggcagtggag aagtgacatg cgcggaccga tggcgcaact gatgcaacaa
     2641 gcccagcgca tgcaggacga cctcaagcgc gcgcaggaag aactggccaa gctcgaagtc
     2701 accggtcaat ccggcggcgg cgtggtcagc gtgaccatga acggacgcca cgaggtgcgg
     2761 cgtgtgcaga tcgaccgcaa gctgttcgcc gacgatccgg aaatggccga agatctggtc
     2821 gccgccgcga tcaacgacgc ggtgaacaag gttgccgcgg caagcgcgca gcgcatgggc
     2881 gaggtgacgg gcgggatgaa cctgccgccg ggtttcaagc tgccgttttg atcagtatcg
     2941 attgattcga tgtccggatc gccgttgctc aacgaattga tcgacgccct gcgctgcctg
     3001 cccggcgtgg gcgcgaagac cgcgcagcgc atggccttcc atgtgctcga acgcgaccgt
     3061 gccggcgcgc gcaagctcgc ggaacaactc gctgctgcgg tcgagcgcat cggcaactgc
     3121 acgcgctgcc gcacattcag cgaggaaaaa atctgcacgc tgtgcgccag cgctgcgcgc
     3181 gatgcgcaaa ccctgtgcgt ggtggaaacg cccgccgacc aattggcgat cgaacaagca
     3241 accggtttcc gcgggcgtta tttcgtcctg ctcggacgat tgtcgccgct ggacggcatg
     3301 ggaccgaaag aactcggtct ggatcttctc gccgcgcgcc ttgccgaagg cgaagtgcgc
     3361 gaattgatcg tggcgacgaa cccgaccgtc gagggcgagg cgactgcgca catgctcagc
     3421 cagatcgcgc gccaggccgg cgtgcgtgcc acgcgcctgg cgcatggcgt gccgctgggc
     3481 ggcgaactcg aatacatcga tcgcggcacg ctcgcgcatg cgttcggatc gcggcaaaac
     3541 ctggaggact gaatccatgg gcgacacgct gttcgacaag atcatccgcc gcgagattcc
     3601 cgcggacatc gtctacgaag acgaggaaat
//
LOCUS       DASRVO010000073         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_105683, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000073 DASRVO010000000
VERSION     DASRVO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105683"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..790)
                     /locus_tag="VFF05_00875"
     CDS             complement(26..790)
                     /locus_tag="VFF05_00875"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZW50388.1"
                     /translation="MQPDSLYDLRGKTALVTGASRGIGLATAQLLAQCGAQVIVSSRR
                     QDACDTVAAQIAGTGGKAIAIAAHIGEAASIEALFADLDRCGLQLDILVNNAAANPYF
                     GPVLDMPMSAFDKTVEVNIRGYFLMSQLAARTMRERGGVIVNVASVNGRRAAPGQAVY
                     SMTKAAIVSMTESFARELAPVGIRVNAVLPGLTDTKFASALTGNPVILKPLLRAIPLG
                     RAAQPEEIAPMVTFLCSPAASYITGASFAVDGGYLT"
     gene            850..1653
                     /locus_tag="VFF05_00880"
     CDS             850..1653
                     /locus_tag="VFF05_00880"
                     /inference="COORDINATES: protein motif:HMM:NF004196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZW50389.1"
                     /translation="MDQKRVLITGAGSGLGRALAFCFAENGWRVACADIRLDRAQDTV
                     RLMRDFGVGAMALKVDVGSDASVEFMHDEILAAWDGVDVVVNNAGVASAGSVAQTSLD
                     DWRWTLNIDLMGVVRGCHAFLPVLLEQGSGHIVNIASFAGFANAPRMGAYSATKAGVI
                     SLSETLRAELANTAVKVSVACPAFFPTNLMDTARAPEADKATAKKFMAKATQSADDVA
                     AVIFREIQRGTFLILPTKGERGRWRIKRIAPELFFRKLVALAGATRIKP"
BASE COUNT          301 a          574 c          569 g          293 t
ORIGIN      
        1 ggccatccat ggccgcactt ctcgattagg tgagataccc accgtctacc gcgaagcttg
       61 ccccggtgat gtagctcgcc gctggcgaac acaggaacgt gaccatgggt gcgatctcct
      121 ccggctgcgc ggcgcggccg agcggaatcg cgcgcagcag cggtttgaga attaccggat
      181 tgccggtcaa tgcgctggcg aacttcgtgt cggtgagtcc cggcaacacc gcgttgacgc
      241 ggatgccgac gggcgccagt tcgcgtgcga aggattccgt catcgacaca atcgcggcct
      301 tggtcatcga ataaaccgcc tgccccggtg cggcgcgacg gccgttgacc gaagcgacgt
      361 tgacgatgac accgccgcgt tcgcgcatcg tgcgtgccgc cagttgcgac atcaggaaat
      421 agccgcggat gttgacctcg acggtcttgt cgaatgcgga catcggcatg tccagcaccg
      481 gcccgaagta aggattcgcc gccgcgttgt tgacgaggat gtcgagttgc aggccgcacc
      541 tgtccagatc ggcgaacagc gcctcgatgg atgccgcctc gccgatatgc gcggcgatcg
      601 cgatggcctt gccgccggtt ccggcgatct gcgccgcgac ggtgtcgcag gcgtcctgcc
      661 ggcggctgga cacgatcacc tgcgcaccgc actgcgcgag caattgcgcg gtcgccaggc
      721 cgatgccgcg gctggcgccg gtgaccagcg ccgtcttgcc gcgcaaatcg taaagggaat
      781 cgggctgcat cgcggcattc ctcgcgcttg gcatacaatc tgagcatagc aaacacgaag
      841 gatcgcaccg tggatcaaaa gcgcgtgctc atcaccggcg ccggcagcgg actcggccgc
      901 gcgctcgcct tctgctttgc cgaaaacggc tggcgcgtgg cctgcgcgga tatccgtctc
      961 gaccgcgcgc aggacaccgt gcgcctgatg cgcgatttcg gggtcggcgc gatggcactg
     1021 aaagtggatg tcggcagcga cgcttcggtg gaattcatgc acgacgaaat cctcgccgcc
     1081 tgggacggcg tcgacgtcgt cgtcaacaac gccggggtcg ccagcgcggg cagcgtcgcg
     1141 caaacctcgc tcgacgactg gcgctggacc ttgaacatcg atctgatggg cgtggtgcgc
     1201 gggtgccacg ccttcctgcc ggtgctgctc gaacaaggca gcggccacat cgtcaacatc
     1261 gcctcgttcg ccggcttcgc caacgcgccg cgcatgggcg cgtattcggc gacgaaggcc
     1321 ggcgtgattt cgctgtcgga aaccctgcgt gcggaactcg ctaacacggc cgtcaaggtg
     1381 tcggtcgcgt gtcccgcgtt cttcccgacc aatttgatgg acaccgcccg cgcgccggag
     1441 gccgacaagg caaccgcaaa aaaattcatg gccaaggcca cgcagagcgc cgacgatgtc
     1501 gccgccgtca tcttccgcga aatccagcgc ggcacgttcc tgatcctgcc gaccaagggt
     1561 gagcgcggac gctggcgcat caagcgtatc gcgcctgagc tgtttttccg caagctcgtg
     1621 gccctggctg gcgcgacgcg gatcaagcca tgagccgcct gctgatctac ggcgcgaacg
     1681 gctacaccgg ccgcctgatc gcgcgtgaag ccgtgcagcg tggactcaag ccgatgc
//
LOCUS       DASRVO010000074         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_27954, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000074 DASRVO010000000
VERSION     DASRVO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27954"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFF05_00885"
     CDS             <1..769
                     /locus_tag="VFF05_00885"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006448471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZW50390.1"
                     /translation="DVVLPDPIPGKGEMLCQVSNFWFGKTAHLVPNHLTGKSVAAVLP
                     TGTDAALYTKRSVVTKRLKPVPVEAIARGYLIGSGWKDYQATGALCGIKLPAGLRQAE
                     KLPEPIFTPSTKAAAGQHDENVAFDAVVKTIGADTAEQVRDATLKIYAFAADYAAQRG
                     ILIADTKFEFGTDEAGKLYVMDEMLTPDSSRFWPADEYRVGISPPSYDKQFVRDYLET
                     LDWNKTAPGPKLPASVIEGTAAKYAEALKKLAGIVLD"
     gene            complement(791..>2051)
                     /locus_tag="VFF05_00890"
     CDS             complement(791..>2051)
                     /locus_tag="VFF05_00890"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HZW50391.1"
                     /translation="AKKAPGLDWPEYFKAAGLDQVKQIDAWQAKAITGLSALTAHEPI
                     DSWKDLLVFHTLDDAAPLLPKAFADLQFDFYGQTLQGTPQQRERWKRAVEATNTDLGD
                     AVGQLYVAKYFPASSKTEVQALVKNLVAAFDKGIDNLAWMAPETRAKAKEKLVTLHVG
                     VGYPETWRNYATLDIKADDALGNQLRAAAFEYAYQKAKIGTPIDRGEWWMTPQTVNAV
                     NLPLQNALNFPAAILQPPFFDPKADAAANYGAIGAIIGHEISHSFDNTGSEFDAQGRL
                     ANWWTPADAAHFTTATDALAKQFDGYEALPGLHVSGKQTLGEDIADVSGLTAAYAAYH
                     LSLDGKPAPAIDDLTGDQRFFLAFGQDWRSKIRDAALRQRIATDVHAPAQFRAQTVRN
                     IDAWYAAFDVTLGEKLYLAPDQRVKIW"
BASE COUNT          351 a          648 c          673 g          379 t
ORIGIN      
        1 cgacgtggtg ctgcccgatc cgattcccgg caagggcgag atgctgtgcc aggtttccaa
       61 cttctggttc ggcaagactg cgcacctggt gccgaatcac ctgaccggca aatccgtcgc
      121 cgccgtgctg cccaccggca cggatgcggc gttgtataca aagcgcagcg tggtgacgaa
      181 acgcctcaag ccggttccgg tcgaggcgat cgcgcgcggt tacctgatcg gctccggttg
      241 gaaggattac caggcgacgg gtgcgctgtg cggcatcaag cttcctgcgg gcttgcgcca
      301 agcggaaaaa ttaccggagc cgatcttcac cccgtcgacc aaggccgcag ccggacagca
      361 cgacgagaat gtcgcgttcg atgcggtggt gaaaaccatc ggcgcggaca cggccgagca
      421 ggtgcgcgac gcgacgctga aaatctacgc gttcgccgcc gattacgccg cgcagcgcgg
      481 catcctcatc gccgacacca agttcgagtt cggcaccgac gaagccggca agctctacgt
      541 catggacgag atgctcacgc ccgattcctc gcgcttctgg ccggccgacg aataccgcgt
      601 cggcatcagt ccgccgagct acgacaagca gttcgtgcgc gattacctgg aaacgctgga
      661 ctggaacaag accgcacccg gtccgaagct gcccgccagc gtgatcgagg gcaccgcggc
      721 gaaatatgcc gaggccttga agaaactggc cggcatcgtg ctcgactgag cacgatgccg
      781 gtgcaagcgc ctaccagatc ttcacgcgct gatccggcgc gagatagagc ttctcgccca
      841 gggtgacgtc gaacgccgcg taccacgcat cgatgttgcg cacggtctgc gcgcggaact
      901 gcgccggcgc gtgcacgtcc gtggcgatgc gctggcgcag cgcggcgtcg cgaatcttgc
      961 ttcgccaatc ctggccgaag gcaaggaaga aacgctggtc gccggtgaga tcgtcgatcg
     1021 cgggtgcggg cttgccgtcc agcgacaggt ggtaggccgc gtaggccgcc gtcaggccag
     1081 acacgtcggc gatgtcctcg ccgagcgtct gcttgccgct cacgtgcagg ccgggcagtg
     1141 cctcatagcc gtcgaactgt ttggccagcg cgtcggtcgc ggttgtgaaa tgcgccgcgt
     1201 cggcgggcgt ccaccagttg gcgaggcgtc cttgcgcatc gaactcggaa ccggtgttgt
     1261 cgaagctgtg gctgatctca tggccgatga tcgcgccgat cgcgccgtag ttcgcggccg
     1321 catcggcttt tggatcgaag aacggcggtt gcaggatcgc ggcggggaag ttcagcgcgt
     1381 tctgcagcgg caggttcacc gcgttcacgg tttgcggcgt catccaccat tcgccgcgat
     1441 cgatgggcgt accaatcttc gccttctggt aggcgtactc gaacgcggcc gcgcgaagct
     1501 ggttgccgag cgcatcgtcc gccttgatat cgagcgtggc gtaattgcgc caggtttccg
     1561 gataaccgac gccaacgtgc agcgtcacga gtttttcctt ggccttggca cgagtttccg
     1621 gcgccatcca ggcgagattg tcgatgccct tgtcgaacgc ggcgacgagg ttcttcacca
     1681 gcgcctgcac ttcggtcttg gacgaggcgg ggaaatattt cgcgacgtac aactggccga
     1741 ccgcgtcgcc gaggtccgtg ttcgtcgctt ccaccgcacg tttccagcgc tcgcgctgct
     1801 gcggcgtgcc ctgcaaggtc tggccgtaga aatcgaattg caggtcggcg aaggcttttg
     1861 gcaacaacgg cgcggcgtca tcgagggtgt ggaagacgag caggtctttc caactgtcga
     1921 tcggctcgtg cgcggtcagc gccgacaatc ccgtgatcgc cttggcctgc caggcgtcga
     1981 tctgcttgac ctggtcgagt ccagcggcct tgaagtattc cggccagtcc agtcccggcg
     2041 ccttcttggc a
//
LOCUS       DASRVO010000075         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_157823, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000075 DASRVO010000000
VERSION     DASRVO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157823"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VFF05_00895"
     CDS             complement(<1..351)
                     /locus_tag="VFF05_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HZW50392.1"
                     /translation="MSGKARVSAITLHRASHILEVGFDSGETFRLPCEYLRVHSPSAE
                     VQGHSPSQRILQHGKRDVNIAAIEPVGHYGVLLRFNDGHDTGIFSWDVLHDLGVNHDA
                     NWQAYLDALAAAGLS"
     gene            complement(348..977)
                     /locus_tag="VFF05_00900"
     CDS             complement(348..977)
                     /locus_tag="VFF05_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50393.1"
                     /translation="MASIKHHREGNVVQFRGRGTRAQLREALESRVPVQIARSQVSPG
                     WVHGFVVGLAAEFCLIAEVSDAMQFDGFLALAVGDIDAVEQDPGKEFVEKALQLNDEQ
                     IPQLTDFKLDDWQAIAESAARLTPMLSLNMLDDENGEVSYIGRLTGAESDALILQEVD
                     PNAVWYPDTGAYEFPGIGSIGFGTQYMLLLAKIAGMPPVPLPPEALPAS"
     gene            complement(1006..>1503)
                     /locus_tag="VFF05_00905"
     CDS             complement(1006..>1503)
                     /locus_tag="VFF05_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZW50394.1"
                     /translation="RDLLPLVEGSTVSTKHGMVKTDHILFIASGAFSLAKPSDLIPEL
                     QGRFPIRVELSALSAGDFERILTEPDHALTKQYVALMATEGVTLDFTADGVRRMAEIA
                     FAVNERTENIGARRLHTVLERLLDTISYEAPDQDGKKYTIDAAYVDGHLAALVQDQDL
                     SRYIL"
BASE COUNT          273 a          467 c          482 g          281 t
ORIGIN      
        1 cgacagtccc gctgcagcca gcgcatccag atacgcctgc cagttggcgt cgtgattgac
       61 gcccagatcg tgcagcacgt cccaggaaaa gatgccggtg tcgtggccgt cattgaaacg
      121 cagcagcacg ccgtagtggc cgactggctc gatcgcggcg atattcacgt cgcgcttgcc
      181 gtgctgcagg atgcgttggc tcggactatg cccctgcacc tcggcgctgg gcgaatgcac
      241 gcgcaggtat tcgcaaggca ggcgaaacgt ctcgccgctg tcgaagccga cttcgaggat
      301 atgcgaggcg cgatgcaggg tgattgcgct gacgcgcgcc ttgccactca cgaggccggc
      361 agcgcttcgg gcggcaatgg caccggcggc atgccggcga tcttcgccag cagcagcatg
      421 tactgcgtgc cgaagccgat cgagccgatg ccggggaatt cgtaggcgcc ggtgtccgga
      481 taccaaaccg cgttcggatc gacttcctgc aggatcagcg catcggattc ggcgccggta
      541 aggcggccga tgtagctgac ctcgccgttt tcatcgtcga gcatgttgag cgagagcatc
      601 ggcgtaaggc gcgcggcgga ctcggcgatg gcctgccagt cgtcgagctt gaaatccgtc
      661 aactgcggaa tctgctcgtc gttgagttgc agcgccttct caacgaattc cttgccggga
      721 tcctgctcga ccgcgtcgat gtcgccgacc gcaagcgcga gaaagccgtc gaattgcatg
      781 gcgtcgctga cttccgcgat caggcagaat tcggcggcaa ggccgacgac gaaaccgtgc
      841 acccaacccg gcgagacttg cgaacgcgcg atctgcaccg gcacacggct ttccagcgcc
      901 tcgcgcagtt gtgcgcgtgt gccgcgtccg cggaactgga cgacattgcc ttcgcggtgg
      961 tgtttgatcg atgccatggt gcgaattgta ccggttcgcg ccgcgttaca gaatgtagcg
     1021 cgacaaatcc tgatcctgca ccagcgccgc cagatggccg tcgacatagg ctgcgtcgat
     1081 cgtatacttt ttcccgtcct ggtccggcgc ctcgtaggaa atcgtgtcaa gcaggcgttc
     1141 gagcaccgtg tgcaggcggc gcgcgccgat gttctcggta cgctcattta ccgcaaacgc
     1201 tatttcggcc atgcgccgca cgccgtcggc ggtgaaatcc agcgtcacgc cctcggtcgc
     1261 catcaatgcg acgtattgct tggtcagcgc gtggtcgggc tcggtgagga tgcgctcgaa
     1321 atcgcccgcc gacagcgcgg acaattccac gcggatcggg aaacgcccct gcaattccgg
     1381 aatcagatcc gatggcttgg ccaacgagaa tgcacccgaa gcgatgaaaa gaatgtggtc
     1441 ggtctttacc atgccgtgct tggtcgagac ggtcgagcct tcgaccagcg gcagcaggtc
     1501 gcg
//
LOCUS       DASRVO010000076         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_157900, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000076 DASRVO010000000
VERSION     DASRVO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157900"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFF05_00910"
     CDS             <1..586
                     /locus_tag="VFF05_00910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004086603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZW50395.1"
                     /translation="RIDQQHLRNGDLAEEDWPRVTSAITLLSETKILIDDTPALSPSE
                     LRARARRMKRQYDLGLIVVDYLQLMAVPGNKENRATEISEISRGLKALAKELNIPVIA
                     LSQLNRSLEQRTDKRPVMADLRESGAIEQDADVILFIYRDEYYDKESADKGLAEIIIG
                     KQRNGPTGTVKLTFLGRYTRFENTTAEARGTRYE"
     gene            583..1680
                     /gene="alr"
                     /locus_tag="VFF05_00915"
     CDS             583..1680
                     /gene="alr"
                     /locus_tag="VFF05_00915"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZW50396.1"
                     /translation="MSRAAVATIHLGALRRNLARVRERAGNAKVMAVVKADGYGHGLE
                     RVARALRDADAFGVASLGDGLRLRAAGIAKCVVVLSGPDEAADLAEFRRLGLDAVIHH
                     ESQLRWLEADRDARPLRAWLKIDTGMHRLGFAPDVARVAHARLRALANVDPDIVLMTH
                     FAASDEFDSPATPAQIAAFDAAVRDLPGAHSLANSAGLLGWPQVRGADSFKDWMRVGG
                     LLYGLSVVEGKTGADLGFEPAMTLSAKLIAVNRVGKGERVGYGGVYACPEDMPIGVAA
                     IGYGDGYPRSAPSGTPVLVNGVRAAVVGRVSMDLIAIDLRNAPDAQIGDRVTLWGPQL
                     PVEIVAEKSGTISYDLTCGMTKRVLFVEDTD"
     gene            1680..>2163
                     /locus_tag="VFF05_00920"
     CDS             1680..>2163
                     /locus_tag="VFF05_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003484290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZW50397.1"
                     /translation="MAKSKTAYVCNQCGAEYTKWQGQCESCGAWNTLSEFVVEPAKAA
                     GAVKRTGYAGAAPARIVPLTQVAAEAEVRTQIGIGELDRVLGGGLVQGSVVLVGGDPG
                     IGKSTLLLQVLGALGARLKSLYVSGEESLAQIAARGQRLSIALEPLHALAETCIERIL
                     E"
BASE COUNT          373 a          695 c          728 g          367 t
ORIGIN      
        1 ccgcatcgac cagcagcacc tgcgcaatgg cgatttggcc gaggaagact ggccgcgcgt
       61 gacgagtgcg atcaccttgt tgtccgagac gaaaatcctg atcgacgaca cgccggcgtt
      121 gtcgccgtcg gagttgcgtg cgcgcgcgcg gcgcatgaag cgccagtacg acctcggcct
      181 gatcgtcgtc gactacctgc agctcatggc cgtgcccgga aacaaggaga accgcgccac
      241 ggaaatttca gaaatctcgc gtggactgaa ggccctggcc aaggaactga acattcccgt
      301 gatcgcgctc tcgcagctca accgttcgct ggaacaacgc accgacaagc gcccggtgat
      361 ggccgacttg cgcgaatccg gcgcgatcga acaagacgcc gacgtgatcc tgttcatcta
      421 ccgcgacgag tactacgaca aggaatcggc ggacaagggc ctggccgaaa tcatcatcgg
      481 caagcagcgc aacggtccga ccggaacggt caagctcaca tttcttggcc gctacacgcg
      541 cttcgagaac accaccgccg aggcgcgtgg cacgcgatac gagtgagccg cgccgccgtc
      601 gccacgatcc atctcggcgc cttgcgccgc aacctcgcgc gcgtgcgcga gcgcgcaggc
      661 aacgcgaagg tgatggcagt ggtcaaggcc gatggctatg gccacggctt ggagcgcgtc
      721 gcgcgtgcgt tgagagatgc cgacgcattc ggcgtcgctt cgctgggcga tggcttgcgc
      781 ctgcgtgcgg ccggcatagc caagtgcgtc gtcgtgttgt ccgggcctga cgaagcggcg
      841 gacctcgccg aattccgccg gcttggcctg gatgccgtca tccatcacga atcgcaactg
      901 cgctggctcg aagccgaccg cgacgcacgc ccgttgcgcg catggctaaa gatcgacacc
      961 ggcatgcatc gccttggttt cgcaccggat gtcgcgcgcg tcgcgcacgc gcgactgcgc
     1021 gcgctcgcga acgttgatcc cgacatcgtg ctgatgacgc acttcgccgc gtccgacgaa
     1081 ttcgattcgc cagcgacgcc tgcgcagatt gccgcgttcg acgcagccgt gcgcgacctg
     1141 cccggcgcgc attccctggc caattccgcc ggcttgcttg gctggccgca ggtgcgtggc
     1201 gccgattcat tcaaggattg gatgcgtgtg ggtggcttgt tgtatggcct gtccgtggtc
     1261 gaaggcaaga ccggcgccga tctcggcttc gagccggcga tgaccttgtc cgcgaaactc
     1321 atcgcggtca atcgcgtggg caagggtgag cgcgtcggtt acgggggcgt ctacgcttgt
     1381 cccgaagaca tgcccatcgg cgtcgccgcc atcggttatg gcgacggcta tccgcgcagc
     1441 gcgccgtcgg gaacgccagt gctggtgaat ggcgtgcgtg ccgcggttgt gggtcgcgtg
     1501 tcgatggact tgatcgccat cgatttgcgc aacgcgcccg acgcgcaaat cggcgaccgc
     1561 gtgacgctgt ggggtccgca actgcccgtc gagatcgttg ccgaaaaatc cggcacgatt
     1621 tcctacgacc tcacctgcgg catgaccaag cgcgtgttgt tcgtggagga tacggactga
     1681 tggcaaagtc caagaccgcc tacgtctgca accagtgcgg cgccgagtac acgaagtggc
     1741 agggccagtg cgaatcctgc ggggcgtgga acacgctgag cgaattcgtg gtcgagccgg
     1801 cgaaggctgc cggcgcggtc aagcgcaccg gttacgcggg tgcggccccg gcgcgcatcg
     1861 tgccgctgac gcaggtcgcg gccgaagccg aggtgcgcac gcaaatcggc atcggcgagc
     1921 tggaccgcgt gctcggcggc gggctggtgc agggttcggt ggtgctggtc ggcggcgatc
     1981 ccggcatcgg caaatccacg ctgctcttgc aggtgctcgg cgcactcggc gcgcgcctga
     2041 aaagcctgta tgtcagcggt gaggaatccc ttgcgcagat cgccgcgcgc ggccagcgcc
     2101 tgagcatcgc tctggaaccg ctgcatgcgc tggccgaaac ctgcatcgag cgcattcttg
     2161 aga
//
LOCUS       DASRVO010000077         1347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_261933, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000077 DASRVO010000000
VERSION     DASRVO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261933"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFF05_00925"
     CDS             <1..930
                     /locus_tag="VFF05_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW50398.1"
                     /translation="ISASLGLALLGGENASVDSVVTNAQAAQLAAAHMGGNRVLWFEA
                     KEAALLPIDPLLAVRAVLSRPLVPEQTQYEFAAIAPLAGKLHGQFELGFKVRSVQHPG
                     TSVSYEELAPVAAECNQIATLDRLLLQHSLEVREEQLRRGRQLRLFVPQAVSSAMESE
                     FAWWLERQLKSRHLSGTGLTIELACSTLIDAGERAREQVKALHQYGVRFCLVDYGRDW
                     AAVHALKSLNVDFVRLAPSLVEELGGAKSVSDTMLALVRKAHASGVAVIAPEVDSLQR
                     AHLLLRLGVDYGIGPAFARPQPQPDFDFNRPLW"
     gene            complement(1057..>1347)
                     /locus_tag="VFF05_00930"
     CDS             complement(1057..>1347)
                     /locus_tag="VFF05_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HZW50399.1"
                     /translation="EQLRTCFDPEIPVNIVELGLVYACDVGHGEDGKRKVEVKLTLTA
                     PGCGMGDILVDDVRSKLMAIPTIAEADVELTFEPPWNHTMMSDVAKLETGML"
BASE COUNT          234 a          443 c          438 g          232 t
ORIGIN      
        1 atcagcgcaa gcctcggcct ggccctgctc ggcggcgaga acgccagcgt ggattcggta
       61 gtcaccaacg cacaggcggc gcaactggcc gccgcgcaca tgggcggcaa ccgcgtgctg
      121 tggttcgagg ccaaggaagc cgcgctacta ccgatcgatc cgctgctcgc tgtgcgcgcg
      181 gtgctcagcc gaccgctggt gcccgagcag acgcaatatg aattcgccgc gatcgcaccg
      241 ctcgccggca agctgcacgg ccagttcgaa cttggtttca aggtgcgcag cgtgcagcat
      301 cccggcacca gtgtgtccta cgaggaactc gcaccggtcg ccgccgagtg caatcagatc
      361 gccacgctcg atcggctgct gctgcaacat tcgctggaag tgcgcgaaga acaactgcgc
      421 cgcggccgcc agttgcgcct gttcgtgccg caagcggtat ccagcgcgat ggagtcggaa
      481 ttcgcgtggt ggctggagcg ccaactcaag tcgcggcacc tgtccggcac cggcctgacc
      541 atcgagctgg cctgctcgac cctgatcgac gccggcgaac gcgcgcgcga gcaggtcaag
      601 gccctgcacc agtacggcgt gcgcttctgc ctcgtcgact acggccgcga ctgggccgcg
      661 gtgcacgcgc tgaagagcct gaacgtggat ttcgtgcgcc tggcgccttc gctggtcgaa
      721 gaactgggcg gcgcaaaatc ggtcagcgac accatgctcg cgctggtgcg caaggcgcat
      781 gcgagcggcg tggcggtgat cgcgccggaa gtcgacagcc tgcagcgcgc ccacctgctg
      841 ctgcgcctgg gcgtggacta cggcatcggc ccggcgtttg cgcggccgca accgcagccg
      901 gatttcgatt tcaaccgtcc gctgtggtag aaaccggagc gcctacgcga tgctttgaat
      961 ggcatcgcgt gcgacggttt ctggtgaggc atggtcgaac atcgcgccgg cgatgtgagg
     1021 gaacggcagc gcacaaggat ggttgcggcg atgcacttac aacatcccgg tttccagctt
     1081 cgccacatcc gacatcatcg tgtgattcca cggcggctcg aaggtgagtt ccacgtccgc
     1141 ctcggcgatg gtcggaatcg ccatgagctt gctgcgcacg tcgtcgacaa ggatgtcgcc
     1201 catgccgcag ccgggcgcgg tcaacgtcag tttcacttcg accttgcgct tgccgtcctc
     1261 gccatgcccg acatcgcacg catagaccag acccaactcg acgatgttga ccggaatttc
     1321 cggatcgaaa caggtgcgca gttgttc
//
LOCUS       DASRVO010000078         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_157940, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000078 DASRVO010000000
VERSION     DASRVO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157940"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFF05_00935"
     CDS             complement(<1..962)
                     /locus_tag="VFF05_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZW50400.1"
                     /translation="MARGVTEADAAHRFLHPSLDHLHSPYAMLGMKAAVERLRAAIEL
                     REPILIYGDYDVDGTTAVVILKTAIELCGGTADFHVPHRIKEGYGIKADVIEQAAAAG
                     IRLVISVDTGIRAFAAADAAHRLGLDLIVTDHHLPETHSGMPRALAVLNPHQPGCDYP
                     CKELCGAGVAFKIAQALMENLGRPQLVPSFLKMVAIATIADAVPLVGENRVFAALGLE
                     GLRRPVNVGLKALFQAAAIGSARPLTTGDVAFKIAPRLNAAGRMDVASDVVDLFSTRD
                     AEQAALIATRLNQLNSDRQQEELRIVEAIDELMRTSPEMHDAYCL"
BASE COUNT          178 a          319 c          349 g          170 t
ORIGIN      
        1 agacagtacg catcgtgcat ctcgggagaa gtgcgcatta gttcgtcaat ggcttcgacg
       61 atgcgcagct cttcctgctg gcggtcggag ttgagctggt tgagccgcgt ggcgatgagc
      121 gccgcctgct ccgcatcgcg cgtgctgaac aggtccacca cgtcgctggc cacgtccatg
      181 cgcccggcag cgttaaggcg cggcgcgatc ttgaaggcga cgtcgccggt agtgagcggg
      241 cgcgcgctgc cgatcgccgc cgcttggaac agcgccttca gcccaacatt caccggacgg
      301 cgtaatcctt cgaggccaag agcggcgaag acgcggttct cgcccaccag cggcaccgcg
      361 tcggcaatgg tggcgatcgc caccatcttc agaaacgacg gcaccaattg cggccgcccg
      421 agattttcca tcagcgcctg cgcaatcttg aatgcgactc cggcgccgca caattccttg
      481 cagggataat cgcaaccggg ctggtgcggg ttgagcaccg ccagcgcgcg cggcatcccg
      541 gagtgagttt cgggcaggtg atggtcggtc acgatcaggt caaggcccag gcggtgcgcg
      601 gcgtcggcgg cggcgaaggc tcggatgccg gtgtccacgc tgatgaccag gcgaatgccg
      661 gcggcggcgg cctgctcgat cacgtccgcc ttgatgccgt aaccctcctt gatgcggtgc
      721 ggtacgtgaa agtcggccgt gccgccgcac agttcaatgg cggtcttgag aatgaccacg
      781 gcagtggtgc cgtccacatc gtagtcgcca taaatgagga tgggctcgcg gagttcgatg
      841 gcggcgcgca gacgctccac cgcggccttc atgcccagca tcgcataggg cgaatgcagg
      901 tgatcgagcg aaggatggag gaaacggtgt gccgcgtctg cctcagtaac gccgcgcgcc
      961 accagcaacc gcgccagcgt gggcgacagg ccggcttcct gggcgaggtg agtgac
//
LOCUS       DASRVO010000079         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_105846, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000079 DASRVO010000000
VERSION     DASRVO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105846"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFF05_00940"
     CDS             <1..239
                     /locus_tag="VFF05_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50401.1"
                     /translation="ERAANEAMRAAERKSMLARDGLGDPPTEAEEQEVKRLREIADAL
                     FRQATFALHLGTREVRTGQRLGYDEIWSLVHRST"
     gene            397..909
                     /locus_tag="VFF05_00945"
     CDS             397..909
                     /locus_tag="VFF05_00945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZW50402.1"
                     /translation="MHADELRSIQAPLKDRYSASPDAALITLRAQGRLGEGVNCKLET
                     GKGMVTAGLHPASGGNGLSACSGDMLLEALVACAGVTLNAVATALGIELRDAVVQAQG
                     DLDFRGTLGVAKEVPVGFQAIRLQFMLDSDASDEQLATLLRLTERYCVVYQTLVRPPT
                     LTATRQTLQA"
BASE COUNT          179 a          335 c          371 g          163 t
ORIGIN      
        1 ccgagcgcgc ggccaacgaa gcgatgcgtg cggcagagcg caagtcgatg cttgcgcggg
       61 atggccttgg cgatcctccc accgaggccg aggagcagga agtgaagcgg ttgcgcgaaa
      121 tcgccgacgc gctcttccgg caggcgacgt tcgcgctgca tctcggcacg cgcgaggtgc
      181 ggaccggcca gcgccttgga tatgacgaga tctggagctt ggtgcatcga tccacgtgag
      241 cgtctggacg cgacccacgg cgcgccgcgc aagttccctc gcctggaccg ttgtcaccag
      301 acggtgacct gagtcgcggt gcgcgttgac agcgcgccgc ggcaacggat aatccgggcc
      361 cccgcgcgat ccgaatcccg ggaaaggagt ttccccatgc atgccgatga gttgcgttcc
      421 atccaggccc cattgaaaga ccgatacagc gcgtcgcccg acgctgcact gatcaccttg
      481 cgggcgcagg gacgcctggg cgaaggcgtg aactgcaagc tggagacggg caaaggcatg
      541 gtgaccgccg gcttgcatcc ggcgtccggc ggcaatggac tgagcgcctg ctccggcgac
      601 atgctgctcg aagcgctggt cgcctgcgcg ggcgtcacgc tcaatgcggt ggcgacggcg
      661 ctggggatcg aactgcgcga tgccgtggtg caggcgcagg gcgatctgga ttttcgcggg
      721 acgctcggcg tcgccaagga agtgccagtg ggattccagg ccatccggct gcagttcatg
      781 ctggattccg acgcctcgga tgaacaactc gcgacactgc tgcgcctgac cgaacgctac
      841 tgcgttgtgt atcaaacgct ggttcggccg ccgacactca cggcaacgcg acagacgttg
      901 caggcctgag cgcggcgaac cggattgctc ctacccgacg atgccgctgg cgcgcagggc
      961 ggcgatctcg ctgtcggtgc ggcccaacaa gcttgcgagg acggcgtcgg tggaatcgcc
     1021 aagccgcggc ggcggccggt cgtagcgc
//
LOCUS       DASRVO010000080         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_157999, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000080 DASRVO010000000
VERSION     DASRVO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157999"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFF05_00950"
     CDS             complement(<1..267)
                     /locus_tag="VFF05_00950"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HZW50403.1"
                     /translation="MTSDLLFVYGTLMCGYHHPMAQLLSRSADFLGPARCRGRLYLVK
                     HYPGLVLSEDADDVTFGELYRLRQAGELLREFDMYEACGEGFAAP"
     gene            472..>1133
                     /locus_tag="VFF05_00955"
     CDS             472..>1133
                     /locus_tag="VFF05_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZW50404.1"
                     /translation="MLLDVEDVPPVLEHNTAGRSPFLLTSDHYGRAIPRGLGDLGLPQ
                     SEFERHIAWDAGIAGVANALSKQIDAHLIAQRYSRLVIDCNRPPNAASSIPRISEATT
                     IPGNEGISPSAAEARRAAIFDPYHHRIAEVIDRRLRDDIPTVLVALHSFTPVYAGVIR
                     PWHIGTLYHRDTRLPPLLLNHLRAEPDLVVGDNEPYAVSDETDYTIPVHGEKRGLMNS
                     GI"
BASE COUNT          226 a          373 c          340 g          194 t
ORIGIN      
        1 cggcgcggca aaaccttcgc cgcaggcctc gtacatgtcg aactcgcgca gcagttcgcc
       61 cgcctgacgc agccggtaca actcaccgaa cgtgacgtca tccgcgtcct cggacagcac
      121 cagccccgga taatgcttca ccagatagag ccggccgcgg cagcgcgccg gccccagaaa
      181 atcggcgctg cgcgacaaca gctgcgccat cggatgatga tagccgcaca tcagcgtgcc
      241 atagacgaag agaaggtcgg aggtcatggg aatgtctata gccgtcatca cgcgccgacg
      301 ggtcgcgcga atgcgcgccc gatgataaac tccgcgaagc gattcacgca atcgtcatga
      361 cttcgggcgg gccaatctat gccgatctct gccgatttct aaaatcggca ttctgcttcg
      421 cctggcaagg ggcaaggaaa gatggatttg gcaggccttc gcgacacatc attgctcctg
      481 gatgtcgagg acgttccgcc ggtgctcgag cacaataccg ccgggcggtc gccgttcctc
      541 ctgacctcgg accattacgg ccgggcgatc ccgcgcgggc tcggcgatct cgggctgccg
      601 caaagcgagt tcgaacggca tatcgcctgg gatgccggca ttgccggcgt cgccaatgcg
      661 ctatcgaaac agatcgacgc gcatctgatc gcgcagcgct attcgcggct ggtgatcgac
      721 tgcaatcgcc cgcccaacgc ggcaagctcg atcccgcgca tcagcgaggc aacgacgatt
      781 ccgggcaatg aagggatctc accatcggcg gccgaggcgc ggcgggcggc gatcttcgat
      841 ccctatcatc accgcatcgc cgaggtgatc gaccgccggc tgcgcgacga cattccgacg
      901 gtgctggtgg cgctgcacag tttcaccccg gtctatgccg gcgtcatcag gccctggcat
      961 atcggcacgc tgtatcaccg cgacaccaga ttgccgccgc tcttgttaaa tcatttgcgc
     1021 gccgaacccg atctcgtggt cggcgacaac gagccctatg cggtcagcga cgagaccgac
     1081 tacacgattc cggttcacgg cgagaagcgc ggcttgatga attcaggcat cga
//
LOCUS       DASRVO010000081         1257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_209733, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000081 DASRVO010000000
VERSION     DASRVO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209733"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..>1257)
                     /gene="galK"
                     /locus_tag="VFF05_00960"
     CDS             complement(208..>1257)
                     /gene="galK"
                     /locus_tag="VFF05_00960"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HZW50405.1"
                     /translation="DYNDGFVFPAAIDLFTWVGVAPRDDARIRVRSEDFNEAVEFEIG
                     ALPRDTDPAWSRYIAGVALILQRDGQRLNGANLLIRGEVPIGAGVSSSAALEVAAGFA
                     LLANSGVTMEGRKLARLCQQAENEIVGARCGIMDQLASACCAADRALLLDCRSLEYRM
                     LPLPAGVRLVICNTMVKRELTGSAYNTRRAECEEGVQILAKRFPAVRALRDATIEQLD
                     ECRAAMPENVFRRCRHVITEDARVLQAAEALEGNRLDDLRRAMAESHRSLREDYQVSC
                     AELDLLVELANRERGVHGARMTGAGFGGCTINLVDAECVDDFREHVSAGYEQRTRIKP
                     DIYVCHAADGVKRAE"
BASE COUNT          217 a          411 c          400 g          229 t
ORIGIN      
        1 gtatttcccg ggcacatgta gcacgtcgga tcgtacgcga cggccgtctc cggctgcacc
       61 gcttcgattt gcccctgcca ggggcgctgc gtgcggtgcg gcgaaacgag cacccactcg
      121 cacagcaagg gattgaatcg ccggtgaggt gtctccgcga aattgtgggg catcagatct
      181 ttcttgtctt tcttgcgcct cgaatcgtta ttcggcgcgc ttcacgccgt ccgcggcgtg
      241 gcagacataa atgtcgggct tgatgcgcgt gcgctgctcg tagccggccg aaacgtgctc
      301 gcggaaatca tcgacgcatt cggcgtcgac gaggttgatg gtgcatccgc caaaacctgc
      361 gccggtcatg cgcgcgccgt gcacgccgcg ttcacgattg gccagctcga ccagcagatc
      421 aagctcggcg cagctcacct ggtaatcttc gcgcaggctg cggtgcgatt cggccattgc
      481 gcggcgcaaa tcgtcgagcc ggttgccttc gagcgcttcg gccgcctgca gcacgcgcgc
      541 gtcttccgtg atgacgtggc ggcagcgtcg aaacacgttt tcgggcatgg cggcgcggca
      601 ctcgtcgagc tgctcgatcg tcgcatcgcg cagcgcacgc acggcaggaa agcgcttcgc
      661 gaggatttgc acgccctctt cgcactcggc gcggcgcgtg ttgtaagcgc tgccggtcag
      721 ctcgcgcttc accatggtat tgcagatcac cagacgcact cccgcgggca gcggcagcat
      781 gcgatattcg agcgagcggc aatcgagcag cagggctcga tcggcggcac agcaggccga
      841 ggccagctgg tccatgatgc cgcagcgcgc gcccacaatt tcgttttccg cttgctggca
      901 gaggcgcgcc agcttgcgtc cttccatggt gacgccggaa ttggccagca gcgcaaatcc
      961 cgccgccact tcgagcgccg ccgaagaact cacacccgcg ccgataggca cttcgccgcg
     1021 aatgagcagg ttcgcgccgt tcaggcgctg gccgtcgcgc tgcaggatca gcgcaacgcc
     1081 cgcaatgtag cggctccatg ccggatcggt gtcgcgcggc agcgcgccaa tttcgaattc
     1141 caccgcttca ttgaaatctt cggaacggac gcgaatgcgc gcgtcatcgc gcggcgcaac
     1201 gcctacccag gtgaacaaat cgatggcggc gggaaaaacg aagccgtcgt tgtaatc
//
LOCUS       DASRVO010000082         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_288035, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000082 DASRVO010000000
VERSION     DASRVO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288035"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..2572)
                     /locus_tag="VFF05_00965"
     CDS             complement(68..2572)
                     /locus_tag="VFF05_00965"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HZW50406.1"
                     /translation="MTANARRSIIALPLALAASLGFSAAHAAPAPTHPDKVFDATIAL
                     QGLLPVHVDKHAGRILVTLPPAGDDGVSARFLYATSLRTGLGAAPTFLDRGRVGNTQI
                     LAFRRYGKKIAVVFENPRFRGAATGADPSPDFATSIAWMGDVAATLPDGRLVVDLSGF
                     LTADAIGIADSLDQGTDTFGVGGGQGAGKGFKLDEKRSAADPDSVKDFPDNLEIDAVQ
                     TYASDTPGEEVANIAPATGQVSFTVHHSFVKLPGPGFHPRAFDPRIAGFGTQVVDFGE
                     PLGRDVVREYANRFRLEKTDPGAARSRVRKPIVYYVDWRAPEPIRSALIDGIGWWVKA
                     FDAAGYIDAFQVKLLPRDVDPMDVRYNVVNWVDRATRGWSYGQEIVDPRTGEIVKGMV
                     VIGSLRARQDIQIFEGLVGADKLGKGGLDDPVQAALARIRQLGAHEVGHTLGFAHNFA
                     ASTQDRTSVMDYPPPRIGLVDGHPDLSHAYASGVGQWDMATVDWLYGEPAPGQDAQAW
                     ADAQAKRIVDSGLRYIQDDDARADDTSQPWASLWDDGADATAELIHLMGVRRVAIDRF
                     GLSALAPGEPMANLRRRFVPVWLLHRYQMVAAAKAIGGVYLQYAVNGGGHEASDPVPP
                     KAQRAALDAVLATLAPDPLRVPQRLVPLLSAARNGSPNRQYGIELFAGSGGTPFDPLV
                     AADTAAELTLNALLAPTRLARVEMQHAAEADALGVQELLDRLLAATLPDKTDALSRRI
                     AWRTLMDIARAAHDPKTTPGVVALLDQRLHEEALALAQRTGDAAERAWGASLSRLLLD
                     PRELDKELARHARATPVPPGSPIGAEADWLSLPGDD"
     gene            complement(2678..3214)
                     /locus_tag="VFF05_00970"
     CDS             complement(2678..3214)
                     /locus_tag="VFF05_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HZW50407.1"
                     /translation="MRSRGCTLLATLFVVLAGNASAALPAPGQACMVQALQAMLAPSV
                     LDELSAADWLSRASAGTSDPPARDAEPRRLLVDFAMTLRDIRYRRGGRNPRSGFDCSG
                     FVHYVFAQVLGVDLPDNSAAQFRDSQKIDRDDLQAGDLVFFRTRGKRVSHVGIYLGGG
                     RFIHSPTTGERVRVDRLS"
     gene            3332..>3593
                     /locus_tag="VFF05_00975"
     CDS             3332..>3593
                     /locus_tag="VFF05_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZW50408.1"
                     /translation="MRICVYCASSQMCAAAYHAAATRLGEILAEAGCTIVYGGGAVGL
                     MGSLANGALARGGKVIGIIPRFMTDVEWQHPGIENLEVVEDMR"
BASE COUNT          603 a         1212 c         1218 g          560 t
ORIGIN      
        1 aaaccgtgcc gcgcatgacc gtcagggtca caatcgccgc gccgcggaca atgccgcagc
       61 gccgggatca atcgtctccc ggcagcgaca gccaatccgc ctcggcaccg atcggcgaac
      121 cgggcggcac cggcgtcgct cgcgcatggc gggcaagctc cttgtccagc tcgcgcggat
      181 cgagcagtaa ccgcgacaga ctggcgcccc atgcacgttc ggccgcgtcg cccgtgcgtt
      241 gcgccagcgc aagcgcctcc tcgtgcaggc gctggtcgag cagcgcgacc acgccggggg
      301 tggtcttggg gtcgtgcgcg gccctcgcga tatccatcag ggtgcgccag gcgatgcgcc
      361 gggacagcgc gtcggtcttg tccggcagcg tcgccgcgag caggcgatca agcagttcct
      421 gcacacccaa ggcatccgcc tctgctgcgt gttgcatctc cacacgcgcc agtcgcgtgg
      481 gggcgagcaa tgcgttcaag gtcagttcgg cggcggtgtc ggcggcgacc agcggatcga
      541 acggcgtgcc gcccgaaccg gcgaacaact cgatgccgta ctgccggttc gggctgccgt
      601 tgcgcgcggc cgagagcaac ggcaccagcc gttgcggaac gcgcagcggg tccggcgcca
      661 gcgtggcgag gacggcatcg agcgcggcgc gctgtgcctt tggcggtacg ggatcggacg
      721 cctcgtggcc gccgccgttg acggcgtatt gcaggtacac gccgccgatc gccttcgcgg
      781 cagcgaccat ctgataacgg tgcaggagcc acacgggcac gaagcgccga cgcaaattgg
      841 ccatcggctc gcccggcgcc agcgccgaca ggccgaagcg atcaatcgcg acgcgccgca
      901 cgcccatcag gtgaatgagt tcggcagtcg cgtccgcacc atcgtcccac agcgaagccc
      961 atggttgtga ggtatcgtcg gcgcgcgcgt cgtcgtcctg gatgtagcgc aggccggaat
     1021 cgacgatccg ttttgcctgc gcatcggccc aggcctgcgc gtcctgtccc ggcgccggct
     1081 cgccgtacag ccagtcaacc gtggccatgt cccactggcc gacgccactc gcataggcat
     1141 ggctcaggtc cggatgcccg tcgaccaggc cgatccgcgg cggcggataa tccatcaccg
     1201 aggtgcgatc ctgtgtgctc gcggcgaaat tgtgcgcgaa gccgagcgtg tgtccgactt
     1261 cgtgcgcgcc gagctggcga atgcgcgcca gcgccgcctg caccggatcg tcgagaccgc
     1321 ccttgccgag cttgtcggcg ccgaccaggc cttcgaagat ctggatgtcc tggcgcgcgc
     1381 ggagcgagcc gatcaccacc atgcccttga cgatttcgcc ggtgcgcgga tcgacgatct
     1441 cctgcccgta ggaccagccg cgcgtggcgc gatcgaccca gttgacgacg ttgtaacgca
     1501 cgtccatcgg atcaacatcc cgcggcagca gcttgacctg gaaggcgtcg atgtagccgg
     1561 ccgcatcgaa cgccttgacc caccagccga ttccgtcgat caatgccgac cgaatcggtt
     1621 ccggcgcgcg ccagtcgacg tagtagacga tgggcttgcg cacgcgcgag cgcgccgcgc
     1681 ccggatcggt cttttccagg cggaaccggt tggcatattc gcgcaccacg tcgcggccca
     1741 gaggttcgcc gaagtccacg acctgcgtgc cgaagcccgc gatgcgcgga tcgaacgcgc
     1801 gcgggtggaa tccgggaccg ggcagcttca cgaaactgtg gtgcacggtg aaactgacct
     1861 gcccggtggc gggcgcgatg ttggcgactt cctcgcccgg cgtgtccgac gcgtaggtct
     1921 gcaccgcgtc gatttcgagg ttgtccggaa aatccttcac cgaatcggga tcggccgcgc
     1981 tgcgcttctc gtcgagcttg aagcccttgc cggcgccctg cccgccgccg acgccaaacg
     2041 tgtccgtgcc ctggtcgagc gagtcggcga tgccgatcgc atccgcggtg aggaatccgg
     2101 acaggtccac caccaggcgg ccatcgggca gggtcgcggc aacgtcgccc atccatgcga
     2161 tcgacgtggc aaagtcggga ctggggtcag cgccggttgc ggcgccgcgg aagcgcggat
     2221 tctcgaacac cacggcgatc ttcttgccgt agcggcggaa cgccaggatt tgcgtattgc
     2281 cgacccggcc gcggtccagg aacgtcggcg ccgcaccgag gccggtacgc agcgaagtgg
     2341 cgtacaggaa tcgcgccgac acgccatcgt cgccggccgg cggcaaggtg acgagaatgc
     2401 ggccggcgtg cttgtccaca tgcaccggca gcaggccttg cagcgcgatc gttgcgtcga
     2461 aaaccttgtc cggatgcgtt ggcgccggcg cggcgtgcgc ggcggaaaat cctagggatg
     2521 ccgcgagagc gagcggcagt gcgattatcg aacggcgggc gttggcagtc atgcgcagga
     2581 ttctatcccg cgcggaagct gggcgctcag aaccgcaccc tgctcagatc aaggcatccg
     2641 gccgtcgcgc gccggcaaac cggtgcgccc agtattgcta gctgaggcga tccacgcgca
     2701 cgcgctcgcc cgtcgtgggc gaatggatga agcgtccgcc accaaggtaa atgccgacat
     2761 gcgagacgcg tttgccgcgg gtgcggaaga acaccaggtc accggcttgc aagtcgtcgc
     2821 ggtcgatttt ctggctgtcg cggaattgcg cggcggagtt atccggcaag tccacgccca
     2881 gcacctgggc gaagacgtaa tgcacgaacc cgctgcaatc gaagccgctg cgcgggttgc
     2941 gcccgccgcg gcgatagcgg atgtcgcgca gggtcattgc aaaatcgact agcaagcggc
     3001 gcggttcggc atcgcgggcg ggcggatccg acgtgcccgc agacgcgcgc gacagccagt
     3061 cggcggccga cagttcatcc agcacggacg gtgcgagcat cgcctgaagc gcctgcacca
     3121 tgcacgcttg ccccggagca ggcagtgcgg ccgacgcgtt gccggccagc acgacgaaca
     3181 aggtggccag caatgtacaa ccccgactgc gcatgcccac aaactccggt gtatctgccc
     3241 cggcagacaa cgaagcggcg acgctagcag aggattcatt cgattcccgt taattccgtt
     3301 agacttcgcc ggtcgttttt ttggagttcc catgcgaatt tgcgtctact gcgcctccag
     3361 ccagatgtgc gccgcggcct atcacgccgc ggccacgcga ctcggcgaaa tccttgccga
     3421 agccggatgc accatcgtct acggtggcgg cgctgtcggc ctgatgggtt cgctggccaa
     3481 tggcgcgctc gcccggggcg gcaaggtgat cggcatcatt ccgcgcttca tgaccgacgt
     3541 ggaatggcag catccgggca tcgagaatct cgaagtcgtc gaggacatgc gcg
//
LOCUS       DASRVO010000083         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_209787, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000083 DASRVO010000000
VERSION     DASRVO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209787"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VFF05_00980"
     CDS             complement(<1..1067)
                     /locus_tag="VFF05_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZW50409.1"
                     /translation="MSESNDNLPSDENKLIAERREKLHALRAHGIAFPNDFHPDAFAG
                     DLQAEFEGRDAAAVEAVARRVGVAGRLLAKRIMGKASFVQIQDMTGRIQLFLQHGALA
                     ETYDAFKSWDVGDIVGAEGVLMRTKTGELSVKVDKLRLLTKSLRPLPDKWHGLADVEQ
                     RYRQRYVDLIITPEARRVFVLRSAIIRFIRQWLEAAPRRFMEVETPMMHVIPGGATAR
                     PFVTHHNALDMDLYLRVAPELYLKRLTVGGFDRVYEINRNFRNEGVSTRHNPEFTMLE
                     LYQAYATYNEIMDLTEAMIRATAQSVAGGTLLPWDGSRIDVGPPFRRWRMEDAVLEHN
                     PEIRREELRDRSALAAHCARLR"
BASE COUNT          191 a          389 c          398 g          214 t
ORIGIN      
        1 cgcaggcgcg cgcagtgcgc ggcaagcgcg ctgcggtcgc gcagctcctc gcgacgtatc
       61 tccgggttgt gctcgagcac ggcgtcttcc atgcgccagc gtcggaacgg cggcccgacg
      121 tcgatccggc tgccgtccca cggcagcagc gtcccgcccg cgaccgattg cgccgttgcc
      181 cggatcatcg cctcggtcag gtccatgatc tcgttgtagg tcgcataggc ctgatagagc
      241 tcgagcatcg tgaactcggg attgtgccgg gtcgagacgc cctcgttgcg gaagttgcgg
      301 tttatctcgt agacccggtc gaaaccgccg acggtcaggc gcttgaggta cagttccggc
      361 gcgacgcgca ggtaaaggtc catgtcgagc gcgttgtgat gggtgacgaa cggacgcgcg
      421 gtcgcgccgc ccggaatcac gtgcatcatc ggcgtctcga cttccatgaa ccggcgcggc
      481 gcggcctcga gccactggcg gatgaagcgg atgatcgccg agcgcagcac gaacacccgc
      541 cgtgcttccg gcgtgatgat caggtcgacg tagcgctggc ggtagcgctg ctcgacgtcc
      601 gccagcccgt gccacttgtc cggcagcggc cgcagcgact tggtcagcag gcgcaatttg
      661 tcgactttta ccgaaagctc gccggtcttc gtgcgcatca gcacaccttc ggcgccgaca
      721 atgtcgccga cgtcccagga cttgaacgcg tcgtaggttt cggccagcgc gccatgctga
      781 aggaacagct ggatgcggcc ggtcatgtcc tggatctgga cgaagctggc cttgcccatg
      841 atgcgcttcg ccagcagccg cccggcgacg ccgacccggc gcgccaccgc ctctacggcg
      901 gccgcgtccc tgccttcgaa ctcggcctgg aggtcgccgg cgaacgcgtc cggatgaaaa
      961 tcgttcggaa acgcgatgcc gtgcgcacgc agtgcgtgca gcttctcgcg acgctcggcg
     1021 atcagcttgt tttcgtcgga gggcaggttg tcgttcgatt cgctcatggg atcgttcggc
     1081 gggaagtcga ggcgcggcaa ttgaaacagg cgtcattccc gcgcaagcgg gaatccattt
     1141 tgactgtcgg cccgcggaga aggatcaaca tcgatccccg cttgcacggc ga
//
LOCUS       DASRVO010000084         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_80088, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000084 DASRVO010000000
VERSION     DASRVO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80088"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1677
                     /locus_tag="VFF05_00985"
     CDS             <1..>1677
                     /locus_tag="VFF05_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZW50410.1"
                     /translation="KSANPGLGEVDYGLTEAQSVVYVDATYSGQSFGTSLAFPFSNPN
                     GACAATTAYFGINAFATIADGLMHVTNAGTVCIAKSNYAEAVNITNNVQMIGDGNTAG
                     DTVIAGAVTLNAGGIVGAPLLMQNLRVTNASGKGVAVGAASHLAFYGVAFADNGDAGL
                     DFDSVIDDVAISGSLFDANTGAGIRTATTAQVSNVTINGGTVFSNNAAGIILFGASGS
                     GNGQISNWTIDSSQFLSNDNADASNFGGAIWLKTGGAGSAINGFTVTNSIFADNGSSN
                     TLNQVGITVRARPGTTMTGVNICNNTFEETAAPGTQLTGINVFDDTANTGYQPIAVCA
                     SNTFSGLGHSISGLEQHNSRGTQPVVNITGGAIANTEYINTPVIRVRDGAKFPTIGLA
                     MNDAGTTAGDEIHAPAGFYRENVTVTHNGMTLSGDGASTVIDGVGLSGSGVTIPNGET
                     GATLHDFTVQNFTAIGTSSGACIYGALSNSDTAIRNTTVANCAGGRGGIFLSAGAAID
                     NVTIDNNDVSQTDGRGIVIWDGQKTHIAITNNHVHDLTGCCGIELQDGSAS"
BASE COUNT          351 a          534 c          522 g          270 t
ORIGIN      
        1 aagagcgcca atccgggtct gggcgaggtc gactacggcc tgaccgaagc ccaatcggtt
       61 gtgtacgtcg atgcgacgta cagtggccaa agcttcggga ccagcctggc attcccgttc
      121 tcgaatccga acggcgcctg cgcggcgacc actgcgtatt ttggcatcaa cgcgttcgcg
      181 acgattgccg acggcctgat gcacgtgacc aatgccggca cggtatgtat cgccaagagc
      241 aattacgccg aggcggtaaa catcaccaac aacgtgcaga tgattggtga tggcaacacg
      301 gcgggtgata ccgtcatcgc cggggcggtg acgctcaatg ccggtggcat cgtcggcgcg
      361 ccgttgttga tgcagaacct gcgcgtgacc aacgcgtcgg gcaagggcgt cgcggtgggc
      421 gcggcttcgc atttggcctt ctacggcgtt gcgttcgccg acaatggcga tgcaggtctc
      481 gacttcgatt ccgtcatcga cgatgtggcg atcagcggca gcctgttcga cgccaacacc
      541 ggcgccggca ttcgcacggc gaccaccgcg caggtgagca atgtcacgat caatggcggc
      601 actgtgttca gcaacaacgc ggccggcatc atcctgttcg gtgcgtccgg cagcggcaac
      661 ggtcagatca gcaactggac gatcgactcc agccagttcc tgagcaacga caatgccgac
      721 gccagcaatt tcggcggcgc catctggctc aagaccggtg gtgccggcag tgcgatcaac
      781 ggtttcaccg tcaccaactc catatttgcc gacaacggca gcagcaacac gctcaaccag
      841 gtcggcatca cggtgcgtgc gcgtccgggt acgaccatga cgggtgtgaa catctgcaac
      901 aacacgttcg aggagaccgc cgcgccgggc acgcagttga ccggcatcaa cgtgttcgac
      961 gataccgcca acaccggcta tcagccgatc gcggtatgcg cgagcaacac cttcagtggg
     1021 ctcggccaca gcatcagcgg cctggagcag cacaactcgc gcggcaccca gccggtggtg
     1081 aacatcacag gcggcgcgat tgcgaacact gaatacatca atactccggt gatccgcgtg
     1141 cgtgacggcg ccaagttccc gaccattggt ttggcgatga acgacgcggg cacgacggcg
     1201 ggcgacgaga tccatgcccc ggccggcttt taccgcgaga atgtcacggt cacccacaac
     1261 ggcatgaccc tgagcggcga cggcgcatcc acggtcatcg acggagtcgg cctgtccggc
     1321 agcggcgtca cgattccaaa cggcgaaacc ggcgcgacct tgcacgactt caccgtgcag
     1381 aacttcaccg cgatcgggac gtccagcggc gcgtgcatct acggggcgct gagcaatagc
     1441 gatacggcca tcaggaatac caccgtcgcc aattgcgccg gcggacgcgg cggcatcttc
     1501 ctgtcggcgg gcgcggccat cgacaacgtc acgatcgaca acaacgatgt aagccagacc
     1561 gacggccgcg gcatcgtgat ttgggacggc cagaagacgc acatcgcgat cacaaacaac
     1621 catgtgcacg acctgaccgg atgctgcggc atcgagctgc aggatggcag tgcgtcg
//
LOCUS       DASRVO010000085         1618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_132182, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000085 DASRVO010000000
VERSION     DASRVO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132182"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFF05_00990"
     CDS             complement(<1..439)
                     /locus_tag="VFF05_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50411.1"
                     /translation="MTALAVRVPHADSLNWPRVGAWSGSISIHLLIVVLLLIPGAAIQ
                     IVRHADIVPIVNVITPPPPSKPLPVLPTPAPLKVRPQTAPIAPPVISVVAASNLPVTT
                     VDIALPAAAPAPTNTATQETAPSAIAYGSRTQVAYPLEALRNRE"
     gene            complement(693..>1618)
                     /locus_tag="VFF05_00995"
     CDS             complement(693..>1618)
                     /locus_tag="VFF05_00995"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZW50412.1"
                     /translation="QVTIVMSDRGIPASYRHMHGFASHTYSFWNAKGERFWVKMHFRT
                     QQGIKNLSDAEAEAIVGKDRESHQRDLFDAIERGDFPKWKMYIQVMPETDAEKVPYHP
                     FDLTKVWPKGDYPLIEVGEFELNKNPENFFADVEQSAFAPSNLVPGIGVSPDKMLQAR
                     LFNYADAQRYRLGVNYHQIPVNAARCPVHSNHRDGAGRMDGNYGGLPHYEPNSFNQWQ
                     EQPQFREPPLKINGDADWWNYRDNDANYYKQPGDLFRLMTPAQRQVLFDNTARAMGDA
                     PEFIKRRHIDNCAKADPAYGAGVAKALGLSG"
BASE COUNT          253 a          481 c          550 g          334 t
ORIGIN      
        1 gttcgcggtt gcgcagtgcc tcgagcggat aggcgacctg cgtgcggctg ccgtaggcga
       61 ttgcactcgg ggcggtttcc tgtgtggccg tgttcgtcgg tgccggcgcc gctgccggca
      121 gagcgatatc cacggtggtg accggcagat tcgacgccgc gacgactgaa attaccggcg
      181 gtgcgatggg cgctgtttgt gggcgcacct tcaacggcgc gggggtcggc agcacaggca
      241 gcggtttgct tggcggcggc ggggtaatga cattgacgat cggcacgatg tcggcgtggc
      301 ggacgatttg aattgccgcg ccaggaatca gcagcaatac gacgatgagc aggtggatcg
      361 atatcgatcc cgaccacgcg ccgacgcgcg gccagttcaa ggaatctgca tgcggaacgc
      421 gaacagccag tgcggtcatg acacgtctcc ctgtagaggc atcacgaatt caggctagca
      481 ctgtgccggg gcccatgttt gaagaaattg ttattgcgga taccgcacgt cggcgcgatc
      541 gcgccgcgcg gtttgtgcac cgcgcggcgc aaagctcgct tcgttcaata gccgaatccg
      601 tcgacgaata tcacgtcgcc gtcgaactcc acggatccga tgtcgcattg cgtgccctgc
      661 ggcgcttgcg ctcccgggcg cgctggatca ggtcagccgg aaagtcccag cgccttcgcc
      721 acgcccgcgc cgtaggccgg gtctgccttg gcgcagttgt cgatgtggcg gcgcttgatg
      781 aactccggcg cgtcgcccat ggcgcgcgcg gtgttgtcga acaatacctg ccgctgcgcc
      841 ggcgtcatca ggcggaacag gtcgccgggc tgcttgtagt agttggcatc gttgtcgcgg
      901 tagttccacc agtccgcgtc gccgttgatc ttcagcggcg gttcgcggaa ctgcggttgc
      961 tcctgccact ggttgaagct gttcggctca tagtgcggca ggccgccgta gttgccgtcc
     1021 atgcgtccgg cgccgtcgcg gtggttgctg tgcaccggac agcgcgcggc gttgaccgga
     1081 atctggtggt agttcacgcc cagccggtag cgctgcgcat cggcgtagtt gaacaggcgt
     1141 gcctgcagca tcttgtccgg cgagacgccg atgcccggca ccaggttgct cggcgcgaac
     1201 gcgctctgct cgacgtcggc gaagaagttt tccggattct tgttgagttc gaactcgccg
     1261 acttcgatca gcggatagtc gcccttgggc cacaccttgg tcaggtcgaa cggatggtag
     1321 ggcaccttct cggcgtcggt ttcgggcatg acctggatgt acatcttcca ctttgggaag
     1381 tcgccacgtt cgatggcatc gaacaaatcg cgctggtggc tctcgcgatc cttgccgacg
     1441 atcgcttcag cctcggcgtc gctcaggttc ttgatgccct gctgggtacg gaaatgcatc
     1501 ttgacccaga agcgctcgcc cttggcgttc cagaagctgt aggtgtgcga ggcaaaaccg
     1561 tgcatgtggc ggtagctggc cgggatgccg cggtcgctca tcacgatggt gacctggt
//
LOCUS       DASRVO010000086         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_54567, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000086 DASRVO010000000
VERSION     DASRVO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54567"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFF05_01000"
     CDS             complement(<1..549)
                     /locus_tag="VFF05_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003488337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZW50413.1"
                     /translation="MNEDHPSPQPARSWLGRISQLVSGEPRNIDELLEELRDARANGL
                     LSDDTLSMVEGAIEVSDLTVADVMVPRGQIVALPADASLEEILRRVIESGHSRFPVHD
                     EDGDDIIGILLAKDLLKCFAGKDVPCNIRGLLRPVTMIPESKRLDILLKEFRLSRNHM
                     ALVVDEYGGIAGLVTIEDVLEEI"
     gene            complement(654..1115)
                     /gene="ybeY"
                     /locus_tag="VFF05_01005"
     CDS             complement(654..1115)
                     /gene="ybeY"
                     /locus_tag="VFF05_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZW50414.1"
                     /translation="MSAGLFLRNDAGRRGVPTKQSFQTWIDAIPRLPRNAQVNLLIVD
                     AHAGRRFNRQYRHKDYATNVLSFSWEPEPGAAQQSKSAALLGDLVICAPVVAREAAEQ
                     GKRPREHWAHLTIHGVLHLLGHDHETPAEARRMEALEIRILAGLGIRNPYE"
     gene            complement(1112..2086)
                     /locus_tag="VFF05_01010"
     CDS             complement(1112..2086)
                     /locus_tag="VFF05_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017174255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZW50415.1"
                     /translation="MTTLSHRDFALEPADGERLANLAGPFDEHLRQIELRLGVEIANR
                     GNVFRVNGDAAKVKLAERVLRDLYDATESETLNGAQINLRLSESGIDALEETADEAQE
                     VAIKVKRGTIRGRGANQQRYLHAVATHDINFGIGPAGTGKTYLAVAMAVDALNDSRVQ
                     RLILVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYEMIGIDRVTKLIERNVIEIA
                     PLAYMRGRTLNDAFVILDEAQNTTTEQMKMFLTRIGFGSAAVVTGDVTQIDLPRHIKS
                     GLRDAIEVLRDVDGVSFTFFTAKDVVRHPLVQKIVRAYEKRENEGTDA"
BASE COUNT          377 a          666 c          758 g          453 t
ORIGIN      
        1 gatttcctcg agcacgtcct cgatggtgac caggccggcg atgccgccgt attcgtcgac
       61 gacgagggcc atgtggttgc gcgacaggcg gaattccttg agcaggatgt ccaggcgctt
      121 ggattcggga atcatcgtca ccggccgcag caggccgcgg atgttgcacg gcacgtcctt
      181 gccggcgaag cacttgagca gatccttggc gagcaggatg ccgatgatgt cgtcgccatc
      241 ctcgtcgtgg acgggaaagc gcgaatgccc ggactcgatc acccggcgca ggatttcctc
      301 gagcgacgcg tcggccggca gggccacgat ttgtccgcgc ggaaccatga cgtcggcgac
      361 ggtgaggtcg gaaacctcga tcgcgccttc gaccatggac agggtgtcgt cggatagcaa
      421 gccgttggcg cgggcgtcgc gcaattcttc gagcagctcg tcgatgttgc ggggctcgcc
      481 ggaaaccagt tggctgatgc gtcccagcca ggaccgagcc ggctggggac tagggtggtc
      541 ctcgttcatt acattgcgtg caatgccctc acgggcggtc gcctagtgta tccgatggcg
      601 cgatttccgt aaaatatcta gctagatcaa tatgttattt gcacacgacg gaatcattcg
      661 taggggttgc ggatgcccaa tccggccagt atgcgaattt ccagggcctc catgcgccgg
      721 gcctcggccg gtgtttcgtg atcgtggcca agcagatgca gcacgccatg gatggtcagg
      781 tgcgcccagt gctcgcgcgg gcgcttgccc tgttcggcgg cctcgcgcgc gacgaccggc
      841 gcgcagatca ccaagtcgcc gagcaaagcg gccgattttg attgctgcgc cgcacccggt
      901 tcgggttccc aggagaagct cagcacgttg gtggcgtagt ccttgtgccg atactgccga
      961 ttgaagcgtc ggccagcgtg cgcatcgacg atcagcaggt tcacctgcgc gtttcggggc
     1021 aggcgcggaa tggcgtctat ccaggtctgg aaagactgct tcgtcggcac gccgcggcga
     1081 ccggcgtcgt tgcgcaggaa caacccggcg ctcatgcatc tgtgccttcg ttttcgcgtt
     1141 tctcgtaggc gcgcacgatc ttctgcacca gcggatggcg gaccacgtct ttcgcggtga
     1201 agaacgtgaa gctcacgccg tcgacatcgc gcagcacctc gatcgcgtcg cgcaggccgg
     1261 acttgatgtg ccttggcagg tcgatctggg tcacgtcgcc ggtgaccacg gcggccgagc
     1321 cgaaaccgat gcgggtgagg aacatcttca tctgctcggt cgtggtgttc tgcgcttcgt
     1381 ccaggatcac gaatgcatcg ttgagcgtgc gcccgcgcat gtaggcgagc ggcgcgatct
     1441 cgatcacatt gcgctcgatc agcttggtca cgcggtcgat gccgatcatc tcgtacagcg
     1501 cgtcgtagag cggacgcagg tagggatcga ccttttgcgt caggtcgccg ggcaggaagc
     1561 cgagtttctc gccggcttcc accgccgggc gcacaaggat caggcgctgc acgcgcgaat
     1621 cgttgagcgc atccaccgcc atcgcgaccg cgagataggt cttgccggtg ccggccgggc
     1681 cgatgccgaa attgatgtcg tgcgtggcga ccgcgtgcag gtagcgctgc tggttcgcgc
     1741 cgcggccgcg gatggtgccg cgcttgacct tgatcgcgac ttcctgtgcc tcatcggccg
     1801 tttcttccaa cgcgtcaatg cccgattcgg acaggcgcaa attgatctgc gcgccgttga
     1861 gcgtttctga ttcggtggcg tcgtagaggt cgcgcagcac gcgttcggcg agtttcactt
     1921 tcgccgcatc gccgttcacg cggaacacgt tgccacggtt ggcgatctcc acgccgaggc
     1981 gcagctcgat ctggcgcaag tgctcgtcga aagggccggc caggttggcc aagcgctcgc
     2041 cgtcggcggg ttcgagtgcg aagtcgcggt gggacagggt ggtcatattt aaggaggcag
     2101 tgggtaatac gccgatgagg aggacgcgct gcggttgttg cacgcgggtt gggaatgcgc
     2161 tagaaatcaa tgcgggcaca tcgtgccagt ttgcggcctc tgatcgattg ccgatcatgc
     2221 gcagtatttc gggaaatctt gatcgggatc aaaa
//
LOCUS       DASRVO010000087         1436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_235788, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000087 DASRVO010000000
VERSION     DASRVO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235788"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFF05_01015"
     CDS             <1..702
                     /locus_tag="VFF05_01015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZW50416.1"
                     /translation="APEIATLRALHARGTRVAATCSGTLLLAMSGLLDGHRATTSWWL
                     APTFRRHFPQVRLEEEALLVDEGDVVTTGAATAVHSLIIRMIAEVGGAELAQQVGRMM
                     LLDAERQSQAPYVSQAMLEKPRHTLSEKITAFLDKQLHNQISVTRLAAHCGTSERSLL
                     RHFRAQFGTHPLGYIQHLRVERAKALLEATQLSFDEVVERCGYSDVPSFRKLFKRETS
                     LTPADYRERFRLRAR"
     gene            complement(772..>1436)
                     /locus_tag="VFF05_01020"
     CDS             complement(772..>1436)
                     /locus_tag="VFF05_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZW50417.1"
                     /translation="IERRYRITRQILQAMLEQRHPVSIVTKNALVERDLDLLAPLAQQ
                     KLVYVFVSVTSLDNKLSSKLEPRASAPHRRIAALKALSEAGVPCGVLVAPIIPMLTDR
                     WMEQILERAREAGAQMAGYTILRLPWELKQLVREWLELHFPERAEHVLNLVRQMRGGR
                     DNDPRFGKRMRGEGEFAELIRQRFAVATRRLGVARGRDLTLDCANFIAPRKATPQGEL
                     FG"
BASE COUNT          248 a          465 c          478 g          245 t
ORIGIN      
        1 gcgccggaga tagccacgct gcgcgccttg catgcgcgcg gcacgcgcgt cgcggcgacg
       61 tgcagcggta ccttgctact ggcaatgagc ggcctgctcg atggccatcg cgccaccacg
      121 agctggtggt tggcgcccac gttccgccgc cacttcccgc aggtacggct cgaggaggaa
      181 gcgctgctcg tcgatgaagg cgacgtcgtc acgacgggtg ccgcgaccgc ggtgcactcg
      241 ctcatcattc gcatgattgc cgaagtcgga ggcgcggaac tcgcgcaaca ggtcgggcgc
      301 atgatgctgt tggatgccga acggcaaagc caggcgccct acgtcagcca ggcgatgctc
      361 gagaagccgc ggcataccct gtcggagaag atcacggcct tcctcgacaa gcaattgcac
      421 aaccagatca gcgtgacccg gctggctgcg cattgcggca ccagcgagcg cagcttgctg
      481 cgacacttcc gcgcgcaatt cggcacccat ccgttgggct acatacagca cttgcgcgtg
      541 gaacgcgcaa aggccctgct cgaagccacg cagctcagtt tcgacgaagt cgtcgaacgt
      601 tgcggctaca gcgacgtgcc gagtttccgc aagttgttca agcgcgaaac atcgttgacg
      661 ccggccgatt accgagaacg tttccggctg cgcgcgcgtt gatcgaggca acgcgcggcg
      721 aagcgcattt gcatggattg gtcgaagcgg cgtgctcgga tagggatcgc ctcacccgaa
      781 caactcgccc tgcggcgtcg ccttgcgcgg cgcgatgaag ttggcgcaat ccagcgtcag
      841 gtcgcgcccg cgcgcaacgc ctagccggcg cgtggccacg gcaaagcgct ggcggatcaa
      901 ctcggcgaat tcgccttcgc cgcgcatgcg cttgccgaag cgcgggtcgt tgtcgcgacc
      961 gccgcgcatc tgccgcacca ggttcagcac atgctcggcg cgctcgggaa aatgcagttc
     1021 gagccattcg cgcacgagct gtttcaattc ccagggcagg cgcaggatgg tgtagccggc
     1081 catttgcgcg ccggcttctc gcgcacgttc gaggatttgt tccatccagc gatcggtgag
     1141 catcggaatg atcggcgcca ccagcacgcc gcagggcacg ccggcctcgc tcagcgcctt
     1201 gagtgcggcg atgcgccggt gcggcgcgct ggcgcgcggc tccagtttgg acgagagctt
     1261 gttgtcgagc gaggtcaccg acacgaaaac gtagaccagc ttctgctgcg cgagcggcgc
     1321 gagcaaatcc aggtcgcgtt cgaccagcgc gttcttggtc acgatgctga ccgggtgccg
     1381 ctgttcgagc atggcttgca ggatctgccg cgtgatgcgg tagcggcgtt cgatcg
//
LOCUS       DASRVO010000088         1322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_132259, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000088 DASRVO010000000
VERSION     DASRVO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132259"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFF05_01025"
     CDS             <1..357
                     /locus_tag="VFF05_01025"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZW50418.1"
                     /translation="LVGGLGFAASGNIGDEVAVFEPTHGSAPKYAELDPPIVNPIAMI
                     LSAAMMLDHIGESEKAQRIRNAIAAVVKEGKVRTYDMMRIPGSGKAIAQGAASTTQLT
                     DAILKKLEAASASAAD"
     gene            441..>1322
                     /locus_tag="VFF05_01030"
     CDS             441..>1322
                     /locus_tag="VFF05_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HZW50419.1"
                     /translation="MATANKTSTAASAGRIGPDVRSDCKVSFEPRASGGLEIELQSKV
                     AAYYGASIREQAAEVLGHLGVEHSRLLIEDAGALPFVLAARIEAAVRRAGCAFGRQAL
                     PARVPLPPPSPRDRLRRSRLYLPGSEPKYFVNAALYHPDAIILDLEDSVHHAEKDAAR
                     ILVRNALAAVDFVAAGVPADGGPWARCERMVRINQLPLGLEDLDEIVPEQPDVILIPK
                     VENEQQVKAVDLRIKEIQERLRRERPIWLMPILESAIGIEHALEIANASPRVCALTIG
                     LEDYTADLGVAKTTGGAE"
BASE COUNT          240 a          420 c          459 g          203 t
ORIGIN      
        1 ctggtgggcg ggctgggctt cgcggccagc ggcaacatcg gcgacgaagt cgcggtattc
       61 gagccgacgc acggttcggc gccgaagtac gcggaactgg atccgccgat cgtcaatccc
      121 atcgccatga tcctgtcggc ggcgatgatg ctggaccaca tcggcgaaag cgagaaggcg
      181 cagcgcatcc gcaacgcgat tgccgcggtg gtgaaggaag gcaaggtgcg cacctacgat
      241 atgatgcgca tcccgggcag cggcaaggcg atcgcgcagg gcgcggcttc caccacgcaa
      301 ttgacggatg cgattctgaa gaagctggag gcggcgagcg ccagcgcggc agactgaaag
      361 caaccgctga tcacgctgat caacgctgat tatttttact tcatccgcga ccatcagcga
      421 aatcagcggt gaggattgaa atggcgaccg caaacaaaac gagcaccgcc gcaagcgccg
      481 ggcgaatcgg accagatgtc cgctcggact gcaaggtcag cttcgagccg cgcgcgtccg
      541 gcggccttga gattgagttg cagtcgaagg tcgcggcgta ctacggcgcc agcatccgcg
      601 agcaggcggc ggaagtcctg gggcatctag gggtagagca ctctcgactg ctgatcgagg
      661 acgcgggcgc gctgccgttc gtgctggcgg cgcggatcga ggccgcggtg cggcgcgccg
      721 ggtgcgcctt cgggcgccag gcgcttcccg cgcgcgtgcc actgcccccg ccgtctcctc
      781 gcgaccgcct gcgccgttcg cgcctctatc ttcccggcag cgagcccaag tattttgtta
      841 acgccgcgct gtatcacccc gacgccatca ttctcgatct cgaggattcc gtgcaccacg
      901 cggaaaaaga cgcggcgcgc atcctggtgc gcaacgcgct ggcggcggtg gacttcgtgg
      961 ccgcgggagt gccggccgac ggcgggccgt gggcgcgctg cgagcgcatg gtgcgcatca
     1021 accagttgcc tcttggcctg gaggatctgg acgaaatcgt gcccgagcag cccgacgtca
     1081 tcctcattcc caaggtggag aacgagcagc aggtgaaggc ggtggacttg cgcatcaagg
     1141 aaatccagga gcggctgcgg cgcgagcggc ccatctggct gatgccgatc ctggagtccg
     1201 ccatcggcat cgagcacgct ctggaaattg ccaacgcgtc gccgcgggtg tgcgcgctga
     1261 ccatcggcct cgaggactac accgccgacc tgggcgtggc caagaccacg ggcggcgcgg
     1321 ag
//
LOCUS       DASRVO010000089         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_106183, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000089 DASRVO010000000
VERSION     DASRVO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106183"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..524)
                     /locus_tag="VFF05_01035"
     CDS             complement(162..524)
                     /locus_tag="VFF05_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZW50420.1"
                     /translation="MIRFALILVLCAPALAAAAPADDCGVSGTAYDFAGRPLRAAVVR
                     LTDTQTQRAAFIVTDASAAFVFADLAPGARYRVDLLSEPTVVTGSRLPTRSIVGMSSA
                     FTCMAGSTARTDVRAQVY"
     gene            complement(618..>2154)
                     /locus_tag="VFF05_01040"
     CDS             complement(618..>2154)
                     /locus_tag="VFF05_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZW50421.1"
                     /translation="NAMAGTLSSVIQSQALAALFLPIASRVSARTGVPLSRLLLPMAF
                     CILCGTNMTLISNSPLILLNDLIASANRNLPPGAQTIEPFSLFAITPIGIPLLLTGLA
                     YFALFTRKLLPGDDDDDQSVTPGRTETYFAETYGIDGEVSELTVTTESPLVGMSIAEA
                     EALRGAPLILAIKNGDETRLAPPADTMIWVGTVLGTLGKREQVNEFANLNLLRAQSRL
                     SVFRDMFNPNRAGIAEAVVPPNSRFVKQAVGDLRLRKRFGISVLAVYRGDAVFREDVR
                     KTALRAGDTLVLHGFWRDLTLAAEERDFVVVTDYPKEDHRPSKIWHAVTFFALAMSLG
                     LFTDMKLSVAMMTGAVGMLLAGVLNIDEAYAAVNWKTIFVMACLIPLGAAMDSTGAAA
                     WLAQELIAHTGHMPQWILQTCVAILALLFSQVISHVGTAVMMVPMAINLALASGGNPS
                     VYALIVAMSVSNTFLIPGASPTILVVAGPGGYRQKDFLRVGLPLTVLMLVVMLVAVNL
                     LFR"
BASE COUNT          398 a          656 c          754 g          346 t
ORIGIN      
        1 gcaaattcct gatcgacgcg ggacaactga tttgcgccgc gcacggcgca accttccgga
       61 tcgaatccgg acattgcagc ggcggtcctt gccgcggcga tgcgttgcgc tcggtgaagg
      121 tcgaggtgat cgacgggcag gtctgcctgc cctgaatcgc ttcagtagac ctgcgcgcgc
      181 acgtcggtgc gggccgtgct tcctgccatg caggtgaacg cggacgacat gccgacgatc
      241 gaccgtgtcg gtaggcgcga accagtgacg acggtcggtt cactgagcag atcgacgcgg
      301 tagcgcgcac cgggcgcgag atcggcgaag acgaaagcgg cgcttgcatc ggtgacgatg
      361 aacgcggcgc gctgggtctg cgtgtcggtc aggcgcacga cggcggcgcg aagcggccgc
      421 ccggcgaaat cgtaggcggt gccggaaacg ccgcagtcat cggcgggcgc ggcggcggcg
      481 agtgcgggag cgcaaagaac cagaatcaag gcgaaacgga tcatggcgaa cctccggtga
      541 tgcgatgcgc atccgtgcgg agcggattcc tgtggcgatt ctagcgcggc cggcgaagac
      601 cggcgtaacc gggccggcta gcggaacagc agattgaccg ccaccagcat caccaccagc
      661 atcagtacgg tcaacggcag gcccacgcgc aggaaatcct tctgccggta gccgcccggg
      721 ccggcgacca cgaggatcgt gggactggca ccgggaatga ggaaggtgtt ggacaccgac
      781 atcgccacga tcagcgcgta cacgctcggg ttgccgccgg aagccagggc aaggttgatc
      841 gccatcggca ccatcatcac ggccgtgccc acatgcgaga tcacctgcga gaacaaaagg
      901 gcgaggatcg cgacgcaggt ctgcaggatc cattgcggca tgtgaccagt gtgcgcgatc
      961 aattcctgag ccaaccatgc cgcggcgccg gtcgaatcca ttgcggcgcc gagcgggatc
     1021 aggcaggcca tcacgaagat cgttttccag ttcaccgccg cgtaggcttc gtcgatgttg
     1081 agcacgccgg ccagcagcat gccgaccgca ccagtcatca tcgccaccga cagcttcatg
     1141 tcggtgaaca gacccaggct catggccagc gcgaagaaag tcaccgcgtg ccagatcttg
     1201 ctcgggcgat ggtcttcctt cgggtaatcg gtgaccacga cgaagtcgcg ttcttccgcg
     1261 gccagggtga ggtcgcgcca gaaaccgtgc aggaccaggg tgtcgcccgc gcgcagggcg
     1321 gtcttgcgca cgtcttcgcg gaacaccgcg tcgcctcggt aaaccgcgag cacgctgatg
     1381 ccgaagcgtt tgcgcaggcg caggtcgccg accgcctgtt tgacgaagcg cgaattcggc
     1441 ggcaccacgg cttcggcgat gccggcgcgg ttggggttga acatgtcgcg gaacacgctc
     1501 agacgcgact gcgcgcgcag caggttgagg ttggcgaatt cgttgacttg ttcgcgcttg
     1561 ccgagcgtgc cgagcacggt gccgacccag atcatggtgt cggccggagg cgccaggcgg
     1621 gtttcgtcgc cgttcttgat cgcgaggatc agcggtgcgc cgcgcagggc ctcggcctcg
     1681 gcgatgctca tgccgaccaa cgggctttcc gtggtgacgg tgagctcgct gacttcgccg
     1741 tcgatgccgt aggtttcggc gaagtaggtt tcggtgcggc ccggcgtcac gctctggtcg
     1801 tcgtcgtcgt caccgggcag cagcttgcgc gtgaacaacg caaagtacgc cagtcccgtc
     1861 aacagcaggg ggatgccgat cggagtgatc gcgaacaacg agaacggttc gatggtctgc
     1921 gcgcccggcg gcaggttgcg gttggcgctg gcgatcaagt cgttgagcag gatcagcggt
     1981 gaattgctga tcagcgtcat gttggtgccg cacagtatgc aaaacgccat cggcagcagc
     2041 aggcggctga gcggaacgcc ggtgcgtgcg ctcacgcgac tggcgatagg caggaacaat
     2101 gcggcgagcg cctggctctg gatcaccgac gacaaggttc cggccatcgc gttg
//
LOCUS       DASRVO010000090         1085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_262380, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000090 DASRVO010000000
VERSION     DASRVO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262380"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFF05_01045"
     CDS             <1..355
                     /locus_tag="VFF05_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HZW50422.1"
                     /translation="FEGTPAQMLSSLDKLSALPGATRVCCTHEYTLSNLNFARAVEPG
                     NAELMQYVQHCQALRARDVPTLPSTIAREQQINPFLRARVPAVAQAARAHDAATPPDA
                     VGVFATLRQWKNEFK"
     gene            352..>1085
                     /locus_tag="VFF05_01050"
     CDS             352..>1085
                     /locus_tag="VFF05_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZW50423.1"
                     /translation="MKLLLAAALAASLWLAGCATPPATTADHAAASKGTPGAGSASAV
                     PAAPAVIPGGPLKPIASADLSSQAVAAMRPPIDLWDRIRRGFAMPNLDNALVHQQEQW
                     YASRPDYIQRMTERSSKYLFHIVEELERRNMPTELALLPFVESAFNPQAVSSARAAGM
                     WQFMPATGKTFELKQNVFRDDRRDVLASTRAALDYLQKLYGMFGDWHLALAAYNWGEG
                     SVSRAVARNQTNGLGTSYSELNMPNET"
BASE COUNT          182 a          391 c          345 g          167 t
ORIGIN      
        1 gttcgagggc acgccggcac agatgctgtc ctcactggac aagctgtcgg cgctgcccgg
       61 cgccacgcgc gtgtgctgta cgcacgaata cacgctttca aacctgaatt tcgcgcgcgc
      121 agttgaaccg ggaaacgccg aactgatgca gtatgtgcaa cattgccagg cgctgcgcgc
      181 gcgcgatgtg cccactctgc cttcgaccat cgcgcgggag cagcagatca atccgttcct
      241 gcgcgcgcgc gttcccgccg tcgcgcaggc tgcccgcgcg catgatgccg cgaccccgcc
      301 ggatgcggtc ggcgtattcg ccaccctgcg gcaatggaag aacgagttca aatgaagctg
      361 ctcctcgccg cggccctggc tgcgtcgctc tggctcgccg gctgcgccac gccgccggcc
      421 accaccgccg atcacgccgc cgcgagcaag ggcacgccgg gggccggttc cgccagcgcc
      481 gtcccggccg cgcctgcggt cattcccggt ggcccgctca agccgatcgc ctcggccgac
      541 ctgtcgtcgc aggcggtcgc agcgatgcgg cctccgatcg acctgtggga tcgcattcgc
      601 cgcggcttcg cgatgcccaa cctggacaac gcgctggtgc accagcagga gcagtggtac
      661 gcgagccggc ccgactacat ccagcggatg accgagcgtt ccagcaagta cctgttccac
      721 atcgtcgagg agctcgagcg gcgcaacatg ccgaccgaac tggcgctgct gcctttcgtc
      781 gaaagcgcgt tcaatccgca ggcggtgtcg agtgcacgcg cggccggcat gtggcagttc
      841 atgcctgcga ccggcaagac cttcgagctg aagcagaacg ttttccggga tgaccgccgc
      901 gacgtcctgg cctcgacgcg cgccgccctc gattacctgc agaagctcta cggcatgttc
      961 ggcgactggc acctggcgct ggctgcctac aactggggcg aaggcagcgt cagccgcgcc
     1021 gtggcgcgca accagacaaa cggcctgggc acctcctaca gcgaactgaa catgcccaac
     1081 gagac
//
LOCUS       DASRVO010000091         1237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_210116, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000091 DASRVO010000000
VERSION     DASRVO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210116"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1048
                     /gene="pdxA"
                     /locus_tag="VFF05_01055"
     CDS             62..1048
                     /gene="pdxA"
                     /locus_tag="VFF05_01055"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZW50424.1"
                     /translation="MNDRPFLPRLAVTIGEPAGIGPELVATLAATDLGADLIAIGDKD
                     SLLLAAQTRGIALKLEADDGQWRRERAAGSLRYVHIPAPAPVVPGKLDVRNAPYVVAM
                     LARAADGCLEGEFDALVTAPVQKSIVNDAGITFTGHTEFFAERANCDVAMLLVTKGLR
                     VALATTHLPLAEVPGAITAAGLVRILRIVDNDLRHRFRIENPHIAVLGLNPHAGEGGH
                     LGREEIDIIAPTLAALRADGMRLEGPLSADTAFVPDQRTRFDAYLAMYHDQGLPVLKA
                     LGFGDSVNVTLGLPFVRTSVDHGTALDLAGSGKADPASLIAAVKLAMELARA"
     gene            1045..>1237
                     /locus_tag="VFF05_01060"
     CDS             1045..>1237
                     /locus_tag="VFF05_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HZW50425.1"
                     /translation="MNAPAHRPRKRFGQHFLHDPGILRRIVDAIAPRPGQHLVEIGPG
                     EGALTLPLLRAAGKLTAIEL"
BASE COUNT          209 a          446 c          388 g          194 t
ORIGIN      
        1 cgcggagaac cagccggaaa cacaaaagcc ggctccctga ttttcgtttg aacgcgcagc
       61 cgtgaacgac cggccatttt tgccgcgact ggccgtcact atcggcgaac ccgccggcat
      121 cggtccggaa ctcgtcgcca cgcttgcggc gaccgatcta ggcgccgacc tgatcgccat
      181 cggcgacaag gattcgctcc tgctcgccgc gcagacgcgc ggcatcgcgc tgaaactcga
      241 agccgacgac ggccaatggc gtcgcgaacg cgcggcgggt tcgctgcgct acgtgcacat
      301 tcccgcgccg gcgccggtcg ttcccggcaa actcgacgtg cgcaatgcgc cgtacgtcgt
      361 cgccatgctg gcgcgcgccg cggacggctg cctcgaaggc gaattcgatg cgctggtcac
      421 cgcgcccgtg cagaaaagca tcgtcaacga cgccggtatc acgtttaccg ggcacacgga
      481 gtttttcgcc gagcgcgcca attgcgacgt ggccatgctg ctggtaacga aaggcctgcg
      541 tgtggcgctg gcgaccacgc acctgccgct ggcggaagtt cccggcgcga ttaccgccgc
      601 tggactcgtg cgcatcctgc gtatcgtgga caacgacctg cgccaccgtt tccgcatcga
      661 gaacccgcac atcgcggtgc tcggactgaa cccacacgcc ggcgaaggcg gacacctcgg
      721 ccgcgaggaa atcgacatca ttgcacccac gctcgccgcg ctgcgcgccg acggcatgcg
      781 cctggaagga ccgctgtccg ccgacaccgc gttcgtgccg gatcagcgca cgcgcttcga
      841 cgcgtatctc gcgatgtacc acgatcaggg cctgcccgtg ctcaaggcct tgggtttcgg
      901 cgactcggtc aacgtgacgc tcggcctgcc gttcgtgcgc acctcggtcg atcacggcac
      961 tgcgctcgat ctcgccggca gcggcaaagc ggaccccgcc agcctgatcg ccgcggtgaa
     1021 gctggcgatg gaactcgcgc gcgcatgaac gcgcccgcgc atcgcccgag aaagcgtttc
     1081 ggccagcatt tcctgcacga tccgggcatc ctgcgccgca tcgtcgatgc gattgcgccg
     1141 cggccaggac aacacctcgt cgagatcggt cccggcgaag gcgcgctgac gctgcccttg
     1201 ttgcgcgccg cgggaaaact caccgccatc gaactcg
//
LOCUS       DASRVO010000092         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_2470, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000092 DASRVO010000000
VERSION     DASRVO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VFF05_01065"
     CDS             <1..326
                     /locus_tag="VFF05_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZW50426.1"
                     /translation="ADRILAAIDSRGGRVAIKGWPELTVLTPAAAIRALEPYCAGFLY
                     THIDSEGLMQGIPLDVVRELRALTGREFIAAGGIRSHDEVAALHALGVDAVVGMALYT
                     GAMQA"
     gene            374..>1007
                     /locus_tag="VFF05_01070"
     CDS             374..>1007
                     /locus_tag="VFF05_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZW50427.1"
                     /translation="MKNRQQDPLTAERFPSAVYAETVLAVNFEDAKKYFLSALLDIHY
                     AHTLMLERQGIISAADAATILRALDGVDRSRIHASSYTGEFEDLFYFVEETLTAAIGA
                     DIAGKMHTARSRNDIDLTLYRMEVRKELLALAGEALDATRALLDLAEANIETIIPAYT
                     HTQPAQPSTIAHYLLAVVEQLERDTVRLRAAYTSTNRNPLGACAITGTAFP"
BASE COUNT          185 a          353 c          295 g          174 t
ORIGIN      
        1 gcgccgaccg tatcctggcc gccattgatt cccgcggcgg ccgcgtcgcc atcaaaggtt
       61 ggcccgaact gaccgtgctc acgcccgccg ccgccatccg cgccctggaa ccctactgcg
      121 ccggatttct gtacacgcac attgacagcg aaggcctgat gcagggcatt ccgctcgacg
      181 tggttcgcga gcttcgcgcc cttaccgggc gcgaattcat tgccgccggc ggcatccgca
      241 gccacgacga agtggcggct ctccacgcgc tcggcgtgga cgcggtggtc ggcatggcac
      301 tctacacggg cgcgatgcaa gcctgattct ttatactgag atttccccgg cggttgcggg
      361 aagggagccc caggtgaaaa acaggcagca agacccactg acggcggagc gcttcccctc
      421 ggcggtgtat gccgagacgg tgctggccgt caatttcgaa gacgccaaga agtattttct
      481 ttccgcgctg ctcgacatcc actacgcgca cacgctcatg ctggagcggc agggaatcat
      541 ttctgcggcc gacgccgcca ccatcctccg cgcgctcgac ggcgtggacc gcagccgcat
      601 tcatgcatct tcttataccg gcgaattcga ggacctgttt tacttcgtgg aagaaacgct
      661 gactgccgcc atcggcgccg acattgccgg caagatgcac accgcgcgca gccgcaacga
      721 cattgacctc acgctctacc gcatggaagt gcgcaaggaa ctgctggcgc tggccgggga
      781 agctctcgac gccacgcgcg cgctgctcga cctcgccgaa gccaatattg aaaccattat
      841 tccggcctac acccataccc agccggcgca gccgtccacg atcgcgcatt acctgctcgc
      901 ggtcgtcgag cagctcgagc gcgacacggt ccgcctgcgc gccgcgtaca cgtcgacaaa
      961 tcgcaacccg ctcggcgcgt gtgcgatcac gggaacggcg tttccaa
//
LOCUS       DASRVO010000093         1251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_54705, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000093 DASRVO010000000
VERSION     DASRVO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54705"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFF05_01075"
     CDS             <1..302
                     /locus_tag="VFF05_01075"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW50428.1"
                     /translation="KDQAEALTLIEKALTLKPDEPAIIDSMGWVQYRLGHLEQAVERL
                     RTAYAKQPDPEIAAHLGEVLWVSGQKDEAKKIWAQGQKKDAKNKVLLETMKRLES"
     gene            326..916
                     /gene="lolB"
                     /locus_tag="VFF05_01080"
     CDS             326..916
                     /gene="lolB"
                     /locus_tag="VFF05_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HZW50429.1"
                     /translation="MRLVLALTLLALAACAPVRVRETPAALAAQQAREARLAPMTHWT
                     LNAHIGVSNRGDGGSGDLTWEQDGEAFHFTVRAPVTGKTWTLSGDAGHAVLEGVDPQP
                     DTDSDAQRLLHDRLGWDVPLADLRTWVRGLRAPGSAASVQYDERNLPAVIEQDGWRVE
                     YRDWFIDRDPPLPRKVFANRADARVRMAIESWSVDE"
     gene            909..>1251
                     /locus_tag="VFF05_01085"
     CDS             909..>1251
                     /locus_tag="VFF05_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016900689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZW50430.1"
                     /translation="MSDGWTAWPAPAKLNLFLHIVGRRADGYHRLQTVFQLLDRGDTI
                     RLRVREDGVIARVAGLENVAAEDDLVVRAARALQAATGRKLGADIAVDKRIPTGAGLG
                     GGSSDAATVLVA"
BASE COUNT          230 a          393 c          429 g          199 t
ORIGIN      
        1 ccaaggacca ggccgaggcg ctgaccctga tcgaaaaggc gctgactttg aaaccagacg
       61 aaccggccat catcgacagc atgggctggg tgcagtaccg gctcggccat ctcgaacagg
      121 ccgtcgaacg cctgcgcacg gcctacgcga agcagcctga tccggaaatc gccgcgcacc
      181 tgggcgaggt gctgtgggtt tctggccaaa aggacgaagc gaaaaaaatc tgggcgcagg
      241 gacagaagaa ggacgcgaag aacaaggtgt tgctggaaac catgaagcgc ctcgaatcgt
      301 gacgtatacg tttccacaat tttccgtgcg cctcgtcctg gcactgacgt tgctcgccct
      361 cgccgcctgc gcgcccgtgc gcgtgcgcga gacgcccgcc gcgctggccg cacagcaggc
      421 gcgcgaggcg cggcttgcgc cgatgacgca ttggacgttg aacgcgcata tcggcgtatc
      481 caatcgcggc gacggcggca gcggcgacct aacctgggaa caagacggcg aagcattcca
      541 tttcaccgtg cgtgcgccgg tcaccggcaa gacctggacg ctgagtggcg atgccggcca
      601 cgccgtactc gagggcgtcg atcctcagcc ggatacggac agcgatgcac aacgcctgct
      661 gcatgatcgc ctcggctggg acgtgccgct ggcggatttg cgcacctggg tgcgtggtct
      721 gcgtgcgccg ggttcggccg cgtccgtgca atacgacgag cgtaatctgc cagcggtgat
      781 cgaacaggat ggttggcggg tggaataccg cgactggttc atcgaccgcg atccgccgct
      841 gccgcgcaag gtgttcgcga accgcgccga tgcgcgcgtg cgcatggcga tcgaaagctg
      901 gagcgtcgat gagtgacggt tggaccgcct ggcccgcacc ggccaagctc aatctgttcc
      961 tgcacatcgt cggcaggcgc gcggacggct atcaccgctt gcagaccgta ttccaactgc
     1021 tcgatcgggg cgacacgatc cgcttgcgcg tgcgcgagga cggcgtgatc gcgcgcgttg
     1081 ccggactgga aaatgtggct gcggaagacg atctcgtcgt gcgcgcggcg cgcgccctgc
     1141 aagccgcgac cggccgcaaa ctcggcgcgg acattgccgt agataagcgc attccgaccg
     1201 gcgcaggtct gggcggtggc agttcggatg ccgcgacggt actggtcgcg t
//
LOCUS       DASRVO010000094         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_314296, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000094 DASRVO010000000
VERSION     DASRVO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314296"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VFF05_01090"
     CDS             <1..934
                     /locus_tag="VFF05_01090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZW50431.1"
                     /translation="LAHAREAAKSAVDYADKQDKAKSVADAYDVLYQVAKRQGDDAAA
                     LAWHEKYAAADKAYLNDVSARALAYQMVHQRVLDNQRQLEAATDRNKLLSLQQQIDAQ
                     KARSRLLYILLLLSGLLIVGGWAYRTKRSQIKFQKLARRDGLTGIFNRQHFFESAQEV
                     LRYCAKASREACVVVMDLDHFKSVNDMHGHAAGDDALKRAVAASQVSLRSIDVFGRLG
                     GEEFAILFPDCPLAVARQRADAMRESIAASRREGDAAADVLVTASFGVAATQDCGYNL
                     ATLLAAADDAMYTAKRAGRNRVNVYRGAESVAAT"
     gene            1067..1348
                     /locus_tag="VFF05_01095"
     CDS             1067..1348
                     /locus_tag="VFF05_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50432.1"
                     /translation="MSNIIGFLEQAGMNAAMRHAGREALLRAMREEEIVPVQQRAMLH
                     AQRSVLDDLTGAREKMYCKNTAIKQPKKKPAKKAPAKKPAKKAPAKRRK"
     gene            1420..>1968
                     /locus_tag="VFF05_01100"
     CDS             1420..>1968
                     /locus_tag="VFF05_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50433.1"
                     /translation="MLVAFALAAGLRVFAASESIDERLQRADVLKTADNSEFQAVIKQ
                     LDAESGALTTAQHDFLDYLHAWQLGYLGQYDDAVSAFNRLLARTQDPTVRARSLISLI
                     NDQVNDADYENAYTNLNRLLDSLPRVTDHTANFLSLIIAGILYQAAGQTDLALRYDDL
                     ALNYDHDDRATCFALSNKIEALA"
BASE COUNT          387 a          663 c          597 g          321 t
ORIGIN      
        1 cctggcccac gcgcgcgagg ccgcaaaaag cgcggtcgat tacgccgaca agcaggacaa
       61 ggccaagtct gttgccgatg cctacgacgt gctctaccag gttgccaagc gccaaggcga
      121 cgatgcggcc gcgctggcct ggcacgagaa atacgccgcc gccgacaagg cctatctcaa
      181 cgacgtcagc gcacgcgcgc tggcctacca gatggtgcac cagcgcgtgc tcgacaacca
      241 gcgccagctc gaagcggcta ccgaccgcaa caagctgctc tccctgcagc agcagatcga
      301 cgcgcagaag gcgcgctcgc gcctgctcta catcctgctc ctgctcagcg gcctgctcat
      361 cgtcggcggc tgggcgtacc gcaccaagcg ctcgcagatc aagttccaga aactggcacg
      421 gcgcgatggc ctgaccggca tcttcaaccg ccagcacttc ttcgagtccg cccaggaagt
      481 gctgcgctat tgcgccaagg cctcgcgcga ggcctgcgtc gtggtgatgg accttgatca
      541 cttcaagtcg gtcaacgaca tgcacggcca cgccgctggc gacgacgcac tcaagcgtgc
      601 ggtcgcagcc agccaggtgt cgctgcgttc gatcgacgtg ttcggccgcc tcggcggcga
      661 agagttcgcc atcctgtttc ccgactgccc gctcgcggtc gcgcgccagc gcgccgacgc
      721 catgcgcgag tccatcgccg cctcacgccg cgagggcgat gccgccgcgg acgtgctggt
      781 gaccgccagc ttcggcgtgg ccgccacgca ggactgcggc tacaacctcg ccaccctgct
      841 cgccgccgcc gacgatgcca tgtacaccgc caaacgcgct ggtcgcaatc gggtcaacgt
      901 gtatcggggt gccgagtccg tcgccgcgac ctgaaaccgg gcgccttccg aagttgtgcc
      961 gactgcgccg ccaacgcgag actggcgtgg atcgttgcgg atatgtcacg attccgcggc
     1021 ggccgtgcag gcgcaatgtt tcaaaccgac catcaagggg aagaaaatgt cgaacatcat
     1081 cggatttctg gaacaagccg gaatgaacgc ggccatgcga catgccggcc gcgaggcgct
     1141 gctgcgcgcg atgcgcgagg aagaaatcgt acctgtacag cagcgtgcga tgctgcacgc
     1201 acagcgttcg gtcctggacg atctgaccgg cgcgcgcgag aagatgtatt gcaagaacac
     1261 cgcgatcaaa caaccgaaaa agaaaccggc aaagaaggct ccggccaaga agcccgccaa
     1321 gaaggcgccg gccaagcgca ggaaatgacc cacgggccct gaaaggccgt gctggcgcgt
     1381 ttcgtttcag gatggcgccc ggcccgttgg gccgggcgtg tgcttgtcgc cttcgcgttg
     1441 gcggccggtt tgcgcgtttt tgccgcgtcg gaaagcatcg acgagcggct gcaacgcgcc
     1501 gacgtcctta aaacggccga caactccgaa ttccaggctg tcatcaagca gctcgacgcg
     1561 gaatccggcg cgctgacgac cgcacagcac gactttctcg attacctgca tgcctggcaa
     1621 ttgggttatc tggggcaata cgatgacgcc gtgtcggcgt tcaatcgcct tctcgcgcgc
     1681 acacaggacc cgaccgtgcg tgccaggtcc ctgatctcgc tgatcaacga tcaggtcaat
     1741 gacgccgatt atgaaaacgc ctataccaac ctgaaccgat tgctggattc cctgccgcgc
     1801 gtgactgatc acacggcgaa ttttctaagc ttgatcatcg ccggaatcct gtaccaagcg
     1861 gccgggcaga ccgacctcgc gctgcgttat gacgatcttg cgctgaacta cgaccacgac
     1921 gaccgcgcca cctgttttgc gctgtcgaac aagatcgagg ccctcgcg
//
LOCUS       DASRVO010000095         1227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_2530, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000095 DASRVO010000000
VERSION     DASRVO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2530"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFF05_01105"
     CDS             complement(<1..597)
                     /locus_tag="VFF05_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50434.1"
                     /translation="MDGLLGEFGSGDAQTRAESWRYSRLALRTLEQLSFQNAADNAQL
                     PSALIERFDWPQTRGHRLVFVNGVLDASHSDAGTSGAIIETAADGACTIRFRGKGVLH
                     LVFANIATDAPSRWNATLHIHVESGKAYLAEQHVGTEGADVLGALISQITIDEGAALQ
                     TATLGDLPGSVSLVRRTRIALGNDAGFDATHALCGGRFQ"
     gene            complement(599..>1227)
                     /gene="sufC"
                     /locus_tag="VFF05_01110"
     CDS             complement(599..>1227)
                     /gene="sufC"
                     /locus_tag="VFF05_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009591996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HZW50435.1"
                     /translation="DGYEVTAGSVTYNGIDLLQLSPEARAAAGVFLAFQYPVEIPGVN
                     NTYFLKAALNAQRKARGEAELDAMQFLKLVRAKLAVLQLKEDLLNRAVNAGFSGGEKK
                     RNEIFQMALLEPKLAILDETDSGLDIDALKMVADGVNAMRAPDRAFVVITHYQRLLDY
                     IVPDFVHVLADGRIVESGDAALALKLEEHGYAWIAQRLAPVEHRVGAA"
BASE COUNT          219 a          388 c          399 g          221 t
ORIGIN      
        1 ctggaaacgc ccaccacaca gcgcatgggt ggcatcgaat ccggcatcgt tgccgagcgc
       61 aatccgggtg cgccggacca gcgaaaccga accgggcaga tcgccgagcg tcgccgtttg
      121 caaggctgcg ccctcgtcaa tcgtgatttg agaaatcagt gcgccgagta cgtccgcgcc
      181 ttccgtgccg acatgttgtt ccgcgaggta ggccttgccg gattcgacgt ggatgtgcag
      241 cgtcgcgttc cagcgcgatg gcgcatccgt cgcgatattg gcgaatacca gatgcagcac
      301 gcccttgcca cgaaagcgga tcgtgcacgc gccgtcggcc gcggtctcga tgatcgcgcc
      361 ggatgtgccc gcatccgaat gtgaggcatc gaggacgcca ttgacgaaaa caaggcgatg
      421 gccgcgcgtc tgcggccagt cgaagcgctc gatcagcgcc gacggcaatt gcgcattgtc
      481 agcggcgttt tggaatgaca gttgttccag cgtgcgcagc gcgaggcggc tgtagcgcca
      541 cgactccgcg cgcgtttgtg catcgccgct gccgaattcg ccgagcaggc cgtccatgtc
      601 aggcagctcc gacccgatgc tcgacgggag ccaagcgttg cgcaatccag gcgtagccgt
      661 gttcttcgag cttgagtgcg agcgccgcat cgccggattc gacgatgcgg ccatcggcca
      721 gcacatgcac gaaatccggc acgatgtagt cgagcaggcg ctggtaatgc gtgatcacga
      781 cgaaggcgcg atccggcgcg cgcatcgcgt tgacgccgtc ggcgaccatc ttcagcgcgt
      841 cgatgtccag gcccgaatcg gtttcgtcga ggatcgcgag tttcggctcg agcaacgcca
      901 tctggaaaat ctcgttgcgc ttcttctcgc cgccggagaa accggcattg accgcgcgat
      961 tgagcaggtc ctctttcagt tgcagcacgg cgagcttcgc gcgcaccagt ttgaggaact
     1021 gcatcgcgtc gagctcggcc tcgccgcgcg ccttgcgctg tgcgttgagc gcagccttga
     1081 gaaaataggt gttgttgacg ccggggattt ccaccgggta ctggaacgcc aggaacacgc
     1141 cggccgccgc gcgcgcttcc ggcgaaagct gcagcaggtc gatgccgttg taggtcaccg
     1201 agccggcggt cacctcgtaa ccatcgc
//
LOCUS       DASRVO010000096         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_288502, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000096 DASRVO010000000
VERSION     DASRVO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288502"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VFF05_01115"
     CDS             <1..398
                     /locus_tag="VFF05_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZW50436.1"
                     /translation="IADIPGLIEGAADGAGLGIQFLKHVSRTRLLLHLVDMAPLDDAV
                     DPVEQVHAIEAELEKFDPELLERPRWLVFNKADLLDPKERDKLAKAIVRKLKWKAPWF
                     VVSAIAKEGTWDVCLKIQAFFDALRSEQA"
     gene            complement(458..718)
                     /gene="rpsT"
                     /locus_tag="VFF05_01120"
     CDS             complement(458..718)
                     /gene="rpsT"
                     /locus_tag="VFF05_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003281567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZW50437.1"
                     /translation="MANIKSAKKRARQAVKRNARNASSRSMLRTAIKKVVNAIAAKDK
                     PGAEAAYKAAEPVMDRYSSRGLIHKNKASRHKSRLTAHIKAL"
     gene            949..2556
                     /gene="murJ"
                     /locus_tag="VFF05_01125"
     CDS             949..2556
                     /gene="murJ"
                     /locus_tag="VFF05_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZW50438.1"
                     /translation="MKAPSLLRGVFSFSAMTMISRVLGLARDVAISHVFGANAATDAF
                     VIAFRIPNFMRRLFAEGSFSTAFVPVFTQVKETRSHAELRELTARVAGTLGGALLIIT
                     ALGLIFAPQLAMTFAPGALEEPGKLGLTTDLLRLTFPYLLFVSLTALAGGVLNSFHRF
                     ALPALAPVILNICMIAGALWLAPHLQVPILSLGWAVLVAGVLQLLFLLPALRGMNLLT
                     LPRWGWKHPQVRRIMRLMLPTLFGSSIAQLNLLLDTIVISLLATGSQTWMYQATRFLE
                     LPLGVFGVALGTVILPALSRHHVAEDGAGFSRALDWGLRTTLLIAVPAMCALMILAEP
                     LVATFFQNGQYTAFDTHMTAIAIVALGTGVPAIALTRTLLPAFYSRQDTQTPVRAGVA
                     ALVVNMVFNFILIAALFEAWAAPDLKSVPWLDGIARLPGLHLGLAIASSVSNYLQFAL
                     LWRYLKRAGVYTRQSGWTRHWLRLGIASAAMMAVLAVGVWLWPDWTTVRIVTRGWHLA
                     VLVVAAACAYVAVLFGTGFRMRDLHAV"
     gene            2595..3554
                     /locus_tag="VFF05_01130"
     CDS             2595..3554
                     /locus_tag="VFF05_01130"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZW50439.1"
                     /translation="MNWLYRDAAGQCLAPRGSAVCIGAFDGVHRGHQSVLAQVRASAQ
                     AQGLTPVAVSFDPIPRVFFAREKGVPQLSSTREKIALLRQAGMERVLLLRFDAKLAAM
                     HAEDFVEQVLVRRLGVRQAWVGEGFRFGNKRAGDLALLQQIGKMHGLEAGEVAPFELD
                     GERVSSTRIRALLGVGDFDAAARLLGRRFAIGGHVVRGAQLGRKLGYPTANLRLGSRT
                     APVGGVFAVRVAGIRAQPMPGVASLGTRPTVDGREVLLEAHLFDFDGDLYGKRIEVEF
                     VQNLRDEEKFDSLDAMVKQIDLDAQAAWKILSRSTTATAGAST"
     gene            3551..>4710
                     /locus_tag="VFF05_01135"
     CDS             3551..>4710
                     /locus_tag="VFF05_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZW50440.1"
                     /translation="MSIDYKNTIHLPLTAFAMKADLPKREPGMLADWEKARRYEQIQK
                     HTANREHAFILHDGPPYANGAIHLGHAVNKILKDIVVKSKLMAGFRSPYVPGWDCHGL
                     PIEVAVEKKVGKVGVKVDAAEFRKLCREYAGKQIDLQRADFKRLGVLGEWDNPYRTMD
                     FRYEADIIRALARIVANGHVVRGFKPVHWCFDCGSALAEAEIEYQDKTSPAIDVAYDA
                     VDPKALAAKFGVDAGDAIVSVPIWTTTPWTLPASFAVTLGPELDYVLVEGPQRAGKRV
                     LLVLARALAEKTLGRYGVETPKTLGKAKGEALEGLELKHPFYERQVPVILGDHVSAED
                     GTGAVHTAPGHGAEDFAVGQKYGIVDKYTPTELNPVGANGVYLPDTPIFAGQ"
BASE COUNT          779 a         1455 c         1563 g          913 t
ORIGIN      
        1 tcatcgccga catccccggc ctcatcgaag gcgcggccga tggcgccggc ctcggaatcc
       61 agttcctcaa gcacgtctcg cggacgcggc tgttgctgca tctggtcgac atggccccgc
      121 tggacgatgc ggtcgatcca gtcgaacagg tgcatgcgat cgaggcggag ctcgagaaat
      181 tcgacccgga attactggag cggccgcgct ggctggtctt caacaaggcc gatctgctgg
      241 accctaaaga acgtgacaaa ctcgcgaagg ccatcgtgcg caagctgaaa tggaaggcgc
      301 cgtggttcgt ggtttccgca atcgcgaagg aaggcacctg ggatgtctgc ctgaaaatcc
      361 aggctttctt cgacgctcta cggagcgaac aggcgtgaga cggcgagcaa caaaaaagcc
      421 gacagaagtc ggcttttttg gagaggcgct caatcgctca gagagctttg atatgcgcgg
      481 tcaggcggct cttgtggcgc gaagccttgt tcttgtggat caggccacgg ctggaatagc
      541 ggtccatgac cggctcggca gccttgtagg cagcttcggc gccgggcttg tccttcgcgg
      601 cgatggcgtt gacgactttc ttgatcgccg tgcgcagcat cgagcgcgag ctggcgttgc
      661 gcgcgttgcg cttgaccgcc tggcgggcgc gctttttggc ggatttgatg ttggccaagg
      721 gaaaactccg ttaatcgttg gtgggttgca aaaccgggca tcctgccgcg gtttgcaagc
      781 aaacggtcgc ggccaagccg cgccacattt gccgcaacgc ccgcttgcgc ggtcgctgcg
      841 gcggcacatc cgtgtgccga ggcgcccttg caggctgacc gcggctggaa atgagccggt
      901 aattgtaggc ggcgcgcggg tttgcgtcaa tcaaggcggg gttttcgcgt gaaggcgccc
      961 agtctgctgc gcggggtctt ttcgttcagc gcgatgacca tgatttcgcg cgtgctgggg
     1021 ctggcgcgcg acgtcgcgat cagccacgtc ttcggcgcca atgccgctac cgacgccttc
     1081 gtcatcgcgt tccggattcc caatttcatg cgccggctgt tcgccgaagg ctcattctcc
     1141 acggcctttg tgccggtgtt cacccaagtc aaggaaaccc gcagccatgc cgaacttcgc
     1201 gaactgaccg cgcgcgtggc cggcacgctg ggcggcgcct tgctgatcat taccgcgctg
     1261 gggctgatct tcgcgccgca actggcgatg accttcgcgc cgggcgcgct ggaagaaccc
     1321 ggcaagctcg gcctgacgac cgacctgttg cggctcacgt tcccctacct gctgttcgtc
     1381 tcgttgacag cgctcgccgg cggcgtgctc aacagtttcc accgctttgc gctaccggcg
     1441 ctggcaccgg tcatcctcaa catctgcatg atcgcgggcg cactgtggct ggccccgcac
     1501 ctgcaggttc cgatcctgtc gctgggctgg gccgtgctcg tggccggcgt gctgcaactg
     1561 ttgttcctgc tgccggcgct gcgcggcatg aacctgctaa ccctgccgcg ctggggctgg
     1621 aagcatccgc aagtgcgccg gatcatgcgc ctgatgctgc cgaccctgtt cggttcgtcc
     1681 atcgcacaac tgaacctgct gctcgacacc atcgtcatct cgctgctcgc aaccggctcg
     1741 cagacctgga tgtaccaggc cacgcggttc ctcgaactgc cgctgggcgt gttcggcgtc
     1801 gctctgggca cggtgatcct gccggcgctg tcgcggcacc acgtcgccga agacggcgcg
     1861 ggattttcgc gcgcgctcga ctggggcctg cgcacgacct tgctgatagc ggtcccggcg
     1921 atgtgcgcct tgatgatcct cgcggaaccg ctggtcgcca cgtttttcca gaatggccaa
     1981 tacaccgcgt tcgacacgca catgaccgct atcgccatcg tcgcactcgg tacgggtgtt
     2041 ccggccatcg cgctcacgcg cacattgctg ccggcattct attcgcggca ggacacacaa
     2101 acgccggtgc gcgccggcgt cgccgcgctc gtcgtcaaca tggtgttcaa ttttattttg
     2161 atcgccgcgc tgttcgaggc atgggcggcg ccggacctga agtcggttcc ctggttggac
     2221 ggcatcgcgc gcctgcccgg attgcatttg ggtctggcta tcgcgagttc cgtgtccaac
     2281 tacctgcaat tcgcgctgct gtggcgctac ctgaaacgtg caggtgtcta cacgcgccag
     2341 tccggctgga cgcggcattg gctgcggctc ggcatcgcca gcgcggcgat gatggcggtg
     2401 ctggcggtag gcgtctggtt gtggccggac tggaccaccg tgcgcattgt cacgcgcggc
     2461 tggcatctcg ccgtgctggt cgtcgctgct gcttgcgcct acgttgcggt cttgttcgga
     2521 accggatttc ggatgcgtga tctccacgct gtctgatcgt ttgtgcgccg cagcgtaacc
     2581 gctatactcg caccatgaat tggttgtatc gagacgccgc gggtcagtgc cttgcgccgc
     2641 gcgggagtgc ggtctgcatc ggcgcattcg acggcgtcca tcgcggccat cagtcggtgt
     2701 tggcgcaggt tcgcgcgagc gcgcaggcgc aaggactgac gccggtcgcg gtgagtttcg
     2761 atcccatccc gcgcgtgttt ttcgcgcgcg aaaaaggcgt gccgcagttg agttccacgc
     2821 gcgagaagat cgcgctgctg cggcaagccg gcatggagcg cgtgctgctg ttgcgcttcg
     2881 acgccaagct cgcggcaatg catgcggagg atttcgtcga gcaggtgttg gtgcgccggc
     2941 tcggtgtccg gcaggcgtgg gtgggtgaag gctttcgttt cgggaacaag cgcgcgggcg
     3001 atttggcgct gctgcaacag atcggcaaaa tgcacgggct cgaggcgggc gaagtcgcgc
     3061 cattcgaatt ggatggcgag cgcgtatcga gcacgcgcat ccgcgcgctg ctcggcgtgg
     3121 gcgatttcga cgcggcggca cgcctgctcg gtcgccgctt cgccattggc gggcatgtgg
     3181 tgcgcggcgc gcagcttggc cgcaagctcg gctatccgac tgcgaacctt cgcctgggat
     3241 cgcgcacggc gccggtgggt ggcgtattcg cggtgcgcgt cgccgggatc cgtgcgcagc
     3301 cgatgccggg cgtggcgagc ctgggcacgc gcccgaccgt cgacggccgc gaagtgctgc
     3361 tcgaagcgca tctgttcgac ttcgacggcg acttgtatgg aaaacgtata gaagtggaat
     3421 ttgtgcagaa tttgcgcgac gaggaaaaat tcgacagcct cgacgcgatg gtgaaacaga
     3481 tcgaccttga cgcgcaggca gcgtggaaaa ttttgagccg gagcacgacg gcgacggctg
     3541 gagcatcgac gtgagcatcg attacaagaa cacgatccac cttccgctaa ccgccttcgc
     3601 catgaaggcc gacctgccca agcgcgagcc gggcatgctg gccgattggg aaaaggcgcg
     3661 gcgctacgag cagattcaga agcacaccgc gaatcgcgag cacgccttca tcctgcacga
     3721 cggtccgccg tatgcgaacg gcgcgatcca tctcggccac gcggtcaaca agatcctcaa
     3781 ggacatcgtg gtcaagtcca aactcatggc ggggtttcgt tcgccctatg tgcccggttg
     3841 ggattgccat ggcctgccca tcgaagtagc ggtcgagaaa aaagtcggca aggttggcgt
     3901 gaaggtcgat gcggcggaat tccgcaaact gtgccgcgaa tacgcgggca agcagatcga
     3961 cttgcagcgc gccgatttca agcgcctcgg cgtgctcggc gaatgggaca atccgtatcg
     4021 caccatggat ttccgctacg aagcggacat catccgcgca ctggccagga tcgtcgccaa
     4081 cggccacgtc gtgcgcggct tcaagccggt gcattggtgt ttcgattgcg gttcggcgct
     4141 ggccgaggcc gagatcgagt accaggacaa gacttcgccc gccatcgacg tggcctacga
     4201 cgcggtcgat cccaaggcgc tcgcggcgaa gttcggcgtc gatgccggag acgcgattgt
     4261 ttcggtgccg atctggacga ctacgccgtg gacgctgccg gcgagtttcg ccgtcaccct
     4321 gggaccggaa ctggattacg ttctggtcga aggtccgcaa cgtgccggca agcgcgtact
     4381 gctcgtgctg gcgcgcgcgc tggcggaaaa aacgctcggc cggtacggcg tcgaaacgcc
     4441 gaaaaccttg ggcaaggcca aaggcgaggc actggaaggg ctggaactca agcatccgtt
     4501 ttacgagcgc caggttccgg tcatcctcgg cgatcacgtc tccgccgaag acggcaccgg
     4561 cgccgtgcac accgcgcccg gacacggcgc ggaggacttc gcggtcggcc agaaatacgg
     4621 cattgtggac aaatatactc cgaccgaatt gaatccggtt ggcgcgaacg gtgtgtatct
     4681 gcccgatacg ccgatctttg cggggcagtt
//
LOCUS       DASRVO010000097         1196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_28634, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000097 DASRVO010000000
VERSION     DASRVO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28634"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..753)
                     /locus_tag="VFF05_01140"
     CDS             complement(4..753)
                     /locus_tag="VFF05_01140"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW50441.1"
                     /translation="MFYICIPAFDEAPTIGLLLWRIRKVLEEHPREYELIVLDDGSTD
                     ATADTLKPYAGVLPLTVLRNEQCRGYASAIDSLARTAVKRTRYPRRDAAILMQGDFTD
                     QPEHIPELIKRFEGGADVVIAEQPPSPRVPAAVRRLRLLAPWVVRPFLKVAGVADPFG
                     TFRLFRISVLRDVIRDAGEAPIVQWSGWAANVELLVKTTRIARRVETITLDARYDLRP
                     RASRVRPLAAALDLYRFGWASRRRRATSPST"
BASE COUNT          210 a          377 c          411 g          198 t
ORIGIN      
        1 cgctcatgtc gacggactcg tcgcacggcg gcgccggctt gcccaaccga agcgatagag
       61 atcgagcgcg gccgccagcg ggcggacgcg cgacgcgcgc ggcctcaagt cgtagcgcgc
      121 gtcgagcgtg atcgtttcca cgcggcgcgc aatgcgggtt gtcttgacga gcagctccac
      181 attggcggcc caaccgctcc actgcacgat tggcgcctcg ccagcgtcgc ggatgacatc
      241 gcgcaacacc gaaatgcgga acagccggaa ggtaccgaag ggatcggcga ctccggccac
      301 cttgaggaac ggacgcacga cccagggtgc gagcagtcgc aggcgtcgga ctgcagcggg
      361 cacgcggggc gaaggcggtt gttcggcgat cacgacatcg gcgccgcctt cgaaccgctt
      421 gatcaactcc gggatgtgct ccggttggtc ggtgaagtcg ccctgcatca ggatcgcggc
      481 gtcgcgacgc ggatatcgcg tccgcttcac ggcggtgcgg gccagcgagt cgatcgcgct
      541 cgcgtacccg cggcactgtt cgttgcgcag aacggtgagc ggcaacaccc cggcgtacgg
      601 cttgagcgtg tcggcggtgg cgtcggtgct gccatcgtcg aggacgatca actcgtattc
      661 gcgcggatgc tcctccagca ccttcctgat gcgccagagc agcaacccga tggttggcgc
      721 ttcgtcgaac gccggtatgc agatatagaa catggcctca ttggatcata ccgcgaagcc
      781 ggccgtgcgg ttccctgcgc ggaagttcgc gtcgatgcgt aagatgacgg ccgcaagatg
      841 aacgcgcgtc ttaggaaacg caaaggattg ctttgcgccc gctcacggcg cgggactgcc
      901 gttggcactc ggcgtccgag agtgccgcca cgtttttcac tcggcggaat tgcccgcatc
      961 gtagtaggtc gtcacaagta aggcggcgcg gcccgtgccg tgcgacaggg tgtatgagga
     1021 cacgagaccc atcggagagt tgagatgatt ctcggaaatg ttcgcgcgtc actcacgcgc
     1081 gatgatgcgc agttggcgct tcgactcatc ggacgaacct cgcgcgccgc actcgaggac
     1141 gcggaagacg cgctccgcga gcgcggcgtg gacgcgctgc tggatgatcc gcgcct
//
LOCUS       DASRVO010000098         1484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_158574, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000098 DASRVO010000000
VERSION     DASRVO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158574"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..>1484
                     /locus_tag="VFF05_01145"
     CDS             22..>1484
                     /locus_tag="VFF05_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HZW50442.1"
                     /translation="MRLPLPKRTRIVAAVAAIAFIALLVAGPFASSERGEMLFALTLI
                     LDTALLIAFLSRRPAFALAVPAVVFGGLLIAGALKFQYLTTPLLAPDLVYFLNRDLLD
                     VATRYPSVMTALVGGAVLIPGLLILVWRLDRPHLFAHLHPAPRRLVQGVGALAAVALL
                     VAVDAPRGPFASVFDKGMWAMMNDRNYLVDFFASFWQTQVVIPKPTADADKSLVWTQS
                     SADNPPACAKNRCTATKFASAQEHPDIVSILEESTFDPSMLRDCTIGALCKRKMFEAD
                     GRTRAHGFATMHVWGGGTWTSEFSLLTGLDHLSFGEAGLYAPYNLAPRVAYTLPRVLH
                     AAGYRVIAIYPMTGDFINARNAYKDYGFDKFYDGQDYGLSWESPDSDLVQVFDRIYAQ
                     EKKTIGKQPLFVMMLTLRQHGPHMDPLKSMPAPFDKPLFPGRWKPKDLDDWLNLNLAN
                     YLYRLSGSDAAIAHIEKTLLDSGRPAVLFHFGDHQPS"
BASE COUNT          245 a          501 c          455 g          283 t
ORIGIN      
        1 tccagccgtt atgatgcggc catgagactg ccgctcccga aacgcacgcg catcgtcgcc
       61 gcggttgccg cgattgcatt catcgccctg ctggtcgccg gaccgttcgc gtcgagcgag
      121 cgcggcgaaa tgctgttcgc cctgacgctg atcctcgaca ccgccctgct gattgcgttc
      181 ctgagccggc gtccggcttt cgcgctggcc gtacccgccg tcgtcttcgg cgggctgctg
      241 atcgcgggcg cactgaaatt ccagtacctg accacgcccc tgctcgcacc ggacctggtc
      301 tatttcctca atcgcgatct gctcgacgtg gccacgcgct atcccagcgt gatgacggcg
      361 ttggtcggcg gcgccgtgtt gatcccgggt ttgctgatcc tcgtgtggcg gctggatcga
      421 ccgcacctgt ttgcgcacct gcatccggcc ccacgccggc tcgtgcaagg cgtcggtgcg
      481 ctcgcggccg tggccctgct tgtcgccgtg gatgcgccgc gcggcccctt cgccagcgtg
      541 ttcgacaagg gcatgtgggc gatgatgaac gaccgcaact acctcgtcga tttcttcgcc
      601 tcgttctggc agacgcaggt cgtgattccg aagccgactg cagacgccga caagtcgctg
      661 gtctggacgc aatcgtccgc ggacaatccg cccgcgtgtg cgaagaaccg ttgcacggcg
      721 acgaaattcg ccagcgcgca ggaacatccg gacatcgttt ccatcctcga agagagcacg
      781 ttcgatccgt ccatgctcag ggactgcacg atcggggcgt tgtgcaagcg caagatgttc
      841 gaggccgacg gccgcacgcg cgcgcacgga ttcgcgacga tgcacgtctg gggtggcggc
      901 acctggacca gtgaattctc cctgctcacg gggctggatc acctgagctt cggcgaggcc
      961 gggctgtacg cgccgtacaa cctcgcgccg cgcgtcgcct acacgctgcc gcgcgtgctg
     1021 cacgcagccg gctaccgtgt catcgcgatc tacccgatga ccggcgactt catcaacgca
     1081 cgcaacgcct acaaggacta cggcttcgac aagttctacg acggccagga ctacggcctg
     1141 tcgtgggagt cgccggacag cgacctcgtg caggtcttcg atcgcatcta tgcgcaggaa
     1201 aagaaaacca tcggcaagca gccgctgttc gtgatgatgc tgaccctgcg ccagcatggt
     1261 ccgcacatgg atccgttgaa atccatgccg gcgccgttcg acaagccgct gtttcccgga
     1321 cgctggaaac cgaaggatct ggacgactgg ctgaacctga atctcgccaa ttacctgtac
     1381 cggctgtccg gatcggacgc ggcgatcgcg catatcgaga aaactctgct cgacagcggg
     1441 cggcccgccg tgttgttcca ctttggcgat caccagccgt cgtt
//
LOCUS       DASRVO010000099         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_54892, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000099 DASRVO010000000
VERSION     DASRVO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54892"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..974)
                     /locus_tag="VFF05_01150"
                     /pseudo
     CDS             complement(1..974)
                     /locus_tag="VFF05_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010378222.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(1035..1511)
                     /locus_tag="VFF05_01155"
     CDS             complement(1035..1511)
                     /locus_tag="VFF05_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50443.1"
                     /translation="MPALLRTFLRTLLLASTLVLLAACGQVKHPINPPGASIQQLDVL
                     ADGRWKLQVRLENFSDQTVRYGAFKAELRVGGEAAGAIDTNADMDIPGENADILDITL
                     VPGADAAKAFKADLSRPDGVAYELKGTIQIPATGKDYKFAHKSRLSPIPGVANSYR"
     gene            complement(1587..>1867)
                     /locus_tag="VFF05_01160"
     CDS             complement(1587..>1867)
                     /locus_tag="VFF05_01160"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HZW50444.1"
                     /translation="EHFKGNPDDPIAWGVSTYNRFYNWIWLYNGFHAEHHFRPKVHWT
                     KMVQLRDSIRDEQRAHGVHVMNHCHGLGFLDRKSPQYTQVERPAANAA"
BASE COUNT          305 a          565 c          619 g          378 t
ORIGIN      
        1 tgcaggcgtt cgcgcgcata ggcgagcgca ccctgatagg cgcgttcgat caggcccaaa
       61 ccttatatgc cgacggcgag gcgcgcggtg ttcatcatgg tgaacatcgc gttcaggccc
      121 ttgttcggcg cgccgatcag gtaaccttcg gcgccgtcga agttcatcac gcaggtcacc
      181 gaggcgtgga tgcccatctt gtgctcgata gacccgcagc gtaccgcgtt gcgcgcgccc
      241 atttgtccac ttttgtccac tttatgcttc ggcacgatga acagcgaaat gcccttcacg
      301 ccggcaggcg catcgggcag gcgcgcgagc accaggtgca cgatgttctc ggtcagatcg
      361 tgctcgccgg cggtgatgaa gatcttggtg ccggtaacgc ggtaggttcc atctgcattg
      421 gggtctgcac gcgtcttgag caggcccaag tccgatccgc agtgcggctc ggtcaggcac
      481 atggtgccgg tccagcggcc ttcgacaatg ggcttgagga aggccacgcg ctgccactcc
      541 tcgccgtgca ggcgcagcgc ctcggtggcg ccgtgcgaga gtagcgggaa attgccccac
      601 gacaggttcg ccgaatcgag catttccttg agtggcgcgc cgatggattc gggcagatgc
      661 tggccgccgt aggcttccgg tgccgtcaag ccgctccagc cgccatcgac atactttgcg
      721 taggcatcct tgaatcccgc tggtgtcgtc accgcgccgg tggccttgtc cagtgcgcag
      781 ccttgttcgt cgcccggcgc gttaagcggc gcgagcacgg tttcggaaaa cctcgccgcc
      841 tcgtcgagga tcgcatcgac cacgtcgcgc gaggcgctgg cgccaccatc gatgcgtgcg
      901 tattgcgcgt cgagcttgag cagatcgaac atgacgaagc gcatgtccgc gagcggggct
      961 ttgtatgtgg tcatggcaat cccttttaat cggcgtcatc ccggcattcc ctgccgggac
     1021 gacagtggtg tttcttaacg gtacgagttg gccacgccgg gaattggcga cagccggctc
     1081 ttgtgcgcga atttgtaatc tttgccggtg gcgggaatct ggatggttcc cttcagctcg
     1141 taggccacgc cgtccgggcg cgacaggtcg gccttgaatg cttttgccgc gtcggcgccg
     1201 ggcaccagcg tgatgtcgag gatgtcggcg ttttcgccgg gaatgtccat gtccgcattc
     1261 gtgtcgatgg caccggccgc ttcgccgccg acgcgcagtt ccgccttgaa tgcgccgtag
     1321 cgaacggtct ggtcgctgaa attttccagt cggacctgca atttccagcg cccgtccgcc
     1381 agcacgtcga gttgctggat gctggcgccg ggcggattga tcggatgctt gacctgcccg
     1441 caggcagcga gcagcacgag cgtcgatgcg agcaggaggg tgcgcaggaa cgtacgcaac
     1501 aaagcgggca tggccgtcac cgttgtgagc gaaggcctac cttagccaaa aggcggacgc
     1561 ggtggaagcg gcgcgatgcc gctggttcac gcggcgttcg cggcgggacg ctcgacctgc
     1621 gtatactgcg gcgacttgcg gtcaaggaag ccgaggccgt ggcagtggtt catcacgtgc
     1681 acgccgtgcg cgcgctgctc atcgcggatc gaatcgcgta actgcaccat cttcgtccag
     1741 tgcactttcg gacggaagtg gtgctcggcg tggaaaccgt tgtagagcca gatccagttg
     1801 tagaaccggt tgtaggtgct cacgccccag gcgatggggt cgtccggatt gcccttgaaa
     1861 tgttcgt
//
LOCUS       DASRVO010000100         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_288557, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000100 DASRVO010000000
VERSION     DASRVO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288557"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1037)
                     /locus_tag="VFF05_01165"
     CDS             complement(<1..>1037)
                     /locus_tag="VFF05_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZW50445.1"
                     /translation="VAEWNGEKVTVWTGTQRPFAVRDELVQAFRLSPAQARVIMPDTG
                     SGYGGKHTGDAAVEAARLAKVAAVPVKLVWTREEEFTWAYFRPAGVIDVRSGVQKDGT
                     IVAWSFHNYNSGPAGIEPQYEIANKDVQFHPVKSPLRQGSYRALAATANNFARESHID
                     ELANAVGMDPLDFRLKNLTNERVRAVLEAAARQFRWKTFRSTAEAGLGMACGTEKGGF
                     YAACVEVQVDRQVQIRRVVIAFECGAVVNPDGLRNQVSGAIVQGIGGALFEAIQFADN
                     KILNPHMNDYPLPRFRDVPQIETVILNRKDLPSAGAGEAPICALAPAVANAIFAATGT
                     RVRHMPLAPAGT"
BASE COUNT          164 a          354 c          329 g          190 t
ORIGIN      
        1 gttcccgcgg gcgccagcgg catgtggcgc acgcgcgttc cggtggcggc gaagatcgcg
       61 tttgccaccg cgggcgcgag cgcgcagatg ggcgcttcgc ccgctcccgc cgacggcagg
      121 tccttgcgat tgaggatcac ggtttcgatc tgcggcacgt cgcgaaaacg cggcagcggg
      181 tagtcgttca tgtggggatt gagaatcttg ttgtcggcga actggatcgc ctcgaacaac
      241 gctcctccga ttccctgcac gatcgcgccc gacacctggt tgcgcaggcc atcgggattc
      301 accaccgcgc cgcattcgaa ggcgatcacc acgcgccgga tctgcacttg gcggtctacc
      361 tggacttcca cgcaggccgc gtagaatccg cccttctcgg tgccgcacgc cattccgagg
      421 ccggcttcgg cggtgctccg gaacgtcttc caccggaact gccgggccgc cgcctccagc
      481 accgcgcgca cgcgctcgtt ggtcaggttc ttgaggcgga agtcgagtgg gtccatgccg
      541 accgcgtttg ccagttcgtc gatgtgcgac tcgcgcgcga agttgttggc ggtcgcggcc
      601 agcgcccgat acgagccctg gcgcagcggc gacttcaccg gatggaactg cacgtccttg
      661 ttcgcaatct cgtactgcgg ctcaattccg gccggcccgg aattatagtt gtggaagctc
      721 caggcaacga tcgtgccgtc cttctgcacg ccgctacgca catcaatgac gcccgccgga
      781 cggaaatacg cccaagtaaa ctcttcctcg cgcgtccaga ccagcttgac gggaaccgcg
      841 gcgactttcg cgagacgcgc tgcttccaca gccgcgtcgc cagtgtgctt gccgccatag
      901 ccggaaccag tgtcgggcat gatgacgcgc gcttgcgcgg gggagagccg aaacgcttgt
      961 accagttcat cgcgcacggc gaacggacgc tgcgtgccgg tccacaccgt gaccttttcg
     1021 ccgttccatt cggcgac
//
LOCUS       DASRVO010000101         1621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_340632, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000101 DASRVO010000000
VERSION     DASRVO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340632"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VFF05_01170"
     CDS             <1..566
                     /locus_tag="VFF05_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW50446.1"
                     /translation="IGYEPYLSGIGLNAECPNLLIQNRLNATLDRARQETGRKVHLIG
                     HSLGGIMARSVANRRPEDVASVITLAAPFRGTVAHGSILRAAESVRRQIHREHGPGVL
                     PQCYTAHCTCDFVNYLRSALPESIHQTAIYTRDDGLVDWRFCRTGRSESDCEVPGTHI
                     GLVFNATVYRTIAERLAAAECEQHVTG"
     gene            complement(556..>1621)
                     /locus_tag="VFF05_01175"
     CDS             complement(556..>1621)
                     /locus_tag="VFF05_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HZW50447.1"
                     /translation="SEATVSAILPFTWKNPAISRRERGVFKVLKPHVPAFYAEDMDLL
                     QGLADFLKQNHARYGVGARGLADTFREVRRLLQHELDLRGEQRQLVRAAATYSGVRDV
                     RVPRIILPLCTASMTAMSEERGMKITRAVRRMSKTERRRVAQQIIQALIATPMFASSD
                     DIVFHADPHAGNLLYDRRTRQVVILDWALVGDLSLEQRRHLMLLFLMVLLRDPVGIFD
                     EVLALNLQRPVPKARRQAVRQAIDKFLDELPVSRMPDSMDAMSLLQKLAFESLRLPAS
                     LILLRKVMFTLEGILQDVVGTDFAFDRILVRSLFWRWLSRKLPLGAPLGVSDWFRLQS
                     STLLSATRWCLYAAQAAANR"
BASE COUNT          306 a          520 c          539 g          256 t
ORIGIN      
        1 ggatcgggta cgagccttac ctgtcgggca tcgggctgaa cgccgagtgt ccgaacctgc
       61 tcatccagaa ccggctgaac gcaacactgg accgcgcccg ccaggaaacg ggccgcaagg
      121 tgcacctgat cgggcacagc ctgggcggga tcatggcgcg ttcggtggcg aaccgcaggc
      181 cggaagatgt cgcgtcggtc atcaccctgg ccgcgccttt ccgaggaacg gtggcgcacg
      241 gcagcattct gcgggcggcc gagagcgtgc ggcgccagat tcatcgcgaa catggaccgg
      301 gagtgttgcc gcagtgctac acggcgcact gcacctgcga tttcgtgaat tacctgcgct
      361 cggcgctgcc cgaatcgatt caccagaccg cgatttacac gcgcgacgac gggctcgtcg
      421 actggcgctt ctgccgcacc ggcaggagcg aaagcgattg cgaagtccca ggcacgcaca
      481 tcgggctcgt cttcaacgcc accgtctacc ggacgatcgc ggagaggctg gcggcagcgg
      541 agtgcgagca acacgttacc ggttagcggc cgcctgcgcc gcgtagagac accagcgcgt
      601 cgcgctcaag agcgtgctgg actgcaaccg gaaccaatcc gacaccccga gcggagcgcc
      661 gagcggcaac ttgcgcgata gccagcgcca gaacagcgat cgcaccagga tgcggtcgaa
      721 ggcgaaatcc gtccccacca cgtcctgcag aatgccctcc agcgtgaaca tcaccttgcg
      781 caacaggatc agcgaagccg gcaaacgcag gctctcaaag gccaatttct gcaacaggct
      841 catggcgtcc atgctgtcgg gcatgcgtga gacgggcagt tcgtcgagga acttgtcgat
      901 cgcctggcgc accgcttggc gtcgcgcttt gggaaccggc cgctgaaggt tcagggcaag
      961 cacctcgtcg aagattccga ccgggtcgcg cagcaggacc atcaggaaca gcagcatcaa
     1021 gtgccgtcgc tgttcgagcg ataagtctcc caccagcgcc cagtcgagaa ttaccacctg
     1081 gcgggtacgg cggtcgtaga gcaggttgcc ggcgtgcgga tcggcgtgga agacgatatc
     1141 gtcggaggac gcgaacatcg gggtcgcgat cagcgcctgg atgatctgtt gcgccacccg
     1201 ccggcgctcc gttttcgaca tgcggcgcac ggcgcgcgtg attttcatgc cgcgctcttc
     1261 gctcatcgcc gtcatggatg cggtgcagag cggcaggatg atccgcggga cgcgcacgtc
     1321 acgcacgccg ctgtaagtgg cggccgcgcg gacgagttgg cgctgctcgc cgcgcaggtc
     1381 cagctcatgt tgcagcaggc ggcgaacttc gcggaaggtg tcggccaagc cgcgcgcgcc
     1441 gacgccgtag cgcgcgtggt tctgcttcag gaaatccgcc agcccctgca acaagtccat
     1501 gtcttccgcg tagaaggcgg gaacgtgcgg cttgagcacc ttgaagacgc cgcgctcgcg
     1561 ccgcgagatc gctggatttt tccacgtgaa gggaagaatc gcgctgacgg tcgcttccga
     1621 g
//
LOCUS       DASRVO010000102         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_54959, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000102 DASRVO010000000
VERSION     DASRVO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54959"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1014
                     /locus_tag="VFF05_01180"
     CDS             91..1014
                     /locus_tag="VFF05_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HZW50448.1"
                     /translation="MEKLKILIDTDPGVDDALAILMAHRHADVVGLTIAAGNVGLAHT
                     TANALKLMEIIGAATPVFPGCATPLVHAAEDAAFVHGLDGFGDTGYSPSARRAENESA
                     AQAILRLSHEYAGKLVIVAMAPLTNIALALRLDPELPSRIARLVVMGGAVTGHGNTTV
                     SAEFNIGFDPEAAHIVFSAWPQFELADWELTVRHGIAFDTIARWVGADNPTARFFDAI
                     SRKTRAWSRERGRPKLALADPLAMAVVLQPDIVASAATHHVAIALDGATRGATVVDWD
                     NRSGKAPNARIVLDVDQVRFEALAASALGAG"
     gene            complement(966..>1509)
                     /locus_tag="VFF05_01185"
     CDS             complement(966..>1509)
                     /locus_tag="VFF05_01185"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HZW50449.1"
                     /translation="VLVERPFRIGDIVSIGEVEGTVARIRTRATTIVDWDNKEVIVPN
                     KSFITERLTNWSLSNTVTRVVLRIGAAYASDPRQVRELLLEIARAEPLVVDDPAPSCW
                     FVQISACTFDFDLRVFIADIGDRNRARDALYMRIAEVFREKNIEMAFPQTDIWFRNAM
                     PLTPAQPAPSADAASASKRT"
BASE COUNT          256 a          461 c          520 g          272 t
ORIGIN      
        1 gaatcggccg tggcgagcac gacatactgg ggatacgcac gagcggtcat cctggcgcga
       61 cgctgaacgt aggtaacttg gagtttagaa gtggaaaagc tgaaaatcct gatcgacacc
      121 gatcccggcg tggacgacgc gctggcgatc ttgatggcgc atcgccacgc cgacgttgtc
      181 ggcctgacca tcgccgccgg caatgttgga ttggcgcaca cgacggcgaa cgcgctgaaa
      241 cttatggaaa tcatcggcgc ggcgacaccg gtgtttcccg gctgcgcgac gccgctcgtg
      301 catgcggcgg aagatgccgc gttcgtgcac gggctcgacg gtttcggcga taccggttat
      361 tcgccgagcg cgcgtcgtgc ggaaaacgaa tcggcggcgc aggcgatttt gcgcctgtcg
      421 cacgaatacg cgggaaagct ggtcatcgtc gcgatggcac cgctgaccaa catcgcgctg
      481 gcgttgcggc tcgatccgga attgccttcg cgcattgcac gtctcgtcgt catgggcggc
      541 gcggtgaccg ggcacggcaa cacgacagtt tccgcggagt tcaacatcgg tttcgatccg
      601 gaagccgcgc acatcgtgtt ctcggcatgg ccgcagttcg aattggccga ttgggaattg
      661 accgtacgcc atggcatcgc gttcgatacg atcgcgcgct gggtaggtgc ggacaatccg
      721 accgcgcggt ttttcgacgc gatctcgcgc aagacgcgcg cctggtcgcg cgagcgcggg
      781 cgccccaagc tcgcgctcgc cgatccgctg gcgatggcgg tggtgctgca gccggatatc
      841 gtggcgagcg ccgccacgca tcatgtcgcc atcgcgctcg acggcgcgac gcgcggcgcg
      901 accgtggtcg attgggacaa tcgttcgggc aaggcaccga acgcgcgcat cgtattggac
      961 gtggatcagg tgcgcttcga ggcgcttgcg gcgtccgcgc ttggcgctgg ctgagcgggt
     1021 gtcagcggca tcgcgttgcg gaaccagatg tcggtttgcg ggaacgccat ctcgatgttc
     1081 ttctcgcgga acacttcggc gatgcgcatg taaagcgcat cgcgcgcgcg gttgcggtcg
     1141 ccgatgtcgg cgatgaacac gcgcaggtcg aagtcgaacg tgcaggcgga aatctgcacg
     1201 aaccagcaac tcggcgccgg atcgtccacc accagcggct cggcacgcgc gatctcgagc
     1261 agcagttcgc gcacctggcg cggatcgctg gcgtaggcgg cgccgatgcg cagcaccacg
     1321 cgcgtgaccg tgttcgacag cgaccagttg gtcaggcgct cggtgatgaa actcttgttc
     1381 ggcacgatca cttccttgtt gtcccagtcg acgatcgtgg tggcgcgcgt gcggatgcgg
     1441 gcgacggtgc cttcgacttc accgatgctg acgatgtcgc cgatgcggaa cgggcgttcg
     1501 accagcacc
//
LOCUS       DASRVO010000103         1097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_54983, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000103 DASRVO010000000
VERSION     DASRVO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54983"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFF05_01190"
     CDS             complement(<1..235)
                     /locus_tag="VFF05_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZW50450.1"
                     /translation="MQPFLISAASVALAEFGDKTQLLALVLAAKYRRPWLIALGILLA
                     TLANHALAGAFGALVAHWFAPQTLRWLVAASFFA"
     gene            complement(355..900)
                     /locus_tag="VFF05_01195"
     CDS             complement(355..900)
                     /locus_tag="VFF05_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HZW50451.1"
                     /translation="MSATDAKIIVEFWRDAGYRRWFTRDAEFDARFSARFLSSHHAAA
                     RREFEHWIGDAHGALALMILLDQLPRNAFRDSAHAYATDSLARHYAGRAIDAGLDHNI
                     EASLRAFIYLPFEHSEAHADQDRSVALFRALGEAEYSKYAEAHREVILRFGRFPHRNR
                     ALGRVSTPEEKAWLDAGGGFG"
BASE COUNT          207 a          342 c          367 g          181 t
ORIGIN      
        1 cggcgaagaa actcgccgcg accagccagc gcagggtttg cggcgcgaac caatgcgcca
       61 ccagcgcgcc gaacgcaccg gccagcgcgt gattggccag cgtcgccagc aaaatcccga
      121 gcgcgatcaa ccagggccga cggtatttcg ccgccagcac cagcgcgagc agttgcgtct
      181 tgtcgccgaa ttcggccagc gcgacgctgg cggcggagat gaggaagggt tgcatggcgg
      241 gttgcgtgga aaacgcacag gatagtgcac attcgacacg ttcatcgcga ggtcgcactg
      301 cgactgacca cggacgagca gtctgtcggc tcaagccgcc gtctgcggtc cgaatcaacc
      361 aaatccgccg ccggcgtcga gccacgcctt ttcttccggc gtcgagacgc ggccgagtgc
      421 tcggttgcgg tgcgggaagc gcccgaagcg caggatgacc tcgcgatgcg cctcggcgta
      481 cttgctgtac tccgcctcgc cgagcgcgcg gaacagcgcg accgagcggt cctggtccgc
      541 gtgcgcttcg gagtgctcga acggcaggta gatgaaggca cgcaatgacg cctcgatatt
      601 gtggtcgagt ccggcatcga tcgcacgccc ggcatagtgg cgcgccaagc tgtcggtcgc
      661 gtaggcgtgc gcgctgtcgc ggaatgcgtt tcgcggcaac tgatcgagca ggatcatcaa
      721 tgccagcgcg ccatgggcat cgccgatcca gtgttcgaat tcgcgtcgcg cggcagcgtg
      781 gtgtgagctc aggaagcgtg cgctgaaccg cgcatcgaat tcagcgtcgc gcgtgaacca
      841 gcgccggtag ccagcgtcgc gccagaactc gacaatgatt tttgcgtcgg tggcagacat
      901 ggattacgcg ttgtcgatct tagtcggagt aaatgcccgc cgccgcatcg gcaaaaatcc
      961 tttcaggcga agcgaaggag tccggcgcat acccatggcg acgcttactg ccattcctgc
     1021 ggctgcggac aaacgtgctg gaaatcatcg gcgttcgcga gccggccgtg aaaccggttg
     1081 gcgaagacgt cctgggc
//
LOCUS       DASRVO010000104         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_28827, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000104 DASRVO010000000
VERSION     DASRVO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28827"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFF05_01200"
     CDS             complement(<1..282)
                     /locus_tag="VFF05_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZW50452.1"
                     /translation="MATTEVKVPDIGNYTNIPVIEVLVKPGDTVTKDQGLVTLESDKA
                     TMEVPSTVAGVVKEVKVKLGDEVSEGTIVAIIEAAGAAAAPVAKAETSKP"
     gene            complement(295..924)
                     /locus_tag="VFF05_01205"
     CDS             complement(295..924)
                     /locus_tag="VFF05_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50453.1"
                     /translation="MAMDEFTDIVIVDLDVNRTTWSRVHSSMRTLYLKLDREPNPEWT
                     RLFYEERNSRVELKRHGLWIEDGYIVFDCLLIDVDDYHLPDIRQSIDYANARSRVLVE
                     VRHAEAERLRSEARSEAHMLGALRASIRSPATAPSEMPAATPEPLAEPAKIVEPVATA
                     TAAAESAPEPESTPPDTQIEPLHVEREEALEAKRNDWRARFRAALHRNK"
     gene            complement(927..>2030)
                     /locus_tag="VFF05_01210"
     CDS             complement(927..>2030)
                     /locus_tag="VFF05_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HZW50454.1"
                     /translation="SEAAAKPAPAPAPAAAPPKTEMPAPAPVSQATSVPKAPSTPATA
                     AAMPSSPPIPFGADTVMPGKVPLASPVVRMIARELGVDLAQVTGSERNGRITKQDVRA
                     HVKRILEGGGPVRAAGAGGGLNLLPWPSVDFSKFGEIETRPLSKIKKISGANLARNWA
                     MIPHVTQHDDADITDLEALRVALNKEHEKAGIKFTMLAFIIKAVAAALKKYPDFNASL
                     DASGENLILKKYFHVGFAADTPNGLVVPVLKNADQKGLVEIVQETSELAKKARDGKLG
                     PADMSGGCFSISSLGGIGGTAFTPIVNAPEVAILGVSKSSIKPVWDGKAFAPRLLLPL
                     SLSYDHRVIDGAAAARFTAFLAQLLADMRRSLL"
BASE COUNT          319 a          635 c          670 g          406 t
ORIGIN      
        1 cggcttggac gtctccgcct tggcgaccgg tgccgcagcc gcgcctgcgg cctcgatgat
       61 cgccacgatc gtgccttcgg aaacctcgtc accgagcttc actttcactt ccttgaccac
      121 accggcaacc gtggacggca cttccatcgt cgccttgtcc gattccagcg tgaccaggcc
      181 ctgatccttg gtcaccgtgt cgccgggttt gaccaacact tcgatcaccg gaatgtttgt
      241 gtaattgccg atatccggaa ccttgacttc cgtcgtcgcc acggcgtgct cccgttattt
      301 gttgcggtgt aacgccgcgc gaaagcgcgc gcgccagtcg ttgcgtttgg cctcgagcgc
      361 ttcttcgcgc tcgacatgca gaggctcgat ctgcgtgtcc ggtggcgtgg attccggttc
      421 cggtgcggat tcggcggcag cagtggccgt cgcgaccggt tccacgatct tggccggttc
      481 tgcaagcggc tccggtgtgg ctgccggcat ttccgatggc gctgtggcgg gcgaacggat
      541 ggatgcgcgc agtgcgccga gcatgtgcgc ttcgctgcgc gcttcgctgc gcaggcgctc
      601 ggcctcggcg tggcgcactt cgacgagcac gcgtgaacgc gcattcgcat agtcgatcga
      661 ctggcggata tcgggcaggt ggtaatcgtc gacgtcgatg agcaggcagt cgaagacgat
      721 gtagccgtct tcgatccaaa ggccgtggcg cttgagctcc acgcgcgaat tgcgctcctc
      781 gtagaacaac cgcgtccact ccggattcgg ctcgcggtcg agcttcagat acaacgtgcg
      841 catggacgaa tgcacgcgcg accaggtcgt gcggttcacg tccagatcga cgatgacgat
      901 gtcggtgaat tcgtccatcg ccatggtcag agcaggctgc ggcgcatgtc cgcaagcagt
      961 tgcgcaagga acgcggtaaa gcgcgccgcg gccgcgccgt cgatgacgcg atgatcgtag
     1021 ctcagcgaca acggcagcag caggcgcggc gcgaacgcct tgccgtccca caccggtttg
     1081 atcgaagact tggacacgcc caggatcgcg acttccggcg cgttgacgat cggcgtgaac
     1141 gccgtgccgc cgatgccgcc gagcgaggaa atcgagaagc atccgccgga catgtcggcg
     1201 gggccgagct tgccgtcgcg cgccttcttc gccagctcgc tggtttcctg cacgatctcg
     1261 acgagtcctt tctggtcggc gtttttcagt accggcacga ccaggccgtt cggcgtgtct
     1321 gcagcgaaac cgacatggaa gtatttcttc aggatcaggt tttcgccgga cgcatcgagc
     1381 gaggcgttga aatccggata tttcttcagc gccgcggcca ccgccttgat gatgaaggcg
     1441 agcatggtga acttgattcc cgctttctcg tgttccttgt tcaacgcgac gcgcagcgct
     1501 tccagatcgg tgatgtcggc atcgtcgtgc tgggtgacgt gcgggatcat cgcccagttg
     1561 cgggcgagat tggcgccgga aatcttcttg atcttggaca gcgggcgcgt ttcgatttcg
     1621 ccgaacttgg agaagtccac actgggccac ggcagcaggt tcaagccacc gcctgcgcct
     1681 gcggcacgaa caggaccgcc gccttcgagg atgcgtttga catgggcgcg cacgtcctgc
     1741 ttggtgatgc ggccattgcg ctcgctgccg gtgacctggg ccaggtccac acccagctcg
     1801 cgcgcgatca tgcgcaccac gggactcgcc agcggcactt tgccgggcat gaccgtgtcg
     1861 gcaccgaagg ggatcggagg cgaactcggc atggccgctg cggttgcagg ggtggacggc
     1921 gccttgggca ccgacgtcgc ttgtgaaacc ggcgcgggtg caggcatctc ggttttcggt
     1981 ggggccgcag ccggtgcggg ggcaggcgca ggtttcgccg ccgcttcgct
//
LOCUS       DASRVO010000105         1869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_106567, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000105 DASRVO010000000
VERSION     DASRVO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106567"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /gene="ntrC"
                     /locus_tag="VFF05_01215"
     CDS             <1..1344
                     /gene="ntrC"
                     /locus_tag="VFF05_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HZW50455.1"
                     /translation="FVLGEALREDGVTVREFANAEDLLAALNQDTPDVALSDVRMPGA
                     SGLKLLDEIKARGLHLPVIVMSAFTDVASTAAAYRNGAFDYLPKPFDLDQVRAAVERA
                     LAQNRPPPPPLPATEAEAELIGGAPAMRELFRSIGRVAATNLNVLITGETGTGKELVA
                     RALHRHSARATRPFVAMNTAAIPAELLESELFGHEAGAFTGALKRHPGRFEQAEGGTL
                     FLDEIGDMPLALQMRLLRVLAQGEFYRVGGRELIRADVRVIAATHQELDAKVETGQFR
                     ADLRHRLDVVRLHLPTLSERREDIPLLAARFLARAASDMRLDAKTFSPAALGALAQMP
                     WPGNVRQLENLCRRLAVMASGREIRVSDLPETDEAAASKSADWRAPLRSAVRERLARG
                     ERNIYAQLRSGFDQALLEAALAENGNQRGRAAQQLGLGRNTLTRKLGSSRKRGKR"
     gene            1341..1841
                     /locus_tag="VFF05_01220"
     CDS             1341..1841
                     /locus_tag="VFF05_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZW50456.1"
                     /translation="MTDVAEVRVRAAHAGDCEVLAAFNAAMAVETENKTLDPQALRAG
                     VARVLAEPARGFYLVAEIDGVVVGCLMVTFEWSDWRNGDWWWLQSVYVEPDHRRHGVF
                     RALYAEVERRAVEHADVVGIRLYVERDNARAQRTYASLGMHEDHYRMYAKPLRKLTGS
                     AIDGDA"
BASE COUNT          318 a          587 c          652 g          312 t
ORIGIN      
        1 ttcgtgcttg gcgaagccct gcgcgaggat ggggtcacgg tgcgtgagtt cgccaacgcc
       61 gaggatttgc ttgccgcgct gaatcaagac acgccggacg tggcgttgag cgacgtgcgc
      121 atgcccggcg cgagcggcct gaagttgctg gacgagatca aggcacgcgg cctgcatctg
      181 ccggtgatcg tgatgagtgc attcaccgac gtggcctcca ccgccgccgc gtatcgcaat
      241 ggcgcgttcg actacctgcc caaacctttc gatctggatc aggtgcgcgc ggcggtcgaa
      301 cgcgcgctgg cgcagaaccg gccgccgccg ccgccgttgc cggcgaccga agcggaagcc
      361 gaattgatcg gcggcgcgcc ggcgatgcgc gagttgtttc gcagcatcgg tcgggtcgcg
      421 gcgaccaatc tcaacgtact gatcaccggc gaaaccggca ccggcaagga gctggtcgcg
      481 cgcgccttgc accggcacag tgcgcgcgcg acgcggccgt tcgtggcgat gaatacggca
      541 gcgattccgg ccgaactgct ggaatccgaa ctgttcggcc acgaagctgg cgcgttcacc
      601 ggcgcgctca agcgccatcc gggccgcttc gaacaggccg aaggcggcac gttgttcctc
      661 gacgaaatcg gcgacatgcc gctggctttg cagatgcgcc tgttgcgcgt gctcgcgcag
      721 ggcgagtttt atcgcgtcgg cggacgcgag ctgatccgcg ccgacgtgcg cgtgatcgcg
      781 gccacgcacc aggagctcga cgcgaaagtc gaaaccgggc aattccgcgc cgatttgcgg
      841 caccgcctcg atgtcgtgcg cctgcacctg ccgacgttga gcgagcgccg cgaggatatt
      901 cccctgctcg ccgcacgctt cctcgcccgc gccgccagcg acatgcgcct ggacgcgaaa
      961 acgttttcgc cagccgcgct tggcgcactg gcgcagatgc cttggccggg caatgtgcgg
     1021 caattggaaa acctgtgccg gcgcctggcg gtgatggcgt cgggacgcga gattcgcgtt
     1081 tccgatttgc cggaaaccga cgaagcggca gcttcgaagt cggcggattg gcgtgcgccg
     1141 ttgcgatccg cggtgcgcga gcgtcttgcg cgcggcgagc gaaatatcta tgcacaattg
     1201 cgcagtgggt tcgatcaggc cctgctcgaa gcggcgctcg cggaaaacgg caaccagcgt
     1261 ggccgggcgg cgcagcaact cggcctggga cgcaacacgc tcacgcgcaa attgggcagt
     1321 tcacgcaaac gaggcaaacg atgaccgatg tcgctgaggt tcgggtgcgc gccgcgcatg
     1381 cgggcgattg cgaagtcctg gccgcgttca atgcggcaat ggccgtcgag acggaaaaca
     1441 agacgctcga tccgcaagcg ctgcgcgccg gtgtcgcgag ggtactcgcg gagccggcac
     1501 gcggatttta tctggttgcg gaaatcgacg gcgtggtcgt cggttgcctg atggtcacgt
     1561 tcgagtggag cgactggcgc aatggcgact ggtggtggct gcaaagcgtc tacgtcgagc
     1621 cggaccatcg ccggcacggt gtattccgcg cgctgtatgc ggaagtcgag cgccgtgcgg
     1681 tcgaacatgc cgatgtcgtc ggcattcgtc tgtatgtcga acgcgacaac gcgcgcgcgc
     1741 agcgcaccta cgcatcgctg ggcatgcacg aagatcatta ccggatgtat gccaagccgc
     1801 tgcgcaagct cactgggagc gccatcgatg gcgatgcttg agcgcttgcg cgcgcagctc
     1861 cgggacgcg
//
LOCUS       DASRVO010000106         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_236214, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000106 DASRVO010000000
VERSION     DASRVO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236214"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..580
                     /locus_tag="VFF05_01225"
     CDS             5..580
                     /locus_tag="VFF05_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HZW50457.1"
                     /translation="MTLVERIDALLPQTQCTRCGYPACMDYANAIAGGEANINQCPPG
                     GTAGIIALASLLARAPLPLNPKNGVEKPREIALIDEEVCIGCTKCIQACPVDAIVGAS
                     KLMHTVIADECTGCELCIAPCPVDCIALIPASIPADPGHYRARYIAHTRREELRNAER
                     AAELAARKSRVNAGDAVAAALARARAKKAGA"
     gene            577..1209
                     /gene="nth"
                     /locus_tag="VFF05_01230"
     CDS             577..1209
                     /gene="nth"
                     /locus_tag="VFF05_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZW50458.1"
                     /translation="MNAAKRAEIFRRLREANPKPKTELEYATPFQLLIAVMLSAQATD
                     VGVNKATRRLFPVAGTALAMLALGEEKLKTSISTIGLFNTKAKNVIATCAILVEQFDG
                     EVPASREALEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAPGKTVRAVE
                     DKLEKVVPDEFKRDAHHWLILHGRYVCKARKPDCPNCIIRELCEYRHKTR"
     gene            complement(1266..>1522)
                     /locus_tag="VFF05_01235"
     CDS             complement(1266..>1522)
                     /locus_tag="VFF05_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZW50459.1"
                     /translation="SSDGYSAWGQFKFAPDYSVFARYDHANQSKDINPQLKDTYYNAG
                     LEWNARKGVRLAAVLKHEKLADAVNTTKSNEIGVWAEIAF"
BASE COUNT          305 a          502 c          471 g          244 t
ORIGIN      
        1 ccacatgacg ctcgtcgaac gcatcgacgc cctgctgccg cagacgcaat gcacgcgctg
       61 tggttatccg gcgtgcatgg attatgcgaa cgcgatcgcg ggcggcgaag cgaacatcaa
      121 ccagtgtccg ccaggcggaa ccgcgggcat catcgcactg gcgagtttgc tcgcgcgcgc
      181 gccgctgccg ctgaatccaa aaaatggcgt cgagaagcca cgcgaaatcg cgctgatcga
      241 cgaggaagtc tgtatcggct gcaccaaatg catccaggcc tgcccggtgg atgccatcgt
      301 cggcgcatcg aaactcatgc acacggtcat cgcggacgaa tgcaccggct gcgagctgtg
      361 catcgcgccc tgcccggtcg attgcatcgc gttgattccc gcatcgattc ccgcagatcc
      421 gggccactac cgcgcgcgct acatcgcaca cacgcggcgc gaggaactcc gaaatgcgga
      481 acgcgccgcg gaacttgccg cacgcaagtc cagggtcaac gccggcgacg ccgtcgccgc
      541 cgcgctggcg cgcgcacgcg cgaagaaggc cggcgcatga acgccgccaa gcgcgcggaa
      601 atcttccgcc gcctgcgtga ggcgaatccg aaaccgaaga cggaactcga atacgccacg
      661 ccgttccagt tgctgatcgc ggtgatgctt tcggcgcagg ccaccgacgt cggcgtgaac
      721 aaggcgacgc ggcggctgtt tcccgtcgcg ggaacggcgc tcgcgatgct cgcactcggc
      781 gaagaaaagc tgaaaacgtc tatttccacc atcggcctgt tcaacaccaa ggcgaagaac
      841 gtcatcgcga cctgcgcgat cctcgtcgaa caattcgacg gcgaggtgcc ggcttcgcgc
      901 gaggcgctcg aagccctgcc cggcgtcggc cgcaagaccg cgaacgtggt gctgaacaca
      961 gcgttcggcg aaccgaccat tgccgtcgac acgcatatct tccgcgtcgc caaccgcacc
     1021 gggctcgccc ccggcaagac cgtgcgcgca gtcgaggaca aactcgaaaa agtggtgccg
     1081 gatgaattca agcgcgacgc ccaccactgg ctgatcctgc acggccgcta cgtgtgcaag
     1141 gcgcgcaagc cggattgccc gaactgcatc atccgcgagc tgtgcgaata tcggcacaag
     1201 acgcgctgaa aaaaaacggg cgccgaggcg cccgttcaac cctgaccgag gagagcactc
     1261 gatcgtcaga acgcgatttc cgcccacacg ccgatttcgt tgctcttggt cgtattgacg
     1321 gcgtcggcaa gtttttcgtg cttcagaact gcggcgaggc gcacgccctt gcgcgcattc
     1381 cattccaggc cggcgttgta gtaggtgtcc ttgagttgcg gattgatgtc cttgctctgg
     1441 ttggcgtggt cgtagcgcgc gaacacggag taatccggcg cgaacttgaa ctggccccag
     1501 gccgagtagc cgtcggacga at
//
LOCUS       DASRVO010000107         1983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_184838, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000107 DASRVO010000000
VERSION     DASRVO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184838"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /gene="tilS"
                     /locus_tag="VFF05_01240"
     CDS             <1..614
                     /gene="tilS"
                     /locus_tag="VFF05_01240"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZW50460.1"
                     /translation="AHRLRLAQLRSRPAPAAGFLLREYLAARGLRLPSTRRLAEMLRQ
                     LTGATTGARVEFVHDGAVLRVYRGELRLERLDRVSAEFGPVRWTGQRRIALPALGGEM
                     CFERVRGAGVALAKLQGRLVQLRLRRGGERLQPDSRRPRRTLKNLFQEAGLPPWQRDR
                     LPLLFCGEDLVWAPGIGVDVSYLTPAGGAGLVPQWRSCVDATH"
     gene            689..1114
                     /gene="ndk"
                     /locus_tag="VFF05_01245"
     CDS             689..1114
                     /gene="ndk"
                     /locus_tag="VFF05_01245"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZW50461.1"
                     /translation="MAVERTLSIIKPDAVNKSVIGQICARFEAAGLKIVAARMMHLSR
                     AQAEGFYAVHRERPFFRDLVEFMISGPVQVQVLEGEGAIAKNRELMGATDPKKAAKGT
                     IRADFADSIDANAVHGSDGPDTARTEIGYFFPACEIFSR"
     gene            1134..>1983
                     /gene="rlmN"
                     /locus_tag="VFF05_01250"
     CDS             1134..>1983
                     /gene="rlmN"
                     /locus_tag="VFF05_01250"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZW50462.1"
                     /translation="MSDNLLNFDAHAMERHFARLGEKAFRARQVLRWIHQYGESDFAR
                     MSDLAKDLRAKLAQAARVEAPAVVADDVAPDGTRKWLLKVDNANSIESVFIPEDDRGT
                     LCVSSQAGCVLDCAFCSTGKQGFNRNLTTAEIIGQLWLANRLLGRDPKGQRVITNVVL
                     MGMGEPLLNLDAVIPALRLMLDDNAYGLSRRRVTVSTSGLVPGMDRLRDECPVALAVS
                     LHAPNDALRDRLVPLNRKYPLRDLIKACNRYLDQAPRDFITFEYVMLAGVNDSDGHAR
                     ELTAIAR"
BASE COUNT          314 a          630 c          677 g          362 t
ORIGIN      
        1 cggcgcaccg actgcggctc gcgcagttgc gctcccgccc cgctccggcg gcggggttct
       61 tgctgcgcga gtatctcgcc gcccgcggac tgcggctccc ttcgacccgg cggttggctg
      121 aaatgctgcg tcagctaaca ggcgcgacga ccggcgcgcg cgtggagttt gtgcacgacg
      181 gcgcagtctt acgcgtttac cgcggcgagc tgcgcctgga gcgcctggat cgcgtatcgg
      241 ccgagttcgg gccggtgcgc tggaccggcc agcgccgcat cgcgctgccc gcgctgggtg
      301 gcgaaatgtg cttcgagcgc gtgcgcggag ccggcgtcgc tctcgcgaag ctgcagggtc
      361 gactggtcca gcttcgcttg cggcgaggcg gcgaacggct ccaacctgat tcgcggcgac
      421 cccgtcgtac cctcaagaat ctgtttcagg aggctggcct gccgccgtgg cagcgcgatc
      481 gcctgccact gctgttctgc ggcgaggact tggtctgggc gcccgggatc ggcgtcgacg
      541 tcagctatct caccccggcc ggaggcgccg ggctggtgcc gcaatggcgt tcctgcgtcg
      601 acgcaactca ttgaatttac gcggttcgga accgtgccat ggtagaattt cggactaact
      661 ttttcaagct tgcgagggag aacgaggaat ggcggtcgag aggacgctgt ccatcatcaa
      721 gccggacgca gtgaacaaga gcgtgatcgg ccagatctgc gcacgtttcg aagcggcggg
      781 gctcaagatt gtcgcggcgc gcatgatgca tctgtcgcgc gcgcaggccg aaggcttcta
      841 tgcggtgcat cgcgagcggc ccttcttccg cgacctggtc gagttcatga tttcaggccc
      901 ggtgcaggtg caggtcctcg aaggcgaggg cgcgatcgcc aagaaccgtg aacttatggg
      961 cgccaccgat ccgaagaagg cggccaaggg tacgatccgc gcggatttcg cggattcgat
     1021 cgacgccaac gcagtgcacg gctcggacgg gccggacacc gcgcgcaccg agattgggta
     1081 tttcttcccc gcgtgcgaga ttttttcgcg ctgaagcggg gcacagagcg gcgatgagcg
     1141 acaacctgct gaatttcgac gcgcatgcga tggaacgcca tttcgcccgg ctgggcgaga
     1201 aggcgtttcg cgcgcggcag gtgctgcgct ggattcatca gtatggggag tcggacttcg
     1261 cgcgcatgag cgacctcgcc aaggacctgc gtgccaagct cgcgcaagcg gcgcgcgtgg
     1321 aggcgcctgc ggtcgtcgcc gacgacgtgg cgcccgatgg cacgcgcaag tggctgctca
     1381 aggtcgacaa cgcgaactcg atcgagtcgg tgttcatccc ggaagacgac cgcgggacct
     1441 tgtgcgtgtc cagtcaggcc ggttgcgtgc tcgattgtgc cttctgctcc accgggaaac
     1501 agggattcaa ccgcaatttg acgaccgcgg agattattgg tcagctttgg ctcgccaatc
     1561 ggctgctggg tcgcgacccg aagggccagc gcgtcatcac gaacgtggtc ctgatgggca
     1621 tgggcgagcc gctgctcaat ctcgacgcgg tgattccggc gctgcgcctg atgctcgacg
     1681 acaacgccta cggattgtcg cggcgccgcg tcacggtctc gacatccggc ttggttccgg
     1741 gcatggaccg cctgcgcgat gaatgtcccg tcgcgcttgc cgtctcgctg catgcgccga
     1801 acgatgcctt gcgcgaccgc cttgtgccgc tgaaccgcaa gtatccgctg cgcgatctga
     1861 tcaaggcctg caaccgctac ctcgatcagg cgccgcgcga cttcatcacc ttcgagtacg
     1921 tgatgctcgc gggcgtcaac gattcggacg gccacgcccg cgagttgacc gccattgccc
     1981 gga
//
LOCUS       DASRVO010000108         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_288785, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000108 DASRVO010000000
VERSION     DASRVO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288785"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..751)
                     /locus_tag="VFF05_01255"
     CDS             complement(83..751)
                     /locus_tag="VFF05_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50463.1"
                     /translation="MKRFALIALSAAFAVGSAFAAGNADLDIDKVNGSIHVPDNATVG
                     KLSTVNGSIHVGDGAHAVDATTVNGSIHIGANATLDALETVNGGIHTGAGAKIAKTIE
                     AVNGSITLGSGADVTGKVSNVNGGIALIAAHVGGGIETTSGDIDVGANSRVAGGILVN
                     QDNSWFHFGVSRDPRIVIGPHAEVKGDLVFKRTVQLYVSDSATVGKIDGAKAIPFTGD
                     KPPQ"
     gene            851..>1059
                     /locus_tag="VFF05_01260"
     CDS             851..>1059
                     /locus_tag="VFF05_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972327.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZW50464.1"
                     /translation="MSRPKPVLLLILDGWGHREARDNNAIAQANAPNWRRLLATCPHT
                     LVQTHGLYVGLPDGQMGNSEVGHMN"
BASE COUNT          181 a          321 c          350 g          207 t
ORIGIN      
        1 caatcgcgaa cgtaacctga acgccgcgat cgtccgagcg tttcacgggc gccgcgaggg
       61 aacgcggcaa gccatgcaaa ggctactgcg gcggtttgtc gccagtgaac gggatcgctt
      121 tcgcgccgtc gatttttccg accgtggcgc tgtcgctgac gtaaagctgc acggtacgct
      181 tgaaaacgag atcgcctttc acctcggcat gcgggccgat gacgatgcgc gggtcgcggc
      241 tgacgccgaa gtggaaccag ctgttgtcct gattgacgag aattccgccg gcgacgcgcg
      301 aattcgcgcc gacgtcgatg tcgcccgaag tcgtttcgat gccgccgccg acgtgcgccg
      361 cgatcagcgc gatgccgccg ttgacgttgg aaaccttgcc ggtcacgtcc gcacccgagc
      421 ccagcgtgat cgaaccgttg accgcctcga tggtcttggc gatctttgcg ccggcaccgg
      481 tatggatgcc gccattgacg gtctcgagcg cgtcgagtgt cgcattggcg ccgatatgga
      541 tgctgccgtt gacggtggtc gcgtccacgg cgtgcgcacc gtcaccgaca tggatgctgc
      601 cgttgaccgt cgacaacttg ccgaccgtgg cgttgtccgg cacgtggatg ctgccattga
      661 ccttgtcgat atccaggtcc gcattgccgg cggcgaaagc gctgccgacg gcgaaggcgg
      721 cggacaatgc aatgagtgcg aatcgtttca tggggctgct ccggtgcgag tcgtgttgcg
      781 cgttggatgc ggcgggtcgc tgcggggttt aatcggctaa cattatccac ccgatttttc
      841 agagtgcgcc atgtccagac caaagcccgt gttgttgttg atcctggatg gatggggcca
      901 tcgcgaggcg cgcgacaaca acgccattgc acaggcgaac gcgccgaact ggcggcgcct
      961 gctcgccacc tgtccgcata cgctggtgca gacgcacggg ctgtatgtcg gcctgccgga
     1021 cggacagatg ggcaattccg aggtcggcca catgaacat
//
LOCUS       DASRVO010000109         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_132796, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000109 DASRVO010000000
VERSION     DASRVO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132796"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFF05_01265"
     CDS             complement(<1..582)
                     /locus_tag="VFF05_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZW50465.1"
                     /translation="MNPLLRLRAWLRHSLREPRLDLPLLAALILLALCGLTTLYSASD
                     LDRHLVFGQALRFALGGLFLLLLSRVPPNVLRNWTPWLYFASLALLVAVAAIGQGRGA
                     NRWLSLGVVRFQPSELVKLTLPMMIAWYLHARELPPRWLTLGAVALLIVSPALLIAKQ
                     PDLGTALLVTASGVFAIFLAGLAWWRIFVLLGAA"
     gene            complement(579..>1953)
                     /gene="mrdA"
                     /locus_tag="VFF05_01270"
     CDS             complement(579..>1953)
                     /gene="mrdA"
                     /locus_tag="VFF05_01270"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZW50466.1"
                     /translation="TRAYPRGADFAHVVGYVGRIDAADAADLDADEYAGTTQIGKTGI
                     ERSYERELHGAPGYEQVEVNVDKRVIGKPISRVAPTPGKNLYLSIDARLQRVAFDALG
                     GRPGAVAAIDPRNGEVLALASAPSFDPNLFVNGIGRADYAALMNNPDKPLLDRAIAGG
                     FTPGSTVKPYLGLGGLELGLRRPQDTVLSTGEFFIPGQSRGYRDDVRGGQGLVNLRQA
                     IARSVNTYFYSLALDMGIDRFSAWMANFGFGKPTGIDLPGEASGVLPSRAWKRAKMEQ
                     PFYPGESVIAGIGQGFWVITPIQLASALATLADGGVRHVPHLLRALQAGMNAPRVPVT
                     QPKSAPSFVRNPANWDAVRQGMIDVVNDPSGTAHGLGEGFPYVIAGKTGTAERYSRTS
                     EEWTSIMASPISRHEVLFEAFAPADAPRVAVVVALKEGRSGAYDAAPIARRILDEWLK
                     DDGGHTP"
BASE COUNT          320 a          637 c          695 g          301 t
ORIGIN      
        1 cgccgcaccg agcagaacga agatgcgcca ccaggcgagt ccggcgagga agatcgcgaa
       61 cacgccgctg gcggtgacca gcaaggcggt gcccagatcc ggctgcttgg cgatgagcag
      121 tgccggcgac acgatcagca gtgcgaccgc gccaagcgtc agccagcgtg gcggcagctc
      181 acgtgcgtgc aggtaccagg cgatcatcat cggcagggtc agcttgacca gttccgaggg
      241 ctggaagcgc accacgccca gactcagcca gcggttcgcg ccgcggcctt gtccgattgc
      301 cgccacggcc acgagcagcg cgaggctggc gaagtaaagc cacggcgtcc agttgcgcag
      361 cacgttcggc ggcacgcgcg agagcagaag caggaacaat ccgccaagcg cgaagcgcag
      421 cgcctgaccg aatacaagat ggcggtccag atcgctggcg ctgtacaacg tggttaggcc
      481 gcacagggcg agcaggatca gcgcggcgag cagcggcagg tccaggcgcg gttcgcgcag
      541 actgtggcgc agccaggcgc gcaggcgcag cagcggattc atggcgtgtg gcctccgtcg
      601 tccttgagcc attcgtcgag gatccggcgc gcgatcggcg ccgcgtcgta ggcgccggag
      661 cggccttcct tgagcgcaac caccaccgcg acgcgcggcg cgtcggctgg cgcgaacgcc
      721 tcgaacagca cttcgtggcg gctgatcggc gaggccatga tgctggtcca ttcctcgctg
      781 gtgcgcgaat agcgctcggc cgtgccggtc ttgccggcga tgacgtaggg gaatccttcg
      841 ccgagtccat gcgccgtgcc ggatggatcg ttgacgacgt cgatcatgcc ctggcgcacc
      901 gcgtcccagt tggccggatt gcgcacgaaa ctcggtgcgg atttcggttg cgtcaccggc
      961 acgcgcggcg cgttcatgcc cgcctgcagc gcacgcaaca ggtgcgggac gtggcgcacg
     1021 ccgccatcgg cgagcgtggc cagagcggac gccaactgga tcggcgtgat cacccagaaa
     1081 ccctggccga tgccggcgat cacgctttcg cccggataga acggctgttc catctttgcc
     1141 cgcttccagg cgcgcgacgg cagcacgccg gacgcttcgc cgggcaggtc gatgccggtg
     1201 ggcttgccga aaccgaaatt cgccatccac gcgctgaagc ggtcgatgcc catgtccagc
     1261 gcgagcgaat agaagtaggt gttgaccgag cgcgcgatgg cctggcgcag gttcaccagt
     1321 ccctggccgc cgcgcacgtc gtcgcggtaa ccgcgcgact ggccgggaat gaagaattcg
     1381 ccggtcgaaa gcacggtgtc ttgcggtcgg cgcaggccga gttcgaggcc gccaaggccc
     1441 agatacggtt tgaccgtgga acccggcgtg aagccgccgg cgatggcgcg gtcgagcagg
     1501 ggtttgtccg ggttgttcat caacgcggcg tagtcggcgc ggccgatgcc gttgacgaac
     1561 aggttcggat cgaagctggg tgcgcttgcc agtgcgagca cttcgccgtt gcgcggatcg
     1621 atcgcggcca ccgcgccggg gcgtccgccc agcgcatcga aggccacgcg ctgcaggcgc
     1681 gcgtcgatgc tcaggtacag gttcttgccc ggcgtcggcg cgacgcggct gatcggtttg
     1741 ccgatcacgc gcttgtccac gttcacttcg acctgttcgt agccgggcgc gccgtgcaat
     1801 tcgcgttcgt agctgcgttc gatgccggtc ttgccgatct gcgtggtgcc ggcgtattcg
     1861 tcggcatcga ggtcggccgc gtccgctgcg tcgatgcggc cgacatagcc gacgacgtgc
     1921 gcgaaatccg cgccacgcgg ataggcgcgg gtc
//
LOCUS       DASRVO010000110         1048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_2893, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000110 DASRVO010000000
VERSION     DASRVO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFF05_01275"
     CDS             <1..586
                     /locus_tag="VFF05_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HZW50467.1"
                     /translation="TKWGAFAAAWVLLALAACASPSPRTEFYTLDTEAPAGRGATSAA
                     PDQPAVKVSVWQVSVPEMVDRSELVLRTAPNRVQISDFHRWAEPLRFGIARVLADDLA
                     LRLGSGFMVVVGQPAGIQPDVRVSFDVQKFEAVPGEGVAVEALWNIRPARGEARSGRS
                     MVSEREPSRDYAALVAAYSRALAQVAREVSVAVR"
     gene            complement(809..>1048)
                     /locus_tag="VFF05_01280"
     CDS             complement(809..>1048)
                     /locus_tag="VFF05_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50468.1"
                     /translation="LYVLLGDRAVEIVADRGIRARVGQDEWEAICHEMQAEFAKGAFE
                     RGSLAGIRRVGDLLAAHFPARTAQRNELPDKPALL"
BASE COUNT          159 a          337 c          360 g          192 t
ORIGIN      
        1 gacgaaatgg ggcgcctttg ccgccgcatg ggtgctgctc gctcttgcag cctgtgcgtc
       61 gccgtctccg cggaccgagt tttacacgct ggacaccgag gcgcccgccg gacggggagc
      121 gacgagcgct gcgcccgatc agccggccgt caaggtgtcg gtgtggcagg taagcgttcc
      181 ggaaatggtg gaccgctccg agctcgtgct gcgcactgca cccaaccggg tccagataag
      241 cgactttcac cgctgggcgg agcctttgcg attcggcatc gcccgcgtgc tggccgatga
      301 tcttgcgctt cgtctgggca gcggcttcat ggtggtcgtc ggccaaccag ccggcattca
      361 gccggacgtg cgcgtttcgt tcgacgtgca gaaattcgaa gcggtgccgg gcgagggcgt
      421 ggccgtcgag gcgctatgga atatccggcc agcgcggggc gaggcgcgca gcggccgctc
      481 gatggtctcg gagcgcgaac cctccaggga ctatgcggcc ctggtggccg cgtacagccg
      541 cgcgctggca caggtggcgc gcgaggtgag cgtcgcggtg cgataggact gcatcacacc
      601 atggcgcgct agtgtcccga gtctctaatg agccgaacgt ttcttggtcg cctgcgcaat
      661 cggtttgctt cgtagggcgg attagccgaa ggcgtaatcc gccgcgtcag tggctgcctt
      721 tttccggtgc atcggcgcat tacgccggtg catcggcgca ttacgccgct ttcgcggcta
      781 atgcgcccta cgaaaactgc aagcgtgact agagcagcgc cggcttgtcg ggcagctcgt
      841 tgcgctgagc cgtgcgcgcg gggaaatgcg ccgcaagcag atcgcccact ctgcgaattc
      901 ccgccagaga gccgcgctcg aacgctcctt tggcgaactc cgcctgcatt tcgtggcaga
      961 tcgcttccca ttcgtcctgt ccaacccgcg cccggatgcc gcggtcggcg acgatctcga
     1021 cggcgcgatc gccgagcagc acgtagag
//
LOCUS       DASRVO010000111         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_262868, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000111 DASRVO010000000
VERSION     DASRVO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262868"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..495
                     /locus_tag="VFF05_01285"
                     /pseudo
     CDS             31..495
                     /locus_tag="VFF05_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            complement(551..>1503)
                     /locus_tag="VFF05_01290"
     CDS             complement(551..>1503)
                     /locus_tag="VFF05_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW50469.1"
                     /translation="MLKLLGASEAAEKFASSREEAAPLVQIDAAPAVAQALGGHALLV
                     EDNPVNRQVAQRLLTLLGLSFAVAENGKEALDALEHDAFDVVLLDCQMPVMDGYTAVR
                     ILRQNEATKALKHMPVIAMTANAMAGDREKCLRAGMDDYLSKPLNRALLEQTLRRWIP
                     AGATSRAPQASPTGAVAQPRPTPAPAFVAQAPPIPAVSAASAALDADVVRDLLEVMGD
                     EFTDLVRVYLEDTPKSIALLEQAAGTADNQGLIAPAHSLKSTSANLGALSLAELAKRL
                     EHGARSGDLGSEAPVLVAELSRTFARVHAELQSLLAKSAA"
BASE COUNT          234 a          464 c          521 g          284 t
ORIGIN      
        1 gaatccgggc atgatttgcg gagtttgcgt gtgatcgatc cggatggcta tcgcccgaat
       61 gtcggcatca tcctggtgcg cgacgacggt tcggtgttct gggcgcggcg tgcctaccgg
      121 gacggctggc agttcccgca aggcggcatg aacagcgacg agacgccgct ggaagccatg
      181 taccgcgagc tggaagaaga aaccggattg cgccctgagc atgtgcaagt gctgggtgcg
      241 acgcccggct ggttgcgcta tcgcctgccg tacaaatacc tgcgccacaa ggaaaagccc
      301 atgtgcatcg ggcagaagca ggtgtggttc ctgctgcgct tcatcggcca ggaaacgcat
      361 gtgcgtctgg atgccacgga ttcgccggag ttcgacctgt ggcgctgggt ggatttttgg
      421 tatccgctgg cccacgtcgt gccgttcaag cgcgatgtct acgagcgtgc cctgcggcat
      481 ctggcaccgc ttgccgaagc gttgcgcgcg agcgtgaatc tggacatcgt ttccggctga
      541 ttgcccgggt tcaagccgcg ctcttcgcga gcagcgattg caactccgcg tgcacgcgcg
      601 cgaacgtgcg cgacagttcc gccaccagca ccggcgcttc gctgcccaga tcgccgctgc
      661 gcgcgccgtg ttccaggcgc ttggccagtt ccgccaggct cagcgcgccg aggttggcgc
      721 tggtggactt gagcgaatgc gccggcgcga tcaggccttg gttgtcggca gtgccggcag
      781 cttgttccaa taatgcgatc gacttggggg tgtcttccag atacacgcgc accaggtcgg
      841 tgaattcgtc gcccatcact tcgagcaggt cgcgcacgac atcggcgtcg agcgcggcgc
      901 tcgcagcgga aaccgccggg atcggcggcg cctgcgcgac gaaggcgggc gcgggcgtcg
      961 gccgcggctg cgccacggcg cccgttggcg atgcttgcgg cgcgcgcgag gtggcgccgg
     1021 ccggaatcca gcgccgcagg gtttgttcga gcagggcgcg gttgagcggt ttggacaggt
     1081 aatcgtccat gcccgcgcgc aggcattttt cgcggtcgcc ggccatcgcg ttcgcggtca
     1141 tcgcgatcac cggcatgtgt ttcaacgctt tcgtggcttc gttctggcgc aagatacgca
     1201 cggccgtata gccgtccatc acaggcatct ggcaatcgag caggacaacg tcgaaggcgt
     1261 cgtgttcgag cgcatccagc gcttccttgc cgttttcggc gacggcgaaa ctcaaaccga
     1321 gcagggtgag caggcgctgt gcgacctggc ggttgaccgg attgtcctcg accagcaatg
     1381 cgtgtccgcc gagcgcttgc gcaacggcgg gtgcggcatc gatctgcacc agcggtgcgg
     1441 cctcttctcg cgaagaggcg aatttctccg cggcctcgct ggcgccgagc agcttgagca
     1501 tgg
//
LOCUS       DASRVO010000112         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_366498, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000112 DASRVO010000000
VERSION     DASRVO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366498"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="gltX"
                     /locus_tag="VFF05_01295"
     CDS             <1..1140
                     /gene="gltX"
                     /locus_tag="VFF05_01295"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZW50470.1"
                     /translation="LVRAGKAYYAYETKEELDAMRARQMEQNIKPRYAGVYREQNAPL
                     RDDPNRVIRFRNPASGSVVFNDKVKGRIEWSNDELDDLVMLRSDGFPTYNFAVVVDDI
                     DMRVSEVIRGDDHVNNTPRQINIYNALEADIPEFAHLPMILGPDGQKLSKRHGAVGVM
                     QYRDDGFLPHAVLNYLVRLGWSHGDQEIFSIKEMIDLFDVADVNKAASRFDVEKLSWL
                     NQHYLKTGDPEALAPELEWQLRQQGYDPAHGPRAADVVVALRDRARTLKEMADKASIW
                     YGPITQWDDAAVAKHLKTPKAAAALTAAHEQLAIVPEWNVESVHKAIEAAAEAIGEGM
                     GKVAQPLRVAMTGTQVSPSIDHTVFLAGRERALKRIEDAITRIGA"
     gene            1144..>1377
                     /locus_tag="VFF05_01300"
     CDS             1144..>1377
                     /locus_tag="VFF05_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50471.1"
                     /translation="MACDFSALTNLDPTDPDAAQRLLDQCTQTLTDPTLWIWAIVFTV
                     VCAAVGALIGKYKNAVMRDAVLGAALGPIGWVIS"
BASE COUNT          269 a          442 c          444 g          222 t
ORIGIN      
        1 ctggtgcgtg cgggcaaggc gtattacgcg tacgagacga aggaagaact cgatgcgatg
       61 cgcgcgcggc agatggagca gaacatcaag ccgcgttacg ccggcgtcta ccgcgagcag
      121 aatgcgccgc tgcgcgatga cccgaatcgg gtgatccgtt tcagaaatcc ggcttctggt
      181 tccgtcgtgt tcaacgacaa ggtcaagggc cgcatcgagt ggagcaacga cgaactcgac
      241 gacctcgtca tgctgcgttc ggacggattc ccgacctaca acttcgccgt cgtcgtcgac
      301 gacatcgaca tgcgcgtgag cgaggtgatt cgtggcgacg accacgtcaa caacacgccg
      361 cgccagatca acatctacaa cgcgctggaa gccgacatcc ccgagttcgc gcacctgccg
      421 atgatcctcg ggccggacgg gcagaagctg tccaagcgcc acggtgcggt cggcgtgatg
      481 cagtatcgcg acgacggctt cctgccgcat gcggtgctca attacctggt gcgcctgggc
      541 tggtcgcacg gcgaccagga aatcttctcg atcaaggaaa tgatcgactt gttcgacgtt
      601 gccgacgtca acaaggcggc gtcgcgcttc gacgtggaga agctttcctg gctcaaccag
      661 cattacctca agaccggtga tcccgaagcg ctggcgccgg agctggaatg gcagttgcgc
      721 cagcaaggct acgatcctgc gcatggccca cgtgcggccg acgtggtcgt ggccctgcgc
      781 gaccgcgccc gaacgctcaa ggaaatggcc gacaaggcca gcatctggta cggcccgatc
      841 acgcagtggg acgacgcggc cgtggccaag cacctgaaga cgccgaaagc cgccgccgcg
      901 ctgaccgccg cgcacgaaca actcgccatc gtgccggaat ggaacgtgga aagcgtgcac
      961 aaggccatcg aggccgccgc cgaggcgatc ggcgaaggca tgggcaaggt cgcgcagccg
     1021 ctgcgcgtgg cgatgaccgg cacgcaggtg tcgccgtcca tcgatcacac cgtgttcctg
     1081 gccggacgcg aacgcgcact caagcgcatc gaggacgcga ttaccaggat cggcgcctga
     1141 cccgtggcct gtgatttttc cgcgctgacg aacctggatc cgaccgatcc ggacgcggcg
     1201 caacgcctgc tcgatcaatg cacgcagacc ctgaccgatc cgacgttgtg gatctgggcc
     1261 attgtcttca ccgttgtctg cgccgcggtc ggcgcgctga tcggcaagta caagaacgcg
     1321 gtcatgcgcg atgccgtgct cggtgccgcg cttggcccga tcggctgggt catctcg
//
LOCUS       DASRVO010000113         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_236323, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000113 DASRVO010000000
VERSION     DASRVO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236323"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VFF05_01305"
     CDS             complement(<1..288)
                     /locus_tag="VFF05_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007972203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZW50472.1"
                     /translation="MLWLKRAGMAFAVFVAIAFVLDRLFPLPLPDANGGSTVVLARDG
                     TPLRAFPDVDGAWRYPVTPDEVSPLYLQALLTYEDRWFWWHPGVNPVALGRA"
     gene            complement(354..>1128)
                     /locus_tag="VFF05_01310"
     CDS             complement(354..>1128)
                     /locus_tag="VFF05_01310"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HZW50473.1"
                     /translation="LTSQQREADSEQKRYGFHWNWLSTQEQIAIARLGRELIQNGDTV
                     VAGTLAIGAKSTELSPDRIWSRAFDATDLRAGVRLNPSGDVPLYVSTDIAGVPTAPPT
                     PDDHYVSIRRAFYTLDGKAWTPAPLKEGDALIVGLTLEARQSMPDALVTDLLPGGLEI
                     ENFNLTDAKQWAGVVIDGITLSDRAQAAEVRHEEFRDDRYVAALKLDQGQAAHMFYLV
                     RAVSPGTYLVPPPLIEDMYLAQARGIGKSEPASIKVVEP"
BASE COUNT          202 a          384 c          361 g          181 t
ORIGIN      
        1 cgcgcgtccc agcgcaaccg gattcacgcc cggatgccac cagaaccagc gatcctcgta
       61 ggtcagcaac gcctgcaggt acagcggcga gacctcatcc ggcgtcaccg gataacgcca
      121 agcgccatcg acatccggaa acgcgcgcaa cggcgtgccg tcgcgtgcga gcaccaccgt
      181 gctgccgccg ttcgcgtccg gcagcggcaa ggggaacagg cgatcgagca cgaacgcgat
      241 cgccacaaaa accgcgaatg ccatccccgc gcgcttgagc caaagcagcg cgcgaacgcg
      301 cagacctccc gctccctcgg cgccagcgcg cgaaaggaac gcgaagcgcg gtttcacggc
      361 tcgaccacct tgatcgacgc cggttcgctc ttgccaatgc cgcgcgcctg cgccaggtac
      421 atgtcctcga tcagcggcgg cggcacgaga taagttccgg gcgacaccgc gcgcaccaga
      481 tagaacatgt gcgcggcctg accctggtcg agcttgagcg cggcgacgta gcggtcgtcg
      541 cggaattcct cgtgacgcac ctcggcggcc tgcgcgcgat cggacagcgt gatgccgtcg
      601 atcaccacgc cggcccattg cttggcgtcg gtcaggttga aattctcgat ttccagtccg
      661 ccgggcaaca gatcggtcac cagcgcatcg ggcatggact ggcgcgcttc aagggtcagg
      721 cccacgatca gcgcatcgcc ttccttgagc ggcgccggcg tccacgcctt gccgtcgagc
      781 gtgtagaacg cgcgacgaat ggagacgtag tggtcgtccg gtgtcggcgg cgctgtcggc
      841 acgccggcga tgtcggtgct cacgtacagc ggcacgtcgc cgctcggatt caggcgcaca
      901 cctgcgcgaa gatcggtcgc atcgaaagcg cggctccaga tgcgatcggg agacaattcc
      961 gtcgacttcg cgccgattgc gagcgtgcct gcaactaccg tatcgccgtt ctggatgagc
     1021 tcgcggccca gccgtgcgat ggcgatttgt tcctgcgtgc tgagccaatt ccagtggaac
     1081 ccatagcgct tttgttcgga atcggcttcg cgttgctgcg aggtcagc
//
LOCUS       DASRVO010000114         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_29153, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000114 DASRVO010000000
VERSION     DASRVO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29153"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1529
                     /locus_tag="VFF05_01315"
     CDS             <1..>1529
                     /locus_tag="VFF05_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW50474.1"
                     /translation="VDTAEADIHHDWDALIKTNLSPDQRELIDEVAKARVIADQAATK
                     LRGILKAQDMPALGKFADTELYPAIDPVTNRLQFLADVKMLDAERSVSGHLTRARAVG
                     WWRILVSLAALLFVFVVGRSILRNVYKGVKQLAQFARHARDPDYPAPEWNAAEGGELG
                     QIHAALTDMRADLLGYERDIKESEAYAQAASRAKSSFLASMSHEIRTPMVGVAGMLEL
                     LAHTNLDGEQRQQVEIAQNSAQSLLQIIGDILDFSKIEAGKLELNPAPLDLRALVRNS
                     TDNFLGTASAKGLKLECEIDERLAPAHLGDALRLRQILSNFLSNALKFTERGRVLVSL
                     ERLATEGSRELIAIRVKDSGIGVSKEDQARLFQPFAQAGAGGAQRVDSTGLGLSICRR
                     LAELMSGDIVMDSRVGAGTTMSLVVRLALADASLLQRDDGALLSVVRAAPSVDAARRE
                     GSLILLADDHPTNRAVIARQLNLLGYACESAHDGETALQMWRSGEYALLLTDLHMPRR
                     DG"
BASE COUNT          288 a          515 c          489 g          237 t
ORIGIN      
        1 gtcgacaccg ccgaagccga catccaccac gactgggacg ctctgatcaa gaccaatctt
       61 tcgccggatc agcgcgaact gatcgacgaa gtggccaagg cgcgcgtgat cgccgatcag
      121 gcggcaacga aattgcgcgg catcctcaag gcccaggaca tgcccgcact gggcaagttc
      181 gccgataccg aactgtatcc ggcgatcgac ccggtgacga accgcctgca attcctcgcc
      241 gacgtgaaga tgctcgacgc cgaacgcagc gtgagcgggc acctgacgcg tgcgcgtgcg
      301 gtcggctggt ggcgcatcct ggtgagtctt gcggcgctgc ttttcgtgtt cgtggtggga
      361 cgcagcattt tgcgcaatgt atacaaaggc gtgaagcaac tcgcgcaatt cgcccggcac
      421 gcgcgcgacc cggattaccc tgcgccggaa tggaacgccg ccgagggcgg cgaactcggc
      481 cagatccatg ccgcgctcac cgacatgcgt gcggacctgc tcggctacga acgggatatc
      541 aaggaaagcg aggcgtacgc gcaggcggcg agccgggcca agtcatcgtt cctggcctcg
      601 atgagccacg aaatccgcac gccgatggtc ggcgtggccg gcatgctgga actgctcgcg
      661 cacacgaacc tcgacggcga acagcgccag caggtcgaaa tcgcgcagaa ctcggcgcaa
      721 agccttctcc aaatcatcgg cgacatcctg gatttctcca agatcgaggc cggaaaactc
      781 gaactgaatc ccgcgccgct ggacctgcgc gcgctggtgc gcaattccac cgacaacttc
      841 ctcggaaccg catccgccaa gggtttgaaa ctcgagtgcg agatcgacga gcgcctcgcg
      901 cccgcgcacc tgggcgatgc gctgcgcctg cgccagattc tgtccaactt cctgtccaat
      961 gcgctcaagt tcaccgagcg cggccgggtg ctggtgtcgc tggaacggct ggcgacggag
     1021 ggttcgcgcg aactcatcgc catccgcgtg aaggattccg gcatcggtgt atccaaggaa
     1081 gatcaggcgc gtctgttcca gccgttcgca caggccggag ccggcggcgc acaacgcgtg
     1141 gacagtaccg gattggggct gtccatctgc cgtcgactgg ccgaactcat gtccggcgac
     1201 atcgtcatgg acagtcgcgt cggcgccggc accacgatga gcctggtcgt gcgcctcgcg
     1261 ctggcggatg cgagcctgct gcagcgcgac gacggtgccc tgctcagcgt cgtccgcgcc
     1321 gcgccgagcg tcgacgcagc caggcgcgaa ggcagcctga tcctgcttgc cgacgaccac
     1381 ccgaccaacc gcgccgtgat cgcgcgccag ctcaacctgc tcggctacgc ctgcgaaagc
     1441 gcgcacgatg gcgaaaccgc gctgcagatg tggcgcagcg gcgaatacgc gcttttgctc
     1501 accgacctgc acatgccgcg gcgcgacgg
//
LOCUS       DASRVO010000115         1622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_29237, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000115 DASRVO010000000
VERSION     DASRVO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29237"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..330)
                     /locus_tag="VFF05_01320"
     CDS             complement(1..330)
                     /locus_tag="VFF05_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HZW50475.1"
                     /translation="MQIPNTPFGTTDWSQIPPTERRGERGCAYWRTLNFGSIRVRMVE
                     YTPGYLADHWCTKGHILLCTAGELRTELEDGRTFLLTAGMSYQVGDDAEPHRSSTDCG
                     ATLFIVD"
     gene            complement(370..1158)
                     /locus_tag="VFF05_01325"
     CDS             complement(370..1158)
                     /locus_tag="VFF05_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZW50476.1"
                     /translation="MTMQRTFRYADSDSQVGDLYLPPTGIRPPVVCLLHGGFWRLPYG
                     REEMAPVAADLAARGYAVWNIEYRRIGEPGGGWPGAAEDVAAAVDHLAVLADRGTRLD
                     LGRVAVVGHSAGGHLALCAGARGARQRRFAPSKVVPSVVCGLAAVADLHEAFLLDAGN
                     GAVQSLLGGSPQRHPERYEQASPRCMLPLGVRQLILHGSADDALPPRLSRTYVQAARG
                     AGDGVDHIELPGMGHMEYLDPGSRAHAHLCNWLGVMLAHRTPGT"
     gene            complement(1295..>1622)
                     /locus_tag="VFF05_01330"
     CDS             complement(1295..>1622)
                     /locus_tag="VFF05_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HZW50477.1"
                     /translation="FLFVSGMTGLDPETGKLAGETIQEQTTRAMRNCESILSVAGATL
                     HDVVEVQVLLARPEDFAGLNESYAAFFPTDPPARSVAKLGVEIPGVLVSIRLVAVAPY
                     SMQRTH"
BASE COUNT          288 a          519 c          545 g          270 t
ORIGIN      
        1 tcaatcgacg atgaatagcg ttgcgccgca gtcggtggac gaccggtgag gctccgcgtc
       61 gtcgccgacc tgataactca tgcccgcagt gagcaggaaa gtccgcccgt cttcgagttc
      121 ggtccgaagc tcgcccgccg tgcacaggag aatatggccc ttggtgcacc aatggtcggc
      181 caggtaaccg ggtgtgtact cgaccatgcg cacgcgaatg ctgccgaagt ttagcgtgcg
      241 ccaatacgcg catccgcgtt cgccgcgccg ttcggtcggc gggatttggg accaatcggt
      301 cgtgccgaac ggcgtgtttg gaatctgcat ggcggctccc gtcgttgtgg ttctcgcctg
      361 gcgaagacgt caggtgccgg gcgttcggtg ggccagcatg acaccgagcc agttgcacag
      421 atgcgcatgc gcccggctgc cgggatccag atattccatg tgacccatcc cgggcagctc
      481 gatgtggtcc acgccgtcgc cggcgccgcg cgccgcctgc acataggtac gcgagagacg
      541 cggcggaaga gcgtcatccg cggatccgtg aagtatcagt tggcgcacgc cgagcgggag
      601 catgcaccgc ggagacgcct gttcatatcg ttcggggtgc cgctgcggcg atccgccgag
      661 cagtgattgc acggcgccgt tgccggcatc gagcaggaag gcttcgtgca agtcggcgac
      721 ggcggccagt ccgcacacca ccgagggaac aactttcgat ggcgcgaaac ggcgctgcct
      781 cgcaccacgc gcgccagcgc acagcgcaag gtggccgccg gccgaatgcc cgacgacggc
      841 gacacggccg agatccaggc gtgtgccgcg gtccgcgagg acggccaggt gatcgacggc
      901 cgctgccacg tcttccgctg ctccgggcca gccgccaccc ggctcgccga tgcggcggta
      961 ctcgatgttc caaaccgcat aaccgcgcgc cgccaggtct gcggcaacgg gcgccatttc
     1021 ctcgcgtcca tacggcagcc gccagaaccc gccgtgcagg aggcaaacga ccggcggtcg
     1081 tatgccggtc ggcgggagat acagatcgcc gacttggctg tcggaatcag cgtagcggaa
     1141 agttcgttgc attgtcatcg aggatttacg atgtccgttc cggatgcggt gacggcccag
     1201 cgacgcgcaa gatcaggaag cacgagggaa cgatcatgtg tgggcttgcc ggtcgtaatc
     1261 gcgccgcgcc agatgcatcg cgtcatgcaa cgaatcaatg cgtccgctgc atcgaatagg
     1321 gtgcgaccgc caccaggcga atggagacca gcacgccggg tatttcgacg cccagtttcg
     1381 cgaccgagcg ggccggcgga tcggtgggga aaaaggcggc ataggactca ttcagtcctg
     1441 cgaaatcctc gggccgcgcc agcaggacct gaacctccac gacatcgtgc agcgtggcgc
     1501 cggcgacgct caggatgctt tcgcagttcc tcatggcgcg cgtcgtctgt tcctggatgg
     1561 tttcgccggc gagttttccc gtttcggggt ccagccccgt catgccactg acgaagagga
     1621 ac
//
LOCUS       DASRVO010000116         1443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_314794, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000116 DASRVO010000000
VERSION     DASRVO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314794"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..>1443
                     /locus_tag="VFF05_01335"
     CDS             185..>1443
                     /locus_tag="VFF05_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZW50478.1"
                     /translation="MARFVLISLIFTSIQLAALALGWAALRMVNATRAYATGESYYSK
                     GANAAVLSLYKYADTGNAADWEAFISSLGVTFGDRAAREALERNRPGVAAATAGLLRG
                     RTNINDIPDVILVFRLFQRWGPFKAAVEDWQQGDATIDRLLTVASRLHSLWHDGAVTD
                     ATRAQALRDVDRLNGRLISLENSFSGNIASTARTATGLVTHTLLVASLLLWSFGVGLA
                     WRTYRKGLAAEVGLWNSEERFRNFAETASDWFWETDPALNITYLSERFYEATGVRPQD
                     LLGRPAATAGLRDPVDSGAPLFAQMLAEHRAFRDHTCRYVRAPGDEQYWKLGGSPVLA
                     GDGTFLGFRGTGSNVTAAARAEQALREAKELSDVANRAKSEFLANMSHELRTPLNAII
                     GFSEIMDRGTFGSLGSDRYKSYVRDIIR"
BASE COUNT          252 a          466 c          472 g          253 t
ORIGIN      
        1 cgatgctgct ttcagcggtt ggggaataac cgcggcgatc gctgacgatg aacgggtgca
       61 gcgaacacac agtcggggcg accggcggcc gcgcgacgcc ggtctggcta ccgggcgcgc
      121 gtcctgccga gcgtagcggg ctgcgatgag gcagtcggcg acccgcggcg taacacgcgc
      181 gcacgtggcg cgcttcgtgt tgatcagcct gatctttacc agcatccagc tcgcggcgct
      241 ggcgctcggc tgggcggcgc tgcgaatggt caatgcgacg cgcgcctacg caaccggcga
      301 atcgtactac tcgaagggcg ccaatgctgc cgtactgagt ttgtacaaat acgccgacac
      361 cggtaacgcg gccgattggg aagcgtttat ttcctcgctc ggcgtgacgt tcggtgaccg
      421 ggcggcgcgc gaggcgttgg aaaggaaccg gccgggcgtc gccgccgcga ccgccgggct
      481 gctgcgcggg cggacgaaca tcaacgatat tcccgacgtc atcctggtgt tccggctctt
      541 ccaacgctgg ggaccgttca aggcggcggt cgaggattgg cagcagggcg acgcaacgat
      601 cgatcgtttg ctcaccgtgg cttcccggct gcactcgctc tggcacgacg gagcggttac
      661 cgacgcgacg cgggcacagg cgctccgtga tgtagaccgc ctcaatggta gactgatcag
      721 tcttgaaaac agtttttccg gcaatatcgc cagcacggcg cgaaccgcta ccggcctggt
      781 gacgcacacg ttgctggtag ccagtctgct gctctggtcg ttcggcgtcg gattagcctg
      841 gcggacctat cgcaaaggcc ttgcggccga ggtcgggctg tggaacagcg aagaacgctt
      901 ccgcaatttc gccgagaccg cctcggactg gttctgggag accgatcctg ccctcaacat
      961 cacctatctc tccgaacgct tttacgaggc gaccggcgtc cggccgcagg acctgctcgg
     1021 gcgtcctgcg gcaacagccg ggcttcgcga cccagtcgac agcggggcgc cgctcttcgc
     1081 ccagatgctc gccgaacatc gcgccttccg cgatcacacc tgccgctatg tgagggcgcc
     1141 cggcgacgag caatattgga agcttggcgg ctcgccggta ttggccggtg acggtacgtt
     1201 tctcgggttt cgcggcaccg gcagcaatgt gaccgcggcg gcccgcgccg aacaggcgtt
     1261 gcgcgaagcg aaagagcttt ccgacgtggc aaatcgcgcc aaatccgaat tcctcgccaa
     1321 catgagccat gaactgcgca cgccgctcaa cgccatcatc ggcttttcgg aaatcatgga
     1381 tcgcggcacg ttcggctcgc tcggcagcga ccgctacaaa agctacgtgc gcgacatcat
     1441 ccg
//
LOCUS       DASRVO010000117         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_3097, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000117 DASRVO010000000
VERSION     DASRVO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3097"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFF05_01340"
     CDS             complement(<1..648)
                     /locus_tag="VFF05_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50479.1"
                     /translation="MEAHVAHSIEAFGREEWNALFPDELEDWSYYRAVENSRLPEFSW
                     LYFGVRDETGSLCAAVPAFVTDYRLDTTLSGALKRFADWLTRHFPRLLRQKMLSLGSP
                     VSEVCQLGFAPGTTIDEQRLLLGEILAQAEVFARENRVRMIAVKDASVAQDALWATAA
                     KAHRLRRQPGLPTAFLDVNFANIDAYLARLGKATRKDIRRKLKNATGLRMQWRNDI"
     gene            complement(648..1508)
                     /locus_tag="VFF05_01345"
     CDS             complement(648..1508)
                     /locus_tag="VFF05_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW50480.1"
                     /translation="MIKNVEFHMRGGRNGVLLIHGLTGTPTEMRFIGKGLNRNGFTVY
                     GMQLAGHCGDEADLLATGRSDWYRSVVAAAEKLRGDVDRLFVAGLSMGALLALKLAAD
                     RPEMVDGLGLYGTTFAYDGWTIPWIGKLSFLLPLVTSLGFGRNRKFHECFPYGIKDER
                     IRQRIAGSMLSGDSAAAGLPGNPWPSLAEFYRLSWRVRRMLPNVRTPALVVHAKDDDV
                     ASLKNVRTVVRGVSGPVETVLLENSYHMITVDQERDLVIERTTRFFNEVAATRFVTMP
                     EHAPAMAVAD"
     gene            complement(1564..1932)
                     /locus_tag="VFF05_01350"
     CDS             complement(1564..1932)
                     /locus_tag="VFF05_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZW50481.1"
                     /translation="MLILALLLGTVLCDVGGQVCFKLGVGHESEPVPAGGLRAFFGGL
                     LVSPWIVAGILVYVVEITLWFAALSMTRLSVAYPFMALSYCGVVVASRFILGERVSLR
                     RWLATGAVAFGVALACWPGI"
     gene            complement(1926..>2375)
                     /locus_tag="VFF05_01355"
     CDS             complement(1926..>2375)
                     /locus_tag="VFF05_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50482.1"
                     /translation="WEHAPSRVWRRIADGPGRRWMDGHIHWQNLAQTGLETALDAIIP
                     LISTDAVWLSIDKDVLDESEALTNWDQGRMPLSALLHIVTAVGAHKRIVGADICGEFS
                     PIAHTNWFKRRESRSDQPLRSADASMLARNEATNKELLVAIEKAARC"
BASE COUNT          433 a          767 c          778 g          397 t
ORIGIN      
        1 gatgtcgttg cgccattgca tgcgcaatcc ggtcgcattc ttgagcttgc ggcggatgtc
       61 cttgcgcgtg gccttgccca agcgggcaag gtatgcgtcg atgttggcga agttcacgtc
      121 gagaaatgcc gttggcaaac ccggttggcg gcgcaggcgg tgcgccttcg ccgcggtggc
      181 ccacagtgcg tcctgggcca cgctggcgtc cttgaccgcg atcatgcgca cgcggttttc
      241 gcgcgcgaaa acttcggcct gcgcgaggat ttcgccgagc aggagccgct gctcgtcgat
      301 tgtcgtgccg ggcgcgaaac cgagttggca gacctccgaa accggcgagc cgagggagag
      361 catcttctgg cgcagcaggc gcggaaaatg ccgggtcagc cagtcggcga aacgcttgag
      421 cgcaccgctg agcgtggtgt cgagccggta gtcggtgacg aacgcgggaa cggcagcgca
      481 cagcgatccg gtttcgtcgc gtacgccgaa atacagccag gaaaattctg gcagacggga
      541 attctccacc gcgcggtaat agctccagtc ttccagttcg tccggaaaca aggcgttcca
      601 ttcctcgcgg ccgaaagcct cgatggaatg cgcaacgtgc gcttccatca atcggcgacg
      661 gccatcgccg gcgcgtgctc gggcatggtc acgaaacgcg ttgcggcgac ttcgttgaag
      721 aagcgcgtgg tgcgctcgat gacgaggtcg cgctcctggt ccacggtgat catgtggtag
      781 gaattttcca gcagcacggt ctcgactgga ccactgacgc cgcgcacgac ggtgcgcacg
      841 ttcttcaggc tggccacgtc gtcgtccttg gcatggacga cgagcgccgg cgtgcgcacg
      901 ttcggcaaca tgcgccgcac gcgccaactc aggcggtaga actcggcgag cgacggccac
      961 ggattgccgg gcaggccggc ggcggcgctg tcgccggaca acatgctgcc ggcgatgcgc
     1021 tggcggatgc gctcgtcctt gatgccgtag gggaagcact cgtggaattt ccgattgcga
     1081 ccgaagccca ggctcgtcac tagtggcagc aggaaggaca gcttgccgat ccacggaatc
     1141 gtccagccgt cgtaggcgaa cgtggtgccg tacaagccga ggccatcgac catctccggg
     1201 cgatcggcgg cgagcttgag cgcgagcagg gcgcccatcg acaggccggc cacgaacagg
     1261 cgatcgacat cgccgcgcaa tttctcggcg gcggcgacca cgctgcggta ccagtcgctg
     1321 cggccggtgg cgagcaggtc ggcttcgtcg ccgcaatggc ccgccagctg catgccgtag
     1381 acggtgaaac cgttgcggtt caggcccttg ccgatgaagc gcatctcggt cggcgtgccg
     1441 gtgaggccgt ggatcagcag cacgccattg cggccgcccc gcatgtggaa ttcgacgttc
     1501 ttgatcatgt actgtctcgt aaccttgtgc cgcaattatc cgactgattg gtttacgaat
     1561 accttaaatg ccgggccaac aggccagggc cacgccgaac gccaccgcgc cggtcgccag
     1621 ccagcgccgc aaggaaacgc gctcgccaag gatgaaacgg ctcgcgacca cgacgccgca
     1681 atacgacagc gccatgaagg gatacgcgac gctcagccgc gtcatgctca acgccgcgaa
     1741 ccacagcgtg atttcgacca catacacgag aattcccgcg acgatccacg gtgaaaccag
     1801 caggccgccg aagaatgcgc gcaggccgcc ggcgggcacc ggttcggatt cgtgaccgac
     1861 gccgagcttg aaacacacct ggccgccgac atcgcacagc acggtgccga gcaacagcgc
     1921 caggatcagc atcgtgcggc cttctcgatt gccaccagca gttccttgtt cgtcgcctcg
     1981 ttgcgcgcca gcatcgacgc atcggcgctg cgcagcggtt ggtcggagcg cgattcccgg
     2041 cgcttgaacc agttcgtgtg cgcaatgggg gaaaactcgc cgcagatgtc cgcgccgacg
     2101 atgcgcttgt gcgcgccgac tgccgtgacg atgtgcagca aggccgacag cggcatgcgg
     2161 ccctgatccc agttcgtcag cgcctcgctc tcatcgagca cgtccttgtc gatgctcagc
     2221 cagacagcat cggtcgaaat cagcggaatt atagcatcca gcgccgtttc gaggccggtc
     2281 tgcgccaggt tttgccagtg gatatggcca tccatccagc gccggccggg gccgtccgcg
     2341 atgcgccgcc agacccgcga cggcgcgtgc tccca
//
LOCUS       DASRVO010000118         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_289082, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000118 DASRVO010000000
VERSION     DASRVO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289082"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1501
                     /locus_tag="VFF05_01360"
     CDS             107..1501
                     /locus_tag="VFF05_01360"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZW50483.1"
                     /translation="MSIRLRIPVFGAVFIALAAIALWTGLRPEIGAAGTLPVAVDGQA
                     LPSLAPMLAKVTPAVVNINSKTRVRVRNPLMEDPFFRQFFGMPDMPRERIEQSLGSGV
                     IIDAAKGYVLTNNHVIAGADDIAVTLHDGRTLKAQVVGTDPDTDIAVIRIPAEKLSAL
                     ALADSSQLRVGDFVVALGDPFGLGQTVTSGIVSALNRSGLRGLGYQNFIQTDASINPG
                     NSGGALVNLRGELVGINSAIYSPSGGNVGIGFAIPSNLASRVMRQLIATGTVAHGSLG
                     LAAQDISPEIAAALGVNAGSGALITRVRGGSPAAAAGVAPGDVVTAIDGKPVRSAADL
                     ANAQGILPVGANVDLDLLRDGKTLRVKTRMDADRRASAEGAGVDARLRGAQLSEADPG
                     ARRDGLAGVRVDRVTGGSRAQDSGLRPGDLIVGVNQVDISNLNDLRGLLARSPRQVLL
                     SVVRGRDALFVPLR"
     gene            1582..>2510
                     /locus_tag="VFF05_01365"
     CDS             1582..>2510
                     /locus_tag="VFF05_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HZW50484.1"
                     /translation="MTIRANARRAGLISALFACGMVFAADPAKHHVQVMAESKSVPAD
                     SIVLRGDHATTRLYEALVEAFEKAGDGRVAVQPFSTVSGLDALHAGTADVAASARAAM
                     PDRAQEQGTNFYPVAWDALVPIVAPSNPVSGITLKQLHDVYLGRITNWKDLGGNDAPI
                     NLDGVAPPLDGVEYSTRLLLFHYGDQNVSVSRMYVNTEKLEEDIALNANGLGMSTLAG
                     VARNAKVKMLGVEGVHASTATIADGSYPLYSAIYLATRNGGPRSAEAEKFVAFADSDA
                     GKAILRQEGLVPYADAPDLTTTKQEARIAFVDA"
BASE COUNT          422 a          829 c          815 g          444 t
ORIGIN      
        1 aatcaaacca taagcgctgc ctatggtctg cgcgacgcca gcgaaaccgt tgccggaaaa
       61 agtggtctgt ttccccgcca attcattcgt tttcgaggat ttcaccatgt cgattcgcct
      121 gcgtattccc gtgttcggag ccgttttcat cgctttggcg gctattgcgc tttggacagg
      181 cctgcgcccg gaaatcggcg cggccggcac cttgccggtc gcggtcgacg gccaggcgct
      241 gccctcgctc gcaccgatgc tggccaaggt cacgccggcc gtggtcaaca tcaattccaa
      301 gacccgcgtg cgcgtgcgca acccgttgat ggaggatccg ttctttcggc agtttttcgg
      361 catgcccgac atgccacgcg agcgcatcga gcagtccctg ggttcgggcg tcatcatcga
      421 cgccgcgaaa ggctacgtgc tcaccaacaa ccatgtgatc gccggcgctg acgacatcgc
      481 cgtgaccctg cacgacggac gtacgctcaa ggcgcaggtc gtgggcacgg atccggatac
      541 cgacatcgcc gtgatccgca tcccggcgga aaagctgagt gcgctggcgc tcgccgattc
      601 cagccaattg cgcgtgggcg atttcgtcgt cgcgctgggc gatccgttcg gccttggcca
      661 gacggtgacg tccggcatcg tctcggcgct caaccgcagc ggcctgcgcg gcctcggtta
      721 ccagaacttc atccagaccg acgcctcgat caatcccggc aattccggcg gcgcgttggt
      781 caacttgcgc ggcgaactgg tcggcatcaa ctccgcgatc tattcgccgt ctggcggcaa
      841 cgtcggcatc ggtttcgcga ttccttcgaa tctggccagt cgggtcatgc gccagctcat
      901 cgccaccggc acggtcgcgc atggttcgct cggccttgcg gctcaggaca tctcgccgga
      961 gattgccgcc gccctcggcg tgaatgccgg cagcggcgcg ctgatcacgc gcgtgcgcgg
     1021 cggttcgccg gccgcggcag cgggcgtcgc gcccggcgac gtggtgaccg caatcgatgg
     1081 caaaccggtg cgcagcgcgg cggacctcgc caatgcgcag ggcatcctgc cggtcggcgc
     1141 gaacgtcgac ctcgacctgc tgcgcgatgg caagactctg cgagtgaaaa cccggatgga
     1201 tgcggacagg cgtgccagcg ccgagggcgc cggcgtcgac gcgcgtttgc gcggcgcgca
     1261 attgtccgaa gccgacccgg gtgcgcgtcg cgacggtctt gccggcgtac gcgtggaccg
     1321 cgtgaccggc ggcagccgcg cacaggacag cggtctcagg ccgggcgatc tgatcgtcgg
     1381 cgtcaaccag gtcgacatct cgaacctgaa cgacctgcgt ggattgctcg cacgttctcc
     1441 gcgtcaggta ttgctgtcgg tggtgcgggg tcgggatgcg ctgttcgtcc cgttacgctg
     1501 agtattccgg cactataatc cgcgcaggcg ccacgccgtc gattcgcggt gtgctgcgcg
     1561 catcagcaga ggaattcgat tgtgacgata cgcgccaacg cccgccgtgc cggcctgatt
     1621 tccgccctgt tcgcctgcgg catggtcttc gccgccgatc cggccaaaca ccacgtgcag
     1681 gtcatggccg agtcgaagtc ggtacccgcc gacagtatcg tcctgcgcgg cgaccacgcc
     1741 accacgcgac tctacgaagc cctggtcgag gcgttcgaga aggccggcga cggtcgcgtt
     1801 gcggtacagc cgttcagcac ggtatccgga ctggatgcgt tgcatgccgg taccgcagac
     1861 gtcgccgcca gtgcgcgcgc ggccatgccg gatcgcgccc aggaacaggg cacgaatttc
     1921 tatccggtgg cctgggatgc gctggtgccg atcgttgcgc cgtccaatcc ggtgagcggc
     1981 atcacgctca aacaattgca cgacgtgtat ctgggccgga tcacgaactg gaaggacctc
     2041 ggcggcaacg atgcgccgat caatctcgac ggcgtcgccc cgccgctgga tggcgtggag
     2101 tattccacgc gtctgctgct gttccattac ggcgaccaga atgtttccgt gtcgcgcatg
     2161 tacgtgaata cggaaaagct cgaagaggac atcgccctga acgcgaacgg cctgggcatg
     2221 agcacgctgg ccggcgtggc gcgcaacgca aaagtgaaaa tgctcggcgt ggaaggcgtg
     2281 cacgcttcca cggcgacaat cgcggacggc agctatcccc tgtattcggc gatctacctc
     2341 gccacacgca atggcggccc gcgcagtgcc gaagcggaaa agttcgtggc cttcgcggac
     2401 agcgacgccg gcaaggcgat cctgcgccag gaaggactgg tgccgtacgc ggatgctccc
     2461 gatctgacaa cgaccaagca ggaagcccgc atcgccttcg tcgatgcgca
//
LOCUS       DASRVO010000119         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_81180, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000119 DASRVO010000000
VERSION     DASRVO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81180"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /gene="sucD"
                     /locus_tag="VFF05_01370"
     CDS             <1..814
                     /gene="sucD"
                     /locus_tag="VFF05_01370"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZW50485.1"
                     /translation="GTFHSEQALDYGTKLVGGVTPGKGGGEHIGLPVFNTVQDAVDET
                     GADASMFFVPPPFAADAIMEAADAGIRVIVAITEGIPVLDMLRVKNVLKQYPDTVLIG
                     PNCPGVITPGQCKIGIMPGHIHKPGKIGIVSRSGTLTYEAVFQTTNEGLGQSTVIGIG
                     GDPINGLNFIDCLKLFQDDAQTQGIIMVGEIGGSAEEDAAEFIAEHVTKPVVSFIAGA
                     SAPPGKRMGHAGAIISGGKGTAAAKVAALEKAGVTTVKSPADLGKAVAARLK"
     gene            882..2495
                     /locus_tag="VFF05_01375"
     CDS             882..2495
                     /locus_tag="VFF05_01375"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZW50486.1"
                     /translation="MATLRFALAQFDFPVGAIARNGARVRELMRQARDEGAQLVAFPE
                     LTLSGYPPEDLLLRPSFLATCELELAQVAQAADGIAAFVGHPHALGEVYNAASLLAGG
                     KTQFTAHKQALPNYTVFDEKRYFRPGHDSVVTDFDGVRVGWLVCEDIWEPEPAARAAA
                     QGAELLVVINASPFDVGKASAREELLARRACENRVAIAYLNLVGGQDDLVFDGGSLLV
                     EADGTIAARAPAFEDALLVAEYDSTARKFRALDWPVEADTSREARVYAAIVRGIRDYV
                     GKNHFPGVLLGLSGGIDSALTLALAVDALGADKVMAVRLPSRYTLGLSNDLAAAQAHA
                     QGVQMLTIPIAAPFAGFLDALAPAFAGRAADVTEENLQSRCRGAILMALSNKSGRMLL
                     TTGNKSEYATGYATIYGDMCGGFAPLKDCYKELVYALSRWRNRDGEVIPRAVIERAPS
                     AELRENQTDQDSLPPYEILDAILQRFVEGEQSQSEIVSAGFDEATVRRVARLVLGSEY
                     KRRQAAPGPKISTRAFGRDRRYPISSGWG"
     gene            complement(2505..>2940)
                     /gene="bamD"
                     /locus_tag="VFF05_01380"
     CDS             complement(2505..>2940)
                     /gene="bamD"
                     /locus_tag="VFF05_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZW50487.1"
                     /translation="LDRYANQDMTKRDQAHTLQSFEDFNDLLSRFPNSRYADDARQRM
                     VYLLDNLARGQLNIAKFYLREGAYVAAANRAKDIIEKYQRTPAVGDALAVQVIAYKKL
                     GQDKLAADAERVLKLNYPNHPYFAGNWPKHPHWWWRMIPFRG"
BASE COUNT          510 a          912 c         1008 g          510 t
ORIGIN      
        1 gggcaccttc cattccgaac aggcgctgga ctacggcacc aagctggtcg gcggtgtcac
       61 gcccggcaag ggcggcggcg aacacatcgg cctgccggtg ttcaacaccg tgcaggacgc
      121 cgtcgacgaa accggcgcgg acgcgagcat gttcttcgtg ccgccgccgt tcgcggccga
      181 cgcgatcatg gaagccgccg atgccggcat ccgcgtgatc gtggcgatta ccgaaggcat
      241 accggtgctg gacatgctgc gcgtgaagaa cgtgctcaag cagtatccgg acaccgtgct
      301 gatcgggccg aactgccccg gcgtcattac gccgggacag tgcaagatcg gcatcatgcc
      361 cggccacatc cacaagccgg gcaagatcgg catcgtttcg cgctcgggca cgctcaccta
      421 cgaggccgtg ttccagacca cgaatgaagg actcggacaa tcgaccgtca tcggcatcgg
      481 cggcgatccg atcaatggcc tgaacttcat cgattgcctg aaattgttcc aggacgatgc
      541 gcagacccaa ggcatcatca tggtcggcga gatcggcggc agcgcagagg aagacgcggc
      601 cgagttcatc gccgaacacg tgactaagcc ggtggtgtcc ttcatcgccg gcgccagcgc
      661 gcctccgggc aagcgcatgg gccacgccgg cgcgatcatc tccggcggca agggcacggc
      721 cgcggccaag gtcgcggcgc tggaaaaagc cggtgtgacg acggtgaaat cgccggcaga
      781 tttgggcaag gccgtagccg ctcggttgaa gtaagcggat tggaatcggc gataatcgaa
      841 ggccgcgatt catcgcggcc tttttgcttc cggaaatccg catggcgacc ttgcgtttcg
      901 cgctggccca attcgacttc ccggtcggcg cgatcgcgcg caacggcgcg cgcgtgcgcg
      961 aactgatgcg gcaggcgcgt gacgagggcg cgcagctcgt cgcattcccc gaactgaccc
     1021 tgtccggcta tccacccgag gacctgctgc tgcggccgag ttttctcgcc acctgcgagc
     1081 tcgagttggc gcaggtcgcg caggcggcag acggcatcgc ggccttcgtt ggccatccgc
     1141 atgcgctggg tgaagtatac aatgcggcat cgctgctggc cggcggcaaa acgcaattca
     1201 ccgcgcacaa gcaggcgttg ccgaactaca cggtgttcga cgagaagcgt tacttccgcc
     1261 ctggccatga ttcggtagtg accgacttcg atggtgtgcg cgtgggctgg ctggtctgcg
     1321 aagacatctg ggaaccggaa ccggcagcgc gtgcggcggc gcaaggcgcc gagctgctcg
     1381 ttgtcatcaa tgcttcgccg ttcgatgtcg gcaaggccag tgcgcgcgaa gaattgctgg
     1441 cgcggcgcgc gtgcgagaac cgcgtcgcga tcgcctacct gaatctcgtc ggtggacagg
     1501 acgatctggt cttcgatggc ggatcgctgc tggtcgaggc ggacggaacc atcgccgcac
     1561 gcgcgccggc gttcgaagat gcgctactag tcgccgagta cgacagcact gcgcgcaagt
     1621 tccgcgcgct ggattggccg gtcgaggccg acacgtcgcg cgaggcgcgc gtgtatgcgg
     1681 cgatcgtgcg cggcatccgc gattacgtcg gcaagaatca tttccccggc gttctgcttg
     1741 ggctttccgg cggcatcgac tcggcgctca cgctggcgtt ggcggtcgac gcgctcggcg
     1801 cggacaaggt gatggcggtg cggctgccgt cgcgctacac attgggtttg agcaacgatc
     1861 tggctgccgc gcaggcgcac gcgcagggcg tgcagatgct gacgatcccg attgccgcgc
     1921 cgttcgcggg gtttctcgat gcactcgcgc ccgccttcgc cggccgcgcc gccgacgtta
     1981 ccgaggagaa tctgcaatcg cgctgccgcg gcgcgattct gatggcgctg tcgaacaaga
     2041 gcggacgcat gctgctcacg accggcaaca aaagtgaata cgcgaccggg tacgcgacga
     2101 tctacggcga catgtgcggc ggcttcgccc cgctcaagga ttgctacaag gaactcgtct
     2161 acgcgctctc gcgctggcgc aaccgcgatg gcgaagtcat cccgcgggcc gtgatcgaac
     2221 gtgcgccgtc ggcggaactg cgcgagaacc agaccgacca ggattcgctg ccgccgtacg
     2281 aaatcctcga cgcgatcctg cagcgcttcg tcgaaggcga acaatcgcag tccgaaatcg
     2341 tcagcgcagg attcgacgag gccacggtgc gccgcgtcgc gcgtctggtc ctgggcagcg
     2401 aatacaagcg ccgccaggct gcgccggggc cgaagatttc cacgcgcgca ttcggccgcg
     2461 accggcgcta tccgatcagt tcggggtggg gttagggaat cgcgttaacc gcggaacgga
     2521 atcatccgcc accaccagtg cggatgcttg ggccagttgc cggcgaagta ggggtggttc
     2581 ggatagttga gcttgagcac gcgctcggcg tcggcggcga gtttgtcctg cccgagtttc
     2641 ttgtaggcga tgacctggac cgccaacgcg tcgccgaccg cgggcgtgcg ctggtatttt
     2701 tcgatgatgt ccttggcccg gttcgcggcg gcaacgtagg cgccttcgcg caggtagaac
     2761 ttggcgatgt tgagctggcc gcgcgcgaga ttgtcgagca gatagaccat gcgctggcgc
     2821 gcgtcgtcgg cgtagcggct gttcggaaaa cgcgagagca ggtcgttgaa atcctcaaac
     2881 gattgcaggg tgtgggcttg gtcgcgcttg gtcatgtcct ggttggcgta gcggtcgagg
//
LOCUS       DASRVO010000120         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_133065, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000120 DASRVO010000000
VERSION     DASRVO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133065"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1090)
                     /locus_tag="VFF05_01385"
     CDS             complement(<1..>1090)
                     /locus_tag="VFF05_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HZW50488.1"
                     /translation="SAHTAIDIVTDDMPFLVDSVGIAVAQAGLHVHLVAHPVYAVERD
                     AGGNVLAFAVEGGKGKPESLMHFEIDRIADPAERARLEQSVRAALDDVAQCYRDWPAM
                     RDRMLAIADGLASSKMPVDAAGVAEAQEFLRWAAADHFTFLGYREYRVGQSGSEEVLL
                     AVDGSGLGILRGSERSVAPRSLKSLAARDLPQSGAIDAIILTKTNARSSVHRSGYMDY
                     IGVIEFDAHGIPVAERRFIGLFTSSAYARRPWEIPLVRHKFEVVMERSGLRRDSHSGK
                     NLRHILETLPRDELFQASEDELFATATGVLGLQGRARARLFVRRDKYGRFFSCMVFMP
                     RDRFTADVRERIEALLRDTFHGERLDSTI"
BASE COUNT          205 a          369 c          351 g          165 t
ORIGIN      
        1 gatcgtggaa tccagacgct cgccgtggaa cgtgtccctg agcagagcct cgatgcgctc
       61 gcgcacgtcg gcggtgaagc gatcgcgcgg catgaacacc atgcaggaaa agaaacggcc
      121 gtatttgtcg cggcgcacga acaggcgcgc gcgcgcgcgg ccctgcaggc cgagcacgcc
      181 ggtggcggtg gcgaacaact cgtcctcgct ggcctggaac agctcgtcac gcggcagggt
      241 ctcgagaata tggcgcaggt tcttgcccga atgcgaatcg cgacgcagtc ccgagcgctc
      301 catcaccacc tcgaacttgt gccgcaccag cggaatctcc cagggccgac gcgcatacgc
      361 actcgatgtg aacaggccaa taaagcgccg ctcggccacc ggtatgccgt gcgcgtcgaa
      421 ttcgatcacg ccgatgtaat ccatgtagcc cgaacgatgc acgctggagc gggcattggt
      481 cttggtcagg atgatcgcgt cgatcgcgcc ggattgcggc aggtcgcgcg cggccagcga
      541 cttgagcgag cgcggcgcca ccgaacgctc gctgccgcgc aggatgccga gcccggagcc
      601 gtccacggcg agcaggactt cttcgctgcc ggattggccg acccgatact cgcggtagcc
      661 caggaacgtg aaatgatccg ccgccgccca acgcaagaat tcctgcgctt cggcaacacc
      721 ggccgcatcc accggcattt tgctggacgc cagtccgtcg gcgatcgcca gcatcctgtc
      781 gcgcatcgcc ggccagtcgc ggtaacactg ggcgacatcg tccagcgccg cacgcacgga
      841 ctgctccagg cgcgcacgct ccgccgggtc ggcgatgcga tcgatttcga aatgcatcag
      901 cgactcgggc ttgcccttgc cgccttccac cgcaaaggcg agcacattgc cgccggcatc
      961 gcgttcgacg gcgtaaaccg gatgcgcgac caggtgcacg tgcaaaccgg cctgagccac
     1021 ggcgatgccg accgagtcga ccagaaacgg catgtcgtcg gtgacgatgt caatggcggt
     1081 atgcgcggat
//
LOCUS       DASRVO010000121         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_340970, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000121 DASRVO010000000
VERSION     DASRVO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340970"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFF05_01390"
     CDS             <1..801
                     /locus_tag="VFF05_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50489.1"
                     /translation="IGLLLGHYGQWRELGLDRVRALAAMYALGSLPLLDVHVALAGYA
                     DLWLAAMLGFAVLAWLRWRRERDHGQLAIAIVCVALLPAIKLEGAIWLLLCAAVAIYD
                     VLPRRWRRLTLSGAIVVLLVLAFVSRIDLPVPGLGWVEITHGRIVVPALGDYAMHFNA
                     DALSSLLNSLFVQPNWHLLWWLAPGIALWHWRALRDDAALRHLAAFVLLGFAFLIFLF
                     TCTDASRWAESYTAVNRLILQLVPATITALALICRDRAPRSAASPAPA"
     gene            complement(734..>1032)
                     /locus_tag="VFF05_01395"
     CDS             complement(734..>1032)
                     /locus_tag="VFF05_01395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW50490.1"
                     /translation="ESFGIVLLEAMRAGLPAIASDIPGSGVGFVVRDGETGLLVAPGD
                     VDALVATIRKLAGDADSRRRLGAAGRRRFEREFTLERVMPPIAALYRDISKPAP"
BASE COUNT          139 a          342 c          350 g          201 t
ORIGIN      
        1 ataggcctgt tgctcggaca ctacgggcag tggcgtgagt tgggactgga tcgcgtgcgc
       61 gccctggcgg cgatgtatgc gctcggctcg ctgccgctgc tcgacgtgca tgtcgccctg
      121 gccggttacg cggatttgtg gctggcggcc atgcttggct tcgccgtgct ggcctggctg
      181 cgctggcggc gcgaacgcga tcatggacaa ctcgccatcg ccatcgtctg cgtggcattg
      241 ttgccggcga taaaactcga aggtgcaatc tggctgctgc tgtgcgcggc cgtcgcgatt
      301 tacgacgtcc tgccgcggcg ctggcggcgc ctgaccctgt ctggcgcgat cgtcgtgctg
      361 ttggtgctgg ctttcgtcag ccgcatcgat ctgccggtgc cgggattggg ttgggtggaa
      421 atcacacatg gccgcatcgt cgtgcccgcg ctcggcgact acgcgatgca cttcaatgcc
      481 gacgcgctgt cgagtctgtt gaacagcctg ttcgtgcagc cgaactggca cctgctgtgg
      541 tggctggcgc cgggcatcgc gctgtggcac tggcgcgcgc tgcgcgacga cgcggcgctg
      601 cgccatctcg ccgcgttcgt gctgctcgga ttcgcgttcc tgatatttct gttcacctgc
      661 accgacgcat cgcgctgggc cgagagctac accgcggtca atcgcctgat cctgcagctg
      721 gtgccggcga cgatcacggc gctggctttg atatgtcgcg atagagcgcc gcgatcggcg
      781 gcatcacccg ctccagcgtg aattcgcgct caaagcgccg acggcccgct gcgccgaggc
      841 ggcggcgcga gtccgcgtcc ccggcgagct tgcggattgt tgccaccaaa gcatcgacgt
      901 cgcccggcgc aaccagcagg ccggtttcgc catcgcgcac gacgaagccc acgcccgatc
      961 cgggaatatc gctggcgatc gccggcaatc ccgcgcgcat ggcttcgagc agcacgatcc
     1021 cgaacgactc gg
//
LOCUS       DASRVO010000122         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_159093, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000122 DASRVO010000000
VERSION     DASRVO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159093"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1081
                     /locus_tag="VFF05_01400"
     CDS             <1..>1081
                     /locus_tag="VFF05_01400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZW50491.1"
                     /translation="ANVLTQYRTNPQQLPPRDVLDHQVLESLIMLRLQVARAKDTGIR
                     VSDADIDQAVQRVAEHNKITVDQLRGSLASEHMSFEDFRNHLRDQLLVQRLQQRVVQG
                     QSNVSDSEIDILLASNSLKSGEVRLRHILIGLPNGADASQIAAARAKADEVKAKIDGG
                     MDFTTAAIQYSSAQDALQGGDLGWRRYDEVPEAFANLVEGMKPGDVSQPLRGPSGFHI
                     VKLVDKRAEGKQVVTEYHARHILIKVDELTSSADAKKTIEDIRRRIVDGHEDFAALAK
                     KDSQDPNSASAGGDMGWFPIDQYGSSVATQLATLKSGEISQPFESDLGWHILQLEGTR
                     EQDRTKEMKREQARDVIRNRKAEQAY"
BASE COUNT          236 a          369 c          329 g          147 t
ORIGIN      
        1 cgccaacgtc ctcacccagt accgcaccaa tccgcagcaa ctgccgccgc gtgacgtgct
       61 cgaccaccag gtgctggaga gcctgatcat gctgcgcctg caggtggcgc gcgccaagga
      121 caccggcatt cgcgtcagcg acgccgacat cgaccaggcc gtgcaacgcg tcgccgagca
      181 caacaagatc accgtggatc agttgcgcgg ctcgctcgcc agcgaacaca tgagtttcga
      241 ggatttccgc aatcacctgc gcgaccagct cctggtgcaa cgcctgcaac agcgggtcgt
      301 gcagggccag tccaacgtca gcgattccga aatcgacatc ctgctcgcca gcaacagcct
      361 caaatccggc gaagtgcgcc tgcgccacat cctgatcggc ctgcccaacg gcgccgatgc
      421 atcgcagatt gccgctgcgc gcgccaaggc cgacgaagtc aaggccaaga tcgacggcgg
      481 catggatttc accaccgcgg cgatccagta ctcgagcgcg caggacgcgc tgcagggcgg
      541 cgacctgggc tggcgccgct acgacgaagt gccggaggcg ttcgcgaatc tggtcgaagg
      601 catgaagccg ggcgacgtgt cgcagccgtt gcgcggaccc agcggcttcc acatcgtcaa
      661 actcgtcgac aagcgcgccg aaggcaagca agtcgtgacc gaataccacg cgcgccacat
      721 cctgatcaag gtcgacgaac tgaccagcag cgccgacgcg aagaagacca tcgaggacat
      781 tcgccgccgc atcgtcgacg gccacgaaga tttcgccgcg ctggccaaga aggattcgca
      841 ggatcccaac tccgccagtg ccggcggcga catgggctgg ttcccgatcg accagtacgg
      901 ttcatcggtg gcgacccagc tcgccacgct caagagcggc gagatttcgc aaccgttcga
      961 gagcgatctg ggctggcata tcctgcaact cgaaggcacg cgcgaacagg accgcaccaa
     1021 ggaaatgaag cgcgaacagg cgcgcgacgt gatccgcaat cgcaaggccg agcaggctta
     1081 c
//
LOCUS       DASRVO010000123         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_159154, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000123 DASRVO010000000
VERSION     DASRVO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159154"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFF05_01405"
     CDS             <1..684
                     /locus_tag="VFF05_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZW50492.1"
                     /translation="GWRFIAFGPDGKLYVPIGAPCNVCDKPGYAKLTRMNADGGGVED
                     VALGIRNTVGFDWQPGTGKLWFTDNGRDWLGDDAPSDELNRADRLREHFGFPYCHQGD
                     LPDPEFGKGHACGDYTPPALKLGAHVAALGMRFYTGTQFPVGYRNSIFIAEHGSWNRS
                     SKVGYRVVNVRVDGERAVEGAPFLSGWLRSGQSAWGRPADVLVMPDGALLVSDDLAGA
                     IYRITWSKP"
     gene            681..1991
                     /locus_tag="VFF05_01410"
     CDS             681..1991
                     /locus_tag="VFF05_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HZW50493.1"
                     /translation="MNANIDVAAAALERARQLGQQGRAEEAAGEYSRVLAAQPEHAEA
                     LAFFGTRELALGRAQQAIEYLQRAARANPDEAQIQLSLGSAYFAARRIDEACSALARC
                     LELEPKAYVARLQYAIALEQIAKSEAALPQYFSAIVQAQREGRWLSDASTAPMLRAQV
                     KHAMRTTFAGRKRLFESLLAPHVQRHGEAAMARVAKCLKIYLGELPPNYPDPRQIPKF
                     LYFPDLPPTTYFDRTLFPWYEILERSWTAIRDELSAVLHEPDALAPFLGEHPPEAMKE
                     YLGGVEKPVWDAYFFYRHGKRFDAHHARCARTSAALESLPTLVRIREHAPEACYSVLT
                     PGTHILKHRGVTNTRLVTHLPLVIPENCAINVGGEEKVWREGEVFSFDDTFEHEAWNR
                     SDRTRVVMLLDVWNPYMTTAECEAITGLIEGIGDFNQSAGIDRE"
BASE COUNT          369 a          644 c          650 g          373 t
ORIGIN      
        1 ggttggcgct tcatcgcgtt cggtccggac ggcaagctct acgtgccgat cggcgcgccg
       61 tgcaatgtct gcgacaagcc cggttacgcc aaactcacgc gcatgaatgc cgatggcggc
      121 ggcgtggaag atgtcgcgct cggtatccgc aacacggtcg gcttcgactg gcagccgggc
      181 acgggaaagc tctggttcac cgacaacggc cgcgactggc tgggcgacga tgcgccatcc
      241 gatgaattga atcgcgccga tcgtctgcgc gagcattttg gttttccata ctgccaccag
      301 ggcgatctgc ccgatccgga attcggcaag ggccatgcgt gcggcgatta cacgccaccg
      361 gcgctcaagc ttggcgcgca cgtcgccgcg ctcggcatgc gtttttacac cggtacgcaa
      421 ttcccggtcg ggtacaggaa ttccatcttc attgccgagc acggttcgtg gaaccgctcg
      481 agcaaggtcg gctatcgtgt cgtgaacgtg cgcgtggacg gcgaacgcgc cgtcgaaggc
      541 gcgcccttcc tgagtggctg gctgcgatcc ggccagagtg cgtggggccg tccggccgat
      601 gtgctggtca tgcccgacgg cgccttgctc gtatccgacg acctcgccgg cgccatttac
      661 cgaatcacgt ggtccaagcc atgaatgcaa acatcgacgt tgccgctgca gcactcgaac
      721 gtgcgcgcca acttggccag cagggacgcg ccgaggaagc ggccggcgaa tactcgcgcg
      781 tgctcgccgc gcaacccgag catgccgagg cgctcgcgtt tttcggcacc cgcgaattgg
      841 cgctgggtcg ggcgcaacag gccatcgaat acctgcagcg tgccgcccgc gcgaatcccg
      901 atgaggcgca gatccagttg agtttgggca gcgcgtattt cgccgcgcgg cggatcgacg
      961 aagcctgttc cgcgctggcg cgttgcctgg agctggaacc gaaggcctac gtggcgcgcc
     1021 tgcaatatgc gatcgcattg gaacaaatcg ccaagtcgga ggcggcgttg ccgcaatatt
     1081 tcagcgccat cgtgcaggcc cagcgcgaag gccgctggct cagcgacgcc agcacggcgc
     1141 cgatgctgcg cgcccaggtc aagcatgcaa tgcgcaccac cttcgcgggg cgcaagcgcc
     1201 tgttcgagtc gttgctcgcg ccgcatgtgc agcgccacgg cgaagcggca atggcgcgtg
     1261 tggccaagtg cctgaaaatc tatcttggcg aactgccgcc gaattacccg gatccgcgcc
     1321 agattccgaa attcctgtat ttcccggatc tgccgccgac cacttatttc gaccgcacgc
     1381 tgtttccatg gtacgaaatt ctggaacgca gctggaccgc aatccgcgac gaattgagcg
     1441 cggtgttgca cgagcccgat gcgctggcgc cattcctcgg cgagcatccg ccggaggcga
     1501 tgaaggagta tctcggtggc gtggaaaagc cggtctggga tgcgtacttt ttctatcgcc
     1561 acggcaagcg cttcgacgcg catcatgcgc gctgtgcgcg cacgagcgcc gcgctggaat
     1621 cgctaccgac cctggtacgc atccgcgaac acgcgcccga agcgtgctat tcggtgctca
     1681 cgcccggcac gcacatcctc aagcatcgtg gcgtgaccaa cacgcgcctg gtcacgcatc
     1741 tgccgctggt cattccggaa aactgcgcga tcaacgtcgg cggcgaggaa aaggtctggc
     1801 gcgagggtga ggttttcagt ttcgacgaca ccttcgagca cgaagcctgg aaccgcagcg
     1861 accgcacccg cgtggtcatg ctgctggacg tgtggaatcc gtacatgacg actgccgaat
     1921 gcgaagcgat caccggactg atcgagggca tcggcgattt caatcagtcg gccggtatcg
     1981 atcgcgagta gcatcatttt ggttcgtatg gccgtgtttc atgcaccaat atggtg
//
LOCUS       DASRVO010000124         3427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_133216, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000124 DASRVO010000000
VERSION     DASRVO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133216"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="der"
                     /locus_tag="VFF05_01415"
     CDS             <1..967
                     /gene="der"
                     /locus_tag="VFF05_01415"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZW50494.1"
                     /translation="SAHGRGVPALVDTVLAGLPQADPDALAAESANDGVRVAIVGRPN
                     VGKSTLVNRLLGEERVVVSDVAGTTRDAIHVRLERDGKRYTLIDTAGVRRRARVEEAV
                     EKFSVIKTLQSIAAANVAVVLLDAHEGVSEQDATLIGHVLDEGRALVIAVNKWDGLST
                     YEREQCRVSLGRKLDFVGYARVVTISALHGSGIAELMKAIDRAHASATREFGTAELTR
                     ALEKAYTSYQPPMVRGHAPKLRFAHPGGTNPPGIIIHGSRTRHVAEGYRRYLENFFRK
                     RFRLEGTPIRIEFRESENPFAGRKNELTEGQKRKRQRMIRKAKKR"
     gene            971..1567
                     /gene="mobA"
                     /locus_tag="VFF05_01420"
     CDS             971..1567
                     /gene="mobA"
                     /locus_tag="VFF05_01420"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR02665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HZW50495.1"
                     /translation="MPASDPAIITAAILAGGEGRRVGGEDKGLLPLAGRPLIAHVTAA
                     LRGQVGCIVICANRNADRYSEFAPVIADGEDGFRGPLAGIAAALRVCESEWLLTVPVD
                     CPRPPRDLAVRLHDAALAESRDVVAVFDGTRVQPLFALYRRTLAADAEVALKNDMPVI
                     RWQQALHRALADFSTRAQEFGNLNTADEFRQWELGADG"
     gene            1560..2804
                     /gene="glp"
                     /locus_tag="VFF05_01425"
     CDS             1560..2804
                     /gene="glp"
                     /locus_tag="VFF05_01425"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HZW50496.1"
                     /translation="MAEVDDIFPTGLSVEQARALIVAIAQARSLPAEIIPLSAAYGRI
                     LANDAIAPLDVPGFANSAMDGFALRAADLPASGEKRLRLSGEIFAGGSVVPEVETDSC
                     VRITTGAPLPPGADTVVMKENAHVDGDHVVVGAGTRLGANVRPAGEDYRAGDTALKRG
                     CRLGPAQLGVLASFGMDQVPVAKRPKAVLLTTGDELVAPGDALGFGQIHDSNRYSLGS
                     LLESLGAELVRHERVRDDRVALRDALQRAAADADLIVSSGGVSAGEADFLPRLVGEIG
                     KVHFWKVRIKPGMPFLFGQVGSALFFGLPGNPVSGFATCLTLLRPALDALTGTAPAQV
                     SLRARLRASIAKRHARAEFMRARLACDEQAVLWATPHAKQGSGMLRGVADANVLILLA
                     EGERVFAVGEVVEVLSLPGWPE"
     gene            2823..>3427
                     /locus_tag="VFF05_01430"
     CDS             2823..>3427
                     /locus_tag="VFF05_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZW50497.1"
                     /translation="MEIECITPAEALRRQHGGAVLIDVREDDERAAGKPAGAIGVPLG
                     ELAERIGSIVPDRAATIALSCAHGQRSLYAAALLRQMGYERLASVDGGFVRWTAEGLP
                     VERGAFDADAAERYSRHLLLPEVGASGQQRLLQSRVALIGAGGLGSPAGLYLAAAGVG
                     TLTLIDDDVVEKSNLQRQVLHTDARVGQSKTESARIALNAL"
BASE COUNT          569 a         1111 c         1156 g          591 t
ORIGIN      
        1 gtccgcgcac ggacgcggcg tgccggcgtt ggtcgacacc gtgctggcgg gcttgccgca
       61 ggccgatccc gatgcgctgg ccgcggagag cgccaacgac ggcgtgcgcg tggccattgt
      121 cggccgcccg aacgtcggca agtccaccct cgtcaaccgc ctgctcggcg aggagcgcgt
      181 ggtcgtatcc gatgtcgccg gcaccacgcg tgacgcaatc catgtccggc tcgagcgcga
      241 cggcaagcgc tacaccctga tcgacacggc cggcgtgcgc cgccgcgcgc gcgtcgaaga
      301 agcggtggag aaattcagcg tcatcaagac cctgcaatcc atcgccgcgg caaacgtcgc
      361 cgtggttttg ctggacgcgc acgaaggcgt ttccgaacag gacgcaacct tgatcgggca
      421 cgtcctcgac gaaggccgcg cgctggtgat cgcggtcaac aagtgggatg gactttccac
      481 ctacgaacgc gagcaatgcc gcgtctcgct cgggcgcaag ctcgacttcg tcggctacgc
      541 gcgcgtggtg acgatctccg ccctgcacgg cagcggcatc gccgaactga tgaaggccat
      601 cgaccgcgca cacgcttcgg cgacgcgcga attcggaacg gccgagctca cgcgcgcgct
      661 ggaaaaagcc tacaccagct accaaccgcc gatggtgcgc gggcatgcgc cgaaactgcg
      721 ctttgctcac ccgggcggca ccaacccacc gggcatcatc atccacggca gccgcactcg
      781 gcatgtcgcc gaaggctacc gacgctacct cgagaatttt ttccgcaaac gtttccgcct
      841 cgaaggcacg ccgatccgca tcgaattccg cgaaagcgaa aatcctttcg ccgggcgcaa
      901 gaacgaactg accgaaggcc agaaacgcaa gcgccagcgc atgatccgca aagcgaaaaa
      961 acgctgagcc gtgcccgcgt ccgatccagc gatcatcact gcggccattc tcgccggcgg
     1021 ggaagggcgc cgggtcggcg gcgaggacaa gggcctgttg ccgctggccg gacgtccgct
     1081 gatcgcgcac gtgaccgccg cgctgcgcgg gcaggtcggt tgcatcgtga tttgcgccaa
     1141 ccgcaacgcc gatcgttatt cggaatttgc gcccgtgatt gccgatggcg aggatggctt
     1201 tcgcggccct cttgcgggca tcgctgctgc gctgcgtgtg tgcgaaagcg aatggttgct
     1261 taccgttccg gtggattgcc cgcggccgcc gcgcgatctc gccgtgcgtc tgcacgatgc
     1321 ggcgctggct gaaagccgcg acgtcgttgc agtcttcgac ggtacgcgcg tgcaaccgtt
     1381 gttcgcgttg tatcggcgca ctctggccgc cgatgccgag gtagcgttga aaaacgacat
     1441 gccggtgatc cgctggcaac aggctctgca tcgtgcgctt gccgattttt cgacacgagc
     1501 gcaggaattc ggcaatctca acaccgcgga tgaatttcgg cagtgggagc tgggcgcaga
     1561 tggctgaagt agacgatatt tttccgaccg gactgagcgt cgagcaggcg cgcgcgctca
     1621 tcgtggcgat cgcgcaggcg cgcagcttgc ccgccgaaat cattccgctg tcggccgcat
     1681 atggccggat cctggcgaac gatgccattg cgccactcga tgtgcccggc ttcgccaatt
     1741 cggcaatgga tggattcgcg ttgcgcgccg ccgacctgcc cgcatcgggc gaaaagcgct
     1801 tgcgcctgag cggcgagatt tttgccggcg gtagcgtggt tccagaagtc gaaacggatt
     1861 cgtgcgtgcg catcaccacc ggcgcgccat tgccgccggg cgccgatacc gtggtgatga
     1921 aagagaacgc gcatgtcgac ggcgatcatg tcgtcgtcgg cgccggcaca cggcttggcg
     1981 ccaatgtgcg tccggctggg gaggattacc gcgccggcga tactgcgctc aagcgtggat
     2041 gcagactcgg tccggcgcag ctcggcgtgc tggcgtcttt cggcatggac caggttcccg
     2101 ttgcgaagcg cccgaaagcc gtgctgctga ccaccggcga cgagttggtc gcgcccggcg
     2161 atgcgcttgg gttcgggcag attcacgaca gcaatcgcta tagcctgggc agcctgctcg
     2221 aatcgctggg cgctgaactc gtccgccacg agcgcgtgcg cgatgatcgc gtcgcactgc
     2281 gcgatgcgct gcaacgcgca gccgccgatg ccgatctcat cgtaagcagc ggcggcgttt
     2341 ccgcgggcga agcggatttc ctgccgcgcc tcgtcggtga aatcggcaag gtgcatttct
     2401 ggaaagtgcg catcaaaccc ggcatgccgt tcctgttcgg gcaggtcggc tcggcgctgt
     2461 tcttcgggct tccgggcaat cccgtttccg gatttgccac ttgcctgacc ttgctcaggc
     2521 cggcactgga tgcgctgacc ggaaccgcgc cggcgcaggt gtcgctgcgc gcacgattgc
     2581 gcgccagcat tgccaagcgc catgcgcgcg ccgagttcat gcgcgcacgc ctggcctgcg
     2641 acgagcaggc agtcctgtgg gcgacaccgc atgccaaaca gggttccggc atgctgcgcg
     2701 gcgtagccga tgcgaacgtg ctgatcctgc tggcggaagg cgagcgcgta tttgcggtgg
     2761 gcgaagtcgt cgaggtgttg tcgctgcccg gctggcccga atagcgcgcg tataatccgc
     2821 gcatggaaat cgaatgcatc acgcccgccg aggcgctgcg gcgccagcac ggcggggcgg
     2881 tcctgatcga cgtgcgcgag gacgacgagc gcgccgcggg caaacccgca ggtgctatcg
     2941 gcgtgccgct gggcgaactc gccgaacgca ttggatccat cgtgccggat cgcgcggcaa
     3001 ccatcgcgct gagctgcgcg catggccagc gttcgttgta tgccgcggcg ctgttgcggc
     3061 aaatgggtta tgagcgcctc gcttcggtcg acggcggttt cgtgcgttgg acggcagaag
     3121 gcctgccggt ggaacgcggc gcgttcgacg ccgatgcggc agagcgttat tcgcgccact
     3181 tgttgctgcc cgaagtcggc gccagcggcc agcaacgtct tcttcagtcg cgcgtcgccc
     3241 tgattggcgc cggcggcctc ggatcacctg ccgggttgta tctggctgcg gccggcgtgg
     3301 gcacgctgac cctgatcgac gacgatgtcg tcgagaaatc caatttgcag cgccaggtgc
     3361 tgcacaccga tgcgcgcgtg ggccagtcca agaccgaatc ggcgcgcatc gcgctgaatg
     3421 cgttgaa
//
LOCUS       DASRVO010000125         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_55522, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000125 DASRVO010000000
VERSION     DASRVO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55522"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="murG"
                     /locus_tag="VFF05_01435"
     CDS             complement(<1..898)
                     /gene="murG"
                     /locus_tag="VFF05_01435"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZW50498.1"
                     /translation="MNANAPVLIMAGGTGGHIFPGIAVAQELERRRVPVIWLGGKTGL
                     ETRLVPQHGIALETIDISGVRGKGMVTLLRMPFKLAGAVLAARKLIRKHAPRSALALG
                     GYAAAPGGIAARMMKIPLVVHEQNRIPGKTNRLLAKKAVRVLSGFDGVFPGAEWVGNP
                     VRESIAKIAAPEQRYAARDGALKLLVLGGSQGAQSLNTALPEVLRRRGTKLPVAVRHQ
                     CGEAHFDKARNAYLSAGIDAEVVPFEKDMAGAYAWADLVICRSGALTLAELAAAGAPS
                     ILVPFPHAVDDHQTRNAEAMVAA"
     gene            complement(895..2094)
                     /gene="ftsW"
                     /locus_tag="VFF05_01440"
     CDS             complement(895..2094)
                     /gene="ftsW"
                     /locus_tag="VFF05_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HZW50499.1"
                     /translation="MFGFDSQATRRDESRGRYDPVLIVVAIALASLGVVMVASSSIAV
                     ADGQHVGPFHYLFRHLVFLGLGIGLCGAMMKLELEWFERNAFSLLLFAVILLLLVFAP
                     GFGMRINGARRWVRLGIAGFQSVEAVKLLFVFYLASYLVRQHNAVRTRFFGVIKPVGV
                     AVVLLGLLLMQPDFGSAALIIAVTVGMLWLGGARMRNLFYLAIPAMPAIAWAAMTSDY
                     RVKRLTSFLNPWADPFNDGFQLTQALIAVGRGEWFGVGLGASVQKLFYLPEAHTDFIL
                     AVIAEELGFAGIVLVIGLFAILVGRALALGLRAVERGQRYAGYCAFGIGLMIGLQVLV
                     SIGVNLGVLPTKGLTLPLISSGGSSVLMTCAAIGVLLRVSYEVTRALPSDAQIVKPEA
                     LLKGARV"
     gene            complement(2097..>3143)
                     /gene="mraY"
                     /locus_tag="VFF05_01445"
     CDS             complement(2097..>3143)
                     /gene="mraY"
                     /locus_tag="VFF05_01445"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZW50500.1"
                     /translation="ISFIVGPRTIAWLTRMKIGQAVRDDGPQTHLTKAGTPTMGGALI
                     LVSVSVTTLLWGDLANRFVWVVLLVTLGFGAIGWVDDYRKVVYRNPKGLPARSKFFWQ
                     SVIGVVAAVYLAFSVSAPSNSQFLHLFMEWVRSGFSVSLPPQADLIVPFFKTISYPLG
                     LWGFIALTYFVIVGTSNAVNLTDGLDGLAIMPTVMVAGALGVFAYVSGNAVFARYLQF
                     PFIVGAGELAVICGALAGAGLGFLWFNAYPAQVFMGDVGALAVGAALGTVAVIVRQEI
                     VLLIMGGVFVMETASVILQVASFKLTGKRLFRMAPIHHHFELKGWPEPRVIVRFWIIS
                     VVLVLIGLATLKVR"
BASE COUNT          633 a         1035 c         1022 g          453 t
ORIGIN      
        1 cggctgcaac catggcttcg gcgttcctgg tctggtgatc gtccaccgca tgcgggaacg
       61 gaaccaggat cgatggcgcg ccagcggcgg caagttccgc gagcgtgagc gcgccggagc
      121 ggcagatgac caggtccgcc caggcgtagg cgccggccat gtctttctcg aacggcacca
      181 cttcggcgtc gatgccggcg gacagatacg cattgcgtgc cttgtcgaaa tgcgcctcgc
      241 cgcattgatg gcgcactgcg accggcagct tggtgccgcg ccggcgcagc acttctggca
      301 gcgccgtgtt caaactctgc gcgccctgac tgccgccgag cacgagcaat ttcaacgcgc
      361 cgtcgcgggc cgcgtagcgc tgctcgggtg ctgcgatttt tgcgatcgat tcgcgcaccg
      421 gattgccgac ccactcggca cccggaaaga cgccatcgaa tccactgagc acgcgcacgg
      481 cttttttcgc gagcaggcga ttggtcttgc cgggaatgcg gttctgctcg tgcacgacga
      541 gcgggatctt catcatgcgc gcggcgatgc cgccgggcgc ggccgcgtag ccgccgagcg
      601 caagcgcact gcgcggcgcg tgcttgcgaa tcagcttgcg cgcggcaagc actgcgccgg
      661 cgagcttgaa tggcatgcgc agcagcgtga ccatgccttt gccgcgcacg ccggaaatat
      721 cgatcgtttc cagtgcgatg ccgtgttgcg ggacgagacg cgtttccagt ccggtcttgc
      781 cgccgagcca gattaccggc acgcgccggc gctcgagttc ctgggcgacg gcaatgccag
      841 ggaagatgtg gccgccggtg ccgccggcca tgatcaacac gggagcattc gcgttcatac
      901 gcgcgccccc ttcagcaagg cctcgggttt cacgatctgc gcgtcggacg gcagcgcacg
      961 cgtgacctcg taactcacgc gcagcagcac gccgatcgcg gcgcaggtca tgagcacgct
     1021 ggagccgccg gaactgatca gcggcaaggt caggcccttg gtcggcaaca cgccgaggtt
     1081 cacgccgatc gacaccagca cctgcaggcc gatcatcagg cctatcccga acgcgcaata
     1141 accggcgtag cgctggccgc gctcgactgc gcgcaggccg agcgcgagcg cacgcccgac
     1201 caggatcgcg aacaagccga tgacgaggac gatgccggcg aagcccagct cttcggcgat
     1261 cacggcgagg atgaaatcgg tgtgcgcctc gggcaggtag aaaagtttct gcacgctggc
     1321 gccgaggccc acgccgaacc actcgccgcg gcccacggcg atcagcgcct gggtgagctg
     1381 gaacccatcg ttgaacgggt cggcccaggg attgaggaac gaggtcaggc gcttgacccg
     1441 gtaatcgctg gtcatcgccg cccaggcgat tgccggcatg gccgggatcg cgagatagaa
     1501 caaattgcgc atgcgcgcac cgccgagcca gagcatgccg acggtgacgg caatgatcag
     1561 cgccgcgctg ccgaaatccg gttgcatcag cagcaggccg agcaggacca cggccacgcc
     1621 gaccggcttg atcacgccga agaaacgcgt gcgcaccgcg ttgtgctggc gcacaagata
     1681 gctggccaga taaaacacga acagcagctt gaccgcctcg accgactgga atccggcaat
     1741 gcccaatcgc acccagcgcc gcgcgccgtt gatgcgcatg ccgaatcccg gcgcgaacac
     1801 gagcaggagc aggatcacgg cgaacagcag caggctgaag gcgttgcgct caaaccattc
     1861 cagttcgagc ttcatcatcg cgccgcacag gccgatgccg agtccgagaa aaaccagatg
     1921 ccggaacaag taatggaacg gcccgacgtg ctggccgtcg gcgaccgcga tcgaactcga
     1981 cgccaccatc accacgccca gactcgccag cgcgatggcg acgacgatca gcacgggatc
     2041 gtagcgtccc ctgctctcgt cgcggcgtgt cgcttgtgag tcgaagccga acattctcag
     2101 cgtaccttca acgtggcgag gccaatcagc acgagcacta ccgagatgat ccagaaacgc
     2161 acgatcacgc gcggttcggg ccagcccttc aattcgaagt gatggtggat cggcgccatg
     2221 cggaacagac gcttgccggt gagcttgaaa ctcgccacct gcaagatgac cgatgcggtt
     2281 tccatcacga acacaccgcc catgatcagc agcacgattt cctgacgcac gatcaccgcc
     2341 accgtgccga gcgcggcgcc gaccgcgagc gcgccgacat cgcccatgaa tacctgcgcg
     2401 ggataggcgt tgaaccagag gaagcccagg cccgcgccgg cgagtgcgcc gcagatcacc
     2461 gcgagctcgc cggcgccgac gatgaacgga aattgcagat aacgcgcgaa gacggcgttc
     2521 ccggaaacgt aggcgaatac gcccagcgcc ccggcgacca tgacggtcgg catgatggcg
     2581 aggccgtcga ggccgtcggt gagattgacg gcattgctcg tgccgacgat cacgaagtac
     2641 gtgagcgcga tgaatcccca cagcccgagc ggataggaga tcgtcttgaa gaagggcacg
     2701 atgaggtcgg cctgcggcgg caggctcacg ctgaatccgc tgcgcaccca ctccatgaag
     2761 aggtgcagga actgcgaatt cgacggggcc gacacgctga acgcgagata gaccgccgcg
     2821 acgaccccga tcaccgactg ccagaagaac ttcgaccgcg caggcagtcc cttcgggttg
     2881 cgatacacca ccttgcggta atcgtcgacc cagccgatcg ccccgaaccc gagggtgacg
     2941 agcaggacca cccagacgaa acggttggcg aggtcacccc aaagcagggt ggtcacggag
     3001 accgacacca ggatgagggc gccgcccatg gtgggggtgc cggccttggt gagatgggtc
     3061 tgcggaccat cgtcgcggac cgcctggccg atcttcatgc gcgtgagcca ggcaatcgtg
     3121 cgcggaccga cgatgaagga aat
//
LOCUS       DASRVO010000126         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_211032, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000126 DASRVO010000000
VERSION     DASRVO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211032"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFF05_01450"
     CDS             <1..426
                     /locus_tag="VFF05_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50501.1"
                     /translation="PPAIALGGYMSGNWYDASQSGHGFELEFTGQPSGTGNLNTFVAY
                     WYVYTPDGSGQNWIFAYGPYDTTTNTVTSPAFLLGGTKFPPLFDPNAVTQTAWGTLTF
                     TFTDCNNGTANWTSTIAGYGSGSLPITRITNVAGTTCPQ"
     gene            complement(431..1675)
                     /gene="ispG"
                     /locus_tag="VFF05_01455"
                     /gene_synonym="gcpE"
     CDS             complement(431..1675)
                     /gene="ispG"
                     /locus_tag="VFF05_01455"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZW50502.1"
                     /translation="MNSEHQNASVRPRRHSVGVNVGPVQVGGDAPIVVQSMTNTDTAD
                     VAGTAKQVAELARAGSELVRITVNTPEAAAAVPRIRERLDMMGVGAPLIGDFHYNGHQ
                     LLAAEPACAQALAKYRINPGNVGFGKKRDTQFSAIVDFALKYEKPVRIGANWGSLDQS
                     MLAALMDENHSRAVPWDAARVAREALIRSALDSAVRAQELGLTSDRILLSCKVSGVQE
                     LIAVYRDLATRGDYALHLGLTEAGMGSKGIVASTAALAVLLQEGIGDTIRISLTPEPG
                     ESRTREVIVAQELLQTMGLRAFTPLVTACPGCGRTTSTLFQQIAKTVQDHVRVKMPEW
                     RVKYDGVENLTLAVMGCVVNGPGESRHADIGISLPGTGEAPAAPVFIDGQKAMTLRGE
                     NIAQEFVGVVEDYVARKFVARH"
     gene            complement(1728..>2035)
                     /locus_tag="VFF05_01460"
     CDS             complement(1728..>2035)
                     /locus_tag="VFF05_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50503.1"
                     /translation="RAAMRALALEDLHAVGATPVVIVPALFDRDATEIELTRPGDPFS
                     WPVEPSIETLRDLRASDNGGGYFRIDMRTLDGDAATNAPRAANDDPGNTVSVRGEGA"
BASE COUNT          354 a          690 c          617 g          374 t
ORIGIN      
        1 ccgcctgcga tcgcgctggg cgggtacatg agcggcaact ggtacgacgc cagccagagc
       61 ggccatggct tcgaattgga atttacgggc cagcccagcg gcaccggcaa cctgaacacc
      121 ttcgtcgctt actggtacgt ctacacgccg gacggtagcg gccagaactg gatcttcgcc
      181 tacggtcctt acgatacgac caccaatacc gtgacttcgc ccgcttttct gctcggcgga
      241 accaaattcc cgccgctgtt cgatcccaac gcggttacgc aaaccgcctg gggcacgctg
      301 acgtttacct tcaccgactg caacaacggc acggcgaatt ggacttcgac catcgccggc
      361 tacggcagtg gcagtctgcc gatcacgcgc attaccaacg tggcgggtac gacatgtccg
      421 caatgagggt tcaatgtcgc gcgacaaact tgcgcgcgac gtagtcttcg acgacgccga
      481 cgaattcctg cgcgatgttc tcgccgcgca gcgtcatcgc cttctggccg tcgatgaaaa
      541 ccggcgccgc cggcgcctcg ccggttccgg gcaacgagat gccgatgtcg gcatgacgcg
      601 attcgcccgg cccgtttacc acgcaaccca tcaccgccaa cgtgagattt tcgacgccgt
      661 cgtatttgac gcgccattcg ggcatcttga cgcggacgtg atcctgcacg gtcttggcaa
      721 tttgctggaa caacgtgctc gtcgtgcgtc cgcaacccgg acaggccgtc accagcgggg
      781 tgaacgcgcg cagtcccatg gtctgcaaca actcctgggc cacgatcacc tcgcgcgtgc
      841 gcgactcgcc cggctcgggc gtcagcgaga tgcgaatcgt gtcgccgatg ccttcctgca
      901 gcagtaccgc caacgccgca gtcgaggcga caatgccctt gctgcccatg cccgcctcgg
      961 tcaatcccag gtgcagcgcg tagtcgccac gggtcgccag atcgcggtag acggcgatca
     1021 attcctgaac cccggaaacc ttgcaagaca ataagatacg atctgaagtt aatcccagtt
     1081 cttgcgcccg caccgctgaa tccagcgccg accggatcag tgcttcgcgc gcaacccgtg
     1141 ccgcgtccca gggcacggct cggctgtggt tttcgtccat caacgctgcc agcatggact
     1201 ggtcgagcga gccccagttg gcgccaatgc gcaccggttt ttcgtatttc aacgcgaaat
     1261 cgacaatggc cgagaattgc gtatcgcgct tcttgccgaa accgacgttg ccgggattga
     1321 tccggtattt ggccagcgcc tgcgcgcaag ccggttcggc ggcaagcagc tgatggccgt
     1381 tgtaatggaa gtcgccgatc agcggcgcgc cgacgcccat catgtccagg cgctcgcgga
     1441 tgcgcggcac ggcggcggcg gcttccggcg tgttgacggt gatgcgcacg agttcggatc
     1501 cggcgcgcgc cagttcggca acctgtttcg cggtgccggc gacatcggcg gtatcggtat
     1561 tcgtcatcga ctgcacgacg atgggcgcgt cgcctccgac ttgcacgggg ccgacgttca
     1621 cgcccacgct gtgccgacgc ggccgcacgc tcgcattctg gtgttcggaa ttcattgccg
     1681 tccggaattt gctgacgcga gcagttgacc cgttcgccgt gcggtcgtca agcgccctcg
     1741 ccgcgcacgc tgacggtgtt tccgggatcg tcgttcgcgg cccgcggcgc attcgtcgcc
     1801 gcatcgccat ccagcgtgcg catatcgatg cggaaatatc cgccgccgtt gtcgctggcg
     1861 cgcaagtcgc gcaaggtttc gatggacggc tccaccggcc aactgaacgg atcgcccggc
     1921 cgggtcaact cgatctcggt ggcatcgcga tcgaacaaag ccggcacgat gacgaccggc
     1981 gtcgcgccga ccgcatgcag gtcttccagt gccaacgcgc gcatcgccgc ccgcg
//
LOCUS       DASRVO010000127         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_107252, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000127 DASRVO010000000
VERSION     DASRVO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107252"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1020
                     /locus_tag="VFF05_01465"
     CDS             <1..>1020
                     /locus_tag="VFF05_01465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW50504.1"
                     /translation="ARLLVLHACGGGTLRYAELVANVLRRQGIRPIFAWGSDGRELCI
                     SSSDPSRADSRHSLPEGIDDVAKLLHRLRVTRVDVLHSLGVEESLPKLLRALAVPYDV
                     TLLDYHLFAHEPHLMRGDGLTPARNQGDELSALRPLLRSGVHPLLQGASRIVACSRDL
                     TARLLRLAPDLRIRAVRPPEPLRAEAFRVTPPVALERGEALRVLVLGHLVRAKGSQEL
                     LAVATQAKQRDLCLEFHHLGVQIDKMAPREQACAALRLHGFFRREDLTTIVNRIRPHL
                     AWFPARAPETWGFTLSEAMSMGLPILARGVGAYPERLAGRAFTWVVRAEEQSPEFWLE
                     RMIALR"
BASE COUNT          148 a          337 c          343 g          192 t
ORIGIN      
        1 gcgcgtttgc tcgtgctgca cgcatgcggc ggcggcacgc tgcgctacgc ggaactggtc
       61 gcgaatgtct tgcggcgcca aggcatccgc ccgatattcg cctggggcag cgacggccgc
      121 gagttgtgca tttccagttc cgatccttca cgagcggatt cacgtcactc cctgccggag
      181 gggatcgatg acgtggccaa actgctgcac cggctgcggg tgacgcgcgt cgacgtgttg
      241 cactcgctcg gcgtggaaga gtccctgccg aagctgctcc gcgcgctcgc ggtgccctac
      301 gacgtgacct tgctggacta ccacctgttt gcgcacgaac cgcatctgat gcgcggcgac
      361 ggactcacac cggcgagaaa ccagggcgac gaattgtccg cgctccggcc cttgttgcgc
      421 agcggcgtgc atcccttgct gcaaggggcc agtcgtattg tggcctgcag ccgcgactta
      481 accgcccgcc tgctccggct ggcaccggac ttgcgcattc gcgcggtacg gccgcccgag
      541 ccgctgcgcg cagaagcttt ccgggtcacg ccaccggttg cgctcgagcg cggcgaggcc
      601 ttgcgcgtgc tggtgctggg acatctcgtg cgcgccaaag gcagccagga attgctcgcc
      661 gtggcgacgc aggccaagca gcgcgacctt tgcctcgaat tccaccatct gggtgtccag
      721 atcgataaga tggccccgcg agagcaggca tgcgccgcac tgcggctgca cggattcttc
      781 cgtcgcgagg atctgaccac gatcgtcaac cgtattcgtc cccatttggc gtggtttccg
      841 gcgcgtgcgc ccgagacttg gggcttcacg ttgtccgaag cgatgtcgat ggggttgccg
      901 atcctggcac gcggcgttgg cgcctatccg gagcgactgg cggggcgcgc cttcacctgg
      961 gttgttcgcg ccgaggagca atcgccggaa ttctggctcg agcggatgat cgcgttgcgc
//
LOCUS       DASRVO010000128         1745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_159255, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000128 DASRVO010000000
VERSION     DASRVO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159255"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFF05_01470"
     CDS             complement(<1..190)
                     /locus_tag="VFF05_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50505.1"
                     /translation="MTQRVLRHKARALFGNFSVAPAALYPLYESSISLRGETNFSADA
                     AAVSSRTQSRVFCANGGEG"
     gene            complement(187..1701)
                     /gene="xylB"
                     /locus_tag="VFF05_01475"
     CDS             complement(187..1701)
                     /gene="xylB"
                     /locus_tag="VFF05_01475"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HZW50506.1"
                     /translation="MAKRVLGIDVGTGGTRAVLLDETGKVVASATSEHAPISAPQIGW
                     AEQDPRDWWRATCAAIQECLQLAHASPEEIAGVGLTGQMHGMVLLDAAGEVLRPAIIW
                     CDQRTDPQCRAITEKVGAHRLIELTANPALTNFTLTKIWWVQQHEPHVWSRVRTVLLP
                     KDYARFRMTGARACDVADASGTLLFDVAHRRWSAPMAEISGIQPEILPQIFESPEISG
                     RISEEGARATGLRAGTPVVAGAGDQAAGAVGMGIVRPGAVSATIGTSGVVFAATAAPT
                     MDPQGRIHTFCHAVPGRWHVMGVTQSAGFSLSWFRDSFCAANDGNRVSYDQLTAEAAK
                     TKPGADGLLWLPYLMGERTPHLDPDARGALVGLTAQHTRGHVVRAILEGVAFSLRDTF
                     TIFRELKLPIESIRLGGGGARGPLWRQIQADVYGMPVEILGAEEGAAYGAGLLAGVGV
                     GVWSSIEKACDVAVKVAQVVQPDSARVEIMNRRYDEYRALYPALKESFAHAKSA"
BASE COUNT          301 a          551 c          601 g          292 t
ORIGIN      
        1 atccctcacc accattcgcg cagaaaacgc gcgactgggt tctggatgac accgcggcgg
       61 cgtccgcgga gaaattcgtc tcgcctctca aggagatcga cgattcgtac agaggataca
      121 gagcggcggg ggccacagag aaattcccga agagggcacg cgctttgtgg cgcagcacgc
      181 gttgggtcac gcggacttgg cgtgcgcgaa gctctccttc agagcggggt agagggcgcg
      241 gtattcgtcg tagcggcggt tcatgatttc gacgcgggcg gaatcgggct ggacgacctg
      301 cgcgactttc accgcgacgt cgcatgcctt ctcgattgaa gaccacacgc cgacgccgac
      361 cccggcgagc agacccgcgc cataggctgc accttcttcg gctccgagaa tttccaccgg
      421 catgccgtag acgtccgcct gaatctgtcg ccagagcggg ccgcgcgcgc cgccgcctcc
      481 gaggcgaatg gattcgatgg ggagcttcag ttcgcggaaa atggtgaagg tgtcgcgcag
      541 gctgaacgcc acgccctcca ggatcgcgcg cacgacgtga ccgcgcgtgt gctgcgccgt
      601 caggccgacc agcgcgccgc gcgcgtcggg atcgagatgc ggcgtgcgtt cgcccatcag
      661 gtagggaagc cagagcaggc cgtccgcgcc gggctttgtc ttggcggcct cagcagtcaa
      721 ctgatcgtac gagacgcgat tgccgtcgtt ggcggcgcag aacgaatcgc ggaaccagct
      781 caacgaaaaa ccggcggact gcgtcacgcc catgacgtgc cagcgtccag ggacggcgtg
      841 gcaaaacgtg tgaatgcggc cttgcggatc catcgtgggc gcagcggtcg cagcgaaaac
      901 gacgccggag gtgccgatcg tcgcgctgac cgcgcctggg cgcacgatgc ccattcccac
      961 agcgccggcg gcttggtcgc ccgcgccagc aaccacgggc gtgccggcgc gcaagccggt
     1021 ggcccgcgcc ccttcctcgg agatccggcc gctgatttca ggcgactcga aaatttgcgg
     1081 cagaatttcg ggctgaatgc ccgagatttc ggccatgggc gcggaccagc ggcgatgagc
     1141 aacatcgaag agaagcgtgc cggaggcgtc cgctacgtcg caggcgcgcg cgccggtcat
     1201 gcggaatcgc gcgtagtctt tcggcaggag cacggtacgt acgcgggacc agacgtgcgg
     1261 ttcgtgctgc tggacccacc agattttcgt cagcgtgaag ttggtgaggg cgggattggc
     1321 ggtcaattcg atcaaccggt gcgcgccgac tttttcggtg atggcgcggc attgcgggtc
     1381 ggtgcgctgg tcgcaccaga tgatcgcggg gcgaagaact tcgcccgccg catcgagcag
     1441 gaccatgccg tgcatctggc cggtcaggcc aacgccggcg atttcttccg gcgatgcgtg
     1501 cgcgagctgc aggcactcct ggatggcggc gcaggtggcg cgccaccagt cgcgcgggtc
     1561 ctgttcggcc cagccgatct gcggggcgga gatgggcgcg tgctcgctgg tcgcggaggc
     1621 cactactttt ccggtttcat cgagcagcac agcgcgcgtg ccgccggtac ccacatcaat
     1681 tccgagaact cgcttcgcca ttgaccatca ccatacgttc tattgtggcc ggggctgtcg
     1741 aatat
//
LOCUS       DASRVO010000129         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_107361, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000129 DASRVO010000000
VERSION     DASRVO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107361"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFF05_01480"
     CDS             <1..556
                     /locus_tag="VFF05_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HZW50507.1"
                     /translation="PSARGNGEPLTGRFTPAPGARFDAVAIADPQTRLVLATDYGYGF
                     VTRFEALLANKKAGKQLVNMDEDAHVLAPAYVNDPARDRIVVATNAGHLLMFSVAELP
                     ELDNGGKGNKLIEIPKAKLVGGERAAGIAVVAEGKGEVTLWAGQRKLILKWADLVEYN
                     GNRAQRGGNLPRGFTRVDRIETTG"
     gene            complement(578..>1673)
                     /locus_tag="VFF05_01485"
     CDS             complement(578..>1673)
                     /locus_tag="VFF05_01485"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZW50508.1"
                     /translation="RDARSPVAHATTLSGVISGGNSQHVVVMNGTAGTSITATTVLDG
                     FTLTGGNNTNGKGGGALWCQGNGAGHACNPTLANLVFSGNHAGNGGAMALDGYSGGAS
                     NPTLTNIAFSNNSASTFGGAMYCNAQLTGGNCSPTLTNVTFSTNSSGTYGGAMFNDGS
                     GSGNSSPILTNVTFSGNSAASSGGAIFNNGGGGTSSPTLTNVTFNGNSASYGGAMYNS
                     GGTNGNSSPTLNNVILWGDTATTSGAELYHSASGSGSAVSFIYYGIIEGDCQINMSCS
                     NTTASDPQLGTLGDNGGFTLTLMPGSAGSAVNTVPCYLTPLTDQRGAARPDPASAALP
                     KRCDMGAVEAGSLAGDVIFIGTFGQAPRDE"
BASE COUNT          285 a          542 c          536 g          310 t
ORIGIN      
        1 gccatcggcg cgcggcaacg gcgagccgtt gaccgggcgc ttcacgcccg caccgggcgc
       61 gcgtttcgac gccgtcgcga tcgccgatcc gcagacccgg ctcgtgctcg ccacggatta
      121 cggctacggc ttcgtaaccc ggttcgaagc gctgctcgcg aacaagaagg ccggcaagca
      181 gctcgtcaac atggacgagg acgcccatgt gctcgcaccg gcgtacgtca acgatcccgc
      241 gcgcgaccgc atcgtcgtcg ccacgaatgc cggacacctg ctcatgtttt cggttgccga
      301 actgccggaa ctcgacaacg gcggcaaggg caacaagctg atcgaaatcc cgaaggccaa
      361 gctcgtcggc ggcgagcgcg ccgccggcat tgccgtggtc gccgaaggca agggtgaagt
      421 cacgctctgg gccggtcagc gcaagctgat cctgaaatgg gcggacctgg tcgaatacaa
      481 cggcaaccgt gcgcagcgcg gcggcaacct gccgcgcggc ttcacgcgcg tggatcgcat
      541 cgagacgacc ggctgaggct ggtgcagccc cgagcggtca ttcgtcccgc ggcgcctgcc
      601 cgaaagtgcc gatgaagatc acatcccctg caagcgaccc ggcttcgacg gcgcccatgt
      661 cgcaacgctt tggcagggcg gcgcttgccg gatccggacg cgccgcgccc cgctgatcgg
      721 tcaacggcgt gagatagcac ggtaccgtgt tgactgccga gccggccgat cccggcatca
      781 atgttaacgt gaatccgccg ttgtcgccca gcgtcccaag ctgcggatcg ctcgcggttg
      841 tgtttgaaca actcatgttg atctgacaat cgccttcgat gatgccgtag tagatgaacg
      901 ataccgcgct gccgctgccg ctggcactgt ggtacagctc ggcaccgctg gttgtcgcgg
      961 tgtcgcccca caggatcacg ttgttcagcg tcgggctact gttgccattg gtaccgcccg
     1021 agttgtacat cgcgccgccg taactggcgc tattgccgtt gaaggtgacg ttggtcaacg
     1081 tcgggctgct ggtaccgccg ccgccattgt tgaaaatcgc gccgccggaa ctggcggcac
     1141 tgttaccgct gaaggtgacg ttggtcagta tcgggctgct gttgccgctg ccggaaccgt
     1201 cgttgaacat cgcgccacca taggtgccgc tgctgttggt gctgaaagtg acgttggtca
     1261 gagtcggact gcagttgccg ccggtaagct gggcattgca atacatcgcg ccaccgaaag
     1321 tgctggcgct gttgttactg aaggcgatgt tcgtcagcgt cgggttgctt gcgccacccg
     1381 aataaccatc aagcgccatg gcaccaccgt tgcccgcatg gttgccgctg aacaccagat
     1441 tggcgagcgt cgggttgcat gcatggcctg caccgttgcc ttggcaccac aacgcaccgc
     1501 cccccttgcc gttggtgttg ttgccgccgg tcaaggtaaa gccgtccagc acggtggttg
     1561 ccgtgatcga cgtgcccgcg gtgccgttca tcacgaccac atgctgggag ttgccgccgc
     1621 tgattacgcc ggacagtgtc gttgcatgcg ccaccggact gcgcgcatcg cgg
//
LOCUS       DASRVO010000130         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_263112, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000130 DASRVO010000000
VERSION     DASRVO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263112"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VFF05_01490"
     CDS             complement(<1..617)
                     /locus_tag="VFF05_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017002098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZW50509.1"
                     /translation="MLLTLVLALILLSTLLFLGYGFFAWTGAAAIWLIGWRICGIASP
                     LLFEGTVVVMIVLAAIFGIPFLRRQIVSRFAMKLMAPVLPRLGDTERIALEAGTVWWD
                     AELFGGRPDWEKLLAFKPQLLSPEEQAFLDGPVNELCARLDDWQIQQQRDLPASVWAF
                     MKNNGFFGMGIPKDYGGHAFSAIMHSRVVTRLSTRSVAAAVTVMVP"
     gene            complement(680..1021)
                     /locus_tag="VFF05_01495"
     CDS             complement(680..1021)
                     /locus_tag="VFF05_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50510.1"
                     /translation="MRIGIGVMLGIGLVVAGCGGGRNDTAAAACSSEIGKRMNGRNFT
                     LDLKDLAAHAKQESADTLLFTSTVVLDKGLPSEDKQTYECRVRFDASGAPSVLYLQFN
                     WNTSDLKQQPQ"
     gene            complement(1137..1772)
                     /locus_tag="VFF05_01500"
     CDS             complement(1137..1772)
                     /locus_tag="VFF05_01500"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HZW50511.1"
                     /translation="MAAGTANPAPRFLALGDSYTIGEGVTANKIWPHQLVAALRAQGV
                     AIADPEIVARTGWTTDELSAAMDAHAFHPPYALVTLLIGVNNQYRGRDVDNYRAEFRK
                     LLERAIALAGGDARHVVVVSIPDWGVTRFGAGSGRDLARIALEIDAFNAVAAQISTTL
                     HAYYVDVTAISRDHGGAPDMLVGDGLHPSAAMYARWTAAIAIQAQAALHGR"
BASE COUNT          327 a          574 c          606 g          293 t
ORIGIN      
        1 ggcaccatga ccgtgacggc ggcggcgacc gagcgcgtcg acagccgggt gacgacacgc
       61 gaatgcatga tcgccgagaa cgcatggccg ccgtagtcct tcggaatgcc catgccgaag
      121 aagccgttgt tcttcatgaa cgcccagacg ctggccggca ggtcgcgctg ctgctggatc
      181 tgccagtcgt cgaggcgcgc gcacagctcg ttgaccggcc cgtcgaggaa cgcctgctcc
      241 tcggggctca gcagctgcgg cttgaacgcc agcagctttt cccagtccgg gcggccgccg
      301 aacagttcgg cgtcccacca gaccgtgccg gcttccagcg cgatgcgctc ggtgtcaccg
      361 agccgcggca gcaccggcgc catcagcttc atggcgaagc gcgaaacgat ctgccggcgc
      421 aaaaacggga tgccgaagat cgccgccagc acgatcatca cgacgaccgt gccttcgaac
      481 agcagcggcg aggcgatgcc gcagatgcgc cagccgatca gccagatcgc ggcggcgccc
      541 gtccaggcaa agaatccgta gccgaggaac aacagcgtgc tgagcagaat cagcgcgagg
      601 acaagcgtga ggagcatggg cgcaccgcgt ttgagccggt cggaccccta tcatccgccg
      661 tggacgcgaa aaagcaaaac tattgcggct gctgcttcaa atccgatgta ttccagttga
      721 attgcaggta cagcacgctt ggcgcgccgg aggcatcgaa gcgcacgcgg cattcgtagg
      781 tttgcttgtc ttcgctcggc aagcctttgt cgagcacgac ggtggacgtg aacagcagcg
      841 tgtcggcgga ttcctgcttg gcgtgcgcgg ccaggtcttt cagatcgagc gtgaaattgc
      901 gcccgttcat gcgcttgccg atttcgctgc tgcacgccgc cgccgcggtg tcgttgcgcc
      961 cgccgccgca gccggcgacg acgagaccga tgccgagcat tacgccaatg ccgatgcgca
     1021 tatcgccctc ccgtgtttgc gccgtgcgga aagctccgcc ggtcgcatgg cttccggcgc
     1081 atgttacatc tcagggacgc cggtgcaagc catggcggca gcggatttga tgcaaatcaa
     1141 cgcccgtgca gtgcggcctg cgcctgaatg gcgattgcgg cggtccagcg cgcgtacatg
     1201 gcggcggatg gatgcaggcc atcgccaacc agcatgtcag gagcaccgcc atggtcgcgg
     1261 gagatcgccg tcacgtcgac gtagtaggcg tgcagggtcg tggaaatctg cgctgccacg
     1321 gcattgaagg catcgatttc gagtgcgatc cgggcaagat cgcgcccgct tccggcgccg
     1381 aagcgggtga cgccccaatc cggaatggag acgacgacga cgtgccgcgc atcgccaccg
     1441 gcgagtgcaa tcgcacgttc cagcagcttg cggaattcgg cgcggtagtt gtcgacatcg
     1501 cgaccgcggt actggttgtt gaccccgatc agcagcgtga ccagcgcgta gggcggatgg
     1561 aacgcgtgcg catccatcgc ggcggacaac tcgtccgtgg tccagccggt gcgcgcgacg
     1621 atttcagggt ctgcgatcgc gacgccttgt gcacgcagcg cggcgacgag ctgatgcggc
     1681 cagattttgt tcgccgtgac gccttcgcca atggtgtagg aatcgcccag ggcgaggaag
     1741 cgtggggctg gattcgcggt tcccgcggcc agcgcgacgc cagcggcgag gctaagccac
//
LOCUS       DASRVO010000131         1132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_315270, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000131 DASRVO010000000
VERSION     DASRVO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315270"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1132
                     /locus_tag="VFF05_01505"
     CDS             <1..>1132
                     /locus_tag="VFF05_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZW50512.1"
                     /translation="TVLVLVGQVLELRARDATSGAIKALLNLAPKTARRVKADGSDEE
                     VSLDAIQIGDSLRVRPGDKVPVDGTVLEGRSALDESMVTGESMPVAKENGSRVIGGTL
                     NTTGSFVMRADKIGRDTLLSQIVQMVASAQRSRAPIQRLADRVAGWFVPTVIAVALIA
                     FAAWATFGPEPRFAFGLVAAVSVLIIACPCALGLATPMSIMVGVGRGAQEGVLIKNAE
                     ALERMERVDTLVVDKTGTLTEGKPKVVAIVAAAGFEESEILRLAASVERASEHPLADA
                     IVRSARARNLDLSKVEEFDSPTGKGATGKVDGKTVLLGNSNFLTSLGIETRSLSKQAE
                     NLRGEGATVVNIAIDERLAGLFAIADPVKQSTPDALNALAAEG"
BASE COUNT          200 a          357 c          393 g          182 t
ORIGIN      
        1 acggtgctgg tgctggtcgg ccaggtattg gaattgcgcg cgcgcgatgc cacctccggc
       61 gcgatcaagg ctctgcttaa cctcgcgccg aaaaccgcgc gccgcgtcaa ggccgacggc
      121 agcgacgagg aagtctccct cgacgcgatc cagatcggcg atagtttgcg cgtgcgtccc
      181 ggcgacaagg tgccggtcga cggcaccgtg ctggaaggcc gttccgcgct cgatgaatcc
      241 atggtcaccg gggaatccat gcccgtcgcc aaggaaaacg gttcgcgcgt gatcggcggc
      301 acgctcaata ccaccggcag cttcgtcatg cgcgccgaca agatcggccg cgacacgctg
      361 ctctcgcaga tcgtgcagat ggtggcgagt gcgcagcgca gccgcgcgcc gatccagcgg
      421 ctggccgacc gcgtcgccgg ctggttcgtg ccgacggtga tcgcggtggc gctcatcgcc
      481 ttcgccgcct gggcgacctt cggcccggag ccgcgctttg ccttcggcct ggtggccgcg
      541 gtcagcgtgc tgatcatcgc ttgcccctgc gcgctcgggc tcgccacgcc gatgtcgatc
      601 atggtcggcg tcggccgcgg cgcccaggaa ggcgtgctga tcaagaacgc cgaagcgctg
      661 gagcggatgg aaagagtcga tactttggtg gtcgacaaga cggggacgct gaccgaaggc
      721 aagccgaaag tcgtcgccat cgttgccgcg gcgggatttg aggagagcga gattctgcgg
      781 ctcgccgcca gcgtggagcg cgccagcgaa catccgctcg ccgatgcgat cgtgcggagc
      841 gccagggcac gcaatctcga tttgagcaag gtggaggagt tcgactcgcc gaccggcaag
      901 ggcgcgaccg gcaaggtcga tggcaagacc gttctgttgg gcaactcgaa cttcctgaca
      961 tctctcggta tcgaaacccg gtcgttgagc aagcaggcag aaaatttgcg cggagaaggc
     1021 gccaccgtcg tcaacatcgc gatcgatgaa aggctcgccg ggctgtttgc gatcgccgat
     1081 ccggtcaaac aatcgacgcc ggatgcgctg aacgcgctgg cggcggaagg ca
//
LOCUS       DASRVO010000132         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_366870, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000132 DASRVO010000000
VERSION     DASRVO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366870"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFF05_01510"
     CDS             <1..480
                     /locus_tag="VFF05_01510"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016945080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZW50513.1"
                     /translation="LVPIGLGLGRIGNFINGELWGKLTGGDWGVIYPRALESLGKSTA
                     ELHRMFLDGQLNHEARHPSELYEFALEGVVMFVVLVLFSRKPRPRYAVSGLFALMYGV
                     FRFVVEFLRLPDPQLGYLAFGWLTMGQLQSIPLIVVGVLLLWMSRRAPTLPLAKAAA"
     gene            480..>1181
                     /locus_tag="VFF05_01515"
     CDS             480..>1181
                     /locus_tag="VFF05_01515"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004426031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZW50514.1"
                     /translation="MRQYLDLLRDVLDNGAEKSDRTGTGTRSLFGRQIRFDLGDGFPL
                     VTTKKLHTKSIVHELLWFLRGETNIAYLKENGVSIWDEWADADGNLGPVYGKQWRSWQ
                     GADGKTIDQIAWLIDEIRRNPDSRRLVVNAWNVAELPKMALAPCHCLFQFHVANGRLS
                     CQLYQRSADVFLGVPFNIASYALLTHLVAQVCDLAPGDFVHSFGDVHLYNNHVEQART
                     QLAREPLPLPKLKLNP"
BASE COUNT          197 a          368 c          372 g          244 t
ORIGIN      
        1 ctggtgccga tcggccttgg cctcggtcga atcggcaact tcatcaacgg cgaactgtgg
       61 ggcaagctca ccggtggcga ctggggcgtg atctatccgc gcgcgctgga atcgctgggc
      121 aagtcgacgg cggagttgca ccggatgttc ctcgacgggc agctcaacca cgaggcacgc
      181 catccgtcgg agttgtacga atttgccctc gaaggcgtgg tcatgttcgt cgtgctcgtg
      241 ctgttttcgc gcaagccgcg gccgcgttac gccgtgtcgg gcctgttcgc attgatgtat
      301 ggcgtgttcc gcttcgtcgt ggaattcctg cgcctgcccg atccgcagct gggttatctc
      361 gcgttcggtt ggctgaccat gggacaactg cagtcgattc cgctgatcgt cgtcggcgtc
      421 ttgctgttgt ggatgtcgcg gcgcgcgccg acgttgccgc tcgccaaggc cgccgcctga
      481 tgcgccagta tctcgacctg ctgcgcgacg tgctggacaa cggcgcggaa aaatccgatc
      541 gcaccggtac cggcacgcgc tcgctgttcg gccggcaaat tcgtttcgat ctcggcgacg
      601 gctttccgct ggtcacgacc aagaagctgc ataccaagtc catcgtgcat gaattgctgt
      661 ggttcctgcg cggcgaaacg aacatcgcct acctgaagga aaatggcgtc tcgatctggg
      721 acgaatgggc cgacgcggac ggcaacctag gtccggttta cggcaagcaa tggcgatctt
      781 ggcaaggcgc ggatggcaag acgatcgacc agatcgcctg gctgatcgac gaaatccggc
      841 gcaatcccga ttcgcgccgt ctcgtggtca atgcgtggaa cgtggccgaa ttgccgaaga
      901 tggcgctcgc gccctgccac tgcctgttcc agttccatgt cgcgaacgga cgcttgtcgt
      961 gccagttgta ccagcgctcg gcggatgtct tcctcggcgt gccgttcaat atcgccagct
     1021 acgccctgct gacgcacctg gtcgcgcagg tctgcgatct cgcgcccggc gatttcgtgc
     1081 acagcttcgg cgacgtgcat ttgtacaaca accacgtcga gcaggcgcgc acgcaactcg
     1141 cgcgcgagcc attgccgctg ccgaaactga agttgaaccc g
//
LOCUS       DASRVO010000133         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_81689, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000133 DASRVO010000000
VERSION     DASRVO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81689"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VFF05_01520"
     CDS             complement(<1..918)
                     /locus_tag="VFF05_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZW50515.1"
                     /translation="MRAAEVLPRIAVDAVALIDQMATGIAVLDGDLRFIHANAAFVEL
                     TGLARWRGCPLEVLGETADALTALIECAQTVQAPSTLRGLEISARSASVRVDITAAEC
                     AGGVLLEMHALNPHDASPAQISQSLRGLAHEVKNPLAGLRGAAQLLQRRAADADQRRL
                     AELIIAEADRLGALTDRLLHPSGKPHLATVNLHEVAERARALIAIEAAPEIKLDRDYD
                     PSLPVFRGDADRLQQVLLNLLRNALQAGATQIRLRTRAESGAIVNGQTLRLALRLDVI
                     DNGSGVPDSLRETLFAPLVSGRADGTGLGL"
     gene            1008..2327
                     /locus_tag="VFF05_01525"
     CDS             1008..2327
                     /locus_tag="VFF05_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50516.1"
                     /translation="MSLRSLFVDFNSYFASVEQQADPALRGRPVGVAPVMAETTCCIA
                     ASVEAKTFGVRTGTLVREARKLCPQIAIVPAQPSLYVEYHHRLKDAIETCIPIDYVGS
                     IDEVACELIGRERVRANAVAIAQRIKTAVRGVGDWLRCSVGIAPNHFLSKTATDMQKP
                     DGLVVLEQSDLPHALHRLELSDLCGIGPNMEQRLHTGGIRTVAHLCALDAPAMREVWG
                     GIEGERFFEALRGQWQTHRESARASLGHSHVLGPQWRTPAGARSVLKKLLVKAAMRLR
                     HEGFVAAALSVRVRHLGADAWHGESRFESTDDSREFLRQLAHALEQRDRHTLRLPLRG
                     GKPIPLAVGVTLHHLQRRGESTGSLFANEAKNRAVNGVLDRINQRYGGNTVYFGGMQS
                     ALEAAPMRIPFSSIPDPHLERDAGHNDLWLKRMREFKRQAEVAHQRG"
     gene            complement(2367..>3136)
                     /gene="glnA"
                     /locus_tag="VFF05_01530"
     CDS             complement(2367..>3136)
                     /gene="glnA"
                     /locus_tag="VFF05_01530"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972068.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HZW50517.1"
                     /translation="ATAGQCEIGTKFSTLVHKGDELLTLKYVIKNVAHAHGKTVTFMP
                     KPVVGDNGSGMHVHQSLAKGGTNLFAGDLYGGLSQTALYYIGGIFKHARAINAFTNST
                     TNSYKRLVPGFEAPVMLAYSARNRSASCRIPFVHSPKARRIEVRFPDPMNSGYLAFTA
                     LMMAGLDGILNKIDPGAPADKDLYDLPPEEEKNIPQVCSSLDQALEALHKDRAFLKAG
                     GVFSDDFIDAYIETKLAEVTRFRAATHPVEFAMYYSL"
BASE COUNT          554 a         1026 c         1020 g          536 t
ORIGIN      
        1 caggccgagc cccgtgccgt ccgcgcgccc ggacaccagc ggcgcgaaca gcgtttcgcg
       61 caaagagtcc ggcacaccgg aaccgttgtc gatcacgtcc aggcgcaacg caaggcgaag
      121 cgtttgtccg ttgacgatcg cgccgctttc cgcacgcgtg cgcaagcgaa tctgcgtcgc
      181 accggcctgc aaggcattgc gcagcaaatt cagcaacacc tgctgcaggc ggtcggcgtc
      241 gccgcggaag acgggcaggc tcggatcgta atcgcggtcg agtttgattt caggcgcggc
      301 ttcgatggca atcaatgcac gtgcgcgctc ggcgacttcg tgcagattga ccgtggctag
      361 atgcggcttg ccggacggat gcagcagacg atccgtcagc gcgccgagcc gatcggcttc
      421 cgcgatgatg agttcggcca ggcgccgttg gtccgcgtcg gcggcgcggc gttgcagcag
      481 ttgcgccgca ccgcgcaggc cggcgagcgg attcttcacc tcgtgcgcga gtccgcgcag
      541 gctttgcgag atttgcgccg gcgacgcgtc atgcggattc agcgcgtgca tctccagcag
      601 cacgccgcca gcgcactctg cggcggtgat atcgacgcga accgatgcgg aacgcgcgga
      661 gatttccagg ccgcgcagcg tcgacggcgc ctgcacggtc tgcgcgcact cgatcaacgc
      721 ggtcaatgcg tcagcggttt cgcccagcac ttccagcgga cagccgcgcc agcgcgccag
      781 ccccgtcagt tcgacgaagg cggcgttggc gtggatgaaa cgcaaatccc cgtcgagcac
      841 ggcgataccc gtcgccatct ggtcgatcag ggcaacggca tcgacggcaa ttctcggcag
      901 gacttcggcg gcacgcattg caccatttta gtgcagaagt acccatgtcg ccattcctga
      961 gatcggagta ggctcaactc ccggcacgcg tccgccggaa tcgtgccatg agcctgcgca
     1021 gtctcttcgt cgacttcaat tcctatttcg cctcggtcga gcaacaggcc gatccggcgc
     1081 tgcgcgggcg accggtcggc gtcgccccgg tgatggccga aacgacctgc tgcatcgccg
     1141 ccagtgtcga agcgaagacc ttcggcgtgc gcaccggcac cctcgtgcgc gaggcgcgca
     1201 agttgtgccc gcagatcgcg atcgtgccgg cgcagccctc gctgtacgtc gaataccatc
     1261 atcgcttgaa ggacgcgatc gaaacctgca ttccgatcga ctacgtcggc tcaatcgatg
     1321 aagtggcttg cgaactgata gggcgcgaac gcgtgcgcgc aaatgccgtc gccattgcgc
     1381 agcgcatcaa gaccgccgtg cgcggcgtcg gcgactggct gcgctgctcg gtcggcatcg
     1441 cgccgaatca tttcctgtcc aagaccgcca ccgacatgca aaagcccgac ggcctggtcg
     1501 tgctggaaca aagcgatctg ccacacgcac tgcatcggct ggaattgtcc gacctgtgcg
     1561 gcataggccc gaacatggaa cagcgcctgc acacgggcgg catccgcacc gtcgcgcact
     1621 tgtgcgcact ggatgcgccc gcgatgcgcg aagtctgggg cggcatcgaa ggcgagcgct
     1681 tcttcgaggc gctgcgcggg caatggcaga cgcatcgcga atccgcgcgt gccagcctcg
     1741 gccattcgca tgtgctcggt ccgcaatggc gcacgccggc tggcgcgcgt tcggtgctga
     1801 agaaattgct ggtcaaggcg gcgatgcggc tgcgtcacga aggctttgtc gcggcggcgt
     1861 tatcggtgcg cgtgcgccat ctcggcgccg acgcctggca cggcgaaagc cgttttgagt
     1921 cgaccgacga cagccgcgag ttcctgcgcc aactcgcgca cgcactcgaa cagcgcgacc
     1981 ggcacacatt gcggctgccg ctgcgcggcg gcaaaccgat accgcttgcg gtgggcgtga
     2041 ccctgcacca cttgcagcgt cgcggcgaga gcaccggttc attgttcgcg aacgaggcga
     2101 agaaccgcgc ggtgaatggc gtgctcgacc gcatcaacca acgctacggc ggcaacaccg
     2161 tgtatttcgg cggcatgcag agcgcgctcg aggccgcgcc aatgcgcatc ccgttcagtt
     2221 cgattcccga cccgcacctg gaacgcgacg ctgggcacaa cgatttgtgg ctcaagcgca
     2281 tgcgcgagtt caagcgccaa gccgaggttg cgcaccagcg cggctgacgg cgcccgcagt
     2341 acgcggacgc catcgcgaaa cactggtcag aggctgtagt acatcgcaaa ttccaccgga
     2401 tgagtcgccg cccggaagcg cgtgacctcg gcaagcttcg tttcgatgta ggcgtcgatg
     2461 aagtcgtcgc tgaacacgcc gccggccttc aggaatgcgc gatccttgtg caacgcttcc
     2521 agcgcctgat ccagactcga gcacacctgc gggatgttct tttcctcttc cggcggcagg
     2581 tcgtacaagt ccttgtcggc cggcgcgccc ggatcgatct tgttgaggat gccgtccagg
     2641 cccgccatca tcagcgcggt gaaggcgagg taacccgagt tcatcggatc ggggaaacgc
     2701 acttcgatgc gccgcgcctt gggcgaatgc acgaacggaa tgcggcagct cgccgaacgg
     2761 ttgcgcgccg aatacgccag catcaccggc gcctcgaagc ccggcaccag gcgcttgtaa
     2821 ctgttggtcg tggaattggt gaaggcgttg atcgcacgcg cgtgcttgaa gatgccgccg
     2881 atgtagtaca gcgcggtctg cgacaagccg ccgtacaagt cgccggcgaa caaattcgtg
     2941 ccacccttgg ccaggctctg gtgcacgtgc atgccggaac cgttgtcgcc gacgacgggt
     3001 ttgggcatga aggtgacggt cttgccgtgc gcatgcgcga cgttcttgat cacgtacttg
     3061 agcgtgagca gctcatcgcc cttgtgcacc agcgtgctga atttggtgcc gatctcgcac
     3121 tgcccggcgg tggcga
//
LOCUS       DASRVO010000134         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_289644, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000134 DASRVO010000000
VERSION     DASRVO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289644"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFF05_01535"
     CDS             complement(<1..453)
                     /locus_tag="VFF05_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50518.1"
                     /translation="MHTPTLFRSLTGARALRALASLALCALPAPAARAQSTDSVDRPG
                     VPDTSIFAPLRLPTPNEFRLADGEPGPRYWQNRADYDIRATLDTATQTLTGRLTMRYT
                     NDSPVSLDHIWMQVEQDAFTDSSLNAYVFPQQSRFGARGFAGGDRIDSF"
     gene            731..1579
                     /locus_tag="VFF05_01540"
     CDS             731..1579
                     /locus_tag="VFF05_01540"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HZW50519.1"
                     /translation="MPDIGSLHPQVVHFVVALLLVGVLARIVSLIPLGPRWSFTNGMA
                     AALILLGTGAAVVAVQSGLDAHEKVESIPGVRQAVNVHQDYGEDTRDIFLVVSAIEIA
                     MLVLATRKPGLTKGLRALSAVVGIVGAVYLYEAGDHGGDLVYEYAGGVGVRSGDTTDV
                     RRLLVAGLYDNAQVDRRAGQHDAAARLIDQLQSMMPGDTSVRLLRIQSMVRDRKDPRG
                     ALAALDSISVSPDNGRLLFQKTMLEAEALDSAGMRDSARALLTNFKKNVPQAARFVDQ
                     MLQRMH"
     gene            complement(1637..>2484)
                     /locus_tag="VFF05_01545"
     CDS             complement(1637..>2484)
                     /locus_tag="VFF05_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HZW50520.1"
                     /translation="ARSATWVLSIVLAIVFLATGIAKLTGASTFYIQAATMRGFPVWI
                     RIVVGLVEIGGAIALLVPQAAVFGAVTLAILMFPAAATQHMSGQGGFWIPVIVFIMLC
                     VVVWLRSPELIDTLVAYVERPHAVLRGGVMSGVIGATAVAVWFFIVDLIAGHPLFTPD
                     TLGHALFSVTGPVGMHQRAAFIAAYTVFHYAAFIVAGILAASVADMAGEEPSVVLFGV
                     LLFVAFEIWFYAFVAILQHSTPLGSLAWYQVLIGNVIAAVAMVWYLWRARPVLKEQFA
                     HALDT"
BASE COUNT          442 a          802 c          869 g          371 t
ORIGIN      
        1 gaaggaatcg atgcgatcgc cccccgcgaa accacgcgcg ccgaagcgcg attgctgggg
       61 aaacacatag gcgttgagcg aactgtcggt gaaggcatcc tgctcgacct gcatccagat
      121 atggtcgagc gaaaccgggg agtcgttggt gtaccgcatg gtgaggcgcc cggtgagcgt
      181 ctgggtcgcg gtatcgaggg tggcgcggat gtcgtagtcg gcgcggttct gccagtagcg
      241 cggaccgggt tcgccgtcgg cgagccggaa ttcgttaggc gtggggagac gcaacggcgc
      301 aaagatggac gtgtcgggga cgccgggccg gtccaccgag tcggttgact gcgcgcgcgc
      361 ggccggcgcc ggcagtgcgc agagcgcgag gctggcgagg gcgcgcagag cgcgggcgcc
      421 ggtgagcgat cgaaagagtg tcggcgtgtg catgggctcg gtgttccggt ggacgcgacg
      481 gacttcggtc aactcgaagg ttaacggtcg cgccgagatg gtacgaggcc ggccgctgcg
      541 cgcgcgcgac agccggcgct atcggacagc cggcggtatc cgacagccgg cgctatccga
      601 aacggcgcta tccgaaacgg cgctatccga cagcccgcgg gacggttgac gggaggcgaa
      661 tccgcggcgt agcgtgtagc gtcgacgatc agaccgctcg ccgcttctct tcctcccgga
      721 gtccattctc gtgcccgata tcggcagtct ccatccgcag gtggttcact tcgtcgtcgc
      781 gctcctcctc gtgggcgtcc tggcgcgcat cgtgtccctc atcccgttgg gcccgcggtg
      841 gtcgttcacc aacggcatgg ccgccgcgct catcttgtta ggcaccggcg cggccgtggt
      901 cgcggtgcag agcggactcg atgcccacga gaaggtcgaa tcgataccgg gcgtgcggca
      961 ggcggtgaac gtgcaccagg attacggcga ggacacgcgc gacatcttcc tcgtcgtgtc
     1021 cgcgatcgag atcgcgatgc tggtcctggc aacgcggaag cccggcctga ccaagggact
     1081 ccgggcgctc tcggcggtgg ttggcatcgt gggcgccgtc tatctgtacg aggcgggcga
     1141 ccatggcggc gacctcgtgt acgaatatgc cggcggcgtc ggcgtgcgga gcggcgacac
     1201 caccgacgtg cgccgcctgc tcgtggccgg gctgtacgac aatgcccaag tcgatcgccg
     1261 tgcgggccag cacgacgcgg cggcgcggct catcgaccaa ctgcagagca tgatgcccgg
     1321 cgatacgtcg gtgcgcctgc tgcgcatcca gtcgatggtt cgcgaccgga aggatccgcg
     1381 cggggcgctg gcggcgttgg actcgatctc tgtgtcgccc gacaacgggc gactgctgtt
     1441 ccagaagacg atgctcgaag cggaggccct cgactcggcc ggcatgcgcg attcggcgcg
     1501 cgcgctgctc accaacttca agaagaacgt gccgcaggcc gcgcggttcg tcgatcagat
     1561 gctccagcgg atgcactgac cggcgcgccc tcgccctgtt gctttcggcg cgccggccct
     1621 tccgttgggc gagacgtcag gtatccagcg cgtgcgcgaa ctgctccttg agcacgggcc
     1681 gcgcacgcca gaggtaccag accatggcaa ccgcggcgat cacgttgccg atcagcacct
     1741 gataccacgc caaggatccc agcggtgtgg aatgctgcag aatcgcgacg aacgcataga
     1801 accagatctc gaacgcgacg aagagcagca cgccgaacag caccaccgac ggctcctcgc
     1861 cggccatgtc cgctacgctc gcggcgagga ttccggcgac gatgaacgcc gcatagtgga
     1921 acaccgtgta cgcggcgatg aacgcagcgc gctggtgcat cccgaccgga ccggtcacgc
     1981 tgaacagggc gtggccaaga gtatccggcg tgaacaacgg atgccccgcg atgaggtcga
     2041 cgatgaaaaa ccagaccgcc accgcggtcg cgccaatgac gccggacatg acgccaccgc
     2101 gcagcaccgc atgcgggcgc tcgacgtatg cgaccagcgt gtcgatcagt tccgggctgc
     2161 gcagccaaac cacgacgcag agcatgatga agacgatgac gggaatccag aacccgccct
     2221 gcccgctcat gtgctgcgtg gccgcagccg ggaacatcag gatggcgagt gtgacggcgc
     2281 cgaacacagc cgcttgcggc acgagtagcg cgatcgcccc gccgatctcc acgagcccga
     2341 cgacgatgcg aatccacacc gggaatccgc gcatcgtggc ggcctgaatg tagaaggtgg
     2401 aggcccccgt gagcttggcg attcccgtcg ccagaaacac gatcgcgagc acgatgctca
     2461 ggacccacgt ggcggaacgt gccg
//
LOCUS       DASRVO010000135         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_55760, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000135 DASRVO010000000
VERSION     DASRVO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55760"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /gene="accB"
                     /locus_tag="VFF05_01550"
     CDS             <1..397
                     /gene="accB"
                     /locus_tag="VFF05_01550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZW50521.1"
                     /translation="IKEGEEVVRLSRVPKNAAPVAAAPAVAPIAVAAAPAPVAAATAA
                     EAPPANALPAGHVVKAPMVGTFYASATPGTPAFVKVGQQVKAGETLGIIEAMKMFNQI
                     EADHAGTVVAVLAANGQPVEFDEPLFVIG"
     gene            405..1772
                     /gene="accC"
                     /locus_tag="VFF05_01555"
     CDS             405..1772
                     /gene="accC"
                     /locus_tag="VFF05_01555"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZW50522.1"
                     /translation="MLDKVVIANRGEIALRVLRACQTLGIKTVAVHSTVDRNLKHVGM
                     ADESVCIGPAPATESYLNVPAIIAAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVF
                     IGPTADVIRLMGDKVEAIRAMKAAGVPCVPGSGGSLGEDAAANAKIAREIGYPVIIKA
                     AGGGGGRGMRVVHTEAHLSNAITLTKSEAKAAFGNAEVYMEKFLENPRHVEIQVLADG
                     QGNAIHLGERDCSMQRRHQKVVEEAPAPDITPEQRAEIGKVCVDACVRIGYRGAGTFE
                     FLYENGRFYFIEMNTRIQVEHPVTELITGVDLVREQLLIAGGEKLSIKQSDIKINGHA
                     IECRINAEDPDTFMPSPGTVKRFEAAGGPGVRIDTHVYDGYRIPPNYDSMIGKLIVHG
                     RDRATAIARMRIALAEMVFEGVKTNLPLQQRIMADGGFHAGGQNIHYLEKRIAEAKEK
                     AIKLG"
     gene            1777..2673
                     /gene="prmA"
                     /locus_tag="VFF05_01560"
     CDS             1777..2673
                     /gene="prmA"
                     /locus_tag="VFF05_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZW50523.1"
                     /translation="MAWLELSLTISAAQQASVEAALEDLGALSITLQDADADTPDERA
                     IFEPGVGETPLWNEVVLQALFDANADRAGLVHALAELIPGIEPSHIAFREVADQDWTR
                     AWLDTFKPMRFGKRLWIYPWNVEAPNAADTVIVRLDPGLAFGSGTHPTTALCLEWLDS
                     ADLAGKTLVDYGCGSGILAIAALKLGAARAIGVDNDEQAIAASRDNAQRNGIVENLDL
                     YLPQHFPQMQSDAFVANILAGPLAELAALFAQSLKPGGALALSGILDGQQDELLAHYA
                     QWFDALRATTRDGWVRIDGTRR"
     gene            complement(2680..>3737)
                     /locus_tag="VFF05_01565"
     CDS             complement(2680..>3737)
                     /locus_tag="VFF05_01565"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW50524.1"
                     /translation="EHRGDVASSVHNLGFLAFHRSDYAQARDLYTRALAEKRAVYGND
                     DPRTLNTLDNLAQAEEELGDLDAAEHNLSDALALQVKVLGADSVAVARAHNDLASVQQ
                     DRGEYAAAETNYRAALDLYVRLEPANSMAWAVAANNLATLEEDRGDYAAALPLFRESL
                     RIRQAHYKAPSASVARVQNNLARCELELDELAAARPFIDAALATRRALKLDPAQLFDS
                     EMLDAQWLVRSGRTREAAAALHALKPPDGRGNYGRRARYAIVEAELAASHRDWKAALA
                     AQRRSLDALRGELGEQHPAYARLAAKAAEYAHAAHDDAAAKSLLQPALPVLRAALVEQ
                     AAQRHDAEKLAATLKLR"
BASE COUNT          658 a         1265 c         1222 g          592 t
ORIGIN      
        1 aatcaaggag ggcgaggagg tcgtgcgcct gtcgcgcgtg ccgaagaacg ccgcgccggt
       61 cgccgccgca ccagccgtcg cgccgatcgc ggtggccgcc gcgccggccc cggtcgccgc
      121 cgcgaccgca gccgaggctc cgccggcgaa cgcgctgccc gccggccacg tggtgaaggc
      181 gccgatggtc ggcaccttct acgcctcggc cacaccgggc acgcctgcct tcgtcaaggt
      241 cggccagcag gtcaaggccg gcgagacgct gggcatcatc gaggcgatga agatgttcaa
      301 ccagatcgag gccgatcatg ccggcaccgt ggtggcggtt ctcgccgcga acggccagcc
      361 ggtcgaattc gacgaaccgc tgttcgtgat cggctgacgc agtcatgctc gacaaagtcg
      421 tcatcgccaa ccgcggcgaa atcgccctgc gcgtgctgcg cgcctgccag acgctgggca
      481 tcaagaccgt ggccgtgcat tccacggtcg accgcaatct caagcacgtc ggcatggccg
      541 acgaatccgt gtgcatcggc ccggcgccgg caacggagag ctacctcaac gtgcccgcga
      601 tcatcgccgc ggcggaggtt accgacgcgc aggcgatcca cccgggttac ggattcctgt
      661 ccgagaacgc ggatttcgcc gagcgcgtgg agcaatccgg cttcgtcttc atcggcccga
      721 ccgcggacgt gatccgcctg atgggcgaca aggtcgaggc gatccgggcg atgaaagccg
      781 cgggcgtgcc ctgcgtaccc ggttcgggcg ggtcgctcgg cgaagatgcg gcagcgaacg
      841 cgaaaatcgc gcgcgaaatc ggctacccgg tgatcatcaa ggcggccggc ggcggcggcg
      901 gacgcggcat gcgcgtggtg cacaccgagg cgcacctgag caatgccatc accctgacca
      961 aatccgaggc caaggccgcg ttcggcaatg ccgaggtcta catggagaaa ttcctggaga
     1021 atccgcgcca cgtggagatc caggtgctgg ccgacggcca gggcaatgca atccatctcg
     1081 gcgaacgcga ctgctcgatg cagcgccgcc accagaaagt ggtcgaggaa gcgccagcac
     1141 cggacatcac gcccgagcag cgtgccgaga tcggcaaggt ttgcgtggat gcctgcgtgc
     1201 gcatcggcta ccgcggtgcc ggcacgttcg agttcctgta cgagaacggg cgcttctact
     1261 tcatcgagat gaacacgcgc atccaggtcg agcatccggt gaccgaactc atcaccggcg
     1321 tggacctcgt gcgcgagcag ctgctgatcg ccggcggcga gaagctgtcg atcaagcagt
     1381 ccgacatcaa gatcaacggc cacgccatcg aatgccgcat caatgcggaa gatccggaca
     1441 cgttcatgcc cagtcccggt acggtcaaac gcttcgaggc cgccggtggc cccggcgtgc
     1501 gcatcgacac gcacgtctac gacggctacc gcatcccgcc gaactacgat tcgatgatcg
     1561 gcaagctcat cgtgcacggc cgcgaccgcg cgaccgcgat cgcgcgcatg cgcatcgcgc
     1621 tcgcggagat ggtgttcgaa ggcgtcaaga ccaacctgcc gctgcagcag cgcatcatgg
     1681 ccgacggcgg ttttcacgcc ggcggccaga acatccatta cctggaaaag cgcatcgccg
     1741 aggcgaagga aaaagcgatc aagctgggct gacgcgatgg cttggctcga actctcgctc
     1801 accatttcgg ccgcgcagca ggccagcgtc gaagccgcgc tggaagacct tggcgcgctg
     1861 tcgatcacgc tgcaagacgc cgacgccgac acgcccgacg agcgcgcgat cttcgagccg
     1921 ggcgtcggcg aaacgccact gtggaacgaa gtcgtcctgc aagcactgtt cgacgcaaat
     1981 gccgatcgcg ccggactcgt gcatgcgctc gccgagctga ttcccggcat cgaaccttcg
     2041 catatcgcgt ttcgcgaagt ggccgaccag gactggaccc gcgcctggct cgatacgttc
     2101 aagcccatgc gcttcggcaa gcgtttgtgg atttatccat ggaacgtgga ggcgcccaac
     2161 gctgccgaca cggtgatcgt gcgcctggat cccggactgg cgttcggcag cggcacgcat
     2221 ccgaccacgg cgctgtgcct ggaatggctc gatagcgccg atcttgccgg caagacgctg
     2281 gtcgattacg gctgcggctc gggcatcctc gccatcgccg cgctcaagct cggtgccgcg
     2341 cgcgcgatcg gcgtggacaa cgacgaacag gccatcgccg ccagccgcga caacgcgcaa
     2401 cgcaatggca tcgtcgagaa tctggatttg tatcttccgc agcattttcc acaaatgcaa
     2461 tccgacgcgt tcgtcgcgaa catccttgcc ggcccgctcg cggaactcgc agcgctgttc
     2521 gcgcaaagcc tcaaacccgg cggcgcgctg gccctgtcgg gcatcctcga tggccaacag
     2581 gacgagctgc tcgcgcatta cgcgcagtgg ttcgacgcct tgcgcgcgac gacgcgcgat
     2641 ggatgggtca ggatcgacgg cacccgtcgt tgattggatc taacgcagct tcaaggtcgc
     2701 cgcgagcttt tctgcgtcgt gccgctgcgc cgcctgttcg accagcgccg cgcgcaaaac
     2761 cggcagggcc ggctgcaaca aggactttgc cgccgcatcg tcgtgcgccg catgcgcata
     2821 ttccgctgct ttcgccgcaa gccgggcata ggccggatgc tgttcgccca gttccccgcg
     2881 caacgcatcc agcgaccggc gttgcgcagc caacgccgcc ttccagtcgc gatgcgacgc
     2941 ggccaactcg gcttccacga tcgcataacg cgcacggcgc ccgtagttgc cgcgtccatc
     3001 tggaggcttc aacgcgtgca gcgcggcggc ggcttcgcgc gtgcggccgg atcgcaccag
     3061 ccactgcgcg tcgagcattt ccgaatcgaa caactgggcc ggatcgagct tgagcgcgcg
     3121 ccgcgtcgcc agcgccgcat cgatgaatgg acgcgcggcg gcaagctcgt ccagttccaa
     3181 ttcgcaacgc gccagattgt tctgcacgcg ggccaccgac gcgctcggcg ccttgtagtg
     3241 cgcctgccgg atgcgcagcg attcgcggaa caacggcagg gccgcggcgt aatcgccgcg
     3301 atcttcctcg agcgtggcga ggttgttcgc ggcgaccgcc caggccatcg agttggccgg
     3361 ttccaggcgc acgtaaagat ccagcgcggc gcgatagttc gtttcggcgg cggcatattc
     3421 gccgcgatcc tgctgcacgc tggccaggtc gttgtgcgcg cgtgcgaccg cgacgctgtc
     3481 ggcgccgagc accttgacct gtagcgccag cgcgtccgac aaattgtgtt ccgcggcgtc
     3541 gagatcgccg agttcttctt ccgcctgggc caggttgtcg agcgtgttga gcgtgcgcgg
     3601 atcgtcgttg ccgtaaacgg cgcgcttttc cgccaacgct cgggtgtaca gatcccgggc
     3661 ctgggcgtag tcgctgcgat gaaacgcgag gaagccgaga ttgtgcaccg acgaggcgac
     3721 atcgccgcga tgttcgg
//
LOCUS       DASRVO010000136         1218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_211412, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000136 DASRVO010000000
VERSION     DASRVO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211412"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VFF05_01570"
     CDS             <1..979
                     /locus_tag="VFF05_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW50525.1"
                     /translation="SASYSGTAALVADHEAQVTAKTSGVLKKLYVEEGMTVRAGQLLA
                     ELDDADARAKAKQAEAQMHKAEATFTHAQAAIPKHLIPEYEFEQDKFDLAAQRAAYEA
                     ANIQLSYTRIVAPVDGVIAERSIKIGNLVQANQNLFRIVGMNPLQAVLNVPERQLGIL
                     KAGQAVQLEADALPGKKFAGTILRIAPVVDPASGTFRVTCQFHDASAVLRPGMFARID
                     IVYDQRADALTVPRSALVEEDGQTSVFVVDKAPPAPAPKAEAGKPAAKAAVAAPNGFI
                     AHRQLVKTGYSDGDKVEIVSGLDAGARVITVGRNAVRDGTAVLVLDNKA"
     gene            976..>1218
                     /locus_tag="VFF05_01575"
     CDS             976..>1218
                     /locus_tag="VFF05_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW50526.1"
                     /translation="MSLVELATRRRVTVGMVTLTLVLFGLIALSGLKVNLLPDLTYPT
                     LTVRTAYEGAAPAEIETLISQPVEEALGVVKNVRAID"
BASE COUNT          245 a          387 c          383 g          203 t
ORIGIN      
        1 cagcgcgagc tatagcggaa cggctgcgct ggtggccgat cacgaagcgc aggtcaccgc
       61 gaaaacttca ggcgtcttga agaagctcta tgtcgaagag ggaatgaccg tgagagcggg
      121 gcaattgctc gccgaactcg acgatgccga tgcgcgggcc aaggccaagc aggccgaggc
      181 gcagatgcac aaggccgagg cgacattcac tcacgcacag gccgcgattc cgaaacactt
      241 gatccccgaa tacgaattcg aacaggacaa gttcgatctg gccgcgcagc gcgccgccta
      301 cgaggcggcg aacatccagt tgtcctacac ccgcatcgtc gcaccagtcg atggcgtgat
      361 tgccgagcgc agcatcaaga tcggcaatct cgtgcaggcc aaccagaacc tgttccgcat
      421 cgtcggcatg aatccgttgc aagccgtgct taacgtgccc gagcgccagc ttggaatcct
      481 gaaagcgggg caggcggtgc aactcgaagc cgacgcgctg ccgggcaaga aattcgccgg
      541 cacgatcctg cgcattgcgc cggtggtcga tccggccagc ggcacgttcc gcgtcacctg
      601 ccagttccac gatgccagcg cggttttgcg tcctggcatg ttcgcgcgca tcgacatcgt
      661 ctacgaccag cgcgccgatg ctttgaccgt gccgcgttcg gcattggtgg aggaggacgg
      721 ccagacttcc gtgttcgtgg tcgacaaggc accgcccgca cctgctccga aggccgaagc
      781 cggcaagcct gctgcaaagg cagcggttgc cgcgccaaac ggtttcattg ctcaccgcca
      841 actggtgaag accggctata gcgacggcga caaggtggag atcgtttccg gattggacgc
      901 aggcgcacgc gtcatcacgg tcggccgcaa cgccgtgcgc gatggcaccg ccgtattggt
      961 gctggacaac aaggcatgag cctcgtcgaa ctcgccaccc gtcggcgcgt caccgtcggc
     1021 atggtgacgc tcaccctggt tttgttcggc ctgatcgcgc tgtccgggct gaaggtcaac
     1081 ctgctgccgg acttgaccta tccaacgctg accgtgcgca ccgcgtacga aggcgccgcg
     1141 ccggccgaaa tcgaaacatt gatctcgcag ccggtcgagg aagctcttgg cgtggtgaag
     1201 aacgtacgcg ccatcgat
//
LOCUS       DASRVO010000137         1115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_315490, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000137 DASRVO010000000
VERSION     DASRVO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315490"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..653
                     /locus_tag="VFF05_01580"
     CDS             276..653
                     /locus_tag="VFF05_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZW50527.1"
                     /translation="MATKKTAKKVAPKSATKPVAPKPVKVALGKSGLVEYITDACGVM
                     AKDVRAVLAALEGAVVGSVNKNGARTFTLPGLLKITVVKLPAKPKRKGINPFTKQEQW
                     FAAKPASVKVKVRPLKKLKDAAA"
     gene            complement(871..>1115)
                     /locus_tag="VFF05_01585"
     CDS             complement(871..>1115)
                     /locus_tag="VFF05_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HZW50528.1"
                     /translation="FIRNGVEYDAAKAADHLRLERRYAGSRVTTAEDFIVCCATGSSI
                     TGEKYRIRLHDGRTVDAAAFLRDRLAARAALDRTGG"
BASE COUNT          219 a          355 c          356 g          185 t
ORIGIN      
        1 tccggagccg gcgccaacgg ttatcaggtc gaagtcgtag ctggacattg gacggcaccc
       61 ggagcgatcg atgccgccga atatgaccta ttttgccttg cgcgagcagc cggtcatgcg
      121 aaaaaaaacc gtcggcgcgc ctgttccggg gttccttcgc gacgcgcgga gccgtagcat
      181 gcgcgtctgt cgcgactttc ggtcccgaca agctctggcg gcgcagacag cgtatgcttc
      241 gcgttgcttg atccatcaac catctgggaa aaaaaatggc taccaagaaa actgcaaaga
      301 aagtcgcacc aaagtcggca accaagccgg ttgcacccaa gccggtcaag gtagccctcg
      361 gcaagtccgg gctggtcgag tacatcaccg atgcctgcgg cgtgatggcc aaggacgtcc
      421 gcgcggtact ggctgcgctc gaaggcgccg tcgtcggttc ggtcaacaag aacggcgccc
      481 gcacgttcac gcttccggga ctgctgaaga tcaccgtcgt caagctgccg gccaagccga
      541 agcgcaaggg catcaatccg ttcaccaagc aggaacagtg gttcgcggcc aagcctgcca
      601 gcgtcaaggt caaggtgcgc ccgctgaaga agctcaagga cgccgcggcc tgatggcgca
      661 ttcgccggcc tgagccggcg tttcgtccgc gaacagcagc caagttgctg gcgcggaccg
      721 gagcgcggtg caggcggtgt agccgtcagc gagaaacacc ggccgcgcgc gcatggcgcg
      781 cggccagtga cagtttcggc gtgatgcgac gtgaccgtcg gcgccgacgc ggcgccagcc
      841 acgtcattgc gtcatgcagg atgtccgtga tcagccgccg gtccggtcca atgccgcgcg
      901 cgcagcaagt ctgtcgcgca gaaatgcggc tgcatcgacc gtcctgccat cgtgcaagcg
      961 gatccggtac ttttccccgg tgatcgaaga tccggtcgcg caacagacga tgaaatcctc
     1021 cgccgtcgtt acccggcttc cggcataacg ccgctcgagg cgcaggtgat cggccgcctt
     1081 cgcggcgtcg tattcgacac cgttgcgaat gaagt
//
LOCUS       DASRVO010000138         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_341495, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000138 DASRVO010000000
VERSION     DASRVO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341495"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1028
                     /locus_tag="VFF05_01590"
     CDS             <1..>1028
                     /locus_tag="VFF05_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HZW50529.1"
                     /translation="PILEWDKRNLEGKVTVMGVVEAAKKYVPEVRAAGADIVIAIVHG
                     GINTAPYTSRMENADWYLAQVPGIDAMLTGHSHQVFPDPGNAKSHYAHMHDVDNERGF
                     VNGVPTVMGDFFGKAIGVIDLHLTYAIGHWQVDKDATHSEVRLVEPRGKSAVEPDEEI
                     AQMVAPEHAATIAYVRTPIGRSDFPMTTYFVAAGDTSALTLVNTAQRDYVETYINTNL
                     PQFRDVAVLSAAAPFKAGFGGPNDYTDVPSGPLAINNAADLYLYPNTLTAVKITGAGV
                     KAWLEKSAQWFNRIDPRDAKPQELINRRYPTYNFDVLQGDSSGGGLTYAIDVTKPVGE
                     RVVDLRYR"
BASE COUNT          215 a          341 c          304 g          168 t
ORIGIN      
        1 cgccgatcct ggaatgggac aaacgcaacc tcgaaggcaa ggtcacggtg atgggcgtgg
       61 tcgaggcggc gaagaaatac gtgccggaag tccgcgccgc cggcgccgac atcgtgatcg
      121 ccatcgtcca cggcggcatc aacaccgcgc catatacgtc ccgaatggaa aatgccgact
      181 ggtacctggc ccaggtgccc ggcatcgacg ccatgcttac cggccattcg caccaggttt
      241 ttcccgatcc cggcaatgcg aaatcgcatt acgcgcacat gcacgacgtg gacaacgaac
      301 gtggtttcgt caacggcgtg ccgaccgtca tgggcgattt tttcggcaag gccatcggcg
      361 tgatcgacct gcacctcacc tacgccatcg gccactggca ggtggacaag gacgcaacgc
      421 actcggaagt gcgcctcgtc gaaccgcgcg gcaagtccgc cgtcgaaccc gacgaggaaa
      481 tcgcgcagat ggtcgcgccg gaacacgcgg ccactatcgc ctacgtcagg acaccgatcg
      541 gacgcagcga ttttcccatg accacgtact tcgtcgccgc aggcgatacc tcggcgctga
      601 cgctggtcaa caccgcgcag cgcgactatg tggaaaccta tataaatacc aatctgccgc
      661 aattccgcga cgttgccgtg ttgtccgccg ccgcgccgtt caaagccggt ttcggcggac
      721 cgaacgacta caccgacgtg ccgtccgggc cgctcgcgat caacaacgcg gccgacctct
      781 acctgtatcc gaacaccttg accgcggtga agataaccgg cgccggggtg aaggcctggc
      841 tggagaaatc cgcgcagtgg ttcaatcgca tcgatccgcg cgatgcgaaa ccgcaggaac
      901 tcatcaaccg gcgttatccg acctacaact tcgacgtgtt gcagggcgat tccagcgggg
      961 gcggcctgac ctacgccatc gacgtgacca agccggtcgg cgagcgcgtc gtggatttgc
     1021 gctatcgc
//
LOCUS       DASRVO010000139         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_185910, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000139 DASRVO010000000
VERSION     DASRVO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185910"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /gene="pgeF"
                     /locus_tag="VFF05_01595"
     CDS             <1..484
                     /gene="pgeF"
                     /locus_tag="VFF05_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZW50530.1"
                     /translation="DAAFTSESGVVLAIQTADCLPVLLCADDGAQIAAIHAGWRGLAG
                     GVIEACVRRLATKPSRLLAWLGPAIAAQSYEVGDEVRKAFLAHAAAAAAAFAETRAGH
                     WRCDLSALARMRLADLGVERVFGGGFDTFSDSRFYSYRRDAGCSGRMASLVWKTGMDG
                     "
     gene            complement(486..>1050)
                     /locus_tag="VFF05_01600"
     CDS             complement(486..>1050)
                     /locus_tag="VFF05_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HZW50531.1"
                     /translation="ESHPPLHGRVSLLMISVPAAREITIYRALQSQIEQAVGRINGRH
                     ARLDWSPVRFFARSFPFEEVLAYYAAADIMWITPLRDGLNLVAKEFVATQGLDDGNGI
                     LVLSEFAGAAAELKGALLTNPHDPADLSAVLARALEMLPAEATGRLRQLYDIVTHYDV
                     RRWGQDFLAAVARRAPATTSRQALGKS"
BASE COUNT          170 a          342 c          351 g          187 t
ORIGIN      
        1 cgatgccgcg ttcacgtccg aatccggggt ggtgctcgcg atccagaccg ccgattgcct
       61 gccggtcctg ctgtgcgcag acgacggggc acagattgcc gcgattcacg ccggttggcg
      121 cggactcgcc ggcggtgtga tcgaagcgtg cgtacggcgt ctggcgacaa agccgtcgcg
      181 tctgctcgcg tggctcggtc cggcgatcgc ggcgcaatcc tacgaggttg gcgacgaggt
      241 gcgtaaggcc tttctcgcac acgctgcggc agcggcagcg gcgttcgccg aaacacgggc
      301 gggccattgg cgttgcgatc tcagcgcgct ggcgcgcatg cgcttggccg acctgggcgt
      361 cgaacgcgtt ttcggtggcg gtttcgacac gttttccgac tcgcgttttt actcgtatcg
      421 ccgcgatgcg ggttgcagcg gacgcatggc gagtctcgtg tggaaaaccg gaatggacgg
      481 ttgattcagg acttgcccag cgcctgacgc gatgtcgtgg caggcgcccg gcgtgccacc
      541 gcggcaagaa aatcctgtcc ccagcgacgc acgtcataat gcgtgacgat gtcgtacaac
      601 tggcgcagcc ggcccgtcgc ctcggccggc aacatttcca gcgcgcgcgc cagcaccgcg
      661 ctcagatctg ccggatcgtg cggattggtc agcagcgcgc ccttgagttc ggcggccgcg
      721 ccggcgaatt cggacagcac cagtatgccg ttgccgtcgt ccagaccctg ggtcgccaca
      781 aattccttgg ccaccagatt cagcccgtcg cgcagcggag taatccacat gatgtcggcc
      841 gccgcgtaat aggccagaac ctcctcgaac gggaagctgc gcgcgaagaa gcgcactgga
      901 ctccagtcca gacgcgcatg gcggccgttg atgcgcccca cggcttgctc gatctgcgat
      961 tgcaatgccc ggtagatggt aatctcgcgc gccgccggca cgctgatcat cagcaacgat
     1021 acccggccat gcagcggcgg gtgcgattcg
//
LOCUS       DASRVO010000140         1545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_55910, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000140 DASRVO010000000
VERSION     DASRVO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55910"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFF05_01605"
     CDS             complement(<1..294)
                     /locus_tag="VFF05_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HZW50532.1"
                     /translation="MRRVVVTGMGLVSCLGHGLDQVAHALRDGLSGIRAMPEFAAHGL
                     RSTVAGQPEIDLEALIDRKLKRFMGDAAAYAYVSMCNAIADAGLALDRIRHPRI"
     gene            complement(296..811)
                     /gene="fabA"
                     /locus_tag="VFF05_01610"
     CDS             complement(296..811)
                     /gene="fabA"
                     /locus_tag="VFF05_01610"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HZW50533.1"
                     /translation="MTRPASLDFEKLLACARGEMFGPGNARLPSPPMLMFDRITHIDE
                     HGGAHGKGVLHAELDIRPDLWFFGCHFEGDPVMPGCLGVDAMWQLAGFFLPWLGEAGR
                     GRALGVGEVKFTGQVLPTARLVRYEIDVSRVIRGRLKLVVADGRTFVDDRLIYAANDL
                     RVGLFQSTENL"
     gene            complement(953..1297)
                     /locus_tag="VFF05_01615"
     CDS             complement(953..1297)
                     /locus_tag="VFF05_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZW50534.1"
                     /translation="MDQSMYPPPTAEIAHKRRELAPKQLDAFKAFSAAVFAEGALPVR
                     TKQLIAVAVAHVTQCPYCIRGHTEAALRHGASEQEIMESIWVAAEMRAGGAYAHSALA
                     IDTMHAHGAGQK"
BASE COUNT          284 a          493 c          503 g          265 t
ORIGIN      
        1 aatgcgcggg tgacggatgc gatccagcgc gaggccggca tcggcgatcg cgttgcacat
       61 ggacacgtag gcatacgcag ccgcatcgcc catgaaacgc ttgagcttgc ggtcgatgag
      121 cgcttcgagg tcgatctcgg gctgaccggc caccgtgctg cgcaggccgt gcgcggcgaa
      181 ttccggcatc gctcgaatcc cgctcaggcc atcgcgcagc gcatgcgcga cctgatccaa
      241 cccatgtccg agacaggaca ccaggcccat gccggtaacg acgacgcggc gcatgtcaga
      301 gattctccgt ggattggaac aggcccacac gcaaatcgtt ggctgcgtag atcaggcgat
      361 cgtcgacgaa cgtgcggcca tcggcgacga ccagtttcag acgcccgcgg atgacccggc
      421 tgacatcgat ctcatagcgc acgaggcgcg ccgtgggcag cacctgcccg gtgaacttga
      481 cttcgcccac gccgagggcg cggccgcgcc cggcttcgcc aagccagggc aggaagaagc
      541 cggccagctg ccacatcgca tccacaccca ggcagccggg catgaccgga tcgccttcga
      601 aatggcagcc gaaaaaccac aggtctggac ggatatcgag ttccgcatgc agcacgccct
      661 tgccatgtgc gccaccgtgc tcgtcgatgt gggtaatgcg atcgaacatc agcatcggcg
      721 gcgatggcaa acgcgcgttg cccgggccga acatctcgcc acgggcgcag gcgagaagtt
      781 tttcgaaatc gagcgatgcg ggtcgggtca tgatggcaac cggaagcgaa cgcgtagttt
      841 acggtgctgg gccggtgtgc gccacgactc aagtcaagac ccgtgcgccg cacacgcgcg
      901 catgctcctg gcgaagcggc gacgcaatcg ctgcattcgg tcgtgcgcga gcttactttt
      961 gtcctgcgcc atgcgcgtgc atcgtgtcta tcgccagtgc cgaatgcgca taggctccac
     1021 ctgcgcgcat ttccgcagcg acccagatcg actccatgat ttcctgctcg ctggcgccgt
     1081 gacgcaatgc ggcctcggtg tggccgcgaa tgcagtatgg gcattgggtg acatgcgcga
     1141 ccgcaaccgc gatcagctgc ttggtcctga ccggcagcgc gccctcggcg aagacggcgg
     1201 cactgaatgc cttgaaggcg tccagttgct tgggcgccag ttcgcggcgc ttgtgcgcga
     1261 tttcggcagt gggtggcggg tacatggact ggtccatgag gaaatctcct tgtgcatgcg
     1321 acgcgttcag cgcgccggct gtgtcgcgga tcggacacga agtgcctgaa atcgcggcgc
     1381 ttcaaagtcc cgcgttgttg cgatccagca cacgctcggc gcggtaactc gagcgcacca
     1441 gcggtcctgc gacggcttcc aggaaccctt tcgccagcgc cagttcgcgg taatgccgga
     1501 attcgtccgg ggtgacgaag cgttccaccg gcagatgatg ttcgg
//
LOCUS       DASRVO010000141         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_159722, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000141 DASRVO010000000
VERSION     DASRVO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159722"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFF05_01620"
     CDS             <1..417
                     /locus_tag="VFF05_01620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017162648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW50535.1"
                     /translation="LPGIVFHVIGSKAPKAILELAHDGVQVHGFVEDIAPFMEGCRLA
                     LAPLRYGAGVKGKINMAMSHGLPVVATTPAAEGMHARDGEDLLVADAPEDFAAAIVRA
                     YGDAALWKKLSDNGLANVREHFSFNAARAALRRVLG"
     gene            510..875
                     /locus_tag="VFF05_01625"
     CDS             510..875
                     /locus_tag="VFF05_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZW50536.1"
                     /translation="MTNVPVNFKDKLARFSEHWSPRVIAEMNDYQFKLAKIQGEFVWH
                     SHAETDEAFIVLEGAMTLEFRDRSVALSAGEMYVVPKGVEHRPLASRECCIMLVEPRG
                     VVNTGEAGGAYTAKNDVWA"
     gene            complement(964..>3263)
                     /locus_tag="VFF05_01630"
     CDS             complement(964..>3263)
                     /locus_tag="VFF05_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZW50537.1"
                     /translation="RNVVGVHDVGRAGEYHYIAMEYLGGGAVLANDGKPRPVGFALRV
                     TREIATALNYAHAKGFVHRDVKPDNILLREDGSAALTDFGIARASDSATHMTRTGTVI
                     GTPHYMSPEQARGRPLDGRADLYSLGIVLHELLTGRVPFHADDSLAVGIMHITQPIPI
                     LPERLYALQPLLNRLLAKNPEDRHQDGAAVADAIEQIELGIARGDYPELTDSSAPVVR
                     RDTSADAGTRVLTPAPAGGVRHRAEPSLGRMDDIANAPMHRSQPSRATAPQGRSRRWI
                     FAAIAVIVIAVLAAWFFQNRLRGLLPNTELNTLIARGDKALADGNLVGNHNDSARELF
                     QAARTLDPDNDAARAGLNNVGERLIEAARAALTRNDFSTAQSDLDQANEVLGGGKEVE
                     DLKAALHAAQTRNTASASLLAKADAALAAGKLVGSGSAAEAFQRVLDADMTNGLALNG
                     LKKVAEAVAQQARDAIAANNVDQANQLIAELAQLSPNHPAIPELRGAMAKLHAGVEQA
                     LEDTLSRAEAQQRAGRIAGADGALTLFQSALKSDPANARAKSGMAKIAQTLIAQANAA
                     LDDDNATQAAALLQQAAQAAPDSPALRKANARLGDARERLDAAKRKAQLTPADLARIQ
                     QMLAAANQAMTAGNLILPPGDCAFDKFRAVLAIDADNAAAMEGLAKIPARAKELFEQA
                     IRGGTPNKARAYIDAVAESDPGDTSVSMMRSRLADVFLDETQSFIAQKRRAEAGRALD
                     SARELNPNNPRLAALRAQFESMSVN"
BASE COUNT          509 a         1076 c         1107 g          571 t
ORIGIN      
        1 ctgcccggca tcgtcttcca cgtgatcggc agcaaggcgc caaaagccat cctggaactc
       61 gcccacgacg gcgtgcaggt gcacggcttc gtcgaggaca tcgcaccgtt catggaaggc
      121 tgccgcctcg cgctggcgcc gctgcgttac ggcgccggcg tcaagggcaa gatcaacatg
      181 gcgatgagcc acggcctgcc ggtggtggcg acaacgcccg cggccgaagg catgcacgcg
      241 cgcgatggcg aagacctgct cgtcgccgat gcgccggaag atttcgccgc cgccatcgtg
      301 cgcgcctacg gtgatgcggc attgtggaag aagctctctg acaacggcct cgccaacgtg
      361 cgcgagcact tctcgttcaa tgcagcgcgc gcggcgttgc gcagggtcct gggataagcg
      421 gcgcgtttcg ctcgagcgaa cggctcgtgg ccggttgcgc taccatcgcg ccaacgcggc
      481 agccgccgca tgacccgggc caggcgcgca tgaccaacgt ccccgtaaac ttcaaggaca
      541 agctggcccg gttctcggag cattggtcgc cacgcgtcat tgccgaaatg aacgactacc
      601 agttcaagct cgccaagatt cagggtgaat tcgtctggca ctcccacgcg gaaacggatg
      661 aagcattcat cgtccttgaa ggcgcgatga cgctggagtt ccgcgatcgc agcgtcgcgc
      721 tttccgcggg cgaaatgtat gtcgtgccga agggcgtcga acaccgtccg ctggcatcga
      781 gggaatgttg catcatgctc gtagagcctc gcggcgtcgt caacaccgga gaggcgggcg
      841 gcgcatacac cgcaaaaaac gacgtatggg cctagcgcac ctgccattcg ctcaagcgga
      901 cggccggaga tcggcaacgg caagccggca ggtagcgcgg ccatcgccgc cttcctgcag
      961 gcgctaattc accgacatcg actcgaactg cgcgcgcaag gccgccaggc gcggattgtt
     1021 cggattcaat tcgcgcgccg aatcgagcgc gcggccggct tcggcacggc gcttctgcgc
     1081 gatgaaactc tgcgtctcat caaggaacac gtcggcgagg cggctgcgca tcatcgacac
     1141 ggaagtatcg ccgggatcgg attcggccac agcgtcgatg taggcgcgcg ccttgttcgg
     1201 cgtgccgccg cggatcgcct gctcgaacaa ttccttggcg cgggcgggaa tcttcgccaa
     1261 gccttccatc gccgccgcgt tgtcggcatc gatggcgagt accgcacgga atttgtcgaa
     1321 ggcgcaatcg ccgggcggca atatgagatt gcccgccgtc atcgcctgat tcgccgcagc
     1381 aagcatctgc tggatgcgcg ccaggtccgc gggagtgagc tgcgccttgc gcttggccgc
     1441 atcgaggcgt tcacgcgcat cgcccagccg cgcattcgcc ttgcgcaggg ccgggctgtc
     1501 cggcgcggcc tgagcggcct gctgcaacag ggcggcggcc tgcgtcgcgt tgtcgtcgtc
     1561 gagcgcggcg ttggcctgag cgatcaacgt ctgcgcaatt ttggccatgc cgcttttcgc
     1621 gcgcgcattc gctggatcac tcttcagcgc ggactgaaac agcgtgagcg caccgtccgc
     1681 accggcgatg cgtccggcgc gttgctgcgc ctcggcgcgc gaaagcgtgt cttccagcgc
     1741 ctgctccaca ccggcgtgca gcttcgccat agcgccgcgc agctcgggaa tcgccggatg
     1801 attcggcgag agttgcgcca gctcggcgat gagctggttg gcctggtcca cgttgttcgc
     1861 ggcgatcgcg tcgcgcgcct gctgggccac cgcttcagcg acttttttca gcccgttcaa
     1921 cgccagcccg ttcgtcatgt cggcatcgag tacgcgctgg aaggcttcgg cggcgctgcc
     1981 gctgccaacg agtttgccgg ccgcgagcgc ggcgtcggcc ttggcgagca ggctcgcgct
     2041 ggccgtgttg cgcgtctgcg cggcatgcag ggcggccttg agatcctcga cttccttgcc
     2101 gccgccaagt acctcgttgg cctgatccag gtccgactgc gcggtggaaa aatcgttgcg
     2161 cgtgagcgcc gcgcgtgcgg cttcgatcag gcgttcgccg acattgttca gacccgcgcg
     2221 cgccgcatcg ttgtccggat cgagcgtgcg cgcggcctgg aacagttcgc gcgcgctgtc
     2281 gttgtggttg ccgaccaggt tgccatcggc gagcgccttg tcgccgcgcg cgatcagggt
     2341 attcaattcg gtgttcggca gcagcccgcg caggcgattc tggaaaaacc acgccgccag
     2401 caccgcgatg acgatgacgg cgatcgctgc gaaaatccaa cgccggctcc gcccttgcgg
     2461 cgcagtggcg cgcgagggtt gcgaacgatg catcggcgca ttggcgatgt cgtccatccg
     2521 ccccagactc ggttccgcgc gatggcgcac gccacccgcc ggggcgggtg tgaggacgcg
     2581 cgttccggcg tccgcgctgg tgtcgcgccg cacgaccggt gccgacgaat ccgtcaattc
     2641 cggataatcg ccgcgggcga tgcccagttc gatctgctcg atcgcatccg ccaccgcagc
     2701 gccgtcctga tggcggtctt ccggattctt ggcgagcagt cggttgagca gcggctgcag
     2761 cgcatacagg cgctcgggca ggatcggaat cggctgggtg atgtgcatga tgccgacggc
     2821 gagcgaatcg tcggcgtgga atggcacgcg cccggtcagt agttcgtgga gcacgatgcc
     2881 aaggctgtac agatcggcac gtccgtcgag cggacgcccg cgtgcctgtt ccggactcat
     2941 gtaatgcggc gtgccgatca ccgtgccggt gcgggtcatg tgcgtggccg aatcgctggc
     3001 gcgcgcgatg ccgaagtcgg tcagcgccgc gctgccgtcc tcgcgcaaca ggatgttgtc
     3061 gggcttgacg tcgcggtgca cgaaaccctt ggcgtgcgcg tagttcagcg cggtcgcaat
     3121 ctcgcgcgtc acgcgtaggg cgaagccgac cggacgcggc ttgccgtcgt tggccagcac
     3181 ggccccgccg ccgaggtact ccatcgcgat gtagtggtat tcgccggcgc gaccgacgtc
     3241 gtgcacgccg accacgttgc ggt
//
LOCUS       DASRVO010000142         1415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_107829, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000142 DASRVO010000000
VERSION     DASRVO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107829"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1059)
                     /locus_tag="VFF05_01635"
     CDS             complement(373..1059)
                     /locus_tag="VFF05_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZW50538.1"
                     /translation="MSATVPRVMIVDDEAPIVRVLKPSLAAAGYDVVEADTGRDALAR
                     VGQQIPDVILLDLGLPDMDGKDVILALRKWTQVPIIVLSAREAEAERIAALDTGADDY
                     VVKPFHTGELLARVRAALRHQHSRTELSQYSGNGLEIDFESRRVAIGGLEIHLTRKEY
                     DLLRTLARHAGQVVTHKQLLAAAWGGSVTDTQFVRVYIGQVRQKIERDPSNPRLILTE
                     AGIGYRLAAD"
     gene            complement(1056..>1415)
                     /locus_tag="VFF05_01640"
     CDS             complement(1056..>1415)
                     /locus_tag="VFF05_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW50539.1"
                     /translation="DPVLIEQALMNVIENAAAHAPAATPIMTSVQTLDAGVRLSVEDA
                     GEGISAADLPYVFEKFFRGGGDRKRSSGVGLGLAVTRGLIEAFGGSVRAVSPVRDGYG
                     TRIEIDLPAHRIEETGA"
BASE COUNT          240 a          473 c          452 g          250 t
ORIGIN      
        1 gcgcgttggc cgggccggct cttcgcatcg tgctgtcgca cggcaatctc gtcagcgaga
       61 agtagccggt tctgaaattg tcgatcgaca aaccacgccg atgtcgcgca actggcggaa
      121 cgtcccggca gtcagcgtag cctgctcgag gataccgctc tcggtgatct cgatcgtcag
      181 ccgcgacggc cggagcttgg cgtcggccag cgcattcgcg gttcgccctg gatcgcacgg
      241 acaaaaaaag agcgcccgaa ggcgcccttt gtccccaagt ctccccagcg gagaacggtg
      301 tcgaaccgtg gggaaactct aggcgcgttt gcgttcgagc acgagtgtaa ggattttata
      361 aggccacggg tttcaatccg cggccagccg gtacccgatg ccggcttcgg taaggataag
      421 gcgcggattg ctcggatcgc gttcgatctt ctgccgcacc tgaccgatgt agacgcgcac
      481 gaactgcgta tcggtgacgc tgccgcccca ggcggcggcg agcagttgct tgtgcgtgac
      541 cacctgcccg gcatgccgcg ccaaggtacg caacagatcg tactccttgc gcgtgagatg
      601 gatttccagg cccccgattg cgacgcgccg actttcgaaa tcgatctcca ggccattgcc
      661 cgaatactgc gacaattcgg tgcggctatg ctggtggcgc agcgccgcgc gcacgcgcgc
      721 gagcagttcg ccggtatgga acggcttgac cacatagtcg tccgcgccag tgtccagcgc
      781 ggcaatgcgc tcggcttccg cctcgcgcgc cgacagcacg atgatcggta cctgcgtcca
      841 cttgcgcaaa gcgaggatca cgtccttgcc gtccatgtcg ggcaggccca gatcgagcag
      901 gatcacgtcg ggaatctgct gcccgaccct ggccaatgcg tcgcgcccgg tgtcggcttc
      961 gaccacgtcg taaccggccg ccgcgagcga aggcttgagc acacgcacga tcggcgcctc
     1021 gtcgtcgacg atcatcacgc ggggcacggt tgcgctcatg cgccagtttc ctcgatgcgg
     1081 tgtgcgggca ggtcgatctc gatgcgcgtg ccgtagccat cgcgcaccgg actcactgcg
     1141 cgcacgctac caccgaacgc ctcgatcagg ccacgcgtga ccgccaggcc cagacccacg
     1201 ccggagctgc gtttgcggtc gccgccgccg cgaaagaatt tttcgaacac gtacggcagg
     1261 tcggcggcgg aaatgccttc gccggcgtct tcgacggaca gccgcacgcc cgcgtccagc
     1321 gtctgcacgc tcgtcatgat cggcgtggcc gccggtgcgt gcgcggcggc gttctcgatg
     1381 acgttcatga gcgcctgctc gatcagcacg ggatc
//
LOCUS       DASRVO010000143         1259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_263665, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000143 DASRVO010000000
VERSION     DASRVO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263665"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFF05_01645"
     CDS             <1..320
                     /locus_tag="VFF05_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50540.1"
                     /translation="ARAMDSPPFVNAWLGYAYAASGDRAHALAIIDEMNKNSLRGYVP
                     PFNLAIVHLGLGDRALALDYLEKADAADSQWLIYLGGDRIFDPLRAEPRFVALLRKKG
                     FVK"
     gene            367..>1259
                     /locus_tag="VFF05_01650"
     CDS             367..>1259
                     /locus_tag="VFF05_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50541.1"
                     /translation="MRLIAELKRRNVIRMAGLYLVGAWLIVQVAGTVLPMFGAPEWIG
                     RSFVVMLAIGFVPALVFAWVFELTPQGMKRDAEVRPEESIAPQTARRMDRMIIVALII
                     ALAYFAFDKFVPEKGSEPFSGDRSVEPTQRRVPEKESDPAPKSIAVLPFVNMSGDAGN
                     EYFSDGITEEILNALAQIADLKVAARTSAFAFKGKDPDLRKVGEMLDVATVLEGSVQR
                     AGDQVRITAQLIDARSGYHLWSEKYDRKLTNVFAIEDEISKAIAGKLQVQLAGGHADG
                     AGTTANPQAHELYLRGLTLLSA"
BASE COUNT          231 a          394 c          417 g          217 t
ORIGIN      
        1 aggccagggc catggattca ccgccgttcg tcaacgcatg gctcggctac gcgtacgccg
       61 cttccggcga tcgcgcgcac gcgctggcga tcatcgacga aatgaacaag aattcgttgc
      121 gcggctacgt gccgccgttc aacctggcga tcgtccacct cggactgggc gatcgcgcgc
      181 tggcgctgga ttacctggaa aaagccgatg ccgcggattc gcaatggctg atctacctgg
      241 ggggcgaccg gatcttcgat ccgttgcgcg ccgagccgcg cttcgtcgcg ctgctgcgaa
      301 aaaagggctt cgtcaagtga aacggcgcct gcatcgcgac gttgaattcg ggcaaggaat
      361 gcggcgatgc gcctgatcgc cgaactcaag cgccgcaacg tgatccgcat ggccggcttg
      421 tacctggtcg gcgcgtggct gatcgtgcag gtagccggga cggtgctgcc gatgttcggc
      481 gcgccggagt ggatcggacg cagcttcgtc gtgatgctcg cgatcggttt cgttccggcg
      541 ctggtgtttg catgggtttt cgaactgacc ccgcaaggca tgaaacgcga cgcggaggtc
      601 aggcccgagg aatcgatcgc gccgcagacc gcgcggcgca tggaccgcat gatcatcgtc
      661 gcgctgatca tcgcgctcgc gtatttcgcc ttcgacaagt tcgtgccaga aaaggggtca
      721 gagccctttt ccggtgatcg gtcggtcgaa ccaacccagc gtcgcgtacc ggaaaaggaa
      781 tctgaccccg cgcccaaatc catcgccgtg ctgccgttcg tcaacatgag cggcgatgcc
      841 ggcaacgagt acttcagcga cggcatcacc gaggagatcc tcaacgcgct ggcgcagatt
      901 gccgacctga aggtcgccgc gcgcacctcg gcgttcgcgt tcaagggcaa ggatccggac
      961 ctgcgcaagg tcggcgagat gctcgatgtc gcgaccgtgc tcgaaggcag cgtgcagcgc
     1021 gccggcgacc aggtgcgcat caccgcgcag ctgatcgacg cacgcagcgg ctatcacctg
     1081 tggtcggaga agtacgaccg caagctgacg aatgtgttcg cgatcgagga cgagatctcg
     1141 aaggcgatcg ccgggaagct gcaggtgcaa ctggcaggcg gtcatgccga tggcgccggc
     1201 accacggcca atccgcaggc gcacgagctt tacctgcgcg gactgaccct gctctcggc
//
LOCUS       DASRVO010000144         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_237314, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000144 DASRVO010000000
VERSION     DASRVO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237314"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFF05_01655"
     CDS             <1..554
                     /locus_tag="VFF05_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZW50542.1"
                     /translation="IALNALNPGVRVDTVAQRLLAQNVEDLIRDHDVVIDGADNFPTR
                     YLLDAACRRLRIPLVYGAVHRFTGQVSVFDARRDDSPCYRCLFPEPPAAADAPNCAEA
                     GVLGVLPGIIGLLQANEAIKLLLGIGHPLIGRLLCFDALAGTFRELRLPRDPQCPGCG
                     ANAKFSGYQDIEQVCSAHPGNAE"
     gene            complement(683..991)
                     /locus_tag="VFF05_01660"
     CDS             complement(683..991)
                     /locus_tag="VFF05_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HZW50543.1"
                     /translation="MNANNTVEIEAAAFRRLLQHLNVERSDVQNIDLMILAGFCRNCL
                     ADWYREAAAERGVEIGKDAARERIYGEPYAEWKAKYGKEATQEQLFALALAETKHPTS
                     "
     gene            1097..1945
                     /gene="folD"
                     /locus_tag="VFF05_01665"
     CDS             1097..1945
                     /gene="folD"
                     /locus_tag="VFF05_01665"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZW50544.1"
                     /translation="MAARILDGKRVADELLDSLRARVAQRVARGKSAPGLAVLLVGDD
                     AASAVYVRNKRRACRHVGFRSRDYDLPASTTQAELAALIDKLNADPQIHGILVQLPLP
                     ARIDANALINRIDPRKDVDGFHPENIGRLALRQRGLRPCTSKGVMTLLAHTDRPVRGQ
                     SAVVVGVSNHVGRPLAFELMLAGCTTICCHKFTRDLAHEVGEGDIVIVAAGKPGLVKG
                     AWIKPGAVVIDVGINRLADGSLAGDVEFAPAAERASWITPVPGGVGPMTVATLMENTL
                     EAAELS"
     gene            2013..2885
                     /locus_tag="VFF05_01670"
     CDS             2013..2885
                     /locus_tag="VFF05_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HZW50545.1"
                     /translation="MISNPLPMRRLRFAVLLAGVLVAACGSVMRKATDQFADNLTTAI
                     LNEDDPGTVRDGVPAYLLLIDGMIQGDPENAGALLAGAKLYGAYAGGFVSDPARTQRM
                     AGRAYDYAHHALCLREKDLCEALDKPYDAYVAELARTGRGDVPVVYGFAAAWAGKIQV
                     NSGDWNAIADLPKLQSTLERCTTLDPHYDNGGAYLYLGVINSIRPASLGGKPEQGKAY
                     FEKALELSGGKNQMVRVLYAQFYARLVFDKDLHDKLLNEVLAADPHAPGLTLINTLAK
                     EKAKALLESGKDYF"
BASE COUNT          514 a          989 c          936 g          565 t
ORIGIN      
        1 gcattgcatt gaatgcgctc aatccgggcg tacgcgtcga caccgttgcg cagcgcttgc
       61 tcgcgcagaa cgtcgaagac ttgatccgcg atcacgacgt ggtgatcgac ggcgctgaca
      121 atttcccgac gcgttatctg cttgatgccg cctgccggcg cctgcgcatt ccgctcgtct
      181 acggcgccgt gcatcgcttc accgggcagg tcagcgtgtt cgacgcgcgc cgcgacgatt
      241 cgccctgcta ccgctgcctg tttccggagc cgcccgctgc ggcggacgcg ccgaactgcg
      301 ccgaggccgg cgtgctgggc gtgttgcccg gcatcatcgg cctgctgcag gcgaacgagg
      361 cgatcaagct cctgctcggc atcggccatc cgctcatcgg ccgtttgctc tgcttcgatg
      421 cgctcgccgg cacgttccgc gagctgcgcc tgccgcgcga tccacaatgt cccggctgcg
      481 gcgcaaacgc gaagttcagc ggttaccagg acatcgaaca ggtgtgctcg gcgcatccgg
      541 gaaacgctga ataacctgct gcgctcggca tcttgcgtgc cggcagtgct cggaaccctc
      601 attcacacac aagtaaactc cggtccctgc gcgcaggcgg cacacaacct gccgtcgctc
      661 gcgacggatc ttcagcgttt ccttaactcg tcggatgctt ggtttcggcc agcgccagcg
      721 cgaaaagttg ttcctgcgtc gcttccttgc cgtatttggc cttccattcc gcgtagggct
      781 cgccgtagat gcgctcgcgc gcggcatcct tgccgatttc cacgccgcgc tcggcagcgg
      841 cctcgcggta ccagtcagca aggcagttgc ggcagaaacc ggccagtatc atcaaatcga
      901 tgttctgcac gtcgctgcgc tcgacgttga ggtgttgcaa caagcggcgg aacgccgcgg
      961 cctcgatttc gactgtgttg tttgcgttca tgatcagctc cttgtggtgc cgatattcgt
     1021 attctaacgc cgtcggcgtt tgagggatgt gcgcgtgacg gtagaatggc gcgcccttcc
     1081 agctcaggat gttccgatgg ccgcccggat tctcgacggc aaacgcgtcg ccgacgaact
     1141 gctcgacagc ttgcgcgcgc gtgttgcgca gcgcgtggca cggggcaaat cggcgccggg
     1201 gctggctgtt ctgctggtcg gcgacgacgc ggcctccgcc gtgtacgtgc gcaacaaacg
     1261 ccgcgcgtgc agacatgtgg gcttccgctc gcgtgattac gatctgccgg catcgacgac
     1321 gcaggccgaa ctcgctgcgc tgatcgacaa gctcaacgcc gacccacaga tccacggcat
     1381 tctcgtgcaa ctgccattgc cggcacgcat cgacgcgaat gcgctgatca atcgcatcga
     1441 cccacgcaag gacgtcgatg gcttccatcc ggagaacatc ggtcggctcg ccctgcgcca
     1501 gcgcggattg aggccgtgca cgtccaaggg cgtgatgacc ctgctcgcgc ataccgaccg
     1561 cccggtgcgc ggccaatccg ccgtcgtcgt cggcgtatcc aaccacgtcg gccgaccgct
     1621 ggcgttcgag ctgatgttgg cgggctgtac caccatctgc tgccacaagt tcacccgcga
     1681 tctggcgcac gaagtgggcg agggcgacat cgtgatcgtc gcggcaggca aacccggcct
     1741 ggtcaagggc gcatggatca agcccggtgc ggtggtgatc gacgtcggca tcaaccgcct
     1801 ggccgacggg tcgctcgccg gcgacgtcga attcgcgccc gccgccgaac gcgcgtcgtg
     1861 gatcacgcca gtacccggtg gcgtggggcc gatgacggtt gcgacactaa tggaaaacac
     1921 gctggaagcc gccgaacttt cctgaatcgc gctgcaatcg cgcggcgttt tcactgcgaa
     1981 ttacgggcga cttgggctat ccttcgcgcc ccatgatttc gaatcctctg cccatgcgtc
     2041 gcctgcgttt tgccgtgttg ctggccggcg tactggtcgc cgcctgcggc agcgtgatgc
     2101 gcaaggcgac cgaccagttt gccgacaacc tgaccacggc gatcctcaac gaggacgatc
     2161 ccggcaccgt gcgcgacggc gtaccggcct acctgctgct gatcgacggc atgatacagg
     2221 gcgatcccga aaacgccgga gccttgcttg caggcgccaa actctatggt gcgtatgccg
     2281 gcggattcgt cagcgatccg gcgcgtacgc aacgcatggc cggacgcgcc tacgattacg
     2341 cgcaccacgc gctatgcctg cgcgagaagg atctgtgcga ggcgctggac aagccttacg
     2401 acgcctacgt cgccgaactg gcccggaccg ggcgcggcga cgtgccggtc gtgtacggat
     2461 tcgccgccgc ctgggccggc aagatccagg tcaacagcgg tgactggaac gcgatcgccg
     2521 acctgcccaa gttgcaatcc acgctggagc gctgcacgac gctggacccg cattacgaca
     2581 atggcggcgc gtatctgtat ctgggcgtga tcaattccat ccgtccggct tcgctcggcg
     2641 gcaagcccga gcagggcaag gcctatttcg agaaggcgct ggaattgtcc ggcggcaaga
     2701 accagatggt gcgcgtgctg tacgcgcagt tctatgcgcg tctggtgttc gacaaggatt
     2761 tgcacgacaa gctgctgaac gaagtcctcg ccgccgatcc gcatgcgccg ggactgacgc
     2821 tcatcaacac gttggccaag gaaaaggcga aggccttgct tgaatccggc aaggattatt
     2881 tctgattttt aggtatatgc aaatgaataa atacacattg cttgccgcaa ttctgctcgt
     2941 gggctctgcc caggccgcca ccaccatcaa gatcgccacg gtcgccccgg acggcaccgc
     3001 ctgg
//
LOCUS       DASRVO010000145         3591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_315816, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000145 DASRVO010000000
VERSION     DASRVO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315816"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFF05_01675"
     CDS             <1..1048
                     /locus_tag="VFF05_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZW50546.1"
                     /translation="YNERIRVAGTDADDAALIDAFERIEAARKADSIESVPLTYFEFG
                     TLAALWIFAQSKLDVAVLEVGLGGRLDAVNIVDADAAIVVTVDLDHQDWLGNDIDCIG
                     REKAGIFRRDRPAIVGMRSPPKGLLDEAASIDAQLLRAGKDFEVRPQIDAWRWECADA
                     NLLLPEPELAAPCQIDNAAAAIMALYALRERLGWDAHAIARGVREARIPARLQRLHKR
                     GAPELVIDVGHNPQAARVLAQWLGEHPAHGRNIAVFGALSDKDIAGIAAPLMPHIAQW
                     HLGGLDADSPRGLSAVQLRERMPAATTLEYPGVDAALDSASAQAAADDRLLAFGSFFV
                     AAPALEWARRTGYR"
     gene            1104..2012
                     /locus_tag="VFF05_01680"
     CDS             1104..2012
                     /locus_tag="VFF05_01680"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZW50547.1"
                     /translation="MDSALKQRLIGAAVLIALAVIFVPMFLGNAPPQPQNTTLNLDIP
                     KPPDRDFQTRTLPVTGTESAVAPTANPDKIVTVDTHATPVVDAHPENSAPIAAKPVEA
                     PKPAPVHAEAPKSAPPASASVVTTPEGRFVVHLGVYADRGHADALVAALKKRGFAAYD
                     EATDYQGKTAQRVRVGPYADRAAAETARQKIRQAEPKVPSSVVELAAAAQPTADAPAS
                     ALPANRAGGWAVQLGAFKSEAEANKLRDRLRVASFAGFVERAGSGDAALWKVRAGPYA
                     DRAGADKARGDIDAKLKVKGIIVTQP"
     gene            2105..2659
                     /locus_tag="VFF05_01685"
     CDS             2105..2659
                     /locus_tag="VFF05_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZW50548.1"
                     /translation="MNWADYCILAALGLSVLMGLVRGFIGEVLALAGWVLAFWFAWQF
                     GDSVAVRLTAVEQPSIRLLLGYGLCFLLVLIASGIVNFLMRKLVAGSGLSGSDRLLGM
                     VFGLVRGLALVTITVLLLGFTPLPRDPWWHRSQLLPAFQNYAQWLSARLPPEATKYLD
                     LRGILPQIPAAPQKAADPHNHSAI"
     gene            2681..>3591
                     /locus_tag="VFF05_01690"
     CDS             2681..>3591
                     /locus_tag="VFF05_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002806604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZW50549.1"
                     /translation="MCGIIGIVGKSEVAASLYDGLTVLQHRGQDAAGIATVDGERLRL
                     HKGAGLVRDVFGTADMLKLRGRVGIGHCRYPTAGSESTAEAQPFYVNSPYGIALGHNG
                     NLVNTDALRREMFEDDRRHINTDSDSEILLNILAHELQIQERHALTPDHIFKAVAGVH
                     ARARGGYAIVSLLLGYGIVAFRDPYGIRPLVLGERDTDNGMEYAVASESVALDILGFR
                     RLRDVAPGEAVLLTQDGHLHSRHCAEGYVHAPCIFEYVYLARPDSMIEDVSVYKARLR
                     MGERLAAKIARLKPDHDIDAVIPIPDTA"
BASE COUNT          613 a         1203 c         1149 g          626 t
ORIGIN      
        1 ctacaacgag cgcatccgcg tcgccggcac ggatgccgac gatgccgcgc tgatcgacgc
       61 cttcgaacgc atcgaagcgg cgcgcaaggc cgattcaatc gagtccgttc cgcttaccta
      121 cttcgaattc ggcacgcttg ccgcattgtg gatattcgcg caaagtaaac tcgatgtggc
      181 cgtgctcgaa gtcgggctcg gcggacgcct cgatgccgtc aacatcgtcg atgccgatgc
      241 cgccatcgtc gtcaccgtgg acctggatca ccaggactgg ctgggcaacg acatcgactg
      301 catcggccgc gagaaggccg gtattttccg cagggatcgc ccagccatcg tcggcatgcg
      361 ctcgccacca aagggtttgc tcgatgaagc cgccagcatc gatgcgcagc tgctgcgggc
      421 gggaaaggat ttcgaagtcc gaccgcagat cgacgcgtgg cgctgggaat gcgctgatgc
      481 gaatttgctg ttgcccgaac ccgaacttgc cgcgccttgc cagatcgaca acgccgccgc
      541 ggcgatcatg gccctgtacg ccctgcgcga acgtctcggc tgggacgcac acgccatcgc
      601 gcgcggcgtg cgcgaggcgc ggattcccgc gcgcctgcag cggctgcata aacgcggcgc
      661 ccccgaactc gtcatcgacg tcggccacaa tccgcaggcg gcgcgcgtgc tcgcgcaatg
      721 gctcggcgag cacccggcgc atggccgcaa catcgccgta ttcggcgcgt tgtcggacaa
      781 ggacattgcc ggcatcgccg cgccgctgat gccgcacatt gcgcaatggc atctgggcgg
      841 actcgacgcc gacagtccgc gcggcctgag cgccgtacaa ctgcgcgaac gcatgcctgc
      901 ggcgacgaca ctcgaatacc ccggtgtcga tgccgcgctc gattccgcat ccgcgcaggc
      961 cgcggcggac gatcgcctcc tcgcgttcgg ttcgtttttc gtcgctgcgc cggcgctgga
     1021 atgggcgcgg cgcacgggtt atcgctgaat ctaacgggta taatcgcgcc gatgtaatca
     1081 cgccgatcgt cgaggaggcg ctcatggatt ccgcactgaa acaacgcctg atcggcgccg
     1141 ccgtgctgat cgcgctggcc gtgatcttcg tgcccatgtt cctgggcaat gcgccgccgc
     1201 agccgcagaa cacgacgctg aatctcgaca tccccaaacc gcccgaccgc gatttccaaa
     1261 cccgcaccct gccggtaacc ggcacggaat ccgccgtggc accgaccgcg aatccggaca
     1321 agatcgtgac cgtggatacg cacgccacgc ccgtggtgga tgcgcatccg gagaactccg
     1381 caccgattgc cgctaagccc gtcgaagcgc cgaagccggc gcctgtccat gccgaagcac
     1441 cgaaatctgc gccacccgct tcggcatccg tcgtgacgac gccggaaggc cgcttcgtcg
     1501 tgcatctggg cgtctacgcc gaccgcggcc acgccgacgc gctcgtcgct gcgttgaaaa
     1561 agcgcggatt cgccgcctac gatgaagcca ccgattacca gggcaagacg gcacagcgtg
     1621 tgcgtgtcgg cccctatgcg gaccgtgccg ctgcggaaac cgcgcgtcag aagatccggc
     1681 aagccgaacc gaaggtaccg agcagcgtgg tcgaactcgc cgctgcggct cagcccaccg
     1741 cggacgcgcc ggccagcgcg ttgccggcca atcgtgccgg cggttgggcc gtgcagttgg
     1801 gcgcgttcaa atccgaggcc gaggcgaaca agttgcgcga ccgtctgcgc gtggcgagct
     1861 tcgcgggatt cgtcgagcgc gccggcagcg gcgatgcggc attgtggaaa gtccgcgcgg
     1921 gtccctatgc cgaccgcgcc ggtgccgaca aggcgcgcgg cgatatcgac gcgaaactta
     1981 aagtgaaagg cataatcgtg acacagccgt gagaggcgaa ccatgatggc cgtccgtttc
     2041 agcccccttg agtcaagggg gctatccaat acttgcgcag caaaagttgg ataggggttg
     2101 tggcatgaat tgggccgact actgcattct cgctgcgctc ggcctttcgg tactgatggg
     2161 cctcgtgcgc ggtttcatcg gcgaggtgct ggcgctggcc ggatgggtgt tggcgttctg
     2221 gtttgcctgg cagttcggcg atagcgtcgc ggtgcgattg acggcggtcg agcagccgtc
     2281 gatccgcctg ttgctcggtt acggattgtg cttcctgctc gtgctgatcg cttcgggcat
     2341 cgtgaacttc ctgatgcgca agctcgtcgc cggtagcgga ttgtcgggta gcgaccgtct
     2401 gctcggcatg gtgttcggac tcgtgcgcgg gctggcgctg gtcaccatca ccgtgctgct
     2461 gctcggcttc acgccgctgc cgcgcgatcc gtggtggcat cgctcgcaac tgttgcccgc
     2521 gtttcagaat tatgcgcagt ggctgtcggc gcggttgccg ccggaggcca cgaagtacct
     2581 tgatttgcgc ggcatcctgc cgcagattcc ggccgcaccg cagaaagcgg ccgatcccca
     2641 caaccattcc gcgatctgat ccaaaaaagg cgaatcctcc atgtgcggca tcatcggcat
     2701 cgtcggaaaa tccgaagtcg cagcctcgct ctacgacggc ctgaccgtgc tgcagcaccg
     2761 tggtcaggac gccgccggca tcgccaccgt cgacggcgag cgtctgcgcc tgcacaaggg
     2821 cgcgggactg gtgcgcgacg tgttcggcac cgccgacatg ctcaagctgc gcggccgcgt
     2881 cggcatcggc cattgccgct atcccaccgc cggatcggag agcacggccg aggcgcaacc
     2941 tttctacgtc aattcgccct acggcatcgc gctcggccac aacggcaatc tggtcaacac
     3001 cgatgccctg cgccgcgaga tgttcgagga cgaccgccgc cacatcaata ccgattcgga
     3061 ttcggaaatc ctgctcaaca tcctggccca cgagttgcag atccaggaac gccacgcgtt
     3121 gacgccggat cacatcttca aggctgtcgc cggcgtgcat gcgcgcgcgc gcggcggcta
     3181 tgccatcgtc tcgctgctgc tcggctatgg catcgtcgcg ttccgcgacc cttacggcat
     3241 ccgcccgctg gtactcggcg aacgcgatac cgacaacggc atggaatacg cggtggcgtc
     3301 cgaatcggtg gcgctggata tcctcggctt ccggcgcctg cgcgatgtcg cgccgggcga
     3361 ggcggtgctg ctgacgcagg acggccacct gcacagccgg cattgcgccg aaggttatgt
     3421 gcatgcgccg tgcatcttcg aatacgtata cctggcgcgg ccggactcga tgatcgagga
     3481 cgtttccgtg tacaaggcgc gcctgcgcat gggcgagcgc ctcgccgcga agatcgcgcg
     3541 cctcaaacct gatcacgaca tcgatgcggt gattccgatt cccgacactg c
//
LOCUS       DASRVO010000146         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_160018, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000146 DASRVO010000000
VERSION     DASRVO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160018"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..808
                     /locus_tag="VFF05_01695"
     CDS             2..808
                     /locus_tag="VFF05_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZW50550.1"
                     /translation="MGGWLSGSLALLADAGHMLVDALALLLAWGGAYMARRPADARRS
                     FGYARMEVLAGYTNALTQFALAAWIVYEVALRLAHPLPIRSGLMLVVAVAGLAVNALV
                     LRVIGTHAHDDVNAAGARLHVLGDLLGSLAAVAAALLIRYFGWLVADPALSVVVALLI
                     VVSAWNLLRRSAHILLEGTPDDMQAVDVAGAIERETGIGGVHHLHVWQLAGGRRVATL
                     HAQVSAQADAHTALTAIQRVLRERFGIAHATVQIEQTECATADCDGDRGH"
     gene            885..1067
                     /locus_tag="VFF05_01700"
     CDS             885..1067
                     /locus_tag="VFF05_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HZW50551.1"
                     /translation="MGRGDRKTRKGKIAIRSFGNARPRKPAAKAGGAVKKAAPAKAAV
                     KKAAPAVKKAAPKKSA"
     gene            complement(1117..2352)
                     /gene="hutI"
                     /locus_tag="VFF05_01705"
     CDS             complement(1117..2352)
                     /gene="hutI"
                     /locus_tag="VFF05_01705"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZW50552.1"
                     /translation="MTTSASPRWDHLLLECRLATMADNGVAYGAIENAALGWKDGVIA
                     FAGPQSGLPGKPTDLAAQVGSADDAWITPGLIDCHTHLVFAGNRAEEFELRLQGASYE
                     EIARRGGGIVSTVNKTRAASEDELFAQSLPRARALMRDGATTIEIKSGYGLNLDSEAK
                     MLRVARRIGAELRITVHTTFLGAHALPPEFAGRQADYVDEVCVRMLPAIARLGLADAV
                     DAFCEGIAFAPSEVRRVFEEARALGLRVKLHADQLSDLDGAALAAEFGALSADHLEYA
                     NEAGVRAMAASGSAAVMLPAAFYALRETQLPPIASFREHGVPLAVASDVNPGTSPILS
                     LRMAMNMTCALFRMTPEEALRGATVNAARALGLSDRGALETGKRADFVVWDVDHPAEL
                     SYWIGGSLARRVVAGGVDV"
     gene            complement(2362..>2656)
                     /locus_tag="VFF05_01710"
     CDS             complement(2362..>2656)
                     /locus_tag="VFF05_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZW50553.1"
                     /translation="TDQGAVILLGMAAFLTGVTQAPITSAVISLELTANQNMVIPIMA
                     VCLLARGVSALVCHTPVYRAFAIRAINEFEKEAAASVSPPTPPAPPQIPPPVN"
BASE COUNT          434 a          865 c          909 g          448 t
ORIGIN      
        1 cattggcggt tggctgtccg gatcgcttgc cctgctcgcc gatgccggcc acatgctggt
       61 cgacgcgctg gcgttgctgc tggcctgggg tggggcgtac atggcgcgac ggccggccga
      121 cgcgcggcgc agcttcggct acgcgcgcat ggaggtgctg gccggataca ccaacgcatt
      181 gacgcaattc gctctcgccg catggatcgt gtacgaggtg gcattgagac ttgcccatcc
      241 gctaccgatt cgctccggcc tgatgctcgt tgtcgcagtg gcgggactgg ccgtcaacgc
      301 actcgtattg cgggtgatcg gcacgcatgc gcacgacgac gtcaacgctg ccggcgcccg
      361 cctacacgtg ttgggcgacc tgctcggatc gcttgcggcc gtggccgccg cgctgctgat
      421 ccgttatttc ggctggctcg tggccgatcc ggcgctgtcg gtcgtggtgg cattgctgat
      481 agtcgtcagc gcctggaacc tgctgcgccg tagtgcgcac atcctgctcg aaggcacgcc
      541 ggacgacatg caggctgtcg acgtggccgg ggcgatcgag cgggaaaccg gcatcggcgg
      601 cgtgcatcat ttgcatgtgt ggcaactcgc cggcggtcga cgcgtcgcca ccttgcacgc
      661 gcaggtgtcg gcgcaggccg atgcgcacac ggcgttgacg gcgatccagc gcgtgctgcg
      721 cgagcgtttc ggcattgcgc acgcgaccgt gcagatcgaa caaaccgaat gcgccaccgc
      781 cgactgcgac ggcgatcgcg ggcactgatt ttccttgctt gcgcgccgcg tgctggtaag
      841 ctacgcaccc gattcatacc acaaaatact ggagcacgat tccgatgggc agaggcgacc
      901 gcaagacacg caagggcaag atcgcgatcc gcagtttcgg caatgcgcgt ccgcgcaagc
      961 cggcggcgaa ggcgggtggc gccgtgaaga aggccgcgcc ggccaaggcc gccgtgaaga
     1021 aggccgcccc ggccgtgaag aaggcggcgc cgaagaaatc agcctgagtc tcgtttcagg
     1081 ttccacgaag ccccgccgtg cgcggggctt tgtctttcag acgtccacac cgcccgcgac
     1141 gacgcgccgg gcgagactgc cgccgatcca gtagctcaat tcggcgggat gatcgacatc
     1201 ccagacgacg aagtcggcgc gcttgccggt ttccagcgcg ccacggtcgg atagccccag
     1261 cgcgcgcgcg gcgttgacgg tcgcaccgcg cagggcttct tccggcgtca tgcggaacag
     1321 ggcgcaggtc atgttcatcg ccatgcgtaa cgacaggatc ggcgaggttc cgggattgac
     1381 gtcgctggcc accgccagcg gcacgccgtg ctcgcggaat gaagcgatcg gcggaagctg
     1441 cgtttcgcgc agggcataaa acgcagctgg cagcatcact gcggcgctgc cggaagcagc
     1501 catcgcgcgc acgccggctt cgttcgcgta ttccaggtga tcggcggaga gcgcgccgaa
     1561 ctccgcggcc agcgccgcgc cgtccagatc cgataactga tcggcatgca gcttcacacg
     1621 caggccgagc gcgcgcgctt cctcgaaaac gcggcgcact tccgacggcg cgaacgcaat
     1681 accttcgcag aatgcgtcta cggcatcggc cagtcccagg cgcgcgatgg caggcagcat
     1741 gcgcacgcag acctcgtcca cgtaatcggc ctggcgcccg gcgaattccg gcggcagcgc
     1801 gtgcgcacca aggaacgtgg tgtgcacggt gatgcgcagt tccgccccga tgcgccgcgc
     1861 cacgcgcagc attttggcct cggaatccag attcaatccg tagccggatt tgatttcgat
     1921 ggtcgtcgcg ccgtcgcgca taagcgcacg cgcgcgcggc agcgattgtg cgaacaactc
     1981 gtcttcgctg gcggcacgcg ttttgttcac ggtggagaca atgccgccgc cgcggcgggc
     2041 gatttcttcg taactcgcgc cttgcagtcg cagctcgaat tcctcggcgc gattgccggc
     2101 gaagaccaga tgcgtgtggc agtcgatcag cccgggcgtg atccaggcgt cgtccgccga
     2161 tccgacctgc gcggcgaggt ccgtcggctt tccgggcaat ccggattgcg ggcccgcaaa
     2221 tgcgatgacg ccgtccttcc agcccagcgc ggcgttttcg atggcgccat aggcaacacc
     2281 gttgtccgcc atcgtggcga ggcggcactc gagcagcaga tggtcccagc gcggcgacgc
     2341 ggatgtggtc atcggacgcc ctcagttcac cggcggtgga atttgcggtg gtgcgggggg
     2401 cgtcggtggc gagacgcttg ccgcggcttc cttctcgaac tcgttgattg cgcggatcgc
     2461 gaatgcgcga tacacgggtg tgtggcacac cagcgcggaa acaccgcggg caagcaggca
     2521 caccgccatg atcgggatca ccatgttctg gttggccgtg agttcgagcg agatcacggc
     2581 cgaggtgatt ggcgcctgcg tcacgccggt caggaacgcg gccatgccaa gcaggatgac
     2641 cgcaccctgg tccgtg
//
LOCUS       DASRVO010000147         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_30397, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000147 DASRVO010000000
VERSION     DASRVO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30397"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VFF05_01715"
     CDS             <1..571
                     /locus_tag="VFF05_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZW50554.1"
                     /translation="VADATFVQFQQGTPINDTVLKAEYDNHAARAGKVAYDFSQLLFD
                     DDDDALKAEDDIVSGKPFDKVFDAWRAKAKQARRFTRVLPDQLPPELAKALADLRNGD
                     STKTPVKTEYGWHVVHLDIANPYTPPPFDQVKDGIRRNLQMKIGRERLDKLREQAKIE
                     YAPNVKPPVKAAETAPVAPAPSAMPAAKN"
     gene            complement(568..957)
                     /gene="queF"
                     /locus_tag="VFF05_01720"
     CDS             complement(568..957)
                     /gene="queF"
                     /locus_tag="VFF05_01720"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZW50555.1"
                     /translation="MSTAPSKTLETFANPEPGRDYTIRMRIPEFTCLCPKTGQPDFAT
                     LHLEYVPDKTCVELKSLKLYVWSFRNEGAFHEAVTNRILDDLVKATQPRFMRLSADFG
                     VRGGIYTNVVAEHRAPAWQAPAKVELP"
     gene            1070..>2120
                     /locus_tag="VFF05_01725"
     CDS             1070..>2120
                     /locus_tag="VFF05_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZW50556.1"
                     /translation="MRLTTIKLSGFKSFVDPTVLHLPTNMTGVVGPNGCGKSNIIDAI
                     RWVMGESAPTRLRGDSLSDVIFSGSTGRKPVGTASVELIFDNSDGLVVGEYAKYNEIS
                     VRRQASRDGQSQYFLNGARCRRRDITDLFLGTGLGARSYSIIEQGMISEIISAHPDEL
                     RGHLEEAAGISKYKERRKETESRIKATRENLERLNDVREEVDKQLDHLNRQAKAAARW
                     QELKAEHGRRESELRGVHYRAIAAEIDEHHHALAQADIAIEGFVAEQRQVEVQIEAHR
                     EKHQAATDHLNQVQGEVYRIGGEIARVEQQIQHSKELHERLERAREETEKARAELGEH
                     IGGDQQQIELLRAALA"
BASE COUNT          423 a          664 c          692 g          341 t
ORIGIN      
        1 cgtggcggat gcgacattcg tccagttcca gcagggcacg ccgatcaacg acaccgtgct
       61 caaggccgag tacgacaatc atgctgcgcg tgccggcaag gtcgcctacg acttcagcca
      121 gctgctgttc gacgacgatg acgatgcact caaggccgag gacgacatcg tttcgggcaa
      181 gcctttcgac aaggttttcg atgcgtggcg cgccaaggcg aagcaggcgc gccgtttcac
      241 gcgcgtgctt cccgatcagt tgccgccgga actggccaag gcgctcgcgg atttgcgcaa
      301 tggcgattcc acgaaaacgc cggtgaagac cgagtacggc tggcatgtcg tgcacttgga
      361 catcgccaat ccctacacgc cgccgccgtt cgatcaggtc aaggacggca tccgccgtaa
      421 cctgcagatg aagatcgggc gcgagcgcct ggacaagctg cgcgagcagg caaagatcga
      481 atatgcgccg aacgtgaagc cgccggtgaa agctgcggag accgcgccgg tggccccggc
      541 gccctcggcc atgcccgctg caaagaacta gggcagttcg accttggcgg gcgcttgcca
      601 cgccggcgcg cgatgctcgg cgactacgtt ggtgtagatg ccgccgcgca cgccgaagtc
      661 ggcggacagg cgcatgaagc gcggctgcgt ggccttgacc aggtcgtcga ggatgcgatt
      721 ggtcacggct tcatggaagg cgccttcgtt gcggaagctc cagacgtaaa gcttgagcga
      781 cttgagctcc acgcaggtct tgtccggcac gtattccaga tgcagggtgg cgaagtccgg
      841 ctgcccggtt ttcgggcaca ggcaggtgaa ctcgggaatg cgcatgcgga tggtataatc
      901 gcggcccggt tccgggttcg cgaacgtttc cagggttttg ctgggcgcgg tggacacggc
      961 actcactcct gctgcggctt tcttgcgtgg gcgcgcacct tagcgcaagc ccgggcgctg
     1021 gacaatgcgg cgtcgccagt ttctgaatgg aatccctcga agcacaccca tgcgtctgac
     1081 cacgatcaag ctttccggat tcaaatcctt cgtcgatccg accgtcctgc acctgccgac
     1141 caacatgacc ggcgtggtcg gtccgaatgg ctgcggcaag tccaacatca tcgatgcgat
     1201 tcgctgggtg atgggcgaga gcgcgccgac gcgtctgcgt ggcgattcgc tcagcgacgt
     1261 gatcttttcc ggttcgaccg gacgcaagcc ggtcggtacg gcttcggtgg aactgatctt
     1321 cgacaactcc gacggcctcg tcgtcggcga gtacgcgaag tacaacgaaa tttcggtcag
     1381 gcgccaggcc agccgcgacg gccagtcgca gtatttcctc aacggcgcgc gctgccgccg
     1441 ccgcgacatc accgatcttt tcctcggcac gggtttgggc gcgcgcagct attcgatcat
     1501 cgagcagggc atgatctccg agatcatctc cgcgcatccg gatgaattgc gtggacacct
     1561 cgaagaggcc gccgggattt ccaagtacaa ggagcggcgc aaggaaaccg agagccgcat
     1621 caaggccacg cgcgagaacc tcgaacgcct gaacgacgtg cgcgaggaag tcgacaagca
     1681 actcgatcac ctcaaccgcc aggccaaggc cgccgcgcgc tggcaggagc tgaaagccga
     1741 acatggtcgg cgcgaatcgg aactgcgcgg cgtgcactat cgcgccatcg ccgccgagat
     1801 cgacgagcac catcacgcgc tggcgcaggc cgacatcgcg atcgaaggct tcgtcgcgga
     1861 gcagcgccag gtcgaagtgc agatcgaagc gcatcgcgaa aagcaccagg ccgccacgga
     1921 tcacctgaac caggtgcagg gcgaggtcta ccgtatcggc ggggaaatcg cccgcgttga
     1981 gcagcagatc cagcacagca aggaactgca cgagcgcctc gagcgcgcgc gcgaggaaac
     2041 cgaaaaggcg cgcgcggaac tcggcgagca catcggcggc gaccagcagc agatcgaatt
     2101 gttgcgcgcg gcgctggccg
//
LOCUS       DASRVO010000148         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_186414, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000148 DASRVO010000000
VERSION     DASRVO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186414"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFF05_01730"
     CDS             complement(<1..534)
                     /locus_tag="VFF05_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016901647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZW50557.1"
                     /translation="MPIAAKFEIEYLQYMDAEGKLVREDLPEFARDMKQMVELYKLMV
                     STRVFDTKSIALQRTGKLGTYASCLGHEATHVGIGSSLKPEDVLAPSYREYGAQLYRG
                     VRPRDVYMYWGGDERGNDYPNEPAKHDFAWSVPIGTQCLHAAGSALAFKIRGEKRVAV
                     FTIGDGGSSKSDFYGAIN"
     gene            722..>1032
                     /locus_tag="VFF05_01735"
     CDS             722..>1032
                     /locus_tag="VFF05_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HZW50558.1"
                     /translation="MTKPNERELEAGIQTDLRDRLTYGGYLQLDTLLAAQKPLSRPAH
                     HDEMLFIIQHQTSELWLKLIVHELRAALEHLRGDDVNPCLKILARVKQIQRQLFEQWA
                     VL"
BASE COUNT          200 a          315 c          325 g          192 t
ORIGIN      
        1 gttgatcgcg ccgtagaaat cggatttcga ggaaccgcca tcgccgatcg tgaatacggc
       61 gacgcgcttt tcgccgcgga tcttgaaggc gagggcggaa ccggcggcat gcaggcactg
      121 cgtgccgatc ggcaccgacc aggcgaagtc gtgcttggcc ggttcgttcg ggtaatcgtt
      181 gccgcgttcg tcgccgcccc agtacatgta gacgtcgcgc ggacgcacgc cgcgatagag
      241 ctgcgcgccg tattcgcgat agctcggcgc gagcacgtcc tcgggcttga gcgaactgcc
      301 gatgccgacg tgcgtcgctt cgtggccgag acacgatgca taagtgccga gtttgccggt
      361 acgctgcagc gcgatcgact tggtgtcgaa cacgcgggtc gacaccatca acttgtacag
      421 ctcgaccatt tgtttcatgt cgcgggcgaa ctcgggcaag tcctcgcgca cgagcttgcc
      481 ttcggcatcc atgtattgca ggtattcgat ttcgaacttg gcggcgatgg gcacgacggg
      541 attcctcggg acagcgtgta agttaagcgt gccgtggcgg cgatggcaag gttttgcgca
      601 ataaattacg caataaaacg gggctcgacg gattcaaacc ccgtttgatg caagtcaatg
      661 gcgatgcctc gcgccggcgc gtaccattga ccgttcctgt cgcccagcag ttccgagacc
      721 gatgaccaag cccaacgaac gcgaactcga agccggcatc cagaccgatc tgcgcgaccg
      781 gctgacctac ggcggctacc tgcaactcga tacgctgctc gcggcgcaga agccgctgtc
      841 caggccggcg caccacgacg agatgctgtt catcatccag caccagacct cggagttgtg
      901 gctcaagctg atcgtgcacg aactgcgcgc cgcgctcgaa cacctgcgcg gcgatgacgt
      961 gaacccgtgc ctgaaaatcc tggcgcgggt gaagcaaatc cagcgccagc tgttcgagca
     1021 gtgggcggtg ct
//
LOCUS       DASRVO010000149         1238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_186534, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000149 DASRVO010000000
VERSION     DASRVO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186534"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFF05_01740"
     CDS             complement(<1..276)
                     /locus_tag="VFF05_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50559.1"
                     /translation="MCHALFSRAGRFLACPDLRPRRMDIREHSVALISVVIGLGLTSL
                     LGNFNRLMRRRGEIRWDALPLAWALIALMLVNNYWWGVYLGMVTSTAA"
     gene            495..1238
                     /locus_tag="VFF05_01745"
                     /pseudo
     CDS             495..1238
                     /locus_tag="VFF05_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
BASE COUNT          225 a          397 c          424 g          192 t
ORIGIN      
        1 ggcggccgtc gacgtgacca tgccaaggta aaccccccac cagtagttgt tgaccagcat
       61 cagggcaatc aacgcccagg ccagcggtag cgcatcccag cggatctcgc cgcgcctgcg
      121 catcagccgg ttgaaattgc cgagcaggct ggtcagaccc aggccaatga cgaccgagat
      181 cagggcaacg gagtgttcgc ggatatccat gcggcgaggc ctcaagtccg ggcaggcaag
      241 gaatcgtccg gcgcgcgaga atagcgcatg acacatcgat ggcgccaaag ttcgggtcac
      301 tacagccagc atcgggcggc agtcgagcgc cgtcgatgtt cgcgccttgc accgcgtgca
      361 tgcggtcgga atcgccgttg cggcagcgtt tcacgcgccg aacggatacg atggtcgcgg
      421 gtaccgatgc gccgtgaaac ggcccgtgcc gtcagacaag gactgatgcg atgaatgagg
      481 cgaagaacga ttacctgcaa cgctggctgc tcgggcttgc gcgcccgctt ggcgcgatcc
      541 gcatggcagt ggtgcatccg tgcgacgcga ccagcctggc cgcggcgctg gatgcgcgtg
      601 ctatcggcat cgtcgaaccg gtgctggtcg ctccgcgcgc caagctcgaa cgcattgcgc
      661 gggagaacaa gctcgacctc gccggcatcg cgatcgagga tgccgcgcat agccatgccg
      721 ccgcggcaca ggcggtcgcg ctcgcgcgcg cgggcagggt cgaggcgctg atgaagggca
      781 gcttgcacac cgacgagatc atgggcgccg tggtcgcggc tgatagcggc ctgcgcaccg
      841 cgcggcgcat ctcgcattgt tttttgatgt acacgccggc gtatccgcgt ccgttcatca
      901 tcaccgacgc ggcggtgaac atcgcgccga cgctggacga gaaggccgac atcgtgcgca
      961 acgcgatcgc gctggcgcaa gtcatcggcg tgcagacgcc gcgcgtggcg attcttgccg
     1021 cggtggaaac ggtcaattcg cgcatgcagg caacgcttga cgccgccgct ttgtgcaaga
     1081 tggccgaccg cggacagatc gccggcggcg tgctcgacgg accgctcgcg ttcgacaatg
     1141 cggtgtcggc ggaatccgcg cgcatcaagg gcatcaactc cgatgtcgcc ggacgcgccg
     1201 acgtgctggt cgtgccggac ctggaatccg gcaacatg
//
LOCUS       DASRVO010000150         1399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_186546, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000150 DASRVO010000000
VERSION     DASRVO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186546"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFF05_01750"
     CDS             <1..223
                     /locus_tag="VFF05_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50560.1"
                     /translation="YSAGRIWSRPALIRVGSVCWVAAFALALAIAVLTPLDFGWKLLI
                     LASAVVYAIIPPMTWRYLERLHHEQEPQR"
     gene            220..552
                     /locus_tag="VFF05_01755"
     CDS             220..552
                     /locus_tag="VFF05_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50561.1"
                     /translation="MIANLINTVTGLVLAYATVLHPTWVEQRYLPFAGFAVVILAMAL
                     WARRSDPHPWFSSVNIVLAITLGILSLLPLATLPNLTFWGGFWVGALVPTVALWAALY
                     RPQPAAAR"
     gene            588..977
                     /locus_tag="VFF05_01760"
     CDS             588..977
                     /locus_tag="VFF05_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZW50562.1"
                     /translation="MTKPAVIAGIALAAALAASATHAADPAAGKAKYETTCVACHGAN
                     GISIAPIYPNVAGQKEEYLIAQLKAFRDGSRQNPIMAPMAKGLTDTDIASISLYIFTL
                     KPWRRRRPLCLAAAYNERSLVCFRRTW"
     gene            974..1297
                     /gene="fdxA"
                     /locus_tag="VFF05_01765"
     CDS             974..1297
                     /gene="fdxA"
                     /locus_tag="VFF05_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HZW50563.1"
                     /translation="MTHVVTDNCIKCKYTDCVEVCPVDCFHEGPNFLAIDPEECIDCT
                     LCVPECPAEAIFPEDDVPAGQENFLRINAELAPLWPVIAARIDAPADADDWNGTPDKL
                     PMLER"
BASE COUNT          244 a          464 c          427 g          264 t
ORIGIN      
        1 ctacagcgcc ggccggatct ggtcgcgccc ggcgctgatc cgggtcggca gcgtgtgctg
       61 ggttgcggcg ttcgcgctgg cgctggcgat tgcagttcta acgccactcg atttcggctg
      121 gaaactgttg attctggcca gcgccgtcgt ctacgcgatc atcccgccga tgacgtggcg
      181 ctacctcgaa cgcttgcacc acgaacagga accgcagcga tgatcgcgaa cctcatcaac
      241 acagtgaccg ggctggtgct ggcatatgcc accgtgttgc atccgacctg ggtcgagcag
      301 cgctatctgc ctttcgcggg attcgccgtc gtgatcctgg cgatggcgct gtgggcgcgc
      361 cgcagcgatc cgcatccgtg gttcagcagc gtcaacatcg tgctcgcgat cacgctcggg
      421 atcctttccc tgctgccgtt ggcgacgttg ccgaatctca ccttctgggg cggattctgg
      481 gtcggcgcgc tggtgccgac cgtggccctg tgggccgcgt tgtaccggcc gcagcccgct
      541 gccgcccgct gaccgtacgc accggaacct gcacaggagc acacgccatg accaagcccg
      601 ccgtcattgc cggcatcgcg ctcgcggccg cacttgccgc cagcgccacg cacgcagccg
      661 atccggccgc gggcaaggcg aaatacgaga cgacctgcgt cgcctgccat ggtgcgaacg
      721 gcatctcgat cgcgccgatt tatccgaacg tcgctggcca gaaggaagaa tatctgatcg
      781 cgcaactcaa ggcgttccgc gacggcagcc gccagaatcc catcatggcg ccgatggcca
      841 agggcttgac cgacaccgac atcgccagca tttcgcttta tattttcacg ctcaagccat
      901 ggcgtcgccg ccggccgctt tgtctggccg cggcgtacaa tgagcgctcg ctggtttgtt
      961 ttaggagaac gtggtgacgc acgttgtcac cgataattgc atcaagtgca agtacaccga
     1021 ttgtgtcgaa gtgtgtccgg tcgactgctt ccacgagggc cccaacttcc tcgcgatcga
     1081 tccggaagag tgcatcgatt gcacgttgtg cgtgcccgag tgtcctgccg aggcgatctt
     1141 tcccgaagac gatgtaccgg cgggtcagga aaacttcctt cggatcaatg ccgaattggc
     1201 gccattgtgg ccggtgatcg ccgcgcgcat cgatgcgccc gccgacgccg acgactggaa
     1261 cggaacgccg gacaaactgc cgatgctcga acgctgaatg ccatgaccga cgattcctcc
     1321 agccgcgttc cgcacagcgg cgagaaattc accacgcccg aaggcatccg cgcagtctac
     1381 ggtggcatcc gcccgaatg
//
LOCUS       DASRVO010000151         1826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_290271, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000151 DASRVO010000000
VERSION     DASRVO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290271"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="VFF05_01770"
     CDS             <1..1553
                     /locus_tag="VFF05_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HZW50564.1"
                     /translation="ARTLADIVKDADIFLGLSAAGVLSGEMVKTMAARPIILALANPT
                     PEILPELARAARPDAIIATGRSDYPNQVNNALCFPYIFRGALDVGATTINEAMKVACV
                     RAIAELARREISDVAARAYGDQLLAFGPDYLIPQPFDPRLLVSLAPAVAKAAMESGVA
                     TRPIADLNAYRERLSQFVYRTGMVMKPVFERAKADVKRVVYAEGEEDTILRAVQTVVD
                     EGMAKPILIGRPAVIEKRIERLGLRIRAGEHFEMTNIDDDPRYNDYWGLYLKLTERRG
                     ITPPLAKALVRSRPSLIAALMVARGEADAMICGIVGRYQRQLRHIMDVLPLDPGVSAP
                     AAMTAVQNDRGTWFFVDTHVQYEPSAEQIAEATLQAVLRLKLFGIAPRVALLSHSNFG
                     SHDNASARKMRQVLELVRSKAPNLEIDGEMQADTALNEDIRTRIFPHSLLRGRANVFV
                     FPNLDAANIAYNMVRQFTEGVAIGPILMGVSKPAHVLTPASTVRRVVNMTSIACVEAQ
                     IRAQATPA"
     gene            complement(1560..>1826)
                     /locus_tag="VFF05_01775"
     CDS             complement(1560..>1826)
                     /locus_tag="VFF05_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50565.1"
                     /translation="YVGDLTAVIPACFDVLRPGGALIFSCEATVEAEGALVLRPSKRY
                     AHSRASIEALCRTAGFAACAIEAIDVRLENNAPIPGFIAIAEKA"
BASE COUNT          314 a          619 c          597 g          296 t
ORIGIN      
        1 tggcacgtac gttggccgac atcgtcaagg acgcggacat cttcctgggg ctttccgccg
       61 ccggcgtgct cagcggcgag atggtgaaga ccatggcggc acggccgatc atcctcgcgc
      121 tcgccaatcc cacgcccgag attcttccgg aactcgcgcg cgcggcgcgg cccgacgcga
      181 tcatcgccac tgggcgctcg gattacccga accaggtcaa caacgcgctg tgctttccgt
      241 acatcttccg cggcgcgctg gatgtcggcg cgaccacgat caacgaggcg atgaaggtcg
      301 cctgcgtgcg cgcgatcgcg gaactggcgc ggcgcgagat ttccgacgtc gccgcgcgcg
      361 cctacggcga ccagttgctg gcattcggcc cggattacct gattccgcag ccgttcgatc
      421 cgcgcctgct ggtttcgctc gcgcctgccg tggccaaggc ggccatggag tcgggcgtgg
      481 cgacgcggcc gatcgcggac ttgaacgcgt accgcgagcg cctgtcgcag ttcgtctacc
      541 gcaccggcat ggtgatgaag ccggtgttcg agcgtgccaa ggccgacgtc aagcgcgtgg
      601 tttacgccga gggcgaggaa gacaccatcc tgcgcgcggt gcagaccgtg gtcgacgagg
      661 gcatggccaa gcccatcctg atcggtcgtc ctgcggtgat cgagaagcgt atcgagcggc
      721 tcggcctgcg catccgcgcg ggcgagcatt tcgaaatgac caatatcgac gacgatccgc
      781 gctacaacga ttactggggc ctgtacctga agctgaccga acgccgcggc atcacgccgc
      841 cgctggccaa ggcactggtg cgttcgcggc cctcgctgat cgccgcgctg atggttgcgc
      901 gcggcgaagc ggacgcgatg atctgcggca tcgtcggccg ctaccagcgc cagttgcgcc
      961 acatcatgga cgtgctgccg ctcgatcccg gcgtgtccgc gccggcggcg atgaccgcag
     1021 tgcagaacga ccgcggcacc tggttcttcg tcgacacgca cgtgcaatac gagccttccg
     1081 ccgagcagat cgccgaggcc acgctgcagg ccgtgttgcg cctgaaactg ttcggcatcg
     1141 cgccgcgcgt ggcgttgctg tcgcattcga atttcggcag ccacgacaat gccagcgcgc
     1201 gcaagatgcg ccaggtgctg gagctggtgc gcagcaaggc gccaaacctg gaaatcgacg
     1261 gcgaaatgca ggccgacacc gcgctgaacg aggacatccg cacgcgcatc ttcccgcact
     1321 cactgctgcg cggccgcgcc aatgtgttcg tgttcccgaa cctggacgcc gccaacatcg
     1381 cctacaacat ggtgcggcag ttcaccgaag gcgtcgccat cggaccgatc ctgatgggcg
     1441 tgtccaaacc cgcgcatgta ctcacgccgg cctcgaccgt gcgccgcgtc gtcaacatga
     1501 cctcgatcgc ctgtgtcgaa gcgcagatcc gggcgcaggc aacgccagcc tgatgcaagt
     1561 caggcttttt cggcgatcgc gataaaacca ggaatcggcg cgttgttttc gagccgcacg
     1621 tcgatcgcct cgatggcgca agcggcgaat ccggccgtcc ggcacagcgc ctcgatcgac
     1681 gcacgcgagt gcgcatagcg tttgctcgga cgcagcacga gcgcaccttc cgcttcgacc
     1741 gtcgcttcgc acgagaagat cagggcgccg ccggggcgca ggacgtcgaa gcaggccggg
     1801 atcacggccg tgagatcgcc gacgta
//
LOCUS       DASRVO010000152         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_316190, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000152 DASRVO010000000
VERSION     DASRVO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316190"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3002)
                     /locus_tag="VFF05_01780"
     CDS             complement(<1..>3002)
                     /locus_tag="VFF05_01780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW50566.1"
                     /translation="LCGPPQLKPNGNPPPPSPFGELSESLATALAQASDHAEHPGLDR
                     KPVILHILHSWGGGSERFVRDLAAADRSRHHLALTAYGNFARRRYGEVLRLRDATWRH
                     LPLREAVLSAPIASTALTHRGYREFLDGILRDFNVDAVVVSSLIGHSLDALRTGLPTM
                     YFIHDFYPLWPLLHRNLDDASVTFDAAQLRADLAAADTGFEFAERDPEWWLTLRERMA
                     DATLAAQATLIAPSSSALAIFLKLQPHLAALAQSVIPHGIAPWPDATKLPLPAPPKRE
                     KLRLLILGRVRGGKAANLLRRLLPHLRDTELFLIGAGSEANEFFGVPGVHILLNYETA
                     ELPALLARVTPDAALMLANLPETFSYTLSELRSLGIPVIATRLGALAERITDGVDGFL
                     VAPEVTAIANAIDSLRADPVRLERMRAALARQPLRNITDMVADYSVLLPQAPRQRGTP
                     AIAETGLARIDAQVDAAVAGAASREIAALEKTLAHQRHELERRAQWAFGLDQQIDRQR
                     AEAATLRELIDARTQWAQTAMAETERARTDLATLQGEFDERSAWALRLDVEVREVREE
                     RDRIVGSLSWRITKPLRFARRKLRGLRVRLGFAASRLRAIMHRTRGSLASRGVTGTLR
                     RAADEFRRSAPPPNARIVAAPTSEFSPFDVPADTTPRVSIVIPVHNKIDYTVACLRSL
                     AVHPGTAAFEVIVVDDASSDSTAERLAQIRGIRVLRNAQNLGFVGSCNAGAAAAHGEF
                     LAFLNNDTVVEDGWLDALLHVFDEESEAGLAGAKLVYPDGRLQEAGGIVFADGSGWNY
                     GRLENPADPRFEFRREADYCSGAAIMIRRDLFEQLGGFDTRYAPAYYEDTDLSFAVRK
                     AGKKVLYEPRATVVHFEGITAGTDTGSGMKRFQAVNREKFLDKWKNELVRQPAPIHDA
                     KDAEAAANFRAHGHVLIVDAYTPTPDQDSGSLRMLNLMRLLREGGHAVSFLPDNRAHA
                     GKYTQALQALGVQALYHPFVADPVAWLR"
BASE COUNT          516 a          949 c         1027 g          510 t
ORIGIN      
        1 gcgcaaccag gcgaccggat cggcgacgaa cgggtgatac agcgcctgca cgccgagcgc
       61 ctgcagcgcc tgcgtgtatt tgccggcgtg cgcgcggttg tcgggcagga agctcaccgc
      121 atgaccgcct tcgcgcagca ggcgcatcag gttgagcatg cgcaacgagc cggaatcctg
      181 gtccggcgtc ggcgtgtagg catccacgat cagcacgtgg ccatgcgcgc ggaaattcgc
      241 cgcggcctcg gcatccttcg cgtcatggat tggcgccggc tggcgcacga gttcgttttt
      301 ccacttatcc aggaatttct cgcgattgac cgcctggaag cgcttcatgc cgctgccggt
      361 gtcggtgccg gctgtgatgc cctcgaagtg cacgaccgtc gcgcgcggct cgtagagcac
      421 tttcttgccc gccttgcgca cggcaaatga gagatcggta tcctcgtagt aggccggcgc
      481 gtaacgcgtg tcgaaaccgc cgagctgctc gaatagatcg cgccggatca tgatcgccgc
      541 gccggaacaa tagtcggcct cgcggcggaa ctcgaagcgc ggatcggcgg ggttttccaa
      601 gcgtccgtaa ttccagcccg agccgtcggc gaacacgatg ccgccggctt cctgcaagcg
      661 gccgtcggga tacacgagtt tggcaccggc caatccggcc tcggattctt cgtcgaaaac
      721 gtgcaacagc gcgtcgagcc agccgtcttc gaccacggtg tcgttgttga ggaaagcgag
      781 aaattcgccg tgcgcggcgg cggcgccggc attgcacgag ccgacgaagc cgaggttctg
      841 cgcattgcgc aggacgcgga tgccgcgaat ttgcgcgaga cgctcggcgg ttgagtcgct
      901 tgacgcgtcg tcgaccacga tgacctcgaa cgccgccgtg ccgggatgca cggcaagcga
      961 acgcaggcag gccacggtgt agtcgatctt gttgtggacc ggaatcacga tcgatacgcg
     1021 cggcgtcgtg tccgccggca cgtcgaacgg actgaactcg ctcgtcggcg cggccacgat
     1081 gcgtgcgttt ggcggcggcg cactgcggcg gaattcgtcg gcggcgcggc gcagcgtacc
     1141 ggtcacgcct cggctggcga ggctgccgcg cgtgcggtgc atgatcgcgc gcaggcggct
     1201 tgcggcgaaa cccaagcgca cgcgcaaccc gcgcagcttg cgccgggcga agcgcaaggg
     1261 cttggtgatg cgccaagaca gactgccgac gatgcgatcg cgttcctcgc gcacttcacg
     1321 cacttccacg tcgaggcgca acgcccatgc gctgcgctcg tcgaactcgc cctgcaatgt
     1381 ggcaagatcg gtgcgcgctc gctcggtctc cgccatcgcg gtctgggccc actgtgttcg
     1441 tgcatcgatc aactcccgca gcgtcgctgc ctcggcgcgc tgacgatcga tttgctgatc
     1501 cagaccgaag gcccactgcg cacgtcgttc gagttcgtgg cgctggtgcg ccaaggtttt
     1561 ctccagcgcg gcgatctccc ggcttgcggc gccggccacg gcggcgtcca cttgcgcatc
     1621 gatgcgggca agtccggttt cggcaattgc aggagttccg cgctgtcgcg gagcttgcgg
     1681 cagcagcacg ctgtagtccg cgaccatgtc ggttatgttg cgcagcggtt gacgtgcgag
     1741 tgcggcacgc atgcgctcga gtcggacagg gtccgcacgc agcgaatcga tggcgttagc
     1801 aatggcggtg acctccggcg cgacgagaaa tccatccacg ccatcggtga tgcgttcggc
     1861 gagcgcgccc agacgcgtgg cgatgacggg gatgcccaga ctgcgcagct cggaaagggt
     1921 gtagctgaac gtttcgggca ggtttgccag catcaaggcc gcatccggcg tgacgcgtgc
     1981 gagcagcgca ggcaattccg cggtctcgta attgagcagg atgtgcacgc cgggcacgcc
     2041 gaagaattcg ttcgcctcgg atccggcgcc gatcaggaac aattcggtat cgcgcagatg
     2101 cggcagcagt cggcgcagca ggttggcggc cttgccaccg cgcacgcggc cgagaatcag
     2161 caggcgcaat ttttcgcgct tcggtggtgc cggcaacggc agtttcgtcg catccggcca
     2221 aggcgcgatg ccgtgaggaa tcacgctttg cgcaagagcc gcaagatgcg gctgcaattt
     2281 caggaagatc gccagtgctg aactgcttgg cgcaatcagc gtcgcctgcg cggcgagcgt
     2341 cgcgtcggcc atgcgttcgc gtagcgtcag ccaccattcc ggatcgcgct cggcgaattc
     2401 gaaaccggta tccgcggcgg cgaggtcggc gcgcagttgc gcggcatcga acgtcaccga
     2461 cgcatcgtcc aggttgcggt gcagcagcgg ccacaacgga taaaaatcgt ggatgaaata
     2521 catggtcggc aaaccagtgc gcagcgcatc caggctatgg ccgatcagcg acgacacgac
     2581 caccgcatcc acattgaaat cgcgcaggat gccgtcgagg aattcgcggt agccgcgatg
     2641 cgtgagcgca gtgctggcaa tcggggcgga cagcacggct tcgcgcaacg gcaggtgccg
     2701 ccacgttgcg tcgcgaagcc gtagaacctc gccgtagcgg cggcgcgcga aattcccata
     2761 cgcggtcaac gccaaatgat ggcgcgaccg gtccgccgcg gccagatcgc gcacgaagcg
     2821 ttcgctgccg cccccccagc tatggagaat gtgcaggatt acgggtttgc ggtcgagtcc
     2881 gggatgctcg gcatgatccg acgcttgcgc cagcgctgtg gccagcgatt cgctcaactc
     2941 gccaaacggc gatgggggtg gcggattgcc gttgggcttg agttgtggcg ggccgcacag
     3001 cg
//
LOCUS       DASRVO010000153         1581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_212033, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000153 DASRVO010000000
VERSION     DASRVO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212033"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..922
                     /gene="pstB"
                     /locus_tag="VFF05_01785"
     CDS             119..922
                     /gene="pstB"
                     /locus_tag="VFF05_01785"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZW50567.1"
                     /translation="MNDLAMAAAPVAPIESARIKIAVRGLNFHYHDFHAVRNIDMNIP
                     ERKVTAIIGPSGCGKSTLLRVFNRIYAIYPKQRADGQVLLDGENILDPGYSLNRLRSQ
                     VGMVFQKPVPFPMTIYENVAYGIRHHEALSRPRMDERVEQALRAAALWDEVKDKLKRS
                     ALGLSGGQQQRLCIARGIALRPQVLLLDEPTSALDPIATGRIEQLIEELKKDYTIVIV
                     THNMQQAARVSDFTAFMYMGELIEFGDTEKIFTKPDKKQTEDYITGRFG"
     gene            1034..>1581
                     /gene="phoU"
                     /locus_tag="VFF05_01790"
     CDS             1034..>1581
                     /gene="phoU"
                     /locus_tag="VFF05_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZW50568.1"
                     /translation="MPNEHTIKSYDDELARLTGEIVRMGELAATQLEAAIDVVERRDE
                     RAAMRVVENDDAIDAFEQEVSHDVVRLLALRAPMARDLREVFSALRIASDIERIGDYA
                     ANVAKRSIPLSMVAPVAPARGLRRLSDLAVAAVRKVLDAYRDRDADKAHEVWDEDVEL
                     DDAYTGLFRELLTYMMEDPRSIT"
BASE COUNT          309 a          515 c          502 g          255 t
ORIGIN      
        1 aacgatccca atcccgcgct gcacgccatc gcttgggcgg gagcgctgct catcaccctg
       61 ttcgtgctcg cattgagcct catcgcgcgc gccgtgttcg cccgccggaa gacgcatcat
      121 gaatgacctt gccatggccg ccgctccagt cgcgccgatc gaatcggcgc gcatcaagat
      181 cgccgtgcgc ggcctgaatt tccactatca cgatttccat gcggtgcgga atatcgacat
      241 gaacatcccc gagcgcaagg tcaccgcgat catcgggccg tcgggttgcg gcaaatcgac
      301 cctgctgcgt gtgttcaacc gcatctacgc gatctatccg aagcagcgtg ccgacgggca
      361 ggtgttgctg gatggcgaga acatcctcga tccgggctac tcgctcaacc gcctgcgcag
      421 ccaggtcggc atggtgttcc agaagccggt gccgtttccg atgacgatct acgaaaacgt
      481 ggcctacggc atccgccacc acgaggcgct gtcgcgcccg cgcatggacg agcgcgtgga
      541 acaggcattg cgcgcggcgg cgctgtggga cgaggtcaag gacaagctca agcgcagcgc
      601 gctgggcctg tcgggcgggc agcagcagcg cctgtgcatc gcccgcggca tcgcgctgcg
      661 cccgcaagtg ctgcttctcg acgagccgac ctcggcactc gatcccatcg cgaccgggcg
      721 catcgagcag ctgatcgagg aattgaagaa ggattacacc atcgtcatcg tcacccacaa
      781 catgcaacag gcggcgcgcg tctccgactt caccgcgttc atgtacatgg gcgaactgat
      841 cgaattcggc gacaccgaga agatattcac caaaccggac aagaaacaga ccgaagacta
      901 catcactggt cgttttgggt gagccgtcgc gcgcgacggc gagcgcccac aacaggatgt
      961 gaagggcggg gaccacttgg cgagatccgg atgatcgagc ctggtggcag cgacagcaca
     1021 ttggagaatt ggaatgccca acgaacacac tatcaagagc tacgacgacg aactcgcgcg
     1081 ccttaccggc gagatcgtgc gcatgggcga actcgccgcg acgcaactcg aagcggccat
     1141 cgacgtggtc gagcgccgcg acgagcgcgc cgcgatgcgc gtggtcgaga acgacgacgc
     1201 gatcgacgcg ttcgagcagg aggtcagcca cgacgtggtg cgcctgctcg cgctgcgcgc
     1261 gccgatggcg cgcgacttgc gcgaggtctt ctctgcgctg cgcatcgctt cggacatcga
     1321 acgcatcggc gactacgccg cgaacgtggc caagcgttcc attccattgt cgatggtggc
     1381 gccggtggcg ccggcgcgcg gattgcgccg cttgtcggat ctcgccgtcg ccgccgtgcg
     1441 caaggtgctc gacgcctacc gcgatcgcga tgcggacaag gcccacgaag tctgggacga
     1501 ggacgtggaa ctggacgacg cctacacggg cctgttccgc gaattgctca cctacatgat
     1561 ggaagacccg cgcagcatca c
//
LOCUS       DASRVO010000154         1508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_82455, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000154 DASRVO010000000
VERSION     DASRVO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82455"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFF05_01795"
     CDS             complement(<1..559)
                     /locus_tag="VFF05_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZW50569.1"
                     /translation="MHWRGRAVFLLALLALGGCFHRHATRPSVPATQPSAAHESGGRY
                     AQANDSTPMQVPDIGKIPEPVPRDEPRSVYGNKSPYTVLGETYRVLPSCKGYDERGLA
                     SWYGNKFHGYTTSNFEKYDMYAYSAASKVLPIPCYVRVTNLENGRSAIVRVNDRGPFV
                     ANRIIDLSFVAAIKLGVYPKGTAMVE"
     gene            complement(559..>1508)
                     /gene="mltB"
                     /locus_tag="VFF05_01800"
     CDS             complement(559..>1508)
                     /gene="mltB"
                     /locus_tag="VFF05_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HZW50570.1"
                     /translation="LLNTVALAQSAADPGAAEKQFARQMAREHGLDAAAVLEILHRAT
                     YRQSVIDTMQRPAEAKPWKDYRPIFVTQRRIDDGAAFLEDNRALLERVGGQYGVPPEL
                     IAAIIGVESNYGHTPMRYRVLDALVTLAFYYPPRATYFRSELAQLLLLHSAAFPYAPE
                     ELTGSYAGAMGWPQFMPSSVAKYARDGDADGKIDLWNSLPDICASVGNYFEAFGWQTG
                     APVALRAQVAANARTLEPKGLDPVYPLQQLAEWGYTADAKLDPATPATLLRLDGVDGP
                     EFWITFRNFQVISRYNKSPLYSMAVYQLSEAIAAAARAP"
BASE COUNT          246 a          465 c          535 g          262 t
ORIGIN      
        1 cctcgaccat cgccgttccc ttcggataca cgccgagctt gatcgcggcg acgaaggaca
       61 ggtcgatgat gcggttggcg acgaacgggc cgcgatcgtt gacgcgcacg atggcgctgc
      121 ggccgttttc cagattggtc acgcgcacat agcagggaat cggcagcacc ttgctcgcgg
      181 ccgagtaggc gtacatgtcg tacttctcga agttcgacgt ggtgtagccg tggaacttgt
      241 tgccgtacca cgaggcgagt ccgcgctcgt cgtagccctt gcaactcggc agcacgcggt
      301 aggtctcgcc gagcaccgtg tagggcgact tgttgccgta gaccgagcgt ggttcgtcgc
      361 gcggcacggg ttccggaatc ttgccgatgt ccggaacctg catcggcgtg gaatcgttcg
      421 cctgggcgta gcgtccgcca gattcgtgcg ctgcggatgg ctgcgtggcc ggaaccgacg
      481 gacgcgtcgc gtggcgatgg aagcagccgc cgagcgcgag caacgcgagc aggaagacgg
      541 cgcggccgcg ccaatgcatc atggcgcgcg ggccgcggcg gcgatcgcct cgctcagttg
      601 atacaccgcc atcgagtaca gcggactctt gttgtagcgc gagatgacct ggaagttgcg
      661 gaacgtgatc cagaattcgg gaccgtcgac gccatccagg cgcagcagcg tcgccggcgt
      721 ggccggatcg agcttcgcat ccgccgtgta gccccattcg gcgagttgct gcaacggata
      781 gacgggatcc agaccctttg gctcgagcgt gcgcgcgttc gcggcgactt gcgcgcgcag
      841 cgcgacgggc gcgccggtct gccagccgaa cgcctcgaaa taattgccaa cgctggcgca
      901 aatatccggc agcgaattcc acaaatcgat cttgccgtcg gcgtcgccgt cgcgcgcgta
      961 cttggccacg ctcgagggca tgaattgcgg ccagcccatg gcgccggcgt acgagcccgt
     1021 gagctcttcc ggcgcgtacg gaaacgcggc tgaatgcaac aacagcagtt gcgcgagctc
     1081 ggaacggaaa taggtcgcac gcggcgggta gtagaacgcc agcgtgacca gcgcgtcgag
     1141 cacgcggtag cgcatcggcg tgtggccgta attgctctcc acgccgatga tggcggcgat
     1201 gagttcgggc ggcacgccgt actggccgcc gacgcgttcg agcagggcgc ggttgtcttc
     1261 gaggaaggcg gcgccgtcgt cgatgcggcg ctgggtgacg aagatcggcc ggtagtcctt
     1321 ccacggcttg gcttcggccg ggcgctgcat ggtgtcgatc acgctttgtc gataggtcgc
     1381 gcggtggagg atctccagca ctgccgcggc gtcgaggccg tgctcgcgcg ccatttgtcg
     1441 ggcgaactgt ttttccgccg cgcccggatc ggcggcggac tgggccagcg caacggtgtt
     1501 gagcagcg
//
LOCUS       DASRVO010000155         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_316276, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000155 DASRVO010000000
VERSION     DASRVO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316276"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /gene="pstA"
                     /locus_tag="VFF05_01805"
     CDS             complement(<1..751)
                     /gene="pstA"
                     /locus_tag="VFF05_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZW50571.1"
                     /translation="MSANVYRRRYAKNVVAQTLASAAALLALFFLAWILWVTVSRGVQ
                     AIGLPLFTKITAYADQGGLANAIVGSFVIDVMAIALCAPVGVLAGAYLAEYARGTRLG
                     ETIRFLNDILLSAPSIVLGLFVYVIVVLPMTALTNGSTTFSALAGSIALALIGLPVIV
                     RTTDEMLQLVPSTLREAALSLGIPQWKLTVQVLMRAAGSGILTGVLLALARLAGETAP
                     LLFTAFGNNYMAYGPLAKMASLPAAIYQYTND"
     gene            complement(748..1719)
                     /gene="pstC"
                     /locus_tag="VFF05_01810"
     CDS             complement(748..1719)
                     /gene="pstC"
                     /locus_tag="VFF05_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZW50572.1"
                     /translation="MSIATHVADPGNDRSRVDARNDALFRGVLRICALLVLAALLGAA
                     VSTLWGGREVLFGDGWHFLTSTQWNPVENRYGAWAPIYGTLVTSLIAIVIAVPLSFGI
                     ALCLTEIAPAWLRTPVATAIELLAAIPSIIYGMWGLFVFVPFMAEHVDPLLDSTLGRV
                     PLIGALFRGPPLGLGLLTAGIVLAIMIVPFISSVMREVFQTVPARLKESAYALGSTTW
                     EVVWDIVLPYTRQAVIGGIFLGLGRALGETMAVTFVLGNAYDLSASLLMPGTSISSSI
                     ANEFNEAVGLHRSALIALGFLLFVVTFVVLLIARWMLRSLKRKEGNA"
     gene            complement(1797..>2254)
                     /locus_tag="VFF05_01815"
     CDS             complement(1797..>2254)
                     /locus_tag="VFF05_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HZW50573.1"
                     /translation="VSWPTGVGGKGNEGVAAYARQIKDSIGYVEYAYALKNNIGYALM
                     TNAAGKTIKPGNAAFQAAAATADWAHAADFNLIMTNAPGADAWPITATTWVIMYKQPK
                     NAANSKVVFDFFKWSLEHGQAQAQELDYVALPEALVKQIEAYWQTQFKH"
BASE COUNT          431 a          737 c          731 g          355 t
ORIGIN      
        1 gatcgttggt gtattgatag atcgccgccg gcaggctggc cattttcgcg agcgggccgt
       61 aggccatgta attgttgccg aacgcggtga acagcagcgg cgcggtttcg ccggcgagcc
      121 gcgccagcgc aagcagcacg ccggtgagaa tgcccgagcc cgccgcgcgc atcagcacct
      181 gcacggtcag cttccattgc ggaatgccga gcgacagcgc ggcctcgcgc agcgtcgacg
      241 gcacgagctg cagcatttcg tcggtggtac gcacgatcac cggcagcccg atcagggcga
      301 gcgcgatgga gccggccagc gcggagaacg tcgtcgagcc attcgtcagc gcggtcatcg
      361 gcagcaccac gatcacgtac acgaacagac cgagcacgat ggacggcgcg gagagcagga
      421 tgtcgttgag aaaacggatt gtctcgccca gtcgcgtgcc gcgcgcgtat tcggccaggt
      481 acgcgccggc cagcacgcca accggcgcgc acagcgcgat cgccatcacg tcgatgacga
      541 agctgccgac aatggcgttg gccaggccgc cctggtcggc ataggcggtg attttcgtga
      601 acagcggcaa cccgatcgcc tgcacgccgc gactcaccgt cacccacagg atccaggcca
      661 ggaagaacag ggccagcagc gcggccgcac tggccagcgt ctgcgccacg acgtttttcg
      721 cgtaacggcg acggtagacg ttcgcgctca tgcgttgcct tccttgcgct tgagcgagcg
      781 caacatccag cgcgcgatca acagcacgac gaacgtcacc acgaacagca ggaaccccag
      841 tgcaatcagg gcggagcgat gcaggcccac cgcctcgttg aattcgttgg cgatggacga
      901 cgagatcgac gtgcccggca tcagcagcga ggcgctgagg tcgtaggcgt tgccgaggac
      961 gaacgtgacc gccatggttt cgccgagcgc gcggcccaga ccgaggaaga tgccgccgat
     1021 gacggcctgc cgcgtatacg gcaggacgat gtcccagacc acttcccatg tcgtggaacc
     1081 caaggcgtac gccgattcct tcaggcgcgc gggcacggtc tggaacacct cgcgcatcac
     1141 cgaggatatg aacggcacga tcatgatcgc cagcacgatg ccggccgtga gcagacccag
     1201 ccccagcggc gggccccgga acagcgcgcc gatcagtggc acgcggccga gcgtggaatc
     1261 cagcaacgga tcgacgtgct ccgccatgaa cggcacgaac acgaacagtc cccacatgcc
     1321 gtagatgatc gacggaatcg cggcaagcaa ttcgatggcg gtggcgaccg gtgtgcgcag
     1381 ccacgccggc gcgatttcgg tcaggcacaa ggcaattccg aagctcagcg gcacggcaat
     1441 gacgatggcg atcagcgagg tgaccagggt accgtagatc ggcgcccagg cgccgtagcg
     1501 gttctcgacg ggattccatt gcgtgctggt caggaaatgc cagccatcgc cgaacagtac
     1561 ctcgcgaccg ccccacagtg tggacacagc cgcaccaagc agcgcggcca ggacgagcaa
     1621 ggcacagatc cgcagaacgc cgcggaacag cgcgtcgttg cgcgcgtcga cgcggctgcg
     1681 gtcgttgccg ggatcggcga cgtgggtggc gatcgacatg ctcagtcccg tgaaaaagcc
     1741 aggcacgccg cccgagtgcg gcgtgccgtc cttcatttta cttcacgcca gtccgctcag
     1801 tgcttgaact gcgtctgcca atacgcttcg atctgcttga ccagcgcttc gggcagggcc
     1861 acgtaatcga gttcctgcgc ctgcgcctgg ccgtgttcca gcgaccactt gaagaagtcg
     1921 aacaccacct tggaattcgc cgcattcttc ggctgcttgt acatgatcac ccaggtcgtc
     1981 gccgtgatcg gccatgcgtc ggcgccgggc gcgttggtca tgatcaagtt gaagtctgcc
     2041 gcatgtgccc aatccgcggt tgccgcggcg gcctggaaag cggcattgcc cggcttgatc
     2101 gtcttgccgg cggcgttcgt catcagcgca tagccgatgt tgtttttcag cgcataggcg
     2161 tattcgacgt agccgatcga gtccttgatc tgcctggcgt aggccgcgac gccctcgttg
     2221 cccttgccgc cgacgccggt cggccagctg accg
//
LOCUS       DASRVO010000156         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_82490, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000156 DASRVO010000000
VERSION     DASRVO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82490"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VFF05_01820"
     CDS             complement(<1..880)
                     /locus_tag="VFF05_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50574.1"
                     /translation="MSMHNRRCSDFTVSRPHVLAYLLALALPVLPAPACAITFTVDTT
                     NDTVSGGALSGSLRDGIIAVNASTDATNTIQFSSGLGSNAHIVLASPLPLVLNNVNID
                     GTGVYPYIDGDNLYRIFFIGVDVATQGSLQAQFPTAPLGNRLSVTLRNLYLENGRAKG
                     GNGGGGGMGAGGAVFVNSASDVVMDSVDLVNNQAVGGNGSATVGTNPQQLGGGGGLGG
                     NGGKPSSDSNGGGGGIFGSGGVGSDSAGGGIFGNGGGFVGGGGGFSGDGGGANGNGAD
                     GSFVLAGMTGTGGGGSG"
     gene            complement(966..>2480)
                     /locus_tag="VFF05_01825"
     CDS             complement(966..>2480)
                     /locus_tag="VFF05_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW50575.1"
                     /translation="PGSNGIEIAQRIRESGHAFLPIVFLSGELDPDLRFDAIRMGADD
                     FITKPVKPRHLVVAVESRIRRARELSAAHTDSRSERRGNLSGRDVLVAEVLRAAREES
                     ERCPALALVAVDDGEGVLRSVGFAAGSTLAQQLAGTLAAEIGGQRTLCAWGELKFLVL
                     LHADDQLTLREQLENLRRKLDERAWLSEQTPVHLRLTLACVRLHPQLADVGEVLDRVR
                     AMLAKAQAAGGARCEFDLREATTHADEDPQVRLVRALLRAPSARGTAQFEFQPFVPLA
                     GQIAGQYEARMALKPPKSRQGLLLQRCDWLPIARKLDMIAHADRHFLRGVIERVREKH
                     VNGEDLRLYLPVAASSLFDPAFAPWLAAEFGAHAVPSHVVALEFDAAEARTELPRLRG
                     ALEPVQRVGVRLALNVDAADAADLGKLLAIDAFGVVKFARAGDADTKPETAWEPCSKA
                     IAEARSLGKIVVASGVAGMADIGVLLRFGVHYAQGDALSGWLAEWDFDFAEAEL"
BASE COUNT          472 a          852 c          749 g          407 t
ORIGIN      
        1 caccgcttcc gccgccaccc gtgccggtca tcccggcgag aacaaagctg ccgtcggcgc
       61 catttccgtt tgcaccgccg ccatcacccg aaaacccacc accaccgccg acgaaaccac
      121 caccattgcc gaagatgcca ccacccgcgg aatcgctgcc aacgccaccg ctgccaaaaa
      181 tcccgccgcc accgccgttg ctgtctgaag acggtttacc gccgttgcca cccaatccgc
      241 caccgcctcc cagctgttgt ggattggttc cgactgtcgc cgatccattg ccgccaacgg
      301 cctgattgtt gaccagatcc acgctgtcca tgacgacatc ggaagcgctg ttcacgaata
      361 cggccccacc tgcgcccatg cctccgccgc cgccattgcc acccttggca cgaccgtttt
      421 ccaggtacag gttgcgcaag gtcacgctca agcgattgcc gagcggcgcg gtcggaaact
      481 gcgcttgcag cgagccttgc gtggcgacat ccacgccgat gaagaagatg cgatacagat
      541 tgtcgccatc aatgtaggga tacacgcccg tgccatcgat gttgacgttg ttgagaacaa
      601 ggggcagcgg gctcgccagc acgatgtggg cattgctgcc cagtccgctg ctgaactgga
      661 tggtgttggt cgcatccgtc gaagcgttga ctgcaattat gccgtcgcgc agcgaaccgc
      721 ttagcgcccc accgctgacg gtatcgttcg tggtgtctac ggtaaatgtg atcgcgcagg
      781 ctggcgctgg gagcaccggc aatgccaatg caagcagata ggccagtacg tgaggacgcg
      841 agaccgtgaa atccgagcag cgacgattgt gcatggacat gacaccctcc gtcagtggga
      901 aagccggctt gtcctgccta aacgagaaat gggacgctgg cctatcactc gtgcggcaag
      961 cgaatctaca actccgcttc ggcgaaatcg aaatcccact cggccagcca gccgctgagc
     1021 gcatcgccct gcgcataatg cacgccgaac cgcagcaaga cgccgatatc ggccatgccg
     1081 gcaaccccgc tcgcaaccac gatcttgccc agcgagcgcg cttcggcgat ggccttggag
     1141 cacggctccc aggcggtttc gggcttggtg tccgcgtcgc cggcacgtgc gaacttgacc
     1201 acgccgaacg catcgatcgc aaggagtttg ccgagatccg ccgcgtcggc agcgtccacg
     1261 ttcaatgcca ggcgcacacc gacgcgctgc acgggttcaa gcgcaccgcg caggcgcggc
     1321 aactcagtgc gcgcttcggc ggcgtcgaat tccagcgcaa cgacatggct cggcaccgcg
     1381 tgcgcgccga actctgctgc gagccagggc gcgaacgccg ggtcgaaaag actcgacgct
     1441 gcaaccggca agtacaaacg caaatcttcg ccgttcacgt gcttctcgcg cacgcgctcg
     1501 atcaccccgc gcaggaaatg ccggtccgca tgcgcgatca tgtccagctt tcgcgcgatc
     1561 ggcagccaat cgcagcgctg caacagcagg ccttgccgcg atttcggcgg cttgagcgcc
     1621 atgcgcgcct cgtactggcc ggcaatctgc ccggccagcg gcacgaacgg ctggaattcg
     1681 aattgcgcgg tgccgcgcgc actcggcgcc cgcaacaacg cacgcacgag acgtacttgc
     1741 ggatcttcgt cggcgtgggt agtggcctcg cgcaggtcga attcgcagcg cgcaccgccc
     1801 gccgcttgcg ctttcgcgag catggcgcgc acgcggtcca gtacttctcc gacgtcggcg
     1861 agttgcggat gcaaacgcac acaggccaga gtcaggcgca gatgcacggg tgtctgctcg
     1921 gataaccacg cgcgctcatc gagcttgcgc cgcaagttct cgagctgctc gcgcaatgtc
     1981 agttgatcgt cggcgtgcag aaggacgaga aacttcaatt cgccccaggc gcacaaggtg
     2041 cgttggccgc cgatctcggc tgccagcgtg cccgccaact gctgcgcgag cgtgctgccg
     2101 gcggcgaacc cgacactgcg cagcacgcct tcgccgtcgt ccacggcaac cagggccaat
     2161 gccgggcaac gctcggattc ctcgcgcgcg gcgcgcaaca cttcggccac gagcacatcg
     2221 cgaccggaaa gattgccgcg gcgctcgctg cgactgtcgg tgtgtgcggc ggacagctca
     2281 cgggcacgcc ggatgcggct ttcaacagcc acgaccagat gccggggttt gaccggtttg
     2341 gtgatgaaat cgtccgcgcc catgcgaatc gcgtcgaatc gcaaatccgg atcgagctcg
     2401 ccggacagga acacgatcgg caaaaaggcg tgcccggatt cgcggatgcg ctgcgcgatc
     2461 tcgatgccgt tgctgccggg
//
LOCUS       DASRVO010000157         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_290369, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000157 DASRVO010000000
VERSION     DASRVO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290369"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFF05_01830"
     CDS             <1..563
                     /locus_tag="VFF05_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50576.1"
                     /translation="CAHRGAWFLVFVLFAFLLAGSGLALRGYSLLSAEAPVANVQSRQ
                     LGPQWFGVRVDLPDGTHRSADLHGDEWQLDARVIKWAPRAVALGAQPLFRVDRLSGRY
                     RDTAQAQATLPSAIALDADSVVDLWQMKQRFPRWLPWIDADYGSAAFLPLVDGGSYSV
                     TISPLGGLVARPADAATADKLKALGW"
     gene            complement(567..1184)
                     /locus_tag="VFF05_01835"
     CDS             complement(567..1184)
                     /locus_tag="VFF05_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50577.1"
                     /translation="MKPPFAIFIAIALAAAPVCAATKDAPSRLAGKQCAWEMVSDAAL
                     GLEASVQRCDYGFRKVHLYAKGNALLMHYSDGGEDEKLIETFVLKDKETPREGIKRVF
                     DEHTPDKFLAARCLVQPYQSEGPVPKGAKRYTVLPNAALKKELDAKQDPGDIPEPPCG
                     EWGDMPDSVQYYEAQEGVGRIMIVRAGQDEPMFDEKTLRLLPPKK"
     gene            complement(1181..1681)
                     /locus_tag="VFF05_01840"
     CDS             complement(1181..1681)
                     /locus_tag="VFF05_01840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZW50578.1"
                     /translation="MSDAYRIRHAMPADLPALVALERRAFSTDHLSPRQYRHHLGNPA
                     ALVLAAVDGTGLLGKAVVFFRKGSDIARLYSIAVDDAARGRGIASALLHAVERGARAR
                     GCARLRLEVRQDNPGAIRLYEKLGYRRFGAIAGFYEDGADAWRYEKILRARDVRTAAR
                     ERTTTS"
     gene            1897..3381
                     /locus_tag="VFF05_01845"
     CDS             1897..3381
                     /locus_tag="VFF05_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family protein"
                     /protein_id="HZW50579.1"
                     /translation="MSRLVIVVEKATDWGSYFPSENVVAAQDYLKEAISVDDERVQVI
                     NLCRSYKYLGVGYYVSLLAEARGHKVIPSVRTINDLRKRALYGLDIEDLNQKLTNFLP
                     AGGRDTTDLGVLVYFGETSYAALADLARQVFETFPCPILRLEFEREKVWQISAIKPAS
                     LHTLAEPQEDSFAAALDKFSKKLWRKPRARRQYRYDIAMLADPNEQMPPSNKRALKLF
                     AEAGRELAIEVETIGKNDYSSLAEYDGLFIRETTALDSHTYRFANKAEKEGMVVIDDP
                     GSILKCTNKIYLHDLLKSRKLPTPKTEILYRDDGKRLEELPDLLGFPIVLKIPDGSFS
                     RGIVKVETTAQLKVAVDDLFQHTALVLAQEFLFTEFDWRIGVLDGEALYACQYFMSRG
                     HWQIYNHGANATQKSGGFKTLPVRSAPADIVKLALKATAPIGDGLYGVDLKQIGNRNV
                     VIEVNDNPSIDAGVEDAYLGEDLYRRIMSEFLRRMEKKRLGIKD"
     gene            complement(3297..3692)
                     /locus_tag="VFF05_01850"
     CDS             complement(3297..3692)
                     /locus_tag="VFF05_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50580.1"
                     /translation="MRPSQMKTGWAMSTLSNPAVRAGAFGEAVLWMWLAAGILACLIF
                     PALRTQDALLGWLLFWLIAAPLIDLGLIHRNGLSTAARTFLVSRARRRRPARQARRLR
                     RDQSLMPRRFFSMRRRNSDMMRRYKSSPR"
BASE COUNT          686 a         1217 c         1178 g          678 t
ORIGIN      
        1 cctgcgcgca tcgcggggcg tggttcctgg tgttcgtgtt gttcgctttc ctcctcgctg
       61 gatcgggcct ggctttgcgc ggctacagcc tgctcagcgc cgaagcgccg gtcgcgaacg
      121 tgcagtcgcg ccagcttggt ccgcagtggt tcggcgtgcg cgtggatttg cccgacggca
      181 cacatcgcag cgcggatctg cacggcgacg agtggcagct cgatgcgcgc gtgatcaagt
      241 gggcgccgcg tgcggtcgcg ctcggcgcgc agccgctgtt ccgcgtggat cgcctgtccg
      301 gccgctatcg cgacaccgca caggcgcagg cgacactgcc aagtgcgatt gcgctggatg
      361 cggattccgt cgtcgacctg tggcagatga agcaacgctt cccgcgctgg ctgccttgga
      421 tcgacgccga ctacggcagc gcggccttcc tgccgctggt cgatggcggc agttacagcg
      481 taaccatcag tccgctcggt ggactcgtcg cacggccggc ggacgcggcg acggcggaca
      541 agctcaaggc gctgggctgg tgagcgctat tttttcggcg gcaacaggcg cagcgtcttc
      601 tcatcgaaca tcggctcgtc ctgtcccgcg cgcacgatca tgatgcggcc cactccctcc
      661 tgcgcctcgt agtactggac gctgtccggc atgtcgcccc attcgccgca tggcggctcg
      721 ggtatgtcgc ccgggtcctg ttttgcgtcg agttccttct tcagcgcggc gttcggcaga
      781 accgtgtagc gtttcgcacc cttgggtacc ggaccctcgc tctgataggg ttggacgagg
      841 caacgcgcag ccaggaattt gtccggcgtg tgttcgtcga atacgcgctt gatgccttcg
      901 cgcggcgttt ccttgtcctt gagaacgaac gtctcgatca gcttttcgtc ctcgccgccg
      961 tcggaataat gcataagcaa ggcgttgcct ttcgcgtaga gatggacctt gcggaagccg
     1021 taatcgcagc gctgcacgct cgcttcgagc ccgagcgcgg cgtcggaaac catttcccac
     1081 gcgcattgct tcccggccag cctcgatggt gcgtctttcg tcgcggcgca tacgggagct
     1141 gcggccaacg cgatggcgat gaaaatggcg aatggtggtt tcatgatgtc gttgtacgtt
     1201 cccttgcggc ggtccggaca tcgcgtgccc ggaggatttt ctcgtaccgc cacgcatccg
     1261 cgccgtcttc gtagaatccg gcgatggcgc cgaaacggcg gtaaccgagt ttttcgtaga
     1321 gtcgaatcgc accggggttg tcctggcgta cttcaaggcg cagccgcgcg catcctcttg
     1381 cgcgtgcgcc gcgctcgaca gcatgcagca acgcgcttgc gatgccgcgc ccgcgcgctg
     1441 catcgtccac ggcgatggaa tacaaccgtg cgatgtcgct gcccttgcgg aaaaacacca
     1501 cggccttgcc gagcaaaccc gtgccatcga ccgcggcgag cacgagcgcc gccggattgc
     1561 ccagatggtg ccggtactgg cgcggcgaca gatgatcggt gctgaaggcg cgccgttcca
     1621 gcgcgaccag cgcgggcagg tcggcgggca tggcgtggcg gatgcgataa gcgtcggaca
     1681 tggcgccgca gcgtaggcta gcgccatgcg tgacgcaatg ctgaacgtgc gacgccgcac
     1741 gcttgcgcgg ctttgcggct gtgtcaaact agccgccccg tgccttgcgc cgatgccaat
     1801 gtccgaaccc ccgagtctat atttccagcg ggcttccctg cccgctccag agcggcaatc
     1861 acggccgtgc ttttcaattt tgtgcaatag ccacgcatga gcagattagt catcgtggtg
     1921 gaaaaggcga cggattgggg ttcgtacttc ccctcggaaa atgtcgtcgc cgcgcaggat
     1981 tacctgaaag aagcgatcag cgtcgacgac gaacgcgtgc aggtgatcaa cctgtgccgc
     2041 agctacaaat acctcggcgt cggctattac gtttccctgc tggccgaagc gcgcgggcac
     2101 aaagtgattc cgtcggtgcg cacgatcaac gacctgcgca agcgcgcact gtacggcctg
     2161 gacatcgaag acctcaacca gaagctgacc aactttttgc cggcaggcgg gcgcgacacc
     2221 accgatctgg gcgtactcgt gtacttcggc gaaaccagct acgcggcgtt ggcggacctg
     2281 gcgcggcagg tgttcgagac gtttccgtgc ccgatcctgc gcctggaatt cgagcgcgag
     2341 aaggtctggc agatcagcgc gatcaagccg gccagcctgc acacgctggc cgaaccgcag
     2401 gaggattcct tcgctgctgc gctcgacaag ttttcgaaaa aactctggcg caagccgcgc
     2461 gcgcgccgcc agtaccgcta cgacatcgcc atgctggccg atccgaacga gcagatgccg
     2521 ccgtcgaaca agcgcgcgct gaaactcttc gccgaggcgg gtcgcgaact cgccatcgaa
     2581 gtggaaacga tcggcaagaa cgactactcg agcctggccg aatacgacgg cctgttcatc
     2641 cgcgaaacca ccgcgctgga tagccatacc tatcgcttcg ccaacaaggc cgagaaggaa
     2701 ggcatggtgg tgatcgacga tcccggctcc atcctcaagt gcacgaacaa gatctacctt
     2761 cacgacctgc tcaagtcgcg caagctgccc acgccgaaaa ccgagatcct ttaccgcgac
     2821 gacggcaagc gcctggagga attgcccgac ctgctcggct ttccgatcgt gctgaaaatt
     2881 cccgacggat cgttttcgcg cggcatcgtc aaggtggaaa cgaccgcgca gctcaaagtc
     2941 gccgtggacg acctgtttca gcacaccgcg ctggtgctgg cgcaggaatt cctgttcacc
     3001 gagttcgact ggcgcattgg cgtgctcgac ggcgaggcgc tgtacgcctg ccagtacttc
     3061 atgtcgcgcg gccactggca gatctacaac cacggcgcga acgcgacgca gaaatccggc
     3121 ggcttcaaga ccctgccagt gcgcagcgcg ccggccgata tcgtcaaact cgcgctgaag
     3181 gccaccgcgc cgatcggcga cggactctac ggcgtggacc tcaagcagat cggcaaccgc
     3241 aacgtggtga tcgaagtcaa cgacaatcct tccatcgacg ccggcgtgga agacgcctat
     3301 ctgggcgagg atttgtaccg gcgcatcatg tccgaattcc tgcgccgcat ggagaaaaaa
     3361 cgcctcggca tcaaggactg atcgcggcgc aggcgccgcg cctgccgggc gggacgacgg
     3421 cggcgcgcac gactgacgag gaacgtgcgc gctgctgtcg acaacccatt gcggtggatc
     3481 agccccaaat cgattagcgg cgcggcgatc agccagaaca gcagccagcc gagcaacgca
     3541 tcttgcgtgc gcagcgccgg aaagatcagg cacgccagga tgccggccgc cagccacatc
     3601 caaagaacag cctctccgaa cgcacctgcg cgaacggccg gattcgataa cgtcgacatg
     3661 gcccaccccg tcttcatctg cgatggacgc atcctcgcaa ggcgcgatct cacgcattga
     3721 gatggcatgt cgaggcggtg cgctcgtcta tactgcgca
//
LOCUS       DASRVO010000158         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_134266, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000158 DASRVO010000000
VERSION     DASRVO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134266"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..>1557
                     /gene="ppsA"
                     /locus_tag="VFF05_01855"
     CDS             76..>1557
                     /gene="ppsA"
                     /locus_tag="VFF05_01855"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HZW50581.1"
                     /translation="MSDLILWLDELRLSDLGKVGGKNASLGEMIGNLAKLGVSVPGGF
                     ATTADAFKQFIAQSGLAERIQAKLAALDVDDVGALTKAGAEIRQWVIDTPLIPAFDKA
                     VRTAYKILCDKAGVNDTAVAVRSSATAEDLPDASFAGQQETYLNVSGADDVIHKVKEV
                     FASLYNDRAIAYRVHHGFAHEDVFLSAGVQLMVRSDIGASGVLFTLDTESGFRDAVFV
                     TASYGLGEMVVQGAVNPDEFYVYKPTLKLGKPAILRRQVGAKQQRMVYSNKPGERVRI
                     EETPASDRAKFCVTDADVHELAKQALVIEQHYGRPMDVEWAKDGNSGKLYIVQARPET
                     VKSRARAAQIERFQLKGKGDVLAEGRAIGQKIGAGTARVIRSLADMNRVQAGDVLVAD
                     MTDPDWEPVMKRAAAIVTNRGGRTCHAAIIARELGVPAVVGTGNALEQIPDGAQVTVS
                     CAEGDTGFIYSGKLEFERVTADLDKMPPAPLKIMMNVANPERAF"
BASE COUNT          294 a          520 c          501 g          242 t
ORIGIN      
        1 tgtcttgtcg gtcgcaaagc ccgaaaatcg gcactttccg ggctccacgc gccggacaat
       61 tcctggagat tccccttgag cgacctcatc ctttggctcg acgagctgcg cctgtccgat
      121 ctgggcaagg tcggcggcaa gaatgcctcg ctcggcgaga tgatcggcaa cctcgccaag
      181 ctcggcgttt cggtgccggg tggtttcgcg accaccgcgg acgccttcaa gcaattcatc
      241 gcgcaaagcg gactcgccga acgcatccag gcaaaactgg ccgcactgga tgtcgacgat
      301 gtcggcgcac tgaccaaagc cggcgcggaa atccgccaat gggtgatcga caccccgctc
      361 atccccgcct tcgacaaggc ggtgcgcacg gcctacaaga tcctgtgcga caaggcggga
      421 gtgaacgaca ctgccgtggc cgtgcgttcg tccgcgaccg ccgaggacct gcccgatgcc
      481 agttttgccg gccagcagga aacctacctg aacgtcagcg gcgcagacga cgtcattcac
      541 aaggtgaagg aggtcttcgc ctcgctgtac aacgatcgcg ccatcgccta tcgcgtgcac
      601 cacggattcg cccacgagga cgtgttcctc tccgccggcg tgcagctcat ggtgcgctcg
      661 gacatcggcg cttcgggcgt gctgttcact ctggacaccg aatccggatt ccgcgacgcc
      721 gtgttcgtga ccgcgagtta tggcctcggc gagatggtcg tgcagggcgc ggtgaatccg
      781 gacgaattct acgtctacaa gccgacgctg aagctcggca agccggcgat cctgcgccgc
      841 caggtcggcg ccaagcaaca gcgcatggtg tattcgaaca aacccggcga acgcgtgcgc
      901 atcgaagaaa cgcccgcttc cgatcgcgcg aaattctgcg tcaccgatgc cgacgtgcac
      961 gagctcgcca agcaggcgct ggtgatcgaa cagcactacg gccggccgat ggacgtggaa
     1021 tgggccaagg acgggaacag tggaaaactc tacatcgtgc aggcgcggcc ggaaacggtg
     1081 aagtcgcgcg cgcgcgccgc gcaaatcgag cgtttccagc tcaaaggcaa gggcgacgtg
     1141 ctggccgagg gccgcgcgat cggccagaag atcggcgccg gcaccgcgcg cgtgatccgc
     1201 tcgctcgcgg acatgaaccg cgtgcaggcg ggcgacgtgc tcgtggccga catgaccgac
     1261 cccgactggg agccggtgat gaagcgcgcc gcggccatcg tcacgaaccg cggcggccgt
     1321 acctgccacg ccgccatcat cgcgcgcgag ctgggcgtgc cggccgtggt cggcaccggc
     1381 aacgcgctcg agcagatccc cgatggcgcg caggtcacgg tgtcctgcgc cgagggcgat
     1441 accggcttca tctattccgg caagctcgaa ttcgagcgcg tcaccgcgga cctcgacaag
     1501 atgccgccgg cgccgctcaa gatcatgatg aacgttgcca atcccgagcg cgcgttc
//
LOCUS       DASRVO010000159         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_290538, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000159 DASRVO010000000
VERSION     DASRVO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290538"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFF05_01860"
     CDS             complement(<1..1065)
                     /locus_tag="VFF05_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZW50582.1"
                     /translation="MAKMKARDDHDALLAGRHVLVTGGTGSLGAAVVERMLSGRHGQP
                     ERIVVFSRDEAKQHELRQRWLRNLAATDAEIWRESTTRRVQFRVGDVRDRDALASALD
                     GVDTLIHAAALKQVPTCEYFPAEAVRTNVLGTENIVAAARGARAHLRCVVGISTDKAV
                     KPVNAMGMTKALAERILLRATLDIPECRFSIVRYGNVAASRGSVLPLFLEQTARGGPI
                     TVTDPSMTRFLLSLDEAVDAVMRALAHGLPGETLVPRLPAARVADIARAVAGNRPIEI
                     RVTGIRPGEKIHEVLIAAEERPRTTMRGRDFCVASILPEIADAQCDTAADLPFAAEYS
                     SADATIGVEGVRKLLEAAGLL"
     gene            1208..>2122
                     /locus_tag="VFF05_01865"
     CDS             1208..>2122
                     /locus_tag="VFF05_01865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW50583.1"
                     /translation="MAATQTDGAVDLVTIVIPVFNGADYLAQAIDSALAQTWHAVEVL
                     VVDDGSEDAGRTRAIAESYGDRIHLLCRPHGGVAAALNAGIAAMRGAWFSWLSHDDVY
                     DPRKIEMQLNALRAASPGTLAFSDYATMDAEGSLLRIEHATCGFDASRRLWSVLEGKI
                     NGCTLLIPRHLLVEAGGFDIRLPTTQDYELWFRLVRRNPLIAVPGVVVRQRMHDGQGS
                     RTRRHLDEAGLLWREMLESLAPAEMRQLAGSEAAFRVRMARHLATSPYPAAAATAAEL
                     LRAELGRIKATLVWTAAQTPGPERMLAAL"
BASE COUNT          368 a          697 c          687 g          370 t
ORIGIN      
        1 gagcaatccc gccgcttcga ggagcttgcg taccccctcg acgccaatcg ttgcgtccgc
       61 cgaactgtat tcggccgcga agggcaggtc cgcagccgtg tcgcactggg cgtctgcgat
      121 ctcgggcagg atggaagcga cgcagaaatc gcggccgcgc atggtggtcc gcggccgctc
      181 ctcggccgcg atgagcacct catggatctt ctcgccgggg cgaatgccgg tgacgcggat
      241 ctcgatcgga cggttaccgg caaccgcccg ggcgatatcc gcgacgcgcg ccgccggcag
      301 acgcggcacc agggtctcgc cgggcaggcc gtgcgcaagc gcacgcatga cggcatccac
      361 ggcttcgtcc aaactcagca ggaatcgggt catgctcgga tctgtcaccg ttatcggccc
      421 acccctcgcc gtctgctcca gaaaaagcgg cagcacggaa ccgcgtgagg cagcgacgtt
      481 gccgtagcgg acgatggaaa aacggcactc cggaatatcg agcgtggcac gcagcaaaat
      541 gcgctcggcc agtgccttgg tcatgcccat ggcattgacc ggcttcaccg ccttgtccgt
      601 cgaaatgccg accacgcagc gcaggtgcgc gcgggcgccg cgtgccgcag cgacgatgtt
      661 ttccgtaccg agtacgttgg tgcgcaccgc ttcggccgga aaatattcgc aggtcggcac
      721 ctgcttgagc gccgcggcgt gaatcaaggt gtcgacgcca tccagagccg acgccagtgc
      781 atcgcgatcg cgaacgtcgc cgacgcgaaa ctggacgcgc cgggtcgtgg attcgcgcca
      841 gatctcggca tccgtcgccg caagattgcg caaccagcgc tgccgcaatt cgtgttgctt
      901 ggcctcgtcg cgcgaaaaaa ccacgatgcg ctcgggttgg ccatggcgtc cggacaacat
      961 gcgttcgacc accgccgcgc cgagggagcc ggtgcctccg gtgaccagga catgccggcc
     1021 cgcaagcagc gcatcgtggt catcgcgagc tttcatcttc gccatgctcc tttcccagtt
     1081 ccgtcggaca tcggtaatcg cgaccgcgcc atttatgcta atttctgcag ggtaacccaa
     1141 cgtagcactt gttttccaac cgcgctgccc ggagctgcag cgtattcgtt caggcatggc
     1201 tggcgacatg gcggcaaccc aaacagacgg cgcggtcgat ctcgtcacca ttgtcattcc
     1261 cgtcttcaac ggcgcggact atctggctca ggccattgac agcgcgctcg cccagacctg
     1321 gcatgcggtc gaggtcctgg tcgtcgacga cggttcggag gacgccggac gcacgcgcgc
     1381 catcgccgaa tcgtacggcg accgcatcca cctgctgtgc cggccgcatg gaggtgtcgc
     1441 tgcggcgctg aatgccggaa tcgcggcgat gcgcggcgcc tggttctcgt ggctcagcca
     1501 cgacgatgtg tacgatccgc gcaagatcga aatgcagctg aacgctctgc gcgcagcgtc
     1561 gccgggcacg ctggcgttca gcgactatgc gacgatggat gcggagggga gtctcttgcg
     1621 tatcgagcac gcgacctgcg gcttcgatgc atcgcgccgt ttgtggtccg ttctggaagg
     1681 aaagatcaac ggttgtaccc ttctcattcc gcgccacctg ctcgtcgagg cgggcgggtt
     1741 cgatatcaga ttgccgacca ctcaggacta tgagctgtgg ttccgcctgg tgcgcaggaa
     1801 tccgctgatt gcggttcccg gcgtcgtcgt ccgacaacgg atgcacgacg ggcaaggatc
     1861 gcgcacacgc cgccatctcg atgaggctgg attgctttgg cgggaaatgc tcgagtccct
     1921 cgcgcctgcg gaaatgcggc agctcgccgg atcggaggcg gcgttccggg tccgcatggc
     1981 acggcacctg gcaacttcgc cgtatccagc cgctgcggcg acagccgcag aattgctgcg
     2041 ggcggaactg ggacgcatca aggcaacctt ggtgtggacc gccgcgcaaa cacccggtcc
     2101 ggagcgcatg ctggcggcac tc
//
LOCUS       DASRVO010000160         1447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_290541, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000160 DASRVO010000000
VERSION     DASRVO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290541"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VFF05_01870"
     CDS             <1..1141
                     /locus_tag="VFF05_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZW50584.1"
                     /translation="SVDADFSSSRQKQPGSLAPVPNNNAFLYSLFTPQLSVSYVPDVF
                     GLNRRTVESLQAQAQAARFQLIAAHVALAANVVNAAVGEAALREQVKATEALVDIETQ
                     SVEILRYQFDKGYASRLDLAAQESQLAQTQAGLPPLRKQLEQQRHLIAALAGEFPAQA
                     RDADFDLASLKLPQDLPLSLPSQLVEQRPDVRQAQANWHAASAQVGVAIASRLPQITL
                     SANAGSTALQIADVFKAGTGFWSVGLDLAAPIFQGGKLLHAEHAAKAQLVQAAAQYRS
                     TVLTAFQNVADTLTALDEDAKALQAAAAAADAAKVTLDLAQRQTQSGYANELSLLNAQ
                     QAWQQARIALIQAQANRYADTAALYLALGGGWWHDADLSGKNDED"
     gene            1131..>1447
                     /locus_tag="VFF05_01875"
     CDS             1131..>1447
                     /locus_tag="VFF05_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW50585.1"
                     /translation="MKIEAFDSSAPRWMAWLVAAGMFLGGCSSGGSVNAPASAAPGDV
                     KLTAAQMQHVQLYTVAEAQFHKATRANGTVDFDQERSTSVLAPISGPVTKLLVAPGDR
                     VKKG"
BASE COUNT          256 a          477 c          488 g          226 t
ORIGIN      
        1 atcggtcgac gcggattttt cgtcgagccg gcagaagcag cccggctcgt tggcgccggt
       61 gccgaacaac aacgctttcc tgtacagcct gttcacgccg cagctgagcg tgtcctacgt
      121 gccggacgtg ttcggcctga accggcgcac ggtggaatcg ctgcaagcgc aggcacaggc
      181 cgcgcgcttc cagctgatcg cggcgcacgt cgcgctggcc gcgaacgtcg tcaatgcggc
      241 ggtgggcgag gccgcgctgc gcgagcaggt gaaggcaacc gaagcgctgg tcgacatcga
      301 aacgcaatcc gtcgaaatcc tgcgctacca gttcgacaag ggttacgcga gccggctcga
      361 tctcgccgcg caggaatcgc agcttgcgca gacccaggcc ggcctgccgc cgctgcgcaa
      421 gcaacttgag caacagcgcc atctgatcgc ggcgcttgcc ggcgagtttc cggcgcaggc
      481 gcgcgatgcc gatttcgacc tggcgagttt gaagctgcct caagacttgc cgttgagcct
      541 gccgtcgcaa ctggtcgagc aacgccccga tgtgcgccag gcgcaggcga actggcatgc
      601 ggccagcgcg caggttggcg ttgccatcgc cagccgactg ccgcagatca ccctgagcgc
      661 gaacgctggc agcacggcgt tgcagatcgc cgacgtgttc aaggccggca ccggtttctg
      721 gagcgtgggc cttgatctcg ccgcgccgat cttccagggc ggcaagctgc tgcacgccga
      781 gcatgcggcg aaggcgcagc tggtgcaggc cgcggcgcaa taccgcagca cggtgttgac
      841 cgcgttccag aatgtcgccg acacgctgac cgcgctcgac gaagatgcga aggcattgca
      901 ggccgctgct gccgccgcgg atgcagccaa ggtcacgctc gacctggcgc agcggcagac
      961 gcagtcgggc tacgcgaacg aactttccct cctcaacgcg cagcaggcct ggcagcaggc
     1021 gcgcatcgcc ctgatccagg cgcaggccaa tcgctacgcc gacaccgcgg ccttgtatct
     1081 cgcgctcggc ggcggctggt ggcacgacgc ggacttatcc gggaaaaacg atgaagattg
     1141 aagcattcga ttcgagcgcg ccgcgttgga tggcgtggct ggttgccgct ggaatgttcc
     1201 tcggcggatg ttcgtcgggc ggcagcgtga acgcgccggc atcggctgcg cccggcgacg
     1261 tgaagctcac ggctgcgcag atgcagcatg tgcagttgta taccgtggcc gaagcgcaat
     1321 tccacaaggc gacccgcgcc aacggcacgg tcgatttcga tcaggagcgc tccaccagcg
     1381 tgctcgcgcc gatttccggc ccagtcacga aactcctggt cgcaccgggc gaccgagtga
     1441 agaaagg
//
LOCUS       DASRVO010000161         1311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_56565, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000161 DASRVO010000000
VERSION     DASRVO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56565"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..613)
                     /locus_tag="VFF05_01880"
                     /pseudo
     CDS             complement(1..613)
                     /locus_tag="VFF05_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611481.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(880..>1311)
                     /locus_tag="VFF05_01885"
     CDS             complement(880..>1311)
                     /locus_tag="VFF05_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50586.1"
                     /translation="ALYPDAPAERGGVTVTMSAPESEGTTGVVAQVFTLITGAAAANG
                     FHGIGGRFARDGLLAYAGGDTHVVARLRRRDTGAEVGVALDASRVAPAPDLRERMGRA
                     LAGDATAPEREAFAAAWQDRVRRLLLEHADDPHVIRVERYV"
BASE COUNT          220 a          461 c          391 g          239 t
ORIGIN      
        1 tcgtagctcc acaccgcatt gggccgcacc gcgccgggca accgaatatc gctgccggat
       61 cgccgccgcc gcggccggcg ccggggaatg ttcagcccca gcgtgcgcca caaacggcgt
      121 acgcgcgatt cgctaagcga caaccaggcc gccatgcgcc gataaccgaa gcgcggcacg
      181 tcttgcgagg ctgtaatcag acgctggccc atcacgcgat ccttgccagg ctgcttcaag
      241 gcatagcacg tcacccggcg gctcaggccc aggtagcggc atgccttgcg ttgcgagatt
      301 ccccggcgag tcagatgttc cagcgccacg cgccgtcccg acggggttgt catttttttc
      361 gaccgaactc cttcagtcca tcgatcacca gcatctgttc ggcaaccagg cgcttgagcc
      421 gcgagttttc cgactccagg tccttcagcc gacgggcatc ggccacatcc atgccgccat
      481 acttgttgcg ccaccgatac agggtctgct cggtgatgtt gtgctgcttg cacacctcac
      541 ggatcggaat ctggttcttc tccgcttccc gcaggatccg gatgatctgc tcttcgctgt
      601 gtcgcttctt catggagttc tccttgacca ataggttaag agaactcacc atccaagtgg
      661 ctacacgatt cgtctcaggt cagcccaagg ggcgaatccc gtcagcagga tttaccggac
      721 cgcaaccgat ttgcgctgcg attcgaagag ttccgatccg cgaattgaaa ccgccgcgcg
      781 gagtcgacct gtcccttgcc tcgaatcgtc atcgtgtgga aggcccatca ttgtttcgga
      841 ccgattcatc cggcggccgg cccgtagcta gaactttgct caaacgtagc gctcgacccg
      901 gatcacgtgc gggtcgtcgg cgtgctccag cagcaggcgc cgcacgcgat cctgccaggc
      961 tgcggcgaag gcctcgcgtt cgggcgccgt cgcatcgcct gccagcgcgc gtcccatgcg
     1021 ctcgcgcaga tccggcgccg gtgcaacgcg cgacgcgtcc agcgccacgc cgacctcggc
     1081 accggtatcg cggcgccgca aacgcgcgac cacgtgcgtg tcgccgccag cgtaagcgag
     1141 caggccgtcg cgcgcgaaac gcccgccgat gccgtggaat ccattcgccg cggccgcgcc
     1201 ggtgatcagg gtgaatacct gcgcgacgac gccggtggtg ccctcactct cgggtgcgct
     1261 catcgtaacc gtcacgccgc cgcgttcggc cggagcgtcc ggatacagcg c
//
LOCUS       DASRVO010000162         2361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_290628, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000162 DASRVO010000000
VERSION     DASRVO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290628"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFF05_01890"
     CDS             complement(<1..480)
                     /locus_tag="VFF05_01890"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HZW50587.1"
                     /translation="MLGAIFDRGEAAMRWFSILFLVVGLGLLAGAGFLWRNHAGFAAH
                     AVRADGVVIDLSYHRGSDSSSYYPEVEFKAADGSVVHITGSTGSNPAAYDRGEHVAVM
                     YQPNNPQGARIDSFSENWLGVLILGGMGLVFALIGGGVIYYGMHTRKVNRWLATNGMR
                     "
     gene            583..1854
                     /locus_tag="VFF05_01895"
     CDS             583..1854
                     /locus_tag="VFF05_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW50588.1"
                     /translation="MAHLPADSTTRMGVMDQSDLLKELRIDSRQREDAGGASRWPWIA
                     GAIVLAIALGLGAAWFLFGGKTFEVQAATATAPAAGGGDTAVLQATGYVTARREATVS
                     AQITGTLTQVLIEEGEHVDKDQVLAHLDDTAQKAMLAQAQAQASAAQATLGQYQAQLA
                     QAQRDLKRAQDLIGRHLVSQQALEDAQTQVSALTAQVDAQRKQIELAQAGVKAAQVQL
                     DYCTVRAPFAGVVIAKNAQVGEIVSPFSAGGGFTRTGVGTIVDMDSLEVDVDVNEAYI
                     NRVQRNQPAQAVLDAYPSWTIPAHVIAVIPTADKSKATVKVRIALDQKDARVLPDMGA
                     RVSFLEEAKKNTAAAPLKGVLVPSSSIVQRDGKNFVFAIDGSRARAREVVPGQAYSDL
                     RLVEGIAAGTQVVKQPPAQMTDGAAVKLGNK"
     gene            1881..>2361
                     /locus_tag="VFF05_01900"
     CDS             1881..>2361
                     /locus_tag="VFF05_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZW50589.1"
                     /translation="MGNLVEIRNVSKVYERGKQKVEVLHHVDLDIAQGDFLALMGPSG
                     SGKTTLLNLIGGLDSPSDGSIAVGGSRIDQLGQGALAKWRAANVGFVFQFYNLLPMLS
                     AQKNVELPLLLTKLSAAQRKKNASVALQLVGLADRASHKPTELSGGQQQRVAIARAIV
                     "
BASE COUNT          456 a          727 c          786 g          392 t
ORIGIN      
        1 gcgcatgccg ttcgtggcca gccagcgatt gaccttgcgt gtgtgcatcc cgtagtagat
       61 gacgccgcca ccgatgagtg cgaacaccag gcccatgccg ccaaggatca ggacgccgag
      121 ccagttctcg gaaaaactgt cgatgcgcgc gccttgcgga ttgttcggtt gatacatcac
      181 cgcgacgtgc tcgccgcggt cgtacgcagc cggattcgaa cccgtgctgc cggtgatgtg
      241 cacgacgctt ccgtccgcgg ccttgaattc cacttccggg taatagctgc tgctatcgga
      301 accacggtgg tagctcaggt cgatgaccac gccgtccgcc cgcaccgcgt gtgcggcgaa
      361 gccggcatga ttgcgccaca ggaatccggc gccagcgagc aggccaaggc caacgacgag
      421 aaaaagtata ctgaaccagc gcatggccgc ctccccgcgg tcgaatattg cgccaagcat
      481 gcgggttttg cgcaagtgac gcaaacaccc gtcgatctga caattgtcat ggccaaatcg
      541 tgaccgccga ccctgttgcg gcgcagcgcc ggttgcgaac ctatggcaca cttgccagcc
      601 gactcgacga cacgcatggg cgtcatggat caatccgatt tgttgaagga attgcgcatc
      661 gattcgcgcc agcgcgagga tgcgggtggc gcctcgcgct ggccgtggat cgccggcgcg
      721 atcgtgctgg ccattgcgct tggcctcggc gcggcctggt tcctgttcgg cggcaagacg
      781 ttcgaggtgc aggcggccac ggcgaccgcg ccggcagccg gcggcggcga cacggccgtg
      841 ttgcaggcga cgggttacgt caccgcgcgg cgcgaggcca cggtttccgc gcagatcacc
      901 ggtaccctga cgcaagtgct gatcgaggaa ggcgagcacg tcgacaagga tcaagtgctg
      961 gcgcatctgg acgataccgc gcaaaaagcg atgctcgcgc aggcacaggc gcaggccagt
     1021 gcggcgcagg cgacgttggg ccaatatcag gcgcaattgg cgcaggcgca gcgcgatctc
     1081 aagcgcgccc aggatctgat cggtcggcac ttggtctcgc agcaggcact ggaagatgcg
     1141 caaacgcaag tatccgcgct gaccgcgcaa gtcgatgcac aacgcaagca gatcgaactg
     1201 gcgcaggccg gcgtcaaggc tgcgcaggtg caactggatt actgcacggt gcgcgcgccg
     1261 ttcgccggcg tggtcatcgc caagaacgca caggtcggcg aaatcgtctc gccgttttcc
     1321 gccggcggcg gttttacccg caccggcgtc ggcaccatcg tcgacatgga ttcgctggaa
     1381 gtcgacgtcg atgtcaacga ggcctacatc aatcgcgtgc aacgcaacca gcccgcgcag
     1441 gcggtgctcg atgcgtatcc gagctggacg attcccgcgc acgtgatcgc ggtgattccg
     1501 accgcagaca agagcaaggc cacggtcaag gtgcgcatcg cgctcgatca gaaggatgcg
     1561 cgcgtgctgc ccgacatggg cgcgcgcgtg tcgttcctgg aagaagcgaa gaagaacacg
     1621 gcggcggcgc cgctcaaggg cgtgctggtg ccgtcatcaa gcatcgtgca gcgcgacggc
     1681 aagaattttg tgttcgcgat cgatggcagt cgcgcgcgtg cgcgcgaagt ggtgccgggg
     1741 caggcgtatt cggacctgcg tctcgtcgaa ggtatagcgg cgggcacgca ggtggtcaag
     1801 cagccaccgg cgcagatgac cgatggcgcg gcggtgaaat tgggcaacaa ataactacgg
     1861 gatttttcga ggatcggaca atgggcaatc tggtggaaat ccgcaatgtc agcaaagtct
     1921 atgaacgcgg caaacagaag gtcgaggtgc tgcaccacgt cgatctcgac atcgcacagg
     1981 gcgatttcct cgccttgatg gggccatcgg gttccggcaa gacgaccttg ctcaatctca
     2041 ttggcggcct ggattcgccc agcgacggca gcatcgccgt cggcggctct cgcatcgacc
     2101 agctcggcca gggcgcgctg gcgaaatggc gcgcggcgaa cgtcggcttc gtgttccagt
     2161 tctacaattt gttgccgatg ctgagtgcgc agaagaacgt ggaactgccg ctgctgctga
     2221 ccaagctttc cgccgcgcag cgcaagaaga atgcgagcgt cgcgctgcaa ctggtggggc
     2281 ttgccgaccg cgcctcgcac aagccgaccg aactctccgg cggccagcag cagcgcgtgg
     2341 cgatcgcacg cgcgatcgtg t
//
LOCUS       DASRVO010000163         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_82760, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000163 DASRVO010000000
VERSION     DASRVO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82760"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFF05_01905"
     CDS             <1..550
                     /locus_tag="VFF05_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HZW50590.1"
                     /translation="DDGATWKQSAQGMRAAYMPAERAYEPNAQDVHHMVQCRGQPDHF
                     WAQHHNGVFRNNGGDWQEIENVQPSVFGFAVAVHPRDANTAWFVPATKDERRIPVDGK
                     VVVARTRDGGKSFDVLRNGLPQNDAYDLVLRHALAIDGSGERLAFGSSTGGVWISEDQ
                     GDRWAMLDARLPQVYAVTFANA"
     gene            618..815
                     /locus_tag="VFF05_01910"
     CDS             618..815
                     /locus_tag="VFF05_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50591.1"
                     /translation="MPSNPHPKSSQVVPRQRAPVDVFQHVHQVRLRERRLDDTIDQSF
                     PASDPPGWTLGPNWRGAAPKP"
     gene            920..1420
                     /locus_tag="VFF05_01915"
     CDS             920..1420
                     /locus_tag="VFF05_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW50592.1"
                     /translation="MANFELFKRIFGNSQERREKPRVNAREGTVVLIVDDSPTIVAML
                     RKFLQQNGYQTLEAGDAEKGIEVARAHRPHLIFLDIVLPGMNGFAALRALRKDPVTLG
                     IPIVMISGNEQATEQFYAQRIGADDFMKKPFSRSEVFARIERLLDVDMVPKRPAPPPI
                     APVPRA"
     gene            1450..2118
                     /locus_tag="VFF05_01920"
     CDS             1450..2118
                     /locus_tag="VFF05_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZW50593.1"
                     /translation="MAVLLAGCAQGIRPPETAAPGQASPSTPTYETVQGRSPDVVARL
                     RAAPPVQAEIVDGATPAGDETLLRAQGLVKIGIGHYPVADVARLRDVVSRQAREVGAD
                     KAMIYPPPAFGDPALAQFFVRLHLPFGANFRDLTDDERQSLGKGGVEIGEVVGGTPAS
                     EANLRDGDYVLQFNKTPVQDKAAFQVLLQEHMGRRVTLTIRRNGTTMKRLVVLGTLPA
                     PPAH"
     gene            2188..>3713
                     /locus_tag="VFF05_01925"
     CDS             2188..>3713
                     /locus_tag="VFF05_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HZW50594.1"
                     /translation="MNRVPFRPLLAALSMSICVCAAADGPAPADTPLLGFTVDGSSAQ
                     RALEARFDAQLSASEMREWLKHLAAEPNQVVSPHDKANAQFMLSLFKQWGWDARIESF
                     DVLYPTPKEVSVELIAPTQFKATLHEPPVAGDATSGLSGALPPYNVYGADGDVTADLV
                     YVNYGMPDDYKELARHGVDVKGKIVIVRYGGGWRGLKPELAYQHGAVGCLIYSDPRDD
                     GYFAGDTYPKGGWRPSDGVQRGSVAKMQLYPGDPTTPGYGSVPGAKHVAAKDAQTVLK
                     IPVLPISYADATPLLQALGGPVAPEGWRGALPLTYHLGAGPAKVHLVVKSDWNLKPIY
                     DVIATIKGAQEPDRWIVRGNHHDGWVFGAWDPLAGMIAELGEAKAIGALVKSGWKPKR
                     TIVYASWDGGEPGLLGSTEWAETHAKELQRKAVMYLNSDTNGRGFLGAEGSHSLQHLL
                     NAAAADVTDPEKAVSVLERRRAEMRVAGARPDAKPEAKENAKLAAAGGDLPIGALGSG
                     S"
BASE COUNT          658 a         1225 c         1229 g          601 t
ORIGIN      
        1 tgacgatggc gcaacgtgga agcaaagcgc acagggcatg cgtgcggcct acatgccggc
       61 cgaacgcgcc tacgagccga acgcacagga cgttcaccac atggtgcagt gccgcggcca
      121 gcccgatcat ttctgggcgc agcaccacaa cggcgtgttc cgcaacaacg gcggcgactg
      181 gcaggaaatc gaaaacgtgc agccgtccgt gttcggtttc gccgtggccg tgcatccgcg
      241 cgatgcgaac acggcgtggt tcgtgccggc gaccaaggac gaacgccgca ttccggtcga
      301 cggaaaagtg gtggtcgcgc gcacgcgcga tggcggcaaa tccttcgacg tgttgcgcaa
      361 tggcttgccg cagaacgatg cctatgatct cgtcctgcgt cacgcgctcg cgatcgacgg
      421 cagcggcgaa cgcctcgcct ttggctcgtc gaccggcggc gtgtggatca gcgaggatca
      481 gggcgaccgc tgggccatgc tcgacgcgcg cctgccgcag gtttacgcag tgacgttcgc
      541 caacgcctga cctgatgcag gtcaagtctg cggaattttg cggacgttac actgccggtg
      601 ggtattggga gaacgccatg ccatcgaatc cccatccgaa atcttcgcag gtcgttccgc
      661 gccagcgcgc gcccgtggac gttttccagc acgtccatca agtgcgtctt cgcgagcggc
      721 gcctcgacga caccatcgac cagagcttcc cggccagcga tccgcccggc tggacgctcg
      781 gacccaattg gcgcggcgcc gcgcccaagc cctagcaggc gaacccgcac gccggttcct
      841 ggccggcagc cacgaaaatg cttgccagca ttcgcggcaa ccgcgaaact tgcacgagtt
      901 tcggcacagg aaccggcccg tggccaattt cgagttgttc aagcgcatct tcggcaacag
      961 tcaggagcgc cgcgaaaagc cgcgcgtcaa cgcgcgtgaa ggcacggtcg tcctcatcgt
     1021 cgacgattcg ccgaccatcg tggcgatgct gcgcaagttc ctgcagcaaa acggctacca
     1081 gacgctggaa gccggcgatg cggaaaaagg catcgaggtt gcgcgtgcgc atcgcccgca
     1141 cctgatcttc ctcgacatcg tcctgcccgg catgaacggt ttcgccgcgc tgcgcgcctt
     1201 gcgcaaggat ccggtcacgc tcggcattcc catcgtgatg atcagcggca acgagcaggc
     1261 gaccgagcag ttttacgcgc agcgcatcgg cgccgacgat ttcatgaaga agccattttc
     1321 gcgatccgaa gtgttcgcgc gcatcgagcg cctgctcgat gtggacatgg ttcccaagcg
     1381 tcccgcgccg ccgccgatag cgccggtacc gcgagcgtaa gcatgcgcgt tcgcagcgcc
     1441 gcgtgctgga tggcggttct gttggccggc tgcgcgcagg gcatcaggcc gccggagact
     1501 gcagcacctg ggcaagcgtc gccatcgacg ccgacctatg aaaccgtgca gggccgcagt
     1561 cccgatgtcg tcgcgcgtct gcgtgccgcg ccgccggtac aagccgagat cgtcgacggc
     1621 gccacgcccg ctggcgacga gacgctgctg cgcgcgcaag gactggtgaa gatcggcatc
     1681 ggccactatc cggtcgcaga tgtggcgcga ttgcgcgatg tagtgtcgcg gcaggcgcgt
     1741 gaggtgggcg cggacaaggc catgatctat ccgccgccag cgttcgggga tcccgcgctg
     1801 gcgcaattct tcgtgcgcct gcacctgccg ttcggcgcga atttccgcga cctcaccgac
     1861 gacgaacggc agtcgctcgg caagggcggc gtggagatcg gcgaggtcgt cggcggcacg
     1921 ccggcatccg aggcaaacct gcgcgatggc gattacgtcc tgcaattcaa caagacgccg
     1981 gtacaggaca aggccgcgtt ccaggtgctg ctgcaggaac acatgggccg gcgcgtcacc
     2041 ctgaccatcc gccgcaacgg cacgacgatg aagcggctgg tcgtgctcgg cacgctgccg
     2101 gcgccgcccg cgcactgacc atcgcccctt gacgcccgcg ccgtcgaggt tctcaatcgc
     2161 ggttcgtcca cgcaacaggg aattgccatg aaccgtgttc cgttccggcc gctgctcgcg
     2221 gccttgtcga tgtcgatttg cgtctgcgcc gcggcggatg gtcccgcgcc ggcggacacg
     2281 ccgctgctcg gcttcactgt cgatggctca tccgcgcagc gtgcgctcga ggcgcgcttc
     2341 gacgcgcaac tgagcgcgag cgaaatgcgc gagtggctca aacatctggc ggccgagccc
     2401 aatcaggtcg tttcgccgca cgacaaggcc aatgcgcagt tcatgctgtc gctgttcaag
     2461 cagtggggct gggacgcgcg catcgagtcc ttcgacgtgc tttatccgac gccgaaggaa
     2521 gtgtcggtcg aactcatcgc gccgacgcag ttcaaggcga cgttgcacga accgccggtc
     2581 gccggggatg cgacgtcggg actgtccggc gcgctgccgc catacaacgt ctacggcgcc
     2641 gacggcgacg tcacggccga tcttgtctac gtcaattacg gcatgcctga tgattacaag
     2701 gagctcgcac gccacggcgt cgacgtcaaa ggcaagatcg tcatcgtgcg ttacggcggc
     2761 ggctggcgcg gactcaaacc cgaacttgcc taccaacacg gcgcggtcgg ctgcctcatc
     2821 tattccgatc cacgcgacga cggctatttt gccggcgaca cctatccgaa aggcggctgg
     2881 cgtccgtccg acggcgtgca gcgcggctcc gtcgcgaaga tgcagttgta tccgggcgat
     2941 ccgaccacgc ccggctacgg ttcggtaccc ggggcgaaac atgtcgcggc caaggatgcg
     3001 cagaccgtcc tgaaaattcc ggtgttgccg atctcctacg ccgacgcgac gccgctgctg
     3061 caggcgctcg gcggcccggt cgcgccggaa ggctggcgcg gcgcgctgcc gctcacctat
     3121 cacctcggcg cgggcccggc gaaggtgcat ctggtggtga aatcggattg gaatctgaaa
     3181 ccaatctacg acgtgatcgc gacgatcaag ggcgcgcagg aaccggatcg ctggatcgtg
     3241 cgcggcaacc atcacgacgg ctgggtgttc ggcgcgtggg atcctctggc cggcatgatc
     3301 gccgagctcg gcgaggccaa ggcgatcggt gcgctggtca aatccggctg gaagccgaaa
     3361 cgcaccatcg tttacgccag ttgggatggc ggggagccgg gcctgctcgg ctccaccgag
     3421 tgggccgaaa cgcatgccaa ggaattgcag cgcaaggccg tgatgtatct caactcggat
     3481 accaacgggc gcggattcct cggcgccgag ggcagccatt cgttgcagca cttgttgaac
     3541 gcggcagccg ccgacgtgac cgatccggaa aaggccgtca gtgtgctgga gcgccggcgc
     3601 gccgagatgc gcgtggccgg cgcacggccc gacgcgaaac ccgaagccaa ggaaaacgca
     3661 aagctcgcgg cggctggcgg cgacctgccg atcggtgcgc tgggctccgg ttc
//
LOCUS       DASRVO010000164         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_108463, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000164 DASRVO010000000
VERSION     DASRVO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108463"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFF05_01930"
     CDS             complement(<1..651)
                     /locus_tag="VFF05_01930"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZW50595.1"
                     /translation="MILWEASPCAPIAARVAVAGDFLPDWKPGAARPNVPAGSGWKEL
                     AQKLAPHFEDVAISFANCECTLDTARLAPHPVGGLGENISVPAGTLDYLAAIRARVVG
                     VANNHVYDFGGEGVGRTRRAIAARGLTPLGAGRTLDAAPEVFVWRGPEPLRVGFWAAA
                     AATRTPSTRAFAGVEPATLSRARQALAAMDDQGARFRIALLHAGCLRASYPAPEDLR"
     gene            complement(680..>1236)
                     /locus_tag="VFF05_01935"
     CDS             complement(680..>1236)
                     /locus_tag="VFF05_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50596.1"
                     /translation="AFVLLVAISPVVSVMWLGRYQPLFVEFVALLAAGWLVNVLSNPA
                     YVVDLGTGALRWVSRGCLLTAALNAGLGFAAGKMFGGPAIVLAAVVSLISGYAVILGA
                     YHVQNRVPFRELIPPASAGILAASGGVALLIFPFVRASHAGSFSAARVAWGLTVALVM
                     LIVPMWTHPMRRRVWNWVTSGAAA"
BASE COUNT          253 a          431 c          396 g          156 t
ORIGIN      
        1 ccgcaggtct tccggagcag gatagctggc gcgcaagcat ccggcgtgca gcagcgcgat
       61 gcggaagcgc gcaccctggt cgtccatcgc tgcgagcgcc tggcgggcgc gcgacaatgt
      121 ggcgggttcg actccggcga acgcgcgcgt cgacggcgta cgcgtcgctg ccgctgcggc
      181 ccagaatccg acgcgaagcg gctccggccc gcgccagacg aacacttccg gcgccgcgtc
      241 gagagtgcgc ccggcgccga gcggtgtgag cccgcgagcg gcgattgcgc ggcgcgtgcg
      301 tccgacgcct tcgccgccga aatcgtagac atgattgttc gcaacgccaa ccacgcgtgc
      361 gcgaatcgcc gcaaggtaat cgagagtgcc cgcgggaacg gaaatgttct cgcccagccc
      421 gccgaccggg tgaggagcaa gacgcgcggt atccagcgtg cattcgcaat tcgcaaacga
      481 aatcgccaca tcctcgaaat gcggggcgag cttttgcgcc agttctttcc agcccgagcc
      541 cgccggaaca ttcggtcgtg cagcgcccgg tttccagtcg ggcaggaaat ctccggcgac
      601 cgcgacgcga gcggcgattg gcgcgcacgg gctcgcttcc caaagaatca tcgtcgaaag
      661 gactccccca gcgaataatt cacgccgctg ctccggaagt gacccaattc caaacccgcc
      721 gacgcattgg atgggtccac atcggcacga tcagcatcac cagcgccacg gtgagtcccc
      781 atgcgacgcg cgcagctgaa aacgagccgg cgtggctcgc acggacaaac ggaaagatca
      841 ggagcgcaac accgccgctg gccgccagga tgcccgcact cgcgggcggg atcagctccc
      901 ggaacggcac gcgattctga acgtggtacg cgccgagaat caccgcgtag cccgagatca
      961 gggaaacgac ggccgcgaga acgattgcag ggccgccaaa cattttgccc gctgcgaagc
     1021 ccaggcccgc attcagcgcc gctgtgagca gacatccccg cgaaacccag cgcagcgcgc
     1081 ccgtgccgag atcgaccaca taggccggat tgctgagcac gttgaccagc cagcccgccg
     1141 ccagcagcgc gacgaattcg acgaacagcg gctggtagcg gcccagccac atcaccgaaa
     1201 caaccgggct gatcgccacc agcagaacga acgcgg
//
LOCUS       DASRVO010000165         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_238008, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000165 DASRVO010000000
VERSION     DASRVO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238008"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..>1091
                     /locus_tag="VFF05_01940"
     CDS             225..>1091
                     /locus_tag="VFF05_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HZW50597.1"
                     /translation="MSLRRGLPVLFCCIAALIAGCAEIGAPRQETPDAVAADQAYGQG
                     NFDAAARDFLALADAHPRDGAFYRLRAAEAYLQNGEIDAAARTLPDIQRRHLQGDEPI
                     RYDLLAAEVALSRHDAAGALAALHFSDANLATPLRLRTLELRARAQIAHGDALDSART
                     RADLDRLLGGNDRAQNEAQIVATLKTMSQDALRTEAAMLVPGDPLQPWIDQALHASGG
                     ALPVVVLHPNQPVGTLIPQGTQPPQREGYAQPHRIALLLPESGPLMAVAQPVRDGFFA
                     AYFADDSAQRAPI"
BASE COUNT          182 a          387 c          354 g          168 t
ORIGIN      
        1 cgggcagtct ttgcgttcat cgcatccaac tatgtggaac ctttgaaaaa ccgtcgcgag
       61 cgaagacagg tcacgcgccg ccagcgcgca ggaaccggag tttactcgga tgtaaatgag
      121 gattccgagc actgccggcg cgagagatgt cgagcgcagc aggtttttca gaggttccag
      181 gcgcgatccg ctatcatgcg agctcattcg tcgggaatgc ggccatgagt ttgcgacgcg
      241 ggttgcctgt cttgttctgc tgcatcgcgg ccctcatcgc cggttgcgcg gaaatcggcg
      301 ccccgcgcca ggagacgccc gatgccgtcg ccgcggacca ggcctacggc cagggcaatt
      361 tcgacgcagc cgcgcgcgat ttcctggcgc tcgccgatgc gcatccgcgc gacggcgcgt
      421 tttaccggct gcgcgccgcc gaggcgtatc tgcagaacgg cgaaatcgat gcagcggcac
      481 gcacgctgcc ggacatccag cgccgccacc tgcagggcga cgagcccatc cgctacgacc
      541 tgcttgccgc cgaagtcgca ctgtcgcggc atgacgcggc gggcgccctg gccgccctgc
      601 atttttccga tgcgaacctg gcgacaccgc tgcgcctgcg cacgctggaa ttgcgcgccc
      661 gtgcccagat cgcccacggc gatgcgctgg acagcgcacg cacgcgcgcc gatctcgatc
      721 gcctgctcgg cggcaacgac cgcgcgcaaa acgaggcgca gatcgtcgcc acgctcaaga
      781 ccatgagtca ggatgcgctg cgcaccgaag cggcgatgct ggtgccgggc gatccgctgc
      841 agccgtggat cgatcaggcg ctgcacgcca gcggcggcgc cctgccggtc gtcgtgctgc
      901 atccgaacca gccggtcggc acgctgatcc cgcaaggtac gcaaccgccg cagcgcgaag
      961 gttacgcgca gccacaccgt atcgcattgc tgctgcccga gagcggcccg ctgatggccg
     1021 tggcgcagcc ggtgcgcgac ggcttcttcg ccgcctactt cgccgacgac agcgcacaac
     1081 gcgcccccat c
//
LOCUS       DASRVO010000166         5494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_4748, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000166 DASRVO010000000
VERSION     DASRVO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4748"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFF05_01945"
     CDS             complement(<1..881)
                     /locus_tag="VFF05_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZW50598.1"
                     /translation="MPIVDQVLEARWVVPVEPHGVVLEDHAVALAGDAIAEVLPIAAA
                     RAKYANVERTRLPEHILIPGLVNAHTHNPMTLMRGLADDLPLMIWLQQHIWPAEAKSV
                     GPEFVRDGVELAIAEMLRGGTTCCNESYFFPDVQAATYLRHGFRAVVGLPFIEFPTAW
                     AKSRDEYFDKNLAVHDEFKGEKLVSTSLAPHAPYTVADESFARIRMLSDQLDIPVHLH
                     LHETAQEIEDSRKQHGQRPFARMQALGLVDERLIAVHMTQLTDAEIAACAQHGVSVVH
                     CPESNLKLASGFCPAEKL"
     gene            complement(888..1451)
                     /gene="efp"
                     /locus_tag="VFF05_01950"
     CDS             complement(888..1451)
                     /gene="efp"
                     /locus_tag="VFF05_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZW50599.1"
                     /translation="MAYGMNDVKNGMKILVDGYPCTIVDTEYVKPGKGQAFTRVKYRN
                     LKTGRVVELTMKSTDSLEAADVMDQDMEYLYSDGEFWHFMHPETHEQIAADKNAMVDA
                     EKWLKGNEECIVTLWNGSPLTVAPPNFVELVITETDPGVKGDTAGTGGKPATLETGAV
                     VRVPLFVPQGEKIKVDTRTGEYVGRVK"
     gene            1528..2535
                     /gene="epmB"
                     /locus_tag="VFF05_01955"
     CDS             1528..2535
                     /gene="epmB"
                     /locus_tag="VFF05_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HZW50600.1"
                     /translation="MITASPLPAQQPRTWQWLWREAIADPRELLELLELGHLAATLLP
                     ARDTGFAMRVPRGFAARMRRGDPADPVLLQVLPQAAELLEAPGFGADAVGDMSARVGG
                     GVLHKYAGRALLIATGSCAVNCRYCFRRHFPYAQETAAANRWQEAIERIHDDASIEEV
                     ILSGGDPLSLATAKLAKLTTELASIAHLRRLRIHTRLPVVLPERVDAELLEWLRRLPW
                     PVAIVLHANHANEIDASVADACARLRGAGTSLLNQSVLLRGVNDSVDALADLSARLFA
                     AGVLPYYLHQLDRVAGAAHFAVADERARELIVRLRGRLSGYLVPRLVREIAGDPAKAP
                     L"
     gene            2600..4672
                     /locus_tag="VFF05_01960"
     CDS             2600..4672
                     /locus_tag="VFF05_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW50601.1"
                     /translation="MSKQENVIKLLLIEDSVEEAEQLISMLRNGGIAVRPARAGNADE
                     LKAQLTAQTPDLLLVNVAARGVTLKDAAAATNATGKDVAIVAVAATTNEDTVANAFTD
                     GARALTLRNRAEHVQSIVRREFEALLMRRSVRRLETALRETERRCEALLDSSRDPIAY
                     VHEGMHVRANKAYLEIFGFEEFEDIEGMSILDMIAPDDAEDFKALLKKLSKGEKPPQK
                     LNLKAQRADGSTFDATLEFAEASYEGEPCQQIIFRQQTMSAELAKELDVLRSKDLVTD
                     LYNRQYCLGELDRMVGDAAKGRNDQALLLVEPDNFKALLDTVGLGNADLLLGDMANLM
                     RRHLKDADIAGRFGEHAFGVLVSGRKADEVRQLGETLRHAFEERIFEVGKQSVNANVS
                     IGGTLIGEKNANAQAVLTQASTALRTAQTEGGNRVNVFDPGAKDKADEEKNRHWLTLV
                     KDALANNGFVLFYQPIISLHGAEGEFYEILLRMNGPKGEIGPAFFMPIAEQHGLLSQI
                     DRWVIGAAIKALAEREKAGHKTTFFIKLTPQSLDDQTLLPWIAQQLKNARLRGDALVF
                     EMPESKVVTSLKPARAFVTGLKQIHCGFALEQFGSGLNSFQLLKHVDANYLKLDRNFM
                     AELPKNKEHQEKIKELCDQAHHAGKLTVAEFVEDAASMSILFSCGVNFVQGNFLQEPE
                     KVMAYETA"
     gene            4727..5443
                     /gene="pyrH"
                     /locus_tag="VFF05_01965"
     CDS             4727..5443
                     /gene="pyrH"
                     /locus_tag="VFF05_01965"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZW50602.1"
                     /translation="MPAKIKYKRILLKLSGEALLGKADYGIDPQMIGRLARETIEVQQ
                     AGVEVGIVIGGGNIFRGEGLAAAGMDRVTGDNMGMLATVINALAMQDALEKLGARARV
                     MSAIKINEVCEDFIRRRAIRHLEKGRIAIFAAGTGNPFFTTDSAAALRAIEIDADLLL
                     KATKVDGVYSADPKKDKNAQRFEHLTYDQVIERKLAVMDTAAIALCRDHKIPLRIYDM
                     RREGDLMRIMRGAQIGTLVS"
BASE COUNT         1039 a         1782 c         1703 g          970 t
ORIGIN      
        1 agtttttccg ccgggcagaa tccgctggcg agtttcaggt tggattccgg gcaatgcacc
       61 accgacacgc cgtgctgcgc gcaggcggcg atttcggcat ccgtcagttg cgtcatgtgc
      121 accgcgatca ggcgctcgtc gacgagtccg agcgcctgca tgcgcgcgaa cgggcgctgg
      181 ccgtgctgct tgcgcgaatc ctcgatttcc tgcgcggttt cgtgcaagtg cagatgtacg
      241 ggaatgtcga gctggtccga cagcatgcgg atgcgcgcga agctttcgtc agcgacggtg
      301 tacggcgcat gcggcgcaag cgaagtggaa acgagcttct cgcccttgaa ctcgtcgtgg
      361 acggcgaggt tcttgtcgaa gtattcgtcg cggctcttcg cccacgcggt cgggaattcg
      421 atgaacggca aaccgacgac ggcgcgaaag ccgtggcgca ggtaggtcgc cgcctgcaca
      481 tcgggaaaaa aataactctc gttgcaacag gtagtaccgc cgcgcagcat ctcggcgata
      541 gccagttcca cgccgtcgcg cacgaattcc gggccgacgg atttggcctc ggccggccag
      601 atgtgctgct gcagccagat catcagcggc aggtcgtcgg ccagaccgcg catcagggtc
      661 atggggttgt gcgtgtgcgc gttgaccagt cccgggatca gaatgtgttc cggcaagcgc
      721 gtgcgttcga cattcgcata ttttgcgcgc gccgccgcga taggcagcac ctcggctatg
      781 gcgtcgcccg ccagtgcgac ggcgtgatct tccagcacca cgccgtgcgg ttcgaccggg
      841 acgacccagc gcgcttcgag aacctgatcg acgattggca tcccggttta ctttacgcgc
      901 cccacgtact cgcccgtgcg cgtatccacc ttgatcttct cgccttgcgg cacgaacagc
      961 ggcacgcgta ccacggcgcc ggtttccagc gtcgccggct tgccgccggt gccggcggtg
     1021 tcgcccttga cgcccggatc ggtttcggtg atgacgagtt ccacgaagtt cggcggcgcc
     1081 acggtcagcg gcgagccgtt ccacaacgtg acgatgcatt cctcgttgcc cttgagccac
     1141 ttttccgcgt cgaccatcgc gtttttgtcc gcggcgatct gctcgtgcgt ctccggatgc
     1201 atgaagtgcc agaactcgcc atcgctgtac aggtattcca tgtcctggtc catgacatcg
     1261 gccgcttcca gcgaatcggt ggatttcatg gtcagttcga ccacgcgccc ggtcttcagg
     1321 ttgcggtact tgacgcgggt gaaggcctga cctttgccgg gcttgacgta ctcggtgtcg
     1381 acgatcgtgc acggatagcc gtccacgaga atcttcatgc cgttcttgac gtcgttcatg
     1441 ccgtaagcca tgggtaaaac tccgttgaat gtcggggacc gttgggaaaa cggctaaaat
     1501 gggcagattc gggaaatccg gccgtccatg ataaccgcaa gccccctgcc cgcgcagcag
     1561 ccccgcacct ggcaatggct gtggcgcgag gcgattgccg atccgcgcga actgctcgaa
     1621 ctgctcgaac tcgggcatct ggccgccacg ttactgcccg cgcgcgacac cggttttgcc
     1681 atgcgtgtac cgcgcgggtt cgccgcgcgc atgcgccgcg gcgatccggc cgatccggtg
     1741 ttgctgcaag tgctgccgca ggcggccgag ttgctcgaag cgccgggttt cggtgcggat
     1801 gccgtcggcg acatgagcgc gcgcgtgggc ggcggcgtgc tgcacaaata cgcgggccgc
     1861 gcgctgctga tcgccacggg ttcgtgtgcg gtgaactgcc gctactgctt ccgccggcat
     1921 tttccctacg cgcaggaaac cgcggcggcg aatcgctggc aggaagccat cgaacgcatt
     1981 cacgacgatg catcgatcga agaagtgatt ctttccggcg gcgatccgct ttcgctggca
     2041 acggccaagc ttgccaaact caccacggaa ctcgcctcga ttgcgcatct gcgacggctg
     2101 cgcatccaca cgcgtttgcc tgtcgttctg cccgagcgcg tcgacgccga attactcgaa
     2161 tggctgcgcc gcctgccctg gcctgttgcc atcgtgctgc acgccaatca cgccaacgag
     2221 atcgatgcgt cggtcgccga tgcctgcgcg cggctgcgcg gcgcgggtac ttcgctgctc
     2281 aatcaatccg tgctgctgcg cggcgtgaac gacagcgtcg acgcgctggc ggatctgtcc
     2341 gcacgcctgt tcgcggccgg cgtgctgccc tactacctgc accagctcga ccgcgtcgcc
     2401 ggcgcggcgc attttgcggt ggctgacgag agagcacgcg agttgatcgt gcggctgcgc
     2461 gggcgtctat ccggctacct ggtgccgcgc ctggtgcgcg agattgcggg cgatccggcg
     2521 aaggccccat tatgactgtt ggcgctggaa aagccgccgt taatcgagta aaagctaagc
     2581 cgcacgggaa gcgcgagcga tgagcaaaca ggaaaacgtc atcaagctgc tcttgatcga
     2641 ggattcggtc gaggaagccg agcaattgat cagcatgctg cgcaacggcg gcatcgccgt
     2701 gcgtcctgcg cgcgcgggca acgccgacga actcaaggct cagttgaccg cgcaaacgcc
     2761 cgatctgctg ttggtcaacg tggccgcgcg cggcgtcacg ctcaaggacg ccgccgccgc
     2821 cacgaacgcc accggcaagg atgtcgccat cgtcgccgtc gccgccacga ccaatgaaga
     2881 tacggtcgcc aacgcattca ccgacggcgc gcgcgcgctg accttgcgca accgcgccga
     2941 gcacgtgcaa tccatcgtgc gccgcgaatt cgaggccctg ctgatgcgcc gcagcgtgcg
     3001 ccgcctcgag acggcgctgc gcgaaaccga gcgccgctgc gaggcgctgc tggattcctc
     3061 gcgcgatccc atcgcctacg tgcacgaagg catgcatgtg cgcgcaaaca aggcctacct
     3121 cgaaatcttc ggcttcgagg aattcgagga tatcgaaggc atgtcgatcc tggacatgat
     3181 cgcgccggac gatgccgagg acttcaaggc cctgctcaag aaactctcca agggcgagaa
     3241 accgccgcaa aaactcaatc tgaaggcgca acgtgccgac ggcagcacgt tcgacgcgac
     3301 cctggaattt gccgaagcca gttacgaggg tgaaccctgc cagcagatca tcttccgcca
     3361 gcagacgatg agcgcggaac tggccaagga acttgacgta ttgcgttcca aggacctggt
     3421 cactgacctg tacaaccgcc agtattgcct cggcgaactc gatcgcatgg tcggcgacgc
     3481 tgccaagggc cgcaacgacc aggctctgct ccttgtcgag ccggacaact tcaaggccct
     3541 gctcgatacg gtcggcctcg gcaacgcgga tcttctgctc ggcgacatgg ccaacctgat
     3601 gcgccggcac ctgaaggatg ccgacatcgc cggccgtttc ggcgaacacg cgttcggcgt
     3661 cctcgtctcc ggcagaaagg cggatgaggt tcgccaactc ggcgaaacgc tgcgccatgc
     3721 ctttgaagag cgcatcttcg aggtcggcaa acagtcggtg aacgcgaacg tcagcatcgg
     3781 cggcacgttg atcggcgaga agaatgccaa cgcccaggct gtgctcacgc aggcaagcac
     3841 cgcgctgcgc acggcgcaga ccgagggcgg caaccgcgtc aacgtgttcg atcctggagc
     3901 caaggacaag gccgatgagg agaaaaaccg gcattggctt accctggtca aggacgctct
     3961 cgccaacaac ggcttcgtgc tgttttacca gccgatcatc agcctgcacg gcgccgaggg
     4021 cgagttctac gaaatcctgc tgcgcatgaa cggacccaag ggcgagatcg ggccggcttt
     4081 cttcatgccc atcgccgaac agcacggctt gctctcccaa atcgaccgct gggtgatcgg
     4141 cgccgcgatc aaggcattgg ccgagcgcga gaaggccggc cacaagacga cgttcttcat
     4201 caaactcaca ccgcagtcgc tggacgacca gaccctgctg ccttggatcg cgcagcagtt
     4261 gaagaacgcg cgcctgcgcg gcgatgcgct ggtattcgag atgccggaaa gcaaggtcgt
     4321 caccagcctc aagccggcgc gcgccttcgt caccggactc aagcagattc actgcggctt
     4381 tgccctggag cagttcgggt cgggcctgaa ttcgttccag ttgctcaagc acgtggacgc
     4441 gaactacctc aagctcgacc gcaacttcat ggccgaactg ccgaaaaaca aggaacacca
     4501 ggaaaagatc aaggaactgt gcgaccaggc gcaccacgcc ggcaagctca ccgtcgccga
     4561 attcgtcgag gacgcggcga gcatgtcgat cctgttttcc tgcggcgtga acttcgtgca
     4621 gggcaatttc ctgcaagagc ccgagaaagt gatggcttac gagaccgcct gacgtctcga
     4681 cactcggcta caatacgcgc gttttgccca tccgccggag ccgcgcatgc ctgccaagat
     4741 caagtacaaa cgcatcctgc tcaagctctc cggcgaagcc ctgctcggca aggccgatta
     4801 cggcatcgac ccgcagatga tcggccgcct ggcccgcgag acaatcgaag tgcagcaagc
     4861 cggcgtcgag gtcggcatcg tcatcggcgg cggcaacatc tttcgcggtg aaggtctggc
     4921 cgcggccggc atggatcgcg tcaccggcga caacatgggc atgctcgcca ctgtcatcaa
     4981 cgcgctggcg atgcaggacg cgctggaaaa actcggtgcc agggcgcgcg tgatgagcgc
     5041 gatcaagatc aacgaagtct gcgaggattt catccgccgc cgcgcgatcc ggcatctgga
     5101 aaaaggccgc atcgcgatct tcgccgccgg caccggcaac ccgttcttca ccaccgattc
     5161 cgccgccgcg ctacgcgcca tcgaaatcga cgccgacctg ctgctcaagg cgaccaaggt
     5221 cgatggcgtg tacagcgccg acccgaaaaa agacaagaac gcgcaacgct tcgagcatct
     5281 cacttacgac caggtgatcg aacgcaagct cgccgtgatg gacaccgctg ccatcgccct
     5341 gtgccgcgac cacaagattc cgctgcgcat ctacgacatg cgccgcgaag gcgatttgat
     5401 gcgcatcatg cgcggtgcgc agatcggcac gctggtaagc tgatttccat tgcggccgta
     5461 ccctgcaccg cttctggcac gcgttcggaa gtcg
//
LOCUS       DASRVO010000167         1240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_342443, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000167 DASRVO010000000
VERSION     DASRVO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342443"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VFF05_01970"
     CDS             <1..1103
                     /locus_tag="VFF05_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW50603.1"
                     /translation="LVLGRQMRETEALAEQRGIDLANLAQINELIIRRMKTGVLVVDD
                     ANRVHRWNESAWSLIGNPKSGQRDLGSVAPELSRRLYHWRTTGKIDATAVALADGVPE
                     VIPRFTRLSSNDDENVLIFLDDTSLVSRRAEELTLSSLGRLSASIAHEIRNPLAAISY
                     SAQLLAESEAMSEEDKRMVEIINNHCGRVNEIVENILQLSRRERSRPESLDLSVWAQH
                     FVDEYKDGNDIGADQLRAITQARQVEALADPQQLQQVVWNLVQNALRYGRQPGEPARV
                     AVVARMLTDKGPPLIEVVDRGPGIPPKVAAQIFEPFFTTHEYGTGLGLYLARTMCEAN
                     QATLEFVPVAGGGSCFRITLPAAPVLVRRAAT"
BASE COUNT          223 a          424 c          390 g          203 t
ORIGIN      
        1 cgctggtgct gggccggcag atgcgcgaga ccgaggcgct ggccgaacaa cgcggcatcg
       61 acctggccaa cctcgcccag atcaatgaac tcatcatccg gcgcatgaag accggcgtgc
      121 tggtggtgga cgacgccaac cgcgtgcatc gctggaacga atcggcctgg agcctgatcg
      181 gcaatcccaa atccgggcaa cgcgatctcg gcagcgttgc gccggaactg tcgcggcgcc
      241 tgtaccactg gcgcaccacc ggcaagatcg acgccactgc cgtggctctc gccgacggcg
      301 tgcccgaggt gatcccgcgc ttcacgcgcc tgtcctcgaa cgacgacgag aacgtgctga
      361 tcttcctcga cgacacctcg ctggtatcgc gccgtgccga ggaactcacc ctgtcctccc
      421 ttggccgcct gtccgcctcg atcgcgcacg aaatccgcaa cccgctcgct gcgatcagct
      481 actcggcgca gttgctcgcc gaatcggaag ccatgtccga ggaagacaag cgcatggtcg
      541 agatcatcaa caaccattgc ggccgcgtga acgagatcgt cgagaacatc ctgcaattgt
      601 cgcggcgcga acgctcgcgc ccggaaagcc tggatctttc cgtttgggcg cagcacttcg
      661 tcgacgagta caaggacggc aacgacatcg gcgcggacca gttgcgcgcg atcacgcagg
      721 cgcgccaggt cgaggcgctg gccgatccgc aacagttgca acaagtagtg tggaacctcg
      781 tgcagaacgc gttgcgctac ggccgccaac ccggcgagcc ggcgcgcgtg gcggtggtgg
      841 cgcgcatgct caccgacaag ggtccgccgc tgatcgaggt cgtcgaccgc ggccccggca
      901 tcccgccgaa agtcgccgcg cagattttcg aaccgttctt caccacgcac gaatacggca
      961 cgggcctcgg cctttatctc gcacgcacga tgtgcgaagc gaaccaggcc acgctcgaat
     1021 tcgtgcctgt cgccggcggc ggcagttgct tccgtatcac cctgccggcg gcaccggtat
     1081 tggtgcggcg ggcagcgacc tgaccggaat ccacgatgca gcgattcgcg aaacgcattg
     1141 gcgccttgcc gccgtgggtt ttgttcgccg gcgtgctggc gatcatggcc attgtctact
     1201 ggcccctgca cgccgcaggc ctggtctggg acgacaagtt
//
LOCUS       DASRVO010000168         3580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_264512, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000168 DASRVO010000000
VERSION     DASRVO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264512"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2682)
                     /gene="aceE"
                     /locus_tag="VFF05_01975"
     CDS             complement(<1..2682)
                     /gene="aceE"
                     /locus_tag="VFF05_01975"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZW50604.1"
                     /translation="MNNPLTAMLNQDPDPAETRDWLESIEAVIDAEGTERAHYLLEKL
                     VGETRRAGGHLPFSPTTEYINTIPPQLEAKSPGDANMEWRIRCILRWNAMAMVVRCNR
                     KPGSLGGHIASFASSATLYDVGFNHFWRAPSAEHPGDLIGYQGHSSPGIYARSFLEGR
                     IDAEQMDLFRLEVVAPERALASYPHPWLMPDYWQMPTVSMGLGPIQAIYQAQFWKYLE
                     NRGLMPKTDRKVWCFLGDGEVDEPESLGAISLAGREGLDNLIFVVNCNLQRLDGPVRG
                     NGKIIQELEGVFRGAGWNAIKVVWGSYWDPLLARDKDGILRKLMMETVDGEYQACKAF
                     GGKYTRENFFGKYPETLEMVRNLSDEDIWRLNRGGHDPHKVYAAYHAAVNTQGMPTVI
                     LAKTVKGYGMGAAGESQNPTHQQKKLDDDSVRHFRDRFQIPIADDKLHEVPYYHPGKD
                     SPEVQYMLERRKALGGFLPQRRRHTDEKFKAPELAEFAQITKGSGEREISNTMALVRG
                     MNLLLRDKAIGPRVVPIVADEARTFGMEGMFRQIGIYAPEGQKYRPQDADQLMYYREA
                     KDGQVLQEGISEAGGVSAWIAAGTSYSLSNKAMLPFFIYYSFFGFRRVGDLIWAAGDQ
                     RARGFLIGATAGGSFPGEGLQHSDCSSQLVAGTVPNCRAYDPTFSYEVAVLLHKGVQE
                     MFEQQLDVFYYITTTNENIVHPDMPEGCYEGIVKGMYLFQDAGKPKKNEPRVQLLAAG
                     GSVLEAVRAAALLDKEFGVKSDVWSCPSYSELSRDAYDCERWNRLHPEVKPRASHFVQ
                     CLEGHGPVVAVSEYVRAVADQVRAFVPEGRRFTALGADGFGRSDTREHLREFFEIDAR
                     WIAHAAVAALAADGKMSAKDVARAIKTWKLDPEKPNPL"
     gene            2839..>3580
                     /locus_tag="VFF05_01980"
     CDS             2839..>3580
                     /locus_tag="VFF05_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50605.1"
                     /translation="MTQPGPIDPATAQLIALETDIQAGRIDAAIATLKAFNHAYPTDV
                     RALLFDALIARAQIKTAHELMALHRATMQAPKWPRAFLELALTLSRENRHVEALTAAE
                     TAVALAPGERPPLETAIAVANGAGNAQAGLAFLKKAHALWPDDLVFDRQLGAMLTNLR
                     RHDEADRHWRELLTKHPGDSTAEVNYSMTLAALGRGDEAVALLERTHQREPDNATVAF
                     YLASARGETPASQPPELVQGIFDGYADYF"
BASE COUNT          607 a         1157 c         1146 g          670 t
ORIGIN      
        1 caacgggttg ggtttctccg gatcgagttt ccaggtcttg atcgcgcggg cgacgtcctt
       61 ggccgacatc ttgccgtcgg cggcgagcgc ggcgacggcg gcgtgggcga tccagcgcgc
      121 gtcgatctcg aagaattcgc gcaggtgctc gcgcgtgtcg gagcggccga agccatcggc
      181 gccgagcgcg gtgaagcggc ggccttccgg cacgaacgcg cgcacctggt cggcgacggc
      241 gcgcacgtat tcgctcaccg ccaccaccgg gccgtggcct tcgaggcatt gcacgaaatg
      301 cgaggcgcgc ggcttgactt ccggatgcaa gcggttccaa cgctcgcaat cgtaggcgtc
      361 gcgcgacagt tcgctgtagc tcgggcacga ccacacgtcg gacttgacgc cgaattcctt
      421 gtccagcaat gcggcggcgc gcaccgcttc gagcacggaa ccgcctgccg cgagcaactg
      481 cacgcgcggc tcgttcttct tcggcttgcc ggcgtcctgg aacaggtaca tgcccttgac
      541 gatgccctcg tagcaaccct cgggcatgtc cggatggacg atgttctcgt tcgtggtggt
      601 gatgtagtag aagacgtcca gctgctgctc gaacatttcc tgcacaccct tgtgcagcag
      661 cacggcgact tcatacgaga aagtcggatc gtaggcgcgg caattcggaa ccgtgcccgc
      721 gacgagttgc gacgaacagt ccgaatgctg caagccttcg ccggggaagc tgccgccggc
      781 cgtggcgccg atcaggaaac cgcgcgcgcg ctggtcgccg gccgcccaga tcaggtcgcc
      841 gacgcggcgg aagccgaaga acgagtagta gatgaagaac ggcagcatcg ccttgttcga
      901 cagcgaatag ctggtgccgg ccgctatcca ggccgacacg ccgccggcct cgctgatgcc
      961 ttcctgcagg acctggccgt ccttcgcctc gcggtagtac atgagctgat cggcgtcctg
     1021 cggccggtac ttctggcctt ccggcgcata gatgccgatc tggcggaaca tgccttccat
     1081 gccgaacgtg cgcgcctcgt cggcgacgat cggcactacg cgcgggccga tcgccttgtc
     1141 gcgcagcagc aggttcatgc cgcgcaccag cgccatggtg ttggaaatct cgcgctcgcc
     1201 cgagcccttg gtgatctgcg cgaattcggc cagctccggc gccttgaact tctcgtcggt
     1261 atggcggcgg cgctgtggca ggaagccacc gagcgccttg cgccgttcga gcatgtactg
     1321 tacttccggc gagtccttgc cggggtggta gtacggcact tcgtgcagct tgtcgtcggc
     1381 aatcggaatc tggaagcggt cgcggaaatg gcgcaccgaa tcgtcgtcga gcttcttctg
     1441 ctggtgcgtg ggattctgcg attcgccggc cgcgcccatg ccataaccct tgacggtctt
     1501 ggccaggatc accgtcggca tgccctgcgt gttcaccgcc gcgtggtagg ctgcatagac
     1561 cttgtgcgga tcgtggccgc cgcggttcag gcgccagata tcctcgtcgg aaagattgcg
     1621 caccatctcc agcgtttcgg gatacttgcc gaagaaattc tcgcgcgtgt acttgccgcc
     1681 gaacgccttg caggcctggt attcgccgtc gacggtttcc atcatcagtt tgcgcaggat
     1741 gccgtccttg tcgcgtgcaa gcagcggatc ccagtagctg ccccacacga ccttgatcgc
     1801 gttccagccg gcgccgcgga acacgccttc gagttcctgg atgatcttgc cgttgccgcg
     1861 caccggacca tcgaggcgct gcaggttgca gttgacgacg aagatcaggt tgtccaggcc
     1921 ttcgcgtccg gccagcgaga tggcgccgag cgattcgggc tcgtccactt cgccgtcgcc
     1981 gaggaaacac cagaccttgc ggtcggtctt gggcatcagg ccgcggtttt ccagatattt
     2041 ccagaactgt gcctgataga tcgcttggat cgggcccagg cccatcgaca ccgtcggcat
     2101 ttgccagtag tccggcatca gccacggatg cgggtacgac gccagcgcgc gctcgggtgc
     2161 gacaacttcg aggcggaaca agtccatctg ctcggcgtcg atgcggcctt cgaggaagga
     2221 acgcgcgtag atgccggggc tggagtggcc ttggtagccg atcaagtcgc ccggatgttc
     2281 ggcgcttggc gcgcgccaga aatggttgaa gccgacgtcg tacaacgtgg cagaggaggc
     2341 gaagctcgcg atgtgcccgc cgagcgagcc gggtttgcgg ttgcagcgca ccaccatcgc
     2401 catcgcgttc cagcgcagga tgcagcggat gcgccattcc atgttggcgt cgccggggct
     2461 tttcgcctcg agctgcggcg gaatcgtgtt gatgtactcg gtggtcggcg agaacggcag
     2521 gtgcccaccc gcgcggcgcg tctcgccgac gagcttttcg agcaggtaat gcgcacgctc
     2581 ggtgccttcg gcgtcgatca cggcctcgat ggattccagc caatcgcggg tttcggccgg
     2641 atcggggtcc tggttgagca tggcggtaag ggggttgttc atgagttcct cgcgtcggcg
     2701 cgtcgcgttt cgcgccagat gattgaagcg ttttcgtcag tttacccgag cgtgccaccg
     2761 gcggcacgcg gtggcgcgac cttgcgccac agcggcgacg gcatctaaag ttgcggttcg
     2821 ctatccggac ccctttccat gacacaaccc ggccccatcg atccggcgac cgcccaactc
     2881 atcgccctcg aaaccgatat ccaagctggt cggatcgatg cggcaattgc cacgctgaag
     2941 gcgttcaatc acgcgtatcc caccgatgta cgcgcgctcc tgttcgacgc gttgatcgcg
     3001 cgcgcgcaaa taaaaacagc gcacgaactc atggccttgc accgcgccac gatgcaggcg
     3061 ccaaaatggc cccgcgcctt cctggaattg gcgctgacac tttcgcgtga aaatcgtcat
     3121 gtcgaagcgc tgaccgcggc cgaaacggct gttgcgctcg cccccggcga acgaccgccg
     3181 ctggaaaccg ccatcgccgt ggccaacggt gcgggcaatg cgcaagcggg gctggctttc
     3241 ttgaaaaagg cgcatgcact gtggcctgac gacctagtct tcgaccgcca actcggcgcc
     3301 atgctgacca atctgcgccg ccacgatgag gccgacaggc attggcgcga gctgctgaca
     3361 aagcatccag gcgattctac ggccgaggtt aattacagca tgacccttgc cgccctcgga
     3421 cgcggcgatg aagcggtcgc cctgctcgaa cgcacgcatc aacgcgagcc cgacaacgcc
     3481 acggtcgcgt tctatctcgc ttccgcacgc ggggaaacac cggcctcgca gccaccagaa
     3541 ctcgtacagg gaattttcga cggttacgcc gattatttcg
//
LOCUS       DASRVO010000169         1745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_212598, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000169 DASRVO010000000
VERSION     DASRVO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212598"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VFF05_01985"
     CDS             <1..379
                     /locus_tag="VFF05_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZW50606.1"
                     /translation="PSFMQQFDVSLVDGAPPFANAQATHAAYQITLRDSGPLTEAHLL
                     VFADFVPPVALSWMAKPTPGSSLTWMFELLREDFTAYPLAGWRADAQLVAARDGYTSQ
                     STTLSAPDGTTIVLSRQSMVVFD"
     gene            453..650
                     /locus_tag="VFF05_01990"
     CDS             453..650
                     /locus_tag="VFF05_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZW50607.1"
                     /translation="MNPQQPLRRSRSDRKIAGVCGGLAKHFGLDPTYTRIGYVLLSVL
                     SAAFPGTLVYLILWWLVPEED"
     gene            685..>1745
                     /gene="dnaG"
                     /locus_tag="VFF05_01995"
     CDS             685..>1745
                     /gene="dnaG"
                     /locus_tag="VFF05_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZW50608.1"
                     /translation="MSGRIPEKFIDDLLARVDIVEVIEQRVPLKKAGRDWTARCPFHD
                     ERSPSFTVSPNKQFYHCFGCGAHGSAIKFLMEYDRMEFPDAVEELASRVGLKVPYEGG
                     AKRAPSEDSADLYTVLDACAKFYQRELGKSGKANAYFHARGLDAATIERFGLGYAPDG
                     WDGVKSALGTTPQRIALLEKGGMLARGERGTPYDRFRDRVMFPIQDRRGRVIAFGGRI
                     LEKTEDSGAKYLNSPETPLFHKGQQLFALWQVRQAHSKIPRLIVVEGYMDVIALFQHG
                     VTQAVATLGTATTRDHAELLFRNCADVYFCFDGDRAGRGAAWKAVESVLPRMRDGRQA
                     FFLFLPDGEDPDSLVRKEG"
BASE COUNT          310 a          584 c          540 g          311 t
ORIGIN      
        1 cccgagtttc atgcagcagt tcgatgtcag cctggtcgac ggcgcgccgc ccttcgcgaa
       61 cgcgcaagcg acacatgcgg cgtaccaaat caccttgcgc gattccggtc cactgaccga
      121 agcgcatctg ctcgtgttcg ccgatttcgt gccgccggtg gctctgtctt ggatggcaaa
      181 accgacgccg ggcagctcgc tgacctggat gttcgaactg ttgcgcgaag atttcaccgc
      241 gtatccgctc gcgggctggc gcgcggatgc gcaactcgtc gccgcgcgcg acgggtacac
      301 cagtcaatcg accacgttgt ccgcgcccga cggcacgaca atcgtcctca gccggcaaag
      361 catggtcgtt ttcgactgag taccccgatc tggttaccaa ccccgatcgg cccatcggta
      421 gaatggctgc atcgcctcgc ggagacaccg gaatgaatcc tcaacaaccc ctgcgccgct
      481 cacgcagcga ccgcaagatt gccggcgttt gcggcggctt ggccaagcat ttcggtctcg
      541 atcccaccta cacgcgcatc ggctacgtgc tgctgtcggt gctgtccgcc gcgtttcccg
      601 gcacgctggt ctacctgatc ctgtggtggc tggtgccgga ggaggattga gctccgcaag
      661 cgtcggcgcc atatcccaat cgccatgtcc ggccgcatcc ccgaaaaatt catcgacgac
      721 ctgctcgccc gcgtcgacat cgtcgaggtg atcgagcagc gcgtaccgct gaaaaaggcc
      781 gggcgcgact ggaccgcgcg ctgtcctttc catgacgaac gctcgccctc gttcacggtc
      841 agcccgaaca agcagttcta ccattgcttc ggctgcggcg cgcatggcag cgcgatcaag
      901 ttcctgatgg aatacgaccg catggaattt cccgacgcgg tcgaggagct cgcctcgcgc
      961 gtcggcctga aggtgcctta cgaaggcggc gccaagcgcg caccgagtga ggattcggcg
     1021 gacttgtaca ccgtgctcga tgcctgcgcg aagttctacc agcgcgaact cggcaagagc
     1081 ggcaaagcga acgcctactt ccacgcgcgc ggcctggacg ccgcgaccat cgaacgcttc
     1141 ggcttgggct acgcaccgga cggctgggat ggtgtcaaat ccgcgctcgg cacaacgccg
     1201 cagcgcatcg cgctgctgga aaaaggcggc atgctcgcgc gcggcgaacg cggcacgccg
     1261 tacgaccgct ttcgcgaccg cgtgatgttc ccgatccagg atcggcgagg gcgcgtgatc
     1321 gctttcggtg gccgcatcct cgagaaaacc gaagactcgg gcgcgaaata cctcaactcg
     1381 cccgagactc cattgttcca caagggccag caactgttcg cgctgtggca ggtacgccag
     1441 gcgcatagca agattccgcg cctgatcgtg gtcgaaggct acatggacgt gatcgcgctg
     1501 ttccagcacg gcgtgaccca ggccgtggcc acgctcggca cggcgaccac tcgcgatcac
     1561 gctgagctgt tgtttcggaa ttgtgcggat gtctacttct gcttcgacgg cgaccgtgcc
     1621 ggacgcggcg ccgcatggaa ggcggtcgaa tccgtgctgc cgcgcatgcg cgacgggcgc
     1681 caggcgttct tcctgttcct gcccgacggc gaagacccgg attcactggt gcgcaaggaa
     1741 ggcca
//
LOCUS       DASRVO010000170         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_82983, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000170 DASRVO010000000
VERSION     DASRVO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82983"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /gene="sufB"
                     /locus_tag="VFF05_02000"
     CDS             <1..1280
                     /gene="sufB"
                     /locus_tag="VFF05_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016902344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HZW50609.1"
                     /translation="RLKAYRHWLTMTPPQWAKLQIAPIDFQAISYFSRPKNQPKSLAE
                     VDPKLLETYDKLGVPLHERAKLAGVAVDVVFDSVSVHTTVHKQLSDAGVIFCSMSTAI
                     KEHPELVRKYLGSVVPQGDNYFAALNSAVFSDGSFVFIPKGVRCPMELSTYFRINAGH
                     TGQFERTLIIAEEGASVSYLEGCTAPMRDENQLHAAVVELVALDDAKIKYSTVQNWYP
                     GDENGVGGIYNFVTKRGDCRGARSRIVWTQVETGSAITWKYPSCILRGDDSAGEFHSV
                     ALTHHRQQADTGTKMIHIGKNTKSKIVSKGISAGRGQNTYRGLVKIEKGASGARNHTQ
                     CDSLLIGKQCGAHTFPYIEVKNPGAIVEHEATTSKISDDQLFYCRSRGIGEEDAVSMI
                     VDGFCKQVFKELPMEFAVEAKKLLEVSLEGAVG"
     gene            1277..1570
                     /locus_tag="VFF05_02005"
     CDS             1277..1570
                     /locus_tag="VFF05_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50610.1"
                     /translation="MNVVAARSSGLALATLIATTVALAGTLVAAALHAQIWLSTWLEV
                     LMAVSFVASIVGAIGGLLMASRRRNVMWILAVSVSAAALMVLLAVVASSFGPD"
BASE COUNT          320 a          499 c          515 g          306 t
ORIGIN      
        1 ggcggctcaa ggcctatcgg cactggctga cgatgacgcc gccgcagtgg gcgaagctgc
       61 agatcgcgcc catcgatttc caggcgatca gttatttttc gcggccgaag aaccagccca
      121 agtcgttggc cgaagtcgat ccgaaactgc tggaaaccta cgacaagctt ggcgtaccgc
      181 tgcacgagcg cgccaagctc gccggcgtcg cggtcgatgt ggtgttcgat tcggtgtcgg
      241 tccacaccac ggtgcacaag cagctgtccg acgcaggcgt catcttttgc tcgatgtcca
      301 cggcgatcaa ggaacatccc gaactggtgc gcaaatacct gggctcggtg gtgccgcagg
      361 gcgacaatta tttcgcggcg ctgaactccg cggtgttttc cgacggcagc ttcgtgttca
      421 tccccaaggg cgtgcgctgc ccgatggaat tgtccacgta cttccgcatc aatgccggcc
      481 acaccggcca gttcgagcgc accctgatca tcgccgagga aggcgcgagc gtgtcgtacc
      541 tggaaggctg caccgcgccg atgcgcgacg agaaccagtt gcacgcggcg gtggtcgaac
      601 tcgtcgccct ggacgacgcc aagatcaagt attcgaccgt gcagaactgg tatcccggcg
      661 acgagaatgg cgtcggcggc atctacaact tcgtcaccaa gcgcggcgac tgccgcggcg
      721 cgcgttcgcg catcgtgtgg acgcaggtgg aaaccggttc cgcgatcacc tggaaatacc
      781 cgagttgcat cctgcgtggc gacgattcgg ccggcgagtt ccattcggtc gcgctgaccc
      841 atcatcgcca gcaggccgac accggcacga agatgatcca cattggtaaa aatacaaaaa
      901 gcaagattgt ttcaaaaggt atttccgcag gacgcggcca gaacacctac cgcggcctgg
      961 tcaagatcga gaagggcgcc agcggcgcgc gcaaccacac ccagtgcgat tcgctgttga
     1021 tcggcaagca gtgcggcgcg cacacgttcc cgtacatcga agtgaagaat cccggcgcca
     1081 tcgtcgagca cgaggcgacc acgtcgaaga tttccgacga ccagcttttc tattgccgct
     1141 cgcgcggcat cggcgaggaa gacgcggtgt cgatgatcgt cgacggtttc tgcaagcagg
     1201 tgttcaagga attgccgatg gaattcgccg tcgaggcgaa gaagttgctc gaagtgagtc
     1261 tggaaggagc ggtgggatga acgtcgtcgc cgcacgcagt tccggactgg ctctcgccac
     1321 actgatcgcc acaacggttg ccttggccgg cacgctggtc gctgccgccc tgcacgcgca
     1381 gatctggctg tcgacatggc tcgaggtgtt gatggcggtg agctttgtcg cgtccatcgt
     1441 cggcgccatc ggcgggttgc tgatggcgag ccggcgccgc aacgtgatgt ggatactcgc
     1501 tgtctccgta tcggcggcgg ccctcatggt gctgctcgcc gtagtggcgt cctcgttcgg
     1561 accggattga acccatgcac atgctgaaaa tcgacaatct ccacgttcgc gtcgcgggca
     1621 aggaaatcct caagggcctt
//
LOCUS       DASRVO010000171         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_82996, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000171 DASRVO010000000
VERSION     DASRVO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82996"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VFF05_02010"
     CDS             complement(<1..970)
                     /locus_tag="VFF05_02010"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HZW50611.1"
                     /translation="MSYFIAGPIARIGQRLPGGTWKKIVTRNAARETVAADGGRLAFD
                     NYETQCEDLIPLVVDLDGTLLKSDSLIEGFVSLLSTRPVAALWTLAALRRGKAAFKAA
                     VAREAKIDIVGLPWNEAVLELVRAARAAGRKVYIASASDRSLVEAMARHLGLFDGTFC
                     SDGAVNLSGRAKSRALCAAFGAGGFDYAGNSQIDCAVWECARNVIVVDAPRTLVTKVR
                     RRWPGARVIARQGGGLKTYLRAIRVHQWLKNGLIFVPMLAAKQFGAHELANSALAFLS
                     FSLCASAVYVMNDLIDLSRDRAHATKRTRPFAAGTLPVTHGLALAPL"
BASE COUNT          215 a          340 c          332 g          175 t
ORIGIN      
        1 gcaacggcgc gagcgcaaga ccgtgcgtca ccggcaacgt gccggccgcg aaaggccgcg
       61 ttcgcttggt ggcatgtgcc cgatcgcggc tcaaatcgat cagatcgttc atcacataga
      121 cggcggacgc gcacaggctg aagctcaaaa atgccagcgc ggagttcgcg agctcatgcg
      181 cgccgaactg cttggcagcc agcatcggca cgaagatcag cccgttcttt agccattgat
      241 ggacgcggat ggcgcggaga taggtcttca gcccgccgcc ttgccgcgcg atgacgcgcg
      301 cgccgggcca gcggcgccga accttcgtca ccagcgtccg tggcgcgtcg acgacgatca
      361 cgttgcgcgc gcattcccaa accgcgcaat cgatctggga attgccggca tagtcgaagc
      421 cgccggcgcc gaaagcggcg cagagcgcgc ggctcttggc acggccggac agattgaccg
      481 cgccgtccga gcagaaggtg ccgtcgaaca gtccgagatg gcgggccatg gcctcgacca
      541 ggctgcggtc tgaggcggat gcgatataga ccttccgtcc agcagcgcgc gcggcgcgca
      601 caagctcgag gaccgcctcg ttccacggca agccgacgat gtcgatcttc gcctcgcgcg
      661 cgaccgccgc cttgaacgcc gccttgccgc gccgaagcgc cgccagcgtc cacaaggccg
      721 cgacgggtcg ggtgctgagg agcgacacaa agccttcaat aaggctgtcg gatttgagca
      781 acgtcccgtc gaggtctaca accagaggta ttaaatcttc acattgagtc tcgtagttat
      841 caaaagcgag gcgaccacca tccgccgcaa cggtttcacg cgctgcgttt cgagtgacga
      901 tctttttcca agtacctcca ggcaggcgtt gcccgatgcg tgcgatcggg cccgcgatga
      961 aatacgacat gctttggcca taggcggcag attactgaaa cgacaaacgc cgcataggtt
     1021 gcgaggttcc actcggcctc taaaagctca gcaagttgtg at
//
LOCUS       DASRVO010000172         1355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_212622, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000172 DASRVO010000000
VERSION     DASRVO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212622"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFF05_02015"
     CDS             complement(<1..1334)
                     /locus_tag="VFF05_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50612.1"
                     /translation="MRSWTAPNPIPTNNRVAPYETTVWVLDATLTEYKLENDSDYHLV
                     IKDASGNTMIAEIPDPACVGSGSPFASDIQNARAQFDAKYTATTSFQTVSIPVQLIGM
                     GMFDFLHGQTGVAPNGIELHPVLGIVFNPSGGGSPDFALSASPQSVSVEQGAGASSTI
                     AVTPSNGFASAVSFSATGLPSGVTASFSPASSAASTTLTLNAGSTAATESAAVTIKCT
                     SGSLSHATTVNLTVVASGGGGSSTAVYNSTLKAPECAAAGASCDSGTLLVGRGTMSGG
                     VEPNQPNTINASCADGNSGTFHSDESNDRIVISSTDGGPLTAGKTAQVTATMWAYSSA
                     NAIDFYSAANASNPSWTFLSSISASAAGTQSLSTTFTLPAGGLQAVRAHLRYNGSASS
                     CSTGSYDESDDLIFTVSANAPAPDFGIAVSPASVNVTQGGNGASSVTISPQNG"
BASE COUNT          220 a          423 c          464 g          248 t
ORIGIN      
        1 ccgttctgcg gcgagatcgt caccgagctg gcgccgttgc cgccctgcgt cacgttcacc
       61 gacgccggcg agacggcgat gccgaagtcc ggtgccggcg cgttcgcgga caccgtgaag
      121 atcagatcgt cggattcgtc gtagctgccg gtgctgcacg acgacgcgct gccgttgtag
      181 cgcaagtgcg cgcgcaccgc ctgcaagccg ccggccggca gggtgaacgt cgtggacaac
      241 gactgcgtgc ctgccgcgga agcgctgatc gaactgagga acgtccagct cgggttgctg
      301 gcattcgcgg ccgagtagaa gtcaatcgcg ttcgccgaac tgtatgccca catcgtggcg
      361 gtgacctgcg cggtcttgcc cgcggtcagc ggtccgccgt cggtggaact gatcacgatg
      421 cgatcgttgg actcgtccga atggaaggtg ccggaattgc cgtcggcgca ggacgcattg
      481 atcgtgttcg gctggttcgg ctcgacgccg ccggacatcg tgccgcgtcc gaccaggagg
      541 gtgccggaat cgcaggacgc gccggccgcg gcgcactccg gcgccttcaa tgtggaattg
      601 tatacagccg tggagctgcc accgccgccg ctggcgacta cggtgaggtt caccgtcgtg
      661 gcatgcgaca ggctgccgga ggtgcacttg atcgtgacag cggccgattc cgtcgccgcc
      721 gtgctgccgg cgttcagggt caacgtggtc gatgccgcgg aactggccgg actgaacgac
      781 gcggtcaccc cgctcggcaa tcccgtggca ctgaacgaca cggcgctggc gaagccgttc
      841 gacggcgtca ccgctatcgt gctgctggcg ccggcgccct gctccacgct cacgctctgc
      901 ggcgaagcgg acagggcgaa atcgggcgat ccgccaccgc ttggattgaa cacgatgccc
      961 agcaccgggt gcagttcgat cccgttcggc gccacgccgg tctggccatg caggaagtcg
     1021 aacatgccca tgccgatcaa ctggaccggg atgctgacag tctgaaaact ggtggttgcg
     1081 gtgtacttcg cgtcgaactg cgcgcgcgcg ttttgaatgt cgctggcgaa tggactgccc
     1141 gaaccgacgc aggccggatc cggaatctcg gcaatcatgg tgttgccgga cgcgtccttg
     1201 atgaccaggt ggtaatccga atcgttctcg agcttatact cggtgagggt cgcatcgagc
     1261 acccacacgg tcgtttcgta gggcgcgacg cgattattcg ttggaatcgg attgggtgcg
     1321 gtccagctac gcatcgtgac gatgtcgttc ggctg
//
LOCUS       DASRVO010000173         1415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_4893, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000173 DASRVO010000000
VERSION     DASRVO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4893"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFF05_02020"
     CDS             <1..943
                     /locus_tag="VFF05_02020"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HZW50613.1"
                     /translation="PKLHVDRARVAVAGMSAGAYMAEQAQLAYPEIFHGAALIAGGPY
                     DCAQDSVATAVTTCMKGSPAPDPIALAKLAQERAAKGEIGPLAKLARGKVYILHGKQD
                     PVIAESVAHATVDFYAALKAAVPALSSLTVTWDGSHDFSHTFPTEAAGIDCDKVGTPF
                     LGKCGFDGAGAVFHALYGAPLHKVAEAKGKLQRFDQKTLLSDGKDASLADAGVAYVPP
                     ACAKGRRCGVLVVLHGCLQNVDSVGESFVKEAGFNRWADAYDVAVLYPQTRSGVVANP
                     KACWDWFGYTGADYATRNGLQLRWLVAAVTALGRPSH"
     gene            1045..>1415
                     /locus_tag="VFF05_02025"
     CDS             1045..>1415
                     /locus_tag="VFF05_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZW50614.1"
                     /translation="MAMWKLEATTCACAALVLAGCAVGPDFHKPGAPDAKAYDAGALP
                     AQTASAPVTGGDAQRFEGGADIPGDWWTLFQSPALDALIERALKNNPDLAAARAALTV
                     AQENTLAQRGAYFPSVDADFSS"
BASE COUNT          232 a          466 c          481 g          236 t
ORIGIN      
        1 gccgaagctg cacgtcgatc gcgcccgcgt cgcggtggcg ggcatgtccg caggcgccta
       61 catggccgag caggcgcaac tggcctatcc ggaaattttc catggcgcgg cactgatcgc
      121 cggcggaccc tacgactgcg cgcaggacag cgtcgccacc gccgtcacca cctgcatgaa
      181 aggctcgccg gcgccggatc ccatcgcact ggccaagctc gcgcaggagc gcgccgcaaa
      241 aggcgagatc ggtccgctcg ccaagctggc gcgcggcaaa gtatacattt tgcacggcaa
      301 gcaggatccc gtgatcgccg aatccgtggc ccacgccacc gtcgatttct acgctgcgct
      361 caaggccgcc gtgccggcgt tgtcgtcgtt gacggtcacc tgggacggca gccacgattt
      421 cagccacacg ttcccgaccg aagcggcagg gatcgactgc gacaaggtcg gcacgccgtt
      481 cctcggcaaa tgcgggttcg acggcgccgg cgccgtgttc catgcgttgt acggtgcgcc
      541 gctgcacaag gtggcagaag ccaaaggcaa gctgcagcgt ttcgaccaga aaacgttgct
      601 gtcggatggc aaggatgcgt cactcgcgga cgcgggcgtc gcctacgttc cgcccgcctg
      661 cgcgaagggc cgtcgctgcg gcgtactggt cgtgctgcac ggctgcctgc agaacgtcga
      721 cagcgtcggc gagtctttcg tgaaggaggc cggcttcaac cgctgggccg atgcctacga
      781 cgtggccgtg ctgtatccgc agacgcgctc gggcgttgtc gcgaacccca aggcctgctg
      841 ggactggttc ggctataccg gcgcggacta cgcgacgcgc aatggcttgc aactgcgctg
      901 gctggtggcg gcggtgacgg cgctcggacg gccttcgcat tgaagccgac tctcgcgcgg
      961 cgcagcgcct tcggcattcc ttaacgattt tctgatacat tatatcgttg caaggccggc
     1021 tccgccggtt atggcggatc cggcatggcg atgtggaagc tcgaagcgac aacctgcgcg
     1081 tgcgcggcac tggtgctcgc cggctgcgcg gtcgggccgg atttccacaa gcccggcgcg
     1141 ccggatgcga aggcttacga cgcgggcgcg ctgccggcgc aaacggccag cgcgccggtg
     1201 accggtggcg atgcgcaacg cttcgaaggc ggcgcggata ttcccggcga ctggtggacg
     1261 ttgttccagt cgccagcgct ggacgcgctg atcgaacgcg cgctgaaaaa caatcccgat
     1321 ctcgccgccg cacgtgcggc gttgacggtg gcgcaggaaa acacgctggc tcagcgcggc
     1381 gcctatttcc catcggtcga cgcggatttt tcgtc
//
LOCUS       DASRVO010000174         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_187230, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000174 DASRVO010000000
VERSION     DASRVO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187230"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFF05_02030"
     CDS             complement(<1..656)
                     /locus_tag="VFF05_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZW50615.1"
                     /translation="MRILLVEDDQRIASDVSRALEAANYVVETVRDGEEAWFRGDTED
                     YGAVILDLGLPGMDGLAVLKRWRANGRHMPVLILTARASWAERVDGIDAGADDYLPKP
                     FRIEELLARLRSIVRRSAGHASSVVSAGDITLDERQMRVSVRGVPVTMSPLEYRLVAY
                     LLRHRGRVVSQHELDENVYGHGEDHDSNALEVLIGRVRKKLGPDLIETRRGFGYIVPE
                     A"
     gene            complement(656..964)
                     /locus_tag="VFF05_02035"
     CDS             complement(656..964)
                     /locus_tag="VFF05_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZW50616.1"
                     /translation="MLAMVLAAAALVAAAPRVAGARDQDEQRRDEVRRAVESGEFHSL
                     ADILNTLRGKLPGEVAGVEIEHEEGHWFYEFRVVDSQGRLFEVYVDPRSGEIERIKEK
                     "
BASE COUNT          199 a          380 c          330 g          198 t
ORIGIN      
        1 gcctccggca cgatgtagcc gaagccgcgg cgggtctcga tcagatcggg tcccagtttc
       61 ttgcgcacgc ggccgatcaa tacttccagc gcattggaat cgtgatcttc gccatggccg
      121 tacacgttct cgtcgagttc gtgctgcgac accacacggc cgcggtggcg cagcaggtaa
      181 gcgacgaggc gatattcaag cggcgacatc gtgacaggca cgccgcgcac gctcactctc
      241 atttgtcgtt catcgagcgt gatgtcaccg gctgacacca ccgacgatgc atggccggcg
      301 gagcggcgga cgatcgagcg cagccgcgcc agcaattctt cgatccggaa cggcttcggc
      361 agatagtcgt cggcgccggc gtcgattccg tcgacgcgtt cggcccagct ggcgcgcgcc
      421 gtcaggatca gcacaggcat gtgacgcccg ttcgcgcgcc agcgcttgag caccgcaagt
      481 ccgtccatgc ccggcagccc gagatcgagg atgaccgcgc cataatcctc ggtgtcaccg
      541 cggaaccatg cttcttcgcc gtcgcgcact gtctcgacca cgtaattcgc ggcctcaagc
      601 gcacgcgaaa cgtccgatgc gatccgctgg tcgtcctcga caagaagtat acgcatcatt
      661 tctccttgat acgctcgatc tcgccgctgc gcgggtcgac atagacttcg aacaaccggc
      721 cctggctgtc gacgacacgg aattcgtaaa accagtggcc ttcctcgtgt tcgatctcga
      781 cgccagcgac ctcgccgggc agcttgccgc gcagggtgtt gagaatgtcc gccagggaat
      841 ggaactcgcc gctctcgact gcgcggcgga cctcgtcgcg gcgctgctcg tcctgatcgc
      901 gcgcgcccgc cacgcgcggc gcggccgcaa ccagcgcggc tgccgcgagc accattgcca
      961 acattgcagt tcgagccgag cgcatggacc gttacaccaa ttttaccggc gccagcatgc
     1021 ccgaaacgac ctgacacatt gctgacatgg atcaacaggc cttcgtcatc gctcggcgcg
     1081 cattgtcgca ggcgtcctgg ccgaata
//
LOCUS       DASRVO010000175         3341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_134779, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000175 DASRVO010000000
VERSION     DASRVO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134779"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..707)
                     /locus_tag="VFF05_02040"
     CDS             complement(12..707)
                     /locus_tag="VFF05_02040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZW50617.1"
                     /translation="MAESELDNPFWSSLQTRHRAIALRAGDAARYPAEFAPFVGVAHA
                     DVDAGDALARLIGPEENLLLLGVAPRVSKGWVLEAFQPLAQMIRTAPIAVIDGPPIID
                     LSEGHRADVLALTALVYPHYFRERTMELGRYFGIYMPDQRGDVRLAAMIGERLGTDTH
                     TEMSAICTHPDFNGRGYARRLTAHLTNDTLAHGRTPFLHVSHENVRALQLYERMGYTL
                     RRDIGFWALHRAA"
     gene            752..1819
                     /locus_tag="VFF05_02045"
     CDS             752..1819
                     /locus_tag="VFF05_02045"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014505959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZW50618.1"
                     /translation="MKTPAYAAQAADKPLAFHAIDRRAPGPRDVAIDISHCGVCHSDL
                     HTARSEWGGTLYPCVPGHEIVGTVTAIGKDVKKFKVGETVGVGCLVGSCGHCASCAEG
                     LENYCENGFVGTYNGPAPAAEGGHTFGGYSTHIVVDEGFVLHIRHKGELAAVAPLLCA
                     GITTYSPLMHWKAGPGKTIGIVGLGGLGHMGVKLAHAMGARVALFTTSPGKEADAKRL
                     GADDVVISKDAKQMAKQANRFDLIVNTVAAPHNLDVFLNLLKRDGTMCLVGAPATPHP
                     STQVFNLIFKRRAIAGSLIGGIPETQEMLDFCAERGIVSEIETIAMRDINTAYERMLK
                     SDVKYRFVIDMRTLRNEAKAA"
     gene            complement(1842..2129)
                     /locus_tag="VFF05_02050"
     CDS             complement(1842..2129)
                     /locus_tag="VFF05_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019281521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZW50619.1"
                     /translation="MTYSLAFVESALKEWRKLASPIREQFKKKLAERLEHPRVPAARL
                     HNLPDCYKIKLRSAGYRLVYQVDDKIVLVTVIAVGKRDKQRVYSRAAKRMS"
     gene            complement(2119..2364)
                     /locus_tag="VFF05_02055"
     CDS             complement(2119..2364)
                     /locus_tag="VFF05_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HZW50620.1"
                     /translation="METILADASVSITELKRNPSAVIEAADDEAIAVLVHNKPSAYLV
                     PAETYRRLMDRLEDLELAELVRARAKERRVKVKFDDL"
     gene            complement(2414..3121)
                     /locus_tag="VFF05_02060"
     CDS             complement(2414..3121)
                     /locus_tag="VFF05_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50621.1"
                     /translation="MRNLDFTSWHTLLSTLLGLAFITLIGVGIRLLAQQTIQRRRERE
                     NRQINERLRTLIAAYKTLGGSFTGNLMVDPTHLRDLRHRGEMAGTAAPSDVPLADAGS
                     GSDRARRIRDAVEAALSDIVLLGTADQVRMAASAAAELVARRPVHTHDLVVSLRDFIR
                     TALDLEPIPADATIPRQCPARTPASRGGIGEGGKADAKGGRGGGGGGMGGMGAGMGMG
                     AGMGAGLGGHVDDEEHR"
BASE COUNT          610 a         1126 c         1038 g          567 t
ORIGIN      
        1 cttaacaccg gctacgcggc tcgatgcaag gcccagaagc cgatgtcccg acgcaacgtg
       61 tagcccatgc gttcgtaaag ctgcaaggcg cgcacgttct catggctgac gtgcaggaac
      121 ggcgtgcgtc cgtgcgccag cgtgtcgttg gtgaggtgcg cagtcagtcg gcgcgcgtag
      181 ccgcggccgt tgaaatccgg atgcgtgcag atcgcgctca tctccgtatg cgtgtcggtg
      241 ccaaggcgct cgccgatcat ggccgccagg cggacatccc cgcgctggtc cggcatgtag
      301 atgccgaagt agcggcccag ttccatcgtg cgttcgcgga aataatgcgg atacaccagc
      361 gccgtcagcg ccagcacgtc ggcacggtgc ccctcggaca aatcaatgat cggcggacca
      421 tcgatcaccg cgatgggtgc cgtacggatc atctgcgcga gcggctggaa cgcttccaac
      481 acccagcctt tggaaacgcg cggtgcgacg ccgagcagga gcaggttttc ttccggccca
      541 ataagtcgcg caagcgcatc gcctgcgtcg acgtccgcat gcgcaacgcc cacgaacggc
      601 gcgaattccg ccggatatcg cgcggcatcg ccggcacgca aggcgatcgc gcgatgccgc
      661 gtttgcaggg acgaccagaa ggggttgtcg agttcggatt cggccacggt ggtatcttag
      721 cttcaaccca tgcgctgatt tcggagaagt catgaaaacc cccgcctatg ccgcacaggc
      781 cgccgacaag ccgctcgcct tccacgccat cgatcgtcgc gcgccgggac cgcgcgacgt
      841 cgccatcgac atttcgcatt gcggcgtctg ccattccgac ctgcacaccg cacgcagcga
      901 atggggcggc acgctgtatc cgtgcgtgcc cggccacgag atcgtcggca ccgtcaccgc
      961 aatcggcaag gacgtgaaga aattcaaggt cggcgaaacc gtcggcgtcg gctgcctcgt
     1021 cggcagttgc gggcattgcg catcctgcgc ggaaggtctc gagaattatt gcgagaacgg
     1081 cttcgtcggc acctacaacg gtccggctcc ggcggcggaa ggcggccaca ccttcggcgg
     1141 ctactcgaca catatcgtcg tcgacgaagg tttcgtcctg cacatccgcc acaagggtga
     1201 actcgcggcc gttgcaccgc tgctgtgcgc aggcatcacc acctattcgc cgctcatgca
     1261 ctggaaggcc ggaccgggca agaccatcgg catcgtcggc ctcggcggac tcggccacat
     1321 gggcgtgaag ctcgcgcacg caatgggcgc gcgcgtggcg ttgttcacga cctcgccggg
     1381 caaggaagcc gacgccaagc gtctgggcgc cgacgacgtg gtgatctcca aagacgccaa
     1441 gcagatggcg aagcaggcca accgcttcga cctgatcgtc aatactgttg ccgcgccgca
     1501 caacctcgac gtcttcctca acctgctcaa gcgcgacggc acgatgtgcc tggtcggtgc
     1561 gccggcgacg ccgcacccct cgacgcaggt gttcaacctg atcttcaagc gccgcgccat
     1621 tgccggatcg ctgatcggcg gcatcccgga aacccaggag atgctcgact tctgcgccga
     1681 acgcggcatc gtctccgaga tcgagacgat cgccatgcgc gacatcaaca ccgcctacga
     1741 acgcatgctc aagagcgacg tgaagtatcg tttcgtgatc gacatgcgga cgttgcgcaa
     1801 cgaggcgaag gcggcataag cactcagcca atcaggcgcc atcacgacat gcgcttggcg
     1861 gcacgcgaat aaacgcgctg cttgtcacgc ttgcccacgg cgatcaccgt gacgagcacg
     1921 atcttgtcgt caacctggta aaccaaccgg taaccggcgc tgcgcagctt gatcttgtag
     1981 caatctggca aattgtgcag gcgcgcggcc ggcacacggg gatgttcaag gcgctcggcg
     2041 agcttctttt tgaattgctc gcgaatcggc gacgccagct tgcgccattc cttgagcgcg
     2101 gattcgacga acgcgagact ataggtcatc gaacttgacc ttgacgcgcc gctccttggc
     2161 acgtgcacgg accagttcgg caagctcgag atcctccaac cgatccatca ggcgccggta
     2221 cgtttcggca ggcaccagat aggcgctggg cttgttgtgc accagcaccg ctatggcttc
     2281 gtcgtcggcc gcttcaatca ccgcactcgg gttacgtttg agttcagtga tgctgacgct
     2341 ggcatcggca aggatcgttt ccatattcgg ctccaaattg ggagctaaat atagcacatt
     2401 tcaatacctc gccctagcga tgctcctcat cgtcgacatg gccgccgagc ccggcgccca
     2461 tgcctgcgcc cattcccatg ccggcgccca tgccacccat cccgccgccg cctccacccc
     2521 ggccgccttt cgcgtcggcc ttgccgccct cgcctattcc gccgcgcgat gccggcgtgc
     2581 gcgccgggca ctggcgcggg atcgtcgcat ccgccggtat cggctccaga tcgagcgccg
     2641 tgcggatgaa atcgcgcagc gaaaccacca ggtcgtgcgt gtgcaccgga cgccgggcga
     2701 cgagttccgc cgcggcgctt gccgccatcc gcacctggtc cgcggtgccg agcaggacga
     2761 tgtccgacag cgcggcctcg accgcgtcgc ggatgcgccg ggcgcgatcg ctgccggatc
     2821 ccgcgtcggc cagcggcacg tccgaaggcg ccgccgtacc tgccatctcg ccgcgatgcc
     2881 gcaagtcgcg cagatgggtc gggtccacca tcaggttgcc ggtgaatgat ccgcccagcg
     2941 tcttgtaggc cgcgatcagc gtgcgcaggc gttcgttgat ctgccggttc tcgcgctcgc
     3001 gccggcgctg gatcgtctgc tgcgccagca gccggatgcc cacgccgatc agggtgatga
     3061 aagccaggcc cagcagcgtc gacagcaacg tgtgccagga cgtgaagtcg agattgcgca
     3121 tgagcggact ccggcagcgt ggactgcgtg atcactttac gcgatcgcgc ggcaatgttc
     3181 gtgcgctgcc gccgggtcag gcccgataaa agtcctgcac gcggtaacgc cgcgcgaaca
     3241 gggcgaacac cagcgcgatc gccagcgcga acacggcaaa gaagaacatc aggaatgcgt
     3301 tctcgctcaa ccctgtagcg gtgatgcgcg ccgtgaccgc c
//
LOCUS       DASRVO010000176         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_291148, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000176 DASRVO010000000
VERSION     DASRVO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291148"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /gene="purT"
                     /locus_tag="VFF05_02065"
     CDS             <1..163
                     /gene="purT"
                     /locus_tag="VFF05_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015472403.1"
                     /note="non-folate utilizing enzyme, catalyzes the
                     production of beta-formyl glycinamide ribonucleotide from
                     formate, ATP, and beta-GAR and a side reaction producing
                     acetyl phosphate and ADP from acetate and ATP; involved in
                     de novo purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase 2"
                     /protein_id="HZW50622.1"
                     /translation="EALAEPDTMLRLFGKPEVDGHRRMAVTLARDASTDAACAKANRA
                     AAYITVDLG"
     gene            complement(144..623)
                     /gene="trmL"
                     /locus_tag="VFF05_02070"
     CDS             complement(144..623)
                     /gene="trmL"
                     /locus_tag="VFF05_02070"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HZW50623.1"
                     /translation="MLHVVLYEPEIPPNTGNVIRLCANSGARLHLIRPLGFDLDHAKL
                     RRAGLDYHEFADVAVHDDLDAFITDARPARLFALSTRGRHLYTNVRFGADDAVLFGPE
                     TRGLPQAVIDGLPPEQRLRLPMRPDNRSLNLSNAVAVVVYEAWRQMDFAGAASPDRP"
     gene            complement(628..>1174)
                     /locus_tag="VFF05_02075"
     CDS             complement(628..>1174)
                     /locus_tag="VFF05_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50624.1"
                     /translation="AQAPDFSMLDARWGYREGVRGNGKTREIFVQRAPDQAQKSAPLD
                     LPPDGVIDAGFDAYVRRHWDALAHGDALRFKFLVPSKRTFYAFDLSRVDAASDATRLT
                     LRLALGAWYAFLAPHIDVTYDRSTRRLLRFAGMSNIRDEKLKNLDVRIEFPQAPVAAT
                     TEQVAAARNAPLATSCAVVGK"
BASE COUNT          212 a          380 c          401 g          181 t
ORIGIN      
        1 cgaggcgttg gccgaacccg acaccatgct gcgcctgttc ggcaagcccg aggtcgatgg
       61 ccaccggcga atggcggtga cgctggcgcg cgacgcgagc accgacgccg cgtgcgcgaa
      121 ggcgaatcgc gcggcggcgt acatcacggt cgatctgggc tagccgcgcc ggcaaaatcc
      181 atctgccgcc acgcctcgta cacgacgacg gcgacggcgt tggagagatt caagctccgg
      241 ttgtccggcc gcatcggcag gcgcagacgt tgctccggcg gcaagccgtc aatcacggcc
      301 tgcggcaggc cgcgcgtctc cgggccgaac agcaccgcgt cgtccgcgcc gaatcgtaca
      361 ttcgtataca aatgccggcc acgcgtggac agcgcgaaca gccgtgccgg gcgcgcatcc
      421 gtgatgaagg cgtcgagatc gtcgtgcacg gcgacgtcgg cgaattcgtg gtagtcgagc
      481 ccggcgcggc gcagcttggc gtggtcgaga tcaaaaccaa gcggacggat caggtgcaat
      541 cgcgcgccgg aattcgcgca caggcggatc acgttgccgg tgttgggcgg gatttccggc
      601 tcatacaaga cgacgtgcag cacgcggtca tttcccgact acagcgcacg atgtggcgag
      661 cggtgcgttg cgcgccgcgg ctacctgctc agtcgtcgcc gccaccggcg cctgcggaaa
      721 ttcgatgcgc acgtcgagat ttttcagctt ttcgtcacgg atgttcgaca tgccggcaaa
      781 gcgcagcaag cggcgcgtgg agcgatcgta ggtaacgtcg atatgcgggg cgaggaaggc
      841 gtaccacgcg ccgagcgcaa ggcgcagggt caggcgtgtc gcgtcgctgg cggcatcgac
      901 ccggctaagg tcgaacgcat agaacgtgcg tttgctcggc acgaggaact tgaaacgcaa
      961 cgcgtcgcca tgcgccagcg catcccaatg ccggcgcacg taagcgtcga agccggcgtc
     1021 gatcacgcca tccggcggca gatcgagcgg cgccgatttc tgcgcttggt ccggtgcgcg
     1081 ttgcacgaat atctcgcgcg tcttgccgtt gccacgcacg ccttcgcgat aaccccagcg
     1141 cgcgtccagc atggaaaaat ccggcgcctg cgca
//
LOCUS       DASRVO010000177         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_57018, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000177 DASRVO010000000
VERSION     DASRVO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57018"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFF05_02080"
     CDS             <1..307
                     /locus_tag="VFF05_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZW50625.1"
                     /translation="EGMRQNELAEQLEMEPIAIGRVIDRLQKAGFVERRADPADRRAW
                     RLYLTPRAHSMVGDMEQISSELFHQAQRGISAADMKAMMGMLTRMKDNLNALDESGA"
     gene            322..1443
                     /locus_tag="VFF05_02085"
     CDS             322..1443
                     /locus_tag="VFF05_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HZW50626.1"
                     /translation="MTDKQYGAPATGAPPPAKRKRRNTVLWILGPALVAIIGGWLYLT
                     SGRYVSTDNAYVQADRATIAPQVAGRVVEVDVRENQVVKKGDVLFRIDAEPLQIAVAR
                     AQAQMEGISSLLDAARAGFRSAQANVRSADEALRVNEAQYKRMQELRRKGLVAQKDVD
                     DAANNLADARGKRDADIAAVSKAQSLLGGLPETPNAELSGYKLAKAQLAAAELDLDHA
                     TVRAPIDGTIGKQNLQPGDFLAPGQAAMPLVATKGLWIDANFKETDLTHVRAGQPVDI
                     EVDTYPGHAWKGHVASISPASGAEFSVLPAQNATGNWVKIVQRISVRIAVDDEDSDQI
                     LRAGMSAVVKIDTGKQNSLLGRIEGARAEPESRVAVSAH"
     gene            1452..2978
                     /locus_tag="VFF05_02090"
     CDS             1452..2978
                     /locus_tag="VFF05_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZW50627.1"
                     /translation="MEHAASHREVENRGLLVVSVMLATLMQALDTTIANVALPNMQGS
                     LSATQDEIAWVLTSYIVAAAIATPASGWLASVLGRRRLLLIAVAGFTVASILCGIATT
                     IDQMVLFRLLQGLFGAALVPVSQSTLLDVFPREKHGAAMAMWGMGVMVGPILGPPLGG
                     WLTDNYSWHWVFLINVPIGALCLFGVAASLPRDDTFASRFDWRGFAFLAIGIGALQMM
                     LDRGQLKDWFDSGEIRIEAALTFLGFYLYAVHWLSARRPFVDLGLFRDRNFLASNIFI
                     FLIGIVLFATMALLPPYLQTLMNYPVVTVGVLLAPRGIGTLIAMVIVGRLLARGADAR
                     IVAGIGMLLTALSMWQMTQWTSDVPQWDIIFAGIVQGIGVGLVFVPVSTVAYSTLPMA
                     TRTEAAGIYSLTRNIGSSVGISIMMALLAHNTQVNHAELAARLTPFGTAILPHMSGLT
                     AAASLEALNGEVTRQAATISYLDDFWLMMLLTLAAIPLLALLRPVRHAAPADPALALE
                     "
     gene            2975..3784
                     /locus_tag="VFF05_02095"
     CDS             2975..3784
                     /locus_tag="VFF05_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HZW50628.1"
                     /translation="MNEQIPDRTAPPRRNADEMRVYGINACLALFAKRPHDLRKVYLL
                     ESRIPQFKAVLAWCVQKKLGYRVVGNTDLEKLTASQHHEGVCFEARKIPLPSLTALLQ
                     RLPRAPQPALLAWLDGVGNPHNIGAVLRSAANFGAAGVLLSADAAQGLSGAACRVAEG
                     GAEAVPMARIAPGEDALGTLRNAGFTLAATVPHEGEALFASRLPPRLVFALGAEGEGL
                     SRALIDRADLHLSIPGTGAVESLNVAASAAVLFGEYWRQRHHAAGARAPRR"
     gene            complement(3795..>4567)
                     /locus_tag="VFF05_02100"
     CDS             complement(3795..>4567)
                     /locus_tag="VFF05_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50629.1"
                     /translation="PDLTVAKTHADPFISPSSGDTYTLTVSNGGSDSTSGTVAVADAL
                     PSGLTATAINGSGWSCTMGTLTCMRSDALATGSSYPPVTLTVDVDPSLSGTLVNSAAV
                     SGGGEANTGNDSADDPTTVQPPPDLTIAKTHSGNFTNGQVGATFTITVSNVGSGPTSG
                     TVSVVDNLPAGLTATAISGAGWSCVLGTLTCTRSDVLAAAPAAYPPITVTVDVAANPP
                     AQVINSADVSGGGDGSINNNHADDGTTLPVRLQSFKVD"
BASE COUNT          737 a         1526 c         1522 g          782 t
ORIGIN      
        1 cgagggcatg cgccagaacg agctggccga acagctcgag atggagccga tcgccatcgg
       61 ccgcgtcatc gaccgcctgc aaaaagccgg tttcgtcgaa cgccgtgccg atcccgcgga
      121 ccgccgtgcc tggcgtctgt acctgacgcc gcgcgcacac agcatggtcg gcgacatgga
      181 acagatttct tccgaactct tccaccaggc acaacgcgga atttccgccg ccgacatgaa
      241 ggcgatgatg ggcatgctga cgcggatgaa ggacaacttg aatgcgctgg acgaatccgg
      301 cgcctgactg cgggaatgaa catgaccgac aagcaatatg gagcgcctgc cacgggcgct
      361 ccgcccccgg caaagcgcaa gcgccgcaac accgtgctgt ggatcctcgg cccagcgctg
      421 gtggcgatca tcggcggctg gctgtacctg acgtccggac gctacgtctc caccgacaac
      481 gcttacgtgc aggccgatcg cgccaccatc gcgccgcagg tcgcaggtcg cgtcgtcgag
      541 gtcgacgtgc gcgagaacca ggtggtgaag aagggcgacg tgctgttccg catcgatgcc
      601 gagcccttgc agatcgcggt agcgcgcgca caggcgcaga tggaaggcat cagcagcttg
      661 ctcgacgccg cccgtgcggg attccgttcc gcacaggcga acgtgcgttc ggccgacgag
      721 gcgctgcgcg tgaacgaggc acagtacaaa cgcatgcagg agttgcggcg caagggcctg
      781 gtcgcgcaga aggacgtgga cgacgcggcg aacaatctcg ccgacgcgcg cggcaaacgc
      841 gatgccgaca tcgctgccgt gtccaaggcg cagagcctgc tcggcggcct gccggaaacg
      901 ccgaacgccg aattgtccgg ttacaagctc gccaaggcgc agcttgctgc cgcggaactg
      961 gacctcgatc acgcgaccgt gcgtgcgccg atcgacggca cgatcggcaa gcagaacctg
     1021 cagcccggcg atttcctcgc gcccgggcag gcggcgatgc cgctggtggc gacgaaggga
     1081 ctctggatcg acgcgaattt caaggaaacc gacctcaccc acgtgcgcgc cggccagccg
     1141 gtggacatcg aagtcgacac ctatcccggc catgcctgga agggccacgt cgcctcgatc
     1201 agtccggcct cgggcgcgga gttctcggtg ctgcctgcgc agaacgccac cggcaactgg
     1261 gtcaagatcg tgcagcgcat ttccgtgcgc atcgccgtgg acgacgaaga ttccgaccag
     1321 atcctgcgcg ccggcatgag tgccgtggtg aagatcgaca cgggcaagca gaactcgctg
     1381 cttggccgca tcgagggtgc gcgcgccgaa cccgaatcgc gcgtggcggt ctccgctcac
     1441 tgatcgcgta aatggaacac gctgccagtc atcgcgaggt cgaaaaccgc ggcctgctgg
     1501 tcgtttcggt gatgctggcg accttgatgc aggcgctgga caccaccatc gcgaacgttg
     1561 cattaccgaa catgcaaggt tcgctgtcgg cgacgcagga cgaaatcgcc tgggtgctga
     1621 cctcgtacat cgtcgccgcg gcgatcgcca cgcccgcgag cggctggctg gcgtccgtgc
     1681 ttggccggcg ccgcctgctg ttgatcgccg tggccggttt caccgtggct tcgatcctgt
     1741 gcggcattgc caccacgatc gaccagatgg tgctgttccg cctgttgcag ggtctgttcg
     1801 gcgcggccct ggtgccggtg tcgcagtcga ccttgctcga cgtgttcccg cgcgaaaagc
     1861 acggcgcggc gatggcgatg tggggcatgg gcgtgatggt cggtccgatc ctcggaccgc
     1921 cgctcggcgg ctggctcacc gacaattaca gctggcactg ggtgttcctg atcaacgtgc
     1981 cgatcggcgc gctgtgtctg ttcggcgtgg ctgcgagcct gccgcgcgac gacacctttg
     2041 cctcgcgttt cgactggcgc ggcttcgcct tccttgccat cggcatcggc gcgctgcaga
     2101 tgatgctcga tcgcggccag ctcaaggact ggttcgactc cggcgaaatc cgcatcgagg
     2161 cggcgctcac cttcctcggc ttctatctgt acgccgtgca ctggctaagt gcgcggcggc
     2221 cgttcgtcga cctcggcctg tttcgcgacc gcaatttcct cgccagcaac attttcatct
     2281 tcctcatcgg catcgtgctg ttcgcgacaa tggcgctgct gccgccgtac ctgcagacgc
     2341 tgatgaatta tccggtcgtc accgtcggcg tgctgctcgc gccgcgcggc atcggcaccc
     2401 tgatcgcaat ggtgatcgtc ggacgcctgc tcgcgcgcgg cgcggatgcg cgcatcgtcg
     2461 ccggcatcgg catgctgctc accgcgctgt cgatgtggca gatgacgcag tggacgtccg
     2521 acgtgccgca gtgggacatc attttcgccg gcatcgtgca gggcatcggc gtgggcctgg
     2581 tgttcgtgcc ggtgtccacg gtcgcgtatt cgaccctgcc gatggcaacg cgcaccgagg
     2641 ccgcgggcat ctacagcctg acccgcaaca tcggctcgag cgtcggcatc tcgatcatga
     2701 tggccctgct cgcgcacaac acgcaggtca accatgccga actcgccgcg cgcctgacgc
     2761 cgttcggtac ggcaatcctc ccgcacatga gcggactgac ggcggcggcc agcctggaag
     2821 cgctcaacgg cgaggtcacg cgccaggccg cgacgatttc ctacctcgac gatttctggc
     2881 tgatgatgct cctgaccctg gctgcgattc cgctgctggc cctgctgcgg ccggtcaggc
     2941 atgctgcgcc ggccgatccc gcgctggccc tcgaatgaac gaacaaatcc ccgatcgcac
     3001 ggcgccgccg cgccgcaatg ccgacgagat gcgcgtgtac ggcatcaatg cctgcctcgc
     3061 cctattcgcc aaacgcccgc acgatctgcg caaggtgtac ctgctcgaat cgcgtatccc
     3121 gcaattcaag gcggtgctcg cctggtgcgt gcagaaaaaa ctcggctatc gcgtggtcgg
     3181 caacacggac ctggaaaaac tcaccgccag ccagcaccac gaaggcgtgt gtttcgaggc
     3241 gcgcaagatc ccgctgccgt cgctgacggc gttgttgcaa cgcctgccgc gcgcgccgca
     3301 gcccgcgctg ctggcatggc tcgatggcgt gggcaatccg cacaacatcg gcgcggtgct
     3361 gcgcagcgcg gcgaatttcg gcgcggccgg cgtgttgctt tcggccgatg ccgctcaggg
     3421 cctttccggc gccgcttgcc gcgttgccga aggcggcgcc gaggccgtgc cgatggcgcg
     3481 catcgcgccg ggcgaggatg cgctcggcac attgcgcaat gcggggttca cgctcgccgc
     3541 caccgtgccg cacgaaggcg aagcgctgtt cgcgtcgcgc ctgccgccgc ggctggtgtt
     3601 cgcgttgggc gcggaaggcg aaggcctgag ccgcgccttg atcgaccgcg cggacttgca
     3661 cctttccatt cccggcaccg gcgcggtgga aagcctcaac gtcgccgcca gcgccgccgt
     3721 gttgttcggc gaatactggc gccagcgcca tcacgccgcg ggcgcgcgcg caccgcggcg
     3781 ttgacgcgtc ccgttcagtc gaccttgaac gactgcaatc gcaccggcaa cgtggttccg
     3841 tcgtcggcgt ggttgttgtt gatactgcca tcgccgccgc cggacacatc ggcggaattg
     3901 atcacttgcg ccggtggatt ggcggcaaca tccaccgtta ccgtgattgg cggataggcg
     3961 gccggcgccg cggccagcac atcgctgcgc gtgcaggtca gcgtgccgag gacgcaggac
     4021 catcccgcac cactgatggc cgtggccgtg aggcccgccg gcaggttgtc gaccacgctc
     4081 accgtgccgc tggtcgggcc gctgccgaca ttgctgaccg tgatcgtgaa ggtggcgccc
     4141 acctggccgt tggtaaagtt gccggagtgc gtcttggcga tcgtcagatc gggcggcggc
     4201 tgcaccgtgg tcggatcgtc ggcgctgtcg ttgccggtgt tcgcctcgcc gccgccggaa
     4261 acggccgcgg aattgacgag cgttccggaa aggctcggat cgacgtccac ggtcagcgtg
     4321 accggtggat aggaagagcc agtggccaat gcgtcgctgc gcatgcacgt cagcgtgccc
     4381 attgtgcagc tccagccgct gccgtttatc gctgtggcgg tcaggccgga cggcaaagca
     4441 tcggccacag ccaccgtgcc ggaggtggaa tcgctgccgc cattgctgac cgtgagcgtg
     4501 taggtatcgc cactggatgg cgagatgaag ggatcggcat gcgtcttggc aactgtcagg
     4561 tcgggca
//
LOCUS       DASRVO010000178         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_291158, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000178 DASRVO010000000
VERSION     DASRVO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291158"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFF05_02105"
     CDS             complement(<1..482)
                     /locus_tag="VFF05_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HZW50630.1"
                     /translation="MKIRILSATILVTTLPLASKVLAQDANASAADTPYSVTGNVAIV
                     SDYMFRGITQTWGHPAIQGGGDLTLKNGFAAGLWASRISERSYPGGAMELDLYGSYGA
                     NFNDDWSWHAGLYGYLYPSGNLDQARPRLASRSFNTLEANAALTWKWLTLKYSRSLTD
                     "
     gene            complement(500..2575)
                     /gene="kdpB"
                     /locus_tag="VFF05_02110"
     CDS             complement(500..2575)
                     /gene="kdpB"
                     /locus_tag="VFF05_02110"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZW50631.1"
                     /translation="MTTHHHPQTSALHPKLLMPAIGDAFRKLSPRVQFRNPVMFVVFV
                     CSILTTLLWFQALAGKGEAPAGFILAVALWLWFTLLFANFAEAIAEGRGKAQAASLRN
                     ARRDVVANKIPSLDRREQVTVGSAHDLRKNDLVIVKAGEFIPGDGDVIEGAATVDESA
                     VTGESAPVIRESGGDRSSVTGGTRVLSDWVIVRIGSNPGESFLDRMIAMVEGASRRKT
                     PNEIALTILLAKFTLIFLLACATLLPYSLYSVQAAGHGEPVTITVLVALLVCLIPTTI
                     GGLLSAIGIAGMDRMIQANVIAMSGRAVEAAGDVDVLLLDKTGTITLGNRQAVAFHPA
                     SGVDVKMLADAAQLASLADETPEGRSIVVLAKEKFGIREREVGSAQMQFVPFSASTRM
                     SGVDLIARTIRKGALDAMKKHLREFGSTVPTEVHRAAEDIARKGGTPLVVSENARALG
                     VIELKDIVKGGIRERFAELRAMGIRTIMITGDNPLTAASIAAEAGVDDYLAEATPEAK
                     LKLIRDIQAEGRLVAMCGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTK
                     LIEVVEIGKQMLITRGALTTFSISNDVAKYFAIIPAAFASTYPSLNTLNVMHLATPRS
                     AILSAVIFNALIIIFLIPLALKGVRSRALGAGALLRRNLLIYGIGGMLVPFIGIKLID
                     LLLVLFGLA"
     gene            complement(2601..>2829)
                     /locus_tag="VFF05_02115"
     CDS             complement(2601..>2829)
                     /locus_tag="VFF05_02115"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HZW50632.1"
                     /translation="ICMFIARFPLALGMLAIAGSLAAKKHVPASAGTLPTHTPLFVVL
                     LAITVVIVGALTFLPALALGPIAEHLAMLGR"
BASE COUNT          505 a          882 c          941 g          501 t
ORIGIN      
        1 taatccgtca gcgaacgcga gtacttgagc gtgagccact tccaggtcag cgcggcattg
       61 gcttcgagcg tattgaacga gcgtgaagca aggcgcggcc tggcctgatc gagattgccg
      121 ctcgggtaga ggtagccgta caacccggca tgccacgacc agtcgtcgtt gaagttcgcg
      181 ccgtagctgc cgtacagatc gagctccatc gcgccgccgg gatagctgcg ctcgctgatt
      241 cttgatgccc acaagccggc tgcgaagccg ttcttcagag tcaggtcgcc accgccctgg
      301 atcgccggat ggccccaggt ttgcgtaatg ccgcggaaca tgtagtccga gacaatggca
      361 acattgccag tgactgaata cggcgtatcc gcggcggatg cattggcatc ctgcgccaac
      421 accttgctcg ccagcggaag cgtggtcacg agaatggtcg ccgaaagaat gcgaatcttc
      481 atgaatgtct ccgtaaatct cacgctagac cgaacagcac cagcagaagg tcgatcagct
      541 tgatgccgat gaacggcacg agcatgccgc cgatgccgta gatcaacaga ttgcggcgca
      601 gcagcgcgcc ggcgccgagc gcgcgcgagc gcacgccctt gagcgcgagc ggaatcagga
      661 aaatgatgat caacgcgttg aagatcaccg ccgacaggat cgcactgcgc ggcgtggcga
      721 ggtgcatcac gttgagggtg ttgagagaag gataggtgct cgcaaaggcg gccggtatga
      781 tcgcgaaata cttggccacg tcgtttgaga tcgaaaacgt ggtcagcgcg ccgcgcgtga
      841 tcagcatctg cttgccgatc tcgaccactt cgatcagctt ggtcggattg gaatcgaggt
      901 cgaccatgtt gccggcttcc ttcgccgcct gcgtgccgga gttcatcgcc actgcgacgt
      961 cggcctgcgc cagcgccggc gcgtcgttgg tgccgtcgcc gcacatggcg acgaggcggc
     1021 cttccgcctg gatgtcgcgg atcaacttga gctttgcttc cggcgtggcc tcggcgaggt
     1081 agtcgtccac gccggcttcc gcggcaatcg acgcggccgt gagcgggttg tcgccggtga
     1141 tcatgatggt gcggatgccc atcgcgcgca gctcggcgaa gcgctcgcgg atgccgccct
     1201 tgacgatgtc cttgagctcg atcacgccaa gcgcgcgggc gttctcgctc accacgagcg
     1261 gtgtgccgcc cttgcgcgcg atgtcctcgg cggcgcgatg tacttccgtg ggaaccgtcg
     1321 agccaaattc gcgcaggtgt tttttcatcg cgtcgagcgc gcccttgcgg atggtgcgcg
     1381 cgatgagatc cacgccgctc atccgagtcg aagccgagaa cggcacgaac tgcatctgcg
     1441 cgctgccgac ctcgcgttcg cgaatgccga atttttcctt ggccagcacg acgatgctgc
     1501 ggccttccgg cgtctcgtcg gcgagcgagg cgagttgcgc ggcatcggcc agcattttca
     1561 cgtccacgcc gctggccgga tggaatgcaa ccgcctggcg gttgccaagc gtgatcgtgc
     1621 cggtcttgtc gagcagcagc acgtcgacgt cgccggcggc ttccaccgcg cggcccgaca
     1681 tcgcgatcac gttcgcctgg atcatgcggt ccatgccggc gatgccgatg gcggagagca
     1741 gtccaccgat cgtggtcggt atcaggcaaa ccagcagcgc gaccagcacc gtgatcgtga
     1801 cgggttcgcc gtgaccggcg gcctgcacgc tgtacagcga gtacggcagc agcgtggcgc
     1861 aggcgagcag gaagatcagc gtgaacttgg ccagcaggat ggtgagcgcg atttcgttcg
     1921 gcgtcttgcg ccgcgaggca ccttcgacca tcgcgatcat gcggtcgagg aagctttcgc
     1981 ccggattgct gccgatgcgc acaatcaccc agtccgacag cacgcgcgtg ccgcccgtga
     2041 ccgaactgcg gtcgccgccg gattcgcgga tcaccggggc ggactcgccg gtcaccgcgc
     2101 tttcgtcgac ggttgcggcg ccttcgatca cgtcgccgtc gccgggtatg aactcgccgg
     2161 ctttcacgat cacgaggtcg ttcttgcgca agtcgtgtgc ggaccccacc gtaacttgtt
     2221 cgcgtctgtc gagagaggga atcttgttgg ccaccacatc gcgccttgcg ttgcgcagcg
     2281 acgctgcttg cgccttgccg cgaccctcgg cgatggcttc ggcgaagttc gcgaacagca
     2341 gcgtgaacca cagccacagc gcgacggcga ggatgaagcc cgccggcgct tcgccttttc
     2401 cggccagcgc ctggaaccac agcagcgtgg tcaggatgct gcagacgaac accacgaaca
     2461 tcaccgggtt gcggaattgc acgcgcggcg acagcttgcg gaacgcatcg ccgatcgcgg
     2521 gcatcagcag cttcggatgg agcgcgctgg tttggggatg atgatgagtc gtcatggttg
     2581 tctcggtcag tcgatgccgg tcagcgaccg agcatggcca ggtgttcggc gatcgggccc
     2641 aatgccagcg ccggcaggaa ggtgagcgca ccgacgatca cgacagtgat cgcgagcaac
     2701 acgacgaaca gcggcgtatg cgtgggcagc gtgccggcgg aggcaggcac gtgcttcttc
     2761 gccgcgagcg agccggcgat cgcgagcatg ccgagcgcga gcgggaagcg cgcaatgaac
     2821 atgcagatg
//
LOCUS       DASRVO010000179         1336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_316873, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000179 DASRVO010000000
VERSION     DASRVO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316873"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VFF05_02120"
     CDS             <1..889
                     /locus_tag="VFF05_02120"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZW50633.1"
                     /translation="IVNSHAGSVQHPELRDRISAICAESGAEARIITSDAGQAVPLLA
                     RQALDSADVVVAVGGDGTINAVASVLAGSGVPLGVLPFGTLNHFAKDARIPLDLDSAV
                     RNAIAGTAAHIDVGEVNGRIFLNNSSLGLYPRMVEQRTEEQQRGASKWPAFARAMLSV
                     LWRYPPLRAQIVADQTTLSRKTPFIFIGNNHYDVEGRRVGSRHRLDAGELSLITARPV
                     SRSALVWSAVRTLFQGPQPGSAFDYLTAHEIVVRVRRRRRVLVALDGEVMQLEPLLHY
                     RIRPGALRVMLPAAAESAA"
     gene            893..>1336
                     /locus_tag="VFF05_02125"
     CDS             893..>1336
                     /locus_tag="VFF05_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZW50634.1"
                     /translation="MRTIAHLSDLHFGRSDAAVADALSVHVRSLKPDVVVISGDLTQR
                     AREREYQQAADFLRLLPSPQIIVPGNHDVPLHNVVARLRSPLDFYRRYISDDLAPYYR
                     DEEIAVLGINTARSLTWKNGRISREQVIASCEQLERSTGDVVRMIV"
BASE COUNT          215 a          477 c          384 g          260 t
ORIGIN      
        1 catcgttaac agccacgccg gttcggtcca gcatcccgag ctgcgcgacc gcatcagtgc
       61 catttgtgcg gagagcggcg cggaagcccg catcatcacc tccgatgccg gtcaagccgt
      121 gccgctgctc gcgcgtcagg cgctcgattc cgccgacgtc gtagtcgcgg tcggcggtga
      181 cggcaccatc aacgccgtgg catcggttct cgccggaagc ggcgttccac tcggcgtgct
      241 cccctttgga accctgaatc acttcgccaa ggatgcccgc atccccctcg acctcgactc
      301 cgccgttcgc aacgccattg ccggaactgc ggcccacatc gatgttggcg aagtgaatgg
      361 gcgcattttc ctgaacaact cgagtctcgg tctctatccg cggatggtcg aacagcgtac
      421 cgaagagcaa cagcgcggcg cttccaaatg gccggccttc gcgcgtgcca tgctgtcggt
      481 cttgtggcgt tatccgcctt tgcgtgcgca gatcgtcgcc gaccaaacca ccctgtcgcg
      541 gaagacgccg ttcatcttca tcggcaacaa tcattacgac gtcgaagggc gccgcgtcgg
      601 ctcgcgccat cgcctggatg ccggcgaact gtcgctgatt accgcgcgcc ccgtgtcgcg
      661 cagcgcgctg gtatggtcgg ctgtgcgtac gctgttccag gggccgcagc ccggcagtgc
      721 attcgattac ctcacagcgc acgaaatcgt cgttcgcgtt cgccgccgcc gtcgtgtgct
      781 cgtggccctt gatggcgaag tcatgcagct tgagccgctg ttgcactatc gcatccgccc
      841 tggcgctctg cgggtcatgc ttcccgccgc cgcagaaagt gctgcctgac cgatgcgcac
      901 catcgcccat ctttccgatc tgcacttcgg ccgctccgac gccgccgtgg ccgacgcgct
      961 ttccgtgcac gtccgtagcc tcaagcccga tgtcgtggtc atctccggcg acctcaccca
     1021 gcgtgcccgc gaacgcgaat accagcaggc cgccgacttc cttcgcctgc tgccgtcccc
     1081 gcaaatcatt gtccccggca accacgacgt gcccctgcac aatgtggtcg cgcgccttcg
     1141 ctcgccgctt gatttttatc gtcgctacat cagcgacgat ctcgcgcctt actatcgcga
     1201 cgaagagatc gctgtcctgg gcatcaacac ggcgcgatcg ctcacgtgga agaacggccg
     1261 catcagccgc gagcaggtaa tcgcatcatg cgaacagctc gagcgcagca cgggcgacgt
     1321 cgtccggatg atcgtt
//
LOCUS       DASRVO010000180         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_291199, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000180 DASRVO010000000
VERSION     DASRVO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291199"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFF05_02130"
     CDS             <1..442
                     /locus_tag="VFF05_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZW50635.1"
                     /translation="KPVETPPPARGKPPENVAELNAGGVAWRWPADGKVVSTYVSGDQ
                     TKQGLDIAGNAGEAVRAAADGEVVYSGNGLIGYGELIIVKHNANFLSAYGHNRKRLVK
                     EGERVKAGQEIAEMGSTSASRDELHFEIRKNGKPVNPLEYLPAR"
     gene            515..883
                     /locus_tag="VFF05_02135"
     CDS             515..883
                     /locus_tag="VFF05_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50636.1"
                     /translation="MKAKLAFVAGLVVSLGGCAVEQYRPLTDSGVSKGSYEADLADCQ
                     QLAAQRPAAQQAAGGAAVGAVLGAVLAAAAGLRGNDVARVAAWGAANGGVQGAVYGSA
                     EQRDIVDRCMAGRGYNVVAP"
     gene            963..1844
                     /locus_tag="VFF05_02140"
     CDS             963..1844
                     /locus_tag="VFF05_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZW50637.1"
                     /translation="MTAPDDDLDAEELLVLLDSVEPRDDAARDSASAYLGEIGLIPLL
                     DAAGERALAQRVRAGEAQARQEMIEANLRLVVSVARGYVGRGVPLMDLIAEGNLGLIR
                     AVEKFEPARRLRFSTYAVWWIREAVQHALMHQGRTVRVPVHVLREYARVLRARREFVA
                     AHGRVPGVDELAQAVGKSVTEVSELLAATERISSLDAPLSESDDRALSEQISLNDAET
                     ANADSAPSAGLGAWIVQLPERERWVLERRFGLNDHGVQTLAEVAEQLGLTRERVRQIQ
                     AAALKKLRALMERDDAD"
     gene            complement(1841..2215)
                     /locus_tag="VFF05_02145"
     CDS             complement(1841..2215)
                     /locus_tag="VFF05_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HZW50638.1"
                     /translation="MQLTEHRYEQQFFLRHADAQSVTVVDCVLHRSLAVSATRIVEDF
                     PVQRVDQFDDAAIESILAFNPEVVLLGTGARAVFPPQAVLAQFLKRGIGLETMDSAAA
                     ARTFNVLAGEGRNVVAVFLLPN"
     gene            complement(2219..2512)
                     /locus_tag="VFF05_02150"
     CDS             complement(2219..2512)
                     /locus_tag="VFF05_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HZW50639.1"
                     /translation="MPITPSQKRYLRGLAHSLKPVILTGNKGVTPALLKEFSAALDQH
                     ELVKVKLGDDRDERKSHIAELGEGAKAELVQSIGRVACFYRHNEEKPKLALPR"
     gene            2546..3193
                     /gene="rlmE"
                     /locus_tag="VFF05_02155"
     CDS             2546..3193
                     /gene="rlmE"
                     /locus_tag="VFF05_02155"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HZW50640.1"
                     /translation="MRLCSISRRRVARSKSSSRWLKEHFSDPFVKRAQAEGWRSRAVF
                     KLEELIERDHLLKPGMVVVDLGAAPGGWSQMVRERLGDAGRIVALDILPMQGIAGVEF
                     IEGDFREDEVLQRLEATLHGAPVDLVLSDIAPNMSGVGVADQARAMHLAELAQEFAAA
                     HLKPGGVFVVKLFQGRGFDEYVRNLRKAYERVSMRKPKASRARSPEVYALATGKK"
     gene            3202..>3582
                     /locus_tag="VFF05_02160"
     CDS             3202..>3582
                     /locus_tag="VFF05_02160"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HZW50641.1"
                     /translation="MNDMAKNLLLWIVIAVVLIAVFQSFNPRSTSPRNISYSDFMQQV
                     ENNSVAQVSISATMPSNITGKRKDGSQLSTTAPFDDSQMIPTLRSHNVEVSQEPVDNS
                     GVFWRALIEWVPILLFFGVIIYFMR"
BASE COUNT          626 a         1090 c         1248 g          618 t
ORIGIN      
        1 gaagccggtc gaaactccgc cgccagcgcg ggggaagccg cccgaaaatg ttgccgaact
       61 caatgccggt ggcgtcgcct ggcgctggcc cgccgacggc aaggttgtca gtacctacgt
      121 ttccggcgat cagacgaagc agggtctcga catcgccggc aacgcgggcg aagcggtgcg
      181 cgccgccgcc gacggcgagg tggtgtacag cggcaacggc ctgatcggtt acggcgaact
      241 catcatcgtc aagcacaacg cgaacttcct gtccgcctac ggccacaacc gcaagcgcct
      301 ggtcaaggag ggcgagcgcg tgaaagccgg ccaggaaatc gccgagatgg gttcgaccag
      361 cgccagccgc gacgaactgc acttcgagat acgcaagaac ggcaagccgg tcaatccgct
      421 ggaatacctg ccggcgcggt aactaccatt gcgatccgtg agatcgccgc gtcgcaggct
      481 gtagactgtg gccgtgggga aaatggggaa atccatgaaa gcgaaactcg ccttcgtcgc
      541 gggtctggtc gtgtcgcttg gcggttgcgc ggtcgagcag taccggccat tgaccgattc
      601 cggcgtcagc aagggcagtt acgaggctga tctcgccgat tgccagcaac tggcggcgca
      661 gcgtccggcc gcgcagcaag cagccggcgg agccgccgtc ggcgcggtgc tcggtgcggt
      721 cttggccgca gcggcaggac tgcgcggcaa cgacgtcgcg cgcgtagccg cgtggggcgc
      781 ggccaacggc ggcgtgcaag gcgccgttta tggttccgcc gaacagcgcg acatcgtgga
      841 tcgctgcatg gccggtcgcg ggtacaacgt ggtggcaccg tagccgatac gctcgaacaa
      901 gaaggcgctc aacgcgctcc gccgacgcgg cctgcccacg cggcgcggtg tatcctgcga
      961 ccatgaccgc gcccgacgat gatctcgacg ccgaggaact gctcgtgctg ctcgacagcg
     1021 tggagccgcg tgacgacgcg gcgcgcgatt cggcgagcgc gtacctgggt gagattggcc
     1081 tgattccgct gctcgacgcc gcgggcgagc gcgcgcttgc gcagcgcgta cgcgccggcg
     1141 aggcgcaggc gcgacaggag atgatcgagg cgaacctgcg cctggtcgtc agcgtggcgc
     1201 gcggctacgt cggccgcggc gtgccgctga tggacctgat cgccgagggc aacctggggc
     1261 ttatccgcgc ggtggaaaag ttcgaaccgg cgcggcgcct gcgtttttcc acctacgcgg
     1321 tgtggtggat ccgcgaggcc gtgcagcacg cgctgatgca ccagggccgc accgtgcgcg
     1381 tgccggtgca cgtgctgcgc gagtacgcgc gcgtgctgcg cgcgcggcgc gagttcgtgg
     1441 ccgcgcacgg acgcgtgccc ggcgtcgatg agctcgcgca ggcggtcggg aaatccgtga
     1501 ccgaggtgag cgagctgctt gccgcgaccg aacgcatcag ctcgctggat gcgccgttgt
     1561 cggaaagcga cgatcgcgca ctgagcgaac agatttcgtt gaacgatgcg gaaaccgcaa
     1621 acgccgacag cgcgccatcc gccggtcttg gcgcgtggat cgtgcaactg cccgagcgcg
     1681 agcgctgggt gctcgaacgt cgctttggcc tgaacgatca cggcgtgcag acgctggcgg
     1741 aagtcgccga acaactcggc ctgacccgcg agcgcgtgcg ccagatccag gcggcggcgc
     1801 ttaagaagtt gcgtgctctg atggaacgcg acgatgcgga ttagttcggg agcagaaaca
     1861 ccgccaccac attccgccct tcgccagcca gcacgttgaa cgtacgcgcc gcagcggcgc
     1921 tgtccatggt ttccaggccg atgccgcgtt tgaggaattg cgcgagcacg gcctgtggcg
     1981 gaaacacggc gcgcgcgccg gtgccgagca gcacgacctc gggattgaag gcgaggatgg
     2041 attcaatcgc ggcgtcgtcg aattgatcaa cgcgttgcac cggaaaatcc tcgacgatgc
     2101 gcgtggcgct tacggccagg ctgcgatgta gtacgcagtc gactacggtg acggattgcg
     2161 catccgcatg gcgaaggaag aattgctgtt cgtagcggtg ttcggttagt tgcatggatt
     2221 atctgggcag cgccagcttc ggcttttctt cgttgtgccg gtagaagcag gcgacgcggc
     2281 cgatgctttg cacgagttcg gccttggcgc cttcgccgag ttcggcgatg tgcgacttgc
     2341 gttcgtcgcg atcgtcgccg agcttgacct tgaccagttc gtgctggtcg agcgcggcgg
     2401 aaaattcctt gagcagggcc ggcgtgacgc ctttgttgcc ggtcaggatc accggtttga
     2461 ggctgtgcgc gaggccgcgc agatagcgtt tctggctggg cgtgatcggc atggaggcga
     2521 cccgttctgg gaattacagg gtatcatgcg gctgtgcagc atttcgaggc gtcgcgtggc
     2581 acgtagcaag tccagttcgc gctggctcaa ggagcatttc tccgatccct tcgtcaaacg
     2641 cgcgcaggcc gaaggctggc gttcgcgcgc ggtgttcaag ctcgaggagt tgatcgagcg
     2701 cgaccacttg ctcaagccgg gcatggttgt ggtggatctg ggcgcggcgc cgggcggctg
     2761 gtcgcagatg gtgcgcgagc gcctcggcga cgccggccgc atcgtggcgc tggacatcct
     2821 gccgatgcag ggcatcgccg gggtggagtt catcgagggc gatttccgcg aggacgaggt
     2881 tctgcaacgg ctggaagcga cgttgcacgg cgccccggtc gaccttgtgc tttcggacat
     2941 cgcgcccaat atgagcggtg tgggcgtggc tgatcaggca cgcgccatgc atctggctga
     3001 attggcacag gaattcgccg ccgcccacct gaaacccggc ggcgtgttcg tggtcaaatt
     3061 atttcagggc cgcggtttcg acgagtacgt gcgaaacttg cgcaaggctt acgagcgcgt
     3121 cagcatgcgc aaaccgaagg cctcacgcgc gcgcagcccc gaagtttacg cgttggcgac
     3181 cggcaagaag tgagagacac catgaacgat atggccaaga acctgctgct ctggatcgtg
     3241 atcgcggtcg tactgatcgc cgtgttccag agtttcaatc cgcgcagcac gagtccgcgc
     3301 aacatttcct attcggactt catgcagcag gtcgagaaca attcagtcgc gcaggtgtcg
     3361 atcagcgcca cgatgccgtc caacatcacc ggcaagcgca aggacggcag ccagttgtcg
     3421 accaccgcgc cgttcgacga cagccagatg atcccgaccc tgcgctcgca caatgtcgag
     3481 gtcagccagg agccggtgga caacagcggg gtgttctggc gcgccttgat cgaatgggtt
     3541 ccgatcctgt tgttcttcgg cgtcatcatc tatttcatgc gc
//
LOCUS       DASRVO010000181         1470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_108884, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000181 DASRVO010000000
VERSION     DASRVO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108884"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFF05_02165"
     CDS             <1..181
                     /locus_tag="VFF05_02165"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971794.1"
                     /note="converts protoheme IX and farnesyl diphosphate to
                     heme O; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HZW50642.1"
                     /translation="AGFLYYAVKLLNPPDERFAMRVFNYSIVYLMALFAFMLIDHYID
                     SSPLQRTILTLHRIG"
     gene            222..701
                     /locus_tag="VFF05_02170"
     CDS             222..701
                     /locus_tag="VFF05_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZW50643.1"
                     /translation="MTSVYDFSARTIDGDEQKLSAYKGKTLLVVNVASKCGFTPQYTG
                     LEALYEKFKDKGLVVLGFPCDQFGHQEPGDENEIKNFCSLTYDVKFPLFAKIEVNGAN
                     THPLYKYLKAQAKGLLGSESIKWNFTKFLVDADGKVVTRYAPTDTPEKIEKDVARML"
     gene            698..>1470
                     /locus_tag="VFF05_02175"
     CDS             698..>1470
                     /locus_tag="VFF05_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007963964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZW50644.1"
                     /translation="MSKAAADQFDAILVGGGHNGLTCAAYLARAGLNVLVLEKRSVVG
                     GAAVTEEFHPGFRNSVAAYTVSLLQPKVIRDLDLAAHGLKIVLRRANNFLPRPDGSYL
                     LTGEGRTRAEIAKFSQKDAEHLPEYERRLDAIADLLRDLALQPPPNVTDGSWLKALPE
                     LLRAGRLGKRMKSLDATLRTDLLDLFAISAAEYLDRFFESEPIKAVLGFDGIVGNFAS
                     PYTPGSAYVLLHHVFGEVNGVKGAWGHAIGGMGAITQAMA"
BASE COUNT          288 a          477 c          445 g          260 t
ORIGIN      
        1 cgcgggattc ctgtactacg ccgtgaagtt gctgaacccg ccggatgagc gctttgccat
       61 gcgcgtgttc aactattcca tcgtctacct gatggcgctg ttcgcgttca tgctgatcga
      121 ccactacatt gattcgtctc cgctccagcg cacgatcctg acgctgcacc ggatcgggtg
      181 acgcgctaca cttcggcacc gattcgcacc ggagcggcgc catgaccagc gtgtacgatt
      241 tttccgccag gaccatcgac ggcgacgagc agaaattgtc cgcctacaag ggcaagaccc
      301 tgctcgtggt gaacgtcgcg agcaaatgcg gcttcacgcc acagtacacg ggcctggaag
      361 cgttgtacga gaagttcaag gacaagggcc tggtcgtgct cggctttccc tgcgaccagt
      421 tcggccacca ggaaccgggc gacgaaaacg agatcaagaa tttctgctcg ctaacctacg
      481 acgtgaaatt cccgctgttc gccaagatcg aggtcaacgg cgcgaacacc catccgctgt
      541 acaaatacct gaaggcgcag gccaagggac tgctcggcag cgaatcgatc aagtggaatt
      601 tcaccaagtt cctggtcgac gcggatggca aggtggttac gcgctatgca cccaccgata
      661 cgccggaaaa gatcgaaaaa gacgtggcgc gcatgctgtg agcaaggcgg ccgcggacca
      721 attcgacgcc atcctcgtcg gcggcggcca caacggactg acttgcgcag cctacctcgc
      781 gcgcgccggc ctgaacgtgc tggtgctgga aaagcgcagc gtggtcggcg gcgccgcggt
      841 gaccgaggaa ttccatccgg gcttccgcaa ttcggtcgcg gcctacacgg tgtcgctgct
      901 gcaacccaag gtcatccgcg atctcgatct tgccgcgcac ggattgaaaa tcgtcctgcg
      961 ccgcgcgaac aatttcctgc cgcgacccga tggctcgtac ctgctgaccg gcgagggccg
     1021 tacgcgcgcc gaaatcgcca agttttcgca gaaggacgcc gagcacctgc cggaatacga
     1081 gcggcgcctc gacgccatcg ccgacctgct gcgcgatctc gccctgcaac cgccgccgaa
     1141 cgtcaccgac ggtagttggc tcaaggcgct gcccgagcta ctgcgcgccg gacggctcgg
     1201 caagcgcatg aaatcgctgg atgcgaccct gcgcaccgat ctgctcgacc tgttcgcgat
     1261 ttccgccgcc gaatacctcg accgcttctt cgagagcgag ccgatcaagg ctgtgctcgg
     1321 cttcgacggc atcgtcggca atttcgccag tccgtacacg ccgggcagcg cctacgtact
     1381 gttgcaccac gtgtttggcg aggtcaatgg cgtcaagggc gcgtggggcc acgcgatcgg
     1441 cggcatgggc gcgatcacgc aggcgatggc
//
LOCUS       DASRVO010000182         1452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_212974, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000182 DASRVO010000000
VERSION     DASRVO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212974"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFF05_02180"
     CDS             complement(<1..203)
                     /locus_tag="VFF05_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HZW50645.1"
                     /translation="MSAQNLASWLDYQQRIHPRAIELGLDRVREVWRRMGAPAPAPIV
                     ITVGGTNGKGSTVAFLEAMLAASG"
     gene            complement(213..1079)
                     /gene="accD"
                     /locus_tag="VFF05_02185"
     CDS             complement(213..1079)
                     /gene="accD"
                     /locus_tag="VFF05_02185"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZW50646.1"
                     /translation="MSWLQKLMSSRIRTEGTTAKGKVPEGLWEKCAGCGAVLYRPELE
                     RNLEVCPKCGHHHAIGARKRLAALFDEGVGEELFAQLEPVDALKFRDSKKYKDRLVAA
                     QKQTGEKDSLVAMRGLLKTRPLVVCAFEFAFMGGSMASVGGEKFARAAELALDERIPF
                     VCFSASGGMRMQEGLFSLMQMAKVSAALARLRNAGVPYVSVLTHPTTGGVSASLGMLG
                     DLNIGEPKAQIGFAGPRVIEQTVRETLPEGFQRSEFLLDHGAIDLIVDRREMRDKLAD
                     LFALLMRQPKAA"
     gene            complement(1183..>1452)
                     /locus_tag="VFF05_02190"
     CDS             complement(1183..>1452)
                     /locus_tag="VFF05_02190"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZW50647.1"
                     /translation="ANRLSLDAVRERVAAIKVRTPTPVAVGFGVRDAASAAAIARFAD
                     AVVIGSALVEKLAGASSAEAACAAAREFLAPIRAALDAGAQAHAA"
BASE COUNT          246 a          505 c          454 g          247 t
ORIGIN      
        1 cccgacgcgg ccagcatcgc ctcaaggaac gcaacggtcg agcccttgcc gttggtgccg
       61 ccgacggtga tgacgatcgg agcgggcgcc ggtgcgccca tgcggcgcca gacttcgcgc
      121 acgcggtcca gccccagttc gatggcgcgc ggatggatac gttgttggta atccagccag
      181 ctggcgaggt tttgcgcgga cacgtcttgc ggtcaagccg ccttcggctg ccgcatcagc
      241 agcgcgaaca gatcggcgag cttgtcgcgc atctcgcgac ggtcgacgat caggtcgatc
      301 gcgccgtgat cgagcaggaa ttccgaacgc tggaaccctt ccggcaacgt ttcgcgcacg
      361 gtctgctcga tcacgcgcgg cccggcgaat ccgatctgcg ccttgggctc gccaatgttg
      421 aggtcgccga gcatgcccag gctcgccgac acgccgccgg tggtcggatg cgtaagcacc
      481 gacacatacg gaacgccggc attgcgcagg cgcgcgagcg ccgcggaaac cttcgccatc
      541 tgcatcagag agaacaggcc ttcctgcatg cgcatcccgc cgctggcgga gaaacacacg
      601 aacgggatgc gttcgtccag cgccagttcc gccgcgcgcg caaatttttc gccaccgacc
      661 gaggccatcg agccgcccat gaatgcgaat tcgaaggcgc aaacaaccag tggccgcgtc
      721 ttgagcaggc cgcgcatggc gacgagggaa tccttctcgc cggtctgttt ctgcgcggcg
      781 acaagacgat ccttgtattt tttcgagtcg cggaatttca gcgcgtcgac gggttcgagt
      841 tgcgcgaaca attcttcgcc gacaccctcg tcgaacaacg cggccaggcg cttgcgcgcg
      901 ccgatcgcat gatgatgccc gcacttcgga catacttcca ggttgcgctc gagctcgggc
      961 cggtacagca cggcgccgca gccggcgcac ttttcccaca ggccttccgg caccttgccc
     1021 ttggccgtgg tgccctcggt gcggatgcgc gaagacatca atttttgcag ccagctcata
     1081 tcgtctgctt ctcccgtttc cagcggcgcc acggcctcgg ccggcaccgg ttgttccgat
     1141 accgaccgat tcaataccga ctggcccggc ggcaggcgcg gatcacgcgg catgcgcctg
     1201 cgctcccgca tccagcgcgg cacggatcgg cgcgaggaat tcgcgcgccg cagcgcaagc
     1261 cgcttcagcc gaactcgcgc ctgccagttt ttcgaccagc gcgctgccga tcacgaccgc
     1321 atcggcaaac cgcgcgatcg ccgcggcgga tgccgcatcg cgcacaccga atccgactgc
     1381 gaccggcgtc ggcgtgcgta ccttgattgc agcgactcgt tcgcgcacgg catccaggct
     1441 caggcgattg gc
//
LOCUS       DASRVO010000183         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_5323, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000183 DASRVO010000000
VERSION     DASRVO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5323"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VFF05_02195"
     CDS             complement(<1..945)
                     /locus_tag="VFF05_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW50648.1"
                     /translation="MSASSWLGSRTAAAPPRWRELEESARAVRRWKVLHAGSAPHDLQ
                     LLTEAQAKLGMRPSFALSLAVGSTSRPGLLGSWNEVKTWRTQLAASGAGAADVVHAHE
                     FAAGMAALRAGYPLVYAFRAPLEDWREGGFQAGAWLRRSMRVAEQFLLTRAGAVVVHS
                     HAMWNRAVGRGVATENLFLIPDPIGCGGEDADFDWLRRLVGERNSVILYAPGIVLDST
                     VSPEAKILLEAFAFVREELEGARLLLQTRSPGEIGNDGVTAIGAADAERALAAADVVI
                     SFADSEGPDAVALAAMSRGRPLLSADRAGMREVSADGRG"
BASE COUNT          200 a          398 c          312 g          169 t
ORIGIN      
        1 tccccggccg tcggcgctga cttcgcgcat tcccgcgcga tcggctgata gcaaaggccg
       61 cccccggctc atggcagcca gcgccacagc gtcgggacct tcagagtcgg caaaggaaat
      121 caccacgtcg gcagcggcca gcgcccgctc cgcatcggcg gcgccgatcg cagtcacacc
      181 gtcgtttccg atttcgcccg gcgacctggt ttgcagcaac agccgtgctc cttcgagttc
      241 ctcccgcacg aaagcaaacg cttccagcaa aatcttcgcc tcgggagaaa ccgtggagtc
      301 cagcacaata cccggcgcgt acaagatgac cgaattgcgt tcgccgacta accgccgcag
      361 ccagtcgaaa tcggcatctt ctccgccgca cccgatcggg tcgggaatca ggaagagatt
      421 ttcggttgcg accccgcgcc ccaccgcgcg attccacatg gcatgcgagt gcaccaccac
      481 cgcgccggcg cgggtcagca gaaactgttc ggcgactcgc atcgagcgcc tcagccaagc
      541 gcccgcctgg aaacctcctt cacgccagtc ctccaacggc gcgcgaaagg cgtacaccag
      601 cgggtagccg gcacgcagcg ccgccatccc ggcggcgaat tcatgtgcgt gcaccacgtc
      661 ggcggcgccc gcgccgctgg cagccagttg cgttcgccag gtctttactt cgttccacga
      721 tcccaatagt cccggccgcg aagtcgaacc tacagcgagc gacagcgcga acgacggccg
      781 cattcccagc ttggcctgcg cctccgtcaa taactgcagg tcgtgcggag ccgagccggc
      841 atgcaatacc ttccagcggc gaacggcgcg ggcactctcc tcaagctcgc gccaccgcgg
      901 cggcgccgcc gcggttcgag aacccagcca ggaactcgcg ctcacgaagc ctccttacgc
      961 tggctgcgcg tcaggtccgc atacagcacc acctggttgc ggcctttgct cttggcggaa
     1021 tacagcgcgg cgtcggcgaa ttcgatcaaa tcgctcttga actgcgcgtc gctgtcgaa
//
LOCUS       DASRVO010000184         1972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_135040, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000184 DASRVO010000000
VERSION     DASRVO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135040"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="VFF05_02200"
     CDS             complement(<1..1952)
                     /locus_tag="VFF05_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZW50649.1"
                     /translation="MLLRCVRAGQTQLALAALEHGADPNGVPPADDRDQRSALVLAAL
                     SPDTRLLRGFIAKGANLQHAHAGLPLLIAATRDSREGRAEAVMTLLTNGAAVNCNDAD
                     GNTPLHFAALSAKPVVAALLCDAGARLDAINRAGETPLVMACAAGNWELARFLLERGA
                     KPETSRAQPALIAAASIDDDDPDGVKLLLKRKARVDVHDALQRTPLMAAALRGHAVIA
                     RVLLDAGARVDAADAHGTTALMEAARANAHAVLDLLIASRPPPDAVDHAGRSALTVAA
                     QSLCAGEETVRRLLAFGISRQLAAKDGRRAVDFAAAAGRWNIVAAIDPEYPRPAHLQA
                     DKAVELSKDVPDHLLDALRFGHWQIAENFAGEIRDWPPQTVARLFGELIDHSDAAPRR
                     WLLNHGLDANASLDGLPLLRRALLQLPETLAAASDLVDAGAQPTGGDAIAQICIAAMA
                     AGIPQPDLETFALRVLERGAEAFNAGDEGRTPLALALSNGWQRLCAALLARGADVRTR
                     DRRGRTPLFDALLAPEPRRESLIKALLRAGADPEVRAANNETPLGLALARGHADLQRW
                     LNWSAWKLPCRPLRAVDTIAAASAGDAEAVGKLLELGLPVDAVDGYGATALLHAAGQG
                     HAGVVALLLDRGASPTHAAKDGATAL"
BASE COUNT          323 a          697 c          636 g          316 t
ORIGIN      
        1 ctcaacgccg ttgccccgtc cttggcggca tgcgtcgggc tcgcgccgcg atcgagcaac
       61 agcgcaacca cgcccgcatg cccttgaccg gcggcgtgca gcagcgctgt cgcaccatag
      121 ccatcgaccg catccaccgg caatccgagt tcaaggagct tgccgacagc ctcggcatcg
      181 cctgcggatg ccgccgcgat cgtgtcgacc gcgcgcaatg gcctgcacgg cagtttccag
      241 gccgaccagt tcagccagcg ctgcagatcc gcgtgcccgc gcgccagtgc caggcccaac
      301 ggggtttcgt tgttggctgc gcgcacttcg gggtccgcgc ccgcgcgcag cagagccttg
      361 atcaggctct cacgccttgg ttccggcgcc agcagcgcgt cgaacagcgg cgtgcgtccg
      421 cgccgatctc gggttcgcac gtcggcgcca cgcgccagca gcgcggcgca cagccgctgc
      481 caaccgttcg acagtgccag tgccaatggc gtgcgcccct cgtcaccggc gttgaacgct
      541 tcggctccac gttcgagtac gcgcagtgca aaagtttcca gatccggctg cggaataccc
      601 gcagccatcg cggcgatgca aatttgcgcg atcgcgtcgc caccggtggg ttgcgcgccg
      661 gcatccacga gatcgcttgc cgctgccaaa gtttccggca gctgcaacaa cgcgcgccgc
      721 agcaatggca agccgtcgag gctggcgttc gcatcgagtc cgtgattgag cagccagcga
      781 cgcggcgcgg catcggaatg atcgatcagt tcgccaaata gccgcgccac ggtctgcggc
      841 ggccagtcgc ggatctcacc ggcgaaattc tccgcaatct gccagtgccc gaagcgcagc
      901 gcgtcgagca ggtggtcggg cacatccttg cttaactcca cggccttgtc tgcctgcaga
      961 tgtgccggtc gcggatactc gggatcgatc gcggcaacga tgttccagcg tccggcggcg
     1021 gcggcgaaat cgaccgcgcg ccgtccgtcc ttggcggcaa gctgacgcga aatgccgaag
     1081 gcgagcaagc gccgcacggt ttcctcgccc gcgcacagcg actgcgcggc aacggtcagc
     1141 gcgctgcggc cggcgtgatc gaccgcatcc ggcggcggcc gcgacgctat caacaggtcg
     1201 agtacggcat gcgcgttggc acgcgccgct tccatcagcg ccgtcgtgcc gtgcgcgtcg
     1261 gccgcatcca cgcgtgcgcc ggcatcgagc agcacgcgcg cgatgactgc gtggccgcgc
     1321 agtgcggcgg ccatcaaggg ggtacgctgc agggcatcgt gcacatccac gcgcgccttg
     1381 cgcttgagca acaatttcac gccgtccgga tcgtcgtcgt cgatcgaagc cgctgcgatc
     1441 aacgccggct gcgcccggct ggtttcgggt tttgcgccac gctccagcag aaagcgtgcc
     1501 aattcccaat tccccgccgc acaggccatg acgagtggcg tttcgccggc acgattgatc
     1561 gcatcgagcc gagcgcctgc atcgcacagc agtgcggcaa cgacgggttt ggcggaaagc
     1621 gcggcgaaat gcagcggtgt gttgccatcg gcgtcgttgc aattcacggc tgcgccgttg
     1681 gtcaacaagg tcatcacggc ctcggcacgc ccttcgcggc tgtcgcgcgt cgccgcgatc
     1741 aacaacggca atccggcatg cgcgtgctgc aaattcgcgc ccttggcaat gaagccgcgc
     1801 agcagtcgtg tgtccggact cagcgccgcc aataccagtg cgctgcgctg gtcacggtca
     1861 tcggcaggtg gaacgccatt cggatccgcg ccatgctcca atgccgcgag cgcgagctgc
     1921 gtctgtcctg cgcgcacgca acgcaacagc atggcatcca gatccccggc at
//
LOCUS       DASRVO010000185         1689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_317146, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000185 DASRVO010000000
VERSION     DASRVO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317146"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VFF05_02205"
     CDS             <1..1278
                     /locus_tag="VFF05_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZW50650.1"
                     /translation="AQTYLRDPIEIAIKNKTTTAANIRQRYWYVSGMHKLDALTRILE
                     AETFEAMLVFMRTKQATEEIAQKLQARGFAAVAINGDVIQAQRERAIAQLKDGQIDIL
                     VATDVAARGLDVERISHVLNYDIPTDTEAYVHRIGRTGRAGRSGEAILFVAPREKRLL
                     QAIERATRQPITEMQLPSVDAVNDRRTTKFKQRITDTLASDDLAPFRQLVEQYERERN
                     VPAVEIAAALARLVQGKQPLLLQPAPRESRPFEKPRAEKPSQHEHLPNRAPARSHQST
                     PSPARRDERPRKAEAGFETYRIAVGNDHGVRPGNIVGAIANEAGLDSKHIGRIDIRDR
                     YTLLDLPTGMPEETFQLLKTVRVSGQRLLIARASEPPETAPTSGKPPRKPPFRPTAHK
                     SKPVKPGGFDKGGFGKGGVDKRRHGPHIREKAR"
     gene            complement(1275..>1689)
                     /locus_tag="VFF05_02210"
     CDS             complement(1275..>1689)
                     /locus_tag="VFF05_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinic acid mononucleotide
                     adenylyltransferase"
                     /protein_id="HZW50651.1"
                     /translation="LDRSEPSYTVDTLRQMRTEYGAARSLILLLGADAFAGLPTWHNW
                     RELLDLAHIAVMTRPGHDTSLPANLETVVVARRASGVADLRATPSGKVLDLPVTPLEV
                     SASAVRALLAAGRDPRWLVPDALLADRDLLAPYRN"
BASE COUNT          374 a          578 c          501 g          236 t
ORIGIN      
        1 gcgcaaacct atctgcgcga tccgatcgaa atcgcgatca agaacaaaac gaccacggcc
       61 gcgaatatcc ggcagcgtta ctggtatgtc agcggcatgc acaagctcga cgcgctcacg
      121 cgcattctcg aagccgaaac gttcgaggcg atgctggtat tcatgcgcac caagcaggcc
      181 accgaggaaa tcgcgcaaaa gctgcaagcg cgcggattcg ccgccgtcgc gatcaacggc
      241 gacgtcattc aggcgcaacg cgagcgcgcc atcgcgcaac tcaaggatgg tcagatcgac
      301 atcctggttg ccaccgacgt tgccgcgcgt ggactcgacg tcgagcgcat cagtcatgtg
      361 ctgaactacg atattcccac cgacaccgaa gcctacgtgc accgcatcgg tcgcaccggc
      421 cgcgccgggc gcagcggcga ggcgatcctg ttcgtggccc cgcgcgaaaa gcgcctgctg
      481 caggcgatcg agcgcgccac gcggcagccg atcaccgaaa tgcaattgcc cagcgtggat
      541 gcggtgaacg atcggcgcac tacgaagttc aagcaacgta tcaccgatac gctggcaagc
      601 gacgatctgg ctccgttccg acaactggtc gaacaatacg aacgcgaacg caacgtgccg
      661 gccgtggaaa tcgccgccgc gctcgcgcgt cttgtgcaag gcaagcagcc gttgttgctg
      721 caaccagcgc cgcgcgaatc gcggccattc gagaaaccgc gcgcggaaaa accatcgcag
      781 catgagcacc tgccaaatcg tgctcccgca cgttcgcatc agtcgacgcc ttcgcccgcg
      841 cgccgcgacg aacgcccgcg caaggccgaa gcgggattcg aaacctatcg cattgccgtc
      901 ggcaacgatc acggcgtccg ccccggcaac atcgtcggcg cgatcgccaa cgaggcaggc
      961 ctggacagca agcacatcgg tcgcatcgac attcgcgaca ggtacaccct gctcgatctt
     1021 cccaccggca tgcccgagga aacgttccag ttgctcaaga cggttcgggt aagcggacaa
     1081 cgattgctca tcgcccgcgc gagcgagccg ccggaaacag cacccacatc cggcaaaccg
     1141 ccgcgcaaac cgccgttcag gccgactgcg cacaaatcga aaccggtcaa gccaggcgga
     1201 ttcgacaagg gtgggttcgg caaaggtggg gtcgacaaac gcaggcatgg gccgcacata
     1261 cgcgagaaag cgcgctaatt tcgatacggc gccagcaaat cccgatcggc caacagcgcg
     1321 tccggaacca gccagcgcgg atcgcggccg gcggccagca acgcgcgcac ggcgctagcc
     1381 gaaacctcca gcggagtgac cggcaaatcc agtaccttcc cgctcggcgt tgcgcgcaaa
     1441 tcggccaccc ctgacgcgcg gcgtgcgacg acaaccgtct ccagattcgc gggcaggctc
     1501 gtgtcgtgac ccggccgcgt cattaccgcg atatgcgcga ggtccaacag ttcgcgccag
     1561 ttatgccagg tcggcaatcc ggcaaacgca tccgcgccga gcagcaggat caacgaacgc
     1621 gccgcaccgt attccgtgcg catctgccgc aaggtatcga ccgtgtacga cggttcgctg
     1681 cgatccagt
//
LOCUS       DASRVO010000186         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_57325, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000186 DASRVO010000000
VERSION     DASRVO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57325"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFF05_02215"
     CDS             complement(<1..604)
                     /locus_tag="VFF05_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HZW50652.1"
                     /translation="MLQSAFAVAAIVAAGVGLSGAGVATSGDAAYGKQIQDWRHERVE
                     HLRAPHGWLSLVGLDWLKDGKNTLGSAKGNDIVLAKAPAHLGTIVLDHGKATIALDAK
                     SGTTIDGKSLATAELLDDSHDKPTTVAFGTVSFYLVQRGDKYGLRVKDSEAPTRTHFL
                     GIDYFPIDASWRIEAKWEAYNPPHEVEEPNILGQVDKVVVP"
     gene            complement(631..>2188)
                     /gene="mutL"
                     /locus_tag="VFF05_02220"
     CDS             complement(631..>2188)
                     /gene="mutL"
                     /locus_tag="VFF05_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZW50653.1"
                     /translation="LALAQHATSKIATLEDLEHVRTLGFRGEALASIASVSRFALTSR
                     VAGADAAWRVAREGGKLDAARPAQQGPGTTIEVRDLFHNVPARRKFLRAERTEFGHID
                     ELVKAIALARPDVDIRLSHNTKPVRLLRPANDEAALAARVGDILGVEFPANSLRVDHA
                     AAGLRLSGWLGLPTASRSQPDQQFFYVNGRLVRDRVVTHAVRQAYADVLFHGRHPAYV
                     LFLELPPEGVDVNVHPAKSEVRFRESRLVHDFLYRTLNEALAQTRAGAESVPATVPHP
                     AMAYAPTVQSGLGLGVREPLADYAALFGTASARLATNETSIPESTSNEVPPLGFALAQ
                     LAGVYVLAENAQGLVMVDMHAAHERITYEKLKTARACDGIHSQPLLVPLSLAVSEREA
                     AVAEEHAENLAALGFEIARSGPQSVMVKRFPGILDGADVGGLVRDVLGDLATHGSSRR
                     LQETQNEILSTMACHGSIRANRRLTLPEMNALLREMEATERSGQCNHGRPTWVQLPMA
                     ELDRMFLRGR"
BASE COUNT          367 a          723 c          704 g          394 t
ORIGIN      
        1 ccggcaccac gaccttgtcg acctggccga ggatattcgg ctcttcgact tcatgcggcg
       61 gattgtaggc ctcccatttc gcttcgatgc gccagcttgc atcgatcggg aaataatcaa
      121 tgccgaggaa atgcgtgcgc gtcggcgctt cgctgtcctt gacgcgcagg ccgtatttgt
      181 cgccgcgctg gaccaggtag aagctgaccg tgccgaaggc gaccgtggtc ggcttgtcat
      241 ggctgtcgtc gagcaactcc gctgtcgcaa gcgatttgcc gtcgatcgtc gtaccggact
      301 tcgcatccag cgcgatcgtg gccttgccgt ggtccagcac gatcgtgccc agatgtgcag
      361 gcgccttcgc cagcacgatg tcgttgccct tggccgagcc gagcgtgttc ttgccatcct
      421 tgagccagtc caggcccacc agcgacagcc agccgtgcgg cgcgcgcaga tgctcgacgc
      481 gctcgtggcg ccaatcctgg atctgtttgc cgtatgccgc atcgccgctt gtggcaacgc
      541 cagcgccact caaacctact cccgcggcga cgatcgccgc gactgcgaat gccgattgca
      601 acatggtcca ctccagtacg tgaataggat tcagcgaccg cgcaggaaca tcctgtccaa
      661 ttccgccatc ggcagctgca cccaggtcgg acgcccgtgg ttgcactggc ccgaacgctc
      721 ggtcgcttcc atctcgcgca agagtgcgtt catttccggc aaagtcaagc gccgattggc
      781 acggatcgaa ccgtggcagg ccatcgtgga caggatttcg ttctgcgtct cctgcagccg
      841 gcgcgagctg ccgtgcgtgg ccaggtcgcc gagcacgtcg cgcaccaagc cgcccacatc
      901 ggcgccgtcg agaattccag gaaagcgttt aaccatcacg ctctgcggtc cgctgcgcgc
      961 gatttcgaaa ccgagcgcgg cgagattttc cgcgtgttct tccgccaccg cagcttcgcg
     1021 ttcgctgact gcaaggctca gcggcaccag cagcggctgc gaatgtatcc cgtcgcaggc
     1081 gcgcgccgtc ttgagctttt cgtaggtgat gcgctcgtgc gcggcgtgca tgtcgaccat
     1141 gaccaggccc tgcgcgtttt cggcaagaac atagacgccg gcgagctgcg cgagcgcaaa
     1201 gccgagcggc gggacttcgt tcgacgtgct ttcgggaatc gatgtttcat tcgtcgcgag
     1261 gcgtgccgat gccgtgccga acaatgccgc gtaatcggcc agcggttcgc gcacgccaag
     1321 accgagtccc gactgtaccg tcggcgcata cgccatggcg ggatgcggca cggtcgcagg
     1381 caccgattcc gcgcctgctc gcgtctgcgc caacgcctca ttgagtgtgc gatacaggaa
     1441 atcgtgcacg aggcggcttt cgcggaaacg tacctcggat ttggccgggt gcacgttcac
     1501 atcaacgcct tcgggcggca gttccaggaa cagcacgtaa gccggatgac gaccgtggaa
     1561 cagcacgtcg gcataggcct ggcgcaccgc atgggtgacg acgcgatcac gcacgaggcg
     1621 tccgttgacg taaaagaatt gctggtccgg ctggctgcgc gaagcggtcg gcagcccgag
     1681 ccagccggac aggcgcagcc cggcggccgc gtgatccacg cgcaagctgt tcgccggaaa
     1741 ttccacgccg agaatgtcac cgacgcgcgc cgcaagcgcg gcctcgtcgt tcgccggacg
     1801 cagcaggcgc acgggtttgg tgttgtgcga caggcggatg tcaacgtcgg gccgcgccag
     1861 cgcgatggcc ttcacgagct cgtcgatgtg gccgaattcg gtacgctcgg cgcgcaggaa
     1921 cttgcgccgc gccggcacgt tgtggaacag gtcacgcacc tcgatcgtcg tgccgggccc
     1981 ctgctgcgcc ggtcgcgccg catcgagctt gccgccttcg cgcgcgacgc gccaagctgc
     2041 gtccgctccc gcgacacgcg aagtcagcgc aaagcgcgaa actgaagcga tcgaagccag
     2101 cgcctcgccg cggaatccga gcgtgcgcac gtgttccagg tcttcgagcg tggcgatctt
     2161 gctggttgcg tgctgggcga gtgcgagc
//
LOCUS       DASRVO010000187         1480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_187702, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000187 DASRVO010000000
VERSION     DASRVO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187702"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFF05_02225"
     CDS             complement(<1..776)
                     /locus_tag="VFF05_02225"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HZW50654.1"
                     /translation="MSRGITDGIILAHRYGFLTSASLMANMPAAEYAVARARAFPRLA
                     VGLHLNICQGRPLLPASEVSSLVGADGNFHPPREMARRLWTQRAAPAEIEREFREQIL
                     WMKGRGLPPSHADSHHHMHLYPAAARAFARALAAEAIPCARSCRCTEWPKGRAIGGPY
                     GGGVVRRVCVQAYRRALQRTILRRFGSPDSRVAISLGSAPRSVDLRDRWKTTLENLPP
                     GTFELSCHPGLFERGFSEGDAIRMRREEELLCLADHDVRGV"
     gene            complement(815..>1480)
                     /locus_tag="VFF05_02230"
     CDS             complement(815..>1480)
                     /locus_tag="VFF05_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50655.1"
                     /translation="GIPMRAVFTSGEPLYASIREKTEQAFQAPVFDSYGMTELCGLIQ
                     QCERGRMHLIPDYGFLEILGEDDRPALGGAEGHFVWTGFINSAMPLIRYRIGDSGRWL
                     AGETCPCGREFPLVLPTITRESDLLRCPDGRVFSPRALNQLLKNSNSLRSCQFIHERR
                     ERVLVRAVAGNGRALEEMMRIRAGLQELLGPHMEVTADLAREPFTGPGGKIPLIVNRA
                     GPQ"
BASE COUNT          276 a          488 c          486 g          230 t
ORIGIN      
        1 acgccgcgca cgtcgtggtc cgcgaggcac agcaactctt cctcgcgccg catgcgaatg
       61 gcgtcgcctt ccgagaagcc gcgctcgaac agcccgggat ggcaggagag ttcgaacgtt
      121 cccggcggca gattttcgag cgtcgtcttc cagcgatcgc gcagatcgac ggaacgcggc
      181 gcggatccca acgaaatggc aacgcggctg tcgggtgaac cgaaccgccg gagaatcgtg
      241 cgctgcaagg cgcggcggta ggcctgcacg caaacccggc gaacgacgcc gccgccatag
      301 ggaccgccga tcgcgcggcc cttgggccac tccgtgcaac gacaggatcg cgcgcaggga
      361 atcgcttcgg cggccagcgc ccgcgcaaac gcccgggccg ccgccggata gaggtgcatg
      421 tgatgatgcg agtcggcatg cgacggtggc aggccgcgac ctttcatcca gaggatctgc
      481 tcgcgaaatt cacgctcgat ctccgccggc gcagcacgct gcgtccacaa gcggcgcgcc
      541 atttcgcgcg gcggatgaaa attcccgtcc gcaccgacca ggctggacac ttcgctcgcg
      601 gggagcagcg gccgtccctg gcagatgttg agatgcaggc cgaccgccag gcgcggaaac
      661 gcccgcgcgc gcgccaccgc atactctgct gcgggcatgt tggccatcag agacgcgctg
      721 gtcagaaaac catagcgatg cgcgaggatg atgccgtcgg tgatcccgcg gctcatccca
      781 aaatcatccg cgttgacgat taaacggatc gctttcattg cggccccgca cgattcacga
      841 tcagcggtat ctttcctccc ggcccggtga acggttcgcg cgccaagtcg gcggtcactt
      901 ccatgtgggg gccgagcagt tcctgcaagc cggcgcgaat ccgcatcatt tcttcgaggg
      961 cgcggccgtt gccggccacg gctcgcacca acacgcgctc gcggcgctcg tggatgaatt
     1021 ggcaggagcg cagcgaattc gaatttttca gcagctggtt caaagcgcgc ggggaaaata
     1081 cgcggccatc ggggcagcgc agcaggtcgc tctcgcgcgt gatggtagga agcaccagcg
     1141 ggaattcccg gccgcagggg catgtctcgc ctgccagcca gcggccagag tctccgatgc
     1201 ggtagcggat cagaggcatc gccgaattga tgaaacctgt ccacacgaag tggccctcgg
     1261 cgccgcccag cgccggccgg tcgtcctcgc ccagaatttc gaggaagccg taatcgggga
     1321 tcaggtgcat gcgcccgcgc tcgcactgct ggatcaagcc gcatagctcg gtcatgccgt
     1381 aggaatcgaa aacaggcgcc tgaaaggcct gctcggtttt ctcgcgaatc gaggcgtaca
     1441 acggctcccc gctggtgaac acggcgcgca tggggattcc
//
LOCUS       DASRVO010000188         1199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_238780, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000188 DASRVO010000000
VERSION     DASRVO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238780"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFF05_02235"
     CDS             <1..772
                     /locus_tag="VFF05_02235"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZW50656.1"
                     /translation="QGGTAVGNVDGGGAGMGGAIFNHRGALSLINVTMTGNTAIGGSG
                     SNTYPGSGLGGAIFNLNGTVTIQFSTLANNSVSGSNGEGLISGGDGTVYSLAYGSKIE
                     DANASNAALTIDDSIVSGTTASSGAGGHDVVNNAVAWSLTANVATLNFTGSNIVGTSQ
                     NGPDSAGATATQNGAPTSSAAPMLGALFNYGGPTQTMRPNSGSPAIGAANCTNAPPTD
                     QRGVTRPQNGVCDLGSVELTVNERDRIFADNFDGTPTL"
     gene            complement(814..>1199)
                     /locus_tag="VFF05_02240"
     CDS             complement(814..>1199)
                     /locus_tag="VFF05_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HZW50657.1"
                     /translation="QIKLVHITCVVLSGSLFTLRGVMMLAGSVRVYHPALKYLSYLID
                     TTLLTSAVLLTLILHQYPFVQSWLTAKVLLLVVYIVLGVFALRRGRSRVSRAAYFVAA
                     LAVFAFIVSIAIAHDPRGIFARFAG"
BASE COUNT          253 a          394 c          391 g          161 t
ORIGIN      
        1 ccagggtggc acagccgttg gcaatgtcga tggcggcggc gcgggcatgg gtggcgcgat
       61 cttcaatcat cgcggcgcgc tgagcctgat caacgtgacg atgaccggca acaccgcaat
      121 cggcggcagc ggctccaata cctatcccgg cagcggtctc ggcggcgcga tcttcaatct
      181 caacggcacc gtcacgatcc agttttccac gctggcgaac aattccgtca gcggcagcaa
      241 cggcgagggc ctcatcagcg gcggcgacgg cacggtgtat tcgctcgcct atggcagcaa
      301 gatcgaggac gcaaacgcga gcaacgctgc gcttaccatc gacgacagca tcgtctcagg
      361 taccacggcc agcagcggcg ccggcggcca cgatgtcgtg aacaatgccg tggcctggag
      421 cctcacggcc aacgtcgcca cgctgaactt cactggcagc aacatcgtcg gcaccagcca
      481 gaacggcccg gacagcgccg gcgccaccgc gacgcagaat ggcgcgccga ccagcagcgc
      541 cgcgccgatg ctcggcgcgc tgttcaatta cggcggcccg acgcagacga tgcgaccgaa
      601 ttcaggcagc ccggccatcg gcgcggcaaa ctgcacgaat gcaccgccta ccgaccaacg
      661 cggcgtgaca cggccgcaaa acggagtctg cgatctcggc tcggtggagc tgaccgttaa
      721 cgaacgcgat cgcatcttcg ccgacaactt tgacggcacg ccgacgctgt agcggcaaag
      781 cgctctagcc ccggtcgatc ggcatgtgcg cgttcaaccg gcgaagcgcg cgaagattcc
      841 ccgcggatcg tgtgcaatcg cgatgctgac gatgaaggcg aacaccgcga gcgcggcgac
      901 gaagtacgcc gcgcggctca cgcgcgagcg cccgcgccgc agcgcgaaca cgccgagcac
      961 gatgtagacc acgagcagca gcaccttggc ggtgagccag gattgcacga acggatactg
     1021 gtgcaggatc agcgtcagca gcaccgccga cgtgagcagg gtcgtgtcga tgaggtagga
     1081 aagatacttc agcgccggat ggtagacgcg cacggaaccg gccagcatca tcacgccgcg
     1141 cagcgtgaac agactgccgg agaggacgac gcaggttatg tggacgagtt tgatctgcg
//
LOCUS       DASRVO010000189         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_317378, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000189 DASRVO010000000
VERSION     DASRVO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317378"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..>1071)
                     /gene="cfa"
                     /locus_tag="VFF05_02245"
     CDS             complement(109..>1071)
                     /gene="cfa"
                     /locus_tag="VFF05_02245"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HZW50658.1"
                     /translation="MDGWWDAADLDGLLFRLLSAHVDERVHGIDDVWLWLKSSLINLQ
                     RGRRAFTIGERHYDLGNDLFEAMLGKRLVYSCGYWNAAANLDAAQEAKLDLVCRKLML
                     QPGMRVLDIGCGWGEALKFAAEHYGVSGVGITVSHEQAEYAKNLCRGLPIEIRVQDYR
                     AVDERFDRVYSIGMFEHVGVKNYSTYFEVNRHCLADDGLALLHCIGSNVSSNRTDPWI
                     AKYIFPNSMLPSVEQIASAREGVFVIEDWHNFGADYDTTLMAWRSNFDAAWPRLSQRY
                     DERFRRMWRFYLSASAATFRARRDQLWQLVLSPRGVPGGYRAPR"
BASE COUNT          199 a          331 c          351 g          190 t
ORIGIN      
        1 acggtgcagt tttcccgttt gctgccgtca gcatagcgca agcgtgccgg atttcgccgt
       61 cgcatgccga cgcacgcgga ggctttggtt tggcggtgcg gcaattccct agcgcggcgc
      121 gcgatacccg ccgggtacac cgcgcggact gagcacgagc tgccagagct ggtcgcggcg
      181 cgcgcggaaa gtggcggccg aggcggagag gtagaagcgc cacatgcgcc ggaagcgttc
      241 gtcgtagcgt tgcgacaggc gcggccaagc ggcgtcgaaa ttcgagcgcc aggccatcag
      301 ggtcgtgtcg tagtcggcgc cgaagttgtg ccagtcctcg atcacgaaca cgccctcgcg
      361 cgcgctggcg atttgctcga ccgacggcag catggaattg ggaaaaatat acttcgcgat
      421 ccacgggtcc gtgcggttcg aggacacatt gctgccgatg cagtgcaaaa gggcgaggcc
      481 gtcgtcggcg agacaatggc gattgacctc gaaataggtg ctgtaatttt tcacgccgac
      541 gtgttcgaac atgccgatcg aatacacgcg gtcgaagcgc tcgtccacgg cgcgataatc
      601 ctgcacgcgg atttcgatcg gcaatccgcg gcacaggttt ttcgcgtact cggcctgttc
      661 gtgcgaaacg gtgatgccaa cgcccgatac gccgtagtgc tcggcggcga acttcaacgc
      721 ctcgccccaa ccgcagccga tgtcgagaac gcgcatgccg ggttgcagca tcagcttgcg
      781 gcagaccagg tcgagcttgg cttcctgcgc cgcgtcgaga ttcgcggcgg cgttccagta
      841 gccgcaggaa tacaccaagc gcttgccgag catcgcctcg aacaggtcgt tgccgaggtc
      901 gtaatggcgt tcgccgatcg tgaacgcgcg ccgtccgcgc tgcagattga tcaggctgga
      961 tttgagccac agccagacat cgtcgatgcc gtgcacgcgc tcgtccacat gcgcgctgag
     1021 caggcggaac agtaagccat cgaggtcggc cgcgtcccac cagccgtcca t
//
LOCUS       DASRVO010000190         1191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_31793, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000190 DASRVO010000000
VERSION     DASRVO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31793"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /gene="dut"
                     /locus_tag="VFF05_02250"
     CDS             complement(<1..442)
                     /gene="dut"
                     /locus_tag="VFF05_02250"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZW50659.1"
                     /translation="MIAVELKILDPRVGMEIPLPAPATAGSAGMDLRAALDAPLTLVP
                     GESALIPTGIAIHVGDPDYCAVILPRSGLGHKHGIVLGNLTGLIDADYQGPLMISCWN
                     RAQAPYTIAPGERIAQLVFLPVARVQFRVVEEFAASQRGAGGFGH"
     gene            complement(439..>1191)
                     /gene="coaBC"
                     /locus_tag="VFF05_02255"
     CDS             complement(439..>1191)
                     /gene="coaBC"
                     /locus_tag="VFF05_02255"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZW50660.1"
                     /translation="PESGPGRLLEPHQIVAELASLHGPKPLAGLRVLVSAGPTCEDID
                     PVRFIGNRSSGRMGFAVAQAAAEQGAAVHLVAGPVHLPTPPDVSREDVRSARQMRDAV
                     MRQIPQCDIFISAAAVGDYRPADVAAHKLKKTGKSLVLELTENPDIVAEVAALKKRPF
                     VAGFAAETENLERHARAKLERKGLDLVAANRVGGGAGFDADENELTLLWRGGKQGLPR
                     ASKRDLAHALLTRIEALRAQKNRTNKPGARRR"
BASE COUNT          203 a          397 c          389 g          202 t
ORIGIN      
        1 tatggccaaa cccgcccgcc ccgcgctggc tggcggcgaa ttcttccacc acacgaaact
       61 gcacccgcgc caccggcagg aataccagtt gcgcgatgcg ctcacccggc gcaatggtat
      121 agggcgcctg cgcgcggttc cagcacgaaa tcatcagcgg gccctggtaa tcggcatcga
      181 tcaagccggt gaggttgccg agcacgatgc cgtgtttgtg gccgaggccc gagcgcggca
      241 ggatcaccgc acaatagtcc ggatcgccga catggatggc gatgccggtg ggaatcagcg
      301 cgctttcgcc gggcacgagg gtcagcggcg catccagtgc cgcgcgcaag tccatgcctg
      361 ccgatccagc cgtggcgggt gcgggcaagg gaatctccat gccgacgcgc ggatcgagta
      421 tcttcaattc cacggctatc atcgacgccg cgccccgggt ttgttcgtcc tgtttttttg
      481 cgcgcgcagc gcctcaatac gcgtgagcag cgcatgcgcc agatcacgct tgctcgcacg
      541 cggcaaccct tgtttgccgc cgcgccaaag cagggtcagc tcgttctcgt cggcatcgaa
      601 cccggcaccg ccgccgacgc gattggcggc gaccaagtcc agccccttgc gctcgagctt
      661 ggcgcgggcg tggcgttcga ggttctcggt ctccgccgcg aatccggcga cgaaaggtcg
      721 ttttttcaac gccgcgactt cggcaacgat atccggattc tcggtgagtt cgagcacgag
      781 cgacttgccg gttttcttga gtttgtgcgc agccacgtcc gcggggcgat agtcgccgac
      841 agccgcggcg gaaataaaaa tgtcgcattg tggaatttgc cgcatcacgg cgtcgcgcat
      901 ctggcgcgcg ctgcgcacgt cctcacgcga cacgtccggc ggcgtgggca gatgcacggg
      961 accggcaacc agatgcacgg ccgcgccctg ctccgccgca gcctgggcga cagcgaaacc
     1021 catgcggccg gaacttcgat tgccgatgaa acgcaccgga tcgatgtctt cgcaggttgg
     1081 accggcgctg accagcacgc gcaggccggc cagcggtttc gggccatgca gcgacgcgag
     1141 ttccgcgacg atctggtgcg gctcaagcaa gcggccggga ccggattccg g
//
LOCUS       DASRVO010000191         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_343091, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000191 DASRVO010000000
VERSION     DASRVO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343091"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VFF05_02260"
     CDS             complement(<1..1342)
                     /locus_tag="VFF05_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HZW50661.1"
                     /translation="MLALGVVAVSAVALPPNQATPARTRSAGAMAAGGRSQTNSTAEA
                     AAAAKLPIRRVILYKTGVGYFEHLGEVTGDQTVAIDFTSGQLNDVLQSLTVLDLGGGR
                     ITGVDYNSQAPFSQRLGMLRLPLTEQTDVAKFYGALRGARLEVRSGASSITGRLLSVQ
                     QDTRVVDRTAVQVNILTLVSDAGDVRSIDLTPGVTVRLADRDVNEQVGRYLSLLSSVR
                     QQDLRRMSISTSGAGARQLFVSYISEVPVWKTTYRIVLPSKAGDEPLLQGWAIVDNTV
                     GEDWDNVELSLVAGAPQSFIEQLSQPYYTRRPVVPLPQMAQLTPQTHESAMLGGLGAV
                     SGTVTDPSGATITGAQVKLFDSSGNLIGSQVSDDQGRYEFDDLPSATYRLEISQPGFQ
                     RSVVSGLSVSGDNQLTQNVTLRLGAVSESVEVNAAAPAPAPAPMKLYARNQLGGV"
     gene            1631..>3026
                     /gene="purL"
                     /locus_tag="VFF05_02265"
     CDS             1631..>3026
                     /gene="purL"
                     /locus_tag="VFF05_02265"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HZW50662.1"
                     /translation="MATIDTEIKVTPQIAAEHGLTPDEYARIRKILGRDPNITELGVF
                     SVMWSEHCSYKSSRVHLKRLPTRGARVVQGPGENAGVVDVGDGLVAVFKIESHNHPSF
                     VEPFQGAATGVGGILRDIFTMGARPIAVLDSLRFGPIAPGPAAGSAAAEEIHRNRQIL
                     DGVVRGIAFYGNCFGVPTVGGEVAFEPCYSNNPLVNALALGVGRRDELFFARASGAGN
                     PVIYVGAKTGRDGIHGASLLASAEFSEESQQKRPNVQVGDPFMEKLLLEACLEAMRTG
                     AVVAIQDMGAAGLTSSSSEMASRGGVGIEIDLSRVPQRETGMTPYEMLLSESQERMLL
                     VAERGREHEVFAVFKKWGLDAVEIGRVTNDGMLRVLHERRVAAAIPAHALAEEGPRYE
                     RPIAKPARAAGEPEAPLVEFAREGADLSGNFRRLLASHTIASKYWITEQYDSMVRTNT
                     RVGPGAGDAAVLRLK"
BASE COUNT          537 a          916 c         1042 g          531 t
ORIGIN      
        1 caactccgcc gagctggttg cgcgcgtata gcttcatcgg agcgggagcc ggcgcggggg
       61 cggcggcatt gacttcaacc gactcgctga cggccccgag ccgcaacgtc acattctgag
      121 tgagttgatt gtccccactg actgagaggc cgctgacaac ggatcgctgg aatcccggct
      181 gactgatttc cagacggtaa gtggcggagg ggagatcgtc gaattcatat cgcccctggt
      241 cgtcactgac ctgtgagccg atcagattgc ctgagctgtc gaagagcttg acttgcgcgc
      301 cagtgatcgt cgccccagag ggatcggtga ccgttccgga gaccgcgccc agcccgccaa
      361 gcatggcgct ttcgtgggtt tgcggtgtga gttgcgccat ctgcggaaga ggcacgaccg
      421 ggcggcgcgt gtagtagggc tgggagagct gctcgatgaa ggattgcggc gcgccggcca
      481 ccagcgacag ctcgacattg tcccaatctt cgcccacggt gttgtcgacg atggcccagc
      541 cttgcagaag gggttcgtcg cccgccttgg aaggcagcac gatgcgatag gtcgtcttcc
      601 aaacgggcac ttcgctgatg tagctgacga acagctggcg cgcgcccgcg ccggaagtcg
      661 agatgctcat gcggcgcaaa tcctgctggc ggacggagga aagcaggctg aggtaacggc
      721 cgacttgctc gttgacgtcg cgatcggcaa gccgcacggt cactccgggc gtcagatcga
      781 tgctgcgcac gtcgcccgcg tcgctgacca gcgtcaggat gttcacttgc accgccgtgc
      841 gatccaccac gcgcgtatcc tgctgcacgc tgagcaagcg gccggtgatg ctgcttgcgc
      901 cgctgcgcac ctcaagccgc gcgccgcgca gggcgccgta aaacttcgcg acgtcggtct
      961 gctcggtgag cggcagccgc agcatgccga ggcgctggct gaagggcgcc tgcgagttgt
     1021 aatccacccc ggtgatgcgc ccgccgccaa ggtcgaggac ggtgagcgac tgcagcacgt
     1081 cgttgagctg accgctggtg aaatcgatgg cgacggtctg atcgccggtg acttcgccga
     1141 gatgctcgaa atagccgact cccgtcttgt agaggatgac gcggcggatg gggagcttcg
     1201 ccgccgcggc cgcctcggct gtcgaattcg tctgggagcg cccgcccgct gccattgcgc
     1261 cggctgatct agttctcgcc ggagtcgctt gattcggcgg cagcgccacc gccgagaccg
     1321 ccaccacgcc taaggccagc acgcacgcgc acacaacggc tgttcgcata agccctcctg
     1381 ttttgcggat gtcccgactg cgtattatgc cgtgatgatg cgcggtttat gagagcgtgt
     1441 acctatgcgt taaggggacg aacgcgccat tcgcctcctc gatgcgtcgc gccgtgctgc
     1501 gcacggatga ttagagaccg gcggcggtcg ttttgttgcc ggagcactcc ggcttttctg
     1561 cacgccggct taccgggcgc ttgcgtggga atggaattgc tgcgagaatt tgcgctccag
     1621 ccgccgacaa atggcaacca tcgacaccga aatcaaagtc accccgcaga tagccgccga
     1681 gcacggattg accccggacg agtacgcgcg catccggaag attctggggc gcgatccgaa
     1741 catcacggaa ctgggcgtct tcagcgtgat gtggagcgag cactgctcct acaaatcgtc
     1801 gcgcgtgcat ttgaagcgct tgccgacgcg cggtgcacga gtggtgcagg gccccgggga
     1861 aaatgcgggt gttgtggatg tgggcgatgg cctggtcgcg gtcttcaaga tcgaatcgca
     1921 caatcatccg agcttcgtcg agccctttca gggtgcggca accggcgtgg gcggaattct
     1981 gcgcgacatt ttcaccatgg gcgcgcggcc catcgccgtg ctcgattccc tgcggttcgg
     2041 tccgatcgcg ccgggcccag ccgccggcag tgccgcagcg gaagaaatcc atcgcaaccg
     2101 gcagattctc gacggcgtgg tgcgcgggat cgcgttctac ggcaattgtt ttggcgtgcc
     2161 cacggtggga ggagaagtcg cgttcgagcc gtgctactcg aataatccgc tggtgaacgc
     2221 gctggcgctg ggggtggggc ggcgtgacga gttgttcttt gcgcgcgcga gcggcgcagg
     2281 caatccggtg atctacgtcg gagcaaaaac agggcgcgat ggaattcatg gggcatcgct
     2341 gctggcctcc gcggaattca gcgaagaatc gcaacagaaa cggccgaacg tgcaggtggg
     2401 cgatccgttc atggaaaagc tgctgctgga agcctgcctg gaggcgatgc gcacgggcgc
     2461 ggtggtcgcg attcaggaca tgggcgcggc gggactgacc agctcgagtt cggaaatggc
     2521 ctcgcgcggc ggcgtgggca tcgagatcga cctgtcgcgc gtgccccagc gcgaaacggg
     2581 catgacaccg tacgaaatgc tgctcagcga atcgcaggaa cgcatgctgc tggtcgccga
     2641 gcgcggccgc gaacacgaag tcttcgctgt attcaagaaa tgggggctgg acgcggttga
     2701 gatcgggcgc gtgacgaatg acggcatgct gcgcgttctg cacgaacggc gcgtcgcggc
     2761 ggcaatcccg gcgcacgctc tggctgaaga gggcccgcga tacgaacgtc ccatcgcgaa
     2821 gccggcgcga gccgccggag aaccggaagc gccgctcgtc gaatttgcgc gcgaaggcgc
     2881 ggacctcagc gggaatttcc ggcggctgct cgcttcgcac accatcgcgt cgaaatactg
     2941 gatcaccgag cagtacgatt cgatggtgcg cacgaacacg cgcgtaggcc ccggagcggg
     3001 cgacgccgcg gtgctgcggc tgaagg
//
LOCUS       DASRVO010000192         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_57498, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000192 DASRVO010000000
VERSION     DASRVO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57498"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFF05_02270"
     CDS             complement(<1..576)
                     /locus_tag="VFF05_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50663.1"
                     /translation="MPRCLFLVFALLAAPAFAQPLAPDAGTAPLSPEMPAAQVLAQFH
                     HDEINLLALRGDAHSLLAAALMAQGDADDPARPKVLQTPALLARAQKADDADALVWWV
                     SAALECHGKGKPCPSDETLAQLEKSDPANAAVWMWALRPAQKPGSAATTRAALTSAAG
                     AARYDDYFGTLTSILYESAGILPIQENVLRAS"
     gene            complement(635..>1663)
                     /locus_tag="VFF05_02275"
     CDS             complement(635..>1663)
                     /locus_tag="VFF05_02275"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZW50664.1"
                     /translation="GRDAFYKGKIAHTIADYMKAQGGFLSYDDLASHHGEWVEPVSTN
                     YRGVDVWELPPNGQGIAALQMLNILEGYDFSKIPFDSAEHVHLFVEAKKLAYEDRAHF
                     YADPDFYKVPLDWLISKDYAAQRRKLVSPDRAMRSVDPGQQPKLHGDTIYLTTADRWG
                     NMVSLIQSNYRGMGSGMTPPGLGFILQDRGELFVLKDGYANTYAPGKRPFHTIIPSFI
                     TRDGKPWISFGVMGGDMQPQGHVQIVQNLIDWHMNLQEAGDAPRIQHEGSTSPEGQET
                     EMKDGGWVDLESGFSYSTVRGLMQMGHDVRFANGPYGGYQAIEWDAKNRVYIGASEGR
                     KDGQAAGY"
BASE COUNT          297 a          522 c          527 g          317 t
ORIGIN      
        1 gctcgcacgc aggacgtttt cctgaatcgg caggattccc gcggattcgt acaggatcga
       61 agtcagcgtg ccgaaataat cgtcgtaacg cgcggcacca gcagcgctgg tcagcgccgc
      121 gcgggtagtg gcggcactgc cgggtttttg cgcgggtcgc agcgcccaca tccagactgc
      181 ggcattggcc ggatcggact tttccagttg tgccaaggtc tcgtcgctcg gacagggttt
      241 ccccttgccg tggcattcca gcgccgccga aacccaccac accagcgcgt cggcatcgtc
      301 ggccttctgc gcgcgggcga gcagggccgg agtttgcagc accttcggtc gcgcgggatc
      361 gtcggcatcg ccctgtgcca tcagcgccgc ggcgagcagc gaatgcgcat cgccgcgcag
      421 ggcgagcagg ttgatctcgt cgtgatggaa ctgcgccagc acctgtgccg ccggcatttc
      481 cgggctgagc ggcgcggtgc cggcatccgg cgcgagaggt tgcgcgaagg cgggcgcggc
      541 gagcagggcg aagacgagaa acaggcagcg cggcatggcg gcgaatcctt gcgaaacgag
      601 acgtgcagtg tagcgaacgc cgataagtgc gagcttaata gccagccgcc tgcccatcct
      661 tgcgaccctc ggatgcgccg atgtagacgc gattcttggc atcccattcg atcgcctgat
      721 agccgccgta ggggccattg gcgaagcgca cgtcgtggcc catctgcatc aacccgcgca
      781 cggtcgagta cgagaatccg gattccaggt ccacccagcc gccgtctttc atttccgttt
      841 cctggccctc gggcgaggtc gagccttcgt gctggatgcg cggggcatcg ccggcttcct
      901 gcaggttcat gtgccagtcg atcaggtttt gcacgatttg cacgtgaccc tgcggctgca
      961 tgtcgccgcc catcacgcca aagctgatcc acggcttgcc gtcccgggtg atgaaactcg
     1021 ggatgatggt atggaacggg cgcttgccgg gcgcataggt attcgcgtag ccgtccttga
     1081 gcacgaacaa ttcgccgcga tcctgcagga tgaaaccgag tcccggcggc gtcatgcccg
     1141 aacccatgcc gcgatagttc gactggatca gcgacaccat gttgccccaa cgatcggcgg
     1201 tggtgagata aatcgtgtcg ccgtgcagtt tcggttgctg gcccggatcg accgagcgca
     1261 tcgcgcgatc cggcgaaacc agcttgcgcc gctgcgcggc gtaatccttc gagatcagcc
     1321 agtccagcgg taccttgtag aaatccggat ctgcgtagaa atgcgcgcga tcctcgtaag
     1381 ccagcttctt cgcttcgaca aacaagtgca catgttcggc gctgtcgaac gggattttcg
     1441 aaaaatcata accttccagg atgttgagca tctgcaacgc ggcgatgccc tggccgttcg
     1501 gtggcagctc ccacacgtcg acgccgcgat agttcgtcga taccggctcg acccattcgc
     1561 cgtgatgact ggcgagatcg tcgtaggaca agaatccacc ctgcgccttc atgtaatcgg
     1621 cgatggtgtg cgcgatcttg cccttgtaga acgcatcgcg gcc
//
LOCUS       DASRVO010000193         7015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_317514, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000193 DASRVO010000000
VERSION     DASRVO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317514"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..826
                     /gene="zipA"
                     /locus_tag="VFF05_02280"
     CDS             59..826
                     /gene="zipA"
                     /locus_tag="VFF05_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005991236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HZW50665.1"
                     /translation="MSWNASVGIPLAILGAIVFASIYWLGRPKKPRQGRRMVPPRESG
                     DRIEPTFGDDPAADDEAVFDAQQGELEVGMRGELQRLGSAIAAKRGEDGKGVVAKPAA
                     GTRPEQPIERIVTLFVAAPAEADFHGPDLVVAAEKAGLDFGDLGIFHRLVSGKPEAGP
                     VFSMANMVKPGSFDMTQIESLRTPGVSFFMTLPGPMSALDAWEAMLPTAQRLAELLDG
                     RVLDEGRNALGRQRIAHLRDELRAWDRKQERNQIRPR"
     gene            841..1458
                     /locus_tag="VFF05_02285"
     CDS             841..1458
                     /locus_tag="VFF05_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016657123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HZW50666.1"
                     /translation="MISASEALERLREGNRRFVADDCNGGQQAGKNRRRELAEGQEPF
                     ATILGCSDSRVPAEIIFDQGLGDLFVIRVAGNIIAPSLVGSVEFAAARFGTPLVVVLG
                     HTQCGAIAATIEELTQQGGAQSPNLQSIVNRVRPSVLPLMNTELRHDLHALSREAVRA
                     NIRASTNHLRHGSELMENLIEERGFRVVGAEYSLETGLVDFFDLG"
     gene            1550..1933
                     /locus_tag="VFF05_02290"
     CDS             1550..1933
                     /locus_tag="VFF05_02290"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HZW50667.1"
                     /translation="MANKRNKNQTTSPAGKAHAVWLASLGAVSIVQKRGTEVLSGLAA
                     EGRDFQARAQQLVREVGADAKVHVKGVVAPLRVDLKRNAAKFGVVVQNAVAGVLAKLG
                     IPSKADIEALSQRMSALSRQLKAAK"
     gene            complement(1984..2619)
                     /locus_tag="VFF05_02295"
     CDS             complement(1984..2619)
                     /locus_tag="VFF05_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZW50668.1"
                     /translation="MAAKQTRERILDASLAMFNAQGEPNVTTNHIADELGISPGNLYY
                     HYRNKDDIVERLFARYEARMDDALVAPEGRLPNLEDVWLQLHLVFECMWEYRFLYRDL
                     VDILARNRKLRQRFARILNRAAASAEAVLNGLAKAEILRATQDEIHATAENVLLVATF
                     WLNYHAVRGANPEPAQNDLSQGIYQVMLLIAPFLRDAERAHLNTLAQAYRR"
     gene            complement(2711..2968)
                     /locus_tag="VFF05_02300"
     CDS             complement(2711..2968)
                     /locus_tag="VFF05_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HZW50669.1"
                     /translation="MADLKTKFTQAAEDVKKLDERPDNDTLLKLYALFKQGSDGDVSG
                     PKPGFFDFVGTAKYEAWAKLKGMKADEAMQKYVDLVKKLRG"
     gene            3087..5075
                     /locus_tag="VFF05_02305"
     CDS             3087..5075
                     /locus_tag="VFF05_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003473835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZW50670.1"
                     /translation="MTYFVTGATGFIGRHLLELLLARKGDVHCLVRKGSQRKFEEVRE
                     RFGAEGKRLIPVVGDLTKPMLGLTPAQRKALAGKVKHFFHLAAIYDLAANADSQIAAN
                     VDGTRHALELADALKAGCFHYASSIAVAGFFPGVFREDMFAEAENLDHPYFRTKHDAE
                     ALVRAATIKHRIYRPAIVLGSSKTGEIDKIDGPYYFFRALKKLREALPAWMPTIGLEG
                     GRVNMVPVDFVVAAMDHIAHKPGLDGGCFHLTDPAPRRIGEVLNIFARAGHAPQMTMR
                     LDARMFGFIPPMIKQALAGLPPVKRFTGAVLRDLGIPAAAMRMINYPTRFDCRETERA
                     LKGTKIRVPDLQGYAWRLWDYWERHLDPDLFVDHSLSGKVRGRTVLITGGSSGIGKAA
                     ALKIAGAGAKVLICARGEDELNATRKEIVDAGGVCHTYVADLADQKSCDELVQKVLAE
                     HKGVDILINNAGRSIRRAIASSYDRFHDFERTMQLNYFGSLRLIMGFLPGMAARHRGH
                     IINISSIGVLTNAPRFSAYVSSKAALDAFSRCAQAEFSDNGIHFTTINMPLVKTPMIA
                     PTKMYESVPTLSPDEAADMIVDAIIQRPVRMATRLGTFAQIFYALAPRAYEILMNTAF
                     RLFPDSAAAKGMKGRGELDKQPTSEQVAFASLMRGVHW"
     gene            complement(5231..>7015)
                     /locus_tag="VFF05_02310"
     CDS             complement(5231..>7015)
                     /locus_tag="VFF05_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50671.1"
                     /translation="DPDDPQTVFGGRVDKLDLRTQQVRSVDPTLALPGLNRSVWTLPL
                     VFSPRQPHVLYFANQRVFRTDDHGEHWQAISPDLTRENPGTPKNLDPVTAADNLGQGP
                     RRGVVYALAPSPVADGLIWAGTDDGLIWRTRDGGTHWDNVTPNQLTPWSKVGIIDASA
                     FDADSAYAAVDRHRLDDQRPYIYRTRDGGKSWQLVVAGIPSNQSVNVVRADPKRRGLL
                     YAGTERGVYASFDDGEHWQTLQKNLPVTSVRDIAIHGADLVLATHGRGIWIMDDASSL
                     RQFDAKVEKAAFWLFKPEPAYRVRLPAFTGTPMPKDEPMAENPPLGAYLDYTLKIAIK
                     SPITLDIIDSTGALVRHYSSADPVAAVDPTKSPIAPEWADVPSTLSAATGTHRFVWPL
                     QYPAPPSGEKSDPWADGLFAPPGDYRVVLTVDGVKQEQPLQVVADPRVNLPASAYARQ
                     FALAKSIQAQNTRVSVAAREAGALIQALKKARVPDKELDREIGGLLERTAAITGAREA
                     PNPRNAWAYPPRDTRNFKFLADALAKLQAAVAGADAAPSVDAQNGFKALLPMVDAELN
                     SWDELKSHDLVALNAKLKAAGKEPIKIE"
BASE COUNT         1237 a         2099 c         2318 g         1361 t
ORIGIN      
        1 tggatctggc cgaagcggct aaactggcgg gtgccgccta gccgccggga gaacgcgaat
       61 gagctggaac gcatcggtcg gaattccgct cgccatcctc ggcgcgatcg tgttcgcgtc
      121 gatctactgg ctcggacgcc cgaaaaaacc gcggcaggga aggcgcatgg tcccgccacg
      181 cgagtccggc gatcgcatcg aacccacctt tggcgatgat ccggccgccg acgacgaagc
      241 ggtgttcgat gcgcagcagg gcgaactcga ggtcggcatg cgcggcgaat tgcagcgtct
      301 gggttcggcg atcgctgcca aacgtggcga agatggcaaa ggggtggtcg cgaaaccggc
      361 cgccggtacg cgcccggagc aaccgatcga gcgtatcgtc acgttgttcg tcgccgcacc
      421 ggcggaagcg gatttccacg gtcccgacct cgtcgtcgcg gctgaaaaag ccgggctgga
      481 tttcggcgac ctcggcattt tccaccggct ggtatcgggc aagcccgagg ctggtccggt
      541 cttcagcatg gcgaacatgg tcaagcccgg cagtttcgac atgacgcaga tcgaatcgct
      601 gcgtacgccg ggcgtgagtt tcttcatgac cctgcccgga ccgatgtcgg cgctagacgc
      661 ctgggaagcg atgctgccca cggcgcagcg gctggccgag ctgctcgacg gtcgcgtgct
      721 cgacgaaggc cgcaacgcgc ttggccgcca gcgcatagcg cacctgcgcg acgagctgcg
      781 tgcctgggac cgcaagcagg aacgcaacca gatccgtccg cgataaggcg tacacacacg
      841 atgatttcag catccgaagc cctcgaacgc ctgcgcgaag gcaaccgccg tttcgttgcc
      901 gacgattgca atggcggcca gcaggccggc aagaaccgtc gccgtgaact ggcggagggg
      961 caggaaccgt tcgcgacgat cctcggctgt tccgattcgc gcgtcccggc ggaaatcatc
     1021 ttcgaccagg gcctgggcga cctgttcgtg attcgtgttg ccggcaacat catcgcgccg
     1081 tcgctggtcg gcagcgtcga gttcgctgcc gcgcgcttcg gcacgccgct ggtcgtcgtg
     1141 cttggccata cccagtgcgg cgcgatcgct gccactatcg aggagttgac ccagcagggc
     1201 ggggcgcagt caccgaacct gcagtccatc gtcaatcgcg tgcgtccgag cgtgctgccg
     1261 ttgatgaaca ccgagttgcg ccacgacctg catgcactca gccgcgaggc cgtgcgcgcg
     1321 aacatccgcg cctcgaccaa ccatctgcgt cacggttcgg aactcatgga aaacctgatc
     1381 gaagaaagag gttttcgcgt ggtgggtgcg gagtattcgc tggagacggg gttggtcgat
     1441 ttcttcgacc ttggttagtg cgatcgtgag tagtgtgttt gttctagccg gcgcggctag
     1501 atttgcctca acgccgcatc gcggcggtta cccgaccaag aggcagaaca tggctaacaa
     1561 gcgcaacaag aatcaaacga cttcccccgc cggcaaggcg catgccgtgt ggctggccag
     1621 tctcggtgcg gtttccatcg tgcagaagcg gggcacggaa gttctctccg gtctcgcggc
     1681 cgaagggcgc gatttccagg cccgcgcgca acagctcgtg cgtgaagtcg gcgccgatgc
     1741 caaggtgcac gtgaagggcg tggtcgcgcc gctgcgcgtc gacctcaagc gcaacgccgc
     1801 caagttcggc gtggtggtgc agaacgcggt cgccggcgtg ctggcgaagc tcggcattcc
     1861 gtccaaggcc gacatcgaag cgctgagcca gcgcatgagc gcgctgagcc gccagctcaa
     1921 ggccgccaag taagaattca cactcccaac gaagcattgc ggccgccttg agcggccgtt
     1981 tttttatctg cggtaggctt gggccagcgt gttcagatgc gcgcgctcgg catcgcgcag
     2041 gaacggcgcg atcagcagca ttacctgata gatgccctgg ctgaggtcgt tctgggccgg
     2101 ctcgggattt gcgccgcgca cggcgtggta gttgagccag aacgtggcca ccagcaagac
     2161 gttttccgcc gtggcatgga tttcgtcctg ggtcgcgcgc aggatttctg ccttggccag
     2221 gccattgagc acggcttcgg cgctggccgc ggcgcggttg aggatgcgcg cgaagcgctg
     2281 ccgcaacttg cggttgcggg caaggatgtc gacgaggtcg cgatacagga atcggtattc
     2341 ccacatgcac tcgaagacca ggtgcagttg cagccagacg tcctcgaggt tcggcaggcg
     2401 accttccggc gcaaccagcg cgtcgtccat gcgcgcctcg tagcgcgcaa acaggcgctc
     2461 gacgatgtcg tccttgttgc ggtaatggta gtagaggttg cccgggctga tgccgagctc
     2521 atcggcgatg tggttggtgg tgacgttcgg ttcgccctgg gcgttgaaca tcgccaggct
     2581 ggcgtcgaga atgcgttcgc gtgtttgctt cgccgccatt cggaatgcct ttgcgaacct
     2641 acctcagaat cgtcgcgagc gaagatggtt tgtgtttcgc cggagcgcag tagattctga
     2701 gataggttgg tcagccgcgc aatttcttga ccaggtcgac gtatttctgc atcgcctcgt
     2761 cggccttcat gcccttgagc ttggcccagg cttcgtattt ggccgtgccg acgaagtcga
     2821 agaaacccgg tttggggccg gagacatcgc catcggaacc ctgcttgaac agtgcgtaga
     2881 gcttgagcag ggtgtcattg tcggggcgtt cgtcgagttt cttgacgtct tcggcggctt
     2941 gcgtgaactt ggttttcagg tcggccatgt cgttccccga tgcgattgca tgcgctgcgg
     3001 gcgtggacaa tggcaaaagc gaacgaacgt gtcaaacggc cgttggcttt gctatctttg
     3061 catcctgcat cgcggggata cacaccatga cctatttcgt gacgggcgcg accggtttca
     3121 tcgggcgcca tctgctggaa ctcctgctcg cgcgcaaggg cgacgtgcat tgcctggtac
     3181 gcaagggttc gcagagaaaa ttcgaagaag tgcgcgaacg tttcggcgcc gaaggcaagc
     3241 gcctgattcc cgtggtcggc gatctgacca agccgatgct gggtttgacg ccggcgcagc
     3301 gcaaggcgct ggcgggaaag gtcaaacatt tcttccatct ggccgcgatc tacgatctcg
     3361 ccgccaatgc agacagccag atcgcggcga acgtcgacgg cacgcggcac gcgctggaac
     3421 tcgcggacgc actgaaggcc ggctgcttcc actacgctag ttccattgcc gttgccggct
     3481 ttttccccgg tgtttttcgc gaagacatgt tcgccgaggc ggagaacctc gatcatccgt
     3541 atttccgcac caaacacgat gccgaggcgc tggtgcgcgc ggcgacgatc aagcatcgca
     3601 tttatcgtcc ggcgatcgtg ctgggcagtt caaaaaccgg cgagatcgac aagatcgacg
     3661 ggccgtatta tttcttccgc gcgctcaaga agctgcgcga agcgttgccg gcgtggatgc
     3721 cgacgattgg ccttgaaggc ggccgcgtaa acatggtgcc ggtggatttc gtcgtcgcgg
     3781 ccatggatca cattgcacac aagccgggcc tggacggcgg ctgtttccat ctcaccgacc
     3841 cggcgccgcg gcgtatcggc gaggtgctca acatcttcgc ccgcgccggg cacgcgccgc
     3901 agatgaccat gcgcctggac gcgcgcatgt tcgggttcat cccgccgatg atcaagcagg
     3961 cgctggcggg cctgccgccg gtcaagcgct tcaccggcgc ggtgctgcgc gacctcggca
     4021 ttcccgccgc cgcgatgcgc atgatcaact atccgacgcg tttcgattgt cgcgagacag
     4081 agcgtgcgct caagggcacg aagatccgcg taccggacct gcagggttac gcctggcggt
     4141 tgtgggacta ttgggagcgc cacctcgatc cggatttgtt cgtcgaccat tcactgtcgg
     4201 gcaaggtgcg tgggcgcacg gtgttgatta ccggtggctc atccggcatc ggcaaggcgg
     4261 cggcattgaa gatcgccggc gcgggagcga aagttctgat ctgcgcgcgc ggcgaggacg
     4321 agctcaatgc cacgcgcaag gaaatcgtcg atgcgggcgg cgtatgccac acctatgttg
     4381 ccgatctggc cgaccagaaa tcctgcgacg agctggtgca gaaggtactg gccgagcaca
     4441 agggtgtgga tatcctgatc aacaatgccg ggcgttcgat ccggcgcgcg attgcgtcca
     4501 gctacgaccg cttccacgat ttcgagcgga cgatgcagct caattacttc ggctcattgc
     4561 gcctgatcat gggctttctg cctggcatgg cggcgcgcca tcgcggccac atcatcaata
     4621 tttcgtccat cggcgtgctg acgaatgcgc cgcgcttttc cgcctacgtt tcttcgaagg
     4681 cggcgctgga cgcgttctcg cgctgtgcac aggccgaatt ttccgacaat ggcattcatt
     4741 tcaccacgat caacatgccg ctggtgaaga cgccgatgat cgcgccgacc aagatgtatg
     4801 aatcggtgcc gacgctgtcg cccgacgaag ccgccgacat gatcgtggac gccatcatcc
     4861 agcgtccggt gcgcatggcc acccgattgg gcacgttcgc gcagatcttc tatgcgctgg
     4921 cgccgcgcgc ctatgaaatc ctgatgaata cggcgttccg cctgttcccg gattcggcag
     4981 ccgccaaggg catgaaggga cgcggcgagt tggacaagca gccgaccagt gagcaggtcg
     5041 cgtttgcgtc cttgatgcgg ggagttcact ggtagaaaat cgcacgttca tgcgatgcct
     5101 tgaatggcat cgcatgtgag attttctggt gagccatgga tggcgaacgg aaaagcgcca
     5161 cggatggcgc ccacgcgaaa aattacccgt aacgaattgc gcgcggtgtt ttcaggcgag
     5221 gcagtcggga ttactcgatc ttgatcggct ccttcccagc cgccttcagc ttggcattca
     5281 acgccaccag atcgtgtgat ttcagttcgt cccacgaatt cagttccgca tcgaccatcg
     5341 gcagcagtgc cttgaagccg ttttgcgcgt cgacggatgg cgcggcgtcg gcacccgcca
     5401 ccgcggcctg cagtttggcg agggcgtcgg cgaggaattt gaagttgcgc gtatcgcgcg
     5461 gcgggtaggc ccaggcatta cgcggattcg gcgcttcgcg tgcgccggtg attgccgccg
     5521 tgcgttcaag cagcccgccg atttcacggt caagctcctt gtccggtacg cgtgctttct
     5581 tcaaggcttg aatcagcgcg ccagcctcgc gcgcggcaac ggagacgcga gtgttttgcg
     5641 cctggattga ttttgccagt gcgaactgcc gtgcataagc cgaagcgggc agattcacac
     5701 gtggatcggc cactacctgc agcggttgtt cctgcttgac cccatccacg gtcagcacga
     5761 cgcggtaatc gccgggtggt gcaaacaggc cgtccgccca gggatcggac ttctcacccg
     5821 atggtggcgc cgggtattgc agcggccaga cgaagcgatg ggtacccgtg gcagcggaca
     5881 aggtgctggg gacatccgcc cattccggcg ctatcggcga cttcgtcgga tcgacggccg
     5941 cgaccgggtc ggcgctggaa taatgtcgaa ccagcgcacc ggtcgaatcg ataatatcca
     6001 gcgtaatcgg tgatttgatg gcgattttca atgtgtaatc tagataagcg cccagtggtg
     6061 gattttccgc catcggttcg tccttgggca tgggcgtacc ggtgaatgcc ggcagacgga
     6121 ctcggtaggc gggttctggt ttgaacagcc agaatgctgc tttttcgacc ttggcatcga
     6181 actggcgcag cgatgaagcg tcgtccatga tccagatgcc gcggccgtgt gtggcgagta
     6241 ccaggtctgc accgtgaatg gcgatgtcgc gcacggatgt gaccggcagg ttcttctgca
     6301 aagtctgcca gtgctcgccg tcgtcgaacg acgcgtagac gccgcgttcg gtgccggcat
     6361 acagcaggcc gcggcgtttc ggatcggcgc gtaccacgtt caccgactga ttcgacggga
     6421 tgccggcgac gacgagttgc cagctcttgc cgccgtcgcg ggtgcgatag atgtagggcc
     6481 gctgatcgtc gaggcgatgg cggtcgaccg ccgcgtaggc gctgtccgca tcgaatgcgg
     6541 acgcgtcgat gatgccgacc ttcgaccaag gcgtgagctg attcggcgtg acgttgtccc
     6601 agtgcgtgcc gccgtcgcgg gtgcgccaga tcagtccgtc gtcggtaccg gcccagatca
     6661 gaccgtcggc gacaggcgag ggcgcgagtg catagacgac gccacgacgc ggaccctggc
     6721 caagattgtc ggcggcggtg acggggtcga ggttttttgg cgtgcccgga ttttcgcgag
     6781 tgaggtcggg actgatggcc tgccagtgtt cgccgtggtc gtcggtgcgg aacacgcgct
     6841 ggttggcgaa gtagagcaca tgcggctggc gcggcgagaa caccagcggc agggtccaga
     6901 cgctgcggtt gagtccgggc agggccaagg tcggatcgac gctgcgcacc tgctgcgtac
     6961 gcaggtcgag cttgtcgacc cgcccgccaa acacggtttg cggatcgtcc ggatc
//
LOCUS       DASRVO010000194         1332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_291797, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000194 DASRVO010000000
VERSION     DASRVO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291797"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..552)
                     /locus_tag="VFF05_02315"
     CDS             complement(139..552)
                     /locus_tag="VFF05_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZW50672.1"
                     /translation="MENNRPVVVKRIPDRLNNRQAKIFLREVANIVNSDRPQVVFDCS
                     QVQQIDAAGVEMLLQCLARAIKHDGDLKLAAVSPQMEIVLQMTRTDRLFEFYDTAAAA
                     VLSFSRFLPSAVKRPISVLTMPTPAATDNAAEFAA"
     gene            complement(563..>1332)
                     /locus_tag="VFF05_02320"
     CDS             complement(563..>1332)
                     /locus_tag="VFF05_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50673.1"
                     /translation="IDNRTHATQAFARDGAPMDLFMVSASRRNEAAYLFRHHLRETRT
                     RCGSWRFRGYHNALETAQHVRCLAVDGLLRENSIEPDGREVRPGVWFARDARVHAQAR
                     ILAPAFVGERARIRANAVLTRCSVVEQRAEVDCGSVIEDATVLPYTYVGPGLDIMHSV
                     VGSSHIVNLEHDIEVAIADPRLLRELPASTGRRALSGAASAVALLPALLRNLIGSRRR
                     SAELPQAVSTPSAVDTPPGFPAGPSAMEASSLQRTWQ"
BASE COUNT          238 a          428 c          437 g          229 t
ORIGIN      
        1 agccggtcag catgatcacg cttccgccca gcacacgcga gcgcgtgctg ttgccgacca
       61 accgattccg cacagcaccg cgccagctac tctgatgagg cggagcggtc gtaatggcgc
      121 cggaatcagt catacgcttt aggctgcgaa ttcggccgcg ttatcggtgg cagccggcgt
      181 tggcatggtc aggaccgata tcggacgttt caccgccgat ggcaggaagc ggctgaaact
      241 gagcaccgcg gcggcggccg tgtcgtagaa ctcgaacagc cggtcggtgc gcgtcatttg
      301 cagcacgatt tccatctgcg gcgaaacggc cgccagcttg aggtcgccgt catgcttgat
      361 ggcgcgcgcc aggcactgca gcagcatctc gactccagcg gcatcgatct gttgcacctg
      421 cgagcaatcg aaaaccactt gcggccggtc ggaattaacg atgttggcga cctcgcgcag
      481 gaaaatcttg gcttggcggt tgttcaggcg atcgggaatt cgctttacga cgacgggccg
      541 attgttctcc atatctcctc aattactgcc aagtgcgttg tagggacgaa gcttccatgg
      601 cgctcgggcc agccggaaat ccgggcggcg tatcgaccgc cgagggcgtg gaaactgcct
      661 gcggaagttc cgcggagcgg cggcgcgacc caatcaagtt ccgcaacagc gcgggcagca
      721 aagctacggc cgaggccgca ccggagaggg ctcgccggcc ggtgcttgcc ggcaattcgc
      781 gcagcagtcg cggatcggcg atcgcgactt cgatatcatg ttccagattc acaatgtgcg
      841 aggagcccac caccgagtgc atgatgtcga gccccggccc gacataggtg tacggcagga
      901 ccgttgcatc ctcgatcacg ctgccgcagt ccacctccgc gcgctgctcg accacgctgc
      961 aacgggtcaa caccgcgttg gcgcgaatgc gggcgcgctc gccgacaaag gcgggcgcca
     1021 ggatgcgtgc ctgggcgtgc actctcgcgt cgcgcgcgaa ccacaccccc ggacgaacct
     1081 cgcggccgtc gggctcaatg ctgttctcgc gcagcaggcc gtccaccgcc aggcagcgta
     1141 cgtgctgtgc ggtctcgagc gcgttgtgat agccgcggaa tcgccaggac ccgcagcggg
     1201 tgcgcgtttc cctcaaatgg tggcggaaca ggtacgccgc ctcgttgcgg cgcgacgcgc
     1261 tcaccatgaa caggtccatg ggcgcgccat cgcgcgcgaa cgcctgtgtg gcgtgcgtgc
     1321 gattgtcgat gt
//
LOCUS       DASRVO010000195         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_188019, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000195 DASRVO010000000
VERSION     DASRVO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188019"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..902
                     /gene="asd"
                     /locus_tag="VFF05_02325"
     CDS             42..902
                     /gene="asd"
                     /locus_tag="VFF05_02325"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344026.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HZW50674.1"
                     /translation="MSNRLAVIPQYLLPKRVITVLAGKLANLRGGAFTHAVIRRFVAH
                     YRVDMSEAAQSRIEQYPTFNDFFTRPLRTGVRPIADAPFVCPVDASVSQFGPIEHDQI
                     FQAKGHSYSTRALVGGDQELAHRFDGGHFATLYLAPRDYHRIHMPCDGRLVRMIHVPG
                     DLFSVNPLTARHVPNLFARNERVVCVFDTAVGPFVNVLVGATIVGSVATVWHGVVNPP
                     PRAGIREWRYDDRSIALVKGQEMARFLLGSTVVMLFPKGVLTFVSDWAPSRPVRLGEP
                     MATAAAGPRD"
     gene            complement(906..>1530)
                     /locus_tag="VFF05_02330"
     CDS             complement(906..>1530)
                     /locus_tag="VFF05_02330"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HZW50675.1"
                     /translation="VGPANAKTITDHIAVLRPHAWMMLGHCAGLRNSQQLGDYVLAHA
                     YVREDHVLDEELPLWVPIPALAEIQLALERAVADVTQCKGADLKRIMRTGTVASTDNR
                     NWELLPGNMPQRRFSQSRAVALDMESATIAANGFRFRVPYGTLLCVSDKPLHGEIKLP
                     GMANQFYRERVDQHLRIGMRAIEILRAEGTQRLHSRKLRSFDEVAFQ"
BASE COUNT          233 a          510 c          500 g          287 t
ORIGIN      
        1 cggcaaatgg cagaattttc agtttccgac ctgggaggat catgtcgaac cgactcgcgg
       61 tgataccgca atacctgctg cccaagcggg tgatcaccgt gctcgccggc aaactcgcga
      121 acctgcgcgg cggcgcgttc acgcatgcgg tgattcgcag gttcgtcgcg cactatcggg
      181 tcgacatgtc ggaagcggcg cagtcccgga tcgagcagta cccgacattc aacgacttct
      241 tcacgcggcc gttgcgaacg ggcgtgcggc cgatcgccga tgcgccgttc gtatgcccgg
      301 tcgatgcctc ggtcagtcag ttcggaccga tcgagcatga ccagatcttc caggcaaagg
      361 gccacagtta ttcgacgcgc gcgctcgtcg ggggcgacca ggaactcgca caccggttcg
      421 acggcgggca tttcgccacg ctgtacctgg cgccccgcga ctatcaccgc atccacatgc
      481 cttgcgacgg ccggctggtg cgcatgatcc acgtgccggg cgatctgttc tcggtcaacc
      541 cgctcacggc gcgccatgtg cccaacctgt ttgcgcgcaa cgagcgcgtg gtctgcgtgt
      601 tcgacaccgc cgtcggtccg ttcgtgaatg tgctggtcgg agcaacgatc gtcggcagcg
      661 tcgccacggt ctggcatggt gtcgtgaacc cgccgcccag ggccggcatc cgcgagtggc
      721 gttatgacga tcgcagcatc gcgctcgtca agggccagga aatggcgcgt tttctccttg
      781 gttccaccgt cgtcatgctg tttccgaagg gcgtgctgac gttcgtgtcc gactgggcgc
      841 cgtcgcgccc ggttcgcctg ggcgagccga tggccaccgc ggcggctggc ccgcgcgact
      901 gatcgctact ggaaggcgac ctcgtcgaag ctgcgcagct tgcggctgtg caggcgctgg
      961 gtgccttcgg cgcgcaggat ctcgatcgcg cgcatcccga tgcgcaggtg ctggtccacg
     1021 cgctcgcgat agaactggtt ggccatccct ggcagcttga tctcgccgtg cagcggcttg
     1081 tcgctgacgc acagcaaggt gccatagggc acgcgaaagc ggaagccgtt ggcggcgatc
     1141 gtcgcgcttt ccatgtccag cgcaaccgcg cggctctggc tgaaacggcg ttgcggcatg
     1201 ttgccgggca gcagttccca gttgcggttg tccgtgctcg cgacggtgcc ggtgcgcatg
     1261 atgcgcttca ggtcggcccc cttgcattgc gtcacgtccg ccaccgcccg ctccagcgcc
     1321 aactggattt cggccagcgc cgggatcggc acccacagcg ggagctcctc gtccaggaca
     1381 tgatcctcgc gcacgtaggc atgagccagc acatagtccc cgagctgctg gctgttgcgc
     1441 agacccgcgc agtggcccag catcatccac gcatgcggcc gcaggactgc gatatggtcc
     1501 gtgatcgtct tcgcattggc cggtccgacg
//
LOCUS       DASRVO010000196         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_239086, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000196 DASRVO010000000
VERSION     DASRVO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239086"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /gene="gcvT"
                     /locus_tag="VFF05_02335"
     CDS             complement(<1..349)
                     /gene="gcvT"
                     /locus_tag="VFF05_02335"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003466929.1"
                     /note="catalyzes the transfer of a methylene carbon from
                     the methylamine-loaded GcvH protein to tetrahydrofolate,
                     causing the release of ammonia and the generation of
                     reduced GcvH protein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HZW50676.1"
                     /translation="MTQQTVLNATHRAHGAKMVDFGGWDMPLNYGSQIEEHHAVRRDA
                     GMFDVSHMTVVDLHGAGTRAFLRHLLANSVDKLKLPGKALYSCMLDDGGGVIDDLIVY
                     FLSEDFFRVVVNAA"
     gene            461..1432
                     /locus_tag="VFF05_02340"
     CDS             461..1432
                     /locus_tag="VFF05_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZW50677.1"
                     /translation="MSHDNHPENPQRRNALGCLAAWSGAAVVWTLAGGVPRALGATTA
                     GGIAAAAKNAFTFVQISDTHIGFNKPANPDVVGSLRRAIADINALPQAPGLVVHTGDV
                     SHLSRPEQFWQARELFGELHVSRVHTIPGEHDALDNGLDGYLKAFDHDGKHRAWYSFN
                     QGGVHFIGLSNLLDFRMGSMTSLGDEQIAWLKKDLQGVAHSTPIVVLAHVPMWMIYPQ
                     WSWGTADADQALALLRPYGSVTVLNGHIHQVMQKVEGNIAIHTAMSLAYPLPKPGQSG
                     FGEPGPVTVPAGELGKLIGTRQVTVVPGRHDLALIDTPLERVPANHS"
     gene            1429..1737
                     /locus_tag="VFF05_02345"
     CDS             1429..1737
                     /locus_tag="VFF05_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HZW50678.1"
                     /translation="MKLAAIALAFASLASPAIAADVRIDINKFAFTPREITVKPGTTV
                     VWSNDDQTPHTISAKDGSFASTGMDTGDAYQHTFTREGDVAYFCTVHPFMTGIVHVRQ
                     "
BASE COUNT          347 a          636 c          583 g          309 t
ORIGIN      
        1 tcgccgcgtt gacgacgacg cggaagaaat cctcgctcag gaaatagacg atcaggtcgt
       61 cgatcacgcc gccgccgtca tcgagcatgc acgagtacag cgccttgccg ggcagcttga
      121 gcttgtcgac gctgttggca agcagatggc gcaggaacgc gcgcgtaccg gcgccgtgca
      181 ggtcgaccac ggtcatgtgc gagacgtcga acatgcccgc gtcgcggcgc acggcgtggt
      241 gctcctcgat ctgcgagccg taattgagcg gcatgtccca gccgccgaag tcgaccatct
      301 tggcgccgtg cgcgcgatgg gtggcgttga gaacggtctg ctgggtcatg gcggcgaagg
      361 gcgaagaaag gggcgtcatt gtaacggact ggcgcgaccg ggaataaatc cgcgccgcgc
      421 acgatcttgt accgcggatc gtccaccacg gagcgcaatc atgagccacg acaaccatcc
      481 cgaaaatccg caacgtcgca acgctctcgg ctgcctcgcc gcctggagcg gcgccgccgt
      541 ggtctggacc ctggccggcg gcgtgccgcg cgcgctgggc gcaaccacgg ccggcggcat
      601 cgcggcggcc gcgaagaacg ccttcacctt cgtccagatc agcgacaccc acatcggctt
      661 caacaagccg gccaatccgg acgtcgtcgg cagcctgcgc cgcgccattg ccgacatcaa
      721 cgcgctaccg caggcgccgg gcctcgtcgt tcacaccggc gacgtcagcc acctgtccag
      781 gcccgagcag ttctggcagg cgcgcgaatt gttcggcgag cttcacgttt cgcgcgtcca
      841 caccatcccg ggcgagcacg acgcgctcga caatggactg gatggttacc tgaaagcctt
      901 cgaccatgat ggcaagcatc gcgcctggta cagcttcaac cagggcggcg tgcacttcat
      961 cggcctgtcc aacctgctcg atttccgcat gggctcgatg acatcgctag gcgacgagca
     1021 gatcgcctgg ctgaagaagg acctccaggg cgtggcacac tcgacgccga tcgtggtgct
     1081 ggcgcacgtg ccgatgtgga tgatctatcc gcagtggagc tggggcaccg cagacgcgga
     1141 tcaggcgctg gccctgctgc gcccctacgg atcggtcacc gtgctcaacg gccatattca
     1201 ccaggtgatg cagaaggtgg aaggcaacat cgccatccat accgcgatgt cgctggccta
     1261 ccctttgccg aaacccggcc agtcgggttt tggcgaaccg ggacccgtga ccgtgccggc
     1321 cggcgaactc ggcaagttga tcggtacgcg ccaggtgacc gtggtgcctg gccgccacga
     1381 tcttgccctg atcgacaccc cgctcgaacg tgtgccggcg aaccactcat gaaactcgcc
     1441 gccatcgcac tcgcctttgc ctcgcttgcg tcgccggcca tcgccgcaga cgtcaggatc
     1501 gacatcaaca agttcgcgtt cacgcccagg gaaatcaccg tgaaaccggg cacgaccgtg
     1561 gtctggtcga acgacgacca gacgccgcac acgatcagcg ccaaggatgg cagttttgca
     1621 tcgacaggca tggataccgg cgacgcttac cagcatactt tcacccgcga aggcgatgtc
     1681 gcttactttt gcacggtgca tcccttcatg accggaatcg tgcacgtgcg ccaatgagcc
     1741 ccagcgccaa cgccgccttc gtcgccgacc tcgcgcagcg tcatcttgac gcggcgtata
     1801 accttggccg ctggctgctc ggcaacgatc aggacgccgc cgatgcggtg cacgacgcat
     1861 tcgtgcgtgc gctgc
//
LOCUS       DASRVO010000197         1185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_109439, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000197 DASRVO010000000
VERSION     DASRVO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109439"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFF05_02350"
     CDS             complement(<1..824)
                     /locus_tag="VFF05_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZW50679.1"
                     /translation="MTKTQAQASASIPSTLRRLAPYLREHGWRIVISLALLIVAKIAT
                     IGVPLSLKAIVDHLDPRVALLTVPIAMIAGYGALRLIGTLFGQLRDTVFERVAQRAMR
                     ASGLDAFRHLHALSLRFHLERHTGGVGRDVSRGTRGVYNLLGWVVFNIVPTVVEIGLV
                     TALLLRELDWRYAAVTLATMVVYIAATIWISEWRTAGVRAMNEVESLSNARAVDSLLN
                     YETVKYFGNEDFEARRYDADLHRYEDAAVKTENSLALLNGAQALVIAAGLTALMAMA"
     gene            915..>1185
                     /locus_tag="VFF05_02355"
     CDS             915..>1185
                     /locus_tag="VFF05_02355"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HZW50680.1"
                     /translation="MRIAILAAATVLVSAAAFAKDAAKPMPSANPAPPHGARVFIESP
                     RNGATVGQDVHIVFGVSDIKVAPATDTSPGTGHHHLLIDVKELPPL"
BASE COUNT          216 a          397 c          388 g          184 t
ORIGIN      
        1 gccatcgcca tcagcgcagt caggcccgcc gcaatcacca gcgcctgcgc gccgttgagc
       61 agggccagcg aattttccgt cttcaccgcc gcatcctcgt agcggtgcag gtcggcgtcg
      121 tagcggcgcg cctcgaaatc ctcgttgccg aaatacttga ccgtctcgta attgagcagg
      181 ctgtccaccg cgcgcgcgtt ggacaggctc tcgacctcgt tcatcgcgcg cacgccagcg
      241 gtgcgccact cgctgatcca gatcgttgcg gcgatgtaca caaccatcgt cgcgagggtg
      301 acggcggcgt agcgccaatc cagctcgcgc agcagcaagg cggtgacgag gccgatttcg
      361 accacggtcg gcacgatgtt gaacaccacc cagccgagca ggttgtacac gccgcgcgtg
      421 ccgcgcgaga catcgcggcc gacgccgccg gtgtggcgct cgaggtgaaa gcgcagcgac
      481 agggcatgca aatgacggaa cgcatccagc cccgacgccc gcatcgcgcg ttgcgcgacg
      541 cgctcgaaca cggtgtcgcg caactggccg aacagcgtgc cgatcaggcg cagtgcgcca
      601 tagcccgcga tcatggcgat gggcaccgtg agcagggcga cgcgtggatc cagatgatcg
      661 acgatggcct tgagcgacag cggcacgccg atggtggcga tcttggcgac gatcagcagg
      721 gcgagggaga tgacgatgcg ccagccatgt tcgcgcaggt agggcgcgag ccgccgcagg
      781 gtcgagggaa tgctggcgga ggcctgggct tgagtttttg tcattgtcgc aaggtgtggg
      841 cggatcggct acgcacaagc cgaagcaggt gcatcagcgt atcatccgac gatcgccagc
      901 cacggagaag agtcatgcgt atcgccattc tcgccgccgc caccgtcctg gtttccgccg
      961 cggcattcgc caaggacgcc gccaagccca tgccatcggc caatcccgcg ccgccgcatg
     1021 gcgcgcgcgt gttcatcgaa tcgccgcgca acggcgcgac ggtcgggcag gatgtgcaca
     1081 tcgttttcgg cgtcagcgat atcaaggtcg caccggccac cgacacctct cccggcaccg
     1141 gccatcatca cttgctcatc gacgtcaagg aactgccgcc gctgg
//
LOCUS       DASRVO010000198         1255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_343228, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000198 DASRVO010000000
VERSION     DASRVO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343228"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /gene="tldD"
                     /locus_tag="VFF05_02360"
     CDS             complement(<1..1205)
                     /gene="tldD"
                     /locus_tag="VFF05_02360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HZW50681.1"
                     /translation="MSMTLLAQAERQLLQPGGFSSRDLERVFAQLMGPSIDAADLYFQ
                     HSRSESWMLEDGIVREGSHAIEQGVGVRAMSGEKTGFAYSDEIVLPQLLTAAQSARAI
                     AQSGNAGAGHALAARESKSLYAPIDPVESLANEDKIALLREVDKFARALDPCITQVIV
                     TLSATLDTVLVAAADGTLAADVRPLVRMNVNVIAERNGRRESGTAGGGGRYGYRELLA
                     NGRAHAYAREAVRAALVNLDSVPVPAGTMTVVLGPGWPGVLLHEAIGHGFEGDFNRKG
                     TSAFAGRLGEKVASELCTVVDDGTLPNRRGSLSIDDEGVPGECTILVENGVLKGYMQD
                     KLNARLMGMRSTGNGRRESFAHLPMPRMTNTYMLPGQSDPGEIISSVQRGLYAVNFGG
                     GQVDITNGK"
BASE COUNT          218 a          415 c          401 g          221 t
ORIGIN      
        1 aacttgccat tggtgatgtc gacctgaccg ccgccgaagt tcaccgcata gagtccgcgt
       61 tgcaccgaag aaatgatttc accaggatcg ctctggcccg gcagcatgta ggtgttggtc
      121 atgcgcggca tcggcaggtg cgcgaacgat tcgcggcggc cgttaccggt ggaacgcatg
      181 cccatcaggc gggcgttgag cttgtcctgc atgtagccct tgagcacgcc gttctcgacc
      241 aatatcgtgc attcgcccgg cacgccttcg tcgtcgatgg acagcgagcc gcgccggttc
      301 ggcagggtgc cgtcgtcgac cacggtgcaa agttcggacg ccactttctc gcccaagcga
      361 ccggcaaaag ccgatgtgcc cttgcggttg aaatcgccct cgaatccgtg gccgatggcc
      421 tcgtgcagga gcacgcccgg ccagccgggg ccaagaacca cggtcatcgt gccggccggc
      481 acgggcaccg aatccaggtt caccagcgcc gcgcgcaccg cctcgcgcgc atacgcgtgc
      541 gcacgaccgt tggcgagcag ttcgcgatag ccgtagcgtc cgccaccgcc ggcggtgccg
      601 gattcgcggc gaccgttgcg ttcggcgatc acattgacgt tcatgcgcac cagcggacgc
      661 acgtcggcgg ccaacgtgcc gtccgcggca gcgaccaaca cggtgtcgag cgtggcggaa
      721 agcgtgacga tgacctgggt gatgcaggga tcgagcgcgc gcgcgaattt gtccacttcg
      781 cgcaaaagtg caattttgtc ttcattcgcc agcgactcga ccgggtcgat cggcgcgtac
      841 agcgatttcg attcgcgcgc ggcgagcgca tggccggcgc ccgcgttgcc cgactgcgcg
      901 atcgcgcgcg ccgactgcgc cgccgtcagc agctgcggca gcacgatttc gtccgaatag
      961 gcgaaaccgg ttttttcacc gctcatcgcg cgcacgccga cgccctgctc gatggcatgg
     1021 ctgccttcgc gcacgatgcc atcctcgagc atccacgatt cgctgcgcga gtgctggaaa
     1081 taaaggtccg ccgcgtcgat ggacggaccc atcaactgcg cgaacacgcg ctcgagatca
     1141 cggctggaga atccgccggg ctggagcaat tggcgttcgg cctgggcaag caaggtcatg
     1201 gacattcgat ttccgtttgc aagattgaaa cgcctgcgtt atgcgccgct cccgc
//
LOCUS       DASRVO010000199         4585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_161581, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000199 DASRVO010000000
VERSION     DASRVO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161581"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFF05_02365"
     CDS             <1..501
                     /locus_tag="VFF05_02365"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HZW50682.1"
                     /translation="HIRSIDDLLQFTNATGFQFLHTDRVAADSVLAGWLNISVGTECI
                     HFHGIRYHRRTQSPYCLGEVYRRASWQGLPQGFSRMEDAMRHLLEEKFQERIGKVEQS
                     LSAVPITEDQAHELKVRADTPGFRSVRRYFDIKGRLVLVAVTLHPGHLFSYFSKYERI
                     DPSVRI"
     gene            533..2134
                     /locus_tag="VFF05_02370"
     CDS             533..2134
                     /locus_tag="VFF05_02370"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HZW50683.1"
                     /translation="MSEREFMEYDVVTVGAGPAGLAFAIRLKQLKPDIRVCVIEKASS
                     VGAQILSGAVIEPQPLNELLPEWSKNPPPICVPAAHDEFWWLRDATRATKLPTPPQMH
                     NRGNFIVSLGALCAWLAPQAEALGVEIYPGFAAAEPFFNPDGSVGGVRIGDMGVAKDG
                     THKPAYTQGIDIKAPITVLAEGCRGSVSKQLIAKFKLDANSDPQTYGIGIKELWQLPA
                     GRVQPGKIVHSVGWPLDSCTYGGSFLYHLDKDRVAIGFVAGLDYDDPEFKPWEAFQQL
                     KNHARIKSLLEGGQILSAGARALIEGGWQSLPKCEMPGAILIGDAAGLMNVPKIKGTH
                     QAMRSGMLAAEHIVATSKTDGWDAKLRASAVMTELRKVRNIRPGFRKGFVAGMLNAGW
                     ETVTAGLSPWTLRNHADYSSLEKLGTFEVPKRGWIERTLPPRDRLASVYFASTQHDED
                     QPVHLKVPDTNICIGKCVVEYQNPCTRFCPAGVYEMVGEGASLRLQINAANCVHCKTC
                     DIKDPYENIIWTTPEGGSGPNYQNL"
     gene            complement(2156..3229)
                     /locus_tag="VFF05_02375"
     CDS             complement(2156..3229)
                     /locus_tag="VFF05_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HZW50684.1"
                     /translation="MRLLPFALFALPIAAFVAPPARAQNPDPMDIRACTAIHGDAQRL
                     ACYDRATHRDDTVAAEQKLADEAVRKTGVFRQVKPETETVAAAPRSLLDSRWELESDS
                     KLGTFNLRSYQPVYVMPLFATSRRNSLPSSPNPVDTVTDKQHLDNIEAKFQLSLKTKV
                     WQGVFGDDGDLWVGYTQSSRWQVYNVPQSRPFRESDYEPEALLVFGTGYSVLGWNGRL
                     FDIGIDHQSNGRSDPFSRSWNRVVADFGFERGNWMVRLRPWWRIPEARNDDNNPDIAD
                     YVGRGEMQVLHFWNDQEFNLTLRHSLRGGNANHGSARLSWSFPLDGNLRGYVEMFSGY
                     GESLIDYNHAATYLGVGLSLLDW"
     gene            3362..4246
                     /locus_tag="VFF05_02380"
     CDS             3362..4246
                     /locus_tag="VFF05_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50685.1"
                     /translation="MPSHRIALVTASTARDLDEDLIPLESALHEAGADVAIAAWDDAS
                     IEWSDFDLALLRSTWDYTQRLPEFLAWCERTATRTRLHNPLPVLRWNTDKHYLRDLDK
                     AGVAIVPSAFVEPNENAQTALTDFLSAHRTDEFVVKPAIGAGSRDAQRYHADDRDAAL
                     AHVRRLLEGKRSVLLPPYLDRVDEHGETALIFFDGKFSHAIRKGPLLRRFEAPTRALF
                     AAEHITPRVPGASELALAERALAAIPYGTLLYARVDLIAARDGSPRVLELELTEPSLF
                     FAHAAGAAARFAKAILAR"
     gene            4302..>4585
                     /locus_tag="VFF05_02385"
     CDS             4302..>4585
                     /locus_tag="VFF05_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EtfB protein"
                     /protein_id="HZW50686.1"
                     /translation="MKILVGYKRVVDYNVRIQVKPDGSGVVTDGVKLSANPFDDIALE
                     EALRLREKGIATEVIVATIGPADAQAHLRNGLAMGANRAIHVVTNDAIQPL"
BASE COUNT          852 a         1523 c         1427 g          783 t
ORIGIN      
        1 cacatccgct cgatcgatga tcttctccaa ttcaccaacg cgaccggttt ccagttcctg
       61 cacacagacc gtgtcgccgc cgattcggtg ctggccggct ggctcaatat cagcgtcggc
      121 accgaatgca tccattttca cggtatccgc taccatcgtc gcacgcagtc gccgtactgc
      181 ctgggcgaag tgtatcggcg cgcgagctgg caaggcctgc cgcaggggtt ttcgcgcatg
      241 gaagacgcca tgcgccacct cctcgaggaa aaatttcagg agcgtatcgg caaggtcgaa
      301 caatcccttt ccgccgtgcc gatcacggaa gaccaggccc atgagttgaa ggtgcgcgcc
      361 gatacgccgg gctttcgcag cgtgcgccgt tatttcgaca tcaagggccg cctggtactg
      421 gtggccgtga ccttgcaccc cggccatctg ttcagttact tcagcaagta cgagcgcatc
      481 gacccgtcgg tgcggatctg agcgcgcggc aaattccgcg acaggactcc tcatgagcga
      541 acgcgaattc atggaatacg atgtcgtcac cgtcggcgcc ggtccggcgg gattggcgtt
      601 cgcgatccgg ctcaagcagc tcaagccaga catccgcgtg tgcgtgatcg agaaggcttc
      661 aagcgtcggc gcgcagattc tttccggcgc ggtgatcgaa ccgcagccgc tgaacgaact
      721 gctgccggaa tggagcaaga atccgccgcc gatctgcgtg ccggccgcac acgacgaatt
      781 ctggtggctg cgcgacgcga cccgcgccac aaagttgccg acaccgccgc agatgcacaa
      841 ccgcggcaat ttcattgtct ccctcggcgc gctgtgcgcg tggctggcac cgcaggccga
      901 agcgctgggc gtggaaatct atcccggttt cgccgcggcc gaaccttttt tcaaccctga
      961 cggctcggtc ggcggcgtgc gcatcggcga catgggcgta gccaaagacg gcacgcacaa
     1021 gcccgcctac acgcagggca tcgacatcaa ggcgccgatc acggtgctcg ccgaaggctg
     1081 ccgcggcagc gtgtccaagc aactgatcgc gaaattcaag ctcgacgcga acagcgatcc
     1141 gcaaacctac ggcatcggca tcaaggaact gtggcaactg ccggccgggc gcgtgcagcc
     1201 cggcaagatc gtgcacagcg tcggctggcc gctggacagt tgcacctacg gcggcagttt
     1261 cctgtatcac ctggacaagg atcgcgtcgc gatcggcttc gtcgccggcc tcgattacga
     1321 cgatccggaa ttcaaaccct gggaagcgtt ccagcagctc aagaatcacg cgcgaatcaa
     1381 atcgctgctt gaaggcggcc agattctgtc ggctggcgcg cgcgcgttga tcgaaggcgg
     1441 ctggcaatcg ctgccgaaat gcgagatgcc cggcgcgatc ctgatcggcg acgcggcggg
     1501 cctgatgaac gtgcccaaga tcaagggcac gcaccaggcc atgcgctcgg gcatgctcgc
     1561 ggccgaacac atcgtggcga cgagcaaaac cgatggctgg gacgcgaaac tgcgcgcctc
     1621 cgcggtgatg acggaattgc gcaaggtgcg caacatccgc cccggattcc gcaagggctt
     1681 cgtcgccggc atgctcaacg ccggctggga aacggtcacg gcgggtctct cgccgtggac
     1741 gctgcgcaat cacgccgatt attcgtctct ggaaaagctc ggcaccttcg aagtaccgaa
     1801 acgcggctgg atcgaacgca cgctgccacc gcgcgaccgc ctggcctcgg tgtatttcgc
     1861 gtccacccag cacgacgaag accaaccggt gcacctgaag gtgccggaca cgaacatctg
     1921 catcggcaag tgcgtggtcg aataccagaa tccctgcacg cgcttctgtc ccgccggcgt
     1981 ctacgagatg gtcggcgagg gcgccagcct gcgcctgcag atcaacgccg ccaactgcgt
     2041 gcactgcaag acctgcgaca tcaaggaccc ttacgagaac atcatctgga ccacgcccga
     2101 aggcggcagc gggccgaatt accagaattt gtgacgcgcg cccggaacgg gtggtttacc
     2161 agtccagcag cgacaggccc acgccgagat aagtcgcggc gtggttgtag tcgatcaggc
     2221 tttcgccgta gccagagaac atctcgacgt agccgcgcag gttgccgtcg agcggaaagc
     2281 tccagctcag gcgcgccgag ccgtggttcg cgttaccgcc gcgcagcgaa tggcgcagcg
     2341 tgaggttgaa ttcctggtcg ttccagaagt gcagcacctg catttcgccg cgcccgacgt
     2401 aatcggcgat gtccgggttg ttgtcgtcgt tgcgcgcctc gggaatgcgc caccagggac
     2461 gcagccgcac catccagttg ccgcgctcga agccgaagtc cgcgaccacg cggttccaac
     2521 tgcgcgagaa cgggtcgctg cgtccgttgg actggtggtc gatgccgatg tcgaacagac
     2581 ggccgttcca gccgagcacg ctgtagccgg tgccgaacac cagcagcgcc tcgggttcgt
     2641 aatcggactc gcgaaacggc cgcgactgcg gcacgttgta gacctgccag cgcgaggact
     2701 gcgtatagcc gacccacaga tcgccgtcat cgccgaacac gccctgccag acctttgttt
     2761 tcagactcaa ctggaacttc gcctcgatgt tgtccagatg ctgcttgtcc gtcaccgtgt
     2821 cgaccggatt cgggctggac ggcaggctgt tgcgccgact ggtcgcgaac aacggcatga
     2881 cgtagaccgg ctgatagctg cgcaggttga acgtgccgag cttgctgtcc gactccaact
     2941 cccagcggct gtcgagcagg gagcgcggcg cggccgccac ggtttcggtt tccggcttca
     3001 cctgccggaa cacgccggtc ttgcgcaccg cttcgtctgc cagcttctgt tcggccgcca
     3061 ccgtgtcgtc gcggtgcgtg gcgcggtcgt agcaggcaag tcgctgcgcg tcgccatgga
     3121 tcgccgtgca ggcgcggata tccatcggat cgggattctg cgcgcgcgcc ggcggcgcga
     3181 cgaaagccgc gatcggaagc gcaaacaggg cgaacggcaa caggcgcatc gctgactcca
     3241 tgatcgctgg cagagaattt ctgccgctgt tccattctat cgtggcgaga acgaccgccg
     3301 cattccagcc accacgatcg agttttggcg tataaagcgc gctttgcccg gattcgactc
     3361 catgccatcg caccgcatcg ccctcgtcac cgccagcacc gcacgcgatc tggacgaaga
     3421 cctgatccca ctcgaatccg cgttgcatga agccggcgcg gatgtcgcca tcgctgcgtg
     3481 ggacgacgcc agcatcgaat ggtcggactt cgatctcgca ctgttgcgct cgacctggga
     3541 ttacacgcaa cgcctgccgg aattcctcgc ctggtgcgag cgcacggcga cgcgcacgcg
     3601 cctgcacaat ccgctgccgg tgctgcgctg gaacaccgac aagcactatc tgcgcgatct
     3661 ggacaaagcc ggcgtggcca tcgtgccgag cgcattcgtc gagccgaacg aaaacgcgca
     3721 aaccgcgctc acggattttc tgtccgcgca tcgcaccgac gaattcgtgg tcaagcctgc
     3781 catcggcgct ggctcgcgcg acgcgcagcg ctaccacgcg gacgaccgtg acgctgcgct
     3841 cgcgcatgtg cggcgcctgc tcgaaggcaa gcgcagcgtg ctgctgccgc cttacctgga
     3901 ccgcgtcgat gaacacggcg aaaccgcgct gatttttttc gacgggaaat tttcccacgc
     3961 cattcgcaag ggcccgctgc tcaggcgctt tgaggccccg acgcgggcgc tgtttgccgc
     4021 tgaacacatc acgccgcgcg tgccgggtgc gtccgagctc gcgctcgccg aacgcgcgct
     4081 ggcagccatc ccgtacggaa ctttgctgta tgcgcgcgtc gacctgatcg cagccaggga
     4141 cggttcgccg cgcgtgctgg aactggaatt gaccgagcct tcgctgttct tcgcccacgc
     4201 agcgggcgcg gccgcgcgct tcgccaaggc gatcctggcg cggtagaatc cgcattcctt
     4261 tcgcccgcac acgcgggccg cagcatcggg aaagcggaaa catgaagata ctcgtgggct
     4321 acaagcgcgt cgtcgattac aacgtgcgca tccaggtcaa gccggatggc tccggcgtcg
     4381 tcacggacgg tgtcaagctg tccgccaacc cgttcgacga catcgcgctg gaagaagccc
     4441 tgcgcctgcg cgagaagggc atcgccacag aagtgatcgt cgccacgatc ggcccggccg
     4501 atgcccaggc gcatctgcgc aatggcctgg cgatgggcgc gaaccgcgcg attcacgtcg
     4561 tcaccaacga tgccatccag ccgct
//
LOCUS       DASRVO010000200         1432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_32219, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000200 DASRVO010000000
VERSION     DASRVO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32219"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFF05_02390"
     CDS             <1..589
                     /locus_tag="VFF05_02390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZW50687.1"
                     /translation="SGRAQGAARSAADRDLSIMSETLTLARPYARAAFASAQASNALA
                     DWSRSLDVAARIVADPRVHSLVGDPRLGNAEVTALLLPPDVAADSPFGKFVVLLVENR
                     RADLLPDITALFEEFKREAERVLKVTVRSAVPFANGQADAIRAALKKRFGRDIELEQR
                     LDPSVIGGAVIDAGDMVIDGSVRGRLERLESALTQ"
     gene            735..>1432
                     /locus_tag="VFF05_02395"
     CDS             735..>1432
                     /locus_tag="VFF05_02395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161761.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZW50688.1"
                     /translation="MATTLNPSEISELIRNRIEKVKLAAEARNEGTIVSVSDGIVRIH
                     GLADVMAGEMIELPGNAFALALNLERDSVGAVVLGDYEHLREGDTVKTTGRILEVPTG
                     RELLGRVVDALGNPIDGKGPIQATERKRVDVKAPGIIPRKSVNEPMATGLKAIDALIP
                     IGRGQRELIIGDRQTGKTAVILDTFLNQKPAHAANASETDKLYCIYVAIGQKRSTVAQ
                     FVKVLEDNGALEYS"
BASE COUNT          270 a          494 c          458 g          210 t
ORIGIN      
        1 atccgggcgc gcacaaggcg ctgctcgatc agctgctgac cgagatctga gcatcatgag
       61 cgagacgctg acccttgccc gaccctacgc gcgtgccgcc ttcgcttccg cgcaggcgtc
      121 gaacgcgctc gccgactggt cgcgcagtct cgacgtcgcc gcccggatcg tcgccgatcc
      181 gcgcgtgcat tcgctggtcg gcgacccgcg cctgggcaac gccgaagtga ccgcactgct
      241 gctgccaccc gatgtcgccg cggattcgcc gttcggcaag ttcgtggtgc tgctggtgga
      301 aaaccggcgc gccgacctgc tgccggacat caccgcgctg ttcgaggagt tcaagcgcga
      361 agccgagcgt gtcctcaagg taaccgtgcg cagcgccgtg ccgttcgcca acggccaggc
      421 cgatgcgatc cgcgcagcgc tgaagaagcg cttcggccgc gacatcgaac tcgagcagcg
      481 gctcgatccg tcggtgatcg gcggcgccgt gatcgacgcc ggcgacatgg tgatcgacgg
      541 ctcggtgcgg ggaaggctgg aacgtttgga gtcggcgttg acgcaataaa ttcaagagcc
      601 ggtagccggt agccggagag cgaagagaac cgcgctcttg atctccggct accggcttcc
      661 ggctcccggc taccgaaaac agattcgatt gaaccccgcg catgcgaatg cctgcgcgaa
      721 ccaggaaccc aaagatggcc accacactca acccctccga aatcagcgaa ctgatccgca
      781 accgcatcga gaaggtcaag cttgccgccg aggcgcgcaa cgaaggcacg atcgtgtcgg
      841 tgtcggacgg catcgtgcgc atccacggcc tggccgacgt gatggccggc gaaatgatcg
      901 agctgccggg caacgcattc gcgctggcgc tgaacctcga gcgcgactcg gtcggcgcgg
      961 tcgtgctcgg cgactacgag cacctgcgcg agggcgacac ggtcaagacc accggccgca
     1021 tcctcgaagt gccgaccggg cgcgaactgc tcggccgcgt cgtcgacgcg ctcggcaatc
     1081 cgatcgacgg caagggcccg atccaagcga ccgagcgcaa gcgcgtcgac gtcaaggcgc
     1141 ccggcatcat tccgcgcaag tccgtcaacg agccgatggc cacgggcctc aaggcgatcg
     1201 atgcgctgat cccgatcggt cgcggccagc gcgagctgat catcggcgat cgccagaccg
     1261 gcaagaccgc tgtcatcctc gacaccttcc tcaaccagaa gccggcccac gccgccaacg
     1321 cgtccgagac cgacaagctc tactgcatct acgtggccat cgggcagaag cgctcgaccg
     1381 tcgcccagtt cgtcaaggtg ctggaagaca acggcgcgct cgaatattcg at
//
LOCUS       DASRVO010000201         1692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_109713, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000201 DASRVO010000000
VERSION     DASRVO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109713"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFF05_02400"
     CDS             complement(<1..270)
                     /locus_tag="VFF05_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HZW50689.1"
                     /translation="MLEQFGPSLYVAEGPIVSFFGFPYPTRMAVARLADGNVWVWSPV
                     ALTPELADAVNAIGPVRHIVSPNKLHHLFLHEWVEHWPDARLYAPP"
     gene            complement(292..1074)
                     /locus_tag="VFF05_02405"
     CDS             complement(292..1074)
                     /locus_tag="VFF05_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZW50690.1"
                     /translation="MTTNSARWRLDGQTALITGASRGIGLACAREFAALGADVLMVAR
                     DEAHLESARAELAEEFPDRDILAFAADVADHEQRLEVFDWIADLDTALSLLVNNAGTN
                     LRKPTLDYDDAEVRALIETNLLSAFAMCRLAYPHLVEHASAAIVNVGSVSGMTHVRTG
                     SPYGMTKAALGQLTKNLACEWAEDGIRVNAVAPWYIRTQRTEGTLADPDYLDEVLQRT
                     PLARIGEPEEVAAAAAFLCLPAASYITGEIVAVDGGFLKYGF"
     gene            1145..1375
                     /locus_tag="VFF05_02410"
     CDS             1145..1375
                     /locus_tag="VFF05_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50691.1"
                     /translation="MKIATAALAVVVLGLSACGADAPPPPKAAAAQPAQPLPDSNVFS
                     SDVKALQKAKDVQKTVDEQKKEQDKAIEDSGG"
BASE COUNT          317 a          551 c          546 g          278 t
ORIGIN      
        1 cggcggtgcg tagagccgcg catccggcca atgttccacc cattcgtgca ggaacaggtg
       61 atgcagcttg ttcggtgaga cgatgtggcg caccggtcca atggcgttta ccgcgtcggc
      121 aagctccggc gtgagcgcga cgggcgacca cacccagacg ttgccgtcgg caaggcgcgc
      181 gacggccatg cgcgtgggat acggaaaacc gaagaatgaa acgattggtc cttccgcgac
      241 gtaaagcgac ggtccgaact gttcgagcat gtcgaaagat tacacctgtc gtcaaaatcc
      301 gtatttcagg aatccgccat ccaccgcgac gatctcgccg gtgatgtaac tcgccgccgg
      361 caggcacagg aacgcggcgg cggcggcgac ttcttcgggc tcgccgatgc gcgcgagcgg
      421 cgtgcgttgc agcacttcgt ccaggtaatc cgggtccgca agcgtgcctt cggtgcgctg
      481 cgtgcggatg taccacggcg cgaccgcgtt cacgcggatg ccgtcttcgg cccactcgca
      541 ggcgaggttc ttcgtcaatt gcccgagcgc ggccttggtc atgccgtacg gcgagcccgt
      601 gcgcacgtgc gtcatgcccg ataccgagcc cacgttcacg atcgcggcgc tcgcgtgttc
      661 gaccagatgc ggatacgcga gccggcacat cgcgaacgcg gacagcaggt tcgtttcgat
      721 cagcgcgcgc acttccgcgt cgtcgtaatc cagcgtcggc ttgcgcaggt tcgtgccggc
      781 gttgttcacg agcagcgaca gcgcggtatc gaggtcggcg atccagtcga acacttccag
      841 gcgttgctcg tggtcggcaa cgtccgccgc gaacgcaagg atatcgcgat cgggaaattc
      901 ctcggccagt tccgcgcgcg cggattccag atgcgcctcg tcgcgcgcga ccatgagcac
      961 gtccgcgccg agtgcggcga actcgcgcgc acaggccagg ccaatgccac ggcttgcgcc
     1021 ggtgatcagc gcggtttgac cgtccagacg ccagcgcgcg gaattcgtcg tcatcgcggt
     1081 cctcgattgc ttgcggtacg catggctccg gcgcagactt tacgcattcg tacgggagac
     1141 tgccatgaaa atcgcaaccg ccgcgcttgc tgtcgtcgtc ctcggacttt ccgcctgcgg
     1201 cgccgacgca ccgccgccgc caaaggccgc cgccgcgcaa cccgcgcagc cgctgccgga
     1261 ttcgaacgtg ttcagcagcg atgtgaaggc cctgcaaaaa gccaaggacg tgcaaaagac
     1321 cgtcgacgag cagaagaagg agcaggacaa ggctattgag gattccggcg ggtgagatcg
     1381 agcgtttgcg cgatgcgatg aaacgcatcg cgcgcaagat cgaacgccgc gagccggagc
     1441 gagcagccgg cttgcttggc gagcgctgga cgcgcgagcc tggcaacagc ccgagcgcag
     1501 cgagacagac cgcctacctc atcgaacaaa agcaatacgc atacacaccc agcttgccgt
     1561 cgcgcgcact ttccagcaaa cgcaactgcg cgcggatctg cggctggtcg agcaggccga
     1621 gcggcatctg cggcagcacc gcctgggtga aactcacgag tgcgtcgttg ctcatgttca
     1681 tcgcaatccg ct
//
LOCUS       DASRVO010000202         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_213744, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000202 DASRVO010000000
VERSION     DASRVO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213744"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VFF05_02415"
     CDS             complement(<1..1632)
                     /locus_tag="VFF05_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW50692.1"
                     /translation="MARILIIDDRPVNRAFLNALLRQVNHEVIEAADGAAALALVRER
                     RPDLIITDLMLAPTGGVEFAMRVRDDAAIAGTPIIFYTATYRTDEALDLARRCGAVAV
                     LIKPFDPQEVLDVVATAIEARGNATPALSPLEQAPEAPGIELPGYVKDITGLQRDMRQ
                     QLRGAVAAIAGQTGSQSAAHIGVDTILPVAERLSAMLELDIALANENETGAMIALFCR
                     SARELLRCRRVALAVLDETGRGLRHLEMSGFDQGAAAQVTAAALASRAIDAMLESGKP
                     LRVADAEAARQIGLPEAPLGGDVLLLAPIPMRASTRAVGWLICSGSLDRPAFDAEDER
                     FLVAITAQFAIAHGNLMRLKDASRHAEALEAEVTRHHTTLMALRESEDRFRQIAANVD
                     AVFLLVTADFSWVHYVNPAYERIWGRPCASLYARARSWLDAIHASEREAIVDTIVGRS
                     SAGLGFVVETRIQHPDGSERWIHMSGSPISAGASTEARTAVVASDVTATKLAIAASRE
                     SERRLKEMLDNAELIALMLDHRGRIIYCNECFARLT"
BASE COUNT          292 a          631 c          669 g          316 t
ORIGIN      
        1 ggtcaggcgg gcgaagcatt cgttgcaata gatgatgcga ccgcggtggt cgagcatcag
       61 cgcgatcagt tccgcgttgt cgagcatttc cttgagccgg cgctcgcttt cgcgcgaagc
      121 ggcgatcgcc agcttggtcg cggtgacgtc gctggcgacg accgccgtgc gtgcctcggt
      181 gctggctccg gcgctgattg gcgagccgct catgtggatc cagcgctcgc tgccgtcggg
      241 gtgctggatg cgcgtctcga ccacgaagcc caagcctgcc gaactgcggc cgacgatggt
      301 gtcgacgata gcctcgcgct cgctcgcatg tatcgcatcg agccagctgc gggcgcgggc
      361 atacagcgag gcgcacggcc gtccccagat gcgctcgtag gcgggattga cgtagtgcac
      421 ccacgagaag tcggcggtga cgagcaggaa cacggcatcc acgttggccg cgatctggcg
      481 gaagcggtct tcgctttcgc gcagcgccat cagcgtggtg tggtgccgcg tcacttccgc
      541 ctcgagcgcc tcggcatggc gactggcatc cttcagccgc atcagattgc cgtgcgcgat
      601 ggcgaactgg gccgtgatcg cgaccaggaa gcgctcgtcc tcggcatcga aggcaggccg
      661 gtccagcgac ccgctgcaga tgagccagcc gaccgcgcgc gtcgatgccc gcatcgggat
      721 cggtgcaagc aggaggacat cgccgccgag cggcgcctcc ggcaggccaa tctgccgcgc
      781 agcttccgcg tccgccacgc gcagcggttt gcccgactcg agcatggcgt cgatcgcgcg
      841 cgaagccagg gccgcggccg tgacttgcgc cgccgcgccc tggtcgaatc cggacatttc
      901 gagatgacgc agtccgcgcc cggtttcgtc gagcacggcc agcgccaccc ggcggcagcg
      961 cagcagttcg cgggcgctgc ggcagaacag cgcgatcatc gcgccggttt cgttctcgtt
     1021 cgccagcgcg atgtcgagtt cgagcatggc cgacaggcgc tcggcgactg gcaggatggt
     1081 gtcgacgccg atatgcgccg ccgattggct gccggtctgc ccggcgatgg ccgcgaccgc
     1141 gccgcgcaac tgctggcgca tgtcgcgctg caggccggtg atgtctttca catagcccgg
     1201 caactcgatg ccgggcgctt ccggcgcctg ttccagcggc gataacgccg gcgttgcgtt
     1261 gccgcgcgcc tcgatcgccg tggccacgac atccagtact tcctgcggat cgaacggctt
     1321 gatcaacacg gccacggcgc cgcatcggcg ggcaaggtcg agcgcttcgt cggtacggta
     1381 cgtcgccgtg tagaaaatga tcggcgtgcc ggcgatggcg gcatcgtcgc gcacgcgcat
     1441 ggcgaattcg acgccaccgg tcggcgccag catgaggtcg gtgatgatca ggtcgggacg
     1501 gcgctcgcgc accagtgcca gtgccgccgc gccgtcggcc gcctcaatga cttcgtggtt
     1561 gacttggcgc aggagtgcgt ttagaaatgc ccgattgacc ggccgatcgt cgatgatgag
     1621 gattctggcc atggcgacac tcacatccga atcgaaacca gtgcacctgt cgcggcgagg
     1681 ctcgcgaggt tcgctgcggc acggacgcca acgcgagccg ctgcgcacag gcgagtgtaa
     1741 gcgcagaagg gcttcgggcg caccgggcat tcgatcgcaa gtgcgtgacg atcgtgcgtg
     1801 atccagccaa ttccgccggt ggcgtcaggc cgctggtttt cgtcgtggac gacgacgacg
     1861 gcatgcgcgc ggcagtgctc cgcgtcctcg gccgcgccgg aatcgacg
//
LOCUS       DASRVO010000203         1310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_188349, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000203 DASRVO010000000
VERSION     DASRVO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188349"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFF05_02420"
     CDS             complement(<1..517)
                     /locus_tag="VFF05_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HZW50693.1"
                     /translation="MNSATIARPRTDPALRDIDRRELYFFNLFRVLQAFVITGLMFSP
                     LVTSWVRLDHPLLGRLDGIVYLLVSGYALLRTEHWRHELRFKVAAMLTLDIAATTIAL
                     FALANPPSAIAMLLLVNIGAGALLLPPRQAGFFAALATLCVIGQSLASRAIDGSDRNL
                     IEYGLYGLSYFA"
     gene            653..>1310
                     /locus_tag="VFF05_02425"
     CDS             653..>1310
                     /locus_tag="VFF05_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010894968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZW50694.1"
                     /translation="MNFHEYQAKQLFADYGIPVPPGKIATTPDEAAAAARALGGDEWV
                     VKAQIHAGGRGKAGGVKLVKSPDEAKAAAAKMLGTKMATYQSGGRALPVNTVLVCEAT
                     DIAKELYLSVLVDRATKAISFIASARGGVDIEQVAHENPEDIHTLEVNFVEGVQPYQC
                     RKLGFAMGLNAKQANQLTKIMTGLYRLFNDKDLSLVELNPLAIVASGDLVALDGKVNS
                     D"
BASE COUNT          267 a          405 c          437 g          201 t
ORIGIN      
        1 tggcgaaata actcagcccg tacaggccgt attcgatcag gttgcggtcg ctgccgtcga
       61 tcgcgcggct ggcaagcgac tggccgatca cgcacaaggt cgccagcgcg gcgaagaatc
      121 cggcctgacg cggcggcagc aacagggcgc cggcgccgat gttgaccagc agcaacatgg
      181 cgatcgcgct tggcggattg gccagcgcga acagggcgat cgtggtcgca gcgatgtcga
      241 gggtcagcat cgcggcgacc ttgaagcgca actcgtggcg ccagtgctcg gtgcgcagca
      301 acgcgtagcc ggacacgagc aggtagacaa taccgtccag gcgtccaagc aggggatggt
      361 ccaggcgcac ccaactcgtc accaacgggc tgaacatgag cccggtgatc acgaacgcct
      421 gcagcacgcg gaacaggttg aagaaataca gttcgcggcg gtcgatgtcg cgcagcgcgg
      481 gatcggtgcg ggggcgggcg atggtcgcgg aattcacggt tggatcgagt ataccaatgc
      541 cgcgcgccac ggcgcggctt ttttttgccg gcgcgattgg ccacaatagg cgactttccc
      601 gccgtgccgg cgaatcttca gcaacgcggc gcggcttcgc ccgaggactg cgatgaactt
      661 ccacgaatac caggccaaac aattgttcgc cgactacggc atcccggtcc cgcccggcaa
      721 gatcgccacc acacccgacg aggccgctgc cgcagcacgc gcactcggtg gcgacgaatg
      781 ggtggtgaaa gcgcagatcc acgctggcgg ccgcggcaag gccggtggcg taaagctcgt
      841 gaaatcgccg gacgaggcga aagccgccgc ggcgaagatg ctcggcacga agatggcgac
      901 gtaccagtct ggcgggcgtg cgctgccggt gaacacggta ttggtgtgcg aggcgaccga
      961 cattgccaag gaactctact tgtcggtgct tgtcgatcgc gcgacgaagg cgatttcctt
     1021 catcgcgtcc gcgcgcggcg gcgtcgacat cgaacaggtc gcgcacgaaa atccggaaga
     1081 catccacacg ctggaagtga acttcgtcga aggcgtgcag ccgtatcaat gccgcaagct
     1141 cggcttcgcg atgggcctga acgcgaagca ggccaaccag ctcaccaaaa tcatgaccgg
     1201 gctgtatcgc ctgttcaacg acaaggacct gtcgctggtc gagttgaatc cgctcgcgat
     1261 cgtcgcgtcc ggcgacctcg tcgcgctcga cggcaaggtc aattccgacg
//
LOCUS       DASRVO010000204         1469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_369104, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000204 DASRVO010000000
VERSION     DASRVO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369104"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /gene="lepB"
                     /locus_tag="VFF05_02430"
     CDS             <1..713
                     /gene="lepB"
                     /locus_tag="VFF05_02430"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZW50695.1"
                     /translation="RETTRMNPLRRLLRILAENKSLLIFFGLMLCFRSAVADWMYVPS
                     GSMNPTIVEGDRILVDKEAYGLRIPFTTKRLTQGSDPQRGDIVIFASPEDGITLVKRV
                     IGLPGDTVEMRNEQLLINGSAIDYTPLPEVAADTELPSATHAQEHEYFTENLADHAHA
                     IMVLPQRHADRSFGPARVPAWQYLVLGDSRDNSKDSRYIGFVPRASIVGRAFAVAYSL
                     DADRWYRPRGDRLFKSLR"
     gene            complement(715..>1469)
                     /locus_tag="VFF05_02435"
     CDS             complement(715..>1469)
                     /locus_tag="VFF05_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW50696.1"
                     /translation="GGFIGSELAASLATNGCKVTMIFPGEAIGAGRYPPRLSEFLNAY
                     FREHGVELRAGAKVTGGKADAHAVDIALDDGTKLHFDALVAGLGVTPNVQLAEQTGLK
                     IDNGIVVDDRLRTSDPDIFAAGDVANFRNPALDMRLRVEHENAAIGMGHHAGRVMAGA
                     DEAYTTLPFFYSDLFDLGYEAVGLLDDRLDVVEFWTQPNREGVVYYLDDGRVRGVLLW
                     NVWGQVDAARELIAERGPHDADSLHGRLPKLA"
BASE COUNT          288 a          474 c          445 g          262 t
ORIGIN      
        1 agagggaaac gacgcgcatg aatccgttac gtcgcctgtt acgcatactc gcggaaaaca
       61 aatcgctgct gatctttttc ggcttgatgc tgtgcttccg ctcggccgtg gccgactgga
      121 tgtacgtgcc cagcggttcg atgaacccga ccatcgtcga aggcgatcgc atcctcgtgg
      181 acaaggaggc ctatggattg cgcattccat tcacgacgaa gcggctgacc caaggcagcg
      241 atccgcaacg tggcgacatc gtgatattcg cctcgcccga agacggaatc acactggtca
      301 agcgcgtgat cggactgccg ggcgataccg tggagatgcg caacgagcaa ctgctgatca
      361 atggcagcgc catcgattac acgcccctgc ctgaagttgc cgcggatacc gaattgccga
      421 gtgcgaccca cgcacaggaa cacgaatact tcaccgaaaa tctcgccgat cacgcgcacg
      481 cgatcatggt gttgccgcaa cgccacgccg accgttcgtt cgggccggcc cgtgttccag
      541 cctggcaata cctcgtgctc ggcgacagcc gcgacaacag caaggactcg cgctacatcg
      601 gcttcgtgcc gcgcgcgagc atcgtcggcc gcgcattcgc ggtagcgtat tcgctcgatg
      661 ccgatcgctg gtatcgaccg cgcggcgacc gcctgttcaa atcgctgcga tgagtcacgc
      721 gagcttcggc agcctgccgt gcaggctgtc ggcgtcgtgc ggaccgcgct cggcaatgag
      781 ttcgcgcgcg gcatcgacct ggccccagac attccacagc agcacgccgc gcacacgacc
      841 gtcgtcaagg taataaacga cgccttcgcg gttcggctgc gtccagaatt ccacgacgtc
      901 gaggcgatcg tcgaggaggc cgaccgcctc gtagccgagg tcgaacaggt cggaatagaa
      961 aaacggcagc gtcgtgtagg cctcgtccgc accggccatg acacgcccgg cgtgatgacc
     1021 catgccgatc gcggcgtttt cgtgctccac gcgcagccgc atgtcgagcg cgggattgcg
     1081 gaaattggcg acatcaccgg cagcgaaaat gtccgggtcg gatgtgcgca ggcgatcgtc
     1141 aaccacgatg ccgttgtcga ttttcaaacc ggtctgttca gctagctgca cgttcggagt
     1201 cacgcccaat cccgcgacta gcgcgtcgaa atgcagcttg gtgccatcgt ccagagcaat
     1261 gtcgacagcg tgcgcgtccg ccttgccgcc ggttactttc gccccggcgc gcagttccac
     1321 gccatgctcg cggaaatacg cattaaggaa ttccgatagc cggggcgggt aacgtcccgc
     1381 gccgatggcc tcgccgggaa agatcatggt gaccttgcag ccattcgtgg ccagcgaggc
     1441 ggctaattcg ctgccgatga agccgccgc
//
LOCUS       DASRVO010000205         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_84054, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000205 DASRVO010000000
VERSION     DASRVO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84054"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFF05_02440"
     CDS             complement(<1..1043)
                     /locus_tag="VFF05_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZW50697.1"
                     /translation="MEGRSVEAAFDAGLVTSDAGALLLGATDRAIDLIDRFASCFHDE
                     RRPDLIEHEVATLVGQRVFGIALGYEDLNDHDELRHDPLMAVLAGKLEARREDCAPVA
                     GKSTLNRLELSKLAPTRYHKISHNPMAIRTLLVDLFLEAHARAPKQIILDLDATDDPL
                     HGEQEGRFFHGYYDCYCYLPLYVFCGRHLLAAKLRPANIDGAAGAIEEVARIVAQIRR
                     RWPRVRILLRADSGFAREDLMAWCEANGVDFLFGLAQNTRLIAEIKTELEFVAAKSGR
                     TGKMARRFKSFMWMTRRTWSRRRRIVAKAEWTKGEANPRFVVTSLRRDACKAKHLYEK
                     VYCARGEMENRIK"
     gene            complement(1249..>1507)
                     /locus_tag="VFF05_02445"
     CDS             complement(1249..>1507)
                     /locus_tag="VFF05_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50698.1"
                     /translation="GASMAMAGAFALRQALRDAGAVPAALERYETEQRPVVEAKQASG
                     RRMANWFVPDRRWRLFMRNAALNLTRVPGFDRFLYRSLTAA"
BASE COUNT          266 a          523 c          459 g          259 t
ORIGIN      
        1 tccttgatcc ggttctccat ctcgccgcgc gcgcagtaga ctttctcgta gagatgcttg
       61 gccttgcacg cgtcacgccg cagcgaggtg acgacgaaac gcggattggc ctcccccttc
      121 gtccactcgg ccttggccac gatccggcgc cgccggctcc aggtccggcg cgtcatccac
      181 atgaagctct tgaagcggcg cgccatcttg ccggtacggc cgctcttggc ggcgacaaac
      241 tcgagttcgg tcttgatctc ggcgatgagc cgcgtgttct gcgccagccc gaacaggaag
      301 tcgacgccgt tggcctcgca ccaggccatc agatcctcgc gcgcgaagcc cgagtcggcg
      361 cgcaacagaa tgcgcacccg gggccaccgg cggcggatct gcgccacgat gcgcgccacc
      421 tcctcgatcg cgcccgccgc accgtcgatg ttcgcgggcc gcagcttggc cgccagcaga
      481 tggcgcccgc agaacacgta gagcggcagg tagcagtagc agtcgtaata gccgtggaag
      541 aaccgcccct cctgctcgcc gtgcagcgga tcatcggtcg catcgaggtc gagaatgatc
      601 tgcttcggcg cgcgcgcgtg cgcctccagg aacagatcga ccagcagagt ccggatcgcc
      661 atcggattat ggctgatctt gtggtagcgc gtcggcgcaa gcttgctcag ctccagccgg
      721 ttgagcgtcg acttgccggc caccggcgcg caatcctcgc gccgcgcttc cagcttgccc
      781 gccagcaccg ccatcagcgg atcgtgccgc agttcgtcgt ggtcgttgag atcctcgtag
      841 ccgagcgcga tcccgaacac ccgctggccg accagcgtcg cgacctcgtg ctcgatcaaa
      901 tcaggacgcc gctcgtcgtg aaagcacgac gcaaaccgat caatcaaatc aatcgcccga
      961 tcggtcgctc ccagaagcag cgctcccgca tccgacgtca ccaagccggc gtcaaatgcc
     1021 gcttccacag agcgaccttc cacggttcca aaatcaaatg attctgcgct acactctgtc
     1081 ggcatcgggg gctctccgcg aatctgcgca aagttgttgt ggcacaacca ctttcgctga
     1141 ttcggagccc cgatgcgccc gtttgtgtga gatatccggg ctaacgagag tgcacggagt
     1201 atgggtcccc gttttcgcgg ggacgacaac ggagaggctg tacagaattc acgccgccgt
     1261 cagcgaccga tacagaaacc tgtcgaaccc cggcacgcgc gtcagattga gcgcggcgtt
     1321 gcgcatgaac aggcgccagc gtctgtcggg cacgaaccag ttcgccatcc ggcggccgga
     1381 ggcctgcttg gcttcgacga ccgggcgttg ctccgtttca tagcgttcca gtgcggccgg
     1441 taccgcgccg gcatcgcgca aggcttggcg aagcgcgaag gcgccggcca tcgccatcga
     1501 cgcgccc
//
LOCUS       DASRVO010000206         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_84089, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000206 DASRVO010000000
VERSION     DASRVO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84089"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..641
                     /locus_tag="VFF05_02450"
     CDS             9..641
                     /locus_tag="VFF05_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW50699.1"
                     /translation="MTLSGPAGALEAAVDVPEAADARAGVAIICHPHPQHGGTMHNKV
                     VTMLERSLRESGLATVVFNFRGVGASQGSYDDGRGETQDLIAVAEWVQRTRPDDVLWL
                     AGFSFGSYVAARAAPQLPVRQMISVAPPVSRWDFAHLASPLCPWLVIQGEADEVVDPA
                     AVYAWVAAQPDPPTLVRMPETGHFFHRRLMDLRGAVKNGVRGNLPPLRNA"
     gene            638..1729
                     /gene="zapE"
                     /locus_tag="VFF05_02455"
     CDS             638..1729
                     /gene="zapE"
                     /locus_tag="VFF05_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003469241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HZW50700.1"
                     /translation="MSMARSPSVLYARGVTDKSWDDDPAQRALLPSLDRIRSGLLVAQ
                     SGGWRRWLPRRERAAVHGLYLWGAVGRGKTFLADLLVESLPPHLVLRLHFHRCMGRVQ
                     GQLRSLQGRSDPLREVAARFAAEARLLCLDEFFVNDIGDAMILAGLLEHLFAQGVTLV
                     TTSNIEPAGLYRDGLQRARFLPAIDLLETHCEVRELVSPQDYRLRALTRAGVYFTPTG
                     QHAEHELAACFARIAPGAHRADTAIHINDREIPIRRRGDGAVWFDFAALCEGPRAVAD
                     YIDLAQRFNTVLISNVPHFTPQTEDAARRFVNLVDEFYDRGVKLVLSAATPVTELYGG
                     ERLRSEFARTASRLIEMQSAQYLAAAHRH"
     gene            1921..>2837
                     /locus_tag="VFF05_02460"
     CDS             1921..>2837
                     /locus_tag="VFF05_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW50701.1"
                     /translation="MQMPPPEVGVVVAKAQAVPLTRDLVGRLSATRTADVRARVAGVL
                     QKRAYTEGTDVKQGQLLFQIDPAPLKAALDAQLANLAAAKATYTNNHVAAERARSIAG
                     KGLMSKTDLDNALAAERTAAAAVQQAQANVETARINLGYASVTAPISGRAGQQQVTEG
                     ALVGQGEATLLTTVEQIDPIYVNFSQAVGELDALRRAQAGGHVALDETNKAKVDILLP
                     DGSVYAHSGTLDFSDTAVDAATGAVSLRGSVPNPEHLLLPGMYVKLRLTLGQITQAYL
                     IPQAALQRDPTGPYVLVVGTDGKVARKSVA"
BASE COUNT          454 a          967 c          936 g          480 t
ORIGIN      
        1 caacagccct gacgctctcc ggccccgccg gcgcgctcga agctgccgtc gatgtgcccg
       61 aggccgccga cgctcgggcg ggcgtggcca tcatttgcca tccgcatccg caacatggcg
      121 gcaccatgca caacaaggtg gtgacgatgc tcgagcgcag tctgcgcgaa tcgggcctgg
      181 ccacggtggt gttcaatttc cgcggtgtcg gcgcgtcgca gggaagttac gacgacggcc
      241 gcggcgaaac gcaagacctc atcgccgtgg cggagtgggt gcaacgcacg cgtccggacg
      301 acgtgttgtg gctggcggga ttctccttcg gctcgtatgt ggccgcgcgc gccgcgccgc
      361 aattgccggt acgccagatg atttccgtgg caccgccggt gtcgcgctgg gacttcgcgc
      421 acctggcatc gccgctgtgc ccgtggctgg tgatccaggg cgaggccgac gaggtcgtcg
      481 atccggcggc cgtgtacgcc tgggtggcgg cgcaaccgga tccgccgacg ctggtgcgca
      541 tgcccgagac cggacacttt tttcatcgtc gcctgatgga tctgcgcggc gcggtcaaga
      601 atggcgtgcg cggcaatctg ccgccactgc gcaacgcatg agcatggcgc ggtccccgag
      661 cgtactgtac gcgcgcggtg tgacggacaa aagctgggac gacgatcccg cgcagcgcgc
      721 cctgttgccg agcctggatc gcatccgtag cggattgctc gtcgcgcagt ccggtggctg
      781 gcggcgctgg ctgccgcggc gcgagcgcgc ggcggtgcac ggcctgtatc tgtggggcgc
      841 ggttggacgt ggcaagacgt ttctcgccga tctgctggtg gaatcgctgc caccgcatct
      901 cgtactacgc ctgcatttcc atcgctgcat gggccgcgtg caagggcagt tgaggtcact
      961 gcagggtcga tctgatccgc tgcgcgaggt cgccgcccgc ttcgccgccg aggcgcggct
     1021 gctatgcctc gacgagttct tcgtcaacga catcggcgac gcgatgatcc tcgccggctt
     1081 gctcgaacac ctgttcgcgc aaggcgtgac cctggtgacg acctcgaaca tcgaaccggc
     1141 aggcctgtat cgcgacggtt tgcagcgcgc gcggttcctg cccgcgatcg acttgctcga
     1201 aacgcactgc gaggtgcgcg agctggtttc accgcaggat taccgcttgc gcgcgctgac
     1261 ccgcgccggc gtctacttca cgccgacggg ccaacatgcc gagcacgaac tcgccgcgtg
     1321 cttcgcgcgc atcgcgccgg gcgcgcatcg cgccgacacg gccatccaca tcaacgaccg
     1381 cgaaattccc atccgccgcc gcggcgacgg cgcggtctgg ttcgacttcg ccgcgttgtg
     1441 cgaggggccg cgtgcggtgg cggattacat cgacctggcg caacgcttca acaccgtgct
     1501 gatctccaac gtgccgcact tcacgccgca gaccgaagac gccgcacggc gattcgtcaa
     1561 cctggtcgac gagttctacg atcgcggcgt gaagctggtg ctgtccgcgg ccacgcccgt
     1621 caccgagctg tacggcggcg aacgcctgcg ctcggagttc gcgcgcacgg caagccgcct
     1681 gatcgaaatg cagagcgcgc aatatctcgc cgccgcgcat cggcattgat gcgcttgcgg
     1741 aacctgaatt gtgacctccg gcccattgca catcgcggca tccctcatta tcttgaccga
     1801 ccggttcgct aattgacgcg caacccgacg aaggattcgt ttatgcgatt tcggattact
     1861 cccgccccca tcctgatctt cgcgctcgcc gccgccggct gcggcaaggc gccaccacag
     1921 atgcagatgc cgccgccgga agttggcgtg gtcgtggcca aggcgcaggc cgtgccgttg
     1981 acgcgcgacc tggtcgggcg cctgtccgcc acgcgcaccg ccgacgtgcg cgcgcgtgtg
     2041 gccggggtgc tgcaaaaacg cgcctacacc gaaggcaccg acgtcaagca aggccagctg
     2101 ctgttccaga tcgacccggc accgctcaag gccgcgctcg atgcgcagct tgccaatctt
     2161 gccgcggcca aggcgaccta cacgaacaat catgtcgccg ccgagcgtgc acgttcgatc
     2221 gccggcaagg gcttgatgtc caagaccgat ctcgacaacg cgcttgccgc ggaacgtacc
     2281 gccgcggcag ccgtgcagca agcgcaggcg aatgtcgaaa cggcacgcat caatctgggc
     2341 tatgccagcg tgactgcgcc gatttccggc cgcgcgggcc aacagcaggt gaccgaaggc
     2401 gccttggtcg ggcagggcga agcgaccctg ttgaccacgg tcgagcagat cgatccgatc
     2461 tacgtgaatt tcagccaggc cgtcggcgag ttggatgcgc tgcgccgcgc ccaggccggt
     2521 ggtcatgtcg ccctggacga gaccaacaag gccaaggtcg acatcctgtt gcccgacggc
     2581 tcggtttacg cgcattccgg gacgctggat ttttccgata cggccgtcga tgccgcgacc
     2641 ggcgcggtga gcctgcgcgg cagcgtgccg aatcccgaac atctgttgct gccgggcatg
     2701 tacgtaaaac tgcgcctgac cctgggccag atcacgcagg cgtatctcat tccgcaggcc
     2761 gcgttgcagc gcgaccccac cggaccttac gtgctcgtgg tcggcaccga cggcaaggtg
     2821 gccaggaagt ccgttgc
//
LOCUS       DASRVO010000207         1702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_84207, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000207 DASRVO010000000
VERSION     DASRVO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84207"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VFF05_02465"
     CDS             <1..921
                     /locus_tag="VFF05_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZW50702.1"
                     /translation="ATALAFASGMAAITALLEALPNGARILIPDDCYTGLRLLAQDFL
                     VRRGIVTVAVDMSDLAGVRAASAGGVALIWAETPSNPRMKVADIAALAQIAHAHGALL
                     VCDNTFATPVLQQPLALGADVVMHSTTKYFGGHSDVLGGALVFARRDALHERVAHYRH
                     VTGAIASPFSAWMLLRGCRSLPARMAWHCRNARAVAEFLASHPRVERVNWPGLPTHPQ
                     HEVAAKQMRDFGGMLSVEIRGGREAALAVAAKVRLFTNATSLGGCESLIEHRASVEGE
                     HPLSPQNLLRLSVGLEHADDLVDDLAQALG"
     gene            complement(923..1357)
                     /locus_tag="VFF05_02470"
     CDS             complement(923..1357)
                     /locus_tag="VFF05_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50703.1"
                     /translation="MQYGGVPGCPDRLPGHRACGHLQRRFRCRIVRKFGKCRQYRGTD
                     RGKRRPRFQLHRDDFSISPVYQANYDLIADGNDGKYSAQLLQAPVTIFGYNIENNPDC
                     PFASVFIGGSSVLTDVQDAATTVVEARLRASTLGQAICPLTP"
BASE COUNT          305 a          574 c          530 g          293 t
ORIGIN      
        1 gcaaccgcac tcgcctttgc ctcgggcatg gccgcgatca cggcgctgct cgaagcgttg
       61 ccgaacggcg cgcgcatcct gattcccgac gactgctaca ccggactgcg cctgctcgcg
      121 caggattttc tcgtgcgacg cgggatcgtc accgtcgccg tcgacatgag cgaccttgcc
      181 ggcgtgcgtg cggcgagtgc cggcggcgtg gcgctgatct gggcggaaac gccatcgaat
      241 ccgcgcatga aagtcgcgga catcgcggcg ctcgcgcaaa tcgcgcatgc gcacggcgcg
      301 ttgctcgtct gcgacaacac cttcgccacg ccggtgttgc agcaaccgct cgcgctcggc
      361 gccgatgtcg tcatgcactc gaccacgaaa tatttcggcg gccacagcga cgtgctcgga
      421 ggtgccctcg tgtttgcgcg gcgtgatgcg ttgcacgaac gcgtcgcgca ttaccgccac
      481 gtcaccggcg cgatcgcgtc gccgttcagc gcctggatgc tgctgcgtgg ttgccgctcg
      541 ctgccggcgc gcatggcctg gcattgccgc aacgcgcgcg ctgtcgcgga attcctcgcc
      601 tcgcatccgc gcgtggagcg cgtgaactgg ccgggactgc ccacccatcc gcagcacgaa
      661 gtcgcggcga aacaaatgcg cgatttcggc ggcatgctca gcgtggagat tcgcggtggg
      721 cgcgaagcgg cactcgccgt cgcggcgaag gtaaggctgt tcaccaacgc gacgtcgctc
      781 ggcggctgcg aatcgctgat cgaacatcgc gcctcggtcg aaggcgaaca tccgttgtcg
      841 ccgcagaatc tgttgcgtct atccgtcgga ctcgaacacg ccgacgatct ggtggacgac
      901 ctggcgcaag ccctgggttg aactacggcg tcagcgggca gattgcctgg cccaacgtcg
      961 acgcgcgcag acgcgcctcg acgaccgttg tcgccgcatc ttgcacgtcc gttaggacgg
     1021 aggaaccgcc aataaacacg ctggcaaaag gacaatccgg attgttttcg atgttgtagc
     1081 cgaaaatagt caccggcgct tgcagcaatt gcgcggaata cttgccgtcg ttgccatcgg
     1141 cgatcaggtc gtagttcgcc tgatacacag ggctgatgct gaaatcgtca cggtgcaact
     1201 gaaagcgagg tcgccgcttg ccacgatcgg taccgcgata ttgtcggcac ttcccaaact
     1261 tgcgaacgat ccggcatcga aatcgccgtt gaaggtgccc gcatgcacgg tgaccgggca
     1321 acctgtctgg gcacccgggc acgccgccgt attgcaagcg acgtagctcg tacccccgac
     1381 gctacccgac atcggttcca cgcaagtggt cgccccgtcc agcgaagtgc gcaacagatc
     1441 gagaaaatgc ggctcggtca gcgcctgcgc ccaacacgcc tcgaaaccgt tgtgcgccac
     1501 cagatccgcc gtttgtgcag tcgctgcagc ggacacgacc agcgcgatgg ccacgctgcc
     1561 gaacgaacga tattgaccga acccgggtca cgttgacttg cgcgcacgat tgcccccgca
     1621 tcgaacggat cgcgcatcat agcgcgaccg cgcgcagccg cagcagtgcg ccaccacgca
     1681 cgccgctacc agcttccgtt gt
//
LOCUS       DASRVO010000208         6571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_6244, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000208 DASRVO010000000
VERSION     DASRVO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6244"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFF05_02475"
     CDS             complement(<1..594)
                     /locus_tag="VFF05_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50704.1"
                     /translation="MGEIVDEPGAAAGVVNLPAPMNTGVASQSGRYRPESDSQRRAGG
                     NAGERVQHHVPAANGKTNAAQAESRAHYFERDALRIDLHVRCGKRIGRIQAKCNRMGQ
                     SVENTPHARVVAVDHGPAGRAIGEIGKRGIEAFLAAVMRAVLAVDIGDDRQTRVVAGK
                     IPECLAGLDDEMRAASDPRTFSRERRDVGAQRKARLGA"
     gene            511..5223
                     /locus_tag="VFF05_02480"
     CDS             511..5223
                     /locus_tag="VFF05_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZW50705.1"
                     /translation="MRRYTGIHRRWQVHDTGGGPGLIDDFAHHPTELEALIDTARTTL
                     PGDRRLVIAFQPQLYSRTKALLNDFGRVLARFDAAWVLDIDGAGETDSGEISSADLVA
                     AIRRHDGTAMGVGNAAALLRRIPQCLRSGDTLITAGAGSIAGVVEPIACVLRDMNSAD
                     VVADVPAAPAVRESGRAYRRGLATSTGTVLNLFAMQVDSQPQAPAVNVDGATLSYGQL
                     DAQAEALARRLLALGLALQGVVGVHAPSSIELIVAVLAIAKAGGIYLPVDPGLPAGRM
                     GFMLRDAQARFLLTAGGAPLDQGMDVAVLHFDELMRASAEASGSAASVAIAGEHTAYV
                     CYTSGSTGRPKGVAIPHVCLANFAAAAIGRFAVAPGTRMALNTAIGFDVSLGEIWVTL
                     CAGGELFATGSAKPLIGDALCDFVDRNRITHLAITPTVLSTMPTARLASLRCVIAAGE
                     ACPPALVARWAPGRRFFNAYGPTEATIYASVGECHAQGAVNIGTPLAHVALRILDGDL
                     REVDDGAVGELCIGGAGVAAGYIALPAETASRFIAWHGPVGRIERLYRSGDLVRRNGD
                     GNLCFVGRLDSQVKILGSRVELEEIEQTLLARFPALLDVAVAFDAGRGGNGRLVCFAV
                     TREDEFDWETARGDLEDWLPAYMVPADFVVVERMKITPSGKKDRAGLLAAHPHGQVRR
                     GEYIAPSNALEARIAAIWKTVLDLDTDVGVDERFDWLGGDSLKALQIVMAVEESCDVA
                     VSPGYFGALSTVGRMAIQLADLLWDRASPAPEPSTGFRATRVYKELRNITANWAGVRA
                     DPDALIVSVGAGQPAYQFLLCVQMESEVHGFAAALGDDFRVHGLRSGHLVMQYDPESL
                     DMLCAHYVEEIEQAQLQGKLLVGGICQGALVAIRLARMLRERGHALELVALIEQSKIA
                     PVEGDVAFYFSEDSFANPFRRFACARSRFEAVYGKNYSLDIVPGAHGHIHEAPQVHIL
                     AHKLRARLGAGRAASSLREASGHSPESAPLPPGRRQAKADIAAAKPVPEPHFADIVDA
                     DTKLIEASGFLDPDYYWSQLKDGSPSGLAAAHYGRWGWLAGLDPGPRFSTRSYLERNY
                     DVRDAGVNPLAHYLRFGASEGRGVCAVSQDVEEVRLVQASGFLDPDFYSAQLKGVALT
                     GPAAAHYVRWGWRDGLDPGPHFSTLGYFERYPDVRDGGANPLLHYLRHGMGEGRSAWS
                     EAEVARWQGGINVHRDAAVAGLREQPRFAPRLRRGDRVAIHAHSRGHFAFRQFQRMLA
                     QAFGAIGIQCTLETEASDLVGPAPALRIVVAPHDFFHLHPLPDSRSFADAALFNSEQM
                     PSVWFNRALPRLMAAPLVLDIHFQTAACLAELGARAVYLPLGIVPGNEIFRLLPDLSA
                     EPAVRGLPDDMKRPIAALSNPLAERGIDVLFVGSNSDRRTRYFTEHSAFFAVNRCFIR
                     LVDVIGPLEETHPRGITARAFAGLAQRSKILMNIHHFDAPYFEWQRLVHYGFAQRCCV
                     VTERCSRPPGFVPGVHYLEDDKHALPALMDWLLHDPDGQEKMEKVGRAGYEMARDSLR
                     LDSALRRLFRLDEPADAP"
     gene            5392..5468
                     /locus_tag="VFF05_02485"
     tRNA            5392..5468
                     /locus_tag="VFF05_02485"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5426..5428,aa:Arg,seq:acg)
     gene            5544..5620
                     /locus_tag="VFF05_02490"
     tRNA            5544..5620
                     /locus_tag="VFF05_02490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5578..5580,aa:Pro,seq:tgg)
     gene            5657..5733
                     /locus_tag="VFF05_02495"
     tRNA            5657..5733
                     /locus_tag="VFF05_02495"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5691..5693,aa:Arg,seq:tct)
     gene            5768..5844
                     /locus_tag="VFF05_02500"
     tRNA            5768..5844
                     /locus_tag="VFF05_02500"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5802..5804,aa:His,seq:gtg)
     gene            5860..5935
                     /locus_tag="VFF05_02505"
     tRNA            5860..5935
                     /locus_tag="VFF05_02505"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5893..5895,aa:Lys,seq:ctt)
     gene            5989..6073
                     /locus_tag="VFF05_02510"
     tRNA            5989..6073
                     /locus_tag="VFF05_02510"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6023..6025,aa:Leu,seq:tag)
     gene            6158..>6571
                     /locus_tag="VFF05_02515"
     CDS             6158..>6571
                     /locus_tag="VFF05_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HZW50706.1"
                     /translation="MQVLVENVGKLERKLVVRLPADAYESTVRSRIADAGRNVRLKGF
                     RPGKVPTKVIEQRFGAQIRGEAMSELVRTSFQQAVSEQKLRPAASPAISTSGEPVDGQ
                     IEYTATFEVLPEIGKLDVASLAITKATASVGESDVD"
BASE COUNT         1070 a         2033 c         2202 g         1266 t
ORIGIN      
        1 ggcgccgagc cgagctttgc gctgggcgcc gacatcccgg cgctcgcggg agaacgtgcg
       61 cggatcggac gcggcgcgca tttcgtcgtc gaggcctgcg aggcattcgg gaatcttgcc
      121 agctacaacc cgggtttgtc ggtcatcgcc aatatcgacg gcgagcacag cgcgcattac
      181 ggcggccagg aacgcctcga tgccgcgttt gccgatttcg ccaatcgcgc gcccggcggg
      241 gccgtggtca acggcgacga cccgggcgtg cggcgtattt tcgaccgact gacccatccg
      301 attgcatttg gcttggatcc ggccaatacg ttttccgcag cgcacgtgca gatcgatgcg
      361 cagggcgtcg cgttcgaagt agtgcgcgcg ggactccgcc tgggctgcgt tcgtcttgcc
      421 gttggcggcc ggcacatggt gttgaacgcg ctcgcctgcg ttgccgccgg cacggcgttg
      481 ggaatcggat tcggggcgat agcggccgga ttgcgacgct acaccggtat tcatcggcgc
      541 tggcaggttc acgacaccgg cggcggcccc gggctcatcg acgatttcgc ccatcatccg
      601 acggagttgg aggcgctgat cgatacggcg cgaaccacgc tgccggggga tcgacgcctg
      661 gtgatcgcgt ttcaaccgca actgtattcg cgcaccaagg cgctgttgaa cgactttggc
      721 cgtgtgctgg ccaggttcga cgcggcctgg gtgctggata tcgacggcgc gggagaaacc
      781 gattccggcg agatctcgag cgccgatctc gtcgcggcga ttcgccgcca tgatggcacg
      841 gcgatgggcg tgggaaacgc cgccgcgctg ctgcggcgca taccgcaatg cctgagatcc
      901 ggcgatacgc tgatcacagc gggggccggt tccattgcgg gcgtggtcga acccattgca
      961 tgcgttctgc gcgacatgaa ttccgcggac gtggtggcgg atgtcccggc ggcgccagcc
     1021 gtccgggaat ccgggcgcgc atatcgacgc ggccttgcga cgagcaccgg cacggtgctg
     1081 aatctgttcg ccatgcaggt cgacagccag ccgcaggcgc ctgccgtcaa cgtggacgga
     1141 gcgacgttgt cgtacggcca actcgatgca caggccgagg cgctagcccg gcgcctgttg
     1201 gcgctggggc tggcgttgca gggcgtcgtc ggcgtgcatg caccttcgtc gatcgaactg
     1261 atcgtcgccg ttctggcgat tgccaaagcc ggtggaatct atttgccggt ggatccgggt
     1321 ctgcctgccg ggcgcatggg attcatgctc cgcgacgcgc aggcccgctt tttgctgact
     1381 gcaggcggtg cgccgctgga tcagggcatg gacgtcgccg tgctgcattt cgacgagctc
     1441 atgcgcgcga gcgcggaagc atccggatcg gccgcttcgg tagccatcgc tggcgaacac
     1501 accgcttacg tgtgctacac ctccggctcg acggggcggc ccaagggggt cgccattccc
     1561 catgtttgcc tcgccaattt tgccgcggcc gccatcggca gattcgccgt ggcgcccggc
     1621 acgcgtatgg cgctcaatac cgccatcggc ttcgatgttt cgctcggcga aatctgggtg
     1681 acgctgtgcg ccggcggcga actgttcgcc acggggtcgg ccaaaccgct gatcggcgat
     1741 gcgttgtgcg acttcgtgga tcgcaaccgg attacccact tggcgattac gccaacggtg
     1801 ctttccacca tgccgacggc gcggcttgcg tcgctgcgct gcgtgatcgc cgcgggcgag
     1861 gcctgtccgc cggcactcgt cgcgcgatgg gcgccggggc gacgattttt caacgcctac
     1921 ggtcccacgg aagccaccat ctacgccagc gttggcgagt gccatgcgca gggtgcggtg
     1981 aacatcggaa cgccgctcgc ccatgtcgcc ctgcgcattc tcgacggcga tctgcgcgaa
     2041 gtggacgatg gcgcggtcgg cgagctttgc atcggcggcg cgggcgtcgc cgccggttat
     2101 atcgcgttgc ccgccgagac cgcttccaga ttcatcgcct ggcacgggcc ggtggggcgg
     2161 atcgagcgcc tgtaccgcag cggcgatctc gtgcgccgca atggcgatgg aaacttgtgt
     2221 tttgtcgggc gcttggacag ccaggtgaag atcctcggca gccgcgtcga actggaggag
     2281 atcgagcaga cgctactcgc aagatttccg gccctgctcg acgtcgcggt ggcgttcgac
     2341 gccggccgcg gcggcaacgg ccgcctcgtc tgcttcgcgg tgacgcgcga agacgagttc
     2401 gactgggaga ccgcgcgcgg cgatctggaa gactggttgc cggcatacat ggtccccgcc
     2461 gacttcgtgg tggtcgaacg gatgaaaatc acgccaagcg gcaagaagga tcgcgcgggt
     2521 ttgctggcgg cgcatccgca cggccaggtc cggcgcggcg aatacatcgc accgagcaac
     2581 gccctggaag cgcgcatcgc cgcaatctgg aagactgtcc tcgacctgga cacggacgtt
     2641 ggcgtcgatg aacgcttcga ttggctcggc ggcgactcgc tcaaggccct gcagatcgtc
     2701 atggcggtag aggaatcctg cgatgtcgcg gtgtcgcccg gatacttcgg cgccttgtcg
     2761 acggtcggca ggatggcgat ccagttggcc gacctgctct gggaccgcgc ctcccccgcc
     2821 ccggaaccct cgacgggttt tcgcgcgacg cgcgtttaca aggaactgcg caacatcacc
     2881 gcgaattggg ctggtgtgcg cgccgacccg gatgcgctca tcgtttcggt cggggccggg
     2941 cagccggcat accagttttt gctgtgcgtg cagatggaga gcgaggtgca cggctttgcc
     3001 gctgcgctcg gcgacgactt ccgcgtccat ggcctgcgct cgggccatct ggtcatgcaa
     3061 tacgacccgg aatcattgga catgttgtgt gcgcactacg tcgaggaaat cgagcaggcg
     3121 cagcttcagg gcaagctgct ggtcggcggc atctgccagg gcgcattggt cgccattcgg
     3181 ttggcgcgga tgttgcgcga acgcggccat gcgctggaac tggtggctct gatcgaacag
     3241 tccaagatcg ctccggtgga gggcgacgtc gccttctatt tttccgagga cagcttcgcc
     3301 aacccgttcc ggcggttcgc ctgtgcgcgt tcgcgtttcg aagccgttta cggcaagaac
     3361 tacagcctgg acatcgtgcc cggcgcccac ggacacattc acgaggcgcc ccaagtccac
     3421 atcctggcgc acaagctgcg tgcccgattg ggcgctggcc gtgccgcttc gtcgctgcgc
     3481 gaggcatctg gccacagtcc ggaatccgcg ccgctgccgc ccggccggcg ccaggcgaaa
     3541 gcggatatcg ccgccgcaaa gccggtgccc gagccgcatt tcgcggatat tgtcgatgcg
     3601 gataccaagc tcatcgaagc cagtggtttt ctcgatccgg attactactg gtctcagttg
     3661 aaggacggtt cgccgagcgg cctggcggca gcgcactacg ggcgttgggg ctggttggcc
     3721 gggctcgatc ctggcccgcg cttcagtacg cgcagctatc tcgaacgcaa ttacgacgtt
     3781 cgcgacgccg gagtaaatcc gctcgcgcac tacctgcgtt ttggggcgag cgaaggccgc
     3841 ggcgtgtgcg cggtcagcca ggatgtggag gaggtgaggc tcgtccaggc gagcggtttc
     3901 ctcgacccag acttctactc ggcccagctg aagggcgttg cgttgaccgg cccggcggcg
     3961 gcgcactacg tacgctgggg ctggcgcgat gggctcgatc ccggtccaca tttcagcacg
     4021 cttggctatt tcgagcgcta tcccgacgtc cgcgatggcg gcgcaaaccc gcttttgcac
     4081 tatctgcgtc atgggatggg cgaggggcgc tccgcgtggt cggaagcgga ggtcgcgcgc
     4141 tggcagggcg gaataaacgt gcatcgcgat gccgccgttg ccgggttgcg cgagcaaccg
     4201 cggttcgcgc cgcgtttgcg ccgcggcgat cgcgtcgcca ttcacgcgca ctcgcgcggt
     4261 catttcgcgt tccgtcagtt ccagaggatg ctcgcgcagg ccttcggcgc aattggcatc
     4321 caatgcacgc tggagaccga agccagcgat ctcgttgggc ctgctccggc cctgcgcatc
     4381 gtcgtcgcgc cgcacgattt tttccatctg catccgcttc cggattcacg aagcttcgcc
     4441 gatgccgcgc ttttcaacag cgaacagatg ccgtcggtct ggttcaatcg cgccttgccg
     4501 cgcctgatgg cggcgccgct ggttctggat atccatttcc agacggccgc ttgtcttgcc
     4561 gaattgggtg ccagggccgt atacctgccg ttgggaatcg tgcccgggaa cgaaatcttc
     4621 cggctgctgc cggacctttc cgccgaaccg gccgtgcgcg gcttgcccga cgacatgaag
     4681 cgcccgattg cggccttgtc gaatccattg gccgagcgcg gcatcgacgt gctgttcgtc
     4741 ggctcgaact ccgatcggcg gacacggtat ttcaccgagc acagcgcctt cttcgccgtg
     4801 aaccgctgct tcattcggct cgtcgacgtg attggcccgc tggaggaaac ccatccgcgc
     4861 ggcattacgg cgcgggcgtt tgccgggttg gcgcagcgct ccaagatatt gatgaacatc
     4921 catcatttcg acgcgcctta cttcgaatgg cagcggctgg tgcactacgg attcgcccag
     4981 cgctgctgcg tcgttaccga acgttgcagc cgtccgcccg gcttcgtgcc cggcgtgcat
     5041 tacctcgaag acgacaagca cgccttgccc gcgttgatgg attggcttct gcacgatccg
     5101 gacgggcagg aaaaaatgga gaaagtcggc cgcgccggct acgagatggc cagggactcg
     5161 ctgcgactgg acagcgcgtt gcggcgactg tttcggctcg acgaacctgc ggatgcacct
     5221 tgacggaaaa tcgcatcgcc gggataatcg ccggttccac gcgcccgtag ctcggttggt
     5281 cgagacgcga gcgctacgcg atgccttgaa ttgcatcgcg tgcgagcggt gaggaggcag
     5341 gatgccgact ggacgaggac caaggatggt tggtcgaatt tgcttaacaa tgcgcccgta
     5401 gctcagttgg atagagtacc aggctacgaa cttggtggtc gggagttcga atctctccgg
     5461 gcgcgccata tcagcgaagg gtgcagcggc atccttcagg tttttgcgag gtgattcggt
     5521 ttgacgatgg cggatcgaac gatcggggta tagctcagcc tggtagagcg ctagctttgg
     5581 gagctagatg tcgagagttc gaatccctct accccgacca accgtctgcg tgggggtgcg
     5641 atagacttta acggttgcgc ccgtagctca accggataga gcaccggcct tctaagccgg
     5701 cggttgtggg ttcgagtccc gccgggcgcg ccatcgacaa gcagaggatt aaagtttggc
     5761 ttttgcggtg ggcgtagctc agttggttag agtaccggat tgtgattccg gttgtcgtgg
     5821 gttcgagtcc catcgtccac cccagttttg acaatttgcg ggccgttagc tcagttggta
     5881 gagcaggaga ctcttaatct cttggtccta ggttcgagtc ctagacggcc caccatcttt
     5941 ttcccgcgtg cacgatgcgc gacaatccgc cgcttgtatc cggcccatgc gaaagaggcg
     6001 gaattggtag acgcactgga tttaggttcc agcggggcaa cccgtgagag ttcgagtctc
     6061 tcctttcgca ccatggactt ggattggcgc tttgcagttg tggcatccgc cgagcatcga
     6121 agcgccaata aacgaacgaa gattttgagg taagaacatg caagttttgg tagaaaacgt
     6181 cggcaagctc gaacgcaagc tggtcgtgcg cctcccggcg gacgcctacg aatccacggt
     6241 gcgttcgcgc atcgccgacg cgggccgcaa tgtccgcctg aagggtttcc gccccggcaa
     6301 ggtgccgacc aaggtgatcg agcagcgctt cggcgcgcag atccgcggcg aagccatgtc
     6361 ggaattggtg cggaccagtt tccagcaggc ggtgagcgaa cagaagctgc gcccggcggc
     6421 gtcgccggcg atttccacca gcggcgagcc agtcgatgga caaatcgaat acaccgccac
     6481 gttcgaagtc ttgccggaaa tcggcaagct tgacgtcgct tcgctggcca tcaccaaggc
     6541 gacggcgagc gtgggcgaat ccgacgtcga c
//
LOCUS       DASRVO010000209         1336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_110050, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000209 DASRVO010000000
VERSION     DASRVO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110050"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFF05_02520"
     CDS             <1..217
                     /locus_tag="VFF05_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50707.1"
                     /translation="HNLIRCRRSRFDDAPTPAEVAAAAASGAHMAHLVMGNPLDPLND
                     VGQQQQEDSAGEHAARKREIEERACDY"
     gene            complement(222..>1336)
                     /locus_tag="VFF05_02525"
     CDS             complement(222..>1336)
                     /locus_tag="VFF05_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZW50708.1"
                     /translation="KVVLVAGLHDPAGASHLLHTITGVPREAIEIRLPRAGGDLGRRL
                     HNDFVVEAALVAKEAGKPVKLVWLREDDLRHDFYRPFGVHDLTATLDRKKRLTGWSHR
                     VAATPRNWRDSRLAGKPIFAGLLDADAFPAGLLPNLQQLFHALPCGLPRGELRGGAHA
                     FQAFAAQCFLDEIAAATHQDPVVLRMDLLGAPRMLRPGFDTGRLAGVLRIAAGKIDWQ
                     HKPKNGHGLGIACHAGDGAYAAHAFEVSVNGNDLVIHRAVCAVDAGRVLNPLNLEAQA
                     MQTTLAAVSATLHEAITLKNGQVQQHNLTDYPLLRMGQAPRGVDVYSVDTGADPANLD
                     DIVAASAAPALANAVYAATTVRVRRLPLMPELLRLL"
BASE COUNT          221 a          429 c          479 g          207 t
ORIGIN      
        1 ccataacctc atccgctgcc ggcgcagccg gtttgacgac gcgccgacgc ctgccgaggt
       61 ggccgctgct gctgcgagcg gagcgcacat ggctcacctg gtcatgggca atccgctcga
      121 tccactgaac gacgtgggtc agcaacaaca ggaagacagc gccggcgagc acgcggcgag
      181 aaagcgcgag atcgaggaac gggcctgcga ctactgagta ttcacaatag acgcaacagc
      241 tcgggcatca gcggcagcct gcgcacgcgc acggtggtcg cggcatagac cgcgttggcc
      301 agcgccggcg ccgcgctggc ggcgacgatg tcgtcgaggt tggcgggatc ggcgccggta
      361 tcgacgctgt atacgtctac accgcgcggc gcctggccca tgcgcagcag cgggtaatcc
      421 gtgagattgt gctgctgcac ctggccgttc ttgagcgtga tcgcctcgtg cagggtggct
      481 gaaaccgcgg cgagcgtcgt ctgcatcgcc tgcgcttcga ggttcaatgg attgagtacg
      541 cggccggcgt cgaccgcgca aacggcgcga tggatgacca gatcgttgcc gttgaccgat
      601 acctcgaacg cgtgcgcggc gtaagcgcca tcgccggcgt ggcaggcgat gccgaggccg
      661 tgcccgttct tcggcttgtg ctgccagtcg atcttgccgg cagcgatgcg caacacgccg
      721 gccaggcgcc cggtgtcgaa tcccggacgc agcatgcgcg gcgcgccgag caggtccatg
      781 cgcagtacga ccgggtcctg atgcgtggcg gccgcgatct cgtcgaggaa acattgtgcg
      841 gcgaaggcct ggaacgcatg cgcgccaccg cgcaattcgc cgcgcggcag gccgcagggc
      901 agggcgtgga acagttgctg gaggttgggc agcaggccgg cgggaaacgc gtccgcgtcg
      961 agcagtccgg cgaaaatcgg ttttccagcc agtcggctgt cgcgccagtt gcgcggcgtc
     1021 gcggcgacgc gatgcgacca gccggtgagg cgctttttgc gatccagtgt cgcggtcaag
     1081 tcgtgcacgc caaagggccg gtagaaatcg tggcgcagat cgtcctcgcg cagccacacg
     1141 agcttcaccg gctttccggc ctctttggcg acgagcgcgg cctcgacgac gaaatcgttg
     1201 tgcaggcgcc tgcccaggtc gccgcccgcg cgcggcaggc ggatctcgat ggcctcgcgc
     1261 ggcacgccgg tgatggtgtg caacaggtgc gaggcaccgg ccggatcgtg caagccggcg
     1321 accagcacga ctttgt
//
LOCUS       DASRVO010000210         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_265958, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000210 DASRVO010000000
VERSION     DASRVO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265958"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFF05_02530"
     CDS             complement(<1..932)
                     /locus_tag="VFF05_02530"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HZW50709.1"
                     /translation="MRISRAQILAPALLLIAAGAATHTNEHGLDPRNFDARTPACTDF
                     FQHADGGWLASNPIPAEYSTWSLDNEIGERNTAILKRILEDAAAHPGAPGSTGQKIGD
                     FYAAAMDEAAIAKASTAPLQNDLTAIAALKSPADVVKLIAAWQARGHDVLFDFEAQPD
                     LKHSTTDIAYAGQGGLGLPDRDYYLRDDAKSKQLRSQYRKHVERMLRLLGDANAKDES
                     DWVMALETRFAKASLDRVALRDPSNSYHIVSLVDADAKTPHFSWTALFHATGRDDVKT
                     FSLAQPEFFTAADAALAEIPIAHWQAWLRWRLVDA"
     gene            complement(995..>1547)
                     /locus_tag="VFF05_02535"
     CDS             complement(995..>1547)
                     /locus_tag="VFF05_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZW50710.1"
                     /translation="PVVAGLFTGISAEFPGAAGWLNQEWFQSIIAFVATVIAFYVVGF
                     ATSRVFGQRLLDAFENLISRIPMVHTIYGGAKKLMTMLSTKPAGTQRVVLIDFPSPEL
                     KSIGFVTRVFTDSAGREVAAVYVPTTPNPTGGYLEIVPMGKLVATDWSMDQAMAFILS
                     GGAVGPDKLPDGAHPETTSPRGQ"
BASE COUNT          268 a          454 c          544 g          281 t
ORIGIN      
        1 gcatcgacga ggcgccagcg cagccaggcc tgccagtgcg cgatcggaat ttccgcgagt
       61 gcggcgtcgg cagccgtgaa gaattcgggc tgggccagcg aaaaggtttt cacgtcgtcg
      121 cggccggtcg catggaaaag cgccgtccac gaaaaatgcg gcgtcttggc gtcggcgtcg
      181 accagcgaca cgatgtggta ggaattggaa ggatcgcgca gggcgacgcg gtcgagcgac
      241 gcttttgcga agcgggtttc aagcgccatt acccagtcgg attcgtcttt cgcattcgca
      301 tcgccgagca gacgcaacat gcgttcgacg tgcttgcggt attggctgcg cagctgcttg
      361 gacttcgcgt cgtcgcgcag gtagtagtcg cgatcgggca gaccgagccc gccttggccg
      421 gcataggcga tgtcggtggt ggaatgcttg aggtcgggct gggcctcgaa atcgaacagc
      481 acgtcgtgtc cgcgcgcttg ccaggcggca atcagcttga ccacgtccgc cggcgatttc
      541 agcgcggcaa tggcggtcag gtcgttttgc agcggtgcag tgctggcctt ggcgatggcg
      601 gcctcgtcca tcgcggcggc gtaaaagtcg ccgattttct gccccgtgct gccgggcgcg
      661 ccgggatgtg cagcggcgtc ctcgagaatg cgtttgagga tggccgtgtt gcgctcgccg
      721 atttcgttgt ccaggctcca ggtgctgtat tcggccggaa tcgggttgga cgcgagccac
      781 ccaccgtcgg cgtgctggaa gaaatccgtg cacgccggcg tacgcgcatc gaagttgcgc
      841 ggatcgaggc cgtgttcgtt ggtgtgtgtc gccgcgcccg ccgcgatcaa aagcagggcc
      901 ggcgccaaga tctgcgcgcg cgaaatacgc atggaaactc ccaatggggg aggcgagtgt
      961 aggggctaag ccgccgccgg cgatgtgtcg aaggtcattg cccgcgcggc gatgtggtct
     1021 ccggatgcgc cccatccggc agcttgtccg gcccgaccgc gccgccggac aggatgaagg
     1081 ccatcgcctg atccatgctc cagtcggtgg cgacgagttt tcccatcggc acgatttcga
     1141 ggtagccgcc ggtgggattg ggcgtggttg gcacgtagac cgccgcgacc tcgcgcccgg
     1201 cgctgtcggt gaacacgcgg gtgacaaagc cgatcgattt caattccggc gagggaaagt
     1261 cgatcagcac cacgcgctgc gtgccggccg gcttggtcga gagcatggtc atcaattttt
     1321 tcgcgccgcc gtagatcgtg tgcaccatcg ggatgcgcga gatcaggttt tcgaaggcat
     1381 cgagcagacg ctggccgaac acgcgtgagg tggcgaatcc aacgacgtag aacgcgatca
     1441 cggtggcgac gaaagcgatg atggactgga accattcctg gttcagccag cctgcggcgc
     1501 cgggaaactc cgcggagatg ccagtgaaca atccggccac gaccggc
//
LOCUS       DASRVO010000211         4349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_161937, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000211 DASRVO010000000
VERSION     DASRVO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161937"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFF05_02540"
     CDS             complement(<1..322)
                     /locus_tag="VFF05_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50711.1"
                     /translation="MVAMTIYVIVFLWVWPLARAADTLWLKAAYALAPVPPLLYAIWL
                     LARRVLSADELEQRTHLIGIGVAAAAISVFGIVSGFLAVAHVFSPEWAAAALVWIFPL
                     LMLCY"
     gene            complement(452..1192)
                     /locus_tag="VFF05_02545"
     CDS             complement(452..1192)
                     /locus_tag="VFF05_02545"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HZW50712.1"
                     /translation="MADHFQLRLAESRMLAPAIRHMAFERVDGAPFAFVPGQFVQIHF
                     NYADGKPTKRSYSVATIGDGGAVRRIEIAVSYVEGGAATDLLANLKEGETIAASGPYG
                     RFCLDDKDANRRYLLVATGTGVTPYRAMLPQLEKLGARGAEVALVYGARNEAELLYGE
                     EFEAFAKKNPHFHFYACFSRAARVQPRAHDRGGRVHVALAELKPDAAHDIAYLCGNPD
                     MVDQAFALLKEAGLPVPHIRREKYVSSR"
     gene            1254..2189
                     /locus_tag="VFF05_02550"
     CDS             1254..2189
                     /locus_tag="VFF05_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZW50713.1"
                     /translation="MSAIPALEIQDLKKTYANGVEALKGVSLAVQPGDFYALLGPNGA
                     GKSTLIGIVSSLVNATSGDARVFGVSIRERRSEAMQMLGLVPQEINFNMFEQPFQICL
                     NQAGFYGVPRAEAKLRAEKYLKQLALWDKAFEVSRTLSGGMKRRLLIARAMMNEPKLL
                     ILDEPTAGVDIEIRRSMWKFIQAINAAGTTVILTTHYLEEAESLCRNIAIIDHGRIIE
                     NTAMKTLLSKLDVEAFLLDLAKPCTELPAVDGVKLTRVDAQTLEAEMSRSHDLNSLFA
                     ALTARGLTVTSMRNKANRLEELFVRLIDANDKEAA"
     gene            2186..2977
                     /locus_tag="VFF05_02555"
     CDS             2186..2977
                     /locus_tag="VFF05_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW50714.1"
                     /translation="MNAELTLIALGTIVRREVMRILRIWTQTLLPPAITVTLYFIIFG
                     ALIGSRIGEMARTDAGAPISYIEYIAPGLIMMSVIQNAYGNITSSFFGAKFQRFVEEM
                     LVSPMPSWTILTGYISGAVLRGFMVGVIVLGISLFFTKIHLYHPFVTLSTFVLAATIF
                     GLLGFVNAIFARKFDDIAIIPTFILTPLTYLGGVFYNVAQLPSPWKEISHLNPILYMV
                     GAFRYGLLGVNEINLVWAYAVMLAFVVALGAFCLTLLNRGVGLRS"
     gene            complement(2974..3771)
                     /locus_tag="VFF05_02560"
     CDS             complement(2974..3771)
                     /locus_tag="VFF05_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZW50715.1"
                     /translation="MQLVDIGANLTHESFRHDFADVLARARAAGVIQLVVTGASLQGS
                     RDALDLARAHPSVLFATAGVHPHHASDFNTETETGLRTLHALPEVVAVGETGLDFHRN
                     FSPREAQLFAFERQLELAAECGKPLFLHQRDAHADFIACMDNVLDQSGRVGRVVVHCF
                     TGEKDELFDYLDRDFYIGITGWICDERRGTHLRELVKSIPANRLMLETDAPYLLPRTV
                     KPQPAHRRNEPMYLAHVCAEVARDRGEDVTVTAANATAAAKEFFGLP"
     gene            3890..>4349
                     /locus_tag="VFF05_02565"
     CDS             3890..>4349
                     /locus_tag="VFF05_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50716.1"
                     /translation="MNRSTIISCAVATAALAVAALAQAATGAASAVSQVDLARAQQVR
                     AQMQAQTNQAKTAAQKSTVAGAKPGRATASELYANPYRAYPPSCLNSPMPLGMWQNDP
                     NAITKQTYLIGDPYSGGAEAGYLERVTIYLFRVACSSGKSATLLEIDRPSN"
BASE COUNT          833 a         1404 c         1325 g          787 t
ORIGIN      
        1 cgtagcacag catcagcagc ggaaatatcc agaccagcgc cgctgccgcc cactctggtg
       61 aaaacacgtg cgcgactgcc agaaatccac tgacgatgcc gaaaacggat atcgcggccg
      121 cggccacacc aataccgatc aggtgcgtgc gctgctccaa ttcatcggcc gacaggacac
      181 gtcgtgccag cagccagatc gcgtacagca gtggcggcac cggtgcgagc gcgtaagccg
      241 ccttcagcca cagcgtgtca gccgcgcgcg ccaacggcca tacccacaaa aacacaatga
      301 catagatcgt catggcgacc atgacgcggc gctgatgact ttgtcgactg atgcggacca
      361 tcgtatccat gccctcgcaa tgtcaagttt ccttgacagc acgaactgta gcggtcgatg
      421 cggacgctgt caagcatcct ttacagcgcg attatcgcga cgagacgtat ttctcgcgcc
      481 ggatgtgcgg caccggcagc ccggcttcct tcaacaatgc gaacgcctgg tcgaccatgt
      541 ccggattgcc gcacaggtag gcgatgtcgt gggcggcgtc gggtttcaat tcagccagcg
      601 cgacgtgcac gcgaccgccg cggtcgtgcg cgcgcggctg tacgcgcgca gcgcgactga
      661 agcaggcata gaagtggaaa tgtggattct tcttcgcgaa cgcttcgaac tcctcgccgt
      721 agagcaactc agcctcgttg cgcgcgccat agacgagtgc gacttcggcg ccgcgcgcgc
      781 cgagtttttc cagttgaggc agcatcgcac ggtaaggcgt caccccggtg ccggtggcga
      841 ccagcaggta acggcgattg gcatccttgt cgtcgaggca gaagcggccg tacggaccgc
      901 tcgcggcaat cgtctcgcct tccttcagat tcgcgagcag gtccgtcgcg gcaccgccct
      961 cgacgtagga gaccgcgatc tcgatgcgcc gaacggcgcc gccatcgccg atcgttgcca
     1021 ccgagtagct gcgcttggtc ggcttgccgt ccgcataatt gaaatggatc tgcacgaact
     1081 ggccgggcac gaacgcgaac ggcgcaccgt cgacacgctc gaacgccatg tggcgtatcg
     1141 ctggcgcgag catgcggctt tcggcaaggc ggagctggaa atggtcggcc acggcagaaa
     1201 tcggcgaatc gaaagggccg ctatactacc cgtcccccac cgattatgca gccatgagcg
     1261 ccattcccgc gctggaaatc caggacctga agaaaaccta cgcgaacggc gtcgaggcgc
     1321 tcaagggcgt ttcgctcgcc gtgcaacccg gcgatttcta tgccctgctc ggcccgaacg
     1381 gtgccggcaa gtcgaccctg atcggcatcg tctcgtcgct ggtcaatgcc acctccggcg
     1441 atgcacgcgt gttcggcgtg tcgatccgcg agcggcgcag cgaagccatg cagatgctcg
     1501 gcctggtgcc gcaggaaatc aatttcaaca tgttcgagca gccgttccag atctgcctga
     1561 accaggcggg tttctacggc gtgccgcgtg cggaagcgaa attgcgtgcg gaaaaatacc
     1621 tcaagcaact cgcactctgg gacaaggcgt tcgaggtgtc gcgcacgctg tccggcggca
     1681 tgaagcgacg cctgctgatc gcgcgcgcga tgatgaacga accgaagctg ctgatcctgg
     1741 acgaacccac cgccggcgtc gacatcgaga tccgccgctc gatgtggaag ttcatccagg
     1801 ccatcaacgc cgccggcacc acggtcatcc tgaccacgca ttacctcgag gaagccgagt
     1861 cgctgtgccg caacatcgcc atcatcgacc acggccgcat catcgagaac acggcgatga
     1921 agacgctgct gtccaagctc gacgtggaag cgttcctgct cgacctggcc aaaccctgca
     1981 ccgagcttcc ggccgtggat ggcgtgaagc tcacgcgcgt cgatgcacag acgctggaag
     2041 cggaaatgtc acgctcgcac gatctcaatt ccctgttcgc ggcgctcacc gcgcgcggcc
     2101 tgaccgtgac ctcgatgcgc aacaaggcga accggctgga agaattgttc gtgcgcctga
     2161 tcgatgcgaa cgacaaggag gcggcatgaa cgccgaactg acgctgatcg cgctcggcac
     2221 catcgtgcgc cgcgaggtga tgcgcatcct gcgcatctgg acgcaaaccc tgctgccgcc
     2281 ggcgatcacg gtgacgttgt acttcatcat cttcggcgcg ctgatcggca gccgcatcgg
     2341 cgagatggcg cgcacggatg ccggcgcacc gatttcctac atcgaataca tcgcgcccgg
     2401 gctgatcatg atgagcgtga tccagaacgc ctacggcaac atcaccagtt ccttcttcgg
     2461 cgcgaaattc cagcgcttcg tcgaggaaat gctggtttcg ccgatgccaa gctggacgat
     2521 tttgaccggc tacatttccg gcgccgtgct gcgcggcttt atggtcggcg tgatcgtgct
     2581 cggcatatcc ctgttcttca ccaagatcca cctctaccat ccgttcgtca cgctcagcac
     2641 gttcgtgctg gccgcgacga tcttcggtct gctcggtttc gtcaacgcga tcttcgcgcg
     2701 caagttcgat gacatcgcga tcatcccgac cttcatcctg acgccgctca cctaccttgg
     2761 cggcgtgttc tacaacgtcg cgcagttgcc gagtccgtgg aaggaaatct cgcacctgaa
     2821 cccgatcctg tacatggtcg gcgcgttccg ctacggcctg ctcggcgtca acgaaatcaa
     2881 cctggtttgg gcctacgcag tgatgctggc attcgtggtc gcgctcggcg cgttttgcct
     2941 gacacttctc aatcggggcg tcgggctgcg gtcctagggc agtccgaaaa actccttggc
     3001 tgccgccgtc gcgttcgcgg cggtgacggt cacatcctcg ccgcggtcgc gtgcgacttc
     3061 cgcgcagaca tgtgcgagat acatcggctc gttgcgccga tgcgccggct gtggtttcac
     3121 cgttcgcggc aacaggtagg gcgcgtcggt ttccagcatc agacggttcg cgggaatgga
     3181 tttgaccagc tcgcgcaggt gcgtgccgcg gcgctcgtcg cagatccagc cggtgatgcc
     3241 gatataaaaa tcgcgatcga ggtaatcgaa caattcgtcc ttctcgccgg tgaagcaatg
     3301 aaccacgacg cgtccgacgc gcccgctctg atcaagcacg ttgtccatgc aggcgatgaa
     3361 atccgcatgc gcgtcgcgct ggtgcaggaa cagcggcttg ccgcattcgg cagcgagctc
     3421 gagctggcgc tcgaacgcga acaactgcgc ctcgcgcggc gaaaaattgc gatggaagtc
     3481 caggccggtt tcgccgaccg cgacgacttc gggcaacgca tgcagcgtgc gcaagccggt
     3541 ctcggtttcc gtattgaagt cgctcgcatg atgcggatgc acgcccgcgg tcgcgaacag
     3601 cacgctcgga tgcgcgcgcg ccaggtccag cgcatcgcgc gagccttgca gggatgcgcc
     3661 ggtcacgacg agctggatca cgcccgccgc acgtgcgcgc gcaagcacgt ccgcgaaatc
     3721 gtggcggaag gactcgtggg tcaggttggc gccaatgtcg acaagttgca tggccgcgcg
     3781 ttatcgggaa agtctggcat tatgccaaca cgatgccggc tgcgttcacg gccggttaac
     3841 tggtgccgcc acaggctgcg ccacggggtt tcgatttggg agtatcgcta tgaatcgttc
     3901 gaccatcatt tcctgcgccg ttgcgactgc cgcactggcc gtggccgcgc tcgcacaagc
     3961 ggcaaccggc gccgcatccg cggtgtcgca agtggatctg gcgcgtgcgc aacaggtgcg
     4021 tgcgcagatg caggcacaaa ccaaccaggc aaagaccgct gcgcaaaaat ccactgtcgc
     4081 cggcgccaaa cccggccgcg ccacggcttc ggagttgtac gcgaatccct atcgcgccta
     4141 tccgccgagc tgcctgaact cgccgatgcc gctgggcatg tggcagaacg acccgaatgc
     4201 gatcacgaag cagacctacc tgatcggcga tccctattcg ggcggtgccg aagccggcta
     4261 ccttgaaagg gtcacgatct atctgttccg cgtcgcatgc agcagcggga aatcggcaac
     4321 gttgctggag attgaccgtc cctccaatg
//
LOCUS       DASRVO010000212         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_188641, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000212 DASRVO010000000
VERSION     DASRVO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188641"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..>2572)
                     /locus_tag="VFF05_02570"
     CDS             complement(91..>2572)
                     /locus_tag="VFF05_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZW50717.1"
                     /translation="GARSAFAQALFAAYPFALVDEFQDTDKQQFEIFDAIYRDARHTP
                     RGWLAMIGDPKQAIYGFRGGDIAAYLDARESATTRRAMDTNFRSTQVLVDVLNALYGA
                     SDGGFGDARIVYQPMRCGGRAEDAALRRKSDPVAPLYFHLFRGTALGKTGELENTLAR
                     LDELALDDCVERIVELLNDSTTTLGNNRVVPQDIAVLVPNNRHVVSLRQSLAARGVPC
                     VGSGRDSVFDGETAEELELFLAAMLHADDDGAVCGALATTLLGYDWRGILGMRDDPAA
                     FERELERFAQWQALARSRGVFGAVADLIAQRAGVLMRYDEGERILADLRHLGELIAAR
                     EAEESGIEGVIAWFARTRRDGANDGERDYRVRKASDAGAVRIMTLHAAKGLEFPVVFL
                     PLAWRVAKRDGQHEPKVPRFHDAQGRLCIDAGSGEFADNLELHFAEDLRERQRLLYVA
                     MTRAQSALHVYWADRGVPADAAAWQIPAIDILLRQVMEKSSLAFGEADLPALVRKLGG
                     MAAIDAAARNDAKLVAADVVRADFTPRSPLPALRPFEWLHSFTGITRRRIAEDADAAA
                     ADESEVALPDIETVATQAEHPELLALDAFRGRRFGDAVHNILEESGMRPVERSVLLAG
                     LAAEGLDAEHPEAESALLQMLERTRESDLGGGLRLMDLPAHERVAEFGFEFPIAASLQ
                     ELRTACAAHGFADIWPAHLRTHELRGMLTGFADLIFVFDGRYNVLDYKTNRLGTRLSD
                     YAGAALDAAMSEHFYPLQALLYTVALHRYLRGRLRDYSPERHLGDSVYLFLRGVGLAP
                     AAGVWRRRWPAKLILDLDDVFAGAEVAA"
BASE COUNT          466 a          870 c          822 g          414 t
ORIGIN      
        1 ccgcgagtgc gagcaggtcc gaaccgctgc gctggcgcac ccagcgcgcc agggcctggt
       61 cgaaatcgcg ttcgggaagc acggtcgcgt tcatgccgcc acctccgcac ccgcgaaaac
      121 atcgtccaga tcaagaatca atttcgccgg ccagcgccgc cgccatacgc ccgcagcggg
      181 cgcgagtccg acgccgcgca ggaacaggta cacgctgtcg cccagatgcc gctccggcga
      241 atagtcgcgc aggcgtccgc gcaggtagcg atgcagcgcc accgtataca gcaaggcctg
      301 caagggataa aaatgttcgg acatcgccgc gtcgagcgcg gcgccggcgt agtcggacag
      361 gcgcgtgccg agccggttgg tcttgtagtc gagcacgttg tagcggccgt cgaagacgaa
      421 gatcaagtcg gcaaagccgg tcaacatgcc gcgcagttcg tgcgtgcgca gatgcgccgg
      481 ccagatatcg gcgaatccgt gcgccgcgca ggctgtgcgc agttcttgca acgacgccgc
      541 gatcggaaac tcgaatccga attccgccac gcgctcgtgc gcgggcaaat ccatcaggcg
      601 caagccgcca cccagatcgg attcgcgcgt gcgttcgagc atttgcaaca acgcggattc
      661 ggcttcggga tgctcggcat cgaggccttc cgccgccaat ccggcgagca gcacggagcg
      721 ctcgaccggg cgcatgccgg attcttcaag gatgttgtgc acggcgtcgc cgaatcggcg
      781 tccgcgaaag gcatccagcg ccagcaattc cggatgctcg gcttgcgtcg caaccgtttc
      841 gatgtcgggc agcgcgactt cggattcgtc ggccgccgct gcgtcggcgt cttcggcgat
      901 gcgtcggcgc gtgatgccgg tgaaactgtg cagccactcg aacggacgca gcgcgggcaa
      961 cggcgaacgc ggcgtgaaat ccgcgcgcac aacatccgcc gcgacgagtt tcgcgtcgtt
     1021 gcgcgccgcc gcatcgatgg cggccatgcc gccgagtttg cgcacgagcg ccggcaaatc
     1081 cgcttcgccg aacgcgagtg aggatttctc catcacctgc cgcaacaaga tgtcgatggc
     1141 cggaatttgc cacgccgccg catccgccgg cacgccgcga tccgcccagt acacgtgcaa
     1201 tgcggattga gcgcgcgtca ttgccacgta cagcaggcgc tggcgctcgc gcaaatcctc
     1261 ggcgaagtga agttcgagat tgtcggcgaa ttcgcccgag ccggcgtcga tgcacagccg
     1321 gccttgcgca tcgtgaaagc gcgggacttt cggttcgtgt tgaccgtcgc gcttggcgac
     1381 acgccaggcc agcggcagga agacgacggg gaattccaga cccttggccg catgcagggt
     1441 catgatccgc accgcgcccg catccgaggc cttgcgcacg cgatagtcgc gctcgccgtc
     1501 gtttgcccca tcgcgacgcg tgcgcgcgaa ccaggcaatc acgccctcga tgccggattc
     1561 ttccgcttcg cgcgcggcga tcagttcgcc aagatgacgc aagtcggcga ggatgcgctc
     1621 gccttcatcg taccgcatca gcactcccgc acgctgcgcg atcagatccg cgaccgcgcc
     1681 gaacacgcca cgcgagcgcg ccaacgcctg ccattgcgca aaacgctcca gctcgcgttc
     1741 gaacgcggcc ggatcgtccc tcatgccgag gatgccgcgc cagtcatagc cgagcagggt
     1801 cgtggccagc gcaccgcaca cggcgccgtc gtcgtccgca tgcagcatgg cggcgaggaa
     1861 caattccagt tcttcggcgg tttcgccatc gaacacgctg tcgcgtccgc tgcccacgca
     1921 cggcacgccg cgtgccgcga gcgactggcg caaggacacc acatgccgat tgttcggcac
     1981 cagcacggcg atgtcttgcg gcacgacgcg attgttgccg agcgtggtcg tcgaatcatt
     2041 gagcaattcg acgatgcgtt cgacgcagtc gtcgagtgcg agttcgtcca gacgggccag
     2101 tgtgttctcc aactctcctg tcttgccgag cgccgtgccg cgaaacaaat gaaaatacaa
     2161 cggcgcgacg gggtcgctct tgcggcgcaa tgccgcgtct tccgccctgc cgccgcagcg
     2221 catgggctga taaacgatgc gcgcatcgcc aaagccaccg tcgctggcgc cgtacagagc
     2281 attcagcaca tcgaccagca cctgcgtgga acggaaattc gtgtccatcg cgcggcgcgt
     2341 ggtcgcgctt tcgcgcgcat caaggtaggc cgcgatgtcg ccgccgcgaa aaccatagat
     2401 cgcctgtttc ggatcgccga tcatggccag ccagccgcgc ggcgtgtgac gcgcatcgcg
     2461 gtagatggcg tcgaagatct cgaactgctg cttgtcggtg tcctggaatt catcgaccag
     2521 cgcgaacgga taggccgcga acaaggcttg ggcgaatgcg ctgcgcgctc cg
//
LOCUS       DASRVO010000213         1799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_6466, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000213 DASRVO010000000
VERSION     DASRVO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6466"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1799)
                     /locus_tag="VFF05_02575"
     CDS             complement(<1..>1799)
                     /locus_tag="VFF05_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW50718.1"
                     /translation="DHRGRIVYCNECFARLTGWTVMELSGQDYFKLFVAESDRQQRRQ
                     RFEERLAEANANRHHEGEIVIRSGERRMLRWSHTLLRDGDAPIGTASLAEDVTEQRAA
                     QARVAFLANHNAASGLPRIGIITEFLEAACIQALQQDSRVVVLYINLDRFHALNEMRG
                     RAAGDRVLATVGRRLVDVVGEMGIVGHVASDEFVLVLKDPTRIQDQAEFGESVRARVE
                     EPIDTDLYISCSIGVSCLPDNAASADELLHQAESAMRRAKTEGRNTVFAFASEHQQEL
                     KDRLALGVRLKEAVRAGEFTLHYQPRINGRDWRVGGFEALLRWHHPDFGLLLPSRFLG
                     VAEDLGLMVEIGSFVLQSACRQARAWLDSDARDFSISVNVSPLQMQRPGFVDEVRDVL
                     ARFKLPAQCIELELTETMMMGNVERVIGTMRALKALGVKLSLDDFGTGFSSLNYLRRF
                     PLDTLKIDQSFVRDISTDPGAAGVCRAIITLGHQLNMTVLAEGVEIDAQIGYLRRNEC
                     DDFQGYYFGKPVAPDRALEMLQHRYLAHERIDHAAEEKPTLLLVDDEENILNALTRML
                     RRDGYRILTATNADDALDIVGRNDVQVVLSDQR"
BASE COUNT          288 a          557 c          628 g          326 t
ORIGIN      
        1 gcgctggtcg gagaggacga cctgcacgtc gttgcggccg acgatgtcga gcgcgtcgtc
       61 ggcgttggtc gcggtcagga tgcggtagcc gtcgcggcgc agcatgcgcg tcagcgcgtt
      121 caggatgttc tcctcgtcgt cgacgagcag cagggtcggc ttttcctccg ccgcatggtc
      181 gatgcgctcg tgcgcgagat agcggtgctg cagcatttcc agtgcccggt cgggcgcgac
      241 cggcttgccg aagtagtaac cttggaaatc gtcgcattcg ttgcgccgca gatagccgat
      301 ctgcgcgtcg atctcgacgc cttcggcgag cacggtcatg ttgagctggt ggccgagcgt
      361 aatgatcgcc cggcacacgc cggcggcgcc gggatcggtg ctgatgtcgc ggacgaagct
      421 ctggtcgatc ttcagcgtgt cgagcgggaa gcggcgcagg tagttcaggc tggagaatcc
      481 ggtgccgaag tcgtcgagcg acagtttcac gccgagcgcc ttgagcgcgc gcatcgtgcc
      541 gatgacgcgc tccacgttgc ccatcatcat cgtctcggtc agttcgagct cgatgcattg
      601 cgccggcaac ttgaaccttg ccagcacgtc gcgcacttcg tcgacgaacc cgggacgctg
      661 catctgcagc ggcgatacgt tgaccgaaat cgagaagtcg cgcgcgtcgg aatccagcca
      721 ggcgcgagcc tgacggcagg cgctttgcag cacgaagctg ccgatttcaa ccatcaggcc
      781 gagatcctcg gcaacgccga ggaaccgtga cggcaggagc aggccgaaat ccggatgatg
      841 ccagcgcagc aacgcctcga acccgccgac gcgccagtcg cggccattga tgcggggttg
      901 atagtggagc gtgaactcgc cggcgcgtac cgcttccttc aggcgcacgc cgagggcgag
      961 acgatccttg agctcctgct ggtgttcgct ggcgaacgcg aagacggtgt tgcggccctc
     1021 ggttttcgca cggcgcatcg ccgactcggc ctggtgcagc aactcgtcgg cgcttgccgc
     1081 attgtcgggc aggcagctga cgccgatgct gcaggaaatg tagagatcgg tgtcgatcgg
     1141 ctcctcgacc cgggcgcgca cgctttcgcc gaattcagcc tggtcctgga tgcgcgtcgg
     1201 atccttgagc acgaggacga actcgtcgct ggcgacgtgg ccgacgatgc ccatttcacc
     1261 gacgacatcg accagcctgc ggccgaccgt cgccagcacg cggtcgcctg cggcgcggcc
     1321 gcgcatctcg ttcaacgcgt ggaagcgatc gaggttgatg tagagcacga cgacgcggct
     1381 gtcctgctgc agcgcctgga tgcaggcggc ctcgagaaat tcggtgatga tgccgatgcg
     1441 gggcaggccg cttgcggcat tgtggttggc caggaacgcg acgcgcgcct gggcggcgcg
     1501 ttgttcggtg acgtcttcgg cgagcgaagc ggtgccgatc ggtgcgtcgc cgtcgcgcag
     1561 cagggtgtgg ctccagcgca gcatgcggcg ttcgcccgag cggatgacga tttcgccctc
     1621 gtggtggcga tttgcgttgg cttccgcgag ccgttcctcg aatcgctgcc gccgctgctg
     1681 gcgatcggat tccgccacga agagcttgaa gtagtcctgg ccggaaagct ccatgaccgt
     1741 ccagccggtc aggcgggcga agcattcgtt gcaatagacg atgcggccgc ggtggtcga
//
LOCUS       DASRVO010000214         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_239767, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000214 DASRVO010000000
VERSION     DASRVO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239767"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFF05_02580"
     CDS             complement(<1..429)
                     /locus_tag="VFF05_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZW50719.1"
                     /translation="MSGFIRVWDLPTRAFHWMLAVLIALQYATGEFHFLDMRWHFWFG
                     YATLVLIAFRVLWGFLGSQTSRFADFLRGPAAVWRYLRSQSSTNLQTRVGHNPLGGWS
                     VIALLASVSVQSVSGLFASDEIDTDGPFVAHASDATVKLMT"
     gene            complement(426..>1719)
                     /gene="murD"
                     /locus_tag="VFF05_02585"
     CDS             complement(426..>1719)
                     /gene="murD"
                     /locus_tag="VFF05_02585"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZW50720.1"
                     /translation="GRAALAVLRKRFPDKPLALLCSEDEKAQVAADANLRLVPGPVDA
                     PSLSAYDVVIKSPGISPYKSPIPEAIAAGVKFTSGTALWFAERPDARVIAVTGTKGKS
                     TTSALIAHLARALRVRTALAGNIGLPLLELPDAEAQLWVVELSSFQTSEAGPLDVGVI
                     TNLYEEHLDWHGTRERYFADKLKLVDAARTLVVNAGQPELLARTVAHPRRVRFGDPAG
                     WHVEGDAIRRADTVVLPLAEIPLPGAHNAMNVCAALAALEAAGLDAIAAARHVNRFKP
                     LPHRLQKLGAHDGVEYVNDSISTTPHATIEALRSLHGRAVTVLVGGFDRGVDWSPFAN
                     YVAKRPPHAIVAMGANGARIGESLHAAQFDVVQVTTLAEAVQRAKTSTADGGVILLSP
                     GAPSFDQFKDYAERGREFARLAGFDPGSIAQIEGLGIA"
BASE COUNT          322 a          554 c          560 g          283 t
ORIGIN      
        1 cgtcatcagt ttgacggtgg catcggatgc atgcgcgacg aagggcccgt cggtgtcgat
       61 ctcgtcgctg gcgaacaatc cgctgacgct ctgcacggac acgctcgcga gcaaggcaat
      121 caccgaccag ccgccgagtg gattgtggcc cacgcgcgtc tgcaggttcg tggaagattg
      181 cgaacgcaga tagcgccaca ccgcggcggg tccacgcagg aaatcggcaa agcggctggt
      241 ctgcgagccg aggaatcccc acagcacgcg gaatgcgatc agcacgagcg tggcgtagcc
      301 gaaccagaaa tgccagcgca tgtcgaggaa atggaattcg ccggtcgcgt attgcagcgc
      361 aatcaacacg gcgagcatcc agtggaacgc tcgcgtgggc aaatcccaga cccggatgaa
      421 tccgctcatg cgattcccaa cccttcgatt tgcgcaatgc tgccgggatc gaatccggcg
      481 aggcgcgcga attcgcggcc acgctcggcg taatccttga attgatcgaa actcggcgcg
      541 cccggcgaaa gcaggataac gccgccgtcg gccgtcgatg ttttcgcacg ctgcacggct
      601 tcagccaatg ttgtcacctg cacgacgtcg aattgcgcag catgcaagga ctcgccgatg
      661 cgcgcgccgt tcgcgcccat cgcgacgatc gcgtggggcg ggcgtttggc gacgtagttc
      721 gcgaacggac tccaatcgac gccgcgatcg aatccgccga caagtaccgt gacggcgcgg
      781 ccatgcaggc tgcgcaacgc ctcgatggtc gcatgcgggg tggtcgagat gctgtcgttg
      841 acgtactcga cgccatcgtg cgcgccgagt ttctgcaagc gatgcggcag cggcttgaaa
      901 cgattgacgt gcctggcggc ggcaatggca tcgagtcctg ccgcttccag cgcggcgagc
      961 gcggcgcaga cattcatcgc attgtgcgca ccgggaagcg ggatctccgc cagcggcaaa
     1021 accacggtgt ccgcgcgccg tatcgcatcg ccttctacat gccagcccgc cggatcgccg
     1081 aaccgcacgc gtcggggatg cgccaccgtg cgtgcgagca attccggttg cccggcgttg
     1141 acgaccaacg tgcgcgccgc atccacgagt ttgagcttgt cggcgaaata gcgctcgcgc
     1201 gtgccatgcc agtcgagatg ttcctcgtac aggttcgtga tcacgccgac atccagtggc
     1261 ccagcctcgc tggtctggaa actggacagc tccacgaccc aaagctgcgc ttcggcgtcg
     1321 ggcagttcga gcagcggcaa gccgatattg ccggccagcg cggtgcgcac gcgcagcgcg
     1381 cgcgccagat gcgcgatcag cgcgctggtg gtgctcttgc ccttggtgcc ggtaacggcg
     1441 atgacgcgcg cgtcgggtcg ctcggcgaac cacagcgccg tgccggacgt gaatttcacg
     1501 cccgcggcaa tggcttcggg aatcggcgat ttgtacggcg agatgccggg agatttgatc
     1561 acgacgtcgt atgccgacag tgacggcgca tccaccggcc caggaacaag gcgaagattc
     1621 gcatcggccg cgacctgtgc cttctcgtct tcggaacaca acagcgccag aggcttgtcc
     1681 ggaaagcgct tccgcaacac cgccagcgcg gcgcggcct
//
LOCUS       DASRVO010000215         2339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_369506, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000215 DASRVO010000000
VERSION     DASRVO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369506"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="VFF05_02590"
     CDS             <1..1180
                     /locus_tag="VFF05_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZW50721.1"
                     /translation="YMGSRQRDTGAVDFVIAWLAEGELTQESYVNLIPTVQGGTHVNG
                     LRSGLTEAMREFCDIRNLLPRGVRLAPEDVWDRLCFVLSLKIQDPQFSGQTKERLSSR
                     DAAGFVENAAHDGFSLFLNQHVELGEKIAAIAIERASLRLKAEKQIVRKKITQGPALP
                     GKLADCASSDLSRTELFLVEGDSAGGSAKQARDKDYQAILPLRGKILNTWEVESGQVL
                     SSQEVHDLAVAIGCDPGKDDIAGLRYGKIIVLADADSDGLHIATLLSALFLRHFPTLV
                     REGHVFVAMPPLFRVDVGKQVFYCLDEAERDAQLARIEREKIKGAVNVTRFKGLGEMN
                     PAQLRESAMHPDTRRLVQLTVDVDDGTAPLMDMLLSKKRAADRKEWLETKGDLASLEV
                     "
     gene            complement(1187..1963)
                     /gene="hutG"
                     /locus_tag="VFF05_02595"
     CDS             complement(1187..1963)
                     /gene="hutG"
                     /locus_tag="VFF05_02595"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HZW50722.1"
                     /translation="MDIFTLHRGTSPLFVSLPHDGTTLPDDIAARMTLPARRVPDTDW
                     HVSRLYAFARELGASIIAPKHSRYVVDLNRPSDDVPLYPGQNTTGLCPIVQFSGEPVY
                     LPGQEPSADEIAQRVDKFWRPYHEALAAEIVRIKVAHGRVVLWEGHSIRSVVPFLFDG
                     RLPDFNLGTANGASCSADLQRRLIGVIEAQKKYTFVANGRFKGGYITRRYGDPANGID
                     AVQLELAQLNYMDEASFAYRDDLAAPTQAVIMALLETALV"
     gene            complement(1969..>2339)
                     /locus_tag="VFF05_02600"
     CDS             complement(1969..>2339)
                     /locus_tag="VFF05_02600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZW50723.1"
                     /translation="RAVCVTAWDSGYRLGSTMEFAGYDATLNRARLDALRRGAAEYLI
                     EPEGPAVVEEWYGWRPMTYDDLPILGRTASLQNVVLATGHGMLGITMSAITGKLIADL
                     ICGAPPILDLSPFSPARFNI"
BASE COUNT          447 a          742 c          754 g          396 t
ORIGIN      
        1 gtacatgggc tcgcgccagc gcgacaccgg tgccgtcgat ttcgtcatcg cctggctggc
       61 ggaaggcgag ttgacccagg aaagctatgt caacctgatt ccgaccgtgc agggcggcac
      121 gcacgtcaac ggcctgcgca gcggcctcac cgaggcgatg cgcgagttct gcgacatccg
      181 caacctgctg ccgcgcggcg tccggctcgc gcccgaggat gtctgggacc gcctgtgctt
      241 cgtcctgtcg ctgaagatcc aggatccgca attctccggc cagaccaagg agcgcctgtc
      301 ctcgcgcgac gccgccggct tcgtcgaaaa cgccgcgcac gacgggtttt cgctgttcct
      361 caaccagcac gtggaactcg gcgagaaaat cgccgcaatc gccatcgaac gcgcgtcgct
      421 gcggctgaag gccgaaaagc agatcgtgcg caagaagatc acgcagggac cggcgctacc
      481 cggcaagctc gccgattgtg cgtccagcga cttgtcgcga accgaactgt tcctcgtcga
      541 gggcgattcg gccggcggca gcgccaagca ggcgcgcgac aaggattacc aggccatcct
      601 gccgctgcgc ggaaaaatcc tgaatacgtg ggaagtggaa tccgggcagg tgctgtcttc
      661 tcaggaagtg cacgacctcg ccgtcgccat cggctgcgat ccgggcaagg acgacatcgc
      721 cggactgcgc tacggcaaga tcatcgtgct cgccgacgcg gactccgacg gcctgcacat
      781 cgccacgttg ttgtccgcgt tattcctgcg tcacttcccc acgctggtgc gcgaaggcca
      841 cgtcttcgtc gccatgccgc cgctgttccg cgtcgacgtg ggcaagcagg ttttctattg
      901 cctcgacgaa gccgagcgcg acgcgcaact ggcccggatc gagcgcgaga agatcaaggg
      961 cgcggtcaac gtcacccgct tcaaggggct cggcgaaatg aacccggcgc aactgcgcga
     1021 atcggccatg cacccggata cgcgccgcct cgtgcaactc accgtcgatg tggacgacgg
     1081 cactgcgccg ctgatggaca tgctgctctc gaagaagcgc gccgccgacc gcaaggaatg
     1141 gctggagaca aagggtgatt tggcaagtct ggaagtctga cgccgatcaa accagcgctg
     1201 tttcgagcag cgccatgatg actgcctgcg tcggcgcggc gaggtcgtcc cgatacgcga
     1261 acgaagcctc gtccatgtag ttcaactgcg ccagttcgag ctggaccgcg tcgattccgt
     1321 tcgccgggtc gccgtagcgc cgcgtgatgt aaccgccctt gaatcgaccg ttggcgacga
     1381 aagtatattt tttctgtgct tcgatcacgc cgatcagtcg acgttgcaaa tccgcggaac
     1441 aactggcgcc gttggcagtg cctaggttga aatccggcag gcgtccgtcg aacaggaacg
     1501 gaacgacgct acggatggag tggccttccc agagcacgac gcggccatgc gcgaccttga
     1561 tgcgcacgat ttccgcggcg agcgcctcgt ggtacggacg ccagaatttg tccacgcgtt
     1621 gcgcgatttc atccgccgat ggttcctgac cgggcagata caccggctcg ccgctgaact
     1681 gcacgatcgg gcacagcccc gtggtgttct gaccgggata cagcggcacg tcatccgatg
     1741 ggcgattcag atcgacgaca tagcgcgaat gcttcggcgc gatgatcgac gcgccgagct
     1801 cgcgcgcgaa cgcgtacagg cgcgacacat gccaatccgt atccggcacg cggcgggcag
     1861 gcagcgtcat gcgcgcagcg atgtcgtcag ggagcgtcgt gccatcgtgc ggcagcgaga
     1921 cgaacagtgg cgacgtgccc cgatgcaggg tgaagatatc catgagcgtc agatattgaa
     1981 gcgtgcggga gagaacggcg acaagtcgag tatgggcggc gcaccgcaaa tcaggtcggc
     2041 aatgagtttt ccggtaatcg ccgacatggt gatgccgagc atgccgtgtc cggtggcgag
     2101 gacgacattt tgcagcgacg cggtacgacc caggatcggc aggtcatcgt acgtcatcgg
     2161 ccgccagcca taccattcct cgacgacagc cgggccttcg ggttcgatca gatattcggc
     2221 ggcgccgcgc cgcagcgcat ccaggcgcgc gcgattgagc gtggcgtcgt agccggcaaa
     2281 ttccatcgtg ctgccgaggc gataaccgga gtcccatgcc gtcacgcaga cggcgcgtt
//
LOCUS       DASRVO010000216         1016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_369515, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000216 DASRVO010000000
VERSION     DASRVO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369515"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..704)
                     /locus_tag="VFF05_02605"
     CDS             complement(162..704)
                     /locus_tag="VFF05_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZW50724.1"
                     /translation="MDHSPDAASGTSAMLGTDALFVAVYARLKAMAAHELKGANATLN
                     TTGLVHEAYLRMGKQPDLAFEHPAQFFVYAARAMRHLLADRARDRLRLRAGGGLMHIT
                     LTGSDSQLAIDSAEQALALDAALDRLAQSDARAARVLELHYFAGLTVAQIADMMQLAT
                     STVDRDWRFARAFLKDELAG"
     gene            complement(802..>1016)
                     /locus_tag="VFF05_02610"
     CDS             complement(802..>1016)
                     /locus_tag="VFF05_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZW50725.1"
                     /translation="GGVIALGTLTIRDQSKPVTLNVKFAVSGGATTLDVDSTLNRLDF
                     GLGTSGDWADISRDIHVHGHLVLSAK"
BASE COUNT          173 a          321 c          336 g          186 t
ORIGIN      
        1 agggtcgcat cggcgcagtg cgccgacaga tacgctcgac gttccgccgg cgtcaggtcg
       61 gggcacgctt cgaacagatc gcgcagcgtt ggcgtggttg caggcatgcg tgggtacttt
      121 tttccgctac ttcgaacaac gccgcttggc cgcgtggcgc gtcatccggc caactcgtcc
      181 ttgaggaaag ctcgagcaaa acgccagtcg cgatcgacgg tactggtggc cagttgcatc
      241 atgtcggcaa tctgcgccac cgtcagtccc gcgaaatagt gcagctccag cacgcgcgct
      301 gcgcgtgcat cggattgtgc gaggcgatcc agcgcggcgt ccagcgccaa cgcctgctcg
      361 gcgctgtcga tcgccagttg ggagtcgctg ccggtgagcg tgatgtgcat cagaccgcct
      421 ccggcacgca aacgcaggcg atcgcgtgcg cgatcggcaa gcaggtgacg cattgcgcgt
      481 gcggcgtaaa caaagaattg cgccggatgc tcgaacgcca aatccggctg cttgcccatg
      541 cgcaaatacg cctcgtgcac caggcccgtg gtattgaggg tggcgttcgc acccttaagt
      601 tcgtgcgcgg ccatggcctt gaggcgagcg taaaccgcga cgaacagcgc atcggtaccg
      661 agcattgcgc tggtgccgct cgccgcgtcg ggtgaatggt ccatggcgta cctccgagtc
      721 ggattctacc gccagcacgc tcaacgccgc cgcggatcga tttctcgcag cgcgcacgag
      781 ggtcgcgtcg tcgataggcg tttatttcgc cgacagcacc agatgcccgt gcacatggat
      841 gtcgcggctg atatcggccc agtcaccgct cgtgccgagg ccgaaatcga ggcggttgag
      901 cgtgctgtcg acgtcgagcg tcgtggcgcc gccggacacc gcgaacttta cattcagcgt
      961 gacgggtttg ctctggtcgc ggatggtgag cgtgcccagc gcgatcacgc cgccgt
//
LOCUS       DASRVO010000217         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_6535, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000217 DASRVO010000000
VERSION     DASRVO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6535"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VFF05_02615"
     CDS             <1..1242
                     /locus_tag="VFF05_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW50726.1"
                     /translation="LGAVPRGQSVTRYWRWVLAGVTVVLLVLTYGWMRSGQQQPAPLY
                     RTEVVQRGDLTVTVSATGNVQPTNQVDVGSELSGIVESVFVEENDRVQHGQVLAQLDV
                     SRLKDQIVKSQGAVTAAKAQVDQTVATVKEKRLAFERLKGVAALSHGGIPSKADLDTA
                     DAALQRAIADEAAARANVVQASATLRSDQTSLAKASIRSPIDGVVLTRKVEPGQTVAA
                     SLQAPVLFTLAENLSQMQIQVDVDEADVGQVRDGQEATFTVDAYPNRKYPARISRVDY
                     GSQVKDGVVSYLTVLTVNNDDLSLRPGMTATAEITTIQVRDALLVPSGALRFTPPDTS
                     ASASKSSGGITSMLMPRIPRAAQQQKSAAGMQRVWILRDGQPVAISVKTGATDGRQTQ
                     ITEGSLQPGVAVITELASAAR"
     gene            1239..1961
                     /locus_tag="VFF05_02620"
     CDS             1239..1961
                     /locus_tag="VFF05_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZW50727.1"
                     /translation="MSLDSGTADTPLIELIGVTKTYGRGAAELQALRGVDLTVRAGEF
                     MAIMGPSGSGKSTVMNILGCLDVPTTGAYRFRGIHVERLTRNQRALLRRNFLGFVFQG
                     YNLLARTSALENVELPLLYRGEQAAVRHAVAHEALASVGLSEWETHTPAELSGGQQQR
                     AAIARAIVTHPEVLLADEPTGNLDTQRSREIMDLIAKLNRERGITVLMVTHEPDMAAY
                     AKRVVHFIDGRVDSDVRNGERP"
     gene            1958..>2678
                     /locus_tag="VFF05_02625"
     CDS             1958..>2678
                     /locus_tag="VFF05_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW50728.1"
                     /translation="MMGFTGFLLSLREIQRNLLRSFLTILGIVIGVAAVITMVTLGNG
                     ATKAVSDQISSLGSNLLIVRPGQRFGAGHGSAPPFRAADVRAISDQIGSLQAVAPSVQ
                     RSATVVRLANNWTTVIGGSTNAYFTARQWQPAIGRIFTETEERAGKAVCVIGETVRRE
                     LFGQENPIGGRIRIKQFSCEVIGLLAPKGQAAMGQDQDDTIMMPLRTVQRRLAGNQDV
                     NALMISVADGASIDRAKSQIEK"
BASE COUNT          502 a          837 c          899 g          440 t
ORIGIN      
        1 ctcggggcgg tgccgcgcgg ccaatccgtg acgcggtact ggcgctgggt gctcgcaggc
       61 gtgacggttg tgctgctggt gctgacgtat gggtggatgc gctcgggaca gcagcagcca
      121 gcgccgcttt atcgcacgga agtcgtgcag cgcggcgact tgaccgtcac cgtttcggcc
      181 accggcaatg tgcaaccgac caaccaggtc gatgtcggca gcgagttgtc cggcatcgtc
      241 gagtccgtct tcgttgaaga gaacgatcgc gttcagcacg gtcaggtgct cgcccaactc
      301 gatgtgtcac gcctgaagga tcaaatcgtc aagtcgcaag gcgcggtgac cgccgcgaag
      361 gcacaggtcg atcagactgt cgcgacagtc aaggaaaaac gtctggcgtt cgagcgcctg
      421 aagggcgtgg cggcgctgag tcacggcggc attccgtcga aggccgatct cgatacggcc
      481 gacgcggccc tgcagcgtgc gatcgccgac gaggcggcgg cacgcgcgaa cgtggtgcag
      541 gcgagcgcga ctctgcgctc cgaccaaacc agtctggcga aggcatcgat ccgctcgccg
      601 atcgacgggg tcgtgttgac gcgcaaggtc gaaccgggcc agaccgtcgc cgcgtcgctg
      661 caggcgccag tgctgttcac gctcgccgag aacctgtcgc agatgcagat acaagtcgat
      721 gtcgacgaag cagacgtcgg tcaggtgcgt gatggccagg aggcgacgtt tacggtcgat
      781 gcatatccga atcgaaaata tccggcgcgc atcagccgcg tcgactatgg ttcgcaggtc
      841 aaggatggcg tcgtctcgta cctgacggtg ctcacggtca acaacgacga tctgtcgctg
      901 cgtccgggca tgaccgcgac cgctgagatc acgacgatcc aggtgcgcga cgcgttgctg
      961 gtgccaagcg gcgcgctgcg cttcacgccg ccggacacgt ccgcaagcgc gtcgaaatcg
     1021 agcggtggaa tcaccagcat gcttatgccg cgcatcccgc gagccgcgca gcaacagaag
     1081 tcggctgctg gcatgcagcg cgtttggatt ctgcgcgatg gccagccggt cgcgatcagc
     1141 gtcaagaccg gcgctaccga cggccgtcag acacagatta ccgagggcag cctgcagcca
     1201 ggagtcgcgg tcatcacgga actggcgagt gcggcgcgat gagtctggac tccggaacgg
     1261 ccgacacgcc gttgatcgag ctcatcggcg tcacgaagac ctacggtagg ggagccgcgg
     1321 agctgcaggc attgcgcggc gtcgatctga ccgtgcgcgc cggcgagttt atggcgatca
     1381 tgggaccaag cggctctggc aaatcgacgg tcatgaacat cctcggttgc cttgacgtgc
     1441 cgacgacagg cgcgtaccgg tttcgcggga tccacgtcga gcgccttacg cgcaatcagc
     1501 gcgcattgct gcgtcggaat tttctcggct tcgtcttcca gggctacaac ctgctcgcgc
     1561 gcacatccgc gctcgaaaat gtcgagttgc ctttgctcta ccgcggcgag caggccgctg
     1621 tccggcatgc cgtcgcgcac gaggcgctcg cgtcggtcgg cttgagtgaa tgggaaacgc
     1681 acacgccggc cgagctttcc ggcggacagc aacagcgtgc ggcgatcgcg cgcgcaatcg
     1741 tcacgcatcc ggaggtgctg ctcgcggacg agccgaccgg aaatctcgat acgcagcgca
     1801 gccgcgaaat catggacctg atcgccaagc tgaaccgcga gcgcggcatc acggtgctca
     1861 tggtcacgca cgaaccggac atggccgcgt acgccaagcg cgtcgtgcac ttcatcgacg
     1921 gtcgcgtcga cagcgacgtg cgcaacggag agcggccatg atgggcttta cggggttcct
     1981 gctgtcgcta cgcgagatcc agcgcaatct gctgcgctcg tttctgacga tcctcggcat
     2041 cgtcatcggc gtggccgccg tcatcacgat ggtcacgctc ggcaacggcg ccacaaaggc
     2101 cgtgtccgat cagatctcga gcctcggcag caatctgctg atcgtgcggc cgggacaacg
     2161 cttcggggca ggccacggca gcgccccacc gttccgggcc gcggatgtgc gcgcgatcag
     2221 cgatcagatt ggctcgctgc aggcggtcgc gccgtcggtg caaagaagcg cgacggtcgt
     2281 gcgcctggcc aacaactgga ccacggtcat cggcggcagc acgaacgcgt acttcaccgc
     2341 gcgccaatgg cagccggcca tcgggcgcat attcaccgaa accgaggaac gtgccggcaa
     2401 ggcagtctgc gtgatcggcg aaaccgtgcg tcgcgaattg ttcggccaag aaaatccgat
     2461 cggcggccga atccgcatca agcagttctc gtgcgaagtg atcggtctgc tggcgccgaa
     2521 aggccaggcg gccatggggc aggatcagga cgatacgatc atgatgccgt tgcgcacggt
     2581 gcagcggcgc ctcgcgggca atcaggacgt caatgcgctg atgatctcgg tcgccgatgg
     2641 cgcatcgatt gatcgcgcga agtcgcagat cgaaaaac
//
LOCUS       DASRVO010000218         1023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_239812, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000218 DASRVO010000000
VERSION     DASRVO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239812"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFF05_02630"
     CDS             <1..907
                     /locus_tag="VFF05_02630"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HZW50729.1"
                     /translation="LSPGEVLIKVAYSSVNYKDALAGTGKGKILREPVLNGGIDVAGY
                     VAASSDPNFKEGDQVLCTGCGLSETRDGGYAQYARLESKWTIPLPAGLTLREAMIVGT
                     AGFTAALSLYQMRRNGQVPEMGPIVVTGATGGVGSLAIDILTRAGFEAHAISGKIDRF
                     DDLIALGAKQCISRRDLYWGQRPLESAHWAGAIDNVGGDMLSGLTRVIKPYGNIASCG
                     LAAAAELQTTVMPFIIRGLSLLGIASAGTARDIRDEVWAHLGSDWKPAHLERIATREV
                     GMTELPGVFDIMLAGGSFGRTVVKI"
BASE COUNT          195 a          327 c          322 g          179 t
ORIGIN      
        1 tctttctccc ggcgaagtgc tgattaaggt cgcgtactcg tcggtcaact acaaggacgc
       61 gctggccggc accggcaagg gcaagatcct gcgcgagccg gtgctcaacg gcggcatcga
      121 cgtggcgggt tacgtggccg catccagcga tccaaacttc aaggaaggcg accaggtgct
      181 ttgcactggt tgcggcctgt ccgagacgcg cgatggcggc tatgcgcaat acgcgcgcct
      241 cgaatcgaag tggacgatcc cgctgccggc cggactgacc ctgcgcgaag ccatgatcgt
      301 gggcaccgcg ggatttaccg cggcgctgtc gctgtaccag atgcgtcgca acgggcaagt
      361 tccggagatg ggaccgatcg tcgtcaccgg cgcgaccggc ggcgtcggat cgctcgcgat
      421 cgatatcctg acccgcgccg gtttcgaggc gcacgcgatc agcggcaaga tcgaccgctt
      481 cgacgacctg atcgcgctcg gcgcgaagca atgcatctcg cgcagggatc tgtactgggg
      541 ccagcgtccg ctggaatccg cgcattgggc cggcgccatc gacaacgtcg gcggcgacat
      601 gctttcaggg ctcacccgcg tgatcaagcc ctacggcaac atcgccagct gcggactcgc
      661 cgccgccgcg gaactgcaaa ccacggtgat gcccttcatc atccgcggcc ttagcctgct
      721 cggcatcgcc tccgcgggaa ccgcgcgcga catccgcgac gaggtctggg cacacctggg
      781 ttcagactgg aaaccggcgc acctggaacg cattgccacg cgcgaagtcg gcatgaccga
      841 attgcctggc gtgttcgaca tcatgctcgc gggcggctcg ttcggccgca ccgtggtcaa
      901 gatttgaccc gaaatcgatc tcgattcccg atcaagaaaa tgcgtcaaca aactggaatg
      961 caagaatttt ttttggtatc gagaatcgcg ctggaaatat cgtcgcgcag cgccttagaa
     1021 tgc
//
LOCUS       DASRVO010000219         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_84629, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000219 DASRVO010000000
VERSION     DASRVO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84629"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFF05_02635"
     CDS             complement(<1..1138)
                     /locus_tag="VFF05_02635"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZW50730.1"
                     /translation="MKRVAVLGATGSIGASALDVIARHPQRFAVAALSANRNVDALVA
                     LCVKHRPALAVIADPALESTLCEKLAAANLPTRAASGDAGLREAAAGDADIVIAAIVG
                     AAGLDSTLAAARAGKRLLLANKEAVVMAGPLLLQALREGGGELVPIDSEHNAIFQCLP
                     TSGLPGGHPDLDDAGVRRIVLTASGGPFRGRTRAQLAAVTPDQACAHPNWVMGRKISV
                     DSATLMNKGLEVIEAHWLFGASPDRIEVVVHPQSIVHSLVEYIDGSTLAQLGNPDMRT
                     ALAHALGFPERIESGVAGLDLARLGALQFETPDRATFACLDLAYRALAAGGTAPAILN
                     AANEIAVAAFLAGTLPFLAIAEVIAATLDAMPAEPVETLERLIYA"
     gene            complement(1135..1950)
                     /locus_tag="VFF05_02640"
     CDS             complement(1135..1950)
                     /locus_tag="VFF05_02640"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZW50731.1"
                     /translation="MKQRVVTALLLAPLAIALILLLPTPAFALVIVGLCLLALWEWTR
                     LSGLRDRRVRGILVALAAVGFYALWENRDAQATVVVIAIGCVWWVIAVAWLRQFSFAA
                     APTRENAVLKLVAGALCVFPAWIALMRIHAAPVHGNWWALYALLLGWSADTFAYLAGM
                     RWGRRKLAPAISPGKTWAGVYGGLVATAVVAAIGGCLLGVRNLDLFALVVLAMLCVCF
                     SVVGDLFESLIKRHAGVKDSGALFPGHGGVFDRLDGLFALLPLFALGKLLLGL"
     gene            complement(1947..>2542)
                     /gene="uppS"
                     /locus_tag="VFF05_02645"
     CDS             complement(1947..>2542)
                     /gene="uppS"
                     /locus_tag="VFF05_02645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZW50732.1"
                     /translation="TIAFCLRHGIEALTLFAFSSENWQRPKDEVGALMELFLKALDRE
                     VDELHGHGVRIAFIGDLSAFAPALRERMLAAMEKTCGNGKLALNVAVNYGGRWDIANA
                     AKQAALAVQRGEIAAEAIDEKSIARYFCLADLPPVDLFIRTGGEQRISNFLLWQSAYS
                     ELYFSDVLWPDFDAACLDQAITEFARRERRFGRVSSS"
BASE COUNT          479 a          831 c          847 g          385 t
ORIGIN      
        1 cggcgtaaat caagcgctcc agcgtttcga ccggttcagc cggcattgcg tccagcgtgg
       61 cggcgatgac ctcggcgatg gcgaggaagg gcagggtgcc ggccagaaac gccgccaccg
      121 cgatttcgtt cgcggcattc aggatcgccg gcgcggtgcc gccggcggcg agtgcacgat
      181 acgctaggtc cagacacgcg aaagtggccc ggtccggtgt ttcgaattgc agcgccccca
      241 agcgcgccag atcgagtccg gctacgcccg attcgatgcg ttccggaaat cccaatgcat
      301 gcgccagtgc ggtgcgcatg tccggattgc cgagttgcgc cagggtcgag ccgtcgatgt
      361 attcgaccag cgaatgcacg atgctttgcg gatgcacgac gacctcgatc cggtcgggac
      421 tggcgccgaa cagccagtgc gcctcgatca cctcgaggcc cttgttcatg agcgtcgccg
      481 aatccaccga aatcttgcgg cccatgaccc agttcggatg cgcgcaggcc tggtcgggcg
      541 taaccgcggc gagttgcgcg cgcgtgcgtc cgcggaacgg cccgcccgag gcggtcagca
      601 cgatgcggcg cacgccggca tcgtccaggt cgggatgccc gcctggcagg ccggacgttg
      661 gcaggcattg aaatatcgcg ttgtgctccg aatcgatcgg cacgagttcg ccgccgcctt
      721 cgcgcagggc ttgcaatagc agcgggccgg ccatgaccac cgcttccttg ttcgccagca
      781 gcaggcgctt gccggcgcgc gcggcggcaa gggtcgagtc cagacccgcc gcaccgacga
      841 tcgcggcgat gacgatgtcc gcgtcgcctg cggcagcctc gcgcaatccg gcatcgcctg
      901 aagcggcgcg cgtcggcagg tttgccgcgg cgagtttttc gcacagcgtg gattccagcg
      961 cgggatcggc gatcacggcc agtgccggac gatgtttcac gcacagcgcg accagcgcat
     1021 cgacattgcg attggcgctg agcgcggcga cggcgaagcg ctgcggatgg cgtgcgatca
     1081 cgtccagtgc gctggcaccg atcgagccgg tggcgccaag gaccgcgacg cgcttcataa
     1141 gccgagcagc agtttgccga gcgcgaacag cggcaacagg gcgaacaggc cgtcgaggcg
     1201 gtcgaacacg ccaccatggc cgggaaacag cgcgccggaa tccttgacgc cggcgtggcg
     1261 cttgatcagg ctttcaaaca ggtcgccgac cacggaaaag cacacgcaga gcatggcgag
     1321 cacgacgagc gcgaaaaggt ccaggttgcg cacgccgagc aggcagccgc cgatggcggc
     1381 gacaacagcg gtggcgacaa gccctccgta gacgccggcc caggtcttgc cgggactgat
     1441 cgccggtgca agtttccgcc ggccccagcg catgcccgcg aggtaggcga acgtatccgc
     1501 cgaccagccg agcaacagcg catacaacgc ccaccagttt ccatgcaccg gtgcggcgtg
     1561 aatgcgcatc agggcgatcc aggcgggaaa aacgcagagc gcgccggcaa cgagtttcag
     1621 tacggcgttt tcccgcgtcg gcgctgcagc aaaactgaat tgcctcaacc acgccaccgc
     1681 gatcacccac cacacgcatc cgatcgcaat gacaacgacc gtggcctgcg catcgcggtt
     1741 ttcccacaat gcgtagaagc ccacggctgc aagcgcgacc aggatgccgc gcacacggcg
     1801 gtcgcgcagg ccggacaggc gcgtccattc ccacaaggcg agcaggcaca agccgacgat
     1861 gacgagggcg aatgccggtg tcggcaacaa tagaatgagc gcgatggcca gcggggccag
     1921 caacaaggcg gtgacgacgc gctgcttcat gaagaggaaa ctctcccgaa tctgcgctcg
     1981 cgccgcgcga attcggtgat cgcctgatcg aggcaggcgg cgtcgaaatc cggccacagt
     2041 acgtcgctga aatacagttc gctataggcg ctttgccaaa gcaggaaatt gctgatgcgc
     2101 tgttcgccgc cggtgcggat gaacaggtcc accggcggca ggtcggcaag gcagaaatat
     2161 cgcgcgatgg atttttcgtc gatggcttcg gcggcgattt cgccgcgctg cacggcgagc
     2221 gcggcctgct tcgctgcatt ggcgatgtcc cagcgcccgc cgtagttcac ggcaacattc
     2281 agcgcgagct tgccgttgcc gcaggttttt tccatcgccg ccagcatgcg ctcgcgcaag
     2341 gccggtgcga acgcggacaa atcgccgatg aaggcgatgc gcacgccatg gccgtgcagt
     2401 tcgtcgactt cgcggtcgag cgccttcagg aacaattcca tcagcgcgcc gacctcgtct
     2461 ttcggccgtt gccagttctc gctggagaac gcgaacaggg tcagcgcctc gatgccgtgg
     2521 cgcaggcaga acgcgatcgt cg
//
LOCUS       DASRVO010000220         1055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_369666, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000220 DASRVO010000000
VERSION     DASRVO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369666"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFF05_02650"
     CDS             <1..410
                     /locus_tag="VFF05_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HZW50733.1"
                     /translation="PLARAMQLRLRDYDGDRLTIAAPLAPNINDKGCAFGGSLVSLLT
                     LAGWSLIVLKLRALGSACDVYVQDSTVRYLAPVWDDFIAQARLAEGESWETFASTLAA
                     RGRARLTVDCRVPLADGADACTLQARFVAISKP"
     gene            462..881
                     /locus_tag="VFF05_02655"
     CDS             462..881
                     /locus_tag="VFF05_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50734.1"
                     /translation="MRTILLVCGFALLSGCAAMTAQSESRALELTLNAYANAIRWGDI
                     NQAVPFVDPETMRKHPLSQLDIDRYKQIHFASYNEQPPVPAGPHEVRQVVQMSLVNVN
                     TQVERSIVDNQLWRYNEKTKHWLLVSGLPDITQHSTP"
BASE COUNT          185 a          367 c          315 g          188 t
ORIGIN      
        1 tcccgctggc gcgcgccatg caattgcgcc tgcgcgacta cgacggcgat cgcttgacca
       61 tcgccgcgcc gctagcacca aacatcaacg acaagggctg tgccttcggc ggcagcctgg
      121 tgagtctgct caccttggcc ggctggagcc tgatcgtgct caagctgcgc gcgctcggca
      181 gcgcctgcga cgtgtacgtg caggacagca ccgtgcgcta ccttgctccg gtgtgggacg
      241 acttcatcgc gcaagcgcga ctggccgaag gcgaatcctg ggaaacattc gcgtccacgc
      301 tcgccgcacg cggtcgcgcg cgcctgaccg tggattgccg cgtgccgctc gccgacggcg
      361 cggatgcgtg caccttgcag gcgcgcttcg tggcgatttc caaaccctga atcggcgcgc
      421 gatttgcggc acaatgccgc gtcgccgccg gagtaacgat catgcgcacc atactgctcg
      481 tttgcggatt cgccctgctc tccgggtgcg cggcgatgac cgcgcaatcg gaaagccgcg
      541 cgttggagct gaccttgaac gcctacgcga atgcgatccg ctggggcgac atcaaccagg
      601 cagtcccgtt cgtcgatccg gaaaccatgc gcaagcatcc gctgagccaa ctcgacatcg
      661 accgctacaa gcaaatccat ttcgcctcat acaacgagca accgcccgtg ccggccggcc
      721 cgcacgaggt gcgccaggtc gtgcagatga gcctggtcaa cgtcaatacc caggtcgaac
      781 gcagcatcgt cgacaaccag ttgtggcgct acaacgagaa gacgaagcac tggctgctgg
      841 tgtcgggact gcccgacatc acccaacatt cgacgccgta acgctggcat ggaccggcgc
      901 gctgccgcat aatccgcggc ctttccccgt gtgcgccacg ccatgtcgct gtttctgcaa
      961 cgcctgccgg aattcctggc ccaccatgtt tttctcgcgc tggctttcgc cggtttgatc
     1021 gcggcgatcc tcggccacga aatcgcacgc cgttt
//
LOCUS       DASRVO010000221         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_214365, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000221 DASRVO010000000
VERSION     DASRVO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214365"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VFF05_02660"
     CDS             complement(<1..221)
                     /locus_tag="VFF05_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-epi-5-epi-valiolone synthase"
                     /protein_id="HZW50735.1"
                     /translation="MIESPHLFDPANAALLAFGRPGGRRFVVVDAEVARHYGTTIRAF
                     FDRHRIEARIAVFPGGEEHKSVEAWQDILR"
     gene            complement(269..757)
                     /locus_tag="VFF05_02665"
     CDS             complement(269..757)
                     /locus_tag="VFF05_02665"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HZW50736.1"
                     /translation="MIDTKSLGWRDDLVGEMDHRRLKAPSVKLRMAREGASGEAIYCV
                     DLRVRRPNAGEFLSLTELHSMEHFLLEGFSRLLPEAFVSVGIMGCQTGFYLVFRNEGR
                     AGELCNVLERILEQMQGATSVAYANVEQCGNWRNHSLADAQRIAREILARRSDWLDAA
                     CA"
     gene            complement(786..>1638)
                     /locus_tag="VFF05_02670"
     CDS             complement(786..>1638)
                     /locus_tag="VFF05_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HZW50737.1"
                     /translation="IDPGELLADSIRECLREIHDDEDITLLVEPEPGMYIETLAQGLA
                     LIRRVDSPKFRLHIDICHAYCSEPDYIRALHEAAPQARYLHISDARAGYNLRVVEDRD
                     GFSPDLGIAAALVHFPDTCEFLLLDHDRPTYFRDTVPTPGRKKRIDELLQCCGVNNDL
                     AVVDYSGLFAGRSALDDEIFTWMISVPGISFDVLERARPIVNYLRGARSTALVNRMVA
                     NTLTGMVHFHEIPGQGTLDFAASFKALTDAGFRGYGSVELYHHVASWQQALNDSYRHL
                     APLVAAH"
BASE COUNT          308 a          534 c          511 g          285 t
ORIGIN      
        1 cgcaggatat cctgccaggc ctcgaccgac ttgtgctctt cgccgcccgg aaacaccgcg
       61 atccgcgcct cgatccgatg tcgatcgaag aacgcgcgga tcgtcgtgcc gtaatggcgc
      121 gcgacttccg cgtccaccac cacgaagcga cgcccgcccg gccgcccgaa ggcaagcagc
      181 gccgcgttcg ccgggtcgaa caggtgcggc gactcgatca cgtcataggc gatcggtcgt
      241 tgcgtgcgca cctgccagca cctggcgatc atgcgcatgc cgcatccagc cagtcgctgc
      301 gccgtgcgag gatctcgcgc gcaatgcgct gcgcatcggc gaggctgtga ttgcgccagt
      361 tgccgcattg ctcgacattg gcgtacgcca cgctcgtcgc cccttgcatc tgctccagaa
      421 tccgttcgag cacgttgcaa agttcgcccg cgcgcccttc gttgcggaac acgagataga
      481 acccggtctg acagcccatg atgccgacgg aaacgaacgc ctccggcagc aggcgcgaaa
      541 atccctcgag caggaaatgt tccatcgaat gcagttcggt caaggacagg aactctccgg
      601 cattgggccg gcgcacgcgc aggtcgacac aatagatcgc ctcgcccgag gcaccctcgc
      661 gcgccatgcg caacttgacg ctcggcgcct tgagccggcg gtgatccatt tcgccgacga
      721 ggtcatcgcg ccagcccagg gatttggtgt cgatcatgaa ttcggttcgc gagattccac
      781 tacgctcaat gcgccgcgac gagcggagcc agatgccggt aactatcgtt gagtgcctgc
      841 tgccacgacg ccacgtggtg atagagctcg accgagccat agccgcggaa tccggcatcg
      901 gtcagcgcct tgaagctggc cgcgaaatcc agcgtccctt gccccggtat ttcgtggaaa
      961 tgcaccatgc cggtcagcgt gttggcgacc atgcgattca ccagcgctgt gctgcgggcg
     1021 ccacgcaggt agttgacgat cggacgcgcg cgctcgagca cgtcgaagga aatgccgggc
     1081 acggaaatca tccaggtgaa gatctcgtcg tccagcgccg accttcccgc aaacagtccg
     1141 gaatagtcga cgacggcgag gtcgttgttc acgccgcagc actgcagcaa ttcgtcgatg
     1201 cgcttcttcc tcccgggcgt cggcacggta tcgcggaagt aggtcgggcg atcgtgatcc
     1261 agcagcagga attcgcatgt atcggggaaa tgcaccagcg cggccgctat gccgagatcg
     1321 ggtgaaaagc cgtcgcgatc ctcgaccacc ctgaggttgt agccggcgcg cgcgtcggaa
     1381 atatgcaggt agcgcgcctg cggtgccgcc tcgtgcagcg cgcgtatgta atcgggttcc
     1441 gagcaatagg cgtggcagat gtcgatgtgc agcctgaatt tgggcgaatc cacgcggcgg
     1501 atgagcgcaa gaccctgcgc cagcgtctcg atgtacatgc ccggctccgg ctcgacgagc
     1561 agcgtgatgt cctcgtcatc gtggatctcg cgcaagcact cgcggatgga atccgccagc
     1621 aactcgcccg gatcgatt
//
LOCUS       DASRVO010000222         1913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_162285, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000222 DASRVO010000000
VERSION     DASRVO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162285"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /gene="ychF"
                     /locus_tag="VFF05_02675"
     CDS             complement(<1..944)
                     /gene="ychF"
                     /locus_tag="VFF05_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HZW50738.1"
                     /translation="MGIKCGIVGLPNVGKSTLFNALTKAGIAAANFPFCTIDPNIGVV
                     PVPDPRLNELAEIVKPQKLVPTAVEFVDIAGLVAGASKGEGLGNKFLAHIREVDAIAH
                     VVRCFEHPDIVHVAGKIDPIADIETIDTELALADLESVDKALNRAERAAKANDKEALA
                     KRPVLQKLATGLNEGKSARSLGLDDDEKALVRDLFLLTLKPLMYIANVKEDGFANNPY
                     LDKVRARAAAEGAEVVPVCAAIEEELSQLDDQDRDAFLKDMGLDEPGLNRVIRAAYKL
                     LGLQTYFTAGEKEVRAWTVKRGSTAPQAAGVIHTDFARG"
     gene            complement(952..1533)
                     /gene="pth"
                     /locus_tag="VFF05_02680"
     CDS             complement(952..1533)
                     /gene="pth"
                     /locus_tag="VFF05_02680"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZW50739.1"
                     /translation="MAGLRLLVGLGNPGAEHLRTRHNAGFWFVDALAQREGARFGLET
                     KLHGETAKVVLDGQPLLLLKPNTFMNKSGIAVASALRYWKIAPEEMLVAHDDLDLPPG
                     AARLKFDGGHGGQNGLRDIFAHLGHGEFHRLRIGIGHPGHKDRVTPWVLGRPGKADED
                     AILDAVGAALDVLPLAVAGDFNEAMKRLHTAQE"
     gene            complement(1591..>1913)
                     /locus_tag="VFF05_02685"
     CDS             complement(1591..>1913)
                     /locus_tag="VFF05_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HZW50740.1"
                     /translation="NDVEIACLPKDLPEHIEVDLSELKVGDIVHLSALKLPNGVEIPE
                     LRLGKEHDTAVVTAQEMKQEVEVAPVVEAAGATGAAPAAGATGAAPAGGAAAPAAAKK
                     DEKK"
BASE COUNT          298 a          639 c          596 g          380 t
ORIGIN      
        1 ccgcgcgcga aatcagtgtg gatgacacct gcggcctgcg gtgcggtgga gccgcgcttg
       61 accgtccatg cccgtacttc cttctcgccg gcggtgaaat aagtttgcag gccgagcagt
      121 ttgtacgcgg cgcggatcac acggttcaat cccggctcgt ccagacccat gtccttgaga
      181 aaggcatcgc ggtcctgatc gtccaattgc gagagttctt cctcgattgc ggcgcatacc
      241 ggcacgactt ccgcgccttc ggccgcggcg cgggcgcgca ccttgtccag gtacggattg
      301 ttcgcgaagc cgtcttcctt gacgttggcg atgtacatga gcggtttgag cgtaagcagg
      361 aacaggtcgc ggaccagcgc cttctcgtcg tcgtcgaggc ccagactgcg cgcggacttg
      421 ccttcgttga gtcccgtcgc gagcttctgc aacacgggcc tcttcgccaa cgcttccttg
      481 tcgttggcct tggccgcgcg ttccgcacga ttgagcgctt tgtccaccga ttccagatcg
      541 gccagcgcca gttcggtgtc gatcgtctcg atgtcggcga tcggatcgat cttgccggcg
      601 acgtggacga tgtccggatg ctcgaaacaa cgcacgacgt gcgcgatcgc gtcgacctcg
      661 cggatgtgcg cgaggaactt gttgccgagt ccttcgccct tcgaggcgcc ggcgacgagg
      721 ccggcgatgt cgacgaattc gaccgccgtg ggcaccagct tctgcggttt gacgatttcc
      781 gcgagctcgt tcaggcgcgg atcgggtacc ggcaccacac cgatattcgg atcgatcgtg
      841 caaaacggaa aattcgccgc ggcgattccc gccttggtga gcgcgttgaa aagcgtggac
      901 ttgccgacgt tcggcaggcc gacgatgccg catttgattc ccatttcgtt tttattcctg
      961 tgccgtgtgc aggcgcttca tcgcttcgtt gaaatcgccc gcgaccgcga gcggcaatac
     1021 gtcgagcgcc gcgccgaccg cgtcgagaat cgcatcctcg tccgccttgc cgggacgccc
     1081 gagcacccac ggcgtgaccc gatccttgtg gccgggatgg ccaatgccga tccgcagccg
     1141 atggaactcg ccatgtccga gatgcgcgaa gatgtcgcgc agtccgttct gcccgccatg
     1201 gccgccgtcg aacttcagcc gcgccgcgcc cggcggcaga tccagatcgt cgtgggcgac
     1261 gagcatttcc tccggcgcga tcttccagta gcgcagcgcg gacgccaccg cgatgccgct
     1321 cttgttcatg aatgtgttcg gcttgagcag gagcaacggc tggccatcga ggacgacttt
     1381 cgccgtctcg ccgtgcaatt tcgtctccag cccgaagcgc gcgccttcac gctgcgcaag
     1441 cgcgtcgacg aaccagaacc cggcgttgtg ccgggttcgc agatgttcgg ccccggggtt
     1501 gcccaggccg acgagtaggc gcaagccggc catcgcggca atatcgcaag aatccctccc
     1561 cgcctgcgcg gggaaggacg cgcgaaacgc ttacttcttc tcgtctttct tcgctgccgc
     1621 gggagccgcc gcgccacccg caggtgccgc gccggtggcg ccagccgcag gtgccgcgcc
     1681 agtagcgcca gccgcctcga cgaccggagc cacttcgact tcctgcttca tctcttgcgc
     1741 ggtgaccacg gccgtgtcgt gctccttgcc caggcgcaat tccggaattt ccacgccgtt
     1801 cggcagcttg agcgccgaca ggtggacgat gtcgccgacc ttgagttcgg acagatcgac
     1861 ttcgatgtgc tccggcagat ccttcggcag gcaggcgatt tccacgtcgt tca
//
LOCUS       DASRVO010000223         3786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_58470, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000223 DASRVO010000000
VERSION     DASRVO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58470"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VFF05_02690"
     CDS             <1..966
                     /locus_tag="VFF05_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZW50741.1"
                     /translation="YKLAVRKDPGRWFFLKYDTTQERHSQRLLEGALLLSVLGFLAVS
                     LVLGFWLSSRVMEPVTELAARVKAMERSGKAEPLAPHFANDEVGALAAAFDDYARKLT
                     ALVERDREFNADVSHELRTPLAVIATTTELLLNAENLTDKVRERLKRIERASRQSAEL
                     TNALLLLSRSERSPPADGETTDVAEVVEQVIDVYRAHLGKKPVAVRTDVEHAIEVVAP
                     ASVVAVALGNLIGNAFKYTQTGEVVVTVGSGKVMVEDTGPGIKPEDAERLFERGVRGE
                     GAGGKGAGLGLAIVRRLCELYDWRVTLAPRLQGGAVATLDFRLRA"
     gene            complement(967..1884)
                     /locus_tag="VFF05_02695"
     CDS             complement(967..1884)
                     /locus_tag="VFF05_02695"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HZW50742.1"
                     /translation="MAREYPPYIWHNGKIKPWAEATTHVMAHAVHYGSSVFEGIRSYA
                     TPKGQAIFRLSDHLKRLYHSAKIYDLPVPFAQDELAQASRDVIKANDLKKAYLRPVAF
                     RGLGGFGLSAECPTDVAVAAWDMGPYLGAGVLEAGIDACVSSWQRFAPNTIPAGAKAG
                     GNYLSGQLIAREARRLGFGEGIALASTGLLSEGAGENLFMVFDSALHTTPVSAALLNG
                     ITRNSLMTLARGAGIDVVERDIPREYLYLCDELFMCGTAAEITPIRSVDGKPVGTGKP
                     GPVTAKMQKLFFGLFDGSTPDKYGWLEYV"
     gene            1959..2699
                     /locus_tag="VFF05_02700"
     CDS             1959..2699
                     /locus_tag="VFF05_02700"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZW50743.1"
                     /translation="MSEHDIILSLAFALIALLYSSVGHAGASGYLAVMALAELAPAQM
                     KPIALIMNTAVAAIGTFNFWRGGWFRWRLFWPFALTSIPLALVGGAITLPDIYYKPLV
                     GAVLLYSGARLLFPPRGRETDLHPPAIPVAVGAGAGLGFLSGLTGVGGGIFLSPLVVL
                     LGWGNVREASGVAAPFILVNSIAGLIGRGLVTTAIPGPTVPWLAAVLVGGVVGSWLGS
                     RRLPMPSIRRLLAIVLFLAGAKMLFGLG"
     gene            complement(2710..3531)
                     /gene="metF"
                     /locus_tag="VFF05_02705"
     CDS             complement(2710..3531)
                     /gene="metF"
                     /locus_tag="VFF05_02705"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HZW50744.1"
                     /translation="MSPISLEFFPPKTDEQKATLEAALPKLKALKPDYVSVTFGAGGS
                     TLAYTPQIVRHLRDAHGLDAAPHLSCMGGTRAEIRQLLDEYKAMGCKRIVALRGDLPS
                     GMASYGDFRYASELVEFIRRECDGFFHIEVACYPEMHPQAGDAHADLKNFAAKVKAGA
                     NGAITQYFYNPDAYFRFVDEARKHGIEIPIVPGVMPISQYAQLRRFSDLCGAEIPRWI
                     AKRLEAYRDDVEAIREFGADVVADLCRKLIAGGAPGIHFYTLNRAKATLAVCERL"
     gene            complement(3637..>3786)
                     /locus_tag="VFF05_02710"
     CDS             complement(3637..>3786)
                     /locus_tag="VFF05_02710"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HZW50745.1"
                     /translation="ANNDAKLVVLSGEPIDEPIVGYGPFVMNTTDEIQQAMVDFNSGR
                     FGKMH"
BASE COUNT          586 a         1223 c         1263 g          714 t
ORIGIN      
        1 tacaagctcg ccgtgcgcaa ggatcccggg cgctggttct tcctcaagta cgacaccacg
       61 caggaacgcc acagccagcg tttgctcgaa ggcgcgctgc tcttgtcggt gctgggattc
      121 cttgccgttt cactggtgct cgggttctgg ctttcctcgc gcgtgatgga accggttacc
      181 gaattggcgg cacgcgtcaa ggcaatggaa cgtagcggca aggctgagcc gctggcgccg
      241 catttcgcca acgacgaggt cggcgcgctc gccgccgcct tcgacgatta cgcgcgcaag
      301 ctcaccgcgc tggtcgagcg cgaccgcgaa ttcaatgccg atgtcagcca cgagttgcgc
      361 acgccgctcg ccgtgatcgc cacgaccacc gagctgctgc tcaacgccga aaacctcacc
      421 gacaaggtgc gcgaacgctt gaagcgcatc gagcgcgcct cgcgccagtc cgccgaactc
      481 accaatgcgc tgttgctgct ctcgcgcagc gaacgctcgc cgcccgccga tggcgaaacc
      541 accgacgttg ccgaggtggt cgagcaggtc atcgatgtct accgtgccca cctcggcaag
      601 aaaccggtcg cggtcaggac ggacgtcgag cacgcaatcg aagtggtcgc gccggcttcg
      661 gtcgtcgcgg tcgcgctcgg caacctgatc ggcaatgcgt tcaagtacac gcagaccggc
      721 gaagtcgtgg tcacggtcgg ttccgggaaa gtcatggtcg aagacaccgg tcccggcatc
      781 aagcccgagg acgccgagcg cctgttcgaa cgcggcgtgc gcggcgaggg cgccggcggc
      841 aagggcgcgg ggctcggcct cgccatcgtg cgccgcctgt gcgaactcta cgactggcgc
      901 gtgacgctcg cgccgcggct gcagggtggg gcggtggcga cgctggattt caggctgcgc
      961 gcctgatcac acgtattcca gccaaccgta tttgtccggc gtcgagccgt cgaacaggcc
     1021 gaagaacagc ttctgcatct tcgccgtgac cgggcccggc ttgcccgtgc cgaccggctt
     1081 gccgtccacc gaacggatcg gtgtgatctc ggccgcggtg ccgcacatga agagctcgtc
     1141 gcacaaatac aggtattcgc gagggatgtc gcgctcgacg acgtcgatgc cggcgccgcg
     1201 cgccagcgtc atcagcgaat tgcgcgtgat gccgttcagt aacgcggcgc tgaccggcgt
     1261 cgtatgcagc gcactgtcga agaccatgaa caggttttcg ccggcgcctt cgctgagcag
     1321 gccggtcgaa gccagcgcga tgccttcgcc gaaacccagg cgccgcgctt cgcgcgcgat
     1381 gagctggccg gaaagataat tgccgccggc cttggcgccg gccggaatcg tgttcggcgc
     1441 aaagcgctgc cagctcgaca cgcaggcgtc gatgcccgct tcgagcacgc cggcacccag
     1501 atacggaccc atgtcccacg ccgccacggc gacgtcggtc gggcattcgg cggacaagcc
     1561 aaagccgccg aggccgcgaa acgccactgg tcgcagatac gccttcttca gatcgttcgc
     1621 cttgatcacg tcgcggctgg cctgcgcgag ttcgtcctgc gcgaacggta ccggcagatc
     1681 gtatattttc gcggagtgat acaagcgctt gaggtgatcg ctcaggcgga aaatcgcctg
     1741 gcccttgggc gtcgcatagc tgcggatgcc ctcgaacacc gacgagccgt aatgcaccgc
     1801 gtgcgccatc acgtgcgtcg tggcctcggc ccagggtttg atcttgccgt tgtgccagat
     1861 gtacggcgga tactctcggg ccatggtgat tctccgtgcg gcagtgcagt tgccaattat
     1921 ggcatgctcg ccgctttcgg gattcgggaa tcttgcgcat gagcgagcac gacatcatcc
     1981 tgtccctggc attcgcactg atcgcgctgc tgtattccag cgtcggccat gctggcgcct
     2041 cgggttacct tgcggtgatg gccttggcgg agcttgcccc ggcgcagatg aagccgattg
     2101 cgctgatcat gaacacggcg gtggccgcga tcggcacgtt caatttctgg cgcggcggct
     2161 ggttccgctg gcgcctgttc tggccgtttg cgctgacctc gattccgctc gcgctcgtcg
     2221 gcggtgcgat cacgctgccg gacatctact acaagccgtt ggtcggcgcg gtgttgctgt
     2281 attccggcgc acgcctgctg tttccgccgc gtggacgcga gacggatttg catccgccgg
     2341 cgattccggt cgcggtcggc gcgggcgcgg ggctgggatt cctgtccggc ctcaccggcg
     2401 tcggcggcgg gattttcctg agcccgctcg tcgtgctgct cggctggggc aacgtgcgcg
     2461 aggcctcggg cgtggccgcg ccgttcatcc tcgtcaattc catcgccggc ctgatcgggc
     2521 gtgggctcgt caccaccgcg attcccggtc cgaccgtgcc gtggctcgcc gccgtgctgg
     2581 ttggcggcgt ggtgggctcg tggcttggca gccggcgcct gccgatgccg tcgatccggc
     2641 gtctgcttgc gatcgtgctg tttctggccg gcgccaagat gctgttcggc ctgggttaaa
     2701 cggcttgagt taaagccgct cgcaaaccgc cagtgtcgcc ttggcgcggt tcaatgtata
     2761 aaagtggatt cccggcgctc cgccggcgat gagcttgcgg cacaggtcgg cgaccacgtc
     2821 ggcgccgaat tcgcggatcg cctcgacgtc gtcgcgatag gcctcgaggc gcttggcgat
     2881 ccagcgcggg atttccgcgc cgcacaggtc cgagaaccgg cgcagctgcg cgtattgcga
     2941 gatcggcatg acacccggca cgatgggaat ttcgatgccg tgcttgcgcg cctcgtcgac
     3001 gaaacgaaag taggcatccg ggttgtagaa atactgcgtg atcgcgccgt tcgcgccggc
     3061 cttcaccttg gccgcaaaat tcttcaggtc ggcgtgcgca tcgccggcct gtggatgcat
     3121 ttccggatag caggcgacct cgatgtggaa gaaaccgtcg cattcgcggc ggatgaattc
     3181 cacgagttcg ctggcgtagc gaaagtcgcc gtagctggcc atgcccgagg gcaggtcgcc
     3241 gcgcagcgcg acgatgcgct tgcagcccat cgccttgtac tcgtcgagca gttggcggat
     3301 ttccgcgcgc gtgccgccca tgcacgacag gtgcggcgcg gcgtccaggc cgtgcgcatc
     3361 gcgcaggtgg cgcacgatct gcggcgtgta ggccagcgtc gagccgcccg cgccgaaggt
     3421 gacgctgacg tagtcgggct tgagcgcctt cagtttcggc agcgccgctt cgagcgtggc
     3481 cttctgctcg tcggttttcg gcggaaagaa ttcgaggctg atcggcgaca tcgcgggcaa
     3541 tgggcagcag gggttgctgc agtatatctc ttcgtcgcga tgaagcgata ttcgaacggg
     3601 actccggcgc gcgaccgcac gccggagttt cgcaggtcag tgcatcttgc cgaaacgtcc
     3661 gctgttgaaa tccaccatcg cctgctggat ttcgtccgtc gtgttcatca cgaacgggcc
     3721 gtagccgacg atgggttcgt caatgggttc accgctgagc acgaccagct tggcgtcgtt
     3781 gttcgc
//
LOCUS       DASRVO010000224         5096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_266435, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000224 DASRVO010000000
VERSION     DASRVO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266435"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VFF05_02715"
     CDS             <1..655
                     /locus_tag="VFF05_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HZW50746.1"
                     /translation="MRWMKKDMGGAAHAIALADMIMRARLPVRLTLLVPAVENAVGGD
                     AYRPGEVIRTRAGLTVEVDNTDAEGRLVLCDALAYAVEKKPDLIVDFATLTGAARVAL
                     GPDLPALFANRDDLAASTHEAGVREADPLWRLPLWQPYARMLESSIADIANAGPSRHA
                     GAITAALYLQRFVPERQAWMHVDVYAWNDSDRPGRPKGGEAQSLRAFFTMLKERYSK"
     gene            complement(659..1006)
                     /locus_tag="VFF05_02720"
     CDS             complement(659..1006)
                     /locus_tag="VFF05_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50747.1"
                     /translation="MLSAHTPHNSFAEAGFNGIDNTRRADVDSTSPVVTLDKTKAAPK
                     PAADPRQDDGLQADERSIKDELADEKARKAEKDVVLHEVANILADEVNLLRTDTKLAA
                     RVLPHSAIDKPVD"
     gene            complement(1091..2974)
                     /locus_tag="VFF05_02725"
     CDS             complement(1091..2974)
                     /locus_tag="VFF05_02725"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HZW50748.1"
                     /translation="MLKSSALRHLAWIALVFAAATTAKPAPAPHDVVLKPTSEQAPAA
                     LLATRFLTRFHYKAEPLDAAMSQKIFDRYFDSLDGDRLFFVQADVDRFMPERDKLGDA
                     IYDENLSVPFAIFNLYEQRVTERTQYARDLLKKTFDFDKTETYTYQRDKAPWAKSTAE
                     LDDLWRKRVKNDWLRLKLAGQDAAKIRETLDKRYANYLDRIRQIDSEDVFQTFMNAYA
                     LSIDPHTNYLGPRASENFDIAMKLSLEGIGAVLQRDDDYTAIREIVAGGPAAMSGKFK
                     VGDRIVGVGQGANSQIVDVVGWRLDDVVDLIRGPKDTTVRLEVLPADAGPDGKHELIA
                     LVRKKVNIEEQAAKSSVIEIKDGDATRRVGVISLPTFYEDFDARRRGDKNYKSATRDV
                     AKLLDGLKAQHVDAVLMDLRNNGGGSLSEAIDLTGLFVGKGPVVQVRNADGRVEVGRN
                     THQNMVWSGPFAVLVNRNSASASEIFAGAIQDYGRGLIVGEQTYGKGTVQNLVDLDQM
                     SQSEKPAFGELKMTIQQFFRVDGASTQLRGVTPDIAFPVTGDFAQNGESTNKNALAWA
                     SIAAADYTPTSDLQSILPMLKSMHDARVAKEKPWLAYEADVADFRKLRAETTISLNEK
                     VRR"
     gene            complement(2997..3983)
                     /gene="lipA"
                     /locus_tag="VFF05_02730"
     CDS             complement(2997..3983)
                     /gene="lipA"
                     /locus_tag="VFF05_02730"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZW50749.1"
                     /translation="MSVDKTIPITVVAEGAKLLGEDKIARNRADFDTAVPILRKPDWI
                     RVRIPPGNAVARLKAKLRANRLVTVCEEASCPNIHECFGKGTATFMILGEVCTRRCSF
                     CDVAHGRPKPPDADEPRHLAETIRDMGLRYVVVTSVDRDDLRDGGAAHFAACIREVRA
                     ANPDIHIEILTPDFRGKGRMERALEVLAENPPDVFNHNLETVRDLYPNVRPGADYDWS
                     LTLLQRFKAQHPSIPTKSGIMLGLGETAEQVHGALNDLRAHAVDMVTIGQYLQPTPHH
                     HPVLRYWTPAEFDELADYGQRLGFQHVASGPLVRSSYHADRMAHAAGYVEVA"
     gene            complement(3980..4669)
                     /gene="lipB"
                     /locus_tag="VFF05_02735"
     CDS             complement(3980..4669)
                     /gene="lipB"
                     /locus_tag="VFF05_02735"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZW50750.1"
                     /translation="MAELLIRRMPGLSPYEPVWRAMQAFTDVRTPQTPDELWVVEHEP
                     VFTLGQAGKWEHVLDPGTIPVIPVDRGGQVTYHGPGQIVAYPLIDLRRAGIGVREYVQ
                     RIEQAVIDTLAEWTIVALRRDGAPGVYVNDAKIAALGIRVRRGCTFHGLAFNVDMDLE
                     PFHRINPCGFQGLQVTQMLDLGGPSRLADVETVLVAELARQFGLQAIPADPILTSGAG
                     TPCASRVNVTA"
     gene            complement(4674..4964)
                     /locus_tag="VFF05_02740"
     CDS             complement(4674..4964)
                     /locus_tag="VFF05_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HZW50751.1"
                     /translation="MKSLDDIAPRNPQQGFQFPGVFEITAMGNARAQLEDEVQRIIRD
                     AGLEVLAEAVRVRPSREGNYVSVTASFMCPTREKYDEIHAALRAHPDVHWTL"
BASE COUNT          814 a         1605 c         1698 g          979 t
ORIGIN      
        1 catgcgctgg atgaagaagg acatgggcgg tgcggcgcac gcgatcgcgc tggccgacat
       61 gatcatgcgc gcgcgcctgc cggtgcgcct gaccctgctc gtgccggcgg tcgagaacgc
      121 ggtcggcggc gatgcgtacc gtcccggcga agtgatccgc acgcgcgccg ggttgaccgt
      181 cgaggtcgac aacaccgacg ccgaaggccg cctggtcctg tgcgacgcgc tggcttatgc
      241 ggtcgaaaag aagcccgacc tgatcgtgga tttcgccacc ctgaccggcg ccgcacgcgt
      301 ggcgttgggt ccggatctgc cagccctgtt cgccaatcgt gacgatctgg ctgccagcac
      361 gcacgaagcc ggtgtacgcg aggccgatcc gctgtggcgc ctgccgttgt ggcagcctta
      421 tgcgcgcatg ctcgaatcgt ccatcgccga catcgccaat gccgggcctt cgcgccatgc
      481 cggcgcgatc accgcagcgc tgtacctgca gcgattcgtg cctgagcgcc aggcctggat
      541 gcatgtggat gtctacgcct ggaacgattc cgaccgcccc ggtcggccga aaggcggcga
      601 agcgcaaagt ctgcgcgcgt ttttcacgat gttgaaagag cgctattcga agtagcgctc
      661 aatccaccgg cttgtcgatc gccgaatgcg gcagcacgcg cgcggcgagc ttggtgtccg
      721 tgcgcagcag gttcacttcg tcggcgagga tgttcgccac ctcgtgcagg acgacatcct
      781 tctcggcctt gcgcgctttt tcgtcggcaa gctcgtcctt gatgctgcgt tcgtctgcct
      841 gcaggccgtc gtcctggcgc ggatcggccg cgggtttcgg cgccgccttg gtcttgtcca
      901 gcgtgacaac cggactcgtc gagtccacgt cggcacgtcg ggtattgtca attccgttga
      961 agccggcttc cgcgaagctg ttgtgtgggg tatgtgcgga gagcatcttg tgcacatcgc
     1021 caaccggact tttcgcctgc gcaaggtccg ggtgccgctc gttgcgcgtc ttttcctgct
     1081 cgtcgcgttc ttagcggcgc actttttcgt tcaatgaaat cgtcgtttcg gcgcgcagct
     1141 tgcggaaatc cgccacgtcc gcttcgtagg ccagccacgg cttctccttg gccacgcgtg
     1201 catcgtgcat ggacttgagc atcggcagga tggattgcag gtccgacgtc ggggtgtagt
     1261 cggcggccgc gatcgacgcc catgccaacg cattcttgtt cgtcgactcg ccattctggg
     1321 cgaaatcgcc ggtgaccgga aacgcgatgt cgggcgtgac gccgcgcaac tgcgtcgagg
     1381 cgccgtcgac gcggaagaat tgctggatcg tcatcttcag ttcgccgaac gcaggctttt
     1441 cgctctgcga catctggtcg aggtcgacca ggttctgcac cgtgcccttg ccgtaggtct
     1501 gttcgcccac gatcaggcca cggccgtaat cctggatcgc gccagcgaag atttccgacg
     1561 ccgaggccga attgcggttg accagcaccg cgaacgggcc gctccagacc atgttctggt
     1621 gcgtattgcg gccgacttcg acgcggccat cggcgttgcg cacctgcacg accggaccct
     1681 tgccgacgaa cagtccggtc aggtcgatcg cctcggacaa tgagccgccg ccgttgttgc
     1741 gcaagtccat gagcacggcg tcgacatgct gcgccttcag gccgtcgagc agcttggcga
     1801 cgtcgcgcgt ggcgctcttg tagttcttgt cgccgcgccg gcgcgcgtcg aaatcttcgt
     1861 aaaaagtcgg caggctgatc acgcccacgc ggcgcgtggc gtcgccgtcc ttgatctcga
     1921 tgacggagct tttcgccgcc tgttcctcga tgttgacctt cttgcgcacc agcgcgatga
     1981 gctcgtgctt gccatcgggg ccggcgtcgg cgggcagcac ttccaggcgc accgtggtgt
     2041 ccttggggcc gcgaatcagg tcgaccacgt cgtccaggcg ccagccgacc acgtcgacga
     2101 tctggctgtt cgcgccctga ccgacgccga cgatgcggtc gccaaccttg aacttgcccg
     2161 acatcgccgc cgggccgccg gcgacgatct cacggatcgc cgtgtagtcg tcgtcgcgtt
     2221 gcaataccgc gccgatgcct tcgagcgaaa gcttcatcgc gatgtcgaaa ttttcgctgg
     2281 cgcgcgggcc gaggtaattc gtgtgcggat cgatcgacag cgcgtacgcg ttcatgaagg
     2341 tctggaacac gtcctccgaa tcgatctggc ggatgcggtc gaggtagttg gcgtagcgct
     2401 tgtccagcgt ttcacggatt ttcgcggcgt cttgccccgc gagcttgagg cgcagccagt
     2461 cgttcttcac gcgcttgcgc cacaggtcgt cgagttcggc ggtggatttt gcccacggtg
     2521 ccttgtcgcg ctgatacgtg taggtctcgg tcttgtcgaa atcgaaagtc ttcttgagca
     2581 gatcgcgcgc gtattgcgtc cgctcggtga cgcgctgctc gtacaggttg aagatcgcga
     2641 acggcaccga caggttttcg tcgtagatgg cgtcgccgag tttgtcgcgc tccggcatga
     2701 agcggtcgac atcggcctgc acgaagaaca ggcggtcgcc atcgagcgaa tcgaaataac
     2761 ggtcgaagat tttctgcgac attgccgcgt cgagcggctc ggccttgtag tgaaagcgag
     2821 tcaggaaacg cgtcgccagc agtgctgccg gcgcctgctc ggaggtcggc ttgagcacca
     2881 cgtcgtgcgg cgccggcgcc ggtttggcgg tagtggccgc ggcaaatacg agggcgatcc
     2941 aggccaggtg gcgaagggcg gatgatttga gcatgacagc agggtttccg gaaaaattag
     3001 gcgacttcaa cgtaaccggc cgcgtgggcc atgcggtcgg cgtgatacga actgcgcacc
     3061 agcggccccg aagcgacgtg ctggaagcca aggcgctggc cgtaatcggc aagctcgtcg
     3121 aattccgcgg gcgtccagta gcgcagcacc ggatggtgat gcggcgtggg ttgcaggtat
     3181 tgtccgatcg tgaccatatc gaccgcgtgt gcgcgcagat cgttcaacgc gccgtgcacc
     3241 tgttcggcgg tttcgcccag gccgagcatg atgccggact tggtcgggat gctgggatgc
     3301 tgcgccttga agcgctgcag cagggtcagc gaccagtcgt aatcggcgcc cggacgcacg
     3361 ttcgggtaca ggtcgcgcac ggtttcgagg ttgtggttga acacatcggg cggattttcg
     3421 gcgagcactt ccagcgcacg ctccatgcgg cccttgccgc ggaaatccgg ggtcaggatc
     3481 tcgatgtgga tgtcgggatt ggccgcacgc acttcgcgga tgcaggcggc gaaatgcgcc
     3541 gcgccgccgt cgcgcagatc gtcgcggtcg accgaggtga cgacgacata acgcaggccc
     3601 atgtcgcgga tggtttcggc caggtgccgc ggttcgtcgg catccggcgg cttgggccgg
     3661 ccgtgggcga cgtcgcaaaa cgagcagcgc cgcgtgcaca cttcgccgag tatcatgaac
     3721 gtggccgtgc ctttgccgaa gcattcgtgg atgttcgggc acgacgcttc ttcgcacacg
     3781 gtgacgaggc ggttcgcgcg cagcttggcc ttcaggcgcg ccaccgcgtt gccgggcgga
     3841 atgcgcacgc ggatccagtc cggtttgcgc agaatcggta cagccgtatc gaagtcggcg
     3901 cgattgcgcg cgatcttgtc ctcgccgagc agcttggcac cttcggcaac gacggtaatc
     3961 ggaatggtct tgtccacgct catgcagtca cgttaacgcg gctcgcgcag ggggtgccag
     4021 cgccagaagt caggatgggg tcggccggaa tcgcttgcaa cccgaactgg cgcgccaatt
     4081 cggccaccag cacggtttcc acgtcggcca gccgcgacgg gccacccaag tctagcatct
     4141 gggtgacctg caaaccctga aagccgcagg gattgatgcg atggaagggt tccaggtcca
     4201 tgtccacatt gaaggccagg ccgtggaacg tgcagccgcg acgcacgcgg atgccgagcg
     4261 cagcgatctt ggcgtcgttc acgtagacgc cgggcgcacc gtcgcggcgc agcgcgacga
     4321 tagtccactc ggccagcgtg tcgatcaccg cctgctcgat gcgctgcaca tactcgcgca
     4381 cgccgatgcc ggcgcggcgc aggtcgatga gcggataggc cacgatctgg cccggcccgt
     4441 ggtaggtcac ctggccgccg cggtcgaccg gaatcaccgg gatcgtgccc ggatcgagca
     4501 catgttccca cttgccggcc tggccaagcg tgaacaccgg ctcgtgttcg accacccaca
     4561 attcgtccgg tgtctgcggc gtgcgcacat ccgtgaacgc ctgcatcgcc cgccacaccg
     4621 gttcgtaggg cgacaggccg ggcatgcggc ggatgagcag ttcggccacg gcgttacaac
     4681 gtccagtgca cgtccgggtg ggcgcgcagc gcggcgtgga tttcgtcgta tttctcgcgc
     4741 gtcgggcaca tgaagctcgc ggtcacagat acgtagttgc cctcgcgcga cggccgcacg
     4801 cgcacggctt cggccagcac ctcgaggccg gcgtcgcgga tgatgcgctg gacttcgtcc
     4861 tcgagttgcg cgcgcgcgtt gcccatcgcg gtgatctcga acacaccggg aaactggaag
     4921 ccctgttgcg gattccgcgg ggcgatgtcg tcgagcgatt tcatgcggca agcgtggcca
     4981 aggcgttctt attgcgtatt cggcgtcggg gaaatttcca ccttctgatc gcccttgaac
     5041 cacagccaga tgccgtcgga caggcgtccg aagaaaccgg cttccggcgc atcggc
//
LOCUS       DASRVO010000225         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_6829, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000225 DASRVO010000000
VERSION     DASRVO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6829"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /gene="trpS"
                     /locus_tag="VFF05_02745"
     CDS             <1..1041
                     /gene="trpS"
                     /locus_tag="VFF05_02745"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZW50752.1"
                     /translation="RSTLEIAASWLACGLDPGKVYFYRQSDIAEIPELTWMLTCVTAK
                     GLLNRAHAYKAATDANRDKGEDADAGINAGLYMYPVLMAADILVFNANQVPVGRDQVQ
                     HIEMARDIGQRFNHIYGGEFFALPEAAIEENVATLPGTDGRKMSKSYDNTIPLWLPEK
                     DLRKAILGVVTNSQAPGEAKNPDESNLFAIYQAFASADETAAMRRAYADGIGWGDAKQ
                     QVFERVNAELAPLREKYAALVADPAQIEDRLRAGAARARRLATPFVARLREAVGLRSL
                     SQSSAAPEPEPQKKARVEKPNLQPVVFRDGETFAFKVLDAKKTILAEKGGFADPKAAM
                     AAAREVMSKLAS"
BASE COUNT          211 a          339 c          342 g          158 t
ORIGIN      
        1 cgctcgaccc tggaaatcgc cgcaagctgg ctcgcctgcg gcctggatcc gggaaaagta
       61 tacttctacc gccagtcgga cattgccgaa attcccgagc tgacctggat gctgacctgc
      121 gtcaccgcca agggcctgct caaccgtgcc cacgcgtaca aggccgcgac cgacgccaac
      181 cgcgacaagg gcgaggacgc cgacgccggc atcaacgccg gcctgtacat gtacccggtg
      241 ctgatggcgg ccgacatcct ggtgttcaac gcgaaccagg tcccggtcgg ccgcgaccag
      301 gtccagcaca tcgagatggc gcgcgacatc ggccagcgct tcaaccacat ctacggcggc
      361 gaattcttcg ccttgcccga ggcggcaatc gaggaaaacg tcgcgacgct gcccggcacc
      421 gacgggcgca agatgtcgaa gagttacgac aacacgattc cgctgtggct gccggaaaag
      481 gatttgcgca aggcgattct cggcgtcgtt accaattcgc aggcaccggg cgaggcgaag
      541 aatccggacg agtcaaacct gttcgcgatc tatcaggcat tcgccagcgc cgacgaaacc
      601 gccgcgatgc gccgcgccta cgccgacggc atcggctggg gcgacgccaa gcagcaggtc
      661 ttcgagcgcg tgaacgcgga actcgcgccg ctgcgcgaga aatacgctgc gctggtcgcc
      721 gatccggcgc agatcgaaga tcgccttcgc gcgggcgcag cgcgcgcgcg aaggctggcg
      781 acgccgttcg ttgcgcggtt gcgcgaggcg gtgggattgc gttcgttgag tcagtcgtcg
      841 gcagcgccag agccagagcc acagaaaaaa gcgcgggtgg agaaacccaa cctgcaaccc
      901 gtcgttttcc gcgatggcga gactttcgcg ttcaaggtgc tggatgcgaa aaaaaccatc
      961 ctcgccgaga aaggcgggtt cgccgatccc aaggccgcca tggcggccgc gcgcgaggtg
     1021 atgtcgaaac ttgcttcttg agtcgggggc
//
LOCUS       DASRVO010000226         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_136354, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000226 DASRVO010000000
VERSION     DASRVO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136354"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFF05_02750"
     CDS             complement(<1..190)
                     /locus_tag="VFF05_02750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HZW50753.1"
                     /translation="MATIVILGAGIGGMSLAYELRQRLGGGHRITVIGDGPRFSFTPS
                     NPWVAVGWRKGVDIGIEAA"
     gene            327..686
                     /locus_tag="VFF05_02755"
     CDS             327..686
                     /locus_tag="VFF05_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZW50754.1"
                     /translation="MTSIKSRQIGVNAAAMTMAAHADAASGLLKSLANAQRLRILCLL
                     AGGERNVGELNDLLDLSQSALSQHLARLREDGLVATRREAQTIFYRLASGPAKRLIKT
                     LHGIYCAAPQPRANVAR"
     gene            complement(690..1571)
                     /locus_tag="VFF05_02760"
     CDS             complement(690..1571)
                     /locus_tag="VFF05_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZW50755.1"
                     /translation="MGDHASPLVESFFDKDTCTFTHVVYDRDGGECAIVDPVLDYDAA
                     AARTSTASADAVAAFVRQHDLRTTWILETHAHADHLSAGAYLRDALGAKLAIGRGIVG
                     VQAHFKNVFGLGDEFVADGGQFDRLFDDGDVFAIGALQARVMATPGHTADSLTYLVGG
                     AAFIGDTLFAPETGSARADFPGGDAARLFASIRNILSLPPDTRLFLCHDYPPSTRAPV
                     AQTSIAAQRENNPHIRDAATEADYIALRRARDATLGAPRLLLPALQVNIRGGRLPEPD
                     ANGIRYLKVPLNRIGRE"
     gene            complement(1616..>2060)
                     /gene="phoU"
                     /locus_tag="VFF05_02765"
     CDS             complement(1616..>2060)
                     /gene="phoU"
                     /locus_tag="VFF05_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZW50756.1"
                     /translation="ASDIERIGDYAANVAKRSIPLSMVAPVAPARGLRRLSDLAVAAV
                     RKVLDAYRNRDAEKAHEVWNEDVELDDAYTGLFRELLTYMMEDPRSITSCTHLLFMAK
                     NIERIGDHATNIAESVWFAVTGKPLQTPRRKGDVTAEPVMPKLGG"
BASE COUNT          365 a          669 c          672 g          354 t
ORIGIN      
        1 gcgcggcctc gatgccgatg tccacccctt tgcgccagcc gaccgccacc cacggattcg
       61 acggcgtgaa cgaaaaccgc ggcccgtcgc cgatgacggt gatgcggtgg ccgccgccaa
      121 gccgttggcg cagctcgtat gccaagctca tgccgccgat gccggcgccc aatatcacga
      181 tcgttgccat gccatcgccc tctgttgtgc cttggatccg ttcagaatga tcgcttgcta
      241 atttagcatc tactaaatta gaattcaagc gaacgatatg cgactatcca gcaacacgat
      301 gatccgccag gcgcggggaa ggcaccatga catcgatcaa atccagacaa atcggggtaa
      361 acgccgcggc catgacgatg gccgcgcacg cggatgccgc ctccggattg ctcaagtccc
      421 tagcgaacgc gcagcgtctg cgcatcctgt gccttctggc cggcggcgaa cgcaacgtcg
      481 gcgagctgaa cgacttgctc gatctgagcc aatcggcact gtcgcaacac ctggcgcggc
      541 tgcgtgaaga cggcctggtg gcgacgcgac gcgaggcaca gaccatcttt taccggctcg
      601 caagcggacc ggcgaagcgc ctgatcaaga ccctgcacgg aatctattgc gcggcgccgc
      661 agccacgcgc gaatgtcgcg cgttgaggat tattcccgcc cgatccggtt cagcggaacc
      721 ttcagatagc ggattccgtt tgcgtccggc tcgggcagcc gcccgccgcg gatattgacc
      781 tgcaaggcgg gcagcagcag tcgcggcgcg ccgagcgtgg catcgcgtgc gcggcgcagc
      841 gcgatgtaat cggcctcggt cgccgcgtcg cggatgtgcg ggttgttttc gcgttgcgcg
      901 gcgatggagg tttgcgccac cggcgcgcgc gtgctcggcg ggtagtcgtg gcacaggaac
      961 aggcgtgtgt ccggcggcaa ggacaggata ttccggatgg aagcgaacag ccgcgccgcg
     1021 tcgccgccgg gaaaatccgc gcgtgcgctg cccgtttccg gcgcgaacag cgtatcgccg
     1081 atgaaggccg cgccgccgac gagataagtc aggctatccg ccgtgtgccc gggcgtggcc
     1141 atgacgcgag cttgcagcgc gccaatggcg aagacatcgc catcgtcgaa cagccgatcg
     1201 aattgaccgc cgtcggcgac gaactcgtcg cccaatccga acacgttctt gaagtgcgcc
     1261 tgcacgccga cgatgccacg gccgatggca agcttcgccc ccagcgcatc gcgcagatac
     1321 gcacccgcgc tcaggtgatc ggcgtgcgcg tgggtttcca ggatccacgt cgtgcgcaga
     1381 tcgtgctggc gcacgaaggc ggcgaccgca tcggcggaag ccgtcgacgt gcgtgccgcg
     1441 gccgcgtcgt agtcgaggac agggtcgaca atcgcgcatt cgccaccgtc acggtcatag
     1501 acgacatgtg tgaacgtgca tgtgtccttg tcgaaaaagc tttcgaccag cggtgatgcg
     1561 tgatcgccca tgcgaggctc cggtcccaag cggagcattg tgccaagaat cggcctcagc
     1621 cgcccagctt cggcatcacc ggctcggccg tgacatcgcc cttgcggcgc ggcgtctgca
     1681 acggcttgcc ggtgaccgcg aaccagacgc tttcggcgat gttggtggcg tggtcgccga
     1741 tgcgttcgat gttcttggcc atgaacagca ggtgcgtgca cgaggtgatg ctgcgcgggt
     1801 cttccatcat gtaggtgagc aattcgcgga acaggcccgt gtaggcgtcg tccagttcca
     1861 catcctcgtt ccagacttcg tgcgcctttt cggcgtcgcg gttgcggtag gcgtcgagca
     1921 ccttgcgcac cgccgcgacg gcgagatccg acaagcggcg caatccgcgc gccggcgcca
     1981 ccggcgccac catcgacaat ggaatggaac gcttggccac gttcgcggcg tagtcgccga
     2041 tgcgttcgat gtccgaagcg
//
LOCUS       DASRVO010000227         1251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_293136, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000227 DASRVO010000000
VERSION     DASRVO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293136"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFF05_02770"
     CDS             complement(<1..651)
                     /locus_tag="VFF05_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50757.1"
                     /translation="MADDDQLRMQAVVVDGFTGPLKNLRDSLRNAGQVKTGQAMEKDW
                     KSVGERVHGVTREINTALAPALRGIGISSLGVGAAVGGIFLGLKSSAQAGRNLKFFAD
                     QLRMSVKDLRELIALGQHFDIPEQATESALKAFANNMVMLKRHWGETYNALRAMNLGN
                     LAEDLINAPTLKDAFEKALEDLRKIPDPVYRRQIAQMLLGSDQWSIVAASAQSDADT"
     gene            complement(802..>1251)
                     /locus_tag="VFF05_02775"
     CDS             complement(802..>1251)
                     /locus_tag="VFF05_02775"
                     /inference="COORDINATES: protein motif:HMM:NF021614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HZW50758.1"
                     /translation="RLMTKPSTRVPAAAGEAEGEAILALARLFDLDRLGEAAVKRGEA
                     LADFRDKLLDALAIAREPIQWPYDFELSKSAEAHGETVTMLTLRAPTASELLAAGLFD
                     ADVSGPQLLDLIATLAGKAPALIRALPGLEMLRLTTKLSRVFRQAAS"
BASE COUNT          219 a          416 c          392 g          224 t
ORIGIN      
        1 tgtatcggcg tcagattgcg cagatgctgc cacgatcgac cattgatccg accccagcag
       61 catctgcgca atctgacgcc gatacaccgg gtcagggatc ttgcgcagat cctcgagcgc
      121 cttctcgaaa gcatccttca gcgtcggcgc gttgatcagg tcctcggcca agttgccgag
      181 attcatcgcg cgcagtgcgt tatatgtctc gccccaatgg cgcttcagca tcaccatgtt
      241 gttggcgaac gccttcaatg ccgattcggt tgcctgttcc ggaatgtcga aatgctgccc
      301 gagcgcgatc agctcgcgca aatctttcac cgacatacgc agttggtcgg cgaaaaactt
      361 gagattccgg ccggcctgcg cgctgctctt gagcccgagg aatatgccgc cgaccgcggc
      421 gccgacgccg agcgagctga tgccaatgcc gcgcagcgcc ggcgccagcg ccgtgttgat
      481 ctcgcgcgta acaccatgca cccgctctcc gaccgatttc caatccttct ccatcgcctg
      541 cccggtcttg acctggccgg cattgcgcaa cgaatcgcgc aaattcttga gcggtccggt
      601 gaagccgtcg acgacgacgg cctgcatgcg tagctggtcg tcatccgcca tggcgctgct
      661 ccgcaagagg attgccggtc agcatgcgcg tgcgcgcaag caggaaggcg cgggcgttcg
      721 gcccgaggtc ggcgggcagt tgccgcagcg gccagcaatg atagacgagg ccgagttcga
      781 gcgcctcgtc gatcgtttgg atcagctggc cgcctgtcga aaaacccggc taagcttggt
      841 ggtcagccgc aacatttcca gaccgggcag agcgcggatc aaggccggcg ccttgcccgc
      901 gagcgtcgcg atcagatcca gcagctgcgg accggagaca tcggcgtcga acagaccggc
      961 ggccagcaat tcactcgccg tcggcgcccg cagcgtcagc atggtgacgg tctcgccgtg
     1021 cgcctccgcc gacttggaga gctcgaaatc atagggccac tgaatcggct cgcgcgcgat
     1081 cgccagcgcg tcgagcagct tgtcgcggaa atcggccagc gcctcgccgc gcttgacggc
     1141 ggcttcgccg agtcgatcca gatcgaaaag ccgggcgagc gcgaggatcg cttcgccttc
     1201 ggcttcgccg gccgcggccg gaacgcgcgt gctcggtttc gtcatcaggc g
//
LOCUS       DASRVO010000228         1918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_293232, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000228 DASRVO010000000
VERSION     DASRVO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293232"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1918)
                     /gene="uvrA"
                     /locus_tag="VFF05_02780"
     CDS             complement(<1..>1918)
                     /gene="uvrA"
                     /locus_tag="VFF05_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZW50759.1"
                     /translation="GTCDGLGVTQVFDAARVVVHPNLSLAAGAVRGWDRRNMYYFQMI
                     LSLARHYKFDIDTPWEQLPEGIRHAVLYGSGDEQIAFRYLTESGGNVTRKHRFEGIVP
                     NLERRYRETESPAVREELSKFISERPCPDCHGERLNRSARNVFVGESNLPVLTGLPID
                     QALEFFHALTLPGWRGEIATKIVKEIRERLRFLVDVGLDYLTLDRQADSLSGGEAQRI
                     RLASQIGAGLVGVMYVLDEPSIGLHQRDNERLLGTLTRLRDLGNTVIVVEHDEDAIRA
                     ADHVVDIGPGAGVHGGEIVAQGKLKDVLSAKRSQTGDFLSGRRSIPVPRKRRAPDPQL
                     MLRVSGASGNNLKDVDLEIPAGLFVCVTGVSGSGKSTLINDTLFRHAAVELNGANEKP
                     APFRKIEGMEHFDKVVDIDQSPIGRTPRSNPATYTGLFTPLRELFAQVPEARARGYQP
                     GRFSFNVKGGRCEACEGDGLIKVEMHFLPDVYVPCDVCHGKRYNRETLEILYKGHTIS
                     DVLEMTVEDALKLFENIPALARKLETLMDVGLSYIKLGQSATTLSGGEAQRVKLSREL
                     SKRDTGRTLYILDEPTTGLHFHDIEQLLRVLHRLRDDGNTVVVIEHNLDVIKTADWIV
                     DLGPEGGHRGGEIIA"
BASE COUNT          317 a          596 c          642 g          363 t
ORIGIN      
        1 ggcgatgatc tcgccgccgc ggtggccgcc ctcggggccg aggtcgacga tccagtcggc
       61 cgtcttgatc acgtcgagat tgtgttcgat taccaccacg gtattgccgt cgtcgcgcag
      121 gcgatgcagc acgcgcaaca actgctcgat gtcgtggaaa tgcaatcccg tggtcggctc
      181 gtcgaggatg tacagcgtgc gtccggtatc gcgcttggac agctcgcgcg acaatttcac
      241 gcgctgcgct tcgccgccgg acagcgtcgt cgcgctctgg ccgagcttga tgtagctcaa
      301 gcccacgtcc atcaaggttt cgagttttcg cgccagcgcc ggaatgttct cgaacaattt
      361 cagcgcgtcc tcgacggtca tttccagcac gtcggaaatc gtgtggccct tgtacaggat
      421 ctccagcgtc tcgcggttgt agcgtttgcc gtggcagacg tcgcagggca cgtaaacgtc
      481 gggcaggaag tgcatctcga ccttgatcag gccgtcgcct tcgcaggctt cgcagcggcc
      541 gcccttgacg ttgaagctga aacgaccggg ttggtagccg cgcgcgcgcg cttccggaac
      601 ctgcgcgaac aattcgcgca gtggcgtgaa caagccggtg taggtcgcgg gattggagcg
      661 cggcgtgcgt ccgatcggcg actggtcgat gtccacgacc ttgtcgaaat gctccatccc
      721 ttcgatcttg cgaaacggcg cgggcttttc gttggcgccg ttgagttcga cggctgcatg
      781 tcggaacaag gtgtcgttga tcagtgtgga tttgcccgag cccgacacac cggtcacgca
      841 cacgaacagg ccggcgggaa tctccaggtc cacgtccttg agattgttgc cggacgcgcc
      901 ggacacgcgc agcatcagct gcggatccgg cgcgcggcgc ttgcgcggca ccggaatgga
      961 gcgccgtccg gacaggaaat cgccggtctg cgagcgcttc gccgagagca cgtccttgag
     1021 cttgccctgg gcgacaatct cgcccccgtg cacgccggcg ccggggccga tgtcgacaac
     1081 gtgatcggcg gcgcggatcg cgtcctcgtc gtgctcgacc acgatcaccg tgttgccaag
     1141 gtcgcgcagg cgcgtgagcg tgccgagcag gcgctcattg tcgcgctggt gcaggccgat
     1201 ggacggttcg tcgagcacgt acatgacgcc gaccaggccc gcgccgatct gtgaggcgag
     1261 gcggatgcgc tgcgcttcgc cgccagacag cgaatcggcc tgacgatcca gagtcaggta
     1321 atccaggccc acgtcgacaa ggaagcgcag gcgctcgcgg atttccttga cgattttcgt
     1381 cgcgatttcg ccgcgccagc ctggcagggt gagcgcgtgg aaaaattcca atgcctggtc
     1441 gatcggcagt ccggtgagca ccggcaggtt gctctcgccg acgaagacgt tgcgcgcact
     1501 gcggttcagg cgttcgccat gacagtcggg gcagggacgt tcgctgatga attttgacag
     1561 ctcctcgcgc acggccggcg attcggtttc gcggtagcgg cgttcgagat tcggcacgat
     1621 gccctcgaag cggtgcttgc gcgtgacgtt gccgccggat tccgtgagat acctgaacgc
     1681 gatctgctcg tcgccggagc cgtacaacac ggcgtggcgg attccctccg gcagttgttc
     1741 ccatggcgtg tcgatgtcga acttgtagtg ccgtgccagc gacaggatca tctggaagta
     1801 gtacatgttg cggcgatccc agccgcgcac cgcgccggcg gcgagactga gattcggatg
     1861 cacgacgacg cgcgccgcgt cgaaaacctg ggtgacgccg aggccgtcgc aggtgccg
//
LOCUS       DASRVO010000229         1931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_266704, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000229 DASRVO010000000
VERSION     DASRVO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266704"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFF05_02785"
     CDS             <1..792
                     /locus_tag="VFF05_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50760.1"
                     /translation="RAYEATQRFEQARDALKHAYDLAKGDPDVAVAYAEALALSSPTR
                     RIEGEPRQILDAALKSAPDNQRGLWLLGISENQAKRYDSAIAAWKHLLGVLPKDSDIA
                     PSVQQQIARAEAERDGKPLPAEEPQEANAAATAATTAPAPASASESGAQLHVEVALDP
                     KLAGKLAAGDVLFVYAKAASGPPMPLAIQRMDASKLPVTVTLTDGMGMLPSMKLSQFP
                     QVVIGARVSKSGNAMPQSGDLQVLSKPMAVTTTTPIKLTIDQVVP"
     gene            802..1920
                     /locus_tag="VFF05_02790"
     CDS             802..1920
                     /locus_tag="VFF05_02790"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZW50761.1"
                     /translation="MPDARRYFELSSPFAMKRGGTLVGARLAYETWGTLNAARDNAIV
                     ILTGLSPSAHAAANAIDPTPGWWEEMLGPGKPIDTNRWFAICVNSLGSCKGSTGPASV
                     DPVTGNVYKLTFPELTLEDAGNSAFELVRGLGIEHLACLIGNSMGGMSALGWLVNHPG
                     SVRAHVSISTAPQAQPFAIAIRSLQREAIRLDHNWNDGNYDDANYPEAGMSIARKLGV
                     ITYRSAMEWVGRFARIRLDAEARPDDDPFGVEFQVESYLAGHARRFVRSFDPNCYLYL
                     SRASDWFDVSEYSGAEPGHGSVSSALKRIDVERALVIGVSTDILFPLSQQEEIADGLR
                     AAGAHVDFVALDSPQGHDAFLVDIPRFGAAIAAFLATL"
BASE COUNT          366 a          652 c          591 g          322 t
ORIGIN      
        1 cgcgcctacg aggcgacgca gcgtttcgag caggcgcgcg atgcgctcaa gcacgcctac
       61 gacctcgcca agggcgatcc ggatgtcgcc gtcgcctatg ccgaagcgct ggcgctgtcg
      121 agtccgacgc ggcgcatcga aggcgaacca cggcagatcc tggatgccgc attgaaatcc
      181 gcgccggaca accagcgcgg cctgtggctg ctcggcatca gcgaaaatca ggccaagcgc
      241 tacgattcgg ccatcgccgc gtggaaacat ttgctcggcg tactgcccaa ggattcggat
      301 atcgccccat cggtgcagca acagatcgcg cgcgccgaag ccgaacgcga cggcaagccg
      361 ctgccggcgg aggaaccaca ggaagcgaat gctgccgcaa ccgcagcgac tacggctccc
      421 gcgccagcat ccgcttccga aagcggtgcg caactgcatg ttgaagtcgc gctggatcca
      481 aaactcgccg gcaaacttgc cgccggcgac gtgctgttcg tctacgccaa ggccgccagt
      541 ggcccgccga tgccgctcgc gatccagcgc atggacgcga gcaaattgcc ggtcacagtc
      601 acgttgaccg acggcatggg catgctgccg agcatgaaat tatcgcaatt tccacaagtg
      661 gtcatcggcg cacgcgtatc caaatccggc aacgcgatgc cgcaaagcgg tgacttgcag
      721 gtgctgtcga aacccatggc ggtgacgacc acgacgccga tcaagctgac gatcgatcaa
      781 gtcgtgccgt aaaatcggcg gatgcccgac gcccgccgat atttcgagct ctcctctccc
      841 tttgcgatga aacgcggcgg caccctcgtc ggcgcgcgcc tcgcctacga aacctggggc
      901 acgctgaacg cggcacgcga caacgcaatc gtgatcctca ccggcctgtc gccgagcgcg
      961 cacgcggcgg cgaatgccat cgatccgacg cccggctggt gggaagaaat gctcggcccc
     1021 ggcaagccga tcgacacgaa tcgctggttc gcgatctgcg tcaattcgct cggcagttgc
     1081 aaaggctcca ccggaccggc ctcggtcgat ccggtcacgg gaaacgtata caaattgact
     1141 tttccggaat tgacgctgga agacgccggt aattccgcat tcgagctggt acgcggactc
     1201 ggtatcgagc atctggcctg cctgatcggc aattccatgg gcggcatgag tgcgctcggc
     1261 tggctcgtca accacccggg cagcgtgcgc gcgcacgtca gcatctccac cgcaccgcag
     1321 gcgcagccgt tcgccatcgc gatccgctcg ctgcaacgcg aggcgatccg gctcgaccac
     1381 aactggaacg acggcaacta cgacgacgcg aactatcccg aagccggcat gtcgatcgcg
     1441 cgcaagctcg gcgtgatcac ctatcgctcg gccatggaat gggtcggccg cttcgcgcgc
     1501 atccgcctcg acgccgaggc gcggcccgac gacgatcctt tcggcgtcga attccaggtc
     1561 gaatcgtacc tggccggaca cgcgcgccgc ttcgtgcgca gcttcgatcc aaactgttat
     1621 ctgtacctgt cgcgcgccag cgactggttc gatgtatccg agtattccgg cgcggagccg
     1681 ggacacggca gtgtctccag cgcattaaaa cgcatcgatg tcgagcgcgc gctggtgatc
     1741 ggcgtgtcca ccgacatcct gttcccgctg tcgcagcagg aagaaatcgc cgacggcctg
     1801 cgtgccgccg gcgcgcacgt tgacttcgtg gcgctggatt cgccgcaggg ccacgacgcg
     1861 tttctggtcg atattccgcg ttttggcgcc gccatcgcgg cgtttctggc cacgttgtag
     1921 aatccgtcca t
//
LOCUS       DASRVO010000230         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_7007, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000230 DASRVO010000000
VERSION     DASRVO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7007"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFF05_02795"
     CDS             complement(<1..834)
                     /locus_tag="VFF05_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZW50762.1"
                     /translation="MVLAAFGAHAAKPDLDYERLRASLDSLAADPVLGKLASGERGLA
                     EQAVQLLVTDRSGGKDARAFRVYMAERRVDIAYAAAQAANQQNQLDELGREHDRILLQ
                     AAQREAEQARADAERQRLQNMIRAEEVDRLRAAGEQSAQEAQAAQAQADQAQKLIAAQ
                     AKATELARKEAQLAEAANTDLRRRLNNLTATRGAEGMQMTLDDIAFAPGQARLRPAAK
                     SSLGKLVAFVNRDPAKPIRIEGHTDSSGDTHANQVLSQRRADAVRDALIAAGVAANRI
                     TT"
     gene            complement(861..1259)
                     /locus_tag="VFF05_02800"
     CDS             complement(861..1259)
                     /locus_tag="VFF05_02800"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HZW50763.1"
                     /translation="MALMTPNWRKIHCAVLTGALALAGCGPTRPPDTGLDDAAQVLAT
                     ARAAGAPTYAPLELRSAEDHLSQARAFAEKRHYEDSARLAKESQADSELAQVKARLGK
                     LREQVDAAARENARLQQAAGAGSPTTGGVQ"
     gene            1389..2492
                     /locus_tag="VFF05_02805"
     CDS             1389..2492
                     /locus_tag="VFF05_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZW50764.1"
                     /translation="MDIGYFLKLMTEKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPG
                     GMVKKIAYSLMDEGQVPQFERDLELNLAIAVKDAGRFRINVFKQRGEVGMVIRAIKSE
                     IPSIEQLRLPVLFKELVMEPRGLVLLVGATGSGKSTTLASMIDHRNTNVSGHILTIED
                     PIEYLHRHKKSVVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDVETMEAAIAFS
                     ETGHLCLATLHSNNADQTLERILNFFPESAHRNILMNLALNLRAVISQRLVIGKDGRR
                     LPAAEVLLNTPLIRDMIRRGQVHEIKEAMDRSLQEGMQTFDQALMTMYKDGKIDLEEA
                     LSKADSRDGLALKIRLSEGGDAAMPEAYDAAAF"
     gene            complement(2489..3157)
                     /gene="maiA"
                     /locus_tag="VFF05_02810"
     CDS             complement(2489..3157)
                     /gene="maiA"
                     /locus_tag="VFF05_02810"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HZW50765.1"
                     /translation="MAAERLKLYGYWRSSAAYRVRIALNLKGLPYEIAPVHLTENGGA
                     QHADAFRKLNPQELIPVLVDGERIVRQSLAIIEYLEEAYEGNAKLLPTTVRERARVRG
                     LAQIVACDIHPLGNLRVLQYLEREFNTPQVERERWAQHWIHEGFVAFERLLAENPSTG
                     LFCEGDEPTMADICLVPQVYNARRWSVDMAPFPTIVRIDTECAKLPAFASARPENQPD
                     APKA"
     gene            complement(3229..4239)
                     /locus_tag="VFF05_02815"
     CDS             complement(3229..4239)
                     /locus_tag="VFF05_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HZW50766.1"
                     /translation="MKLASLKEGGRDGTLVVVDRDLSRAVRVTDIARTLQAALEDWDN
                     TAPRLNRVYELLHDAGKAQTINGEDVFALDVAALAAPLPRAYEFVDGSAYLPHVERVR
                     RARGAQVPESFYVDPLMYQATSAGFLGPRDPVLATSEDYGIDLEAEVVVVTDDVPMAV
                     TPAQAAGHIQLIGLVNDVSLRNLIPAELAKGFGFLQSKPRSALSPVFVTPDELGDAWR
                     DCKVHLPMRTWINAKWFGEADCGVDMQFSFADLVAHVAKTRSLSAGTLVGSGTIANQD
                     TGKGASCLAEQRTVETLRDGKPSTPFLKFGDTLRIEITDATGRSIFGAIEQTVVQAKN
                     PG"
     gene            complement(4250..4654)
                     /locus_tag="VFF05_02820"
     CDS             complement(4250..4654)
                     /locus_tag="VFF05_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HZW50767.1"
                     /translation="MSVTLLDANVLIALYDDDHTHRVTVADWFVGLTGEFATCPIVEG
                     ALVRWIVRVIGREGVDIARHELGKLHRDPRHRFWSDDLSHRDMAWDGVLGHKQVTDAY
                     LAALARKHKGRLATLDRGLAALHQDVAELIPV"
BASE COUNT          830 a         1547 c         1511 g          861 t
ORIGIN      
        1 cgtggtgatg cggttcgcgg ccaccccggc ggcgatcagc gcgtcgcgca ccgcgtccgc
       61 gcggcgctgc gacagcacct ggttggcgtg cgtatcgccg ctgctgtcgg tgtggccctc
      121 gatgcggatc ggcttggccg gatcgcggtt cacgaacgcg acgagcttgc ccaaactcga
      181 tttcgccgcc gggcgcaatc tggcctggcc cggagcgaaa gcgatgtcgt ccaaggtcat
      241 ttgcatgcct tcggcgccgc gcgtggcggt tagattgttc aggcgccggc gcaagtccgt
      301 gttggcggcc tcggcaagct gcgcttcctt gcgcgccagt tccgtcgcct tggcctgggc
      361 agcgatcagt ttttgcgcct ggtcggcctg tgcctgcgcg gcctgggctt cttgcgcgct
      421 ctgctcgcct gcggcgcgca ggcgatcgac ttcctcggcg cggatcatgt tctgcaagcg
      481 ctggcgttcg gcatcggcgc gcgcctgctc ggcttcgcgt tgtgccgcct gcaacaggat
      541 gcgatcgtgc tcgcgaccga gttcgtcgag ctggttctgc tggttggctg cctgcgccgc
      601 ggcgtaggcg atatcgacgc gccgctcggc catgtagacg cggaacgcgc gtgcgtcctt
      661 gccgccggag cggtccgtca ccagcagttg caccgcctgt tcagcgagac cgcgttcgcc
      721 ggacgcgagc ttgcccagca ccgggtcggc ggcgagcgaa tccaggctcg cgcgcaagcg
      781 ttcgtaatcc agatcgggtt tggcggcgtg cgcgccgaag gcagccaaaa ccaaaacgat
      841 gccagcacag aggcgaacgt tcactggacg cctcccgtcg tagggctgcc ggcgccagcg
      901 gcctgctgca ggcgcgcatt ctcgcgcgcg gccgcatcga cctgttcgcg cagcttgcca
      961 agcctggcct tgacctgggc cagctcactg tcggcttggg attccttggc caggcgcgcg
     1021 gaatcctcgt agtggcgttt ctcggcgaag gcgcgggcct ggctcaggtg atcctcggcg
     1081 ctgcgcaatt ccagcggtgc atacgtcggc gctccggccg cccgcgccgt tgccaacacc
     1141 tgcgcggcgt cgtccaggcc tgtgtccggc ggccgcgtag gtccgcaccc cgccagggcg
     1201 agtgcgccag tgagaacggc gcaatggatt tttcgccaat ttggtgtcat aagagccata
     1261 atcttgaacg ggggcaggcg cgggtgagcc tagtccgaga tcggaagcgg cgcaagccgc
     1321 gtgccgtggc ctctcacccg ccccggcaaa cgcgccgcgg cgcggcgcat gtgcaatgcg
     1381 aggcaatcat ggatatcggc tattttctga aactgatgac cgaaaaaggt gcgtcggaca
     1441 tgttcctgac caccggcgcg ccggtcaaca tcaaggtcga gggcaagctc tatccgcttg
     1501 gcaacaccgg cctgcccggc ggcatggtga agaaaatcgc ctactcgctg atggacgaag
     1561 gccaggtgcc gcagttcgag cgcgatctgg aactcaacct cgccatcgcc gtcaaggacg
     1621 ccggccgctt ccgcatcaac gtgttcaagc agcgcggcga agtcggcatg gtgattcgcg
     1681 cgatcaagag cgaaattcct tcgatcgagc agctgcgcct gccggtgctg ttcaaggaac
     1741 tcgtgatgga gccgcgcggc ctggtgctcc tggtcggcgc caccggttcc ggcaaatcga
     1801 ccacgctggc gtcgatgatc gaccatcgca acaccaacgt gtccggccac atcctcacga
     1861 tcgaagaccc gatcgaatac ctgcaccggc acaagaaatc cgtggtcaac cagcgcgaag
     1921 tcggcctcga cacgcacagc taccacgagg cgctgaagaa cgcgatgcgc gaggcgccag
     1981 acgtcatcat gatcggcgaa atccgcgatg tcgaaaccat ggaagcggca atcgcgtttt
     2041 ccgaaaccgg gcatctgtgc ctggccacgc tacactccaa caacgccgac cagacgctcg
     2101 agcgcatcct caacttcttc cccgaatccg cgcaccgcaa catcttgatg aaccttgcgc
     2161 tgaacctgcg cgccgtgatt tcgcagcgcc tggtcatcgg caaggacggt cgccgcctgc
     2221 ctgcggccga agtgctgctg aacactccgc tgatccgcga catgatccgc cgcggccagg
     2281 ttcacgagat caaggaagcg atggaccgca gcctgcagga aggcatgcag accttcgatc
     2341 aggcgctgat gacgatgtac aaggacggca agatcgacct cgaggaggcc ctgtccaagg
     2401 ccgattcgcg cgacggactg gcactgaaga ttcgcctgtc cgaaggcggc gacgcggcga
     2461 tgccggaggc gtacgacgcc gcggcgttct aggctttcgg cgcgtccggc tggttctccg
     2521 gccgcgcgct cgcgaacgcg ggcagcttgg cgcattcggt gtctatgcgc acgatggtcg
     2581 ggaacggcgc catgtccacc gaccagcgcc gcgcgttgta gacctgcggc accaggcaga
     2641 tgtcagccat ggtcggctcg tcaccttcgc agaacaaccc ggtcgaggga ttttctgcga
     2701 gcaggcgctc gaacgcgacg aagccttcgt ggatccagtg ctgtgcccag cgttcgcgtt
     2761 ccacttgcgg cgtgttgaac tcgcgttcca ggtattgcag cacgcgcaga ttgccgagcg
     2821 ggtgaatgtc gcaggcaacg atctgggcaa gtccgcgaac acgcgcgcgt tcgcgcaccg
     2881 tggttggcag aagcttcgcg ttgccctcgt aggcttcttc cagatattcg atgatcgcca
     2941 gggactggcg gacgatgcgc tcaccgtcga ccaaaaccgg gatcaattcc tgcggattga
     3001 gtttgcgaaa cgcatccgca tgttgcgcgc cgccattttc ggtcaaatgc acgggcgcaa
     3061 tctcgtaggg aaggcccttg agattgagcg cgatgcgcac gcggtaagcc gcgctggatc
     3121 gccagtaacc gtacagtttc agacgttcgg cagccattcg acacccccgt gcaacggcgc
     3181 aatatgcgca gcctgccgca gttgcgggca aaaatcaaag aatgttcgtc atccgggatt
     3241 cttcgcctgc acgaccgtct gttcgatcgc cccgaaaatg ctgcgcccgg tggcatccgt
     3301 gatctcgatg cgcaaggtgt cgccgaattt caggaatggc gtggacggtt tgccgtcgcg
     3361 cagcgtttcc acggtgcgct gctcggccag gcacgatgcg cccttgcccg tatcctgatt
     3421 ggctatggtg cccgatccga cgagggtgcc ggcggacaac gaccgggtct tggccacatg
     3481 cgcgaccagg tcggcgaaac tgaactgcat gtccacgccg cagtcggctt cgccgaacca
     3541 ttttgcgttg atccaggtac gcatcggcaa atggactttg cagtcgcgcc acgcatcgcc
     3601 gagttcgtcc ggcgtgacga acaccggcga cagcgccgag cgcggtttgg actgcagaaa
     3661 gccgaaaccc ttggcgagtt ccgccggaat caggttgcgc agggaaacat cgttgaccag
     3721 tccgatcaac tggatgtgtc ccgccgcctg cgccggcgtt accgccatcg gcacgtcgtc
     3781 ggtgacgacc acgacttcgg cttcgaggtc gatgccgtaa tcttcgctgg tcgccagcac
     3841 cggatcgcgt gggccgagaa aacccgcgct ggtcgcctga tacatcagcg ggtcgacgta
     3901 gaaactttcc ggcacctgcg cgccgcgtgc gcggcgcacg cgctcgacgt gcggcagata
     3961 cgccgagcca tccacgaact cgtaagcgcg cggcagcggc gcggcaagtg cggcgacatc
     4021 aagcgcgaac acatcctcgc cgttgatcgt ctgtgccttg cccgcgtcgt ggagcagctc
     4081 gtacacgcga ttcaggcgcg gcgccgtgtt gtcccaatcc tcgagcgcgg cttgcagcgt
     4141 gcgcgcaatg tccgtcacgc gtaccgcgcg gctcaaatcg cggtcgacga cgacgagcgt
     4201 gccgtcgcgt ccaccttctt tcaggcttgc cagtttcatc gcgaattcct caaaccggga
     4261 tcaattcggc cacatcttgg tgcagggcgg ccagtccgcg gtcaagcgtc gccaggcgcc
     4321 ccttgtgctt gcgtgccagc gcggcgaggt aggcgtcggt gacctgcttg tggccgagca
     4381 cgccgtccca tgccatgtct cgatgggaca aatcgtcgga ccaaaaccga tggcgagggt
     4441 cgcggtgaag tttccccaat tcgtgcctgg caatatcgac gccttcgcga ccaatgactc
     4501 gcacgatcca gcgtacgagc gcaccttcaa cgatcgggca cgttgcgaac tcaccagtca
     4561 gaccaacaaa ccagtcggcg acggtgacgc gatgcgtgtg atcgtcgtca tacagcgcga
     4621 tcaacacgtt agcgtcgagc aatgtaacgc tcatcgtcct cttcttcgag cgccctcacg
     4681 tcgtcgatcg tgaccggctt cccggaacgg atgacaccca atccggtgcg tgggtcaata
     4741 cggtacttc
//
LOCUS       DASRVO010000231         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_318886, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000231 DASRVO010000000
VERSION     DASRVO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318886"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFF05_02825"
     CDS             complement(<1..844)
                     /locus_tag="VFF05_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZW50768.1"
                     /translation="MTLTLVLVVLAVALIFEYINGFHDTANSIATVVATKVLSPGQAV
                     LLAACTNLLGAMWGTAVAKTIASGLIDTGVVAMTPEILICALLGATIWDLITWWLGLP
                     SSSSHALVGGLCGAALAAADGNWQAIVWSVPADHWWHGKGVLNKVVLPMIASPLAGFV
                     IGFLVMGLLFALLSFFNGLGGAATRATRPRTINAVFGKLQILSSGFMGLSHGMNDAQK
                     TMGIIALALAGATAAGSLDHLPAWLHFLRVPTDAQGQFGIAMWIKVVCALTMAAGTMI
                     GGWRI"
     gene            complement(854..1483)
                     /locus_tag="VFF05_02830"
     CDS             complement(854..1483)
                     /locus_tag="VFF05_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pit accessory protein"
                     /protein_id="HZW50769.1"
                     /translation="MFSLQTIFGKGDKFYGLLEASADAARQSTRALITMLNGKSPNPS
                     LDEFKVARRREKEIMAQISHALVNTFVTAIEREDIEALSGALYKIPKTVEKFAERYAL
                     FAEQLVDVDYASRAAMLEKGVEIIVEMVRQLRRGMKLEEMKALNDRMQAIESEADRLI
                     LELYKEMFSETSNAVRLLLRKDLFELLEKAIDRCRDAGNVIYAIVLKNS"
     gene            1622..>2657
                     /locus_tag="VFF05_02835"
     CDS             1622..>2657
                     /locus_tag="VFF05_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZW50770.1"
                     /translation="MILEIALIVLLALLNGFFALSEMALMTSRRSRLKHLARSSRRAQ
                     IALKLAQTPERFLATVQVFITLIGIGTGTALGARLGAEFAHLFDATPLEWLAPYAPAL
                     GVTLSVSAITFANMLLGELVPKRVALVNPERYAVATAVPMRVLTWAATPFAFGLIWLT
                     RLILRALRMDRVGAEQVSEEEIRLLVSESADQGVIDSDERNMVNRVLRLGDRAVDSLM
                     TPRPRIAWLDAAASLEENLAVMRDTPFSRYPVYRGNDKDVLGVLSLKRLPAMLGNAKI
                     DLFAKLAQPLYVPGTARALDLLEEFRDAETRLALVVDEYGDVEGLVTLNDVLGAVVGQ
                     GAAPVPATQTQ"
BASE COUNT          462 a          915 c          827 g          453 t
ORIGIN      
        1 tgatgcgcca gccgccgatc atggtgcctg cggccatggt cagggcgcag acgaccttga
       61 tccacatggc gatgccgaac tgaccttgcg catcggtcgg cacgcgcagg aaatgcagcc
      121 acgccggcag atgatccaga gaaccggccg cggtcgcacc ggccagcgcc agcgcgatga
      181 tgcccatggt tttctgcgcg tcgttcatgc cgtgcgacag gcccatgaaa cccgacgaca
      241 gtatctgcag cttcccgaac acggcattga tcgtgcgcgg ccgggttgcg cgcgtggccg
      301 cgccgccgag tccgttgaag aacgacagca gcgcgaacaa cagtcccatc accaggaatc
      361 cgatcacaaa cccggccagc ggcgacgcga tcatcggcag gacgaccttg ttcaacacgc
      421 ccttgccatg ccaccaatga tcggccggaa ccgaccagac gatggcctgc cagttgccgt
      481 ccgccgccgc caacgccgcg ccgcacagcc cgccaaccag cgcgtgcgag gaactcgacg
      541 gcaggcccag ccaccaggtg atcagatccc agatcgtcgc gccgagcagc gcgcagatca
      601 ggatttccgg cgtcatcgcc accacgccgg tgtcgatcag gccggaggcg atggtcttgg
      661 ccacggccgt gccccacatc gcgccgagca ggttcgtgca ggccgcaagc agcaccgcct
      721 ggccgggact gagcaccttg gtcgcgacca ccgtggcgat ggaattggcg gtgtcgtgga
      781 agccgttgat gtactcgaag atcagggcga ccgcgaggac gacgaggacc agggtgaggg
      841 tcacggcgag ccctcaggaa ttcttcagca cgatcgcgta aatcacgttg ccggcgtcgc
      901 ggcagcggtc gatggccttt tccagcaatt cgaacagatc cttgcgcagc agcaggcgca
      961 ccgcattgct ggtttcagag aacatctcct tgtacaactc caggatcagg cggtcggcct
     1021 cgctctcgat cgcctgcatg cggtcgttga gcgccttcat ttcctcgagc ttcatgccgc
     1081 ggcgcaactg gcgcaccatc tcgacgatga tctccacgcc tttctcgagc atcgccgcgc
     1141 gcgaggcgta gtccacatcc accaattgtt cggcaaacaa ggcgtagcgt tcggcgaatt
     1201 tctccaccgt cttcggaatc ttgtacagcg cgccggacag cgcctcgatg tcctcgcgct
     1261 cgatcgcggt gacgaaagta ttgaccaggg catggctgat ctgcgccatg atttcctttt
     1321 cccggcgccg cgcgactttg aattcgtcca gcgaaggatt cggcgacttt ccgttcagca
     1381 tcgtgatcaa cgcgcgcgtg ctctgccgcg cggcgtccgc gctggcctca agcaggccgt
     1441 agaacttgtc gcctttgccg aaaatcgttt gcagggagaa catgcgctcg cctcgtccgg
     1501 attgaatgcg cgggccaggt tggcggcgcg ggcgcagttt accgttttgc ggcggccttt
     1561 gccgggccgg cgcggcggcg tgctctgcta gactccgacc catgactaca cggtccgccg
     1621 gatgatcctg gaaatcgcgc tcatcgtcct gctcgccctg ctcaacggct ttttcgcgct
     1681 cagcgaaatg gccttgatga cgtcgcggcg cagccgtctc aagcacctcg cgcgcagcag
     1741 ccggcgcgcg cagatcgcgc tgaaactcgc gcagacgccc gaacgcttcc tcgccacggt
     1801 gcaggtgttc atcaccctga tcggcatcgg caccggcacc gcgctgggcg cgcgcctcgg
     1861 cgcggaattc gcgcacctgt tcgacgcaac gcctctggaa tggctcgcgc cgtacgcacc
     1921 cgcgcttggc gtgacgctga gcgtatccgc catcacgttc gcgaacatgc tgctcggcga
     1981 gctcgtgccc aagcgcgtcg ccctggtcaa cccggaacgc tacgccgtgg cgacggcagt
     2041 gcccatgcgc gttctgacct gggccgccac gccgttcgca ttcgggctga tctggctcac
     2101 ccgcctgatc ctgcgcgcgc tgcgcatgga tcgcgtcggc gccgaacagg tcagcgaaga
     2161 ggaaatccgc ctgcttgtgt ccgagagcgc cgatcagggc gtgatcgact ccgacgagcg
     2221 caacatggtc aaccgcgtgc tgcgcctggg cgatcgcgcg gtggacagcc tgatgacgcc
     2281 gcgcccgcgc atcgcctggc tcgatgccgc cgcgtcgctc gaagaaaacc tcgcggtgat
     2341 gcgcgacacg ccgttttcgc gctatcccgt gtaccgcggc aacgacaagg acgtgctcgg
     2401 cgtgctcagc ctgaaacgcc tgccggccat gcttggcaac gccaaaatcg atttgttcgc
     2461 gaagctcgcc caaccgctct acgtgcccgg caccgcgcgc gcactggatc tgctcgagga
     2521 attccgcgac gcggaaaccc ggctcgcact cgtcgtggac gaatacggcg atgtcgaggg
     2581 gctggttacg ctcaacgacg tgctcggcgc cgtggtcgga caaggcgccg cgccggtgcc
     2641 ggccacgcaa acccagc
//
LOCUS       DASRVO010000232         1330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_240412, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000232 DASRVO010000000
VERSION     DASRVO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240412"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFF05_02840"
     CDS             complement(<1..224)
                     /locus_tag="VFF05_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HZW50771.1"
                     /translation="MDAFRHLIDAPQLLALAAALGWASGVRLYLAVFLVGGAGYMGWI
                     DLPQGLQVLQHPGVLAAAGLMAFTEFFADK"
     gene            complement(231..1016)
                     /locus_tag="VFF05_02845"
     CDS             complement(231..1016)
                     /locus_tag="VFF05_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HZW50772.1"
                     /translation="MKHLELRFDGGVATVTLNRPEVRNAFNDEVIAELTAVFSELDER
                     TEVRCIVLAGNGAAFCAGADLNWMKRMANYTRDENLADAAALAHMLRAIYRCPKPTIA
                     RIQGDVYAGGLGLVAACDIAVSVDGAHYCLSEVKLGLIPATISPYVIRAMGARAAHRY
                     FLTAERFSAAEALRIGFVHEVVGADQLDAKVAELAQALVQAGPAAVKACKALVQDVAG
                     KDIAQLLIDRTVENIADIRVSDEGREGIQSFLQKRKPGWISQG"
     gene            complement(1029..>1330)
                     /locus_tag="VFF05_02850"
     CDS             complement(1029..>1330)
                     /locus_tag="VFF05_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZW50773.1"
                     /translation="QTIRQRLSMLDAKTRAARQFMYHCAWRVTQGHDIVQDVSMLKAL
                     TGELVNEVVQTCQQFHGGMGYIRETAVERLWRDARVLAIGGGATEVMLEEVAKRY"
BASE COUNT          214 a          476 c          407 g          233 t
ORIGIN      
        1 atcttgtccg cgaagaattc ggtgaaagcc atgagccccg cggcggccag cacgcccgga
       61 tgctgcagca cctgcaggcc ctgcggcagg tcgatccatc ccatgtaacc cgcaccgccc
      121 accaggaaca cggcgagata gaggcgcacg ccgctcgccc agcccagcgc cgccgccagc
      181 gccagcaatt gcggcgcgtc gatcaggtgg cggaaagcat ccatcctgtt tcaaccttgc
      241 gatatccagc cgggcttgcg tttttgcagg aacgactgga tgccttcacg gccctcgtcg
      301 ctgacgcgga tgtcggcgat gttttccacc gtgcgatcga tcagcaactg cgcgatgtcc
      361 tttccggcga cgtcctgcac cagcgccttg cacgccttga cggccgcggg gccggcctgc
      421 accagcgcct gcgcgagctc ggccactttc gcatccaatt ggtccgcgcc cacgacctcg
      481 tgcacgaagc cgatgcgcaa tgcctcggcc gcgctgaacc tttcggccgt gaggaaatag
      541 cgatgcgccg cgcgcgcacc catggcgcgg atgacgtacg gactgatcgt ggcgggaatc
      601 aggcccagtt tcacctcgct caggcaatag tgcgcgccgt ccaccgacac ggcgatgtcg
      661 cacgccgcca ccagccccaa gccgcccgcg tacacgtcgc cctggatgcg tgcgatcgtc
      721 ggcttcgggc agcggtagat cgcgcgcagc atgtgggcca gcgccgccgc gtcggccagg
      781 ttctcgtcgc gcgtgtagtt cgccatgcgc ttcatccagt tgaggtcggc gccggcgcag
      841 aaggccgcgc cgttgcccgc gagcacgatg cagcggacct cggtgcgctc gtccaattcc
      901 gaaaacaccg ccgtcagttc ggcgatgacc tcgtcgttga acgcgttgcg cacttccggc
      961 cggttcagcg tcacggtcgc gacgccgccg tcgaagcgca gttccaggtg tttcatcgct
     1021 gccctctttc aataccgctt ggccacttct tccagcatca cttcggtggc gccgccgccg
     1081 atcgccagca cgcgcgcatc gcgccacagc cgctcgaccg cggtctcgcg gatgtagccc
     1141 atgccgccgt ggaactgctg gcacgtctgg accacttcat tgacgagttc gccggtgagc
     1201 gccttgagca tcgacacatc ctgcacgatg tcgtgcccct gcgtgacgcg ccatgcacag
     1261 tgatacatga attgccgcgc ggcacgggtc ttcgcgtcca gcatagagag gcgctggcgg
     1321 atcgtctgct
//
LOCUS       DASRVO010000233         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_162633, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000233 DASRVO010000000
VERSION     DASRVO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162633"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFF05_02855"
     CDS             complement(<1..252)
                     /locus_tag="VFF05_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HZW50774.1"
                     /translation="MTSQAPTARYTSGEELANSLTHGVGFLLAIVGLCGLMFAAGDVR
                     ETVTCLVYGVTLILVYATSTLYHGVHRPDAKQLLRTLDHV"
     gene            complement(341..787)
                     /locus_tag="VFF05_02860"
     CDS             complement(341..787)
                     /locus_tag="VFF05_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50775.1"
                     /translation="MRSIPVFAASVLLAGSALYSTCASAAGQIDCEMKFNLTGWAAIY
                     KHAEGHGTITCNNGRSFNVDIVAVGGGLTAGKYKVEGGTGKFSQVYDTSELFGSYAQG
                     EANAGVVKSGIAQALTKGNVSLALAGTGNGVNIGVSFGKFTISRAH"
     gene            923..1747
                     /locus_tag="VFF05_02865"
     CDS             923..1747
                     /locus_tag="VFF05_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZW50776.1"
                     /translation="MSAFDILVHLREPRGEPLAARAAFALAKRLDAHLHGLYVAPLGA
                     VAFSTPETVVFQVHEADRLYSEAQMQGAWWCDLLAAHAASGEWLVAQGETVEALCHCA
                     RWCDFVVAERPTLNPDAPTGWGTVSRTVFGAGVPVLVVPESAKLSTLGERILVAWNHS
                     REAMLAIRGALPLLARAAQVVVLDGAAQDDELGARHLPRLDLAAWLQRHGVSAQIRQF
                     APKNDYGANILAEARSMQADMIVMGAWGHSRIAQLVLGGTTRYLFQNSDLPLLVAH"
     gene            complement(1744..1980)
                     /locus_tag="VFF05_02870"
     CDS             complement(1744..1980)
                     /locus_tag="VFF05_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HZW50777.1"
                     /translation="MLELRSHCECCGKPLPADAPDAMICSFECTFCAECARERLRGVC
                     PNCGGNFVGRPIRPSALLQKYPPSTQRRPLVCID"
BASE COUNT          377 a          665 c          680 g          377 t
ORIGIN      
        1 cacatgatcg agtgtgcgca gaagttgttt ggcatccgga cggtgcacgc cgtggtacag
       61 cgtggacgtg gcgtagacga gaatcagggt gacgccgtag accaggcatg tgactgtttc
      121 gcgcacgtcg ccggctgcga acatcaaacc gcacaggccg acgatggcga gcaaaaagcc
      181 gacgccgtgg gtgaggctgt tggcgagttc ctcgccggag gtataacgcg cggtcggggc
      241 ttggctcgtc atgcgtccac cctaccgatt tttgtgcgct gcggcaataa aaacgcggcg
      301 cccttgcgag cgccgcgttt tcgatcagta cacgctcgaa tcagtgcgcg cgcgagatgg
      361 tgaacttgcc gaaagaaacg ccgatgttca cgccattgcc ggtgccggcc agggccaggg
      421 agacgttgcc cttggtcaaa gcttgagcga tgccggactt gaccacgccc gcattcgctt
      481 cgccttgcgc gtagctgccg aacaattcgc tggtgtcgta cacctgcgaa aacttgccgg
      541 tgccgccctc gaccttgtat ttgccggcgg tgaggccgcc gccgacggcg acgatatcga
      601 cgttgaagct gcgtccattg ttgcaggtga tggtgccgtg gccttcggcg tgcttgtaga
      661 tggcggccca accggtgaga ttgaacttca tttcgcaatc gatctggccg gcagcgctgg
      721 cgcacgtgga atacagcgcg gaccctgcga gcagaacgga tgcggcgaat acgggaatgc
      781 tgcgcatgtc gattctcctt cttgttgacc cttcggcagt ctcgcgcaag cggcctgaac
      841 ctacaacaac acgcggcgca aaggcggaac tttcgtgcac tgcgcgttaa gctaatgttc
      901 gagcctgcaa ggagtaaccg ccatgtccgc attcgacata ctcgtgcacc tgcgcgaacc
      961 acgcggtgaa ccgctcgccg cacgcgcggc tttcgcgctg gccaagcgcc tcgacgccca
     1021 tctgcacgga ctctatgtgg cgccgctcgg cgcggtcgcg ttcagcacgc cggaaaccgt
     1081 ggtgttccag gtccacgagg cggatcgttt gtattccgaa gcgcagatgc aaggcgcgtg
     1141 gtggtgcgac ctgctcgccg cgcacgccgc atccggcgaa tggctggtcg cgcagggcga
     1201 aacggtcgaa gcgctgtgcc attgcgcgcg ctggtgcgac ttcgtcgtcg ccgaacgacc
     1261 gacgctcaat cccgatgcgc caaccggctg gggcacggtg tcgcgcaccg tgttcggcgc
     1321 gggcgtaccc gtactcgtcg taccggaatc ggcgaaattg tcgacgctcg gcgagcgcat
     1381 cctcgtcgcc tggaaccaca gccgcgaagc gatgctggcg atccgcggcg cgctgccgct
     1441 gcttgcgcgc gcggcgcaag tcgtcgtact cgatggcgca gcccaggacg acgaactcgg
     1501 cgcgcgtcat ctgccgcgac tggacctggc cgcgtggctg caacgccatg gcgtgagcgc
     1561 gcaaatacgc caattcgcgc cgaaaaacga ctacggcgca aacatcctcg ccgaagccag
     1621 aagcatgcag gccgacatga tcgtcatggg tgcctggggt cattcgcgca tcgcccaact
     1681 cgtactcggc ggcaccacgc gctacctgtt ccagaacagc gatttgccgc tactggtcgc
     1741 acattagtcg atgcagacca gcggccgccg ctgggtcgac ggcggatatt tttgcagcag
     1801 cgcggacgga cggatcggcc gcccgacgaa gttgccgccg cagttcggac acacgccgcg
     1861 cagcctctcg cgcgcgcatt ccgcacagaa tgtgcattcg aacgaacaga tcatggcgtc
     1921 gggcgcatcg gcgggcagcg gcttgccgca acattcgcaa tgactgcgca attcaagcat
     1981 ggtcgcgatg ctggtgtttg ccgcgcgcgg gcggcaaatg cagcagtgca cgcagtttcg
     2041 tctcgacctg cgcgggctcg tcgccgccgc tgcgccgata gacgatgtcg cctttggca
//
LOCUS       DASRVO010000234         1094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_293484, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000234 DASRVO010000000
VERSION     DASRVO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293484"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..145
                     /locus_tag="VFF05_02875"
     tRNA            71..145
                     /locus_tag="VFF05_02875"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:103..105,aa:Gln,seq:ttg)
     gene            177..>1094
                     /locus_tag="VFF05_02880"
     CDS             177..>1094
                     /locus_tag="VFF05_02880"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZW50778.1"
                     /translation="MIHGPLKIFSGSAHPALAQEIAEHLDLPLGGARLHRFPDSEVSF
                     QIDENIRGTDVFIVQPTCSPVDEHIMEMCVMIDAFRRSSASRITAVIPYYGYARQDRK
                     DKPRVPISAKLVANLLSAAGANRVLTMDLHKAQIQGFFDIPVDHLFAAPVVIEYLARQ
                     NFGPVTIVSPDAGGAERARAYAKRIDAELAIIDKRRSEDGTAEVMNVVGDVKGRVCVI
                     ADDIIDTAGTIQKAAQALMDNGASRVLASAVHGVLSGPAISRINAAPIDKLIVTNTIP
                     LNGDKAACEKIVVLSVARLLAQAIRSIHEE"
BASE COUNT          197 a          310 c          392 g          195 t
ORIGIN      
        1 agcttcaaat tcgccgagag agtgccagac gcggtcgtgg tgcgacgccg gcgcgggggt
       61 gcgggggtgt tggggcgtgg ccaagcggta aggcgcggga ctttggatcc cgtattcgaa
      121 ggttcgaatc cttccgcccc aaccagcttt cgcctgggtg ccggccgatt gctgcgatga
      181 ttcacggtcc actgaagata ttctcgggca gcgcgcatcc cgcgctggca caagagattg
      241 cggagcatct cgatctgccc ctgggcggcg cgaggctgca tcgctttccc gacagcgaag
      301 tctcgtttca aatcgacgag aacattcgcg ggaccgacgt gttcatcgtg cagccgacct
      361 gtagcccggt ggacgagcac atcatggaga tgtgcgtgat gatcgacgcg tttcgccgct
      421 cgtcggcgtc gcggattacg gcggtcatcc cgtattacgg ttatgcccgg caggaccgga
      481 aagacaagcc gcgcgtgccg atctcggcca agctggtggc caacttgttg tcggcggccg
      541 gtgcgaaccg ggtgctgacg atggatctgc acaaggcgca gatccagggg ttttttgata
      601 ttccggtgga ccatctgttc gcggcgccgg tcgtgatcga gtatctggcg cggcagaatt
      661 tcgggccggt gaccatcgtg tcgccggacg cgggcggcgc cgaacgggcg cgcgcgtatg
      721 cgaagcggat cgatgcggaa ctggcgatca tcgacaagcg ccgcagcgaa gacggcacgg
      781 cggaagtgat gaacgtggtc ggcgacgtca aggggcgagt ctgcgtgatc gcggatgaca
      841 tcatcgacac ggccggcacg attcagaagg ccgcgcaggc gctgatggac aacggcgcga
      901 gccgggtgct cgcgagcgcg gtgcacggtg tgttgtcggg gccggcgatc tcgcggatca
      961 acgcggcgcc gatcgacaag ctgattgtga ccaacacgat tccgctgaac ggcgacaaag
     1021 cggcgtgcga aaagattgtg gtgctgtcgg tcgcgcgcct gctcgcgcag gcgattcgca
     1081 gcatccacga agag
//
LOCUS       DASRVO010000235         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_162776, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000235 DASRVO010000000
VERSION     DASRVO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162776"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2208
                     /locus_tag="VFF05_02885"
     CDS             <1..2208
                     /locus_tag="VFF05_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW50779.1"
                     /translation="VAEIDAQIGKPTVGFGPDCVDWMGVPLLRSGKVNGGLVVQSYTE
                     SARYTERDQELLTYVAQHIQTALERRFVHEELERRVAERTDALRDANRMLQQQVLERQ
                     RGERLQAALFRIAELANSSESLEEFYAAVHRAVGGLLYARNFYIALLSEDGQQLVFPY
                     SVDERDLKRVPRKLGTGLTEYVLNTRSVLLADSAGLERLYATGAAQPIGTRSRSWLGV
                     PLICGDRAVGVLAVQSHSEEHRYTPRDQELLTFVSYHIANALERKRSAESLKFANAEL
                     EQRVIERTSELAAANRELREHIAVRERMERQLKHETLHDALTGLPNRNFLLQRLTQAL
                     ADFARDSCHRFAVLFLDLDRFKVINDSVGHLIGDELLNEVGARIASCLEPRDLVARLG
                     GDEFAILLNDLENADDACALAQRVIDALNEPVRLGGKEIFSSTSIGIAMASSRYRKAE
                     ELLRDADVAMYRAKGEGRHRFAVFDEHLHKEAMQLMELESDLRRAIARSEFVPFFQPV
                     VRLSDRVTVGYEALLRWQHPERGLLAPGDFLAVAEETGAAEQIDWQMFERTFEVAGPL
                     LTGAAGFVAINVSGRHFRAETLDENLLALLRRHEIKPECVRIEVTERMLIDNPPAAKR
                     LLDAMRAHGIGVSLDDFGTGYSSLSYLHQYPVQALKIDRSFIAEMGVEDSGSVAIVRA
                     ILALAGALGMQAIAEGIETEAQHRILTQLGCQLGQGFLYARPQPAHVWLDRIG"
     gene            complement(2205..>2958)
                     /locus_tag="VFF05_02890"
     CDS             complement(2205..>2958)
                     /locus_tag="VFF05_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZW50780.1"
                     /translation="LGRTLAASALFTGNVKFDGALSIQIKNAGPVNLLFAECGSDGRV
                     RGLARWQGEIAEDFRLLDAQPAPLLAITIDNRASGQRYQGLVPVEAADFAALFEGYFE
                     RSEQLPTRVLLACTDGRCAGMMLQRLPGSAHADDDADAWNRVGHLLASASENELLTLA
                     PETLLYRLFHEENVRLHAARPLRFGCSCSRERVAAMLHSLGRAEAEAAVREDGVAEVT
                     CEFCNTQYRFDRVDLEQVFRSGQDVPAAPTQH"
BASE COUNT          523 a          964 c          955 g          516 t
ORIGIN      
        1 gttgcggaaa tcgacgccca gatcggcaag ccgacggtcg gctttggtcc cgactgcgtg
       61 gactggatgg gcgtgccgct gctgcgctcg ggcaaggtca acggtggcct ggtggtgcaa
      121 agctataccg aaagcgcgcg ctatacagag cgcgaccagg agttgctcac ctatgtcgcc
      181 cagcatatcc agacggcgct ggaacggcgc ttcgtgcacg aggaactcga acgtcgcgtg
      241 gccgagcgca ccgatgcgct gcgcgatgcc aaccgcatgc tgcagcagca ggtgctcgag
      301 cgccagcgcg gcgaacgcct gcaggcggcg ctgttccgca tcgccgaact cgccaacagc
      361 agcgagagtc tggaagaatt ctacgcggcc gtgcaccgcg ccgtcggcgg tcttctgtac
      421 gcgcgcaatt tctatattgc cctgctcagc gaggatggcc agcagctcgt gtttccgtac
      481 tccgtggacg agcgcgacct caaacgcgta ccgcgcaaac tcggcaccgg cctgaccgag
      541 tacgtgctca acacacgcag cgtactgctt gccgacagtg ccggactcga gcgcctgtac
      601 gccaccggcg cggcacagcc gatcggcacg cgttcgcgca gctggttggg ggtacctctg
      661 atttgcggcg atcgcgcggt cggcgtgctc gccgtgcaaa gccactccga agaacatcgc
      721 tacacgccgc gcgaccagga attgctgacc ttcgtttcgt atcacatcgc caacgcgctc
      781 gagcgtaaac gcagcgctga atcgctcaag ttcgccaacg cggaactcga gcagcgcgtg
      841 atcgagcgca ccagcgaact ggccgccgcc aaccgcgagc tgcgcgagca catcgccgtg
      901 cgcgaacgca tggagcgcca gctcaagcat gaaaccctgc atgatgcgtt gaccggattg
      961 ccgaaccgca atttcctgtt gcagcgcctg acccaggcgc tcgcggattt cgcgcgcgat
     1021 tcgtgccacc gcttcgccgt gctgtttctc gatctggatc gtttcaaggt catcaacgat
     1081 tcggtcggtc atctgatcgg cgacgagctg ctcaacgagg tcggtgcgcg catcgcgtcg
     1141 tgtctggaac cacgcgacct ggtcgcgcgc cttggcggcg atgaattcgc gatcctgctc
     1201 aacgatctcg aaaacgccga cgatgcctgc gcgctggcgc agcgcgtcat cgatgcgctc
     1261 aacgagccgg tccgactcgg cggcaaggaa attttcagtt ccaccagcat cggcatcgcc
     1321 atggcttcct cgcgctaccg caaggcggag gaactgctgc gtgacgccga tgtggccatg
     1381 taccgcgcca agggcgaagg ccgccaccgt ttcgccgtgt tcgacgagca cctgcacaag
     1441 gaagcgatgc agttgatgga gctggaaagc gatctgcgcc gcgccatcgc gcgcagcgag
     1501 ttcgtgccgt ttttccagcc ggtggtgcgt ttgtcggatc gcgtcacggt tggctacgag
     1561 gcgctgttgc gctggcagca tcccgagcgc ggcctgctcg caccgggcga tttcctcgcc
     1621 gtcgccgaag aaaccggcgc ggccgaacaa atcgactggc agatgttcga gcgcacgttc
     1681 gaggtcgcgg gaccgctgtt gaccggtgcg gccggtttcg tggcgatcaa cgtctccggc
     1741 cggcattttc gcgcggagac actcgacgaa aacctgctgg cgctgttgcg caggcacgag
     1801 atcaagcccg aatgcgtgcg catcgaggtc accgaacgca tgctcatcga caacccgccc
     1861 gcggccaaac gcctgctcga cgcgatgcgc gcgcacggca tcggcgtgtc gctggacgat
     1921 ttcggcaccg gctattcgtc gctgagctat ctgcaccagt atccggtgca ggcgctgaag
     1981 atcgaccgct cgttcatcgc ggaaatgggt gtggaagatt ccggcagcgt cgcgatcgtg
     2041 cgcgcgatcc tggcgctggc cggcgcgctc ggcatgcagg cgatcgccga aggcatcgaa
     2101 accgaggcgc aacaccgcat cctgacccaa ctcggctgcc agctcggcca agggttcctg
     2161 tacgcgcgtc cgcaacccgc gcacgtctgg ctggatcgta tcggctagtg ttgcgtgggt
     2221 gcagcgggca cgtcctgtcc gctgcggaaa acctgttcaa gatccacgcg gtcgaaacgg
     2281 tattgcgtgt tgcagaactc gcaggtcact tcggcgacgc cgtcctcgcg cacggccgcc
     2341 tcggcttcgg cacggcccag cgaatgcagc atcgccgcaa cgcgttcgcg cgaacaactg
     2401 caaccgaatc gcaacggcct ggccgcatgc aggcgcacat tttcctcgtg gaagaggcgg
     2461 tagagcaggg tttccggcgc gagggtcaac aactcgttct cgcttgcgct ggcgagcagg
     2521 tgcccgacgc gattccaggc atcggcgtcg tcgtcggcat gcgcgctgcc gggcaggcgt
     2581 tgcagcatca tgcccgcgca gcggccgtcg gtgcaggcta gtaacacgcg cgtgggcagt
     2641 tgttcggagc gttcgaaata accttcgaac agcgcagcaa aatctgcggc ttcgaccggc
     2701 acgagtccct ggtagcgttg tccgctggcg cggttgtcga tggtgatcgc cagcagcggt
     2761 gccggttgcg catcgagcag acggaaatcc tctgcgatct cgccctgcca gcgcgccagg
     2821 ccgcgcacgc ggccgtcgct gccgcattcg gcgaacagca ggtttaccgg cccggcattc
     2881 ttgatctgga tcgaaagcgc gccgtcgaat ttgacgttac cggtaaacag cgcgctcgcg
     2941 gcgagggtgc gtccaagc
//
LOCUS       DASRVO010000236         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_240643, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000236 DASRVO010000000
VERSION     DASRVO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240643"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFF05_02895"
     CDS             <1..598
                     /locus_tag="VFF05_02895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZW50781.1"
                     /translation="PNGKKIDADKAGVNVTDRGFIAADKQMRTNVPHIFAIGDIVGNP
                     MLAHKATHEGKVAAEVCAGLKSEFVARVIPSVAYTDPEIAWVGLTEGEAKKAGIAYGV
                     GKFPHAASGRAIGIARTEGFTKLIFDEATHRIIGAGVTCPNAGELISELALAIEMGCE
                     AGDIGLTVHPHPTLSESVGMAAEVYEGTITDLYIPKRK"
     gene            complement(599..946)
                     /locus_tag="VFF05_02900"
     CDS             complement(599..946)
                     /locus_tag="VFF05_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50782.1"
                     /translation="MIARIWRGITQADKADDYLAYLQETGLKDYANTSGNRGVTVLRR
                     VQGEHCEFMLVSLWDSMDAVRAFAGENPDKSVYYPEDDRYLLDMEPLVRHYEVAEQIP
                     EAVPEPKPAKKRK"
     gene            complement(1006..2055)
                     /locus_tag="VFF05_02905"
     CDS             complement(1006..2055)
                     /locus_tag="VFF05_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HZW50783.1"
                     /translation="MKFTVTLQASGKRFSVGDDESVLDAARRAGLALPYSCLSGVCGS
                     CKATLVSGDCHYPRNPPNALNAAEVAHHQVLLCQAVPRSDLVVVAREVASVAAMPKRV
                     LALKLIDKILLAPDVMRLRLAPPQGESLNRLPGQYLDVLLPGGKRRAFSIANAPHTGR
                     EIELHVRHVAGGDFTRRVFADLEPGAMLRVEGPLGTFVPREDSERPLLFVAGGTGFAP
                     VKALIEHFLHLGTRRAMTLYWGARAPQELYLRNLPEIWTTVAPSLRFVPVISETQSGN
                     DRRGFVHEAVLDDHPDLSGFDVYMSGPPALIDSGRRAFVAAGLPEDRLFYDSFDYAPD
                     VLAAILRGRAGIAEA"
     gene            2126..2557
                     /locus_tag="VFF05_02910"
     CDS             2126..2557
                     /locus_tag="VFF05_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HZW50784.1"
                     /translation="MPIWKTPVTLDRFNALNGDTMMQTIGIVFTEVGDDFLRATMPVD
                     ARTHQPYGLLHGGASVALAETLGSAAGMMLVDPAREIVVGIEINANHLRGVKSGIVTG
                     TARALHIGRSTQVWEIRIEDEGQRLVCISRLTLAVVPRPTA"
BASE COUNT          510 a          891 c          871 g          461 t
ORIGIN      
        1 gccgaacggc aagaagatcg acgccgacaa agccggggtg aacgtcacgg atcgcggctt
       61 cattgccgcc gacaagcaga tgcgcaccaa tgtgccgcac atcttcgcca tcggcgacat
      121 cgtcggcaat cccatgcttg cgcacaaggc cacgcacgag ggcaaggtcg cggctgaagt
      181 gtgcgcggga ctcaagagcg aattcgtcgc gcgcgtgatt ccgtcggtgg cctacaccga
      241 tccggaaatc gcctgggtcg gcctcaccga aggcgaggcg aaaaaagccg gcatcgccta
      301 cggcgtcggc aaattcccgc atgcggcatc ggggcgcgcc atcggcatcg cgcgcaccga
      361 aggtttcacc aaactcatct tcgacgaggc cacgcatcgc atcatcggcg ccggcgtcac
      421 ctgcccgaac gcgggcgagt tgatttccga actcgcgctc gccatcgaga tgggctgcga
      481 agccggcgac atcggcctga ccgtgcaccc gcatccgacc ctgtccgaat ccgtcggcat
      541 ggcggcggaa gtctatgaag gcacgatcac ggatttgtac attccgaagc gcaagtagct
      601 atttgcgctt cttcgccggc ttgggttcag gcacagcctc gggaatctgc tccgccacct
      661 cgtagtgccg caccagcggc tccatgtcga gcagatagcg gtcgtcttct ggatagtaga
      721 cggacttgtc gggattctcg ccggcgaaag cgcgcacggc gtccatcgaa tcccacagtg
      781 acaccagcat gaactcgcag tgctcgccct gcacgcggcg tagcacggtc acgccacgat
      841 tgccggacgt gttggcgtaa tccttcagcc ccgtttcctg caggtaggcc aggtaatcgt
      901 cggccttgtc ggcctgggtg ataccgcgcc agatgcgagc gatcatgcga ttctcccgct
      961 tgtgtcgcgc cagcataccc gagcacgcgc gtgagtgcgc gtggctcagg cttcggcgat
     1021 gccggcgcgt cctcgcagta tcgcggcgag tacgtccggc gcgtaatcga aggaatcgta
     1081 gaacaggcgg tcttccggca gtccggcggc gacgaaggcg cgacggcccg aatcgatcaa
     1141 cgccggcggt ccgctcatgt agacgtcgaa tccggacaag tccggatgat cgtcgagcac
     1201 cgcttcgtgg acgaaaccgc ggcggtcgtt gccggattgc gtttcggaaa tcaccggaac
     1261 gaaacgcagg ctcggcgcca ccgtcgtcca gatttccggc agattgcgca aataaagttc
     1321 ctgcggcgca cgcgcccccc agtacaacgt catcgcgcgg cgcgtgccca ggtgcaggaa
     1381 atgttcgatc agcgccttga ccggggcgaa tcccgtgcca ccggcgacga acagcagcgg
     1441 gcgttcggaa tcctcacgcg gcacgaaggt gccgagcgga ccttccacgc gcagcattgc
     1501 gccgggttcc agatcggcga atacgcgccg cgtgaaatcg ccgccggcaa cgtggcgcac
     1561 atgcagttcg atttcgcgtc cggtatgcgg tgcgttggcg atcgagaacg cgcggcgctt
     1621 gccgccgggc aacaacacgt cgagatattg cccgggcagg cggttcagcg attcgccttg
     1681 cggcggcgcc aggcgcaggc gcatcacgtc gggcgcgagc aagatcttgt cgatcagttt
     1741 cagcgcgagc acgcgcttgg gcatggccgc gaccgaagcc acctcgcgcg cgacgacgac
     1801 gagatcgctg cgcggcaccg cttggcacaa caacacttga tgatgcgcga cttccgccgc
     1861 gttcaacgcg ttcggaggat tgcgcgggta atggcaatcg ccggaaacca gcgtcgcctt
     1921 gcagcttccg cacacgccgg acaggcacga atacggcagc gccagtcccg cgcgccgcgc
     1981 ggcgtcgagc acgctctcgt cgtcgccgac gctaaagcgc ttgccgctgg cttgcagggt
     2041 gacggtgaat ttcacgcggc gattgtcgcc gaaatgcagt cgccgatgtt gctatgctcg
     2101 cgcgaaccaa tccgggagaa agctcatgcc gatctggaag acccccgtta ctctcgaccg
     2161 cttcaatgcg ctgaacggcg acacgatgat gcaaacgata ggcatcgtct tcaccgaggt
     2221 cggcgacgat ttcttgcgcg cgaccatgcc cgtggacgcg cgtacgcatc agccgtatgg
     2281 attgctgcac ggcggcgctt ccgtggcact cgccgaaacg ctcggcagcg cagccggcat
     2341 gatgctggtc gatccggcgc gggagatcgt ggtcggcatc gaaatcaacg ccaatcacct
     2401 gcgcggcgtg aaatccggaa tcgtcaccgg caccgcacgc gccctgcaca tcggccgcag
     2461 cacgcaggtc tgggaaatcc gcatcgagga cgagggccag cgcctggtct gcatttcgcg
     2521 gctgactttg gcggtggtgc cacgcccaac agcgtagggc gcaaaatatc gtaatgctgc
     2581 attgcggcga aaagcgcatg cggctaacat cgtggcgatg cattccacgg tcgaatccct
     2641 gtccccggcg cgccgcgact ggcggcggcc gctgcgctat ctgtggcgca cgccttgggt
     2701 attcgtccac gtcgtcgttg ccgtgccgct cac
//
LOCUS       DASRVO010000237         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_85332, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000237 DASRVO010000000
VERSION     DASRVO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85332"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..443)
                     /locus_tag="VFF05_02915"
     CDS             complement(105..443)
                     /locus_tag="VFF05_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZW50785.1"
                     /translation="MKMVMAIIKPFKLDDVREALAEAGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKLKLEIAVPDNLVERVVEAISKSANSGKIGDGKIFVYALDQVIRI
                     RTGETDADAL"
     gene            580..825
                     /locus_tag="VFF05_02920"
     CDS             580..825
                     /locus_tag="VFF05_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HZW50786.1"
                     /translation="MFNATAIDDLARRLADLVPPGAREARDDLAANFRDALRAGLRKL
                     DLVTREEFDVQRSVLLRTREKVEALEARVDELESKSG"
     gene            900..>1269
                     /locus_tag="VFF05_02925"
     CDS             900..>1269
                     /locus_tag="VFF05_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HZW50787.1"
                     /translation="MSLAIVHSRAQDGVSAPPVTVEVHLSGGLPGTNIVGLPETAVKE
                     ARDRVRVAIQNAQLEYPSRRVTVSLAPADLPKDGGRFDLPIALGILAGSGQFASELFQ
                     RYAVVGELALDGITRPTKGAL"
BASE COUNT          205 a          403 c          418 g          243 t
ORIGIN      
        1 cggaatggtc gcacctgaaa ctcgcggacg cgcggaaatt cactgccgcg ctgcacgcga
       61 tcgtggtgcg ggatttctgg aagccggatc cgccgaagta atccttacag cgcatccgca
      121 tcggtctcgc cggtgcgaat gcggatgacc tggtccagcg cgtagacgaa aatcttgccg
      181 tcgccgatct tgccgctgtt ggcggatttg ctgatcgcct cgaccacgcg ctcgacgagg
      241 ttgtccggca cggcgatttc gagcttgagc ttgggcagga aatcgaccac gtattcggcg
      301 ccgcggtaca gctcggtgtg gcccttctgc cggccgaaac ccttgacctc ggtcaccgtg
      361 atgccctgca cgcccgcctc cgccaatgct tcgcgcacat cgtcgagctt gaacggcttg
      421 atgatggcca tcaccatttt catggattgc tccgtcgggt cgcgggcgga cactataccc
      481 ggcgtgtagg cgatttccta cgcggcaaga cgtgcacagc cgacagcgcc ggcgtgcgtg
      541 tttggctaca ctgcgcgcat cgaattgcag gagcgcgcca tgttcaacgc caccgccatc
      601 gacgacctgg cccgccgcct ggccgatctg gttccgcccg gcgcgcgcga ggcgcgcgac
      661 gatctcgccg cgaacttccg cgacgcgctg cgcgcgggtc tgcgcaagct cgatctggtc
      721 acgcgcgagg aattcgacgt gcaacgctcc gtgttgctgc gtacgcgcga gaaagtcgag
      781 gcgctggaag cacgcgtgga cgagctggaa agcaagtccg gctgaccagt tcgctgattc
      841 cgattgacgg gtttgcgccg cgcacgcggc acgggcgggt gttgtccgga ggtttcttca
      901 tgagtctcgc catcgtccac tcccgcgccc aggacggcgt ttccgcaccg cccgtcacgg
      961 tggaagtgca tctgtccggt ggattgccgg gcaccaacat cgtcggtttg cccgagacgg
     1021 cggtgaagga agcgcgcgat cgcgtgcgtg tggcgatcca gaacgcacaa cttgaatatc
     1081 cctctcgccg cgtcacggtg agcctggcgc cggcggattt gccgaaagac ggtggccgct
     1141 tcgacctgcc catcgcgctc ggcattctgg cgggaagtgg acaatttgcg tcggagctgt
     1201 ttcagcggta tgcggtcgtc ggcgagttgg cgttggacgg catcacccgg ccgacgaaag
     1261 gcgcgttgt
//
LOCUS       DASRVO010000238         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_344601, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000238 DASRVO010000000
VERSION     DASRVO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344601"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1516)
                     /gene="gatA"
                     /locus_tag="VFF05_02930"
     CDS             complement(77..1516)
                     /gene="gatA"
                     /locus_tag="VFF05_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZW50788.1"
                     /translation="MADIAQLAQALRARKVSAIELARQSLATARSRSDLNAFVTLDED
                     AALAAAQRADAALAAGAAPALAGIPFVHKDIFCTNGIRTTCASRMLQNFVPPYDAAVV
                     ERLALAQAVSIGKANMDEFAMGSSNENSCFGAVKNPWDVTRVPGGSSGGSAAAVAAGI
                     VPLATGTDTGGSIRQPAAFCGITGLKPTYGRVSRYGMIAYASSLDCAGVLARSAADCA
                     AVFAAMAGHDARDATSVDRPCDDCVGALSTKLQGIKIGVAREYFGAGVEAGVDAATRA
                     ALREFERLGATLVDISLPHAALAIPAYYVIAPAEASSNLARYDGVRYGHRCTDPQSLE
                     DLYTRSRGEAFGAEVKRRILIGTYALSAGYYDAYYLRAQRARRVVADDFAEAFKTVDL
                     IAGPTAPTVAFKLGEKTSDPLAMYAADVNTVAVNLAGLPAISIPAGFSDSLPVGLQLI
                     APAFAESALLAAAHQFQLATDWHARSPNA"
     gene            complement(1528..1815)
                     /gene="gatC"
                     /locus_tag="VFF05_02935"
     CDS             complement(1528..1815)
                     /gene="gatC"
                     /locus_tag="VFF05_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZW50789.1"
                     /translation="MAIDSATVARIARLARLPLDARTSTQTANELARVLDLAGQLANA
                     ALDGVEPMAHPHAQTLVWRADAVTESDRADALLAQAPDARAGLYAVPKVIG"
     gene            1990..>2384
                     /locus_tag="VFF05_02940"
     CDS             1990..>2384
                     /locus_tag="VFF05_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZW50790.1"
                     /translation="MFGLGRFFSNDLSIDLGTANTLIYVRGQGIVLNEPSVVAIRQDR
                     GPGGPRAVAAVGGDAKRMLGRTPGNIVTVRPLKDGVIADFSMTEEMLKQFIKKVHDNK
                     LFSPSPRIIICVPCGSTQVERRAIRESALG"
BASE COUNT          422 a          803 c          774 g          385 t
ORIGIN      
        1 gtggacgcgc cggaaaacag tttcgtccgc gtcgacaact gcgcgtggat ttccaggccg
       61 atgacaggct gccagctcat gcgttcggac tccgcgcatg ccagtcggtg gcaagctgga
      121 actgatgcgc agccgcaagc aacgcgcttt cggcgaacgc cggcgcgatc agctgcaagc
      181 cgaccggcag actgtcgctg aaacccgcag gaatcgagat cgccggcagt cccgcgagat
      241 tcaccgccac ggtgttgaca tcggcggcgt acatggcgag cggatcggac gtcttctcgc
      301 cgagtttgaa ggcgacggtc ggtgcggtcg gacccgcgat caggtcgacc gtcttgaacg
      361 cttcggcgaa gtcgtccgcg accacgcgcc gcgcgcgctg cgcgcgcaga tagtacgcgt
      421 cgtagtaacc cgccgacaac gcataggtgc cgatgaggat gcgacgcttg acctcagcgc
      481 caaaggcctc gccacggctg cgcgtgtaca aatcctcgag cgactgcgga tccgtgcagc
      541 gatggccgta acgcacgcca tcgtagcgcg cgaggttgct cgaagcctcg gccggcgcaa
      601 tcacgtaata cgccggaatc gcgagcgccg catgcggcag ggaaatatcg accagtgtcg
      661 cgccgagtct ttcgaattcg cgcagggctg cgcgcgtcgc cgcgtcgaca ccggcctcga
      721 cccccgcgcc gaaatattcc cgcgcgacgc caatttttat accttgcagc tttgtagaca
      781 atgcgccaac gcagtcgtcg caagggcgat ccacactcgt cgcatcgcgc gcatcgtgac
      841 cggccatcgc cgcgaacacc gccgcgcaat cggccgcgct gcgtgcgagt acgccggcgc
      901 aatccaggct cgacgcatac gcgatcatgc cgtagcgcga aacgcggccg taggttggtt
      961 tcagtccggt gatgccgcag aacgccgctg gctggcggat cgatccgccg gtatcggtgc
     1021 cggtcgctaa cggcacgatg ccggcggcga ccgctgctgc cgatccgccg gacgatccgc
     1081 cgggcacacg cgtcacgtcc cagggattct tcaccgcgcc gaagcacgaa ttctcgttcg
     1141 acgagcccat cgcgaactcg tccatgttcg ccttgccgat ggacaccgcc tgcgccaggg
     1201 cgagcctttc caccacggcc gcatcgtagg gcggcacgaa attttgcagc atgcgcgagg
     1261 cgcaggtcgt gcggattcca tttgtgcaaa aaatatcctt gtgcacaaag ggaatcccgg
     1321 ccagtgccgg cgccgcaccc gccgcgaggg cggcgtcggc gcgttgcgca gcggcgagcg
     1381 cggcgtcctc gtcgagcgtg acgaaggcgt tgaggtcgga acgcgaccgc gccgtcgcca
     1441 gcgactgccg cgccaactcg atggcggaga ctttacgtgc gcgcagggcc tgcgcgagtt
     1501 gcgcgatatc cgccatcgtt gccgaaccta gccaatgacc ttgggcacgg catacaggcc
     1561 ggcgcgcgcg tccggcgcct gcgcgagcaa ggcatcggcg cggtcggatt cggtgaccgc
     1621 atccgcgcgc cagaccagcg tttgcgcgtg cggatgcgcc atcggttcga cgccgtcgag
     1681 ggccgcattc gccaactgcc cggcgagatc gagcacgcgc gccagctcgt tcgcggtttg
     1741 cgtcgatgtg cgcgcatcca gcggcaggcg tgcgagccgg gcgatgcggg cgacagtggc
     1801 ggagtcgatg gccatggacg atccggagcg ttgacgagcg acgataacac ggcgcgatcg
     1861 tcgcgacgaa gcgcgaaaaa cgtgccgcaa gccgcatttt aacggcattt ttccttgctt
     1921 gtgcggcgcc gcgaatctac actgtcgcgc ttttcgcgat tccgcgaaaa atccatcaca
     1981 gagcgcttca tgttcggctt aggcagattc ttttccaacg acctgtcgat cgatctcggc
     2041 acggcaaaca ccctgattta cgtgcgcggc cagggcatcg tcctgaacga gccttccgtc
     2101 gtcgccatcc gccaggaccg cggcccgggc ggcccgcgtg cggtcgcggc cgtcggcggc
     2161 gacgccaagc gcatgctcgg gcgcaccccc ggcaacatcg tcaccgtgcg tccgctcaag
     2221 gacggcgtga tcgccgactt ctcgatgacc gaggagatgc tcaagcagtt catcaagaag
     2281 gtgcacgaca acaagctgtt ctcaccgagc ccgcgcatca tcatctgcgt gccgtgcggc
     2341 tcgacccagg tcgagcgccg cgcgatccgc gagtcggcac tcgg
//
LOCUS       DASRVO010000239         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_267187, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000239 DASRVO010000000
VERSION     DASRVO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267187"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFF05_02945"
     CDS             <1..881
                     /locus_tag="VFF05_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exosporium glycoprotein BclB-related protein"
                     /protein_id="HZW50791.1"
                     /translation="DANGNLTIANLATAAQQGTPLCFSGTGALGPCVPGSNIGPTGPT
                     GPTGPTGAAGPTGATGLQGTQGPTGATGPTGLTGATGPTGAQGIQGPTGATGPTGATG
                     PTGQTGPMGATGATGPAGSGTIIPFASGRDYTATTSFSGLPGTVVLLGFGNGIDGVSP
                     GGAIIDLSGSPGSTMNFAFSMPRDGTITSISVYFSTTVAQSLIGTTISLTGQLYQSTT
                     PDNIFTPIPGAMVTLAPALTGVLTMGTVSNGLTTGLSIPVTAQTRLLFVGSASAAGIS
                     LIDSVPGYWSAGVTIQ"
     gene            925..>1906
                     /locus_tag="VFF05_02950"
     CDS             925..>1906
                     /locus_tag="VFF05_02950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW50792.1"
                     /translation="MIVKNEAPVIERCLRSVKPFVHAWAIADTGSSDGTQDIVRRFMA
                     DLPGELIERPWVDFSTNRNEALELARKYGDYALIIDADDTLEADSGFAWPELGAGGYV
                     LEVVDTGNIHYQRIAIPRLDAGWEWRGVLHEALFAPAAVSTPLVSGLRILRLHNDGAR
                     SRQSQIEKFSHDAQVLRRALEKEPDNARYRFYLAQSLRDAGNLPGAIAEYEKRAAMGG
                     WQEEVYFSRFQIGVLKERNAAPYAEVVAAYLDAFNSRPSRAEAPCELARYLRINQRWN
                     LARHFAQIAASLPVPDDVLFIDRAVHDWRARDELSIATFWCGDYAQCAALC"
BASE COUNT          346 a          641 c          596 g          323 t
ORIGIN      
        1 tcgatgcgaa cggcaacctg accattgcca atctcgccac cgccgcgcag cagggcacgc
       61 cgctgtgctt ttccggaacc ggagcgctcg gcccttgcgt gcctggttcc aatatcggcc
      121 cgacgggacc gaccggccca acgggcccca cgggtgccgc cggcccgacg ggcgcgactg
      181 gcttgcaagg cactcaaggc ccgacaggcg ctaccggccc gacaggcttg acgggtgcca
      241 cgggtccgac cggcgcacaa ggcattcaag gcccgactgg tgcgaccggt ccgaccggcg
      301 cgacgggacc gacaggccag accggtccga tgggcgcgac gggagcgact ggcccggcgg
      361 gatcaggcac catcattccc ttcgcatccg gccgcgacta cacggcaacc acctcattca
      421 gcggactgcc gggcaccgtt gtcttgctgg gtttcggcaa tggtatcgac ggcgtcagtc
      481 cgggcggcgc aatcatcgac ctgtcgggga gcccaggcag cacgatgaat ttcgccttca
      541 gcatgccgcg cgacggcacg atcacatcga tttcggtcta cttcagcacg accgttgcgc
      601 aatccctgat cggcacgacg atctccctga ccgggcaact ctaccaatcg acaacgccag
      661 acaacatatt cacgccgatt cccggagcca tggtgacact tgcgcctgcg ttgaccggag
      721 tgctgacgat gggaactgtc tcgaacggcc tcacgaccgg gctttccatt ccggtgacgg
      781 cgcagacacg attgctgttc gtgggttccg cgtccgccgc cggcatctcg ctcatcgaca
      841 gcgtgcccgg ctattggagt gccggcgtaa cgattcaata gcgctgcgga caggcataat
      901 tcgcatatgc ggatatgcct gtccatgatc gtcaagaacg aggcgccggt gatcgaacgg
      961 tgcctgcgct cggtcaagcc attcgttcat gcctgggcga tcgccgatac cggttcgagc
     1021 gacggcaccc aagacatcgt gcgccggttc atggcggacc tgccgggcga gttgatcgaa
     1081 cggccgtggg tggatttctc cacgaaccgg aacgaagcgc tggaactcgc gaggaaatac
     1141 ggcgactacg cgctgatcat cgacgccgac gacactctcg aagccgattc cggcttcgct
     1201 tggccagaac tcggcgctgg cggctatgtg ctggaggtcg tagacaccgg caacatccac
     1261 tatcagcgca ttgccatacc gcgcctcgac gcgggctggg aatggcgcgg cgtgctgcac
     1321 gaagccttgt tcgcgcctgc ggccgtgtca acgccgttgg tttccggact gcgcatcctg
     1381 cgcctccaca acgacggcgc gcgcagccgc cagtcgcaga tcgagaagtt cagccacgac
     1441 gcgcaagtgc tgcgccgcgc gctcgaaaaa gaaccggaca acgcgcgcta ccggttttac
     1501 ctggcacaga gcctgcgcga cgcgggcaac ctgcccggcg cgatcgccga atatgaaaag
     1561 cgcgcggcga tgggcggttg gcaagaagaa gtatactttt cccgattcca gatcggcgtg
     1621 ctgaaggaac gtaacgctgc gccgtatgcc gaggtcgtcg ccgcctatct ggacgccttc
     1681 aattcccgcc ccagtcgcgc ggaagcgccg tgcgagctgg cgcgctattt gcgcatcaac
     1741 cagcgctgga atctcgcccg ccatttcgcc cagattgccg cgtcgctgcc ggttcccgac
     1801 gacgtgctgt tcatcgaccg cgccgtacac gattggcgcg cgcgcgacga gctgtcgatc
     1861 gccacgttct ggtgcggcga ttacgcacaa tgcgcggcgc tgtgcc
//
LOCUS       DASRVO010000240         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_240667, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000240 DASRVO010000000
VERSION     DASRVO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240667"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFF05_02955"
     CDS             complement(<1..325)
                     /locus_tag="VFF05_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium transporter"
                     /protein_id="HZW50793.1"
                     /translation="MSHLSALDYLVVVGYFLVTAAIGYSVARTKTTSEDLFLASRSLG
                     PVAVGFSLFAANVSSDTLIGLPGAAYRNGIAAANYEWMAGAVLIFTAFCVLPVLMRAR
                     VTTMPE"
     gene            464..949
                     /locus_tag="VFF05_02960"
                     /pseudo
     CDS             464..949
                     /locus_tag="VFF05_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660137.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
BASE COUNT          207 a          324 c          344 g          179 t
ORIGIN      
        1 attcgggcat ggtcgtgacc cgcgcacgca tcaggacggg caacacgcaa aaggcggtga
       61 agatgagtac cgcgccggcc atccattcat aattggctgc ggcaatcccg ttccggtaag
      121 ccgcgccggg caggccgatc agggtgtcgg aagacacatt cgccgcgaac agcgaaaatc
      181 ccaccgccac gggtccgagt gagcggctgg cgagaaaaag gtcttcggag gtggttttgg
      241 tgcgcgctac cgaatagcct atcgcggcgg ttacgaggaa gtagccaacg acgacaaggt
      301 agtcaagcgc actgagatgg gacattggac tcccgtcggt gcgattccat ttcaggaaaa
      361 cacgtaccgg gcgaggatgc aagcccgatg cgcggcttcc atgctaggat tcggcccctt
      421 ttgcaaaatt gacgcactgc aagattgtgc ggagacggga ttgatgcgta accatttttg
      481 cggccttgtc gatgaggcct tgattggtcg tgacgtcact ctgtgcggct gggccgacac
      541 ccggcgcgat cacggcggcg tgatcttcat cgacctgcgc gaccacgagg gcatcgtcca
      601 agtggtgatc gagccggaca acgcgcccgc gttcgccgcg gctgagcatg tgcgctacga
      661 atattgcctg cgcatcgtcg gcaaggtgcg ccatcgcccg gcagcccagg tcaacgaacg
      721 cctgcgcacc ggcaagatcg aagtcgtcgc cgcgacggtc gaggtgctca acgccgccgc
      781 gcccctgccg ttcatgctgg acgacgccac cggcgaggag ccgcgcctgc gctggcgtta
      841 cctcgacctg cgccgtccgg aaatgcagcg caagatgcgc ctgcgcacga aactcgtcgc
      901 ggcgttgcgc agatatctgg atgcgcgcgg cttccaggac ctggaaaccg cgcgcatcca
      961 gatatctgcg caacgccgcg acgagtttcg tgcgcaggcg catcttgcgc tgcatttccg
     1021 gacggcgcag gtcgaggtaa cgccagcgca ggcg
//
LOCUS       DASRVO010000241         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_319165, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000241 DASRVO010000000
VERSION     DASRVO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319165"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="purT"
                     /locus_tag="VFF05_02965"
     CDS             complement(<1..1022)
                     /gene="purT"
                     /locus_tag="VFF05_02965"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HZW50794.1"
                     /translation="MKPFGTPHTDTAIRVLLLGSGELGKEVAIELQRFAVEVIAVDRY
                     AHAPAMQVAHRSHVINMLDGAEIRRVIALEKPDLVVPEIEAIHTPTLVELEEQGLTVI
                     PTARAAWLTMDREGIRRLAAEELKLPTSPYRFVDTVDEYRAAIAAIGYPFVIKPVMSS
                     SGKGQSTVRRAEEADAAWEYAQSGGRAGKGRCIVEGFVDFDYEITQLTVRHAGGTSFC
                     APIGHRQEAGDYRESWQPQAMSKAALIEAQRQARAITDALGGRGVFGVEFFVKGDAVI
                     FSEVSPRPHDTGLVTLISQDLSEFALHARAILGLPIPAIRQLGPSASTAVLCHGKGTR
                     PVYHGVA"
BASE COUNT          199 a          399 c          416 g          214 t
ORIGIN      
        1 gcgacgccgt gatagaccgg tcgtgtgccc ttgccgtggc acaacaccgc ggtcgacgcg
       61 gatggcccga gctggcggat ggcggggatc ggcaggccga ggatcgcgcg cgcgtgcagc
      121 gcaaattcgg acaggtcctg cgagatgagg gtgaccagtc ccgtgtcgtg cgggcgcggg
      181 ctgacttcgg aaaagatcac cgcgtcgccc ttgacgaaga attccacgcc aaacacgccg
      241 cgtccgccaa gcgcatcggt aatcgcgcgc gcctggcgtt gcgcttcgat caatgccgcc
      301 ttgctcatcg cctgcggttg ccaggattca cgatagtcgc cggcttcctg gcgatgtccg
      361 atcggggcgc agaacgacgt gccgccggcg tggcgcacgg tcaactgggt gatttcgtag
      421 tcgaagtcga cgaaaccctc gacgatgcag cgccccttgc cggcgcgtcc accggattgc
      481 gcgtattccc aggcggcatc cgcctcttcc gctcgacgca ccgtgctctg gcccttgccc
      541 gatgaactca tcaccggctt gatcacgaac ggatagccga tagcggcaat cgcggcgcga
      601 tattcgtcta ccgtgtcgac gaagcggtag ggcgaagtcg gcagtttcaa ttcctcggcg
      661 gcgaggcggc ggatgccttc gcgatccatc gtcagccacg cggcacgcgc ggtgggaatg
      721 acggtcaggc cctgttcttc cagttcgacc agggtcggcg tgtggatcgc ttcgatctcg
      781 ggcacgacca gatcgggttt ttccagcgcg atgacgcggc ggatttccgc gccgtccagc
      841 atgttgatga cgtggctgcg atgcgcaacc tgcatggccg gcgcgtgcgc gtagcggtcg
      901 accgcgatca cctcgacggc gaagcgctgc aactcgatcg ccacttcctt gccgagctcg
      961 ccggaaccga gcaacaggac gcggatcgcg gtgtcggtat gcggggtgcc gaagggtttc
     1021 acgcgcgggc tccgggcgga cggggcgccg attatgccac ccgcgtgcgc cgtcaaggcg
     1081 cggtgtgacg tcaacgcgac ctgcgcgggt gccggccaat gcacgacccg caccggagat
     1141 ttgggtcggg cgtgcaaggc cgaaacgccg gtctaggttc gccaggcttg cgcatctccc
     1201 ctagcgaaaa tccctgctcc tcgcatcg
//
LOCUS       DASRVO010000242         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_85452, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000242 DASRVO010000000
VERSION     DASRVO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85452"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..>2005)
                     /locus_tag="VFF05_02970"
     CDS             complement(123..>2005)
                     /locus_tag="VFF05_02970"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HZW50795.1"
                     /translation="TNPIGPAQLCQGFRIAMRELELNLQTQLIVYKLFDRYVLAGLDP
                     LYEEIDAQLIQAGVLPQIRHVVGSGRGLRPPAAPQVPGAAAPTDGDAPAEAGQAAYPV
                     QYDAAAAQLQAELYNTVRGLLANRRANLPQSEGAADFAPGMAVPYIAPTDLLSALTLL
                     QNQTWAAQSSASSAADAAQAVQQIKQALLDQAGKLHGNDKAHVAGADEDTIDLVGMLF
                     EYILQDRNLPAQMQALLGRLQIPFLKAAILDKHLFAQKSHPARQLLDAMAQACIGWSE
                     ESDKDHRLYDKVKQTVEVLLKDFDDDLAIFEKERVDFETFLDSNRKRADLAEQRAAEA
                     TRGREKLHSARRISAREILQRVEGKEMPEIVRTVLTRPWANYLVLTLLRQGEDSNEWR
                     QALRFADEFVWSAQPKTSDAERMRLKGILPALEKSLRHGLATVAYHENDVKQLMHELN
                     AFYTNLLNGDTPAAAEVAKVQSEAAPGFEIVGTATATAAAIDSPVEEEIVLRAEAATA
                     PELGDEDESLRQVKAMKVGTWIEFTGVDGTTKERAKLSWISPISSKYLFVNRKGLKVA
                     DKTIQALAIEIRRGDAAILEEVPLFDRALDAIVERLQASNHGRPHTTAASRAITRACA
                     CA"
BASE COUNT          318 a          639 c          655 g          393 t
ORIGIN      
        1 agcgtgagga tgcgcgagcc gatgtcgcaa ccgccggatt ccgcgacggt gctagccgac
       61 gtgcgccgcg cgctcgccga ggatttcggc agtggcgacg tcaccgccga tctgttgcag
      121 cctcaagcgc aggcgcacgc ccgcgtgatc gcgcgcgaag cggccgtggt gtgcggacgg
      181 ccgtggttcg atgcctgtag gcgttcgacg atcgcgtcga gggcgcggtc gaacaacggc
      241 acttcttcca ggatcgccgc gtcaccgcgg cggatttcga tggccagcgc ctgtatggtc
      301 ttgtccgcca ccttcagacc cttgcgattc acaaacaggt atttggagct gatcgggctg
      361 atccacgaca gcttcgcgcg ttccttggtc gtgccgtcga caccagtgaa ttcgatccag
      421 gtgccgactt tcatcgcctt cacctggcgc aggctttcgt cctcgtcgcc gagttcgggt
      481 gcagtcgcgg cttctgcgcg caggacgatt tcttcctcga ccgggctgtc gatcgccgcc
      541 gcggtcgcgg tagcggtgcc gacgatctcg aatcccggcg ccgcctcaga ttggaccttc
      601 gcgacttcgg cggcagccgg cgtatcgcca ttgagcaggt tcgtatagaa cgcgttgagc
      661 tcgtgcatca actgcttgac gtcgttctcg tggtacgcga cggtggccaa gccgtggcgc
      721 aaggattttt ccagcgcggg caggatgccc ttcaaacgca tgcgctcggc atcgctggtt
      781 ttgggctgcg cgctccagac gaattcatcg gcgaagcgca aggcctggcg ccattcgttg
      841 gaatcctcgc cctggcgcaa cagcgtaagc accaggtaat tcgcccacgg ccgcgtcagc
      901 accgtgcgca cgatctcggg catctccttg ccctcgacgc gctgcaggat ttcgcgcgcc
      961 gaaatgcggc gtgccgagtg cagtttttcg cggccgcgcg tggcctctgc agcgcgttgc
     1021 tcggccagat ccgcgcgctt gcggttggaa tcgaggaacg tctcgaaatc gacgcgctcc
     1081 ttctcgaaga tggccagatc gtcgtcgaaa tccttgagca ggacttccac ggtctgcttg
     1141 accttgtcgt agagacgatg atccttgtcg gattcctccg accagcctat gcaggcctgc
     1201 gccatggcgt cgagcagttg gcgtgccgga tgcgatttct gcgcgaacag gtgcttgtcg
     1261 agaatcgcgg ccttgaggaa gggaatctgc aagcgcccga gcagcgcctg catctgcgcc
     1321 ggcaggttgc ggtcctgcag gatgtactcg aacaacatgc cgacgaggtc gatggtgtcc
     1381 tcgtcggcgc cggcgacatg cgccttgtcg ttaccgtgca gtttgccggc ctggtcgagc
     1441 agggcctgct tgatctgctg cacggcctgg gctgcgtcgg ccgccgaaga cgcactgctc
     1501 tgcgccgccc aagtctggtt ctgcagcaag gtcagcgcgg acaacagatc ggtcggcgcg
     1561 atgtacggca cggccatgcc cggcgcgaaa tcggcagcgc cctcgctttg cggcaaattg
     1621 gcgcggcgat tcgcgagcag tccgcgcacc gtgttgtaca actcggcctg aagttgcgcg
     1681 gcggccgcat cgtattggac cggataggcg gcctgacccg cttcggcagg cgcgtcgcca
     1741 tcggtcggcg ccgctgcacc cggaacctgc ggcgctgcgg gcgggcgaag accgcgaccg
     1801 ctaccgacga cgtgacgtat ctgcggcagc acgccggcct ggatcagctg cgcgtcgatt
     1861 tcttcataaa gcggatccag tccggcgagc acgtaacgat cgaacagctt gtagacgatc
     1921 aattgcgtct gcaggttcaa ttccagttcg cgcatggcga tgcggaaccc ctggcacaat
     1981 tgcgccggcc cgatcggatt ggtcg
//
LOCUS       DASRVO010000243         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_370641, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000243 DASRVO010000000
VERSION     DASRVO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370641"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /gene="secG"
                     /locus_tag="VFF05_02975"
     CDS             <1..421
                     /gene="secG"
                     /locus_tag="VFF05_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZW50796.1"
                     /translation="LVAIAMCVLILLQRGDGAAAGSGFGGGASATVFGARGATTFLSR
                     STAILAAVFFILSIAMGIYLHPVANPNSDLGIMSGVGTSEPAKAPAPVAVPAAPVKND
                     VPAIPAKSDVPAATPAPVAAPAAEKKEGGKPEATKKQ"
     gene            433..517
                     /locus_tag="VFF05_02980"
     tRNA            433..517
                     /locus_tag="VFF05_02980"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:467..469,aa:Leu,seq:gag)
     gene            628..984
                     /gene="ndhC"
                     /locus_tag="VFF05_02985"
     CDS             628..984
                     /gene="ndhC"
                     /locus_tag="VFF05_02985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZW50797.1"
                     /translation="MLAEYLPILLFLIVAGGIGVALLGIGALLGPRSANKEKSDAYEC
                     GFEAFEDARMKFDVRYYLIAILFIIFDLEIAFLFPWAVVFKHIGIVALVEMLMFLVLL
                     LLGFVYCWKKGALEWE"
     gene            981..1559
                     /locus_tag="VFF05_02990"
     CDS             981..1559
                     /locus_tag="VFF05_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZW50798.1"
                     /translation="MTDTTVAKSLFHNPVPTSPVDDILRPDGDNPLLQRGWATASMDA
                     LVNWARTGSMWPMTFGLACCAVEMMHAGAARIDLDRYGVVFRPSPRQSDVMIVAGTLV
                     NKMAPALRRVYDQMPNPKWVISMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPP
                     TAEALVYGILQLQKKIRRTETVGRKSDKVRIA"
     gene            1562..2278
                     /locus_tag="VFF05_02995"
     CDS             1562..2278
                     /locus_tag="VFF05_02995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZW50799.1"
                     /translation="MDTLAQRVATQLGDKIAATHEWRGETIVDVLPENWLAVAQHLRD
                     EAEFRFEQFIDLCGVDYLSYGQVEWDTTDVTSTGFSRGVEGEGPGRFDWQERPHPGIG
                     NKRFGVIVQLTSIAHNRRVRLRCHAQDDAFPVVPSLVPLWPGANWFEREAFDLLGIVF
                     EGHPDLRRILTDYGFVGHPFRKDFPLIGNVEVRYDEAQGRVIYEPVTSVVPRVLVPRV
                     IRQDSRWEQAQAEAAGEAKK"
     gene            2281..>2667
                     /locus_tag="VFF05_03000"
     CDS             2281..>2667
                     /locus_tag="VFF05_03000"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466202.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZW50800.1"
                     /translation="MQEIRNYTINFGPQHPAAHGVLRLVLEMDGETVVRADPHVGLLH
                     RGTEKLAESKPFNQSIGYMDRLDYVSMMCNEHAYVRAIEKLMGIEAPLRAQYIRTLFD
                     EITRILNHLMWIGSNALDLGAMAVFLY"
BASE COUNT          470 a          833 c          863 g          501 t
ORIGIN      
        1 gctcgtcgcg atcgcgatgt gcgtgctcat cctcttgcag cgcggcgacg gcgcggcggc
       61 cggttcgggt ttcggcggcg gcgcgtcggc gacggtgttc ggcgcgcgcg gcgcgaccac
      121 gttcctgtcg cgcagcacgg cgatcctggc ggcggtcttt ttcatcctca gcatcgccat
      181 gggcatttac ctgcatcccg tcgcgaaccc gaacagcgat ctgggtatca tgagcggcgt
      241 cggtacgtcc gaacccgcca aggcgccagc acccgtcgcg gtgcccgcag cgccggtgaa
      301 gaacgacgtc ccggcaattc cggcaaagag cgatgtgccg gccgcgacgc cggctccggt
      361 tgcggcgcct gctgcggaaa agaaagaagg cggcaaaccg gaagctacaa agaaacagta
      421 atcattctct ttgcccaggt ggcggaattg gtagacgcac taccttgagg tggtagcgcc
      481 gaaaggcgtg gaggttcgag tcctctcttg ggcaccaaca aacaaacccg cgcgagcggg
      541 tttttttgtc gcttcgcatt gcgtgtgcaa ccgcgcgcgt gcgaaaatcg gcaggctttg
      601 ctgacctgcc atgaggtgac tcgtcccgtg ctggccgaat acctgccgat actcctgttc
      661 ctgatcgtcg ccggcggcat cggcgtggcc ttgctcggca tcggcgcact gctcgggccg
      721 cgcagcgcga acaaggaaaa atccgacgcc tacgaatgcg gtttcgaagc gttcgaggac
      781 gcgcgcatga aattcgacgt gcgctattac ctcatcgcca tcctgttcat cattttcgat
      841 ctggaaatcg ccttcctgtt tccgtgggcg gtggtgttca agcacatcgg catcgtcgcg
      901 ctggtcgaga tgctgatgtt cctggtgttg ctgcttttgg gatttgtgta ctgctggaag
      961 aaaggggctt tggaatggga atgaccgaca ccaccgtcgc gaagagcctt ttccacaacc
     1021 cggtgccgac cagcccggtc gatgacatcc tgcgtccgga cggcgacaac ccgctgctgc
     1081 agcgcggctg ggcgaccgcg tcgatggacg cgctggtcaa ctgggcacgt accggctcga
     1141 tgtggccgat gactttcggc ctggcctgct gcgcggtgga aatgatgcac gccggcgccg
     1201 cgcgcatcga cctggatcgc tacggcgtgg tgttccggcc aagtccgcgc cagtccgacg
     1261 tgatgatcgt ggccggcacc ctggtcaaca agatggcgcc ggcgctgcgc cgcgtctacg
     1321 accagatgcc gaatccgaag tgggtcatct cgatgggctc gtgcgcgaac ggcggcggct
     1381 actatcacta ttcctatgcc gtggtgcgcg gctgcgatcg catcgtgccg gtcgacgtct
     1441 acgtgccggg ttgtccgccg accgcagaag cgctggtcta cggcatcctg caactgcaga
     1501 agaaaatccg ccgcaccgaa acggtcggca ggaaatccga caaggtgcgc atcgcatgag
     1561 catggacacg ctcgcgcagc gcgtcgccac gcaactcggc gacaagattg ctgcgacgca
     1621 cgagtggcgc ggcgagacca tcgtcgacgt gctgccggag aactggctcg ccgtcgcgca
     1681 gcatttgcgc gacgaagccg aattccgctt cgaacagttc atcgatcttt gcggcgtgga
     1741 ttacctgagc tacggtcagg ttgaatggga caccaccgac gtcacctcga ccggtttttc
     1801 gcgcggcgtg gaaggcgagg gcccgggccg cttcgattgg caggaacgtc cccatcccgg
     1861 catcggcaac aaacgcttcg gcgtgatcgt gcagctgacc tcgatcgcgc acaaccggcg
     1921 tgtgcgcctg cgctgccatg cgcaggacga cgcgtttccc gtggtgccgt cgctggtgcc
     1981 gttgtggccc ggcgcaaact ggttcgagcg tgaggcgttc gacctgctcg gcatcgtgtt
     2041 cgaagggcat ccggacctgc gtcgcatcct caccgactac ggtttcgtcg gtcatccgtt
     2101 ccgcaaggat ttcccgctga tcggaaatgt tgaagtgcgc tacgacgagg cgcaaggccg
     2161 cgtgatctac gaacccgtga ccagcgtcgt gccgcgtgtg ctggtgccgc gcgtcatccg
     2221 ccaggactcg cgctgggagc aggcgcaggc ggaagccgcc ggcgaggcga agaaatagcc
     2281 atgcaagaga tacgcaatta cacgatcaac ttcggcccgc agcatccggc ggcgcatggc
     2341 gtgctgcgcc tggtgctgga gatggacggc gagaccgtgg tgcgtgccga cccgcatgtt
     2401 ggcctgctgc accgcggtac cgaaaagctg gccgagtcca agccgttcaa ccagtcgatc
     2461 ggctacatgg atcgcctcga ctacgtctcg atgatgtgca acgagcacgc ctacgtgcgc
     2521 gccatcgaaa agctgatggg tatcgaggcg ccgctgcgcg cgcaatatat ccgcaccctg
     2581 ttcgacgaaa tcacgcgcat cctcaatcac ttgatgtgga tcggctcgaa cgcgctcgac
     2641 ctcggtgcca tggcggtgtt cctgtac
//
LOCUS       DASRVO010000244         1900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_33832, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000244 DASRVO010000000
VERSION     DASRVO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33832"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="VFF05_03005"
     CDS             <1..121
                     /locus_tag="VFF05_03005"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZW50801.1"
                     /translation="DQQTSTLMFTIEVRNRKHLADVMRGVRNAAVVHGVYRYP"
     gene            284..667
                     /locus_tag="VFF05_03010"
     CDS             284..667
                     /locus_tag="VFF05_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HZW50802.1"
                     /translation="MSRQIIASPDAPKAIGPYSQAVRCGNALYFSGQTPIDPATGVLV
                     PGDITAQARRVFENLKAVAAAAGADFARVARVGIYLTDLSNFAAVNAVMKEYFAEPFP
                     ARSTIGVASLPLNAQVEIDMIAVLD"
     gene            684..>1900
                     /locus_tag="VFF05_03015"
     CDS             684..>1900
                     /locus_tag="VFF05_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZW50803.1"
                     /translation="MRELVVPSDAALTTLPGVGPALAKTLERLGLRTLQDLWFHLPLR
                     YEDRTRLTPIRDLVPGAPAQVEGRVEAVERGFRYRPQLRVAVGDDSRATLLLRFFHFN
                     SAQANQLVVGTRLRCYGEVRSGAQGLEMVHPQYRRVADAETAVVEECLTPVYPVTEGL
                     TSTRLGGVIARALQRLSTDAELELLPPDLRKTHKLSSLRAALLYVHRPPPNADLAQLA
                     LGAHPAQQRLAFEELLAQHLSLKRLRARIREHAAPALRGAGDLRKRFLAGLPFAPTHA
                     QARVAAEVARDLGKPAPMLRLVQGDVGSGKTVVAALAALAAVESDSQAALMAPTELLA
                     EQHYRNFQRWMEPLGIEVVWLAGKLKGRVRSAALAKIASSAQIVIGTHALMQEGVAFR
                     DLALVIIDEQHRFG"
BASE COUNT          286 a          644 c          614 g          356 t
ORIGIN      
        1 cgaccagcaa acctcgacct tgatgttcac gatcgaggtg cgcaaccgca agcacctggc
       61 cgacgtcatg cgcggcgtgc gcaatgcggc ggtggtgcat ggcgtttacc ggtatccttg
      121 atgaaccgct gaataagctg ctgtgctcgg caggctgcgc gccggcggtg ctcggaatgc
      181 tcatgtacta gcgtgtacac tccgcttcct gcgctccggc gtcgcgcatt ccgcgttcgc
      241 tcgcgacgct tcttcagcga ttcctcgtat ttggagttct cgcatgtccc gccagattat
      301 cgcttcaccc gacgcaccca aggccatcgg cccgtattcg caggccgtgc gctgcggcaa
      361 cgccttgtat ttttccggac agacgccgat cgacccggcc accggcgtgc tggtgccggg
      421 cgacatcacg gcgcaggcgc ggcgtgtttt cgagaatctc aaggccgtcg ccgcagcggc
      481 cggtgcggac ttcgcgcggg tcgcacgcgt gggcatctat ctcaccgatc tttccaactt
      541 cgccgctgtc aacgcggtga tgaaggaata tttcgccgaa cctttcccag cgcgttcgac
      601 catcggcgtc gcctcgttgc cgctgaatgc gcaggtcgaa atcgacatga tcgcggtgct
      661 cgactgagcg cgcgccgtcg cgcatgcgcg agcttgtcgt tccatccgat gcggcactga
      721 cgacgttgcc cggcgtcggt ccggcgctgg cgaaaaccct ggaacgcctc ggcctgcgca
      781 cgctgcagga tttgtggttc cacctgccgc tgcgctacga agatcgcacg cgcctgacgc
      841 ctattcgcga tttggttccc ggtgctccgg cacaggtcga aggccgcgtc gaggccgtcg
      901 aacgcggctt ccgttatcgt ccgcaactgc gcgtggccgt cggcgacgat tcgcgtgcga
      961 cgctgctgtt gcgctttttc catttcaatt ccgcgcaggc gaaccagctc gtcgtcggca
     1021 cgcgtctgcg ctgctacggc gaggtgcgca gcggtgcgca gggtctggaa atggtgcatc
     1081 cgcagtaccg gcgagtggcc gatgccgaaa ccgctgtggt cgaggaatgc ctcacgccgg
     1141 tgtatccggt gacggaaggc ttgacttcca cgcggctcgg cggggtgatc gcgcgagcct
     1201 tgcaacgcct atcgaccgat gccgaactgg aactgctgcc gccggatttg cgcaagacgc
     1261 acaaattatc ctcgttgcgc gcggccttgc tttacgtgca ccgcccgccg ccgaacgcgg
     1321 acctcgcgca acttgccctc ggcgcgcatc cggcgcagca gcgcctcgct ttcgaagaac
     1381 tgctggccca gcacctgagc ctcaagcgct tgcgtgcgcg catccgcgag catgccgcgc
     1441 cggcgctgcg cggcgccggc gatttgcgca aacgcttcct cgccggcctg ccgttcgcgc
     1501 ccacgcacgc gcaggcgcgc gtcgcggccg aggttgcgcg cgacctcggc aaacccgcgc
     1561 cgatgctacg cctggtgcaa ggcgatgtcg gctcgggcaa gaccgtggtc gcggcgctgg
     1621 cggcgctcgc cgcagtcgag tcggattcgc aggctgcgct gatggcgccg acggaactgc
     1681 tggccgagca gcactaccgc aatttccaga gatggatgga gccgcttggc atcgaggtag
     1741 tctggctcgc cggcaagctc aagggccgcg tgcgcagcgc cgcgctggcg aagattgcct
     1801 cgtctgcgca gatcgtcatc ggcacgcatg cgctgatgca ggaaggcgtc gcgttccgcg
     1861 atctcgcact cgtcatcatc gacgagcagc accgcttcgg
//
LOCUS       DASRVO010000245         1961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_189646, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000245 DASRVO010000000
VERSION     DASRVO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189646"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..943
                     /locus_tag="VFF05_03020"
     CDS             17..943
                     /locus_tag="VFF05_03020"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZW50804.1"
                     /translation="MVNQQATAFAPASVGNIGVGFDILGHTIAGPGDRATVRRIPEAD
                     VHISAIRGSAAELPRDPEHNTAGAALIALRGALRLPFGFEIELDKGIPFCSGMGGSAA
                     SCVAALVAANALLDPQLPASDLYPFALTGEAVASGSAHGDNVGPMLLGGMVLATANRL
                     VPIAVPVSWHCAVAHPHVALETRRARAALKGDYALHEFVGQSANLALFLTGCQRGDPE
                     LVRAGLNDVLIEPRRAPLIAGFARVKQAALDHAALGASISGGGPSVFAWFESRAQAEA
                     AAPAMQAAFADAGLDSDVFVSPVSGPAAAIVP"
     gene            940..>1961
                     /gene="thrC"
                     /locus_tag="VFF05_03025"
     CDS             940..>1961
                     /gene="thrC"
                     /locus_tag="VFF05_03025"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZW50805.1"
                     /translation="MKFISTRGNSSATTLSAAIVAGLAPDGGLYVPAMLPHISPDDFS
                     GCNALAKVASRLLAPFFEGDALARELDAICAETFTFDAPLKALATPRDFILELFHGPT
                     AAFKDFGARFLAACMRRLPRESRKDLTILVATSGDTGAAVGAAFVGVPGFRVVILYPD
                     GRVSPRQAHQLGCFGGNVAALRVAGSFDDCQRLVKQAFADAKLRAQIPLSSANSISLG
                     RLLPQTAYFAHAALAHRHTQGTLLNFVVPTGNLGNAFACMLARACGLPIGRIALATNA
                     NLTLHDFFSGADYAPRASVATLANAMDVGAPSNFERLRWLFPDDEKLRREFCVDAIGD
                     EAIAAT"
BASE COUNT          292 a          685 c          629 g          355 t
ORIGIN      
        1 cgtcgctggg agctcgattg tgaatcagca agccaccgca ttcgcgccgg catcggtcgg
       61 caacatcggc gtcggtttcg acatcctcgg ccacaccatc gccgggcccg gcgaccgggc
      121 cacggtaaga cgcattcccg aagcggatgt gcacatcagc gccattcgcg gcagcgccgc
      181 ggaactgccg cgcgatcccg agcacaacac cgcgggcgcg gcgctgatcg ccctgcgcgg
      241 cgcgctgcgc ctgccgttcg gtttcgagat cgaactcgac aagggcattc cattctgctc
      301 cggcatgggc ggctcggcgg cgtcctgcgt cgccgccctc gtcgccgcga atgccttgct
      361 cgatccgcaa ctcccggcat ccgatttata tccgttcgca ctgacgggtg aagccgtcgc
      421 gagcggcagc gcccacggcg acaacgtcgg accgatgctg ctcggcggca tggtgctcgc
      481 gacagcgaat cggctggtgc cgatcgcggt tcccgtgagc tggcattgcg cggtcgcgca
      541 tccgcatgtc gcgctggaga cccggcgcgc acgcgcggcg ctcaagggcg actacgcgct
      601 gcatgaattc gtcggtcaaa gcgccaacct tgcgctgttc ctcaccggct gtcagcgcgg
      661 cgatccagaa ctcgtgcgcg cgggcctcaa cgacgtgttg atcgaaccgc gccgcgcgcc
      721 gctgatcgcg ggattcgctc gcgtcaagca ggccgcgctg gatcacgccg cgctcggcgc
      781 cagcatttcc ggcggcggcc ccagcgtgtt cgcctggttc gagtcgcgcg cgcaggccga
      841 agccgccgcg ccggcgatgc aagcggcgtt cgccgacgcc ggactcgaca gcgatgtttt
      901 cgtctcgccg gtgagcgggc cggcagcggc tatcgtgccg tgaaattcat cagcacgcgc
      961 ggcaattcct ctgccacgac gttgagcgca gccatcgtgg cgggactcgc gcccgacggt
     1021 ggcctctacg ttcccgcgat gctgccgcac atttcgcccg atgatttttc cggatgcaat
     1081 gcgttggcaa aggttgcgtc gcggctgctc gcgccgttct tcgaaggcga cgcgctcgca
     1141 cgcgaactcg acgcaatctg cgccgaaact ttcaccttcg atgcgccatt gaaggctctg
     1201 gcgacgccgc gcgatttcat cctcgaactc ttccatggcc cgaccgcggc gttcaaggat
     1261 ttcggcgcgc gcttcctcgc ggcttgcatg cgccggctgc cgcgtgaatc acgcaaggac
     1321 ttgaccatac tcgttgccac ctccggcgat accggtgcgg cggttggcgc cgcgttcgtc
     1381 ggcgtaccgg gttttcgcgt cgtgatcctg tacccggatg gccgcgtttc gccgcgccag
     1441 gcacatcaac tcggctgctt cggcggcaat gtcgcggcgt tgcgcgtggc cggatccttc
     1501 gacgattgtc aaaggctggt caagcaggct ttcgccgatg ccaaactacg cgcccagata
     1561 ccgctgtcgt cggccaacag catcagtctc ggccgcctgc tgccgcagac ggcctacttc
     1621 gcgcacgccg cgctggcgca caggcacacg cagggcacgc tgctgaactt cgtcgtgccg
     1681 accggcaatc tcggcaacgc ttttgcgtgc atgcttgcgc gcgcctgtgg cctgccgatc
     1741 ggccgcatcg cgctggcaac gaatgcgaat ctcacgctgc acgatttctt ttccggtgcc
     1801 gactatgcgc cgcgcgcgag cgttgccacg ctcgcgaacg ccatggacgt gggcgcgccg
     1861 agcaatttcg aacgcctgcg ttggctgttt cccgacgacg aaaagctgcg ccgcgaattc
     1921 tgcgtcgatg cgatcggtga cgaagcgatc gcggcgacga t
//
LOCUS       DASRVO010000246         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_189699, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000246 DASRVO010000000
VERSION     DASRVO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189699"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1495)
                     /locus_tag="VFF05_03030"
     CDS             complement(167..1495)
                     /locus_tag="VFF05_03030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HZW50806.1"
                     /translation="MSNVKTFHLPDLGEGLPDATIVEWLVKEGDQIKLDAPLVSMETA
                     KAVVEVPSPYTGKVTKLHGKAGDVIDTGAALAEFELDPNAKQRAENAAGGHHHAPAPA
                     AAAKPAPAPAAAKTADREDEGTVVGAMVSSNEVIVEQAASVGGVKAVPAVRAMAKKLK
                     VDLARVPPTGAGGAVTMQDVKNAAAAGTATASAAPAPVRAQSAPAPVAVRTALSASGQ
                     PMRTAPPSAQQVFGQPDQLKGVRRNMARVMADAHAQVVQTTIVDDADLHEWIGKQDIT
                     ARLIRAIVSACKAVPALNAWFDGKNLTRTLHPHVDIGIAVDTDDGLFVPALRNADVLD
                     AVGIRAAIKRLRTTVEDRSIPASELSGYTISLSNFGMFAGRYGTVVVVPPCVAIVGAG
                     KLSHDVVAVMGGIEVHRRLPISLTFDHRACTGGEAARFLKALLDDLALPR"
     gene            complement(1503..1664)
                     /locus_tag="VFF05_03035"
     CDS             complement(1503..1664)
                     /locus_tag="VFF05_03035"
                     /inference="COORDINATES: protein motif:HMM:NF018672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HZW50807.1"
                     /translation="MVWGLLAHDFHVKIFFLACVILAGLLGAMTVHRKVFFVQALPAI
                     IALVLLHLP"
BASE COUNT          263 a          540 c          578 g          297 t
ORIGIN      
        1 aattcgatgt tgcaacccgc gacgatgcgt ttgccgaagc gcgcggcggc gccgaccttg
       61 aatcgcgacc acggcgcgtg cgcgtgctcg cgcgccgcca gtgccgcgtc gatcagtctg
      121 cggtcgcccg ctgtgagcga cgtggcgggt gcttccttga ccacggtcag cgcggtagcg
      181 cgaggtcgtc cagcagcgcc ttgaggaagc gcgccgcttc gccgccagtg caggcgcgat
      241 gatcgaaggt gagcgagatc ggcaggcgcc ggtgcacctc gatgccgccc atcaccgcga
      301 ccacgtcgtg cgagagcttg cccgcgccga cgatggccac gcacggcggc accaccacca
      361 cggtgccgta gcgtccggcg aacatgccga agttcgagag gctgatcgtg tagccggaca
      421 gttccgaagc cggaatcgag cgatcttcga ccgtcgtgcg caggcgtttg atcgcggcgc
      481 ggatgccgac cgcgtcgagc acgtcggcat tgcgcagcgc cggcacgaac aggccgtcgt
      541 ccgtatccac ggcgatgccg atgtccacgt gcggatgcag ggtgcgggtg agattcttgc
      601 cgtcgaacca ggcgttgagc gcgggcacgg ccttgcacgc cgatacgatc gcgcggatca
      661 ggcgcgcggt gatgtcttgc ttgccgatcc attcgtgcag gtcggcatcg tccacgatcg
      721 tggtctgcac gacttgcgcg tgcgcgtcgg ccatgacccg cgccatgttg cggcgcacgc
      781 ccttgagctg atcgggctgc ccaaacactt gttgcgccga tggcggtgcg gtgcgcatgg
      841 gctggccgga ggcggacagc gcagtacgta cggcgaccgg cgcaggcgca gattgtgcgc
      901 gcaccggcgc gggtgctgcg cttgccgttg cggtacccgc cgcggcggca ttcttcacat
      961 cctgcatggt gaccgcgccg ccggcgccgg tcggcggcac gcgcgcgaga tcgactttca
     1021 gtttcttcgc catcgcgcgc acggctggca cggctttcac gccgccgacg ctggcggctt
     1081 gttcgacgat gacttcgttg ctcgaaacca tcgcaccgac cacggtgcct tcgtcttcgc
     1141 ggtccgcggt cttggcagcc gccggtgctg gagccggctt ggctgccgcc gctggcgcag
     1201 gtgcatgatg atgtccgccc gctgcgttct cggcgcgctg cttggcgttc gggtcgagtt
     1261 cgaattcggc caacgccgcg ccggtgtcga tcacgtcgcc ggccttgccg tggagtttcg
     1321 tcaccttgcc ggtatagggc gagggtacct cgaccacggc cttggcggtt tccatcgaca
     1381 ccagcggcgc atcgagcttg atttgatcgc cttccttgac cagccattcg acgatggtgg
     1441 cgtcgggcag gccttcgccg agatcgggca gatgaaaagt cttgacgttc gacatggcga
     1501 cctcagggta gatgcagcaa taccagcgcg atgatggccg gcagtgcttg tacgaagaaa
     1561 accttgcgat gtacggtcat ggcgccgagc aggccggcca ggatcacgca ggcaagaaag
     1621 aagattttca cgtggaaatc gtgagcgagc agaccccaaa ccagtccggc aacgagaa
//
LOCUS       DASRVO010000247         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_240945, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000247 DASRVO010000000
VERSION     DASRVO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240945"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFF05_03040"
     CDS             <1..774
                     /locus_tag="VFF05_03040"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZW50808.1"
                     /translation="VFVMDAFGTAHRAQASTHGVIRRAHVACGGPLLMAELDALAKAL
                     ERPARPLLAIVAGSKVSTKLALLENLLARVDQLIVGGGIANTFIAAAGHNVGKSLVER
                     DLLEVARRIGAEAKARGAEVPVPTDVVVAPEFAASAPATVKKVEAVAPGDMILDIGPD
                     TAVRYAALIAKSGTVVWNGPVGVFEFDAFGKGTQTLAQAIAKSNAFSIAGGGDTIAAV
                     EKYGVEANISYISTGGGAFLEFLEGKELPAVAALKARSA"
     gene            complement(771..>1203)
                     /locus_tag="VFF05_03045"
     CDS             complement(771..>1203)
                     /locus_tag="VFF05_03045"
                     /inference="COORDINATES: protein motif:HMM:NF022338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZW50809.1"
                     /translation="HSPIHAATPEQADLIGIVVVCALVMLLLALLLLMQCARVHVDQD
                     TLIVHTGVGTKRIPLANLHTHGLRLVNFSTHPELRPVVKLWGTGLPGFAGGWFKLRNG
                     DKAVCLLLDRHGISYLRSDADKLTVLLSLAEPEKLRALLGR"
BASE COUNT          206 a          396 c          412 g          189 t
ORIGIN      
        1 gtgttcgtca tggatgcctt cggcaccgcg catcgggcgc aggcatcgac ccacggcgtg
       61 atccggcgcg cgcatgtcgc ctgcggcggc ccgctgctga tggccgaact cgacgcgctg
      121 gccaaggcgc tcgaacggcc ggcgcgaccg ctgcttgcga tcgtggcggg ctccaaggtc
      181 tcgaccaagc tcgctctgct ggaaaacctg ctcgcgcgcg tcgaccagct catcgtcggc
      241 ggcggcatcg ccaatacgtt cattgccgcg gccggacaca acgtcggcaa gtcgctggtc
      301 gagcgcgacc tgctcgaggt cgcgcgccgc attggcgccg aggccaaggc gcgcggcgcc
      361 gaagtgccgg tgccgaccga tgtcgtcgtc gcgccggaat tcgcggcgag cgcgccagcc
      421 acggtgaaga aagtggaagc tgtcgcaccc ggcgacatga tcctcgacat cggcccggac
      481 accgcggtgc gctatgccgc gctgatcgcc aagtccggca cggtggtctg gaacggaccc
      541 gtcggcgtgt tcgagttcga cgcgttcggc aagggcacgc aaacgctcgc gcaggcgatc
      601 gcgaagtcga acgcgttttc catcgccggg ggcggcgaca ccatcgcggc cgtggaaaaa
      661 tacggcgtgg aggcaaatat ttcctacatc tccaccggcg gcggcgcgtt cctggaattc
      721 ctcgaaggca aggaactgcc ggcggtggcg gcgttgaagg cccgttccgc ctagcgtccc
      781 agcagcgcgc gcagtttctc cggctcggcg agcgacaaca gcacggtaag cttgtccgcg
      841 tcgctgcgca ggtacgaaat cccgtggcgg tcgagcagca ggcacacggc cttgtcgccg
      901 ttgcgcaact tgaaccagcc gccggcgaac cccggcagcc cagtgcccca gagcttgacg
      961 acgggccgca gttccggatg cgtgctgaaa ttcacgaggc gcaatccgtg cgtgtgcagg
     1021 ttcgcgagcg gtatgcgctt ggtgccgaca ccggtatgca cgatcagggt gtcttgatcc
     1081 acgtgcacac gcgcgcattg catcagcagc agtagcgcaa gtagcagcat gacaagcgcg
     1141 cacacgacga cgatgccgat caggtcggcc tgttccggcg tcgcggcatg tatgggcgaa
     1201 tgg
//
LOCUS       DASRVO010000248         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_59395, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000248 DASRVO010000000
VERSION     DASRVO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59395"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /gene="crp"
                     /locus_tag="VFF05_03050"
     CDS             <1..525
                     /gene="crp"
                     /locus_tag="VFF05_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HZW50810.1"
                     /translation="FIIEGSVSVLTEDEEGRELILAYINAGEFIGEMGLFVETPRREV
                     MVRTRTSCDLAEISYERLFQLCEGPLREDCAKVLFAIGTQLTNRLLHTSRKVSRLAFM
                     DVTSRVAKTLIDLTEEPDAMSHPKGTQIRISRQEISRIVGCSREMVGRVLKQLEQQGM
                     ISVAGKTIVVFGTR"
     gene            complement(522..1310)
                     /gene="trpC"
                     /locus_tag="VFF05_03055"
     CDS             complement(522..1310)
                     /gene="trpC"
                     /locus_tag="VFF05_03055"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZW50811.1"
                     /translation="MSDILRRILARKAEEVRERRERVALGTLRAQALAAPATRGFAAA
                     LRANIAAGKPAVIAEVKKASPSQGVIRADFRPTDIARSYAAGGAACLSVLTDADFFQG
                     ADAYLQQAHAACALPVLRKDFVIDAYQILEARVLGADCVLLIVAALDDAQLTEFSALA
                     RDLGMDVLVEAHDAVELERALKTSAALIGINNRDLRTFATSLDTTLGLRSRVPADRLL
                     VTESGIHTSADVARMRAAGVHAFLVGEAFMRAENPGAELARLFS"
     gene            complement(1307..2338)
                     /gene="trpD"
                     /locus_tag="VFF05_03060"
     CDS             complement(1307..2338)
                     /gene="trpD"
                     /locus_tag="VFF05_03060"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZW50812.1"
                     /translation="MITPQEALQRTIEHREIFHDEMVDLMRQIMRGEVSPAMVAAILS
                     GLRVKKETIGEIAAAASVMREMAVRVHVPPYRHFVDIVGTGGDGANSFNISTAAMFVT
                     AAAGARVAKHGNRSVSSKSGSADVLEALGARIDLGPEQVAVCVEETGIGFMFAPNHHP
                     AMRVVAPVRREMGVRTLFNILGPLTNPAGAPNMLMGVFHADLVGIQVRVLEHLGAAHA
                     LVVWGRDGIDEISLSDTTLVGELRDGKVREYEIAPEHFGFARAAREDFAVANATESRA
                     RVLTALGNEPGPVRDIVTLNAGAALYAADVVDSIADGVERAREAIASGTARAKLDQFL
                     AATRRLAGA"
     gene            complement(2341..2937)
                     /locus_tag="VFF05_03065"
     CDS             complement(2341..2937)
                     /locus_tag="VFF05_03065"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZW50813.1"
                     /translation="MLLMIDNYDSFTFNLVQYLGELGEDVRVVRNDALTVEDIRELAP
                     ESIVISPGPGTPDEAGVTLETIAKLGGTIPILGVCLGHQSIGQAFGGRVIRAPQVMHG
                     KTSPIHHNGKGVFAGLRNPFAATRYHSLVVEKSSLPTSLEVTAWTQNADGSIDEIMGL
                     RHKTLAIEGVQFHPESILTEHGRDLLRNFLRSPLPKAA"
     gene            complement(3002..>3848)
                     /locus_tag="VFF05_03070"
     CDS             complement(3002..>3848)
                     /locus_tag="VFF05_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50814.1"
                     /translation="QAGIHGTGAPGGSDGFDGSALGNSIFLRSGASLTLLAQDAADLL
                     TLGPQVDFTDDTVFGAGGTNVFVKGNGTVVYNGTADYQGTVKIDNANFKVNGQIDQAP
                     VFVCRNSSFSSQRGILSGIGILSGAVFANSGTISPDAGGTLTLGGLSLNAADPINNTL
                     GSLVHIDISSGGTSLVAVTGAASLAGTLEINLDPAANHAGSYIVLTSSGITGTFDSVT
                     FTGATPASYSVSYLPTGAPTYVQIDLTGAAAAATPLPVNPWWALAALALVLLGGATLA
                     NRRRL"
BASE COUNT          696 a         1229 c         1241 g          682 t
ORIGIN      
        1 ttcatcatcg aaggttcggt cagcgtgctc accgaggatg aggaaggccg cgaactgatc
       61 ctcgcctaca tcaacgccgg agaattcatc ggcgagatgg gcttgttcgt ggaaaccccg
      121 cggcgcgagg tgatggtgcg cacgcgcact tcctgcgatc tggccgagat cagctatgag
      181 cgcctgttcc agttgtgcga aggcccgctg cgcgaggatt gcgccaaggt gctgttcgcg
      241 atcggcacgc aactgaccaa ccgcctgctg cacacctcac gcaaggtcag ccgcctcgct
      301 ttcatggacg tgaccagtcg cgtggccaag acgctgatcg acctcaccga ggaacccgac
      361 gcgatgtcgc accccaaggg cacgcagatc cgcatatcgc gccaggagat cagccgcatc
      421 gtcggctgct cgcgcgagat ggtcggacgc gtgctcaagc agctcgagca gcaagggatg
      481 atcagcgtcg cgggcaagac gatcgtggtg ttcggaactc gttaggaaaa caaacgcgcc
      541 agttccgcgc ccggattttc cgcgcgcatg aacgcttcgc cgacgaggaa cgcatgcacg
      601 ccagccgcgc gcatccgcgc cacgtccgca gacgtgtgga tgccgctttc ggttaccagc
      661 aatcgatctg ccgggacgcg cgaacgcaag cccagtgtcg tatcgagcga agtcgcgaac
      721 gtgcgcagat cgcggttgtt gatgccgatc agcgccgcgg aagtcttcaa tgcgcgttcg
      781 agttcgacgg catcgtgcgc ttcgaccaac acgtccatcc cgaggtctcg cgccagtgcc
      841 gaaaattccg tgagctgcgc gtcgtccagc gccgccacga tcagcaggac gcaatccgca
      901 ccgagaacgc gtgcctcgag gatctggtat gcgtcgatga cgaagtcctt gcgcagcaca
      961 ggcagcgcac atgctgcgtg cgcctgttgc aggtacgcat cggcaccctg gaagaaatcc
     1021 gcgtcggtga gcacggacaa gcaggcagcg ccgccggcag cgtaactgcg cgcgatgtcg
     1081 gttgggcgaa aatccgcgcg gatgacgccc tggctcgggc tggccttttt cacctcggcg
     1141 atcacggcgg gctttcctgc cgcgatattc gcgcgcagcg ccgcagcgaa accgcgcgtg
     1201 gccggcgcgg cgagtgcctg cgcgcgcagc gtgccgagag caacgcgctc gcgacgttcg
     1261 cgcacctcct cggccttgcg cgcgaggatg cgtcgcaaga tgtcgctcat gcgccagcca
     1321 gtcgccgcgt cgcggcgagg aattggtcga gcttggcgcg tgccgtgcca ctggcaatgg
     1381 cttcgcgcgc tctttcgaca ccatccgcga tggagtcgac cacatcggca gcgtacagcg
     1441 ccgccccggc gttgagcgtc acgatgtcgc gcaccgggcc gggttcgttg cccagcgcgg
     1501 tgagcacgcg cgccctggat tcggttgcgt tggcgaccgc gaagtcctcg cgcgcggcgc
     1561 gcgcgaagcc gaagtgctcc ggcgcgattt cgtattcgcg caccttgccg tcgcgcagtt
     1621 caccgacgag cgtcgtgtcg ctgagcgaaa tctcgtcgat accgtcgcgg ccccagacca
     1681 ccagggcatg agcggcgcca aggtgttcga gcacacgcac ctggatgccg accaggtccg
     1741 cgtggaacac gcccatcagc atgttcggcg cgccggccgg attggtcagc gggccgagga
     1801 tgttgaacag ggtgcgtacg cccatttcgc gacgcaccgg cgcgaccacg cgcatcgcag
     1861 gatggtggtt cggcgcgaac atgaaaccga taccggtttc ctccacgcat accgccacct
     1921 gttcgggacc cagatcgatg cgcgcgccga gcgcttcgag cacgtcggcg ctgccggatt
     1981 tggacgacac gctgcgattg ccgtgcttgg ccacgcgcgc acccgccgct gcggtgacga
     2041 acatcgcggc ggtggaaatg ttgaacgaat tggcgccgtc gccgccggtg ccgacgatgt
     2101 ccacgaaatg ccggtatggc ggcacgtgca cgcgcactgc catctcgcgc atcacgctcg
     2161 ccgcggcggc gatctcgcca atggtttcct tcttcacgcg cagaccggac aaaatcgccg
     2221 cgaccatcgc cggcgagact tcgccgcgca tgatctggcg catcaggtcg accatctcgt
     2281 cgtggaaaat ctcgcggtgt tcgatcgtgc gctgcaacgc ttcctgcgga gtgatcatgc
     2341 tcaggccgcc ttcggcaacg gcgaacgcag aaaattgcgc agcaggtcgc ggccatgttc
     2401 ggtcaggatg gattccggat ggaactgcac gccttcgatg gccagcgtct tgtggcgcag
     2461 gcccatgatc tcgtcgatcg aaccatccgc gttctgggtc catgccgtga cttccaggct
     2521 cgttggcaac gaggattttt ccaccaccag cgaatggtaa cgcgtggccg cgaacggatt
     2581 gcgcaggccc gcgaatacgc ccttgccgtt gtggtgaatc ggcgacgtct tgccgtgcat
     2641 gacctgcggc gcgcggatca cgcgtccgcc gaaggcctgt ccgatgctct gatggccgag
     2701 gcacacgccg agaatcggaa tcgtgccgcc gagtttcgcg atcgtctcca gcgtcacgcc
     2761 ggcttcgtcc ggcgtaccgg ggccgggcga aatcacgatg ctctccggcg ccagttcgcg
     2821 gatgtcctcg accgtcagcg catcgttgcg caccacgcgc acatcctcgc ccaactcgcc
     2881 caaatactgg acgaggttga aagtgaagga gtcgtaattg tcgatcatca gcagcatata
     2941 gcctcgatcc ggagatttgc gaaaagggtt ctggctggac cttgctccgc cgcggagcgc
     3001 atcagagccg tcggcgattc gccagcgtag cgccgcccag caagacgagc gccagggcag
     3061 ccagagccca ccagggattc accggcagtg gcgtggcagc ggcggcggca cctgtcagat
     3121 cgatttgtac ataggtcgga gctccggtcg gcaggtagga aaccgaataa cttgccggcg
     3181 ttgcccccgt aaacgtgacg ctgtcgaaag tgccggtaat gccagacgac gtgagaacaa
     3241 tgtacgagcc ggcgtgattg gcggccgggt cgagattgat ttccagggtg ccggccagtg
     3301 acgcggcccc ggtaacggca accagggatg tgccgccgga actgatgtcg atatggacaa
     3361 ggctgcccag ggtattgttg atggggtcgg ccgcattgag gctcaatccg ccgagcgtga
     3421 gcgttcctcc ggcgtcggga gagatcgttc cggaatttgc gaaaaccgct ccgctcagga
     3481 tcccgattcc gctgagtatt cctcgttgcg aactgaagct ggagtttctg cacacgaaaa
     3541 cgggcgcctg gtcgatctga ccgttcactt tgaagttggc attgtcgatt ttgacggtgc
     3601 cttgatagtc ggcggtcccg ttgtaaacga cggttccgtt tcccttgacg aagacgttgg
     3661 ttccacccgc gccgaaaacg gtatcgtcgg taaagtcgac ctgcgggcca agggtcagaa
     3721 gatcggcggc atcctgcgcc agcagcgtga gcgacgcccc gctgcgcagg aaaatgctgt
     3781 ttcccaatgc cgatccgtcg aagccgtccg agccgccggg tgcaccggtt ccatggattc
     3841 ccgcttgc
//
LOCUS       DASRVO010000249         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_344895, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000249 DASRVO010000000
VERSION     DASRVO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344895"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..599)
                     /locus_tag="VFF05_03075"
     CDS             complement(63..599)
                     /locus_tag="VFF05_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HZW50815.1"
                     /translation="MHVWTVGHSTRTAEEFIAVLGAYGVETVADVRRFPGSRRYPHFA
                     AEALAALLRSRALEYVWLRKLGGRRRPTGSPTASAWRNPSFQAYADYMLSEEFAEGLH
                     ELLHVAAGSRTAVMCSELLWWRCHRSLIADALLFNGTQVTHILTASKSAEHPYTSPAR
                     IVDGQLTYAAGEDQASGI"
     gene            complement(696..884)
                     /locus_tag="VFF05_03080"
     CDS             complement(696..884)
                     /locus_tag="VFF05_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50816.1"
                     /translation="MLSILPTAVYPFLFAVIAAIAASVFGRQSTSKPFALVSAVLYAL
                     AVACVIIGVITIFLDRAT"
BASE COUNT          201 a          394 c          402 g          193 t
ORIGIN      
        1 catcatctcc gaaccagcgg ccgcgcgcgt ctgaccggcg ctggtgcttg ccgcggcagg
       61 cgtcatatac cggacgcctg atcttcgccg gccgcgtacg tcaactgccc gtcgacgatg
      121 cgcgccggcg acgtgtaggg atgctcggcg ctcttgctcg ccgtgagaat gtgcgtgacc
      181 tgcgtgccgt tgaacagcag cgcgtcggcg atcagcgagc ggtgacagcg ccaccacagc
      241 agctcggagc acatcacggc cgtcctggag ccagcggcga cgtgcaacag ctcgtgcagg
      301 ccctcggcaa attcctcgga cagcatgtaa tcggcatacg cctggaacga cggattgcgc
      361 catgccgagg ccgtcggcga acctgtcggg cggcgccggc caccgagctt gcgcagccac
      421 acgtattcca gtgcgcgcga ccgaaggagc gccgccagcg cctcggcagc gaagtgcgga
      481 tagcgccgcg aacccggaaa acgccgcaca tccgcgaccg tttcgacgcc gtaggcaccg
      541 agcacggcga tgaactcctc tgcggtgcgg gtcgaatggc cgacagtcca gacgtgcatg
      601 atcgccagcg cgtgtcgagc ttggttcgtc gccgatccgg ccgcctctcg cgcttgcgcc
      661 gcaggggcga cgtgacgccg cgcggctgcg gccggctaag tggcccggtc gagaaagatc
      721 gtgatcacgc cgatgatgac gcatgccacc gcgagcgcgt acaggacagc gctgaccagc
      781 gcaaacggct tgctggtgga ctgtcggccg aagacgcttg ccgcaatggc cgcgatgacg
      841 gcaaacagaa atggatagac cgcggtcgga agaatgctca gcacgacgac ctcgcttgtt
      901 ccgtatctga tgcaactgtg cgtcagccgg atcgcgcggc gagtgggccg gttccgacga
      961 agcgcgtagg cccggaccgc tacgattcgg gcctcctgag cgttccgcgt tgagttgact
     1021 ggagcgccgc acccaaggag cgccgaacgc tcaataccga ccgtgaaacg ctgttacttc
     1081 gcggccgaag cggcgcttgc cgccggcgaa gccttcctgg cgctggcgtg cttgcgcgcc
     1141 ttcttgtgcg agcgcttgtg ggccttggcc ttggccttgt gcttggcttt
//
LOCUS       DASRVO010000250         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_163096, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000250 DASRVO010000000
VERSION     DASRVO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163096"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /gene="era"
                     /locus_tag="VFF05_03085"
     CDS             <1..653
                     /gene="era"
                     /locus_tag="VFF05_03085"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZW50817.1"
                     /translation="ARQAIAEADVAVQIVEAGRWTVEDTQVWTALDARRPRLIAINKI
                     DAQKNKAALLPFIERIGVERAPDAVYLVSARRRDGLDDLVRGIVAHLPVGAATYAEDE
                     VTDRSERFLAGEMVREQAMLRLANELPYAATVEVTEFSEENERVVIHATIWVEREGQK
                     AIVIGAGGAQLKAIGTAARRVMEKAFGRRVHLQLWVRVRENWSDDEAALKRFGYAE"
     gene            653..1384
                     /gene="recO"
                     /locus_tag="VFF05_03090"
     CDS             653..1384
                     /gene="recO"
                     /locus_tag="VFF05_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZW50818.1"
                     /translation="MRVEQQPAFVLHARAWRETSLLLDVLSRDHGRIGLVARGVRGTR
                     VRTPRSLLQPFAPLSVSWSGRGELATMHAAEAAGAALPLAGEVLLCALYLNELVVRLA
                     PRSDPHPDMFATYLKTLARLAHGDAPAWTLRRFERDLLSQLGYGLQLDVEADTGESIE
                     AAVEYAYFLEHGPVRWRGAGDGLKLRGAALLALARDEEPDVSDLPSLRRLLRALIAQR
                     LDGGALNAWNLMVGTRSAGANLAEL"
     gene            complement(1365..>1953)
                     /locus_tag="VFF05_03095"
     CDS             complement(1365..>1953)
                     /locus_tag="VFF05_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW50819.1"
                     /translation="ADGAAALAADNAFDLLLIDRHLPDIGGIELLHALRKNGITATAI
                     ATSAEIDADAAAQLHAAGFVDVLEKPASVQRIVEIIVRHLPMATPILLDDVAALDAIG
                     GDADALRALRDLLARELEQLEHDLTHADLATNPSRLGERLHRLRASCGFCGASALATC
                     AGEWQAALRAGSPSANALRDAFVAVCRETVIALRG"
BASE COUNT          323 a          626 c          676 g          328 t
ORIGIN      
        1 ccgcgcgcca ggccatcgcc gaggccgatg ttgcggtgca gatcgtcgag gccgggcgct
       61 ggacggtcga agacacgcag gtgtggaccg cgctggatgc gagacgaccg cgcctgatcg
      121 ccatcaacaa gatcgatgcg cagaagaaca aggccgcgct gctgccgttc atcgaacgca
      181 ttggcgtcga acgcgcgccg gatgcggtgt acctggtgtc ggcacgccgc cgcgacggcc
      241 tggacgatct cgtgcgcggc atcgtcgcgc acttgccggt cggcgccgcg acctacgccg
      301 aggatgaggt cacggatcgc agcgagcgtt ttctggccgg cgagatggtg cgcgaacagg
      361 cgatgctgcg cctggcgaac gaattgccct acgccgcgac ggtggaagtg acggaattca
      421 gcgaagagaa tgaacgcgtc gtcatccatg cgacgatctg ggtcgagcgc gaagggcaga
      481 aggccatcgt catcggcgcc ggcggcgcac agttgaaggc gatcgggact gcggcgcgcc
      541 gggtcatgga aaaggctttt ggccgccgcg tgcatctgca actgtgggtg cgcgtgcgcg
      601 agaactggtc ggatgatgag gcggcgctga agcggtttgg ctacgccgag tgatgcgcgt
      661 cgaacaacaa cccgccttcg tgctgcacgc gcgcgcgtgg cgcgaaacct cgctgctgct
      721 cgatgtgctc agccgcgacc acggccgtat cggactcgtc gcgcgcggcg tgcgcggcac
      781 gcgggtgcgc acgccacggt cgctgctgca accgttcgcg ccgttgtccg tgagttggag
      841 cgggcgtggc gagctggcga cgatgcatgc ggccgaagcg gccggcgccg cactgccctt
      901 ggctggtgaa gtcctgttgt gcgccttgta cctcaacgag ctcgtcgtgc gcctggcccc
      961 gcgcagcgat ccgcatccgg acatgttcgc gacatacctc aaaaccttgg cgcggctcgc
     1021 acacggcgat gcgccggcgt ggacgctgcg tcgcttcgag cgcgacctgc tcagtcaact
     1081 cggttacgga ttgcagctcg acgtcgaagc cgataccggc gaatccatcg aagccgccgt
     1141 cgaatatgcg tatttcctcg aacatggccc ggtgcgctgg cgcggcgcgg gcgacggctt
     1201 gaaattgcgc ggcgcggcct tgcttgcgct ggcgcgcgat gaagaaccgg acgtttcgga
     1261 tctgcccagc ctgcgccgcc tgctgcgcgc gctgatcgcc cagcgtctgg atggcggtgc
     1321 cctcaatgcg tggaatttga tggttggcac tcgtagcgct ggcgctaacc tcgcagagct
     1381 ataacggttt cgcggcagac ggcgacgaat gcatcgcgca gcgcgtttgc cgatggactc
     1441 ccggcgcgca gcgcagcctg ccactccccg gcacacgtcg ccagtgcact cgcgccgcag
     1501 aatccgcagg acgcgcgcag ccgatgcaaa cgctcgccca agcgcgaagg gttcgtcgcc
     1561 agatcggcgt gggtcaggtc gtgttcgagt tgttccagtt cccgggcgag caggtcgcgc
     1621 aaggcacgca gcgcatccgc atcgccgccg atggcgtcaa gcgcagcaac gtcatcgagc
     1681 aggatgggcg tggccatcgg caaatgccgc acgatgatct cgacgatgcg ttgaaccgat
     1741 gccggttttt ccagcacatc gacgaaccct gctgcatgca gttgcgccgc cgcatccgca
     1801 tcgatttccg cactggtagc gatcgccgtc gcggtgatgc cgttcttgcg cagcgcgtgc
     1861 agcaattcga tgccgccgat gtcgggcaag tggcggtcta tcagcagcag atcgaatgca
     1921 ttgtccgcag cgagcgccgc cgcaccgtcg gct
//
LOCUS       DASRVO010000251         1145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_344935, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000251 DASRVO010000000
VERSION     DASRVO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344935"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VFF05_03100"
     CDS             complement(<1..1021)
                     /locus_tag="VFF05_03100"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HZW50820.1"
                     /translation="MSTALVLLRRDLRLEDNPALHAASAEHERVLPVYIHAPDEEAPW
                     QPGAASRWWLHRSLQALDDSLARQKGRLCIARGETLGVMRALIREFDAATVYWNRRYE
                     PAAIASDTKLKAGLRTDGVAVESRSGNLWCEPWQIATEQGDPYRVFTPFWRKLRTRLQ
                     PDEPLPVACAREWVQAHGSLPLSALELLPKIAWDAGFDATWRPGEAGAAEMLGIFADD
                     ALNDYKTVRDLPARHGTSRLSPHLHFGEITPRQIHHRLHACASGDRSRRGPDFEPYLR
                     ELAWREFAHHLLYHFPNTPDENFNPRFDRFRWAMDNPDAIRSWQRGQTGIPLVDAGMR
                     ELWTTG"
BASE COUNT          188 a          367 c          386 g          204 t
ORIGIN      
        1 agccggtcgt ccacagctcg cgcatcccgg catcgacgag cgggattccc gtttgccccc
       61 gctgccagct tcggattgcg tctggattgt ccatggccca gcgaaatcgg tcgaagcgcg
      121 ggttgaagtt ttcgtccggc gtgttcggaa agtggtacag caaatggtgc gcgaactcgc
      181 gccacgcgag ttcgcgcagg tagggctcga aatcgggtcc gcgccggctg cggtcgccgc
      241 tggcgcatgc atgcagccgg tggtggatct ggcgcggcgt gatttccccg aaatgcaggt
      301 gcggcgacaa ccttgatgta ccgtggcgcg cgggcaggtc gcgcaccgtc ttgtagtcgt
      361 tgagtgcgtc gtccgcgaag atgcccagca tttcggccgc accggcttca ccgggccgcc
      421 acgtcgcgtc gaaacccgcg tcccaggcga tcttcggcaa cagttccagc gcggacaacg
      481 gcagactgcc gtgcgcttgc acccattccc gggcgcacgc cacgggcagc ggctcgtccg
      541 gttgcagtcg cgtgcgcagc ttgcgccaga acggcgtgaa gacgcggtaa gggtcgccct
      601 gctcggtggc gatttgccac ggctcgcacc acagattgcc cgatcggctc tcgaccgcga
      661 cgccgtctgt gcgcaagcca gccttgagct tcgtgtcgga ggcgatggcg gcgggttcgt
      721 agcgccgatt ccagtacacg gtcgcggcat cgaattcccg gatcaatgcc cgcatcacgc
      781 ccaaggtttc gccgcgagcg atgcaaagcc gtcctttctg tcttgcgagc gaatcgtcga
      841 gtgcttgcag cgagcggtgc agccaccatc ggctggcggc gccgggttgc cagggcgcct
      901 cctcgtcggg cgcatggatg tatacgggca gaacgcgctc gtgctccgcg gaggccgcat
      961 gcagggccgg gttgtcttcc aggcgaagat cccggcgcaa cagtaccagt gccgtgctca
     1021 tcggtggccc ggatcgaact ggtcgccaag cagaaggcga agggtcatca tccgaagatc
     1081 atgcgcttga tcgaaccgga tcgtgacgca ggcttcggta acgccaactg cacagcagcg
     1141 aagtc
//
LOCUS       DASRVO010000252         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_344943, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000252 DASRVO010000000
VERSION     DASRVO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344943"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFF05_03105"
     CDS             <1..643
                     /locus_tag="VFF05_03105"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZW50821.1"
                     /translation="TPGSVADQLGLRPGDVLVDINGTSLANLGADADGRALAAATFNS
                     SLADLAPTAPLRLRVLRDGVFLAMNAPLHAAATSASVAPAEGAGDPPVGGGCGRISTF
                     DVAPRSEHLYGAKILLLDGVTPGPTGTPSFRVSAGTHKLLVAENIPTREMGVGSYASL
                     RSRRDNSKELTVTVKPNTTALLAARLNTEKAADFAHGAYWDPVIWREIPEDCR"
     gene            complement(738..>1051)
                     /locus_tag="VFF05_03110"
     CDS             complement(738..>1051)
                     /locus_tag="VFF05_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50822.1"
                     /translation="RLREQQAGENLARGADLERGIGVGLLPAAEEPGLLLIRTSLSAI
                     DPADREPAVQTAVHALLQVRQIRIGRHRLRAKGGRYQRQQQEKSDLDRIIATPLSSRQ
                     A"
BASE COUNT          180 a          367 c          329 g          175 t
ORIGIN      
        1 cacgcccggc agcgtcgccg accaactcgg cttgcgcccg ggcgacgtgc tcgtcgacat
       61 caacggcacc tcgctggcga acctgggtgc cgatgccgac ggccgcgcac tcgccgcggc
      121 gacgttcaat tcctccctcg ccgatctcgc gccgacggca cccctgcgcc tgcgcgtgct
      181 gcgcgatggc gtgttcctgg ccatgaatgc gccattgcac gccgctgcca cgtcggcttc
      241 cgttgcacct gccgaaggcg cgggcgatcc gccggtcggc ggcggctgcg gccgcatcag
      301 cacgttcgac gtcgcgccgc gcagcgaaca cctttatggg gccaagatcc tgcttctcga
      361 cggcgtcacg cccggaccga ccggcacgcc atcgttccga gtttcggcgg gaacgcacaa
      421 actgctggtt gccgaaaaca ttccaacccg ggaaatgggt gttggctcgt acgcctcgct
      481 gcgctcgcgc cgcgacaaca gcaaggaact cacggtgacg gtgaagccga acacaaccgc
      541 actgctggcc gcgcggctga acaccgaaaa ggccgcggat ttcgcccatg gcgcatattg
      601 ggatccggtg atctggcgcg agatcccgga agattgccgc tgagcgcaga acctcttcca
      661 cgttgccgag tcgaacttcg cacgcgtccc acacgacgtc gccacaaaga tgtggccatt
      721 tcgtgcgtcc gcatcggcta tgcttggcgc gatgacaacg gcgtggcgat gatccgatca
      781 agatccgact tttcctgctg ctgacgctga taccgaccgc ccttggcgcg cagccggtgg
      841 cggccaatgc gtatctggcg aacctgcaac aaggcgtgga cagcggtctg taccgccggc
      901 tcgcgatcgg ctggatcgat ggcactcaaa ctggtccgga tcagcagcag acctggttcc
      961 tcggcagcgg gcaaaagccc gacgccgatt ccacgttcga gatcggcgcc gcgagcgaga
     1021 ttttcaccgg cctgctgctc gcgcaggcgg c
//
LOCUS       DASRVO010000253         1585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_137236, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000253 DASRVO010000000
VERSION     DASRVO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137236"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="VFF05_03115"
     CDS             complement(<1..1459)
                     /locus_tag="VFF05_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZW50823.1"
                     /translation="MRSLWNDADAARYSGPLGPRVYTSRLLGTDRSLVLHGGGNTSVK
                     LRQTNLFGASEDVLYVKGSGWDLETIEAAGFTPVALDYVRRLAQLPALSDPQMVNELN
                     THMLRAGAPSPSVETILHAILPHDYVDHTHADAVLSISNAPRGEQRVREIYGDKVVVI
                     PYIMAGFDLAAHCAREFAAQSGPQTIGMVLLSHGVFSFGADAKESYERMIGLVSMAED
                     YLAANKAWSVSLPSPRSSSISREAIAGLRRAISDLAEAPLILRVNDSPKFLSFAQHSA
                     LSRLSQRGPATPDHVIRTKPLPMLGFDVQAYARGYRDYFERQAAQAKEKKTMLDPAPR
                     MVLDPTLGFAAAGRTARDAAIIEELYDHTIDVILRAEALGGWQAVGERDIFDIEYWDL
                     EQAKLRKAGAPAPFAGEVVLVTGAASGIGKACVEAFLKRGAAVVGLDRNAAIETMWMR
                     PDFAGIACDVTDAKAVDAALGAAVKRYGGIDMLVLN"
BASE COUNT          288 a          519 c          502 g          276 t
ORIGIN      
        1 cattcaacac cagcatgtct atgccgccgt agcgcttcac cgccgcgccc agcgccgcgt
       61 cgaccgcctt cgcgtcggtg acgtcgcagg caataccggc gaaatccggg cgcatccaca
      121 tggtttcgat tgcggcgttg cgatcgagac ccactaccgc cgcgcctcgt ttcaaaaacg
      181 cctcgacgca ggcctttccg atccctgagg cggcgccggt caccagcacg acttcgcccg
      241 caaatggtgc cggcgcgccc gccttgcgca gcttcgcttg ctccagatcc cagtactcga
      301 tatcgaagat gtcgcgctcg ccgaccgcct gccagccgcc cagcgcctcg gcacgcaaga
      361 tgacgtcgat cgtgtgatcg tagagctcct cgatgatcgc cgcatcccgc gccgtgcggc
      421 cggccgcggc gaatccaagc gtcggatcca gcaccatgcg tggcgcgggg tccagcatgg
      481 ttttcttctc tttcgcctgc gcagcttggc gctcgaagta gtcccggtag ccgcgcgcgt
      541 acgcctgcac gtcgaatccg agcattggca gaggcttggt gcggataacg tgatccggtg
      601 tcgccggccc gcgctgtgaa aggcggctca acgccgagtg ctgcgcgaat gaaaggaact
      661 tcggggaatc gttgaccctc aagatcaacg gagcctcagc caagtcggaa atcgcacgcc
      721 gcaacccggc gattgcttca cgcgaaatgc tcgatgacct cggcgaaggc agcgacacgc
      781 tccacgcctt gttcgccgcg aggtaatcct cggccatcga cacgagcccg atcatgcgct
      841 cgtaggactc tttcgcgtcg gcgccgaacg agaacacgcc gtgcgacaac aagaccatgc
      901 cgatggtctg cgggcccgat tgtgcggcaa actcgcgcgc gcaatgagcc gcgagatcga
      961 atccggccat gatgtaggga atcacgacga ccttgtcgcc gtagatctcg cgcacgcgct
     1021 gctcgccgcg cggcgcgttg ctgatcgata gcaccgcgtc ggcgtgagtg tgatcgacgt
     1081 aatcatgcgg cagaatcgcg tgcaggatcg tctccacgga gggcgagggc gcgcccgcgc
     1141 gcaacatatg cgtgttgagc tcgttgacca tctgcgggtc ggacagcgcc ggcagctgcg
     1201 ccagacgccg gacgtaatcg agtgcgacgg gggtgaatcc ggcggcctcg atcgtctcga
     1261 gatcccatcc gctgcctttg acatagagca cgtcttcgga cgcgccgaag aggttcgtct
     1321 ggcgaagttt taccgaagtg tttccgccgc cgtgaaggac gagcgaccgg tccgttccca
     1381 gcagccggga ggtgtagacc cgcgggccga gcgggccgga atagcgggcc gcgtcggcgt
     1441 cgttccagag actgcgcatg gctatcctgc ggaaaaacgt gaaattatac tttcacctca
     1501 tgatccgaat cgagctatcc gcgtggtccg aggcgcgcgt cgaggcgatg cgcatccgcg
     1561 gaaccgtgtt cgtagaggag caggg
//
LOCUS       DASRVO010000254         1350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_111637, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000254 DASRVO010000000
VERSION     DASRVO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111637"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="pgi"
                     /locus_tag="VFF05_03120"
     CDS             complement(<1..656)
                     /gene="pgi"
                     /locus_tag="VFF05_03120"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462069.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZW50824.1"
                     /translation="MPGRSELPAWKPLAAHADALARTHLRELFAVDAERGERLTIDAA
                     GWYLDYSKNRIIDETVRLLVELADGCGVRARIDAMWRGDKINVTEQRAVLHVALRAQP
                     GEHFIVDGEDVVPEVHATLARMAAFSESVRSGEWKGYAGKPIRAVVNIGIGGSDLGPV
                     MAYEALRHYSQRDLQFRFVSNVDGTDFREAVRDLDPARTLFVVCSKTFTTQETLANAR
                     A"
     gene            complement(659..1138)
                     /locus_tag="VFF05_03125"
     CDS             complement(659..1138)
                     /locus_tag="VFF05_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HZW50825.1"
                     /translation="MSAVAEIQASPLPGRFAYLMGLYAENYHRLARVFAPQELGPGSF
                     ISSVDDGLDVRLDMVERHRYTLELHLTYCMTDARTGGPAPAAWLRMYRDAHVAEVTHC
                     HPGQRLWRELGPFAPAKTVFQHRMRMATFLNRWLEYLAEQGHSRATLLPLAALPACA"
BASE COUNT          242 a          432 c          443 g          233 t
ORIGIN      
        1 gcgcgtgcat tggcgagggt ttcctgcgtg gtgaaggttt tcgagcagac cacgaacaag
       61 gtccgcgcgg gatcgagatc gcgcacggct tcgcggaaat ccgtgccgtc gacattggag
      121 acaaaacgga attgcagatc acgctggctg taatgacgca gcgcctcgta ggccatcacc
      181 ggtcccaggt ccgatccgcc gatgccgata ttgacgaccg cccgaatcgg tttgccggcg
      241 taccccttcc attcgccgct gcgcacgctt tcgctgaatg cggccatgcg cgccagcgtg
      301 gcatggactt ccggcaccac gtcctcacca tcgacgatga aatgctcgcc aggttgcgca
      361 cgcaacgcga catgcaggac ggcgcgctgt tcggtgacat tgatcttgtc gccgcgccac
      421 atcgcatcga tgcgcgcgcg cacaccgcag ccatcggcga gttcgacgag caggcgcacg
      481 gtttcgtcga tgatgcggtt cttggaataa tccagatacc agccggccgc gtcgatggtc
      541 aggcgctcgc cgcgttcggc atcgacggcg aacagctcgc gcagatgcgt tcttgccagc
      601 gcatcggcat gcgcggcgag tggcttccag gcgggcagtt cgctgcggcc gggcatgctc
      661 aggcgcaggc cggcaaggcg gcgagcggca gcagggtcgc gcgagaatgg ccctgctcgg
      721 cgagatattc gagccagcgg ttgaggaatg tcgccatgcg catacgatgc tggaacacgg
      781 ttttcgccgg tgcgaacgga ccgagctcgc gccacaggcg ctggccggga tggcaatgcg
      841 tgacctcggc cacgtgggcg tcgcggtaca tgcgcagcca tgccgccggc gccggcccac
      901 cggtgcgcgc atcggtcatg caataagtca gatgcaattc gagcgtgtaa cgatggcgct
      961 cgaccatgtc caggcgcaca tcgagcccgt cgtcgacgga tgaaatgaaa ctgcccggcc
     1021 ccagttcctg cggtgcgaat acgcgcgcca ggcgatgata gttttccgca tacaagccca
     1081 tcaggtacgc gaagcgcccc ggcagcggac tggcctgaat ttcggcaacg gcggacatgc
     1141 gtcaatcata gcattcgccc tcctcgcaac cagtctgtac cggcgcacgc aatgcgccta
     1201 tgctcggcgg accgaacctc gcatcaatgg ccgtgcgagt gactgccgtg cgagtgtccg
     1261 ccgtgtggcg tgctgtgtga gccgctgccg tgccagccgc gaccgccgtg ccaatgactg
     1321 tggccgccgt agtagtggcc gccgtagtag
//
LOCUS       DASRVO010000255         1456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_319685, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000255 DASRVO010000000
VERSION     DASRVO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319685"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /gene="purM"
                     /locus_tag="VFF05_03130"
     CDS             <1..728
                     /gene="purM"
                     /locus_tag="VFF05_03130"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017118881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZW50826.1"
                     /translation="FFLDYFATGKLHVDTTVAVVGGIAKGCELAGCALIGGETAEMPD
                     MYPPGEYDLAGFNVGAVEKSALIDGTKIRAGDAIVGIASSGPHSNGYSLIRKIAARAG
                     NPFDLDLAGTKLADALMAPTTIYVKPVLEFLKSHAVHGMAHITGGGLTENIIRVVPDA
                     LGLALDTSTWKLPAVFDWLRREGNVPQVEMWRTFNCGIGYTLIVPRDAANATIAALEK
                     LGLPAWTIGEIVPARGDERVHIV"
     gene            736..1395
                     /gene="purN"
                     /locus_tag="VFF05_03135"
     CDS             736..1395
                     /gene="purN"
                     /locus_tag="VFF05_03135"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZW50827.1"
                     /translation="MRNVVVLASGRGSTFAALLDAQRHGALPIRIRALLSDKPAAPAL
                     DIARDAGIDAIALAPRDFADRAAFDRAVFARAADFHPDLIVLAGYMRVIDAAVVNEWR
                     GRMINIHPSLLPKYPGLHTHARVLEAGDRIHGSSVHFVTAQLDGGPVIAQAELPVHPD
                     DTPDTLAARLLPMEHRLLAACVDSIARGRIALGSAGVELDGVLFAAPLRLQIDGSLRT
                     A"
BASE COUNT          238 a          517 c          452 g          249 t
ORIGIN      
        1 tgttcttcct cgactacttc gccaccggca agctgcatgt cgataccacg gtcgccgtgg
       61 tcggcgggat cgccaaaggc tgcgaactgg ccggctgcgc cctgattggc ggcgaaaccg
      121 ccgagatgcc ggacatgtat cctccgggtg aatacgacct ggccggtttc aacgtcggcg
      181 cggtggaaaa atccgcgctg atcgacggca cgaaaatccg cgcgggcgac gccatcgtcg
      241 gcatcgcctc cagcggaccg cattccaacg gctattcgct gatccgcaag atcgctgcac
      301 gcgccggtaa tccattcgat ctggacttgg cgggcacgaa gctggccgat gcgctgatgg
      361 cgccgaccac gatctacgtg aagcccgtcc tggaattcct gaaatcgcat gccgtgcacg
      421 ggatggccca tatcaccggc ggcggcctga ccgaaaacat cattcgcgtg gtaccggacg
      481 cgctcggcct cgcgctggat acctcgacct ggaagctgcc cgccgtattc gactggttgc
      541 ggcgcgaagg caatgttcca caagtggaaa tgtggagaac gttcaactgc ggcatcggct
      601 acaccctgat cgtgccgcgc gatgccgcga acgccaccat cgccgcgctg gaaaagctcg
      661 gcttgccggc gtggaccatc ggcgaaatcg tgccggcgcg cggcgacgag cgcgtgcaca
      721 ttgtctgatt caggcgtgcg caacgtcgtc gtcctcgcct ccggccgcgg cagcaccttc
      781 gccgcgctgc tcgatgcgca gcggcacggc gcgctgccga tccgcatccg cgccctgctc
      841 tcggacaagc ccgcagcgcc cgcgctggac atcgcgcgcg acgccggcat cgacgccatc
      901 gcactcgcgc cacgcgattt tgccgatcgc gccgccttcg accgcgccgt gttcgcgcgc
      961 gccgcggact tccatcccga tttgatcgtg ctcgccggct acatgcgcgt gatcgacgcg
     1021 gcggtcgtga acgaatggcg cggccgcatg atcaacatcc atccttcgct gctgcccaaa
     1081 tatccgggat tgcacacgca tgcgcgtgtg ctcgaagccg gcgaccgcat ccacggctcc
     1141 agcgtgcact tcgttaccgc ccaactcgac ggcgggccgg tgatcgcgca ggccgaactg
     1201 cccgtccacc ccgatgacac gcccgacacg ctggccgcac gcctgctgcc aatggagcat
     1261 cgcttgctcg cagcttgcgt cgattcgatt gcgcgcggac gcatcgcact gggatctgcc
     1321 ggcgtcgaac tcgacggcgt gctgttcgcc gcgccgctgc gcctgcaaat cgacggcagc
     1381 ctgcgaacgg cgtgaggtcg acggaccgat tcccggcttg ccctggcgcc cggttcagtt
     1441 cctcggcatc gactgc
//
LOCUS       DASRVO010000256         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_319702, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000256 DASRVO010000000
VERSION     DASRVO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319702"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFF05_03140"
     CDS             <1..316
                     /locus_tag="VFF05_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW50828.1"
                     /translation="PVLLEWAVEVLTKNAIDALAGRGGIVRLSVEPGPEGAVVIRVAD
                     NGPGVPRELRSRVFEPGFSTKKSGWGIGLSLAKRIVEENHGGKLVLAPSEQGAAFDII
                     LH"
     gene            322..>1008
                     /locus_tag="VFF05_03145"
     CDS             322..>1008
                     /locus_tag="VFF05_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZW50829.1"
                     /translation="MLLEEQLTAGLNPAQREAVLHVEGPLLVLAGAGSGKTRVLTTRI
                     ARLIDVEGIDPRHILAVTFTNKAAGEMRERIGRLLGNDPAGMWVGTFHAIGARMLRAS
                     AHLVGRTPAFTIYDQDDSLSVIKRIMERHQISAKQFTPRAIQSALSDAKNALVTPAEY
                     ERLAMDPFARAVVPVYRSLGDALQLANAVDFDDLLVLPVRMLQQHADKLASYRARFRY
                     ILVDEYQDTNR"
BASE COUNT          176 a          316 c          340 g          176 t
ORIGIN      
        1 ccccgtgctc ctggaatggg cggtcgaggt gctgaccaag aacgcgatcg acgcactcgc
       61 cgggcgcggt ggaattgtcc ggctgtcggt cgagccgggg ccggagggtg ccgtcgtgat
      121 acgcgttgcc gacaatggac cgggggttcc gcgcgagctt cggtcgcgcg tgttcgagcc
      181 cggcttctcg accaagaaga gcggctgggg cattggcttg tcgctcgcca aacgcatcgt
      241 cgaggagaat cacggcggca agttggtgct cgcgccatcg gagcagggag cggcgttcga
      301 cattattctt cactgatgtc gatgctgctc gaggaacaac tgaccgccgg cctcaatccg
      361 gcgcagcgcg aagccgtgct gcacgtcgaa ggaccactgc tcgtgctcgc cggcgcggga
      421 tcgggaaaga cgcgcgtgct gaccacgcgc attgcccgat tgatcgacgt cgaggggatc
      481 gatccacggc acatcctcgc cgtcacgttc acgaacaaag cggcgggcga gatgcgcgag
      541 cgcatcggcc gcttgctggg caacgatccc gccgggatgt gggtcggaac gtttcacgcg
      601 atcggcgcgc gtatgctgcg cgcatcggcg catctcgttg ggcggacgcc tgctttcacc
      661 atctacgatc aggacgattc gctcagcgtg atcaagcgaa tcatggagcg acaccagatc
      721 tcggcgaagc agttcacgcc gcgcgcaatc cagtcggcgc tgtccgacgc caaaaatgcc
      781 ctcgtcacgc cggccgagta cgaacgcctg gcgatggatc cgttcgccag ggccgtcgtg
      841 ccggtgtacc gctcgctggg cgacgcgctg caactcgcga acgcggtcga cttcgacgat
      901 ctgctggtgc ttccggtgcg gatgctgcag cagcacgccg acaagctggc gagctaccgc
      961 gcgcgctttc gctacattct ggtcgatgaa tatcaggata cgaatcgc
//
LOCUS       DASRVO010000257         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_294344, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000257 DASRVO010000000
VERSION     DASRVO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294344"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VFF05_03150"
     CDS             complement(<1..994)
                     /locus_tag="VFF05_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HZW50830.1"
                     /translation="MSEPQIPLEPPRRRAALAFIFVTVLIDILSFGIIIPVLPHLIIE
                     LVGGSIAKAAVWSSAFGTLFMLMQFVFSPIQGALSDRFGRRTVILISSFGLGIDFIVM
                     ALAPVLWLLFLGRAVSGVCAASFSTANAYIADITPHEKRAASFGLLGAAFGIGFIIGP
                     ALGGLLGHISIRLPFWAAAFLALVNFCYGWFVLPESLPPERRSARIDWGHANPLGSLL
                     LLRRYPQVFALAAVFFLLALAQFSINSTYVLYTDFRYGWGPQAVGYTLALVGLCTAIV
                     QAGLVRKLMPKLGERRMILAGLCFSVAGYLLFGFAPSAWIFVAAIPIFCLAGLAG"
     gene            complement(1062..1691)
                     /gene="folE"
                     /locus_tag="VFF05_03155"
     CDS             complement(1062..1691)
                     /gene="folE"
                     /locus_tag="VFF05_03155"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011052881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HZW50831.1"
                     /translation="MTKSGKPSLCAAAAPTSPAVTREQALDAVRTLLRWSGDEPTREG
                     LLDTPKRVVDAYGEWFSGYKEDPQEYLRRTFEEVAGYDEMIVLRGITFESFCEHHMAP
                     IIGRAHVGYVPDGKVVGISKLARVVDAFAHRFQVQEKLTAQIATCISEVLHPRGVGVV
                     VDAVHQCMTTRGVHKRGVSLVTSQMLGSFRKDARTRAEFLRFIDIDANR"
     gene            complement(1754..>2240)
                     /locus_tag="VFF05_03160"
     CDS             complement(1754..>2240)
                     /locus_tag="VFF05_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZW50832.1"
                     /translation="AQIFADEVSTPLGMSDTGFNFGNMNPQIAGGVSSTMLDYANVVQ
                     MIVQQGQWNGTAYLSAAHIADMQKDQTHGVPMLYSPDPFAYGYGYGEWRNSIDAQGNA
                     VQLSSTGKFATSPWVDNSTGVAAVFLTYSNYDLVKSDLRELWANVHAVVTDPIFSTGF
                     E"
BASE COUNT          469 a          706 c          710 g          355 t
ORIGIN      
        1 gcccggcgag accggccaga cagaaaatcg gaatggcggc gacgaatatc catgcggacg
       61 gtgcgaaccc gaacagcaga tatccggcga ccgagaagca cagcccggcg agaatcatgc
      121 ggcgttcgcc gagtttcggc atgagtttgc gcaccaggcc cgcctgcacg atcgccgtgc
      181 acagtcccac cagcgcgagc gtgtagccga ccgcctgcgg gccccagccg tagcggaaat
      241 cggtgtacag cacatacgtc gaattgatcg agaactgcgc cagcgcgagc aggaagaaca
      301 ccgccgccag tgcaaacacc tgcgggtagc ggcgcagtag cagcaacgat cccagcggat
      361 tggcatggcc ccagtcgatg cgcgcgctgc gtcgttccgg cggcagcgac tcgggcaaca
      421 cgaaccagcc gtagcagaaa ttgaccagcg ccagaaacgc cgcggcccag aacggcaggc
      481 gaatggaaat gtggccgagc aggccgccca gcgccgggcc gatgatgaag ccgatgccga
      541 acgcggcgcc gagcaggccg aaactggccg cgcgcttttc gtgtggcgtg atgtcggcga
      601 tgtaggcgtt cgccgtggag aaactcgccg cgcatacgcc ggataccgcg cggccgagaa
      661 acagcagcca cagtaccggc gccagcgcca tgacgatgaa atcgatgcct aggccaaagc
      721 tcgaaatcag gatcaccgtg cgccggccga agcgatccga cagtgcgccc tggatcggcg
      781 agaacacgaa ctgcatcagc atgaacaagg taccgaacgc gctcgaccac actgccgcct
      841 tggcgatgct gccgccgacc agttcgatga tgagatgtgg caacaccgga atgatgatgc
      901 cgaaactgag aatgtcgatc aggacggtga cgaagatgaa cgcgagcgcc gcgcgccggc
      961 gtggaggttc cagcgggatt tggggttcac tcatgaatgc gctcggaaaa gtcgggcatc
     1021 tgcgctaggt tcgaaaaaca aaatggtctt gaacacgcgc atcatctgtt cgcgtcgatg
     1081 tcaataaacc gcaggaactc cgcgcgcgtg cgcgcatcct tgcggaagct gccgagcatc
     1141 tggctggtga ccagcgacac gccacgcttg tgcacgccgc gcgtggtcat gcactggtgc
     1201 acggcatcga cgaccacgcc gacgccgcgc ggatgcagca cctcgctgat gcaggtggcg
     1261 atctgtgcgg tcagcttctc ctgcacctgg aaacggtggg caaatgcatc gaccacgcgc
     1321 gccagtttgg aaatccccac gaccttgccg tccggaacgt agccgacgtg cgcgcgaccg
     1381 atgatcggcg ccatgtggtg ctcgcagaac gattcgaacg tgatgccgcg cagcacgatc
     1441 atctcatcgt agccggcgac ctcctcgaac gtgcggcgca ggtactcctg cggatcttcc
     1501 ttgtagccgg aaaaccattc gccataggcg tccacgaccc gcttgggcgt gtcgagcagg
     1561 ccctcgcggg tgggctcgtc gccggaccag cgcagcagcg tgcgcaccgc gtccagcgcc
     1621 tgctcgcgcg tgaccgctgg cgaggttggc gcggcggcgg cgcacaggga aggtttgccg
     1681 gatttggtca tgacggcact cgcgcggaaa aagacgcaac agtgtaccgc gtcgaccgga
     1741 tcggcgcgcc cgcttattcg aaaccggtgc tgaagatcgg atcggtcacc acggcatgca
     1801 cgttcgccca cagttcgcgc aaatcgctct tgaccagatc gtaattgctg taggtcagga
     1861 acacggcggc cacgccggtc gaattatcca cccacggcga cgtggcgaac ttgccagtgc
     1921 tcgacagctg caccgcattg ccctgcgcat cgatcgaatt gcgccactcg ccgtagccgt
     1981 agccgtaagc gaaaggatcg ggcgaataaa gcatcggcac gccatgcgtc tgatccttct
     2041 gcatgtcggc gatgtgcgcg gcggaaagat aggccgtgcc gttccattgg ccttgctgca
     2101 cgatcatttg caccacgttc gcgtagtcga gcatcgtcga actcacgccg cctgcgatct
     2161 gcggattcat gttgccgaaa ttgaaacccg tgtcggacat gcctagcggt gtgctcactt
     2221 cgtccgcgaa gatctgcgcc
//
LOCUS       DASRVO010000258         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_267969, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000258 DASRVO010000000
VERSION     DASRVO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267969"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1149
                     /locus_tag="VFF05_03165"
     CDS             <1..>1149
                     /locus_tag="VFF05_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZW50833.1"
                     /translation="AVFSADGTLVANAPHMPVHLGSMDRAVETIIRENEGRIAPGDVY
                     AINAPYNGGTHLPDITVCTPVFDDKKKSILFWVASRGHHADVGGISPGSMSPNATNIE
                     QEGVLFDNFKLVDRGRFREKELMEALTGAKYPARNPIQNVNDIKAQIAANEKGVAELR
                     KMVTQFTMPVVKAYMRHVQDNAAESVRRVIDRLHDSEYAFEMDQGTVIKVKITVDKKK
                     REATVDFTGTSAQQPTNFNAPEPVTRAAVLYVFRVMVDDDIPMNAGCLRPINIVIPPH
                     SMLSPEYPAAVVAGNVETSQAVTDTLFGALGALAAAQGTMNNLNFGNARYQYYETICS
                     GSPAGPGFNGTDAVHTHMTNTRLTDPEVLEFRYPVVLEDFHIRQGSGGR"
BASE COUNT          232 a          378 c          357 g          182 t
ORIGIN      
        1 gcggtgttct cggcagacgg cacgctggtg gcgaatgccc cgcacatgcc ggtgcatctc
       61 ggaagcatgg accgcgcggt ggagaccatc atccgcgaga acgaaggccg tatcgcgccc
      121 ggcgacgtct acgccatcaa cgcgccctat aacggcggca cgcatctgcc cgacatcacc
      181 gtgtgcacgc cggtgttcga cgacaagaag aaatcgatcc tgttctgggt tgcctcgcgc
      241 gggcaccatg ccgatgtcgg cggcatctcg cccggctcga tgtcgccgaa cgcgaccaat
      301 atcgagcagg aaggcgtgct gttcgacaat ttcaagctgg tcgaccgcgg ccgcttccgc
      361 gagaaggaat tgatggaggc gctcaccggc gcgaagtatc cggcgcgcaa tccgatccag
      421 aacgtcaacg atatcaaggc gcagatcgcc gccaacgaga agggcgtcgc cgaactgcgc
      481 aagatggtga cgcagttcac catgccggtc gtcaaggcct acatgcggca cgtacaggac
      541 aatgcggccg aaagcgtgcg ccgcgtcatc gaccgcctgc acgacagcga atacgcgttc
      601 gaaatggacc agggcaccgt gatcaaggtg aagatcacgg tcgacaagaa gaagcgcgag
      661 gcgaccgtcg acttcaccgg cacctcggcg cagcagccga caaacttcaa tgcgccggaa
      721 ccggtgacgc gcgccgcggt gctctacgtg ttccgcgtca tggtcgacga cgacattccg
      781 atgaacgccg gctgcctgcg gccgatcaac atcgtcattc cgccgcactc catgttgtcg
      841 ccggaatatc cggccgcggt ggtggcgggc aacgtggaga cctcgcaagc cgtcaccgac
      901 acgctgttcg gcgcgctcgg cgcgctcgcc gccgcgcagg gcaccatgaa caatctcaat
      961 ttcggcaatg cgcgctatca gtattacgag accatctgct ccggctcgcc ggccggtccc
     1021 ggcttcaacg gcaccgacgc cgtgcacacg cacatgacca atacgcgatt gaccgatccg
     1081 gaggtgctcg agttccgcta cccggtcgtg ctggaggatt tccatatccg ccagggttcg
     1141 ggcgggcgc
//
LOCUS       DASRVO010000259         1918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_137531, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000259 DASRVO010000000
VERSION     DASRVO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137531"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..511)
                     /gene="queD"
                     /locus_tag="VFF05_03170"
     CDS             complement(158..511)
                     /gene="queD"
                     /locus_tag="VFF05_03170"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016900974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HZW50834.1"
                     /translation="MDIFKRFHIEAAHRLPNVPAGHKCARLHGHSFGVEIHVSGTADA
                     HTGWVMDFADIKSAFAPIFAELDHNYLNEIPGLDNPTSERLAEWIWIRLKPVLPLLSK
                     VVVHETCTSGCAYSG"
     gene            complement(571..993)
                     /locus_tag="VFF05_03175"
     CDS             complement(571..993)
                     /locus_tag="VFF05_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012480480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HZW50835.1"
                     /translation="MTGMTTAMLDGFDALLRAALDQDEPQRLLFVFARRELGSDATAA
                     QRASFVRGEGGHLQPCLCVDKTPEEIAGFAALVAESAQTGQPWDIVFVGGLAGRGGIA
                     PNSDEAAQPLRFMVNAINEGRIADFAAFDRDGDALTFV"
     gene            complement(990..1460)
                     /locus_tag="VFF05_03180"
     CDS             complement(990..1460)
                     /locus_tag="VFF05_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZW50836.1"
                     /translation="MNQRINYVAASPDGFKAMRQLQAHVDNCGLEHGLLELVKIRASQ
                     INACAYCLDMHSKDARAAGETEQRLYLLDAWREAPFYSPRERAALAWTEALTRIAGND
                     VADALYAEVRAQFSDKELVDLSLAIIAINGWNRLAIPFRSEPGHYQPGDHAKRT"
     gene            complement(1475..>1918)
                     /locus_tag="VFF05_03185"
     CDS             complement(1475..>1918)
                     /locus_tag="VFF05_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZW50837.1"
                     /translation="FVHGVGTGGTLIGVGQALRKANPNVRLFAMEPSESRTILCGEVA
                     LHQIEGISDGFVPGIFAKHRALVGETTSVSSADAVAMMRRLARTRGLFCGPSSGAHLI
                     AAQRVREQNPQLQTVVTIFDDEGEKYLHDFFMRPAASDHAPVPFH"
BASE COUNT          347 a          595 c          626 g          350 t
ORIGIN      
        1 cgccactata cacgaaattt gctgcgatgc aacatacccc gacaagaacc ccgggatgtt
       61 ctgataacat attgattatg aatatataaa atacatgcca tagaacataa aacgcgccag
      121 tccatcggct gatttgcgca atcgcacaat ccgacgctta tccggaatag gcgcaacccg
      181 aagtacaggt ttcgtggacc acgaccttgc tcaacaaagg cagtaccggc ttcaggcgta
      241 tccatatcca ttcggccaga cgctcgctgg tcggattgtc cagccctggg atttcgttga
      301 ggtagttgtg gtcgagttct gcgaagatcg gagcgaatgc cgatttgatg tcggcaaaat
      361 ccatgaccca tccggtatgt gcatccgccg tgccggacac gtggatctcc accccgaaag
      421 aatggccgtg caggcgcgcg cacttgtggc ccgcgggcac gttgggcagg cgatgagctg
      481 cttcgatgtg aaaacgcttg aagatgtcca tgaacgcggg gcttgtcgga ggcctcgtag
      541 cttaacggct tttcctggag cgcgggccgc tcacacgaac gtgagcgcgt cgccgtcgcg
      601 atcgaacgcc gcgaagtcgg cgatgcggcc ttcgttgatc gcattgacca tgaagcgcag
      661 cggctgggcg gcctcgtcgc tattcggagc gatgccgccg cggccggcaa gcccgccgac
      721 gaagacgatg tcccagggct ggccggtctg cgcggattcg gcgaccagcg cggcgaagcc
      781 ggcgatttcc tccggcgtct tgtccacgca caggcacggt tgcaggtggc cgccctcgcc
      841 acgcacgaag ctggcgcgtt gcgcggcggt ggcatcgctg cccagttcac ggcgcgcgaa
      901 cacgaacagc aggcgctgcg gttcgtcctg gtccagggcg gcgcgcaaca gcgcgtcgaa
      961 gccgtcgagc atcgcggtcg tcatgccggt catgtgcgct tggcgtggtc gcccggctgg
     1021 tagtggccgg gttcggagcg gaacggaatc gcgaggcggt tccagccgtt gatcgcgatg
     1081 atcgccaggg aaagatcgac cagttccttg tcgctgaact gcgcgcgcac ttcggcgtac
     1141 agcgcatcgg ccacgtcgtt gccggcaatg cgggtcaagg cttccgtcca cgccagcgcg
     1201 gcgcgttcac gcgggctgta gaacggcgct tcacgccagg cgtcgagcag gtacaggcgc
     1261 tgctcggttt cgccggcggc gcgcgcgtcc ttgctgtgca tgtccaggca ataggcgcag
     1321 gcgttgatct gcgaggcgcg aatcttcacc agttcgagca ggccgtgctc gagcccgcaa
     1381 ttgtcgacat gcgcctgcaa ctggcgcatc gccttgaagc catcgggcga ggccgcgacg
     1441 taattgatgc gttggttcac gtggatactc ctcgtcaatg gaacggcacc ggcgcatggt
     1501 cggacgccgc cgggcgcatg aagaaatcgt gcaggtactt ctcgccttcg tcgtcgaaga
     1561 tcgtcacgac ggtctggagt tgcggatttt gctcgcgcac gcgctgcgcg gcgatcaggt
     1621 gcgcgccgga actcggcccg cagaacagcc cgcgcgtgcg cgcgagccgg cgcatcatcg
     1681 ccacggcgtc ggcgctggac acgctcgtcg tttcgcccac cagcgcgcga tgcttggcga
     1741 aaatgcccgg cacgaaaccg tcggaaatgc cctcgatctg atgcagcgcg acttcgccgc
     1801 acaggatggt gcgcgattcg ctgggttcca tcgcgaacag gcgcacgttc ggattggcct
     1861 tgcgcaatgc ctggccgacg ccgatcaggg tgccgccggt gccgacgcca tgcacgaa
//
LOCUS       DASRVO010000260         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_268027, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000260 DASRVO010000000
VERSION     DASRVO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268027"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1033
                     /gene="msrP"
                     /locus_tag="VFF05_03190"
     CDS             44..1033
                     /gene="msrP"
                     /locus_tag="VFF05_03190"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HZW50838.1"
                     /translation="MRRDPSKNPPPSAITPEPVYRRRREVLKLLGLGATALAAGCGSE
                     AAPPPPAAPVTNPPGKPLIAARRVDDAGGAPQTTYQDATHYNNFYEFGTGKGDPAANS
                     GAFQPRPWSVEIAGLAEKTGRLTLEDILKGHALEERIYRMRCVEAWSMVIPWAGFPLA
                     DLLKRCKPLASAKYVAFTTVQRPAQMPGLAYPVLDWPYREGLRMDEAMHPLTLLAVGM
                     YGRELPNQNGAPLRLVVPWKYGFKGIKSIVKIEFVERQPQTTWNMSAADEYGFYSNVN
                     PAVDHPRWSQKTERRIAGTGFNLFGNRIATLPFNGYAEQVAHLYAGMDLRKYF"
     gene            1062..1697
                     /locus_tag="VFF05_03195"
     CDS             1062..1697
                     /locus_tag="VFF05_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HZW50839.1"
                     /translation="MMTFRDRIAAFKPLVFIACLLPLAHLVWDAVQGTLGTDPVAQLE
                     HRSGDWALRLLLATLAITPLRRISGWHKAIRFRRMLGLFAFFYVSVHLTIYLAIDLGG
                     FWSQLLGEIIKRPYITVGFAAWLLLIPLALTSTQAMMRRLGRNWQRLHRLIYPIALLG
                     VLHYFWLVKADHREPAVYLAIWVVLMMLRLPMKQMRARRQPPARMAVALKD"
     gene            complement(1687..>2611)
                     /locus_tag="VFF05_03200"
     CDS             complement(1687..>2611)
                     /locus_tag="VFF05_03200"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZW50840.1"
                     /translation="HHPAVLQSCACKVTAFVMRLCFPNNEHADVLIAPGDTAIGSAPD
                     STIVLTAPGVAPHHARLSVSERSIVLAAGEGGARIHVNARPVVEKAILRLGDVVSLDT
                     IKFTLKPDRDDSISTDVPGHVTPPPAAPADADDTATRVRQLPARVVLRGVSGSYFGKI
                     VPVRGRLLIGRGSDCGLILDEPEMSRHHAAIENAGDGIYLRDLGSANGTFVNGVLVRD
                     AVLHTDDQIAFDRNRFLLEAPSLPTRGDLAQEAAPAQAVNITQTMRAIDKPAAQPAVP
                     AEERNRNDIWWLIGAAALIAAGIAVLLYISL"
BASE COUNT          460 a          859 c          815 g          477 t
ORIGIN      
        1 tatgcgcgat tgccggtctc ctcggcacaa tcgggaaaaa cgcatgcgtc gagatccatc
       61 caaaaacccg ccgccatcgg caatcacgcc ggaaccggtt taccggcgtc ggcgcgaagt
      121 gttgaaactg ctcgggctgg gcgcaaccgc actggccgcc ggctgcggct ccgaggccgc
      181 ccctccccca cccgcggccc cagtgacgaa tcccccgggc aagccgctga tcgccgccag
      241 gcgcgtcgac gatgccggcg gcgcgccgca aaccacctat caagacgcaa cgcactacaa
      301 caatttctac gagttcggca ccggcaaagg cgatcctgcc gccaattccg gtgctttcca
      361 gccacgaccg tggagcgtgg aaatcgccgg ccttgccgaa aagaccggac gcttgacgct
      421 cgaagacatc ctcaagggcc atgcgctgga agaacgcatt taccgcatgc gttgcgtgga
      481 agcctggtcg atggtgattc cctgggccgg cttcccactt gccgacctgc tcaagcgctg
      541 caagccgctg gcgtcggcga agtacgtcgc tttcacgacg gtccaacgac cggcgcagat
      601 gcccggcctc gcctatccgg tgctcgattg gccctatcgc gaaggcctgc gcatggacga
      661 ggccatgcat ccgctgaccc tgctcgccgt gggcatgtat gggcgcgaac tgccgaacca
      721 gaacggcgcg ccgctgcgcc tggtcgtgcc gtggaaatac ggcttcaagg gcatcaagtc
      781 catcgtcaag atcgaattcg tcgagcgcca accacaaacg acctggaaca tgtcagccgc
      841 ggacgagtac ggcttctatt ccaacgtgaa ccccgccgtg gaccatccgc gctggagcca
      901 gaaaaccgaa cgccgcatcg ccggtaccgg tttcaacctg ttcggcaacc gcatcgccac
      961 cttgccgttc aacggctatg ccgaacaagt ggcgcacctg tatgccggca tggatctgcg
     1021 caagtatttt tgacgacggc gtttttgata cggcatcatt gatgatgact tttcgtgatc
     1081 gcattgccgc gttcaaaccg ctggtcttca tcgcgtgcct gctgcctctg gcgcatctgg
     1141 tctgggatgc agtgcaaggc actctgggga ccgatcccgt cgcccagctc gaacatcgca
     1201 gcggcgactg ggcattgcgc ctgctgctcg ccacgctcgc gatcactccg ctacgtcgga
     1261 tcagcggctg gcacaaggcg atccgtttcc ggcgcatgct cggcctgttc gcgtttttct
     1321 acgtgagcgt gcacttgacg atttatctcg ccatcgacct tggcggtttc tggtcgcaat
     1381 tgctcggcga aatcatcaag cgaccgtaca tcacggtggg cttcgcggcg tggctgctgc
     1441 tgattccgct ggccttgacc tcgacacagg cgatgatgcg ccgcctcggt cgcaactggc
     1501 agcgtctgca tcggctgata tatccgatcg ccctgcttgg cgtgctgcac tacttctggt
     1561 tggtcaaggc cgaccaccgc gagccggcgg tgtaccttgc gatctgggtc gtactgatga
     1621 tgctgcggtt gccgatgaag cagatgcgtg cacggcgcca gccaccggcc aggatggccg
     1681 tggcactcaa agactgatgt agagcaacac cgcgattccg gcggcaatga gcgcggccgc
     1741 gccgatcagc caccagatgt cgttgcggtt gcgttcctcg gccggcacgg cgggctgagc
     1801 cgccggtttg tcgatcgcgc gcatggtctg ggtgatgttg accgcctgcg caggcgcagc
     1861 ctcctgcgcg agatcgccgc gcgttggcaa gcttggcgcc tcgagcagaa agcggttgcg
     1921 gtcgaatgcg atctggtcgt cggtatgcag caccgcatcg cgcaccagca cgccgttgac
     1981 gaaggtgcca ttggccgaac ccaggtcgcg cagataaatg ccgtcaccgg cgttttcgat
     2041 ggccgcgtga tgacgcgaca tctccggctc gtcgaggatc aacccgcaat cgctgccgcg
     2101 accgatcagc aggcgccccc gcaccggcac gatcttgccg aaatagctcc ccgaaacgcc
     2161 acgcagcacg acgcgcgccg gcaattgccg cacccgtgtc gcagtgtcgt ccgcatccgc
     2221 cggcgcggcg ggcggcggcg tgacgtggcc gggaacgtcg gtgctgatgc tgtcgtcgcg
     2281 gtcgggcttg agcgtgaatt tgatcgtgtc gaggctgacc acgtcgccca ggcgcaggat
     2341 cgccttttcg acaacgggac gtgcattgac atggatgcgc gcaccgcctt cgccggcggc
     2401 gagcacgatg ctgcgctcgc tcacgctcag ccgcgcatga tgcggggcga cgccaggcgc
     2461 ggtcaacacg atcgtgctgt cgggtgcgga gccgatggcg gtatcgccgg gagcgatcag
     2521 cacatccgca tgttcgttgt tcgggaagca cagacgcatg acgaacgccg tcaccttgca
     2581 ggcgcatgac tgtagcacag ccggatggtg c
//
LOCUS       DASRVO010000261         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_345248, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000261 DASRVO010000000
VERSION     DASRVO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345248"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VFF05_03205"
     CDS             <1..1248
                     /locus_tag="VFF05_03205"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HZW50841.1"
                     /translation="FVALCVREAGKTLAAAVSEVREAADMCRYYAAMARKLMGKPDAL
                     TGPTGESNQLSLHGRGVFVCISPWNFPLAIFTGQVAAALAAGNAVIAKPADQTVLIGH
                     AAVKLLHEAGVPEAVLQFVPGKGSMIGNTLLTKPEVAGVCMTGSTETAWTINRTLAAR
                     NAQIASLIAETGGQNALIADSSALPEQLVKDAIASAFDSAGQRCSAARVLFVQDDIAD
                     KVIAMLAGAMDELKVGDPALLSTDVGPVIDEPSRKSLADHAARMDAEAKLINEVKLDA
                     SCANGTFLAPRAYELKSLSQLTHEVFGPILHVIRWKASELDKVIDAINATGFGLTLGI
                     HSRIDSTIAHISQRAKVGNCYVNRNQIGAVVGVQPFGGEGLSGTGPKAGGPHYLLRFV
                     TERTLTINTTAAGGNASLLTLGE"
     gene            complement(1352..1588)
                     /locus_tag="VFF05_03210"
     CDS             complement(1352..1588)
                     /locus_tag="VFF05_03210"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZW50842.1"
                     /translation="MLAVEEAATAPPGSGGANKLCKQRVSIMLDASIIAFFKAKAGDR
                     GYQTLINQAQHRAMSGEQIEAALRRVIREGLKAA"
     gene            complement(1575..2003)
                     /locus_tag="VFF05_03215"
     CDS             complement(1575..2003)
                     /locus_tag="VFF05_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50843.1"
                     /translation="MSRFLTRLALALTCLALPALALAGGKPIPAGGGDLGKVFQANLA
                     AMQKGDTDAMLATVSKAQAAKFREQQKDPQFAAMLGMMKAFAPKSVTVTGGEDFGDHA
                     ELTLVGVEQSGGKSTGTAKMVKEDGQWKVEKTSMSSSAGG"
     gene            2151..>2400
                     /locus_tag="VFF05_03220"
     CDS             2151..>2400
                     /locus_tag="VFF05_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZW50844.1"
                     /translation="MYLEFYGLKEAPFSITPDPRYVFLSERHRDALAHLLYGIGKGGS
                     GGFVQLTGEVGTGKTTLCRLLLEQLPENTRVALVLNPKL"
BASE COUNT          438 a          814 c          714 g          434 t
ORIGIN      
        1 ttcgtcgcgc tgtgcgtgcg cgaagccggc aagacgctcg cggccgcggt ttccgaagtg
       61 cgcgaagccg ccgacatgtg ccgttattac gcggccatgg cgcgcaagct catgggcaaa
      121 cccgatgcgc tcaccggtcc gaccggcgaa tcgaatcagc tttcgctgca cggccgcggc
      181 gtgttcgtgt gcatcagtcc gtggaatttc ccgctcgcca tcttcaccgg ccaggtcgcc
      241 gccgcactcg ccgccggcaa tgccgtgatc gccaagccgg ccgaccagac cgtgctgatc
      301 ggccatgccg cggtgaagct gctgcacgaa gccggcgtgc ccgaagccgt gctgcaattc
      361 gtgcccggca agggctcgat gatcggcaac acgttgctga ccaagcccga ggtcgccggc
      421 gtgtgcatga ccggctccac cgaaaccgca tggacgatca accgcacact cgccgcgcgc
      481 aacgcgcaga tcgcgagtct gatcgccgaa accggcggcc agaacgcgtt gatcgccgat
      541 tcgtccgcgc tgcccgagca attggtgaaa gatgccatcg catccgcgtt cgactccgcc
      601 ggccagcgct gctccgccgc gcgcgtgctg ttcgtgcagg acgacatcgc cgacaaggtg
      661 atcgccatgc tcgccggcgc gatggatgaa ttgaaagtcg gcgacccggc gctgctgtcc
      721 accgacgtcg gcccggtcat cgacgaaccc tcgcgcaaga gtcttgccga tcatgccgcg
      781 cgcatggacg ccgaagccaa gctcatcaat gaagtgaagc tcgacgcatc gtgtgcgaat
      841 ggcaccttcc ttgcgccgcg cgcttacgag ttgaagtcgt tatcgcaact cacccacgaa
      901 gtgtttggcc cgatcctgca cgtgatccgc tggaaggcca gcgaactcga caaggtcatc
      961 gacgcgatca atgcgaccgg ctttggcctg acgctcggca tccacagccg catcgattcc
     1021 accatcgcgc atatttctca acgcgcaaaa gtcggcaact gctacgtcaa ccgcaaccag
     1081 atcggcgccg tcgtcggcgt gcagccgttc ggcggcgaag gactctccgg caccggcccc
     1141 aaggccggcg gcccgcacta cctgctgcgc ttcgtcaccg aacgcacgtt gacgatcaac
     1201 acgactgccg ccggcggcaa tgcgtcgttg ttgacccttg gggaataatt cagctcgtca
     1261 ttccggcgaa agccggaatc cagcgtacta gtgttgtttc tccgcgtcct gcgcatttcc
     1321 gctcgcattc agcgagcgtg ccacttgtca gctacgccgc cttcaacccc tcgcgtataa
     1381 cccgccgcag cgcggcttcg atctgttcgc ccgacatcgc gcggtgctgc gcctgattga
     1441 tcagggtctg ataaccgcgg tcgccagctt tggccttgaa gaaggcgatg atcgacgcgt
     1501 cgagcatgat cgacacgcgc tgcttgcaga gcttgttggc gccgccagaa cccggcggcg
     1561 ccgttgccgc ttcctcaacc gccagcactc gacgacatcg acgtcttctc gactttccac
     1621 tggccgtctt ccttgaccat ctttgcggta ccggtcgact tgccgccgct ctgctcgacg
     1681 ccgacgaggg tcaattcggc gtgatcgccg aaatcctcgc ctccggtgac ggtaacgctt
     1741 ttgggcgcga atgccttcat catgcccaac atggccgcga actgcgggtc cttttgctgc
     1801 tcgcgaaact tcgccgcctg ggccttggag accgtggcga gcatcgcgtc ggtatcgcct
     1861 ttttgcatcg ccgcgagatt ggcctggaac accttgccga gatcgccgcc gccggccgga
     1921 atcggcttgc cgccggccag cgccagtgcc ggcagggcca ggcaagtcag ggcgagcgca
     1981 agacgcgtga gaaatctgga catggcaatt ctccacatac ccgcattccg ggcacttggg
     2041 gaaacgtaaa acgcggccgc aaacgaacgc acgggacgcc aagatgacgc ttgtcgacta
     2101 agatcgttga aaacacgaac tttcaacccc ttccggggta aagtaaccgc atgtacctcg
     2161 agttctacgg gctgaaagaa gcccccttct cgatcacccc cgatccacgc tacgtgttcc
     2221 tgagcgagcg ccaccgggac gcgctcgcgc acctgttgta cggcatcggc aagggcggca
     2281 gcggcggttt cgtgcagctg accggcgagg tcggcacggg caagaccacg ttgtgccggc
     2341 tgctgctgga acagttgccg gaaaacacgc gcgtggcgct ggtactcaat ccgaagctgt
//
LOCUS       DASRVO010000262         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_8046, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000262 DASRVO010000000
VERSION     DASRVO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8046"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFF05_03225"
     CDS             <1..374
                     /locus_tag="VFF05_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZW50845.1"
                     /translation="DMGEPVKICYLAEQMIRLAGKVPERDVAIVYTGLRPGEKLFEEL
                     FHDHENYRATEHAKIFLARPRSVSWELLTAQLRQAAQSVRDYDEDALRACLTSLLPDF
                     SWRDEADSADIVPIARHVEGA"
     gene            375..1262
                     /gene="galU"
                     /locus_tag="VFF05_03230"
     CDS             375..1262
                     /gene="galU"
                     /locus_tag="VFF05_03230"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HZW50846.1"
                     /translation="MNAALRKVVFPVAGLGTRFLPASKVVAKEMLPVLDKPLIQYAVD
                     EAVNAGADTLVFVTNRYKHAIADYFDTAYELERKLESSGKHELLALVRNVIPRHVRCV
                     FVTQSEALGLGHAVLCAKPVIGDEPFGVILPDDLIWTSPAQAGGAGALRQMAEFAARE
                     NAGAIAVEEVPHADTQKYGIVDAEKTNDRAARIRHVVEKPKPEVAPSNLAIVGRYVLP
                     RRIFELLEKTKPGAGGEIQLTDAIAALLGEGKVYAYRFEGTRFDCGSKLGLVKATLHF
                     ALQDPALADTTRAFVGGAS"
     gene            complement(1259..1738)
                     /locus_tag="VFF05_03235"
     CDS             complement(1259..1738)
                     /locus_tag="VFF05_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50847.1"
                     /translation="MRTVLHVDIGSHERCAMLGVMQIQKADPLYRRRVLAILAITAIA
                     MLALVFGFTHWLQHVTQDMDAARLLAWIKRLIAGCMVLMTLCLLLLGRHLLLRGRRIL
                     RDRRYPANDARPLRDTPMREGDEAVHIGKASRVAGLIACLLGVAVAVTGWFWVAQFG"
BASE COUNT          356 a          572 c          603 g          333 t
ORIGIN      
        1 tcgacatggg cgagccggtg aagatctgct atctcgccga gcagatgatc cgcctcgccg
       61 gcaaggttcc ggagcgcgac gtcgccatcg tctacaccgg tttgcgtccg ggcgagaaat
      121 tgttcgagga attgttccac gaccacgaga attatcgagc aacggagcac gccaagattt
      181 tccttgcgcg gccgcgcagc gtgtcgtggg aattgctcac cgcgcagttg cgccaggccg
      241 cgcagtccgt gcgcgattac gatgaggacg ccttgcgcgc ctgcctgacc agcctgttgc
      301 cggatttttc ctggcgcgac gaagcggatt ccgccgatat cgtgccgatc gcacgtcatg
      361 tcgaaggagc ttgaatgaac gcagcgctgc gcaaggtcgt gtttcccgtg gccggacttg
      421 gcacgcgctt cctgcccgcg tccaaggtgg tggcaaagga aatgctgccg gtactggaca
      481 agccgctgat ccagtatgcg gtcgatgagg ccgtgaacgc cggcgcggac acgctggtct
      541 tcgtgaccaa ccgctacaag cacgcgatcg ccgactattt cgacacggcc tacgaactgg
      601 aacgcaaact cgagagttcg ggcaagcacg agcttcttgc gctggtgcgc aacgtgattc
      661 cgaggcatgt gcgctgcgtg ttcgtgaccc agtccgaggc gctgggcctg ggtcatgccg
      721 tgctgtgtgc aaaacccgtg atcggcgacg agcctttcgg cgtgatcctg ccggacgacc
      781 tgatctggac gagtccagca caagctggcg gcgccggtgc gctgcgccag atggcggagt
      841 ttgcggccag ggaaaatgcc ggcgcgatcg cggtcgagga agtgccgcac gcagacacgc
      901 agaaatacgg catcgtcgat gcggaaaaga ccaatgaccg cgccgcgcgc attcgtcacg
      961 tcgtcgagaa acccaaaccc gaagtggcac cgtccaacct cgccatcgtc ggtcgctacg
     1021 tgctgccgcg ccgcatcttc gagttactgg aaaaaacgaa gccgggcgcg ggtggtgaga
     1081 tccagcttac cgacgcgatc gccgctttgc tcggcgaagg caaggtctac gcttatcgct
     1141 tcgagggcac gcgtttcgat tgcggcagca agctcggact ggtcaaggcg accctgcatt
     1201 tcgccttgca ggatccggca ctcgcagata cgacgcgtgc ctttgttggg ggcgcttcct
     1261 agccgaactg ggcgacccag aaccagccgg tcactgcaac cgccacgcca agcaggcagg
     1321 caatcaatcc ggcgacgcgg ctggctttgc cgatgtgcac ggcctcatca ccttcgcgca
     1381 tgggcgtgtc gcgcagcggg cgcgcgtcgt tggcaggata gcgccggtcg cgcaggatgc
     1441 ggcgtccgcg cagcagcagg tggcggccga gcagcaacag gcacagcgtc atcaggacca
     1501 tgcagccggc gatcaggcgt ttgatccatg ccagcaaacg cgcggcatcc atgtcttgcg
     1561 tgacatgttg cagccaatgc gtgaagccga acacaagcgc cagcatggcg attgccgtga
     1621 tggccaggat ggccagcacg cggcggcgat atagcggatc ggccttctgg atttgcatga
     1681 cgccaagcat agcgcaacgc tcgtgcgagc cgatgtccac gtgcaacacg gtgcgcaacg
     1741 aactgtgcaa aacggctatg cttgcgcatc gattcggatg gagaccggca tgcgtaccgt
     1801 cgcattgttg attttgtcgg gcctgctcgc ggcttgccag agcgcaccga gccgtactgt
     1861 tccc
//
LOCUS       DASRVO010000263         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_137615, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000263 DASRVO010000000
VERSION     DASRVO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137615"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VFF05_03240"
     CDS             <1..350
                     /locus_tag="VFF05_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50848.1"
                     /translation="GVLYLLLNLRGVRARFVQAALALTSCAIVFAILSVPIVLAVGGK
                     PPTPETITPLQALLGMLALPIVVWKLIVDAHILRHSLNLPFAAGLLIALFWIIAELSF
                     GAAIGGPAAAAAA"
     gene            347..1744
                     /gene="mpl"
                     /locus_tag="VFF05_03245"
     CDS             347..1744
                     /gene="mpl"
                     /locus_tag="VFF05_03245"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017117067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZW50849.1"
                     /translation="MSGIVKLYILGICGTFMGGVAALARELGHDVAGCDANIYPPMSD
                     QLAALGVHLDSGYRAEHLDAAKPDLVIVGNALSRGNPVVERLLDARIPYISGPQWIGE
                     NVLASRRVFAVAGTHGKTTTTAMLAWILDACGRHPGFLVGGVPENFGVSARLGGGDFV
                     IEADEYDTAFFDKRSKFVHYRPSIAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPGN
                     GRLVVNAEDARLAEVLAMGVWTPVTTFGMATGDWQARLIHPDNGAAEGGRPADGSQFA
                     VLRDGRDVGIVRWNLLGRHNVMNALAALAAADSSGVDVATAIAALADFRSAKRRLEHI
                     GEHGGITVYDDFAHHPTAIATTLAGLRARVDKARILVGTEPRSNSMRLGAHADELAPS
                     LRDADAVVFLKRPELAWDAQSVIDGLGGRGQTAPTVDALIALLAESARKGDHVVFMSN
                     GGFEDAPRRFLRSLR"
     gene            1770..2360
                     /locus_tag="VFF05_03250"
     CDS             1770..2360
                     /locus_tag="VFF05_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZW50850.1"
                     /translation="MTATDIPLFPLNSVLFPGGVLELRVFERRYLDLVRDCARSGAGF
                     GVCLILHGREAGEPAVPAAVGTLARIVDFYTSDDGLLGIRADGGARFRVMHTRVRDNG
                     LVHGDVHFWPDEPVVQVPPEHALLATILERLLECMGGQDASGPRERYDDASWVGFRLA
                     EALPLENRERQHLLQMTDPLERLTQLMHYLPRFQHA"
BASE COUNT          406 a          862 c          837 g          487 t
ORIGIN      
        1 tcggcgtgct gtatctgctg ctcaacctgc gaggcgtgcg tgcccgtttc gtgcaggccg
       61 cgctggccct gaccagttgc gcgatcgtgt tcgcaatttt gagcgtgccg atcgtattgg
      121 ccgtcggcgg caaaccgccg acgccggaga cgatcacgcc attgcaggca ttgctcggca
      181 tgctcgcgct gccgatcgtg gtgtggaaac tgatcgtcga cgcgcacatc ctgcgccaca
      241 gcttgaacct tccgttcgcc gcagggctgc tgatcgcgct gttctggatc atcgccgaat
      301 tgagcttcgg tgcggccatc ggcggcccag ccgctgccgc agcagcctga gcggcattgt
      361 gaaattgtat attctgggaa tttgcggaac cttcatgggc ggcgtcgccg cgctggcgcg
      421 cgagcttggc cacgacgtcg ccggttgcga cgcgaacatc tatccgccga tgagcgatca
      481 gctcgccgcg ctcggcgtgc atctggattc cggctatcgc gccgaacatc tggatgcggc
      541 caaacccgat ctcgtcatcg tcggcaacgc gctgtcgcgc ggcaatcccg tggtcgaacg
      601 tctgctcgac gcgcgcattc cctacatttc cggcccgcaa tggatcggcg aaaacgtgct
      661 cgcgtcgcga cgcgtctttg ccgtcgccgg cacgcacggc aagaccacga cgacggcaat
      721 gctggcgtgg atcctcgatg cctgcggccg ccatcccggg tttctggtcg gcggcgtgcc
      781 ggagaatttc ggcgtgtcgg caaggcttgg cggcggcgat ttcgtcatcg aagccgatga
      841 atacgacacc gcgttcttcg acaagcgcag caagttcgtg cattaccggc cgagtatcgc
      901 gatcctcaac aacctcgaat acgaccacgc ggacatcttt cccgacgtcg ccgcgatcca
      961 gcgccagttc catcatctgg tgcgcaccgt gccgggcaac ggacgccttg tagtcaatgc
     1021 cgaggacgcg cgccttgccg aagtgttggc catgggtgtg tggacgccgg tcacgacgtt
     1081 cggcatggcc accggcgatt ggcaggcgcg cctgattcat cctgacaatg gcgcggccga
     1141 gggcggccgc cccgccgacg gatcgcaatt cgccgtgctg cgcgacggcc gcgatgtcgg
     1201 catcgtgcgc tggaatctgc ttggccggca caacgtcatg aatgcgctgg ctgcgctcgc
     1261 ggcggccgat tcgagcggtg tggatgtggc gaccgccatc gccgcgctcg ccgatttccg
     1321 cagcgccaag cgccgcctcg aacatatcgg cgagcacggc ggtataaccg tatacgacga
     1381 cttcgcgcat cacccgaccg cgatcgcgac gacgctcgcg ggcctgcgcg cacgcgtgga
     1441 caaggcgcgc attctggtcg gcacggaacc gcgctcgaac tcgatgcgcc tcggcgcaca
     1501 cgcggacgag ctcgcacctt ccttgcgcga cgccgatgcg gtggttttcc tcaagcgacc
     1561 cgagctggcc tgggatgcgc agagcgttat cgacgggctc ggcggccgcg gccagaccgc
     1621 gccgacggtc gacgcattga tcgcgctgct cgccgaatcc gcgcgcaagg gcgaccacgt
     1681 cgtgttcatg tccaacggcg gattcgagga cgcgccgcgg cgattcctgc gcagtttgcg
     1741 ctgacgccga tccggcctac acttcaggcg tgacggccac cgacatcccg ttgtttccgc
     1801 tcaacagcgt gctgtttccc ggcggtgtgc tcgaattgcg cgtgttcgaa cgtcgctacc
     1861 tcgacctggt tcgcgattgc gcgcgcagcg gtgccggttt cggcgtatgc ctgatcctgc
     1921 acggccggga agcgggcgaa ccggcggtgc cggcggcggt cggcacattg gcacgcattg
     1981 tggatttcta tacatccgac gatggcctgc tcggcatccg tgccgacggc ggtgcgcgct
     2041 tccgtgtcat gcacacgcgc gtacgcgaca acggcctcgt gcatggcgac gtgcatttct
     2101 ggccggatga acccgtcgtg caggtgccgc ccgaacatgc gctgctcgcg accatcctcg
     2161 agcgcctgct cgaatgcatg ggcggccaag acgcgagtgg gccacgcgag cgctacgacg
     2221 atgcgagttg ggtcggattc cgcctagccg aggcgctgcc gctggagaat cgcgagcgcc
     2281 agcatctgtt gcagatgact gacccgctgg aacgccttac ccagttgatg cactatctgc
     2341 cgcgcttcca gcacgcttga gagtgcgcat gacgacactt gtcggcacga cctcgtccat
     2401 cacttgtgcc gcgcgccacg tccctgcgcg cgcaggcctg cgcgaccgat cgttccggct
     2461 acgtggcagg cccgggtcgg aagcagcctg ccgattcgtt cctggattga tggcatgaat
     2521 gtaaaggatt tctcgcggcg tcgcgccgca ttccgcaaac ttcacgaatc gggttgcttc
     2581 atgctgccca at
//
LOCUS       DASRVO010000264         5479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_241434, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000264 DASRVO010000000
VERSION     DASRVO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241434"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3767
                     /locus_tag="VFF05_03255"
     CDS             <1..3767
                     /locus_tag="VFF05_03255"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HZW50851.1"
                     /translation="VLGLQGRARARLFVRRDKFGRFFSCMAFVPRDRFTADVRERIEG
                     LLKDTFHGERLDSTINVGESALARLHLIVRPKPGEKPEYDTAQLEERIGQIVRNWRDS
                     LRDELVRKHGEEKGSKLASRFGRALPAGYIEEVSAEVAAADVESIASLKDADDLRLSL
                     YRPGKDDSLRFKLFRYGAPISLSDALPLLENMGLKILSEHPYEMKLGDSAAVIQEFEA
                     QTAVSCGFDIEQIRDSFQASFERIWRGQAENDGFNKLILAANMGWRQVAMLRGLCKYI
                     LQTGAPFSQSYMEETLNRYPLIAGLLVELFEARFDPQREDPGKTVLEGARKRLRKELE
                     ILLPAEVAKAHPAFVDDLLNARGQTRDAQMQAIVAALRILLDRVASLDEDRILRGFLG
                     VIGAMLRTSYFQTPGGKAREYISFKFDSSKVPDLPKPRPYREIFVYAPRVEAVHLRFG
                     PVARGGLRWSDRREDFRTEVLGLVKAQMVKNTVIVPVGSKGGFFVKRSPVGGDRDAVL
                     AEGIACYRLFMNGMLDITDNLVDGKVVHPQNCVRHDGDDPYLVVAADKGTATFSDIAN
                     AISGEHGFWLGDAFASGGSVGYDHKAMGITAKGGWESVKRHFRALGRDCQSEDFTCVG
                     IGDMSGDVFGNGMLLSRHTRLLAAFDHRHIFLDPNPDAAKSFAERERMFKLPRSSWAD
                     YDAGQISAGGGIYPLSAKSIPISAPVREALGIDAGVDSLSPNDLKRAILKAPVDLLWN
                     GGIGTYVKAETESSADVGDRANNVIRINGKELRCKLVGEGGNLGCTQRGRIEAALNGV
                     LLNTDFIDNSAGVDTSDHEVNIKILLGDAVQRGELTYDTRNTQLAAMTDEVARLVLLD
                     NYRQNQAISLMERMSVARIGAKQHFIRTLESQGLLDRQIEFLPSDAEFAERKARGLGL
                     TRPELAILLSYSKIVLFQQLLESDVPEDAHLSKELRRYFPTPLQEKYAPHMERHRLKR
                     EIIATAVTNSMVNRMGATFVLRMGEDTGKTPAQVARAYNIAREVFDARDLWAGIEALD
                     GKVNGNTQIDALLVIWNALRGSTRWILNHSDATLDIAAAVDRYHQGMRDLRGHLVEAI
                     TPREKQRYDADKQRWLDAGFPDGLAADLAVLPTPGSGLDIIHVAADHGLQVERAAEVY
                     FALGEALYLSWLMDKIENLPVESRWHAHARGSLRDELYSQHRALTVQIIEHGGKGNGA
                     ELVEAWLNRDDPALKFTLGMFADMRSQLVADYPIVSVAVRRLAQLVQAGG"
     gene            3783..4544
                     /locus_tag="VFF05_03260"
     CDS             3783..4544
                     /locus_tag="VFF05_03260"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011097790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HZW50852.1"
                     /translation="MTAIAFAASKTDEAQAALADLHARYGQTPVEQADVIVALGGDGF
                     MLRTLHRYLALNKPVFGMKLGTVGFLMNQFRPEELPERLARAQSTPLRPLVMEAVSES
                     GTTTTSLAFNEVSLLRQTKQAAHIRVSLNAAVKIDELMCDGVLVCTSAGSTAYNLSAH
                     GPILPLGVDVLAMTPISPFRPRRWRGAILHAGTEVKFEILDPYKRPVSATADSNEVRD
                     VVEVTIRESREKTVTLLFDPEHNLEDRILNEQFAY"
     gene            4591..>5479
                     /locus_tag="VFF05_03265"
     CDS             4591..>5479
                     /locus_tag="VFF05_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZW50853.1"
                     /translation="MQKFVQGRVRLGVAIALACGSTAAAEVDPALLAQAQRVGSADTL
                     IVLDAAVPTALLRRDGDYLARRRNLVDTLRATADVSQESLRHWLDDNGVAYRSFWIVN
                     MIQAELTSAQIGDLAARDDVARLVENKAQPLRMPAPSTMPMQPEAVESVEWGVSKIRA
                     PQVWALGITGQGVTVAGQDTGIRWTHNSLKPHYRGWNGSVADHNYNWHDSIHDSTGNP
                     CGNDSLVPCDDDGHGSHTVGTMVGDDGAGNQVGVAPGAKWIGCRNMDAGNGTPARYNE
                     CAQWLLAPTDLAGSNIDPAK"
BASE COUNT          983 a         1774 c         1790 g          932 t
ORIGIN      
        1 gcgtgctcgg cctgcagggc cgcgcgcgcg cgcgcctgtt cgtgcgccgt gacaagttcg
       61 gtcgcttctt ctcttgcatg gcgttcgtgc cgcgtgaccg cttcaccgcc gacgtgcgcg
      121 agagaatcga gggtctgctc aaggacacgt tccacggcga gcgtctggat tccacgatca
      181 atgtcggcga atccgcgctg gcgcgcctgc atctgatcgt gcgtccgaag cccggcgaaa
      241 agcccgagta cgacaccgcg caactggaag aacgcatcgg ccagatcgtg cgcaactggc
      301 gcgacagcct gcgcgatgaa ttggtgcgca agcacggcga ggaaaagggt agcaagctgg
      361 ccagccgttt cggccgcgcg ctgcctgccg gctacatcga agaagtcagc gccgaggttg
      421 cggcggcgga cgtcgaatcc atcgccagcc tcaaggatgc cgacgaccta cgcctgtcgc
      481 tgtaccgacc cggcaaggac gacagcttgc gcttcaagct gttccgctac ggcgcgccga
      541 tttccttgtc ggacgcgttg cccctgctcg agaacatggg cctgaagatc ctttccgagc
      601 atccctacga aatgaagctc ggcgacagcg cggcggtgat ccaggaattc gaagcgcaga
      661 cggcggtgag ttgcggtttc gacatcgagc agatcaggga ttcgttccag gcgagcttcg
      721 aacgcatctg gcgcggccag gccgagaacg acggcttcaa caaactcatc ctcgcggcga
      781 acatgggctg gcgccaggtc gcgatgctgc gcggcctgtg caagtacatc ctgcagaccg
      841 gcgcgccgtt ctcgcaaagc tacatggaag agacgctcaa ccgctatccg ttgatcgccg
      901 gcctgctcgt ggaactgttc gaggccaggt tcgatccgca gcgcgaggat ccgggcaaga
      961 ccgtgctcga aggcgcgcgc aagcgcttgc gcaaggagct cgaaatcctg ttgccggcgg
     1021 aagtcgccaa ggcgcatcca gccttcgtcg atgatctgtt gaacgcgcgc gggcaaacgc
     1081 gcgatgcgca gatgcaggcg atcgtcgccg cgttgcgcat cctgctcgac cgcgtggcga
     1141 gtctggacga agaccgcatc ctgcgcggct tccttggtgt catcggcgcc atgctgcgca
     1201 cgagctattt ccagacgccc ggcggcaagg cgcgcgagta catcagcttc aagttcgatt
     1261 cgtccaaggt gccggacctg cccaagccca ggccctaccg cgagattttc gtctacgccc
     1321 cgcgcgtgga ggccgtgcat ttgcgcttcg gtccggtcgc gcgcgggggc ttgcgctggt
     1381 ccgatcgtcg cgaagacttc cgcaccgagg tgctgggcct ggtcaaggcg cagatggtca
     1441 agaacacggt catcgtgccg gtcggttcca agggcgggtt cttcgtcaag cgctcgccgg
     1501 taggcggcga tcgcgatgcg gttctcgccg agggcatcgc ctgctatcgc ctgttcatga
     1561 acggcatgct cgacatcacc gacaacctgg tcgacggcaa ggtggtgcat ccgcagaact
     1621 gcgtgcgcca cgatggcgac gatccctatc tcgtcgtcgc cgcggacaag ggcacggcga
     1681 cgttctcgga catcgccaac gcgatttccg gtgaacacgg tttctggctc ggcgacgcgt
     1741 tcgcctcggg cggctcggtc ggctacgacc acaaggcgat ggggatcacc gccaagggcg
     1801 gttgggaatc ggtcaagcgc catttccgcg cactcggccg tgattgccag tccgaggatt
     1861 tcacctgcgt cggcatcggc gacatgtccg gcgacgtgtt cggcaacggc atgctgctgt
     1921 cgcggcatac gcgcctgctc gccgcattcg atcaccgcca cattttcctc gatccgaatc
     1981 ccgatgcagc aaaatccttt gccgaacgcg agcgcatgtt caagttgccg cgctcgtcct
     2041 gggccgacta cgacgccggg cagatcagcg ccggcggcgg catttatccg ctcagcgcga
     2101 aatcgatccc gatttcggcg ccagtgcgcg aggcgctcgg catcgacgcc ggcgtcgatt
     2161 cgctgtcgcc gaacgatctc aagcgcgcga tcctcaaggc gccggtcgac ctgctctgga
     2221 acggcggcat cggcacctac gtcaaggccg aaaccgaatc cagcgccgat gtcggcgacc
     2281 gcgccaacaa tgtgatccgc atcaacggca aggaactgcg ttgcaagctg gtgggcgagg
     2341 gcggcaacct cggctgcacc cagcgcggac gcatcgaggc ggcgctcaac ggcgtactgc
     2401 tcaacaccga cttcatcgac aattcggcgg gcgtggacac atccgaccac gaggtcaaca
     2461 tcaagatcct gctcggcgac gccgtgcagc gcggcgagct gacctacgac acgcgcaaca
     2521 cgcagctcgc ggcgatgacc gacgaggtcg cgcgcctggt gctgctcgac aactaccgcc
     2581 agaaccaggc gatcagcctg atggaacgca tgtcggtggc gcgcatcggc gccaagcagc
     2641 atttcatccg caccctggaa agccagggcc tgctcgaccg ccagatcgag ttcctgccca
     2701 gcgacgccga gttcgccgag cgcaaggcgc gcgggttggg cctgacgcgc ccggaactgg
     2761 cgatcctgct gtcgtattcc aagatcgtgc tgttccagca actgcttgaa tcggacgtgc
     2821 cggaagatgc gcatctgtcc aaggaattgc gccgctactt tcccacgccg ctgcaggaaa
     2881 aatacgcgcc gcacatggag cgccaccgcc tcaagcgtga gatcatcgcc accgcggtga
     2941 ccaactcgat ggtcaaccgc atgggcgcca cgttcgtgct gcgcatgggt gaagacaccg
     3001 gcaagacccc ggcgcaggtg gcgcgcgcct acaacatcgc ccgcgaagtg ttcgatgcgc
     3061 gcgatctgtg ggccggcatc gaggcgctgg acggcaaggt caacggcaac acgcagatcg
     3121 acgccttgct tgtgatctgg aacgcgctgc gcggttccac gcgctggatc ctcaatcatt
     3181 ccgacgcgac gctggacatc gccgccgcgg tggatcgcta ccatcagggc atgcgcgacc
     3241 tgcgcgggca tctggtcgag gcgatcacgc cacgcgagaa gcagcgctat gacgccgaca
     3301 agcagcgctg gctggatgcc gggttcccgg acggtcttgc ggcggatctc gccgtgctgc
     3361 cgacgccggg ttcgggcctg gacatcatcc acgtcgccgc cgatcacggc ttgcaggtcg
     3421 aacgcgccgc ggaagtctat ttcgcgctcg gcgaggcgct gtatctgtcg tggctcatgg
     3481 acaagatcga gaacctgccg gtcgaatcgc gctggcacgc gcacgcacgc ggcagcctgc
     3541 gcgacgagct ctacagccag caccgcgcgc tgactgtgca gattatcgag catggcggca
     3601 aaggcaatgg cgcggaactg gtcgaggcat ggctcaaccg cgacgacccg gcgctgaagt
     3661 tcaccctggg catgtttgcc gacatgcgca gtcagctcgt cgccgattat ccgatcgtct
     3721 cggtcgccgt gcgtcggctt gcgcaactgg tgcaggcggg cgggtgaggc cgacgtgacc
     3781 acatgactgc catcgccttc gccgccagca agaccgacga agcgcaagcc gcgctggcgg
     3841 acctgcacgc gcgttatggc cagacgccgg tggagcaggc cgacgtgatt gtcgctcttg
     3901 gcggcgacgg tttcatgctg cgcacgctgc accgctatct cgccctgaac aagccggtgt
     3961 tcggcatgaa gctcggcacg gtcggcttcc tgatgaacca gttccgtccc gaggaattgc
     4021 ccgagcgcct ggcgcgcgcg cagtccacgc cgctgcgccc gctggtgatg gaggcggtga
     4081 gcgaatccgg gacgacgacc acgtcgctgg cgttcaacga agtttcgctg ctgcgccaga
     4141 ccaagcaggc cgcgcacatc cgcgtctcgc tcaacgccgc ggtgaagatc gacgagttga
     4201 tgtgcgacgg cgtgctggtg tgtacgtcgg ccggttccac ggcctacaac ctttccgcgc
     4261 acgggccgat cctgccgctg ggcgtggatg tgctggcgat gacgccgatc agtccgttcc
     4321 ggccgcggcg ctggcgcggt gcgatcctgc acgccggcac cgaagtgaaa ttcgagattc
     4381 tcgatcccta caagcgtccg gtcagcgcca cagccgattc gaacgaagtg cgcgacgtgg
     4441 tcgaggtgac gatccgcgag tcgcgcgaaa aaaccgtgac cttgctgttc gatccggaac
     4501 acaatctcga agatcggatt ctgaatgagc agtttgcgta ttgagcgata gactttctgt
     4561 tcacgttccg attcgttctg gggagatacg atgcaaaagt tcgttcaagg ccgagttcgg
     4621 ttgggcgttg cgattgcgct cgcctgcggt tcgacggcgg ccgcggaagt cgatccggcg
     4681 ctgcttgcgc aggcgcagcg agtcggcagc gcggacacgc tgatcgttct tgatgccgcg
     4741 gtgccgactg cgctgctgcg tcgggatggc gactacctcg cgcggcgccg caatctcgtc
     4801 gataccttgc gcgccaccgc cgatgtgtcg caggagtcgt tgcggcactg gctggacgac
     4861 aacggcgtgg cctatcgcag tttctggatc gtcaacatga tccaggccga gctgacgtcg
     4921 gcgcagatcg gcgatctggc cgcgcgcgac gacgtggcac ggctggtgga aaacaaggcg
     4981 cagccgttgc gcatgcccgc gccatcgacg atgccgatgc agcccgaggc ggtcgaatcg
     5041 gtggagtggg gtgtgagcaa gatccgcgcg ccgcaggtgt gggccctggg catcaccggt
     5101 cagggtgtga ccgtcgccgg ccaggacacc ggcatccgct ggactcacaa ttcactaaag
     5161 ccgcattatc gtggctggaa cggcagcgtc gccgaccaca actacaactg gcacgactcg
     5221 atccacgatt ccaccggcaa tccctgcggc aacgattccc tcgttccctg cgacgatgac
     5281 ggccacggct cgcacacggt cggcacgatg gtgggcgacg atggcgccgg caaccaggtc
     5341 ggcgtcgcgc cgggcgcgaa atggattgga tgccgcaaca tggatgcggg caacggtacg
     5401 ccggcccgct acaacgaatg cgcacaatgg ctgctggcgc ccaccgatct ggccggtagc
     5461 aatattgacc cagccaagg
//
LOCUS       DASRVO010000265         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_8181, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000265 DASRVO010000000
VERSION     DASRVO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8181"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFF05_03270"
     CDS             complement(<1..262)
                     /locus_tag="VFF05_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HZW50854.1"
                     /translation="MRTIALALVFALASSATNAAEALPLDELHLPPGFHIAQFARVPN
                     AREMTLGANGTVFVGSMSAGKVYALTSSTDGTHADKVRIVAQG"
     gene            complement(262..1257)
                     /locus_tag="VFF05_03275"
     CDS             complement(262..1257)
                     /locus_tag="VFF05_03275"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW50855.1"
                     /translation="MTDLQQALNLHRAGDLDAAEAAYRALLADRPDDADALHLLGTLL
                     HKRGHAVAAEESIRNAMMLDPECAQFHLSLGGVLMQQGRDAEAHAAFERALELDPNAP
                     EAHALLGFVHARAGDMAAAEERFKIGRRAEADDPLLLLGLGNLYLTREQPDNALKFLT
                     RAAEQRPDDAAIQFSLGRAFFEQGNFAFADKAFENALRVQPDLDRARLYRGRVCVREK
                     RYADAQAIYEDLLARKQQEFGALAGLGDCARHRGHVLRALKFYRRALALEPDNPGAAN
                     ACAWCLEQVGDLAGAAEYLSGALQRAPDAAWLRTPLAGLLERLGRGEEAAQVRTG"
     gene            1374..>2192
                     /locus_tag="VFF05_03280"
     CDS             1374..>2192
                     /locus_tag="VFF05_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50856.1"
                     /translation="MRSASWVSGLCLLAGMACAQAAPLLLQTPSVSPSHIAFAYGGEI
                     WTVPRAGGAATLLVGGEGTAMDPVFSPDGAWVAYTDTANGNADVFVVATAGGTPKRLT
                     WHPAGDEAVGWSPDGKSVLIRSNRDAANDSSQLYLLSLSGGLPQALPLSMAEQGAFSP
                     DGKQIAYNPVFQWEPDWRDYRGGQTQRIWIARLSDSSVTKVPRENSNDSDPMWLGSRV
                     YFLSDRNGPATLFAYDVAGGKVDQIVANDGYPIDAASAGAGAIVYTQMGELHLVD"
BASE COUNT          360 a          713 c          740 g          379 t
ORIGIN      
        1 gaccttgcgc gacgatgcgc accttgtccg cgtgcgtgcc gtcggtcgac gacgtgagag
       61 cgtagacctt gcctgcactc atcgaaccga cgaacaccgt gccgttcgcg ccgagcgtca
      121 tctcgcgcgc attcggcacg cgtgcgaatt gcgcgatgtg gaacccgggc gggagatgca
      181 actcgtccag cggcaacgcc tccgcggcgt tggtcgccga cgaggccagc gcgaaaacca
      241 gcgcgagcgc aattgtgcgc atcaaccggt ccgtacctgc gcggcttcct cgccacgacc
      301 gaggcgttcg agtaaaccgg caagcggcgt gcgcagccac gccgcatccg gtgcgcgttg
      361 cagggcgccg gacagatatt ccgccgcacc ggccaggtcg ccgacctgtt caaggcacca
      421 ggcgcaggca ttggccgcgc cgggattgtc cggttccagc gccagcgcac gtcgatagaa
      481 cttcagcgcg cgcaggacat ggccgcgatg gcgcgcgcag tcgccgagtc cggccaacgc
      541 accgaattcc tgctgcttgc gggcgagcag atcctcgtag atcgcctgtg catccgcgta
      601 gcgcttctcg cgcacacaga cgcggccgcg atacaggcgt gcgcgatcca ggtccggctg
      661 cacgcgcagc gcattctcga acgccttgtc cgcgaacgcg aaattgccct gttcgaagaa
      721 ggcacggcca agactgaact ggatcgccgc gtcgtcgggc ctttgctccg ctgcgcgggt
      781 cagaaacttg agcgcgttgt ccggctgttc gcgcgtcagg tataaattgc ccaagccgag
      841 cagcaacagc ggatcgtccg cttcggcacg ccggccgatc ttgaagcgct cttcggcggc
      901 ggccatgtcg cccgcgcgtg cgtgcacgaa gccgagcagc gcgtgcgcct ccggcgcgtt
      961 cggatcgagt tcgagagcac gttcgaacgc ggcgtgcgcc tccgcgtcgc gaccctgctg
     1021 catcagcacg ccgcccagcg acaaatgaaa ctgcgcgcat tcgggatcga gcatcatcgc
     1081 gttgcggatc gattcctccg cagccacggc atggccgcgc ttgtgcaaca gcgtaccgag
     1141 cagatgtagc gcgtccgcgt cgtcgggacg gtcggccagc agcgcgcggt acgccgcctc
     1201 ggcggcatcg agatcgccgg cgcggtgaag attcaaggct tgttggagat cggtcacggg
     1261 aaggtggcga gtggatcaga ccgctattat gccgcagggt gttaccaatg gcaggcttga
     1321 tccggacagg cgcggcctag actcgcgctt tgccgtcgga ttcggagagt ttcatgcgtt
     1381 cagcatcatg ggtatcgggc ttgtgccttt tggccggaat ggcttgcgcg caggccgcgc
     1441 cgttgctgtt gcagacgccg agcgtatcgc cttcgcacat cgccttcgcc tacggcggcg
     1501 aaatctggac ggtaccgcgc gccggcggtg cggcgacgct gctggtcggc ggcgagggca
     1561 cggcgatgga tccggtgttt tcgccggacg gcgcctgggt cgcctacacc gataccgcga
     1621 acggcaatgc cgacgtgttc gtggtcgcga ccgcgggcgg cacgccaaag cgcttgacct
     1681 ggcacccggc tggagacgaa gccgtgggct ggtcgccgga cggcaagtcc gtgctgatcc
     1741 gctccaatcg cgatgcggca aacgattcct cgcagttgta cctgctgtcg ttgtccggcg
     1801 gcctgccgca ggcattgccg ctgtccatgg ccgagcaagg cgcgttttcg ccggacggca
     1861 agcagatcgc ctacaacccg gtgttccagt gggagccgga ttggcgcgac taccgcggcg
     1921 gccagaccca gcgcatctgg atcgcgcgac tgtcggattc gagcgtgacg aaggttccgc
     1981 gcgagaattc caacgactcc gatccgatgt ggctcggcag ccgcgtctac tttttgtccg
     2041 accgcaatgg tccggcaacg ctgtttgcct atgacgttgc gggcggcaag gtggatcaaa
     2101 tcgtcgccaa tgacggttat ccgatcgacg cggcgagcgc cggtgcgggt gccatcgtct
     2161 acacgcagat gggcgagctg catctcgtgg at
//
LOCUS       DASRVO010000266         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_137648, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000266 DASRVO010000000
VERSION     DASRVO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137648"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFF05_03285"
     CDS             complement(<1..211)
                     /locus_tag="VFF05_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZW50857.1"
                     /translation="MAFVQAPPVLSNQFLDDRVLRSYLRRVLPPATLAAIEGDLTDLG
                     EYAVNAWTQARGRQASEPRLTHWNAW"
     gene            complement(332..673)
                     /locus_tag="VFF05_03290"
     CDS             complement(332..673)
                     /locus_tag="VFF05_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HZW50858.1"
                     /translation="MSGSPRALRLGAIALLGQLVLQLLWNAWLAPATRAALALSVLPL
                     LPGLWICLGNLRRGVLVGGIVSLFYFCHGVANLWSDPGSRLPSAAEVALSLAVIAASW
                     WDARGYKRPPK"
     gene            complement(670..>1024)
                     /locus_tag="VFF05_03295"
     CDS             complement(670..>1024)
                     /locus_tag="VFF05_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZW50859.1"
                     /translation="GNMAAPLKHFLDTTGAEWVSGALAGKPAAVFTSTSTMHGGQETT
                     LISMMLPLLHHGMLLVGLPFTEAAINSTTTGGTPYGASHVAGIDGSRPLAEDERTLAR
                     ALGKRVADLAVRLAR"
BASE COUNT          182 a          333 c          361 g          148 t
ORIGIN      
        1 cccaggcgtt ccagtgggtc aggcgcggct cgctcgcttg gcgcccgcgc gcctgcgtcc
       61 aggcgttgac cgcgtattcg ccgagatcgg tcaggtcgcc ctcgatcgca gccagggttg
      121 ccggcggcag cacccggcgc agatatgaac gcaggacacg gtcatcgaga aactggttgc
      181 tcagtaccgg cggagcctgg acaaaagcca tctgtattcc cttggcggca ggcaggagaa
      241 aactacacgg cggcgggacg gtcgacaagg cgcgaacaat actgtagcgc gccaggcgtc
      301 cggcgcgttg tgaacggatc ggcacggccg gctatttcgg cggacgtttg tagccgcgcg
      361 catcccacca ggatgcggcg atgaccgcca ggctcagcgc gacctctgcc gccgacggca
      421 ggcgcgatcc cggatcgctc cacaggttgg cgacgccgtg gcagaaatag aacagactga
      481 cgatgccgcc gacgaggacg ccgcggcgca ggttgccgag gcagatccac aggcccggaa
      541 gcaacggcag caccgacagc gcaagcgcag cacgcgttgc gggcgcgagc caggcgttcc
      601 agagcagttg cagtaccagc tggccgagca gcgcgattgc gcccagtcgc aaggctcgcg
      661 gcgatccgct catcgggcga gccgcaccgc gagatcggct acacgcttgc cgagcgcgcg
      721 tgccagggtg cgctcgtcct cagccagtgg acggctgccg tcgattccgg cgacgtggct
      781 agcgccatag ggcgtgccgc cggtcgtggt cgagttgatc gcggcttcgg tgaacggcaa
      841 tccgaccagc agcatgccat ggtgcagcaa cggcagcatc atcgagatca gcgtcgtctc
      901 ctggccgccg tgcatcgtcg aggtcgaagt gaatacggcc gcgggcttgc cggccagagc
      961 gccggagacc cattcggctc cggtcgtgtc gagaaagtgc ttgagcggcg cggccatgtt
     1021 gccg
//
LOCUS       DASRVO010000267         1060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_8198, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000267 DASRVO010000000
VERSION     DASRVO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8198"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /gene="cysC"
                     /locus_tag="VFF05_03300"
     CDS             <1..764
                     /gene="cysC"
                     /locus_tag="VFF05_03300"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HZW50860.1"
                     /translation="ETQPATRLEMNEVGYVHLSLDHAVAFEPYAKNRALGGFVLIDRQ
                     SLATAACGTLDFALRRAANIHWQALSLDRAARAASLQQHPRCLWFTGLSGAGKSTIAN
                     QVEQRLHALGRHTYVLDGDNVRHGLNRDLGFTDEDRVENIRRVAEVARLMVDAGLIVL
                     VSFISPFRDERRMARDLFGPGDFAEVYVDTPIELCEKRDPKGLYAKARAGELQNFTGV
                     DSPYEPPLQPELHLRTEKESVDALARRIVEFLERR"
     gene            complement(778..>1060)
                     /locus_tag="VFF05_03305"
     CDS             complement(778..>1060)
                     /locus_tag="VFF05_03305"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645449.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HZW50861.1"
                     /translation="TLRISSQHIANWLLHGIVSREQVKQTLQRMAAVVDAQNTATPDY
                     EPMAGNVDGACAFQAARDLIFTGVEQPNGYTEPVLHRWRRKKKAQQAGR"
BASE COUNT          173 a          354 c          349 g          184 t
ORIGIN      
        1 aggaaacgca gccggcgacg cgactggaaa tgaacgaggt cggctacgtg cacctgagcc
       61 tggaccacgc cgtcgcgttc gagccgtatg cgaagaaccg cgcgctcggc ggcttcgtgc
      121 tgatcgaccg ccagagcctg gccaccgccg cctgcggcac gctggatttc gccctgcgcc
      181 gcgccgccaa catccattgg caggcgctga gcctggatcg cgccgcgcgc gcggccagcc
      241 tgcagcagca tccgcgctgc ttgtggttca cgggcctgtc cggcgccggc aagtcgacca
      301 tcgccaacca ggtcgagcag cgcctgcacg cactcggtcg ccacacctat gtgctcgatg
      361 gcgacaacgt gcgccatggc ctgaatcgcg acctcggctt taccgacgag gatcgcgtcg
      421 agaatatccg acgcgtcgcc gaagtcgcgc gattgatggt cgatgcggga ctcatcgtgc
      481 tggtcagctt catctcgccg ttccgcgacg agcggcgcat ggcgcgcgac ctgttcggcc
      541 ccggcgactt cgccgaggtc tacgtcgata cgccgatcga actgtgcgag aagcgcgatc
      601 cgaaaggcct ttacgccaag gcgcgcgccg gcgaactgca aaacttcacc ggtgtggatt
      661 cgccctacga gccgccgttg caaccagaat tgcacctgcg caccgaaaaa gagtcggtgg
      721 acgcgctcgc gcgccggatc gtggagttcc tcgaacgcag gtagcgcctc ggtagcgtca
      781 gcgcccggct tgctgcgcct tcttcttgcg ccgccagcgg tgcagcaccg gctcggtgta
      841 accgttcggt tgctcgacgc cggtgaagat caggtcgcgg gcggcctgga atgcgcaggc
      901 gccatcgacg tttccagcca tcggttcgta gtccggcgtg gcggtgttct gcgcatcgac
      961 cacggcggcc atgcgctgca gcgtttgctt gacctgttcg cgactgacga tgccgtgcag
     1021 tagccagttg gcgatgtgct ggctggaaat gcgcaaggtc
//
LOCUS       DASRVO010000268         1308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_60048, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000268 DASRVO010000000
VERSION     DASRVO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60048"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1308)
                     /locus_tag="VFF05_03310"
     CDS             complement(<1..>1308)
                     /locus_tag="VFF05_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HZW50862.1"
                     /translation="TLLGTIDAPGGFRHKAPYPRHIVPNHRAFNAPDQIQPNTPLNAA
                     PLGFPASPEELAILPDGTPMRIDHAFSWEYPLAAHGLMHNVLANAVRGDPYRIDTLLL
                     FMANMAWNSSMDPLTSRELLTRKDGDGQYMIPFLVVCDTFQSETVAFADLVLPDTTYL
                     ERHDVMSMLDRPISEFDGPVDSVRVPVVAPPAGCKPFQEVLVELASRLKLPAFTRADG
                     ARKFGGYEDFIVNFEAQPGVGFLMGWRGADGSEPLRGAPNPKQWEMYAANNCVFQYHL
                     PQDMQYMRNWNRAYLDFAQAKGWRQKNDPIQLAIYSDCLQSFRLAAQGRTSGRQPPQH
                     LRQRVATYFDPLPFWYPPLEDGVTDRGAYPLNAVTQRPMAMYHSWDSQNAWLRQIHAH
                     NLLYVNAQTAKIAGIADNDWIWVESQWGKVRCMAKHSEAVEPG"
BASE COUNT          206 a          425 c          446 g          231 t
ORIGIN      
        1 tgccgggctc gaccgcctcg gagtgcttgg ccatgcagcg caccttgccc cattggctct
       61 cgacccagat ccagtcgttg tcggcaatac cggcgatctt cgccgtctgg gcgttcacgt
      121 acagcaggtt gtgcgcatgg atctgccgca gccaggcgtt ctgcgaatcc cacgagtgat
      181 acatcgccat cgggcgctgc gtgaccgcat tgagcggata cgcgccgcgg tcggtcacgc
      241 cgtcttccag cggcggatac cagaacggca gcggatcgaa gtaggtcgcg acgcgctggc
      301 gcagatgctg cggcggttgc cgcccgctcg tcctgccttg cgctgcgagc cggaagcttt
      361 gcaggcaatc ggagtagatc gccagctgga tcggatcgtt cttctggcgc cagcccttgg
      421 cttgcgcgaa atccaggtag gcacggttcc agttgcgcat gtactgcatg tcctgcggca
      481 ggtggtactg gaacacgcag ttgttggccg cgtacatctc ccactgcttc gggttcggcg
      541 cgccgcgcag cggctcgctg ccgtcggcgc cgcgccatcc catgagaaag ccgaccccgg
      601 gctgcgcctc gaaattgacg atgaagtcct cgtagccgcc gaacttgcgc gcgccgtcgg
      661 cgcgcgtgaa cgccggcaac ttcaggcgcg atgccagctc gaccagcact tcctggaacg
      721 gcttgcagcc ggccggcggc gcgaccacgg gcacgcgcac ggaatcgacg ggcccgtcga
      781 attcggagat cggccggtcg agcatgctca tgacgtcgtg ccgttcgaga tacgtcgtgt
      841 ccggcagcac gagatcggca aacgcgaccg tctcgctctg gaacgtgtcg cacaccacca
      901 ggaacgggat catgtattgc ccgtcgccgt ccttgcgcgt gagcaactcg cgcgaggtca
      961 gcgggtccat gctggagttc cacgccatgt tggccatgaa cagcagcagc gtgtcgatgc
     1021 ggtacgggtc gccgcgcacg gcgttggcca gcacgttgtg catcaggccg tgcgcggcca
     1081 gcgggtactc ccacgagaac gcatggtcga tgcgcatcgg cgttccgtcg ggcaggatcg
     1141 cgagctcctc ggggctggcc ggaaacccca gcggcgcggc gttgagcggc gtgttcggct
     1201 ggatctgatc gggcgcgttg aacgcacggt gattgggcac gatgtggcgc ggatacggcg
     1261 ccttgtgccg gaagccgccg ggcgcatcga tcgtgcccag cagcgtca
//
LOCUS       DASRVO010000269         1323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_86154, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000269 DASRVO010000000
VERSION     DASRVO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86154"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /locus_tag="VFF05_03315"
     CDS             <1..1297
                     /locus_tag="VFF05_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW50863.1"
                     /translation="FPLAEMLIEAGRGAAAVEVVRDCFANGAHDAELAIRAIELLDEA
                     GRKRAAADIAETALAAQPTDARLHAYAGMLDVQLGEFARAREHYLFALAHDARACEWN
                     APHGLSSAQRYADAEHPDFDLFRDCLARTDLSTKARAALLFAVGKAHDDIGDYAQAAD
                     FFRQANALAHEATAWSRKNWRRAIESRLASKPFAQHLHAPDFVPVFIVGMPRSGTTLA
                     AELLARRLGVRNRGELPWLAKLAQLPVLAGNPSHAVLARCAEIYAAQLRQDDSGDARF
                     FIDKQPLNFRYIDLALALFPNARIVHCTRGARDTALSLWVQSFSEDVQGYACDFADIA
                     LVMRDEERLMAHWSERYPEAIRALRYEDVAADPGQQIGELSQWLGLPTNTASSTDTAP
                     EAIGTASLWQARQPVYTRSIGRWRHYAPYVSELEKFAGG"
BASE COUNT          228 a          416 c          443 g          236 t
ORIGIN      
        1 gtttccactg gcggaaatgc tgatcgaagc cggtcgtggc gcggctgcag tcgaagtcgt
       61 acgggactgt tttgcgaatg gcgcgcacga tgcggaactc gcaatccgcg ccatcgaact
      121 gctcgacgag gccgggcgca agcgggccgc ggcagacatt gccgaaaccg cgctcgcggc
      181 gcagccgacg gatgcgcgtc tgcacgccta cgccggcatg ctggacgtgc aactgggtga
      241 gttcgcacgc gcgcgcgagc actacttgtt tgcactagcg cacgatgcgc gggcctgcga
      301 atggaacgcg ccgcacggat tgtccagcgc acaacgctac gcggatgcgg agcatccgga
      361 tttcgacctc tttcgcgact gcctggcgcg caccgacctt tcgaccaagg cccgcgccgc
      421 gctgctgttc gccgtcggca aggctcacga cgacatcggc gactatgcgc aagcagcgga
      481 tttctttcgg caagccaacg cgcttgcgca tgaggcgacg gcatggtcgc gcaagaactg
      541 gcggcgcgcg atcgagtctc ggctggcatc gaaaccgttc gcgcagcatt tgcacgcgcc
      601 ggatttcgtc ccggtgttca tcgtcggcat gccgcgctcg ggaacgacgc tggccgccga
      661 gttgctcgcg cgtcggctcg gcgtgcgcaa tcgcggcgaa ttgccatggt tggccaaact
      721 cgcgcaactg cccgtgctgg cgggcaaccc gtcgcatgcc gtgctcgcgc gctgcgcgga
      781 gatctacgcg gcgcagctgc gccaggacga ttccggtgac gcgcgctttt tcatcgacaa
      841 gcagccgctg aatttccgct acatcgattt ggcgctggcg ttgtttccga acgcgcgcat
      901 cgtgcactgc acgcgtggcg cacgcgacac tgcgttgtcg ctgtgggtgc aatctttcag
      961 cgaggacgtg cagggctacg cctgcgattt cgccgacatc gccttggtga tgcgcgatga
     1021 agagcgtctg atggcgcatt ggagcgaacg ctatccggaa gcgatccgcg cgctgcgtta
     1081 cgaagacgtc gcagcggatc cgggacagca gatcggcgag ctttcacaat ggcttggttt
     1141 gccgacgaat acggcgtcat cgacggatac cgcacccgaa gcgatcggca cggcaagttt
     1201 gtggcaggcg cgccagccgg tctacacgcg ttcgatcggg cgctggcgcc attacgcgcc
     1261 ctacgtgtcg gaactggaaa agttcgccgg gggatgaacc gctcaagccg gatcggtgga
     1321 ata
//
LOCUS       DASRVO010000270         1123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_216076, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000270 DASRVO010000000
VERSION     DASRVO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216076"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VFF05_03320"
     CDS             complement(<1..163)
                     /locus_tag="VFF05_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZW50864.1"
                     /translation="MTDSRFSGIDRLYGAGSAARLARAHVAVVGLGGVGSWAAEALAR
                     SGVGTLTLID"
     gene            239..619
                     /locus_tag="VFF05_03325"
     CDS             239..619
                     /locus_tag="VFF05_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50865.1"
                     /translation="MRQAILFVLGLAVGAIAAANIVSTLRQRDAYPRGLMNVMQHDTG
                     VLHDAARADRCDAATQGALERLHGLAGEIETAVYGAEPPDPPFAEYAQRLRTAIPSSL
                     ECKSLPQALDRVGAACDDCHRQYR"
     gene            complement(651..>1123)
                     /locus_tag="VFF05_03330"
     CDS             complement(651..>1123)
                     /locus_tag="VFF05_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HZW50866.1"
                     /translation="PEFHHHAAESLPAWENNGVRLRLVAGEAFGYRSPVHTFSRLFYV
                     AVEFPVGGSLVVPAEHIERAVYATDAPLSVGGVELAVAHMAVLPAGMEVEIRATQPAR
                     VILCGGDPLDGDRHLWWNFVSSSRERIEKAKADWAAMRYPPVPGETEFIPLPQH"
BASE COUNT          199 a          395 c          337 g          192 t
ORIGIN      
        1 cgtcgatcag cgtcaatgtg ccaacgccgg aacgcgccag cgcctcggcc gcccacgagc
       61 cgacgccgcc cagcccgacc acggccacgt gcgcgcgcgc caggcgtgcc gcgctgccgg
      121 cgccgtaaag ccggtcgatt ccgctgaatc gcgagtccgt cattgcccgt ccatgttcac
      181 tgcgaactta ctttagcatc cggattcgct atcatttccg ttttcgtcgg agcggcgcat
      241 gcgtcaagcg atcctgttcg tgctcggctt ggcggtcggc gccatcgccg cggccaatat
      301 cgtcagcacg ctgcgtcagc gcgacgccta tccgcgcggc ctgatgaatg tcatgcagca
      361 cgataccggc gtcctgcacg atgccgcgcg cgcggaccgt tgcgatgccg caacccaagg
      421 cgccttggag cggttgcacg gtcttgctgg cgagatagaa acggcggtct acggcgccga
      481 gccgccggac ccgccgttcg ccgaatatgc acagcgcctg cgtacggcga ttccgtcctc
      541 gctggagtgc aagagtttgc cgcaggccct ggatcgcgtg ggcgcggcct gcgacgactg
      601 ccatcggcaa tatcgctgag tatttagcgc gcgttccctg ctcgcgcttc tcaatgttgc
      661 ggcagcggaa tgaactcggt ttcgcccgga acgggcggat agcgcatcgc cgcccaatcg
      721 gccttcgctt tctcgatgcg ttcgcgcgaa ctggatacga agttccacca cagatgacgg
      781 tcgccgtcga gcggatcgcc accgcacagg atgacccgcg ccggttgcgt cgcgcggatt
      841 tccacctcca tcccggccgg caagaccgcc atgtgcgcga ccgccaattc gactccaccg
      901 acactcagcg gcgcatccgt cgcgtacacc gcacgctcga tgtgttcggc gggaacgacc
      961 agcgatccac cgaccggaaa ttccacggca acgtaaaaca accgcgagaa cgtgtgcacc
     1021 ggcgaacgat agccgaaggc ttcgcccgcg accaaacgca gacgcacacc attgttttcc
     1081 cacgccggca acgattccgc cgcgtgatgg tgaaattcag gca
//
LOCUS       DASRVO010000271         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_320188, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000271 DASRVO010000000
VERSION     DASRVO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320188"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VFF05_03335"
     CDS             <1..719
                     /locus_tag="VFF05_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50867.1"
                     /translation="TDNEDQHIRTLYQSVGLRHMFNRDWGLSVDLPYDQRHFATLDDD
                     VPIITHHGQIGDIRITGMYTGFSGDMSSGLTFGLKLANGDWKVPGFDRDTQIGSGSTD
                     ALLGGYHQFRFGGLAGKWSGFAQALMDTPVDSQGGYRPGTELDAAFGAYPDSWRLAPG
                     VELTPLAQVVASLRRPDSGTAADADNTGFARLLAAPGLELRFKRLRVFADIEAPLWQN
                     VRGNQLIAPWQAKLTVSLRL"
     gene            complement(732..884)
                     /locus_tag="VFF05_03340"
     CDS             complement(732..884)
                     /locus_tag="VFF05_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50868.1"
                     /translation="MTLCPVALAVGCKRCPVFAICPAKSLIGDYRPESKKSAKPEAQA
                     VRKPKR"
     gene            complement(1046..>1733)
                     /locus_tag="VFF05_03345"
     CDS             complement(1046..>1733)
                     /locus_tag="VFF05_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HZW50869.1"
                     /translation="DAAIMDGATLRAGGVANVHRVKNPILLARSVMEHSPHVLRFGDG
                     AEEFAKTQGMALVDPKYFWTQQRWQELQDALKKDAAGQHADAEKEGHFGTVGAVALDK
                     DGHLAAGTSTGGMTDKRFGRVGDSPIIGAGTYANGGCAVSGTGWGEFYLRTVAAHEIC
                     MRVGEMHQPLQQAAEYVINTQIPKLGGDGGAIVLGADGEFATPFNTEGMFRGWVDKNG
                     KIHVAIFPGE"
BASE COUNT          279 a          580 c          535 g          339 t
ORIGIN      
        1 ccacggacaa cgaggaccag cacatccgta ccctgtacca gagcgtgggc ctgcgccaca
       61 tgttcaatcg cgattggggt ttgtccgtgg acttgcccta cgaccagcgc catttcgcga
      121 cactcgacga cgacgttccg atcatcaccc accacggtca gatcggcgat atccgcatca
      181 ccggcatgta caccggattt tccggcgaca tgtccagcgg gctcacgttc ggcctgaaac
      241 tggccaatgg cgactggaag gttccgggct tcgaccgcga cacccagatc ggcagcggca
      301 gtaccgacgc attactcggc ggttaccatc aattccgctt cggcggcctc gccggcaaat
      361 ggagcggctt cgcgcaggcg ctgatggata cgccggtgga ttcgcaaggc ggctatcgtc
      421 cgggtacgga actcgatgcc gccttcggcg cgtatccgga tagctggcgg ctcgcgccgg
      481 gcgtcgaatt gacgccgctg gcgcaggtcg tcgcctcgct gcgccgtccg gacagcggta
      541 cggcggccga tgcggacaac accggctttg cgcgcctgct tgccgcaccg ggtctggagt
      601 tgcgcttcaa gcgcctgcgc gttttcgccg acatcgaagc gccgctgtgg cagaacgtgc
      661 gcggcaacca gctcatcgcg ccgtggcagg caaaactcac cgtcagcttg cgcctgtagc
      721 ccctcgccgt ttcagcgttt gggtttgcgc acggcctgcg cttcgggctt cgcggatttt
      781 ttactttccg gccggtagtc gccgatcagc gatttcgccg gacagatcgc aaacactggg
      841 cagcgtttgc agccgacggc gagagcaacg gggcacaaag tcatggtgtt ctcctgttga
      901 cctctgaata acctgctgca ctcgacatct cgcgcgccgg cagtgctcga aatcctcaca
      961 tacggcaaag tatgctccga ttgctgcgcg ctggcggcgc acggcctgtc ttcgctcgcg
     1021 atggttcttc agaggcccct aaaattcatt cgccggggaa aatcgcgacg tggatttttc
     1081 cgttcttgtc cacccagcca cggaacatgc cctcggtgtt gaacggcgtg gcgaattcgc
     1141 catccgcgcc aagcacgatt gcgccgccgt cgccgccgag cttgggaatc tgcgtgttga
     1201 tgacgtactc ggccgcctgc tgcagcggct ggtgcatttc gccgacgcgc atgcagatct
     1261 cgtgcgcggc cacggtgcgc aggtagaact cgccccagcc ggtgccggat acggcacagc
     1321 cgccattcgc gtaggtgccc gcgccgatga tcggcgaatc gccgacgcgg ccgaagcgct
     1381 tgtcggtcat gccgccggtg gacgtgcccg cagcgaggtg tccgtccttg tccagcgcaa
     1441 ccgcaccgac cgtgccgaag tggccttcct tctcggcgtc ggcgtgctgg ccggcagcat
     1501 cctttttcag cgcgtcctgt agttcctgcc agcgctgttg cgtccagaag tatttcggat
     1561 cgaccagggc catgccctgc gtcttggcga attcctcggc gccgtctccg aacctcagca
     1621 cgtgcggcga atgttccatc acgctgcgcg caagcaggat gggattcttc acgcgatgca
     1681 cgttggccac gccgccggcg cgtagcgtcg cgccgtccat gatcgcggca tcc
//
LOCUS       DASRVO010000272         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_190673, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000272 DASRVO010000000
VERSION     DASRVO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190673"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFF05_03350"
     CDS             <1..244
                     /locus_tag="VFF05_03350"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HZW50870.1"
                     /translation="REFGELNKDFQRKQREFREDLNQRRNEELAAVLERANRTIKAIA
                     EAEKYDIIFQEAVYASPRIDITDKVIKALEDKPAGK"
     gene            254..>1020
                     /gene="lpxD"
                     /locus_tag="VFF05_03355"
     CDS             254..>1020
                     /gene="lpxD"
                     /locus_tag="VFF05_03355"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZW50871.1"
                     /translation="MAKAKKYRLAQIVERFGGEIVGNPQTPISLVATLQSAGAQDISF
                     LSHSKYRKQLAGTDAGAVILARADSDLTPLPRIVCADPYLYFAKVSSLFNPQPAARPG
                     VHRSAVVERGTKVPASASVGAGAYIGRGVKLGKGVAIGAGCYVGDAVEIGAGSRLHAR
                     VVIYAGCRLGERVQVHSGAVIGADGFGIAWNGEAWSKIPQIGGVLIGDDVEIGANTCI
                     DRGTLEDTVLEEGVKLDNLIQVGHNVRIGAHTAIAGCT"
BASE COUNT          211 a          315 c          344 g          150 t
ORIGIN      
        1 gcgcgaattc ggcgaactga acaaggattt tcagcgcaaa cagcgcgagt tccgcgagga
       61 cctcaaccag cggcgcaacg aagagctcgc cgcggtgctg gagcgcgcca accgcaccat
      121 caaggccatc gccgaggcgg agaaatacga catcatcttc caggaggcgg tctacgccag
      181 tccgcgcatc gacatcactg acaaagtgat caaggcactg gaagacaagc cggccgggaa
      241 atagccagcg ccgatggcta aggccaagaa ataccgcctg gcccagatcg tcgaacgctt
      301 cggtggggag atcgtcggca acccgcagac gccgatcagc ctggtcgcga cgctgcagag
      361 cgcgggtgcg caggacatca gttttctcag ccacagcaaa taccgcaagc agcttgcagg
      421 cacggacgcc ggcgcggtga tcctggcccg agctgatagc gacttgacgc cgttgccgcg
      481 catagtctgc gctgatccct atctgtattt cgccaaagtc tcgtcgctgt tcaacccgca
      541 gccggcggcc aggcccgggg tgcacaggag cgcggtggtc gagcgcggca cgaaagttcc
      601 agccagcgcc agcgtcggcg ccggtgcgta catcggccgc ggcgtgaaac tcggcaaagg
      661 cgtggcgatc ggcgccggct gctacgtcgg cgacgcggtc gagatcgggg ctggcagccg
      721 attgcacgcg cgcgtcgtga tctacgccgg ttgccgctta ggcgaacgcg tgcaggtgca
      781 ttcgggcgcg gtgatcgggg ccgacggctt cggcatcgcg tggaacggcg aagcctggag
      841 caagattccg cagatcggcg gcgtgctcat cggcgacgac gtcgagatcg gcgccaatac
      901 ctgcatagac cgcggcacgc tggaagatac cgtgctggaa gagggtgtga aactggacaa
      961 tctgatccag gtgggacata acgtacgcat cggcgcgcat accgcgatcg ccggctgcac
//
LOCUS       DASRVO010000273         1487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_8393, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000273 DASRVO010000000
VERSION     DASRVO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8393"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /gene="mrdA"
                     /locus_tag="VFF05_03360"
     CDS             <1..1471
                     /gene="mrdA"
                     /locus_tag="VFF05_03360"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZW50872.1"
                     /translation="KFAVNRYRFPGVDIQARLFRQYPLSDQASHVTGYIGRINERDLE
                     RIDESDLAANYKGTDHIGKTGLEQSYENELHGVTGYEQVEVDSAGRGVRTLSRTSPTP
                     GNNLVLTLDMKLQQVAEQAFGDKRGALVAIEPATGAILAFVSQPGFDPNLFVDGIDPA
                     SWDQLNNSPNRPMVDRALAGTYPPGSTFKPYMALAALELGKRTTQQTIFDPGYFNFGG
                     HRFRDDKPGGHGTVDMYKSLVMSCDTYYYMLGNDLGIDNIAHFIGQFGFGAKTGIDID
                     GEAAGVLPSQEWKRKRFRKPDQQKWYAGETISVGIGQGYNAYTPLQLAQAIATLANNG
                     VMYRPHLVNYIENTRSGERTQVEPKLERTIALKPANLEFIKRALAGVNIEGTGARAFA
                     KAEFTSGGKTGTAQVVAIKQNEKYNEKHVTERHRDHALFIAFAPLESPKIALAVVVEN
                     AGFGARAAAPIARQVLDYYLLGKSADRPAPEVAGDDGDD"
BASE COUNT          317 a          505 c          454 g          211 t
ORIGIN      
        1 caagttcgcg gtaaaccgtt accgcttccc cggcgtcgac atccaggcgc gcctgttccg
       61 ccagtacccc ctgagcgacc aggcttcgca cgtgaccgga tacatcggcc gcatcaacga
      121 gcgcgacctg gaacgcatcg acgagtcgga cctcgccgcc aactacaagg gcacggacca
      181 catcggcaag accggtctgg agcaaagcta cgagaacgag ctgcacgggg tcaccggcta
      241 cgagcaggtc gaagtcgact ccgccgggcg cggtgtgcga acgctctcgc gcacctcccc
      301 caccccgggc aacaatctgg tgctgacgct ggacatgaaa ctgcagcagg tcgccgagca
      361 ggccttcggc gacaagcgcg gcgcgctggt ggcgatcgag cccgccaccg gcgccatcct
      421 cgccttcgtt tcccagcccg gcttcgaccc caacctgttc gtcgacggca tcgatccggc
      481 gagctgggac cagctcaaca attcgccgaa ccgcccgatg gtggaccgcg cgctcgccgg
      541 aacctatccg cccggctcca cgttcaagcc ctacatggcg ctggcggcgc tcgaactggg
      601 caagcgcaca acgcaacaga cgatattcga ccccggctat ttcaatttcg gcggccatcg
      661 ctttcgcgac gacaagcccg gcggccatgg cacggtcgac atgtacaaat cgctggtcat
      721 gtcctgcgac acgtattact acatgctcgg caatgacctc ggcatcgaca atatcgcgca
      781 cttcatcggc cagttcggct tcggcgcgaa aaccggcatc gacatcgacg gcgaagccgc
      841 gggcgtgctg ccgtcgcagg agtggaagcg caagcgcttc agaaaacccg atcagcagaa
      901 atggtacgcc ggcgaaacca tcagcgtcgg catcggccag ggctacaacg cctacacgcc
      961 gctgcaactg gcgcaggcga tcgcgacgct ggccaataac ggcgtcatgt accggccgca
     1021 cctggtcaac tacatcgaga acaccaggag cggcgagcgc acccaggtcg agccgaaact
     1081 cgagcgcacc atagcgctga agccggcgaa cctggagttc atcaagcgtg cgctggccgg
     1141 cgtgaacatc gaaggtacgg gggcgcgcgc gttcgccaag gccgaattca ccagcggcgg
     1201 caagaccggc accgcgcagg tggtcgcaat aaagcaaaac gaaaaataca atgagaagca
     1261 cgtgaccgag cgccaccgcg atcacgcgct gttcatcgct ttcgcgccgc tggaaagtcc
     1321 caagatcgcg ctggcggtag tggtggagaa cgccggcttc ggcgcgcgcg cggcggcgcc
     1381 gatcgcgcgc caggtgctcg actactacct gctcggcaag agcgccgacc ggcctgcgcc
     1441 cgaagttgcc ggagacgacg gcgatgatta gacgcgtatt gaaaaag
//
LOCUS       DASRVO010000274         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_163694, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000274 DASRVO010000000
VERSION     DASRVO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163694"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="sbcB"
                     /locus_tag="VFF05_03365"
     CDS             complement(<1..722)
                     /gene="sbcB"
                     /locus_tag="VFF05_03365"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HZW50873.1"
                     /translation="MSAAASIYWHDYETSGTDARRDRPVQFAGQRTTLDLEPVGEPLS
                     ILCRPAPDVLPQPAACLITGIAPQRAMREGVGEAEFAAHIHEELRAPGTCGTGYNSIR
                     FDDEFTRNLLYRNFYDPYEREWKDGNSRWDIIDLARMCYALRPQGIEWPVNEKNQPSF
                     RLEHLTAANGIAHAQAHDALSDVQATIAFARLLKCAQPRLWDFYFSLRRKAEAFKYLD
                     YAHRTPVLHTSSRYPAERGCTA"
     gene            complement(745..>1509)
                     /locus_tag="VFF05_03370"
     CDS             complement(745..>1509)
                     /locus_tag="VFF05_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW50874.1"
                     /translation="LPEQHGALWTRYLRQHGQQQRMFYEILVRLRASDGSLVAPNAFL
                     PAAERFSMVADIDRWVIRHAFQALREQNRHDSPMGLSINLASQSLAHGNLGGFITDCL
                     VEFDVDPRAVIFEITEAGAINHLEATRHLIAELKPFGCRFALDDFGIGFSSFAHLKHL
                     DVDFLKIDGSFIRELLNDPVDLAVISAITNIAHSIGKIAVAEHVDRPEILRALHACGV
                     DQAQGFYIGKPQPTLRAANGQLELIQDNAATGGNAA"
BASE COUNT          270 a          442 c          522 g          275 t
ORIGIN      
        1 atcgccgtgc atccgcgttc ggcgggatag cgcgaagaag tgtgcagcac cggcgtgcgg
       61 tgcgcgtaat ccaggtactt gaacgcttca gccttgcggc gcaaggaaaa ataaaaatcc
      121 cacaggcgcg gctgtgcgca cttgagcagg cgcgcgaatg caatcgttgc ctgcacgtcg
      181 gacagcgcat cgtgtgcctg cgcgtgcgcg atgccgttcg cggcagtgag atgctccagc
      241 ctgaaactcg gctggttctt ctcgttgaca ggccattcga tgccctgcgg ccgcagcgcg
      301 tagcacatgc gcgccagatc gatgatgtcc cagcgcgagt tgccgtcctt ccattcgcgc
      361 tcgtagggat cgtaaaaatt gcgatacaga agattgcgcg tgaactcgtc gtcgaagcgg
      421 atcgagttgt agccggtgcc gcaggtgccc ggtgcccgca gttcttcgtg gatgtgtgcg
      481 gcgaattcgg cttcgccgac gccttcgcgc atcgcacgct gcggcgcgat gccggtaatc
      541 aggcaggccg ccggttgcgg cagcacgtcc ggcgcgggtc gacacaaaat ggacagcggc
      601 tcgccgacgg gttcaaggtc tagcgtcgtg cgctgaccgg cgaattgcac cggacggtcg
      661 cgccgcgcgt cggtgccgga agtctcgtaa tcgtgccagt agatgctcgc cgccgcactc
      721 atgcagtgca ggttagcacg caagtcaagc ggcgtttccg ccagtggcgg cattgtcctg
      781 gatcagttcg agctggccgt tcgcggcgcg cagcgtcggc tgcggtttgc cgatgtagaa
      841 gccctgcgcc tgatcgacgc cgcaggcatg cagggcgcgc aggatttccg ggcggtccac
      901 gtgctcggca acggcgatct tgccgatcga atgcgcgatg ttcgtgatcg cgctgatgac
      961 ggcgagatcg accgggtcgt tgagcagttc gcggatgaag gagccgtcga tcttgaggaa
     1021 atccacgtcc agatgcttca ggtgcgcgaa ggacgagaag ccgatgccga aatcgtccag
     1081 cgcgaagcgg cagccaaagg gtttcagctc ggcgatcaga tggcgcgtgg cttccaggtg
     1141 attgatcgcg cccgcttcgg taatctcgaa aatcaccgcg cgtgggtcga catcgaactc
     1201 gaccaggcaa tcggtgatga agccgccgag attgccgtgg gcgagggact gcgaggcgag
     1261 gttgatcgac agccccatcg gcgaatcatg gcggttctgt tcgcgcaggg cctggaaagc
     1321 gtggcggatc acccagcggt caatgtcggc gaccatgctg aagcgttcgg ctgcgggcag
     1381 gaacgcgttc ggcgcgacca gcgaaccgtc cgacgcgcgc aggcggacca ggatttcgta
     1441 gaacatgcgc tgctgctggc cgtgctggcg caggtagcgc gtccacaatg cgccgtgctg
     1501 ttcgggcag
//
LOCUS       DASRVO010000275         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_8414, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000275 DASRVO010000000
VERSION     DASRVO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8414"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFF05_03375"
     CDS             <1..909
                     /locus_tag="VFF05_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZW50875.1"
                     /translation="KGAIEAVLAHVAAPRSATIRGNAAVALVQDAAVQELRTLADGIA
                     RLGGTPLAVARNGQLLGVIHLKDIVKGGIRERFAALRTMGIRTVMITGDNPLTAAAIA
                     AEAGVDDFLAQATPEDKLKLIRAEQAQGKLVAMCGDGTNDAPALAQADVGVAMNTGTM
                     AAREAGNMVDLDSDPTKLIEIVEIGKQLLMTRGALTTFSIANDVAKYFAIVPAMFLAF
                     YPQLQALNIMRLQTPQSAILSAIIFNALIIIALIPLALRGVAYRPIGAASILRRNLLI
                     YGIGGIIAPFVGIKLIDMAVTAIGLA"
BASE COUNT          188 a          325 c          318 g          187 t
ORIGIN      
        1 aagggtgcga ttgaggcggt gttggcgcat gtcgccgcgc caagaagtgc aacgattcgg
       61 ggcaatgccg cggttgcgtt ggttcaggac gcggccgtgc aagaattgcg gacgcttgcc
      121 gacggcattg ccagattggg cggaacgccc ctcgcggtcg cccgcaacgg gcagctcctc
      181 ggcgtcatcc atctgaagga catcgtgaag ggcggcattc gcgagcgttt cgcggcgctg
      241 cgcacgatgg gcatccggac ggtcatgatc actggcgaca acccgctgac cgccgccgcg
      301 atcgccgccg aagcgggggt cgacgatttt ctcgcgcagg cgacgccaga ggacaagcta
      361 aagctgatcc gcgccgagca ggcgcaaggc aagctggttg caatgtgcgg cgacggcacc
      421 aacgacgctc cggcgctggc gcaggctgat gtcggcgtgg cgatgaacac cggcacgatg
      481 gcggcgcgcg aggccggcaa catggtcgat ctcgatagcg atcccaccaa gctcatcgaa
      541 atcgtcgaaa tcggcaagca gctgctgatg acccgcggtg ccctgaccac cttctcgatc
      601 gccaacgacg tggcgaagta ttttgcgatc gttccggcga tgttcctcgc gttctatccg
      661 caattgcagg cgctcaatat aatgcggctg cagactccgc agagcgcaat cctgtcggcg
      721 atcatcttca acgcgctgat catcatcgcg ctgatcccct tggcattgcg tggcgtggcg
      781 taccgcccga tcggcgccgc cagcatcctg cgccgcaacc tgctgatcta tggcatcggc
      841 ggtatcatcg cgccgttcgt cggcatcaaa ctgatcgata tggcggtgac cgctatcggt
      901 ctcgcctgat ctccgaggac gttctcccat gctgaaggaa atccgccccg ctatcatgat
      961 gaccgtcgtc atgaccgcaa tcaccggcct ggtctatccg ctcggcatca ccggcatc
//
LOCUS       DASRVO010000276         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_241692, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000276 DASRVO010000000
VERSION     DASRVO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241692"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFF05_03380"
     CDS             complement(<1..436)
                     /locus_tag="VFF05_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50876.1"
                     /translation="MQFMSRRIRCALVRAGLLAALLASAPALAATYCVSTASELQDAL
                     NQAALNTGEDNTIGLAPGYYLTADNGNAPFTFSSTTTHALYMGNFGYPTCDPLTSGPI
                     VLDGSGVSAVLSISSQGIIGISRLTIQNGRIPVEDTSGTYAAA"
     gene            553..>1056
                     /locus_tag="VFF05_03385"
     CDS             553..>1056
                     /locus_tag="VFF05_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZW50877.1"
                     /translation="MTEAAEITELLNAANAGDSDAREAAYAAVYEELMRRARNLRRRS
                     PDVSLTATELVHELFLRLDGARAARIASRAHFYALAARAMRQIVVDHARRRQSRKRGG
                     QIVFTGGDRAGEIGVASAEQALELDAALTELTRHDADLARVVEWHFFAGLSFREIGAE
                     LHRHERTV"
BASE COUNT          192 a          307 c          365 g          192 t
ORIGIN      
        1 cggccgccgc gtatgtgccg ctggtgtctt cgaccggtat acgcccgttc tgaatggtca
       61 ggcgcgaaat gccgattatt ccttggctgc ttatggacag cactgccgag acaccggaac
      121 catcgagcac aatgggtccg ctcgtcaatg ggtcgcaggt cgggtaaccg aagttgccca
      181 tatacaaggc gtgggtcgtt gtcgagctga aggtaaacgg cgcgttgccg ttgtcggcgg
      241 tcaagtagta tcccggtgcc aggcctatgg tgttgtcctc gccggtattg agtgctgcct
      301 gattgagggc atcctgcaac tcgctcgcag tcgatacgca gtaagtcgcc gccaaggcag
      361 gtgccgaggc aagcaacgcg gcgagaagtc cggcacggac gagggcacag cggatacggc
      421 gtgacatgaa ttgcatggcg gttctccggt cggatatcaa tccacataca cgcagatcgc
      481 ggggaaatcc tggtgcgaca agtacgtgcc agactgatat gctgatcgcg taaggcatcc
      541 gaaggccggg cgatgaccga agccgccgag atcaccgagc tgctcaacgc cgccaatgcc
      601 ggcgactcgg acgcacgcga ggcggcgtat gcggcggtgt atgaagaatt gatgcggcgc
      661 gcccggaatc tgcgccggcg atctcccgat gtgtcgctga ctgctaccga gctcgtgcac
      721 gaattgtttc tgcgcctgga cggcgcgcgg gccgcgcgca tcgccagccg cgcgcatttt
      781 tacgcgctgg cggcgcgggc gatgcgccag atcgtggtcg atcacgcgcg ccggcggcaa
      841 agccgcaagc gcggcggtca gattgtgttc accggcgggg accgcgccgg tgagatcggc
      901 gtcgccagcg ccgagcaggc gctggaattg gatgcggcgt tgaccgaact gacccggcac
      961 gacgcggatc tggcccgggt cgtggaatgg catttcttcg cgggtttgag tttccgcgag
     1021 atcggcgcgg aactgcatcg gcacgagcgt accgtt
//
LOCUS       DASRVO010000277         3256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_34813, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000277 DASRVO010000000
VERSION     DASRVO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34813"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VFF05_03390"
     CDS             complement(<1..1369)
                     /locus_tag="VFF05_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZW50878.1"
                     /translation="MKLQEDNIDFTTLNWVKQELDETLKQARQSLEAYVEDPADSSLM
                     RFCATYLHQVQGTLRMVELYGAAMVVEEMERVALALLDGQIKAKDDSYSVLMRGIVQL
                     PDYLERLQSGHKDIPIVLLPLLNDLRATRGEKLLTESVLFSPDLSAPLPAAAGGAAQA
                     TPELDLKAEAARLRSAFQLSLLRWIRDDSASVQLTRLIEILDRLRTACTQEDACRLWW
                     VSAGVLEGVASSAVEANAAVKLLFGKVDREIKRLVDTGEAGFRVSPPADLTKNLLFYI
                     AHSAAAGERAAAIRATYKLDTLLPSEKELEHAKGSISGHNRSLLDTVSVAIKDDLMRV
                     KEALDIFLRAQSPDPTELMAQVDALDRVGDTLGMLGLGVPRRVVTEQRKVVEEMANKS
                     RPADETTLLDVAGALLYVEASLDDHIDRLGADSGEQTGGGPGSQGLELPKAEARKILD
                     ALMKEA"
     gene            complement(1380..2249)
                     /locus_tag="VFF05_03395"
     CDS             complement(1380..2249)
                     /locus_tag="VFF05_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HZW50879.1"
                     /translation="MSVAAGTFSPLMAMPGMDDSEFERWASLLEKRTGVVVPLKRKAF
                     LVTSVRGRMRETGHADFESYFRELQKIPEGAIEWTTLVDRLTVHETHFFRHPPSFDLI
                     ARDWLPQLAADPARSALHAWSVGCSSGEEAYTLAMVLDRHLAARGGKSYFGVTATDVS
                     LPALAVGRAGLYPRQKMDEIPEAYREQHCEALDADSFRIREALRKRVGFAQFNLLDAV
                     GASMKRLDLIFCQNVLIYFARERRRDLLATLANLLKPGGLLVLGPGEVTQFAHAQLAR
                     VDNRNVLAFRRTS"
     gene            complement(2347..>3256)
                     /locus_tag="VFF05_03400"
     CDS             complement(2347..>3256)
                     /locus_tag="VFF05_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZW50880.1"
                     /translation="EDITGAIADSVNFAVEALRSLVTTINETAVQVSAAAQETQATAM
                     HLAEAAEHQAQQITSASAAINEIAVSIDEVSKNSAESADVAQRSVQIASKGAAIVRQT
                     IQGMDSIRDQIQETSKRIKRLGESSQEIGSIVELINDIAEQTNILALNAAIQAASAGE
                     AGRGFAVVADEVQRLAERASGATRRIETLVQTIQADTNEAVTSMEQTTSEVVAGARLA
                     EDAGLALGEIEKVSNDLADLIQNISEAARQQSAAATNISATMNVIQEITTQTSVGASQ
                     TAESIGNLAQLAADLRRSVADFKLPA"
BASE COUNT          531 a         1068 c         1045 g          612 t
ORIGIN      
        1 tggcttcctt catcagcgca tcgagtatct tgcgcgcttc ggccttgggc aattccagac
       61 cctgcgatcc ggggccgccg ccggtttgct cgccgctatc cgcgccgagg cggtcgatgt
      121 ggtcgtccag cgaagcttcg acataaagca gcgcgccagc cacgtcgagc agggtcgtct
      181 cgtcggcggg acgcgacttg ttcgccatct cctcgacgac cttgcgctgt tcggtgacca
      241 cgcgccgcgg cacgcccaga ccgagcatgc cgagggtgtc gccgacgcgg tcgagcgcat
      301 cgacctgcgc catgagttcg gtgggatcgg gactttgcgc gcgcaggaaa atatccaatg
      361 cctctttcac gcgcatcaga tcgtccttga tcgccaccga caccgtgtcg agcaggctgc
      421 ggttgtggcc ggaaatcgac cccttggcgt gttcgagttc cttctcgctc ggcaacaggg
      481 tatcgagctt gtaggtggcg cgaatcgccg cggcacgttc gccggcggca gctgagtgag
      541 cgatgtagaa cagcagattc ttggtcagat cggccggcgg cgaaacgcgg aagccggcct
      601 cgccggtatc gaccaggcgc ttgatctcgc gatcgacctt gccaaacagc agcttgactg
      661 ccgcattcgc ctcgaccgca ctggaagcga cgccctcgag cacgccggcg gaaacccacc
      721 acagccggca agcgtcttcc tgcgtgcagg ccgtgcgcag gcggtcgagg atctcgatca
      781 ggcgcgtcaa ttgcacgctc gcggagtcgt cacgaatcca gcgcagcaac gacaactgga
      841 acgcggagcg caggcgtgcc gcttcggcct tgaggtcgag ttccggcgtg gcctgcgcgg
      901 cgccgccggc ggcggcgggc agtggcgcgg acagatccgg cgagaacagc accgactcgg
      961 tgagcagttt ctcgccgcgc gtcgcgcgca aatcgttgag caggggcagc aggacgatgg
     1021 gaatgtcctt gtgtccgctc tgcaggcgtt ccaggtaatc gggcaactgc acgatgccgc
     1081 gcatgagcac ggaatagctg tcgtccttgg ccttgatctg gccatcgagc agggccagcg
     1141 cgacgcgttc catttcttcc acgaccatcg ccgcgccgta cagctcgacc atgcgcaagg
     1201 tgccctgcac ctggtgcagg taggtcgcgc agaagcgcat cagactcgag tcggccggat
     1261 cctcgacgta ggcctcgagc gactggcgcg cctgcttgag ggtctcgtcg agttcctgct
     1321 tgacccagtt gagggtggtg aagtcgatgt tgtcttcttg cagcttcatg gtctttctct
     1381 caactcgtgc gccggaaggc cagcacattg cgattgtcca cgcgcgcgag ttgcgcgtgc
     1441 gcgaactgcg tcacttcgcc cggcccgagc accagcaggc cgccgggctt gagcaggttc
     1501 gcgagcgttg ccaacaggtc gcggcgccgc tcgcgggcga agtagatcaa tacgttctgg
     1561 cagaaaatca ggtccagccg cttcatcgac gcaccgactg cgtcgagcag gttgaactgg
     1621 gcgaaaccca cccgtttgcg caacgcttcg cggatgcgga agctgtccgc gtccagcgcc
     1681 tcgcagtgtt gctcgcggta cgcctccgga atttcgtcca ttttctgccg tgggtaaagc
     1741 ccggcgcgac caacggccaa agccggcaga ctcacgtccg tcgcggtcac gccgaaatac
     1801 gatttgccgc cgcgcgcagc caggtgccgg tccagcacca tcgccagcgt gtaggcttcc
     1861 tcgccgctgg agcagcccac gctccaggca tgcagcgcgc tgcgagccgg atccgcggcg
     1921 agttgcggca gccagtcgcg tgcgatcaga tcgaacgacg gcggatgccg gaagaaatgc
     1981 gtctcgtgca cggtcaggcg atcgaccagt gtggtccact cgatcgcgcc ttcgggaatt
     2041 ttctgcagtt cgcggaaata ggattcgaaa tccgcatgtc cggtctcgcg catgcgcccg
     2101 cgcacgctgg tgaccaggaa ggccttgcgc ttgagcggca cgacgacgcc cgtgcgcttc
     2161 tcgagcagac ttgcccagcg ctcgaactcg ctgtcgtcca tgcccggcat ggccatcagc
     2221 ggcgagaaag tcccggcggc aaccgacatg cgcgatacca atctccacca acccgtacat
     2281 ccgaacttcc gcgttcatgg acaaagtccc tcgccgcatg cacttcgcga cgcttcgtct
     2341 ggccctttac gccggcagct tgaagtccgc caccgaacgg cgcaggtccg cggccagttg
     2401 cgccaggttg ccgatcgact cggcggtctg gctggcgccg accgaagtct gcgtggtgat
     2461 ttcctgaatc acgttcatgg tcgcggaaat gttcgtcgcc gcggccgact gctggcgcgc
     2521 cgcttcggaa atgttctgaa tcaggtcggc aaggtcgttc gagacctttt cgatctcgcc
     2581 cagggccaag cccgcgtcct cggccaggcg tgcgccggcc accacttcgg acgtggtctg
     2641 ttccatcgag gtgaccgctt cgttggtgtc ggcctgaatc gtctgcacca gcgtttcgat
     2701 tcggcgtgtg gcgccggaag cgcgttcggc cagtcgctgc acttcgtcgg cgaccaccgc
     2761 gaagccgcgg cccgcctcac cggcggacgc ggcctgaatg gcggcgttca aggccagaat
     2821 gttcgtctgt tccgcaatgt cgttaatcag ttcgacgatg gaaccgattt cctgcgagga
     2881 ttcgccgagg cgcttgatgc gtttcgacgt ttcctggatc tggtcgcgaa tcgagtccat
     2941 gccctggatc gtctggcgca cgatggccgc gcccttcgag gcgatctgca ccgagcgctg
     3001 cgcgacgtcg gccgattcgg ccgagttctt cgacacctcg tcgatcgaca ccgcgatctc
     3061 gttgatcgcc gctgacgcgg aagtgatctg ctgggcctga tgctcggcgg cttcggccag
     3121 gtgcatcgcg gtggcctgcg tttcctgtgc ggcggcggac acctgcacgg cggtctcgtt
     3181 gatcgtcgtc accagggagc gcagcgcctc aaccgcgaaa ttgaccgagt ccgcaatcgc
     3241 cccggtgatg tcctcg
//
LOCUS       DASRVO010000278         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_163783, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000278 DASRVO010000000
VERSION     DASRVO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163783"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFF05_03405"
     CDS             complement(<1..618)
                     /locus_tag="VFF05_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZW50881.1"
                     /translation="MLDDVLRPDDQALSRTTRDLTAEARAGRLEAVRCRDDEIARVVD
                     ILLRQSKNNPALVGPAGVGKTAIAEGLAQRIASGDVPLALRDVRLLGLDHVALLAGTM
                     YRGQYEERLRDIVNTASSDPNVILFIDELHNLIGQGTAMGAAMDAANMLKPALVRGEF
                     RVIGATTQDEYDHWVRGDPALERRFQKITVRELTVEQTLEVLHARK"
     gene            complement(618..>1051)
                     /locus_tag="VFF05_03410"
     CDS             complement(618..>1051)
                     /locus_tag="VFF05_03410"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZW50882.1"
                     /translation="GLVWALILAITAAVMRETSVFANLYDSAIGACVGAGVIAIAGWL
                     GEMAFKKEAMGFGDVTLMAMVGAHLGIARTFLTVFFGAALGAVIFLLVVFPISYVRSR
                     RAGVEFDAPLVPFGVFLAPAAMIALLWGGPLIAWYRGLTGI"
BASE COUNT          191 a          378 c          321 g          161 t
ORIGIN      
        1 cttgcgcgcg tgcaagactt ccagtgtctg ctccaccgtc agctcgcgca ccgtgatctt
       61 ctgaaagcgg cgctcgagcg ccgggtcgcc gcgcacccag tggtcgtact cgtcctgcgt
      121 ggttgcgccg atcacgcgga actccccgcg cacgagcgcc ggcttgagca tgttggcggc
      181 gtccatcgcc gcacccatcg cggtgccctg cccgatcagg ttgtgcagct cgtcgatgaa
      241 caggatgacg ttgggatccg acgatgccgt gttcacgatg tcgcgcagtc gctcctcgta
      301 ctggccgcgg tacatcgtgc cggcgagcag cgcgacgtgg tcgagcccga gaaggcgcac
      361 gtctcgcaac gccagcggca cgtcgcccga cgcgatccgc tgcgccagcc cttcggcgat
      421 cgccgtcttg ccgacccccg ccggtccgac caacgccgga ttgttcttgc tctggcgcag
      481 caagatgtcg acgacgcgcg cgatctcgtc atcgcgacac cgcaccgctt cgagccggcc
      541 ggcgcgcgct tcggcggtga ggtcgcgcgt ggtgcgcgag agcgcctggt catcgggtcg
      601 caacacgtcg tccagcatca gattcccgtt agtcctctat accaggcgat gagcgggccg
      661 ccccacaaca gcgcgatcat ggccgcgggc gccagaaata cgccgaacgg aacgagcggc
      721 gcgtcgaact cgacgccggc gcgccgactg cgcacgtacg agatcgggaa caccacgagc
      781 agaaagatca cggcgccaag cgccgcgccg aaaaatacgg tcaaaaaggt ccgggcaatc
      841 cccaagtgcg cgccgaccat ggccatgagc gtcacgtcgc cgaatcccat cgcttccttc
      901 ttgaacgcca tctcgccaag ccatccggcg atggcgatga cacccgcacc gacgcacgcc
      961 cctatcgctg aatcatacag gttggcgaac accgaagttt cgcgcatcac cgcggccgta
     1021 atggccagaa tcagcgccca gaccaacccg a
//
LOCUS       DASRVO010000279         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_216423, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000279 DASRVO010000000
VERSION     DASRVO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216423"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>1134
                     /locus_tag="VFF05_03415"
     CDS             13..>1134
                     /locus_tag="VFF05_03415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HZW50883.1"
                     /translation="MARVYAKVEAMLAEKDFGTFGHMILRAVQLLRDDPKSLARERAG
                     ARFILIDEFQDANVAQIMLAELLAGAERNLFAVGDPDQAIYRFRGATNAAFDEFARRF
                     PDAKGVVLNANRRSGAYILDVAYSAITCNPDVAIALPGGGSFHRERLTSVREANGAHL
                     PPVAIVVCPGSKDDGHREEAAHVAEAIECLRSQPASGSGKPRIGVLYRSHSNRDFLVE
                     QLAVRNIPFAVKGINALETAEVRNLLACFTVMHTPAEGESLLRVAALPQFALDPLQVR
                     DELAAAPRGTSFVSLLPKIPGGERVLAAVERARGLAAAADWEVQKAVRIAIAGFSFDA
                     DHPAIRALIAFVAKWKDKAIAGDGKLAGFLAYMDYFDEAS"
BASE COUNT          175 a          412 c          348 g          199 t
ORIGIN      
        1 cgctgccggg agatcgcgcg tgtgtacgct aaggtcgaag ccatgctcgc cgaaaaagat
       61 ttcggcacct tcggccacat gatcctgcgc gcggtgcagc tgttgcgtga cgatccgaaa
      121 tcgctggccc gcgagcgtgc cggcgcccgc ttcatcctga ttgacgagtt ccaggacgcc
      181 aacgtggccc agatcatgct cgccgaactg ctggcgggcg cggagcgcaa cctcttcgcg
      241 gtcggcgatc ccgaccaggc gatctatcgt tttcgcggcg ccaccaacgc cgccttcgac
      301 gagtttgcac gccgctttcc cgacgccaag ggcgtggtgc tgaatgccaa ccgccgctcc
      361 ggcgcctaca tcctcgacgt cgcctactcg gccattacct gcaatcccga cgtcgccatc
      421 gccctgcccg gcggcggcag ctttcaccgc gagcgcctga cgtccgtccg cgaagccaac
      481 ggcgcgcacc ttccgccagt cgccatcgtg gtctgtcccg gctcgaagga cgatggacac
      541 cgcgaagaag ccgcgcacgt cgccgaggcc atcgagtgcc tgcgctcgca gcctgcgtcg
      601 ggcagcggca agccgcgcat cggcgtgctt taccgcagcc actccaaccg cgacttcctg
      661 gtcgagcagc tcgcggtgcg caatattccc ttcgcggtca agggcatcaa cgcgttggaa
      721 accgccgagg tgcgcaacct gctcgcctgc ttcacggtga tgcacacgcc ggcggagggc
      781 gagtcgctgc tccgcgtcgc cgcgctgccg cagtttgcgc tcgatccgct gcaggtgcgc
      841 gacgaactgg ccgccgctcc gcgcggcaca tcgttcgtgt ccctgctgcc caaaattccc
      901 ggcggtgagc gcgtgcttgc cgccgtggaa cgcgcccggg gcctcgccgc tgccgccgat
      961 tgggaggtgc agaaagcggt ccgcatcgcc attgccggct tctccttcga cgccgatcat
     1021 cccgccattc gcgcgctgat cgcctttgtc gctaaatgga aagacaaggc catcgccggc
     1081 gatggcaaac tggccggctt cctcgcctac atggattatt tcgacgaggc ttcc
//
LOCUS       DASRVO010000280         1372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_34909, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000280 DASRVO010000000
VERSION     DASRVO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34909"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFF05_03420"
     CDS             <1..182
                     /locus_tag="VFF05_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50884.1"
                     /translation="LTGGGLRPGLTQVMNNTVRRARDDLSDLARELEDAQLAAKQTMK
                     RTVAQVKKAVKSARR"
     gene            602..>1372
                     /locus_tag="VFF05_03425"
     CDS             602..>1372
                     /locus_tag="VFF05_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50885.1"
                     /translation="MTGDPSDAQHFYFGSVNGGVWETHDAGRTWQPIFDRESVGSIGA
                     IALAPSNSKTIYVGTGEADMRSDISQGDGMYKSDDGGKSWTHIGLTDTQQIERILVDP
                     ADAQHVYVAALGHPYGPNEQRGVFASRDGGKTWQKLLGPNADTGAIDLAFEPGNPSIV
                     YAALWQTRRPPWNVYPPSNGPGSGLYKSTDGGAHWTQIKGHGFAGGTIGRIGIAISKA
                     APKRVYAIVDGDEGGLYRSDDAGATWMKTTGDARIWQRG"
BASE COUNT          257 a          456 c          419 g          240 t
ORIGIN      
        1 agctcaccgg cggcggcttg cgcccgggcc tgacccaggt catgaacaac accgtgcgcc
       61 gcgcccgcga cgatctttcc gacctcgcgc gcgagctcga ggacgcgcag ctcgccgcca
      121 aacagaccat gaagcgcacc gtcgcgcagg tcaagaaggc ggtcaagtcc gcgcgccgtt
      181 gatcgtttaa acccgccagc cacaacaaag ccgaaggatc cctcccccca taggctttgg
      241 ctggcgggtt ctttgttcaa gctccggaca agccatcctg acttgccgga gcgcgccgcc
      301 agcgccggtc cacgccgcgc tccggcgacc tgcctgattc aatccagcat caggccttcg
      361 ctcgcgacgg ttggacaagg cgctcattcg gtttagccat tacttctgtc cggttgccgg
      421 atcgctgctc ctgaacgatc atgccggttt gccttgacgg ggaaaccgca tgttcagatt
      481 tggcgcagca accgccctga tgctcctcgc cgccgcgtcc gcatctgcga tggacacggc
      541 tcaattgggc aacttgcatt ggcgactagt cggcccgttt cgtggtggcc gcacactggc
      601 ggtgaccggc gatccatcgg acgcgcagca tttctatttc ggatcggtga atggcggcgt
      661 atgggaaacg catgacgccg gtcgcacctg gcagccgatt ttcgaccgcg aatcggtagg
      721 ctccatcggc gcgatcgcgc ttgctccttc caattcaaaa acgatttacg tcggcaccgg
      781 cgaagcggac atgcgctccg acatttcgca aggcgacggc atgtacaaat ccgacgacgg
      841 cggcaagagc tggacgcata tcgggttgac cgatacgcag cagatcgaac gcattctcgt
      901 cgaccccgcc gatgcgcaac acgtttatgt cgccgcgctc ggccaccctt acggaccgaa
      961 cgaacagcgc ggcgtgttcg ccagtcgcga tggcggcaag acctggcaga aattgctcgg
     1021 ccccaatgcc gacaccggcg cgatcgacct cgccttcgaa ccgggtaatc cgtcgatcgt
     1081 ctacgcggcg ttgtggcaga cgcggcgccc gccgtggaat gtgtacccgc cttcgaacgg
     1141 gcccggctcg ggcttgtaca aatccaccga tggcggcgcg cactggacac agatcaaggg
     1201 gcacggcttt gccggaggaa caatcggccg catcggcatc gcgatttcaa aagccgcgcc
     1261 gaagcgcgtc tacgccatcg tcgatggcga tgagggcggc ttgtatcggt ccgacgatgc
     1321 cggcgcgacg tggatgaaaa cgaccggtga cgcgcgcatc tggcagcgcg gc
//
LOCUS       DASRVO010000281         1203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_86568, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000281 DASRVO010000000
VERSION     DASRVO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86568"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VFF05_03430"
     CDS             complement(<1..1183)
                     /locus_tag="VFF05_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW50886.1"
                     /translation="MKILVLDGLMIVGQVSVMLYNSARLTLALFVLAPPVALVVWYVG
                     KRYRRISHRIQDSVGSITGMVDEVVGGQREVRIYGGREYERSRFAVVNDAIRRLNLKI
                     ASTNSLANAVVQFVAAFALALVVFLATRPGAILREMSPGTFMSLITAMLVMLPSLKRL
                     TTVQSNMQRGVVAAQDIFSVLDTDEEHDAGNFRVERARGEIALDGVTFSYPNASVPAL
                     HGIDLQCAAGSITALVGRSGSGKSTLASLIPRFHDPDAGRILLDGRPLADYALDNLRA
                     QIAWVGQQVVIFDDTVARNIAYGALGGASREAIVEAARAANALDFIDALPNGMDSRVG
                     EGGALLSGGQRQRIAIARAILKDAPILILDEATSALDSESESLIQDALKRLLRQRTVL
                     VI"
BASE COUNT          208 a          407 c          393 g          195 t
ORIGIN      
        1 cgatcacgag caccgtgcgt tggcgcagca ggcgcttgag cgcgtcttgg atcaggcttt
       61 cgctttcgct gtccagcgcg ctggtcgctt cgtccaggat caggatcggc gcgtccttga
      121 ggatcgcgcg cgcaatagcg atgcgctggc gctggccgcc ggacagcagc gccccgccct
      181 cgcccacgcg gctgtccatg ccgttcggca gcgcgtcgat gaaatccagc gcgtttgccg
      241 cgcgagccgc ctcgacgatg gcttcgcgcg aggcgccgcc aagcgcaccg taagcaatgt
      301 tgcgtgcgac cgtgtcgtcg aagatcacga cttgctgacc gacccaggcg atctgcgcgc
      361 gcagattgtc gagtgcgtaa tccgccaatg gcctgccatc gagcaggatt cggcccgcat
      421 ccggatcgtg aaagcgcggg atcaggctgg ccagcgtgga cttgccgctg cccgaacgcc
      481 cgaccagggc ggtgatgctg cccgccgcgc actgcaggtc gatgccgtgc aacgcgggaa
      541 ccgaggcatt cggataactg aacgtcacgc catccagcgc gatctcgccg cgtgcgcgct
      601 cgacgcggaa gttgccggca tcgtgctctt cgtccgtgtc gagcacgctg aagatgtcct
      661 gcgccgcgac cacgccgcgc tgcatgttgg attgcaccgt ggtcaggcgc ttgagcgacg
      721 gcagcatgac cagcatcgcc gtgatcagcg acatgaacgt gcccgggctc atctcgcgca
      781 ggatcgcgcc gggccgggtg gcgaggaaca caaccagcgc cagcgcgaac gcggcgacaa
      841 actgcacgac cgcattcgcc agcgaattgg tcgaggcgat cttgaggttc aggcgccgga
      901 tcgcgtcgtt caccacggcg aagcggctgc gctcgtactc gcgtccgcca taaatcctga
      961 cctcgcgctg gccgccaacg acctcgtcga ccatgccggt aattgacccg accgaatcct
     1021 ggatgcgatg gctgatgcgc cggtagcgct tgcccacgta ccaaacgacc agcgccaccg
     1081 gcggagcgag cacgaacagc gccagggtca ggcgcgcgct gttgtacagc atcacgctga
     1141 cctggccgac gatcatcagg ccgtcgagca cgaggatctt caagccatcc gtgctggcgt
     1201 tgg
//
LOCUS       DASRVO010000282         1479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_86569, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000282 DASRVO010000000
VERSION     DASRVO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86569"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFF05_03435"
     CDS             complement(<1..349)
                     /locus_tag="VFF05_03435"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZW50887.1"
                     /translation="MNPAPNLFLIGPMGAGKSTLGKRVADALGLPFFDLDHEIQTQCG
                     ADIALIFDIEGECGFRRRESAMLAEFAAREGIVLATGGGAILDAHNRATLRERGFVVF
                     LETTVDDQLARLAR"
     gene            430..927
                     /gene="xrtH"
                     /locus_tag="VFF05_03440"
     CDS             430..927
                     /gene="xrtH"
                     /locus_tag="VFF05_03440"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HZW50888.1"
                     /translation="MIRFIVVFLVCLLVLFTAELLQPVQAHVIMPFTAGVAHVSAWIM
                     LLFDHNVIARGNDILSARSGGGIEIVAGCNGVEAVLILVSAILAFPSPWKHKLAGMAI
                     GFLAIQSLNVVRIISLFYLREWNMVWFDWFHLYLWQALIILDALVFWLIWLRWLPRTS
                     RIATA"
     gene            974..1180
                     /locus_tag="VFF05_03445"
     CDS             974..1180
                     /locus_tag="VFF05_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZW50889.1"
                     /translation="MNKITKVIELSASSTKGIEDAVTGGLKRASKTVKNIKGAWINDL
                     KVVTNDDGSVAEWRVNMRVNFLVD"
     gene            1181..>1479
                     /locus_tag="VFF05_03450"
     CDS             1181..>1479
                     /locus_tag="VFF05_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZW50890.1"
                     /translation="MTDALLVEAVAAVRELIDAARVAGDPEPTAMTLATSDARERLSA
                     RIVLLKELDERGLTFYTNYESAKASQLGEQQQAALCLHWKTLRDGVQVRAEGVVE"
BASE COUNT          271 a          458 c          476 g          274 t
ORIGIN      
        1 cgcgcgcgag gcgtgcgagc tggtcgtcca ccgtggtttc cagaaacacc acgaagccac
       61 gttcgcgcaa tgttgcgcgg ttgtgggcgt ccagaatcgc gccgccgccg gtcgccagca
      121 cgatgccctc gcgcgcggcg aattcggcca gcatcgcgct ttcgcggcgg cggaatccgc
      181 attcgccctc gatgtcgaag atcagcgcga tgtcggcgcc gcattgcgtc tggatttcat
      241 gatccaggtc gaaaaacggc aggccgagcg cgtccgccac gcgcttgccg agcgttgact
      301 tgccggcgcc catcgggcca atgaggaaca ggttgggggc aggattcatg gccaaatcct
      361 agcaggtggc ggcggatgtt tgcgtgccgc accgcgctac aataccgact ttcgtcccga
      421 actttctcca tgatccgttt catcgtcgtt tttctcgtct gcctgctcgt gttgttcacc
      481 gccgaactgc tgcaaccggt gcaagcgcac gtgatcatgc cgttcaccgc gggggtcgcg
      541 catgtcagcg catggatcat gctgctgttc gaccacaacg tgatcgcgcg cggcaacgac
      601 atcctcagcg cgcgcagcgg cggcggcatc gagatcgtcg ccggctgcaa cggcgtggag
      661 gcggtgctga tcctggtgtc ggcgatcctc gcgtttccgt cgccgtggaa acacaagctg
      721 gccggcatgg cgatcggatt cctcgccatc cagagcctga acgtggtgcg catcatcagc
      781 ctgttctacc tgcgcgaatg gaacatggtg tggttcgact ggttccattt gtatctgtgg
      841 caggcgctga tcattctcga tgcgctggtg ttctggctga tctggctgcg ctggctgccg
      901 cgcacatcgc ggatcgccac ggcctgatac actcgggcgg ctgcctggca gcaaacttcc
      961 aaggagtcta gccatgaaca agatcacgaa agtcatcgaa ttgagcgctt cgtcgaccaa
     1021 aggcatcgag gatgccgtga ccggcggcct gaagcgggcg agcaagacgg tgaagaacat
     1081 caagggcgcc tggatcaacg acctcaaggt cgtcaccaac gacgacggtt ccgtcgccga
     1141 atggcgcgtg aacatgcgcg tcaatttcct cgtcgactga atgaccgacg cactgctcgt
     1201 cgaggccgtc gcggcagtgc gcgagctgat cgacgcggcg cgcgttgccg gcgatccaga
     1261 acccacggcg atgacgctgg cgaccagcga tgcgcgcgaa cgcctgtccg cgcgcatcgt
     1321 gctgctcaag gaactggacg agcgcggctt aacgttctac acgaactacg aaagcgccaa
     1381 ggcttcgcaa ctcggcgagc agcagcaggc agcattgtgc ctgcactgga agacgctgcg
     1441 cgacggcgtg caggtgcgcg ccgaaggcgt ggtggaaaa
//
LOCUS       DASRVO010000283         7751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_60616, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000283 DASRVO010000000
VERSION     DASRVO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60616"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /gene="rpoD"
                     /locus_tag="VFF05_03455"
     CDS             complement(<1..1439)
                     /gene="rpoD"
                     /locus_tag="VFF05_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZW50891.1"
                     /translation="MAIENTNPQQSEIKTLIIKGKEQGYLTYAEVNDHLPDDIVDPEQ
                     IEDIIGMINDMGIEVHEIAPDTETLLPENPVATDDETATEEAVAVLSAVDAEVGRTTD
                     PVRMYMREMGTVELLTREGEIAIAKRIEEGLGQVQTALATFPWSIKLLVEEYDQHLEG
                     KKRLSEVLTGFADIEVPGMDDAMLEAEPAQAEAEEEEKENGETEEAGPGETGPDPAEV
                     TRRMDELRSLYAKFQKAAEKYGDGDKKTQKARDTMAEAFLKLKLPSIMIDAFVRKLRE
                     VVATIRGHERVIMDICVRQAKMPRKDFLKAFTAYEVNLNFADEVIRKKQKWASSIRAH
                     KADIVAEQEKLIALEKTLCLSLTDIKEINRAMSIGEAKARRAKKEMVEANLRLVISIA
                     KKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQART
                     IRIPVHMIETINKLNRISRTMLQEMGREPT"
     gene            complement(1499..1936)
                     /gene="dtd"
                     /locus_tag="VFF05_03460"
     CDS             complement(1499..1936)
                     /gene="dtd"
                     /locus_tag="VFF05_03460"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZW50892.1"
                     /translation="MIALIQRVTTASVDVDGATIARIGPGLLALVAVQPDDGETQAQR
                     LCERLLGYRVFADTNGKMNRSLADTGGGLLLVPQFTLAADTASGMRPSFTAAATPDAG
                     RRWFERLVEIAKNRHSTVEIGRFGAHMQVSLINDGPVTFWLET"
     gene            1987..2868
                     /locus_tag="VFF05_03465"
     CDS             1987..2868
                     /locus_tag="VFF05_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HZW50893.1"
                     /translation="MRWHVATLYFLLRLTGRLPLRALHGIGAGLGWLLWRLPNPLRRK
                     AVQTLSHVTTQIGGKNSHALLKPALIETGKGALELCKIWSGDPQAALGLIREIRGTEH
                     FDAALAAGKGLLIAAPHLGCWELLNFWLCSRTPIAIAYRPPRRAEIEPLLIRARGGLA
                     AEQVRAEGAAGVRALFKRLSAGGVIGILPDQQPKAGEGQFAPFFGTPALTMVLLSRLA
                     QRTGATVLFAFAERLPKGAGFRIHVLPAPEGIADADLPTAIAALNRRVEACVCIAPEQ
                     YQWAYKRYSIRPPQEHG"
     gene            complement(2870..3193)
                     /locus_tag="VFF05_03470"
     CDS             complement(2870..3193)
                     /locus_tag="VFF05_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW50894.1"
                     /translation="MSARLDSLRALCSGPRDGALLRVALANALVAEGMAVEAIAELRR
                     ALEFDPDYSAAWKQLGKLLAESGDATAAIETYRSGIAAAQKRGDKQVEKEMQVFLRRL
                     EKSGA"
     gene            complement(3190..4416)
                     /locus_tag="VFF05_03475"
     CDS             complement(3190..4416)
                     /locus_tag="VFF05_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZW50895.1"
                     /translation="MSPARARGAKKSTNPQRRKSTKTPAAAVRDYAHVALVLQGGGAL
                     GAYQCGVYEGLHEAGIAPNWFAGISVGAINAALLAGNPPQHRVDRLREFWERISTPAL
                     PLARRTAGWRRQWMSAFAAAEPWRSLANAASALDALAFGQSGFFVPRPPATLWNAHDP
                     ATASIYSTAPLKRTLEELVDFDRINHRTAPHLAVGAVNLRSGNFIYFDNRATKIRAEH
                     VMASGALPPGFPAVEIDGEFYWDGGVVSNTPLEYVIDSEPQGDMLALQVDLWSARGEL
                     PRNLADVFERQKDIQYSSRTRHGTDRLAERQHLRTALRKLAHALPGKRLPAPLARELA
                     PWLGEACLNVVHLIYSAKPYEEQYKDYAFGAATMREHWAAGLADMRASLAHPDWFVPP
                     PSSIGVATHDIHRGRP"
     gene            complement(4413..5195)
                     /locus_tag="VFF05_03480"
     CDS             complement(4413..5195)
                     /locus_tag="VFF05_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HZW50896.1"
                     /translation="MSVNNKVALVTGAASGIGKRIAEVLADNGAHIAIADINQAAADA
                     AAAEIRTRGVKASAVLMDVTNEEQVEAGVAKTVKDLGGVDILVSNAGIQIVYPIEEFP
                     FADWKKLLAIHLDGAFLTTRACLKRMYAAGKGGSVIYMGSVHSKEASKLKAPYVTAKH
                     GLIGLAKVVAKEGAAHGVRANVICPGFVRTPLVEKQIPEQAKELRISEDDVIKKVMLK
                     DTVDGEFTTVDDVAACALFFAGFETNALTGQSIVVSHGWFMQ"
     gene            complement(5218..6594)
                     /locus_tag="VFF05_03485"
     CDS             complement(5218..6594)
                     /locus_tag="VFF05_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZW50897.1"
                     /translation="MSAGRVLIVDDEPDIRSTVKEILEDEGYVAILAANADEARLARE
                     RERPDAILLDIWMPGTDGIALLREWAAAGVLAACPVVMMSGHGTVETAVEATRLGAWD
                     FIEKPISLAKLLLTLERAREASRLRHENEGLRQRLALPLEPVGSSKPMAALRAQLQKL
                     AQHDASVLIRGEAGTGKETLARWLHAHGPRRNGPFVAVAAGSIPRDHLVAALFGTEDA
                     SGVQPGLLEQANGGTLFLDEVADLDAELQQRLFSVLQRRALVRGGGREPAPLDLRVIA
                     ASARDLDAQVKAGAFREELYYLLNVLPLSVPPLRERAEDVPELLHLYADFFAGRDRLH
                     CRRFAHAAQKRLCAYAWPGNVRELRNLVQRLLVMGGAADVEPAEVEAALGAVASAVAP
                     STAGIDFDLPLREAREQFERAYLLHQLQQAGGSVGKLAKAAGMERTHLYRKLRDLGVD
                     VKNAVRED"
     gene            6706..>7751
                     /locus_tag="VFF05_03490"
     CDS             6706..>7751
                     /locus_tag="VFF05_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZW50898.1"
                     /translation="MAASTTPLLIRFGRLGDMLMQEPLLHLLRRRYGAPCRILTRGTW
                     TGALYAAHPDIGEIRQLHSRHAPLAFSPERWRAIAALRAHEGPVYVSEDTDRSLMRIG
                     WMLRLARVPRDRCVFVRDIGLRADEHGVDQALRFGRTTPAAFREVDYPWQERDLRTAP
                     RLYLHANDRIDADTWLRRRGLADAPLVLLQPGNWHVRRWWERRGTDPKFWPVAAWASL
                     LRAMHETLPAANLLLCGSPVESPVCEAIARETNLPQVKLATTDFPVRRLIAVLERAHS
                     MVSVDTGPAHLAAAMDCPLVVLYAGKYGPEKWDRRSPGGKPVVNLGVNPRVAAVADIA
                     PTQAIEAWRTLSASG"
BASE COUNT         1217 a         2564 c         2501 g         1469 t
ORIGIN      
        1 gtcggctcgc ggcccatttc ctgcaacatg gtgcggctga tgcggttgag cttgttgatc
       61 gtctctatca tgtgcaccgg gatgcggatg gtgcgcgcct ggtcggcgat cgagcgcgtg
      121 atcgcctggc ggatccacca cgtcgcgtag gtgctgaact tgtagccgcg gcggtattcg
      181 aacttgtcga ccgccttcat caagccgatg ttgccttcct ggatcaggtc gaggaactgc
      241 aagccgcgat tcgtatactt tttcgcaata gagatgacca ggcgcaggtt cgcttcgacc
      301 atttcctttt tcgcgcgccg cgccttggcc tcgccgatcg acatggcgcg gttgatttcc
      361 ttgatgtcgg tgagcgacag gcacaaggtc ttctccagcg cgatgagctt ttcctgctcg
      421 gcgacgatgt cggccttgtg cgcgcggatc gacgaggccc atttctgctt cttgcggatc
      481 acctcgtccg cgaaattcag gttcacctca taagcggtga aggccttgag gaaatccttg
      541 cgcggcatct tcgcctgcct gacgcagatg tccatgatca cgcgctcgtg gccacggatc
      601 gtcgccacga cctcgcgcaa cttgcgcacg aacgcatcga tcatgatcga cggcagcttg
      661 agcttgagga aagcctcggc catcgtgtca cgcgctttct gcgtcttctt gtcgccgtcg
      721 ccgtactttt cggcggcctt ctggaacttg gcgtagagcg agcgcaactc gtccatgcgc
      781 cgcgtcacct cggccggatc cggcccggtt tcgccgggac cggcttcttc ggtttcgccg
      841 ttctctttct cttcttcttc ggcttcggcc tgggcgggtt cggcttcgag catcgcatcg
      901 tccatgcccg gcacttcgat gtctgcaaag ccggtgagca cctcggacag gcgcttcttg
      961 ccttccagat gctggtcgta ttcctcgacc agcaacttga tcgaccaggg gaacgtggcc
     1021 aacgccgtct gcacctggcc caggccctct tcgatgcgct tggcgatggc gatttcgccc
     1081 tcgcgcgtga gcagttcgac cgtacccatc tcgcgcatgt acatgcgcac gggatcggtg
     1141 gtgcggccga cttcggcatc gaccgcgctg agcacggcca cggcttcctc ggtggcggtt
     1201 tcatcgtcgg tggcgaccgg gttttccggc agcagggtct cggtatcggg cgcgatttcg
     1261 tgcacctcga tgcccatgtc gttgatcatg ccgatgatgt cttcgatctg ttccggatcg
     1321 acgatgtcgt cgggcaggtg atcgttgacc tcggcgtagg tcaggtagcc ctgttccttg
     1381 cccttgatga tcagggtctt gatttcggat tgctgcggat tggtgttttc gatggccatg
     1441 ggaggcgtgc tgtatgtgtg attacaagac ttgaaagtat aggaggcgtg tcaaacgctt
     1501 aagtctccag ccagaacgtg accggtccgt cgttgatcag gctcacctgc atatgggcgc
     1561 cgaaacggcc gatttccact gtggaatggc gatttttcgc gatttcgacc aggcgttcga
     1621 accagcgccg gccggcatcc ggcgtggcgg cggcggtaaa gctcgggcgc atgcccgaag
     1681 cggtatccgc cgccagggtg aactgcggca ccagtagcaa gccgccgccg gtatcggcca
     1741 gggagcggtt cattttcccg ttcgtgtcgg cgaacacgcg gtagccgagc aagcgttcgc
     1801 acagccgctg cgcctgcgtc tcgccgtcgt ccggctgtac cgcgacgagc gccagcaatc
     1861 cgggaccgat gcgcgcgatc gtcgcgccgt ccacgtccac ggatgccgtg gtgacgcgct
     1921 ggatcagggc gatcatcgat ccatcgcctg ccggtacaat cgcgtttttc cgattcctgc
     1981 gtcttgatgc gttggcatgt cgccaccttg tatttcctgc tacgcctgac cggccgcctg
     2041 ccgctgcggg cgctgcacgg catcggcgcc ggactgggct ggctgctgtg gcgcctgccg
     2101 aacccgttgc gacgcaaggc cgtgcagacc ctgtcccatg taacaacgca aatcggcggc
     2161 aaaaacagtc acgccctgct caagcccgcg ctgatcgaaa ccggcaaagg cgcgctggaa
     2221 ctatgcaaga tctggtccgg cgatccgcag gccgcgctcg gactgatccg cgaaatccgc
     2281 ggcaccgagc atttcgacgc agccctggct gccggcaagg gactcctcat tgccgcaccg
     2341 cacctgggct gctgggaact gctgaatttc tggctgtgca gtcgcactcc catcgccatc
     2401 gcctaccggc cgccgcgccg cgccgagatc gagccgctgc tgatccgcgc gcgtggaggg
     2461 ctcgccgccg agcaggtgcg tgcggaaggc gccgccggcg tgcgcgcgct gttcaagcga
     2521 ttgagtgccg gcggcgtgat cggcatcctg cccgaccagc aacccaaggc cggcgaaggc
     2581 cagttcgcgc cgttcttcgg tacaccggcg ttgacgatgg tgctgctctc gcgcctcgcc
     2641 cagcgcaccg gcgcgaccgt gctgttcgcc ttcgccgaac gcctgcccaa aggcgccggt
     2701 ttccgcatcc atgtcctgcc cgcaccggaa ggcatcgccg acgccgacct gcccaccgcc
     2761 atcgccgcac tcaatcgccg cgtcgaggct tgcgtgtgca ttgcgccaga acaataccaa
     2821 tgggcgtaca agcggtattc gattcgtccg ccgcaggaac acggctgatt caggcgccag
     2881 atttttccaa ccgtcgcagg aacacctgca tttccttttc gacctgcttg tcgccgcgtt
     2941 tttgcgcggc ggcgatgccg ctacgataag tttcgatggc tgcggtcgca tcgccggatt
     3001 cggccaacag tttgccgagc tgtttccagg ccgccgaata gtccggatcg aattccagcg
     3061 cgcggcgcaa ttcggcgatc gcctcaaccg ccattccttc ggcgaccaag gcattggcca
     3121 gggccacgcg cagcagcgcg ccgtcgcgag gcccgctgca caaggcgcgc aggctgtcga
     3181 gccgcgcgct catgggcgcc cgcgatggat gtcgtgggtg gccacgccga tgctcgacgg
     3241 cggcggcacg aaccagtcgg ggtgcgcgag gctggcgcgc atatccgcca aacccgccgc
     3301 ccagtgttcg cgcatcgtgg ccgccccgaa cgcgtaatcc ttgtactgct cctcgtaggg
     3361 tttggcgcta tagatcaggt gcacgacgtt caaacacgcc tcgccgagcc agggtgcgag
     3421 ttcgcgcgcg agcggcgcgg gcaggcgctt gcccggcaac gcgtgcgcga gcttgcgcaa
     3481 tgccgtgcgc aaatgctggc gttcggcgag acggtcggtg ccatgccggg tgcggctgga
     3541 atactggatg tccttctgcc gttcgaacac atcggcgaga ttgcgcggca gctcgccgcg
     3601 cgcgctccac aggtccacct gcagggccag catgtcgcct tgcggttcgc tgtcgatcac
     3661 gtattcgagc ggcgtgttcg agaccacgcc gccgtcccaa taaaactcgc cgtcgatctc
     3721 gaccgcggga aatcccggcg gaagcgcgcc cgaggccatc acgtgctcgg cgcggatttt
     3781 cgtggcgcgg ttgtcgaaat agatgaaatt gccgctgcgc aggttgaccg caccgaccgc
     3841 caggtgcggc gccgtgcggt gattgatgcg gtcgaaatcg acgagttcct cgagcgtacg
     3901 cttgagcgga gcggtgctgt agatgctcgc cgtcgccgga tcgtgcgcat tccacagcgt
     3961 ggccggcggg cgcggcacga agaagccgct ctgcccgaac gcgagcgcat ccagcgcact
     4021 ggcggcgttg gcaaggctgc gccagggctc ggccgcggcg aacgcggaca tccactggcg
     4081 gcgccatccc gctgtgcgcc gcgccagcgg tagcgccggc gtggagatgc gttcccagaa
     4141 ttcgcgcaac cgatcaacgc gatgctgcgg cgggttgccg gcaagtagcg ccgcgttgat
     4201 cgcgccgacg gaaatgccgg cgaaccagtt cggcgcgatg ccggcttcgt gcaacccctc
     4261 gtagacgccg cactggtagg cgccgagcgc cccgccgccc tgcagaacga gggcgacgtg
     4321 ggcgtagtcg cgcacggccg cagctggcgt cttggtggac ttgcgacgtt gcggattggt
     4381 ggattttttc gcgccgcgcg cgcgcgccgg gctcactgca tgaaccagcc gtggctgacg
     4441 acgatgctct gtccggtcag cgcgttggtt tcgaagccgg cgaagaacag cgcgcaggcg
     4501 gcgacgtcgt cgaccgtggt gaattcgccg tcgacggtgt ccttgagcat cactttcttg
     4561 atcacatcgt cttcggaaat gcgcagctcc ttcgcctgtt ccggaatctg cttttccacc
     4621 agcggcgtgc gcacgaagcc ggggcagatc acgttcgcgc gcacgccatg cgcggcgcct
     4681 tccttggcca cgacttttgc cagaccgatc agaccgtgct tggcggtgac gtagggcgcc
     4741 ttgagcttgg aagcttcctt ggaatgcacc gagcccatgt agatgacgct accgcccttg
     4801 cccgccgcgt acatgcgctt caggcaggca cgcgtggtca ggaaggcacc gtcgaggtgg
     4861 atcgcgagca gtttcttcca atccgcgaac gggaattcct cgatcggata tacgatctgg
     4921 atgccggcgt tggaaaccag gatgtcgacg ccgcccaaat ccttgacggt tttcgccacg
     4981 ccagcctcga cctgctcctc gttcgtcaca tccatcaaca cggcactggc cttgacgccg
     5041 cgggttcgga tttctgccgc ggctgcgtcg gcggcggcct ggttgatgtc ggcgatggcg
     5101 atgtgcgcgc cattgtcggc gagcacttcg gcgatgcgct tgccgatgcc gctggcggcg
     5161 ccggtgacga gggcgacctt gttattcacg ctcatggttt tctcccgggg ttggtgttca
     5221 gtcctcgcgc accgcgtttt tcacatccac ccccaggtcg cgcagcttgc gatacaaatg
     5281 tgtgcgttcc atgccggccg ccttggccag tttgccgacg ctgccgccgg cctgttgcaa
     5341 ttgatgcagc agataggcgc gttcgaactg ttcgcgcgcc tcgcgcagcg gcaaatcgaa
     5401 atcgatgccg gctgtactcg gcgcaaccgc agacgcgacc gcgccgagcg cggcttcgac
     5461 ttcggccggt tccacatccg cggcaccgcc catcaccagc aagcgctgca cgaggttgcg
     5521 caactcgcgc acattgcccg gccaggcgta ggcgcacaga cgtttttgcg ccgcgtgcgc
     5581 aaagcggcga cagtgaagcc gatcgcggcc ggcgaagaaa tccgcgtaca gatgcagcag
     5641 ttccggaacg tcttcggcac gctcgcgcag cggcggcacg gacaaaggta ggacattgag
     5701 caggtagtag agttcctcgc ggaacgcacc ggccttgacc tgcgcgtcca gatcacgcgc
     5761 gctcgcggcg atcacgcgca aatccagcgg cgccggttcc cggccgccgc cgcgcaccag
     5821 ggcgcggcgc tgcaacacgc tgaacaggcg ttgttgcaac tccgcgtcga ggtcggcgac
     5881 ttcgtcgagg aacagcgtgc cgccgttggc ctgttcaagc aggccgggtt gcacgccgct
     5941 cgcatcctcg gtgccgaaca gcgcggcgac gagatgatcg cgcgggatgc tgccggccgc
     6001 gacggcgacg aacggaccgt tgcggcgcgg gccgtgcgcg tgcagccagc gcgcgagggt
     6061 ttccttgccg gtgccggctt cgccgcggat cagcacgctg gcatcgtgct gggcgagttt
     6121 ttgcagttgc gcgcgcagcg ccgccatcgg cttgctcgat cccaccggct ccagcggcaa
     6181 ggccaggcgc tgacgcaggc cttcgttttc gtgacgcagg cggctggctt cgcgtgcgcg
     6241 ctcgagcgtg agcagcagtt tcgccagtga aatgggcttc tcgatgaaat cccaggcgcc
     6301 caggcgcgtg gcttcgaccg cggtctccac cgtgccatgg ccggacatca tcaccacggg
     6361 gcaagccgcg agcacacccg ccgccgccca ttcgcgcaac aaggcgatgc cgtcggtacc
     6421 gggcatccag atgtcgagca ggatcgcgtc gggacgctcg cgttcgcgcg ctagacgcgc
     6481 ctcgtccgcg ttcgcggcga ggatggcgac atagccctcg tcctcgagga tttccttgac
     6541 ggtgctgcgg atatccggtt cgtcgtcgac gatgagtacg cgtccggcgc tcatggaacg
     6601 gcctgtggcg aaaatgcgac ggtcaagaat agcgcgtttc tccgcttcgg aagaaattaa
     6661 tccggcgccg ctagaatgcg cacgcccgtt tcagcccgca catccatggc agcatcgaca
     6721 acgcccctgc tcatccgttt cggccgcctc ggcgacatgc tgatgcagga accgttgctg
     6781 catctgctgc gccggcgcta cggtgcgcca tgccggattc tcacgcgcgg aacctggact
     6841 ggcgcgttgt acgccgcaca cccggacatc ggcgaaatcc ggcaattgca ctctcgtcac
     6901 gcaccgctcg cgtttagccc ggagcgctgg cgcgcgatcg ccgcgctgcg cgcgcatgaa
     6961 ggcccggtct acgtatccga ggataccgac cggtccctga tgcgcatcgg ctggatgctg
     7021 cgccttgcgc gcgtgccgcg cgaccggtgc gtgttcgtgc gcgacatcgg cctgcgcgcg
     7081 gacgagcatg gggtcgacca ggcgctgcgt ttcggtcgca ccacgcccgc cgcgttccgc
     7141 gaggtcgact atccctggca ggaacgcgac ctgcgcaccg cgccgcgcct gtacctccat
     7201 gcaaacgatc gcatcgatgc cgatacctgg ttgcgtcggc gcggcctcgc tgacgcgccg
     7261 ctggtcctgt tgcaacccgg caactggcat gtgcgtcgct ggtgggaacg acgcggcacg
     7321 gatccgaaat tctggccggt cgccgcatgg gccagcctgc tgcgcgccat gcacgaaact
     7381 ctgccggcgg cgaacttgct gctgtgtgga tcacccgtgg aatcgccggt atgcgaagcc
     7441 atcgcccgcg aaacgaattt gccccaagtg aaactggcaa cgaccgactt tcccgtgcgc
     7501 cgcctgatcg ccgtgctcga acgtgcacac agcatggttt cggtggacac gggaccggca
     7561 catcttgccg cggccatgga ctgcccgctg gtcgtgctct acgccggcaa atatggcccc
     7621 gagaaatggg accgtcgcag tccgggcggc aagccggtcg tcaaccttgg cgtcaatccg
     7681 cgtgtcgcgg cggtcgcaga cattgcgccg acgcaagcaa tcgaagcctg gcgcactcta
     7741 tcagccagcg g
//
LOCUS       DASRVO010000284         1326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_163989, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000284 DASRVO010000000
VERSION     DASRVO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163989"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..173
                     /locus_tag="VFF05_03495"
     CDS             18..173
                     /locus_tag="VFF05_03495"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50899.1"
                     /translation="MPPQSENDFVEIAASLIARVELPATTLYRLAGVGLSNFRDADEF
                     TQPELFN"
     gene            502..>1326
                     /locus_tag="VFF05_03500"
     CDS             502..>1326
                     /locus_tag="VFF05_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50900.1"
                     /translation="MASNLNCTANDVEVASVDVLNGVTTCVAGDPVTLHLQANIHANA
                     QRRYNVGIFMAQDGKTGLLTSANGGSAACSTFGLPIQTSPPSPFANLDGNVCGDFDNS
                     VLPSQPPPYPLDLGTVTVTCRPDASGYLDIPTVVTWMTNANTAGCAAPPAAYFVPESK
                     SKCEAGISQRIEVQVFGKIVVTKATNPAGAAASFSYTASGPSASPTSFSLSDGQSQTV
                     VTAALGATAQNYVISEQTLAGWTPGAQIVCKDIDGDIAPFVDVNNATGTITAHMSVS"
BASE COUNT          243 a          460 c          411 g          212 t
ORIGIN      
        1 gcagccacac gccggcgctg ccgccgcagt cggagaacga tttcgtggag atcgccgcgt
       61 cgctgatcgc gcgcgtggaa ctgccggcga cgacgcttta ccggctggcc ggcgtgggac
      121 tgtcgaattt ccgcgacgcg gacgaattca cccagccgga gctgttcaac tagcgggagt
      181 acgccggcac cgcgcccaac acggtgcatg cccgcaccca gcgccgcaac gacgaatgca
      241 cgtcggcacg cgcggagttg atttggatca acaatcgacg ccgtgcaccg tccgatcatc
      301 gccgccatcg cggggagatc cgtcatgggc gactcagaac tggccaccaa aggtgcgtcc
      361 aaaccaaggt ccggtaccga aggatcggaa cggcgcggga ttcaccgttt gagcattcct
      421 ggcctcgtcc tgcttgctgg cttctgcggg actgccttcg ccgcggcgcc ctatcctgtc
      481 tcgatgtgcc ctgcggaccg cgtggcctcg aacctcaact gcacggcgaa cgacgtcgaa
      541 gtcgcaagcg tggacgtcct caacggcgtc acgacctgcg tcgctggcga tccggtcacc
      601 ctgcacctgc aggccaatat tcatgcgaac gcccagagac gctacaacgt cggcatcttc
      661 atggcgcagg acggcaagac cggcctgctg acatccgcga acggcggcag cgccgcctgc
      721 tcgacattcg gcctgccgat ccagacgtcg ccgccatcgc cgttcgcgaa tctcgacggc
      781 aatgtctgcg gcgatttcga caatagcgtg ctgccgtcgc agccgccgcc gtatccgctc
      841 gacctcggca cagtcacggt cacctgcagg ccggacgcga gcggctacct ggacatcccg
      901 acggtcgtca cctggatgac gaatgcgaac acggcgggtt gcgcggcacc gccggcggcg
      961 tatttcgtac cggaaagcaa gtccaagtgc gaagcgggca tttcgcagcg catcgaagtg
     1021 caggtcttcg gcaagatcgt cgtgaccaag gcgacgaatc ccgccggcgc cgccgcatcg
     1081 ttttcctaca ccgcgagcgg gccctccgct tcgccgacca gcttttcgct gtccgacggt
     1141 caatcacaga cggtcgtgac cgctgcgctc ggcgcgaccg cgcagaacta tgtcatcagc
     1201 gagcagacgc tcgcgggttg gacgcccggc gcgcagatcg tctgcaagga catcgacggc
     1261 gacatcgcgc cgttcgtcga cgtgaacaac gcgaccggca cgatcaccgc acacatgagc
     1321 gtgtcg
//
LOCUS       DASRVO010000285         5040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_60651, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000285 DASRVO010000000
VERSION     DASRVO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60651"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFF05_03505"
     CDS             <1..689
                     /locus_tag="VFF05_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZW50901.1"
                     /translation="LIVALSLIAIIYVGYVALVQDDMKKLIAYSSVAHMGFVTLGLFI
                     AFALMRISGNTQAAYLGVQGSMVQMISHGFVSGAMFTCVGVLYDRMHTRMIRDYGGVV
                     NTMPWFAAFWVLFSMANCGLPGTSGFVGEFMVILASFAMNPWVAAGAAFTLIIGAAYT
                     LYLVKRVIWGDVTNDHVAKLKDINPREALVLGLFAAGVLILGVWPEPLTHLMDASVKH
                     IVDQLLVVKI"
     gene            703..2157
                     /gene="nuoN"
                     /locus_tag="VFF05_03510"
     CDS             703..2157
                     /gene="nuoN"
                     /locus_tag="VFF05_03510"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZW50902.1"
                     /translation="MNFNLHDLVLLAPEDFLLGATCAILFVDLFLKQSQRALTHWLSI
                     AALLGTIAIILTNHDPAARAFNGAYIHDTVAAVLKTFILGIVATVFVFARGYLRDRKL
                     FIGEFYTLMLFSTLGMMLLVSAGNLVMVYLGLELFALSSYALVAIHRDSGVASEAAMK
                     YFVLGALASGLLLYGLSMIYGATGTLDLAQIRQVGGVGVNGLSQHPTLFAFGLVFVVV
                     GIAFKFGAAPFHMWLPDVYEGSPTAVTIFIGSAPKLAAFGMAYRLLDSGLGDLAPHWS
                     QMLAALAALSLVIGNLVAIAQTNIKRMLAYSTISHMGFLLLGLLNATPSGYAAAMFYA
                     ICYAIMTTAAFGVILLLARTGFEAEMIDDFKGLNQRNPWYAFIMLLGMFSLAGVPPLW
                     GFFAKLLVLKAAIDAGFLWLAIVAIVMAIVGCFYYLRVVKVMYFDAPADKSALALPQD
                     LPLRWLLSINGVALLVLGVLWGPLIGWCYRAFGL"
     gene            2207..2283
                     /locus_tag="VFF05_03515"
     tRNA            2207..2283
                     /locus_tag="VFF05_03515"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2241..2243,aa:Met,seq:cat)
     gene            2394..2897
                     /gene="rimP"
                     /locus_tag="VFF05_03520"
     CDS             2394..2897
                     /gene="rimP"
                     /locus_tag="VFF05_03520"
                     /inference="COORDINATES: protein motif:HMM:NF000927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZW50903.1"
                     /translation="MFNKELTQLLTPVAADLGLECLGVEYSPSHGNGLVRVYIDAPER
                     AVTVDDCEAVSRQVSALLDVNDPIQGRYTLEVSSPGLDRPLYTPEQFARVKGNAAKIE
                     VNLAIAGRRRFQGTILAVEGEDVVVEQDGTPVRIAHANIHKAKLVPQYDEPAGKPGKH
                     KQAKKSK"
     gene            2908..4401
                     /gene="nusA"
                     /locus_tag="VFF05_03525"
     CDS             2908..4401
                     /gene="nusA"
                     /locus_tag="VFF05_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZW50904.1"
                     /translation="MSKDLLLVVDAVANEKGVDKNVIFEAMEAALASAAKKRYPNEDV
                     ATRVSIDQQSGDYETFRRWEVVADDVVMESPDRQLRLMDAVDTRADIQVGEFIEEPID
                     NPDFGRIAAQAAKQVIVQRVREAERAQVVDAYKDRVGELLTGVVKRVERGSIFLDLGG
                     NAEAYIPRDKAVPREAVRAGDRVRGYLFEVRPEPRGPQLFVSRTAPEFMMELFKLEVP
                     EVGQGLVEIKGAARDPGDRAKIAVLAHDNRTDPIGACIGMRGSRVQAVSNELNGERID
                     IVLWNDNPAQFVINAMAPAEVQSIIMDEEKHSMDIAVAEDKLSQAIGRGGQNVRLASK
                     LTGWQLNVMTQDQVAQKSEAEQEAARKLFQDKLEVDAEIAGILVQEGFTTVEEIAYVP
                     TGELLAIEGFDENIVEELRARARDALLTEALAVEEDIEDNKPAEDLLGVEGMDETTAF
                     ALAEHAVVTRENLADLATDELMEFAIDGMDEDRAAALIMAARAGQTA"
     gene            4451..>5040
                     /locus_tag="VFF05_03530"
     CDS             4451..>5040
                     /locus_tag="VFF05_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HZW50905.1"
                     /translation="MSDVTIKQLAGVLGLSVDKLLVQLDGAGMKFSGPDQIITSAEKL
                     KLHGFLRRAHGKDDKPADEGSAPRQITLKRKSVSELTVNQGAARGKTVAVEVRQKRTY
                     VKRSAVAESQEVDLEREDALKKLQESQARNQAEQAALKAQDKRRAEEEAKLRAAEEAR
                     RADEARQRAEVEAKTGEPAAPAEKPAKEERAAAAPR"
BASE COUNT          919 a         1549 c         1639 g          933 t
ORIGIN      
        1 ggctgatcgt ggcgctgtcg ctgattgcga tcatctacgt cggctacgtc gccctcgtgc
       61 aggacgacat gaagaagctg atcgcgtatt cgtcggtcgc gcacatgggc ttcgtcaccc
      121 tgggcctgtt cattgccttc gccctgatgc gcatttcggg aaacacgcag gccgcgtatc
      181 tcggcgtgca aggctccatg gtgcagatga tctcgcacgg cttcgtctcg ggtgcgatgt
      241 tcacctgcgt cggtgtgctc tatgaccgca tgcacacgcg catgatccgc gattacggcg
      301 gcgtggtgaa cacgatgccg tggttcgccg cgttctgggt acttttctcg atggccaact
      361 gcggcctgcc ggggacgagc ggcttcgtcg gcgaattcat ggtgatcctg gccagctttg
      421 cgatgaatcc ctgggtcgcc gccggcgccg cattcacgct catcatcggc gcggcctaca
      481 cgctgtatct ggtcaagcgc gtgatctggg gcgatgtgac caacgaccac gttgccaagt
      541 tgaaggatat caacccgcgc gaagcgttgg tgctcggcct gtttgccgcc ggcgtgctca
      601 tcctcggcgt ctggccggaa ccgctcacgc acttgatgga cgcgtcggtc aagcacatcg
      661 tcgatcaatt gctggtcgtg aagatttgag gaaagattgc cgatgaactt caacctccac
      721 gatctcgtcc tgctcgcgcc cgaggatttc ctgctcggtg cgacctgcgc gatcctattc
      781 gttgacctgt tccttaaaca gtcgcagcgt gcgctcacgc attggttgtc gattgccgcg
      841 ctgctcggca ccatcgcgat catccttacc aatcacgatc cggcggcgag ggcgttcaac
      901 ggcgcctaca tccacgacac ggtcgcggcc gtgctcaaga cgttcatcct cggcatcgtc
      961 gccacggtat tcgtgttcgc gcgcggctac ctgcgcgacc gcaagctatt catcggcgag
     1021 ttctacacgc tgatgctgtt ctcgaccctg ggcatgatgt tgctggtgtc ggcgggcaac
     1081 ctcgtcatgg tgtacctggg gctggaactg tttgcgctgt cgagctatgc gctggttgcc
     1141 atccaccgcg attcgggcgt ggcttccgag gcggcgatga agtatttcgt gcttggcgct
     1201 ctcgcttcgg ggcttttgct ttacggcctg tcgatgatct acggcgcgac cggcacgctg
     1261 gatttggcgc agatccgcca ggtcggcggc gtcggcgtga atggactttc ccaacatccg
     1321 accctgttcg cgttcggcct cgtgttcgtg gttgtcggca tcgcgttcaa gttcggcgcc
     1381 gcgccgttcc acatgtggct gccggacgtc tacgagggtt cgcccacggc ggtgacgatc
     1441 ttcattggtt ccgctcccaa gctcgccgcg ttcggcatgg cctaccgcct gctcgacagc
     1501 ggcctcggcg acctcgcacc gcactggagt cagatgctcg ccgcgctcgc cgcgctgtcg
     1561 ctggtcatcg gcaatctcgt cgccatcgcg cagacgaaca tcaagcgcat gttggcgtat
     1621 tcgacgattt cgcacatggg ctttctgctg ctcggcctgt tgaacgcgac cccatcgggt
     1681 tacgcggcgg cgatgttcta cgcgatctgc tacgcgatca tgaccaccgc cgccttcggc
     1741 gtgatcctgc tgctcgcgcg caccggcttc gaggccgaaa tgatcgacga cttcaagggc
     1801 ctgaaccagc gcaatccgtg gtacgccttc atcatgttgc tgggaatgtt ctcgctggcc
     1861 ggtgtcccgc cgctgtgggg attcttcgcc aagctgctcg tgctcaaggc cgccatcgac
     1921 gctggcttcc tgtggctggc gatcgtcgcc atcgtcatgg ccatcgtcgg ctgtttctac
     1981 tacctgcgcg tggtcaaggt gatgtacttc gacgcgccgg cggacaagtc cgcgctcgcg
     2041 ctgccgcagg atctgccgct gcgctggctg ttgtcgatca atggcgtggc cctgctcgtg
     2101 ctcggcgtgc tgtgggggcc gctgattggc tggtgctacc gtgccttcgg actgtgacgc
     2161 ggttgcggaa atctcccgca ctcggctaga atgcgcgttc ccctggtgcg gggtggagca
     2221 gtctggcagc tcgtcgggct cataacccga aggtcgcagg ttcaaatcct gcccccgcta
     2281 ccaacgtttt acttttaccc atgcgcagga tgcgcgttgc cgatgtttgg atgcgagaca
     2341 acggcggacc aggaaaggcc ctcgtggcct ttttttgtac ccgcgataaa cccatgttca
     2401 acaaggaatt gacgcaattg ttgacgccgg tggcggctga tctcggcctg gaatgcctcg
     2461 gcgtcgaata cagcccgtcg catggcaacg gactggtgcg cgtgtacatc gacgcgccgg
     2521 agcgcgcggt gacggtggac gattgcgagg cggtgagccg gcaggtttcg gcgctgctcg
     2581 acgtgaacga tccgattcag ggccgttaca cgctggaagt gtcgtcgccg gggctggatc
     2641 gccctttgta tacgccggag cagttcgcgc gcgtcaaggg caacgcggcg aagatcgaag
     2701 tgaacctggc gattgcggga cggcgccggt tccaggggac gattctcgcg gtggaaggcg
     2761 aggatgtggt tgtggaacag gatggaacgc cggtgcgcat cgcgcacgcg aacatccaca
     2821 aggcgaagct cgttccgcag tacgacgaac cggccgggaa accgggcaaa cacaagcaag
     2881 cgaaaaaatc gaagtgagag caagaatatg agcaaggatc ttctgttggt cgtcgatgcc
     2941 gtcgccaatg aaaagggcgt cgacaagaac gtcatcttcg aagcgatgga agcggcgctc
     3001 gcttccgcgg cgaagaaacg ctatccgaac gaggacgtcg ccacgcgcgt gtccatcgac
     3061 cagcaaagcg gcgactacga aaccttccgc cgttgggaag tggtggccga cgacgtcgtc
     3121 atggaatcgc cggatcggca actgcgcctg atggacgcgg tcgatacgcg cgcggacatc
     3181 caggtcggcg agttcatcga ggaaccgatc gacaatcccg atttcggacg catcgccgcg
     3241 caggccgcca agcaggtgat cgtgcagcgc gtgcgcgaag ccgaacgcgc gcaggtggtc
     3301 gatgcctaca aggatcgcgt cggcgaattg ctcaccggcg tggtcaagcg cgtcgagcgc
     3361 ggcagcattt ttctcgacct gggcggcaat gccgaggcct acatcccgcg cgacaaggcg
     3421 gtgccgcgcg aagcggtgcg tgccggcgac cgcgtgcgcg gttacctttt cgaagtgcgt
     3481 ccggaaccgc gcggcccgca gttgttcgtc tcgcgcaccg cgccggaatt catgatggag
     3541 ctgttcaagc tcgaagtgcc ggaagtcggc cagggcctgg tcgaaatcaa gggcgccgcg
     3601 cgcgatccgg gcgatcgcgc caagatcgcc gtgctcgcgc acgacaaccg caccgatccg
     3661 atcggcgcct gcatcggcat gcgcggttcg cgcgtgcagg cggtgtccaa cgaattgaac
     3721 ggcgagcgca tcgacatcgt gctgtggaac gacaatccgg cgcagttcgt gatcaatgcg
     3781 atggcgccgg ccgaggtgca gtcgatcatc atggatgagg aaaagcactc gatggatatc
     3841 gcggtcgccg aggacaagct ctcgcaggcg atcgggcgcg gcggccagaa cgtgcgcctt
     3901 gccagcaagc tcaccggctg gcagctcaac gtgatgaccc aggatcaggt cgcgcagaaa
     3961 tccgaggccg aacaggaagc cgcgcgcaag ctgttccagg acaaactcga ggtcgacgcg
     4021 gaaattgccg gcatcctcgt gcaggaaggc ttcaccacgg tcgaggaaat cgcctacgtg
     4081 ccgaccggcg aactgcttgc gatcgaaggc ttcgacgaga acatcgtcga ggagttgcgc
     4141 gcgcgtgccc gcgatgcgtt gctcaccgag gcgctcgcgg tcgaagagga catcgaggac
     4201 aacaagcccg ccgaggatct gttgggcgtc gaaggcatgg acgaaaccac cgcgttcgcg
     4261 ctggccgagc acgccgtcgt cacgcgtgag aatctcgccg atctcgccac cgacgaattg
     4321 atggaatttg cgatcgacgg catggacgag gatcgcgctg ccgccttgat catggcagcg
     4381 cgtgccggac agacggcctg agtccgtacg caacgacctc gcgcgatacg ctcacggaga
     4441 accaaacatc atgtcggacg taaccatcaa gcaactcgcc ggtgtactcg gcttgagcgt
     4501 ggacaagctg ctcgttcagc tcgacggagc cggcatgaag ttctccggtc ccgaccagat
     4561 catcaccagc gcagagaaac tcaagctgca cggattcctg cgtcgcgcgc acggcaagga
     4621 cgacaagccc gccgatgaag gctcggcgcc gcgccagatc acgctcaagc gcaaatcggt
     4681 cagcgagctg accgtgaacc agggcgccgc gcgcggcaag accgtggccg tggaagtgcg
     4741 gcagaaacgt acttacgtca agcgcagcgc cgtcgccgag tcgcaggaag tcgatctcga
     4801 acgcgaggac gcgctgaaga aattgcagga gtcgcaggct cggaaccagg ccgagcaggc
     4861 cgcgctcaag gcccaggaca agcgccgcgc cgaggaagaa gccaaattgc gcgcagccga
     4921 agaagcgcgt cgtgccgacg aagcgcggca gcgcgcggaa gtcgaggcga agaccggcga
     4981 accggcggcg ccggcggaga agcccgcgaa ggaagaacgc gccgcggcag cgccacgcaa
//
LOCUS       DASRVO010000286         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_242223, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000286 DASRVO010000000
VERSION     DASRVO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242223"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1110)
                     /gene="trpS"
                     /locus_tag="VFF05_03535"
     CDS             complement(76..1110)
                     /gene="trpS"
                     /locus_tag="VFF05_03535"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZW50906.1"
                     /translation="MAFKERVFSGVQPTGNLHLGNYLGAITKFVALQERYDCIYCVVD
                     MHAITVWQDPSELARATREVTAAFLACGIDPKQHIIFNQSQVAEHAELAWVFNCVARM
                     GWLNRMTQFKEKAGKDRENVSVGLFVYPNLMAADILLYCATHVPVGEDQKQHLELSRD
                     IAQKFNNDFAASIHGHGFGDAFFPLPEPIIQGPATRVMSLRDGSKKMSKSEPSDYSRI
                     NLTDDADAIAQKIRKAKTDPEPLPSEEKGLATRPEADNLVGIYAALNEVSKAQVLSEF
                     GGAQFSAFKAALVDLAVAKLGPIGAEMKRLVQDPVYIDEILADGSARAQAIAADTMKA
                     VKDIVGFVRR"
BASE COUNT          195 a          397 c          371 g          220 t
ORIGIN      
        1 gggccgccgc ccttcgaggt tcggcgcttc gcgccgaaca cctctgggtg acggcgcaag
       61 gccatgcgcc agcgattacc gccgcacgaa accgacgatg tccttgaccg ccttcatggt
      121 gtcggcggcg atggcctgcg cgcgcgccga accgtcggcg aggatttcgt cgatataaac
      181 cgggtcctgc accagccgct tcatttcggc gccgatcggc ccgagcttgg cgaccgcgag
      241 atcgaccagc gccgccttga aggcggagaa ttgcgcgccg ccgaactcgc tcagcacctg
      301 cgccttgctc acctcgttga gcgccgcata gatgccgacc agattgtcgg cctccggccg
      361 cgtggccagg cccttctcct cgctcggcag cggctccgga tcggtcttgg ccttgcggat
      421 cttctgcgcg atagcgtcgg cgtcgtcggt cagattgatg cgcgaataat ccgacggctc
      481 cgacttcgac atcttcttgg agccgtcgcg caggctcatc acgcgcgtcg ccggcccctg
      541 gatgatcggc tccggcagcg gaaagaacgc gtcgccaaag ccgtggccgt ggatcgaggc
      601 ggcaaaatcg ttgttgaact tctgcgcgat gtcgcgcgac agctccaagt gctgcttctg
      661 gtcctcgccc accggcacat gcgtggcgca atagagcaga atgtcggccg ccatcaggtt
      721 gggatagacg aacagaccga ccgaaacatt ctcgcggtcc ttgccggcct tctccttgaa
      781 ctgggtcatg cggttgagcc agcccatgcg cgcgacgcag ttgaacaccc aggcaagctc
      841 ggcgtgctcg gccacctggc tctggttgaa gatgatgtgc tgcttgggat cgatgccgca
      901 ggccaggaac gccgcggtca cctcgcgcgt ggcgcgggcc aattcgctcg ggtcctgcca
      961 caccgtgatg gcgtgcatgt cgaccacgca atagatgcag tcatagcgtt cctgcagcgc
     1021 cacgaatttg gtgatggcgc cgagataatt gccgaggtgc aggttgccgg tcggctgcac
     1081 gccggaaaac acgcgctcct tgaacgccat tgctcattcc tcttggcttg acgcgccgtc
     1141 ccatgccacg ccgggccggc cttgggcaag gccctcacaa ccc
//
LOCUS       DASRVO010000287         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_138236, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000287 DASRVO010000000
VERSION     DASRVO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138236"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFF05_03540"
     CDS             complement(<1..265)
                     /locus_tag="VFF05_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50907.1"
                     /translation="MRLFVQGLFVSACLAGSPCGAMADDHFARDPQQPVDQAYTAKIA
                     KYTTQTDFNSPLTDYLPASNTVPTPEIVLGDVAGAPNMLPYAED"
     gene            527..>1130
                     /locus_tag="VFF05_03545"
     CDS             527..>1130
                     /locus_tag="VFF05_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HZW50908.1"
                     /translation="MINITLPDGSKRRFDNPVTVADVAASIGAGLAKAALAGKVDDKL
                     VDTSFRIDHDANLAIITEKSPEALDILRHSTAHLLAQATQRLFPDTQVTIGPVVENGF
                     YYDFARKTPFTPEDLDKIEAEMKKIVAESLPVQRRVMPKDDAIKFFKDKGEVYKAQII
                     DEIIPPGEEISLYGQGDWVDLCRGPHVPNTNKLRAFKLMKV"
BASE COUNT          237 a          345 c          361 g          187 t
ORIGIN      
        1 cgtcttccgc gtacggcagc atgttcggtg cgccggcaac atcgccaagc actatttccg
       61 gcgtcggcac cgtgttcgat gccggcaggt agtcggtcag cggcgagttg aagtcggtct
      121 gcgtcgtgta cttggcgatc ttcgccgtat acgcctgatc gaccggctgt tgcggatcgc
      181 gggcgaaatg gtcgtcggcc atcgcgccgc agggcgagcc ggccagacaa gcgcttacaa
      241 acaatccctg cacgaaaaga cgcatgtgcg gctccgaaag cgtggacacg atggttcgcg
      301 agcatagcgg ccagccggat gcgtgtcatg gccgaaggtc atggtgcgat tgaaagcgcc
      361 ggcgcggcgt gggaaaatca cttggaactg tgcagaggtg gcgcgcgggc aaccgccgtc
      421 gagcgtgcga catgtcaact aggactgccg acaagtaacc gcgacgcaca cgcatttctc
      481 gaggaagggg cgcggtgcgc ccttttttat tgtttggaat ccagccatga tcaacatcac
      541 tctccccgac ggcagcaaac gccgattcga caatccggtg acggtggccg acgtcgccgc
      601 ctccatcggc gcaggactcg ccaaggccgc gctggccggc aaggtcgacg acaaactggt
      661 cgacacgagt tttcgcatcg accacgacgc gaacctcgcc atcatcacgg aaaaaagtcc
      721 cgaggcgctg gacatcctgc gccattccac cgcgcacctg cttgcgcagg cgacgcagcg
      781 gctgtttccc gacacccagg tgacgatcgg cccggtcgtc gagaacggtt tctactacga
      841 cttcgcgcgc aagacgccgt tcacgccgga agacctcgac aagatcgagg ccgagatgaa
      901 gaagatcgtc gcggaaagcc tgccggtgca gcgccgcgtg atgcccaagg acgacgcgat
      961 caagttcttc aaggacaagg gcgaggtcta caaggcacag atcatcgacg agatcattcc
     1021 gcccggcgag gaaatctcgc tgtacgggca gggcgattgg gtggacctgt gccgcggccc
     1081 gcacgtgccg aacacgaaca agctgcgcgc gttcaagctg atgaaggtgg
//
LOCUS       DASRVO010000288         1238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_216909, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000288 DASRVO010000000
VERSION     DASRVO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216909"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFF05_03550"
     CDS             complement(<1..714)
                     /locus_tag="VFF05_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50909.1"
                     /translation="MVSPDCAALMASRSVQVAAQTLEPSPPAGSPMALTVHIVAAHAG
                     ATNAKQAAAHSIDIPKRRRAMTRMVVLPCHCEGEAALSYIQGGFFCASHPDAHCYNAP
                     HFPEVSLRMRLSRFHLATVKEVPADAEIASHRLMLRAGMLRKHAAGLYTWSPLGLKVL
                     KKVEAMVREEMNRAGALEITMPTVQPRELWDETGRWQKFGPQLLKIKDRKEAEYCYAP
                     TAEEVVTDFARSELKSYKQL"
     gene            716..>1238
                     /locus_tag="VFF05_03555"
     CDS             716..>1238
                     /locus_tag="VFF05_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50910.1"
                     /translation="MFAPVLDGQTIRLSLYTNCLTTSDTLDSSTNAAGNPCLPPATWP
                     GYVTQFGPSAFFISGKSLTIDAAAGLSHGIVLDASAANAGCGPGSCFRFFDVDGGSAL
                     TLRGLTLRGGAGGGSALGAGGAIFNQGVLTVERCTLVGNVARGGSNGGLAGSGVGQDG
                     IGGLGGAGPNGGGA"
BASE COUNT          187 a          413 c          413 g          225 t
ORIGIN      
        1 caactgcttg tagcttttca gctcgctgcg cgcgaaatcg gtgacgactt cctcggcagt
       61 cggcgcgtag cagtattcgg cttccttgcg atccttgatc ttgagcaact gcggaccgaa
      121 tttctgccag cgcccggtct cgtcccacaa ctcgcgcggc tgcaccgtcg gcatggtgat
      181 ctcgagcgcg ccggcgcggt tcatttcctc gcgcaccatg gcctcgacct tcttcagcac
      241 cttcaggccc agcggcgacc aggtgtaaag cccggcggcg tgcttgcgca gcatgcccgc
      301 gcgcagcatc agccggtggc tggcgatctc cgcgtcggcg gggacttcct tgacggtggc
      361 gagatggaac cgggacaggc gcatgcgcag cgatacctca ggaaaatggg gcgcattgta
      421 acagtgcgca tccgggtgcg aggcgcaaaa gaatccgcct tggatatagg atagggccgc
      481 ttcgccttcg caatgacagg ggaggaccac catgcgcgtc atcgctctgc gccgcttcgg
      541 gatgtcgata ctgtgcgctg ccgcctgctt ggcgttcgtt gcgcccgcgt gggccgcaac
      601 aatgtggacg gtcaacgcca tcggcgaacc ggccggcggg ctgggctcca gcgtttgcgc
      661 tgccacctgc acgctgcgcg acgccatcaa tgccgcgcaa tcgggcgaca ccatcatgtt
      721 cgctcccgta ctcgatgggc agaccatccg cctgtcgctc tacaccaact gcctgaccac
      781 cagtgatacg ctggacagtt ccaccaacgc cgcaggcaat ccctgcctgc cgccggcgac
      841 ctggcccggc tacgtcacgc aattcggccc ctcggcgttc ttcatcagcg gtaaatcgct
      901 gaccatcgac gcggctgccg gtttgagcca tggcatcgtg ctcgatgcca gcgcggcgaa
      961 tgccggatgc gggcccggat cgtgtttccg cttcttcgac gtcgatggcg gcagcgcgct
     1021 cacgctgcgc ggattgacgt tgcgcggtgg cgccggcggc ggcagcgcgc tgggtgcggg
     1081 cggcgccatc ttcaaccaag gcgtgctgac cgtggaacgc tgcacgctgg tcggcaatgt
     1141 ggcgcgtggc ggctcgaacg gtggcttggc tggcagcggc gtggggcagg acggcatcgg
     1201 cggcctcggc ggcgccggtc ccaacggcgg cggcgctg
//
LOCUS       DASRVO010000289         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_191306, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000289 DASRVO010000000
VERSION     DASRVO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191306"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1059)
                     /locus_tag="VFF05_03560"
     CDS             complement(<1..>1059)
                     /locus_tag="VFF05_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HZW50911.1"
                     /translation="KEIKRLDQALTKLRADLDRMLERGDVADGGEHRDVLEAYRMFAN
                     DHGWSHKLHEAVATGLTAEAAVERVQSDTRARMLRSTDPYLRDRLHDLEDLGHRLMRQ
                     LVGQDHAPSREQLPDNAILIARAMGPAALLDYDRKRLRGLVLEEGTANSHVSIVARAL
                     GIPAVGEVPNAPGIADPGDAIIVDGTSGEIYVRPSAEIESAYAERVRFRARRQAQYSA
                     LRDRPCVTRDGQPIELMINAGLTIDLPHIDDTGSAGIGLFRTELQFMVGQNLPRSSDQ
                     LALYRTVLDAAGPKPVTFRTLDIGGDKALPYMETVVEENPALGWRAIRLGLDRPGLLR
                     GQIRALLRAGGGRALRIM"
BASE COUNT          156 a          347 c          370 g          186 t
ORIGIN      
        1 aacatgatgc gcagcgcgcg gccgccgccg gcgcgcaaca gcgcgcggat ctggccgcgt
       61 aatagcccgg gccggtcgag accgagccgg atcgcgcgcc agccgagcgc gggattttcc
      121 tcgaccacgg tttccatata gggcagcgcc ttgtcgccgc cgatgtcgag cgtccggaag
      181 gtcaccggct tcgggcctgc cgcgtccagc accgtgcggt acagcgcgag ttggtcgctg
      241 ctgcgcggca gattctggcc gaccatgaac tgcagttcgg tgcggaacag gccgattccg
      301 gcactgccgg tgtcgtcgat gtggggcagg tcgatggtga ggcccgcatt gatcatcagt
      361 tcgatcggct ggccgtctct ggtcacgcag ggcctgtcgc gcagcgcaga atattgcgcc
      421 tggcgtcggg cgcgaaaccg cacccgttcc gcgtaagccg attcgatttc cgccgacggg
      481 cgcacataga tctcgccgga ggtgccatcg acgataatgg cgtcgccggg atcggcgatg
      541 cctggtgcgt ttggcacttc gccgaccgcg gggatgccga gcgcgcgcgc cacgatcgag
      601 acgtgggaat tggcggtgcc ttcctccagc accagcccgc gcaacctctt gcgatcgtag
      661 tcgagcagcg ccgcgggccc catcgcgcgc gcgatcagaa tggcgttatc gggcagctgt
      721 tcccgcgacg gggcgtgatc ctgccctact aattgccgca tcagccgatg gccgagatct
      781 tcgaggtcgt ggaggcggtc gcgcaggtaa ggatcggtcg agcgcagcat gcgcgcacgg
      841 gtgtcggatt gcacgcgctc gaccgcggcc tccgcggtga ggccggtggc gaccgcctca
      901 tgcagcttgt gcgaccagcc gtgatcgttg gcgaacatcc ggtaggcttc cagcacgtcg
      961 cggtgttcac cgccgtcggc gacatcgccg cgctccagca tgcggtcgag gtcggcgcgc
     1021 agcttcgtca gcgcttggtc gagccgcttg atttccttc
//
LOCUS       DASRVO010000290         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_217000, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000290 DASRVO010000000
VERSION     DASRVO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217000"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..489)
                     /gene="acpS"
                     /locus_tag="VFF05_03565"
     CDS             complement(106..489)
                     /gene="acpS"
                     /locus_tag="VFF05_03565"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZW50912.1"
                     /translation="MIYGVGTDIVEIARVHKVLARYGERFAQRILCPPELTRFRAHRQ
                     PVAYLAKRFAAKEAFTKALGTGIHAPANWHGVWVVNLRSGQPKLEFTPALAALLAQRG
                     VGRCHLSLSDERAFASATVILESGA"
     gene            complement(486..1244)
                     /locus_tag="VFF05_03570"
     CDS             complement(486..1244)
                     /locus_tag="VFF05_03570"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZW50913.1"
                     /translation="MIELGVNIDHVATVRQARRTYEPDPVWAAVEAHLGGADGITAHL
                     REDRRHIQDEDVRRLRELTHIKLNLEMAATEEMVKIACSVKPEMAMLVPEGRHEITTE
                     GGLDVVSQESKLKDAIAPLAAAGIKVSVFIDAELRQVEAAARIGAVVCEIHTGPYAHA
                     FHSKGRDAESPAVVHELDKLKQAGEAVRGLGMRFNAGHALNYFNVEPVARLAGVRELH
                     IGHAIVSRAVFVGMREAVREMKRLIVEAARSPQR"
     gene            complement(1241..>1876)
                     /gene="recO"
                     /locus_tag="VFF05_03575"
     CDS             complement(1241..>1876)
                     /gene="recO"
                     /locus_tag="VFF05_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZW50914.1"
                     /translation="GRVALLARGARRPRSAMRGVLLSFHPLRLSWSASGELGTLIAVE
                     WAAGLRSLEGLGLMCGFYLNELLLRLLPREDPHEGLFDAYAGSLARLSAGSPHAAVLR
                     AFERRMLAELGYAPVLERDAETGLAIEAERRYAYEPDRGPVAVTGREGRPGAGTEVVV
                     SGRTLLDLARDEFERAETRDEARTLLRALIAHRLGGQTLHTRTVLMELQEL"
BASE COUNT          334 a          643 c          580 g          319 t
ORIGIN      
        1 tgaacagaat cacgccgccg gtggtgcgat ggcgcaggcg ggcgcgatct ccgtccgtga
       61 gcgtgcagcc gataacgtcg atgatcgccg gccccggcgc cacgctcatg cgccggactc
      121 cagaatcacg gttgcactgg cgaacgccct ctcatccgaa agggacaagt gacagcgccc
      181 cacgccgcgt tgcgccagaa gcgcggcgag cgcaggcgtg aactcgagct tgggttggcc
      241 ggaccgcagg ttgacgaccc atacgccgtg ccagttggca ggcgcgtgaa tgccggtgcc
      301 aagtgccttc gtgaatgcct ccttggccgc gaaacgcttg gcgagatagg cgacaggctg
      361 acggtgggcc cggaatcgcg tcaactccgg cggacacagg atccgctggg cgaagcgctc
      421 gccgtagcgc gcaagcacct tgtgtacgcg cgcgatctcc acgatgtccg ttccgacccc
      481 gtaaatcatc gttgaggcga gcgcgcggct tccacgatca ggcgcttcat ctcccgtacc
      541 gcctcgcgca taccgacgaa tacggcccgg cttacaatgg catgcccgat gtgcaattcg
      601 cgaacaccgg caagacgcgc gaccggttcc acgttgaaat agttcagtgc gtggcccgca
      661 ttgaaccgca tgcccaggcc gcggactgcc tctccggcct gcttgagttt gtctagttcg
      721 tgcaccaccg ccgggctctc ggcatcgcgg cccttggaat gaaacgcgtg cgcatagggg
      781 ccggtgtgta tttcgcaaac gaccgctcca atgcgagccg ccgcctcgac ctgccgaagt
      841 tctgcgtcga tgaaaactga cactttgatc ccggcggcag ccagaggcgc gatcgcgtcc
      901 ttcagcttgg attcctgcga gacgacatcc agcccgcctt ccgtagtgat ctcgtgccgc
      961 ccctcgggaa ccagcatggc catctcgggc tttacgctac aggcgatttt gaccatttct
     1021 tcggtcgccg ccatttccag attgagcttg atgtgcgtga gttcgcgcaa ccgccgcaca
     1081 tcctcatcct ggatatggcg ccgatcctcg cgcaggtgcg ccgtgatgcc gtcggcgccc
     1141 ccaaggtgcg cctcgacggc cgcccagacc ggatcaggct cgtaagtacg gcgcgcctgg
     1201 cgcacggtcg cgacatggtc gatattgacg ccaagttcga tcatagctcc tgcagctcca
     1261 tcagcaccgt gcgcgtgtga agcgtctggc ctccgaggcg atgcgcgatc agtgcacgca
     1321 acagcgtgcg agcctcgtcc cgcgtctcgg cgcgctcgaa ttcgtctcgc gcaaggtcga
     1381 gaagggtgcg gccgctgacc accacctcgg tgccggcgcc cgggcgccct tccctgcccg
     1441 tcactgcgac cggaccgcgg tccggttcgt aagcgtagcg ccgctccgcc tcgattgcca
     1501 ggccggtttc tgcgtcgcgc tcgagcacgg gagcataccc gagttccgcc agcatcctgc
     1561 gctcgaaggc tcgcaatacc gcggcatgcg gcgagcctgc ggacaggcgg gcaaggctgc
     1621 cagcgtaggc atcgaaaagc ccttcgtgtg gatcctcgcg cggcagcagt ctgagcagaa
     1681 gttcattcaa atagaatccg cacatcaggc caagaccctc gagcgagcgc aatcccgccg
     1741 cccattcgac agcaatcagc gttccgagct caccggaagc gctccagctc aatcgcagtg
     1801 gatgaaagga gagcagcacg ccgcgcatcg ccgagcgcgg acgccgtgct ccgcgcgcga
     1861 gcagcgcaac tcgacc
//
LOCUS       DASRVO010000291         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_138381, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000291 DASRVO010000000
VERSION     DASRVO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138381"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFF05_03580"
     CDS             <1..618
                     /locus_tag="VFF05_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50915.1"
                     /translation="DLRRRLREPDLRYAAPAALHERIAMSAQATRTSRRSPPAWLRYA
                     AACVIAFGAGGISMRLWHAAESGNALQEQVDRDLFAAHWRALAAASPMDVVSTDQHTV
                     KPWFAGKVPESPLVRDFAAQGFALAGGRIDYVGGGRVPVLVYRHGQHWIDVFVFASGT
                     PMPAAAQVQGYHLVPIQLGNQPAAVVTDMEATELSSLIRLLTTPA"
     gene            complement(658..894)
                     /locus_tag="VFF05_03585"
     CDS             complement(658..894)
                     /locus_tag="VFF05_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZW50916.1"
                     /translation="MNTWQVPKCADSGAGSLRGIVAIVAASGDTVDFSQLNSSNCPTD
                     QNLSNRVSGASIDIGAYELQQGDIVFNPNFEGCP"
     gene            complement(1046..1324)
                     /locus_tag="VFF05_03590"
     CDS             complement(1046..1324)
                     /locus_tag="VFF05_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50917.1"
                     /translation="MKTLARFALVATLTCGAAGAQSGGGYDLHWNAPAAGGATMADAG
                     YTLTGTVAQPAAKAACASGASGYTLRDGFWAGQPGGDVIFRNGFETGC"
     gene            complement(1321..>2396)
                     /locus_tag="VFF05_03595"
     CDS             complement(1321..>2396)
                     /locus_tag="VFF05_03595"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HZW50918.1"
                     /translation="VNTVAAGVFGATIGGGGFRGTSGNFTNSVSGVGGTVSGGSSNTA
                     GSGAIVGGGNANTANGVYSVVSGGQLNSATGGAAMVPGGQVNKAGGFASMAAGSYAHV
                     RSAAETGGAADDGTFVWSDSTGTNSNQFTSNGVNQFLIRAGGGVGINTIPYNPEAELT
                     IQGSATGASSNADIILLPRSSPYGFDLSVSGTAQGDAVFAIDTTANNFAKIFGLDASG
                     NLSIAGATASKPGGGSWSVPSDARLKRDIQPLENILDKLLQLRGVTYEYRSPDGLHPP
                     GRHTGFIAQQVQPIFPEWVGRTADGYLSVGPTGFEAMTVEALRELRAEQDGEIAQLQA
                     ENAQLHERLDDIAARLDRLEARP"
BASE COUNT          412 a          793 c          763 g          428 t
ORIGIN      
        1 gatttgcgtc gaagattgcg cgaaccggat ctgcgctacg ccgcgcccgc cgccctgcac
       61 gaacgcatcg ccatgtcggc gcaagcgacg cgaacttcgc gccgctcgcc gcccgcatgg
      121 ctgcgctatg ccgccgcctg cgtgatcgcc ttcggcgcgg gaggcatttc gatgcgtctg
      181 tggcatgccg ccgaatcggg caatgccctg caggaacagg tcgaccgcga tctcttcgcc
      241 gcgcactggc gcgcgctggc cgccgcttcg ccgatggacg tcgtttccac cgaccagcac
      301 acggtcaaac cctggttcgc cggcaaggtg ccggaatccc cgctcgtgcg cgacttcgcc
      361 gcgcagggct tcgcgctcgc cggcggccgc atcgattacg tcggcggcgg gcgcgtgccg
      421 gtgctggtct atcggcatgg ccagcactgg atcgacgtgt tcgtgtttgc gtccggcacg
      481 ccaatgcctg cggcagcgca ggtgcagggc taccacctcg taccgatcca actcggcaac
      541 caacccgccg ccgtcgtcac cgacatggag gcgacggaat tgagttcgct gatccgtctg
      601 ctgacgactc cggcttgaac agcatcagcg cgttggatcg ttcttgacta ctgacggcta
      661 aggacagcct tcgaaattgg gattgaagac gatgtcgccc tgctgcaact cgtaggcgcc
      721 gatgtcgatc gacgcaccgg aaacccggtt cgagaggttc tgatcggtcg ggcagttcga
      781 cgaattgagc tgcgagaaat cgacggtgtc accggaagcg gccacaatgg cgacgatgcc
      841 gcgcagactt ccggcgccgc tgtcggcgca cttgggaact tgccacgtat tcacggcgaa
      901 cgcatccggc gccgtcggcg aaaacaacac cgtgcagcag gcggcgggcg gcagacagga
      961 tggatgacgc tgaattcttc atggatttcg ctcccggggg tgtgcgcatc gaaaggaatg
     1021 cgcctcgaag caaaaccgaa caatctcagc accccgtctc gaaaccgttg cggaagatga
     1081 catcgccgcc tggctgcccg gcccagaagc cgtcgcgcaa ggtgtagccg gacgcgccgc
     1141 tggcgcaagc ggctttcgcg gcaggttgag ccacggtacc ggtgagcgtg tagccggcat
     1201 ctgccatcgt cgcgccacct gcagcaggcg cgttccagtg caagtcgtag ccaccgccgg
     1261 attgcgcacc cgctgcgccg caggtcagtg ttgccacgag tgcaaaacgc gcgagggtct
     1321 tcatggccgc gcttccagcc gatccagccg tgcggcgatg tcgtcgagac gctcgtgaag
     1381 ttgcgcgttc tcggcttgaa gctgtgcgat ctcgccgtct tgctcggcgc gcaattcgcg
     1441 cagggcttcc acggtcatcg cctcgaaacc ggtggggccg acactcaagt atccgtccgc
     1501 ggtgcgcccg acccactcgg gaaatatcgg ctgcacttgt tgcgcgataa agccggtgtg
     1561 gcggccgggc ggatgcaggc catccggact gcggtactcg taggtgacgc cgcgcagttg
     1621 cagcagcttg tcgagaatat tttccagcgg ctggatatcg cgcttcaggc gcgcatccga
     1681 tggcaccgac cacgagccgc cgccgggttt gctagctgtg gcacccgcga tcgagagatt
     1741 tccggaggca tccaggccga agatctttgc aaaattgttg gccgtggtat cgattgcgaa
     1801 caccgcgtca ccttgcgcgg tgccgctcac gctcaggtcg aacccgtagg gcgaactgcg
     1861 tggcagcaga atgatgtcgg cgttggaact ggcacccgtc gccgaaccct gtatggtcag
     1921 ctcagcctcc ggattgtacg gaatcgtatt gatgccaacg ccgccaccgg cgcgaatcag
     1981 gaactgattg acgccgttgg aagtgaactg gttgctgttc gtgccggtgg aatccgacca
     2041 gacaaaggtg ccgtcgtccg cagcgccgcc cgtttcggcg gcgctgcgca catgggcgta
     2101 ggatcccgcg gccatgctgg cgaagccacc ggccttgttg acctgcccgc ccggcaccat
     2161 tgcggcgccg ccggttgcgc tgttgagctg ccccccactc accacgctgt agacaccgtt
     2221 cgccgtattt gcgttgccgc cgccaacgat cgcgccgctg ccggccgtat tggaactgcc
     2281 gccgctaacc gtgccgccga cacccgatac cgagttggtg aagtttccgg acgttccacg
     2341 aaaaccgcca cctccgatcg tcgcgccgaa cacgccagcc gcaaccgtgt tcacgg
//
LOCUS       DASRVO010000292         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_269066, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000292 DASRVO010000000
VERSION     DASRVO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269066"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFF05_03600"
     CDS             complement(<1..283)
                     /locus_tag="VFF05_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50919.1"
                     /translation="MHKSIMAAAAAALLVSATAFAAADAIDNGNLLVKPAKNNTFATG
                     GYVLGKAELFGYIGELKDSKQITGIVLRDADRATPEQKHLLVVTAQAQKI"
     gene            complement(323..724)
                     /locus_tag="VFF05_03605"
     CDS             complement(323..724)
                     /locus_tag="VFF05_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50920.1"
                     /translation="MRKNSVIRGVQALFVVLLLALLAACGGRDAIKNGTSDIAAAGAP
                     TTFDLTVAADADGQFEYQGATLTREDLAGHIRYLAEIGKPVHSILLTTGEKQKIKNDH
                     VAALAGICRDLKVTGYVRDNDGNLKVIRIVK"
     gene            complement(740..1309)
                     /gene="dcd"
                     /locus_tag="VFF05_03610"
     CDS             complement(740..1309)
                     /gene="dcd"
                     /locus_tag="VFF05_03610"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZW50921.1"
                     /translation="MTIKSDHWIRRMAEKHGMIEPFEPGQVRTNANGGKLISYGTSSY
                     GYDVRCANEFKVFTNINSTIVDPKEFDARSFVDIKDEVCIIPPNSFALARTVEYFRIP
                     RSVLTICLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTPLPARIYANEGVAQMLF
                     FESDEVCGTSYRDRGGKYQGQQGVTLPKA"
     gene            complement(1334..2422)
                     /gene="apbC"
                     /locus_tag="VFF05_03615"
     CDS             complement(1334..2422)
                     /gene="apbC"
                     /locus_tag="VFF05_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HZW50922.1"
                     /translation="MTQPTKEQVERLLAEIIDPHTGQDLMASAAVKGVGVDGGKVAID
                     IVLGYPAKTFVDALAQQVRAKLAQAGIADAAVGVTSRVFAHKVQKDLTPMPNVKNIIA
                     VASGKGGVGKSTTAANLALALAAEGANVGVLDADIYGPSMPRMLGVSGKPDTKDGQRI
                     EPKLAHGIQAMSIGFMIDEDTPMIWRGPMVTQALTQLLNETNWVDLDYLVIDLPPGTG
                     DIQLTLCQRVPVSGAVIVTTPQDIALLDARKGLKMFEKVDVPVLGIVENMALHTCSNC
                     GHTEHIFGAGGGVRMAAQYGVPLLGSLPLDIRIREQGDGGTPTVAADPTGAVAALYRE
                     IARKTAAQLALHARSKAIAFPKIVIQNT"
     gene            2659..3327
                     /locus_tag="VFF05_03620"
     CDS             2659..3327
                     /locus_tag="VFF05_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HZW50923.1"
                     /translation="MLQGRPQPSVYKDDIVDAAGGPLTSALDGVDLIKSHQLPGGHED
                     IVYLVRDGRNATVSFLYMKFLFGNHRFSEPRELRDALQYIDVDEGSWARHVQAGLALS
                     KERRVLFLKYEDLTRRPEWFLRQLLEFAGTPLPPCVLDECVRLEKQSDAYMRNPYNGF
                     MYRPQPGSIYELLMRHRTGDYWRYLFDAASKRYFHDQGGTQFLLHFGYEESPDWWQRP
                     SLPR"
     gene            3438..>3845
                     /locus_tag="VFF05_03625"
     CDS             3438..>3845
                     /locus_tag="VFF05_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HZW50924.1"
                     /translation="MRIAAIVLLGFVAHASARDASPVGTWQTFDDDTHAPKALVRIED
                     HAGMVSGRIVKLFPAQGDDPDPRCADCRGERRDRPVLGMTILWNFRRDGDGWVGGEIL
                     DPESGDIYRATLHPRDDGATLVVHGYIGIPLLGR"
BASE COUNT          655 a         1225 c         1206 g          759 t
ORIGIN      
        1 cgatcttctg cgcctgcgcg gtcaccacga gcagatgctt ctgctccggc gtggcgcgat
       61 cggcatcacg caacacgatg ccggtgatct gcttgctgtc cttcagctcg ccgatgtagc
      121 cgaacagctc ggccttgccc aggacgtagc cgccagtcgc gaacgtgttg ttcttggccg
      181 gcttgaccag caggttaccg ttgtcgatgg catcggcggc ggcaaacgca gtcgcggaaa
      241 cgagcaacgc tgcggcagcc gccgccatga tcgatttgtg catcgtattc tcccgttgcg
      301 tggcgacgcc gcgcgggcgt cgttacttga cgatccgaat gactttcagg ttcccgtcgt
      361 tgtcgcgcac gtacccggtc accttgaggt cgcggcaaat acccgccagt gcggccacat
      421 gatcgttctt gattttctgc ttctcgccgg tggtcagcag gatgctgtga accggcttgc
      481 cgatttcggc gagatagcgg atgtgcccgg cgaggtcttc gcgggtgagc gtggcgccct
      541 ggtattcgaa ctggccatcc gcgtccgcgg ccacggtcag gtcgaacgtc gtcggcgcac
      601 ccgcggcggc gatatccgaa gtgccgttct tgatcgcatc gcggccaccg caggcggcaa
      661 gcaaagcgag caggagcacg acgaacagcg cttgcacgcc gcgaatgacg gaatttttgc
      721 gcattgtata ctcctcaact caagctttcg gcagggtcac gccctgctgg ccctggtatt
      781 tgccgccacg gtcacggtaa ctggttccgc aaacctcgtc ggactcgaaa aacagcattt
      841 gcgccacgcc ttcgttcgcg taaatccgcg ccggcaacgg cgtggtgttg gaaaattcca
      901 gcgtcacgtg gccttcccac tccggctcca gcggagtcac gttgacgatg atgccgcagc
      961 gcgcgtaggt gctcttgccg aggcatatcg tcagcacgct gcgcggaatg cgaaagtact
     1021 ccaccgtacg cgccagcgca aacgaattgg gcgggatgat gcacacctcg tccttgatgt
     1081 cgacgaaact gcgtgcgtcg aattccttgg gatcgacgat cgtcgagttg atgttcgtga
     1141 acaccttgaa ctcgttcgcg cagcgcacgt cgtaaccgta gctcgaggtg ccgtaggaaa
     1201 tgagtttgcc gccgttggca ttggtgcgca cctggcccgg ctcgaacggc tcgatcatgc
     1261 cgtgcttctc cgccatgcgg cggatccagt ggtccgactt gatggtcatg gattgatgat
     1321 cacgggttga tgctcatgtg ttctggatga cgatcttggg aaacgcaatc gccttgctgc
     1381 gtgcatgcag ggcgagctgg gccgcggtct tgcgcgcgat ctcgcgatac agcgcggcga
     1441 ccgcaccggt cggatcggcc gcgaccgtcg gcgtgccgcc atcgccttgc tcgcggatac
     1501 ggatgtcgag cggcagcgat ccgagcaatg gcacgccgta ctgcgcggcc atgcgcacac
     1561 cgccgcccgc gccgaagatg tgctcggtgt ggccgcagtt cgaacacgtg tgcagggcca
     1621 tgttctcgac gatgccgagc accggcacgt cgaccttctc gaacatcttc aggcccttgc
     1681 gcgcatccag cagggcgatg tcctgcggcg tggtgacgat caccgcgccg gataccggca
     1741 cgcgctggca caacgtcaac tggatgtcgc cggtgccggg cggcaggtcg atgacgaggt
     1801 aatccaggtc cacccaattg gtttcgttga gcagctgcgt cagcgcctgc gtcaccatcg
     1861 ggccgcgcca gatcatcggc gtgtcctcgt cgatcatgaa gccgatcgac atggcctgga
     1921 tgccgtgcgc gagcttgggt tcgatgcgct ggccgtcctt cgtgtccggc ttgccggaga
     1981 cgccgagcat gcgcggcatg gatggtccgt agatgtccgc atcgagcacg ccgacgtttg
     2041 cgccttcggc ggcgagtgcg agggcgagat tcgccgcggt cgtggacttg cccacgccgc
     2101 ccttgcccga ggccaccgcg atgatgttct tcacgttcgg catcggcgtc aggtctttct
     2161 gcaccttgtg cgcgaacacg cgcgaggtca cgccaaccgc cgcatcggcg atgccggcct
     2221 gcgccagttt cgcgcgcact tgctgcgcca acgcatcgac gaaggttttc gccggatagc
     2281 cgagcacgat gtcgatggcg accttgccgc catccacgcc cacgcccttg accgctgcgg
     2341 acgccatgag gtcctggccg gtatgcgggt cgatgatctc ggcgagcagg cgctcgacct
     2401 gctctttcgt gggttgggtc atggcgggag tcgtgcgaaa acgattgtcc attgtagccg
     2461 agcgcgcgct gtgccgcatg cgcacgcgtg cgtgcgcgca cggcaggaaa cgcgatttgc
     2521 acggtgacgg tgcgctcgat aagctcggcg gattgtcctt gtatcgcgcg caggcacccg
     2581 ctgttgccct cccgaaagcg aattttcctt gtctcctatc cgcgctcggg cagcaccttg
     2641 ttgcgcaagt actttgcctt gctgcaagga cgcccgcagc caagcgtgta caaagacgac
     2701 atcgtcgatg cagccggcgg cccgttgacg agcgccctgg atggcgtcga cctgatcaag
     2761 agtcaccagc tgccgggtgg gcacgaggac atcgtttacc tggtgcgcga cggaagaaac
     2821 gccaccgtat cgtttctcta catgaaattc ctgttcggca atcaccggtt ttccgaacca
     2881 cgggaattgc gcgatgcgtt gcaatacatc gatgtggacg aaggttcctg ggcgcgccat
     2941 gtgcaggcag gcctggcgct gagcaaagag cggcgcgtgt tgttcctgaa atacgaagac
     3001 ctcacccggc gaccggaatg gttcctgcgg cagttgctgg aattcgccgg cacgccattg
     3061 ccgccatgcg tactcgacga atgcgtgcgc ctggaaaagc aatccgatgc ctacatgagg
     3121 aatccgtaca acggcttcat gtaccggccg cagccaggtt ccatctacga gctgctcatg
     3181 cgccatcgca ccggggatta ctggcgctac ctgttcgatg cggcttcaaa acgctatttc
     3241 cacgatcagg gcgggacaca attcctgctg cactttggct acgaagaatc ccccgactgg
     3301 tggcaacgac cgtcattgcc gagatgacac gcgccgcagt tccgcgccgc gacgatctct
     3361 ttcggcccgt atgccggtgg tcgccaccca tcaccagcgc ccactgaaac atttgttcga
     3421 tcgcttatga tggcggcatg cgcatcgccg ccatcgtcct gctcggattc gtcgcgcacg
     3481 cgtccgcgcg cgacgcctcg ccagtcggca cctggcaaac tttcgacgac gacacacacg
     3541 cgcccaaggc gctggtgcgg atcgaggatc atgccggcat ggtctctggc cgcatcgtca
     3601 aactgtttcc ggcgcaaggc gacgatccgg atccgcgttg cgccgattgc cgcggcgagc
     3661 gccgcgatcg accggtgctc ggcatgacga tactgtggaa tttccgccgc gacggcgacg
     3721 gctgggtcgg cggcgagata ctcgacccgg aaagcggcga catttaccgc gcgaccctgc
     3781 atccgcgcga cgatggcgcc acgctcgtcg tgcatggcta catcggcatt cccctgctcg
     3841 ggcgc
//
LOCUS       DASRVO010000293         1715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_242454, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000293 DASRVO010000000
VERSION     DASRVO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242454"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VFF05_03630"
     CDS             complement(<1..664)
                     /locus_tag="VFF05_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50925.1"
                     /translation="MARLAVITHTYDNFRGRKPLLGSLAAHWAGAGHSIEVVEGLGDW
                     PDADAAILHVDLSVVPEAYHAAAKRYPIVLNGNATDIRKTRVSRNLLKRGDDWTGPVV
                     VKTDLNFRGIPERFALECFRRDGKTPDLAPVPLVSTDKPYPILRSIAEVPDEVWNNPG
                     LVVERFLPERDAQGFWMRAWVFFGGRERCTRYCSTHPIVKTANIIAREPAPVPDELRA
                     ERE"
     gene            complement(713..1633)
                     /locus_tag="VFF05_03635"
     CDS             complement(713..1633)
                     /locus_tag="VFF05_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HZW50926.1"
                     /translation="MNAAYSIGTPGLVWSDAEKTQWLSRQKKRRSYADEVLGRIEALR
                     SRFDVREYGRLDYAPETFPLFAIRNRDWNQALPVALVTGGVHGYETSGVQGALQFVDR
                     HLADYAGRINVLVAPCVSPWAYERIHRWNPQALDPNRSFRENGPAPESAALMKLVAPL
                     RDRFILHIDLHETTNSDESEFRPALAARDGKPFEPEAIPDGFYLCADAANPQPEFQQA
                     VIAAVAKVTHIAPADAKGEIIGSPVVAPGVIEYDYARLGLCPRITNARYKTTTEVYPD
                     SPRATPEQCNAAQVAAVRAALDFILAQVPG"
BASE COUNT          292 a          550 c          560 g          313 t
ORIGIN      
        1 gttcgcgttc ggcacgcagc tcgtccggca ccggcgccgg ctcgcgcgca atgatgttcg
       61 cggttttgac gatcggatgc gtgctgcaat agcgcgtgca acgctcgcgt ccgccgaaaa
      121 acacccacgc gcgcatccag aaaccctgcg catcgcgctc cggcaagaag cgctcgacca
      181 cgaggccggg attgttccag acttcgtccg gcacctcggc aatcgaacgc aggatcggat
      241 acggcttgtc cgtactgacg agcggcaccg gcgccaggtc cggcgtcttg ccgtcgcggc
      301 ggaagcattc cagcgcgaaa cgctccggaa tgccgcggaa attcagatcc gtcttgacga
      361 cgaccgggcc cgtccagtcg tcgccgcgct tgagcagatt gcggctcacg cgggttttgc
      421 ggatgtcggt ggcgttgccg ttgagcacga tcggatagcg tttggcggcc gcgtgatagg
      481 cttccggaac gaccgacaaa tcgacatgca ggatggcggc gtcggcatcc ggccagtcgc
      541 ccagtccctc gaccacttca atgctgtggc ctgcgccggc ccaatgcgcg gccaggctgc
      601 cgagcagcgg tttgcgccca cgaaaattgt cgtaggtgtg agtaattacg gcaaggcgtg
      661 ccacagcgtc cccggtcgac gcgacgaagg ccgcgtcaac gcggatgata cttcaacccg
      721 ggacttgcgc cagaatgaaa tccagtgccg ctcgcactgc cgcaacctgc gcggcgttgc
      781 attgctccgg cgtcgcgcgc ggactgtccg gatacacctc ggtcgtggtc ttgtagcgcg
      841 cgttcgtgat gcgcggacac aatccaagtc gtgcgtagtc gtactcgatc acgccgggcg
      901 cgacgacggg cgaaccgatg atttcgccct tcgcatcggc cggcgcgatg tgcgtgacct
      961 tcgcgaccgc cgcgatcacc gcttgctgga actccggctg cggattcgcc gcatcggcgc
     1021 acaggtagaa cccgtccggg attgcttccg gctcgaacgg cttgccgtcg cgcgcggcca
     1081 gcgcgggccg gaattcggat tcgtcgctgt tggtggtttc gtgcaggtcg atgtgcaaga
     1141 tgaagcgatc gcgcagcggc gcgacaagtt tcatgagtgc cgccgattcc ggcgccgggc
     1201 cgttttcgcg gaacgagcgg ttcggatcga gcgcctgcgg attccagcga tggatgcgct
     1261 cgtaggccca cgggctcacg cacggcgcga ccaacacgtt gatgcggccg gcatagtcgg
     1321 ccaggtggcg gtcgacgaat tgcagcgcgc cctgcacgcc gctggtttcg tagccatgca
     1381 cgccgccggt gaccagtgcc acgggcaatg cctggttcca gtcgcgattg cggatcgcga
     1441 acagcgggaa tgtttcgggc gcatagtcga ggcgaccgta ctcgcgcacg tcgaaacgcg
     1501 aacgcagcgc ctcgatcctg cctagcacct catcggcata gctgcgccgc ttcttctggc
     1561 gcgacagcca ctgagttttt tcggcgtcgc tccaaaccag gcctggagtg ccgatggagt
     1621 atgctgcatt cattgcgttg cccgtcgagc gtctggagcg aagcgttcac tgtagcattg
     1681 cgcgatgcct cagcgaaaaa catgacgcag ccatt
//
LOCUS       DASRVO010000294         1451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_9124, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000294 DASRVO010000000
VERSION     DASRVO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9124"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VFF05_03640"
     CDS             complement(<1..443)
                     /locus_tag="VFF05_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW50927.1"
                     /translation="MTGAWLTRHQRSILLLILILAAGGIAAAWNLPVALFPQMDFPRV
                     VIAIDAGDRPIERMEAEITRPLERVLRGVPEVQRVRSTTSRGSAEIALTFPWGANMAQ
                     ALLQTQAAMNGALPSLPPGVSFFAQRRDPTVFPCLGLTLTSATRDP"
     gene            complement(440..>1451)
                     /locus_tag="VFF05_03645"
     CDS             complement(440..>1451)
                     /locus_tag="VFF05_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW50928.1"
                     /translation="ALASAPAAHADEDDAQPAVAAVASVRVAPATLAPMKISLTAYGT
                     ANFSADALQTISVPYQARVVSVAVVAGQAVRKGDALLVLAPTAAAALELQRAGNDAQF
                     ARKELARTRELFAQHLATNTDLAAAEQATHNSDAALASANARYDGTGERTLRATRDGV
                     VADIAMHAGEIIAADTAFAHVGDTSGLRLDLAVEPAAIAQVHPGEAVKFHLLQDRATE
                     QHASVERVGSQIDAQTRLIPVVAKLADSAQVPPGAAVSAQIETGSSEPVLGVPRSAVL
                     WRDGKAYVFVIQSGKATLRWVDAGADDGERIEIRSGLAVNESVVVLGNYELQDGMAVK
                     AQ"
BASE COUNT          233 a          468 c          517 g          233 t
ORIGIN      
        1 ggatcgcgcg tggccgaagt caacgtgagc ccaagacagg gaaacacggt cggatcgcgg
       61 cgctgggcga agaacgacac gcccggcggc agcgatggca gggcgccatt catcgctgcc
      121 tgcgtttgca gcagcgcctg cgccatgttc gcaccccacg ggaaagtcag cgcgatttcc
      181 gcggagccgc ggctggtcgt cgaacgcacg cgctgcactt cgggcacgcc gcgcagcaca
      241 cgctcgagcg gacgggtgat ttccgcctcc atgcgttcga tcgggcggtc gccggcatca
      301 atggcgatca ccacacgcgg aaaatccatt tgtggaaaaa gcgcgaccgg caggttccac
      361 gccgcggcga tgccgccagc ggcgaggatc aggatcagca gcaggatcga gcgctgatgc
      421 cgggtcagcc aggcgccggt cattgcgcct tgaccgccat gccgtcctgc aattcgtaat
      481 tgccgagcac gacgacggat tcgttcacgg cgaggcccga gcggatttcg atgcgctcgc
      541 cgtcgtccgc gccggcatcg acccagcgca gcgtggcctt gccggactgg atcacaaaga
      601 cgtaggcctt gccgtcgcgc cacagcacgg ccgagcgagg cacgccgagc accggttcgc
      661 tggagccggt ttcgatttgc gcgctcaccg ctgcgcctgg cggcacctgg gccgaatcgg
      721 cgagtttcgc gacgaccgga atcagtcgcg tctgcgcatc gatctggctg ccgacgcgtt
      781 cgacgctggc atgttgttcg gtggcgcggt cctgcaggag atgaaacttc acggcctcgc
      841 ctgggtgcac ctgtgcgatc gcggcaggtt cgacggcaag atccaggcgc agaccggatg
      901 tgtcgccaac atgcgcgaaa gccgtgtcgg cggcgatgat ttcgcccgca tgcatggcga
      961 tgtccgcgac cacgccgtcg cgcgtggcgc gcaaggtgcg ctcgccggtt ccatcgtagc
     1021 gggcgttggc ggaagccagt gcggcatccg aattgtgcgt ggcttgttcg gcggcggcga
     1081 ggtcggtgtt cgtcgccaga tgctgggcga acagctcgcg cgtgcgcgcc agttccttgc
     1141 gcgcgaactg cgcgtcgttg ccggcgcgtt gcaattccag tgcagcggcg gcagtcggcg
     1201 cgagcacgag caaggcatcg cccttgcgca cggcctgccc ggcgacgacc gcgacgctga
     1261 ccacgcgcgc ctggtacggc accgatatgg tttgcagagc gtcggcggaa aaattcgccg
     1321 tgccgtaggc agtgagggat attttcatgg gcgcgagcgt ggccggtgcg acgcgcacgc
     1381 ttgccacggc agccaccgcc ggctgtgcgt cgtcttcgtc ggcgtgggcg gcaggcgcgg
     1441 aggcaagggc a
//
LOCUS       DASRVO010000295         1383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_35643, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000295 DASRVO010000000
VERSION     DASRVO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35643"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFF05_03650"
     CDS             <1..211
                     /locus_tag="VFF05_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50929.1"
                     /translation="RDYTVVDFSSLPQLSGRHVRLITVGGKRIEGFLASADDHHLQLR
                     IERGGGSALIAIDRSGISQVQLLHW"
     gene            252..824
                     /locus_tag="VFF05_03655"
     CDS             252..824
                     /locus_tag="VFF05_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZW50930.1"
                     /translation="MNDKPATGFAQDIDAMILARAQRGDMAAFEEIYRRYGRACFQLA
                     LRLTGQRQTAEDLVHEAFLKMMDTIASYRGEAPFGAWFKRLTANAGIDWLRRQRHVAD
                     TGAEASFEQIAAAEADPAGLVDADSLLAHLPPRARTIVVLHELEGYTHAELAGLFGQS
                     ESYSKSILARALKRLHALAAPAASTSRVAL"
     gene            821..1291
                     /locus_tag="VFF05_03660"
     CDS             821..1291
                     /locus_tag="VFF05_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50931.1"
                     /translation="MSEQELNFRDLAARLPASATAHVPPADLWPRIVATHLNRRRQRR
                     WRRLAAGGGALVLVAALAITAGVWRHAATPTIDWQARAQALELQLDALPTPAASNPLA
                     QLAESELAHLDGNLQAAYDRGAAPRDLDPLWQRRSELLDALLSVRRQQVLLSRI"
BASE COUNT          234 a          474 c          430 g          245 t
ORIGIN      
        1 aagggattac acggtcgtcg atttttccag cctgccgcaa ttgtccggcc gccatgtccg
       61 cctgatcacg gtcggtggca aacggatcga aggattcctc gcatccgccg acgaccatca
      121 cctgcaactg cgcatcgaac gcggcggcgg cagtgcactg atcgcgatcg accgcagcgg
      181 catatcccag gtgcaactgt tgcactggta gcgactgcga cgattcttcc gcccacttcg
      241 tctaggctgc ggtgaacgac aaaccggcca cgggattcgc ccaggacatc gacgcgatga
      301 tcctggcacg cgcgcagcgt ggagacatgg cggcgttcga ggaaatctac cggcgctacg
      361 gccgcgcctg tttccagttg gcgctgcgcc tgaccgggca gcgccagaca gccgaggacc
      421 tggtacacga ggcatttctg aaaatgatgg acaccatcgc cagctatcgc ggcgaagcgc
      481 cgttcggcgc atggttcaaa cgccttaccg cgaacgccgg catcgactgg ctgcgccgcc
      541 agcgccacgt ggccgatacc ggtgcggaag catcgttcga gcagatcgcc gccgccgagg
      601 ccgacccggc cggcctcgtc gatgccgact ccctgcttgc gcacctgccg ccgcgcgcac
      661 gcacgatcgt ggtgctgcac gaactggaag gctacacgca cgccgagctt gcggggcttt
      721 tcggacagag cgaaagttat tccaaatcga tcctggcgcg cgcgctcaag cgtctgcacg
      781 cgctggccgc tcctgcggca tccacatcac gggtggcttt atgagcgaac aggaactgaa
      841 ttttcgcgat cttgccgcac gtttgccggc atccgcgacg gcgcatgttc cgccggcgga
      901 tttgtggccg cgcatcgtcg ctacgcatct gaatcgccgc cggcagcgcc gctggcgtcg
      961 gctggcggca ggcggcggcg cactcgtatt ggtcgccgcg ctggcaataa cagccggcgt
     1021 ctggcggcat gcggccacgc ccacgatcga ctggcaggca cgcgcacagg cgttggagct
     1081 gcaactggat gccctgccga cgccggccgc aagcaatccg ctcgcgcaat tggccgagtc
     1141 cgaacttgcg cacctcgacg gcaacctgca ggccgcctac gaccgcggcg cggcgccgcg
     1201 cgatctcgac ccgttgtggc aacgtcgcag cgaattgctc gacgccttgc tgtcggtgcg
     1261 ccggcaacaa gtccttcttt cccggatctg agatgtcatc atgaatccac gcctcgtttt
     1321 cgcttccatt ctgctgttgt gcgcgacgac ggccacggcc gcttcgccgt cttcttcgct
     1381 agg
//
LOCUS       DASRVO010000296         1140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_164274, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000296 DASRVO010000000
VERSION     DASRVO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164274"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /gene="xseA"
                     /locus_tag="VFF05_03665"
     CDS             complement(<1..820)
                     /gene="xseA"
                     /locus_tag="VFF05_03665"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZW50932.1"
                     /translation="MAEHSAPALTPRIWQVGALCRAIADALEARFNPVAVRGEVSGFS
                     RAASGHCYFSLKDAQGQLRCAMFRRAAALMEFMPRDGELVEVQGRLAVYEPRGDLQLI
                     VETLARAGAGTLFEQFLQLKARLEAQGLFDPARKRPLPAFPRAIGIVTSTGAAALHDV
                     VTCLRRRAPHVPVVLAAAAVQGPQAPGELVRALFSLYRLAEAGRVDLILLVRGGGSIE
                     DLWAFNEEVVARAIAASKIPIITGIGHEVDVSIADLVADYHAHTPTEAAQVAVAY"
     gene            809..988
                     /locus_tag="VFF05_03670"
     CDS             809..988
                     /locus_tag="VFF05_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50933.1"
                     /translation="MLGHQENRGLRAGCALNLVHSAWKPRRHWGDSPENRDFAKQAFD
                     FTGFFIAQIVIDVSN"
BASE COUNT          210 a          382 c          366 g          182 t
ORIGIN      
        1 aataggcaac cgccacctgg gcggcctccg tgggcgtgtg ggcgtgatag tcggccacca
       61 ggtcggcaat gctgacatcc acctcgtggc cgatgcccgt aatgatgggg atctttgatg
      121 cggcgatcgc gcgggcgacg acctcctcgt tgaaggccca caggtcctcg atcgagccgc
      181 cgccgcgcac cagcaggatc aggtccaccc ggccggcttc ggccaatcgg tagagcgaaa
      241 acagtgcccg cacgagctcg cccggcgcct gcggtccctg cactgcggcg gcagcgagca
      301 cgaccggcac atgcggcgcg cgccgccgca agcacgtgac cacgtcgtgc agcgctgccg
      361 caccggtcga agtcacgatg ccgatcgccc gggggaaagc cggcagcggc cgctttcgtg
      421 ccggatcgaa caggccttgc gcctccagcc gcgccttgag ctgcaggaac tgctcgaaca
      481 gggtgccggc gccggcgcgc gccagcgttt cgacgatcag ctgcaggtcg ccacgcggct
      541 cgtacacggc gaggcggccc tggacttcca ccagttcgcc gtcgcgcggc atgaactcca
      601 tcagagcggc ggcgcggcga aacatcgcgc agcgcaattg gccctgcgca tccttcaagg
      661 aaaagtagca atgcccgctg gcagcgcgcg agaagccgga aacctcgccg cgcaccgcga
      721 cgggattgaa cctcgcctcc agcgcatcag caatcgcgcg gcatagcgcc ccgacctgcc
      781 agattcgcgg cgtcaatgct ggcgcggagt gctcggccat caggaaaatc gcggcttgcg
      841 ggccggttgc gcgctgaatt tagtccacag cgcctggaaa ccacgccgcc attggggaga
      901 ctcgccggaa aaccgcgatt ttgcgaagca agcctttgat ttcaccggat tttttattgc
      961 tcaaattgtg atcgatgtgt ccaactgaag gactggcgcg ggttgccgcg gcatgggggg
     1021 cgccttctac acaaagttat ccacagcttc tgtgagtgac gtcctacgaa ggcgcggcgt
     1081 tttcctacaa cgcgccgagt acacgataat cggccgctac tcagcattcg catcgcgcgg
//
LOCUS       DASRVO010000297         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_164277, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000297 DASRVO010000000
VERSION     DASRVO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164277"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1485)
                     /locus_tag="VFF05_03675"
     CDS             complement(118..1485)
                     /locus_tag="VFF05_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50934.1"
                     /translation="MRRRILALAVAACCAGVIPSAHADDAALKSEVEALRAEVAALKA
                     EVEQMRGVSAQAVASTVSTSSQAASVAVANAAPLTPNPGSAAPNPGSAAGEGTTLWGY
                     GQLDYNRPTARPADAQFDLSRAVIGFNHVFDESTRIYGELEWEHAIASAEDAGESEVE
                     QLYIEHALAPSYGVRAGLMLVPIGLLNEHHEPTQFYGVERNFVEQAIIPSTWREGGIA
                     GYGNTDSGWQWDIGLVTSPNLGKWDPTSDEGRAAPLGSIHQELQLAKAHDVSLWGRLN
                     WLGVPGLNIGAAFFAGKIGQDTSRQFDGDANFPADDSRLLLGEAHVRWQSGPWDFSAL
                     YTRGTISDTQSLNLSFLGQPTPVPKSFWGGYAQGAWRALEWNRSSLVPFLRYEEFNTG
                     AAYAAQPQGLGTPALPTQRVWTAGVNYYLNPNVVFKIDYQNFRYDDVLLNYGNRFDLG
                     IGYQF"
BASE COUNT          273 a          519 c          478 g          268 t
ORIGIN      
        1 cttgcgcgaa cgcggcctgc tgcgcctgct ccaccgtcat gtactgcacg gcataggcgg
       61 cctgcgcaac caccgtcgcg ccgacgccca gcggaagaag ccagcgcacc tgcatggtca
      121 gaattggtac ccgatgccca ggtcgaaacg gttgccgtaa ttcaacaaca catcatcgta
      181 gcggaaattc tggtagtcga tcttgaacac cacattcgga ttcaggtagt agttcacgcc
      241 cgccgtccac acgcgctgcg tcggcaatgc gggcgtaccc aacccttgcg gctgcgcggc
      301 atacgctgca ccggtgttga attcctcgta acgcaggaac ggcaccaggc tcgaccgatt
      361 ccattccagc gcacgccacg cgccctgcgc atagccgccc cagaacgatt tcggcaccgg
      421 cgtcggctgg ccgaggaaac tcaagttcag cgactgcgtg tcgctgatcg tgccgcgtgt
      481 atacaacgcg gaaaaatccc aggggccgga ttgccagcgc acgtgcgcct cgcccagcaa
      541 caggcgcgaa tcgtccgccg ggaaattcgc atcgccatcg aactgcctgc tcgtgtcctg
      601 gccgatcttg ccggcgaaga acgccgcacc aatgttgagc ccgggcacgc cgagccaatt
      661 cagcctgccc cacaacgaca catcgtgcgc cttggccagt tgcagttcct ggtggatgct
      721 gccgagcggc gcagcgcggc cctcgtcgga agtcggatcc catttgccca ggttggggct
      781 cgtgaccaag ccgatgtccc actgccagcc agaatccgtg ttgccgtagc cggcgatgcc
      841 gccttcgcgc caggtactgg gaatgatcgc ctgttcgacg aaattgcgtt ccacgccata
      901 gaactgggtc ggctcgtggt gctcgttgag caacccgatc ggcaccagca tcaggccggc
      961 gcgcacgccg tagctcggcg ccagcgcgtg ctcgatgtac agctgctcga cctcggattc
     1021 gccggcatct tccgccgagg cgatggcgtg ctcccattcc agctcgccgt agatgcgcgt
     1081 ggattcgtcg aagacgtgat tgaagccgat caccgcgcgg ctgagatcga actgcgcatc
     1141 ggctggacgc gccgtgggcc ggttgtagtc gagctggccg tagccccaca gcgtggtgcc
     1201 ctcgcccgcc gcactgccgg gattcggcgc agcggaaccg ggattcgggg tgaggggcgc
     1261 agcattcgcc acggcgacgg aagccgcctg tgacgaggtc gacacagtcg acgcgacggc
     1321 ttgcgcagat acgccacgca tctgctcgac ttcggccttg agcgcggcca cttcggcgcg
     1381 cagcgcctcg acttccgact tgagcgccgc atcgtcggca tgcgcggacg ggatcacgcc
     1441 ggcacagcag gcagctaccg ccagcgcgag tatacgacgt cgcattgttg caatctccat
     1501 tttgtcaatt catgtcgggt gcggtgtcgc gcgccggg
//
LOCUS       DASRVO010000298         1974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_61049, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000298 DASRVO010000000
VERSION     DASRVO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61049"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /gene="hemW"
                     /locus_tag="VFF05_03680"
     CDS             complement(<1..626)
                     /gene="hemW"
                     /locus_tag="VFF05_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZW50935.1"
                     /translation="MQGIAKTRRLPPLSLYIHIPWCVKKCPYCDFNSHNAPQALPQDE
                     YVDALLADLDQDLALARGRPLQSIFFGGGTPSLFSADAIARILDGAAQRLAFAPNIEI
                     TLETNPGTVEHGPFADYRRAGVNRISFGVQSFDDTALMRIGRIHDASQAERAVKSAQD
                     AGIENLNLDLMYALPQQTLAGALVDVEKAIMLQTPHLSHYQLTLEPNTA"
     gene            complement(705..1298)
                     /gene="rdgB"
                     /locus_tag="VFF05_03685"
     CDS             complement(705..1298)
                     /gene="rdgB"
                     /locus_tag="VFF05_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZW50936.1"
                     /translation="MKLVLASGNTGKLAELCEILADVDVELVAQSELRITDAEETGST
                     FVENAILKARHAARASGLPALGDDSGICVDALDGAPGLRSARYAGTHGDSAANIAKLL
                     DALRDVPDARRTAHFHCSIVLLRKPDDPAPLIAEGRWHGSILHAPRGAGGFGYDPIFF
                     DPQFGQSAAELDATTKNRISHRGLALAKLRELLSRFE"
     gene            complement(1295..1687)
                     /locus_tag="VFF05_03690"
     CDS             complement(1295..1687)
                     /locus_tag="VFF05_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZW50937.1"
                     /translation="MNAHVAMIAVLVRDYDEAIAWYRDALGFALLEDEDRGGDKRWVR
                     MAPSGNAHFSLLLARATTPAQSAAIGNQHGGRVGFFLHTDDFVRDHARLSAAGAQFEE
                     SPRHEAYGTVVVFRDLYGNRWDLIERRA"
     gene            complement(1684..>1974)
                     /gene="rph"
                     /locus_tag="VFF05_03695"
     CDS             complement(1684..>1974)
                     /gene="rph"
                     /locus_tag="VFF05_03695"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470803.1"
                     /note="RNase PH; tRNA nucleotidyltransferase; forms
                     hexamers in Bacillus subtilis; phosphoroltic 3'-5'
                     exoribonuclease; involved in maturation of tRNA precursors
                     and removes terminal nucleotides near CCA acceptor arms of
                     mature tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZW50938.1"
                     /translation="WLKARDKIKRDPLHGAVAAVSVGIYQGKPVLDLDYAEDSACDTD
                     MNVVMNDGGGFIELQGTAEGHAFRREELDALLGLAQKGIGELLQKQREALSA"
BASE COUNT          337 a          618 c          650 g          369 t
ORIGIN      
        1 gcggtgttcg gttccagcgt caactggtaa tgcgacaaat gcggtgtttg caacattatt
       61 gccttttcca catcgaccag cgcgccggcc aacgtttgct gcggcagtgc gtacatcaaa
      121 tccagattca ggttttcgat gccggcgtcc tgcgcggatt tcaccgcgcg ctcggcttgg
      181 ctcgcgtcgt ggatgcggcc gatgcgcatg agtgccgtgt cgtcgaaact ctgtacgccg
      241 aaactgatgc ggttcacgcc agcgcggcgg taatccgcga acggcccgtg ttcgaccgtg
      301 ccgggattgg tttccagcgt aatctcgatg tttggggcga aggcgagcct ttgcgccgcg
      361 ccatccagaa tccgtgcgat ggcgtccgct gaaaacaggc tgggcgtgcc gccaccgaaa
      421 aatatgcttt gcagcgggcg gccgcgcgcc agcgccaaat cctgatccag gtccgcgagc
      481 agcgcgtcca cgtattcatc ctgcggcaac gcctgtggcg cattgtgcga attgaaatcg
      541 caatacgggc acttcttcac gcaccacggg atgtggatgt acaaagacaa cggtggcaga
      601 cgccgcgttt tcgcgatgcc ttgaatggca tcgcgatcgg cggctgacga ggaggcagga
      661 cgccgactgg ggggcactcc agggatggac gttggcgagg ccattcactc gaaccgggac
      721 aacaactcgc gcagcttcgc caacgccagc ccacgatggc tgatgcggtt tttcgtggtg
      781 gcatcgagtt ccgcggcgct ttggccgaat tgggggtcga agaagatcgg gtcgtagccg
      841 aagccgccgg cgccgcgcgg tgcatgcaag atcgagccgt gccagcgacc ttcggcgatc
      901 agcggcgccg gatcgtcggg cttgcgcagc aggacgatgc tgcaatggaa atgcgcggtg
      961 cgccgcgcgt ccggcacgtc gcgtagcgcg tcgagcagct tggcgatgtt cgccgccgaa
     1021 tcgccgtgtg tgccggcgta gcgcgcggac cgcaggcctg gcgcaccgtc gagtgcgtcc
     1081 acgcagatgc cggaatcgtc gccgagcgcg ggcaggcctg atgcacgcgc cgcgtgccgc
     1141 gctttcagga tcgcattctc gacgaaggtg ctgccggttt cctcggcatc cgtgatgcgc
     1201 agttcggatt gcgcgacgag ttcgacatca acgtcggcga ggatttcgca caactcggcc
     1261 agcttgccgg tgttgccgct ggcgaggacg agcttcatgc gcgccgctcg atcaagtccc
     1321 agcggttgcc gtacaaatcc ctgaacacga cgacggtgcc gtaggcctca tggcgcggcg
     1381 attcctcgaa ctgcgcgcct gcggcgctca ggcgtgcgtg gtcgcggacg aagtcgtcgg
     1441 tgtgcaggaa aaagccgacg cggccaccgt gctggttgcc gatggcggcg ctttgcgcag
     1501 gcgtcgtcgc gcgcgccaac agcaggctga aatgcgcgtt tccggacggc gccatgcgca
     1561 cccagcgctt gtcgccgccg cgatcctcgt cttcgaggag cgcaaagccg agtgcgtcgc
     1621 gataccaggc gatcgcctcg tcgtagtcgc gcacgagcac ggcgatcatc gccacatgcg
     1681 cattcatgcc gacaacgctt cgcgctgctt ttgcagaagt tcgccaatcc ctttttgcgc
     1741 aaggccaagc agggcatcga gttcctcgcg ccggaaggcg tggccttcgg cggtgccttg
     1801 caactcgatg aagccgccgc cgtcgttcat caccacgttc atgtcggtgt cgcaggccga
     1861 atcctcggcg tagtcgagat caagcacggg cttgccctga taaatgccga ccgatactgc
     1921 cgcgaccgcg ccgtgcaaag gatcgcgctt gatcttgtcg cgcgctttca gcca
//
LOCUS       DASRVO010000299         1219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_191480, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000299 DASRVO010000000
VERSION     DASRVO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191480"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1219
                     /locus_tag="VFF05_03700"
     CDS             <1..>1219
                     /locus_tag="VFF05_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZW50939.1"
                     /translation="VEPATTDFAHKRLRNVVEEIAIASGVPVPEVYVIENEPAINAFA
                     AGYTPADAAVTVTQGCLDKLNREELQGVIAHEFSHVLNGDMRLNIRLMGVLFGILVIG
                     IIGRKIAANSGRSRDSGYAVLIGVAILAIGYIGVFFGRLIKAGISRQREYLADASAVQ
                     FTRQTRGISGALKKIGGLVDGSKLSSGETEEVAHMLFGDGVGYSALFATHPPLQLRIK
                     AIDPAFDPAELDAIAKAWANPVPVGDSDGAQVSISGFAPAAIASAMGVSGARTPQPVR
                     IVLPQADAEVSLTPRKVVTQVAHPGSDDYRTANAIHDTISEKLRALAYMGTRCQQVVF
                     ALAIDTDAAMRGTQLALLEKRFDANVRKGVEEVLAETDALHPMQRLPLAQLAFPALRR
                     QPRPQLQTFLIALN"
BASE COUNT          213 a          414 c          391 g          201 t
ORIGIN      
        1 cgtcgaaccg gcgacgaccg acttcgcgca caagcgcctg cgaaacgtgg tcgaggaaat
       61 cgccattgcc tccggcgttc ccgtcccgga ggtctacgtg atcgagaatg agcccgcgat
      121 caacgctttt gccgcgggct acacgccggc cgacgccgcc gtcaccgtca cgcagggctg
      181 cctggacaag ctcaaccgcg aggaactgca gggtgtgatc gcgcacgagt tcagccatgt
      241 gctcaacggc gacatgcgcc tgaacatccg tctgatgggc gtgctgtttg gcatcctcgt
      301 gatcggcatc atcggccgca agatcgccgc caacagcggg cgcagccgcg attcgggcta
      361 cgccgtgctg atcggcgtcg caattctcgc catcggttac atcggcgtgt tcttcgggcg
      421 cctgatcaag gctggcattt cgcgccagcg cgaatacctc gccgacgcct ccgcggtgca
      481 gttcacgcgg cagacgcgcg gcatctctgg cgcgcttaag aagatcggcg gattggtcga
      541 cggctcgaaa ctttccagcg gcgaaaccga ggaggtcgcg cacatgctgt tcggcgacgg
      601 cgtgggctac tcggcgcttt tcgccacgca cccgccgctg cagttgcgca tcaaggccat
      661 cgatccggcg ttcgatcccg ccgaactcga tgccatcgcc aaggcctggg cgaatccggt
      721 gccggtcggc gacagcgacg gcgcgcaggt gtcgatttcg ggattcgcgc cagcagcgat
      781 cgcctccgcc atgggggtga gcggagccag gacgccgcaa ccggtacgaa tcgtcttgcc
      841 gcaggcggac gccgaagtct ccttgacgcc caggaaagtg gtgacccagg tcgcccatcc
      901 ggggtccgac gactatcgaa ccgcaaacgc gatccacgac acgatttccg agaagctgcg
      961 cgccctggcc tacatgggta cgcgctgcca gcaggtggtg ttcgcgcttg ccatcgacac
     1021 cgatgcagcg atgcgcggca cgcagctcgc gttgctggaa aagcgcttcg acgcgaacgt
     1081 gcgcaagggc gtcgaggaag tcctcgccga aaccgatgcg ctgcacccga tgcagcgcct
     1141 gccgctggcg cagctcgcgt ttccggcgct gcgccgccag ccgcggccgc aattgcaaac
     1201 cttcctgatc gcgctcaac
//
LOCUS       DASRVO010000300         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_9172, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000300 DASRVO010000000
VERSION     DASRVO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9172"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1061)
                     /locus_tag="VFF05_03705"
     CDS             complement(<1..>1061)
                     /locus_tag="VFF05_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW50940.1"
                     /translation="WSVFRAVSGDPIPWKSAQLHVPQVDMWGPSGRSPDSTDNDPPNA
                     LDYNDAVALMRDHRAKLQSAMYAIGPSVVPADAAKHPIQTGGHAVFGEFFAMVDVPFK
                     YGSGWSATDDEHRAQVAVISAKLNEKVFDGANSVGKMLNIEGRDYRVVGVIGDWNPQP
                     RFYDVVNTGGFASESEDLYVPFNTAISAGMSNDGNTNCSVVPEAGFEGLQRSSCVWIA
                     YMAQLDDAAAVDAYRNYLEGFARTRYTGWAPNVRLRNLPDWLDHEHVVPSDTKVSLLI
                     ALGLLLVCLVNTAGLLLAKFLRRSSEIGVRRALGAPRLAIYAQFLTEGAIIGVVGGVI
                     GLVLTGIGVLSVGLVLPKA"
BASE COUNT          182 a          360 c          335 g          184 t
ORIGIN      
        1 ggcctttggc agcaccagac ccacgctgag cacgccgatg ccggtgagca ccaggccgat
       61 cacgccgccg accacgccga tgatcgcgcc ttcggtcagg aactgcgcgt agatcgccag
      121 gcgcggcgcg ccgagcgcgc ggcgtacgcc gatttcgctg ctgcggcgca ggaacttcgc
      181 aagcagcaac ccggccgtgt tgaccaagca cacgagcagc aggcccagcg caatcaacag
      241 cgataccttg gtgtcgctcg gcaccacgtg ctcgtgatcg agccagtccg gcagattgcg
      301 caggcgcacg ttcggcgccc agcctgtgta gcgcgtgcgt gcaaatcctt ccaggtagtt
      361 gcggtaggcg tcgaccgcag ccgcgtcgtc gagctgcgcc atgtacgcga tccacacgca
      421 gctcgaacgc tgcaagcctt cgaatcccgc ctcgggaacc acgctgcaat tcgtgttgcc
      481 gtcgttcgac atgccggcgg agatcgccgt gttgaacggc acgtacaggt cctcgctctc
      541 gctggcgaaa ccgccggtgt tgaccacgtc gtagaagcgc ggctgcggat tccagtcgcc
      601 gatcacgccg accacgcggt aatcgcggcc ttcgatgttg agcatcttgc cgacgctgtt
      661 ggcgccgtcg aacaccttct cgttgagctt ggcgctgatg accgcgacct gggcgcgatg
      721 ctcgtcgtcg gtcgcgctcc agccgctgcc gtatttgaac ggtacgtcga ccatcgcgaa
      781 gaattcgccg aagacggcat ggccgccggt ctggatcgga tgcttggccg cgtcggccgg
      841 caccaccgac ggtccgatcg catacatcgc cgattgcagt ttggcgcgat ggtcgcgcat
      901 caacgcaacc gcatcgttgt agtcgagcgc attcggtgga tcgttgtcgg tgctgtccgg
      961 agaacgcccg cttggacccc acatgtcgac ctgcggcacg tgcagctgcg cggatttcca
     1021 cgggatcgga tcgcccgaca ccgcacggaa caccgaccac g
//
LOCUS       DASRVO010000301         5276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_86980, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000301 DASRVO010000000
VERSION     DASRVO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86980"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..3967)
                     /locus_tag="VFF05_03710"
     CDS             complement(68..3967)
                     /locus_tag="VFF05_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HZW50941.1"
                     /translation="MWKRHVHRARLWLQALFAAVVILLALGVGLAQIALPWIASRPQR
                     IAAFLSDRLQRAVTLDGVTGVWERDGPLLALHGVHIAGTDAKSPGSTIPQAELKINFF
                     SALHRNQAWNEFRLVGLNLHLARDAQGQWQLSGIDAGNQAAADPKDSLLFSLGALVLR
                     DLHLTVQGPGNARPVVLLADEVRLVNSGSTHRVLARVRCADTPSSVDAVIDYDSDARD
                     GELYLGSTALDLAAIAHGYAWQGVQVERGAGRVQFWGWWQRDRLQRARIETDLTNVVL
                     TTSAPIALDAKRSIVPRTAFDRIAFGARWQRGDAGWSADVADLVLARQGAVATPARIH
                     LEKSHDAALPDPTTPDPTWMLRLDGANLSTPASVAMLSDALPPAWRRWLYVADPVGTL
                     HHADLRFADPRDFDVAADFDALAWHAVDALPGVTSLRGNLLGDAEGLNLHLPPHTAFG
                     VDEPHVFRQPLEFSQFAGDIAAWPGDGGWRIGTDALDFEGAGFGGQLRGAMQLHDDGS
                     RPALDLYAVINHGEVPAAKLFWPINIMPPDAVKWLDRALVSGGIANGRAVFHGDLADW
                     PFTNFAGRFEARAELNDTKLTYLPDWPSAEHVHAVADFVNNGLRVDADAAQALGNTVD
                     SASATIADLGEAVLDLDVSGSGSGKNLLDFLKATPIGTQHAAQLLGVSVGGSGKLKFN
                     LHLPVKQAEQRVLTGSVVISRADLVDAQYALRLQQANGTLKFSQDGFDTGDLPVTRDG
                     KPAMFRLAVGGFATDKDHAVEASLRGRFPVSDLLAYAPVLAPWADRIDGEADWTIGFN
                     ADRDDVKNPGQRVTVTSDLDGIDIALPAPLAKAANAALPLSLSVGLPVVGGSIDLQLG
                     GLMRLRGRLGSATQPFAARVDFSGDASAPLPASGFEIGGSAPLLDLSGWMDFAVAKSS
                     GGDDGLLSGVDLQAQDLRAWDRDFGAARFTLKPSKTELSLAFDGANIAGNMEIPLADF
                     RQRGVTAQFDRIYWPDVGETETSAMAGENPANVPPLHIRIGDFRLGDSRFGTTVVESY
                     PVANGTHFEQVSTHSKNVEMRARGDWTGKPGSDASDFSVDFSAHNLGRMLDTFGYAGV
                     VEDGATVAHIQGHWAGSPSMFALARLDGTLKVSVQEGRIPDADPGAGRIFGLFNLTAI
                     PRRLALDFGDFFKSGFSFDSIEGTFTLKDGNAFTSGLRVKGPAADILVNGRAGLKAHD
                     YDQTMEVTPHVGGTLMIGGAVIGGPVGAAAGAVLQGVFKKAINDVTRVRYSVTGSWEK
                     PVITEIAKETVKGEPAPQKQRPAKPAAPTPTAGGTGG"
     gene            complement(3977..>5276)
                     /locus_tag="VFF05_03715"
     CDS             complement(3977..>5276)
                     /locus_tag="VFF05_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZW50942.1"
                     /translation="LERAAFLHASDILRSVPPLAGDGQENAVAPPAQPIAELVRDGQE
                     IVVQVIKDPIGSKGARLTTNLSVPSRYLVLLPLAKAIGVSVRIEDDTERARLKEILQA
                     RALETGHGYIVRTNAEGQGADALAEDVAYLTKLWQSVQEAIVGARAGERVYEDLSLPL
                     RALRDLMRKEVEKVRVDSRETLERMLRFAGQFMPELAERIEHYPGERPILDLYGVEDE
                     IQRALKKEVPLKSGGYLVVDQTEAMSTIDVNTGAFLGHRNLEETVYRTNLEAAQSAAR
                     QLRLRNLGGIIIIDFIDMVDEEHKRQVLRTLEKSLAHDHAKTTVYEMTELGLVQMTRK
                     RTVESLERQLSEPCPACEGRGSIKTAETVTYEIFREITRAVRQFEAKKLLVLAAPKVV
                     ARILDEESAAVAELEEFIGKTIRFQPEEHYAQEQYDVVLL"
BASE COUNT          912 a         1741 c         1715 g          908 t
ORIGIN      
        1 tggcgttcgg cctgggcaag caaagtcatg gttattcgtg ttccagtcga ggtttgaatc
       61 gcaggtctta cccgccggtg ccaccggctg tcggtgtggg cgccgcgggt ttcgccgggc
      121 gctgcttttg cggcgccggc tcgcccttga ccgtttcctt ggcgatttcg gtaatcacgg
      181 gtttttccca actgccggtg acgctgtagc gcacgcgcgt gacatcgttg atcgccttct
      241 tgaacacgcc ctgcagcacc gcgccggccg ccgcgccgac cggcccgccg atcaccgcgc
      301 cgccgatcat cagcgtgccg ccgacgtgcg gggtgacttc catggtttgg tcgtagtcgt
      361 gcgctttcaa gccggcgcgg ccgttcacca agatatccgc ggccggaccc ttgacacgca
      421 atccggacgt gaacgcgttg ccatccttga gcgtgaaggt tccttcaatc gaatcgaaac
      481 tgaaaccgga cttgaagaaa tcgccgaaat ccagcgccag gcgacgcggt atcgcggtca
      541 ggttgaacag gccgaagatg cggccggcgc ccggatcggc atcggggatg cggccttcct
      601 gcaccgaaac cttgagtgtg ccgtccaggc gcgccagcgc gaacatggac ggcgacccgg
      661 cccagtggcc ctggatgtgc gcgaccgtcg caccatcctc gaccacgccc gcataaccga
      721 acgtgtcgag catgcgcccg agattgtgcg cggaaaaatc gacggagaaa tcggaagcgt
      781 cgctgccggg cttgccagtc cagtcgccgc gcgcgcgcat ttcgacgttt ttcgaatgcg
      841 tgctgacctg ttcgaaatgg gtgccgttgg cgaccggata gctttccacg acagtcgtgc
      901 cgaaacgcga atcgccgagg cgaaaatcac cgatgcggat gtgcagcggc ggtacgttgg
      961 ccggattttc gcccgccatc gcgctcgttt cggtctcgcc gacgtccggc cagtagatgc
     1021 ggtcgaactg ggcggtaacg ccgcgctggc ggaaatcggc caacggaatc tccatgttgc
     1081 ctgcgatgtt ggcgccatcg aaggccaggc tcaactcggt ttttgacggt ttcagggtga
     1141 atcgcgccgc tccgaaatcg cgatcccagg cgcgcaaatc ctgcgcctgc aagtcgacgc
     1201 cggagagcag gccatcgtcg ccgccactgg atttcgcgac cgcgaaatcc atccagccgg
     1261 aaagatcgag cagcggcgca ctaccgccga tctcgaaacc ggaagccggc agcggcgcgg
     1321 acgcatcgcc gctgaagtcg acgcgcgcag cgaacggctg cgtggccgat cccaggcgtc
     1381 cgcgcaggcg catgagtccg cccagttgca gatcgatgct gccgcccacg accggcaagc
     1441 caacgctgag cgacagcggc aacgcggcat tggcggcctt ggccagcggc gcaggcaagg
     1501 cgatgtcgat gccgtcgaga tccgacgtca cggtcacgcg ctggccggga ttcttcacgt
     1561 cgtcgcgatc ggcgttgaag cctatcgtcc aatccgcctc gccgtcgatg cggtcggccc
     1621 atggcgccag caccggcgca taggcgagta ggtcggatac gggaaaacga ccgcgcaggc
     1681 tggcttcgac ggcatggtcc ttgtcggtcg cgaacccgcc caccgcgagg cggaacatgg
     1741 ccggcttgcc gtcgcgcgtg acgggcagat cgccggtatc gaagccatcc tgcgaaaact
     1801 tgagcgtgcc gttggcctgc tgcaagcgca gcgcgtactg cgcatcgacg agatcggccc
     1861 tggatatgac cacgctgccc gtgaggacgc gttgctcggc ctgcttgacc ggcaggtgca
     1921 gattgaattt caacttgccg ctgccaccga cgctgacgcc gagcagttgc gcggcatgct
     1981 gagtgccgat cggcgtggcc ttgaggaagt cgagcagatt cttgccgctg cccgagcccg
     2041 acacatccag atcgagtacc gcctcgccga gatcggcgat cgttgcgctg gcgctgtcca
     2101 cggtgttgcc cagcgcctgc gccgcgtccg cgtcgacgcg caaaccgttg ttgacgaaat
     2161 cggcgacggc atgcacatgt tcggcggacg gccaatccgg caaataggtc agcttggtgt
     2221 cgttcaattc ggcacgcgct tcgaaacggc cggcgaaatt ggtgaacggc cagtcggcga
     2281 gatcgccatg aaacaccgcg cgcccattcg cgatgccgcc cgagacgagt gcgcgatcca
     2341 gccacttcac cgcatctgga ggcatgatgt tgatcggcca gaatagtttt gccgccggca
     2401 cttcgccgtg gttgatcaca gcgtacaaat ccagcgccgg acgcgaaccg tcgtcgtgca
     2461 gttgcatggc accgcgcaat tggccgccga atccggcgcc ttcgaaatcc agcgcatccg
     2521 tgccgatgcg ccagccgccg tcgccaggcc aggcggcgat atcgccggcg aattgcgaga
     2581 attcgagtgg ctgccggaac acatgcggct cgtccacgcc gaacgccgtg tgcggcggca
     2641 aatgcagatt gagaccttcg gcatcgccga gcaggttgcc gcgcaggctg gtcactcccg
     2701 gcagcgcatc gacggcgtgc caggccagtg cgtcgaaatc ggcggcgacg tcgaaatccc
     2761 gtgggtcggc gaaacgcagg tccgcatggt gtagcgtgcc caccggatcg gccacgtaca
     2821 gccagcgccg ccatgccggc ggcaacgcat cactgagcat ggccacgctc gccggcgtcg
     2881 atagattcgc gccatccagg cgcagcatcc atgtcggatc gggtgtggtc ggatcgggca
     2941 aggccgcatc gtgcgatttt tccagatgga tgcgcgccgg cgtggcgacg gctccctgcc
     3001 gggcgaggac gaggtcggcc acatccgcgc tccagcccgc atcgccgcgc tgccagcgcg
     3061 cgccaaatgc gatgcggtcg aaagcggtgc gcggcacgat gctgcgcttg gcgtcgagcg
     3121 cgatcggcgc cgacgtggtc aacaccacgt tcgtcaaatc cgtctcgatg cgggcgcgct
     3181 gcagccgatc gcgctgccac caaccccaga attgcacgcg cccggcgcca cgttcgacct
     3241 gcacgccctg ccaggcataa ccatgggcga tggcggcgag gtccagcgcc gtgctgccca
     3301 ggtacaactc gccgtcgcgg gcatcgctgt cgtaatcgat caccgcgtcc acggacgatg
     3361 gcgtgtcggc gcagcgcacg cgcgcgagga cgcggtgcgt gctgcccgaa ttgaccaggc
     3421 gcacctcgtc ggcgagcagc acgacaggcc tggcgttgcc ggggccctgc acggtcaggt
     3481 gcagatcgcg caacaccagc gcaccgagcg agaacagcag cgaatctttc ggatctgcgg
     3541 cggcctgatt gcccgcgtcg attccgctca attgccattg accttgcgca tcgcgagcca
     3601 gatgcaggtt caggccaacg aggcggaact cgttccaggc ctgattgcgg tgcagcgccg
     3661 agaagaaatt gatcttgagt tcggcctgcg gaatcgtgct gccgggcgac ttcgcatcgg
     3721 tcccggcgat gtgcacgccg tgcagggcca gcaacgggcc gtcgcgttcc cagacaccgg
     3781 tcacgccgtc gagcgtcacc gcgcgttgca agcggtcgct caggaaagcg gcgatgcgct
     3841 gcgggcgact ggcaatccac ggcagcgcga tctgcgccag gccgacgcct agcgcgagca
     3901 ggatcacgac agcggcgaac agcgcctgca gccaaagacg cgcgcggtgg acatgacgct
     3961 tccacatcac ccgcacctac agcagaacaa catcgtactg ctcctgcgcg taatgctcct
     4021 cgggctggaa gcgaatcgtc ttgccgatga attcctcgag ttcggcgacc gccgcggatt
     4081 cctcgtcgag gatgcgcgcg acgactttcg gtgccgcgag cacgagcaat tttttcgcct
     4141 cgaactggcg caccgcacgg gtgatctcgc ggaagatttc gtaagtgacg gtttcggcgg
     4201 tcttgatgct gccgcgaccc tcgcaggccg gacacggttc ggacaactgg cgttccaggc
     4261 tctccaccgt gcgcttgcgc gtcatctgca cgagaccgag ttcggtcatc tcgtacacgg
     4321 tggtcttggc atggtcgtgg gcgagggatt tttccagcgt gcgcagcacc tggcgcttgt
     4381 gctcctcatc gaccatgtcg atgaaatcga tgatgatgat gccgccgagg ttgcgcaggc
     4441 gcagctggcg cgccgccgac tgcgccgctt cgagattcgt ccggtacacg gtttcttcga
     4501 gattgcgatg accgagaaac gcgccggtgt tgacgtcgat ggtcgacatg gcctcggtct
     4561 ggtcgacgac gagatagccg cccgacttca acggcacttc cttcttcagc gcgcgctgga
     4621 tctcgtcctc gacgccatac aggtctaaga tcggccgctc gccgggataa tgctcgatgc
     4681 gttcggcgag ttccggcatg aactggccgg cgaagcgcag catgcgctcc agcgtttcgc
     4741 gcgaatccac gcgcactttt tcaacttcct tgcgcatcag gtcgcgcaga gcgcgcagcg
     4801 gcagcgacaa atcctcgtac acgcgctcac cggcgcgagc gcccacgatc gcttcctgca
     4861 ccgactgcca cagcttggtc agataggcca cgtcttcggc cagggcatcg gcgccctggc
     4921 cttccgcgtt cgtgcgcacg atgtagccgt ggccggtctc cagcgcgcgc gcttgcagga
     4981 tttccttcag gcgcgcccgc tcggtatcgt cctcgatgcg caccgacacg ccgatggcct
     5041 tggcaagcgg caacagcacc aggtagcgcg atggcaccga caggttcgtg gtcaagcgcg
     5101 cgcccttgct gccgatcggg tccttgatca cctgcacgac gatttcctga ccgtcgcgaa
     5161 ccagctcggc gatgggctgc gcgggcggcg cgacggcgtt ttcctggccg tcgccggcaa
     5221 gcggcggcac gctgcgcagg atgtccgagg catgcagaaa ggcggcacgc tccagg
//
LOCUS       DASRVO010000302         1410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_9189, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000302 DASRVO010000000
VERSION     DASRVO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9189"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /gene="aroA"
                     /locus_tag="VFF05_03720"
     CDS             <1..1317
                     /gene="aroA"
                     /locus_tag="VFF05_03720"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZW50943.1"
                     /translation="IDWTSTPAGPLRGELRIPGDKSISHRAIMLAALAQGTSRITGFL
                     EGEDTLATARAFAQMGVRIDAPRASERIVHGVDLRGLHASDGVIDCGNAGTGMRLLAG
                     LLAGQAFASTLTGDESLSKRPMRRVLEPLERMGARIESDNGLPPLRIAGKCDLRGIDY
                     ELPVASAQVKSAILLAGLYASGETIVREPHPTRDYTESMLRAFGWSVDFQPGNVRLSG
                     GHRLRATDIAVPADFSSAAFFIVAASIVPGSEIVLESVGMNPRRTGLLHALRAMGADI
                     RESNVRESGRRGQGGELVADLVVRHAPLHGIDVPAEIAPDMIDEFPILFVAAAAAHGT
                     TRIRGAAELRVKESDRIAVMAKGLGALGIRIEETPDGAIIEGGKFTGGEADSAGDHRC
                     AMSLAVAGQVARAPVRVRDCTNVATSFPGFMELANSHGFDLAAAPM"
BASE COUNT          239 a          447 c          479 g          245 t
ORIGIN      
        1 atcgattgga ccagcacacc ggcaggtccg ctgcgcggcg agttacgcat tcctggcgac
       61 aagtccattt cgcatcgggc gatcatgctc gccgcactcg cgcaaggcac atcgcgcatc
      121 acgggattcc tcgaaggcga agacacgctg gcaaccgcgc gcgcgttcgc gcagatgggc
      181 gtgcgcatcg acgcgccgcg cgcaagcgag cgcatcgtgc atggcgtcga tctacgcgga
      241 ttgcatgcgt cggatggcgt gatcgactgc ggcaacgccg gcaccggcat gcgcttgctg
      301 gcgggtctgc tcgcggggca ggcgttcgct tccacgttga ccggcgacga atcgctgtcg
      361 aaacggccga tgcggcgcgt gcttgagccg ctcgaacgca tgggcgctcg catcgaatcc
      421 gataacggct tgccgccgtt gcgtatcgcc gggaaatgcg atttgcgtgg catcgactac
      481 gaactgccgg ttgccagcgc gcaggtgaaa tccgcgatcc tgctcgctgg actttacgcg
      541 agcggcgaaa cgatcgtgcg cgagccgcat ccgacgcggg actacacgga aagcatgctg
      601 cgcgcattcg gctggtcggt cgatttccag ccaggcaacg tgcggctttc cggcggccat
      661 cgcctgcgcg caacggatat tgccgtgccg gcggattttt cctcggccgc attcttcatc
      721 gtcgccgcga gcatcgtacc cggctcggaa atcgtgctgg aaagcgtcgg catgaatccg
      781 cgccgcaccg gattgctgca tgcgttgcgc gcgatgggcg ccgatattcg cgaatcgaat
      841 gtgcgtgaat cgggaaggcg cgggcagggc ggcgagttgg tcgccgatct cgtcgtgcgg
      901 catgcgccgt tgcacggcat cgacgtgcca gcggagatcg cgccggacat gatcgacgaa
      961 tttccgatcc tgttcgtggc cgcggcggcg gcgcacggca ccacgcgcat ccgcggcgct
     1021 gccgaactgc gggtcaagga atccgaccgc atcgcggtga tggccaaggg cctgggcgca
     1081 ctcggcattc gcatcgagga aacgccggac ggcgcgatca tcgaaggcgg aaagttcacg
     1141 ggcggcgagg ccgattcggc cggcgatcac cgttgcgcca tgagtttggc cgtggccggg
     1201 caggtcgcgc gcgcacccgt gcgcgtacgc gattgcacca acgtggccac ctcgttcccg
     1261 gggttcatgg aactggccaa ttcgcatggt ttcgatctcg cggcagcacc catgtaaagt
     1321 cgtgcggggc tttccggtaa aatggccgga tgcccgacaa aatcgtaccg gtactcacca
     1381 tcgacggccc ttccggttcc ggcaagggca
//
LOCUS       DASRVO010000303         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_217187, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000303 DASRVO010000000
VERSION     DASRVO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217187"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="lptF"
                     /locus_tag="VFF05_03725"
     CDS             <1..942
                     /gene="lptF"
                     /locus_tag="VFF05_03725"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HZW50944.1"
                     /translation="LTLLVPLAAFLGVQMAYGRLYRESEMAVFAASGLPASGLLRPLA
                     LFGVPLAIAIALVSFWLAPAAVRQAQALQEEASRSLIIAGLEPGRFVELPKNDGVMYV
                     EEMNADGTKFQQLFLESERTNAKDGSIGINIVTAASGELFHDADGAQRFLGLNDGFRV
                     EGTLGADNWRLMHYMRNDIQLPDNDTDTTTDSVKRSAPTSRLLASVDPVQRVELQWRL
                     AAPISVLVLVLLGLPLAKSSPREPRYARLLIALLAWFVYYNCLAIARSRLTQGALDPH
                     LGFWLVQIPTAMLAVYLIWRSDKLPRPRLARARAGET"
     gene            942..2042
                     /gene="lptG"
                     /locus_tag="VFF05_03730"
     CDS             942..2042
                     /gene="lptG"
                     /locus_tag="VFF05_03730"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HZW50945.1"
                     /translation="MRMVAFKQVDKLVAISVLGAVLVAWGFLVGFDAFRAFVGEVNDI
                     GTGQYTLSKAVAYTLPTVPRRGYQFFGYAALIGGLLGMGTLATSGELTALRAAGLSKL
                     RICVSVIVALTALTVVVMLMGETVAPAGERKAEALELAAKSRDVAIGRGGSLWARDGR
                     NVVNAKHGRTRAGKHGPAVELQDVRVFEFDDAGKLTVLSLAKTATHADGEWTFHDVRR
                     TQFGKASATSTTQAQAQWKSMLDPNVLAVSMIHPEYLSIGDLSRNIDYMQHNRQDAGS
                     YQRAYWGRIFYPLNVLVLAFCAVPFAFGTLRTGGLGKRLFIGIALAISYYFFQSAVVS
                     MGAVYNFNLALANAMPSLLLAGAATVYFRRYG"
     gene            complement(2039..>2422)
                     /locus_tag="VFF05_03735"
     CDS             complement(2039..>2422)
                     /locus_tag="VFF05_03735"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZW50946.1"
                     /translation="AYDLLPLIGLWFVAAVLALAITGGALDTHTPAGKVLVQGFALVL
                     SAAYFVVSWSRGGQTIGMRAWRLRVVAADGNRVSWSHAFVRFFIALVSLAALGAGFWW
                     ALVDAQRRTWHDMAACTHLVRLSKT"
BASE COUNT          414 a          793 c          792 g          423 t
ORIGIN      
        1 ctcaccttgc tggtgccgct ggcggcgttc ctcggcgtac agatggcgta tgggcgcttg
       61 taccgcgaga gcgaaatggc ggtgttcgcc gcctccggtc tgcccgcttc cggcttgttg
      121 cggccgctcg cgttgttcgg cgtgccgctc gcgatagcca ttgccctcgt ctcgttctgg
      181 ctcgcgccag ccgcagtgcg ccaggcgcag gcgctgcagg aagaagccag ccgctcgctc
      241 atcatcgccg gactcgaacc cggccgcttc gtcgagctgc ccaagaacga cggcgtcatg
      301 tacgtcgagg aaatgaacgc cgacggcacc aagttccagc aactgttcct ggaaagcgaa
      361 cgcacgaatg cgaaggacgg cagcatcggc atcaatatcg tcaccgcggc cagcggcgaa
      421 ctctttcacg acgccgacgg cgcacaacgc ttcctcggtc tcaacgacgg cttccgcgtc
      481 gagggcacgc ttggcgcgga caattggcgc ctgatgcatt acatgcgcaa cgacatccag
      541 ttgccggaca acgacaccga caccacgacc gattcggtca agcgcagcgc gccgacctcg
      601 cgattgctgg cgagcgtcga tccggtgcag cgcgtggaac tgcaatggcg cctggccgcg
      661 ccgatttcgg tgctggtgct ggtgctgctc ggcctgccgc tggccaaatc cagcccgcgc
      721 gaaccgcgct atgcgcgcct gttgatcgcg ctgttggcct ggttcgtgta ctacaactgc
      781 cttgcgattg cgcgttcccg gctcacgcaa ggtgcacttg atccgcatct gggattctgg
      841 ctcgtacaaa tccctacagc gatgcttgcc gtttatttga tctggcgcag cgacaagttg
      901 cctaggccgc ggctcgcgcg tgcgcgggcg ggagaaactt gatgcgcatg gtcgcgttca
      961 agcaagtcga caaactggta gcgatttccg tgcttggcgc ggtgctggtg gcgtggggat
     1021 tcctggtcgg cttcgacgcg ttccgcgcct ttgtcggcga ggtcaacgac atcggcaccg
     1081 gccagtacac gctgagcaag gcggtggcct acaccctgcc caccgtgccg cgccgcggct
     1141 accagttctt cggttacgcc gcgctgatcg gcggcctgct cggcatgggc acgctcgcga
     1201 ccagcggcga gttgaccgcg ttgcgcgcgg cggggctgtc gaaactgcgc atctgcgttt
     1261 cggtgatcgt ggcgctgacc gcgctgaccg tggtggtgat gctgatgggc gaaaccgtcg
     1321 ctccggccgg cgagcgcaag gccgaggcgc tggaactcgc agccaaatcc agggacgtcg
     1381 ccatcggccg cggtggcagc ctgtgggcac gcgatggacg caacgtggtc aatgccaagc
     1441 acggtcgcac gcgcgccggc aaacacggtc cggcggtgga attgcaggac gtacgcgtgt
     1501 tcgagttcga cgatgccgga aagttgaccg tgctgtcgct ggcgaaaacc gccacccatg
     1561 ccgacggcga atggaccttt cacgacgtgc gccgcaccca gttcggcaag gcctcggcga
     1621 cgagcacgac gcaagcccag gcgcagtgga aatcgatgct cgatccgaac gtgctcgcag
     1681 tgtcgatgat ccatccggaa tacctgtcga tcggcgacct gtcgcgcaac atcgattaca
     1741 tgcagcacaa ccgccaggat gccggcagct accagcgcgc ctactggggc cgcatcttct
     1801 acccgctcaa cgtgctggtg ctggcgttct gcgccgtgcc gttcgcgttc ggcacgctgc
     1861 gcacgggcgg cctcggcaag cgcctgttca tcggcatcgc gctggcgatc tcgtactact
     1921 ttttccagag cgccgtggtc agcatgggcg cggtatacaa tttcaacctg gccctggcga
     1981 acgcgatgcc atcgctgttg ctggccggcg cggcgacggt ttactttcgc aggtacggct
     2041 aggttttcga caggcgcacc aagtgcgtgc acgccgccat gtcatgccag gtgcgccgct
     2101 gcgcatcgac cagtgcccac cagaatcccg cgccgagcgc ggcgagcgaa accagcgcga
     2161 tgaagaaacg cacgaaggcg tgtgaccacg acacgcgatt tccatccgca gcgaccacgc
     2221 gcagccgcca cgcgcgcatg ccgatggttt ggccaccacg cgaccacgag acgacgaaat
     2281 acgcggcgct gagtacgagt gcgaatccct gcacgaggac tttgccggct ggcgtgtgcg
     2341 tgtcgagcgc gccgccggtg atggcgaggg cgagcacagc ggcgacgaac cacagcccga
     2401 tcaggggcag caggtcgtag gc
//
LOCUS       DASRVO010000304         1334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_138588, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000304 DASRVO010000000
VERSION     DASRVO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138588"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..823)
                     /gene="radC"
                     /locus_tag="VFF05_03740"
     CDS             complement(149..823)
                     /gene="radC"
                     /locus_tag="VFF05_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZW50947.1"
                     /translation="MTIRDWPESERPREKLLERGAAALSDAELLAVFIGSGRRGQTAV
                     DVGRDLLARHGSLKSLLEREVTALSAEPGLGTAKSCRLHAALELGRRYLACDLRQPEA
                     LANPAACADYLRARIGAYPYEVFACVFLDNRHRILAFEELFRGTIDGASVHPREVVRR
                     CLAHNAAAVILAHNHPSGVAEPSQADRAITAELKRALETIQVRVLDHFIIGAGLPMSF
                     AARGLL"
     gene            919..>1334
                     /locus_tag="VFF05_03745"
     CDS             919..>1334
                     /locus_tag="VFF05_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HZW50948.1"
                     /translation="MFQQPLANLRILLGVSGGIAAYKACELVRRLRDAGADAQVVLTA
                     NAARFVAPLTFQALSGHPVRSGLWDEAAEAAMGHIELARWAQRIVIAPASADVIARLA
                     HGIADDLLTTLCLASAAPLAVAPAMNLRMWTHPATQA"
BASE COUNT          221 a          442 c          463 g          208 t
ORIGIN      
        1 gagcaactgc ggattgcgct cgcgcacgag cgcgcgcgcc gtcgccgcat ccggcacgcg
       61 acgttcgacc tcgtcagcgg gaaactccgt gggataagca aaaatcacgc aaaaccatcc
      121 tccccaccgg agtatagccc gaaccgcgtc aaagcaagcc gcgtgcggcg aacgacatcg
      181 gcagcccggc gccgatgatg aagtgatcga gcacgcgcac ctggatggtt tccagcgcgc
      241 gcttgagttc ggcggtgatc gcgcggtcgg cctgactcgg ctcagccacg ccggacgggt
      301 ggttatgggc gaggataacc gccgcggcat tgtgcgcgag gcagcgccgc accacttcgc
      361 gcgggtgcac gctggcgccg tcgatggtgc cgcggaacag ttcctcgaac gccaggatgc
      421 gatgccgatt atcgaggaat acgcaagcaa agacctcgta aggataggcg ccgatgcgcg
      481 cgcgcaggta gtcagcgcag gcggccggat tcgccaaggc ttccggttgg cgcagatcgc
      541 aggcgaggta acggcgaccc agttccaggg cggcatgcag gcggcaggat ttggccgtac
      601 ccaggcccgg ttcggcggag agtgccgtca cctcgcgttc cagcaaggat ttcaggctgc
      661 cgtgacgcgc cagcaggtcg cgaccgacat ccacggcggt ctggccgcgc cggccgctgc
      721 cgatgaacac ggcgagcaat tcggcatcgg acaaggccgc cgcgccgcgt tcaagcagct
      781 tttcgcgggg gcgttcggat tcgggccagt cgcgtatcgt catggtcgca gtgtgagcgc
      841 ggcgcggatt gtgcgctgcc ggcgggcacg cgaattccgg taagctaatg acctttcgtc
      901 gtgtcggaat tttcccgcat gtttcagcag ccgctggcca acctgcgcat cctgctcggc
      961 gtgtccggcg ggattgccgc gtacaaggcc tgcgagttgg tgcgccgtct gcgcgatgcc
     1021 ggcgcggacg cgcaggttgt gctcaccgcc aacgccgcgc gcttcgtcgc gccgctgacg
     1081 ttccaggcgc tttcggggca tccggtgcgc agcggactct gggatgaggc tgccgaagcg
     1141 gcgatgggac acatcgaact ggcgcgctgg gcgcaacgca ttgtcatcgc tccagccagc
     1201 gccgacgtca tcgcgcgcct ggcgcacggc atcgccgacg atctgctgac cacgctgtgc
     1261 ctggccagcg ccgcgccgct cgccgtcgcg ccggcgatga acctgcgcat gtggacacac
     1321 ccggccacgc aggc
//
LOCUS       DASRVO010000305         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_61214, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000305 DASRVO010000000
VERSION     DASRVO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61214"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VFF05_03750"
     CDS             <1..665
                     /locus_tag="VFF05_03750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZW50949.1"
                     /translation="AVTGGTEACVTETTMKAWEAMRVIADDTCRPFCKQRRGMVLGEG
                     AGIFVLEEWEHAKARGASILAEFAGSGATADAIDMVFPAEATVARAIELALIDAGLNP
                     ADVDYINAHGTGTPANDPTESKAIRRVFGAQADKIAVSSTKSMHGHALGAAGAIELVA
                     AIGSLRDGVIAPTANFIDPDPECDLDYVPNTAREKPVRAALSNSFAFGGLNAVVALKR
                     AD"
     gene            717..1592
                     /locus_tag="VFF05_03755"
     CDS             717..1592
                     /locus_tag="VFF05_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZW50950.1"
                     /translation="MASFRFLQLDVFPASPGGGNPLGVVFDAPELDQAQMQRVAHWLN
                     LVETTFVLPPENADADYRLRIFTPNAEIPFAGHPTIGSAHAVLDCGFAAARDGQLIQQ
                     CGAGLLPIRVEGTDATRELFVRAPAARIVREGSDDASLRAALGFQELGKLVPALVDGG
                     RRWWIAELAGEDAVRAWQPNHAAIKALARADACMGLCVFARCANAEYDVVVRAFPAGA
                     GIVEDPASGAANGLIAAYIAQCEPHGELARGYRVSQGREMDHDAALAVRIDADISVWV
                     GGRTRTVIEGSLCWE"
     gene            1695..2777
                     /locus_tag="VFF05_03760"
     CDS             1695..2777
                     /locus_tag="VFF05_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW50951.1"
                     /translation="MTRVIIVDDSKLIRNAGAKMLGSEFDVVVAEDGAAAWTLLLQDR
                     TIEVVFTDLDMPNLNGYELLRDIRTATDLRIRGLPVIVVTGVEDDEVARVRALELGAT
                     DFITKPFATIDLLARARAHASHQRETAQLRVQATLDPLTGLANKTGFLDRLQQDMAYA
                     RRHRQPLMLARLEIGNFRAIFLQRGKAVGERLLAHVAALLRAAIRTEDTAGRVTLGGF
                     ALALPGGEQQGIERMLERLRTGIANDWQALVGDGMPMELAGAVWQPPLESPGDAAAVL
                     DQGQLRPLAAAETTATPAPTVTPIVAAPEPAPLRIDPLLEQIDRGDVQPALAEMPRVL
                     PRLMPLLRLLTPAQRTALIGFLQPDA"
     gene            complement(2799..>3369)
                     /locus_tag="VFF05_03765"
     CDS             complement(2799..>3369)
                     /locus_tag="VFF05_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZW50952.1"
                     /translation="TGFRLGLAGAQGRLGIKPDLSVFAKGIGGGFPVAAMGGRADIMA
                     LVANGTVSMAGTYSANGIAIAAANAALDELNTPNLHRNLDAVSDELRAGLEKVLEDAG
                     LPAYVVGMGPLMQVWFAREPIHNYRDAERHADQKIFDVWWRGMLARGVLFHPGAYENL
                     FVSTAHTREDIAATLVEAKQVAQELARQA"
BASE COUNT          569 a         1156 c         1108 g          536 t
ORIGIN      
        1 ttgccgtgac cggcggcacc gaggcctgcg tgaccgagac aacgatgaag gcctgggagg
       61 cgatgcgcgt catcgccgac gacacctgcc ggccgttctg caagcagcgc cgcggcatgg
      121 tgctgggcga aggcgcgggc attttcgtgc tcgaggagtg ggagcatgcg aaagcgcgcg
      181 gcgcgagcat cctcgccgaa ttcgccggca gcggcgcaac cgccgacgcc atcgacatgg
      241 tgtttccggc ggaggccacg gtcgcgcgtg cgatcgaact cgcgcttatc gacgccggac
      301 tcaatcctgc cgacgtcgat tacatcaacg cccacggtac cggcacgccg gccaacgatc
      361 cgaccgaatc caaggcgata cgccgcgtgt tcggcgcgca ggccgacaag atcgcggtgt
      421 cctcgaccaa atccatgcac ggccacgcgc tcggcgccgc cggcgcgatc gaactcgtcg
      481 ccgcgatcgg aagcttgcgc gatggcgtga ttgcgccgac cgcgaatttc atcgatcccg
      541 atcccgaatg cgatctcgac tacgtgccga acacggcgcg cgaaaaaccc gtgcgtgcgg
      601 cgctcagcaa ctcatttgcg ttcggcgggt tgaacgcggt ggtggcgctg aaacgcgccg
      661 actgatttca acgccgtgca tcggctatca tcgcgccccc aatcggtgcg gtgcgcatgg
      721 cctcatttcg attcctccag ctcgacgtct ttccggccag ccccggcggc ggcaatccgc
      781 tcggcgtcgt gttcgatgcg ccggaactcg atcaagcgca gatgcagcgt gtggcgcact
      841 ggctcaatct cgtcgaaacc acgttcgtgc tgccgccgga aaacgcggat gcggattacc
      901 ggctgcgcat cttcacgccg aatgccgaga ttccatttgc cggacatccg accatcggca
      961 gcgcacacgc ggtgctcgac tgcggattcg cggcagcgcg cgacggccaa ctgatccagc
     1021 aatgcggcgc cggattgttg ccgattcgcg tcgaaggcac ggacgcgaca cgcgaactgt
     1081 tcgtgcgcgc acccgccgcg cgcatcgtgc gcgaaggcag cgatgacgcc tcgctgcgcg
     1141 ctgcgctcgg attccaggag cttggaaaac tcgtacccgc cttggtcgac ggcggccggc
     1201 gctggtggat cgccgaactc gccggcgaag acgcggtgcg cgcgtggcag ccgaaccacg
     1261 ccgcgatcaa ggcgctggcg cgcgcggatg cctgcatggg actgtgcgtg ttcgcgcgct
     1321 gcgcgaatgc cgagtacgac gtcgtcgtgc gcgcgttccc ggccggcgcg ggcatcgtcg
     1381 aggatccggc ttccggcgcc gcaaacggac tcatcgccgc ctacatcgcg caatgcgaac
     1441 cgcatggcga actcgcgcgc ggctaccgcg tcagccaggg ccgcgaaatg gaccacgatg
     1501 cggcactcgc cgtgcgcatc gatgccgaca tttcggtctg ggtcggcggg cgcacgcgca
     1561 cggtgatcga gggcagcttg tgctgggaat gaccggcacc ggccatcctt gccctttccg
     1621 ccggccggca tgacaaggta cagtcgacgg gaaatttcga tgcatctcca cctgccaggc
     1681 gaccgccgca cgtcatgact cgcgtcatca tcgtcgacga ctccaaactg attcgcaatg
     1741 ccggcgccaa gatgctcggc agcgagttcg acgtggtcgt cgccgaggac ggcgccgcgg
     1801 cgtggaccct gctgctgcag gaccgcacca tcgaggtcgt gttcaccgac ctggacatgc
     1861 cgaatctgaa cggctacgag ctgctgcgcg acatccgcac ggcgaccgat ttgcgcatcc
     1921 gcggtttgcc ggtgatcgtc gtcaccggtg tcgaagacga tgaggtggcg cgcgtgcgcg
     1981 cactggaact gggcgcgacg gatttcatca ccaaaccgtt cgcgaccatc gacctgctcg
     2041 cgcgtgcgcg cgcccacgca agccatcagc gcgagaccgc gcaattgcgc gtgcaggcca
     2101 cgctggatcc gcttaccgga ctggcgaaca aaaccggctt cctcgatcgc ctgcagcagg
     2161 acatggccta cgcgcgccgc caccgccagc cgctgatgct ggcgcgcctc gagatcggga
     2221 atttccgcgc gattttcctg cagcgcggca aggcggtcgg cgaacgcctg ctcgctcacg
     2281 ttgcagcgct gctgcgtgca gcgatccgca ccgaagacac ggccgggcgc gtgactctgg
     2341 gcgggttcgc cctggcgctg cccggcggcg aacaacaagg catcgagcgc atgctcgaac
     2401 gcttgcggac gggaatcgca aacgactggc aagcgctggt cggcgatggc atgccgatgg
     2461 agcttgccgg cgcggtgtgg cagccgccgc tggaatcccc gggcgacgcg gccgcggtac
     2521 ttgaccaggg ccaactgcgg ccgttggccg ctgcggaaac cacggcaacg ccagctccga
     2581 ctgtcacacc gattgtcgcc gccccagagc cggcgccact acgcatcgat ccgctgctgg
     2641 agcagatcga tcgcggagac gtgcaacccg cgctcgcgga aatgccgcgg gtgctgccgc
     2701 gcctgatgcc gctgctgcgc ctcctcacgc cggcgcagcg tacagcgctg atcggtttcc
     2761 tgcagccgga cgcctgatcc aggcgcgatg gcgcagaatc aggcctggcg cgcgagctcc
     2821 tgcgccacct gtttcgcttc caccagtgtg gccgcgatgt cctcgcgcgt gtgcgcggtg
     2881 gagacgaaca ggttttcgta agcgccggga tggaacagca cgccgcgcgc gagcatgcct
     2941 cgccaccaga catcgaaaat cttctggtcg gcgtggcgtt cggcgtcgcg gtagttgtgg
     3001 ataggctcgc gcgcaaacca cacctgcatc agcggtccca tgcccacgac ataggccggt
     3061 aatccggcgt cctcgaggac tttttccaaa cctgcgcgca actcgtcaga gaccgcatcc
     3121 agattcctgt gcaggttcgg cgtgttcaac tcgtccagcg ccgcgttcgc ggctgcgatg
     3181 gcgatgccat tcgcgctgta ggttccggcc atcgacacgg tgccgttcgc gaccagcgcc
     3241 atgatgtcgg cgcgcccgcc catcgccgcg accggaaagc cgccgccgat gcctttcgcg
     3301 aaaacagaca ggtcgggctt gatcccgagg cgcccctgcg cgccggcgag accgagacga
     3361 aaaccggtg
//
LOCUS       DASRVO010000306         1228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_372546, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000306 DASRVO010000000
VERSION     DASRVO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372546"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFF05_03770"
     CDS             <1..265
                     /locus_tag="VFF05_03770"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZW50953.1"
                     /translation="NAVNFPNTRMARAGKARLCIANRNRPNMIGQLSHLLGEAGVNIA
                     QMHNASRGDIAYTLIDLDSAASDALVAAIRRIEGILSVRAISA"
     gene            278..>1228
                     /gene="pheA"
                     /locus_tag="VFF05_03775"
     CDS             278..>1228
                     /gene="pheA"
                     /locus_tag="VFF05_03775"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZW50954.1"
                     /translation="MPKKKSAAAAPSLADARSRIDAIDRSIQQLIAERANWAHQVGKA
                     KGKLAAAVDYYRPEREAQVLRMVVDRNEGPLSDDVLVHVFREIMSACLAQQEPLKIGY
                     LGPEGTFSQQAVTKHFGRSAHGLPLATIEEVFQEVESGNADFGIVPVENSGQGTIQIT
                     LDMFLTSNLKICGEVELRVRQFLLSRSGRIEDIERVYSHPQSFAQCKAWLRGNLPKAE
                     KIAVSSNAEAARRARNADDAAAVAGESAGHVYGLKKVIMTPIEDRADNTTRFLVVGRQ
                     IFPPSGHDRTSILVFIKDKPGALFHVLSPFARNGISMNRIE"
BASE COUNT          232 a          403 c          391 g          202 t
ORIGIN      
        1 caacgccgtg aacttcccga acacgcgcat ggcgcgcgcg ggcaaggcgc gcctgtgcat
       61 cgcgaaccgc aaccggccga acatgatcgg ccagctttcg cacctgctcg gcgaagccgg
      121 cgtcaacatc gcgcagatgc acaacgcttc gcgtggcgac atcgcctaca cgttgatcga
      181 tctggattcg gcggccagcg atgcgctggt cgctgccatc cgcaggatcg aggggattct
      241 ctcggtgcgt gccatttccg cctgagcgag catcatcatg ccgaagaaaa agtccgccgc
      301 cgccgcacct tcgctcgccg atgcgcgttc gcgcatcgac gcgatcgacc gcagcatcca
      361 gcaattgatc gccgaacgcg cgaactgggc gcatcaggtc ggcaaggcga aaggcaagct
      421 cgccgcggcg gtggactact accggcccga gcgtgaggcg caggtgctgc gcatggtcgt
      481 ggaccgcaac gaaggcccgc tgtccgacga tgtgctcgtg cacgtgtttc gcgaaatcat
      541 gtcggcgtgc ctggcccagc aagagccgct aaagatcggc tatctcggcc cggaaggcac
      601 cttcagccag caggcggtga ccaagcattt cggccgctcc gcgcacggcc tgccgctggc
      661 gaccatcgag gaagtgttcc aggaagtcga gagcggcaac gccgatttcg gcatcgtgcc
      721 ggtggaaaat tccgggcagg gcacgatcca gatcacgctc gacatgttcc tgacctcgaa
      781 cctgaaaatc tgcggcgagg tcgagttgcg cgtacgccag ttcctgcttt cgcgctcggg
      841 ccgcatcgag gacatcgagc gcgtgtattc acatccgcag tcgttcgcgc agtgcaaggc
      901 ctggttgcgc ggcaatctgc cgaaggccga gaagattgcg gtgtcgagca acgccgaggc
      961 cgcgcgccgt gcgcgcaatg ccgacgacgc ggcggcggtt gcgggcgaat cggcggggca
     1021 tgtgtacggc ctgaaaaagg tcatcatgac gccgatcgag gatcgcgccg acaacaccac
     1081 gcgctttctc gtcgtcggcc ggcagatctt tccgccgtcc ggccacgatc gcacctcgat
     1141 cctggtgttc atcaaggaca agcccggcgc gctgttccac gtactcagtc cgttcgcacg
     1201 caacggcatc agcatgaacc gcatcgag
//
LOCUS       DASRVO010000307         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_269390, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000307 DASRVO010000000
VERSION     DASRVO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269390"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..876
                     /gene="ubiE"
                     /locus_tag="VFF05_03780"
     CDS             127..876
                     /gene="ubiE"
                     /locus_tag="VFF05_03780"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HZW50955.1"
                     /translation="MNEAPTTHFGYRDVPVAEKQKLVGRVFTSVAAKYDLMNDLMSFG
                     VHRLWKRWFAATSGVRAGDAVLDLAGGTGDIAALLLPLVGERGNVVIGDINAAMLHTG
                     RDRLLDHGHVRNLEYAQLNAEALPFPDASFDAVTIAFGLRNVTDKDAALCEMYRVLKP
                     GGRALILEFSAVQSEFLKPLYDFHSFKVLPRLGAMIAGDAESYRYLAESIRKHPDQAT
                     LKAMMDAAGFARVDVRNLSAGIVAIHRGYRI"
     gene            complement(893..>1149)
                     /locus_tag="VFF05_03785"
     CDS             complement(893..>1149)
                     /locus_tag="VFF05_03785"
                     /inference="COORDINATES: protein motif:HMM:NF022608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HZW50956.1"
                     /translation="CARRFVSRCRLAAMTQKFKIGDHVAWNSEAGYVSGTIVKVHTRD
                     FDYKGYTHHATPDDPQYEIKSDKTDHVAAHKGAALRKIRG"
BASE COUNT          191 a          371 c          351 g          236 t
ORIGIN      
        1 acggccacga caccggcatc ttttcctggg acgtgctgca cgatctgggc gtcaaccacg
       61 acgccaattg gcaggcgtat ctgtcggcac tcgaagccgc gggactgagt cgttagactt
      121 ggcgccatga atgaggcgcc caccacccat ttcggctatc gcgacgtgcc cgtcgcggag
      181 aagcaaaagc tcgtcggccg cgtgttcacg tcggttgcgg ccaagtacga cctgatgaac
      241 gacctgatgt cgttcggcgt gcaccggctg tggaagcgct ggttcgcggc gacctcgggc
      301 gtgcgcgcgg gcgatgccgt attggatctg gccggcggca ccggcgacat cgccgcgttg
      361 ctgctgccgc tggtcggcga acgcggcaac gtcgtcatcg gcgacatcaa tgccgcgatg
      421 ctgcacaccg gccgcgaccg cctgctcgat cacggccatg tgcgcaacct tgagtatgca
      481 caactcaacg ccgaggcctt gccatttccc gatgcgagtt tcgatgcggt gacgatcgcc
      541 ttcggcctgc gcaacgtcac cgacaaggac gccgcgttgt gcgagatgta ccgcgtgctc
      601 aaacccggcg gccgcgcgct cattctggaa ttctcggccg tgcaatccga attcctcaag
      661 cccctgtacg acttccactc gttcaaggtg ctgccgcgcc tgggtgcgat gatcgccggc
      721 gatgcggaaa gttaccggta cctcgccgaa tccatccgca agcatccgga tcaggccacg
      781 ctcaaggcca tgatggatgc cgccggcttc gcgcgcgtgg acgtgcgcaa cctctccgcc
      841 ggcatcgtcg cgatccatcg cggttaccgg atttgaccca ggcgttcggc gttcagccgc
      901 gaatttttct caatgccgca cccttgtgtg ccgcgacatg gtcggtcttg tcgctcttga
      961 tctcgtattg cggatcgtcc ggcgtcgcgt ggtgcgtgta tcccttgtag tcgaaatcgc
     1021 gcgtgtgcac cttgacgata gtgccgctga cgtatccggc ttccgaattc caggcgacgt
     1081 gatctccgat cttgaatttc tgggtcatgg ctgcgagcct gcatcgcgaa acaaatcgtc
     1141 gggcgcata
//
LOCUS       DASRVO010000308         1134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_61324, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000308 DASRVO010000000
VERSION     DASRVO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61324"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFF05_03790"
     CDS             complement(<1..488)
                     /locus_tag="VFF05_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HZW50957.1"
                     /translation="MNPAAVAGLSGVARRLVVEGTLGDEDARRAVDASLKQKIPLGSY
                     LIEHALANGAQVAMAASAEFGVPLFDVDALDINQNVVRLVNEKLITQHKALPLFKRGN
                     RLYVGVTDPTNLHALDEIKFQTNLTVEPVLVSEEQLGRVIERALNESGATIGDMGDDA
                     EGL"
     gene            665..>1134
                     /locus_tag="VFF05_03795"
     CDS             665..>1134
                     /locus_tag="VFF05_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW50958.1"
                     /translation="MISIVLPAKDEAAALESLLPRLRAAQPNAEILIVDDGSTDATGA
                     VCARHGVTVLRQPYSMGNGAAIKRGARGARGDILVFMDGDGQHDPADVARLVARLDEG
                     FDMAVGARDWGGQAGVGRGLANALYNWLASRMTGHTVADLTSGFRAVRADKFRE"
BASE COUNT          193 a          383 c          358 g          200 t
ORIGIN      
        1 agcccctcgg cgtcatcgcc catgtcgccg atcgtcgcgc ccgattcgtt cagcgcgcgc
       61 tcgatcacgc ggccaagctg ctcctcgctg accagcaccg gttccacggt gagattggtc
      121 tggaacttta tctcgtccag cgcatgcagg ttggtcggat cggtcacgcc cacgtacagg
      181 cggttgccgc gcttgaacag cggcagtgcc ttgtgctggg tgatcagctt ttcgttgacc
      241 aggcgcacga cattctgatt gatgtcgagc gcatccacgt cgaacaacgg cacgccgaat
      301 tcggcggacg cggccatcgc gacttgcgcg ccgttggcca aggcgtgttc gatcaggtag
      361 gagccaagcg gaatcttctg cttgagcgag gcgtcaacgg cccgacgcgc gtcttcgtcg
      421 cccaaggtgc cttcgaccac caggcgccgg gcaacgccgc tcagtccggc gaccgcggca
      481 ggattcatct gtgtcgccat aagaatcctt cgtaaaacct cgaaattaaa gccgaatgta
      541 ccgcaaaaac cgcccggatt gaatccccat ccgacgctgg catccccgct gtccagcggc
      601 tactcttggc cccatcggcc cggcttgcgg gttcgagtct actcctgaat tccggcggtc
      661 gagcgtgatc agcatcgtat taccggcaaa agacgaggcg gcggcgctgg aaagcctgct
      721 gccgcgcctg cgcgcggcac agccgaacgc cgaaatcctg atcgtggacg acggctcgac
      781 cgacgccacc ggcgcggttt gcgcccgtca cggcgtgacc gtactgcgcc agccgtattc
      841 gatgggcaat ggcgcggcga tcaagcgcgg cgcgcgcggg gcacgcggcg acatcctcgt
      901 attcatggat ggcgacggcc agcacgatcc cgccgacgtc gcccgtctcg ttgcaagact
      961 cgatgagggt ttcgacatgg ccgtcggcgc acgcgactgg ggcgggcagg ccggcgtcgg
     1021 tcgcggcctc gccaatgccc tctacaactg gctggcctcg cgcatgaccg ggcacaccgt
     1081 ggccgacctg accagcggct ttcgcgcggt gcgcgcggac aagttccgcg aatt
//
LOCUS       DASRVO010000309         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_372563, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000309 DASRVO010000000
VERSION     DASRVO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372563"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFF05_03800"
     CDS             <1..251
                     /locus_tag="VFF05_03800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZW50959.1"
                     /translation="DAFGEHASVTVLPVTSTRVPAPLLRVTLRPTAENGLQKTSQVMV
                     DKAITVRREKIGPAFGRIDADAMVEIERCLAVFLGIAK"
     gene            248..2638
                     /gene="xdhB"
                     /locus_tag="VFF05_03805"
     CDS             248..2638
                     /gene="xdhB"
                     /locus_tag="VFF05_03805"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HZW50960.1"
                     /translation="MTNDPIMRDPIPKSNPSVRIAKPHDSAHMHVSGEAIYGDDIAEP
                     RDLLHTYLRLADRAHARVTRMDLAKVRAAPGVAAVICAQDLAATTNDIGPVFPGDVVF
                     ADGEVHFHGQPLFAVAAESREVARRAALLAQIEYEDLPAILSIEDALAANAEVVPKKL
                     WTRGDPDGAIARAPHRLSGRIKMGGQEHFYLEGQNSMAIPQEDGQMLIYSSTQNPSEL
                     QHKAAEVLGVPANAVTAENRRMGGGFGGKETQAAWFAVIAAALAQRARRAVKCRLDRD
                     DDMTITGKRHAFLIDYKVGFDDDGRILGIDFLQAADCGYSPDLSHSVCDRAMAHADNA
                     YFLENVRVESRRCYTNKPSNTAFRGFGGPQGMVGIEEVIEQIARHLGLDPLVVRKINF
                     YGIDTRNVTPYGMTVEDNILDELVGELEQRVDYATRRRTIDAFNASHPYLKKGMALTP
                     VKFGISFTAKHLNQGGALVLVYLDGSVQLNHGGTEMGQGLHIKVAQVVAEVFGIDLDR
                     VQITPTNTGKVPNTSSTAASAGTDLNAAAAYNAAMKIIDRLKPFVRDRYHVASEHAIE
                     FRDNGVYANGARLASFVEVVKLAYMNRISLSAAGFYATPKLEVDELGRGRPFYYFAYG
                     AACTEVVVDTLTGEHRMTRVDIVHDVGKSLNPAIDMGQIEGGFIQGAGWLTSEELCWN
                     PQGRITTHAPSTYKIPACSDLPPHFDVRIFERGDNLEESVYRSKAVGEPPLMLAISVL
                     HALKDAVAAIGGHRRAAVLDAPATPEAVLMSVEEMKRATAPAPSRAHVEPEAAITA"
     gene            2652..>3053
                     /locus_tag="VFF05_03810"
     CDS             2652..>3053
                     /locus_tag="VFF05_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HZW50961.1"
                     /translation="MNSAIFWRAVLERLEAGTPLFVALVVANTRGSPGTLGARLLVDA
                     DGNTQGTIGGGIMEANLVRAARNGLRAGRDAPPALEQLEHRDKPGTAHPSGLICAGEQ
                     TNLNLWLQPSRDAATIRAFCTTLDAENGDAAT"
BASE COUNT          581 a         1015 c          965 g          492 t
ORIGIN      
        1 ccgacgcctt cggcgagcac gcgagcgtga cggtgttgcc ggtaacgagc acgcgggttc
       61 cggcaccgtt gctgcgtgtc acgctgcggc cgaccgcaga gaatggattg cagaaaactt
      121 cgcaggtgat ggtggacaaa gcgatcacgg tgcgccgcga aaaaatcggg cctgccttcg
      181 gccgcatcga cgcggatgcc atggtcgaga tcgagcgttg ccttgccgtg tttttgggga
      241 tagccaaatg acaaatgatc ccatcatgcg cgaccccatt ccgaagtcca atccgagcgt
      301 acgcatcgcc aagccgcacg acagcgcgca catgcacgtc agcggcgagg cgatttacgg
      361 cgacgacatc gccgagccgc gcgatctgct gcacacgtac ctgcgcctcg ctgatcgtgc
      421 gcacgcgcgc gtgacgcgca tggacttggc caaggtgcgc gccgcgcccg gcgtcgccgc
      481 cgtgatttgc gcgcaagacc ttgccgccac caccaacgac atcggcccgg tctttcccgg
      541 cgacgtggtg ttcgccgacg gcgaggtgca tttccacggc cagccgctgt tcgccgtcgc
      601 cgccgaatcg cgcgaagtcg cgcgtcgtgc cgcactgctg gcgcagatcg aatacgagga
      661 tttaccggcg atcctttcca tcgaggatgc gttggcggca aacgccgaag tggtgccgaa
      721 aaagttgtgg acgcgcggcg atccggacgg cgccatcgcg cgcgcgccgc atcgcctgag
      781 cggtcgcatc aagatgggcg gccaggaaca tttttatctg gaaggccaga acagcatggc
      841 gatcccgcag gaggatggcc agatgctgat ctacagttcc acacagaacc catccgagtt
      901 gcagcataag gccgccgaag tgctgggcgt gccggccaat gcggtgacgg ccgaaaaccg
      961 gcgcatgggc ggcgggttcg gcggcaagga gacgcaggcc gcgtggttcg ccgtcattgc
     1021 cgcagcgctg gcgcagcgcg cgcgccgcgc ggtgaaatgc cgcctcgacc gtgacgacga
     1081 catgaccatc accggcaagc gccacgcctt cctgatcgat tacaaagtcg gctttgacga
     1141 cgacggccgc atcctcggca tcgacttcct gcaggccgcc gactgcggct attcgccgga
     1201 tctttcgcat tcggtgtgcg atcgcgccat ggcccacgcc gacaacgcct acttcctgga
     1261 aaacgtgcgc gtcgaatcgc gccgctgcta caccaacaag ccatccaaca ccgcgttccg
     1321 cggcttcggc gggccacagg gcatggtcgg catcgaggaa gtgatcgaac aaatcgcgcg
     1381 tcatctcggg ctcgatccgc ttgttgtgcg caagatcaat ttctacggca tcgatacgcg
     1441 caacgtcacg ccctacggca tgacggtcga agacaacatc ctcgacgaac tggtcggtga
     1501 gttggaacag cgcgtcgatt acgccacgcg ccgtcgcacc atcgacgcgt tcaacgccag
     1561 ccatccatac ctgaagaaag gcatggcgct gacgccggtg aagttcggca tttcgttcac
     1621 cgccaagcat ctcaaccagg gcggtgcgct ggtgctggtt tatctcgacg gcagcgtgca
     1681 gctcaaccac ggcggcacgg agatggggca gggcctgcac atcaaggtcg cgcaagtcgt
     1741 ggccgaagtg ttcggcatcg acctcgaccg cgtccagatc acgccgacca acaccggcaa
     1801 ggtgcccaac acttcctcga ccgcggcgtc cgctggaacg gacttgaacg ccgcggcggc
     1861 ctacaacgcc gcgatgaaaa tcatcgaccg gctgaaaccc ttcgtgcgcg accgttacca
     1921 tgtcgctagc gagcacgcga tcgagttccg cgacaacggc gtgtacgcga atggtgcgcg
     1981 cttggcgagc ttcgtggaag tggtcaagct cgcctacatg aaccgcattt cactgtctgc
     2041 cgccggtttc tacgccacgc ccaaactcga agtcgacgaa ctcggccgcg gccggccgtt
     2101 ctattatttc gcctatggcg cggcctgcac cgaagtcgtc gtggatacgc tcaccggcga
     2161 gcaccgcatg acccgcgtcg acatcgtcca cgacgtcggc aagtcgctca atccggccat
     2221 cgacatgggc cagattgaag gcggattcat ccagggcgcc ggctggctga ccagcgagga
     2281 attgtgctgg aatccgcagg gccgcatcac cacgcacgcg ccgtcgacct acaagattcc
     2341 ggcgtgcagc gatctgccgc cgcacttcga cgtgcgcatc ttcgagcgcg gcgacaacct
     2401 cgaggaaagc gtgtatcgct ccaaggccgt cggcgaaccg ccgctgatgt tggccatttc
     2461 agtgctgcac gcgttgaaag atgccgtggc cgcaatcggc ggccatcgcc gcgccgccgt
     2521 gctcgacgcg ccagcgacgc ccgaggcagt gctgatgtcg gtcgaggaaa tgaaacgcgc
     2581 taccgcgcca gcgccgagtc gtgcgcacgt cgaacccgaa gcggcgatca cggcgtgagg
     2641 atggcgctgc ggtgaacagc gccatattct ggcgcgcagt acttgaacgg ctcgaagcgg
     2701 gcacgccgct gttcgtcgcg ctggtcgtcg ccaacacgcg cggctcgccg ggcacgctcg
     2761 gcgcgcgtct gctcgtcgat gccgacggca acacacaggg caccatcggc ggcggcatca
     2821 tggaagccaa tctcgtgcgc gcggcccgca atggtctgcg cgcgggcagg gacgcgccgc
     2881 cggcgctgga acaactcgaa catcgcgaca agccgggcac cgcgcatccg tcgggcctga
     2941 tctgcgcggg cgagcagacc aatctcaatt tgtggttgca gcccagccgc gacgccgcga
     3001 ccatccgagc tttttgcacc acgctcgacg ccgagaacgg cgatgcagca acg
//
LOCUS       DASRVO010000310         1012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_138749, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000310 DASRVO010000000
VERSION     DASRVO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138749"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1012)
                     /locus_tag="VFF05_03815"
     CDS             complement(<1..>1012)
                     /locus_tag="VFF05_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW50962.1"
                     /translation="GGCSSGGSVNAPASAAPGDVKLTAAQMQHVDLYTVAEAPFHKSI
                     RTAGTVDFDQERSTSLLAPISGPVMRLLVAPGDHVKKGQALAYVDSPDFAAAVGAFRK
                     ADAAARNARHIADVDKDLLAHKGVSDREAAQAQSDAIGAESDRDAARQALAALGVDEK
                     SITAIGNGKDVAFSGGAIRAPLAGVVVERLITPGQLLQAGTTPCFAIADLSRVWVNAQ
                     VFGGDVGQVNVGDSAEIDAGGTTLKGTVTNIADEVDPNTRSVTARVSVDNPHGALKKQ
                     MYVPVTIVARGESHGLLVPVSAILRDDENLPFVYVVQPDGGFARAHVRPGYRDGDRNV
                     VDD"
BASE COUNT          157 a          361 c          323 g          171 t
ORIGIN      
        1 atcatcgaca acgttgcggt cgccgtcgcg gtatcccggc ctcacgtgcg cgcgcgcaaa
       61 gcccccatcg ggctgcacga cgtaaacgaa cggcaggttc tcgtcgtcgc gcaggatcgc
      121 cgacacgggc accaacaacc catggctctc gccgcgcgcc acgatcgtca ccggcacgta
      181 catctgcttt ttcagcgcgc cgtgcggatt gtccacgctc acgcgcgccg tgaccgagcg
      241 cgtgttggga tcgacttcgt cggcaatgtt cgtcaccgtg cctttcagcg tcgtgccgcc
      301 ggcgtcgatt tcggcactgt caccgacgtt cacctggccg acgtcgccgc cgaacacctg
      361 cgcattcacc cacacccgcg acaggtcggc aatggcgaag cacggcgtgg ttccggcctg
      421 caacagctgg cccggcgtga tcaggcgctc cacgacgacg ccggcgagcg gcgcgcggat
      481 cgcgccgccg gaaaatgcga cgtctttgcc gttgccgatt gccgtgatgg atttctcgtc
      541 cacgccgagc gccgccaacg cctgacgcgc ggcgtcgcgg tcggactcag cgccgattgc
      601 atcggattgc gcttgtgccg cttcgcgatc cgatacgccc ttgtgcgcga gcaggtcctt
      661 gtccacgtcg gcgatgtggc gcgcgttgcg cgcggcggcg tcggccttgc ggaacgcgcc
      721 gaccgcggcg gcaaagtccg gcgaatccac gtaggccagc gcctggcctt tcttcacatg
      781 atcacccggc gcaaccagca gacgcatgac cggtccggaa atcggcgcga gcaggctggt
      841 ggaacgctcc tggtcgaaat cgaccgtgcc cgccgtgcgg atcgatttat ggaacggcgc
      901 ttcggccacg gtatacaagt cgacatgctg catctgcgcg gccgtgagct tcacgtcgcc
      961 gggcgcagcc gatgccggcg cgttcacgct gccgcccgac gaacatccgc cg
//
LOCUS       DASRVO010000311         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_191914, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000311 DASRVO010000000
VERSION     DASRVO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191914"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..151)
                     /gene="cydX"
                     /locus_tag="VFF05_03820"
     CDS             complement(50..151)
                     /gene="cydX"
                     /locus_tag="VFF05_03820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HZW50963.1"
                     /translation="MWYFTWILGLGFACAFAILNAMWVEIHETDNTP"
     gene            complement(153..1283)
                     /gene="cydB"
                     /locus_tag="VFF05_03825"
     CDS             complement(153..1283)
                     /gene="cydB"
                     /locus_tag="VFF05_03825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HZW50964.1"
                     /translation="MFDYETLRIIWWLLLGVLLIGFAIMDGLDLGVAALVPIVGRTDE
                     ERRVLLETVEPVWEGNQVWFVLGGGAVFAAWPVLYAASFSGLYLAMFLLLLAFILRPV
                     SFNFRNKHEHARWRGTWDILLAASGFIVMLVCGVAFGNLFLGFSFRFDDAARMDWQGS
                     FFSLFHPFALLAGLTSVCMLLAHGATYASLKTDEAVAPRTQAIARWASLAFVALYVLA
                     GAWLMTGAIPGMRVDSVDMNGESNPLLKHVVEAATWYGNFGAHPILFLAPALAIFAAL
                     GTFTLVRRHHVAALLTSSTAIAATILSVGFALFPFLMPSRLDPRSSLTVWDASSSHTT
                     LGIMLVCVFVFLPIVLAYTVWVYRVLRGRVTLEHIRRSHGVY"
     gene            complement(1294..>2321)
                     /locus_tag="VFF05_03830"
     CDS             complement(1294..>2321)
                     /locus_tag="VFF05_03830"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HZW50965.1"
                     /translation="FVHTVSAGYVLGAMFVLAISAWYLRKGRNVDIARRSFTVAASFG
                     LAASLSVVVLGDESGYNVSENQKMKLAAIEAMWETEPPPASFTVFGLPDTKNHATHYA
                     LRIPWVMGLIGTRSIDTPMPGIAELVADAAQRVRRGMVAYDALLKVRAHPDDAAARAE
                     LEAHASDLGYALLLKKFIEDPRKATDADIEKAANSTVPNVPVLFWSFRVMVACGFYFI
                     GLFAAAFWFASRRTIEQHPRFLRLALWSLPLPWLAAELGWIVAEYGRQPWAIEGVLPT
                     SLGVSSLSALQVSLSLGGFVLFYSTLAVIDAWLMTKFARKGPDGLGMWPHGPAKAERQ
                     QEVRATA"
BASE COUNT          479 a          740 c          751 g          351 t
ORIGIN      
        1 cagcggccgg aagaaatccg gggcaaatca cttcctgact tgcggaatgt catggtgtgt
       61 tgtcggtctc atgtatctcg acccacattg cgttgaggat ggcgaaggcg caggcgaagc
      121 cgagcccgag tatccaggtg aagtaccaca tgtcagtaga ctccgtgcga gcggcggatg
      181 tgttcgagcg tgacgcggcc gcgcagcacg cggtacaccc acacggtgta ggcgagtacg
      241 atcggcagga acacgaacac gcagacgagc atgatgccga gcgtggtgtg acttgacgag
      301 gcgtcccaga ccgtcaacga ggagcgtgga tccaggcgcg acggcatcag gaacggaaac
      361 aacgcgaagc ccacggacag gatcgtcgcc gcgatggcgg tgctggaggt caacaacgct
      421 gcgacgtggt gcctgcgcac cagcgtgaac gtgccgagtg cggcgaagat cgccagtgcc
      481 ggcgcaagga acaggatcgg atgagcgcca aagttgccgt accaagtcgc cgcctcgacc
      541 acatgcttga gcagcggatt cgactcgccg ttcatgtcga cgctgtccac tcgcattccg
      601 ggaatcgctc cggtcattag ccaagcgcct gccaggacat agagcgcgac gaacgcgagc
      661 gacgcccagc gcgcgatggc ctgcgtgcgc ggcgctacgg cttcatccgt cttcagcgat
      721 gcgtacgtcg cgccgtgtgc cagcaacatg caaacgctgg tgagcccggc caacagtgcg
      781 aaagggtgga acagcgagaa gaagctgcct tgccagtcca tgcgcgccgc gtcgtcgaag
      841 cgaaacgaga atccgaggaa cagattgccg aacgccacac cacacaccag catcacgatg
      901 aatccggatg cggcgagcag aatgtcccag gtgccgcgcc agcgcgcgtg ctcgtgcttg
      961 ttgcgaaagt tgaacgacac tggccgcagg atgaaggcga gcagcagcag gaacatcgcc
     1021 aggtacagcc cggaaaacga cgccgcgtac agcaccggcc aagcggcgaa cacggcgcct
     1081 ccgccgagca cgaaccagac ctggttgcct tcccacaccg gttccaccgt ttccagcagg
     1141 acgcggcgct cctcgtcggt gcgccctacg attggcacga gtgcggcgac gccgagatcg
     1201 aggccgtcca tgatcgcaaa gccgatcagc agcacgccga gcagcagcca ccagatgatt
     1261 cgcagggttt cgtagtcgaa catggcagtc tcctcaggcc gtcgcccgaa cttcttgctg
     1321 ccgctcggct ttcgccggac cgtgcggcca catgccgagt ccgtccggac ccttgcgtgc
     1381 gaacttcgtc atcagccagg cgtcgatgac ggcaagtgtg ctgtagaaca gtacgaagcc
     1441 gccgagcgat aacgacacct gcagcgccga cagcgacgac acgccgaggc tggtgggcag
     1501 cacgccttcg atcgcccacg gctggcggcc gtattcggca acgatccagc caagttcggc
     1561 agcgagccac ggcagcggaa ggctccacaa tgccaggcgc aggaaacgcg gatgctgttc
     1621 gatcgtgcgc cgcgaggcaa accagaacgc cgcagcgaaa agaccgatga aatagaaccc
     1681 gcacgccacc atcacgcgga acgaccagaa cagcaccggc acgttcggta cggtactgtt
     1741 cgctgctttc tcgatgtcgg cgtcggtggc cttgcgcgga tcctcgatga atttctttag
     1801 cagcagcgcg tagccgagat ccgacgcatg cgcttcgagt tccgcgcgcg cggccgcgtc
     1861 gtcgggatgc gcgcggacct tcagcagtgc gtcgtaggcg accatgccgc gccgcacgcg
     1921 ctgcgccgcg tcggcgacga gctcggcgat gcccggcatc ggcgtgtcga tcgagcgcgt
     1981 gccgatgaga cccatcaccc acggaatgcg cagcgcgtag tgcgtggcgt ggttcttggt
     2041 atcgggcaag ccgaacacgg tgaaagacgc gggcggcggt tcggtttccc acatcgcctc
     2101 gatcgcggcg agcttcatct tctggttttc cgacacgttg tagccggatt cgtcgccgag
     2161 cacgacgacg ctgagcgatg cggcgagccc gaagctcgcg gcgaccgtaa acgagcggcg
     2221 cgcgatgtcg acgttgcggc ccttgcgcag ataccaggcg ctgatcgcca gcacgaacat
     2281 cgcgccgagc acgtatccag cgctcaccgt gtgcacgaac t
//
LOCUS       DASRVO010000312         2291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_372743, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000312 DASRVO010000000
VERSION     DASRVO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372743"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..572
                     /locus_tag="VFF05_03835"
     CDS             219..572
                     /locus_tag="VFF05_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZW50966.1"
                     /translation="MKSGTTAIPPEHAAIVRVIRSIPRGRVTSYGEIAARAGLPGRAR
                     MIGRILGAYSALKLPWQRVLRSDGRIAFPAGSRGHREQRALLVDEGVVVRNGRVDLAR
                     FGWQRDLDAQLWAPK"
     gene            591..1226
                     /locus_tag="VFF05_03840"
     CDS             591..1226
                     /locus_tag="VFF05_03840"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZW50967.1"
                     /translation="MPNFPPVTRVLLIANVAVFLLQQAGADATIVAHFALWPLGEQQL
                     ARMADGSLIAPGFEPWQLVTYSFLHGGVPHIAFNMLALWMFGGPVENALGARRYAFYY
                     FACVLGAAFAQLATIALFSPQDFYPTLGASGGVFGLLLAFAVLYPQAKVFFFLIPVPI
                     PARIAVVGYMILELFLGVTGTQAGIAHFAHLGGAVVGFGLIQYWRGRVRRR"
     gene            complement(1288..>2291)
                     /locus_tag="VFF05_03845"
     CDS             complement(1288..>2291)
                     /locus_tag="VFF05_03845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HZW50968.1"
                     /translation="GAVNRSMGQGLGQLYVAKYFPPEAKARAAELVDNVRNALKNRIE
                     HLDWMSDATKAKAIAKWNTFLPKIGYPDKWRSWDGLTVTADDYYGNLQAARKFNYDYD
                     ITKIGKPTDRMEWFMTPQTVNAYYNPTDNTINFPAAILQPPFFYADGDDAINYGGIGA
                     VIGHEASHGFDDQGSQFDGDGNNVNWWTKEDRAKFDARTQKLVDQFDTYTPLANHPDK
                     HVNGRLTLGENIGDLGGLNAAYDALQMALAKNPKEAAEKIDGYTQDQRFFLNWARVWR
                     GSTREQRQLVLLNADPHSPTQFRAIGAPSNMPAFAQAFSCKTGDAMVRNGDKQVKIW"
BASE COUNT          325 a          698 c          757 g          511 t
ORIGIN      
        1 tggaaacgcg caggaacttg cggccatcct cgccctcgat ttcggcgagg tagatgccct
       61 gttggcgcat gccggcgccg atggcgagat cgaaactgat caggtatgga tcgttgatgc
      121 gcaacgcatg cctggcctgg atgctggagc ggatttcctc gaacgaatgc atgatgtgtt
      181 ccttgtgtac ttgccgcgta tgctaacgtg tcgccgcgat gaagtcaggc acgaccgcca
      241 taccgcccga gcatgccgcc attgtgcgcg tgatccgctc gattccgcgc ggccgggtga
      301 cgagctacgg cgagatcgcg gcgcgcgcgg gcctgcccgg tcgcgcgcgt atgatcggac
      361 gcatcctcgg cgcgtactcc gcgctgaaat tgccatggca gcgcgtgttg cgctcggatg
      421 gacgcatcgc gtttcccgcc ggcagccgtg gccatcgcga gcaacgggca ttgctggtcg
      481 acgagggcgt ggtcgtgcgc aacggtcgcg tcgatctggc acgcttcggc tggcagcgcg
      541 atctggatgc gcaactgtgg gcgccaaaat gacaatggat tctttctgac atgccgaatt
      601 tcccacccgt cacccgtgtc ttgctgatcg ccaatgtcgc ggttttcctt ttgcagcagg
      661 ccggcgccga cgccaccatc gtcgcgcact tcgcgctgtg gccgctcggc gagcagcagc
      721 ttgcgcgcat ggccgacggc agtttgatcg cgcccggctt cgagccgtgg caactcgtca
      781 cctacagttt cctgcacggt ggcgtgccgc atatcgcgtt caacatgctt gcgctgtgga
      841 tgttcggcgg tccggtcgag aacgcgctcg gtgcgcgccg gtatgcgttt tattatttcg
      901 cctgcgtgct cggcgcggcg ttcgcgcagt tggcgacgat cgcattgttc tcgccgcagg
      961 atttctatcc aacccttggc gcctcgggcg gcgttttcgg actgctgctc gccttcgccg
     1021 tgttgtatcc gcaggcgaaa gtgttcttct tcctgatccc ggtgccgatt ccggcgcgca
     1081 tcgccgtggt cggttacatg attctggaac tgtttctcgg cgtcaccggc acgcaggccg
     1141 gcatcgcgca ttttgcgcac ctgggcggcg ccgtggtggg cttcggcttg atccagtatt
     1201 ggcggggtcg ggtgcgcagg cgttgatgcc gcaccaatga aaacgccgcg gaggccgcgg
     1261 cgttttcgct tcacctgaag attgcggtta ccagatcttc acctgcttgt cgccgttgcg
     1321 caccatcgca tcgccggtct tgcacgagaa cgcctgggcg aacgcgggca tgttcgacgg
     1381 cgcgccgatg gcgcggaact gcgttggcga gtgcggatcg gcgttgagca gcaccagctg
     1441 gcgctgctcg cgcgtggagc cgcgccacac ccgagcccag ttcaggaaga agcgctggtc
     1501 ctgggtgtag ccgtcgattt tctccgcggc ttccttcgga ttcttcgcca gcgccatctg
     1561 cagcgcgtca taggccgcat tgaggccgcc gaggtcgccg atgttctcgc ccagggtcag
     1621 gcggccgttg acgtgcttgt ccggatggtt cgccagcggc gtgtaggtgt cgaactggtc
     1681 gaccagtttt tgcgtgcgtg cgtcgaactt ggcgcggtct tccttcgtcc accagttgac
     1741 attattgccg tcgccgtcga actggctgcc ctggtcgtcg aagccgtggc tggcttcatg
     1801 gccgatcacc gcgccgatgc cgccgtagtt gatcgcgtcg tcgccgtcgg catagaagaa
     1861 cggtggttgc aggatcgccg ccgggaagtt gatcgtgttg tcggtcggat tgtagtaggc
     1921 attgaccgtc tgcggcgtca tgaaccattc catgcggtcg gtcggcttgc cgatcttggt
     1981 gatgtcatag tcgtagttga acttgcgcgc cgcctgcagg ttgccgtagt aatcgtccgc
     2041 cgtgacggtc aggccgtccc acgaacgcca tttgtccggg tagccgatct tgggcaggaa
     2101 ggtgttccac ttggcgatgg ccttggcctt ggtggcgtcg ctcatccagt ccaggtgctc
     2161 gatgcggttt ttcagcgcat tgcgcacgtt gtcgaccagt tcggcggcgc gcgccttggc
     2221 ttcgggcggg aaatacttgg ccacgtaaag ctggccgagg ccctggccca tcgaacgatt
     2281 gacggcgcca a
//
LOCUS       DASRVO010000313         1917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_346608, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000313 DASRVO010000000
VERSION     DASRVO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346608"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1005)
                     /locus_tag="VFF05_03850"
     CDS             complement(79..1005)
                     /locus_tag="VFF05_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZW50969.1"
                     /translation="MRWALLLLFALALAAPAAAATLRVGPHEAYKTPSAAAAVAHNGD
                     HIEIDPGRYVDCAVWRADNLVIEGSGPSVVIADKSCAAKGLFIIDGNNTTVRNLTLAH
                     ARVRDMNGSGIRLEHGNLTVDGVTFIANEMGILGGFPGATVVVRNSSFIDNFKHGSHR
                     CISWCDHAVYIDRAEFVRIEHSRFIGTRQGHSIKSRAAGTEVIGCTIADGPDGTSSFL
                     IDAPNGGTLVVRGNTLEKGPHSENRTTAIAIGEEGVTHPTPRIIVADNSFRNDSDHET
                     ALVWNRTSTPAQLRGNSFSGPVIPLVGPGSAR"
     gene            complement(1035..>1917)
                     /gene="rpoD"
                     /locus_tag="VFF05_03855"
     CDS             complement(1035..>1917)
                     /gene="rpoD"
                     /locus_tag="VFF05_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZW50970.1"
                     /translation="LPGRGWAKLAERHATEVAKHRAAVAQLAQDAKLPIIEFRRVVQT
                     VQRGEREASRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYR
                     RGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLVRTSRQMLHEIGREPTP
                     EELAEKLQMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDFIEDKNAVLPLDAAIQA
                     NLRETTTRVLASLTAREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALR
                     KLKHPSRSRKLRSFLDT"
BASE COUNT          297 a          606 c          625 g          389 t
ORIGIN      
        1 ccagctgaac tacgggcccg cacgcgcgat cataacgtcc gccgagtgcg ctgtcgcctc
       61 cgacgccgga tgcgcagatc acctcgcgct gccggggccg acgagcggga tcaccggccc
      121 cgaaaagctg ttgccgcgga gttgcgccgg ggtgctggtg cggttccaca caagggcggt
      181 ttcgtggtcg ctgtcattgc gaaaggaatt gtcggcgaca atgatgcgcg gggtcgggtg
      241 cgtcacccct tcctcaccga tcgcgatcgc ggttgtgcga ttttccgagt gtggtccctt
      301 ttccaacgta ttgccgcgca caacgagcgt gccgccgttg ggcgcgtcga tcagaaagct
      361 ggaggtgccg tcgggaccgt cggcgatcgt gcagccgatg acttcggtgc cggcggcgcg
      421 cgacttgatc gaatgaccct ggcgggtgcc gataaatcgc gagtgttcga tcctgacaaa
      481 ttccgcccgg tcaatataga ccgcatggtc gcaccagctt atgcaacggt ggctgccgtg
      541 cttgaagttg tcgatgaagc tgctgttgcg cacgacgacg gtcgcgccgg ggaagccgcc
      601 caaaataccc atctcgttgg cgatgaaggt gacgccgtcg accgtcagat tgccgtgttc
      661 gaggcggatg ccggagccgt tcatgtcgcg tacgcgggca tgcgccagcg tcaggttgcg
      721 gacggtcgta ttgttgccgt caatgatgaa caggcctttt gccgcgcagc ttttgtcggc
      781 gatgacaacg ctggggccgc tgccctcgat gacgagattg tcggcgcgcc acaccgcgca
      841 atcgacgtag cgcccggggt cgatctcgat atggtcaccg ttgtgcgcaa ccgcggccgc
      901 cgccgagggt gtcttatagg cttcgtgcgg ccccacccgc agcgtcgccg cggcagccgg
      961 ggccgccagc gccaatgcga acagcagcag cagcgcccat cgcatcgccg ccaccgcttt
     1021 cgcggcgctc tgccttaggt atcgagaaag cttctgagtt tacgcgagcg gcttggatgc
     1081 ttcagtttgc gcagcgcctt cgcttcgatc tggcggatgc gttcgcgcgt caccgagaat
     1141 tgctggccaa cctcttccag cgtgtgatcg gtgttcatgc cgatgccgaa gcgcatccgc
     1201 aagacgcgct cctcgcgcgc cgtcaacgac gcgaggacgc gcgtcgtcgt ctcgcgcaga
     1261 ttggcctgga tcgccgcgtc caacggcaag accgcgttct tgtcctcgat gaaatcgccg
     1321 aggtgcgaat cctcttcgtc gccgatcgga gtttcgaggc tgatcggctc cttggcgatc
     1381 ttcaggacct tgcgcacctt ctcgagcggc atctggagct tctcggccag ctcttccggt
     1441 gtcggctcgc gcccgatctc gtgcagcatc tggcgcgagg tgcggaccag cttgttgatc
     1501 gtctcgatca tatgcaccgg gatgcggatc gtgcgcgcct ggtcggcgat cgagcgggtg
     1561 atcgcctggc ggatccacca cgtcgcatag gtcgagaact tgtagccgcg gcggtactcg
     1621 aatttatcga ccgccttcat cagcccgata ttgccttcct ggatcaggtc gaggaattgc
     1681 aggccacgat tggtgtattt cttggcgatc gagatgacga gccgcaggtt cgcctcgacc
     1741 atctccttct ttgcacgact cgcctcgcgt tcgccgcgct gcacggtctg cacgacgcgg
     1801 cggaactcga tgatcggcag ctttgcatcc tgcgcgagct gcgcgaccgc tgcgcggtgc
     1861 ttggcgacct cggtggcgtg gcgttcggcg agcttggccc aaccgcggcc gggcagc
//
LOCUS       DASRVO010000314         1945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_191980, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000314 DASRVO010000000
VERSION     DASRVO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191980"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VFF05_03860"
     CDS             complement(<1..704)
                     /locus_tag="VFF05_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZW50971.1"
                     /translation="MKRIAIVLSLAVAMSGCATIKGWFNDTKKENIQPPTPLTQFAPT
                     LNLQKLWDTHPTSGAGESGARVAPAYADGKLFVAGVDGDIAALDAASGRTIWSKRFGQ
                     RHGFILHHGQNSVRWAGGPAVDGNLLVVGGLEGAVHALDAGSGAERWQAQLSSEVIAA
                     PAIADGIVVVRTDDGRLFGLNAADGSRHWVYDRSAVPALSLRGNAAPRIADGVVYAGE
                     DSGKLVALRLSDGNVM"
     gene            complement(701..1351)
                     /locus_tag="VFF05_03865"
     CDS             complement(701..1351)
                     /locus_tag="VFF05_03865"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW50972.1"
                     /translation="MAFEVLDEHEQGELVQKWLRENALSILIGVGLGLVLLFGWQQWR
                     SHRNAHRLDAATQYQVFRDDLTKKDVDGAKAIAAKLQSDYADTPYAVLAAMLLASDDA
                     ARGDEAAANKALQDAYVHAGIGALKTLAGLNLARSEIAQKKAQDALTLLDSLPEEGYA
                     ALREELRGDALSALGRSADARRAYTDALTHLDANAANRTFVQMKLDNLAGVEKKGS"
     gene            complement(1507..>1945)
                     /locus_tag="VFF05_03870"
     CDS             complement(1507..>1945)
                     /locus_tag="VFF05_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZW50973.1"
                     /translation="TGPTAFAATDVAQAAPKPASVPASTKPEEPPLVASMTPFPAAAE
                     SKPAVVSAPVAEAANRVHLSLSQASWVEIVDTDGTKLEYGLLPAGSERDYASNRALDI
                     RIGNADGAAVSIDGKAQDLTSFRHANVAHLRIADGTASTARGG"
BASE COUNT          318 a          629 c          629 g          369 t
ORIGIN      
        1 cacatgacat tgccatcgga caggcgcagc gcgacaagtt tgccgctgtc ctcgcccgca
       61 tacacgacac cgtcggcgat gcgcggcgcg gcattgccgc gcaagctcag cgccggcacc
      121 gcggagcggt cgtagaccca atgccgcgat ccatcggcgg cattcaggcc gaacaggcgg
      181 ccatcgtcgg tgcgcaccac gacgatgccg tcggcaatcg caggcgccgc gatgacttcg
      241 gaggaaagct gcgcctgcca gcgctccgct ccactgccgg catccagcgc atgcaccgca
      301 ccttcgagtc ctccgaccac gagcaggttg ccgtcgacgg ccgggccgcc ggcccagcgt
      361 acggagttct ggccatgatg caggatgaag ccgtggcgct ggccgaaacg cttgctccag
      421 atagtgcggc cggatgcggc atcgagcgcg gcaatgtcgc catcgacgcc ggcgacgaag
      481 agcttgccgt cggcgtaggc aggcgcaacg cgcgcgccgc tctcgccagc gccgctggtc
      541 ggatgcgtat cccacaattt ctgcaagttc agcgtcggtg cgaactgcgt cagcggggtg
      601 ggcggctgga tgttttcctt cttggtatcg ttgaaccaac ctttgatggt ggcgcagccg
      661 ctcatcgcga cggccagcga caacacgatc gcgatgcgct tcatgagcct ttcttctcca
      721 cgccggcgag gttgtcgagc ttcatttgca cgaaggtgcg gttggccgcg ttcgcatcga
      781 gatgggtcag cgcgtccgtg taggctctgc gcgcatcggc gctgcggccg agtgcggaca
      841 gcgcatcgcc gcgcaattct tcgcgcagcg cggcatagcc ctcttcgggc aggctgtcga
      901 gcagggtcag tgcatcctgc gcctttttct gcgcaatctc ggaacgcgcc aggttcaatc
      961 ctgccaaggt tttcaacgcg ccgatcccgg catggacgta ggcatcctgc aaggccttgt
     1021 tcgccgcggc ttcatcgccg cgcgccgcat cgtcgctggc gagcagcatc gcggcgagaa
     1081 cggcatacgg cgtatccgca taatcgcttt gcagcttcgc ggcgatggcc ttggcgccgt
     1141 cgacgtcttt tttcgtcaga tcgtcccgga acacctggta ttgcgtggcg gcatccagac
     1201 gatgcgcatt gcggtggctg cgccactgct gccagccgaa gagcaggacc aatccgaggc
     1261 caacgccgat cagaatggac agagcgtttt cgcgcagcca cttttgtacc aattcgcctt
     1321 gttcgtgttc atccaaaact tcgaaagcca ttgcgatatc cagatatgga attgaattcg
     1381 tctcgcacgc ggcggcgcca cgcgcgaggg tgcgtagggt agcaggaacg caggcattgc
     1441 gggttcaaac cgcgttagcg actcgcacga tcgcgagcgc ggttactcgc cggttcatgc
     1501 ggccgttcaa ccgccgcgtg ccgtactcgc cgtgccgtcc gcgatcctca gatgcgcgac
     1561 attcgcatgg cggaacgacg tcagatcctg cgccttgccg tcgatggata cggctgctcc
     1621 gtcggcgttg ccgatgcgga tatccagggc ccgattgctg gcgtagtcgc gttcgctgcc
     1681 ggcaggcagc aatccatatt cgagctttgt gccgtcggtg tcgacgattt ccacccaact
     1741 ggcctgactc agactcaggt gcacgcggtt ggctgcctcc gcgacgggcg cggaaacgac
     1801 ggccggtttg ctttcggctg cggccgggaa cggcgtcatc gacgcaacca gagggggttc
     1861 ttcgggtttt gtcgacgcgg gaaccgacgc cggtttcggg gccgcttggg caacgtccgt
     1921 cgccgcgaat gccgtggggc ctgtc
//
LOCUS       DASRVO010000315         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_217581, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000315 DASRVO010000000
VERSION     DASRVO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217581"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VFF05_03875"
     CDS             complement(<1..609)
                     /locus_tag="VFF05_03875"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HZW50974.1"
                     /translation="MTTATAREAAINDGDREGALHRYRRVRSRTEKLAAPLSAEDQTV
                     QSMSDVSPTKWHRAHTSWFFEQFLLTPFAPSYRSLDRAYGYLFNSYYEGAGARHPRPE
                     RGLLSRPGIDEIARYRAHVDAGMEALLARAGENQWREIAPLLALGLNHEEQHQELILM
                     DIKHVLGLNPLDPAYAKPPPAMHRPAAPLEFREFGEGLREIGY"
     gene            767..>1053
                     /locus_tag="VFF05_03880"
     CDS             767..>1053
                     /locus_tag="VFF05_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50975.1"
                     /translation="MALMLALAPSLAHARAGGSYRAGGGSSFMSQGSRGFGTYRPNGG
                     APIARSITPQSGTSTPYGGYPSGYGYGYGHSFFGGLFGGFFGSWLGSMLFP"
BASE COUNT          183 a          333 c          332 g          205 t
ORIGIN      
        1 atacccgatc tcgcgcaagc cctcgccgaa ttcgcggaac tcgagcggcg cggctggacg
       61 atgcatcgcc ggcggcggtt tggcataagc gggatcgagc gggttcagcc ccagcacatg
      121 cttgatgtcc atcaggatca attcctgatg ctgctcttcg tgattgagcc ccagcgccaa
      181 aagtggcgcg atctcgcgcc actgattttc tccggcacgc gcgagcagcg cctccatccc
      241 cgcatcgaca tgcgcgcgat agcgcgcgat ctcgtcgatg ccgggccgcg acaagagtcc
      301 gcgctcgggc cgcggatgac gcgcgcccgc tccttcgtaa taggaattga agagataccc
      361 ataggcgcga tcgagggacc gataggatgg tgcaaacggt gtcagcagaa attgctcgaa
      421 gaaccagctg gtgtgcgcgc ggtgccattt ggtcggcgag acatcgctca tcgactgcac
      481 ggtctggtcc tcggcggaaa gcggcgccgc caatttctcc gtgcgtgagc gcacgcggcg
      541 gtaacggtgc aatgcacctt cgcggtcgcc atcgttgatg gcggcttctc gtgccgtcgc
      601 cgtcgtcacc gtgccctccc accgtggttg cccagggtgc cagataacag ctaatttctc
      661 cgcgccatcc ttgcggaacg gggcgcggct gacgagatat tagcgcgatc cataggatca
      721 caagaggaat ttggcaatga taaggcgaag ctggttcgtt gtgctgatgg cgctcatgct
      781 cgcactcgca ccgagcctcg cgcatgcgcg cgccggcggc agctatcgcg ccggcggcgg
      841 atcgagcttc atgagtcaag gcagccgcgg cttcggcacc tatcgtccca atggcggcgc
      901 gccgatcgcg cgcagcatca cgccgcaaag cggcaccagc acgccttatg gcggatatcc
      961 ctccggctat ggctatggtt acggccattc tttcttcggc gggctgttcg gcggtttctt
     1021 cggttcatgg ctcggcagca tgctgtttcc gca
//
LOCUS       DASRVO010000316         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_269642, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000316 DASRVO010000000
VERSION     DASRVO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269642"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VFF05_03885"
     CDS             <1..147
                     /locus_tag="VFF05_03885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010382291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZW50976.1"
                     /translation="VKVGERVKRGQMIGLAGMTGRATGPHVHWGFNWFDVRLDPALLP
                     KPQD"
     gene            complement(138..1073)
                     /gene="folE2"
                     /locus_tag="VFF05_03890"
     CDS             complement(138..1073)
                     /gene="folE2"
                     /locus_tag="VFF05_03890"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HZW50977.1"
                     /translation="MHSNSIHSLPDVASDAQTDAAGALDWVGMENIALPIRLIGEDGA
                     TAQSAARVSAFVNLADPAARGIHMSRLYLLLDRALGAEPLTPASLRRLLHDFLASHAG
                     LSDRAMLRIAFEHLLRRPALVSDNSGWKAYPVEISATLVPGHFALELQVDVQYSSTCP
                     ASAALARQVIQSRFTADFARGKALDHMAVERWLGSEQGISATPHSQRSTARVRVRLAP
                     GFDLPIAELVDAIEVALATPVQTAVKREDEQAFARLNGENSMFCEDAARRIRGALDAD
                     ERIADFHIRVAHHESLHAHDAVAVVTKRVANGFQS"
     gene            complement(1106..1954)
                     /locus_tag="VFF05_03895"
     CDS             complement(1106..1954)
                     /locus_tag="VFF05_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HZW50978.1"
                     /translation="MADASSTFASFEQKWLMANPENAIVAVFLPSVQRRFAGAFGSLI
                     HELSSAAFRIREPQVAATKLAWWRQELADAAFGKPSHPVTRALFDCAGVRESDPALWP
                     ALADGALAQLDAPGAGTLAALIEQLDPFFGAVARAENALFSTGECNIDADAALWTLSH
                     LLRALPHLADGEAHLPLPLGLLARHELTRAELAYESPQRNMLIKDFLDELVLESNGAL
                     GVSSVRSLPLRVRAALDRRRIESALRVVDPLRWLSTHPHAGRGRTLWTTWHEARAARR
                     AQAGLR"
     gene            complement(1958..2620)
                     /gene="gph"
                     /locus_tag="VFF05_03900"
     CDS             complement(1958..2620)
                     /gene="gph"
                     /locus_tag="VFF05_03900"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207626.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HZW50979.1"
                     /translation="MRQSTPAAVFFDLDGTLVDSAPDMVEAMRRLRLEEGESPDGAES
                     IGAVVSKGGRAMIRRGFPAADEARVEVLLPRFLDLYAQAIATHSALFPGMGAVLDGLE
                     MRGTHWGIVTNKPGTLARALLAALRLDARCAALVTGDCLPLKKPDPAPILRACEIVGV
                     SAARCIYVGDDLRDIEAGHRAGMRTIAAGWGYLDGEDPRSWGADCVVDSPAQLLPAFA
                     LD"
     gene            complement(2620..3324)
                     /gene="ubiG"
                     /locus_tag="VFF05_03905"
     CDS             complement(2620..3324)
                     /gene="ubiG"
                     /locus_tag="VFF05_03905"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HZW50980.1"
                     /translation="MNATNVSVAETAKFDALAARWWDINGESRPLHDLNPARLNYIAE
                     RVALKGARVLDVGCGGGILSESLARAGADVTGIDLASRVLEVARLHLHESGVRVEYRE
                     IAVEALAAQMPGAFDAVTCMELLEHVPDPGSVIQACATLLKPGGRLFVSTLNRTPLAF
                     GAAIAGAEYALNLLPRGTHHYAQFIRPSELATMLREHGLELEDLSGLAYNPFTRRAAI
                     VKSTQVNYLACTRKPV"
     gene            complement(3326..>3544)
                     /locus_tag="VFF05_03910"
     CDS             complement(3326..>3544)
                     /locus_tag="VFF05_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207629.1"
                     /note="Catalyzes the hydrolytic cleavage of a
                     carbon-halogen bond in N-ethylammeline; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HZW50981.1"
                     /translation="KQADLVAVRLDALETQPLYNAISQIVYATGRHQVGDVWIAGVRK
                     VENSALCAIDTDALRAKTQAWRARIAAN"
BASE COUNT          647 a         1165 c         1145 g          587 t
ORIGIN      
        1 gtgaaggttg gcgagcgcgt gaaacgcggc cagatgatcg gtctggccgg catgaccgga
       61 cgcgccaccg gcccgcacgt gcactgggga ttcaactggt tcgacgtgcg gctggatccg
      121 gcgctattgc ccaagcctca ggattgaaat ccgtttgcca cgcgcttcgt gaccaccgcc
      181 accgcatcgt gggcgtgcag gctctcgtgg tgcgcgacgc ggatatggaa atcggcgatg
      241 cgctcgtccg catccaatgc gccgcggata cgccgcgcgg catcttcgca gaacatcgaa
      301 ttctcgccgt tcaggcgtgc gaaagcctgt tcgtcttcgc gcttgaccgc ggtctgcacc
      361 ggcgtagcaa gcgcaacttc aattgcgtcg acgagttcgg cgataggcag atcgaatccc
      421 ggcgccagcc gcacgcgcac ccgcgccgtg ctgcgctggc tgtgcggcgt ggcgctgatg
      481 ccctgctcgc tgccgagcca gcgttccacg gccatatgat ccagcgcctt gccacgggcg
      541 aagtccgctg tgaaacggct ctggatcact tgccgtgcca gtgcggctga agccgggcac
      601 gtactcgaat actgcacgtc cacctgcaat tccagcgcaa aatgtcccgg caccagcgtc
      661 gcggatattt caaccggata agctttccag ccgctgttgt cgctcaccag cgcgggtcgg
      721 cgcagcaaat gttcgaaggc gatgcgcaac atcgcgcggt cggacaaacc cgcatgcgaa
      781 gcgaggaaat cgtgcagcag gcgacgcagc gaagcgggtg tcagcggttc ggcgcccagt
      841 gcgcgatcca gcagcaggta gagccgcgac atgtggatgc cgcgtgccgc aggatcggcg
      901 agattcacga acgcggacac gcgtgccgcg gactgcgcgg tcgcgccatc ctcgccgatc
      961 aggcgtatcg gcaaggcgat attttccatg ccgacccaat ccagcgcgcc ggcagcatcg
     1021 gtttgtgcgt cgctggcgac gtcgggaagg gaatgaatcg aattcgagtg catgcgaacg
     1081 tgactgcatc ggggaaccgt ggattttagc gcaagccggc ctgcgcccgc cttgcggcac
     1141 gagcctcatg ccatgtcgtc cacagcgtcc gcccccgccc tgcatgcgga tgcgtgctca
     1201 gccatcgcag gggatcgact acgcgcaagg cggactcgat gcgccggcgg tcgagcgcgg
     1261 cgcgcacgcg cagcggcagc gagcgcaccg aactcacgcc gagcgcgcca ttgctttcca
     1321 gcacgagttc gtcgagaaaa tccttgatca gcatgttccg ctgcggcgat tcgtaggcaa
     1381 gttccgcacg cgtcaactcg tggcgtgcca gcaaaccgag cggcagcggc aggtgcgctt
     1441 cgccatcggc gagatgtggc agtgcacgca gcaaatgcga caatgtccac aatgctgcat
     1501 cggcatcgat attgcattca cctgtgctaa atagcgcgtt ttccgcacgc gcgacggcgc
     1561 cgaaaaacgg atcgagctgt tcgatcaatg cagccagcgt gccggcgccg ggcgcatcga
     1621 gctgcgccag cgctccgtcg gcaagagccg gccacagcgc tggatcggat tcgcgcacgc
     1681 cggcgcaatc gaacaaggct cgcgtgaccg gatgactggg tttgccgaac gcggcgtcgg
     1741 ccagttcctg ccgccaccac gcgagtttcg tcgccgcgac ctgcggttcg cgaatgcgga
     1801 atgcggcaga agacaattca tggatcagcg aaccgaacgc gccggcgaac ctgcgctgca
     1861 ccgatggcag gaacaccgcg acgatggcat tctcgggatt ggccatcagc catttctgct
     1921 cgaaactcgc gaaagtgctg gatgcgtcgg ccatggctca gtccaacgca aaggcaggaa
     1981 gaagttgcgc aggcgaatcg accacgcagt ccgcacccca gctgcgcgga tcctcgccat
     2041 cgaggtaccc ccagccggcc gcgatcgtgc gcatgccggc gcgatggcca gcctcgatgt
     2101 cgcgcaaatc gtcgccgaca tagatgcaac gcgccgcgga tacgccaaca atttcgcagg
     2161 cgcgcaggat cggtgccgga tcgggtttct ttagcggcag gcagtcgccc gtcaccagcg
     2221 ccgcgcaacg cgcatccagg cgcagcgctg cgagcaacgc acgcgccagc gtgccgggct
     2281 tgttggtgac gatgccccaa tgcgtaccgc gcatctccag gccatcgagt accgcaccca
     2341 tgccgggaaa caaagccgaa tgcgtggcga tggcctgcgc gtagagatcg aggaatcgtg
     2401 gcagcaggac ttcgacgcgc gcctcgtccg ccgcgggaaa cccgcgccgg atcatggcgc
     2461 gaccgccctt ggagaccacg gcgcctatcg attcggcgcc atccggcgac tcgccctctt
     2521 ccaggcgcag gcgtcgcatg gcctcgacca tgtcgggggc ggaatcgacc agtgtgccgt
     2581 cgaggtcgaa aaaaaccgct gcgggagtcg attgccgcat cacaccggct tccgcgtgca
     2641 ggcaagatag tttacttgtg tacttttcac gatcgcggcg cggcgcgtga acggattgta
     2701 ggcaagcccg ctcaggtctt caagttccag accgtgttcg cgcaacatgg tggccaattc
     2761 ggacggccgg atgaattgcg cgtagtgatg cgtgccgcgc ggcaacaggt tcagcgcgta
     2821 ctcggcgccg gcgatcgccg cgccgaatgc cagcggcgtg cggttgagcg tggacacgaa
     2881 caatcgcccg cccggcttga gcaaggtcgc acacgcctgg atgacgctgc cgggatcggg
     2941 cacatgctcg agcagttcca tgcacgtcac cgcatcgaac gcgcccggca tttgcgccgc
     3001 cagcgcctcc acggcgattt cgcggtattc gacccgcacg cccgattcgt gcagatgcag
     3061 gcgcgcgact tcgagcacgc gcgacgccag gtcgatgccg gtcacgtccg cgcccgcacg
     3121 cgcgagcgac tcggaaagaa tgccgccgcc gcagccgaca tcgagcacgc gtgcaccctt
     3181 gagcgcgacg cgttcggcaa tgtagttcag acgtgcggga ttgaggtcgt gcagcggccg
     3241 cgattcgccg ttgatgtccc accagcgcgc ggcgagcgca tcgaattttg ccgtttcggc
     3301 gacgctgaca ttggtcgcgt tcatctcagt tcgccgcgat gcgcgcgcgc cacgcctgcg
     3361 ttttcgcgcg caaggcatcg gtgtcgattg cgcacaatgc gctattttcc actttgcgca
     3421 caccggcgat ccacacgtcg ccgacctgat ggcggccggt cgcgtagacg atctgcgaaa
     3481 tcgcgttgta cagcggctgc gtttccagcg cgtccaggcg cacggcgaca aggtcggcct
     3541 gctt
//
LOCUS       DASRVO010000317         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_9686, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000317 DASRVO010000000
VERSION     DASRVO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9686"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1000
                     /locus_tag="VFF05_03915"
     CDS             <1..>1000
                     /locus_tag="VFF05_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW50982.1"
                     /translation="GDARTGTLTAQFILMQGRPHDAALTALDAARVAGDHTDVLGDLI
                     ETLLQVGESATAHDLLQRPVWQQAVDADTLLRYADLRKRFGEHAQALATFDRLVALRP
                     DDAMLRRHRGQQLEFLGRMDEAEAEYEACLARLPGYGRAAYSLVRLRKQTRDDHHLAM
                     IEAGQRQTRPSSAEHADFDFARYHVLEDLGETEGAWQALATANASMHALAAADAAREL
                     AGTQHFCELAATRPPRATSVRHEGPRPIFIIGLPRSGTTVLERMLANHSQVASTGELT
                     DFGRQLLRVGNTAAGWDADFFTRQLGLDFAEVGRGYLAQTRWRAGDKSFYIDKRPGN"
BASE COUNT          176 a          335 c          327 g          162 t
ORIGIN      
        1 ggggacgcac gcaccggcac gctgactgcc cagttcatcc tgatgcaggg ccgtccgcac
       61 gacgcggcat tgactgcact ggacgcggcg cgagtcgcgg gcgaccacac cgacgtgctc
      121 ggcgatttga tcgaaacgct gctgcaagtc ggcgaatccg cgaccgcgca cgatctgctg
      181 cagcgaccgg tgtggcagca agccgtggat gcggacacgc tgctgcgcta tgcagacctg
      241 cgcaaacggt tcggcgaaca cgcgcaggcg ttggccacat tcgaccggct cgtcgcgttg
      301 cgtcccgatg acgcaatgct gcgccgccat cgcggtcagc agctggaatt tctcggacgc
      361 atggacgaag ccgaggcgga atacgaggcc tgcctcgcgc gccttcccgg ctatggccgc
      421 gcggcgtatt cgctggtgcg cctgcgcaag caaacccgcg acgaccatca tctggcgatg
      481 atcgaggccg gccagcggca aacccgtccc agcagcgcag aacatgccga tttcgacttc
      541 gcccgctatc acgtactgga agatctgggc gaaaccgaag gcgcgtggca ggccctggct
      601 acggccaatg cgtcgatgca cgccctggct gcggcggacg cggcgcgcga gctggccggc
      661 acccaacatt tttgcgagct ggctgcgaca cgccctccgc gcgcgacaag cgttcgtcac
      721 gagggcccgc gcccgatttt catcatcggc ttgccgcgtt ccggcaccac cgtgcttgaa
      781 cgcatgctgg ccaatcactc gcaagtcgcc agtaccggcg agctgaccga tttcggacgg
      841 cagttgctgc gcgtcgggaa taccgccgcg gggtgggacg cggatttctt cacgcgtcaa
      901 ttagggctgg attttgccga ggtgggacgc ggttatctcg cgcaaacgcg ctggcgcgcc
      961 ggcgacaaat cgttctacat cgacaagcgg cccggcaact
//
LOCUS       DASRVO010000318         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_36143, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000318 DASRVO010000000
VERSION     DASRVO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36143"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VFF05_03920"
     CDS             complement(<1..903)
                     /locus_tag="VFF05_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW50983.1"
                     /translation="MSNFFIDRPIFAWVIAILIALGGAISLLNLGVESYPSIAPPQVV
                     VNAAYPGADAATVEKSVTQVIEQQLTGIDHLMYFSSSSSANGGVGVTLTFEPGTDPDI
                     AAVQTQNKVNLATPRLPAEVVKQGVTVTKSNPDFLMFFALKSKDGSLDSYRLNNLLSS
                     GILDQIARLPGVSNTFQVGAEYAMRIWLDPDKLHGYKLSAADALAAVRAQNVQVAAGS
                     IGAEPTVPGTGVTATVRAEGRFSTPDQFGNIILRANADGTTVRLKDVARIELGPASYG
                     QRTSYNGVPVAGAGVQLATGANALT"
     gene            complement(976..>1105)
                     /locus_tag="VFF05_03925"
     CDS             complement(976..>1105)
                     /locus_tag="VFF05_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HZW50984.1"
                     /translation="TSGLADGDKIIVSGVQHVQAGAPAKAAPWKPEAAPPAAKSAH"
BASE COUNT          176 a          334 c          382 g          213 t
ORIGIN      
        1 ggttagcgcg ttggcgccgg tggcgagctg cacaccggcg ccggcgaccg gcacgccgtt
       61 gtagctggtt cgctggccgt aactggccgg tcccagttcg atgcgcgcca cgtccttcag
      121 gcgcaccgtg gtgccgtcgg cgttcgcgcg caggatgatg ttgccgaatt gatccggcgt
      181 gctgaaacgc ccttccgcgc gcacggtcgc ggtgacgccg gtgccgggaa ccgtcggttc
      241 ggcgccgatg gaaccggccg cgacctgcac gttctgcgcg cgcacagccg ccagcgcatc
      301 ggccgccgac agtttgtagc cgtgcagctt gtccggatcg agccagatgc gcatcgcgta
      361 ttcggcgccg acctggaacg tgttgctcac gccgggcagg cgcgcgatct ggtcgaggat
      421 gccggacgaa agcagattgt tgagccggta ggaatccagg ctgccgtcct tcgacttgag
      481 cgcgaagaac atgaggaagt cggggttgga cttggttacc gtcacgccct gcttcactac
      541 ttcggccggc aggcgtggcg tcgccaggtt caccttgttc tgggtctgta ccgcggcgat
      601 gtcgggatcg gtgccgggct cgaaagtgag ggtgacgccg acgccgccgt tcgcgctgga
      661 gctcgaactg aagtacatca gatgatcgat gccggtaagc tgttgctcga tcacctgggt
      721 gacgcttttc tctaccgtgg ccgcgtccgc gcccggatag gcggcgttga ccacgacttg
      781 cggcggcgcg atcgacggat acgattccac gccgaggttg agcagggaaa tggcgccgcc
      841 gagcgcgatc aggatggcga tgacccacgc gaagatgggg cgatcgatga aaaaattcga
      901 catgggattt ccctttgcgt tcgtccctga tcgcgagaat caaaaagcag ccatgctggc
      961 cgtactttta aatggttagt gggcggactt ggcggctggc ggtgcggctt ctggcttcca
     1021 cggcgccgcc ttggctggcg ctcccgcttg cacatgctgc acgcccgaga cgatgatctt
     1081 gtcgccatcg gccagacccg aggtg
//
LOCUS       DASRVO010000319         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_243173, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000319 DASRVO010000000
VERSION     DASRVO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243173"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..701)
                     /gene="tolQ"
                     /locus_tag="VFF05_03930"
     CDS             complement(18..701)
                     /gene="tolQ"
                     /locus_tag="VFF05_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZW50985.1"
                     /translation="MNDGINVLQLALNASLFVKVVMAILIVFSFLSWVIIFRKMNVLN
                     RAMKGADDFEERFWSGADLAGLFRDVTANGDRVTGIESIFEAGFREFARLRQRRQMDP
                     RTILEGAQRGMRVALTREIDRLEQNLEFLANVGSISPYVGLLGTVAGIMVAFVGLANV
                     KEATIAMVAPGISEALIATAMGLIAAIPAVWAYNRYSNKVERIITRYDTFVEEFSSIL
                     QRQAHLDEA"
     gene            complement(694..>1015)
                     /locus_tag="VFF05_03935"
     CDS             complement(694..>1015)
                     /locus_tag="VFF05_03935"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZW50986.1"
                     /translation="ERARTEWLRACGLEQARVREEHGILFVVAEMRVKFLAPARLDDV
                     LHADITAFTRRSASMRFAQCILRPADGALLIEADVRAACIDAARYKPRRIPDSLLSEF
                     VPHE"
BASE COUNT          181 a          318 c          342 g          174 t
ORIGIN      
        1 gacgcgtcat gccggggtta ggcttcgtcg aggtgggctt gtctttgcag gatcgaggag
       61 aactcctcca cgaacgtgtc gtagcgcgtg atgatgcgct cgaccttgtt cgagtagcgg
      121 ttgtaggccc acaccgccgg aatcgccgcg atcaggccca tcgccgtggc gatcagcgct
      181 tcggagatgc ccggcgcgac catggcgatg gtcgcttcct tgacgttggc gaggccgacg
      241 aacgcgacca tgatgccagc cacggtgccg agcaggccga cgtaggggct gatcgagccg
      301 acattggcga ggaattccag gttctgctcg aggcggtcga tctcgcgcgt gagcgccacg
      361 cgcatgccgc gctgggcgcc ttccaggatc gtgcgtgggt ccatctggcg gcgctggcgc
      421 aggcgtgcga attcgcggaa tcccgcctcg aagatgcttt cgatgccggt cacgcggtcg
      481 ccgttcgcgg tgacgtcgcg gaacaatcca gccaaatccg cgccggacca gaagcgttcc
      541 tcgaaatcgt ccgcgccctt catcgcgcgg ttgagcacgt tcatcttgcg gaagatgatc
      601 acccacgaca ggaacgagaa cacgatcagg atcgccatca cgactttgac gaacaggctg
      661 gcgttgagcg ccagctgcag gacattgatg ccgtcattca tgcgggacga actccgaaag
      721 cagggaatcg ggaatgcggc gcggcttgta acgggccgcg tcgatgcagg cggcgcgcac
      781 gtcggcttcg atcagcagcg cgccgtccgc gggccggagt atacattgcg cgaagcgcat
      841 cgaggccgaa cgccgcgtga acgcggtgat gtccgcgtgc aacacgtcgt cgagtcgagc
      901 gggcgccagg aacttcaccc gcatctcggc gaccacgaac aagatgccgt gttcttcgcg
      961 cacgcgtgcc tgctccaggc cacacgcgcg cagccactcg gtgcgtgcgc gttcg
//
LOCUS       DASRVO010000320         1350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_321585, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000320 DASRVO010000000
VERSION     DASRVO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321585"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="ftsY"
                     /locus_tag="VFF05_03940"
     CDS             complement(<1..937)
                     /gene="ftsY"
                     /locus_tag="VFF05_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZW50987.1"
                     /translation="MLKFWKKQESESGNRKPESPSENPESVKKTWRERLAGSVLNRDI
                     RDLFARHPKLDESLLDDLETILIGADVGVAASTELIEALRARLAKHEFADAGQLLAAL
                     RERLVALLAPVARPLRIDPAHKPFVILMVGVNGVGKTTTIGKLARRLQNDGNEVLLAA
                     GDTFRAAAVEQLKTWGERNGVAVIAQSSGADSASVIFDALQAAKSRGTDVLIADTAGR
                     LHTQGGLMDELAKIRRVLAKLDAQAPHEALLVIDGTTGQNAINQVRQFKQAIGVTGLV
                     VTKLDGTAKGGVVFALAREFGLPIRYIGLGETLADL"
     gene            967..>1350
                     /gene="rsmD"
                     /locus_tag="VFF05_03945"
     CDS             967..>1350
                     /gene="rsmD"
                     /locus_tag="VFF05_03945"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZW50988.1"
                     /translation="MVSPRPASPSGKLRIIGGSLRGSRIAVPDAPGLRPTPERVRETL
                     FNWLAPVIHGARCLDLFAGTGALGIEALSRGAAQVDFVEADARLADLLRANLGRLRQD
                     ARVHAADAQRFLEQGGEHYDVVFLDP"
BASE COUNT          206 a          436 c          447 g          261 t
ORIGIN      
        1 gcaggtcggc gagcgtctcg cccagaccga tgtagcggat cggcaggccg aattcgcgcg
       61 ccagcgcaaa caccacgccg cccttggcag tgccgtcgag cttggtcacg accaatcccg
      121 tgacgccgat cgcctgcttg aactggcgca cctggttgat cgcgttctgg cctgtggtgc
      181 cgtcgatcac gagcagggct tcgtgtggtg cctgcgcgtc gagcttggcc aacacacgac
      241 gaatcttggc gagttcgtcc atcagtccgc cctgcgtgtg caggcgaccg gcggtatcgg
      301 cgatcagcac atccgtgccg cgggattttg ccgcttgcag cgcatcgaag atcacgctgg
      361 ccgaatccgc gccggatgat tgcgcgatca ccgccacgcc gttgcgctcg ccccaggtct
      421 tgagctgttc gaccgcggcg gcgcggaagg tatcgccggc ggcaagcagg acttcgttgc
      481 cgtcgttctg caatcgccgc gcgagcttgc cgatggtggt ggtcttgccg acgccgttca
      541 caccgaccat gagaatcacg aaaggcttgt gcgccggatc aatgcgcagc ggtcgcgcga
      601 ccggcgcgag cagggcgacg aggcgttcgc gcaacgccgc cagcagttgc cctgcatcgg
      661 cgaattcgtg tttggcgagg cgcgcgcgca aggcttcgat caactcggtg ctggcggcga
      721 cgccgacatc ggcgccgatg agaatcgttt cgagatcgtc gagtagggat tcgtcgagtt
      781 tgggatggcg cgcgaacagg tcgcggatat cgcgattgag cacgctgccg gccaagcgtt
      841 cgcgccaggt cttcttcacc gattcggggt tttcggaagg gctttccggc ttgcggtttc
      901 cgctttccga ttcctgtttc ttccagaact tgagcattgc gcatcgactt ccacgacaat
      961 ggcaccatgg tatcaccgcg acccgcgtca ccttccggaa aactgcgcat catcggcggc
     1021 tcgttgcgcg gttcgcgcat tgcggtgccg gacgcgccgg gcctgcgccc gacgccggag
     1081 cgcgtgcgcg aaacgctgtt caactggctg gcgccggtta tccatggcgc ccgctgcctg
     1141 gatcttttcg ccggcacggg tgcgctcggc atcgaagcgc tgtcgcgcgg cgccgcgcag
     1201 gtggatttcg tcgaggccga tgcacggctc gccgacctgc tgcgcgccaa cctgggccga
     1261 ctcaggcagg atgcgcgtgt acatgccgcc gatgcccagc gttttctgga acagggcggc
     1321 gaacattacg acgtcgtctt tctcgatccg
//
LOCUS       DASRVO010000321         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_164724, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000321 DASRVO010000000
VERSION     DASRVO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164724"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFF05_03950"
     CDS             complement(<1..328)
                     /locus_tag="VFF05_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZW50989.1"
                     /translation="MSLRFKLLLIALSTLALPLAGWLFVRQMEQLLRQGQEQTLIATA
                     NALAQSLDVLGVPLPPTGAALFVHREPGRMVVDGYGDDWNLLAPFAQNLGPASEPGKL
                     SLLLCDD"
     gene            complement(341..>1092)
                     /gene="pdsR"
                     /locus_tag="VFF05_03955"
     CDS             complement(341..>1092)
                     /gene="pdsR"
                     /locus_tag="VFF05_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HZW50990.1"
                     /translation="RLPRASAPRKLAVPPTSGIRMARHIAIVEDEPAIRANYVEALTR
                     IGYQVQGYGSRPDASRAFALRLPELVIIDVGLGDEPEGGFDLCRELRAKSPTLPIIFL
                     TARDSDFDVISGLRLGADDYLTKDVSLHHLAARISALFRRMDALGRPSAKETVVEHGP
                     LKLEAERMRITWNGAEIPLTVTEFWMVHTMVRFPGHVKNRDQLMREAQVVVDDATITS
                     HIKRIRKKFIAIDPAFDAIETVHGAGYRWKP"
BASE COUNT          185 a          332 c          381 g          194 t
ORIGIN      
        1 cgtcgtcgca cagcagcaag gacagcttgc cgggctcgga tgccggcccg agattttgcg
       61 caaacggtgc gagcaggttc cagtcgtcgc cgtagccgtc cacgaccatg cgccccggtt
      121 cgcggtgcac gaacagcgcg gcgcccgtgg gcggcagcgg cacgccgagc acatcgagac
      181 tttgtgccag cgcgttcgcc gtggcgatca aggtctgttc ctggccctgg cgcagcaact
      241 gctccatctg gcgcacgaac agccagcccg ccagcggcag ggccagcgtg gaaagcgcga
      301 tcaggaggag tttgaaacgc agcgacatgg tgatcgcgcg tcagggcttc cagcgatagc
      361 cggcgccgtg aacggtttca atcgcatcga acgccggatc gatggcgatg aatttcttgc
      421 ggatgcgctt gatgtgcgag gtgatggtcg catcgtcgac gacgacctgg gcttcacgca
      481 tgagctggtc gcgattcttt acatggccgg gaaagcgcac catggtgtgc accatccaga
      541 actcggtcac ggtcaacggt atttcggcac cattccaggt gatgcgcatg cgttctgcct
      601 cgagcttgag cgggccgtgt tcgaccacgg tttccttcgc gctcggtcgg cccagggcat
      661 ccatgcgccg gaacagtgcg ctgatgcgtg cggccagatg gtgcaggctc acatccttgg
      721 tcaggtagtc gtcggcgccc aggcgcaggc ccgagatgac gtcgaaatcc gaatcgcgcg
      781 cggtcaggaa aatgatcggc agcgtcggcg atttggcgcg cagttcgcgg cacagatcga
      841 agccgccttc gggctcgtcg ccgaggccga cgtcgatgat gacgagttcg ggcaggcgca
      901 gcgcgaacgc gcggctggca tccgggcgcg agccgtagcc ttgcacctgg tagccgatgc
      961 gcgtgagcgc ttcgacgtag ttcgcgcgga tggcgggttc gtcttcgacg atggcgatat
     1021 ggcgggccat gcggattcct gatgttggcg gcacagccag cttacggggc gcgctcgcgc
     1081 gcggcaatcg gc
//
LOCUS       DASRVO010000322         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_61726, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000322 DASRVO010000000
VERSION     DASRVO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61726"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFF05_03960"
     CDS             complement(<1..219)
                     /locus_tag="VFF05_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50991.1"
                     /translation="MTKFLRKTLTATAVVAALAAGSAAAADTKPAAHAAPAAAAAPAV
                     DKAAIDAKFKGDKKAEYSYMVGMDVGRGL"
     gene            377..2260
                     /locus_tag="VFF05_03965"
     CDS             377..2260
                     /locus_tag="VFF05_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW50992.1"
                     /translation="MIRLDSLTLRRGPQVLLENVDLALHAGWRVGITGRNGAGKSSLL
                     ALVAGELAPDAGHFERPANWSMAWVRQEVPALESTALDYVLDGDAELRAIERDLVAAE
                     DAHDGMRIAHLHERLHTIGGYAAPARAGALLHGLGFAAGDETRRVAEFSGGWRMRLNL
                     AQALMCRSDLLLLDEPTNHLDLDAVLWLQDWLARYPGTLLLISHDREFLDAVVTHVLA
                     IAERRADLFTGDLTGYERKRAEQLTQQSAAFEKQQRERAHLQHFVDRFRAKATKARQA
                     QSRIKALERMVEIAPVRAASTIQFEFREPARLPAPLLKLDHAAAGYGERHVLDDITLI
                     LAPGDRVALLGANGAGKSTLVKLLAGQIAPLAGERIAAKDLAIGYFAQHQLEQLDPSA
                     SAVAHLLRLDPALGEKAARDFLGGFGFAGERALEPVAPFSGGEKARLCLALTVFQRPN
                     LLLLDEPTNHLDLDMREALTEALNDFAGAVVLVAHDRALIRACCDTLLHVGAGRVTPY
                     DGDLDDYARQVLRAANTNATTPEKPASGNKRRDARRANADERARSTTLQQEALRLEKR
                     IAQVESDKRQTASALADPALYDGSQNAQIQRLTRQAAVLAAELGKLEEQWLSAHEALE
                     RGI"
     gene            2439..2798
                     /locus_tag="VFF05_03970"
     CDS             2439..2798
                     /locus_tag="VFF05_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW50993.1"
                     /translation="MQALLIRAAIAVATGLMLTAAMFIDHGGSAAHAVTPAATMAKSA
                     RSIMPEPVTLPTIHVHPTAADLAAAAMPKRNPARFVIEVALPALPASAASTLPRGLNV
                     DMPYYSFGKVLPRIGKE"
     gene            2801..3172
                     /locus_tag="VFF05_03975"
     CDS             2801..3172
                     /locus_tag="VFF05_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZW50994.1"
                     /translation="MSIIWNDNTPIYRQLRDRVVAMILDGALKPGDALPSVRQVAADF
                     QLNPITVSKGYQELVDEQLVEKRRGLGMYVTEGARATLMKSEREHFLREEWPALFARI
                     QRMNLDLATLIKNAATTGEKS"
     gene            3169..>3524
                     /locus_tag="VFF05_03980"
     CDS             3169..>3524
                     /locus_tag="VFF05_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW50995.1"
                     /translation="MTAVVQAKGLSKRYGNTVALDNVNLDIQPGRIVGLVGPNGAGKT
                     TALKAILGLAEFDGQLSVLGLDPRTHRTELMQQVCFIADVAVLPRWLRVSDAVDFVGG
                     VHPRFSREKCEKLLART"
BASE COUNT          640 a         1188 c         1099 g          597 t
ORIGIN      
        1 caggccccgg cccacgtcca tgccgaccat gtagctgtat tcggccttct tgtcgccctt
       61 gaacttggcg tcgatggcgg ccttgtcgac cgcgggcgcc gcagcagccg cgggggccgc
      121 atgcgcggcc ggcttggtgt cggcagcggc ggcggaaccg gccgcgagcg cggcgacaac
      181 cgccgtggcg gtcagggttt tgcgcaaaaa cttcgtcatg tgtgctcctg gatgaatggt
      241 attgccgatc gcgcggcatg cctacgaatt ttggcgggcc gcgtattgtg cggttccgga
      301 ccgccgaagg caaacgggtt cgggttggac tgcatccgcc cggcaaggtt cccgtcgcgt
      361 acgttaaaat tcgccgatga tccgtctcga tagcctcacc ctgcgccgcg ggccgcaggt
      421 tctgctcgaa aatgtcgacc ttgccctgca cgccggctgg cgcgtgggca tcaccggccg
      481 taacggcgcg ggcaaatcca gcctgctcgc ccttgtcgcc ggcgaattgg cgccggatgc
      541 cgggcatttc gagcgtccgg ccaactggtc gatggcctgg gtgcgccagg aggttccggc
      601 gctggaatcc accgcgctcg actatgtgct cgacggcgac gccgaactgc gcgccatcga
      661 acgcgacctg gtcgccgccg aagacgccca cgacggcatg cgcatcgcgc acctgcacga
      721 gcgcctgcac acgatcggcg gctatgcggc gcccgcgcgc gccggcgccc tgctgcacgg
      781 cctgggcttc gccgccggcg acgaaacccg ccgcgtcgcg gaattctccg gcggctggcg
      841 catgcgtttg aatctggcgc aggcgctgat gtgtcgatcg gacctgctcc tgctcgacga
      901 gccgaccaat cacctggacc tggatgccgt gctctggctg caggactggc tggcgcgcta
      961 tccgggtacc ttgctgctga tctcgcacga ccgcgagttt ctcgatgccg tcgtcaccca
     1021 tgtgctggcc atcgccgagc gccgtgcgga tttgttcacc ggcgacctga ccggctacga
     1081 gcgcaagcgt gccgagcaac tgacgcagca aagcgccgcc ttcgaaaaac agcaacgcga
     1141 acgcgcgcac ctgcagcact tcgtcgaccg ttttcgcgcc aaggcgacca aggcgcggca
     1201 ggcacaaagc cgcatcaagg cattggagcg catggtggaa attgcgccgg tgcgtgccgc
     1261 ctcgacgatc cagttcgaat tccgtgaacc cgcacgcctg cctgcaccgc tgctcaagct
     1321 cgaccatgcc gccgccggtt acggcgagcg ccacgtgctc gacgacatca ccttgattct
     1381 cgcccccggc gaccgcgtcg ccctgctcgg cgccaacggc gcgggcaaat ccaccctggt
     1441 gaaattgctg gccgggcaaa tcgcaccact ggccggcgaa cgcattgccg caaaagacct
     1501 cgccatcggt tatttcgcac agcatcagct cgaacagctc gatccttccg cgagcgccgt
     1561 ggcgcacttg ctgcgcctgg atccagcgct gggcgaaaag gccgcgcgtg atttcctcgg
     1621 cggattcggc tttgccggcg agcgcgcgct agaaccggtg gcgccgtttt ccggtggcga
     1681 gaaggcgcgg ctgtgcctag cgctgacggt tttccagcgg cctaacctgt tgctgctgga
     1741 cgaaccgacc aaccacctcg acctcgacat gcgcgaagca ttgaccgagg cgctgaacga
     1801 ttttgccggc gccgtggtgc tggttgcgca cgaccgcgcc ctgatccgcg cctgctgcga
     1861 cacgctgctg cacgtcggcg cgggacgcgt cacgccctac gacggcgatc tggacgacta
     1921 tgcacgccag gtgttgcgcg ccgcgaacac gaatgcgacg acgcccgaga aacccgcatc
     1981 cggcaacaaa cgtcgcgacg cacgccgcgc caatgcggac gaacgtgcgc gctcgacgac
     2041 tctgcagcag gaggcgctgc gcctcgaaaa acgcatcgcc caggtcgaat ccgacaaacg
     2101 ccaaaccgct tccgcgctgg ccgacccggc cctctacgac ggcagccaga acgcgcaaat
     2161 acaaagactg acgcggcaag cggcggtgct ggccgccgag ctgggcaaac tcgaagagca
     2221 atggctgtcc gcacatgaag cgctggagcg cggaatctga ccgcccgccg gaatgcacag
     2281 catattgctg tttttaaacg tttttgtctg aatttccggc aactatagtc acccgcgatg
     2341 atgttttttg tttgtggtgc ttgacaagtc cagcctaggt gttatactta cctacaacac
     2401 tagtacgaca aatcactgga cgggagacaa ccatgtactt gcaagcactg ctgatccgcg
     2461 ccgccatagc ggtcgccacc ggcttgatgc tgaccgccgc catgttcatc gaccatggcg
     2521 gatcggccgc ccacgccgta accccagccg ccacgatggc gaaatccgcc cgcagcatca
     2581 tgccggaacc ggttacgctg ccgacgatcc acgtgcatcc gacagcggct gatcttgccg
     2641 ccgctgccat gccgaagcgg aatccggccc gcttcgtcat cgaagtggcg ctgcccgcct
     2701 tgcccgcctc cgctgcctcg acattgccgc gcgggctgaa cgtcgacatg ccttactatt
     2761 cttttggcaa ggtattgccg cggatcggca aggaatgagc atgagtatca tctggaacga
     2821 caacacgccg atctacaggc aactgcgcga ccgcgtggtg gcaatgatcc tggacggcgc
     2881 gctcaagccg ggcgatgccc tgccttcggt gcgccaggtg gcggccgatt tccaactcaa
     2941 tccgatcacc gtgtcgaaag ggtaccagga actcgtcgac gaacaactcg tggaaaaacg
     3001 cagggggctg ggcatgtacg tgacagaagg agcgcgcgcg acgctgatga aatccgaacg
     3061 cgaacacttt ctgcgcgagg aatggccggc gctgttcgcg cgcatccagc gcatgaacct
     3121 ggacctggcc accttgatca agaacgccgc aactaccgga gaaaaatcat gactgccgtc
     3181 gtgcaggcaa aaggactgtc caagcgctac ggcaacacgg tcgcgctgga caacgtgaac
     3241 ctggacatcc agcccggccg catcgtcggc ctggtcgggc cgaacggcgc cggcaagacc
     3301 acggcgctca aggccatcct cgggctggcc gaattcgacg gccagttgtc ggtgctcggc
     3361 ctggatccgc gcacgcatcg caccgagctc atgcaacagg tgtgcttcat cgccgacgtg
     3421 gccgtgctgc cgcgctggct gcgcgtcagc gacgcggtgg atttcgtcgg tggcgtgcat
     3481 ccgcgctttt cgcgcgagaa atgcgaaaag ctgctggcac gcac
//
LOCUS       DASRVO010000323         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_373035, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000323 DASRVO010000000
VERSION     DASRVO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373035"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFF05_03985"
     CDS             complement(<1..378)
                     /locus_tag="VFF05_03985"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HZW50996.1"
                     /translation="MRLPALFFGLLAAASSRAAVVYDFTPVTQQIDTLLQTYPDIGGA
                     SLIVIRNGAVVYENYFGSYSPATRVLIASASKWLSALTIERLVEKNQMSWGDTVGQYI
                     PNAPADKFGITLGQLFSHTSGLTQ"
     gene            complement(442..1617)
                     /locus_tag="VFF05_03990"
     CDS             complement(442..1617)
                     /locus_tag="VFF05_03990"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HZW50997.1"
                     /translation="MSRPDNPTIRLKIERRSNHPWIFQKMVEKPEPKPKPGSIVDIVD
                     REGSFAGRGFYNGHSRIALRVLTSNPDEAVDEDFFARKISDAVALRRDLLKLDAVTDA
                     YRLIHSEGDGLSGLVVDRFANTLVVEYFSAGFFKQRDLIKRVLLQHFPDAEIYAFAED
                     HVQKQESFDLAHPPAPKPTVIHEHGVQFHAAPGSKHKTGFFADQRDNRLFLSEFCAGK
                     RVLDLCCNSGGFGIYAKARGGAEAVVGIDLDEEILGIAEQNARLNKARVKFIQADIFA
                     WLRDTASNPAKKFDVVILDPAKMTRDREQVIPALKKYLDMNKLALGAVKPGGMFLTCS
                     CTGLVSEEQFLDMLRRAAFYANRTVQVIKVSGAGPDHPWQAHVPESRYLKAAFCRVE"
     gene            complement(1643..>2663)
                     /locus_tag="VFF05_03995"
     CDS             complement(1643..>2663)
                     /locus_tag="VFF05_03995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZW50998.1"
                     /translation="VPDWLSIVGANSLLSLAFAQQVHAVRLFGGLPLGRVAVYAPAVA
                     VLLDEIVFTYAVPSMRLRLLTATPLFCLQMIQGGLSLVSSAAVSRRSGRLTAAAFFAL
                     AAVLVARVVYESLHTEVLRSAFWDSPMQTIVFSFASLFVVVATLGFVLMCSDQLYREL
                     ERQAMLDPLTGISNRRTLDLLAMQALASAQRHTRPLALLLIDADHFKRINDECGHAAG
                     DDALRLLAATLRRGLRNEDLLGRLGGEEFVAVLPESDTEDALASAERLRRAVEELDFT
                     VPDRPVPLRISVGVATLRDGDDLATLLRRADAAMYAAKRAGRNCVRGPADVADPSAAG
                     AIRMG"
BASE COUNT          496 a          842 c          874 g          451 t
ORIGIN      
        1 ctgggtcagc cccgaggtgt ggctgaacag ctgaccaagc gtgatgccga acttgtcggc
       61 cggtgcgttc gggatgtact ggccgaccgt gtcgccccaa ctcatctggt ttttctcgac
      121 caggcgttcg atagtcagtg ccgacagcca cttgctcgcc gaggcgatga gaacgcgcgt
      181 cgcgggcgaa taactgccga aatagttttc gtagacgacc gcgccattgc ggatgacgat
      241 cagcgaggcg ccgccgatgt ccggataggt ttgcagcagt gtgtcgatct gctgcgtaac
      301 cggcgtgaaa tcgtagacga ccgccgcgcg cgacgatgcc gcggcgagca atccgaaaaa
      361 cagggcgggt aggcgcacga acgtttcctt gtcgcgtcgg gagccatcct gaacgcagca
      421 gtcccaggat ggttgacgcg cctactccac ccgacagaac gctgctttca ggtaacgcga
      481 ctcgggcacg tgcgcctgcc aggggtggtc ggggcccgcg ccggacacct tgatcacctg
      541 caccgtgcga ttggcgtaga acgccgcacg ccgcagcatg tcgaggaatt gttcctcgct
      601 gaccagaccc gtgcacgagc aggtcaggaa catgccgccg ggcttgaccg cgccgagcgc
      661 gagcttgttc atgtccaggt atttcttcag cgccgggatc acctgttcgc ggtcgcgcgt
      721 catcttggcc gggtcgagaa tcacgacgtc gaatttcttc gccggattgc tcgccgtgtc
      781 gcgcagccag gcgaaaatgt cggcctggat gaatttcacc cgcgccttgt tgagccgtgc
      841 gttctgctcc gcgatcccga ggatctcctc atccaggtcg atgccgacga ccgcttccgc
      901 gccgccgcgc gccttggcat agatgccgaa gccgccggag ttgcagcaca ggtcgagcac
      961 gcgtttgccg gcgcagaatt cggacaggaa caggcggttg tcgcgctggt cggcgaagaa
     1021 tccggtcttg tgcttgctgc ccggcgcggc atggaactgc acaccgtgct cgtggatgac
     1081 cgtgggcttc ggcgccggcg gatgcgcgag gtcgaaactt tcctgcttct gcacgtggtc
     1141 ttcggcgaac gcgtagattt ccgcatcggg aaaatgctgc agcaacacgc gcttgatcag
     1201 gtcgcgctgc ttgaagaatc ccgccgaaaa atattccacg accagtgtgt tcgcgaaacg
     1261 atccacgaca aggcccgaca atccatcgcc ctcggagtgg atcaaacgat acgcatcggt
     1321 caccgcgtcg agcttgagca ggtcgcggcg caaggcgacg gcatccgaaa tcttgcgcgc
     1381 aaagaagtcc tcgtcgacgg cctcgtccgg attcgacgtg agcacgcgca gggcgatgcg
     1441 cgaatggccg ttgtagaaac cgcgcccggc gaaggagccc tcgcggtcga cgatgtcgac
     1501 gatgctaccg ggcttgggtt tcggctcggg tttttccacc atcttctgga aaatccaggg
     1561 atggttggag cggcgctcga tcttcaggcg gatggtcggg ttatcaggac ggctcatccg
     1621 tgcatgatac cggagcgggc gttcagccca tgcgtatcgc gccggccgcc gaagggtcgg
     1681 cgacatccgc gggcccgcgc acgcaattgc gcccagcgcg cttggccgcg tacatggccg
     1741 cgtcggcgcg gcgcaacagg gtcgctaagt cgtctccgtc gcgcagcgtg gcgacgccaa
     1801 cggagatgcg cagcggcacc gggcggtcgg gcactgtgaa gtcgagttcc tccacggcac
     1861 ggcgcaggcg ttcggcgctg gcaagcgcat cctcggtgtc gctttccggc agcacggcga
     1921 cgaattcctc accgcccagg cggcccagca ggtcctcgtt gcgcaatccg cgcctcagtg
     1981 tcgccgccag cagccgcaag gcatcgtctc ctgcggcatg gccgcattcg tcgttgatgc
     2041 gcttgaaatg atcggcatcg atcagcagca acgccagcgg acgggtgtgg cgttgcgccg
     2101 aagcgagcgc ctgcatcgcc agcaaatcca gcgtgcggcg gttgctgatg ccggtgagcg
     2161 gatcgagcat cgcctggcgt tcgagctccc ggtacagttg gtcgctgcac atgaggacga
     2221 agcccagcgt ggcgacaacc acgaacaacg aggcgaacga gaacacgatc gtctgcatcg
     2281 gcgagtccca gaacgccgag cgcaggacct ctgtgtgcag gctttcgtag acaacgcgcg
     2341 cgaccagtac tgcggccaaa gcgaaaaatg ccgccgcggt cagccggccg ctgcgcctgc
     2401 tcacggcggc gctggaaacg agcgacaggc cgccctggat catctgcagg cagaacaacg
     2461 gcgttgccgt cagcaggcgc agacgcatgc ttggcacggc gtaggtgaaa acgatttcgt
     2521 cgagcagcac cgcaacggcg ggagcataga ccgccacgcg cccgagcggc aggccgccga
     2581 acaggcgaac cgcatggacc tgctgggcga acgccagact cagcaaggaa ttcgcgccaa
     2641 cgatcgacag ccagtccgga acc
//
LOCUS       DASRVO010000324         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_164822, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000324 DASRVO010000000
VERSION     DASRVO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164822"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VFF05_04000"
     CDS             complement(<1..256)
                     /locus_tag="VFF05_04000"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZW50999.1"
                     /translation="MLIPRPETELLVELALQRIPQSAKVDIADLGTGSGAVALAIAQS
                     CPQARVLATDASAAALGVARGNAQRLGLGNVEFVQGDWCAA"
     gene            complement(269..508)
                     /locus_tag="VFF05_04005"
     CDS             complement(269..508)
                     /locus_tag="VFF05_04005"
                     /inference="COORDINATES: protein motif:HMM:NF037644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51000.1"
                     /translation="MRDLLADAQAALSGDDARGEAELLLAHVLGVDRAWLIAHRDDAV
                     ARALAGQVRCCVSRRVAGEPVAYIVGHRGFHAIDL"
     gene            complement(520..891)
                     /locus_tag="VFF05_04010"
     CDS             complement(520..891)
                     /locus_tag="VFF05_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51001.1"
                     /translation="MFNLSQIGLVLLTLVALASLISAVPKGLLGSPDMHVTGNGSTAQ
                     ALQWFADRSTDALPQASAVSVPLWVYKTLMLLWALWIANALIGWLRDAFAAWTRDGYW
                     RNSSKPAVAEDAAKEKPADAL"
     gene            complement(879..>3769)
                     /locus_tag="VFF05_04015"
     CDS             complement(879..>3769)
                     /locus_tag="VFF05_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51002.1"
                     /translation="HLRVTAASSAVQVDPRQSEVPGEWQNLPAFALGEGAKLSIEQRS
                     RGIAHDAANQLTLQREAWLDFAGGGWYARDNVRGTMVNGWRLDAAAPYALEQARAQNA
                     NSGSGEDLLVTRGAKPDSSGVEWRTPNVDLSANLRIAAPAAMSVAGWQQSFDNVQERL
                     HLPYGYKLLAAPGADSASGSWIAGWTLLDVFVCAILVLLAWRLLGLVGAAAAVAYLVL
                     GYQESDSPLWSLIAVIALALIARALPPGKLARATARLRAAALLILVLIALPFFATQVR
                     DALYPQLEAGGYAASPVREAPAAGQVEMQAKVAARRNDMAAMRSAPTPMSIQTPPPPP
                     ASAAALESIVVTGSRIPRSDVTDHYSQTTVVQTGAGMPSWNLGSVAVLSWSGPVLATQ
                     SVHLLIAPPWLVRPLRIALVALLAFLILRLFRGAVPDFKIPRRAALGAGLLVLGGLAL
                     APSAQAQGYPPQELLQQLRARLLEAPKCAPTCASVAQAQVAADGDNVSVVLEAHAGER
                     IALPLPNAGDSATLKSVQVDGVATDAVARGSDDTGWLELPRGVHRVQLAYAAAGDKVT
                     FAFSLKPARVRFQGKGWNVSGLADDRLLANSLTLARVRADAGAKPVFGVQQFPPYVQV
                     TRSINLGLEWSVSTSVQRLAPKTGGFTVSVPLLKGEHVASPGFKDVDGKVEAAIADGA
                     DATSWNSNLDKGGQLTLTAPALGDRAEVWRVVVSPTWHVEFSGVPGVDVSANDDANDY
                     RNFEFHPLPGETLTLTAIRPEALAGAQRAIDAASLRSAAGQHAATHTLDLTLRASQGG
                     DQVVTLPVDAQVLGVIRDNTPLNLRAQNGKLTLPLTPGIHRFEIRFRTDAALGLLAHT
                     PAVALGLPAANVNLALNLPENRWLLATWGPQAGPAVLYWGELIVMVLVAFALSRSRRT
                     RLKFRDWLLLGLGFSTFSWSALIVVVVWLFAFDCAAARRNRATNRCST"
BASE COUNT          643 a         1325 c         1241 g          560 t
ORIGIN      
        1 gcgcagcgca ccagtcgccc tgcacgaatt ccacgttgcc gagtccaagc cgctgcgcgt
       61 tgccgcgagc gacgccgagc gcggccgcac tcgcatccgt ggccagcaca cgcgcctgcg
      121 gacacgactg tgcgatggcc agcgcgaccg cgccggagcc ggtgccgaga tcggcgatgt
      181 ccacttttgc gctttgtgga atacgctgca aggcgagctc gaccaacagt tcggtttccg
      241 gccgtgggat cagcacgtcc ggcgtgagct acaagtcgat ggcgtggaat ccgcgatggc
      301 cgacgatgta cgcaaccggc tcgcccgcca cgcggcgcga gacgcagcag cgcacctgcc
      361 cggccaacgc gcgcgcgacc gcgtcgtcgc gatgcgcaat cagccaggcg cggtcgacgc
      421 ccagcacgtg cgcgagcaac agttccgctt cgccgcgtgc atcgtcgccg gacaatgcgg
      481 cctgcgcatc ggcaagcagg tcgcgcacgc gatattccgt cacaaagcgt ccgccggttt
      541 ttccttcgcg gcgtcttcgg caacggcggg cttcgacgaa ttgcgccaat aaccgtcgcg
      601 ggtccatgcg gcgaaggcat cgcgcagcca gccgatgagc gcgttcgcga tccacagcgc
      661 ccagagcagc atcagcgtct tgtagaccca tagcggcacg ctgaccgcgc tcgcctgcgg
      721 cagcgcatcg gtgctgcggt cggcgaacca ttgcagcgcc tgcgccgtgg atccgtttcc
      781 ggtcacgtgc atgtccggcg agccgagcag gcccttcggc acggccgaaa tcaggctggc
      841 cagcgcaacc agggtcagca acaccaggcc gatctggctc aggttgaaca acgattcgtt
      901 gcgcggttgc gcctcgcggc cgcgcagtcg aaggcgaaca gccacaccac gaccacgatc
      961 agcgcgctcc acgagaacgt cgagaagcca agaccgagca gcagccagtc gcggaatttc
     1021 aggcgcgtgc gccggctgcg cgagagcgcg aacgcgacca ggaccatcac gatcaactcg
     1081 ccccagtaca gcacggccgg gccggcctgc ggaccccagg tcgcaagcag ccagcggttt
     1141 tcgggcagat tcaacgccag attcacgttc gccgcgggca gcccgagcgc caccgccggc
     1201 gtgtgcgcca gcaggccgag cgccgcatcg gtacggaagc ggatctcgaa acgatggatg
     1261 cccggcgtca acggcaacgt gagcttgccg ttctgcgcgc gcagattgag cggagtgttg
     1321 tcgcgtatga cgccaagcac ctgtgcatcg acgggcaagg taacgacctg atcgccgccc
     1381 tgactcgcgc gcagcgtcag gtcgagcgta tgcgttgccg cgtgctgacc ggcagcgctg
     1441 cgcaagctcg cggcgtcgat cgcccgttgc gcgccggcca atgcctccgg ccgaatcgcg
     1501 gtcagtgtca gcgtttcgcc gggcaacgga tggaattcga agttgcggta gtcgttggca
     1561 tcgtcgttcg cggaaacatc cacacccggc acgccgctga attccacatg ccaggtcgga
     1621 ctgaccacga cgcgccagac ttccgcgcga tcgcccagcg ctggcgcggt cagggtcaac
     1681 tgcccgccct tgtcgagatt gctgttccag gatgtcgcgt cggcgccgtc agcaatggcg
     1741 gcttctacct tgccgtcgac gtccttgaag ccgggcgagg cgacgtgctc gcccttgagc
     1801 aggggcacgg agacggtaaa accaccggtc ttcggcgcga gccgctgcac gcttgtcgac
     1861 accgaccatt ccaggcccag attgatgctg cgcgtgacct gcacgtaggg cgggaactgc
     1921 tgcacgccga acaccggctt cgcgcccgcg tccgcgcgca cgcgcgcgag cgtgaggctg
     1981 ttcgcaagca ggcgatcgtc ggccagcccg gagacgttcc agcccttgcc ctggaaccgc
     2041 acccgcgccg gcttcagcga aaacgcgaac gtgaccttgt cgcctgccgc ggcgtaggcg
     2101 agctgcacgc gatgcacgcc acgcggcaat tccagccagc cggtgtcgtc ggaaccgcgc
     2161 gcgacggcat ccgtcgccac tccgtccact tgtacacttt tcaatgtagc gctgtcgccg
     2221 gcgttcggca gcggcaaggc gatgcgctcg ccggcatgcg cttccagcac gacgctgacg
     2281 ttgtcgccat ccgccgccac ctgcgcctgc gccacgctgg cgcaggtcgg cgcgcatttc
     2341 ggcgcttcga gcaggcgcgc gcgcagttgt tgcagcaact cctgcggcgg ataaccctga
     2401 gcttgcgcag acggggccag tgctagtcca ccgagcacca gcaagcctgc accgagcgct
     2461 gcgcggcgcg gaatcttgaa atccggcacg gcgccgcgaa acagacgcag gatcaggaac
     2521 gcgagcagcg ccaccagagc gatgcgcagc ggccgcacca gccacggcgg cgcgatcagc
     2581 agatgcacgc tctgcgttgc gagcaccggg ccgctccagc tcagcacggc cacgctgccc
     2641 agattccacg acggcatgcc ggcacctgtc tgcaccacgg tggtttggct gtagtggtcg
     2701 gtcacatcgg aacggggaat tcgcgaaccg gtcacgacta tggattcaag cgccgcagct
     2761 gatgcaggcg gtggcggtgg tgtctgaatc gacatcggcg ttggggccga cctcattgcg
     2821 gccatgtcat tgcgtcgcgc cgctaccttc gcctgcatct ccacctgccc cgccgccggt
     2881 gcctctcgca cgggactggc cgcatagccg cccgcttcaa gttgcggata caacgcatcg
     2941 cgtacctggg tggcgaaaaa gggcaacgca atcagcacga ggatgagcaa ggccgccgcg
     3001 cgcaggcgcg ccgtcgcgcg cgcgagcttg ccgggtggca gcgcccgcgc gatcaaggcc
     3061 agcgcgatca ccgcgatcag gctccacagc ggtgaatcgg attcctggta gccgagcacc
     3121 aggtacgcga ccgcggccgc cgcgccgacc agcccaagca gtcgccaggc gagcaggacg
     3181 aggatcgcgc acacgaacac gtcgagcagg gtccagcccg cgatccagct tccactggcg
     3241 ctgtccgcac cgggcgcggc gagcaatttg tagccgtagg gaagatgcag gcgttcctgc
     3301 acgttgtcga aagactgctg ccagccggcg acggacatgg ccgcgggcgc ggcgatgcgc
     3361 aggttcgccg acaggtccac gttcggcgtg cgccattcga cgccgctcga atccggcttg
     3421 gcgccgcgcg tgaccagcaa gtcctcgccg ctgccgctat tcgcgttttg cgcacgcgcc
     3481 tgctccagcg cgtagggcgc ggcggcatcc agacgccagc cgttgaccat ggtgccgcgc
     3541 acgttgtcgc gcgcatacca gccgccgccg gcgaaatcca gccaggcctc gcgttgcaag
     3601 gtcagctggt tcgccgcatc gtgcgcgatg ccgcgcgagc gttgctcgat cgaaagcttc
     3661 gcaccctcgc cgagcgcgaa cgcgggcagg ttctgccact ctcccggcac ttcggattgg
     3721 cgcggatcga cctgcactgc gctgctcgcc gcggtcacgc gcaaatgcg
//
LOCUS       DASRVO010000325         5314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_192360, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000325 DASRVO010000000
VERSION     DASRVO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192360"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="kdsA"
                     /locus_tag="VFF05_04020"
     CDS             <1..738
                     /gene="kdsA"
                     /locus_tag="VFF05_04020"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HZW51003.1"
                     /translation="LQMDVAGRLKDITGELGIPFIFKSSYDKANRSSGKSFRGLGMEK
                     GLEILDKVRRSIGVPVLTDVHAEDEISEVALVVDVLQTPAFLCRQTDFIHAVASAGKP
                     VNLKKGQFLSPWEMKHVAAKAKAVGNEQIMVCERGASFGYNNLVSDMRSLAVMRDTGC
                     PVVFDATHSVQLPGGADGKSGGQREFVPVLARAAVAVGIAGVFMETHPDPDKALSDGP
                     NAWPLGKMAALLETLVALDRVAKSQPL"
     gene            749..2047
                     /gene="eno"
                     /locus_tag="VFF05_04025"
     CDS             749..2047
                     /gene="eno"
                     /locus_tag="VFF05_04025"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZW51004.1"
                     /translation="MSQSITKIHAREILDSRGNPTLEAEVTLADGSFGRAAVPSGAST
                     GSREAVELRDGDKSRYLGKGVKNAVANVNGPIAQALKGLDAADQKGLDAKLIALDGTP
                     NKGKLGANALLGVSLANAHAMAASKKIPLWKYLAGSHAPSLPVPMMNIVNGGAHADNN
                     VDMQEFMILPVGLPSFAEALRAGVEIFHALKSVLKAKGLNTAVGDEGGFAPDLKSNEH
                     AIEVILEAIAKAGYKAGKDIYLGLDAASSEFFDNGMYDLAGEGRKMSSAELIEFYAGW
                     CAKYPIITIEDGVAEQDRDGWKALTAKLGKKIQLVGDDNFVTNPAIFRAGIADGIANA
                     ILIKVNQIGTLSETLETIAMADKANYAAVVSHRSGETEDTTIADIAVATTATQIKTGS
                     LCRSDRVAKYNQLLRIEEALGASAKYAGRAAFPNVPGLIG"
     gene            2058..2369
                     /gene="ftsB"
                     /locus_tag="VFF05_04030"
     CDS             2058..2369
                     /gene="ftsB"
                     /locus_tag="VFF05_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HZW51005.1"
                     /translation="MLRYIALILLILLIALQIKFWTGEGGMRDVWRLQERIAEQTAEN
                     RKLKQRNEALSAEVQDLKSGKGAIEERARSELGLIKPGEVFYQVVEPAPANPKRDNGN
                     H"
     gene            2356..3054
                     /gene="ispD"
                     /locus_tag="VFF05_04035"
     CDS             2356..3054
                     /gene="ispD"
                     /locus_tag="VFF05_04035"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZW51006.1"
                     /translation="MATTDALWCVVPAAGRGARVGGEVPKQYLPIAGKPMLLHTLERL
                     AAHPRVAGLMVVLEAGDVRWPKWLTFNDKPVSTTIGGAARADSVLAGLRALPEQVDKS
                     HFVLVHDAARPCVRVADVSHLLELGIPAGGALLAAPVRDTLKRADAQGRVVATEPREA
                     RWRAMTPQLFRLGELMQALESARDAGIAVTDEAMAMERVGHSPLLVEGSEDNIKVTTA
                     ADFALAQFILSRTD"
     gene            3054..3527
                     /gene="ispF"
                     /locus_tag="VFF05_04040"
     CDS             3054..3527
                     /gene="ispF"
                     /locus_tag="VFF05_04040"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZW51007.1"
                     /translation="MRIGQGIDIHAFGSGDHVVVGGVRIAHTRGVVAHSDGDVAIHAL
                     CDAILGALALGDIGQHFPPTDAHWRGADSRVFLRHCAGLMRERGFALGNADISIVCEA
                     PKIAPHAVAMRANLAVDLACDVGQVSVKATTSEKLGFTGRGEGIAALAVVLLTAA"
     gene            3529..4545
                     /gene="truD"
                     /locus_tag="VFF05_04045"
     CDS             3529..4545
                     /gene="truD"
                     /locus_tag="VFF05_04045"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HZW51008.1"
                     /translation="MDLPCAHGGATLRGILRATPEDFFVDEELGFAPDAAGEHVFVRV
                     EKRGANTDFVARELARFAGVRPDSVSYAGMKDRHAVTRQTFSIHLPGKADPDWASLAH
                     AEFRVLEAARHSRKLHRGALKGNRFRIVLREVEGDRDAAERIVESVRTQGVPNYFGEQ
                     RFGREASNVEKARAMFQGRRVQRHERSLFLSAARSQMFNAVLAERVEKGNWNRPLDGD
                     VFMLAGTHSIFGPEPLTPELVARLERGDIAPTGPMWGAGDLRSRDAVAELENSVAARY
                     PDLAAGLSVAGLRQERRSLVLRPRELELGWLQDSAAELSFYLSSGAYATVLLREIFDY
                     RNPA"
     gene            complement(4542..5090)
                     /locus_tag="VFF05_04050"
     CDS             complement(4542..5090)
                     /locus_tag="VFF05_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZW51009.1"
                     /translation="MRKSKSVTISEDDLRLFADAVGPVRRLHARERADAAPKPAPEPT
                     QSDRDEASVADELLDSAIDPAAIEIGEELSYLKDGHSPRLLRQLKRGHFSIADEIDLH
                     QMTAAVAREAVKLFLDESRRDGRLCVKLIHGKGLRSRAEGPVLKRLVDGMLRRRADVV
                     AFASAKPAEGGTGATVVLLKRL"
     gene            5137..>5314
                     /locus_tag="VFF05_04055"
     CDS             5137..>5314
                     /locus_tag="VFF05_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZW51010.1"
                     /translation="MRVLVSNDDGVDAPGILILAQRLSIVGEVTVVAPDRDRSGASNS
                     LTLDQPIRVMKLDER"
BASE COUNT          937 a         1698 c         1766 g          913 t
ORIGIN      
        1 ctgcagatgg acgtggcggg ccggctcaag gacatcacgg gcgagctcgg catcccgttc
       61 atcttcaagt cctcgtacga caaggcgaac cgcagctccg gcaagtcgtt ccgcgggctg
      121 ggcatggaga aggggctcga gatcctcgac aaggtgcgca ggtcgatcgg cgtgcccgtg
      181 ctcaccgacg tgcacgcgga agacgagatc tccgaggtcg ccctcgtggt ggacgtgctg
      241 cagacgccgg cgttcctgtg ccggcagacc gacttcatcc acgcggtcgc ctccgccggc
      301 aagcccgtga acctcaagaa gggccagttc ctgtcgccgt gggaaatgaa gcacgtcgcc
      361 gcgaaagcca aggcggtcgg caacgagcag atcatggtct gcgaacgcgg cgcgagcttc
      421 ggctacaaca acctcgtctc cgacatgcgc agtctcgcgg tgatgcgcga caccggctgt
      481 ccggtcgtgt tcgacgccac gcattcggtg caattgcccg gcggcgcgga tggcaagtcc
      541 ggcggccagc gcgaattcgt gcccgtgctc gcgcgcgcag cggtggcagt cggaatagcg
      601 ggcgtgttca tggaaacgca tcccgatccg gacaaggcgc tttccgacgg cccgaacgcg
      661 tggccgctgg gcaagatggc cgcgttgctc gaaaccctgg tcgcgctgga tcgcgtcgcg
      721 aaatcacaac ctctttagag ttcccccgat gagccaatcg atcacgaaaa tccacgcccg
      781 cgaaatcctc gattcgcgcg gcaatcccac gctggaagcc gaagtcacgc tggccgacgg
      841 ctcctttggc cgcgctgcgg tgccatcggg cgcctcgacc ggctcgcgcg aggcggtgga
      901 actgcgcgac ggcgacaagt cgcgctatct cggcaaaggc gtgaaaaatg ccgtcgccaa
      961 cgtcaatggc ccgattgcgc aggcgctgaa gggtcttgat gcggcggacc agaaaggcct
     1021 cgacgccaag ctcattgccc ttgacggcac gccaaacaag ggcaagctcg gcgcgaatgc
     1081 gctgctcggc gtttcgctcg ccaatgcgca cgcgatggcc gcgtcgaaaa aaatcccgct
     1141 gtggaaatat ctcgcgggtt cgcacgcgcc ttcgctgccg gtgccaatga tgaacatcgt
     1201 gaacggcggc gcgcacgccg acaacaacgt cgacatgcag gagttcatga ttcttccggt
     1261 tggcctgccg agcttcgccg aagcgctgcg cgcgggcgtg gaaatcttcc acgcgctcaa
     1321 atccgtgctc aaggccaagg gtctgaacac cgcggtcggc gacgagggcg gtttcgcgcc
     1381 ggacctgaaa tccaacgaac atgcgatcga ggtcatcctc gaagcgatcg ccaaggctgg
     1441 ttacaaggcc ggcaaagaca tttacctggg cctggatgcg gccagttccg aattcttcga
     1501 taacggcatg tacgacctgg ccggcgaggg caggaagatg tcgtccgccg agttgatcga
     1561 gttctatgcc ggctggtgcg cgaaatatcc gatcatcacc atcgaggacg gcgtcgccga
     1621 acaggatcgc gacggctgga aggcgctgac tgcgaagctc ggcaagaaaa tccagctcgt
     1681 cggcgacgac aacttcgtca ccaatccggc gatcttccgc gccggcatcg ccgacggcat
     1741 cgccaatgcc atcctgatca aggtcaacca gatcggcacc ttgagcgaaa cgctggaaac
     1801 gatcgcgatg gcggacaagg cgaattacgc ggcggtcgtc tcgcaccgct ccggcgaaac
     1861 cgaggacacc accatcgccg acattgcggt tgcgaccacg gcgacgcaga tcaagaccgg
     1921 ttcgctatgc cgttccgacc gcgtcgccaa atacaaccag ttgctgcgca tcgaggaagc
     1981 gctcggcgcg tccgcgaagt acgcgggccg cgcggcgttc ccgaacgtac cgggcttgat
     2041 cggctgagcg ttcgcgtgtg ctgcgttaca tcgccctcat cctgctgatc ctgctgatcg
     2101 cgttgcagat taagttctgg accggcgagg gcggcatgcg cgacgtgtgg cgcctgcagg
     2161 aacgcattgc cgaacagacg gccgagaatc ggaaactcaa gcagcgcaac gaggcgctgt
     2221 ccgccgaagt gcaggatctg aaatccggca agggcgcgat cgaggaacgt gcgcgttccg
     2281 aacttggcct gatcaagccc ggcgaggtgt tctatcaagt cgtggaaccc gcgcccgcga
     2341 atcccaagcg tgacaatggc aaccactgac gcgctgtggt gcgtggtgcc ggcggcgggg
     2401 cgcggcgcgc gcgtcggcgg cgaagtgccc aagcaatacc tgccgattgc cggcaagccg
     2461 atgctgttgc acacgctgga gcgcctggcc gcgcatccgc gtgtcgccgg actgatggtg
     2521 gtgctggagg ccggcgacgt gcgctggccg aagtggctca cgttcaacga caagccggtg
     2581 agcacgacga ttggtggcgc cgcacgcgcg gattcggtgc tggccggatt gcgcgcgctg
     2641 cccgaacaag tagataaatc ccattttgta cttgtccacg acgcggcgcg gccctgcgtg
     2701 cgtgtggcgg acgtttcgca cctgcttgaa ctgggcattc ccgcgggcgg cgcgttgctc
     2761 gccgcgccgg tgcgcgacac cctcaagcgc gcggacgcgc aggggcgggt agttgccacc
     2821 gagccacgcg aggcgcgctg gcgcgcgatg acgccgcaac tgttccgcct cggcgaactt
     2881 atgcaggcac tggaatccgc gcgcgacgcg ggcatcgccg tcaccgacga agccatggcg
     2941 atggaacgcg tcggccactc ccccttgctc gtcgaaggca gcgaggacaa tatcaaggtc
     3001 accaccgccg cggacttcgc gttggcgcag ttcattttgt cgaggacgga ttgatgcgta
     3061 tcggtcaggg aattgatatc cacgccttcg ggtccggcga tcacgttgtc gtcggcggcg
     3121 tgcgcatcgc acacacacgc ggtgtggtcg cgcattcgga cggcgacgtc gccatccacg
     3181 cgctgtgcga cgccatcctc ggcgcgctgg cgctcggcga tatcggccag cattttccac
     3241 cgacggatgc gcattggcgc ggcgccgaca gccgcgtgtt cctgcgccac tgcgcaggcc
     3301 tgatgcgcga acgcggattc gcgttaggca atgccgacat cagcatcgtc tgcgaggcgc
     3361 caaagatcgc gccgcacgct gtggcgatgc gcgcgaatct cgccgtcgat ctggcttgcg
     3421 acgttggcca ggtcagcgtc aaggcgacga ccagcgaaaa actcggcttc accggccgcg
     3481 gcgaaggcat cgccgcgctt gccgtcgtat tgctgaccgc ggcctgagat ggatttgccc
     3541 tgcgcccacg gcggcgcaac gttgcgcggc atcctgcggg ctacccccga ggatttcttc
     3601 gtcgatgaag agctgggctt cgcgccggat gctgcgggcg agcacgtctt cgtgcgcgtg
     3661 gaaaagcgcg gtgcgaacac ggatttcgtg gccagggaac tggcgcgttt cgctggtgtg
     3721 cgtccggatt cggtcagtta cgccggcatg aaggatcgcc acgccgtcac ccggcaaacc
     3781 ttttcgatcc acctgccggg caaggccgat ccggattggg cctcgctcgc gcatgcggaa
     3841 ttccgcgtgc tcgaagcggc ccggcattca cggaagttgc atcgcggcgc gctcaagggc
     3901 aatcgtttcc gcatcgtgct gcgcgaggtc gaaggcgacc gcgatgccgc ggagcgaatc
     3961 gtcgaatcgg ttcggacaca aggcgtcccc aactacttcg gcgaacagcg cttcggccgc
     4021 gaagcgtcga atgtcgagaa agcccgggcc atgttccagg gtaggcgcgt gcagcgccac
     4081 gaacgctcgc tgtttctttc cgccgcgcgc tcgcagatgt tcaacgccgt gctcgccgag
     4141 cgcgtcgaaa aaggcaactg gaaccgtccg ctcgacggcg atgtgttcat gctcgccggc
     4201 acgcacagca tcttcggccc ggagccgctg acgccggaat tggtagcgcg tcttgagcgc
     4261 ggcgacatcg cgccaaccgg gccgatgtgg ggcgccggcg atttgcgctc gcgcgatgcg
     4321 gtggcggaac tggaaaattc cgtcgccgcg cggtatcccg atctggctgc gggactttcc
     4381 gtcgcgggac tgcgccagga acgccggagt ctggtcttgc gtccgcgcga gctggaattg
     4441 ggctggcttc aggactccgc cgccgagctg agtttttact tgagctctgg cgcttatgcg
     4501 actgttttat tgagagaaat attcgattat cgaaacccgg cctaaagccg tttcagcagc
     4561 acgacggttg cacctgtccc gccttcggcc ggcttggccg aggcgaaggc gaccacgtct
     4621 gcgcgccggc gcagcatgcc gtcgaccagg cgcttgagca ccggcccttc ggcacgcgag
     4681 cgcaagccct tgccgtggat cagcttcacg cacagccgcc cgtcgcggcg cgattcgtcc
     4741 aggaatagct tgaccgcttc gcgcgccacc gccgccgtca tttgatgcag gtcgatttcg
     4801 tcggcgatgg aaaagtgccc gcgcttgagc tgacgcagca ggcgcggcga atggccgtcc
     4861 ttgagatagc tcaattcctc gccgatctcg atcgccgccg ggtcgatggc gctgtcgagc
     4921 agttcatcgg ccacgctggc ctcgtcgcga tccgactggg ttggctcggg cgccggtttc
     4981 ggcgccgcgt cggcgcgttc gcgggcatgc aggcgccgca ccggcccgac cgcgtcggcg
     5041 aacaggcgca ggtcgtcttc gctgatggtg acggatttgg atttgcgcat gggccgagga
     5101 tacgtcagcg gtgcggcttg gtagaatagg cggcttatgc gagtactggt tagtaatgac
     5161 gacggcgtcg acgccccggg catcctgatc ctggcgcaac ggctttcgat cgtgggcgaa
     5221 gtcactgtgg tcgcgcccga ccgcgaccgt tccggcgcaa gcaattcgct gaccctggat
     5281 cagccgatcc gggtgatgaa gctcgacgag cgcc
//
LOCUS       DASRVO010000326         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_139256, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000326 DASRVO010000000
VERSION     DASRVO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139256"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1716
                     /locus_tag="VFF05_04060"
     CDS             <1..>1716
                     /locus_tag="VFF05_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZW51011.1"
                     /translation="GDDWFVHDIASGTTAPAAVLKAENDPDAAPKDDVLRAEQLRLFT
                     TLKKMDKDKKETREHAQAMQRGDASRAPLPFYLGDKVKLVDTELSPDTHWLLAVTVEK
                     NYDRGEQPKLIRYVTDTGYPEFEPLRRNAGRNAPAPQTLWLFDLAKHTQAKIDVDALP
                     GIHDDPLAAIRAENEKGGLLKTDSKKDESGKDGKSAKADKERMRDLEIAGIEFSRDGT
                     HAAVQLRAIDNKDRWIATLDPAHPTLALQHRLHDDAWINWNFNEFGWENDSRTLWYVS
                     EESGFAQLYAKPLDGKTRQLTHGNFEVSRPQLSTDGRWFYVRANAEEPWAYDVYRVGV
                     DGGDLQRVTHYQGMDSFALSHDGAKLLVVHSSSYVPPQLAVVDADGSDSAHELTDTRT
                     AAYKALNWVAPQFVQVPSTHFKGSVWAKFYAPADYDKSKPHPAVIFVHGAGYLQDVNA
                     NWSYYFREQMFNNLLLQHGYVVIDLDYRASEGYGRDWRTAIYRQMGHPELEDLLDGKA
                     WLVKNHGVDPKRVGIYGGSYGGFMTEMALLRAPGEFAAGAALRAPSDWTSYNDEYTSN
                     ILNDP"
BASE COUNT          372 a          566 c          522 g          256 t
ORIGIN      
        1 cggcgacgac tggttcgtgc acgacatcgc gagcggaacg acagcgccgg ccgcggtgct
       61 gaaagccgag aacgatcccg atgccgcgcc caaggacgac gtgctgcgcg ccgaacagct
      121 gcgcctgttc acgacgctga aaaaaatgga caaggacaag aaggaaaccc gcgaacacgc
      181 gcaggcgatg cagcgcggcg atgcgagccg cgcgccgctg ccgttttatc tcggcgacaa
      241 ggtcaagctg gtcgatacgg aactttcgcc cgacacgcac tggctgctcg cggtcacggt
      301 ggaaaagaac tacgaccgcg gcgagcagcc caagctgatc cgctacgtca ccgacaccgg
      361 ctatccggaa ttcgaaccgc tgcgccgcaa cgccggtcgc aacgcgcccg cgccgcagac
      421 gttgtggctg ttcgatctgg ccaagcacac gcaggccaag atcgacgtcg acgccctgcc
      481 cggtatccac gacgacccgc tcgcggcgat ccgcgcggag aacgagaagg gcggcctgct
      541 gaagaccgat tcgaaaaaag atgaaagtgg aaaggacggc aaatccgcca aggcggacaa
      601 ggagaggatg cgcgatctgg aaatcgccgg catcgagttc agccgcgacg gcacgcacgc
      661 cgccgtgcag ttgcgcgcaa tcgacaacaa ggatcgctgg atcgcgacgc tcgaccccgc
      721 gcatccgacg ctcgcgctgc agcaccgcct gcacgacgac gcctggatca actggaattt
      781 caacgaattc ggctgggaaa acgacagccg cacgctttgg tacgtgtccg aggaatccgg
      841 cttcgcgcag ctctacgcca agccactgga cggcaagaca cgccagctca cgcacggcaa
      901 cttcgaagtg tcgcgtccgc aactttcaac cgacggacgc tggttctacg tacgcgcgaa
      961 tgcggaagaa ccctgggcct acgacgtgta tcgcgtcggc gtcgacggcg gcgatttgca
     1021 gcgcgtcacg cattatcaag gcatggattc gttcgcgctg tcgcacgacg gcgcgaagct
     1081 tctggtcgtg cattcgtcgt cctacgtacc gccgcaactg gccgtcgtcg atgccgacgg
     1141 cagcgacagc gcgcatgaac tcaccgacac gcgcaccgcg gcctacaaag ctttgaactg
     1201 ggtcgcaccg cagttcgtgc aggtgccgtc cacgcacttc aagggctcgg tctgggccaa
     1261 gttctatgcg cctgccgact atgacaagtc gaaaccgcat ccggcggtga ttttcgtgca
     1321 cggcgccggt tatctgcagg acgtcaacgc gaactggtcg tactacttcc gcgagcagat
     1381 gttcaacaac ctgctgctgc aacacggtta cgtcgtcatc gacctggatt accgcgcatc
     1441 cgaaggctac ggccgcgact ggcgcaccgc gatctatcgc cagatgggcc atcctgaact
     1501 ggaagacctg ctcgacggca aggcatggct ggtgaaaaac cacggcgtcg acccgaagcg
     1561 cgtgggcatc tacggcggca gctacggcgg cttcatgacc gagatggcac tgctgcgcgc
     1621 accgggcgaa ttcgccgccg gcgccgcgct gcgcgcacca tcggactgga ccagctacaa
     1681 cgacgaatac acctccaaca tcctcaacga cccgca
//
LOCUS       DASRVO010000327         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_243454, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000327 DASRVO010000000
VERSION     DASRVO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243454"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /gene="rplW"
                     /locus_tag="VFF05_04065"
     CDS             <1..108
                     /gene="rplW"
                     /locus_tag="VFF05_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145338.1"
                     /note="binds third domain of 23S rRNA and protein L29;
                     part of exit tunnel; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZW51012.1"
                     /translation="DKTKSFRFRAGSRKGWRKAYVRLSEGQTIDVTAKP"
     gene            142..928
                     /gene="rplB"
                     /locus_tag="VFF05_04070"
                     /pseudo
     CDS             142..928
                     /gene="rplB"
                     /locus_tag="VFF05_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009607340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            932..1213
                     /gene="rpsS"
                     /locus_tag="VFF05_04075"
     CDS             932..1213
                     /gene="rpsS"
                     /locus_tag="VFF05_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZW51013.1"
                     /translation="MARSLKKGPFVDLHLLKKVEAAASSNSKKPIKTWSRRSMILPEM
                     VGLTIAVHNGKAHVPVLVNENMVGHKLGEFAATRTFKGHSADKKAEDKK"
     gene            1240..1596
                     /gene="rplV"
                     /locus_tag="VFF05_04080"
     CDS             1240..1596
                     /gene="rplV"
                     /locus_tag="VFF05_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZW51014.1"
                     /translation="MQAKATENAQQAKAILRGVRISPQKARLVADQVRGLPVGRATDL
                     LKFSDKKAAHLVKKVLLSAVANAENNLGADVDELKVATIMVDEGPRLKRMHARAKGRG
                     SQIIKRTSHITVVVGS"
     gene            1607..2353
                     /gene="rpsC"
                     /locus_tag="VFF05_04085"
     CDS             1607..2353
                     /gene="rpsC"
                     /locus_tag="VFF05_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZW51015.1"
                     /translation="MGHKVHPTGIRLGISKDWNSKWFATKREYAQYLAADLKVRELLH
                     AKLAQAGVSKIQIERPAKTARITIHTARPGVVIGKKGEDIEKLRKEVTKMMGVPAHIN
                     VAEVRKPELDAQLVAESIAQQLERRIMFRRAMKRAVGNAMRLGALGIKVNVGGRLNGA
                     EIARSEWYREGRVPLHTLRADIDYGFAQAKTTYGIIGVKVWVYKGEVFDLHAASQETK
                     EEARDSAPPSRERREPREHREPRGGREARN"
     gene            2357..>2716
                     /gene="rplP"
                     /locus_tag="VFF05_04090"
     CDS             2357..>2716
                     /gene="rplP"
                     /locus_tag="VFF05_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZW51016.1"
                     /translation="MLQPKRTKYRKVQKQRNRGLSFVANKVSFGEFGLKATTHGHLTA
                     RQIEAARRCVTRFVKRGGKLWIRVYPDKPISKKPIEVRMGNGKGNVEFWVAVVQPGRM
                     LYEIEGVTEAEAREAFRL"
BASE COUNT          592 a          870 c          866 g          388 t
ORIGIN      
        1 gacaagacca agtcgttccg cttccgcgcc ggcagccgca agggctggcg caaggcttac
       61 gtgcgccttt ccgaaggcca gaccatcgac gtgacggcaa aaccgtaagc gccgcgccgt
      121 atccatcggg aaatcaagac aatggcactg attacagtca agcccacttc cgccggacgc
      181 cgcgccatgg tgcgcgtgtc gacgccgggg ctgtacaagg gcgatccgta cgcgccgctc
      241 accgagtcgc aggcgaagac cggcggccgc aaccactatg gccgcatcac cacgcggcac
      301 aagggcggcg gttcaaagca gaattaccgc atcatcgact tcaagcgtga caaggaaggc
      361 atcgcctgcc gcgtcgagcg cctggagtac gatccgaacc gcaccgcgca catcgcgctg
      421 ctgtgctacg tcgacggcga acgccgctac atcattgcgc cgaagggcgt ggcgatcggc
      481 gaccagctga tggccggccg cgatgcgccg atcaaggcgg gtaacacgct gccgttgcgc
      541 aacatcccga tcggctcgac gatccactgc atcgagatga agcctggcaa gggcgcgcag
      601 cttgcgcgca gcgccggcgc ttcggtgcag ctggtcgcgc gcgaaggcgg ctatgccacc
      661 ttgcgcctgc gctccggcga aatgcgcaag gtgccggccg actgccgcgc cacgatcggc
      721 gaagtcggca actctgaaca caacctgcgc aagctcggca aggccggtgc caagcgctgg
      781 cgcggcatcc gcccgaccgt tcgcggcgcg gcgatgaacc cggtcgacca tccgcacggt
      841 ggtggcgagg cgaaagccgg ccaagggtta caagacgcgc aagaacaagc gcaccaccca
      901 gttcatcgtg cgcgatcgca gaggctaatc catggcacgt tcactgaaaa aaggcccgtt
      961 cgtcgacctg catcttttga agaaggtcga ggccgcggct tccagcaaca gcaagaagcc
     1021 gatcaagacc tggtcgcgcc gttcgatgat cctgccggaa atggtgggcc tgacgatcgc
     1081 cgtgcacaac ggcaaggcgc acgtaccggt tctggtcaac gagaacatgg tcgggcacaa
     1141 gctcggcgaa ttcgccgcca cgcgcacctt caagggccac tcggccgaca agaaggccga
     1201 ggacaagaag tagagccgat ttcataaggt atgacgatga tgcaagccaa agccacagaa
     1261 aacgcgcagc aggccaaagc gatcctgcgc ggcgtgcgca tttcgccgca gaaagcccgc
     1321 ctggtcgccg accaggtgcg tggactgccg gtcggacgcg ccacggacct gctcaagttc
     1381 agcgacaaga aagccgcgca tctggtaaag aaggtgttgc tgtcggcggt cgccaatgcc
     1441 gagaacaatc tcggcgccga cgttgacgag ctcaaggtcg ccacgatcat ggtcgacgaa
     1501 ggcccgcgcc tgaagcgcat gcacgctcgc gccaagggcc gcggctcgca aatcatcaag
     1561 cgtaccagcc acatcaccgt ggtcgtgggc agctgaggaa aacacgatgg gtcataaagt
     1621 tcatccaacc ggtatccgcc tcggcatttc caaggactgg aattcgaagt ggttcgccac
     1681 caagcgcgaa tacgcgcagt acctggccgc cgatctgaag gtacgcgagt tgctgcacgc
     1741 caagctcgcc caggccggtg tgtcgaagat ccagatcgag cgtccggcca agaccgcgcg
     1801 catcaccatc catacggcgc gaccgggtgt ggtgatcggc aagaagggcg aggacatcga
     1861 gaagctgcgc aaggaagtga ccaagatgat gggcgtgccc gcccacatca acgtcgccga
     1921 agtgcgcaag cccgaactcg acgcgcagct ggtggccgag tccatcgccc agcagctcga
     1981 gcgccgcatc atgttccgcc gcgcgatgaa acgcgccgtg ggcaatgcga tgcgcctcgg
     2041 cgcgctcggc atcaaggtca atgtcggtgg ccgtttgaac ggcgccgaga tcgcgcgttc
     2101 ggagtggtac cgtgaaggtc gcgtgccgct gcatacgctg cgcgcggaca tcgattacgg
     2161 cttcgcccag gcgaagacca cgtacggaat catcggcgtc aaggtgtggg tatacaaggg
     2221 cgaagtgttc gacctgcacg ccgccagtca ggaaaccaaa gaagaagccc gcgacagcgc
     2281 gccgccctcg cgcgagcgcc gcgagccgcg tgagcaccgc gaaccccgcg gcggtcgcga
     2341 agcaaggaat taagccatgt tgcaacccaa acgcaccaag taccgtaagg tccagaagca
     2401 gcgcaatcgc ggcctgagct tcgtcgcgaa caaagtgagc tttggcgagt tcggcctgaa
     2461 ggcgaccacg cacggtcacc tgaccgcgcg ccagatcgaa gccgcgcgtc gttgtgtcac
     2521 gcgcttcgtc aagcgcggcg gcaagctctg gatccgcgtc tatccggaca agccaatcag
     2581 caagaaaccc attgaagtcc gcatgggcaa cggcaagggt aatgtcgagt tctgggtcgc
     2641 cgtcgtgcag cccggccgca tgctctatga aatcgagggc gtcaccgagg ccgaggcgcg
     2701 cgaggcgttc cgtctg
//
LOCUS       DASRVO010000328        10051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_296427, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000328 DASRVO010000000
VERSION     DASRVO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296427"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..974)
                     /locus_tag="VFF05_04095"
     CDS             complement(105..974)
                     /locus_tag="VFF05_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZW51017.1"
                     /translation="MNASAAPRRVELLQVRAPNVPSFVLALETQGDRSIAPHLRAHGV
                     WEPAETALCQRLLRPGMRVVDAGAHAGYYSVLFSHLVGATGGVIAFEPEPANFRMLLA
                     NLLLNDCGNVEARPLALAEGRGAEFLHLCDDNPGDHRLAPVPGRARIEVETIDLDSTL
                     AGTPLDFIKIDTQGAEPRILAGMANTISVNRERLGCLMEFAPGLLQLAGVSLDQYAAQ
                     LAALDARVFAVSLRNHDLNLQRLVPLRVGLDMLVDLLAPLGEVDASCDVFAVFGAAAE
                     AAWLARFQQGWVG"
     gene            complement(971..1624)
                     /locus_tag="VFF05_04100"
     CDS             complement(971..1624)
                     /locus_tag="VFF05_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51018.1"
                     /translation="MNASAFRLLIGTPAYGGMMHSDYVSALLQYQRAGIDFALMSIGN
                     ESLITRARNTILSEFHARAEYSHLLFLDADVLLPPAGLRRMLAAGRDVIGAAVALKGR
                     NASGGRIFNVGAVRGEDGALYQVERIGTAALLLSRAAVAALVVDARREGRVYSRGFDL
                     RGVELKSSVQYDIFRTGVVGDEYLSEDYWVCRELRRLGFAVYLDPTIVTRHSGMMEA"
     gene            complement(1621..2415)
                     /locus_tag="VFF05_04105"
     CDS             complement(1621..2415)
                     /locus_tag="VFF05_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyltransferase-related protein"
                     /protein_id="HZW51019.1"
                     /translation="MQKIAYILTWRDGEDASRRANLFAVLEWLARNPQFEPFLVEQDS
                     VPRLDGGLPHPACKHVFAYNPGPFNKSWGLNVGFRCCTHAWLAFADADIVLGAALTAT
                     LEHLEKGYWAIKPYRRLIDLDASESARVRGGDFDFVPDRRDAAPNREGAGERIVFAGG
                     VFLIARAAFVRLGGWDERFRGWGGEDDAFSYRLERARVPAFELDIRAAVHLAHPRSQE
                     TTYAQPHYAANCALLESYRQMEDPQLERLSEIQAQIVGNRDKYRPE"
     gene            2581..3345
                     /locus_tag="VFF05_04110"
     CDS             2581..3345
                     /locus_tag="VFF05_04110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZW51020.1"
                     /translation="MISCLMITQASRLALAKLAIGDFAAQTHAERELVVVHDGDDTCH
                     AQLSGLVAKLQTAPIRCVRHAPGSTLGALRNASVDAAHGEFVCQWDDDDRYHPQRLAL
                     QWRALNEANADFSYLCDQLHWFPARGEMYWDDWNREAYPLNVVQGSLLGRRERMPRYA
                     QLARGEDTDVLLKILRQGSAIARVRDAGWSSVYVYHGANTFDGAHHAAIARAKALGHA
                     ALAQRETLLRQRLTEYAPPLGALRMPHTGGALQFDG"
     gene            complement(3342..4178)
                     /locus_tag="VFF05_04115"
     CDS             complement(3342..4178)
                     /locus_tag="VFF05_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW51021.1"
                     /translation="MSARRDFHFVFGLREQREPFHILHYLCLASCRAVNQPDAIHFHY
                     RHEPFGPWWERIKPHLRLVRAPDVVEGFAPARYADSAEGRFIEQLGLSYAHEADFLRL
                     DILIAHGGVYCDMDTLFVRPYADAWFAEDFAIGEESAPFARGQVIRPTLCNAVMFARP
                     ASRFARVWRERMEQVFDGSWSGHSCHEAARLWRVMPDALRVLPREYFYRYGSTIAGLE
                     SLLEQSDAEHADIYSMHLWAHLWWERSRRDFSAVHAGQIDENWIRRRDCTLAKLARKF
                     LD"
     gene            complement(4175..5389)
                     /locus_tag="VFF05_04120"
     CDS             complement(4175..5389)
                     /locus_tag="VFF05_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51022.1"
                     /translation="MPASPRGVLYCGSGDLSGYGQAAIAYVRALVNAGIPVQWVPLDW
                     LPERMAVGSWTLPDGRPRGLLERCGTYGHLADLPVLVERTRAPVAHDTVVVHAPPEAW
                     PGIFENGKRNIGVTVWETDRAPAHWLPLMRQADRVIVPCEFNRAAFVRSGLDRPIHVV
                     PHIRRHTWREHTPADIARARAQLGIAPGQRVFYTINDWGPRKNMPALIRAFARAFAAQ
                     APLSLLIKTAAIGHDGPPFYPNVPTRELVLQAMRDATAEVWRPLPQIVLVDAELDGDG
                     IDLIHAIGDVYVSLTHAEGWGLGAFEAATLGKPVLMTAWGGQCEFLGADWPGAIPCAL
                     EPVPVWPPHQPSYFPSQCWATPDADAAVALMRAAADDDAPMQVAANAIRERIVRDYAE
                     PVVVDALIEAIA"
     gene            complement(5392..6483)
                     /gene="ribB"
                     /locus_tag="VFF05_04125"
     CDS             complement(5392..6483)
                     /gene="ribB"
                     /locus_tag="VFF05_04125"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZW51023.1"
                     /translation="MSFATIPELVADIRAGRMVVIVDDEDRENEGDLIMAAQCVRPED
                     VNFMVTHARGLVCLSLTRERCAQLRLPPMVSANASRHHTNFTVSIEAAAGVTTGISAH
                     DRAHTIRTAVGADTKPADLVQPGHIFPLAAQPGGVLTRAGHTEAASDLAALAGCEPAG
                     VLVEILAEDGQMARRPQLEVFAARHGLKIGSIADLIRYRLATERTVVRMAEREVETAF
                     GAFRLIGYRDALTGLAHFALVRGRIDDGAAVLARVHVRDALADALQLARADHGVTLDA
                     ALRRVDQVGRGVIVLLTEADEAANALERLVQPAQPRSDDEGEWRRHGIGAQILADLGA
                     RRLIVLGTKRRFSGLSGFGLEVVGYQEAG"
     gene            complement(6480..7076)
                     /locus_tag="VFF05_04130"
     CDS             complement(6480..7076)
                     /locus_tag="VFF05_04130"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZW51024.1"
                     /translation="MFTGIVQATGIVTRSEARGGDARLFVDTAALDVELNLGDSLCVS
                     GVCLTVVAREGGIHAFDVSVETLALTTLAALHVGDAVNLEPALRLSDRLGGHLVSGHV
                     DGIGRVVAIEHDARSQRWTFEVPAPLSRYIAAKGSVCIDGVSLTVNDVRGNRFGVNLI
                     PHTVDVTAFRGKRVGDAVNIEVDLIARYVERLRAGETA"
     gene            complement(7081..8178)
                     /gene="ribD"
                     /locus_tag="VFF05_04135"
     CDS             complement(7081..8178)
                     /gene="ribD"
                     /locus_tag="VFF05_04135"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZW51025.1"
                     /translation="MNGFSAADHAHMARALHLAEKGLYTTQPNPRVGCVIARDDEILG
                     EGFHARAGEPHAEVFALRAAGMCARGATAYVTLEPCAHFGRTPPCADALVAAGVARVV
                     AACEDPNPKVAGGGIAKLRAAGIAVETGLLRGAARELNRGFISRLERGRPWVRVKLAM
                     SLDGRTALANGQSQWITSEAARADVQRWRARSSAILTGAGTARVDDPRLTVRLADGEA
                     FVPALRVVLDARLDALPAQARLLDGSTPTLILHAEGAQPCDDRYAGVERVAVAAQADG
                     GFDLDAALALLAARGVNELQVEAGPTLCGALFAANRVDELLLYVAPILLGDGARALLN
                     LPPIAEIAAGRRLRVVDERRIGEDWRLLLRE"
     gene            complement(8175..8693)
                     /gene="nrdR"
                     /locus_tag="VFF05_04140"
     CDS             complement(8175..8693)
                     /gene="nrdR"
                     /locus_tag="VFF05_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZW51026.1"
                     /translation="MHCPFCQHVDTRVIDSRESEDGTTIRRRRECTHCGERFNTFETM
                     EIKLPAIVKSDGRREAFGEHKLRTGVERALQKRPVASDAVDNVVREVVRQLRAVNERE
                     VPSRLLGEWVMTELKRLDQVAYVRFASVYRRFEDVQAFREEIEKLEKDLPELEGLQLP
                     LLGDLRQRKKAP"
     gene            complement(8740..10008)
                     /gene="glyA"
                     /locus_tag="VFF05_04145"
     CDS             complement(8740..10008)
                     /gene="glyA"
                     /locus_tag="VFF05_04145"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZW51027.1"
                     /translation="MFAATMKIEGYDPELAAAIAAERQRQEDHVELIASENYASPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDVAERLAIERLKQLFDCDYANVQPHSGSQAN
                     QAVYFALLNPGDTILGMSLAHGGHLTHGAKVNLSGKILNAVQYGVNADGFIDYGEVEK
                     LAVVHRPKMVVAGFSAYSQVVDWARMRAIADKVGAYLFVDMAHVAGLIAAGAYPSPLP
                     HAHVVTSTTHKTLRGPRGGLIVAKHSAMGPAAEEIEKKLQSIVFPGIQGGPLMHVIAA
                     KAVAFKEALEPAFKTYQHNVVKNAQAMAKTIVARGYKIVSGGTMNHLMLVDMIAKGVT
                     GKDAEAALGRAHITVNKNAVPNDPQKPFVTSGLRIGTPAVTTRGYGEADCVELANWIC
                     DVLDAPADDKVIAAVREKVTAQCRKFPVYG"
BASE COUNT         1744 a         3366 c         3320 g         1621 t
ORIGIN      
        1 gcggctggcg acaatggcga accgcgcttg cgcatcggcg cgtggatcgc cggcgaattc
       61 cggcacgggc gtttcctctt cgcggcgggc gccgcgcgcg tagtttaccc gacccagcct
      121 tgctgaaacc gcgccagcca cgccgcttcc gcggcagcgc cgaagaccgc gaacacgtcg
      181 caggaggcgt cgacctcgcc cagcggcgcc agaaggtcga cgagcatgtc cagaccgacg
      241 cgcagcggca ccaggcgctg caggttgaga tcgtgattcc gcagcgaaac cgcgaacacg
      301 cgtgcgtcca gggcagccag ttgcgcggca tactggtcga gcgaaacgcc ggcgagttgc
      361 agcagtcccg gcgcgaattc catcaggcaa cccagacgct cacggttgac tgaaatggtg
      421 ttcgccatgc cggcaaggat gcgcggctcg gcgccctgcg tgtcgatctt gatgaaatcc
      481 agcggcgtgc cggcgagcgt ggaatccagg tcgatggtct cgacctcgat acgcgcgcgt
      541 cccggcaccg gcgcgagacg gtgatcgccc gggttgtcgt cgcacaaatg caggaattcg
      601 gcgccgcggc cttccgcgag cgccagtggc cgcgcctcga cattgccgca atcgttgagc
      661 agcaggttgg ccagcagcat gcggaaattg gccggctccg gctcgaaggc gatgacgccg
      721 ccggtcgcgc cgaccagatg cgagaacaac acgctgtagt aaccagcgtg cgcgccggca
      781 tcgaccacgc gcatgcccgg gcgcagcagg cgctggcaca gggcggtctc ggccggctcc
      841 cagacgccgt gagcgcgcag atggggtgcg atgctgcgat cgccctgcgt ttccagcgcg
      901 agaacgaacg acggcacatt cggcgcgcgc acctggagca actcgacgcg acgcggcgcc
      961 gccgacgcgt tcacgcctcc atcatcccgg aatggcgggt gacgatggtc ggatccaggt
     1021 acacggcgaa tccgagccgg cgcagctcgc ggcagaccca gtaatcctcg gacaggtatt
     1081 cgtcgccgac cacgccggtg cggaagatgt cgtactgcac gctgctcttg agttccacgc
     1141 cgcgcaggtc gaagccgcgc gagtacacgc gcccttcgcg gcgcgcatcg accaccagcg
     1201 cggcgaccgc cgcacgcgac agcagcagcg cggccgtacc gatgcgctcg acctggtaca
     1261 gcgcgccatc ttcgccgcgc accgcgccga cattgaagat gcgcccgccg ctggcgttgc
     1321 ggcccttgag cgcgaccgct gcaccgatga cgtcgcggcc cgcagccagc atgcggcgca
     1381 acccggcggg tggcagcagc acgtccgcat cgaggaacag caggtggctg tattcggcgc
     1441 gggcgtggaa ttcggaaagg atggtgttgc gtgcgcgcgt gatcaggctt tcgttaccga
     1501 tgctcatcag cgcgaagtcg atgccggcgc gctggtactg caacagcgcg ctcacatagt
     1561 cgctgtgcat catgccgcca tacgccggcg tgccgatcag cagacggaat gcggaggcgt
     1621 tcattcgggt cggtacttgt cgcggttgcc gacgatctgc gcctggattt ccgacagccg
     1681 ctccagttgc ggatcttcca tctgacgata gctttccagc agcgcgcagt tggcggcgta
     1741 atgcggttgg gcgtaggtgg tttcctgcga acgcggatgc gccaaatgaa ccgccgcgcg
     1801 aatatcgagt tcgaaggcag gcacgcgcgc gcgttcgagg cggtagctga aagcatcgtc
     1861 ttcgccgccc cagccgcgga agcgttcgtc ccagccgccc aagcgcacga acgcggcgcg
     1921 cgcgatgagg aacacgccgc cggcgaacac gatgcgctcg cccgcaccct cgcggttggg
     1981 cgcggcatcg cggcgatcgg gaacgaaatc gaaatcgccg ccgcgcacac gcgcgctttc
     2041 ggacgcgtcg aggtcgatca ggcgacgata cggtttgatc gcccaatagc ccttctccag
     2101 atgctccagc gtggcagtca aggcggcgcc aagcacgatg tcggcatcgg caaaggccag
     2161 ccaggcgtgc gtacagcagc ggaagccgac gttcaggccc cagctcttgt tgaacggtcc
     2221 cgggttgtag gcgaatacat gcttgcaagc cggatgcggc aaaccgccat ccaggcgcgg
     2281 caccgagtcc tgctcgacga gaaacggctc gaactgcgga ttacgcgcca gccattccag
     2341 cacggcaaaa agattggcgc ggcggctcgc gtcttcgccg tcgcgccaag tcaggatgta
     2401 agcgatcttt tgcatctatc ttgagaacct atctcaaaat cgtcgcgagc gaagtcaggt
     2461 tgcgcgccgc cggagcgcag gaaccggagt ttactggtag taaatgagga ttccgagcac
     2521 cgccggcccg cgagatgacg agcgcagcag attttgggat tggttctaca atctggcgcc
     2581 atgatcagtt gcctgatgat aacccaggcc tcgcgcctcg cgctggcgaa actcgcgatc
     2641 ggcgatttcg cggcgcagac gcacgccgaa cgcgaactgg tagtcgtgca cgatggcgac
     2701 gatacctgtc acgcacagtt gagcgggctc gtcgcgaagc tgcaaacggc gccgatccgc
     2761 tgcgtacggc acgcacccgg atcgacgctc ggcgccctgc gcaatgcgtc ggtcgatgcg
     2821 gcgcatggcg aattcgtctg ccaatgggac gacgacgacc gctaccatcc gcaacgcctg
     2881 gcattgcagt ggcgcgcgtt gaacgaagcg aacgcggact tcagttatct gtgcgaccag
     2941 ttgcactggt tcccggcgcg cggcgagatg tattgggacg actggaaccg cgaagcgtat
     3001 ccgctgaacg tggtgcaggg ttcgctgctc ggccggcgcg aacgcatgcc gcgttacgcg
     3061 caactcgcgc gcggcgagga taccgacgtg ctactgaaga tcctgcgcca gggcagcgcc
     3121 atcgcgcgcg tgcgcgacgc cggctggagt tcggtctacg tctaccacgg cgccaacacg
     3181 ttcgacggcg cgcatcatgc ggcgatcgcg cgcgccaagg cgctgggcca cgcggcgctg
     3241 gcgcaacgcg aaacactact acgtcaacgt ctgaccgaat acgcgccgcc gctcggcgct
     3301 ctgcgcatgc cgcatacggg cggcgctctg caattcgacg gctaatccag aaatttacgc
     3361 gccagcttcg ccagcgtgca atcgcgccta cggatccagt tttcgtcgat ttgcccggcg
     3421 tgcactgcgg aaaaatccct gcgcgaccgc tcccaccaca ggtgcgccca cagatgcatg
     3481 ctgtagatgt cggcatgctc ggcgtcggac tgctcgagca ggctttccaa tccggcaatc
     3541 gtgctgccgt agcggtagaa gtactcgcgc ggcagcacgc gcagggcatc cggcatgacc
     3601 cgccacagcc gcgcggcttc gtggcaggaa tgcccgctcc agctgccatc gaagacttgc
     3661 tccatgcgct cgcgccagac gcgggcgaaa cgcgacgcgg gccgcgcgaa catcaccgcg
     3721 ttgcacaggg tcggacggat gacttgtccg cgcgcgaacg gtgcgctttc ctcgccgatc
     3781 gcgaaatcct cggcgaacca tgcgtccgca tacggccgca cgaacagggt gtccatgtcg
     3841 cagtagacgc cgccgtgcgc gatgaggatg tccaggcgca ggaaatccgc ctcgtgcgca
     3901 tagctcaatc cgagctgttc gatgaagcgg ccttccgcac tgtccgcgta acgcgcgggc
     3961 gcgaaacctt caacgacatc cggggcacgc accagccgca gatgcggctt gatcctttcc
     4021 caccatggac cgaatggctc gtgccggtaa tggaaatgga ttgcgtccgg ctggttcacc
     4081 gcgcggcacg acgccaggca caggtaatgg aggatgtgga acggctcgcg ttgctcgcgc
     4141 aggccgaaga cgaagtggaa gtcgcgccgt gcgctcatgc gatggcctcg atcaatgcgt
     4201 cgacgacgac cggttcggca tagtcgcgca cgatgcgttc gcggatggcg ttcgccgcaa
     4261 cctgcatcgg agcgtcatcg tccgccgccg cgcgcatcag cgccacggcg gcgtccgcat
     4321 cgggcgtcgc ccagcactgg ctcgggaaat acgaaggctg gtgcggcggc cacaccggca
     4381 ccggctcgag cgcacaagga atcgccccgg gccagtcagc gccgagaaat tcgcactgcc
     4441 cgccccaggc tgtcatcagc acgggcttgc cgagcgtggc cgcctcgaac gcgcccaggc
     4501 cccagccttc ggcatgggtg agcgagacat aaacatcgcc gatggcgtgg atcagatcga
     4561 tgccgtcgcc gtcgagttcc gcatcgacca gcacgatctg cggcaaaggc cgccagactt
     4621 cggccgtggc gtcgcgcatg gcctgcagga cgagttcgcg cgtgggcacg ttcggataaa
     4681 agggtgggcc gtcgtgcccg atcgcggcgg tcttgatcaa caggctcagc ggcgcctgtg
     4741 cggcgaaggc gcgcgcgaat gcgcgaatca acgccggcat gttcttgcgc gggccccagt
     4801 cattgatcgt gtagaacacg cgctggccgg gcgcgatgcc gagttgcgcg cgcgccctgg
     4861 cgatgtccgc cggggtgtgt tcgcgccagg tgtggcggcg gatgtgcggc accacgtgga
     4921 ttggcctgtc caacccgctg cgcacgaacg ccgcgcggtt gaattcgcag ggcacgatca
     4981 cgcgatcggc ctggcgcatc agcggcaacc agtgcgccgg cgcgcgatcg gtctcccaca
     5041 cggtaacgcc gatgttgcgc ttgccgttct cgaaaatccc gggccaggcc tccggcggcg
     5101 cgtgcacgac caccgtgtcg tgcgcgaccg gcgcgcgcgt gcgctcgacc agcacgggca
     5161 aatccgccag atggccgtac gttccgcaac gttcgagcag accacgtgga cggccatccg
     5221 gcagcgtcca cgaaccgacc gccatgcgct cgggcagcca atccagcggc acccactgca
     5281 ccggtatgcc ggcattgacc agcgcgcgca cataggcaat ggcggcctgg ccgtaaccgc
     5341 tcaggtcgcc ggaaccgcag tacagcacgc cgcgcgggct tgccggcatc gctacccggc
     5401 ctcctggtag ccgacgactt ccagaccgaa tccggatagc ccggaaaacc ggcgtttggt
     5461 gccaagcacg atcaggcgcc gcgcgccgag atcggcgagg atctgcgcgc cgatgccgtg
     5521 gcggcgccac tcgccttcat catcggatcg cggttgcgcc ggttgcacga ggcgttcgag
     5581 cgcgttcgcc gcttcgtccg cctcggtcag cagcacgatg acgccgcggc cgacctggtc
     5641 gacgcggcgc agcgccgcgt ccagggtcac gccgtgatcg gcgcgcgcaa gttgcagcgc
     5701 atcggcgagc gcgtcgcgca catgcacgcg cgccagcacg gccgcaccgt cgtcgatgcg
     5761 gccgcgcacc agggcgaaat gcgccagacc cgtgagtgca tcgcgataac cgatgagtcg
     5821 aaatgcgccg aacgcggtct ccacctcgcg ttcggccatg cgcacgacgg tgcgttcggt
     5881 cgcgagccgg taccggatca ggtcggcgat ggagccgatc ttcaggccgt ggcgtgcggc
     5941 gaaaacctcg agctgtggac gtcgcgccat ttgcccatcc tcggcgagga tttccaccag
     6001 cacgccggcg ggttcgcagc cggccagagc cgccaggtcg ctcgcggctt cggtgtgccc
     6061 cgcacgcgtg agcacaccgc ccggctgtgc ggcgagcggg aagatgtggc caggctggac
     6121 caggtcggcg ggtttggtgt ccgcgccgac ggcagtgcgg atcgtgtgcg cacggtcgtg
     6181 cgcggaaatg ccggtggtga cgccggccgc cgcctcgatc gagacggtga aattggtgtg
     6241 gtggcgcgag gcattcgcgc tgaccatcgg cggcaggcgc agttgcgcgc agcgctcgcg
     6301 cgtgagcgaa aggcagacca ggccgcgcgc gtgcgtgacc atgaaattga cgtcctcggg
     6361 ccgcacgcat tgcgcggcca tgatgaggtc gccttcgttc tcgcgatcct cgtcgtcgac
     6421 gatcacgacc atgcgtccgg cgcggatgtc ggcgacaagt tcggggatcg tggcgaaact
     6481 catgcggtct ctccggcacg caaccgttcc acgtagcgcg cgatcagatc gacttcgatg
     6541 ttcaccgcgt cgccaacgcg cttgccgcgg aacgccgtga cgtcgaccgt gtgcggaatc
     6601 aggttcacgc cgaatcgatt tccgcgcacg tcgttcacgg tcaggctgac gccgtcgatg
     6661 cacaccgagc ccttcgccgc gatgtagcgc gacagcggcg cgggtacctc gaacgtccag
     6721 cgctgcgaac gcgcgtcgtg ctcgatggcg acgacgcggc caatgccatc gacatgcccg
     6781 gacacgaggt gtccaccgag gcgatcggac aggcgcaatg caggctccag attcaccgca
     6841 tcgccgacat gcagcgcagc gagcgtggtc agcgccaacg tttccacgga aacatcgaac
     6901 gcgtggatac caccctcgcg cgcgaccacg gtcaggcaca cgccggaaac gcacaggctg
     6961 tcgccaaggt tcaattcgac gtccaacgcc gccgtgtcca caaacaggcg cgcatcgccg
     7021 ccgcgcgctt cgctgcgcgt gacgattccg gtagcctgga cgattccggt aaacatcgcg
     7081 tcattcccta agcagcaggc gccaatcttc gccgatccgg cgttcatcga ccacgcgcag
     7141 gcgccgaccc gcggcgattt cggcaatcgg cggcaagttc agcaacgcac gtgcgccatc
     7201 gccgagcaga atcggcgcga cgtacaacaa aagctcatcg acccgattcg cggcgaacag
     7261 ggcaccgcac agcgtcggcc cggcctcgac ctgcaattca ttaacgccgc gcgcggcgag
     7321 cagggccaaa gccgcatcaa ggtcgaatcc gccatctgct tgcgcggcca cggctacgcg
     7381 ttcgacgccg gcatagcgat cgtcgcacgg ttgtgcgcct tcggcatgca gaatcaaggt
     7441 cggcgtgctg ccgtcaagca gacgggcttg cgcgggcaag gcatccaggc gcgcatcgag
     7501 cacgacgcgc aacgcgggca cgaatgcctc gccgtcggca agtcgcaccg tcagccgcgg
     7561 atcgtcgaca cgcgccgtgc ccgcgccggt caggatcgca ctgctgcgcg cgcgccagcg
     7621 ctgcacgtcc gcgcgcgcgg cctcgctggt gatccattgc gattggccgt tcgcgagcgc
     7681 ggtacggccg tccaggctca tcgcgagttt gacccgcacc cacggtcggc cacgttccag
     7741 gcgagaaata aatccgcgat tgagttcacg tgccgccccg cgcagcaggc cggtttccac
     7801 ggcgatgccg gccgcgcgaa gtttcgcgat accgccgcca gcgactttcg gattcgggtc
     7861 ctcgcaggcg gcaacgacgc gcgcaacgcc cgccgcgacc aatgcatcgg cgcagggcgg
     7921 cgtcctgccg aaatgcgcgc agggctccag cgtgacgtag gctgtcgcac cgcgcgcgca
     7981 catccccgcc gcgcgcagcg caaagacctc ggcatgcggc tcgccggcgc gggcgtggaa
     8041 tccttcgccg aggatttcat cgtcgcgcgc gatcacgcaa cccacgcgcg gattcggctg
     8101 cgtggtgtac aagcctttct cggccagatg cagggcgcgc gccatgtgcg cgtggtcggc
     8161 agcggaaaac ccgttcatgg cgctttcttg cgctggcgca gatcgccgag caacggcaat
     8221 tgcaggcctt cgagttcggg caaatccttc tcgagcttct cgatttcctc gcggaacgcc
     8281 tgcacgtcct cgaagcggcg gtacaccgag gcgaagcgca cgtaggcaac ctggtcgagg
     8341 cgcttgagtt ccgtcatcac ccattcgccg agcaggcgcg agggcacttc gcgctcgttg
     8401 accgcgcgca actggcgcac gacttcgcgc accacgttgt cgacggcgtc gctggcgacc
     8461 gggcgcttct gcagagcgcg ctccacgccg gtgcgcaact tgtgttcgcc gaatgcctcg
     8521 cggcggccat cgctcttgac gatcgccggc agcttgattt ccatcgtctc gaacgtgttg
     8581 aaacgttcac cgcaatgcgt gcattcgcgg cgacgacgga tcgtcgtgcc gtcctcggat
     8641 tcgcgcgagt cgatgacgcg ggtgtcgacg tgctggcaga acggacagtg catctactct
     8701 cgcagactcg cgccagcggc gtttatcgcc gacaaacgat cagccataca ccgggaactt
     8761 gcggcactgc gcggtcacct tctcgcgcac cgcagcgatc accttgtcgt ccgccggtgc
     8821 gtcgagcacg tcgcagatcc agttcgcgag ttcgacgcaa tcggcttcgc cgtagccacg
     8881 cgtggtcacg gccggcgtgc cgatgcgcag gccggaggtc acgaagggct tctgcggatc
     8941 gttgggcacc gcgttcttgt tgacggtgat gtgcgcgcgg ccgagcgcgg cttcggcgtc
     9001 cttgccggtg acgcccttgg cgatcatgtc gaccagcatg aggtgattca tcgtgccgcc
     9061 ggaaacgatc ttgtagccgc gcgcgacgat cgtttttgcc atcgcctgcg cgttcttcac
     9121 cacgttgtgc tggtaggtct tgaacgcagg ttccagcgct tccttgaacg cgaccgcctt
     9181 ggcggcaatc acgtgcatga ggggtccgcc ctggatgccg ggaaacacga tggattgcaa
     9241 cttcttctcg atctcctcgg ccgccggccc catggcggaa tgtttggcga cgatcaggcc
     9301 gccgcgcgga ccgcgcaggg tcttgtgcgt ggtggaggtg accacgtgcg catgtggcaa
     9361 cggacttgga tacgcacctg cggcgatgag tcccgcgaca tgcgccatgt ccacgaacag
     9421 ataggcgccg accttgtccg cgatcgcgcg catgcgcgcc caatccacga cttgcgaata
     9481 ggcggaaaac cccgccacga ccatcttcgg cctgtgcacg acggcgagtt tctcgacttc
     9541 gccgtagtcg atgaacccgt ccgcgttcac gccatattgc acggcattga ggatcttgcc
     9601 ggagagattc actttcgcgc catgcgtgag atgcccgccg tgggcgagcg acatgcccag
     9661 gatcgtgtcg ccgggattca gcagggcgaa atacacggcc tgattggcct gcgagccgga
     9721 atgcggctgc acgttggcgt aatcgcaatc gaacaattgc ttgagacgct cgatggcaag
     9781 gcgttcggca acatccacga attcgcaacc gccgtagtag cgcttgccgg gatagccttc
     9841 agcgtacttg ttggtcagca cgctgccctg cgcttcgagc acgcgcgggc tggcgtagtt
     9901 ttccgaggcg atcagttcga catggtcttc ctggcgctgg cgctcggcgg cgatggccgc
     9961 agccagttca gggtcgtagc cttcgatttt catggtggcg gcgaacatcg gagcgtgtct
    10021 ccagcggggc gattgcgcat tttacctgcg c
//
LOCUS       DASRVO010000329         2807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_321965, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000329 DASRVO010000000
VERSION     DASRVO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321965"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFF05_04150"
     CDS             complement(<1..143)
                     /locus_tag="VFF05_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HZW51028.1"
                     /translation="MSTLFISDLHLDDARPRIVQAFENLLATQATEVQALYILGDLFE
                     SYIG"
     gene            complement(188..832)
                     /locus_tag="VFF05_04155"
     CDS             complement(188..832)
                     /locus_tag="VFF05_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZW51029.1"
                     /translation="MFKRLICAIALLVPAAAFAAAPAAKPAAKAAEPAKIDAPAPAPA
                     ANPKVLLHTSMGDITLELYPDKAPKTVDNFLQYVKEGFYDGTVFHRVINNFMIQGGGW
                     TKDLQRKHPHAPIRNEANNGLSNLNGTVAMARTADPNSAAAEFFINVVDNKRLDYVAD
                     ANGPVSWGYAVFGKVVSGMDVVDKIKAVETGPQGPFVSDVPKTPIVIEKASVVQ"
     gene            complement(920..2458)
                     /locus_tag="VFF05_04160"
     CDS             complement(920..2458)
                     /locus_tag="VFF05_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZW51030.1"
                     /translation="MNARIERKSDNAATRIREGELFAAADIGSNSFHLIVARYEHGEP
                     RVIDRLRENIRLAAGLKSDGGLDDVHREKALACLARFGQRLRGVPATRVRAVATNAVR
                     QMRVPELFLAPAESALGHPIEVVSGREEARLIYLGVAHGIPDAPGRRLVVDIGGGSTE
                     FIIGERLEPLKKESLQVGCVASTLRFFVDGKLTPKRWQQAQTDIAMELQQFAADYRAF
                     GWDEAIGSSGTVRAIDAVLREAGWSDAGITRAALERLRDAVVECGSVDKVKFPGLNED
                     RAGIFAGGVAILEATFAALDIQRMRVAETSMREGLLYDMIGRAEHRDPRGASIDALAQ
                     RYAVDHAHAVRVEAGALALFDQVCAPWQLGPTERDWLGWAARVHEIGLAIAHSQYHRH
                     GAYIVGHSDLAGFARHEQQVLAVLLRCQRRKPDVAAVRALPERLQLPVSRVTALLRLS
                     VLLQRSRESGVPPRAALHADGPALQLALPRAWLDSHPLTGADLEQERSYLRSLDIKLQ
                     IAAA"
     gene            complement(2455..>2807)
                     /locus_tag="VFF05_04165"
     CDS             complement(2455..>2807)
                     /locus_tag="VFF05_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HZW51031.1"
                     /translation="LRPGVKGLSENIRVRSIVGRFLEHSRVYWFANGGDAEIWCASAD
                     WMGRNLLRRIEVCFPILDAELARRVYAEALENYLADNTQAWLLDAGGRYRRAVPGADM
                     PHSAQATLLTRLCP"
BASE COUNT          461 a          886 c          979 g          481 t
ORIGIN      
        1 ccgatgtagc tttcgaacag gtcgccgagg atgtacagcg cctggacttc ggtggcctgc
       61 gtcgcgagca ggttctcgaa cgcctggacg atgcgcgggc gcgcatcgtc caggtgcagg
      121 tctgagatga aaagggtgga catgcgcgca tgccgacgcg catagcgcgc cggcaacggc
      181 aaaaaagtta ctgcaccacg ctggccttct cgatcacgat cggcgtcttc ggcacgtcgc
      241 tcacgaacgg tccctgcggg ccggtttcca cggccttgat cttgtcgacc acatccatgc
      301 cggagacgac cttgccgaat acggcgtagc cccacgacac cgggccattg gcatcggcga
      361 cgtagtccag gcgcttgttg tcgacgacgt tgatgaaaaa ctccgcagct gccgaattcg
      421 gatcggcggt gcgcgccatc gcgaccgtgc cgttcaagtt ggacaggccg ttgttggctt
      481 cgtttcggat cggcgcgtgc gggtgcttgc gctgcaaatc cttggtccag ccgccgccct
      541 ggatcatgaa attgttgatg acgcggtgga agacggtacc gtcgtagaaa ccttccttga
      601 cgtactgcag gaagttgtcg acggttttcg gtgccttgtc cggatacagc tccagcgtga
      661 tgtcgcccat gctggtgtgc agcagcacct tgggattggc ggcgggggca ggtgcgggcg
      721 catcgatctt ggccggttcg gcggccttcg cggccggttt cgcggcaggc gcggcggcga
      781 acgcggcggc gggaacaagc agggcgatgg cgcagatcag gcgtttgaac atggcaggct
      841 ccatggctgg aaaagaaatg ggctggaaaa cgggaaacga atgatacgca ctatgacgca
      901 gaagtgcgca aaattcgcgt taggccgcgg cgatttgcag cttgatatcc aacgatctca
      961 ggtagctgcg ttcctgctcc agatccgcac cggtcagtgg gtggctgtcc agccaggcgc
     1021 ggggcagggc gagttgcaac gccgggccat cggcgtgcaa ggccgcgcgc ggcggcacgc
     1081 ccgattcgcg cgagcgctgc agcagcacgg acaggcgcag cagggcggtg acgcgcgaaa
     1141 ccggcagttg caggcgttcg ggcagcgcac gcacggcggc cacatcgggc ttgcgccgct
     1201 gacagcgcag cagcacggcc agcacctgct gttcgtggcg ggcgaatccg gcgaggtccg
     1261 aatggccgac gatgtaggcg ccgtgcctgt ggtattggct gtgcgcgatg gccagcccga
     1321 tctcgtgcac gcgcgcggcc caacccagcc aatcgcgttc ggtcggcccg agttgccacg
     1381 gcgcgcagac ctgatcgaac agcgccagcg cgccggcctc cacgcgcacg gcgtgggcat
     1441 ggtcgacggc gtaacgctgc gccagcgcgt cgatgctcgc gccgcgcgga tcgcggtgtt
     1501 cggcgcggcc gatcatgtcg tagagcaggc cctcgcgcat cgaggtttcg gccacgcgca
     1561 tgcgctggat gtcgagcgcg gcgaaggtgg cttcgagaat cgctacgccg ccggcgaaga
     1621 tgccggcgcg atcctcgttg agtccgggaa atttcacctt gtccacgctg ccgcattcga
     1681 ccaccgcgtc gcgcaggcgt tcgagcgctg cgcgggtgat gccggcgtcg ctccaaccgg
     1741 cctcgcgcag cactgcatcg atcgcgcgca ccgtgccgga cgagccgatc gcctcgtccc
     1801 agccaaaggc gcggtagtcg gcggcgaatt gctgcagttc catcgcgatg tcggtctgcg
     1861 cctgctgcca acgcttgggc gtgagcttgc cgtcgacgaa aaagcgcagc gtgctggcca
     1921 cgcagcccac ttgcaggctt tccttcttca ggggttccag gcgttcgccg atgatgaatt
     1981 cggtgctgcc gccgccgatg tcgacgacaa ggcgccggcc cggcgcgtcg ggaatgccgt
     2041 gggcgacgcc gagatagatc aggcgcgctt cctcgcggcc ggaaaccacc tcgatggggt
     2101 ggccgagcgc ggattccgcc ggcgcaagga acagttccgg cacgcgcatc tggcgtaccg
     2161 cattggtggc gacggcgcgc acgcgggtcg ccggtacgcc gcgcaggcgc tggccgaagc
     2221 gggcgagaca ggccaatgcc ttttcgcgat ggacgtcgtc gaggccgcca tcggatttga
     2281 gtccggcggc gaggcggatg ttttcgcgca agcggtcgat cacgcgcggc tcgccgtgtt
     2341 cgtagcgtgc aacgatgaga tggaaactgt tcgagccgat gtccgcagcg gcgaacagtt
     2401 cgccttcgcg gatgcgcgtg gctgcgttgt cgctcttgcg ctcgatgcgt gcgttcatgg
     2461 gcacagtctc gtcagcaggg tggcctgcgc ggaatgcggc atgtcggcgc ccggcactgc
     2521 ccgccggtat cggccacccg cgtccagaag ccatgcttgc gtgttgtcgg ccaggtaatt
     2581 ttccaacgcc tcggcgtaca cgcgccgcgc gagttccgca tcgaggatcg gaaagcacac
     2641 ctcgatccgg cgcagcaggt tgcgccccat ccagtccgcg ctggcgcacc agatctccgc
     2701 atcgccgccg ttggcgaacc agtacacgcg gctgtgttcg agaaagcggc cgacgatgga
     2761 gcgcacgcgg atgttttccg aaaggccctt cacgccgggg cgcaacg
//
LOCUS       DASRVO010000330         3823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_192519, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000330 DASRVO010000000
VERSION     DASRVO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192519"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFF05_04170"
     CDS             complement(<1..871)
                     /locus_tag="VFF05_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008574528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51032.1"
                     /translation="MPRMMRFASAILAIFACTAQAGTITVTDDSNVAPGSSTCTLAQA
                     IAVANGTNGVIAAPIGSITTNTGTCAGEGPGPNTIVFGTGVAATQVFSAADNYWYGPN
                     ALPPIASDITIDGGTTGVVLQASHMGDPTPATVNAFRFFYISGGLEFQSGALTLHDVT
                     LIGGVAKGGDGGAGGGGAGMGGAIYNQGTLMLNAVTLASNTARGGKGGANGAGGGGMG
                     EDATNAGGGGFGGSLGGAYGGAGGAATGSGGGGGGGFLGSAPGAGTGDSSAGAGGGIG
                     GLGGAGFGDGGSGG"
     gene            complement(1010..2224)
                     /locus_tag="VFF05_04175"
     CDS             complement(1010..2224)
                     /locus_tag="VFF05_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HZW51033.1"
                     /translation="MNANHRPLPRMLAAAILMALPCAAAIAQSAPGDAAQSRPIIELR
                     YRYESVDDAAIAQNADANTVRLRLGYRWAFAPGWRLVASGDRVQALAGAHYNSTANGK
                     TQYPTVADPQSSEFHEAYVGYADGGLDGALGRQIVSFDNQRFFGNVGWRQNEQTFDAL
                     SLRYAFDGGGPLLRYAYLDRVQRVNGNANPNPLLRAWDLNGNLLNLSQATQVGSFTGY
                     AYLLENDAIATGSTKTFGARWNGAHASGNWKFGWTLEYAHQNDYANNPVAQSADYRLI
                     EPTLTWSGVTFKIGDEVMGGNGRYGFATPFATLHAFDGWADRFTTTPVDGIDDRYLGA
                     NGNIGKAAWTTVWHDFHADRGGRAYGTEADAMLTYPLAAHLTGLLKLADYRSDGFSTD
                     ETKLWASLEYRF"
     gene            2423..>3823
                     /gene="nirK"
                     /locus_tag="VFF05_04180"
     CDS             2423..>3823
                     /gene="nirK"
                     /locus_tag="VFF05_04180"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HZW51034.1"
                     /translation="MKTRLFAFGMAAVLLGSAGLALADDAGTSPSNYIYGDFGPPRGD
                     PIHAVLTSPPNVPAPTLRNYPAKVIVELEVIEKEMPISEGVTYTFWTFGGTVPGSFIR
                     VRQGDTVEFHLKNNPNNKMPHNIDLHGVTGPGGGAASSFTAPGHQSQFTFKALNQGIF
                     VYHCATAPVGMHIANGMYGLILIEPPEGLAPVDHEYYVMQGDFYTTGKYREKGHQPFD
                     MEKAIDEKPTYVLFNGHEGALTGDKALTAKVGETVRLFVGNGGPNLVSSFHVIGEIFD
                     TVRPEGGTVEQHNVQTTLVPSGGAAMVEFHTEVPGSYVLVDHSIFRAFNKGALAILKV
                     DGPENKTVYSGKEVDSVYLGDRAEPNLAAVTQATKAAAAGKLTKEEQIKAGRQLFTGT
                     CSVCHQANGEGLSGVFPPLAKSSLIAEDPKHPIRILLHGLTGPVKVNGKDYNSVMPPM
                     NQLNDDEIANILTFVLN"
BASE COUNT          703 a         1255 c         1203 g          662 t
ORIGIN      
        1 tgccgccact tccaccatcg ccgaagccag cgccacccaa tccgccgatg ccgccgccag
       61 cgccagcgct ggaatcgccg gtgccggcac cgggagccga gccgagaaac ccgccgccgc
      121 cgccgccgcc gctgcctgta gccgcaccgc ccgcgccgcc ataagcaccg cccaggctgc
      181 cgccaaagcc gccaccgcca gcgttggtgg catcttctcc catgccgccg ccgccggcac
      241 cattggcgcc gcctttgccg ccacgggcgg tattggacgc gagcgtaacc gcgttcaaca
      301 tcaacgtacc ttggttgtag atcgcaccac ccatacccgc gccgccgcca cccgcgccgc
      361 catcaccgcc cttggcgacg ccgccgatca acgtcacgtc atgcagggtc aatgcaccgg
      421 actgaaactc cagtccgccg gaaatataga agaaccgaaa cgcattgacg gtcgccggag
      481 tcggatcgcc catatgcgaa gcctgcagca cgacgccagt cgtaccgcca tcgatcgtga
      541 tgtcgctggc tatcggcggc agtgcattgg gaccgtacca gtagttgtcc gctgcggaaa
      601 agacctgcgt cgcagcaacc ccggtgccga acacgatcgt gtttggccca gggccttcgc
      661 ccgcgcaggt acccgtgttg gttgtgattg aaccgatcgg ggctgcgatg acgccgttcg
      721 tgccgttcgc taccgcgatc gcctgcgcca atgtgcaagt gctactcccc ggagcaacgt
      781 tgctgtcgtc cgtcacggtg atcgtgccgg cctgcgccgt gcacgcgaaa atggccagaa
      841 tcgctgaagc gaaacgcatc atgcgcggca tgagattccc ctattttggc gagcagcctc
      901 atcggaaggg taccacgtgc cctctcccgc tcgaggaggg ggttgctggc cgcggcacga
      961 ccggcgcggt cgacgcagcc cttccccctc accggccgta acgcatgcct cagaacctgt
     1021 attcgaggct ggcccacagc ttggtttcgt ccgtactgaa accatcgctg cgatagtcgg
     1081 cgagcttgag caagccggtc aggtgcgcgg ccagcggata ggtcaacatg gcgtctgctt
     1141 cggtgccgta ggcgcgtccg ccgcggtcgg cgtggaagtc gtgccacacg gtcgtccacg
     1201 ccgccttgcc gatattcccg ttcgcgccga gataccggtc gtcgatgccg tcgacgggcg
     1261 tggtcgtgaa gcggtcggcc cagccgtcga acgcgtgtag cgtcgcgaag ggcgtggcga
     1321 agccgtagcg tccgttgccg cccatgactt cgtcgccgat cttgaacgtg acgccgctcc
     1381 aggtcagcgt cggttcgatc agtcggtagt ccgcactctg cgcgaccgga ttgttggcgt
     1441 agtcgttctg atgcgcatac tccagcgtcc agccgaattt ccagttgcca cttgcgtgcg
     1501 cgccattcca gcgtgcgcca aaggtcttcg tcgacccggt ggcaatggcg tcgttttcga
     1561 gcaggtaagc gtagccggtg aagcttccca cctgcgtcgc ctggctcagg ttgagcaggt
     1621 tgccgttcaa atcccaggca cgcagcagag gattgggatt ggcgttgccg tttacgcgct
     1681 gcacgcgatc cagataggcg tagcgcagaa gtggacctcc gccgtcaaaa gcgtagcgca
     1741 gcgacagcgc atcgaaggtc tgctcgttct ggcgccagcc gacattgccg aagaagcgct
     1801 ggttgtcgaa tgacacgatc tggcggccga gcgcgccgtc gagtccgcca tccgcataac
     1861 caacgtaggc ctcgtggaat tcgctggatt gcggatcggc cacggtcggg tattgggtct
     1921 tgccgttcgc cgtgctgttg tagtgcgcgc cggccagggc ctgcacgcgg tcgccggaag
     1981 cgaccagccg ccaacccggc gcgaatgccc agcggtagcc caggcgcagg cgcacggtgt
     2041 tggcgtcggc attttgcgca atcgcggcgt catccaccga ctcgtagcga tagcgcaact
     2101 cgatgatggg tctgctctgt gccgcgtcgc ccggcgcgga ttgggcgatt gctgcggcgc
     2161 aaggcaaggc catcaaaatg gcggcagcga gcatgcgcgg aaggggacgg tggttggcgt
     2221 tcatggtcgt ctctttatgc ggcgcaacgc cgcgttggcg ccagtctcgc cacgcgcggc
     2281 gtttgcgcgc tgacctggat caagacgatg ttgcggcgca cgataaagtc ggtgcgtgct
     2341 tgacccgagt caattaactc ggacgcatgg cgttgttaga tgctgccatc ggccatccgg
     2401 tcattttctc tggggtttgc acatgaagac gcgtctgttc gctttcggaa tggcggccgt
     2461 gctgctcggc agcgccggcc tggcgctcgc cgacgatgcc ggcacatcgc caagcaacta
     2521 catctacggc gacttcggcc cgccgcgcgg cgatccgatc cacgccgtgc tgaccagccc
     2581 accgaacgtg cccgcgccga cgctgcgcaa ttatccggcc aaggtgatcg tggaactcga
     2641 ggtgatcgag aaggaaatgc cgatttccga aggcgtgacc tacacgttct ggaccttcgg
     2701 cggcaccgtg ccgggcagtt tcatccgcgt gcgccagggc gacaccgtgg agttccacct
     2761 gaagaacaat ccgaacaaca agatgccgca caacatcgac ctgcacggcg tcaccggtcc
     2821 gggcggcggc gcggcgtcga gcttcaccgc gccgggacat cagtcgcagt tcacgttcaa
     2881 ggccctcaac cagggcatct tcgtatacca ctgcgcgacc gcgccggtgg gcatgcacat
     2941 cgcgaacggc atgtacggac tgatcctgat cgaaccgccg gaaggcctgg ctccggtcga
     3001 tcacgagtat tacgtgatgc agggcgattt ctacacgacc ggcaaatacc gcgagaaggg
     3061 tcatcagccg ttcgacatgg aaaaagccat cgacgaaaaa ccgacctacg tgctgttcaa
     3121 cggtcacgaa ggggcgctga ccggcgacaa ggcgctgacc gcgaaggtcg gcgagaccgt
     3181 gcgcctgttc gttggcaacg gcggcccgaa cctggtgtcg agcttccacg tcatcggcga
     3241 gattttcgac accgtgcgcc cggaaggcgg cacggtcgag cagcacaacg tgcagaccac
     3301 gctggtgccg tccggcggcg cggcgatggt ggaattccac accgaagttc cgggcagcta
     3361 cgtactggtc gatcattcga tcttccgcgc cttcaacaag ggcgcgctgg cgatcctgaa
     3421 agtggacggg ccggaaaaca agacggtgta ctcgggcaag gaagtcgatt cggtgtatct
     3481 gggcgaccgc gccgagccga acctcgccgc ggtgacgcaa gcaacgaagg ccgccgccgc
     3541 cggcaagctg accaaggaag aacagatcaa ggccggtaga caattattca ccggcacctg
     3601 ttcggtctgc caccaggcga atggcgaggg cctgtctggc gtgttcccgc cgctcgcgaa
     3661 atcgagtctc atcgcggaag accccaagca tccgatccgc atcctgctgc acggtctgac
     3721 cggaccggtg aaggtcaacg gcaaggacta caactcagtg atgccgccga tgaaccagct
     3781 caacgacgac gagatcgcga acatcctcac cttcgtgctc aac
//
LOCUS       DASRVO010000331         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_113853, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000331 DASRVO010000000
VERSION     DASRVO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113853"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFF05_04185"
     CDS             complement(<1..382)
                     /locus_tag="VFF05_04185"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZW51035.1"
                     /translation="MKGVPVLVTGAGGFIGAHLVGRLVELGADVRALLHYGADGGFGN
                     ARFLAEDVRAAVDWVQGDLLDTECLARIAAGRSIVFHLGALIAIPHSYKAPRSFLEVN
                     AIGTMNVLEAARAGNAKVVHTSTSE"
     gene            complement(487..>1058)
                     /locus_tag="VFF05_04190"
     CDS             complement(487..>1058)
                     /locus_tag="VFF05_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51036.1"
                     /translation="TVGGRAVSLRVFGKDCPVDGIPPERLDFRGWQSQQAVIRALAET
                     CDLLYCPYPFAADMADVSRLSFPSKLATYFAAARPILFHGPADSSPASYLRAKQAGWI
                     CATRNPDAVAASLVRLGGDAALYRRLAIGAHAAFADFTLETMRAATLRFLGYRDEPAP
                     YSFTGESLRETGGFGDDPWPVPERCAGNS"
BASE COUNT          217 a          354 c          323 g          164 t
ORIGIN      
        1 cttcggacgt cgaagtatga acgaccttgg cattgccggc acgcgcggct tcgagcacat
       61 tcatcgtgcc gatcgcgttg acttcaagga aactgcgcgg cgccttgtag gaatgtggaa
      121 ttgcgatcaa agcgccaaga tggaacacga tgctgcgtcc ggccgcaatc cgtgccaggc
      181 actcggtgtc gagcagatcg ccctgtaccc aatccaccgc agcgcgtaca tcctcggcca
      241 ggaagcgcgc attgccgaac cctccgtccg cgccatagtg cagcagggcg cgaacgtccg
      301 cgcccagttc caccaggcgg ccgacgagat gcgcgccgat gaacccaccg gcaccggtga
      361 ccagaaccgg aacgcctttc atgccgtcga acccgacgct atcgcgccag atcggcacgg
      421 tgaaggcgga agtcttcgat tcaggccgca ggaaatgggc atgtcgttgg tctcaacgct
      481 cgtatatcaa gaattaccgg cgcatcgctc cggcaccggc cacggatcat ccccgaatcc
      541 acctgtctcg cgcaacgatt caccagtgaa ggaatacggc gcgggctcgt cccgatatcc
      601 cagaaaccgc agcgtggcgg cgcgcatcgt ttcgagcgtg aaatcggcga acgccgcgtg
      661 cgctccgatc gccagtcgcc ggtacagcgc cgcgtcgccg ccgaggcgaa ccaggcttgc
      721 cgccaccgca tcgggattcc gggtcgcgca tatccacccc gcctgtttcg ctcgcagata
      781 cgacgcaggc gacgaatcgg cggggccgtg aaacagtatc gggcgagcgg cggcgaaata
      841 cgtcgccagc ttgctcggga aactgagcct cgatacatcc gccatgtcgg cggcgaacgg
      901 atacgggcaa tacagcaaat cgcaggtttc ggcgagcgcg cggatcacgg cctgctggga
      961 ttgccagccg cgaaaatcca gccgttccgg aggaatgcca tcgaccgggc agtccttgcc
     1021 gaacactcgc agggaaactg cccgaccgcc gacggtcc
//
LOCUS       DASRVO010000332         1699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_192617, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000332 DASRVO010000000
VERSION     DASRVO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192617"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VFF05_04195"
     CDS             <1..741
                     /locus_tag="VFF05_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZW51037.1"
                     /translation="ATVADFRRRDIAAGGQGAPLAPAFHAAMLARDGIDRVVLNLGGI
                     ANITILDDEPLRGFDTGPASCLLDAWAQEQLGEPFDRNGAFAARGEVDEALLARLLDE
                     PYFAAAAPKSTGREVFHLDWLHPRLHASRATPADVQATLVELTAHSIADAVRAHAPNA
                     REVLVCGGGVHNPVLMRAIALALTPVAVASTATRGIDPDFVEAVAFAWLARERMLGLA
                     PENVCNVTGARGARVLGGIYFGSSYFGV"
     gene            complement(726..1019)
                     /locus_tag="VFF05_04200"
     CDS             complement(726..1019)
                     /locus_tag="VFF05_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51038.1"
                     /translation="MPDILVRNIDDVLAERIKAIARERGWSINEVIVHVLRHSLGLGG
                     EDVARREIHDVAELRGTWNPGESAAFRKALEAFEKVDGTPLFVDPKKTDQTPK"
     gene            complement(1141..>1699)
                     /gene="ampG"
                     /locus_tag="VFF05_04205"
     CDS             complement(1141..>1699)
                     /gene="ampG"
                     /locus_tag="VFF05_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="in Escherichia coli this protein is a permease
                     involved in peptidoglycan recycling; member of major
                     facilitator superfamily; MFS; inner membrane protein;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HZW51039.1"
                     /translation="MVLYKLCDAFALSLSTTFLLRVPQFTLTQLGAINKTFGLFAGLL
                     GALAGGWVVTRMRLFPALLVLGVLQALVNLGYIWLAYSGPDLWVLATVVGAENFFSGL
                     GSTAFVVLVTALCNVRYSATQYALLSSLAAVGRVFLGPLAAWLVPQVGWPMFFAITAL
                     SALPGLMLIIVLRRAIEQAELARPH"
BASE COUNT          321 a          562 c          517 g          299 t
ORIGIN      
        1 gccaccgtgg cggatttccg ccgtcgcgac attgccgccg gcggccaggg cgcaccgctc
       61 gcccccgcct tccatgctgc gatgctggcg cgcgatggca tcgatcgcgt ggtgctgaat
      121 cttggcggca tcgccaatat cacgatcctc gacgacgagc cactgcgcgg attcgatacc
      181 ggtccggcga gttgcctgct tgatgcctgg gcgcaggaac aactcggcga accgttcgat
      241 cgcaacggcg ccttcgccgc acgcggtgaa gtcgacgagg cattgctcgc gcgcctgctc
      301 gacgagccgt attttgcggc cgcggcgccg aaaagcacgg gccgcgaagt gtttcacctg
      361 gattggcttc acccgcgctt gcacgcatcg cgcgccacgc cggcggacgt gcaggcgacg
      421 ctggtcgaac tcaccgcgca cagcatcgcg gatgcggtac gcgcgcacgc tccgaacgcg
      481 cgtgaagtgc tggtctgcgg cggcggcgtg cacaatccgg tattgatgcg tgcgatagca
      541 ttggcactga cacccgtcgc cgtcgccagc acggccacac gcgggattga tccggatttc
      601 gtcgaagcgg tggcgttcgc ctggctcgcg cgtgagcgca tgctcggact tgcgccggaa
      661 aatgtctgca atgtcaccgg cgcgcgcgga gcacgcgttt tgggcgggat ctatttcggc
      721 tcgagttact tcggcgtctg atcggttttt ttcggatcga cgaacaacgg cgttccatcg
      781 accttctcga acgcctccag ggccttgcgg aatgcggccg attcgccggg attccaggtg
      841 ccgcgcaatt cggcaacatc gtggatttcg cggcgcgcca cgtcttcgcc gccaagtccc
      901 aggctgtggc gcagcacatg gacgatcacc tcgttgatcg accagccgcg ttcgcgcgcg
      961 atcgccttga tgcgctcggc gagcacgtcg tcgatgttgc gtacgagtat gtccggcacg
     1021 ctgccatttc cctttgctgt gcccctgcgg gcgggattct ggcagcaaat gccgcggatt
     1081 tcacgcacac caaccgcaaa caaacttgcc tgcgccctgg aatcctgcag accgggtgtg
     1141 tcaatgcggc cttgcgagct cggcctgttc gatcgccctg cgcagcacga ttatcagcat
     1201 caatcccggt agggccgata acgccgtgat ggcaaagaac atgggccacc cgacttgagg
     1261 aacgagccac gccgccagcg gccccaagaa cacccgcccc acagccgcca gcgacgacag
     1321 cagcgcgtat tgcgttgccg aatagcgaac attgcaaagt gccgtcacga gaaccacgaa
     1381 cgcggtactg cccagcccac tgaagaagtt ttcagcaccc acaacggtcg ccagtaccca
     1441 taagtccgga ccgctgtacg ccaaccagat gtagcccaaa ttcaccagcg cttgcagtac
     1501 gcccaatacc agcaacgcgg ggaaaagccg cattcgtgtc accacccagc cgccggccag
     1561 cgcaccgagc aggccggcga ataagccgaa ggtcttgttg atcgcaccga gttgggtgag
     1621 cgtgaactgc ggcacgcgca gcaggaaagt tgtcgatagc gatagcgcga atgcgtcaca
     1681 caatttatac agcaccatc
//
LOCUS       DASRVO010000333         2692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_322073, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000333 DASRVO010000000
VERSION     DASRVO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322073"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1197
                     /locus_tag="VFF05_04210"
     CDS             136..1197
                     /locus_tag="VFF05_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HZW51040.1"
                     /translation="MTKPSQALVWMLGFLGAVAVLAVLLATKLIANFQANPFFNGVIL
                     AVLVFGIFVNFRQVLLLSREVEWIEAFKRSPPDRPLPVKPRLLAPMARMFAGRERAKF
                     SLSTHSLRSVLDSVYLRLEESRDLSRYLVGLAIFLGLLGTFWGLLVTIRSVADIIGSL
                     GAGSDPAAMFNTLKENLKEPLSGMSTSFSTSLFGLASSLVLGFLDLQSGHAQNRFYNE
                     LEDWLSSMTRLSSGALHGDGEASVPAYVQALLEQTADGLERMQRALTESERERRGSGE
                     QFGELNTQLGRLADLLSRETREFRSLAETQEELKNALKAIAAAPNGSERLSDDLRGEL
                     RLLSRTIAAAVDGKRAAVQ"
     gene            1194..2279
                     /locus_tag="VFF05_04215"
     CDS             1194..2279
                     /locus_tag="VFF05_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HZW51041.1"
                     /translation="MNSLARRRRALDIWPGFVDALSSLILVMVFVLLIFAIGQFVMSQ
                     TIAGKNRALADLNAQIAQLAKTLSLAQDGNAALNAKVKELSASLGQTTGERDTLKTRL
                     DAATTQAAKLAADIVALSELKAQLEQQVASLGAELDTSKKNLVAATDLNTHNAAQIEL
                     LNRQIAAVRDQLSKLSAALDLANANVKDRDRKIADLGAQLNLALANKVNQLEKYRSEF
                     FGKLRAALGNRADIRVVGDRFVVPTDVLFDSGSADLNPAAQLQMAKLAQTLNAVAAEI
                     PSNLDWIVRIDGHTDKRPIHTAQFASNWELSTARAVAIVKYLVVQGIPANRLSANGFG
                     QFQPLDPADTPEAYAKNRRIEIQLTNR"
     gene            complement(2314..2553)
                     /locus_tag="VFF05_04220"
     CDS             complement(2314..2553)
                     /locus_tag="VFF05_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZW51042.1"
                     /translation="MKFGDLILSKREQRKLSVRQVAAVVGTTPSYLYELEQGKTVNIR
                     LPLAAKLSQVLKVPLTTLAAAALAEEKRLTKKQRK"
BASE COUNT          495 a          904 c          798 g          495 t
ORIGIN      
        1 ccgacgatcc gtggaatgcc acacccacag cgcgagccaa gcctcgccgt tgacagcatt
       61 ccgccacatt cgtgtggaac cattccagac gcaaccagtc caagttcggc cgcatcggcc
      121 gccaggaatc ttgtcatgac caaaccttcg caagccctcg tctggatgct cggctttctc
      181 ggcgctgtcg cggtactcgc cgtgttgctg gcgacgaagc tgatcgcgaa cttccaggcc
      241 aatccattct tcaacggcgt gatccttgcg gtgctcgtgt tcggcatctt cgtcaacttc
      301 cgccaggtgc tgctgctgtc gcgcgaagtc gaatggatcg aagcgttcaa gcgttcgccg
      361 ccggatcggc cgctgccggt caagccacgc ctgctcgcgc cgatggcgcg catgttcgcc
      421 gggcgcgagc gcgcgaaatt ctcgctgtcc acgcattcgc tgcgctcggt gctggacagc
      481 gtgtatctgc gtttggagga atcgcgcgac ctgtcgcgct acctcgtcgg cctcgccatc
      541 ttcctcggcc tgctgggcac gttctgggga ttgctggtca cgattcgatc ggttgccgac
      601 atcatcggtt cgctcggcgc gggcagcgat ccggccgcca tgttcaacac gctcaaggaa
      661 aacctcaagg aaccgctgtc gggcatgtcg acgagtttct ccacctcgct gttcggactc
      721 gcctcgtcgc tggtgctcgg ctttctcgac ctgcagtcgg gtcacgcgca aaatcgtttc
      781 tacaacgaac tcgaagactg gctttccagc atgacgcggc tgtcgtccgg cgccttgcac
      841 ggcgacggcg aggcgagcgt gccggcctac gtgcaggcgc tgctcgaaca aaccgccgat
      901 ggcttggaac gcatgcagcg cgcgctgacc gaatccgagc gcgagcggcg cggctccggc
      961 gagcaattcg gcgaattgaa cacccagctc ggccgcctcg ccgatctgct cagccgcgag
     1021 acgcgtgaat tccgcagcct cgccgagacc caggaagaac tgaagaatgc cctgaaagcg
     1081 attgccgccg cgccaaacgg cagcgaacgc ctgtccgacg acttgcgcgg cgagctgcgc
     1141 ctgctctcgc gcacgattgc cgctgccgtc gatggcaagc gcgcagccgt gcaatgaaca
     1201 gtctcgcgcg ccgccgtcgc gcgctcgaca tctggccagg attcgtcgac gcgttgtcgt
     1261 cgctgatcct ggtgatggtg ttcgtgctgc tgatcttcgc gatcggccag ttcgtgatgt
     1321 cgcaaaccat cgccggcaag aaccgcgcgc tggcggacct caacgcgcag atcgcgcagc
     1381 ttgccaagac cttgtcgctc gcgcaggacg gcaacgcggc gctcaacgcg aaggtaaagg
     1441 aattgtccgc ctcgctcggc cagacgaccg gcgaacgcga cacgctcaag acccggctcg
     1501 atgccgccac cacgcaggcg gccaagctcg ctgcggacat cgtcgcgctc agcgaactca
     1561 aggcgcaact cgaacaacag gtcgcgagcc tgggcgccga actcgacaca tcgaagaaga
     1621 atctcgtcgc cgcgaccgat ctcaatacgc acaacgccgc tcagatcgaa ctgctcaacc
     1681 gccagattgc cgcggtgcga gatcagctga gcaagctctc cgccgcgctc gatctcgcca
     1741 acgcgaacgt gaaggacagg gatcgcaaaa tcgccgacct cggcgcgcaa ctcaatcttg
     1801 cactcgccaa caaggtcaac cagctcgaaa agtatcgttc cgaattcttc ggcaagttgc
     1861 gcgcggcgct cggcaaccgc gccgacatcc gcgtcgtcgg cgaccgcttc gtggtgccga
     1921 ccgacgtgct gttcgattcc ggatcggccg acctgaatcc ggccgcgcag cttcaaatgg
     1981 cgaagctggc gcagacgctc aatgccgtcg cggcggagat tccgtccaat ctcgactgga
     2041 tcgtgcgcat cgacggccac accgacaagc gcccgatcca caccgcacaa ttcgcgtcga
     2101 actgggaatt gtcgacggcg cgcgcggtgg cgatcgtcaa atacctcgtc gtgcagggca
     2161 ttcccgcgaa ccggctgtcc gcgaacggct ttggccagtt ccagccgctg gatcccgccg
     2221 atacgccaga agcctatgcg aagaaccggc gcatcgagat tcagctgacg aatcgatagg
     2281 attgcgccgc gaaagcagct tcgccgttca agcttacttg cgctgttttt tcgtcaggcg
     2341 cttttcttcc gccagcgcgg cggcggcaag ggtcgtgagc ggcaccttga gcacctggct
     2401 cagcttggca gccagtggca ggcggatgtt caccgtcttg ccctgctcga gttcgtacag
     2461 gtaactcggc gtcgtgccga ccaccgcggc gacttgccgc acgctgagct tgcgttgttc
     2521 ccgcttggaa agaatcaaat caccgaattt cattggatgt cacctctgcc cgtatccccc
     2581 tgtcgattat ccgctagagc gagtcgcttg gctatcggaa ccgtcaaggt tcaagcccgg
     2641 cgcgccggtt cgcgctgact gaatatcacg ctgccgagga taactagacc gg
//
LOCUS       DASRVO010000334         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_270149, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000334 DASRVO010000000
VERSION     DASRVO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270149"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFF05_04225"
     CDS             complement(<1..315)
                     /locus_tag="VFF05_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZW51043.1"
                     /translation="MSFDTLGLAPVLLRALAEQGYAEPTPIQSAAIPAALSGRDLLAG
                     AQTGTGKTAAFALPLLQRLFVENTPPASAKRRPRALILAPTRELAAQVHESLRGYAKH
                     LRI"
     gene            639..>1556
                     /locus_tag="VFF05_04230"
     CDS             639..>1556
                     /locus_tag="VFF05_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZW51044.1"
                     /translation="MQFLSALRDLNSEQRHVVAASFLGWTLDAFDYFLLVFVIPELAR
                     EFGASFAEVFFSTTLTLMMRPFGALIFGWLADRFGRRPMLMLDVILFAVFEAASALAP
                     SLAVLLVVRALFGIAMGGEWGIGASLAMESIPAKSRGLVSGLLQQGYPVGYLVGALVY
                     WLAFDHIGWRGMFALGILPALLVLYIRRRVPESPVWVAARDGGAPRRGFLDAMRGHWR
                     LFAYLVVLMAFFNMFSHGSQDVYVTFLKAQRHFDTATVSKLTIVLNLGAIVGGLFFGA
                     WSERIGRRRAIVIAALLALPMVPLWAWGGS"
BASE COUNT          228 a          469 c          545 g          314 t
ORIGIN      
        1 aatgcgcaag tgtttcgcgt agccgcgcag gctctcgtgc acctgtgcag cgagttcgcg
       61 cgtgggcgcg agaatcagtg cgcgcggacg gcgtttcgcc gacgccggcg gggtgttctc
      121 gacgaacagg cgctgcaaca gcggcagggc gaaagcggcg gtcttgccgg tgccggtctg
      181 ggcgccggcg agcaggtcgc gcccggacag tgcggctgga atcgcggcgg attggatggg
      241 agtgggctcg gcgtaacctt gttcggcgag cgcgcgcaac aacacgggcg caagcccgag
      301 ggtatcaaac gacattgtgg aagctccaaa taggcaaacg ccagcggcgc ttgcgatgaa
      361 tcccggagcg ttcccgttga accgcgccgc aggcataaag cctagctgtc cgtcatggac
      421 aattcggatg ggcgtcgggc cgagtggtgc aggcttggca gtcgcggagt gcggtgccgg
      481 aggccggagg gaaaaacgga gggtcgacgc tgtttctagc cgatgtaggc agaaaacgac
      541 gcgtagtata catggctcca ggacggattg cacgtcggca ttgacgctgg cagcgccagc
      601 ccctagcctc gctgggcttg gttgtgaagg ggatgacgat gcagtttttg tccgcattgc
      661 gcgatttgaa cagcgagcag cgccacgtgg tggccgcaag tttcctcggc tggacgctcg
      721 acgcgttcga ctacttcctg ctcgtgttcg tgatccccga actcgcgcgc gaattcgggg
      781 ccagtttcgc cgaagtgttt ttttccacga cgctcacgct gatgatgcgg ccatttggag
      841 cgcttatttt cggctggctt gccgaccgct tcggccggcg cccgatgctg atgctcgacg
      901 tgatcctgtt cgccgtattc gaggccgcat ccgccctggc gccgtcgctg gcggttttgc
      961 tggtcgtgcg cgcgctgttc ggcattgcca tgggcggcga gtggggcatc ggcgcgtcgc
     1021 tggcgatgga atcgattccg gccaagtcgc gtggcctcgt ttccggcctg ctgcaacaag
     1081 gttatcccgt gggctatctg gtcggagcgc tggtctactg gctggccttc gaccatatcg
     1141 gctggcgcgg catgttcgcg ctcggcatcc tgccggcgct gctcgtactc tatatccgcc
     1201 gccgcgtgcc ggaatcgccg gtgtgggtgg cggcgcggga cggcggcgcg ccacgacgcg
     1261 gtttcctcga cgccatgcgc ggtcactggc gcctgttcgc ctacctcgtc gtgctgatgg
     1321 cgttcttcaa catgttcagc cacggctcgc aggacgtgta cgtcactttc ctcaaggcgc
     1381 agcggcattt cgacacggcc acggtgagca agttgaccat cgtgctcaac ctcggcgcca
     1441 tcgtcggcgg attgttcttc ggcgcctggt ccgaacgcat cggccggcgc cgcgccatcg
     1501 tgatagccgc gctgctggcg ctgccaatgg ttccgctgtg ggcatggggc ggctcg
//
LOCUS       DASRVO010000335         1109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_165110, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000335 DASRVO010000000
VERSION     DASRVO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165110"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFF05_04235"
     CDS             complement(<1..586)
                     /locus_tag="VFF05_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971642.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZW51045.1"
                     /translation="MNALPSLIQQTAELSESVTQPIPGSRKIHVAGSRADLRVPMREI
                     QLADTPSMFGTEKNAPFTTYDTSGPYTDPEYRVDLAAGLPALRARWIDERGDTERLAD
                     FTSPFTRRHASARELDDVRFPAIPKPRVAKSGTNVSQMHYARRGIVTPEMEFIAIREN
                     QKIEAIRETHLLKQHPGQSFGASIPKLITPEFVRA"
     regulatory      complement(628..726)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            822..>1109
                     /locus_tag="VFF05_04240"
     CDS             822..>1109
                     /locus_tag="VFF05_04240"
                     /inference="COORDINATES: protein motif:HMM:NF022893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3016 domain-containing protein"
                     /protein_id="HZW51046.1"
                     /translation="MKNILRLSFVPALLLLFVASAMAQAADASRIQVKWAPNDQLSEV
                     KQNSLHRGYMEPAQWQKALGDYLRRRAERLLPADQRLDVSIDDIKLAGDYEP"
BASE COUNT          203 a          341 c          363 g          202 t
ORIGIN      
        1 cggcgcgcac gaattccggc gtgataagtt tcggaatcga tgcgccaaac gactggcccg
       61 gatgctgctt caaaagatgc gtttcgcgga tcgcttcgat cttctggttc tcgcgaatcg
      121 caatgaactc catttccggc gtgacgatgc cgcgccgcgc gtagtgcatc tgcgacacgt
      181 tcgtgccgga cttggcgaca cgcggcttcg ggatcgccgg aaagcgcacg tcgtcgagct
      241 cgcgcgcgct ggcgtggcgg cgcgtgaacg gcgaggtgaa gtccgccaac cgctcggtat
      301 cgccacgctc gtcgatccaa cgtgcgcgca acgccggcag gccggccgcc agatccacgc
      361 gatattccgg atcggtgtac gggccggacg tgtcgtaggt cgtgaacggc gcgttcttct
      421 cggtgccgaa catcgacggc gtatcggcca gttgaatctc gcgcatcggt acgcgtagat
      481 cggcgcgcga tccagcgacg tggatcttgc gcgagccagg aatcggctgc gtcacggatt
      541 cggaaagttc ggcggtttgc tggataaggc tgggcaacgc gttcatcggt tcgtcctgca
      601 tgggtcgcgc gttggcgcgg tgggacgaag cgggtccgcg aagacttcct acgccggaat
      661 tgaccggatc aggtacgaag ggtcaagtct cagcgccggc aaaccggagc acccccgctt
      721 caagacgcca ttgaaacacg aagcggcggt catggcgagc gatgccgcga cgaaactctg
      781 cgcttcggcg tctgtcatca tgtgcgatca cggagacgtc catgaagaac atcctgcgtt
      841 tgagtttcgt cccggccctg ctgttgcttt ttgtcgcttc ggccatggcc caggcagccg
      901 acgcgtcccg gatccaggtg aaatgggcgc cgaacgacca gctcagcgaa gtgaagcaga
      961 actcgctcca tcgcggctac atggaaccgg cgcaatggca gaaggcgctt ggcgattacc
     1021 tgcgccggcg tgccgagcgc ctgctgccag ccgaccagcg gctcgacgtc agcatcgacg
     1081 acatcaagct ggctggcgac tacgaaccc
//
LOCUS       DASRVO010000336         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_36699, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000336 DASRVO010000000
VERSION     DASRVO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36699"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFF05_04245"
     CDS             complement(<1..700)
                     /locus_tag="VFF05_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HZW51047.1"
                     /translation="MSAQMQSERAPPLHGAPLALLTVAVAISTFMEVLDLTIVNVSVP
                     HIAGSLGVSAQEGTWAISSYSLASAVMQPLTGWIARQFGEVRTFCISVLLFVAMSMLC
                     GLATSMPMLVVARLLQGAGSGPMVALSMALLLNNYPNDKKGIALAVWAMTVVIAPIFG
                     PILGGWLTDNYSWPWIFYINVPLGVLAGLLCWEYVPEPSVTVRRHFGIDWLGLFFLAL
                     GIGSLQIMLERGQRE"
     gene            complement(702..1865)
                     /locus_tag="VFF05_04250"
     CDS             complement(702..1865)
                     /locus_tag="VFF05_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZW51048.1"
                     /translation="MSDSPTASNGNGNGKRRRLLLIIATVFVVAGLAWLALWWFVFSL
                     REVTDDAYVSGNQVILSSQVPGTVVEILADDTQRVDAGQVLVKLDPTDSDLALAKAQS
                     ALAQAVRQVRQQTQMAGQYDAAITAQRENLARAQADLKRREPLLAEKAVAPEEVAHAR
                     QAVVDAQAALDASTRQSAAAHALVDGTTIAENPLVQQARAAYREAWINDHRNAIVAPI
                     SGYVAQRSVQVGSRVQPGAPLLTIVPLHDLWVDANFKESQLTHIRIGQVAHVHADVYG
                     GDVTFDGRVEGLAAGTGGAFALLPAQNASGNWIKVVQRVPVRIALDPKQLEKNPLRVG
                     LSTEVTVDTHERGGAVLAQQPASKAIAQTSVYSADFAAADKAADAIVKANLAR"
BASE COUNT          331 a          666 c          690 g          335 t
ORIGIN      
        1 cctcgcgctg gccgcgttcc agcatgatct gcagcgagcc gatacccagt gccaggaaga
       61 agagccccag ccagtcgatc ccgaaatggc gccggaccgt gaccgaaggc tcggggacgt
      121 actcccaaca gagcagcccg gccagcaccc cgagcggcac gttgatgtag aagatccacg
      181 gccaggaata gttgtcggtc agccaaccgc caagaatcgg accgaagatg ggcgcaatca
      241 cgaccgtcat cgcccatacc gccagcgcaa tgcctttctt gtcgttcggg tagttgttga
      301 gcagcagcgc catcgacagc gccaccatcg ggccggagcc cgcgccctgc agcaggcgcg
      361 cgacgacaag catcggcatg ctggtggcga ggccgcacag catcgacatc gccacgaaca
      421 acagcaccga gatgcagaac gtgcgcactt cgccgaactg gcgcgcgatc cagccggtca
      481 gcggctgcat caccgcgctg gccagcgaat aggagctgat cgcccaagtg ccttcctgcg
      541 cgctcacgcc gaggctgccg gcgatgtgcg gcaccgagac gttgacgata gtgaggtcca
      601 gcacctccat gaacgtgctg atcgccaccg ccacggtgag cagggccagt ggcgcgccgt
      661 gcagcggcgg ggcgcgctcg ctttgcattt gagcgctcat gtcagcgtgc gagattggcc
      721 ttgacgatcg cgtcggcggc cttgtccgcc gcagcgaaat cggcgctgta gaccgacgtc
      781 tgcgcgatgg ccttcgacgc cggttgctgc gccagcaccg cgccgccgcg ctcgtgcgtg
      841 tcgaccgtca cttccgtgga caggcccacg cgcaacggat tcttttccag ttgcttcgga
      901 tcgagcgcga tgcgcacagg cacgcgctgc accaccttga tccagttgcc ggaggcgttt
      961 tgcgctggca gcagcgcgaa cgcaccgccg gtgccggcag cgagtccttc cacgcggccg
     1021 tcgaacgtga cgtcgccgcc gtagacgtcg gcatgcacgt gcgcgacctg gccgatgcgg
     1081 atgtgggtga gctggctttc cttgaagttt gcgtccaccc acaggtcgtg cagcggcacg
     1141 atggtgagca gcggcgcgcc cggctgcacg cggctgccga cctgcacgct gcgctgcgcg
     1201 acgtaaccgg aaatgggcgc gacgatggcg ttgcgatgat cgtttatcca cgcctcgcga
     1261 taggctgcac gggcctgctg cacgagcgga ttttcggcga tcgtggtgcc gtccacgagc
     1321 gcgtgcgcgg ccgcggactg gcgtgtcgag gcatcgagtg cggcctgcgc atcgaccacg
     1381 gcctgccgag catgcgcgac ttcttccggc gccacggcct tttcggcgag cagcggctcg
     1441 cggcgcttga ggtcggcttg cgcgcgcgcg aggttttcgc gctgcgcggt gatggcggcg
     1501 tcgtactggc cggccatctg cgtttgctgg cgcacctggc gcacggcctg cgccagcgcg
     1561 ctttgcgcct tggccagggc gagatcggaa tcggtgggat cgagcttgac cagcacctgg
     1621 cctgcatcca cgcgttgggt gtcgtcggcg aggatttcca ccaccgtgcc cggcacttgc
     1681 gaactcaata tgacctggtt gccgctgacg taggcgtcgt cggtgacttc gcgcaacgag
     1741 aacacgaacc accatagcgc cagccaggcg aggccggcga cgacaaatac ggtggcgatg
     1801 atgagcagca ggcggcggcg tttgccgttg ccatttccat tgcttgcggt tggggagtcg
     1861 ctcatggcga tggggctccg gaagaagagg tgggtggagg attcgcgccg aacccgccgc
     1921 ccagcgcgcg ggtcagggcg atttcggccg ccagtgccgc cgcgtcgagt tgcacgctgg
     1981 cgtcgcgttg gcgatccagc atttcaccgg catcgagcac cg
//
LOCUS       DASRVO010000337         1854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_113996, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000337 DASRVO010000000
VERSION     DASRVO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113996"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="VFF05_04255"
     CDS             <1..176
                     /locus_tag="VFF05_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZW51049.1"
                     /translation="DPAELFAVGGQRTVVEGAQAWNPVFDVTPHGLIDAIVTERGVIE
                     RPDRERMRAVFGA"
     gene            173..610
                     /locus_tag="VFF05_04260"
     CDS             173..610
                     /locus_tag="VFF05_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HZW51050.1"
                     /translation="MKSHLRRLAIFSLVGVVGFVVDAGVLQLLVLGLAWDRYSGRLIS
                     FLFAATATWLLNRRYTFRGTRKHALGVEWARYILAMSGGFACNFTAYSALVYVFNVDR
                     QWLILAVAAGSVAGLGVNYTASHYWVYRHQRRPAAGTSDSAGN"
     gene            766..>1854
                     /locus_tag="VFF05_04265"
     CDS             766..>1854
                     /locus_tag="VFF05_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZW51051.1"
                     /translation="MAELAKEVIRVNIEDEMRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFGMYEANNVWNRAYVKCARIVGDVMGKFHPHGDSSIYDALVRMAQDFSLRYPL
                     VDGQGNFGSVDGDNAAAMRYTECRLERLASDLLADIDKETVDFQPNYDEKEFEPTVLP
                     TRVPNLLVNGSAGIAVGMATNVPPHNLGEIVDATIAVIDNPEISFEELLRLVPGPDFP
                     TYGIINGAAGIIEAYKTGRGRIYVRAKAGIETDDNGRETIIVTELPYQVNKKRLIEAI
                     AELHKEKKIEGISELRDESDKDGLRIVFEVRKDTMADVLLNNLYQQTQMQVTFGINMV
                     ALVDGQPQLLTLKRILEAFVRHRREVVTR"
BASE COUNT          363 a          556 c          593 g          342 t
ORIGIN      
        1 gtgatcccgc cgaattgttc gccgtcggtg gacagcgcac cgtcgtcgaa ggtgcgcagg
       61 cctggaatcc ggtgttcgac gtgacgccgc atggtttgat cgacgccatc gtgaccgagc
      121 gcggcgtgat cgaacgcccg gaccgcgagc gcatgcgcgc cgtgttcggc gcatgaaatc
      181 gcacctgcgc cggttggcga tcttcagcct cgtcggcgtg gtcggtttcg tcgtcgatgc
      241 cggcgtattg caattgcttg tgctcggcct ggcctgggat cgctacagcg gacgtttgat
      301 ctcgttcctg ttcgcggcca cggcgacctg gctgctgaac cggcgctaca cgttccgcgg
      361 tacgcgcaag catgcgttgg gcgtggaatg ggcgcgctac atcctcgcca tgagcggcgg
      421 attcgcctgc aatttcaccg cgtattcggc attggtatac gttttcaatg tcgaccggca
      481 gtggctgatc ctggccgtcg ccgccggttc cgtggccgga ttgggtgtga attacacggc
      541 ctcgcattac tgggtttacc ggcaccagcg gcgacccgcg gccggcacta gcgacagcgc
      601 cggaaattga ccgctcgaaa cggctattcc ggtcagtaaa atcaaggctt tgcgaggctt
      661 cgccgagtgc cggattgtgg tagaatttca gggttgaaaa acacccttcc agggtccgtg
      721 caaggcgggc cttgaaaccc gcaaaaatcc agggaaaaaa cctcaatggc ggagctggcc
      781 aaagaagtca ttcgcgtcaa tatcgaagac gaaatgcggc agagttacct cgattacgcg
      841 atgagcgtga tcgtcggccg cgccctgccg gacgtgcgcg acgggctcaa accggtgcat
      901 cggcgcgtac tgttcggcat gtacgaggcg aacaacgtct ggaaccgcgc ctacgtgaaa
      961 tgcgcgcgca tcgtcggcga cgtgatgggt aaattccatc cgcacggcga ctcgtcgatc
     1021 tacgacgcgc tggtgcgcat ggcccaggat ttctcgctgc gctacccgct cgtcgacggg
     1081 cagggcaact tcggttcggt cgacggcgac aatgccgcgg cgatgcgcta caccgagtgt
     1141 cggctggaac gcctcgcgtc ggacctgctc gccgacatcg acaaggaaac cgtcgatttc
     1201 cagccgaact acgacgaaaa ggaattcgag ccgaccgtgc tgcccacgcg cgtgccgaac
     1261 ctgctcgtca acggttccgc cggcatcgcg gtcggcatgg cgacgaacgt gccgccgcac
     1321 aatctgggcg agatcgtcga tgcgacgatc gcggtgatcg acaatccgga aatttccttc
     1381 gaggaattgc tgcgcctggt gccggggccg gatttcccga cttacggcat catcaacggt
     1441 gcggcgggca tcatcgaggc gtacaagacc ggccgcggcc gcatctacgt gcgcgccaaa
     1501 gccggcatcg aaaccgacga caacggccgc gagacgatca tcgtcaccga gctgccgtac
     1561 caggtgaaca agaagcgact gatcgaggcg atcgccgagt tgcacaagga aaagaagatc
     1621 gagggcatct cggagctgcg cgacgaatcc gacaaggacg gcctgcgcat cgtgttcgag
     1681 gtgcgcaagg acaccatggc cgacgtgctg ctgaacaatc tgtaccaaca gacgcagatg
     1741 caggtcacgt ttggcatcaa catggttgcg ctggtcgatg gccagccgca actgcttacg
     1801 ctcaagcgga ttctcgaagc gttcgtgcgc catcgtcgtg aagtggtgac gcgg
//
LOCUS       DASRVO010000338         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_296829, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000338 DASRVO010000000
VERSION     DASRVO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296829"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1059)
                     /locus_tag="VFF05_04270"
     CDS             complement(<1..>1059)
                     /locus_tag="VFF05_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016904579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZW51052.1"
                     /translation="GARPGWVLDQTFDLFDRLGATNEQLDFPVVYASALHGYASLDPE
                     ARSGDMTPLYEAIMKHVPAPKVDPDGPFQMRISQLDYNSYVGLIGIGRIQRGKVRTNM
                     PVAVIDRDGKKRNGKVLQVLGFMGLERREVPEAEAGDIIAISGVEGLGISDTICALDA
                     PEQLPVMTVDEPTISMTFCVNNSPFAGNKDFSGGKFLTSRQVRDRLMRETLHNVALKV
                     EETDDADKFKVSGRGELHLSVLIETMRREGYELAVSRPEVIIKEIDGVKQEPYEQLVV
                     DMEEQFQGGVMEKLGARRGQLKNMEPDGKGRARLEYLIPARGLIGFQTEFRTLTAGTG
                     LLFHVFDHYGPLAEATPRR"
BASE COUNT          173 a          328 c          337 g          221 t
ORIGIN      
        1 gcggcgcggc gtcgcctcgg cgagcgggcc gtaatggtcg aagacgtgga acagcaagcc
       61 ggtgccggct gtgagcgtgc ggaattcagt ctggaagccg atcagtccgc gcgcgggaat
      121 gaggtattcc aggcgcgcgc ggcccttgcc gtcgggttcc atgttcttga gctgcccgcg
      181 gcgagcgccg agtttttcca ttacgccgcc ctggaactgt tcttccatgt ccacgaccag
      241 ttgttcgtac ggctcctgct tcacgccgtc gatttccttg atgatgactt ccgggcgcga
      301 cacggcgagt tcgtagcctt cgcggcgcat cgtctcgatc aacacggata gatgcagttc
      361 gccacgtccg gaaaccttga acttgtcggc gtcgtcggtt tcttccactt tcaatgcgac
      421 attgtgcagg gtctcgcgca tgagtcgatc gcgcacctgg cggctggtca ggaacttgcc
      481 gccggaaaaa tccttgttgc cggcgaatgg cgagttgttc acgcagaacg tcatgctgat
      541 cgtgggctcg tccacggtca tcaccggcag ttgttccggc gcatccaacg cgcagatcgt
      601 gtcggaaatg cccagcccct cgacgccgga aatcgctatg atgtcgccag cctcggcttc
      661 cggcacttcg cggcgttcca ggcccatgaa gccgagcacc tgcaaaacct tgccgttgcg
      721 cttcttgccg tcgcgatcga tcaccgccac cggcatgttg gtgcgcacct tgccgcgctg
      781 gatgcggcca atgccgatga gtcccacgta ggagttgtag tcgagctgcg agatgcgcat
      841 ctggaacggg ccgtccggat cgactttcgg tgccggcacg tgcttcatga tcgcttcgta
      901 aagcggcgtc atgtcgcccg agcgcgcctc cgggtcgagg cttgcgtagc cgtgcagcgc
      961 ggacgcgtaa acgaccggga aatcgagctg ttcgttggtt gcgccgaggc gatcgaacag
     1021 gtcgaaggtc tggtcaagca cccagcctgg gcgtgcgcc
//
LOCUS       DASRVO010000339         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_87920, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000339 DASRVO010000000
VERSION     DASRVO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87920"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1174
                     /locus_tag="VFF05_04275"
     CDS             <1..>1174
                     /locus_tag="VFF05_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW51053.1"
                     /translation="NEPIDIIRAMIIGEIVAVALRALAANKLRSILTMLGIVIGVAAV
                     IAMMALGTGARHAIQARIASLGTTRLTVNPTRVVRGGIETSDIHHITMDDVQFLKERA
                     RLIVAIQPQQDRDLQVEYHGANTNVQITGATPNFLQVQNYRIAVGRMFTDADGHGRRR
                     VAVIGATVLHDLGFSSAADAVGKEIRIRAVGFTVIGVLASKGEAAGGFFDPDDQVLIP
                     FNTGRFIVFDTPYLNDIHVLAPNEAEISQTMMELQRIMRRAHRLPAGAADDFRIHSQM
                     DFLSALSDTTKIFGLLLAAIASVSLLVGGVGIMNIMLVSVTERTREIGIRKALGATQR
                     HILLQFLIEAVTLCALGGMIGVAVGVGGATALKSFMKWNEQVVPATVFVAFLFASLV"
BASE COUNT          213 a          358 c          385 g          218 t
ORIGIN      
        1 tcaacgagcc gattgatatc atcagagcca tgatcattgg agagatcgtc gctgtggcgc
       61 tgcgcgcgct ggcggcaaac aaacttcgtt cgattctcac gatgctcggc atcgtgatcg
      121 gtgtagcggc ggtgatcgcg atgatggcgc tcggaacggg cgcccggcat gccatccagg
      181 cgcgcatcgc gtcgctcggg acgacgcggc tgacggtgaa tccgacgcgc gtcgtccgcg
      241 gcggcattga aacgagcgac atacaccaca tcacgatgga cgacgtccag ttcctcaagg
      301 aacgcgcccg actcatcgtc gccattcagc cgcagcagga tcgcgatctg caggtggagt
      361 atcacggcgc caacaccaac gtgcagatca cgggcgccac gccgaatttc ctgcaggtcc
      421 agaactaccg catcgcggtg gggcgcatgt tcaccgatgc ggacggccac ggccgtcgcc
      481 gcgtggcggt gatcggagcg acggtgctgc acgacctcgg attctccagc gccgccgacg
      541 cggtaggcaa ggaaatccgc attcgcgccg tgggcttcac ggtgatcggc gtgttggcgt
      601 cgaagggcga ggccgccggc ggcttcttcg acccggatga ccaggtgttg attcccttca
      661 acaccggccg cttcatcgtc ttcgacacgc cgtacctgaa tgacattcac gtactggcgc
      721 cgaacgaagc ggagatttcc caaaccatga tggagctgca gcgcatcatg cgccgagcgc
      781 accggctgcc ggccggcgca gccgacgact ttcgcattca cagccagatg gacttcctga
      841 gtgcgttgag cgacacgacg aaaatcttcg gcttgctgct cgcggccatt gcgagcgtga
      901 gtctgctcgt aggcggcgtg ggcatcatga acatcatgct cgtctccgtg accgagcgca
      961 cgcgcgaaat aggaattcgg aaggcgctgg gcgcgacgca gcgtcacatc ctgctgcagt
     1021 tcctgatcga ggcggtcacc ctgtgcgcat tgggtggcat gatcggcgtt gcggtcggcg
     1081 tcggcggggc gacggcgctc aagtctttca tgaagtggaa cgagcaggtg gtgccggcga
     1141 cggtgtttgt ggcgtttctg ttcgcgtcac tcgt
//
LOCUS       DASRVO010000340         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_218401, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000340 DASRVO010000000
VERSION     DASRVO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218401"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFF05_04280"
     CDS             <1..291
                     /locus_tag="VFF05_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51054.1"
                     /translation="PKMLVYVGTEKDAPGVSISGLLYGNPKQFLLQLYAALFIIAFNI
                     VATFIILKIISIFVSLRMDEATLRVGDDAVHGETAYNFGDGEDVPAMASSGD"
     gene            582..>1008
                     /locus_tag="VFF05_04285"
     CDS             582..>1008
                     /locus_tag="VFF05_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51055.1"
                     /translation="MRQQLRTGLWIAFGCLAAAAWQLGAPPAASAQQAPPAAAPPAAP
                     AAPASNAMSTPAMSGPLVANPNPFSTAPIGPLGPIYFNGAITGMGLFQTAPETYAGDH
                     SDQFDITNGLVSLQNTSGMFQFFVQAGVYSFPSLGYAYIH"
BASE COUNT          177 a          327 c          303 g          201 t
ORIGIN      
        1 cccaagatgc tcgtgtatgt cggcacggaa aaggacgcgc caggcgtcag catcagcggg
       61 ttactctacg gcaaccccaa gcagttcctg ctgcagctct atgccgcgct gttcatcatc
      121 gcattcaaca ttgtcgccac cttcatcatc ttgaagatca tctcgatctt cgtgtcgctg
      181 cggatggacg aggcgacgct gcgtgtcggc gacgatgcgg tgcatggcga gaccgcctac
      241 aatttcggcg atggcgagga cgttccggcg atggcgtcct caggcgacta ggcagaggcg
      301 aaacgtgatg gacgcggccg attcgctccg aaccggccgc gctcgccgca gccggcggcg
      361 tccgaccaca ccgcaggtcg tcgctggccg cactgttcct gagcaactga tgaattttag
      421 ggcgaattgt tgcgtcgatg ccaatatcgg caagaaattg tcgacgcaat gtcgccctcc
      481 gttttctatc gcctgcgagc gcaatacact tagtcttacg caatcttggg cagttcacgc
      541 ccggcggcga tcgagccgct gatgatcggg ggatttgcag aatgagacag cagctgagga
      601 ccggcctgtg gattgcattt ggctgccttg cggcggctgc gtggcaactc ggcgcacctc
      661 ccgccgcatc ggcgcaacaa gcaccgccgg ccgccgctcc gccggctgcc ccggccgcgc
      721 ccgcctcgaa cgcgatgtcg acgccggcga tgtcggggcc gctcgtcgct aatccgaacc
      781 cgttcagtac ggcgcccatc ggcccgctcg gcccgattta ttttaacggc gcgatcaccg
      841 gcatgggcct gttccagacc gcgccggaaa cctatgcggg cgaccattcc gaccagttcg
      901 acatcacgaa cggcctcgtt tcgctgcaga acacgtcggg catgttccaa ttcttcgtgc
      961 aggcgggcgt ctattcgttc ccgtcgctgg ggtacgccta tattcatg
//
LOCUS       DASRVO010000341         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_243877, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000341 DASRVO010000000
VERSION     DASRVO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243877"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1774)
                     /locus_tag="VFF05_04290"
     CDS             complement(137..1774)
                     /locus_tag="VFF05_04290"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HZW51056.1"
                     /translation="MIQDDVSAITRARLPDWLLRLLAAAVFAAVYSFVLLPVARFYWF
                     VPAGWRFAALALAPWRYWPWILGGELASRVWYEYVYDPPFAWQAPLPEFFFTLSGLVF
                     LFAGPLGGMLGSLYFRQRVRDIGAALEGVSGMSWLIVSCLIGALGETAGNLLSIHLNH
                     DVLQLSYSRFVVGKFVGDYIGVIGLAPALFALVFARKPRTWRLDALVVAAILAAYAAL
                     IRADLDEAVYGYLRLFVLIPTYWCAMRGGWRGAAVVLALSGFVVALAPSVHPLAPYHD
                     LFTQLLLAMFGSAALLLGSAMDAQRASSGELIRRNVHLEVANRDLERVGRELREVAER
                     NLTIEEEQRRRLARAIHDELGQNITAMHTRLKMAQTRINETQMEDVASSLYDILGQMR
                     RAVHALMDSLRPPVLDEFGLLRALDDGPLRDMVELAGMSYVFRLGGEPALIDALAENT
                     QIALWRIAQETSTNTVRHANAKVFEMRLRVGIRGGRVWALLDLRDDGVGIGAGESQRQ
                     RGGLQSIRDRVLALDGAMKVQRRGNITRLHLLLRQAL"
     gene            1907..2521
                     /locus_tag="VFF05_04295"
     CDS             1907..2521
                     /locus_tag="VFF05_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZW51057.1"
                     /translation="MIRTLLVDDHAIVRTGFRALIEREADMAIAGECASLAEADVVLE
                     GGAVDVLVLDISLRGGSGLAAVPDLRARHPALKIVMLSMHEAPVYVSEALAKGADGYI
                     TKTAAPEELIAGIHAVHSGERYVSADVANVRLRPAKLDLLSARERDVFVALARGRAPK
                     QVAAELGLAIKTVYVHRASVLVKLGARTDHDIYRIALENGLVET"
     gene            complement(2523..>2809)
                     /locus_tag="VFF05_04300"
     CDS             complement(2523..>2809)
                     /locus_tag="VFF05_04300"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017156796.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZW51058.1"
                     /translation="RGLTKLNANPERLAADLDANWEVLAEAVQTVMRRHGLPEPYEQL
                     KALTRGQGITRESLRTFIESLALPADAKKRLLALTPATYTGLAAKLAGGI"
BASE COUNT          498 a          908 c          957 g          446 t
ORIGIN      
        1 ccagaagcgc gttgccgcgc gtccggcggc cgatgccgcg ctgcgtgcgg aagggttgat
       61 caagcacaag gccgcagcgt aagacccgag tggaaagcgc cgggcggagc gatccgcccg
      121 gcgtcgcgtc cgtgcgtcac agcgcctgcc gcaacagcag gtgcaggcgc gtaatgttgc
      181 ctcgccgctg caccttcatc gccccgtcca gtgcaagcac gcggtcgcgg atgctctgca
      241 ggccgccgcg ttggcgctgg ctttcgccgg cgccgatgcc gacgccgtcg tcgcgcaggt
      301 cgagcagggc ccacacccgg ccgccgcgga tgccgacgcg caggcgcatc tcgaagactt
      361 tcgcgttggc gtgccgtacg gtattggtgc tggtttcctg cgcgatgcgc cacagcgcaa
      421 tctgggtgtt ctcggcgagt gcgtcgatca gtgccggttc gccgcctaaa cggaacacat
      481 aggacattcc ggccagttcc accatgtcgc gcagcggccc gtcgtcgagc gcgcgcaaca
      541 ggccgaattc gtccagcacg ggcgggcgca gcgaatccat cagggcatgc accgcacgcc
      601 gcatctggcc taggatgtcg tacagcgagg acgccacgtc ctccatttgc gtctcgttga
      661 tgcgcgtctg cgccatcttc aggcgcgtgt gcatggcggt gatgttctgg ccgagctcgt
      721 cgtggatcgc gcgcgcgagg cggcgacgtt gctcttcttc gatggtcagg ttgcgctcgg
      781 ccacctcgcg cagctcgcgt ccgacccgtt caaggtcgcg attggccacc tccaggtgga
      841 cattgcgccg gatcagttca ccgctgctcg cgcgttgcgc atccatggcc gaaccgagca
      901 gcagggcggc gctgccgaac atcgccagca gcagttgcgt gaacaggtcg tggtagggag
      961 ccagtggatg caccgaaggc gccagcgcaa ccacgaagcc gctcagggca aggaccacgg
     1021 cggcgccgcg ccagccgccg cgcattgcgc accaatacgt cggaatcagc acgaacaatc
     1081 gcagatagcc atagaccgcc tcgtcgagat cggcgcggat cagtgcggcg taggcggcga
     1141 ggatggcagc cacgaccagc gcatccaggc gccacgtccg cggcttgcgc gcgaaaacca
     1201 gcgcgaatag cgcgggcgcc agcccgatca caccgatgta gtcgccgacg aacttgccga
     1261 cgacgaagcg cgagtagctc aattgcagaa cgtcgtggtt gagatggatc gagagcaggt
     1321 tgcccgcggt ttccccaagc gcgccaatca ggcacgacac gatcagccag ctcatcccgg
     1381 atacgccttc gagcgcggcg ccgatgtcgc gcacgcgctg gcggaaatac agagaaccga
     1441 gcatgccgcc gagcggtccg gcaaagagga ataccagtcc cgacaatgtg aaaaaaaatt
     1501 cgggcagcgg agcctgccac gcgaacggcg ggtcgtagac gtattcgtac cacacccgcg
     1561 aggccagttc gccgccgagt atccacggcc agtagcgcca cggtgcgagc gccagcgcgg
     1621 cgaagcgcca acccgccggc acgaaccagt agaagcgcgc gaccggcaag agtacgaagc
     1681 tgtacaccgc cgcgaacacg gccgcggcta gcaggcgcag gagccagtcg gggagacggg
     1741 cgcgggtaat tgcggaaaca tcgtcctgaa tcattgcatt accctcgtgc ctcgaggacg
     1801 cgccaagtct tgcgctggta cgtcgcgagc ctaaccgcgt ggtgccccgg cggcgatggg
     1861 aaaatatcct agttcatgag cgtgcgcttg cggcacaatt ccggccatga tccgcaccct
     1921 gctcgtcgac gaccacgcca tcgtgcgcac gggattccgc gcgctgatcg agcgcgaagc
     1981 cgacatggcg atcgccggcg agtgcgcgag cctggccgaa gccgacgtcg tactggaagg
     2041 cggcgcggtg gacgtgctgg tgctggatat ttccctgcgc ggcggcagtg ggctggccgc
     2101 ggtgccggat ctgcgcgctc gccatccggc attgaagatc gtgatgctga gcatgcacga
     2161 ggcgccggtc tacgtgagcg aggccttggc caagggcgcg gacggctaca tcaccaagac
     2221 ggcggcgccg gaggaattga tcgccggtat ccatgccgtg cattccggcg agcgctatgt
     2281 gagcgcggat gtcgccaatg tgcggttgcg gccggcgaag ctcgatctgc tcagcgcacg
     2341 cgagcgcgac gtgtttgttg ccctggcccg cgggcgcgcg cccaagcagg ttgccgcgga
     2401 attgggtctg gcgatcaaga ccgtgtacgt gcatcgcgcc agcgtgctgg tcaaactcgg
     2461 cgcacgcacc gaccacgata tctaccgcat cgcgttggag aatggcttgg tggaaacctg
     2521 actcagattc cgccggcgag cttggccgcc aatccggtgt acgttgccgg tgtcagcgca
     2581 agcaagcgtt tcttcgcatc cgcgggcagc gcgagtgatt cgatgaacgt gcgcagcgat
     2641 tcgcgggtga tgccttgccc gcgcgtgagc gccttcaact gttcgtaggg ttcaggcagg
     2701 ccgtggcggc gcatcaccgt ctgcacggct tcggcgagga cttcccagtt cgcatcgagg
     2761 tccgcggcga gacgttccgg gttcgcgttg agtttggtga ggccgcgca
//
LOCUS       DASRVO010000342         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_10472, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000342 DASRVO010000000
VERSION     DASRVO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10472"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFF05_04305"
     CDS             <1..215
                     /locus_tag="VFF05_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HZW51059.1"
                     /translation="GIALLIGNVTVIQHGKGEFHGQRMRYNTNTGEMESGDASPSSRV
                     HMVMQPKSEQPKPAANADCTKAAATH"
     gene            215..934
                     /gene="lptB"
                     /locus_tag="VFF05_04310"
     CDS             215..934
                     /gene="lptB"
                     /locus_tag="VFF05_04310"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005913374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HZW51060.1"
                     /translation="MLAATGLQKSFRGRAVVRDFAFSLEQGEVVGLLGPNGAGKTTTF
                     YMVVGLIGADAGRISLDEHDITQMPMHARARLGVGYLPQEPSVFRRLTVAQNVLAILE
                     LREDLDAAGRRAELESLLDELQIAHIADSRGVSLSGGERRRVEITRALAAKPRFMLLD
                     EPFAGIDPISVVEIQRIIKQLRARGIGILITDHNVRETLGICDRAYIINEGRVLIAGT
                     PDLIIQDDNARKVYLGENFRL"
BASE COUNT          202 a          342 c          302 g          172 t
ORIGIN      
        1 ccggcattgc gctgctcatc ggcaacgtca ccgtgatcca gcacggcaag ggcgaattcc
       61 acggtcagcg catgcgctac aacaccaaca ccggcgagat ggaaagcggc gatgcttcgc
      121 catccagccg cgtacacatg gtgatgcaac ccaagagcga gcaacccaag ccggccgcca
      181 acgccgattg caccaaggcc gcggctacgc actgatgctg gccgccaccg gattgcaaaa
      241 gagctttcgc ggccgtgccg tcgtgcgcga tttcgcgttt tccctggaac aaggcgaggt
      301 cgtcggcctg ctcggcccga acggcgccgg caagacgacc acgttttaca tggtggtcgg
      361 cctgatcggt gccgacgccg gccgcatcag cctggatgag cacgacatca cgcaaatgcc
      421 gatgcatgcg cgcgcgcggc tcggcgtagg ctacctgccg caggaaccat ccgtgttccg
      481 gcgcctgacc gtggcacaga acgtgttggc gatcctggag ctgcgcgagg acctggacgc
      541 cgccgggcgt cgtgcggagc tcgaatccct gctcgacgaa ttgcagatcg cgcacatcgc
      601 cgacagccgc ggggtgagcc tgtccggtgg cgagcgccgg cgcgtggaga tcacccgcgc
      661 actggcagcc aagccgcgct tcatgctgct cgacgaaccc tttgccggca tcgatccgat
      721 ttcggtggtg gagatccagc gcatcatcaa acaactgcgc gcgcgcggca tcggcatcct
      781 gatcaccgat cacaacgtgc gcgaaaccct gggcatctgc gaccgcgcct acatcatcaa
      841 cgaaggccgc gtgctgattg ccggcacgcc cgacctcatc atccaggacg acaacgcccg
      901 caaggtgtat ctgggcgaaa atttccgtct ctaaatcccg gtagcatccg caacttattc
      961 gcagtacatt cgtctcaagt cggcatgctt tttgccgtag tcaactttca tgaagcac
//
LOCUS       DASRVO010000343         1939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_373821, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000343 DASRVO010000000
VERSION     DASRVO010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373821"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1159
                     /locus_tag="VFF05_04315"
     CDS             164..1159
                     /locus_tag="VFF05_04315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZW51061.1"
                     /translation="MILSYSLRLLFLCLASFFLLHALAGVAAWLAERPAIRLAEGASA
                     RSAARFLFWMRILPPGIALAFVAGVCVPSYLRYESNLAGEEVGFVCMALAVLGFLVWA
                     FALARGLRAAIHSLQFERLCRRTGSTMRLAGEPSQMLVVEAPHAFLVQSGVLRPRIVI
                     SQPLLAGLSGAELDAALRHERAHWAFRDNGKRLLFAFLPEILPVGQAYGAIERAWAKV
                     TERAADDLASASGPQEALLLAAALVRLARLRGDTGMSTPLARASSSLAGLADDDLRGR
                     IERLLAPGPAASVREPAYTRILWGAAGVLAAGALALLASPALLSSVHEVLERLVN"
     gene            complement(1190..>1939)
                     /locus_tag="VFF05_04320"
     CDS             complement(1190..>1939)
                     /locus_tag="VFF05_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51062.1"
                     /translation="ATMRLQKILNRGLAALAFILLIAPWAAAQQYRILHADYGWRDQR
                     VDVTQRLRELARDHDVFRMGNSTFGIDPAHGHVKTLRIFAQEPDGDRRVFEYREGDTV
                     DGSIFAGWRGGDWGDRGDRNRDRGDRDRRDRRDQGQYVILRAYYGVPGSNVDVTDRLR
                     DLARDNLIFRMGNSTFGIDPAHGYVKMLRIWAQAPDGDVRVFDYREGSTVDGRIFSGW
                     GRGDWGRREWRGDWNGERDRRRDRRDDRNDD"
BASE COUNT          265 a          668 c          635 g          371 t
ORIGIN      
        1 ccggcactct gatcgcgagc tttctggacg ctgccgagcc ttcgcgcgca ctgcgggtct
       61 ccagcctgct cgaagccatc ggcgagcacg atccggcgct gctcgatgaa ctggagaaga
      121 agatccgcct gcggcgccgg cagctcgaag agcgaggtgg aaaatgattc tttcctattc
      181 gctccggctg ctgtttctgt gcctcgcgtc gttctttctg ctgcatgcgc tggcgggcgt
      241 ggccgcgtgg ctcgcggaac gtcccgcgat tcggctggcg gagggcgcca gcgcccgttc
      301 ggccgcacgc tttctattct ggatgaggat tctgccgccg ggcattgcgc tcgcttttgt
      361 ggccggggtg tgcgtgccca gctatcttcg ttacgaaagc aacctggcgg gcgaggaagt
      421 gggcttcgtc tgtatggcgc tggctgttct cggttttctc gtgtgggcct tcgctctggc
      481 ccgcggtttg cgcgccgcga ttcactcttt gcaattcgaa cgcctttgcc ggcgcacggg
      541 cagcacgatg cgcctggcag gcgagccgtc gcagatgctg gtggtcgagg cgccgcatgc
      601 cttcctggtg cagtcgggcg tgctgcgccc gcgcatcgtc atctcgcagc cgcttctggc
      661 cggtctttcc ggcgcggagc tggatgccgc gctgcggcac gagcgcgccc actgggcctt
      721 tcgcgacaac ggcaagcgcc tgctgttcgc ttttctgccg gagattctgc cggtggggca
      781 ggcatacggg gcgatcgaac gagcctgggc gaaagttacg gaacgcgcgg cggacgactt
      841 ggcttccgcc agcggcccgc aggaagctct gttgctggcc gcggcgctgg tccgcctggc
      901 gcgcttgcgg ggcgacaccg gcatgtcgac gccgctcgcg cgcgcgtcgt cttcacttgc
      961 ggggcttgcg gacgatgacc tgcgcggacg catcgagcgc ctgctcgctc ccggcccagc
     1021 cgcttccgtt cgggagccgg cgtacacgcg aattctctgg ggcgctgcgg gcgtcctggc
     1081 agcgggcgcc ctcgcgctgt tagcctcgcc ggcgctgctg tcttcggtgc acgaagtcct
     1141 cgaacggctc gtgaactagc cgaagcgccg cgacacgctt ccggaagcgc taatcgtcgt
     1201 tgcggtcgtc gcgacgatcg cgacgccgat cccgttcgcc gttccagtcg ccgcgccact
     1261 cgcggcggcc ccagtcgccg cggccccagc cggagaagat ccggccgtcg accgtgctgc
     1321 cctcgcggta gtcgaacacg cgcacgtcgc cgtccggcgc ctgcgcccag atccgcaaca
     1381 tctttacata tccgtgcgcg ggatcgatcc cgaacgtgct gttgcccatg cgaaagatca
     1441 ggttgtcgcg cgccaaatcg cgcagacggt cggtcacatc cacgttgctt cccggaacac
     1501 cgtaatacgc ccgcagaatg acgtactgcc cctggtcgcg ccggtctcgc ctgtcccggt
     1561 ctcccctgtc gcggttacga tcgccccggt ctccccaatc accgccccgc catccggcga
     1621 agatcgagcc atcgacggtg tcgccttcgc ggtattcgaa gacgcgccgg tcgccatccg
     1681 gctcctgcgc aaaaatgcgc agcgttttca cgtgcccgtg cgcgggatcg attccaaacg
     1741 tgctgttgcc catgcggaac acatcgtggt cgcgagccag ctcgcgcagc cgttgggtga
     1801 catcgacccg ctggtcgcgc cagccataat ccgcgtgcag gatccggtac tgctgggccg
     1861 ccgcccacgg cgcgatcaac aggatgaaag ccaaagcggc caggccgcgg ttcaaaatct
     1921 tttgcagacg catcgtcgc
//
LOCUS       DASRVO010000344         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_243950, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000344 DASRVO010000000
VERSION     DASRVO010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243950"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="VFF05_04325"
     CDS             <1..1485
                     /locus_tag="VFF05_04325"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HZW51063.1"
                     /translation="RPHQANGRAAGDLMNGAFGILAYGAYLPRRRLQRKAIAAANSWF
                     NPGQRALAKGERAMANWDEDSITMAVEAARDCLTGLRPASIAQLILASTTHPYDDRQN
                     AGIAATALHLGSALRVLDVTGSLRAGTSALATALATGASALVAAGEHRLAKAASPQEM
                     HYGDGAAALLVGHGEPIARLVAEHSETIDFVHQYRGHDRPHDYAWEERWIRDEGYMKI
                     VPAALAQLFKTAGTSASSVTHFCMPCTLPRVAVAVAKRVGLAEAALRDNLAAVCGDTG
                     AAHPLLMLAHALQDAKPGDRILVAAFGQGCDALLFEVTHAIAKLPPRAGVRGALARRK
                     EESNYERFLAFNDHITLERGMRAETDHSTPLSTLYRNRDMVTGLIGGRCRDCGTLQFP
                     RGRYCVNPKCNALDSQDDQPFSDLSAKVMSYTADVLTYSPDPPAYYGMVQFDGGGRMM
                     ADFADVDDGKVEVGMPMRMMFRIKENDAARGFVRYFWKAAPASI"
     gene            1538..>2328
                     /locus_tag="VFF05_04330"
     CDS             1538..>2328
                     /locus_tag="VFF05_04330"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HZW51064.1"
                     /translation="MGCSKFGERWDRNAEDLMVEAFEEGLKDAGIGRDQIEAAWFGTA
                     IEEQHVGKSGVPLAMTLRLPYIPVTRVENYCATGTEAFRGAVYAVASGAVDIALALGV
                     EKLKDTGYGGLPQRGRGGLNNFFWANISAPGSFAQLAAAYGAKHRVDPKDLKRAMAHI
                     SVKSHDNGAKNPKAHLRNRITEDDVINAPMIADPLGLYDCCGVSDGSACAIVTTPDIA
                     RGLGKKDLVTVKALQLAVSNGQEVQFGEWDGSYFATTRVAAKRAY"
BASE COUNT          415 a          756 c          800 g          357 t
ORIGIN      
        1 cggccgcatc aagcgaatgg gcgcgcagca ggcgacctga tgaacggcgc gttcggcatt
       61 ctcgcgtatg gcgcctacct gccgcggcgc cgactgcaac gcaaggccat cgcggcggca
      121 aacagctggt tcaaccccgg ccagcgcgcg ctggccaagg gcgagcgcgc gatggcgaac
      181 tgggacgagg actcgatcac gatggcggtc gaggcggcgc gcgattgcct taccgggctt
      241 cgcccggcgt cgatcgccca gctgatcctc gcctcgacca cgcatcccta cgacgacagg
      301 cagaacgccg ggatcgcggc gaccgcgctg catctcggat ccgccctgcg cgtgctcgac
      361 gtcaccggca gcctgcgcgc cggcacttct gcgctggcga cggcgctcgc gacaggcgca
      421 agcgcgctgg tggcggccgg cgagcaccgc ctcgccaagg ccgcgagccc gcaggaaatg
      481 cactacggcg acggagccgc ggcactgctg gtcgggcacg gcgagccgat cgcgcggctg
      541 gtggcggagc acagcgagac gatcgacttc gtgcatcagt atcgcggtca cgaccgcccg
      601 cacgactatg catgggagga gcgctggatc cgggacgagg gctacatgaa gatcgtgccg
      661 gctgcgctgg cgcagctgtt caagactgcg ggcacctccg catcgtcggt gacgcacttc
      721 tgcatgccct gcacgctgcc gcgcgtggcg gtggcggtcg caaaacgcgt cggcctcgcg
      781 gaagccgcgc tgcgggacaa tctggccgcg gtctgcggcg ataccggtgc cgcgcatccg
      841 ttgctgatgc tggcgcatgc gctgcaggat gccaagcccg gcgaccgaat tctggtcgcc
      901 gcgttcggcc agggctgcga tgcgttgctg ttcgaggtta cccatgctat cgccaagctg
      961 ccgccgcggg ctggcgtgag gggcgcgctc gcgcgccgca aggaagagtc gaattatgaa
     1021 cgcttcctcg ccttcaacga tcacattacg ctcgaacgag gcatgcgcgc ggaaaccgat
     1081 cacagtacgc cgctgtctac cctgtaccgc aaccgcgaca tggtgaccgg gctgataggc
     1141 gggcgctgcc gagactgcgg aacgttgcag ttcccgcgcg ggcgttactg cgtcaacccg
     1201 aaatgcaacg cgctcgactc gcaggacgac cagccgttct cggacttgag cgcgaaggtg
     1261 atgtcataca ccgccgacgt gctgacctac agccccgacc cgccggcgta ctacggcatg
     1321 gtgcagttcg acggaggggg gcgcatgatg gccgatttcg ccgacgtgga cgacgggaag
     1381 gtcgaggtcg gcatgccgat gcgcatgatg ttccgcatca aggaaaacga cgccgcgcga
     1441 ggtttcgtgc gctatttctg gaaggctgcg ccggcgtcaa tttgacagga ggtccgatat
     1501 ggcaaccgga atccgcgaca aggtagcgat cctcggcatg ggatgctcga aattcggcga
     1561 acgttgggac cgcaacgccg aagacctgat ggtcgaggcg ttcgaagagg gattgaagga
     1621 cgccgggatc gggcgcgatc agatcgaagc ggcgtggttc ggcaccgcga tcgaggagca
     1681 gcacgtgggc aagtccggcg ttccgctggc gatgaccttg cgcctgccgt atatcccggt
     1741 cacccgggtc gagaattact gcgccaccgg aaccgaggct ttccgcggcg cggtctatgc
     1801 ggttgcctcg ggcgcggtcg atatcgcgct cgcgctcggc gtcgaaaagc tcaaggacac
     1861 cggctacggc gggctgccgc aacgcggccg cgggggcctc aacaacttct tctgggccaa
     1921 catatcggct cccggctcgt ttgctcagct cgcggcggcc tacggtgcca agcaccgcgt
     1981 cgacccgaag gacctgaaac gcgccatggc gcacatctcg gtcaagagcc acgacaacgg
     2041 cgccaagaat cccaaggcgc atctgaggaa ccgtatcacc gaggacgatg tgataaacgc
     2101 gccgatgatc gccgacccgc tcgggttata cgactgctgc ggcgtgtcgg acggctcggc
     2161 ctgcgcgatc gtcaccacgc ccgacatcgc gcgcggcctg ggcaagaagg atctggtcac
     2221 cgtcaaggcg ctgcagctcg ccgtgtccaa cggccaggag gtgcagttcg gggaatggga
     2281 tggctcgtat ttcgccacca cgcgagtggc ggccaagcgc gcctataa
//
LOCUS       DASRVO010000345         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_139844, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000345 DASRVO010000000
VERSION     DASRVO010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139844"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFF05_04335"
     CDS             complement(<1..324)
                     /locus_tag="VFF05_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW51065.1"
                     /translation="MHQATTAFEPERIALDGADGVPLAVEAHGDVDAPALVFAHGFGQ
                     TRHAWDASAATLARAGWRCLAMDARGHGDSGWRNDGRYDFSQFVDDLMRLARIPADKP
                     ILVGAS"
     gene            579..2354
                     /locus_tag="VFF05_04340"
     CDS             579..2354
                     /locus_tag="VFF05_04340"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HZW51066.1"
                     /translation="MSKLESLKRWVDDVVRLTQPEHVHWCDGSEAENDALIDLMLQRG
                     DLVELNPRTHPNCYLHRSNPSDVARVEHLTFICTRNREDAGPNNHWMDPAEAHAKIDA
                     LFAGCMQGRTLYVIPYCMGPIDSPLSRCGVEITDSPYVVANMRIMTRMGAPALARIER
                     EGGFVRGLHSTGELDPHKRFIMHFPDELSIKSIGSGYGGNALLGKKCHALRIASWQAR
                     TEGWLAEHMLIVGIQNPKGETHYVAAAFPSACGKTNLAMLIPPESYRQAGWKVWTVGD
                     DICWMTPGADGRLWAINPEAGFFGVAPGTSAKTNPNALAMLNHDTIFTNVAVTADDDP
                     WWEGLPDGVPVKDWQGRDYVPANGPAAHPNSRFTVSARQCPSWSPHAEDVQGVPISAI
                     VFGGRREHLVPLVFEARDWTHGVLVGAAMGSETTAAATGAVGVLRRDSMAMKPFCGYN
                     FADYFAHWLSLDKPGAKLPKIFHVNWFRKGEDGKFLWPGFGDNMRVLEWILGRSNGSE
                     GAEETPIGNLPRVEDLNTEGLNLAHAKLEALLHVDLESWSAEYESIGEYLASYGARMP
                     QALLDEHQRISTQLTGEEMEAVGTP"
     gene            2430..3005
                     /locus_tag="VFF05_04345"
     CDS             2430..3005
                     /locus_tag="VFF05_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZW51067.1"
                     /translation="MDGLALTADVPIAELPVRDADAALVRRAVGGEAAAFEQLYRRHA
                     GRVHGAILRLVGMDRARAEELTQDAFVQAWRKLGSFRFESAFATWLHRLGVNVALMDL
                     RIRRDEDAMGDDDLQAAAGGEVPFCAAERGDLERAVAALPTRARAVLVLHDVEGLKHE
                     EISAELGMAVGSSKAQLHRARGLLRRMLADR"
     gene            3046..3501
                     /locus_tag="VFF05_04350"
     CDS             3046..3501
                     /locus_tag="VFF05_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51068.1"
                     /translation="MNIDNEFAWRNELRKLGGDAQPQRDLWPRIAARIAVRPATQRWR
                     MGLGLAATLALAFGVATLAWRAQSASTLPIAATAPAHGATSMAADNAPLAWAVPANPA
                     LAAASRDLDGASMQLQQALEQQPQAVFLVGLLNRTNAQRMRLLRQAPSV"
BASE COUNT          650 a         1170 c         1174 g          608 t
ORIGIN      
        1 cgaggcgccg acaaggatgg gtttgtcggc gggaatccgc gcgagccgca tcaggtcgtc
       61 gacgaactgg gagaaatcgt atcggccgtc gttgcgccag ccgctatcgc cgtggccgcg
      121 tgcgtccatc gccaggcaac gccatccggc tctcgccagc gttgccgcgc tggcatccca
      181 ggcatgccgg gtctggccga atccgtgcgc gaaaaccagc gccggcgcat cgacatcgcc
      241 gtgcgcttca acggccagcg gcaccccatc cgcaccgtca agcgcgatgc gttcgggctc
      301 gaatgcggtt gtcgcttggt gcatgggggc ggctggcata catgcgagta tggaaatccg
      361 gattggagta atcaatcagg ttgcgtcgac acataacgag agcatgtcat gctgcaatgc
      421 agcgtattcc ttgcttcccg ctgtcgccgc cagccattac catgccgcga tgctggattc
      481 gaccatgatt cgcgcgttga cgtgcagcaa cgccggacgg cgcagatcgt ccgcattatt
      541 ttttcatttt cagccgcaag gctgccgtaa ggagaaccat gagcaagctg gaatcactca
      601 aacgctgggt cgacgatgtc gtgcgcctga cccaacccga gcatgtgcat tggtgtgacg
      661 gttccgaagc cgagaacgac gcgcttatcg acctgatgtt gcagcgcggc gacctggtcg
      721 aactcaatcc gcgcacgcat ccgaattgct acctgcaccg ttccaatcct tccgacgtcg
      781 cacgcgtcga gcacctcacc ttcatctgca cgaggaaccg cgaagacgcc ggcccgaaca
      841 accactggat ggacccggcc gaggcgcacg ccaagatcga cgcgctgttc gccggttgca
      901 tgcaagggcg cacgctgtac gtgatcccct actgcatggg accgatcgat tcgccgctgt
      961 cgcgttgcgg cgtggaaatc accgatagtc cttacgtcgt cgccaacatg cgcatcatga
     1021 cgcgcatggg tgcgcccgcg ctggcgcgca tcgagcgtga aggcggtttt gtcaggggcc
     1081 tgcattcgac cggcgaactc gatccacaca agcgcttcat catgcacttc cccgacgagc
     1141 tttcgatcaa gtcgatcggt tcgggctacg gcggcaacgc cttgctcggc aagaaatgcc
     1201 acgccctgcg catcgcctcc tggcaggcac gcacggaagg ctggctggcc gagcacatgc
     1261 tcattgtcgg catccagaat ccgaagggcg agacgcacta cgtcgccgcg gcattcccat
     1321 cggcctgcgg caagaccaac ctcgccatgc tgattccgcc ggaaagctat cgccaggccg
     1381 gctggaaggt ctggacggtc ggcgacgaca tctgctggat gacgccgggc gcggacggcc
     1441 gcctgtgggc gatcaatccc gaggccggat tcttcggcgt ggcgccgggc accagcgcga
     1501 agacgaatcc gaatgcgctg gccatgctca accacgacac catcttcacc aatgttgccg
     1561 tcaccgccga tgacgatccg tggtgggaag gcctgcccga cggcgtgccg gtaaaggact
     1621 ggcagggtcg cgattacgtt cccgcgaacg gaccggccgc gcatccgaat tcgcgcttca
     1681 ccgtgtccgc gcggcaatgt ccgagctggt cgccgcacgc ggaagacgtg cagggcgtgc
     1741 cgatttccgc catcgttttc ggcggccgcc gcgagcatct ggtaccgctg gtgttcgagg
     1801 cgcgcgactg gacgcacggc gtgctggtcg gcgcggcgat gggttcggaa accaccgctg
     1861 ccgcgaccgg cgcggtcggc gtgctgcgcc gcgactcgat ggcgatgaag ccgttctgcg
     1921 gctacaactt cgccgactat ttcgcgcact ggctgagcct ggacaagccc ggcgcgaagc
     1981 tgccgaagat tttccacgtc aactggttcc gcaagggcga ggacggcaag ttcctgtggc
     2041 cgggcttcgg cgacaacatg cgcgtgctgg aatggatcct cggtcgcagc aatggcagcg
     2101 aaggcgccga ggaaaccccg atcggcaacc tgccacgcgt cgaggatctg aataccgagg
     2161 gcctgaatct tgcgcacgcc aagctcgaag cgttgctgca tgtcgatctg gaaagctgga
     2221 gcgccgaata cgagagcatc ggcgagtatc tggcttccta tggcgcgcgc atgccgcagg
     2281 ccctgctgga tgaacatcag cgtatttcca cgcagttgac cggcgaggaa atggaagcgg
     2341 tcggaacccc atgaaaaacg cttcttccgc aggaggctgc gggagaagcg cttaaacccg
     2401 gcatgccgac gccgcatcca agctcacgta tggatggcct tgcattgact gccgatgtgc
     2461 cgattgccga actgcctgtc cgcgacgcgg atgcggcgct ggtgcgccgc gccgtgggcg
     2521 gcgaggcggc ggccttcgag caactctacc gccgccatgc cggtcgtgtg catggcgcga
     2581 tcctgcgcct ggtcggcatg gatcgcgcgc gcgccgagga actgacccag gatgccttcg
     2641 tgcaggcctg gcgcaagctc ggcagctttc gcttcgagtc cgcgttcgcc acatggttgc
     2701 accgcctcgg cgtcaacgtc gcgctgatgg atttgcgcat ccgccgcgac gaggacgcca
     2761 tgggggacga cgatttgcag gcggcggcgg gcggcgaggt accgttctgc gcggccgagc
     2821 gcggcgacct ggaacgcgcg gtggcggcat tgccgacgcg cgcgcgcgcg gtgctggtct
     2881 tgcacgacgt cgagggtttg aagcatgagg aaatttcggc cgaacttggc atggccgtgg
     2941 gttcgtccaa ggcacagttg catcgtgcgc gcggactgct gcgccggatg ctcgcggatc
     3001 gctgagagcg aaatccgcga aaagagagta gtgaggagat caagcatgaa catcgacaac
     3061 gaattcgcct ggcgcaacga actgcgcaaa ctcggcggcg acgcgcagcc gcagcgcgac
     3121 ctgtggccgc gaatcgccgc gcgcattgcc gtgcgtccgg caacgcagcg ctggcgcatg
     3181 ggactgggat tggcggcaac cctggctttg gccttcggcg tcgctacgct cgcctggcgc
     3241 gcgcaatccg caagcacgct tccgattgcc gcgacggcac cggctcacgg cgccacgagc
     3301 atggccgcgg acaacgcgcc gctggcatgg gccgttcccg ccaatcccgc gctcgccgcc
     3361 gcgtcgcgcg acctggacgg cgccagcatg caactgcagc aggcgctgga acaacaaccg
     3421 caggccgtat tcctggttgg cctgctcaac cgcaccaatg cccaacgcat gcgcctgctg
     3481 cggcaggcgc cgagtgtgtg acgaaccgac agaggccaga ccatgaaaat gaacattctc
     3541 cacattgccg catcgtgcgc attgcttgcc ggcaccgggt ccgcgttcgc cgcgccgcag
     3601 gc
//
LOCUS       DASRVO010000346         1135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_88037, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000346 DASRVO010000000
VERSION     DASRVO010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88037"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VFF05_04355"
     CDS             <1..289
                     /locus_tag="VFF05_04355"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZW51069.1"
                     /translation="TAADRHVRADLFVAMFPAKLRRALGIFGELLGLAFALLLLGTGV
                     AITWQTYSYGDVSTTSLRFPLWIYGAALPVGALLMVIAYAVRCINLFRKKS"
     gene            315..>1135
                     /locus_tag="VFF05_04360"
     CDS             315..>1135
                     /locus_tag="VFF05_04360"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZW51070.1"
                     /translation="MFLALLVIGAPIYLALGTTGALMFGVDGSLLSFAQKLIDELNSQ
                     TLLAVPYFVIAATLMERGGVAKALIGAAEAWVGHVRGGLGLVCVVSCTIFAAMCGSSV
                     ATAIAMGTILVPAIREHDYEAHFAAGIIGASGTLGILIPPSLAFVVFGVLADESIPRL
                     FLAGVIPGLMQAALFSGYIIWYSHRHNYARGETPHGAERMRRTVYAIPAFMVPVIVLG
                     GLYSGIVTLTESAALSAVVAVLLSLFVYRGMQFRTLLPVLAEAIRNAAAIMIIIAV"
BASE COUNT          148 a          375 c          367 g          245 t
ORIGIN      
        1 gaccgcggcc gaccgccacg tcagggccga cctgttcgtc gcgatgtttc ccgcgaagtt
       61 gcgccgcgcg ctcggcatat tcggcgaact gctcgggctc gcgttcgcgc tgctgcttct
      121 gggcaccggc gtggccatca cctggcagac ctattcgtac ggcgacgttt cgaccaccag
      181 tctgcgcttt cccttgtgga tctacggcgc ggcgctgccg gtgggtgcgt tgctgatggt
      241 gatcgcctac gccgtgcgct gcattaacct gttcagaaaa aagtcctagc gccttgaccg
      301 ccgttttcct cgtgttgttc ctggcgctgc tggtgatcgg cgcgccgatc tatctcgcgc
      361 tcggtaccac cggcgcgctg atgttcggcg tcgacggctc gctgctctcg ttcgcgcaga
      421 agctcatcga cgagctcaat tcacagacgc tgctcgctgt gccctatttc gtcatcgccg
      481 ccacgctgat ggagcgcggc ggcgtagcca aagccctgat cggcgcggcc gaggcatggg
      541 tggggcacgt gcgcggcggc ctcgggctgg tgtgcgtggt ctcctgcacc atattcgccg
      601 caatgtgcgg ctcatcggtg gcgacggcga tagccatggg caccatcctt gtgccagcca
      661 tacgcgagca cgactacgaa gcgcatttcg ccgccggcat cataggcgcc tcgggcacgc
      721 tcggcatctt gattccgccc agtctcgcct tcgtcgtgtt cggcgtgctc gccgacgagt
      781 ccatcccgcg gctgtttctc gccggcgtga tacccggcct gatgcaggcg gcgctgtttt
      841 cgggctacat catctggtac agccatcgcc acaattacgc gcgtggcgag actccgcacg
      901 gcgccgagcg catgcgccgc acggtgtatg cgattccggc gttcatggtg ccggtgatcg
      961 tgctcggcgg gctgtacagc ggcattgtca ccctgaccga atcggccgcg ttgtcggcgg
     1021 tggtggcggt cctgctgtcg ctgttcgtct accgcggcat gcagtttcgc acgctgctgc
     1081 cggtgctggc ggaggcgatc cgcaacgccg ctgcgatcat gattatcata gcggt
//
LOCUS       DASRVO010000347         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_243996, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000347 DASRVO010000000
VERSION     DASRVO010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243996"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VFF05_04365"
     CDS             complement(<1..179)
                     /locus_tag="VFF05_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZW51071.1"
                     /translation="MTRILGIDPGSQRTGIGIIDADAGGASRHVFHTALDLLDNDTFP
                     LRLKQIFDEVCALIA"
     gene            complement(218..868)
                     /locus_tag="VFF05_04370"
     CDS             complement(218..868)
                     /locus_tag="VFF05_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW51072.1"
                     /translation="MTSKSHAAGTDHVILLHGLWMRGFTLAALRHRLEAAGFSVELFD
                     YASVLRGPEVGVERLVASAQKSKSKKIHFVGHSLGGLVALQALQRAPEITRGRVVCLG
                     SPLRGSAVARSVAQFPGGSLVIGKSLDILTSGLERWEGKQAVGAVAGRLPIGFGFAFG
                     ALASPHDGTVSVAETELPGLTDHCIVPATHTGLLFSQDAAEQTIAFLRSARFARDA"
     gene            complement(877..>1180)
                     /locus_tag="VFF05_04375"
     CDS             complement(877..>1180)
                     /locus_tag="VFF05_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51073.1"
                     /translation="FGLAALAALGILGASLLGRRNIPFAPLALSALVVYLTAVHVALQ
                     AEPRYSVPYRPEEVLLAITALAVLGEWIVARIRKFPPDNAPWNSDSSRMPRRMLVG"
BASE COUNT          233 a          377 c          381 g          189 t
ORIGIN      
        1 tcggcgatga gcgcgcagac ttcgtcgaaa atctgtttca ggcgcaacgg aaaggtgtcg
       61 ttgtcgagaa gatccagcgc cgtgtggaag acgtgccgcg acgcaccgcc cgcatccgca
      121 tcgatgatgc cgatgccggt acgctgcgaa ccgggatcga tgccgagtat gcgggtcatc
      181 gtgcggattt tagcactgcg cgaaagccgg gccgcgctca ggcgtcgcgg gcaaagcgcg
      241 cgctgcgcag gaaggcgatg gtctgttcgg cggcatcctg cgagaacagc aggccggtgt
      301 gcgtggccgg cacgatgcaa tggtcggtca atcccggcaa ttcggtttcc gcaacgctga
      361 cggtgccgtc atgcggcgag gccagcgcgc cgaacgcaaa gccgaagccg atcggcaggc
      421 gtccggcgac cgcgccgacg gcctgcttgc cttcccagcg ttcgaggccg ctggtgagta
      481 tgtcgaggct cttgccgatc accagcgagc cgccggggaa ttgcgccacc gagcgcgcca
      541 ccgcgctgcc gcgcaacggc gaacccaggc agaccacgcg gccgcgggtg atctccggcg
      601 cgcgctgcag cgcctgcagc gccaccagtc cgccgagact gtgcccgaca aagtggattt
      661 ttttactttt gctcttttgc gcggaggcga cgaggcgctc cacgccgacc tcgggaccac
      721 gcaacacgct cgcatagtca aagagttcaa ccgaaaaccc cgccgcttcc aggcgatggc
      781 gcagcgcggc cagggtgaag ccgcgcatcc acaggccgtg caacaggatg acgtgatcgg
      841 tgccggctgc gtgcgatttg cttgtcattc tgactttcat ccgaccagca tgcggcgcgg
      901 cattctagag gaatccgaat tccacggtgc attgtcagga ggaaacttgc gaatccgcgc
      961 aacgatccac tcgcccagaa cggcgagcgc agtgatcgcg agtaacactt cctctggccg
     1021 atacggaacc gaatagcgcg gttcggcttg caaggcgaca tgcacggccg tcaggtacac
     1081 gaccaaggca ctcaatgcga gtggtgcgaa cgggatgttc ctcctgccca acaatgacgc
     1141 gccaagaatc cccaatgccg ccaatgcggc aaggccgaat
//
LOCUS       DASRVO010000348         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_244006, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000348 DASRVO010000000
VERSION     DASRVO010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244006"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFF05_04380"
     CDS             complement(<1..711)
                     /locus_tag="VFF05_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HZW51074.1"
                     /translation="MFDWINSESALTTWFSAQSVSSVIGLDTEFMRTDTFYARLALIQ
                     AEIGGRTAIIDAPALNGHAALAAHLADTHNVCVMHSASEDLEALSAIIPSGPATLFDT
                     QIAAAMAGLGSGLSYQKLVAALLGVDLPKGETRSDWLKRPLTPSQLEYAAQDVEHLPQ
                     IHALLSARLAELGRAEWLAEDCRRLVEKVCRSTPDVQPQRAFPRASDWPREQQALLRK
                     VLLWRETKARTLDKPRSWI"
     gene            793..>1031
                     /locus_tag="VFF05_04385"
     CDS             793..>1031
                     /locus_tag="VFF05_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HZW51075.1"
                     /translation="MPGTEVVSKQAVYGGAAGIALLAFALIYRFYPGSPNAPAAPAPP
                     VESATVPAMSLMPSAKTAAKSTPAQPAKTSTEPAAP"
BASE COUNT          183 a          340 c          318 g          190 t
ORIGIN      
        1 gatccacgag cgcggcttgt ccaatgtgcg tgccttggtt tcgcgccaga gcagcacttt
       61 ccgcagcaat gcctgttgtt cgcgcggcca atccgacgcg cgcggaaacg cgcgttgcgg
      121 ctgtacatcc ggtgtggaac ggcaaacctt ttccaccagc cgccggcaat cctcggccaa
      181 ccattccgcg cggccgagtt cggcgagcct tgcgctcagc agagcgtgga tttgcggcag
      241 gtgttcgaca tcctgcgcgg cgtactccag ctgcgacgga gtgagcggac gcttgagcca
      301 atccgaacgc gtttctccct tgggcagatc cacgccgagc agggcggcta cgagtttctg
      361 gtagctcagg cccgatccga gacccgccat tgccgcggcg atctgcgtat cgaacaaggt
      421 ggccggaccg gacggaatga tcgccgatag cgcttcgaga tcctcgctgg cgctgtgcat
      481 cacgcacacg ttgtgcgtgt cggccagatg cgcggcaagg gccgcatggc cattcaatgc
      541 gggcgcgtcg atgatggcgg tgcgcccgcc gatttctgcc tggatcagtg cgagcctggc
      601 gtaaaacgta tcggtgcgca tgaattcggt gtcgaggccg atgacgctcg aaaccgattg
      661 cgcggaaaac catgtggtga gtgcggactc gctgtttatc cagtcaaaca cgattcattc
      721 cttgttgcgg aacacgcgcg agatggccta aggtgcgctc atcgtcagca tcgcaccttc
      781 gcacatcccg caatgccagg cactgaagtc gtcagcaagc aagccgttta cggcggcgcc
      841 gccggcattg ccttgctcgc gttcgcgctg atctaccgat tctaccccgg cagccccaac
      901 gcacctgctg ctcctgcgcc gcccgtcgaa tccgcgacgg ttccagccat gtcgctgatg
      961 ccatcggcga agacggccgc caagtccaca cccgcgcaac cggcgaagac gtcgaccgag
     1021 ccggccgcgc c
//
LOCUS       DASRVO010000349         1037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_270500, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000349 DASRVO010000000
VERSION     DASRVO010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270500"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFF05_04390"
     CDS             <1..195
                     /locus_tag="VFF05_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51076.1"
                     /translation="GNCIVTGTLLEPLVAPATVRLVVPARAHDATQNPSAPNNHANHL
                     RAQTLAVLRNDMSGFPWYLP"
     gene            complement(270..>1037)
                     /locus_tag="VFF05_04395"
     CDS             complement(270..>1037)
                     /locus_tag="VFF05_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW51077.1"
                     /translation="IGNIPVAAAGGAQIPLKELARIDVANGASFIYRENNSRYIGVQF
                     SVEGRDLAGAVEDAMTQVARRVHLPPGYRLDWGGEYKEYTASRSQMNVIVPVTLFVIF
                     LLLFVLYGNVKFPLITVLGVLLSAPAGAILAQWLTGTPFSVSSGIGYIVLFGVSVLTA
                     VVYISYVNELRRSGMALDAAIREGAILRLRPIMMTALVAALGLLPAALASGVGTDSQR
                     PFALVIVAGMLTRLLISIYLMPALYALVAREGDRLEV"
BASE COUNT          215 a          348 c          313 g          161 t
ORIGIN      
        1 ggaaactgca tcgtcaccgg cacgttgcta gagcccttgg tcgccccggc aacggtcagg
       61 ttagtcgtac ctgccagagc gcacgacgcg acccagaacc ccagcgcgcc gaacaaccac
      121 gcaaaccact tgcgcgcgca aaccctcgcc gttctgcgaa atgacatgag cggattcccc
      181 tggtacctgc cgtgatacag cttatctgca tggcattcca cgttcaatct gctagcgatt
      241 gaatccgttg ttgccgcctg cagtggctac tacacctcca atcgatcccc ctcccgcgcc
      301 accagcgcat acagcgcggg catcaggtag atgctgatca gcaggcgcgt gagcatgccg
      361 gcgacgatga ccagcgcgaa cgggcgctgc gaatccgtgc cgacgccgga ggcaagcgct
      421 gcgggcagta atcccagcgc cgcgacgaga gcggtcatca tgatcggacg caggcgcagg
      481 atcgcgcctt cgcgtatcgc cgcatccaac gccatgccgc tgcggcgcaa ttcgttgacg
      541 taggaaatgt agaccactgc ggtcagcacc gacacgccga acaacacgat gtagccgatc
      601 ccggacgaca ccgagaacgg cgtgccggtg agccactgcg caaggatcgc cccggcgggc
      661 gcggacaaca gcacgccgag cacggtgatg agcggaaact tgacgttgcc gtagagcacg
      721 aacagcagca ggaagatcac gaacagcgtc accggcacga tcacgttcat ctgcgaacgc
      781 gacgcggtgt attccttgta ttcgccgccc cagtcgaggc gatagcccgg cggcaggtgc
      841 acgcggcgcg cgacctgcgt catcgcatct tccaccgcgc cggcaaggtc gcggccttcc
      901 accgagaatt gcacgccgat gtagcgcgaa ttgttttcgc ggtagatgaa cgaggcgcca
      961 ttggcgacgt cgatccgcgc aagctccttg agtgggatct gcgcgccgcc cgcggccgcc
     1021 accgggatat tgccgat
//
LOCUS       DASRVO010000350         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_140007, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000350 DASRVO010000000
VERSION     DASRVO010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140007"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..2121
                     /locus_tag="VFF05_04400"
     CDS             67..2121
                     /locus_tag="VFF05_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZW51078.1"
                     /translation="MFEGLRFAHWKAEIDAEGIAVLTFDRSGASVNAISRAVLDELAQ
                     IVERLSFEPPKGLVIRSGKANGFIAGADIHEFEGFEAQPGGVLGSIEYGQRVFQNLAR
                     LRCPTVAAIHGFCMGGGTELALACRYRVATRDPKTKLGFPEVLLGIIPGWGGTARLPR
                     LIGAPQALPVMLTGKALGAEAARGLGVVDALTSPELLLDAAKEQIRHPHARPFAQRLR
                     AWATNIWPVRQILAPIVRKQTAVKARPDQYPAPFALIEVWRRGGSSIKQRLKIEARAV
                     AKLAATPTCRNLIRVYFLQERLKGLGAGSDAKIQRVHVVGAGVMGGDIAAWCALRGFD
                     VTLQDREMKFIQPALDRAQDLFAKRLKTPDRMKPASDRLKADVDGKGIAEADLVIEAI
                     FENLEAKQDLYRKTEPSMKAGALLATNTSSIPLDELRTAVKQDGRFLGLHYFNPVAQM
                     PLVEIVRQDHLDPANEKHALAFCKAIDKLPVPVKGTPGFLVNRILMPYLLEAMRLFKE
                     GVPGPVLDKAAKKFGMPMGPIELADTVGLDVCASVGKEVGTFLGLEIPDGLDALIAAG
                     KKGKKTGEGLYKWENGKPVKPDVDPKYQAPADITERMILPMLNESVSCLHDAVVDDAD
                     LLDAGVIFGTGFAPFRGGPIQHIRSEGADKIKAQLAELAGKYGKRFEPKQGWDSSALK
                     PV"
     gene            complement(2118..3020)
                     /gene="folP"
                     /locus_tag="VFF05_04405"
     CDS             complement(2118..3020)
                     /gene="folP"
                     /locus_tag="VFF05_04405"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZW51079.1"
                     /translation="MFDLVRTLDCNGRELRLDRARVMGIVNVTPDSFSGDGRALADAA
                     IVHGMKLAEEGADLLDVGGESTRPGAEPVSVDDEIARVVPVIEALAKRTELPISVDTS
                     KPEVMRAAVAAGAGMINDVYALRREGALDAAAELKVPVVLMHMLGEPRTMQDDPHYDD
                     VVSDVRRFLAERIFACEMSGIDKKRIVADPGFGFGKTLEHNLALLANLDQLAALGVPL
                     MAGLSRKAMVGTLTGREKHADRAAGSAAAAMVAVQKGAAIVRVHDVAATRDALAVWEG
                     VARGIKPVKKEAKSFGSQRFGDDD"
BASE COUNT          580 a         1061 c          993 g          553 t
ORIGIN      
        1 catttgaccg cgggtgcggc ttgcgcggat tatagcgatc ccgcccgtcg cccgcttgag
       61 gccgccatgt tcgaaggtct gcgcttcgcc cactggaaag ccgaaatcga cgccgaaggc
      121 atcgccgtgc tcacgttcga ccgcagcggc gcttcggtca acgcgatcag ccgggccgtg
      181 ctcgacgaac tcgcgcaaat cgtcgaacgc ctgtcgttcg agccgccgaa gggcctggta
      241 attcgctccg gcaaggcgaa tggcttcatt gccggcgccg acatccacga attcgagggc
      301 ttcgaggccc agcccggcgg tgtgctcggc agcatcgagt acggccagcg cgtgttccag
      361 aacctggcgc gcctgcgctg cccgacggtc gccgcgatcc acggattctg catgggtggc
      421 ggtaccgaac tggcgctggc ctgccgctac cgcgtggcca cacgcgatcc caagaccaaa
      481 ctcggattcc cggaagtgct gctcggcatc atccccggct ggggcggcac cgcacgcctg
      541 ccgcgcctga tcggtgcacc gcaggccttg cccgtgatgc tcaccggcaa ggcgctcggc
      601 gcggaagccg cgcgcgggct tggcgtggtc gatgcgttga cttcgccgga actgctgctc
      661 gacgcagcaa aagaacagat tcgccatccg cacgcacggc cgttcgcaca gcgcctgcgc
      721 gcctgggcga cgaacatctg gccggtgcgg cagatcctcg cgccgatcgt gcgcaaacaa
      781 accgcggtca aggcgcgtcc ggaccaatat ccggcgccgt tcgccctgat cgaagtctgg
      841 cgccgcggcg gatcgagcat caagcagcgc ctgaaaatcg aagcgcgtgc ggtggccaag
      901 cttgcggcga cgccgacctg ccgcaatctg attcgcgttt attttttgca agagcgcctg
      961 aaaggtcttg gcgccggcag cgacgccaaa atccagcgcg tgcacgtcgt cggcgccggc
     1021 gtgatgggcg gcgacatagc ggcctggtgc gcgctgcgcg ggtttgacgt gacgcttcag
     1081 gatcgcgaga tgaagttcat ccagcctgcg ctcgatcgcg cgcaggacct attcgccaag
     1141 cgcctgaaaa cgcccgatcg catgaaaccc gcgtcggatc gcctcaaggc cgacgtcgac
     1201 ggcaaaggca tcgccgaagc ggatctcgtc atcgaggcga tcttcgagaa cctcgaagcc
     1261 aagcaggatt tgtatcgcaa gaccgaacct tcgatgaagg ccggcgcatt gctggcgacc
     1321 aacacatcga gcatcccgct cgacgaactg cgcacggcgg tgaaacagga cggccgtttc
     1381 cttggcctgc attatttcaa tccggttgcg cagatgccgc tggtggaaat cgtgcgccag
     1441 gatcacctgg atcccgccaa cgagaagcac gcgctcgcct tctgcaaggc catcgacaag
     1501 ctgcccgtgc cggtcaaggg cacgccaggc ttcctcgtca atcgtatttt gatgccctat
     1561 ctgctcgaag ccatgcgcct gttcaaggaa ggcgtgcccg gtcccgtgct cgacaaggcg
     1621 gcgaagaaat tcggcatgcc gatgggcccg attgaactcg ccgacaccgt gggcttggat
     1681 gtgtgcgcct cggtcggcaa ggaagtcggc acgttcctcg gcctggaaat tcccgacggt
     1741 ctcgatgccc tcatcgcggc cggcaagaaa ggcaagaaga ccggcgaagg tttgtacaaa
     1801 tgggagaacg gcaagccggt caaacccgat gtcgatccca agtatcaggc tcccgccgac
     1861 atcaccgagc gcatgatcct gccgatgctc aacgagtcgg tttcctgcct gcacgatgct
     1921 gtggtcgacg acgccgacct gctcgatgcc ggcgtcatct tcggcaccgg cttcgccccg
     1981 ttccgcggcg gcccgatcca gcacatccgc agcgagggcg cggacaagat caaggcgcaa
     2041 ctcgcagaac tggccggcaa atacggcaag cgtttcgagc cgaaacaagg atgggacagc
     2101 tcggcgttga aaccggttta atcgtcatcg ccgaaccgct gtgagccaaa cgacttcgct
     2161 tccttcttca cgggtttgat tccgcgcgcc acgccttccc ataccgccag cgcatcgcgc
     2221 gtcgcggcga cgtcgtgcac gcgtacgatc gcggcgcctt tctgcacggc aaccatcgcc
     2281 gcggctgcgg aacctgcggc gcgatccgca tgtttttcgc ggcccgtgag cgtgccaacc
     2341 atcgccttgc gcgacaatcc cgccatcagc ggcacgccca acgctgcaag ttgatccagg
     2401 ttggcaagca gcgcgagatt gtgttcgagc gtcttgccga aaccaaatcc ggggtcggcg
     2461 acgatgcgtt tcttgtcgat cccggacatc tcgcacgcaa aaatccgctc ggccaggaat
     2521 cggcgcacgt cggaaacgac gtcgtcgtag tgcgggtcgt cttgcatcgt gcgcggctcg
     2581 ccgagcatat gcatgagcac gaccggcact ttaagctcag ccgccgcatc cagcgcacct
     2641 tcgcggcgca gcgcgtacac gtcgttgatc atgcccgcgc cggcggcaac cgccgcgcgc
     2701 atgacttcgg gtttcgaggt gtcgacggaa atcggcaatt cggtgcgttt ggccagcgct
     2761 tcgatcaccg gcacgacacg cgcgatttca tcgtctactg atacgggttc ggcgcccggc
     2821 cgcgtggatt cgccgccgac atcgagcaag tcggcaccct cttccgccaa tttcatcccg
     2881 tgcacaatgg ccgcatcggc cagcgcgcgc ccgtcgccgg aaaacgaatc cggcgtgaca
     2941 ttgacgatgc ccatgacgcg cgcgcggtcg aggcgcagtt cgcggccgtt gcagtcgagg
     3001 gtgcggacta gatcgaacat cgctgtactc taacaaaacg aaggccgccc tcgggcggcc
     3061 ttcaattcga gtatggcagg taccgctcaa tgttgtgcag cgggcccgcc gagtggcggc
     3121 gaggccggtt tgccgacgcc cggcttgccg ccgtcgccgc cggtgccgga tttctgccag
     3181 tctttcg
//
LOCUS       DASRVO010000351         3701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_165492, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000351 DASRVO010000000
VERSION     DASRVO010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165492"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFF05_04410"
     CDS             <1..1126
                     /locus_tag="VFF05_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZW51080.1"
                     /translation="GLILAWRQRALLRADAGVRLAVILFACYWLAALISAPGAVAPEK
                     SWSTVAYLLRFLPFALFVCLALRRGAWWPLLTDACAAIVVLWLLDAWVQILTGWSLAG
                     AETADRLSGIFGAGNLKLGPVLAVLSPFVFTAARRRWGWRGLAIAFVFQLMPILFAGS
                     RAAWIMYGLVALAFVWMHTRAPLRFVAWSLAAACAALLGIAVAWQHSGRFDMRFDRTL
                     LALQGSQQALDAASAGRLRIWRTALAMSAAHPVAGVGVRGFRFAYPQYAQPGDAFVDA
                     QTDEGAFHAHQIVLEVLSETGSIGLLLWLTGAFCAIRAWCRAEAPARERALAPVLALG
                     TMTFPLNSHLAFYGTSAWGLLFWWLLALYCAALGSDEAPL"
     gene            1215..1985
                     /locus_tag="VFF05_04415"
     CDS             1215..1985
                     /locus_tag="VFF05_04415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW51081.1"
                     /translation="MALPISIFIIAFNEAARIGATLSSVRELSDDVIVVDSGSTDGTQ
                     DIAASFGARVVHNPWPGYGAQKRFGEDQCRHPWRLNLDADEVASLDLIAEIHSLFAHS
                     EPAAGAYSVRIVDVIPGDSKPRAFAYAHKKVRLNHKNAGRFSPSPVHDAVALHPAVKV
                     MRLRGAVHHFSMRTLGEQLHKFNAYTDALVLDLRRRNKSIPTWRVFVEFPMAFCKAYF
                     MRRHCLRGTYGFLTAMNYAYFRHLRVAKFHEARRNTHE"
     gene            complement(2016..3383)
                     /locus_tag="VFF05_04420"
     CDS             complement(2016..3383)
                     /locus_tag="VFF05_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HZW51082.1"
                     /translation="MKLALMLLCLSLSASADAASADDYAYAWPLQTPGDSSAWQVDLT
                     PEVYAAVRSADLRDVEVVNAAGESVPMALRAVQFSSAATSDVELPAFGLPPLAGMSPN
                     DDNLHLRIKRDVNGRLRQLDVDNDRRNAAQTAGHIILDARGLKDGAIDSLWLSWDEQS
                     NAVTAQFSVSGSDDLQQWRVLNANASVLAMQQNGNTLSRRQIALNGAHAKFLQLRRLD
                     TGAPLPSLHVRAHVLAHSTLIQPARVWVDAQPLPAQAGEGPASGTSSFRYRLPAPLAV
                     AALKLELATDNSLAHVRVSSGAPHVLHADFTAFRLRQDGTVLANDEIEIAPTPRTQDW
                     VVESTTPLDHVPTLRVAFRPDRFVFLTQGNGPYRLVAGSVRARRGDYPVDAALAQLRA
                     KFGNDWQPPLATLGARATLLGVTAYTPAPAPMHRDWKTWLLWTVLVGGAALIGALAVS
                     LLRKQ"
     gene            complement(3380..>3701)
                     /locus_tag="VFF05_04425"
     CDS             complement(3380..>3701)
                     /locus_tag="VFF05_04425"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZW51083.1"
                     /translation="LRSAHFLGSVPWTDELWRSALAQAALSIVWTLAGIAAMLLGKQR
                     GSRAVWLGGAVLMGVVIAKLLLIDREHLHDLAAIVGVLVVGVLLVAVGYFAPAPPRAS
                     GENA"
BASE COUNT          570 a         1166 c         1254 g          711 t
ORIGIN      
        1 gggattgatt ctcgcctggc gccagcgtgc gctgttgcgt gcagatgccg gcgtgcgttt
       61 ggccgtgatc ctgttcgcct gctactggct ggcggcgctc atttccgccc ccggcgcggt
      121 cgcgccggaa aagtcctgga gcacggtcgc ctatctgttg cgcttcctgc cgttcgcgct
      181 gttcgtctgc ctcgcattgc gtaggggtgc atggtggccg ctgttgaccg atgcgtgcgc
      241 cgcgatcgtc gtgctgtggc tgctggatgc ctgggtgcag atcctcaccg gttggagcct
      301 tgccggcgcg gaaactgcgg atcggctttc cggcattttc ggtgccggca acctcaagct
      361 cgggccggta ctggcggtgc tgtcgccgtt cgtcttcacc gccgcgcgcc ggcgctgggg
      421 ctggcgcgga ttggccatcg ccttcgtgtt tcagttgatg cccatcctct tcgccggctc
      481 gcgtgcggcc tggatcatgt atgggttggt ggcgctggct ttcgtgtgga tgcacacgcg
      541 cgcgccgctg cgtttcgtcg cgtggtcgct cgcggcggct tgcgcggcgc tgctcggcat
      601 cgccgtggca tggcagcatt cgggtcgttt cgacatgcgc ttcgaccgca ctttgctggc
      661 actgcaaggt tcgcaacagg cactcgatgc cgcttccgcc gggcgcctgc gcatctggcg
      721 cacggcgctg gcgatgagtg cggcccatcc cgtggccggc gtcggcgtgc gcggattccg
      781 attcgcctat ccgcagtacg cgcaacccgg cgatgccttt gtcgatgcgc aaaccgacga
      841 aggcgcattc cacgcgcacc agatcgtgct cgaagtgctc agcgaaaccg gcagcatcgg
      901 attgcttttg tggttgacgg gtgctttctg tgcgatccgc gcctggtgtc gcgcagaggc
      961 gcctgcgcgt gaacgtgcgc tcgcgccggt gttggcgctg gggacgatga catttccgct
     1021 gaatagccac ctcgcgtttt acgggacatc ggcgtggggg ttgctgttct ggtggctgct
     1081 cgcgctgtat tgcgcggcgt tgggaagcga cgaggcgccg ctatgaagcg gttttggcgg
     1141 cgcggcgtga tgtcgaagat ataatcgcac tgcacggcgt tgcagtgcag tatcccccga
     1201 ccgcgtccga ccgtatggct ttgcccatct cgatcttcat cattgcgttc aacgaagccg
     1261 cacgcatcgg tgcgacgcta tcgagcgtgc gcgagctgtc cgatgacgtc atcgtggtcg
     1321 attcgggttc gaccgacggt acgcaggata ttgccgcctc gttcggtgcc cgcgtcgtgc
     1381 acaacccatg gcccggttac ggcgcgcaaa aacgcttcgg cgaagaccag tgccgccacc
     1441 cgtggcgcct gaatctggat gctgatgaag tcgcgagcct ggatttgatc gcggaaatcc
     1501 actcgttgtt tgcgcacagc gagccggcgg cgggcgccta ttcggtgcgt atcgttgatg
     1561 tgattccggg cgattccaaa ccgcgcgcgt tcgcttacgc acacaaaaaa gtgaggctta
     1621 atcacaagaa cgccggccgc ttttcgccgt cacccgtgca tgatgccgtc gcactccatc
     1681 ccgccgtgaa agtgatgcgc cttcgcggcg ccgtccacca cttctcgatg cgcactctgg
     1741 gggagcaact gcacaaattc aatgcctaca ccgatgcgct ggtgcttgat ttgcgacggc
     1801 gaaacaagtc gattccgaca tggcgggttt tcgtcgaatt cccgatggcg ttttgcaaag
     1861 cctatttcat gcgccggcat tgcttgcgcg gcacttacgg atttttgacc gccatgaatt
     1921 acgcatattt ccggcatctg cgcgtggcga aattccacga ggcacgacga aacacgcacg
     1981 aatgacacat gccggcggct gacatcggcg cttgctcact gcttgcgcag caggctcacg
     2041 gccagcgcgc cgatcaacgc ggcgccaccg acgagcacgg tccacagcag ccaggtcttc
     2101 cagtcgcggt gcatcggtgc gggagccggc gtataggcgg tgacgcccag tagcgtcgcg
     2161 cgcgcgccga gcgtggccag tggcggttgc cagtcgttgc cgaatttggc gcgcagctgg
     2221 gcgagtgcgg catcgacagg ataatcgccg cggcgtgcgc ggacgctgcc ggccacgagc
     2281 cggtaaggcc cgttgccctg ggtgaggaag acgaagcgat ccgggcggaa cgcgacgcgc
     2341 aacgtcggta cgtggtcgag tggcgtggtc gactcgacga cccaatcctg cgtgcgcggc
     2401 gtcggcgcga tttcgatttc atcgttggcc aggaccgtgc catcctggcg caggcgaaac
     2461 gcggtgaagt ccgcgtgcaa gacgtgcggc gcgccgctgg acacccgcac gtgcgccagc
     2521 gagttgtccg tggccagttc gagtttcagc gcggcgaccg ccagcggtgc gggcagccga
     2581 tagcgaaagg acgaggtccc ggatgccggg ccttccccgg cctgcgccgg tagcggttgt
     2641 gcgtcgaccc agacacgggc cggctggatc agcgtcgagt gcgccagcac atgcgcacgg
     2701 acatgcaggc ttggcagcgg cgccccggtg tcgagtcgac gcaactgcag gaatttggca
     2761 tgcgcgccgt tgagtgcgat ctggcgccgg ctcaacgtgt tgccgttctg ttgcatggca
     2821 agcacgctgg cattggcgtt gagcacgcgc cattgctgca gatcgtcgct gccgctcacg
     2881 ctgaattgcg cggttacggc gttgctttgc tcgtcccagc tcagccacaa actgtcgatg
     2941 gcgccgtcct tgagtcctct ggcatcgaga atgatatggc cggcggtttg tgctgcattg
     3001 cgacggtcgt tgtcgacatc gagctgacgc agacgcccgt tgacatcacg tttgatccgc
     3061 aagtgcaggt tgtcgtcgtt cggactcatt ccggccagcg gtggcagacc gaatgcaggc
     3121 agctcgacat cgctcgtcgc cgccgaggaa aactgcactg cgcgcagcgc catcggcacg
     3181 gattcgccgg cagcattgac gacctcgaca tcgcgcaggt cggcggagcg caccgcggcg
     3241 tagacctcgg gcgtcaggtc gacctgccag gctgaactgt cgccgggcgt ttgcagcggc
     3301 caggcgtagg cgtagtcgtc cgcactggcc gcgtccgccg aagcggacaa cgagaggcaa
     3361 agcagcatga gggcgagttt catgcgtttt ctccgctggc gcgcggcggc gcgggcgcga
     3421 agtagccgac ggcgacgagc agcacgccga cgacgagcac cccgacaatg gcggcgagat
     3481 cgtgcagatg ctcgcggtcg atcagcagca gcttggcgat gacgacgccc atcaggacgg
     3541 cgccgccgag ccacaccgcg cgcgagccgc gctgcttgcc gagcagcatc gcggcgatgc
     3601 cggcgagcgt ccacacgatc gacagcgccg cctgcgcgag cgccgagcgc cacagttcat
     3661 cggtccaggg cacgctgccg aggaagtgcg cgctgcgcaa c
//
LOCUS       DASRVO010000352         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_114355, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000352 DASRVO010000000
VERSION     DASRVO010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114355"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1117)
                     /gene="hisC"
                     /locus_tag="VFF05_04430"
     CDS             complement(50..1117)
                     /gene="hisC"
                     /locus_tag="VFF05_04430"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZW51084.1"
                     /translation="MKLLERARPQILVLQPYSSARMEAGAAAIMLNANESPWPPVGAA
                     SALNRYPDPQPATLRAALARLYSVAPDNVLVGRGSDEAIDLLVRAFCREGRDAVVTAP
                     PTFGMYAVAARIQGASVIEVPRGDGFVLDAQAILAAVTEATKLVFVCTPNNPTGDSVP
                     LAVLAHLAGALRDRAVLVVDEAYIEFGDDAQSAISLFSRHDNIAVLRTLSKAHALAAA
                     RVGALIAAGPVVALLRRIQAPYPLPAPSIAAALAALEPAALADTDARIARIRGERERL
                     ARELLALPGVIAVLASAANFLCVRFVDAPGACRALASAGIVVRDVGGYPGLSGCLRIT
                     VGAAQENAALLGALGVCEAAA"
     gene            complement(1114..>1884)
                     /gene="hisD"
                     /locus_tag="VFF05_04435"
     CDS             complement(1114..>1884)
                     /gene="hisD"
                     /locus_tag="VFF05_04435"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZW51085.1"
                     /translation="CVFKIGGAQAIAAMAYGTESVPACDKLFGPGNAWVTEAKRQVSA
                     MCDGPAIDMPAGPSEVLVIADANANPEFVAADLLSQAEHGPDSQVLLIACERRVLLDA
                     RAALLRQVDFLPRASVARQALANSRFILAESVAETLAISNAYAPEHLILNLRDARAWL
                     DRVESAGSVFLGAWSPEAVGDYCSGTNHVLPTYGFARAWSGVSVASFQKQVSVQELSP
                     CGLREIGPDAMTLAHAEGLHAHERAVSLRLAALAGEGA"
BASE COUNT          333 a          651 c          636 g          264 t
ORIGIN      
        1 tcgacgatca gggtgccgtc gcgatcgagg aaaagcagct tgcgcgcatt catgccgccg
       61 cctcgcacac gccgagtgcg cccagcaggg ccgcgttttc ctgcgccgcg ccgaccgtga
      121 tgcgcaagca gccggaaagt cccgggtagc caccgacatc cctgacgacg atgccggccg
      181 aggccagggc gcggcatgcg ccgggcgcat cgacgaaacg cacgcaaaga aaattcgccg
      241 cggacgcgag caccgcgata accccgggca gcgcgagcaa ctccctggcg agacgttcgc
      301 gctcgccgcg aatccgggcg atgcgtgcgt ccgtatccgc caaagccgct ggctcgagcg
      361 ccgccagtgc cgccgcaata ctgggcgcgg gcaacggata cggggcctgg atgcggcgca
      421 acagcgccac gaccggcccg gcggcaatca gcgccccgac ccgtgccgcc gccagcgcat
      481 gcgccttcga caaggtgcgc agcacggcga tgttgtcgtg gcgcgagaac aatgagatgg
      541 cgctctgcgc gtcgtcgccg aattcgatat aggcctcgtc gacgaccagc acggcgcgat
      601 cgcgcaacgc gccggccagg tgcgcgagga ccgcgagcgg cacgctgtcg ccggtgggat
      661 tgttcggcgt gcagacgaag accagcttgg tggcttcagt cacggcggcg aggatggcct
      721 gcgcatccag cacgaatccg tcgccccgcg gcacttcgat cacggacgct ccctggatgc
      781 gcgcggcgac cgcatacatg ccgaaggtcg gcggcgcggt cacgaccgcg tcgcggcctt
      841 cccggcaaaa ggcgcgcacg agcaagtcga ttgcctcgtc gctgccacgt ccgaccaaga
      901 cgttgtcggg tgcgaccgag taaagccgcg ccagcgcggc acgcaatgta gccggctgcg
      961 gatcgggata acggttcagg gcggatgccg cgcccacggg cggccacggc gactcgttcg
     1021 cgttgagcat gatcgcggcg gcgccagctt ccatgcgcgc ggacgaatac ggctgcagca
     1081 ccagaatttg cgggcgcgcg cgttcgagca gtttcatgcg ccttccccgg cgagcgcagc
     1141 caggcgcagg gacaccgcgc gttcgtgcgc gtgcaatcct tcggcgtgcg caagcgtcat
     1201 tgcgtccggg ccgatttcgc gcaggccgca aggcgacagt tcctgcacgc taacctgctt
     1261 ctggaagctg gcgaccgaca cgccgctcca cgcgcgcgcg aagccatagg tcggcaacac
     1321 atggttggtg ccgctgcagt agtcgcccac tgcttccggc gaccaggcgc cgagaaagac
     1381 cgagcccgcg ctttcgacgc gatcgagcca ggcgcgtgcg tcacgcagat tcaggatcag
     1441 gtgctcgggt gcataggcat tgctgatggc cagcgtctcg gcaaccgatt ccgccaggat
     1501 gaagcggctg ttggccaagg cctgcctggc gaccgacgcg cgcggcagga agtcgacctg
     1561 gcgcaacagc gccgcacgcg cgtcaagcag cacgcggcgc tcgcaggcga tcagcaacac
     1621 ctgtgaatcc ggcccgtgct cggcctgcga gagcaggtcc gctgccacga actcgggatt
     1681 ggcattcgcg tcggcaatca caagcacttc ggaaggcccc gccggcatgt cgatcgcggg
     1741 gccatcgcac attgccgata cctggcgctt ggcttcggta acccaggcat tgcccgggcc
     1801 gaacaatttg tcgcaggccg gcacagattc ggtgccatac gccatcgccg cgatcgcctg
     1861 cgcaccgccg atcttgaata cgca
//
LOCUS       DASRVO010000353         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_244053, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000353 DASRVO010000000
VERSION     DASRVO010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244053"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1152
                     /locus_tag="VFF05_04440"
     CDS             <1..>1152
                     /locus_tag="VFF05_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HZW51086.1"
                     /translation="AYNNNPQLLEQRAKLRATDEGVPQALAGWRPTITFNANAAFNRT
                     GFEQPGTNTPTNPFACPGQGLGAACSTPTTFSSYETRQLALQVTQPLYRGGRTEAETR
                     QAINNVEATRAQTMAVEEQVFQAVVKGYLDVVRDQQLVEVARNNEEVLRKQLESTRDR
                     FRVGEVTRTDVAQAESSLAQATAQRITDEGNLRNSEAEYTQAVGHPPGRLLQPRERPA
                     LPAALDRALQLAANDNPNVIAAKFTELAARANVSVVRSQLLPQLSLVGSLSRAYAPSV
                     VLQGAREDAASLALQLTVPLYQGGKVYSQTRQAEQTVGQQQSEVDLQRRNAVQTATQN
                     WETLKAARAAIASFGTAVRAAQIALEGTQQEALVGTRTVLDVLIAEQQL"
BASE COUNT          226 a          388 c          375 g          163 t
ORIGIN      
        1 tgcctacaac aacaacccgc agctcctgga gcagcgcgcc aagttgcgcg ccaccgacga
       61 gggcgtgccg caggcactgg ctgggtggcg cccgaccata acgttcaacg ccaacgccgc
      121 ctttaaccgc accggctttg agcagccggg cacgaatacg ccgacaaatc cattcgcctg
      181 cccaggacag gggcttggcg cggcctgttc gacccccacc acattttcga gctacgagac
      241 tcgccaactg gccttgcagg tcacgcagcc gctctatcgc ggcggtcgca ccgaggcaga
      301 gacacgtcag gcgatcaaca acgtcgaggc aacgcgtgcc cagacgatgg cggtcgagga
      361 acaggtattt caggcggtcg tcaaaggcta tctcgatgtc gtgcgcgacc agcagctcgt
      421 cgaagtcgcg cgcaacaacg aagaagtgct gcgcaagcag cttgaatcga cgcgagaccg
      481 ctttcgcgtc ggcgaggtaa cgcggaccga cgtggcgcag gcggagtcgt ctttggcgca
      541 agccaccgcc cagcgtatca ccgatgaagg caacctgcgc aacagcgagg ccgaatatac
      601 tcaggcggtc ggccatccgc ccggccggtt gctccagccg cgcgagcgcc cggcactgcc
      661 ggcggcgctc gaccgggcgc tgcagctcgc cgccaacgac aatccgaacg tcattgcggc
      721 gaagtttacc gaactggcgg cgcgtgccaa cgtgtcagtc gtgcgcagcc agcttctgcc
      781 ccagctatcg ctggtcggca gcctgtcgcg cgcctacgcg ccgtcggtcg tgttgcaagg
      841 cgcgcgcgaa gacgcggcga gcctcgcact gcaattgacg gtgccgctct atcagggggg
      901 aaaggtctat tcgcagaccc gacaggccga gcagaccgtg ggccaacagc aaagcgaagt
      961 ggacttgcag cgccgcaacg cggtgcagac cgcgacccag aactgggaga cgctaaaggc
     1021 ggcgcgcgcc gcgatcgcct ctttcggcac cgcagtgcgg gcggcgcaga tcgcgctcga
     1081 aggcacccaa caggaggcgc tggtcggcac gcggaccgtg ctcgacgtgt tgatcgccga
     1141 gcagcagttg tt
//
LOCUS       DASRVO010000354         1381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_114450, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000354 DASRVO010000000
VERSION     DASRVO010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114450"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..870)
                     /locus_tag="VFF05_04445"
     CDS             complement(58..870)
                     /locus_tag="VFF05_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZW51087.1"
                     /translation="MDDVLVLVGNCETWQPHVRYAARLAAKLRGSLNGIYVTPSAIPV
                     PTDASAAFSREIIEIYREETEQALRAESPFRQWARREGIEHSTWRIARGSPAEVLESA
                     AKWHDLVILEAHAEPFGGDILETGSAIVRADLPCVVVPRGMDKANLDTIAIAWNGSIQ
                     SARAIHAALPLLQQARRIAILRVDDADAAGAIDRIEPGEFLRRHGLRCEHFDLQGRAD
                     DAGTALLAGAAQIFADLLVLGAYGTGRFSEWIFGGVTRHVLQHVALPLFMRH"
     gene            complement(927..>1381)
                     /locus_tag="VFF05_04450"
     CDS             complement(927..>1381)
                     /locus_tag="VFF05_04450"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HZW51088.1"
                     /translation="GRTVMADTWILVADSARARVFEVTREDPTLREVAAFTNPEGRIA
                     QRELRHDRLPRVNESMGHARHAIEPHTSLKQKSTERFVRMLRDELERGHATHSYEHLV
                     LVAAPRFMGALHGALHDSLQKCVVAEVKRNLTPMSPAKIREHLPPRLA"
BASE COUNT          235 a          444 c          448 g          254 t
ORIGIN      
        1 gcaaagcaat catccggcat cttgatcttg atcaagtgcc ggcggtgcct tcacgggtca
       61 gtgacgcatg aacaatggca gcgccacgtg ctgcaggacg tggcgcgtga cgccgccgaa
      121 tatccattcg ctgaaacgcc ccgtgccgta ggcgcccaga acaagcagat ccgcgaagat
      181 ctgcgccgcg ccggcgagca aggcagtccc ggcatcgtcg gcgcgacctt gcagatcgaa
      241 atgctcgcag cgcagaccat gccgccgcag gaattcaccg ggttcgatcc ggtcgatcgc
      301 ccccgccgca tcggcatcgt cgacgcgcag gatagcaatg cgtcttgcct gttgtagcag
      361 gggcagcgcc gcgtgtatcg cgcgtgcgga ctggatcgat ccgttccagg cgatggcgat
      421 ggtgtccaga ttcgccttgt ccattccccg gggaaccacg acacagggca gatcggcccg
      481 aacgatcgcg gatccggttt caagaatgtc gccaccgaac ggctcggcgt gcgcctcaag
      541 gatcacgaga tcgtgccact tcgccgccga ttcgagtact tcggctggag atcccctggc
      601 gatacgccac gtgctgtgct cgatgccctc ccggcgggcc cactgcctga acggcgactc
      661 ggcgcgcaat gcctgttccg tctcctcgcg atagatctcg atgatctcgc gggaaaaggc
      721 ggccgacgca tcggtcggca cgggaatcgc ggatggcgtc acataaatgc cgttgagcga
      781 gccgcgcagc ttcgccgcga gcctggccgc gtaacggacg tgcggttgcc atgtttcgca
      841 attcccgacc agtaccagta cgtcatccat cggatggcct gctgtcgcgg agccggagcg
      901 atggtagtga ctgcatcatt agcaggtcac gccagccgcg gcgggagatg ctcgcgaatc
      961 tttgcgggcg acatcggcgt cagattgcgc ttcacttcgg cgaccacgca tttctgcagc
     1021 gaatcgtgga gcgctccgtg cagtgctccc atgaagcgcg gcgcggcgac gagcacgagg
     1081 tgctcgtagc tgtgcgtcgc gtggccgcgc tcgagctcgt cgcgcagcat gcgcacgaag
     1141 cgctcagtgc ttttctgttt gagcgaggtg tgcggctcga tcgcgtggcg cgcgtgcccc
     1201 atgctttcgt tgacgcgcgg caggcggtcg tggcgcagtt cccgctgcgc aatgcggcct
     1261 tcgggattcg tgaacgcggc gacttccctc agcgtggggt cttcgcgcgt gacttcgaac
     1321 acccgagcgc gggccgaatc ggccaccagg atccaagtgt cggccatgac tgttctccca
     1381 t
//
LOCUS       DASRVO010000355         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_244160, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000355 DASRVO010000000
VERSION     DASRVO010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244160"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFF05_04455"
     CDS             complement(<1..569)
                     /locus_tag="VFF05_04455"
                     /inference="COORDINATES: protein motif:HMM:NF040152.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HZW51089.1"
                     /translation="MDIKLMEQPPSQRSSALFADIAERGTTDNLLRTAQQHHVALSSM
                     ADTKANIIITMSSIVLTVILSRLGDPVIGVAALILGTFTLVALLLAILAVLPKFRPPH
                     LKSDGSLPPDFNLLFFGHFGVLTRERYLQEMAHALARDGSPYATWAADIYSLGVYLAH
                     HKYRYLRWSYLFFLAGFVLACAEQAWRLLT"
     gene            614..1510
                     /locus_tag="VFF05_04460"
     CDS             614..1510
                     /locus_tag="VFF05_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003472081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZW51090.1"
                     /translation="MIGSARRLGNRLLLAAGVLTLAGCADAFFAAVDRLEYSRNVVER
                     DRIVFDAGHDLALDAYTPTGASGDPVVVFFYGGSWSVGKRHWYRYVGDALAGNGVVVL
                     IPDYRKYPDVRFPAFMHDAAHAVAWAREHAAEFGGDPRRIFVMGHSAGGQIAALLACD
                     TRYLAAVGMQPRDLAGMIGVAGAYAFLPFVEDEPEIFGDDAQGRYDSQPINFVDGDEP
                     PMLLLQGTDDEEVPPNNAQGMAERAQAMDGTATLKLYPDVGHNRILLALARGHEAHIP
                     TLADTLAFIARIDAADKTAPMR"
     gene            complement(1520..2080)
                     /locus_tag="VFF05_04465"
     CDS             complement(1520..2080)
                     /locus_tag="VFF05_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZW51091.1"
                     /translation="MNLNEIKATIPDYAKDLRLNLDSVLAETGAPGLTQKQIGLIALA
                     GAIASRHVPLIDAIAMEAAKSASPQELDGARSAAAIMAMNNVYYRFTHLVSDPEYQTL
                     RAGLRMNAMANPGCDKLDFELCSIAVSAINGCGQCMDSHEKTLKKHGVSAQAIQGAVR
                     IAAMVHAVAVTLEQTASAISEFAAAA"
     gene            complement(2133..2675)
                     /locus_tag="VFF05_04470"
     CDS             complement(2133..2675)
                     /locus_tag="VFF05_04470"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZW51092.1"
                     /translation="MLTVGDKFPEFKLKATVAIDSLDTAFAEIDNDTYKGKWTLVFFY
                     PKDFTFVCPTEIKGFGDLNAQFRDRDCQVLAASTDTEFVHLAWRKDHKDLRFLPFPML
                     ADTAKRLSSALGILDAEEGVAKRATFLVDPEGVIRFAYVTDGSVGRNPAEVLRVLDAL
                     QTDELCPCNWSRGEETLKAA"
     gene            2809..>3348
                     /locus_tag="VFF05_04475"
     CDS             2809..>3348
                     /locus_tag="VFF05_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HZW51093.1"
                     /translation="MNLRDLRYLVALAEHRHFGRAAAASFVSQPTLSTQIKKLEDELG
                     VALVERTPRKVLLTEVGREIAQRAREVLNEVEQIKAIARRTLDPESGTVRLGIFPTLG
                     PYLLPHVVPAIRERFPRLELLLVEEKTEVVLRLLREGRLDAGVLALPIHDDQLHAEFL
                     FEEPFLLAVPEQHELSRRKA"
BASE COUNT          597 a         1045 c         1092 g          614 t
ORIGIN      
        1 gtgagcaggc gccaggcctg ttcggcgcag gcgagcacga atccggcaag gaagaacagg
       61 taactccagc gcaagtagcg gtatttgtga tgcgccaggt acacgcccag cgaatagatg
      121 tcggcagccc aggtcgcata cggcgaaccg tcgcgtgcca gcgcgtgcgc catttcctgc
      181 aggtagcgtt cgcgcgtaag cacgccgaaa tggccgaaga acaacaggtt gaagtcgggc
      241 ggcagcgaac cgtcggactt caaatgcggc ggccggaact tcggcagcac ggcgaggatg
      301 gcgagcagca gcgcgacgag ggtgaacgtg ccgaggatca gcgcggcgac gccgatgacc
      361 ggatcgccca gccgactcag gatcaccgtc agcacgatcg aggacatggt gatgatgatg
      421 ttggccttgg tatcggccat gctcgacagc gccacgtggt gctgttgcgc ggtgcgcaag
      481 aggttgtccg tggtgccgcg ctcggcaatg tcggcgaaca gcgcgctgga gcgttgtgac
      541 ggtggttgtt ccatcagctt aatgtccatc aacttaatgt ctatcaacat cagcacatga
      601 cagaatacgc cgcatgatag gaagcgcgcg ccggcttggc aatcgtcttt tgctggccgc
      661 cggcgtgctc acgctcgccg gctgcgcgga cgcgtttttc gctgccgtgg accggctcga
      721 atactcgcgc aacgtagtcg aacgcgaccg catcgtgttc gatgccgggc acgacctcgc
      781 gctcgatgcc tatacgccga ccggtgcgag cggagatccg gttgtggtgt tcttctacgg
      841 cggcagctgg agcgtgggca agcgccactg gtaccgctat gtcggcgacg cgctggcggg
      901 caatggcgtg gtcgtgttga ttccggacta tcgcaagtat ccggacgtgc gctttcccgc
      961 cttcatgcac gacgccgcgc atgcggtggc gtgggcgcgc gagcatgccg ccgaattcgg
     1021 cggcgatccg cggcgcattt tcgtgatggg acattccgcc ggcggccaga tcgccgccct
     1081 gctcgcctgc gacacgcgct atctcgctgc cgttggcatg cagcctcgcg atctcgccgg
     1141 catgatcggc gtggccggcg cctatgcgtt tctgcccttc gtcgaagacg agccggaaat
     1201 cttcggcgac gacgcgcaag gccgctacga ttcgcagccg atcaatttcg tcgatggcga
     1261 cgagccgccg atgctgttgc tgcaaggcac ggacgacgag gaagtgccgc cgaacaacgc
     1321 gcaggggatg gccgaacgcg cgcaggcgat ggacggcacg gccacgctga agctgtatcc
     1381 ggatgtcggc cacaaccgca tcctgctcgc cctcgcgcgc ggccacgagg cgcatatccc
     1441 gacgctggcc gatacgctgg cgttcatcgc gcgcatcgat gccgccgaca agacggcacc
     1501 gatgcgttga tcttcgcgct caagccgccg cggcgaattc ggaaatcgcc gaggcagtct
     1561 gttccagcgt taccgccacc gcgtgcacca tcgccgcgat gcgcaccgcg ccctggatcg
     1621 cctgcgcgga cacgccgtgc ttcttcagcg tcttctcgtg cgaatccatg cactggccgc
     1681 agccgttgat cgcggaaacc gcgatcgaac acaactcgaa atcgagcttg tcgcaacccg
     1741 ggttggccat cgcgttcatg cgcaatccgg cgcgcagggt ctggtattcc gggtccgaca
     1801 ccaggtgcgt gaagcggtag tagacgttgt tcatcgccat gatcgcggcg gcgctgcggg
     1861 ccccgtcgag ttcctgcggg ctggcggact tcgccgcttc catggcaatc gcatcgatca
     1921 atggcacgtg ccgcgacgcg atggcgccgg ccagcgcgat caggccgatc tgcttctggg
     1981 tcaagcccgg cgcgccggtt tcggcgagca cggaatcgag gttcaggcgc aagtccttgg
     2041 cgtaatcggg aatcgtggct ttgatttcgt tcaaattcat ctcgttctcc taaaagccgg
     2101 cggcgcaatg ccgccggctt gcgtcggtgg gatcaggccg ccttcaacgt ctcttcgccg
     2161 cggctccagt tgcacggaca cagctcgtcg gtctgcaagg catcgagtac gcgcaatact
     2221 tcggccggat tgcggccaac gctgccgtcg gtgacgtagg cgaagcggat cacgccttcc
     2281 ggatcgacga ggaaggtcgc gcgtttggcc acgccttctt ccgcgtcgag gatgccgagc
     2341 gcggaactca ggcgcttggc cgtgtcggcc agcatcggga acggcaggaa gcgcaggtcc
     2401 ttgtgatctt tgcgccaggc caggtgcacg aactcggtgt ccgtgctcgc ggcaaggacc
     2461 tggcagtcgc ggtcgcggaa ctgcgcgttc agatcgccaa agcccttgat ctcggtcggg
     2521 cagacgaagg tgaaatcctt cggatagaag aacaccagcg tccacttgcc cttgtaggtg
     2581 tcgttgtcga tctcggcaaa cgccgtgtcg aggctatcga tggccacggt ggccttgagt
     2641 ttgaattcgg gaaacttgtc gccgacggta agcatgctgg actcctaatg tcgtggttgg
     2701 aaaggcggcc cggctgggca cggtatcaag ataggggcga aacgatgatc gctcaaatcg
     2761 attgatgcta tactttcgat aggatttgta aatgattgga ggcatcccat gaacctgcgc
     2821 gacctgcgtt acctcgtcgc cctggccgag caccgtcatt tcggccgcgc cgctgcggcg
     2881 agcttcgtca gccagccgac cctgtccacc cagatcaaga aactcgagga cgaactcggc
     2941 gtcgccctgg tcgaacgcac gccgcgcaag gtgctgctca ccgaagtcgg ccgcgaaatc
     3001 gcgcaacgcg cgcgtgaggt gctcaacgag gtcgaacaga tcaaggcgat cgcacggcgc
     3061 acgctcgatc cggaatccgg cacggtgcgc ctgggcatct tcccgacgct gggaccgtac
     3121 cttttgccgc atgtggtgcc agcgattcgc gagcgctttc cgcgcctgga actgttgctg
     3181 gtcgaggaaa aaaccgaagt tgtgctgcgt ctgctgcgcg agggccgcct cgacgccggc
     3241 gtgctcgccc tgcccatcca cgacgatcag ctgcatgccg aattcctgtt cgaggaaccc
     3301 ttcctgctcg ccgtgcccga gcagcacgaa ttgagccggc gcaaggcg
//
LOCUS       DASRVO010000356         5223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_270607, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000356 DASRVO010000000
VERSION     DASRVO010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270607"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFF05_04480"
     CDS             <1..818
                     /locus_tag="VFF05_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW51094.1"
                     /translation="LVVESRGDATMPALVFTHGFGQTRHAWDASAATLARAGWHCLAM
                     DARGHGDSGWRSDGRYDFSQFVDDLVRLARVPADKPILVGASMGGLLGLVAQAEHDVF
                     RALVLVDITPRWEIAGVERILAFMRAHPQGFASVEEASDAIAHYLPHRAQRKSPERLR
                     SLLVTDAGGRLRWHWDPRLLDTIATGSEREQARLLDAARQIRVPTLLVSGERSDVVSD
                     DTIAEFLRCVPHAQHVRVARATHMIAGDENAAFTKAVEHFVTPLRNNRTEDRP"
     gene            815..3283
                     /locus_tag="VFF05_04485"
     CDS             815..3283
                     /locus_tag="VFF05_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZW51095.1"
                     /translation="MIHWYPLIALLVVSLACAYLRTRLVIWTVAGFAAVIGVGLLAGS
                     HWLAIAITTLVFALVVLPLNIPELRRRRITAPLLGIYQKITPQLSDTERIALEAGTVG
                     FEGELFSGKPDWSKLLKQPKPELSVEEQAFLDGPCEELCRIVDDWQISHELSDLPPEV
                     WDFVKQKKFFGMIIPKRYGGLEFSALAQSAILQKLMSMSGALSSTVGVPNSLGPGELL
                     LHYGTPEQRDYYLPRLADGREIPCFALTGPHAGSDATSIPDYGIVCKGEWQGGNVVGV
                     RLTFDKRYITLAPAATIIGLAFRMYDPDHLLGDNEDLGITLALIPRETRGLEIGRRHF
                     PLNAAFLNGPLRGKDVFVPLSQLIGGEKMAGQGWRMLVECLSIGRGITLPANASGGVR
                     MGAIATGAYARIRKQFGMAIGRFEGVEEALARIGGYAYAITALSRASAAAVDRGEKPA
                     VGSAIAKYHCTELGREVARDVMDVHGGKAVQLGPSNYAGRSWQTAPIAITVEGANIMT
                     RSLMIFGQGAIRCHPFVLKELQAAQIADYRQRLTEFDRVLFRHVGFAISNAARSFVLG
                     LSHARIGGAPGDKYTRRFYRKLNRYAANLALAADTAMLTLGGRLKFKEKISARLGDVL
                     SNLYIASAMLKRYEDQGRPVADQPLLAWAFHDCAYRMQSALDGTLRNFPIRPVAWLLR
                     ALIFPIGRREAPPSDRLGHRVATILCAPNDARTRLAEWIYLTPSANNPVGRMNALLPD
                     VIAAEPVERKFAKALKSGTIKALDAAAQLAEAEQTGALSAEERKLLERVRAAAQEFID
                     VDDFDPAELRAAVIKPKSELRNVA"
     gene            complement(3391..4695)
                     /locus_tag="VFF05_04490"
     CDS             complement(3391..4695)
                     /locus_tag="VFF05_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51096.1"
                     /translation="MSDPHSQATATRTARWLRVERGEWPAVLLGFVYFFFVLTAYYVI
                     RPVRDQLSAAVGSIALPIFYSATFVVTLALTPLFGALVARFRRRRFVPVVYVLCIAGT
                     LAFVPLFEMQAQIGPRLLGTIFFVWGSVFNLFVVAVFWSFMADLFDAEQSRRLFPLIA
                     LGGPLGAIAGPILTRELVFRVGVSGLLCVSAAALAATLVCIFALLRWSRAHPVRGDAR
                     RDERIIGGGVMAGAVITFSSPLLFRLAVLMLLGDSVGTVVYALLADYSHAHFHTAAAR
                     IDFAASLDLGTNLLQAALQVLLTRELMVRLGPVWALILDGVVKAVMLAGLIVFGAGWI
                     AVVAIITRASAYGVFKPANDSLYTLVDAETRYKGKNFIDTVVWRFGDVVITSGLNALR
                     GAGVAIAGLAGLSILAAAASSFLGWRVGRARELQGDAAVPAR"
     gene            4750..>5223
                     /locus_tag="VFF05_04495"
     CDS             4750..>5223
                     /locus_tag="VFF05_04495"
                     /inference="COORDINATES: protein motif:HMM:NF015441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HZW51097.1"
                     /translation="MLTSIHIYPLKSCAPLPLDSARVEARGLEHDRRWMVVGANGKFL
                     TGRQHPRLTLIRVVPAGVALHLRAPDMPELRLAPTPDRKRREVEVWSSRVAALDADDA
                     ANGWISAFLGIPAHLVHMDADCLRAIDPDHSLPGDTVSFADGYPLLAISQASLDML"
BASE COUNT          956 a         1810 c         1639 g          818 t
ORIGIN      
        1 cgctcgtggt cgagtcacgc ggcgacgcca cgatgccagc gctcgtcttc acccacggct
       61 tcggccagac ccggcatgcc tgggatgcca gcgcggcaac cttggcgaga gccggatggc
      121 attgcctggc gatggatgca cgcggccacg gcgatagcgg ctggcgcagc gacggccgat
      181 acgatttctc ccagttcgtc gacgatctgg tgcggctcgc gcgggttccc gccgacaaac
      241 ccatccttgt cggcgcctcg atgggcggac tgctgggcct ggtcgcacag gccgaacacg
      301 acgtattccg cgccctcgtg ctggtcgata tcacgccacg ctgggaaatc gccggcgtgg
      361 aacgcatcct cgccttcatg cgcgcccacc cgcagggttt cgcctcggtc gaggaagcca
      421 gcgacgcgat agcgcactac ctgccgcatc gtgcgcagcg caaatccccc gagcgcttgc
      481 gcagcctgct cgtgaccgac gccggcggac gcctgcgctg gcactgggac ccgcgcctgc
      541 tcgacacgat cgccaccggc agcgagcgcg aacaggcgcg cctgctcgat gccgcacgcc
      601 agattcgcgt gccgaccctg ctcgtcagcg gcgagcgcag cgacgtggtg tccgacgaca
      661 ccatcgccga attcctgcgc tgcgtgccgc acgcacagca tgtgcgcgtg gcgcgcgcca
      721 cgcacatgat cgccggcgac gaaaacgccg ccttcaccaa agcggtcgag cacttcgtca
      781 cgccgctgcg caacaaccgg accgaggata gaccatgatt cattggtacc cgctcatcgc
      841 cctgctcgtg gtttcgcttg cctgcgccta cctgcgcaca cgcctcgtca tctggactgt
      901 cgccggtttc gccgccgtca tcggcgttgg cttgctggcc ggcagccatt ggctggcgat
      961 tgcaattacc acgctggtgt tcgccctggt cgtgctgccg ctgaacattc cggaactccg
     1021 ccgccgccgc atcaccgcgc cgctgctggg catctaccag aagatcacgc cgcaactttc
     1081 cgacaccgaa cgcatcgcgc tggaagcggg caccgtcggt ttcgaaggcg aactgttcag
     1141 cggcaagccc gactggtcaa agctgctcaa gcagcccaag ccggaattgt cggtggaaga
     1201 acaagccttt ctcgacggac cctgcgaaga gctgtgccgc atcgtcgacg attggcagat
     1261 ttcccacgag ctgtccgatc tgccgccgga ggtctgggac ttcgtcaagc agaagaaatt
     1321 cttcggcatg atcatcccga agcgctatgg cggtctggaa ttctctgcgc tggcgcaatc
     1381 ggcaattctg caaaagctga tgagcatgtc cggcgcgctg tcgtccacgg tcggcgtgcc
     1441 gaactcgctc ggacccggtg aactgctgct gcattacggc acgcccgagc agcgcgatta
     1501 ctatttgccg cgcctcgccg acgggcgcga gattccctgt ttcgccctga ccggtccgca
     1561 tgcgggttcc gacgccacct cgattcccga ctacggcatc gtctgcaagg gcgaatggca
     1621 gggcggcaac gtggtcggcg tgcgcctgac tttcgacaag cgctacatca ccctcgctcc
     1681 ggccgccacg atcatcggtc tcgccttccg catgtacgat ccggatcatc tgctcggcga
     1741 caatgaagac ctcggcatca cgctggcact gattccgcgc gagacgaggg gtctggaaat
     1801 cggccgccgg cattttccgc tcaacgccgc gttcctgaac ggcccgctgc gcggcaagga
     1861 cgtgttcgtg ccgctgtcgc aactgatcgg cggcgagaag atggccggcc agggctggcg
     1921 catgctggtc gaatgcctgt ccatcggccg tggcatcacc ttgcctgcga acgcgtcggg
     1981 tggcgtgcgc atgggcgcga tcgcgaccgg cgcctacgcg cgcatccgca agcaattcgg
     2041 catggccatc ggccgcttcg agggcgtcga ggaagcactc gcgcgcatcg gtggctatgc
     2101 ctacgcgatc acggcactgt cgcgcgccag tgccgctgcc gtcgaccgcg gcgagaaacc
     2161 ggcggtggga tcggccatcg ccaagtacca ctgcaccgaa ctcggccgcg aagtcgcgcg
     2221 cgacgtgatg gacgtgcacg gcggcaaggc cgtgcagctc ggcccatcca actacgccgg
     2281 ccgttcgtgg caaaccgcgc ccatcgcgat caccgtggaa ggcgcgaaca tcatgacgcg
     2341 cagcctgatg atcttcggcc agggcgcgat ccgctgccat ccgttcgtgc tcaaggaatt
     2401 gcaggcggcg cagattgccg actaccgcca gcgcctgacc gaattcgacc gcgtgctgtt
     2461 ccgccacgtc ggcttcgcga tttccaacgc cgcgcgcagt ttcgtgctcg gcctcagcca
     2521 cgcacgcatc ggcggcgcgc cgggcgacaa gtacacgcgg cgcttttacc gcaagctcaa
     2581 ccgctacgcc gcgaacctgg cgctcgccgc cgacaccgcc atgctcacgc tcggcggcag
     2641 gctcaagttc aaggaaaaga tttctgcacg ccttggcgac gtgctgtcca acctctacat
     2701 cgccagcgcc atgctcaagc gttacgaaga tcagggccga ccggtcgccg accagccgtt
     2761 gctggcctgg gcgttccacg attgcgcgta ccgcatgcag agcgcgcttg acgggacctt
     2821 gcgcaatttc ccgatccgtc cggtggcgtg gctgctgcgc gcgctgatct tccccatcgg
     2881 ccgccgcgaa gcgccgccgt ccgaccgcct cggccaccgc gtcgccacga tcctgtgcgc
     2941 gccgaacgat gcgcgcacgc gcttggccga atggatttac ctcacgccga gcgcgaacaa
     3001 tccggtcggg cgcatgaacg cgctgctgcc cgatgtcatc gccgccgaac cggtcgagcg
     3061 caagttcgcc aaggcgctca agtccggcac gatcaaggca ctggatgccg ctgcacaact
     3121 cgccgaagcc gagcaaaccg gcgcgctcag cgccgaggaa cgcaagctgc tcgaacgcgt
     3181 gcgtgcggcc gctcaggagt tcatcgacgt ggacgacttc gaccctgcag aattgcgtgc
     3241 ggcggtaatc aagccaaagt cggagttgcg caacgtggcc tgagaaattg tcggcgggat
     3301 gcgtcgcagc gcgacgtctc ccgccctacg gattttgcaa ccgcccacgt agggcgggag
     3361 tagcgaagcg tatcccgccg cgagtgccgg tcaacgtgcg ggaacagccg cgtcgccttg
     3421 cagctcccgc gcccttccta cccgccagcc caggaaactc gacgccgctg cggcgagtat
     3481 cgacaatccg gcaaggcccg cgatcgcgac gcccgcaccg cgcagggcgt tcaatccgct
     3541 ggtgatgacg acgtcgccga agcgccagac cacggtatcg atgaagttct tgcccttgta
     3601 gcgcgtctcc gcgtcgacca gggtatacaa actatcgttg gccggtttga acacgccgta
     3661 ggcgctggcc cgcgtgatta tggcaacgac ggcgatccag ccagcgccga acacgatcag
     3721 gccggcgagc atcacggcct tgaccacgcc gtccaatatc agcgcccaga ccggtcccag
     3781 gcgcaccatc aactcacgcg tgagcaggac ctgcaacgcg gcctgcagca ggttggtgcc
     3841 gagatcgaga ctggcggcga aatcgatgcg cgccgcggcg gtgtggaaat gcgcatggct
     3901 gtaatcggcc aacagcgcgt agaccaccgt accgacgctg tcgccaagca acatcagcac
     3961 ggcaaggcgg aacagcagcg gcgaagaaaa cgtgatcacc gcgcccgcca ttacgccacc
     4021 gccgatgatg cgctcgtcgc gccgtgcatc gccgcgcacc ggatgcgcgc gcgaccagcg
     4081 caacagcgcg aagatgcaca ccagcgtcgc ggcgagtgcg gccgccgaca cgcacagcaa
     4141 cccggaaaca ccgacgcgaa aaaccagttc gcgcgtgagg atcggcccgg cgatggctcc
     4201 gagcggaccg ccgagggcga tcagcggaaa cagccgccgc gattgctcgg catcgaacag
     4261 gtcggccatg aagctccaga acacggcgac cacgaacagg ttgaagacgc tgccccagac
     4321 gaaaaaaatc gtgccgagca ggcgggggcc tatctgtgcc tgcatctcga acaggggcac
     4381 gaaggcgagc gtgccggcaa tgcacaggac gtacaccacg ggcacgaagc ggcggcgccg
     4441 gaaacgcgcg accagagcgc cgaacaacgg cgtcaatgcc agggtgacga cgaacgtcgc
     4501 gctgtagaaa atcggcagtg cgatcgaacc gaccgccgcc gacaactggt cgcgcaccgg
     4561 acggatgacg tagtacgccg tcagcacgaa gaaaaaatac acgaagccga gcagcaccgc
     4621 gggccactcg ccacgctcca cgcgcagcca gcgcgccgtg cgggttgcgg tggcctggga
     4681 atgcggatcg ctcatccatg cccctcgacg tgactggcgc gtagcgtaac atgcacccgc
     4741 atgaccgcca tgctcacttc catccacatc tatccgctca agtcctgcgc gccgctgccg
     4801 cttgattcgg cacgcgtgga agcacgtggt ctggaacacg atcggcgctg gatggtagtc
     4861 ggtgcgaacg ggaaattcct gaccggccgc cagcatccgc gcctgacctt gattcgcgtc
     4921 gtaccggcgg gcgttgcgtt gcatctgcgc gcaccggaca tgccggaact gcgactcgcc
     4981 ccgacgcctg atcgcaagcg ccgggaagta gaagtctgga gtagtcgcgt cgcagcgctc
     5041 gacgcggacg acgcagcaaa cggctggatc agcgcgttcc tgggcattcc cgcgcacttg
     5101 gtgcacatgg acgccgactg cctgcgtgcg atcgatccgg atcattcact gcccggcgac
     5161 acggtcagtt tcgccgacgg ctacccgttg ctcgcgatct cgcaggcgtc gctggatatg
     5221 ctc
//
LOCUS       DASRVO010000357         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_193270, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000357 DASRVO010000000
VERSION     DASRVO010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193270"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="VFF05_04500"
     CDS             complement(<1..2029)
                     /locus_tag="VFF05_04500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW51098.1"
                     /translation="MLWAALNRPAQPANWSGEIGGFAYVPYQRYQNPNKQIYPNTDQI
                     DADLKLLSKYSKRLRTYAALENPAVAAIAKKYGMTVMAGAWLDSRWQNDENEIAALIA
                     ETRANDNVTRVIVGNETLLRNDMGVDTLISYIDRVRAQVKVPVSTAEPWHIWLKYPQL
                     AKHVDFITVHLLPYWEGYPRKDAIGLQVLMRYYQLQSAFPGKHIVIGEIGWPSSGDRV
                     KGATASIANESLFIRQWLNVARERGFDYYLMEAIDQPWKEPFEGRVGAYWGMFGADRT
                     PKFSLTGPVIEDPAWPWKAGIACLLALLPMFWFARHFMRFKVVGLTFFCVLIQLSASL
                     LVWSATLPYAFYLDPFDWTMLTILAPAQVAIILILLINGFEFTEVIWRGKWLRHFGLL
                     EPKPGDAQPFVSIHLACCNEPPEMVILTLDSLAALDYENFEVLVIDNNTRRDEVWQPV
                     EKHCKKLGPKFRFFHLNPWPGFKAGALNFGLKETDARAEVVAAIDADYAVRADWLRAL
                     TGYFADPKVAVVQCPQAHRDYEHNAFRRMTNWEYDGFFRIGMHHRNERNAIIQHGTMT
                     MVRRGALEATGAWSEWTICEDAELGLRLMHSGYELVYVDEIMGRGLTPADFTAYKSQR
                     YRWAFGAMQILKARWHWMTERGPLTWGQRFHFLTGWFSWFADALHLAFTLLA"
     gene            complement(2213..2923)
                     /locus_tag="VFF05_04505"
     CDS             complement(2213..2923)
                     /locus_tag="VFF05_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZW51099.1"
                     /translation="MKRLRSFLPLLILIAIGIALLSTGVLNRFRPETLAREQANLQAQ
                     IAAHPIHAGLIHVGVTTLAIATGVPGVLVVILAGGMLFGVFWGTILSTTGATIGALIL
                     FLASRHAFGTQSGASAPALVDKLRGGYLAHPVSYTCFLRLVPFFPFGGVTVALAWLRC
                     PLWLFLAATAGGGALMVGIETVLGAGLAKNIGTNHEVNLSLFSDPLVIAPLVAMGLLA
                     LVPVAIGKWREARKLKSG"
     gene            complement(2995..>3202)
                     /locus_tag="VFF05_04510"
     CDS             complement(2995..>3202)
                     /locus_tag="VFF05_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51100.1"
                     /translation="LDHALLTRTAWTDFVSLHNAHGNADTSEAGPEVTDSSTPARSAD
                     HDGQVLTLTFDRLFSDGFESDTCR"
BASE COUNT          601 a          964 c         1076 g          561 t
ORIGIN      
        1 gcgcgagcag ggtgaaggcc aggtgcaggg cgtccgcgaa ccacgagaac cagccggtca
       61 ggaaatggaa gcgctggccc caggtcagcg gaccacgttc ggtcatccag tgccagcgcg
      121 ctttgagaat ctgcatcgcg ccgaacgccc agcggtagcg ttgcgatttg taggcggtga
      181 aatcggcggg cgtaaggccg cggcccatga tctcgtcgac gtagaccagc tcgtagcccg
      241 aatgcatcaa gcgcaagccc agttcggcgt cctcgcagat cgtccattcg ctccatgcac
      301 cggtcgcttc cagcgcgccg cggcggacca tcgtcatggt gccgtgctgg atgatcgcgt
      361 tgcgctcgtt gcgatggtgc atgccgatgc ggaagaagcc gtcgtattcc cagttcgtca
      421 tgcgccggaa cgcgttgtgt tcgtagtcgc gatgcgcctg cgggcattgc acgacggcga
      481 ctttcggatc ggcgaagtag ccggtgagcg cacgcagcca gtcggcgcgc acggcgtagt
      541 cggcgtcgat cgcggcaacg acttcggcgc gcgcgtcggt ttccttcaag ccgaaattca
      601 gcgccccggc cttgaagccg ggccacgggt tgagatggaa gaagcggaat ttggggccga
      661 gttttttgca gtgcttctcg accggctgcc agacctcgtc gcgcctcgtg ttgttgtcga
      721 tcaccagcac ttcgaagttt tcgtagtcga gcgcggccag cgagtcgaga gtcaggatca
      781 ccatctccgg cggctcgttg cagcaggcca gatggatcga cacgaacggc tgcgcatcgc
      841 cgggcttcgg ctcgagcagg ccgaaatgac gcagccactt gccgcgccag atcacttcgg
      901 tgaactcgaa accgttgatc agcaggatca ggatgatcgc tacctgcgcc ggcgcgagga
      961 tggtgagcat ggtccagtcg aaaggatcga ggtagaacgc gtaaggcagc gtcgccgacc
     1021 agaccagcag cgacgccgac aactggatca gcacgcagaa gaacgtgagg cccacgacct
     1081 tgaagcgcat gaaatggcgt gcgaaccaga acatcggcaa tagcgcgagc aggcaggcga
     1141 tgccggcctt ccacggccat gccgggtctt cgatcactgg cccggtgaga gaaaatttcg
     1201 gcgtgcggtc cgcgccgaac atgccccagt aggcgccgac gcggccttcg aacggctcct
     1261 tccacggctg gtcgatggct tccatcaggt aatagtcgaa gccgcgttcg cgcgcgacgt
     1321 tgagccactg gcggatgaac aggctttcgt tcgcgatcga ggcggtcgcg cccttgacgc
     1381 ggtcgcccga ggatggccag ccgatttcgc cgatcacgat gtgcttgccc ggaaacgcgc
     1441 tttgcaactg gtagtagcgc atcaacacct gcaggccgat ggcatccttg cgcggatagc
     1501 cttcccagta cggcagcagg tgcacggtga tgaaatccac gtgcttggcg agttgcggat
     1561 atttcagcca gatgtgccag ggttcggcgg tcgacaccgg caccttgacc tgcgcgcgca
     1621 cgcggtcgat gtaggaaatg agggtatcca cgcccatgtc gttgcgcagc agggtttcgt
     1681 tgccgacgat gacgcgggtg acgttgtcgt tggcgcgcgt ttcagcgatc aacgcggcga
     1741 tttcgttctc gtcgttctgc cagcggctgt cgagccaggc gccggccatc acggtcatgc
     1801 cgtatttctt cgcgatcgcc gcaaccgcag gattttccag cgcggcgtaa gtgcgcaggc
     1861 gcttgctgta cttcgacaac agcttgaggt cggcgtcgat ctggtcggtg ttcggataaa
     1921 tctgcttgtt cggattctgg tagcgctggt atggcacgta ggcgaagccg ccgatctcgc
     1981 cgctccagtt ggccggctgc gccggacgat tcagtgctgc ccacaacatg aagttgagag
     2041 cggccacgac gagcgcaagc agcaaggcgc gccacaacga tggacgggtc gcagggttgg
     2101 ggctggggtc caagcaggtt tccaggttcg gaatcggcac gttcggtcgg gtttgcgcgg
     2161 atgggttcac gcaagccggc gcagaatagc ccaaggccgc ggcgcggctc aatcatcccg
     2221 atttgagctt gcgcgcttcc cgccacttgc cgatggcaac cggtaccagc gccagcaggc
     2281 ccatcgcgac cagcggcgcg attaccagcg gatcggaaaa caagctcagg ttgacttcgt
     2341 ggttggtgcc gatgtttttc gccagccccg cgccgagcac ggtttcgatg ccgaccatca
     2401 gcgcgccgcc accggccgtg gcggcgagga acagccacag cggacagcgc agccaggcca
     2461 gcgccacggt gacgccgccg aacgggaaga acggcaccag gcgcaggaaa caggtatagc
     2521 tgaccgggtg cgccagatag ccgccgcgca atttatccac caatgccggc gcgctggcac
     2581 ccgactgcgt gccgaaggcg tggcggctgg cgaggaacag gatcagcgcg cctatcgttg
     2641 cgccggtcgt cgaaaggatg gtgccccaga agacgccgaa cagcatgccg ccggcaagga
     2701 tcaccacgag cacgccgggc acgccggtgg cgatggcaag cgtggtcacg ccgacgtgga
     2761 tcaatccggc gtggatggga tgcgccgcaa tctgcgcctg caggttcgcc tgttcgcggg
     2821 ccaatgtttc cggacgaaaa cggttcagca cgccggtcga aagcagggcg atgccgatgg
     2881 cgatgaggat gagcagtgga aggaacgagc gcaatcgctt cacgacaacc tcggcgggaa
     2941 aagcgaaggc cggcttgcgc cggcctcgcg tggtacccag tttacctgct cgactcagcg
     3001 gcaggtatcg ctctcgaagc cgtcgctgaa gaggcgatcg aaggtgagcg tcagcacttg
     3061 cccatcgtga tcggcgctgc gggccggtgt ggacgaatcg gtaacttcgg gtccggcttc
     3121 cgatgtgtcc gcgttgccgt gcgcattgtg caggctgacg aaatccgtcc atgcggtgcg
     3181 tgtaagcaag gcgtgatcca gt
//
LOCUS       DASRVO010000358         1375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_322634, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000358 DASRVO010000000
VERSION     DASRVO010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322634"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1375
                     /locus_tag="VFF05_04515"
     CDS             <1..>1375
                     /locus_tag="VFF05_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51101.1"
                     /translation="DPDTSDAIYVFRGSASSPAVAVGDIVDVTGPIAEFNGTTEFSGS
                     PLTVTVAGSGGSVTPVLLNGSMPSPDPLVPVCHGTLPAGNVAEGNWECLESMLVSFNG
                     VVVGASSGGFAADATHAGSPGSLYATVDGVARPFREVGAQYPGLGGTIPVWDGNPEIM
                     EIYFGKGYNSIATAPYNAGQTFAMQAVVADFSGAYELYPTVFTPASPPNTVQPVADSA
                     TGTLTIASQNMLHFYDDVNDPGTGDDCTTQGSADTCPTTPQFGIRLAKLSLQVRNVLK
                     APAVVGAQEVENINALQKLADQIHTDDASLTYAPYLVEGNDIGGIDVGFLVRSDVTVN
                     TVTQIGKDTQTDDGCGGTPPCILNDRPPLLLDASFGGQRFAVLVIHNRSLSSVDTLRV
                     QRKRLEQAEYVAAIAQAWQTGTGSDVALVSPDADVTLGANGETVPAADGSVPLVIVGD
                     YNAYEFTD"
BASE COUNT          248 a          461 c          430 g          236 t
ORIGIN      
        1 gatccggata cttccgatgc gatctacgtg tttcgcggca gcgcctcgtc gcctgctgtc
       61 gcggtcggcg acatcgtcga tgtgaccggc ccgattgccg agttcaacgg caccaccgag
      121 ttcagcggca gcccgctgac ggtgacagtg gccggcagtg gcggcagcgt cacgcccgtg
      181 ttgctcaacg gctccatgcc gtctccggat ccgctcgtgc ccgtgtgcca cggcaccttg
      241 cccgccggca atgtcgccga gggcaactgg gaatgcctgg aaagcatgct cgtgagtttc
      301 aacggcgtcg tggtcggagc ctcgagcggc gggttcgccg cagacgccac gcatgcggga
      361 tcgccgggat ctctctacgc cacggtcgat ggcgttgcgc gtccgttccg tgaagtcggc
      421 gcacagtatc cggggctcgg cggcacgatt ccggtctggg acggcaatcc ggaaatcatg
      481 gaaatctatt tcggcaaggg ctacaacagc attgccacgg cgccctacaa cgcgggccag
      541 accttcgcca tgcaggccgt cgtcgccgat ttcagcggcg cctacgagtt gtatccgacc
      601 gtcttcacgc ccgcctcgcc gccgaacacc gtgcagccgg tcgccgattc ggcgacgggt
      661 acgttgacca tcgcctcgca gaacatgctg catttctacg acgacgtgaa tgatcccggc
      721 actggcgacg attgcaccac gcagggttcg gcagacacct gcccgactac tccgcagttc
      781 ggcatccgcc tcgccaaatt gtcgctgcag gtgcgcaacg tgctcaaggc accggccgtg
      841 gtcggcgcgc aggaagtgga aaacatcaat gccttgcaga aactggccga ccagatccac
      901 accgacgacg cgagcctgac ctacgcacct tatctggtcg agggcaacga catcggcggc
      961 atcgacgtgg gcttcctcgt gcgcagcgac gtcaccgtga acaccgtgac ccagatcggc
     1021 aaggacacgc aaaccgacga cggttgcggc ggcacgccac cgtgcattct gaacgatcgc
     1081 ccgccgctgc tgctcgatgc gagcttcggc ggccagcgtt tcgcggtgct tgtcattcac
     1141 aaccgctcgc tgagcagcgt cgacaccctg cgtgtgcagc gcaaacgcct ggaacaagcc
     1201 gaatacgtgg cggcgatcgc gcaggcatgg caaaccggca ccggcagtga tgtggcgctg
     1261 gtttccccgg atgcggacgt gacgctcggt gccaacggcg aaaccgtgcc agcggccgac
     1321 ggcagcgtgc ccctggtcat cgtcggcgat tacaatgcct acgaattcac cgatg
//
LOCUS       DASRVO010000359         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_165690, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000359 DASRVO010000000
VERSION     DASRVO010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165690"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="VFF05_04520"
     CDS             complement(<1..1948)
                     /locus_tag="VFF05_04520"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZW51102.1"
                     /translation="MAIVRTGRRRSLAAFAAFSIGILSLPLAAQTITAPGLSAPGTIY
                     YDALGTPTIKAATSYDVAFLQGYAQAKARFFEMDFDRRAASGTLAALVGHAALANDVQ
                     TRTLGLDRAAFATWQALDADTKGWLQAFSNGVNFWLQTQPLPPEYGALMLTHADPWTA
                     QDSIIIGKGLAFQLSFDLDIQNTIDFGAYSQAAAAAHVDPNALFFGDTHRFAPPDNRF
                     TVPGFTPTDSATTGAPTFASAMATTQASAQQTAAFGNLQVDPRALALAQAYRDKIAEN
                     PFIAPTLHARLGRGGSNEFAVSGAFTATGKPIIANDPHLTLALPPIFMEMHLISGDGL
                     NATGVSVPGAPGIIQGCNSNICWGSTVNPMDVTDTFLETFAVNSYGLPTAIIHNGVQE
                     PVQWVFQSFYVNQMTPGQADRVTRDNSIGYTNGAITVIVPRRNNGPVVQIGTPGSCSL
                     TGQTLNPGLACGLSIAYTGWGATFELKAFKLIDQAQNLSDFQNALTYFDFGSQNFAYA
                     DTAGNIAYFTSAENPIRDDLQNFGKWLGSPPFLIRDGSGQAHNDWLPVSHPQPNQATP
                     FEIMPASEMPHVINPAQGYFANANNDPIGVTAQNNPFGMTRPGGGVYYLNYGYDAYRM
                     GRIDRSLSALVASGKKITTADIQAV"
BASE COUNT          375 a          662 c          766 g          433 t
ORIGIN      
        1 gcacggcctg gatgtcggcc gtggtgatct tcttgcccga agccaccagc gccgacagcg
       61 agcggtcgat gcggcccatg cgataggcgt cgtagccgta gttgaggtag tacacgccgc
      121 cgcccggtcg cgtcatgccg aacggattgt tctgcgcggt gacaccgatg ggatcgttgt
      181 tcgcgttcgc aaagtagcct tgtgccggat tgatcacatg cggcatttcc gaagccggca
      241 tgatctcgaa cggagtcgcc tgattgggct gcggatgcga caccggcagc cagtcgttgt
      301 gcgcctgtcc tgagccgtcg cggatcagga acggcggcga gcccagccac ttgccgaagt
      361 tctgcaagtc gtcgcgaata ggattctcgg cgctggtgaa gtaggcgatg ttgccggccg
      421 tgtcggcgta ggcgaaattc tgcgagccaa aatcgaagta cgtgagcgcg ttctggaaat
      481 cgctcaggtt ctgcgcttgg tcgatcaact tgaacgcttt cagctcgaac gtcgcgcccc
      541 aacccgtgta ggcgatggac agtccgcagg ccaggcccgg attgagcgtc tgcccggtca
      601 gcgaacaact gccgggggta ccgatctgca cgaccggccc attgttccgg cgcggcacga
      661 tcacggtgat cgcgccgttg gtgtagccga tggaattgtc gcgggtgact ctatccgcct
      721 gccccggcgt catctggttg acgtagaaac tctggaatac ccactgcacc ggttcctgca
      781 cgccgttgtg gatgatcgcg gtgggcagac cgtagctgtt gacggcgaag gtttccagga
      841 acgtgtcggt cacgtccatc ggattcaccg tcgaacccca gcagatattg ctgttgcagc
      901 cctggataat gcctggtgcg cccggcacgc tgacgccggt ggcgttgagg ccatcgccgg
      961 aaatcaggtg catctccatg aagatcggcg gcagggccag ggtcaggtgc gggtcgttgg
     1021 cgatgatcgg cttgccggtg gcggtgaacg cgccgctcac cgcgaattcg ttgctgccgc
     1081 cgcggcccag gcgcgcatgc agggtcggcg cgatgaaggg attttccgcg atcttgtcgc
     1141 gataggcctg tgccaacgcc agcgcgcgcg gatcgacctg caggttgccg aacgccgccg
     1201 tttgctgcgc gctcgcctgc gtcgttgcca tcgccgaggc gaacgtcggc gcaccggtgg
     1261 ttgcggaatc ggtcggcgtg aagccgggta cggtgaagcg attgtccggc ggcgcgaaac
     1321 ggtgcgtgtc gccgaagaac agcgcattcg gatcgacgtg cgctgccgcg gctgcctgcg
     1381 agtaggcgcc gaaatcgatg gtgttctgga tgtcgagatc gaacgacaac tggaacgcga
     1441 ggcccttgcc gatgatgatg gagtcctgcg ccgtccacgg atcggcatgg gtcaacatca
     1501 gcgcgccgta ttcgggcggc agtggctgcg tctgtagcca gaaattcacg ccgttggaaa
     1561 aagcctgcag ccagcctttc gtgtcggcat cgagtgcttg ccaggtggcg aacgcagcgc
     1621 ggtcgaggcc gagcgtgcgc gtctgcacgt cgttggcgag cgcggcgtgg ccgaccaacg
     1681 cggcgagcgt gccgctcgcg gcgcggcggt cgaagtccat ctcgaagaag cgcgccttgg
     1741 cctgcgcgta accctgcagg aaggcgacat cgtagctcgt cgccgccttg atcgtcggcg
     1801 tgccgagcgc gtcgtagtaa atcgtgccgg gcgcggacag gccgggcgcc gtgatcgtct
     1861 gcgccgccag cggcagcgaa agtataccaa tggaaaaagc ggcaaaagcc gcaagactcc
     1921 ggcgcctgcc ggtgcgtacg atagccatga tcccctcctg tgggtacgac gcgggcgtcg
     1981 ttcgaacgct cgttcgaggg gaattatgcg ccttgcgtcg actcccgttt gcggcggcgc
     2041 agcaagccgc cgcaaacggt tcgaaacggc tacttgtgcg gcttggccgc ggactgcttt
     2101 tctgccggct ttttctccgg caagggcttg agcatgtcgt ccatgttctt gttcatgtcc
     2161 gtgaacttgt aggcaggcac tgcgaaggtc cagccggaaa aggtcttgtt caggctggcc
     2221 acttccttgt tcaggg
//
LOCUS       DASRVO010000360         1265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_347961, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000360 DASRVO010000000
VERSION     DASRVO010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347961"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1265
                     /locus_tag="VFF05_04525"
     CDS             <1..>1265
                     /locus_tag="VFF05_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZW51103.1"
                     /translation="AAPTLDAPAKRALLWLVAFSFFMQMLDSTIVNTATPSIADSMGI
                     APLDLRTALTSYTLALAVFIPLSSWLADRRGTREIFWWAILVFTLGSLACGLAQNLWM
                     LVAARVVQGFGGAMMMPVGRLALVRAFDKSEFVHAMSVATIPGLIGPAIGPLLGGFFA
                     MYVSWRMIFFVNIPFGIVGLFLARRAMPDYRGARAPLDIAGFALFALGAGGLSWALEQ
                     LSEAVYAQGFVFAIVALAALALYWRLSMRVDAPIVDLRLLRIRSFRVAFNGGFATRLG
                     VGGISFLMTLLFQVGFGYSAVMAGALQIPQALAMLLMRFFVGPILKYQGYRRVLLWNT
                     ALAGILVMTFASMSAATPIWLICVQVFVYGFVMSLQYTAMNTLGFVDLSHAQASMGAS
                     MTSTVQNLSISFGVAFASLLMALYIGDGP"
BASE COUNT          162 a          422 c          403 g          278 t
ORIGIN      
        1 cgccgcaccg acgctggacg cacctgccaa gcgcgccctg ctctggctgg tcgcgttttc
       61 gttcttcatg cagatgctcg actcgaccat cgtcaacacg gcgacgccga gcatcgcgga
      121 cagcatgggc atcgccccgc tcgacctgcg cacggcgctg accagctaca ccctcgcgct
      181 ggcggtgttc atccccttga gttcatggct ggccgaccgc cgcggcacgc gcgagatttt
      241 ctggtgggcg atcctcgtgt tcacgctcgg ctcgctcgcc tgcggcctcg cccagaacct
      301 gtggatgctg gtcgccgctc gcgtcgtgca gggcttcggc ggcgcgatga tgatgccggt
      361 cggacgcctt gcgctggtgc gcgcgttcga caaatccgaa ttcgtgcatg caatgagcgt
      421 ggctacgatt ccggggctca tcggtccggc catcggaccg ctgctcggcg gtttcttcgc
      481 catgtacgtg tcgtggcgga tgatcttctt cgtcaacatt ccgttcggca tcgtcggcct
      541 tttcctcgcg cgccgcgcca tgccggacta ccgcggcgca cgcgcgccgc tggatattgc
      601 cggcttcgcg ttgttcgcgc tcggcgccgg cggattgtcg tgggcgctgg aacaattgag
      661 cgaagccgtc tacgcgcagg gattcgtgtt cgccatcgtt gcgcttgccg cgctcgcgct
      721 gtactggcgc ctgtccatgc gcgtggacgc accgatcgtc gacctgcgcc tgttgcgcat
      781 ccgcagcttc cgcgtcgcct tcaatggcgg tttcgccacg cgcctgggtg tcggcggcat
      841 ttcattcctg atgacgctgt tgttccaggt tggcttcggc tattcggcgg tcatggcagg
      901 cgcgttgcag attccgcagg cactggccat gctgctgatg cgtttcttcg tcgggccgat
      961 cctgaaatac cagggctacc gccgcgtgct gctctggaac acggcgctcg ccggcatcct
     1021 ggtgatgacg ttcgcgagca tgagcgcggc gacgccaatc tggctgatct gcgtgcaggt
     1081 gttcgtctac ggcttcgtga tgtcgctgca atacacggcg atgaacacgc tcggcttcgt
     1141 cgatctttcc cacgcgcagg cgagcatggg cgcgagcatg accagcaccg tgcagaacct
     1201 gtcgatcagt ttcggcgtcg ccttcgcgtc cttgctgatg gccctgtaca tcggcgacgg
     1261 cccgc
//
LOCUS       DASRVO010000361         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_322686, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000361 DASRVO010000000
VERSION     DASRVO010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322686"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFF05_04530"
     CDS             complement(<1..782)
                     /locus_tag="VFF05_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZW51104.1"
                     /translation="MRSLRFFAICALLGGGALASAPALAGSVYRCQGPNGQTAFTNRP
                     GTFTQCHEVASYADKKPTAAEAGKLHSEYHSDSAPTDADTEPVAAVPKAQAADDSAIQ
                     VHRGAVYKVVRANGITEYTNIRPATGATYRVLFTYMATCYACNVHSKLNWGSIPLNLD
                     AYGDEIAAAAKEYRIDPALLRAVIHAESAFKRYAVSDKGAQGLMQLMPGTAADLGVGD
                     PFDAGQNIRGGADYLAQLLKQFNGDEQLATAAYNAGAQNVQK"
     gene            932..2302
                     /gene="miaB"
                     /locus_tag="VFF05_04535"
                     /pseudo
     CDS             932..2302
                     /gene="miaB"
                     /locus_tag="VFF05_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
     gene            complement(2366..2965)
                     /locus_tag="VFF05_04540"
     CDS             complement(2366..2965)
                     /locus_tag="VFF05_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HZW51105.1"
                     /translation="MSANNPGFVRRTWRALRRPSVKWSVLTLLLIGFFGGIIFWGGFN
                     TGMEATNRLEFCISCHEMRDNVYQEYKQTIHYSNRTGVRAICSDCHVPHDWTPKIIRK
                     IQASRELWGKLTGYIDTKEKFESHRMELATREWARMKSVDSRECRNCHSFDAMSADKQ
                     RQSVYTKHMKAKADGQTCIDCHKGIAHHLPKEYRDPDEE"
BASE COUNT          574 a          991 c          953 g          568 t
ORIGIN      
        1 tacttctgca cgttctgcgc gcccgcgtta taggcggccg tggcgagttg ctcgtcgccg
       61 ttgaattgct tgagcaactg cgccaggtaa tcggcgccgc cgcggatgtt ctggcccgcg
      121 tcgaacggat caccaacgcc gagatccgcc gccgtgcccg gcatcaactg catcaggccc
      181 tgcgcgccct tgtcggatac cgcgtagcgc ttgaaggcgg actcggcatg gatcaccgcg
      241 cgcaacagcg ccggatcgat cctgtactcc ttcgccgctg cggcgatctc gtcgccgtag
      301 gcatcgaggt tgagcgggat cgatccccag ttcaatttcg aatgcacgtt gcaggcgtag
      361 caggtcgcca tgtaggtgaa cagcacgcga tacgtcgcac cggtcgccgg ccggatgttg
      421 gtgtactcgg tgatgccatt ggccctgacc accttgtaga cggcgccgcg atgcacctgg
      481 atcgcactgt cgtccgcggc ttgcgctttc ggcacggctg caaccggctc cgtgtcagca
      541 tcggtcggcg ccgaatcgga atggtattcg gaatgcagct tgccggcttc ggcggcggtc
      601 ggcttcttgt cggcgtagct ggccacctcg tggcactgcg tgaacgtacc cggccggttg
      661 gtgaaggcgg tctggccatt cggcccctga cagcggtaca cgcttccggc cagcgcaggc
      721 gcgctcgcca gtgcgccgcc gccaagcagc gcgcaaatgg caaaaaagcg caaactccgc
      781 attgtggaaa aagctggatg catggcagtg ggcccttcga tccgcgcaag tgtccggcag
      841 aacaaggctt ttgccaagtg ccatgccgat tttcgtggcg ccagcgccgg gcgcggctta
      901 aaatgcgcgg tttccagatt cggtccatgc catgtccggc aaattgttca tcaagaccca
      961 cggttgccag atgaacgagt acgactcggc caagatggcc gacgtgctcg ccgcctcgca
     1021 tggcctcgca ctcaccgaaa acgagaccga ggcggacgtc atcctgatca acacctgttc
     1081 gatccgcgaa aaggcgcagg aaaaggtgtt cagccagctc ggccgctgga aggagctcaa
     1141 gcaggccaac ccgcaactgg taatcggcgt cggcggctgc gtggccagcc agtaaggcgc
     1201 ggccatcgtc aagcgtgcgc cgtatgtcga cctcgtgttc ggcccgcaga ccctgcatcg
     1261 cctacccgca atgatcgaag cgcacaaaag cacacaacgc gcacaagtgg acatctcgtt
     1321 ccccgagatc gaaaaattcg accgcctgcc cgagccgcgc gcggaaggcg tcaccgcgtt
     1381 cgtgtcgatc atggaaggct gctcgaaata ctgctcgtat tgcgtcgtgc cgtacacgcg
     1441 cggcgaggag atcagccgtc cgttcgacga cgtcgtcgcc gaagtcgccg cgctcgccga
     1501 ccagggcgtg cgcgaggtga acctgctcgg ccagaacgtc aacgcctatc gcgggccgat
     1561 gcacgacggc gcgtgcgagc ccctcggggc gagcagaaat gagagttatg ctgacctcgc
     1621 cgtcctgatc cacgcgatcg cacaactcga cggcatcggc cgcattcgct tcacgacctc
     1681 gcatccgctg gaattttccg actcgctgat cgaggcctac gcgaacgtgc ccaagctcgc
     1741 caattacctg cacctgcccg tacaagcggg ttcggaccgg atcctgtcgg cgatgaagcg
     1801 cggctacacc acgctcgagt tcaagcagaa gatccgcaag ttgcgcaaag tccgaccgga
     1861 tatttcggtg tcgaccgaca tcatcgtcgg ctttcccggc gaaaccgatg ccgatttcga
     1921 gcagaccatg aaactcgtcg aggacatcgg cttcgaccag agtttttcct tcatcttctc
     1981 gcgccgcccc ggcacgcccg ccgcgaacct ggccgatgat acgccggagt cggtcaagca
     2041 cgggcgcctc gcccgcctgc aagcgacgat caacgcgaac gcgaagcaga tttccgaagc
     2101 catggtcggc agcgtgcaga gcgtgctcgt cgagggcccg tcgaagaaga atccgcacga
     2161 gatgaccggc cgcaccgaaa acatgcgcta cgtcaatttc cccggcgatg cgcgcctgat
     2221 cggccagttc gtcgacgtgc aaatcacgca ggcgatgtcg aactcgctgc gcgggcgcgt
     2281 ggcggccaaa gcggcggcgt gaatcggcgc gaccgtcgcg tagcgccgaa cccgggcgat
     2341 cgcgcctgac cgcggacgac tcgactcact cctcgtccgg atcccggtat tccttcggca
     2401 agtggtgcgc aatgcccttg tggcagtcga tgcaagtctg gccgtcggcc ttggccttca
     2461 tgtgcttggt gtagacgctc tgccgctgct tgtcggcgct catcgcgtcg aaactgtggc
     2521 agttccggca ttcgcgagaa tccaccgatt tcatgcgcgc ccactcgcgc gtggccagtt
     2581 ccatgcgatg tgactcgaac ttttccttgg tatcgatata accggtcagc ttgccccaca
     2641 gctcgcggct cgcctggatc ttgcggatga tcttgggggt ccagtcgtgc ggcacgtggc
     2701 agtcggaaca gatcgcgcgc acgccggtgc ggttcgagta atggatggtc tgcttgtatt
     2761 cctggtagac gttgtcgcgc atctcgtggc agctgatgca gaactccagg cgattggttg
     2821 cctccatgcc ggtgttgaag ccgccccaga agatgatgcc gccgaaaaag ccgatcagca
     2881 gcaaggtcag caccgaccac ttgaccgatg gtcgccgcag cgcccgccac gtcctgcgaa
     2941 cgaacccggg attgttggca ctcatgtggc gatcctttcc ggctcaacgg aacgagaaac
     3001 ggtaggcgac gaaaaccgtg ttgaccgagt agttcggcgc ctgcatgtag gttggcagca
     3061 agctcgtcgg cgtgaagccg tactgg
//
LOCUS       DASRVO010000362         1394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_347978, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000362 DASRVO010000000
VERSION     DASRVO010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347978"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFF05_04545"
     CDS             complement(<1..326)
                     /locus_tag="VFF05_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51106.1"
                     /translation="MANEKYLAVIGLSEEDTAHLRLLLRKVAAELGQNWRWGAEENAD
                     LMIVSPAQLAGQIARNRAFSGGRRCVIFDNDEPLRNGELRLHKPLKAEDVVAALNDTG
                     APSMDLG"
     gene            complement(386..>1394)
                     /locus_tag="VFF05_04550"
     CDS             complement(386..>1394)
                     /locus_tag="VFF05_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW51107.1"
                     /translation="LMAMAAAGVVAHKLSVGDLVLVNTLLLQLSIPLNMLGFTYRQIK
                     QGAIDMERMFALLDANAEVRDAPDAPPLHVAGAQVRFEHVSFAYDPQRQILHDVDFTI
                     APGKTLAVVGTTGAGKSTLSRLLFRFYDASAGRILIDGQDIRSVTQQSLRAAIGIVPQ
                     DTVLFNDSIYYNIAYGRPEATRQEVEAAAKAAHIHDFVMSLPQGYDSQVGERGLKLSG
                     GEKQRVAIARTVLKNPAILVFDEATSALDTRTEKIIQGELAEIARARTTLIIAHRLST
                     IVDADEILVLEHGRAVERGTHAGLLAQGGRYAALWALQQQARRDAAAHSDAEKALETA
                     S"
BASE COUNT          212 a          450 c          462 g          270 t
ORIGIN      
        1 ccgaggtcca tgctcggcgc gccggtgtcg ttgagcgcgg cgacgacatc ctcggccttg
       61 agcggcttgt gcaggcgcaa ttcgccgttg cgcaatggtt cgtcgttgtc gaatatgacg
      121 cagcggcgtc caccgctgaa cgcgcggttg cgcgcgatct gtccggccag ttgcgccgga
      181 ctgacgatca tcagatcggc gttttcttcc gcgccccagc gccaattctg gccgagttcg
      241 gcggcgacct tgcgcagcaa taggcgcagg tgtgcggtgt cctcttcgct caggccgatg
      301 acggcaaggt atttctcgtt cgccacggaa tgcttcctcg accgctcgcg tcgagtttac
      361 ttcaaaacaa cgtggagcgt gcgcttcaag acgccgtttc cagcgctttt tcggcatcgc
      421 tgtgcgccgc cgcgtcgcgc cgcgcctgct gttgcagcgc ccacagcgcg gcatagcgtc
      481 cgccttgcgc gagcagtccg gcgtgcgtgc cgcgctcgac cgcgcggccg tgttcgagca
      541 cgaggatctc gtcggcatcg acgatggtgg aaagccggtg cgcgatgatc agggtcgtgc
      601 gcgcacgcgc gatctccgcc agttcaccct ggatgatttt ctcggtgcgc gtatccagcg
      661 cgctggtcgc ctcgtcgaac acgaggatgg cgggattctt cagcaccgtg cgcgcgatcg
      721 ccacgcgctg cttctcgccg ccggacagtt tcaggccgcg ctcgccgacc tgcgagtcgt
      781 aaccctgcgg cagcgacatc acgaagtcgt ggatgtgtgc ggctttcgcc gccgcttcca
      841 cttcctggcg cgtcgcctcc gggcggccgt aggcgatgtt gtagtaaatg ctgtcgttga
      901 acagcaccgt gtcctgcgga acgatgccga tcgccgcgcg caaggattgc tgcgtcacgc
      961 tgcggatgtc ctgaccgtcg atgaggatgc gcccggcgct ggcgtcgtag aagcggaaca
     1021 gcaggcgcga cagcgtggat ttgcccgcgc cggtggtgcc gaccacggcg agcgtcttgc
     1081 ccggcgcgat ggtgaaatcc acgtcgtgca atatctgccg ctgcggatcg taggcgaagg
     1141 acacatgctc gaaacgcacc tgcgcaccgg cgacatgcag cggcggtgcg tccggcgcgt
     1201 cgcgtacttc ggcattcgca tcgagcaggg cgaacatgcg ctccatgtcg atcgcgccct
     1261 gtttgatctg gcggtaggtg aagccgagca tgttgagcgg gatcgacagt tgcagcagca
     1321 gcgtgttgac caggaccaga tcgccgacgc taagtttgtg cgcgaccacg cccgctgcag
     1381 ccatcgccat cagc
//
LOCUS       DASRVO010000363         1186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_374049, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000363 DASRVO010000000
VERSION     DASRVO010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374049"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFF05_04555"
     CDS             <1..295
                     /locus_tag="VFF05_04555"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005922555.1"
                     /note="catalyzes the conversion of N-acetylornithine to
                     N-acetylcitrulline in an alternative arginine biosynthesis
                     pathway; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HZW51108.1"
                     /translation="NEGYAGAHVVYAKSWGALPYFGRWEAEKPIRDAHKHFIVDEAKM
                     ALTDSGVFSHCLPLRRNVKATDAVMDSPACIAIDEAENRLHVQKAVMAALANQ"
     gene            313..>1186
                     /locus_tag="VFF05_04560"
     CDS             313..>1186
                     /locus_tag="VFF05_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HZW51109.1"
                     /translation="MSKDIVLAFSGGLDTSFCVPYLKERGWAVHTVFADTGGVDAEER
                     AYIEQRAQQLGVASHVTVDGGPALWEHFVKPFVWAGEGYQGQYPLLVSDRYLIVDAAL
                     ARAQALGTRAIAHGCTGMGNDQVRFDLAVKAQGDYAIVAPIREIQKEHTQTRAYEQKY
                     LEERGFGVRAKQKAYTINENLLGVTMSGGEIDRWQAPGEGARGWCKPRAEWPSKPLSV
                     KLTFVAGEAVALDGAKIAGHELLAKLNALFSPYGVGRGLYTGDTTIGLKGRIVYEAPG
                     LTGLLVAHRALEEAV"
BASE COUNT          225 a          379 c          398 g          184 t
ORIGIN      
        1 gaacgagggg tacgcgggcg cgcacgtggt ctacgccaag agctgggggg cgctgcccta
       61 cttcggccgc tgggaggccg agaagcccat ccgcgacgcg cacaagcact tcatcgtcga
      121 cgaagcgaag atggcgctga ccgacagtgg agtgttcagc cactgcctgc cgctgcgacg
      181 caacgtcaag gcgaccgatg cggtgatgga ttcgcctgcg tgcatcgcca tcgacgaagc
      241 cgagaaccgc ctgcacgtac agaaagcggt gatggcggcg ctcgccaacc agtgacccta
      301 cggaatctcc ccatgagcaa agacatcgtc ctcgcgtttt ccggcggact cgacaccagt
      361 ttctgcgtgc cctacctcaa ggaacgcggc tgggccgtgc acacggtgtt cgccgacacc
      421 ggcggcgtgg atgccgaaga acgcgcctac atcgagcagc gcgcgcagca gcttggcgtc
      481 gcctcgcatg tcaccgtcga tggtgggccg gcgttgtggg agcacttcgt caagcctttc
      541 gtgtgggcgg gcgagggtta ccagggccag tatccgctgc tggtgtccga ccgctatctg
      601 atcgtggatg cggcactcgc gcgcgcgcag gcgctaggca cgcgcgcgat cgcgcacggc
      661 tgcaccggca tgggcaatga tcaggtccgc ttcgacctcg cggtgaaggc gcagggcgac
      721 tacgccatcg tcgcgccgat ccgcgaaatc cagaaggaac acacgcagac gcgcgcctac
      781 gagcagaaat atctcgaaga acgcggcttc ggtgtgcgcg caaagcaaaa agcctacacg
      841 atcaacgaga atctgctcgg cgtgaccatg tccggcggcg agatcgatcg ttggcaagcg
      901 ccgggcgagg gcgcgcgcgg ctggtgcaag ccacgcgccg aatggccatc caaacccctg
      961 agcgtgaaat tgacgttcgt cgctggcgag gctgtcgcgc tcgatggcgc gaagatcgcc
     1021 ggccatgaat tgctggcgaa actcaatgcg ctgttttcgc cgtacggcgt cggccgcgga
     1081 ctctacaccg gcgacaccac gatcggactc aagggccgca tcgtgtacga ggcgccgggc
     1141 ctgactgggc ttctcgtcgc gcaccgcgcg ctggaagaag ccgtgc
//
LOCUS       DASRVO010000364         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_193447, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000364 DASRVO010000000
VERSION     DASRVO010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193447"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..691
                     /locus_tag="VFF05_04565"
     CDS             95..691
                     /locus_tag="VFF05_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZW51110.1"
                     /translation="MAIQIAQEQQDGVRILALSGRLDTETAADVELALQDLLGAGERR
                     YLVDLAGIGYVSSAGLRVLLALAKKLDGGKGELKLCDLNASVRQVFDMTGFSKLFAIF
                     PNRDAALGQATKLKPEAEIAQHAARLLGAGSLSSPPHAQADALARAATSVLGLKPMAA
                     AYVSATGMPPVRVVPAPPPALKPAGFLDKLKGWLRKTN"
     gene            complement(688..>2250)
                     /locus_tag="VFF05_04570"
     CDS             complement(688..>2250)
                     /locus_tag="VFF05_04570"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZW51111.1"
                     /translation="GRGQFSEVRLNDPTVSRRHASIRRNGTAWELNDQDSANGTLLRG
                     ARIAGSVPVKDGDELQFGEVKAVFRATPSDHPSLPPPSLPSTNSTRARVAVAATDGAP
                     GAPGLRDLLARLKLFCDIGALSRRSQDLRTTLGEALRALLATFPQASRAAVYARHGGT
                     ETLTRLAQHASATSGGGEFSLTEAFLREALRLEYGIEITDDAGREALAARLRTDTVPA
                     ALLGMPLRMGGETLGALYLESAFAQNAWRPGDRELFAGIAGQLAWLVASQQAQSPERL
                     VEAHDLALARRIQQRFLPQSAPILPGYRFADSYAAARVIGGDYFDYFQYRDGRHGLVI
                     ADVSGKAVSGALYMARLSVQVRVFARNLGGPDELLAGLNRKLAQELEPGMFVTMLAAA
                     IEPESGRLEFANAGHPAPLLRTPDRRVQELGEHGALPLGAMSDTSFPLHTATITPGAS
                     VLFYTDGLDEAHNAAQDLFGKERVIQTLAGSGGGAQGTIDALLAEIARFTDGQAQSDD
                     LTLIMVARERSA"
BASE COUNT          377 a          740 c          767 g          366 t
ORIGIN      
        1 gttgaatcgc atcgcgcgcg aggtcgaacg cccggccatg ggatggccgg gccggttgac
       61 gcaccaggga tggttactcg gcgtggagtt gtgaatggcc atccagatcg cccaggaaca
      121 gcaagacggc gtgcgcatcc tcgcgctgtc cggtcgtctg gatacggaaa ccgcggccga
      181 cgtggaactg gccctgcagg atctgctcgg tgcgggcgag cgccggtacc tggtcgatct
      241 tgccggcatc ggctatgtca gcagcgccgg cttgcgcgtc ctgctcgcac tggccaagaa
      301 actcgacggc ggcaagggcg agctgaaact gtgcgacttg aacgcctcgg tgcgccaggt
      361 gttcgacatg accggcttca gcaagctgtt cgcgatcttc ccgaaccgcg acgccgcgct
      421 gggccaagcg acgaaactca aaccggaagc cgaaatcgcg caacacgcgg cgcgactgct
      481 tggcgccgga tcgctgtcat cgccgccgca tgcacaggcc gacgcgctgg cgcgtgccgc
      541 gacgtcggtg ctcggactca aacccatggc tgccgcctat gtcagcgcta ccgggatgcc
      601 acctgtgcgt gtcgtacccg cgccgccgcc ggcgctcaaa ccggccggtt ttctggacaa
      661 gctcaagggc tggctgcgga aaacgaatta agcgctgcgc tcgcgcgcca ccatgataag
      721 cgtcaggtcg tcgctctggg cctggccgtc ggtgaagcgt gcgatttcgg cgagcagcgc
      781 atcgatcgtg ccttgcgcac cgccaccgct gccggcgaga gtctggatca cgcgctcctt
      841 gccgaacagg tcctgcgcgg cgttgtgcgc ctcgtccaga ccgtcggtat agaacaggac
      901 gctggcgccg ggcgttatgg tcgccgtgtg cagcggaaaa ctcgtatccg acatcgctcc
      961 gagcggtagc gcaccatgct cgccgagttc ttgcacgcgc cggtcgggtg tgcgcagcaa
     1021 tggcgcgggg tgtccggcat tggcgaattc caggcggccg gactcgggtt cgatcgccgc
     1081 tgccagcatg gtcacgaaca tgcccggctc gagttcctgg gcaagcttgc ggttgagtcc
     1141 cgcgagcagt tcgtccggac cgccgaggtt gcgtgcaaac acccgcacct gcaccgacaa
     1201 gcgcgccatg tacaacgcgc cggacacggc cttgccggaa acgtcggcga tgaccaggcc
     1261 atggcgcccg tcgcggtatt ggaaataatc gaaatagtcg ccgccgatca cgcgcgcggc
     1321 ggcgtagctg tcggcgaaac gatagcccgg caggatcggc gcggattgcg gcaggaagcg
     1381 ttgctggatg cgccgcgcga gcgccagatc gtgcgcttcg accaggcgtt cgggcgactg
     1441 tgcctgctga ctggcgacga gccaggccag ttgcccggcg atgccggcga acaattcgcg
     1501 atcgccgggt cgccaggcgt tttgcgcgaa cgcgctttcc agatacagcg cgccgagcgt
     1561 ttctccgccc atccgcaacg gcatgccgag cagggcggcg ggcacggtat ccgtgcgcag
     1621 gcgcgcggcc agcgcttcgc ggcccgcgtc atcggtgatt tcgatgccgt attccagacg
     1681 cagcgcttcg cgcaagaacg cttcggtcag ggagaattcg ccgccgccgg aggtcgcact
     1741 ggcatgttgc gccaggcgcg tcagcgtttc cgtgccgccg tgacgggcat agaccgccgc
     1801 gcggctggcc tgcggaaaag tggcgagcag cgcgcgcaat gcttcgccga gcgtcgtgcg
     1861 cagatcctgc gagcgccgcg acagcgcgcc gatgtcgcaa aacaatttca ggcgcgcgag
     1921 caaatctcgc aatcctggtg cgcccggcgc gccatcggtg gcggcgacag ccacgcgtgc
     1981 gcgcgtcgag ttcgtgctcg gcagactggg cggcggcaac gaagggtgat cgctcggcgt
     2041 ggcgcggaaa accgccttga cctcgccgaa ctgcaactcg tcgccgtcct tgaccggcac
     2101 cgatccggca atccgcgctc cgcgcagcaa agtaccgttg gcgctgtcct gatcgttcaa
     2161 ttcccaagcc gtgccattgc gacggatcga tgcgtggcgg cgcgaaacgg tcggatcgtt
     2221 caggcgtact tcggaaaact gaccgcgacc
//
LOCUS       DASRVO010000365         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_297526, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000365 DASRVO010000000
VERSION     DASRVO010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297526"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFF05_04575"
     CDS             <1..221
                     /locus_tag="VFF05_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZW51112.1"
                     /translation="MEIPGYARRFDAARVPTALVHGWSDALCPVDAAIAFARAREDEI
                     VLVRDDHRLSSHVDFVAERFRDFLGKFA"
     gene            218..2341
                     /gene="rlmKL"
                     /locus_tag="VFF05_04580"
     CDS             218..2341
                     /gene="rlmKL"
                     /locus_tag="VFF05_04580"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HZW51113.1"
                     /translation="MKEFFATCPKGLEYLLVDELKALGAADVHEALAGVHFGGELEVG
                     YRACLWSRLASRILMPLAQFDVPDADALYAGARAIDWSAHMDIATSFAVDAVGSVQGL
                     THSQYAALRVKDAIVDAFRDATGERPNVDTENPGLRVNLVLRRGRAIIGINLSGPPLH
                     QRGYRKGAGLAPLKENLAAAMLLRAGWPQVYAAGGGLVDPLCGSGTLPIEAALMAADV
                     APGLRRAHWGFLGWRGHDAALWKTLHDVAQARAQTGLQALRPVFFGFDENPAVLNEAK
                     RNAQEAQLSGFIQLGRQTLEHLHRPHELEQPGLLITNPPYGERMGVDADVIALYRQLG
                     AKLKTEFRGWRAAVIAADEAHGHALGLRADKRYKLYNGAIECELLNFDIATQDAPRPE
                     PKPLSAGAQMVKNRIEKNFRHLRKIAARENIECWRVYDADLPEYAAAIDIYGEHLHIQ
                     EYQAPASVDENLARTRWRELVRVAGEALGIPRERIALKQRRRGKGGEKYGRLDERGEF
                     LEVNESGLKFLVNLHDYLDTGLFLDHRPLRARVRELARGKRFLNLFCYTGSASVYAAA
                     GGAAETVSVDLSQTYLDWAARNFALNDLDAATNRLVRDDAATYLQSHSAMFDLIFVDP
                     PTFSNSKRAEDFDVQRDHARLLNLCADRLLPSGTILFSNNFRRFKLDEAGLARFTIGE
                     ISAQTIPFDFTRHARIHRAWELRLK"
BASE COUNT          427 a          770 c          813 g          429 t
ORIGIN      
        1 cgatggagat tcccggctac gcgcggcgct tcgatgcggc gcgcgtgccg accgcgctgg
       61 tgcatggctg gagcgacgcc ttgtgtccgg tggacgcggc gatcgcgttc gcgcgggcgc
      121 gcgaggacga gatcgtgctg gtgcgcgacg atcatcggtt gtcgagccac gtcgatttcg
      181 tcgccgagcg atttcgcgat tttctcggta aatttgcgtg aaagaattct tcgccacttg
      241 cccaaagggc ctggaatacc tgctcgtcga cgagttgaaa gcgctgggcg cggcggacgt
      301 gcacgaggcg ctggccggcg tgcatttcgg cggagaattg gaggtcggtt atcgcgcctg
      361 cctgtggtcg cgcctggcga gccggatatt gatgccgctt gcgcagttcg acgtgcccga
      421 tgcggatgcg ctctacgccg gcgcgcgcgc catcgactgg tccgcgcaca tggacatcgc
      481 cacgagcttt gcggtggacg cggtcggcag cgtgcaaggc ctgacccaca gccagtacgc
      541 ggcgttgcgg gtgaaggatg cgatcgtcga tgcattccgg gatgcgaccg gtgaacgccc
      601 gaacgtggat acggaaaatc ctggtctgcg cgtgaatctg gtcttgcgcc gtggccgcgc
      661 catcatcggc atcaatctct ccggtccgcc gctgcaccag cgcggctacc gcaagggcgc
      721 ggggctggcg ccgctgaagg aaaacctcgc cgcggcgatg ctgctgcggg ccggctggcc
      781 gcaggtttac gcggctggcg gcggtctggt cgacccgctg tgcggctcgg gcacgctgcc
      841 gatcgaagcg gcattgatgg cggcggatgt cgcacccgga ctgcgccgcg cgcattgggg
      901 atttctcggc tggcgcggtc acgatgcagc attgtggaaa acgctgcacg acgtcgcgca
      961 ggcacgcgca cagacaggct tgcaggcgct gcgcccggtg ttcttcggct tcgacgagaa
     1021 cccggccgtg ctcaacgagg ccaaacgcaa cgcccaggaa gcgcagcttt ccgggttcat
     1081 tcaactcggg cgacagacgc tggaacatct gcatcgtccg cacgaactgg aacagccggg
     1141 cctgctcatc accaatccgc cctacggcga acgcatgggc gtggacgcgg atgtcatcgc
     1201 gctatatcgc cagctcggcg cgaaactgaa aaccgaattt cgcggctggc gcgccgccgt
     1261 catcgccgcg gacgaggcac acggtcatgc gctcggcctg cgcgccgaca agcgctacaa
     1321 gctgtacaac ggcgcgatcg aatgcgagct gctgaacttt gacatcgcca cgcaggatgc
     1381 gccacgaccg gaaccgaagc cgctttcggc tggcgcgcag atggtgaaga accgcatcga
     1441 gaagaatttc cgccatctgc gcaagatcgc cgcgcgtgaa aatatcgaat gctggcgcgt
     1501 gtacgacgcc gatctgcccg aatacgccgc tgccatcgac atctacggcg agcatctgca
     1561 catccaggaa taccaggcgc cggccagcgt ggacgagaac ctcgcgcgca cgcgctggcg
     1621 cgagttggtg cgcgtagccg gcgaggcgct gggcattccg cgcgaacgca tcgcgctcaa
     1681 gcaacgccgg cgcggcaagg gcggcgaaaa gtacggacgc ctcgacgagc gcggcgaatt
     1741 cctggaggtg aacgagagcg gcctgaaatt tctcgtcaac ctgcacgatt atctcgacac
     1801 cggattgttc ctcgatcacc gcccgttgcg cgcgcgcgtg cgcgagctgg cgcgcggcaa
     1861 gcgtttcctg aatctgttct gctacaccgg cagcgcgagc gtttacgccg ccgcgggcgg
     1921 cgcggcggaa acggtgagtg tggatctgtc gcagacgtat ctggattggg ccgcgcgcaa
     1981 tttcgcgctc aacgacctcg atgctgcgac caatcgcctg gtccgcgacg atgcggcgac
     2041 gtacctgcaa tcgcattcgg cgatgttcga cctgatcttc gtcgatccgc cgacgttctc
     2101 caattccaag cgcgccgagg acttcgacgt gcagcgcgat cacgcgcggc tgctgaactt
     2161 gtgcgcggat cgcctgttgc ccagcggcac gatcctgttt tcgaacaatt tccggcgttt
     2221 caagctcgat gaagctgggc tcgcgcggtt cacgatcggc gaaatcagcg cacagacgat
     2281 tcccttcgat ttcacgcgcc acgcacgcat tcaccgtgcg tgggaactgc gcctgaagtg
     2341 atcgcgttca ggttcgccgg agcgaagctg aacaagccac gccgtggcgg gcgaatttca
     2401 gtttcgattc agccgtgcgg atcgaccatg cgcctgcga
//
LOCUS       DASRVO010000366         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_297536, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000366 DASRVO010000000
VERSION     DASRVO010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297536"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..796)
                     /gene="lolA"
                     /locus_tag="VFF05_04585"
     CDS             complement(146..796)
                     /gene="lolA"
                     /locus_tag="VFF05_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HZW51114.1"
                     /translation="MRIFVLILLLLTTTLARAADTARARLDAFANGIKTISGDFAQQV
                     FDPNGGTGKASSGTLALKAPRQFRWDVAKPYQQLIVADGEKVWVYDPDLEQVTVRAQG
                     TEEAHSPLTVLTDLSQLDRDFSTSEQGMHDGMTWLRLKSKEKEPQFAWCDLGFDVNGL
                     VRMDFEDSLGNRTTITFSHWQRNPKLPVGTFHFVPPKGVDVIGDATPAAQAFPVRN"
     gene            complement(858..>1968)
                     /locus_tag="VFF05_04590"
     CDS             complement(858..>1968)
                     /locus_tag="VFF05_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HZW51115.1"
                     /translation="GNPVVADLAKMPHVLVAGTTGSGKSVGLNAMILSLLYKAEARDV
                     RLLMIDPKMLEMSVYEGIPHLLAPVVTDMKEAANALRWCVAEMERRYKLMAAVGVRNL
                     AGFNKKVKDAIDSGKPLSDPLFKAPEDTTQPIVVPTLEALPYVVVIIDEFADMMMIVG
                     KKVEELIARLAQKARAAGVHLILATQRPSVDVITGLIKANIPTRIAFQVSSKIDSRTI
                     LDQSGAETLLGHGDMLYLPPGTATPERVHGAFVGDDEVHKVVAHLRNQGKPDYIENVF
                     AEVQSLGDGRVVGESGLPEDAEEGEGDYDELYDKAVAIVTESRRASISGVQRRLKIGY
                     NRAARLIERMEADGIVSPPEHNGNREVLAPPPPEI"
BASE COUNT          335 a          642 c          626 g          365 t
ORIGIN      
        1 ggagcgtgcg gcggcgtgtt cggcgaaagc tacgcgagcc tcggcgccgt cgccaacgtg
       61 atcccggtcg atgtcgcgat tcccggatgt ccgccgacgc cgaccgcgct gctgcagggc
      121 atactcgcgg cgatcggcaa acgcgctagt tcctgaccgg aaacgcctgc gccgcgggcg
      181 tggcatcgcc gatcacgtcc acacccttgg gcggcacgaa atggaatgtg ccaaccggca
      241 gcttcggatt gcgctgccag tgcgaaaacg tgatcgtcgt gcgattgccg agggaatcct
      301 cgaaatccat gcgcacgagt ccgttcacat cgaaaccaag atcgcaccat gcgaactgcg
      361 gttccttctc ctttgatttc aaccgcagcc aggtcattcc atcgtgcatg ccctgctcgc
      421 tggtcgaaaa atcgcgatcc agttgcgaca ggtcggtcag gaccgtgagc ggactgtgcg
      481 cctcctcggt gccctgcgcg cgcacggtga cttgttccag gtccggatcg taaacccaca
      541 ctttctcgcc atcggcgacg atgagctgct gatacggctt ggccacatcc cagcggaact
      601 ggcgcggcgc cttgagcgcg agcgtgccgg agctggcctt gcccgtgccg ccgttcggat
      661 cgaatacctg ctgggcaaaa tcgccggaaa tcgtcttgat gccgttggcg aaggcgtcca
      721 agcgcgcgcg cgcggtgtcg gcagcacggg caagtgtggt ggtcaacaaa agcaggatca
      781 aaacgaaaat acgcatggca ggcaactcgg gtttcgcatt ggcgatattg cggcgtccac
      841 gctgaacgcg cgcgtagtca gatttccggc ggtggcggcg caagcacctc gcggttgccg
      901 ttgtgctcgg gcgggctgac gataccatcg gcttccatgc gctcgatcag gcgcgcggcg
      961 cggttgtagc cgatcttcag gcgccgctgc acgccggaaa tcgaggcgcg gcgtgattcc
     1021 gtgacgatgg cgacagcctt gtcgtacagc tcgtcatagt cgccctcgcc ttcttccgcg
     1081 tcctcgggca gtccggactc gccgacgacg cgaccatcgc cgagcgactg aacttcggca
     1141 aacacattct cgatgtaatc aggcttgccc tggttgcgca gatgcgccac aaccttgtgc
     1201 acttcgtcgt cgccgacgaa ggcgccgtgc acgcgctcgg gcgttgccgt gcccggcggc
     1261 agatacagca tgtcgccgtg accgagcagg gtttcggcgc cggactggtc caggatcgtg
     1321 cgcgagtcga tcttgctcga cacctggaac gcgatgcggg tggggatgtt ggccttgatc
     1381 aggccggtga tcacgtcgac cgacggccgc tgcgtcgcca ggatcagatg cacgccggcg
     1441 gcgcgcgcct tctgcgccag ccgcgcgatc agttcctcga ccttcttgcc gacgatcatc
     1501 atcatgtcgg cgaattcatc gatgatcacg accacgtaag gcagggcttc cagcgtcggc
     1561 accacgatcg gctgcgtcgt gtcctccggc gccttgaaca gcggatccga caagggcttg
     1621 ccgctgtcga tggcgtcctt caccttcttg ttgaagccgg cgaggttgcg cacgccgaca
     1681 gcagccatca gcttgtagcg gcgctccatc tcggccacgc accagcgcag cgcgttggcg
     1741 gcctccttca tgtcggtgac gaccggcgcg agcaggtgcg ggatgccctc gtacaccgac
     1801 atctccagca tcttcgggtc gatcatgagc agccgcacgt cgcgcgcttc ggccttgtac
     1861 agcagcgaca ggatcatcgc gttcagcccg accgacttgc cagagccggt ggtgcccgcc
     1921 accaatacgt gcggcatctt ggccaggtcg gcgaccacgg gattgccg
//
LOCUS       DASRVO010000367         1397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_270891, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000367 DASRVO010000000
VERSION     DASRVO010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270891"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1039)
                     /locus_tag="VFF05_04595"
     CDS             complement(107..1039)
                     /locus_tag="VFF05_04595"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZW51116.1"
                     /translation="MKRRIVVALAAALTATAGIASAADATKHPTMGEVLAAAKPSDWH
                     ALDPANTLYLQLASGRVIIELAPQFAPQTVANIKTLVREKYFDGLDILRVQDNYVAQW
                     GDPNADDAKLRRSLGKAKAHVPAEFMVPLAKDFTFTPLPDRDGWAPQAGFVGDFPAAV
                     DLQAHVAWPTHCYGTVGVGRDNAPDSGNGAELYATIASSARWLDRNITVAGRVVQGME
                     LLSSLPRGHGDLGFYTKSEHPVLIKTVRLAVDVPEKDRAHLEVLRTDTPTFAALVESR
                     RNRQDEWYKRPAGYIDVCSVPLPVRAVSGATHAR"
     gene            complement(1036..>1397)
                     /locus_tag="VFF05_04600"
     CDS             complement(1036..>1397)
                     /locus_tag="VFF05_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HZW51117.1"
                     /translation="AKSLHTSTQAFDAAYAARAASGFKLSAKQSARIDELMGRMEQAL
                     TDPNGLPGREWFKHMIYAPGLLTGYGVKTVPGVREALEARNWQQADQYAAITAKVLDG
                     YRAELDKLTALLQANAR"
BASE COUNT          231 a          460 c          452 g          254 t
ORIGIN      
        1 gctctgaacg tgtttcttgg tgacttcttt gcacgagcaa agaagttacc cgggcggcgc
       61 agccgcacgg aagctgtgct ttgacggaag cttcgcacta agagagtcac ctcgcgtgcg
      121 tagcaccgct aaccgcgcgc accggcagcg gcacactgca cacatcaata tatccagccg
      181 gccgtttgta ccactcatcc tgccgattcc ggcgcgattc gaccagcgcc gcgaacgtgg
      241 gcgtatccgt gcgcaggact tccagatgcg cacgatcctt ctccggcaca tccaccgcca
      301 gccgaacggt cttgatcaat actggatgtt cggacttcgt gtagaagccc aggtcgccat
      361 gcccgcgcgg caggctggac agcaattcca tgccctgcac cacgcgcccg gcgacggtga
      421 tgttgcgatc cagccagcgc gcggacgagg cgatcgtggc gtacaactcg gcgccgttgc
      481 ccgagtcggg agcgttgtcg cgtccgacgc cgaccgttcc gtagcaatgc gtcggccacg
      541 cgacgtgcgc ctgcaaatcc acggcggcgg gaaaatcgcc gacgaagccg gcctgcggcg
      601 cccagccgtc gcggtcgggc aagggcgtga aggtgaaatc cttcgccagc ggaaccatga
      661 attccgccgg cacgtgcgct ttggccttgc cgagcgaacg gcgcagcttc gcatcgtcgg
      721 cgttcggatc gccccactgc gcgacgtagt tgtcctgcac gcgcaagatg tcgaggccgt
      781 cgaagtattt ctcgcgcacc agagtcttga tgttggcgac ggtctgcggc gcgaattgcg
      841 gcgcgagttc gatgatcacg cgcccggacg cgagttgcag gtacagcgtg ttggccggat
      901 cgagcgcgtg ccagtcggac ggtttcgctg ccgccaggac ctcgcccatg gtcggatgct
      961 ttgtcgcatc ggcggcggag gcgatgccgg cagtcgcggt gagcgccgcc gccagtgcaa
     1021 cgacgatgcg ccgcttcatc gtgcgttcgc ctgcagcagc gcggtgagct tgtcgagctc
     1081 ggcgcggtaa ccgtccagca ccttggccgt gatcgccgcg tactgatcgg cctgctgcca
     1141 gttgcgcgct tcgagcgcct cgcgcacgcc cggcacggtt ttgacgccgt aaccggtgag
     1201 caggcccggt gcatagatca tgtgcttgaa ccactcgcgt cccggcaggc cgttcggatc
     1261 ggtcagggcc tgctccatgc gacccatcaa ctcatcgatg cgcgccgatt gtttcgcgga
     1321 caacttgaaa ccactcgccg cgcgtgcggc gtaagcggca tcgaaggcct gcgtgctcgt
     1381 gtgcagactc ttggccg
//
LOCUS       DASRVO010000368         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_270902, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000368 DASRVO010000000
VERSION     DASRVO010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270902"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..625)
                     /locus_tag="VFF05_04605"
     CDS             complement(53..625)
                     /locus_tag="VFF05_04605"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZW51118.1"
                     /translation="MRIVFFGPNGSGKGTQAALLKAELKVPHISTGDLLRAAVKAGTP
                     LGLKAKAVMDAGKLVSDEIVLGMLEERVAESDARGGFILDGYPRNLAQCAALEDLLAR
                     IRQPLDIAIELDVPDSVILARCEIRFKAEHRPDDDPAIVRKRLEVYREQTAPVARHFA
                     DLGKLVTVNGVGAVDEVFTRILAVLPRAVA"
     gene            721..1986
                     /locus_tag="VFF05_04610"
     CDS             721..1986
                     /locus_tag="VFF05_04610"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZW51119.1"
                     /translation="MSKGNLLYAQSGGVTAVINATACAVIQACRRRRVGVFAARNGII
                     GALREELFDLSNLDAATVAALRHTPGGAFGSCRFKLKSPDADRAHYERLIAVLRAHDI
                     RHFLYNGGNDSADTSLKIAQIAQTLGYPLNVIGVPKTVDNDLPVTDSCPGFGSVAKYT
                     AVSVLEASLDVASMHESSTKVFVMEVMGRHAGWIAAAAGLAGKRADEPPHVILFPEIP
                     FDEAAFLARVKATVARVGWCTVVVSEGVKNADGKFLAEAGTTDAFGHTQLGGVGPMIA
                     QLVKSKLGYKFHWAVPDYLQRSARHMASKVDAEQAHAVGKAAVEYALAGMNGVMPVIK
                     RVSGTPFKWKLVPAPLTAIANVEKKLPRGFISADGFGITPAARRYLEPLIRGEDAPPY
                     DAVSGLPKYAKPGLKLAKKKLPDYALADK"
     gene            2104..>3408
                     /locus_tag="VFF05_04615"
     CDS             2104..>3408
                     /locus_tag="VFF05_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011038412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HZW51120.1"
                     /translation="MFFQQYAIWIALGCALLAILYGIWSRAWILAKPAGNERMQAIAQ
                     AVQEGASAYLSRQYRTIAIVGVVLFLVIGFTKLGWTAAIGFAIGAFLSGLTGFIGMNI
                     SVRANVRTAEAARNGINAALQIAFRGGAITGMLVVGLALLGVAGYYAILLHFGHSQEE
                     ALHAMVGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDCAGMAADLFETYAVTLIATMLLGGVLGKDVGDYGVLYPLALGGVSIIASII
                     GTFFVKARPGGKIMNALYMGVGVSAALALLAFIPVTQAMMAASKWGVWNIYGCAVIGI
                     LLTVAMVVITEYYTATEYAPVRHVAQASTTGHATNIIAGIGVSMKATAFPVLAICAAI
                     WGAHFCAGLYGIAIAATAMLSMTGMIVALDAYGPITDNAGGIA"
BASE COUNT          596 a         1143 c         1082 g          587 t
ORIGIN      
        1 caacacgaac agaaacgctg gcatgggagt cgtcgccgtt atggccgcgc gctcaggcca
       61 cggcgcgcgg cagcacggcg aggatgcgcg tgaacacctc gtcgacagcc ccgaccccat
      121 tgactgtgac gagcttgccc aagtcggcga aatgccgcgc caccggcgcg gtttgctcgc
      181 gatagacttc caggcgcttg cgcacgatcg ccggatcgtc gtccgggcga tgttctgcct
      241 tgaagcggat ttcgcagcgc gcgaggatca ccgaatccgg cacgtccagt tcgatggcga
      301 tgtccagcgg ctggcgaatg cgcgccagca aatcctccag cgcggcgcac tgggcgagat
      361 ttcgcggata gccgtcgagg atgaacccgc cgcgcgcgtc ggattcggcg acgcgttctt
      421 cgagcatgcc gagcacgatt tcgtcagaca cgagcttgcc cgcgtccatc accgccttgg
      481 ccttgagccc aagcggcgtg ccggccttga ccgccgcacg cagcaggtcg ccggtggaga
      541 tgtgcgggac cttcaattcg gccttgagca acgcggcctg ggtgcccttg cccgaaccgt
      601 tcgggccgaa gaaaacgatg cgcatgtgtc tggatttatt ctgcttgcgg tgggcaagag
      661 ccaccccgac gggctaacct agcggcgttg tcacacttag tcaaacggtg aaatcaagcc
      721 atgagcaaag gaaacctcct gtacgcccag tccggcggcg tcaccgccgt gatcaacgcg
      781 acggcctgtg cggtgatcca ggcctgccgg cggcgcaggg tcggcgtatt cgccgcacgc
      841 aacggcatca tcggcgcgct acgcgaggag ttgttcgacc tgtcgaacct cgatgccgcg
      901 accgttgccg cactgcggca tacgcccggt ggcgcgttcg gctcctgccg cttcaagctg
      961 aaatcgccgg atgcggaccg cgcgcattac gaacgcctga tcgcggtact gcgcgcgcac
     1021 gacatccgcc atttcctcta caacggcggc aacgattcgg ccgatacctc gctgaagatc
     1081 gcgcagatcg cgcaaacgct gggctacccg ctcaatgtga tcggcgtgcc gaaaacggtg
     1141 gacaacgacc tgccggtcac cgattcctgc cccggcttcg gttcggtcgc caaatacacc
     1201 gcggtgtcgg tgctcgaagc gagtctggat gtggcttcca tgcacgaatc ctcgaccaag
     1261 gtattcgtga tggaagtaat gggccgccac gccggctgga tcgcggccgc ggcagggctc
     1321 gccggcaagc gcgcggacga acccccgcac gtgatcctgt ttccggaaat ccccttcgac
     1381 gaagccgcat tcctcgcgcg cgtgaaggcc acggtcgcgc gcgtgggctg gtgcaccgtc
     1441 gtcgtctccg aaggcgtgaa gaacgcggac gggaaattcc tcgccgaagc cggcaccacc
     1501 gatgccttcg gccacaccca gctcggcggc gtggggccaa tgatcgcgca gctcgtgaaa
     1561 agcaagctcg gctacaaatt ccattgggcc gtaccggatt acctgcagcg ttcagcgcgg
     1621 cacatggcga gcaaggtcga cgcggaacag gcccatgcgg tcggcaaggc ggcggtcgaa
     1681 tacgcactcg ccggcatgaa cggcgtcatg cccgtgatca aacgcgtatc cggcacaccg
     1741 ttcaagtgga agctcgtacc cgcaccgctc accgccatcg ccaacgtcga gaagaaactg
     1801 ccgcgcggtt tcatcagcgc ggacggattt ggcatcacgc ccgccgcgcg gcgctatctg
     1861 gaaccgctga tccgcggcga ggatgcaccg ccctacgacg ccgtgagcgg gctgccgaag
     1921 tatgcaaaac ccggactgaa gctggcgaag aagaaattgc cggactatgc gctggcagac
     1981 aaatagcgtc gctttgttgc gccgcaagag tggcgcggcc gcgcgctgcc gttatactcg
     2041 acaggatttt tcgttgcacc ccccgccata ccgttcaatg cctgagattc gagggagaat
     2101 ccgatgtttt tccagcaata cgccatctgg atcgcgctgg gctgtgccct gctcgcgatc
     2161 ctttacggaa tctggtcgcg cgcgtggatc ctggccaaac cggccggcaa cgaacgcatg
     2221 caggccatcg cgcaggccgt gcaggaaggc gccagcgcct acctgagccg ccagtaccgc
     2281 accatcgcca tcgtcggcgt ggtgttgttt ctcgtcatcg gcttcaccaa gctcggctgg
     2341 accgccgcga taggcttcgc catcggcgcg ttcctgtccg gcctcaccgg cttcatcggc
     2401 atgaacatct cggtacgcgc caacgtgcgc acggccgaag ccgcacgcaa cggcatcaac
     2461 gcggcgttgc aaatcgcctt ccgcggcggc gcgatcaccg gcatgctggt ggtgggcctg
     2521 gccctgctcg gcgtggccgg ctactacgcg atcctgctgc acttcgggca tagccaggaa
     2581 gaagccctgc acgccatggt cggccttgcc ttcggctcgt cgctgatctc gatcttcgcg
     2641 cgcctgggcg gcggcatctt caccaagggc gcggacgtgg gcgccgatct ggtcggcaag
     2701 gtcgaagccg gcattcccga agacgacccg cgcaatccgg ccgtgatcgc ggacaacgtg
     2761 ggcgacaacg tcggcgattg cgccggcatg gccgccgacc tgttcgagac ctatgccgtg
     2821 accctgatcg ccaccatgct gctcggcggc gtgctcggca aagacgtggg cgactacggc
     2881 gtgctgtatc cgctcgcgct cggcggtgtt tccatcatcg cctcgatcat cggcacgttc
     2941 ttcgtcaagg cccgccccgg cggcaagatc atgaacgcgc tgtacatggg cgtcggtgtt
     3001 tccgcggcgc tcgccctgct cgccttcatc ccggtcacgc aggcgatgat ggccgcttcc
     3061 aaatggggcg tgtggaacat ctacggctgc gcggtgatcg gcatcctgct cacggtggcg
     3121 atggtcgtca tcaccgagta ctacaccgcg accgagtacg cgccggtgcg ccacgtcgcg
     3181 caggcttcga ccaccggcca tgcgaccaac atcatcgccg gcatcggcgt gtcgatgaaa
     3241 gcgacggcat tcccggtgct ggcgatctgc gccgcgatct ggggcgcgca cttctgcgcc
     3301 ggcctgtacg gcatcgcgat cgccgccacg gcgatgttgt cgatgaccgg catgatcgtc
     3361 gcgctggatg cgtacggccc gatcaccgac aacgccggcg gcattgcc
//
LOCUS       DASRVO010000369         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_297570, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000369 DASRVO010000000
VERSION     DASRVO010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297570"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFF05_04620"
     CDS             <1..583
                     /locus_tag="VFF05_04620"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZW51121.1"
                     /translation="NNQVLQRLAFHDPLTGLPNRAMFEPALAATLNECHDNGTKAALF
                     YLDIDSFKSINDTFGHDVGDQLLTTLADRLRVWMPERATAARLGGDEFVLLIAPLAEA
                     AEHIESATVKLVHALRATLERPLRTKSCVIHPGVSIGVAVYPDSAAGAEALLQSADKS
                     MYQMKGLHYQAASVTRWTEHGDAGAIARNPASH"
     gene            585..1154
                     /locus_tag="VFF05_04625"
     CDS             585..1154
                     /locus_tag="VFF05_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZW51122.1"
                     /translation="MAAASISASGARSVAVNGYKGLHAPRAVLRMALPLVAIVLGACQ
                     APPVKPASVDQRATVLSALGFVSGDDGWLLNLPEPISFELDKDTLKPSMQESIATTAA
                     GLLKANVHKLRIEGHTDDSGPRDYNVALSQRRAETVAQEFISHGFARTDVVEVGMGPD
                     HPLQPNDTRKNRAANRCVVIIVPAEALNQ"
BASE COUNT          209 a          411 c          378 g          183 t
ORIGIN      
        1 caacaaccag gtgctgcagc gcctcgcctt ccacgatccg ctcaccggcc tgcccaaccg
       61 cgccatgttc gaacctgcgc tggccgcgac cttgaacgag tgccacgaca acggcacgaa
      121 agcggcgctg ttctatctcg atatcgattc gttcaagtcg atcaacgaca cgttcggcca
      181 cgacgtcggc gatcagttgc tgaccacgct cgccgaccgg ctgcgcgtgt ggatgcccga
      241 gcgcgccacg gcggcgcgcc tgggcggaga cgagttcgtg ctgctgatcg ccccgctggc
      301 tgaagccgcc gagcacatcg aatcggcgac ggtgaagctc gtgcacgcgc tgcgcgcgac
      361 gttggagcgg cccctgcgca ccaagagttg cgttatccat ccgggcgtga gcatcggtgt
      421 ggcggtgtat ccggacagcg ccgccggcgc cgaggcgctg ctgcaatccg ccgacaagtc
      481 gatgtaccag atgaaagggc tgcattacca ggcggcgtcg gtcacgcgct ggaccgagca
      541 cggcgatgcc ggtgcgattg cgcgcaaccc ggcgtcgcac tagcatggcc gccgcctcca
      601 tcagcgcgag cggtgcccga tctgtggccg tcaacggata caaggggttg catgcgccac
      661 gggccgtgct gcgcatggcg ctgccgctcg tcgccatcgt cctcggggcc tgccaggcgc
      721 cgccggtcaa gcctgccagc gtcgaccagc gcgccaccgt gctcagtgcg cttggcttcg
      781 tttccggcga cgacggctgg ctgttgaacc tgcccgaacc gatttcgttc gagctggaca
      841 aggacacgct caaaccgagc atgcaggaat cgatcgcgac cacggccgcg ggtctgctca
      901 aggcgaacgt gcacaagctg cgcatcgaag gccacaccga cgactccggg ccacgcgact
      961 acaacgtcgc gctgtcgcaa cggcgtgccg agacggtcgc gcaggaattc atcagccatg
     1021 gctttgcacg caccgacgtc gtcgaagtcg gcatgggccc ggatcatccg ttgcaaccga
     1081 acgacacacg caaaaaccgc gccgccaacc gctgcgtcgt catcatcgtt ccagccgagg
     1141 cgctcaatca gtaatgcgga cggcgcgcgc gccggtgcgc t
//
LOCUS       DASRVO010000370         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_114837, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000370 DASRVO010000000
VERSION     DASRVO010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114837"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..495
                     /locus_tag="VFF05_04630"
     CDS             67..495
                     /locus_tag="VFF05_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HZW51123.1"
                     /translation="MPKSLATDIDTPEEERDILQLITLRSQGQFNTTVYSYRDRFRGV
                     EGTRMVLFMNDNDLDRLELKPEQMVSLVTAAGDHVHRQVDGFIVRPYDIPEGCIAGYY
                     PECNPLVPLWHHEERAKVPATKSVPVRVVKTGLGPAEGNL"
     gene            492..>1170
                     /gene="fdhD"
                     /locus_tag="VFF05_04635"
     CDS             492..>1170
                     /gene="fdhD"
                     /locus_tag="VFF05_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZW51124.1"
                     /translation="MTAAASHRVHRRLFRGGIESLGARAVPEETPIAFTYRGCSYAVM
                     MATPADLEDFAIGFSLTEGVAASREEIEEIAVRPQDDGIVLDISLADAAMDRFWNRRR
                     LLAGPSGCGLCGIESIAQALRPVAHVRGDRLLAQSDIVEAVAALPRHQSLNRETRAVH
                     AAALWQSGRGMVALREDVGRHNALDKLVGALAGSSAGLQDNILLLTSRISVELVQKAC
                     AAGATTIA"
BASE COUNT          208 a          383 c          376 g          203 t
ORIGIN      
        1 ttccatcggc cgctcggtgc caagcatcgc gaatggaaga ccaagaccgg caaggccaat
       61 ttcatggtgc cgaagagcct cgcgacggat atcgacacgc cggaagagga acgcgatatc
      121 cttcagctga tcactttgcg cagccaagga cagttcaaca cgaccgtcta ttcctatcgc
      181 gatcgcttcc gcggcgtcga gggcacgcgg atggtgctgt tcatgaacga caatgacctg
      241 gatcggctcg aattgaagcc ggagcagatg gtgagcctcg tgacggccgc cggcgaccat
      301 gtccaccgcc aggtcgatgg cttcatcgtg cggccctacg acattcccga aggctgtatc
      361 gccggctatt accccgaatg caatccgctc gttccgctct ggcatcacga agagcgcgcc
      421 aaggtcccgg cgacgaaatc ggtgccggtg cgcgtcgtca aaaccgggct tgggccggcg
      481 gaaggcaatc tgtgaccgcc gcggcgtcgc atcgcgttca tcgccggctg ttccgcggcg
      541 gaatcgagag cctcggcgcg cgcgccgtcc ctgaggagac gccgatcgcc ttcacctatc
      601 gcggctgcag ctatgccgtg atgatggcga cgccggccga tctcgaggat ttcgccatcg
      661 gcttcagcct gaccgaaggc gttgccgctt cccgcgagga gatcgaggag atcgcagtcc
      721 ggccgcaaga cgacggcatc gtgctcgata ttagcttggc cgatgcggcg atggaccgat
      781 tctggaaccg gcgccgtctc ttggcggggc cgagcggttg cggtctttgc ggcatcgaaa
      841 gcatcgctca ggccttgcgt ccagtcgcgc atgttcgggg cgaccgtctc ctcgcgcaat
      901 cggacatcgt cgaagcggtt gcggcgctcc cccgccatca gtcgctcaat cgcgaaaccc
      961 gtgccgtcca cgcggccgcg ctctggcaat ccgggcgcgg gatggttgcg ctgcgcgagg
     1021 acgtcggacg ccacaacgcg ctcgacaagc tggtcggcgc gctggccggc agcagcgccg
     1081 gccttcagga caacatcctg ttgctcacca gccggatttc ggtcgagctc gtgcagaaag
     1141 cctgtgccgc cggcgcaaca acgatcgcag
//
LOCUS       DASRVO010000371         1206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_37657, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000371 DASRVO010000000
VERSION     DASRVO010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37657"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /gene="murF"
                     /locus_tag="VFF05_04640"
     CDS             complement(<1..777)
                     /gene="murF"
                     /locus_tag="VFF05_04640"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZW51125.1"
                     /translation="MNLRLTDVAMWTKGALHGAGVATSGAAVTGIFTDTRKPVADGLF
                     VALKGENFDAHDFLAQAKEAGAAAALVARPIAVDLPQVVVADTLLALGDLASAVRAQR
                     RARVAGITGSNGKTTVKTLLASILAHCGKTHLNAGNLNNEIGLPLSILAMPEDAQFAV
                     LEMGAGKPGDIAYLAAIARPEIAVVNNIAPAHLERMGSLEGIAQTKGAIYAALPAHGT
                     AVINADDAFADYFAGVSGTRRVLRFGLEHAADVRAEISAMG"
     gene            complement(774..>1206)
                     /locus_tag="VFF05_04645"
     CDS             complement(774..>1206)
                     /locus_tag="VFF05_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZW51126.1"
                     /translation="FVVVDYAHTPDALEQALTSLRAHCAGKLICVFGCGGERDQGKRP
                     QMGAIAERLADEIIVTDDNPRGENGDAIVAQIVAGLAQPRRARIQRDRAAAIALALRA
                     AHAGDVVLVAGKGHEPYQEVDGVRRPFDDADVARRTLEARA"
BASE COUNT          194 a          403 c          408 g          201 t
ORIGIN      
        1 gcccattgcc gaaatttcgg cgcgcacgtc ggcggcgtgt tccagaccga agcgcagcac
       61 gcgccgggtt ccggaaactc cagcaaaata atccgcaaac gcatcatcgg cgttgatcac
      121 ggctgtgccg tgtgcgggca gggccgcgta gatcgcgccc ttggtttgcg cgatgccttc
      181 gagcgaaccc atgcgctcca ggtgcgcggg cgcgatgttg ttgacgacgg cgatttccgg
      241 acgcgcgatg gcggcgagat aggcgatgtc gccgggtttg ccggcaccca tttccagcac
      301 cgcgaattgc gcgtcttccg gcatggccag aatcgacagc ggcaggccga tctcgttgtt
      361 gagattgccg gcattgaggt gcgtcttgcc gcaatgcgcg aggatggatg cgagcagggt
      421 cttgaccgtg gtcttgccgt tggagccggt gatgccggcc acgcgcgcgc gacgttgcgc
      481 gcgcaccgcg ctggcgaggt cgccgagcgc gagcaaggtg tcggcgacaa caacctgcgg
      541 caggtcgacg gcaatgggcc gggcaacgag cgctgcggcc gcaccagcct ctttggcctg
      601 cgcgagaaaa tcgtgggcgt cgaaattctc gcccttgagc gcgacgaaca agccatccgc
      661 aaccggcttg cgcgtgtccg taaaaattcc ggtcacagcc gcgccgctgg tcgcgacgcc
      721 ggcgccgtgc agcgcgccct tcgtccacat ggcaacgtcg gtcaggcgca ggttcatgcg
      781 cgggcctcca gcgtgcgccg cgcgacgtcc gcatcgtcga atgggcgcct gacgccatcg
      841 acttcctggt acggctcgtg gcccttgccc gcgaccagca ccacgtcgcc ggcatgcgcc
      901 gcgcgcaacg caagcgcgat ggcggcagcg cggtcgcgct ggatgcgcgc gcgtcgcggc
      961 tgggcaaggc ccgccacgat ctgcgcaacg atggcgtcgc cattttcgcc gcgcggattg
     1021 tcatcggtga cgatgatttc atcggccagg cgttcggcaa tcgcgcccat ctgcgggcgc
     1081 ttgccctgat cgcgctcgcc gccgcaaccg aacacgcaaa tcaattttcc cgcgcagtgc
     1141 gcgcgcaagc tggtcagcgc ctgctccagt gcatcgggcg tgtgcgcata gtcgacgacc
     1201 acaaac
//
LOCUS       DASRVO010000372         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_271016, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000372 DASRVO010000000
VERSION     DASRVO010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271016"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFF05_04650"
     CDS             <1..836
                     /locus_tag="VFF05_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HZW51127.1"
                     /translation="MSSVRRARPWLGTLVDIRVDGLETACALRAVDAAFAEVAMVHRL
                     MSFHSDDSDLARLRHASVGAAVRVNARTGEVLDLSLRIAAASAGAFDPTVAAALVENG
                     ILPVPHSACVSDIDASWRDIELLGGERVRLRRPLWIDLGGIAKGYAVDRAIEVLRAHG
                     AIDACVNAGGDLRVTGALRERVHVRDAWGGIASALELADGAVATSSCTSAHRDGASRR
                     AAEPDISVSVVAPRCMVADALTKVVLAGGDMAPSVLAAFGAQAWTHRIAHGWRKLDRA
                     A"
     gene            833..1417
                     /locus_tag="VFF05_04655"
     CDS             833..1417
                     /locus_tag="VFF05_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51128.1"
                     /translation="MKRTRRKTLRSRGHFSRRQKFVIYGVLGLLWGSGTLWLVFHYFL
                     QQRGPFGNMPNPAERWLIAIHGASAFAALWLGGWLWHAHVAPWWRNGQRRRSGMFLIA
                     LGGALIVSGYALYYANDDALRRIASTTHWLLGLLFVLPLLVHAMRSGRYRALQRTSAL
                     QEPHQHHLAAAIEDGALARRIRQHHDSLASLDDR"
     gene            complement(1304..1852)
                     /locus_tag="VFF05_04660"
     CDS             complement(1304..1852)
                     /locus_tag="VFF05_04660"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HZW51129.1"
                     /translation="MPWKNGGGWTTQLAAFPESGTNAVAPFDWRVSIAEIESDGAFSA
                     FPGYDRHIALLEGIGMELRFENAETQRLDQRLRFVPFAGEAPVYGKLVSGPVRDFNVM
                     TRRDSCRAEILHRPLVGPMVFFPDATWFVYLAGGTASVKGPGASAIIETGEAIVMLPD
                     ATGERAVLDGGGEVVLVRFLQC"
     gene            complement(1891..2358)
                     /locus_tag="VFF05_04665"
     CDS             complement(1891..2358)
                     /locus_tag="VFF05_04665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HZW51130.1"
                     /translation="MILPRYRVSPSLIPGAGMGLFLDEAVAAGKLITAPDDIHKVYKW
                     QEIESLPDARAVLASTVRWFEDRYTVTPEWPDECYINHSFDPCGLWHLGFVFALADLP
                     PGTEITVDYRHLLAPGQREDFDDAATGKPIVGFDWRESLVASTAQLHALMGAA"
BASE COUNT          431 a          854 c          888 g          475 t
ORIGIN      
        1 ccatgagcag cgtccgccgc gcgcgcccct ggctcggcac cctcgtcgat atccgcgtcg
       61 acggcctgga aacggcgtgc gcgctgcgcg ccgtcgatgc ggctttcgcc gaagtcgcca
      121 tggtgcaccg gctgatgagt ttccacagcg acgacagcga ccttgcgcgc ctgcgccacg
      181 cgagtgtggg cgcggcggtg cgcgtgaacg cacgcaccgg cgaggtgctg gatttgtcgc
      241 tgcgcatcgc cgcagcatcc gccggagcgt tcgaccccac cgtggctgcc gcgctggtcg
      301 agaacggcat cttgccggtc ccacattcgg cctgcgtttc ggatatcgat gcaagttggc
      361 gcgacatcga actgctcggc ggcgaacgcg tgcgcctgcg ccggccgttg tggatcgacc
      421 tcggcggcat cgcgaaaggt tacgcggtgg accgcgccat cgaggtgctg cgtgcgcatg
      481 gcgcgatcga cgcctgcgtg aatgccggcg gcgatctgcg cgtgaccggc gcgcttcgcg
      541 agcgcgtgca cgtgcgcgat gcgtggggcg gaatcgcgtc ggcgctggaa cttgccgacg
      601 gcgcggtggc gacgagcagt tgcacaagcg cccaccggga tggcgcgtcg cgccgggccg
      661 cggaaccgga catttccgtc agcgtggtcg ccccgcgttg catggtcgcc gatgcgttga
      721 ccaaggtcgt gctcgccggt ggagacatgg cgccttccgt gctcgccgct ttcggcgcgc
      781 aggcatggac gcatcggatt gcgcacggtt ggcgcaaact ggatcgcgct gcatgaaacg
      841 cacgcgccgc aagaccctgc gcagccgcgg ccacttttcg cggcggcaga agttcgtcat
      901 ctacggcgtt ctcggcctgc tttggggcag cggcacgcta tggctggtgt tccactattt
      961 cctgcagcaa cgcgggcctt tcggaaacat gccgaatccc gccgaacgct ggctgatcgc
     1021 gattcatggc gcgagcgcgt tcgcggcgct gtggctcggt ggctggctgt ggcacgcgca
     1081 cgtcgcgccg tggtggcgca acggccagcg ccgccgcagc ggcatgttcc tgattgcctt
     1141 gggcggcgcg ctgatcgtca gcggctatgc cctgtattac gccaacgacg acgcgcttcg
     1201 tcgcattgcc tcgaccacgc actggttgct tggcctgctg ttcgtgttgc cgcttttggt
     1261 gcacgccatg cgcagcgggc gttatcgcgc gctgcaacga acttcagcac tgcaggaacc
     1321 tcaccagcac cacctcgccg ccgccatcga ggacggcgcg ctcgcccgtc gcatccggca
     1381 gcatcacgat agcctcgcca gtctcgatga tcgctgacgc gccggggcct ttcacgctcg
     1441 ccgtaccgcc ggcgaggtaa acgaaccagg tcgcatcggg gaaaaacacc atcggcccga
     1501 ccagcggacg atgcaggatt tccgcacggc agctatcgcg ccgcgtcatc acgttgaaat
     1561 cgcgcacggg tccggaaacg agtttgccgt ataccggcgc ctcgcccgcg aatggcacga
     1621 agcgcaaacg ctgatccagg cgctgtgttt cggcgttctc gaagcgcagt tccatgccga
     1681 tgccttcgag cagggcgatg tggcggtcgt agcccggaaa cgcggaaaac gcgccgtcgc
     1741 tctcgatctc ggcgatgctc acgcgccagt cgaatggcgc aaccgcgttc gtgccggatt
     1801 ccggaaacgc ggcaagttgc gtggtccagc cgccgccatt cttccagggc atgcgccggt
     1861 aatcggcacg gcgcagtacg cgcattcgca tcaggcggcg cccatgagcg cgtgcaactg
     1921 cgcggtggaa gcgaccaggc tctcgcgcca gtcgaagccg acgatgggtt tgccggtggc
     1981 ggcatcgtcg aagtcttcgc gctggcccgg cgcgagcaag tgccggtaat ccacggtgat
     2041 ctcggtgccg ggcggcaaat ccgcgagcgc gaacacgaag cccagatgcc acagtccgca
     2101 aggatcgaag gaatgattga tgtagcactc gtccggccat tccggggtga ccgtgtagcg
     2161 atcctcgaac cagcgcacgg tggaggcgag cacggcgcgc gcatcgggca gggattcgat
     2221 ctcctgccat ttgtatactt tgtggatgtc gtcgggtgcg gtgatcagct tgcccgccgc
     2281 gacggcttcg tcgaggaaca ggcccatgcc cgcgccggga atcaaggaag gggaaacgcg
     2341 gtagcggggc aggatcatgg cggcggcttg attttgcggc gacggatgat cgcacaaagg
     2401 atgggcggcg ggtggggaag cccaacgcca tgctcacttg atccagaaaa tgatctcagg
     2461 cagctaccgg ccaatttgag tcattcgctc cgctcgactc gatggcaaac ggctgtcgtt
     2521 tctcaatgcc cagtcgattc ttctgtcgta caagaggaag tcttttgctg cctcaactcg
     2581 cggcgtctcg cccatcgatg ttcagatcgc caaactctac tcgttgttgg cggttgaatc
     2641 gtccgata
//
LOCUS       DASRVO010000373         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_374224, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000373 DASRVO010000000
VERSION     DASRVO010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374224"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFF05_04670"
     CDS             <1..231
                     /locus_tag="VFF05_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010374685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZW51131.1"
                     /translation="RYARLADEVRRILAYAITRGGTTLRDFINPDGAPGYFEQELFVY
                     GRAGAPCKVCATPIRAIVLGQRSTFYCPRCQR"
     gene            complement(233..868)
                     /locus_tag="VFF05_04675"
     CDS             complement(233..868)
                     /locus_tag="VFF05_04675"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HZW51132.1"
                     /translation="MAKLYFYYSAMNAGKTTTLLQSAHNYRERGMRTLILTPQLDDRH
                     GRGVVASRIGLKARGRTFVHNDDLLELVREDIAANGALHCVLVDEAQFLTRAQAWQLS
                     DVVDKLRIPVLAYGLRTDFRGEPFEGSQYLLAWADEMQEIKTICHSGKKATMVVRVDD
                     RGRAVTDGPQVEIGGNERYVSVSRAEFKKIVRGEGRIELHQPGLPLNEKKS"
     gene            1047..>1526
                     /locus_tag="VFF05_04680"
     CDS             1047..>1526
                     /locus_tag="VFF05_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZW51133.1"
                     /translation="MLKLLFAAALFHLPGAAVQATTFTVTANANLSFTPATLAIDVGD
                     TVIFKNSGGGLHNVVANDNSFTNGAPSSANWSFSHTFNSAGTVGYYCSLHGSPGSGMH
                     GAITVNSTAPPPPAIALGGYMSGNWYDASQSGHGFELEFTGQPSGTGNLNTFVAYWYV
                     "
BASE COUNT          248 a          514 c          469 g          295 t
ORIGIN      
        1 cgttacgcgc gcctcgcgga cgaggttcgc cgcatcctcg cttacgccat cacgcgcggt
       61 ggcaccacgc tgcgcgattt catcaatccg gacggcgcac cgggctactt cgaacaggaa
      121 ctgttcgtct acggccgcgc cggcgcaccg tgcaaggttt gcgcgacgcc gatccgtgcg
      181 atcgtcctgg ggcagcgatc gacgttctat tgtccgcgct gccaacgctg actcaggatt
      241 ttttctcgtt caatggaagg ccgggctggt gcagctcgat gcgaccctcg ccgcgcacga
      301 tcttcttgaa ctcggcgcga ctcaccgaca cgtagcgctc gttgccgccg atttccacct
      361 gcggaccgtc ggtgacggcg cggccgcggt cgtccacgcg caccaccatg gtcgccttct
      421 tgccgctgtg gcagatcgtc ttgatctcct gcatctcgtc ggcccaagcg agcaggtact
      481 ggctgccctc gaagggctct ccgcggaaat ccgtgcgcag gccgtaggcg agcacgggga
      541 tgcgcagctt gtcgaccacg tcggacaact gccacgcctg ggcgcgagtg agaaattgcg
      601 cctcgtccac cagcacgcag tgcaacgcgc cgttcgcggc gatgtcctcg cgcaccaact
      661 ccagcagatc gtcgttgtgg acgaaagtcc gtccgcgcgc cttcaggccg atgcgcgagg
      721 cgaccacgcc gcggccgtgg cgatcgtcga gttgcggcgt caggatcagt gtgcgcatgc
      781 cgcgctcgcg gtagttgtgc gcactttgca gcagcgtcgt ggtctttccc gcgttcatcg
      841 ccgaataata gaaatacagt tttgccatgc cgcttgccgt gtccgccgca cgccggcggc
      901 ttcgcgcaca gtgtaaggca ccaaccacgg aagggtggcc gccaccgact ttggtcggta
      961 ccgccgcctc ggcacggatg gcagattgtc ggtgtgcgcg gcgttccgcg cgttgccggt
     1021 agcgtgtcat gttgcggaga actgccatgt tgaaactgct attcgcggcg gccttgttcc
     1081 atcttccagg cgctgcggtg caggcgacca cgttcacggt aactgccaat gcgaacctga
     1141 gcttcacgcc ggcaacgctc gccatcgacg tcggcgacac ggtgatcttc aagaactccg
     1201 gcggcggact ccacaacgtc gtcgcgaacg acaactcgtt caccaacggg gcgccgtctt
     1261 ccgccaactg gagtttttcg catacgttca attccgctgg cacagtgggc tattactgct
     1321 cgctccatgg cagccccggc tccggcatgc acggcgcgat tactgtcaat tcgacggcgc
     1381 ctccgccgcc tgcgatcgcg ctgggcgggt acatgagcgg caactggtac gacgccagcc
     1441 agagcggcca tggcttcgaa ttggaattta cgggccagcc cagcggcacc ggcaacctga
     1501 acaccttcgt cgcttactgg tacgtc
//
LOCUS       DASRVO010000374         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_193569, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000374 DASRVO010000000
VERSION     DASRVO010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193569"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFF05_04685"
     CDS             <1..267
                     /locus_tag="VFF05_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51134.1"
                     /translation="MQKLLAAHANAADMQPSDKVALLNAQEEINGILNHNDNNRLVCE
                     HVAPVGSHRPITTCHTYAEIMQQRERTQHDMERRLSTPQLKSGG"
     gene            305..718
                     /locus_tag="VFF05_04690"
     CDS             305..718
                     /locus_tag="VFF05_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZW51135.1"
                     /translation="MSPRHSSESQWPTAAELEILRVLWDRGPSTVREVHDALYADDGA
                     GYTTALKLMQIMHAKGLVERNDSQRAHVYRAGINKQHTQKRYLTDMARRLFDGSTPQM
                     VLHALGSRPGASREELAEIRALLNKIEAEAEGAKS"
     gene            715..2316
                     /locus_tag="VFF05_04695"
     CDS             715..2316
                     /locus_tag="VFF05_04695"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZW51136.1"
                     /translation="MSLPADWFVAIDVLGVALLHFLWQGAAVGLLYFLLRPLCVRAVT
                     RYGLGLCLLAVLALCPILTLAWLWPEASAAVPTAGSFDSVTAVAAQELPRWQLRPLLP
                     WLVALWFCGVLAIAARSLWQWRNLQILVRGAGRPGAAWELRLDYLRARFGVHRAVRLL
                     SSARVLTPMLVGWIKPVILLPASLFSGFPPQQVELIIAHELGHIRRWDYLANLVQVVI
                     ETVLFYHPVVHWISRDVRNAREECCDDLVLQVASGKALAYARALAGLEELRLDPGFAV
                     PALGAGGGELLARIRRIVGVGAMAQPAPRNAMLPLLLAAAALVFGTWRVQQRDADLAV
                     ALEALPAQALALVSGNPQLIAPPKWAVSLPAPEIAPLAPIALGESAADAAIRIAPAHA
                     SIAGVAFDRIRDLTPDRVRLQVSLPELGEAIAPPNAELAPLQIVPPAYPSRAMVAGIE
                     GSVELSFRIGTDGGVTQVHVVHAQPAGMFEDAAKAALGAWRFPASAAGQVRTQNFAFT
                     LHGKTGVDDKCQSPTGTLICRHPGD"
     gene            2592..>2879
                     /locus_tag="VFF05_04700"
     CDS             2592..>2879
                     /locus_tag="VFF05_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017169438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW51137.1"
                     /translation="MRILVIEDNSDIAANIGDYLEEKGHVVDFAGDGVTGLHLAVVHE
                     FDAIVLDLTLPGMDGLEVARKLRHEAHKQTPILMLTARDALEQKLTGFDSGA"
BASE COUNT          487 a          926 c          927 g          539 t
ORIGIN      
        1 atgcagaaat tgctcgccgc gcatgcgaat gcggcggaca tgcagccgtc ggacaaggtc
       61 gccttgttga acgcgcaaga ggaaatcaac ggcatcctca accacaacga caacaaccgc
      121 ctggtgtgcg aacacgtcgc cccggtcggc tcgcatcgcc cgatcaccac ctgccacacc
      181 tacgccgaga tcatgcagca gcgcgagcgt acccagcacg acatggagcg caggctgagc
      241 acaccacaat tgaagagcgg cggttgagga gtaagattgc attccgcatg acggagcctc
      301 gcgcatgagt cccaggcaca gttccgaatc gcaatggccg acagcggccg agctggaaat
      361 cctgcgcgtg ctctgggatc gcggcccctc caccgtgcgt gaagtgcacg atgcgcttta
      421 cgccgacgac ggcgccggtt acaccaccgc gctcaagctc atgcagatca tgcacgccaa
      481 ggggctggtc gagcgcaacg attcgcagcg cgcgcacgtg tatcgcgccg ggatcaacaa
      541 gcagcacacg caaaagcgtt acctcaccga catggcacgc cgtctgttcg acggctcgac
      601 gccgcagatg gtgctgcacg cgctcggcag ccgtcccggc gcttcccgcg aggaattggc
      661 cgagatccgc gcgctgctca acaagatcga ggctgaggcc gaaggagcca agtcatgagc
      721 ctgccggcag attggttcgt tgcgatcgac gtgctcggcg tggcgctgct gcatttcctc
      781 tggcagggcg ctgcggtcgg cttgctgtat ttcctgctgc gcccattgtg tgtgcgcgcg
      841 gtcacgcgtt atggcctggg actttgcctg ctggccgtgc ttgcgctttg tccgatcctt
      901 actttggctt ggctttggcc ggaagcgagc gcggccgtgc cgacggccgg cagcttcgat
      961 agcgttaccg cggtcgccgc gcaggaactg ccgcgctggc agttgcggcc gctgctgcca
     1021 tggctggtcg cgctgtggtt ttgtggcgtg ctcgcgattg ccgcgcgatc actgtggcaa
     1081 tggcgcaacc tgcagattct cgttcgcggt gccggtcgcc cgggtgcggc atgggagttg
     1141 cgcctggatt acctgcgcgc gcgtttcggt gtgcaccgtg cggtgcgctt gcttagctcg
     1201 gcgcgcgtac tcacgccgat gctggtgggc tggatcaagc cggtcatcct gttgccggcg
     1261 agcctgttca gcgggttccc gccgcagcag gtggaactga tcattgccca cgaattgggc
     1321 catatccggc gctgggatta tctcgccaat ctggtacagg tcgtcatcga aacggtgctg
     1381 ttctatcacc cggtagtgca ctggatttcg cgcgacgtgc gcaacgcgcg cgaggaatgt
     1441 tgcgacgatc ttgtcctgca agtcgcttca ggcaaggcac tggcctacgc acgcgcgctg
     1501 gccgggctcg aggaattgcg cctcgacccg ggattcgccg tacccgcact tggtgcgggc
     1561 ggcggcgaat tgctggcgcg tatccgccgc atcgtcggcg tgggcgcaat ggcgcaaccc
     1621 gcgccgcgca atgcaatgct gccgctgctg ttggctgctg cggcactggt attcgggaca
     1681 tggcgagtgc agcagcgcga tgccgatctg gccgtcgcac tggaagcatt gcccgcgcag
     1741 gcgctcgccc tggtcagcgg caatccgcaa ctgatcgcgc caccaaagtg ggccgtgtcg
     1801 ctgccggcgc cggaaatcgc gccattggcg ccgattgcct tgggtgaatc cgcggctgat
     1861 gccgcgatcc gcatcgcgcc cgcgcatgca agcatcgctg gcgtggcgtt cgaccgtatc
     1921 cgcgacctga cgcccgatcg cgtacgtttg caggtttcct tacccgagct tggcgaggct
     1981 atcgctccgc cgaatgccga gctggcgccc ctgcagatcg tgccgccggc ctatccgtcg
     2041 cgcgccatgg ttgccggtat cgaaggcagt gtggaactgt cgttccgcat cgggacggac
     2101 ggcggcgtaa cccaggtgca tgtcgtgcac gcgcaacccg caggcatgtt cgaggatgcc
     2161 gccaaggcgg cgcttggcgc gtggcgtttc ccggcatccg ccgcgggcca ggtgcgtacc
     2221 cagaacttcg ccttcaccct gcacggcaag actggcgtgg acgacaagtg ccaatccccg
     2281 acagggacgt tgatctgtcg ccaccccggc gattaacgaa agcccgcgcc ggttttaacg
     2341 ccgatttaat tgcgcgatct ctagtatcga ccccactgta ggctgctcga cactcctaag
     2401 gtcaggtcag gtgacaggca gattacggta atcggggcag tagctttagg cccaagcgag
     2461 gtgtgttccc ccaatgacac ctccttgggc ctttttattg cctgcggttt ttgtcgattg
     2521 cgccgtggga actcgggcgg gccggtggcg atcaacgaac gactcccgcc aaagcgagta
     2581 ggattgctcc catgcgtatt ctcgtcatcg aagacaacag cgacatcgcc gccaacatcg
     2641 gcgactacct ggaagaaaag ggccacgtcg tggatttcgc cggcgacggc gtcaccggcc
     2701 tgcacttggc cgtggtccac gaattcgacg cgatcgtgct cgacctgacc ctgcccggca
     2761 tggacggcct ggaagtggcg cgcaagctgc gccacgaggc gcacaagcag acgccgattc
     2821 tcatgctcac cgcgcgcgac gcgctggaac aaaagctcac cggtttcgat tccggcgcc
//
LOCUS       DASRVO010000375         1371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_165864, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000375 DASRVO010000000
VERSION     DASRVO010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165864"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..>1371)
                     /locus_tag="VFF05_04705"
     CDS             complement(94..>1371)
                     /locus_tag="VFF05_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZW51138.1"
                     /translation="RNVERFASAQASREFAQAHALWSRLPKPELLLLAAIFHDIAKGR
                     GGDHSELGETEARTFCERLKLLPADIELVAWLVRRHLLMSVTAQRQDIADPEVVRRFA
                     AEVADWEHLDYLYLLTCADISGTSAKLWNSWKDKLLSDLYVSTRYALRAGLENIPRAG
                     ERADECRREARAILREGGMDAATIERVWADFPDESFLRFTPAQIAWQTRGIAQASADG
                     LHVLVEAKGPRGGTEVFVHATDRDGLFAAITAVFDRLRLSVLEARVVTSRSGMSLDSF
                     VVLDGEGRALRDAAQAARLRNALAKALAQTPYRVEAALHAPSRVQRHFPIAAKIEFGA
                     QPASGHTQLAIVCADRPGLLAAIARVFREQGVRVHDARIATFGERVEDFFLITDEFNQ
                     PLQAQARHDLGTALTTSIDEAAAASLEGSYVIA"
BASE COUNT          217 a          453 c          471 g          230 t
ORIGIN      
        1 gccagcacgt cttcgatcgc cggccgcaac tgcttttcga tgcgcgagcg ggtcagcccg
       61 gcgcggcgtt cccaggcaag gtcgatcgtg tgttcaagcg atgacatagc taccctcgag
      121 tgatgcggcc gctgcctcgt cgatgctggt ggtcagcgcc gtgccgaggt cgtgccgggc
      181 ttgcgcctgc agcggctggt tgaattcgtc ggtgatcagg aagaagtcct cgacgcgttc
      241 gccgaacgtg gcgatgcgcg cgtcgtggac gcgcacgccc tgctcgcgga aaacgcgcgc
      301 gatcgcggcc agcagtccgg gacggtcggc gcacacgatg gcgagttggg tgtggccaga
      361 cgccggttgg gcaccgaact cgatctttgc tgcaatcggg aaatgccgct gcacgcggct
      421 cggcgcgtgc aacgccgctt cgacgcgata cggcgtttgc gccagcgcct tggccagtgc
      481 gttgcgcaag cgcgcagcct gcgcggcatc gcgcagggcg cggccttcgc cgtcgagcac
      541 gacgaagctg tccaggctca tgccgctgcg cgaggtgacc acgcgcgcct cgagcacgga
      601 caggcgcagg cggtcgaaca cggcggtaat cgccgcgaac aggccgtcgc ggtcggtggc
      661 atgcacgaac acctcggtgc cgccgcgcgg tccctttgcc tcgaccagta catgcaggcc
      721 gtcggccgat gcctgcgcga tgccgcgcgt ctgccaggca atttgcgccg gggtgaaacg
      781 cagaaagctc tcgtccggaa agtccgccca gacgcgctcg atggtcgccg cgtccattcc
      841 gccctcgcgc aggatagccc gtgcctcgcg ccggcactcg tccgcgcgct cgcctgcgcg
      901 gggaatattt tccaagcccg cgcgcaacgc atagcgcgtg cttacgtaca ggtcggaaag
      961 cagtttgtcc ttccacgaat tccacagctt cgcgctggtg ccggaaatgt cggcgcaggt
     1021 gagcaggtac aggtagtcca ggtgttccca gtcggcgact tcggcggcaa aacggcgcac
     1081 cacctcagga tcggcgatgt cctggcgctg cgcagtcacg ctcatcagca ggtgccggcg
     1141 caccagccag gcgacgagtt cgatgtccgc gggcaacaat ttcaggcgtt cgcagaatgt
     1201 gcgcgcctcg gtctcgccga gttcggaatg atcgccgccg cgccccttcg cgatgtcgtg
     1261 gaaaatggcg gcgagcagca agagttcagg cttcggcagg cgtgaccaca acgcgtgcgc
     1321 ctgtgcgaat tcgcggctcg cctgcgcgct ggcgaagcgt tcgacgttgc g
//
LOCUS       DASRVO010000376         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_297680, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000376 DASRVO010000000
VERSION     DASRVO010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297680"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..223
                     /locus_tag="VFF05_04710"
     CDS             2..223
                     /locus_tag="VFF05_04710"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZW51139.1"
                     /translation="MVNFEKSLDELEKLVGKMEQGEQSLDDSLKSYERGVALYRNCQS
                     ALEQAELRVKLLSDPEQPDQAQDFEPEAP"
     gene            204..1103
                     /locus_tag="VFF05_04715"
     CDS             204..1103
                     /locus_tag="VFF05_04715"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZW51140.1"
                     /translation="MSRKRLEPPTALIDFSARADQVLARALPPEERAPAPLHRAMRYA
                     VLGGGKRLRPVLVYATGSALGASLDALDASAAAVEIIHAYSLVHDDLPAMDDDALRRG
                     RPTCHVVFGEAMAILAGDALQALAFELLAADKAHAAGTHVEMLRTLAAACGSHGMAGG
                     QALDLAAVGTLLTPDELDRMHAHKTGALIRASVRLGALAAGNTNAATLATLDEYGHAI
                     GLSFQIRDDILDVEGDSATLGKTAGKDAADSKPTYPAILGMNASRARLAKLTDAAIAA
                     LAPLGERAQELMELARYVAERES"
     gene            complement(1104..>2430)
                     /gene="pmbA"
                     /locus_tag="VFF05_04720"
     CDS             complement(1104..>2430)
                     /gene="pmbA"
                     /locus_tag="VFF05_04720"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HZW51141.1"
                     /translation="DSSAQLDRLAELAQDVVRRCRTAGASDVEVGASIDTGLAVNVRL
                     GEVETIEHNRDRAFGVTVYFGQRKGSASTADLAPDSIAKTIEQACAIAKYTEADPFAG
                     LADPARLARAFPDLDLWHPWNLDAQAAIAIGIEIEEAGRALDGIANSEGASVQSGESL
                     GVYATSQDFVGRERGTRHSLSCSLITQDGNGMQRDYWYESVRAAGDFPEVATIGSKAA
                     ERTLARRDARKLDTRQCAVLFAPEVARGLIGHLVSAVSGGALYRKASFLLDHAGKQLF
                     PDFVEITERPHIPRGQGSGNFDSEGVATADSDLIRGGVLQRYVLGSYSARKLGLQSTG
                     NAGGVHNLIVKTGADDFAGMLKKLGTGLLVTEVMGQGVSIVTGDYSRGASGFWVENGA
                     IAYPVEEITIASNLREMFAGIAAIGADVDYRSHILTGSILIERMMVAGS"
BASE COUNT          438 a          835 c          754 g          403 t
ORIGIN      
        1 catcgtcaat ttcgaaaaat cgctcgacga actcgagaaa ttggtcggca agatggaaca
       61 aggcgaacaa agcctggacg attcgctcaa atcctacgaa cgcggcgtcg ccctgtaccg
      121 caattgccag agcgcgctcg aacaagccga actgcgcgtg aagctgctgt ccgatccgga
      181 acagcccgac caagcccaag actttgagcc ggaagcgcct tgagccgccc accgcgctga
      241 tcgacttcag cgcgcgcgcg gaccaggtgc tggcgcgcgc gttgccgcct gaagaacggg
      301 cgcccgcgcc actgcaccgt gccatgcgct acgctgtgct tggcggtggc aagcgcctgc
      361 gcccggtact ggtttacgcg accggcagcg cgctcggtgc gtcgctcgac gcactcgatg
      421 catccgccgc cgcagtggaa atcatccacg cctattcact ggtccacgac gatctgccgg
      481 cgatggacga tgacgccctg cgccgtggcc gacccacttg ccatgtcgtc ttcggcgaag
      541 cgatggccat cctcgccggc gacgcgctgc aggcgctggc cttcgaattg ctggccgcgg
      601 acaaggcgca cgccgccggc acacatgtgg aaatgttgcg cacgctcgcc gccgcctgcg
      661 gttcacatgg tatggccggc gggcaggcgc tcgacctcgc cgccgtcggc acgctgttga
      721 ccccggacga gctcgatcgc atgcacgcgc acaagaccgg cgcgttgatt cgcgccagcg
      781 tacgccttgg tgcgctcgcc gccggcaaca ccaacgccgc gacgctcgcc acgctggacg
      841 aatatggcca cgccatcggc ctctccttcc agatccgcga cgacatcctc gatgtggaag
      901 gcgacagcgc caccctcggc aagaccgccg gcaaggatgc cgccgattcc aagcccacct
      961 atcccgcgat cctcggcatg aatgcctcgc gcgcgcgcct ggcgaaactg acggacgcag
     1021 caatcgcggc gcttgcgcca ctcggcgaac gcgcgcagga gctcatggaa ttggcgcgct
     1081 acgtggccga gcgcgagagc tgatcaactt cccgccacca tcatccgctc gatcaggatc
     1141 gagccggtga ggatgtgcga gcggtagtcg acgtcggcgc cgatcgcggc gatgccggcg
     1201 aacatctcgc gcaggttcga ggcgatggtg atttcctcga ccggataggc aatcgctccg
     1261 ttctcgaccc aaaaacccga cgcgccgcgc gaataatcgc cggtgacgat ggaaacgccc
     1321 tgccccatga cctcggtgac cagcagaccg gtaccgagtt tcttcagcat gccggcgaaa
     1381 tcgtcggcgc ccgtcttcac aatcaggttg tgcacgccgc cagcattgcc ggtgctttgc
     1441 aggccaagct tgcgcgccga atagctgccg agcacgtagc gctgcagcac acccccgcga
     1501 atcagatcgc tgtcggcagt ggcgacgcct tcgctgtcga aattgcccga gccttgtccg
     1561 cgcggaatgt gcgggcgttc cgtgatttcg acgaaatccg gaaacagttg cttgcctgcg
     1621 tgatcgagca ggaaactcgc cttgcgatac aacgcaccgc cgctcacggc ggaaaccaga
     1681 tgcccgatca ggccacgcgc gacttccggc gcgaacagca ccgcacactg gcgtgtgtcg
     1741 agcttgcgtg cgtcgcgccg cgcgagcgtg cgttcggcgg ccttgctccc tatcgtggcg
     1801 acttcgggaa aatccccggc ggcacgcacg gattcgtacc agtaatcgcg ctgcatgccg
     1861 ttgccatcct gtgtgatcaa cgagcacgac aacgaatgcc gcgtgccgcg ttcacggccg
     1921 acgaaatcct gcgatgtggc gtagacgcca agtgattcgc cggattgcac ggaagcgcct
     1981 tccgagttgg caattccgtc gagtgcacgg cccgcctcct cgatttcgat gccgattgca
     2041 attgccgcct gcgcatccag attccacgga tgccacaaat ccaggtccgg aaacgcgcgt
     2101 gccagccgcg ccggatcggc gagtcccgcg aacgggtccg cctcggtgta cttcgcgatc
     2161 gcgcaggctt gctcaatggt cttggcgatg gaatcgggtg ccaggtccgc cgtgctcgcc
     2221 gaacccttgc gctggccgaa atacacggtc accccgaagg cacggtcgcg gttgtgttcg
     2281 atggtttcga cctcgcccag gcgcacgttc accgcaaggc cggtgtcgat gctggctccg
     2341 acttcgacgt cgctcgcccc ggcggtacgg cagcggcgca cgacgtcctg cgcgagttcg
     2401 gcgagacgat cgagctgggc ggacgaatcg
//
LOCUS       DASRVO010000377         1079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_140633, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000377 DASRVO010000000
VERSION     DASRVO010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140633"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFF05_04725"
     CDS             complement(<1..754)
                     /locus_tag="VFF05_04725"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZW51142.1"
                     /translation="MPATLPEPAAATAQHSASLAESIRRDIAAHCDRETGGGWIPFSR
                     FMDLALYAPGLGYYAAGAHKFGAAGDFITAPELSPLFGRTLARQVAGITADSAPHILE
                     LGAGSGKLAADMLAELERLGCLPDSYAILEVSADLRARQQDLVRERLPHLLDRVRWLD
                     ALPERFSGAIVANEVLDALPVHLLHWRDSAITERGVAAGEHGFIWQERAVGDPALLHA
                     ARQIAVPDDYASEICLAARGLISSLAQSLEQGA"
     gene            813..>1079
                     /locus_tag="VFF05_04730"
     CDS             813..>1079
                     /locus_tag="VFF05_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZW51143.1"
                     /translation="MSLMEQGMQGKVVLVTGGAKRVGAAICRRLHAAGAQIAIHYRSS
                     RQEAQAIQDELNALRPDSAAIFQADLLDLERLPKMFQDVILKFRQ"
BASE COUNT          206 a          346 c          359 g          168 t
ORIGIN      
        1 tggcaccctg ctccaggctt tgcgcgaggc tgctgatcag gccgcgcgcg gcgaggcaga
       61 tttcgctggc gtaatcatcc ggcacggcga tctgccgcgc cgcatgcagc aaagccggat
      121 cgccgaccgc gcgctcctgc cagatgaagc cgtgttcacc ggcagcaacg ccgcgctcgg
      181 tgatcgcgct atcgcgccag tgcagcagat gcaccggcaa cgcatcgagc acttcgttcg
      241 cgacaatcgc gccactgaat cgttccggca atgcatccag ccagcgcacc cggtccagca
      301 gatgcggcag gcgttcgcgg accaggtctt gctggcgcgc acgcaggtcg gcgctgacct
      361 cgaggatcgc ataactgtcc ggcaggcagc caagccgctc cagttcggcg agcatatccg
      421 ctgccagttt gccgctgcct gcgcccagct ccaggatatg cggcgcgctg tcggcggtga
      481 tcccggcaac ctgccgcgcc agcgtgcgcc cgaacagcgg cgagagttcc ggtgcggtga
      541 tgaaatcgcc cgccgcgccg aacttgtgcg cgccggccgc gtaatagccg agtcccggcg
      601 catagagcgc cagatccatg aaacgcgaga acggaatcca cccgcccccg gtttcgcgat
      661 cgcaatgcgc agcgatgtcg cgccggatcg attcggccag ggatgcgctg tgttgcgcgg
      721 ttgcagcggc aggctcgggt aaagtagcgg gcatggtcag gtataattcc cgaaacagcg
      781 ggatcgaacg gtatggccgg gttgaaagga gcatgagtct aatggaacag gggatgcagg
      841 gcaaagtggt attggtgacg ggaggcgcca aacgcgtcgg cgcggcgatc tgccgccgcc
      901 tgcacgcggc cggcgcgcag atcgccatac actaccgcag ctccagacag gaggcgcagg
      961 cgatccagga cgagctgaat gcgctgcgcc cggacagcgc ggcaatattc caggccgatc
     1021 tgctcgatct ggaaagattg ccgaaaatgt tccaggacgt cattctgaaa ttccggcag
//
LOCUS       DASRVO010000378         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_63076, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000378 DASRVO010000000
VERSION     DASRVO010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63076"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFF05_04735"
     CDS             complement(<1..281)
                     /locus_tag="VFF05_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010485832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZW51144.1"
                     /translation="MTRRQTTRAPNLFPESDALKPLAERMRPRTLDEIVGQTRLLGPG
                     TALRRSIEAGHVHSMVLWGPPGCGKTTLALLLAKYAKAEFRAISAVLSG"
     gene            complement(300..521)
                     /locus_tag="VFF05_04740"
     CDS             complement(300..521)
                     /locus_tag="VFF05_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZW51145.1"
                     /translation="MSRENRSARLTILIDPGKKAVFEELCRLEDLTPSQVVRRLIRGH
                     IELKLGRPWKPGEGPTALKPPAHAKRRKA"
     gene            555..>1846
                     /locus_tag="VFF05_04745"
     CDS             555..>1846
                     /locus_tag="VFF05_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZW51146.1"
                     /translation="MIILSDAVKRTAAGGAVERPVERFCMPLPSALHSIFTLVMAWVA
                     LGAAGLLFPRNLTWVSRVLFPCGAVVAAAIAVCAYAALALPPQTATLPIGLPELPMHL
                     RLDALSAFFLLLIGTAVAGVSIYSAGYFRSGEGAVPGLMCLQYHVFLASMAMVVLADD
                     AFAFMVAWETMAVSSYFLVTTQHRVAEIRGAGFLYLLLAHIGAIGLLLCFGVLQGGSW
                     QFTFDAMRAATLTPGWASVAFALALFGFGAKAGLVPLHVWLPEAHPAAPSPVSALMSG
                     VMLKTAVYGCIRVAFDLIHAPQWSWGAAVLALGAVTGLFGAIFAAVQTDMKRLLAYSS
                     IENIGLIFSGLGLALLFRAFGSNALAALALAGTLYHCLNHAQFKSLLFLATGSVLHAT
                     GQRNLGRLGGLIHRMPWVAGFALLGTLAMAGLPPLNGFV"
BASE COUNT          250 a          616 c          594 g          386 t
ORIGIN      
        1 atgccggaca gcaccgccga gatcgcgcgg aattcggcct tggcgtactt tgccagcagc
       61 agcgcgagcg tggtcttgcc gcagcccggc ggcccccaca gcaccatcga atgtacgtgc
      121 cccgcttcga tcgaacgacg caaggcagtg ccgggaccaa gcaggcgcgt ttgcccaacg
      181 atctcgtcga gcgtgcgcgg ccgcatgcgt tcggcaagtg gtttcagcgc gtcggattcg
      241 gggaacaggt tcggagcgcg ggtcgtttgg cggcgggtca tccgcggatg atacggaatt
      301 cacgccttcc tgcgtttcgc atgcgccggc ggtttcagcg cagtcgggcc ctcgcccggc
      361 ttccacggcc ggcccaactt cagctcgatg tggccgcgaa tcaggcggcg cacgacctgc
      421 gacggcgtca gatcctcgag ccggcacagt tcttcgaaca ccgccttctt gccgggatcg
      481 atcaggatgg tcagccgggc gctgcggttc tcgcgtgaca tgcgcaaatc ttatttacat
      541 gcgatacgca cagcatgata atcctgtccg acgctgtcaa gcgcaccgcc gcaggcggcg
      601 cggtggaacg tcccgtcgaa aggttctgca tgccgctgcc gagcgcatta cattcgatct
      661 tcacgctggt catggcctgg gtcgcgttgg gtgcggcggg cctgctgttt ccgcgcaatc
      721 tcacctgggt gagccgcgtg ctgtttccat gcggggcagt ggtcgcggcg gccatcgcgg
      781 tatgcgcgta tgccgcgctc gccttgcccc cgcaaaccgc cacgttgccg attggcctgc
      841 ccgagttgcc gatgcatctg cgcctggacg cactgtccgc gttcttcctg ctgctgatcg
      901 ggaccgccgt cgccggcgtg tctatctatt ccgccgggta tttccgttcc ggcgaaggtg
      961 cggttccggg cctgatgtgc ctgcaatacc acgttttcct cgccagcatg gcaatggtcg
     1021 tgctggccga cgatgccttc gcgttcatgg tcgcgtggga aaccatggct gtgtcgtcgt
     1081 atttcctcgt gaccacccag caccgtgtcg ccgagatccg cggcgccggt tttctctatc
     1141 tgctgctcgc gcacatcggc gcgatcgggt tgctgctgtg cttcggcgta ttgcagggcg
     1201 gctcgtggca attcaccttc gatgcgatgc gcgcggcaac gttgacgccg ggatgggcca
     1261 gcgttgcttt cgcactcgcg ctgttcggct tcggcgccaa ggccggtctc gtgcccctgc
     1321 atgtgtggct gccggaagcg catccggcag caccatcgcc cgtctccgcg ctgatgagcg
     1381 gcgtcatgct caagaccgcg gtgtacggtt gcatccgcgt cgccttcgac ctgattcacg
     1441 cgccgcaatg gagctggggc gcggcggtac tcgcgctcgg cgcggtcacc ggactgttcg
     1501 gcgcgatctt cgccgccgtg caaaccgaca tgaagcgcct gctcgcgtat tcgtcgatcg
     1561 agaacatcgg cctgattttc tccgggctcg gactggcgct gctgtttcgt gccttcggca
     1621 gcaatgcact cgccgcgctg gcgctggcgg gcaccttgta ccactgcctg aaccacgccc
     1681 agttcaagag cctgctgttc cttgccaccg gctcggtgtt gcatgcgacc gggcagcgca
     1741 atctcggccg cctgggcggt ctgatacacc gcatgccctg ggttgccggc ttcgccctgc
     1801 tcggcacgct cgccatggcg ggattgccgc cgctcaacgg tttcgt
//
LOCUS       DASRVO010000379         1269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_348279, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000379 DASRVO010000000
VERSION     DASRVO010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348279"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VFF05_04750"
     CDS             complement(<1..622)
                     /locus_tag="VFF05_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51147.1"
                     /translation="MDQGFRLFRSKAFGEELPLEAATFVEGKLRGGFDGVDGRKRREQ
                     ATLLLARRFASGRENRRVIFRAAKLAGAIARFRRRLARDFARKGDCTGQQVALDQSID
                     DPALQTLRGGNGIALRAHLHRLGHASQPRQALRAVRAGNQAQLHFRLAELRVLRGDAV
                     MPRHSQFEPAAQRVAVNGHHQRLRRILDAIEQIGEAGRSRRFRGTHL"
     gene            707..>1269
                     /locus_tag="VFF05_04755"
     CDS             707..>1269
                     /locus_tag="VFF05_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZW51148.1"
                     /translation="MGAGTMGGGIAMVLANAGIPVLLKETDQTALDRGLAGIRRNYAS
                     SVARGRFTQPFVDERLNLIRPTLTYDGFEQADIVIEAVFESMALKKQVFAELDRIANP
                     GAILATNTSTLNVDEIASATSRAECVIGTHFFSPANVMRLLEIVRGKMTSKPVIATAM
                     QLAKRVGKIGVLVGNCRGFVGNRMFHQ"
BASE COUNT          236 a          417 c          407 g          209 t
ORIGIN      
        1 caagatgcgt tccgcggaag cgccggctgc ggccggcttc cccgatctgc tcaatcgcat
       61 cgagaattcg ccgaagccgc tggtgatggc cattcacggc aacgcgctgg gcggcgggct
      121 cgaactggct atggcggggc attaccgcgt cgccgcgaag gacgcgcagc tcggccagcc
      181 ggaagtgaag ctgggcctga ttcccggcgc gcacggcacg cagcgcctgc cgcggttggc
      241 tggcgtggcc caggcggtga agatgtgcgc ggagggcaat cccatttccg ccgcggaggg
      301 tttgcagcgc gggatcgtcg atcgactgat cgagggcgac ttgctggccg gtgcagtcgc
      361 ctttgcgcgc gaagtcgcgg gcaagccggc gccgaaaacg cgcgatcgcg ccggcaagct
      421 tggcagcgcg gaagataacg cgccgatttt cgcggccgct cgcgaagcgg cgcgcaagaa
      481 gcagcgtggc ctgctcgcgc cgcttgcggc catcgacgcc atcgaagccg ccacgaagct
      541 tcccttcgac gaaggttgcc gcttcgagcg ggagctcttc tccgaatgcc ttgcttcgga
      601 acagtcgaaa gccctgatcc acgtcttctt cagcgaacgc gaagcggcga aaattccgga
      661 tgtccccaaa gagacgccga cgattcccgt acatcgcgct gccgtggtgg gcgcgggcac
      721 gatgggcggc ggcatcgcca tggtgctggc caatgcgggc attcccgtgc tcttgaaaga
      781 aaccgatcag acggcgctcg accgcggcct cgccggcatt cgcaggaact atgcgagttc
      841 cgtcgcgcga gggcgcttca cccagccatt cgtggacgag cggctcaacc tgatccgccc
      901 aactctcacc tacgacggct tcgagcaagc cgacatcgtg atcgaagcgg tgtttgagag
      961 catggcgctc aagaagcagg tcttcgccga actcgaccgc atcgcaaacc ccggcgcaat
     1021 cctcgccacc aacacctcca cgctgaacgt cgacgagatt gcctcagcca cctcgcgggc
     1081 cgaatgcgtc atcggcactc actttttcag cccggccaac gtgatgcgcc tgctcgaaat
     1141 cgttcgcggc aagatgacca gcaaaccggt gattgccaca gccatgcagc tcgccaagag
     1201 ggtcggaaaa atcggcgtgc tggtcggcaa ttgccgcggc ttcgtcggca accgcatgtt
     1261 tcatcagta
//
LOCUS       DASRVO010000380         1450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_193700, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000380 DASRVO010000000
VERSION     DASRVO010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193700"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..>1450
                     /locus_tag="VFF05_04760"
     CDS             182..>1450
                     /locus_tag="VFF05_04760"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZW51149.1"
                     /translation="MGDALARVLEAKGVEALRIDAKIELNALAERLKNWMATGSVHGV
                     YWLPALDIEPRISEMDFAAWHEAARVRVKSLYLTMRTLYEQIAAPGTFLVSATRLGGQ
                     HGYDEAGAVAPLGGAIAGFTKTYKRERADACVKVVDFEPGSKPAEIAGLLVEETLRDP
                     GAVEVGYKSGLRWTIGLQEQPAADGRLGMTLDEKTVFVVTGAAGSIVSAIVADLAAAS
                     GGTFYLLDLVPEPDPNNTDLERFVEDKDGLKRDLFARIQARGERATPALVEKELAALE
                     RAQAARAAMEAVRAAGGTARYFSVDMREGEAVARVIDQVRKNSGRIDVLLHAAGVDRS
                     RALPQKDPGEFDLVFDVKSDGFFNLLHAIGDMPLGASVAFSSIAGRFGNPGQTDYSAA
                     NDLLCKITSNLRSTRPETRALAIDWTAWGGI"
BASE COUNT          267 a          431 c          517 g          235 t
ORIGIN      
        1 atctgccgct gcgacgcaag tcacgaagcc gcgcccgcct ctggccagtc tggacgcggc
       61 gaacagcatt gcgcgccgcg tcccgctgcc gatcctgcgg ccgccgctgg ccatgtgcaa
      121 gccgacagga gtcgcgctcg atggcgggcg ccgtgtggtg gtgatgagcg atagcggcgg
      181 agtgggcgac gctctcgcac gggttcttga agcgaaggga gtcgaagcgc tgcgcatcga
      241 cgcgaagatc gaattgaatg cgctggcgga acgcttgaag aactggatgg ccaccggatc
      301 agtgcacggg gtctattggc ttccggcatt ggacatcgag ccgcgcattt ccgaaatgga
      361 tttcgccgcg tggcatgaag ccgcgcgcgt gcgcgtgaag tcgctgtacc tcacaatgcg
      421 cacgttgtac gagcaaattg cggcgccggg gactttcctg gtatccgcca cgcgccttgg
      481 cgggcagcac gggtacgacg aggcgggagc cgtggcgcct cttggcgggg ccatcgcggg
      541 tttcacgaag acgtacaagc gcgagcgcgc ggacgcctgc gtgaaggtcg tggatttcga
      601 accagggagc aagccggccg aaattgccgg actgctggtg gaagaaacgc ttcgcgaccc
      661 cggagcagtc gaggtcggct acaagtcagg actgcgctgg acgattggct tgcaggaaca
      721 gcccgcagcc gatggccggc tgggcatgac gctggacgag aaaacagtct ttgtcgtgac
      781 cggagcggcg ggcagcatcg tttccgcgat cgtcgcggac ctcgcagcgg catcgggtgg
      841 cacgttctac ctgctcgatc tggtgcccga gcccgatccg aacaacaccg atctggaacg
      901 cttcgtggag gacaaagacg gcctgaagcg cgatctgttc gcgcgcatac aagcgcgcgg
      961 cgaacgtgcg acgccagcgc tggtggagaa agaactggcg gcgctcgagc gcgcgcaggc
     1021 cgcacgcgcc gcgatggagg ctgttcgagc cgccggcgga acggcgcgct atttcagcgt
     1081 cgacatgcgg gaaggcgaag cggtggcgcg cgtcatcgat caggtcagaa agaacagcgg
     1141 gcgcatcgac gttttgctgc atgcagccgg cgtggaccgc agccgcgcat tgccgcagaa
     1201 ggacccgggc gaattcgacc tggtgttcga cgtcaagagc gacggcttct tcaatctcct
     1261 gcatgctatc ggcgacatgc cgctgggcgc cagcgtggcg ttcagctcga ttgccgggcg
     1321 ttttggcaat ccaggccaga ccgattacag cgcggcgaat gacctgttgt gcaagatcac
     1381 ctcgaacttg cgcagcacgc ggccggagac gcgggcgctg gccatcgact ggactgcgtg
     1441 gggcggaatc
//
LOCUS       DASRVO010000381         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_244842, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000381 DASRVO010000000
VERSION     DASRVO010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244842"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFF05_04765"
     CDS             <1..338
                     /locus_tag="VFF05_04765"
                     /inference="COORDINATES: protein motif:HMM:NF019404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HZW51150.1"
                     /translation="ADSSRFREAKGHENPPAPFATTVFAAPGLKLGAFGVDATLLVAQ
                     HAPQAYPVSDSGLPPNLAASFSAANGALYAGGDPAHPQVGMLRVSYRIVPLGAATLEG
                     VQRGGKIVP"
     gene            335..1132
                     /locus_tag="VFF05_04770"
     CDS             335..1132
                     /locus_tag="VFF05_04770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZW51151.1"
                     /translation="MNIERSHERKDIEYHAAIAAEYDRVVVEPRRHSIDALFRPLHKL
                     LPPHKGAMLDLGCGTGHMLCRYAAGFDRVVAVDHSLEMLAGAQSNARNAGLAHVEFDR
                     GDAFEFLRSRRGEKFDLITCVGFLHHLEQRRIAEIFALIESLLAADGLFVFAEPVETA
                     DREPWPITWWNASFRSRPHEYSHAAEDPDEAPLQISRLRQDAASAGLRIVGEGRGWEI
                     FPRHEPPSRLDALAIPLLHRLFGAAGPVYWAACTRAANQEAPNNSAA"
     gene            complement(1102..1644)
                     /locus_tag="VFF05_04775"
     CDS             complement(1102..1644)
                     /locus_tag="VFF05_04775"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZW51152.1"
                     /translation="MTQTQTLFNGQWLRLRKRGRWEFAERTNPGGGVIIVALTPQDHI
                     LFVEQWREAIGAKTIEMPAGLVGDRDGAPGENAVGAARRELLEETGYRAGRVEFLLAG
                     PSSAGMSNEVIAFVRAYDLVREHAGGGDHTENIIVHEVPRHEAPRWLLDKARAGYSID
                     PKLFAGLYFLDHAAELFGAS"
     gene            1737..>2621
                     /locus_tag="VFF05_04780"
     CDS             1737..>2621
                     /locus_tag="VFF05_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51153.1"
                     /translation="MPIQSLRIQSWILAAAVAVMPLATAAQQAATADTAAKAQAELVK
                     RAEALTQSQIAQSHDMAALRRLGQIYAAQNDMPRFIWVLKRVGEMSPNSGELKLQLAL
                     AYAKIDDKPHAYDTLIRMQTQGFGYDISKDKRFEPIHGTKVWDYIVANLNVNAKQFGE
                     GKTAFSLPKGDYLYDALAWDAKRGDVLVGSVRDGSIRRVDAHGKVSDFIKPDADNGLW
                     SVDALAVDAARGKLYAGSSSTAIFQGFSGDNAGEAGLFEFDLGTGKLLKKYAFDAGAG
                     THRLTALAVASDGKVYAAD"
BASE COUNT          452 a          849 c          832 g          488 t
ORIGIN      
        1 cggccgattc gagcaggttt cgcgaggcca agggccatga gaatccaccg gcgccattcg
       61 ccacgactgt gttcgccgca ccggggctca agctcggcgc gttcggcgtc gatgccacat
      121 tgctggtcgc ccagcatgcg ccgcaagcct acccggtcag cgattccgga ttgccgccaa
      181 acttggcggc cagcttcagt gcagcgaacg gcgcgcttta cgccggcggc gatccggcgc
      241 atccgcaggt cgggatgctg cgcgtgagct atcgcatcgt gccgcttggc gcggccacgc
      301 tcgagggcgt gcagcgcggt gggaaaatcg tgccgtgaac atcgaacgtt cgcacgaacg
      361 caaggacatc gaatatcacg ccgccatcgc ggctgagtac gaccgggtcg tcgtcgagcc
      421 gcgccggcat agcatcgatg cgctgtttcg tccgctacac aagttgcttc cgccgcacaa
      481 aggcgcaatg ctggatctgg gctgcggcac cggacacatg ttgtgccgtt acgccgccgg
      541 tttcgatcgt gtcgtcgctg tcgaccacag cttggaaatg cttgcggggg cgcagtcgaa
      601 cgcgcgtaac gcgggcttgg cgcatgtcga attcgatcgc ggcgacgctt tcgaattcct
      661 gcgttcccgg cgcggcgaaa aattcgacct gatcacttgt gttggatttc tgcatcatct
      721 ggagcagcgg cggatcgccg aaattttcgc cctgatcgaa tcgctgttag ctgcggatgg
      781 attgttcgtg ttcgccgagc cggtggaaac cgccgaccgc gagccgtggc cgatcacgtg
      841 gtggaacgca tcattccgct cgcgtccgca cgagtattcg catgccgcgg aggatcccga
      901 cgaagcgcca ctgcagattt cccggcttcg ccaggatgcg gcgagtgccg ggttgaggat
      961 cgttggagaa gggcgcggct gggagatttt tccgcgacac gagccgccga gccgactcga
     1021 cgctcttgct atcccgcttc tgcatcggtt gttcggtgct gctggtccgg tctattgggc
     1081 ggcgtgcacg cgcgccgcga atcaggaggc gccgaacaac tcggcggcgt gatccaggaa
     1141 atacagtcct gcgaacagct ttggatcgat cgagtagccc gcgcgcgcct tgtccagcag
     1201 ccagcgcggc gcttcgtggc gcggcacctc gtgcacaatg atgttttcgg tatggtcgcc
     1261 gccgcccgcg tgttcgcgca cgagatcgta agcgcgcacg aacgcgatga cctcgttgct
     1321 catgcccgcg gacgacggcc cggcgagcag gaattccacg cgtccggcgc ggtagccggt
     1381 ttcctcgagt agttcgcggc gtgcggcgcc gaccgcgttt tcgccgggcg cgccgtcgcg
     1441 gtcgccgacc aggcccgccg gcatctcgat ggttttggcg ccgatcgcct cgcgccattg
     1501 ttcgacgaac aggatgtgat cttgcggcgt caatgccacg atgatcacgc cgccgcccgg
     1561 attcgtgcgt tcggcgaatt cccagcgccc gcgcttgcgc aggcgcagcc attggccgtt
     1621 gaaaagcgtc tgcgtttgcg tcattctggg cagctttttc cgattgtctt gcgcgacgca
     1681 cgaacttcgc cgcggcgccg cggtcatacc cgcctcccat cattacggat tcacccatgc
     1741 caattcaatc gttgcgcatt caatcgtgga ttctggctgc cgcggtcgcg gtcatgccgc
     1801 tcgcgaccgc cgcgcagcag gccgccaccg ccgacaccgc tgccaaggcg caagccgagc
     1861 tggtcaaacg cgccgaagcg ctgacccaaa gccagatcgc gcaatcgcac gacatggcgg
     1921 ccttgagacg tctcggccag atctatgccg cgcagaacga catgccgcgc ttcatctggg
     1981 tgctcaagcg cgtgggcgaa atgtcgccaa attccggtga gctgaagctg caactcgcgc
     2041 tcgcctacgc gaagatcgac gacaagccgc atgcctacga caccctgatc cgcatgcaga
     2101 cgcagggttt cggctacgat atatcaaagg ataaacgctt cgagccgatc catggcacca
     2161 aggtgtggga ttacatcgtc gccaatctca acgtcaatgc caagcagttt ggcgaaggca
     2221 agaccgcatt ctcgctgccc aagggcgatt acctgtacga cgcgctggcc tgggacgcca
     2281 agcgcggcga cgtgctcgtc ggcagcgtgc gcgacggctc gatccggcgc gtggatgcac
     2341 atggcaaggt ttccgatttc atcaagcccg atgcggataa cggtctgtgg agcgtggacg
     2401 cactcgccgt ggatgcggcg cgcggcaaac tctacgcggg cagttcgtcc accgcgatct
     2461 tccagggctt cagcggcgac aatgccggcg aggccggcct cttcgagttc gatctcggca
     2521 ctggcaagct cctaaaaaaa tacgcgttcg acgccggcgc cggcacgcat cgcctgactg
     2581 cgctggccgt cgcttccgac ggcaaggtct acgccgccga c
//
LOCUS       DASRVO010000382         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_11268, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000382 DASRVO010000000
VERSION     DASRVO010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11268"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFF05_04785"
     CDS             complement(<1..415)
                     /locus_tag="VFF05_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HZW51154.1"
                     /translation="MQKLNPADLYTLEAYARERAAFRARVLAHKQARRVQLGAHLTLL
                     FEDRLTIQYQVQEMLRIERIFEEAGIRDELDAYNPLIPDGANLKATLLIEYPDPEVRA
                     RKLVELRDIEHRIALRIGGLAPIAAIADEDMERSNA"
     gene            complement(417..1244)
                     /locus_tag="VFF05_04790"
     CDS             complement(417..1244)
                     /locus_tag="VFF05_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HZW51155.1"
                     /translation="MHPDARVPQYAARTARKRLKNLDGTGDAQPAGRTRGKVAIFATC
                     YCNYSQPEPVEDLAAVLRHNGVAVKLVEREVCCGMPKLELGDLASVEKYKTQNIPVLA
                     AAIRDGWDISATIPSCVLMFKQELPLMFPDDEDVQLVKRHIFDPFEYLWQRHQAGLLK
                     TQFPNPLGKVAYHAPCHQRVQNIGPKTRDVLALVPGTEIQTIERCSGHDGTYGVKAKT
                     YAFSRKIAKPVETRVEQIAPAHFASDCPMAASHIAHGLGDQPPAESPIGLLRRAYGI"
     gene            complement(1187..1759)
                     /locus_tag="VFF05_04795"
     CDS             complement(1187..1759)
                     /locus_tag="VFF05_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51156.1"
                     /translation="MADERSTSREGSLDAPTRHPLDWKNPQFYDQAALEAEMERVFHI
                     CHGCRRCVSLCQAFPTLFDLVDNSATMEVDGVAKSDYAKVVDQCYLCDLCYQTKCPYV
                     PPHPWNVDFPHVMLRAKATAHRRGETKTSAKILSSTTAVGKLATIPVVVEAVNAVNSS
                     GLGRKLLEKALACIPTRACRNTPRARRASA"
     gene            complement(1819..2238)
                     /locus_tag="VFF05_04800"
     CDS             complement(1819..2238)
                     /locus_tag="VFF05_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HZW51157.1"
                     /translation="MATLKGSKTEQSLKDAFGGESMANRRYLYFAQKADVEGYNDVSA
                     VFRSTAEGETGHAHGHLEYLEQCGDPATGLPFGDTKANLKSAIAGETHEYTDMYPGMA
                     KTAREEGFGEVADWFETLAKAERSHANRFTKALNDLA"
BASE COUNT          375 a          751 c          828 g          503 t
ORIGIN      
        1 cggcgttgct gcgttccatg tcctcgtcgg cgatggcggc gatcggcgcg agcccgccga
       61 tgcgcagcgc gatgcgatgt tcgatgtcgc gcagttcgac cagcttgcgc gcgcgcactt
      121 ccgggtcggg gtattcgatg agcaaggtgg ccttgaggtt cgcgccatcc ggaatcagcg
      181 gattgtaggc gtcgagttcg tcgcggatgc cggcttcctc gaagatgcgt tcgatgcgca
      241 gcatttcctg cacctggtac tggatcgtca ggcgatcctc gaacagcagg gtcaggtgcg
      301 cgcccagttg cacgcgtcgc gcctgcttgt gcgcgagcac gcgcgcgcgg aacgcggcac
      361 gctcgcgcgc gtaggcttcc agtgtgtaca aatcggcagg attcaatttc tgcatttcag
      421 attccgtagg cacggcgtag caacccgatc ggactctccg ctggcggctg gtcgccaagc
      481 ccgtgcgcga tgtggctggc cgccatcggg cagtcgctgg cgaaatgcgc gggcgcgatc
      541 tgttcgacgc gtgtttcgac gggtttggcg atcttgcgcg agaacgcgta ggtcttggcc
      601 ttgacgccgt aggtgccgtc gtggccggag cagcgctcga tcgtctggat ctcggtgccg
      661 ggtaccagcg ccagcacgtc gcgcgtcttc gggccgatat tttgcacgcg ctggtggcag
      721 ggcgcgtggt aggcgacctt gccgagcgga ttcggaaatt gcgttttcag caggccggcc
      781 tggtgccgct gccacaggta ttcgaaggga tcgaagatgt ggcgcttcac cagctgcacg
      841 tcctcgtcgt ccgggaacat cagcggcagt tcctgcttga acatcagcac gcaggatgga
      901 atcgtcgcac tgatgtccca gccgtcgcgg atcgccgcgg cgagcacagg gatattttgc
      961 gttttatact tttccacgga tgccaggtcg cccagttcga gcttgggcat gccgcagcac
     1021 acctcgcgct ccacgagttt caccgcgacg ccgttgtggc gcagcacggc ggcgagatcc
     1081 tccacgggtt ccggctgcga gtaattgcag tagcaggtcg cgaagatcgc taccttgccg
     1141 cgcgtgcgtc cggccggctg cgcgtcgccc gtgccatcga ggttcttcag gcgcttgcgc
     1201 gccgtgcgcg cggcgtattg cggcacgcgc gcgtcgggat gcacgccagc gccttttcca
     1261 gcagcttgcg gccgagaccg ctgctgttga ccgcgttcac cgcttcgacg acgacgggaa
     1321 tcgtcgccaa tttgccgacc gcggtggtgg aggaaagaat cttggcgctc gtcttcgtct
     1381 cgccacgccg gtgcgcggtc gccttggcgc gcagcatcac gtgcggaaaa tccacgttcc
     1441 acggatgcgg cggcacgtac gggcacttgg tctggtagca caggtcgcac aggtaacact
     1501 ggtcgacgac cttcgcgtag tcggatttcg ccacgccatc gacttccatc gtggcggagt
     1561 tgtcgacgag gtcgaacagg gtcgggaacg cctggcacag gctgacgcag cgccggcagc
     1621 cgtggcagat gtggaacacg cgctccattt ccgcttccag cgccgcctgg tcgtagaact
     1681 gcgggttctt ccagtccagc ggatgccgcg tcggcgcatc gaggctgcct tcgcgggagg
     1741 tgctgcgttc gtcggccatg cgtggatcct tgccatggaa gcagcttcct cccgttcgga
     1801 aggaagctgt ggagcggctc aggcgagatc gttcaacgcc ttggtgaagc ggtttgcgtg
     1861 cgaacgctcg gccttggcca gcgtctcgaa ccagtcggcg acttcgccga agccttcttc
     1921 gcgcgcggtc ttggccatgc ccggatacat gtcggtgtat tcgtgggttt cgccggcgat
     1981 cgccgatttg aggttggcct tggtgtcgcc gaacggcagg ccggtggctg gatcgccgca
     2041 ctgttcgaga tattccaggt gcccgtgcgc gtgcccggtt tcgccttcgg ccgtggaacg
     2101 gaacacggcg gagacgtcgt tgtagccttc cacgtccgct ttctgggcga agtagagata
     2161 gcggcggttc gccatcgatt cgccgccgaa tgcatctttc agactttgct cggtcttgga
     2221 gcctttcaga gtggccatga cacacctcgt gttgtggtgg aaattcgagc ctgccgcatg
     2281 cgcggcggac ggggccagcg taacgcagtg gagcccgtct tgaaaatcga ttgttacgat
     2341 cgatccgata gagaaagtct atcgatgggc gcgccgtcgc ggcgcctgcg cgggcgatac
     2401 gacgacggcc ccgcgcgcat ccagcagctc tttcggcaac tcgcgaaaaa tctccgc
//
LOCUS       DASRVO010000383         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_63261, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000383 DASRVO010000000
VERSION     DASRVO010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63261"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="hflD"
                     /locus_tag="VFF05_04805"
     CDS             complement(<1..619)
                     /gene="hflD"
                     /locus_tag="VFF05_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HZW51158.1"
                     /translation="MREGRVIALAGMFQALALVRAIARRGSCDATIARHSLASVLRID
                     ADSPAAVYAGADNVRLGLETLVSQLDMTGKRDPTLTRMAVQVLRLERALMRHHKILSA
                     LRGGIDGMRALAPQVEAGNVDPSGRLAELYASTLSRLQPRIMVEGNPVFLQQSAQVDR
                     IRALLMAAVRAAVLWRQLGGNQWSLMFRRRQYAMLARGLLAACAIE"
     gene            complement(616..>1092)
                     /locus_tag="VFF05_04810"
     CDS             complement(616..>1092)
                     /locus_tag="VFF05_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HZW51159.1"
                     /translation="IPARRGEIRTPQGERIGEHDGTMYYTLGQRSGLGIGGRRDAGGE
                     PWYVVGKDVAANVLFVAQGGATQWLDSTRLTASELSWTAGTSPSGEFSCTAKTRYRQP
                     DQACRVQMRDDGYVVEFDLPQRAVTPGQSVVFYRDEECLGGGVIDATNAPYGGLPQ"
BASE COUNT          171 a          365 c          375 g          181 t
ORIGIN      
        1 cctcgatcgc gcaggccgcc agcaagccgc gcgcgagcat ggcgtattgg cggcggcgga
       61 acatcaggct ccactggttg ccgccgagct ggcgccagag cacggcggcg cgcacggcag
      121 ccatgagcag ggcgcggatg cggtcgacct gcgccgattg ctgcaggaag accggattgc
      181 cttcgaccat gatgcgcggc tgcaggcgcg agagcgtgct tgcgtacaat tcggccaggc
      241 gtccgctcgg atcgacgttg ccggcttcga cctgcggggc cagcgcacgc atgccgtcga
      301 ttccgccgcg cagagccgaa agaattttgt ggtggcgcat cagtgcgcgc tccaggcgca
      361 gcacctgaac ggccatgcgg gtgagggtgg gatcgcgctt gccggtcatg tcgagctgcg
      421 ataccagcgt ttccaatccc aggcgtacgt tgtcggcgcc ggcataaacg gcggcgggac
      481 tgtccgcgtc gatgcgcagg acgctcgcca gactgtggcg tgcgatcgtg gcgtcgcaac
      541 tgccgcgccg ggcaatggcg cgcaccagcg ccagcgcctg gaacatgccc gccaacgcaa
      601 tgacgcggcc ttctctcatt ggggcagtcc gccgtagggt gcattcgtcg cgtcgatcac
      661 gccgccaccc aggcattcct catctcggta gaacaccacg gattgccccg gcgttaccgc
      721 gcgctgcggc aggtcaaatt ccacgacata gccatcatcg cgcatttgca cgcggcaggc
      781 ctggtcgggt tgccggtagc gcgttttcgc ggtgcaggaa aattccccgg atggcgacgt
      841 gccggcggtc cagctcagtt cgctcgcggt caggcgcgtg gaatccagcc actgcgtcgc
      901 gccgccctgc gctacgaaga gaacattcgc ggcaacgtcc ttgccgacga cataccaggg
      961 ttcgccgccg gcatcgcggc gtccgccgat gcccaggccc gagcgttggc caagggtgta
     1021 gtacatcgtg ccgtcgtgct cgccgatgcg ctcgccttgc ggcgtgcgga tttcgccgcg
     1081 ccgggctgga at
//
LOCUS       DASRVO010000384         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_37957, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000384 DASRVO010000000
VERSION     DASRVO010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37957"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFF05_04815"
     CDS             complement(<1..190)
                     /locus_tag="VFF05_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZW51160.1"
                     /translation="MKTLSVKVKPNARASSLEQVGGVWFAQIKSPPVDGKANAELIAL
                     VAEKFGCRKNQVTIRAGAS"
     gene            complement(187..>1567)
                     /locus_tag="VFF05_04820"
     CDS             complement(187..>1567)
                     /locus_tag="VFF05_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HZW51161.1"
                     /translation="SKKETDEALAKLRDGKIDVMIGTHRLLQADVKFKELGLVVVDEE
                     HRFGVRHKEALKKLRAEVDLLTLTATPIPRTLNMAMSGLRDLSIIATPPEHRLAVKTF
                     VTPWDAATVREAFQRELGRGGQVYFLHNAVDSIERTARELGELIPDARIRIAHGQMPE
                     RELEQVMLDFYRQRFNVLVCTTIIESGIDVPSANTILIDRADRFGLAQLHQLRGRVGR
                     SHHRAYAYLIVPDKRALSADAEKRLDAIAAMEELGAGFTLATHDLEIRGAGELLGEEQ
                     SGQIEEIGFSLYSELLDRAVRALKEGKVPDFDLTSEHDAEVELHLPALIPDDYLADVH
                     ARLTLYKRIASARDADALRELQVEMIDRFGLLPDSVKNLFAAAALKLGANALGIRKIE
                     LGENGGRVVFKPNPSVDPLTVIRLIQSQPKTYALDGQDKLRIRMPLPGTAERLGAAQN
                     LLRTLAARP"
BASE COUNT          245 a          492 c          511 g          319 t
ORIGIN      
        1 cgctcgctcc agccctgatc gtcacctgat tcttgcggca gccgaatttt tccgcgacga
       61 gcgcaatcag ttccgcgttt gccttgccat ccaccggcgg cgacttgatt tgcgcgaacc
      121 acacaccgcc cacttgctcg agcgaactcg cgcgcgcatt cggtttgacc ttcaccgaca
      181 aggttttcat ggtcgcgcgg cgagcgtacg caacaggttc tgcgccgcgc cgagacgctc
      241 ggccgtaccg ggcaacggca tgcggatcct gagtttgtcc tggccgtcca gcgcataggt
      301 tttcggctgc gactggatca gtcggatcac ggtcagcgga tcgacgctcg gattcggctt
      361 gaacacgacg cgtccgccat tctcgccaag ttcgattttg cggatgccga gcgcgttcgc
      421 gccgagcttg agtgcggcgg cagcgaacag gtttttcacg ctatccggaa gcaaaccgaa
      481 ccggtcgatc atctccactt gcagttcgcg cagcgcatcc gcgtcgcgcg cgctggcgat
      541 gcgcttgtac agggtcaggc gcgcgtgtac gtcggcgagg tagtcgtcgg gtatcagcgc
      601 cggcaggtgc agttcgactt cggcatcgtg ctcgctggtg aggtcgaaat ccggcacctt
      661 gccttccttc agcgcgcgca cggcgcggtc gagcagttcg gagtaaaggc tgaacccgat
      721 ttcctcgatc tgtccggatt gttcctcgcc gagcagctcg ccggcgccgc ggatttccag
      781 gtcgtgggtg gccagggtga agccggcgcc gagttcctcc atcgcggcaa tggcatcgag
      841 gcgtttctcc gcgtccgcgc tcaaggcgcg cttgtcgggc acgatcaggt aggcgtaggc
      901 gcgatggtgc gaacgcccga cgcggccgcg caactggtgc aactgcgcca gaccgaaacg
      961 gtcggcgcgg tcgatcagga tggtgttcgc gctcggcacg tcgatgccgg attcgatgat
     1021 cgttgtgcac accagcacgt tgaaacgctg gcggtagaaa tccagcatca cctgttcgag
     1081 ctcgcgctcc ggcatctggc cgtgggcgat gcggatgcgc gcatcgggaa tcagttcgcc
     1141 gagttcgcgc gcggtgcgtt cgatgctgtc gacggcgttg tgcaggaagt acacctgccc
     1201 gccgcgtccg agttcgcgct ggaatgcctc gcgcaccgtg gccgcatccc agggcgtgac
     1261 gaaggttttc accgcgaggc gatgttcggg cggcgtggcg atgatggaca gatcgcgcag
     1321 gccggacatt gccatgttca gcgtgcgcgg gatcggcgtc gcggtgaggg tgagcagatc
     1381 gacttcggcg cgcagttttt tcagcgcctc cttgtgacgc acgccgaaac ggtgttcctc
     1441 atcgacgacg acgaggccca gttccttgaa cttcacgtcc gcctgcagca gccgatgcgt
     1501 gccgatcatc acgtcgatct tgccgtcgcg caatttggcc agggcttcgt cggtttcctt
     1561 cttcgac
//
LOCUS       DASRVO010000385         1296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_88672, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000385 DASRVO010000000
VERSION     DASRVO010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88672"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VFF05_04825"
     CDS             <1..1100
                     /locus_tag="VFF05_04825"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HZW51162.1"
                     /translation="FRLDQFRRDRSQRASSARSLAQRWASQVAATEGPARDDALPSTG
                     VMLAFAFPDRVARNRGNGGFVLANGRGAAVDQASALARAPYIAVAELTGTAAQGRILL
                     AAPIMQAEIEQHFADQIETADEISFDRGAMALRARRRKSLHALTLSEVPLALQPSAET
                     ARVLADGLVSAGLDKLPWTKSAKQWRDRVTFLRKAEGDTLQHSWPDLSDDALAAQREA
                     WLVPALYDKTSLKEFSSGDLSEALMTLLPWELRARLEREAPTHFEAPTGTMLAIDYEA
                     EQGPTIAVRLQELFGLNAHPSIAKGAIPLVLELLSPAQRPVQVTRDLPGFWRGSYAAV
                     RSDLRGRYPRHPWPEDPASALATRRVKPRGT"
BASE COUNT          206 a          412 c          444 g          234 t
ORIGIN      
        1 atttcaggct tgatcaattc cgccgcgacc gctcgcaacg cgcctccagc gcgcgctctc
       61 tcgcgcagcg ctgggcctcg caggtggcgg ccaccgaagg tccggcccgc gacgatgcgt
      121 taccctccac cggcgtgatg ctcgccttcg cattccccga ccgcgtcgcg cgcaatcgcg
      181 gcaatggcgg ttttgtgctt gccaacgggc gcggcgcggc ggtggatcag gcttccgcgc
      241 tggcgcgcgc gccctatatc gcggtcgccg aattgacggg taccgcggcg caggggcgca
      301 tcctgctggc ggcgccgatc atgcaggccg agatcgagca gcatttcgcc gatcagatcg
      361 agaccgcgga cgagatttcg ttcgatcgcg gcgcgatggc gctgcgggcg cgccgcagga
      421 agtcgctgca cgcgctcacg ctgtcggagg tcccgttggc gctgcagccg tcggcggaga
      481 ccgcgcgcgt gctggccgat gggctggtct cggctggtct cgataaattg ccttggacca
      541 aatcggcaaa acagtggcgt gaccgcgtga cgttcctgcg caaagccgag ggtgacactt
      601 tgcagcattc ctggcctgac ctttccgatg acgcgctggc ggcgcaacgc gaggcctggc
      661 tggtgccggc gctctacgac aagacttcgt tgaaggaatt ttcatccggc gatctgtccg
      721 aagcgttgat gacattgctg ccctgggaat tgcgcgcgcg gctggagcgc gaggcgccga
      781 cgcatttcga ggcgccgacc ggaaccatgc tcgcaatcga ctacgaggcc gaacaggggc
      841 cgacgatcgc ggtccgcttg caggaattgt tcgggctcaa cgcccatccg tcgattgcga
      901 aaggtgcgat tccgctggtc ctggaattgc tgtcgccggc acagcggccg gtgcaggtga
      961 cgcgcgattt gccgggcttc tggcgcggca gttacgcggc ggtccgatcc gatctgcgcg
     1021 ggcgctatcc ccggcatccc tggccggagg acccggccag cgcgctggcg acccggcggg
     1081 tgaagccgcg gggaacctga taccccaccg aatccggctc gaattgtgga tgacggaaat
     1141 ttgtcaccca ggtgaactct ttcgaaggcc atgcgttccg tttgcggaac cggccggtag
     1201 aggattgatc atgagcgctg cgtatatttt cgacgtcgaa ggcaccttga tcgactgcgc
     1261 ggcagagacc ctgcgatgct ggagcgaaac gctggc
//
LOCUS       DASRVO010000386         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_193879, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000386 DASRVO010000000
VERSION     DASRVO010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193879"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..311
                     /locus_tag="VFF05_04830"
     CDS             75..311
                     /locus_tag="VFF05_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZW51163.1"
                     /translation="MGEELTRRELEVAALVYDGFSDKAIAKRLGIAIRTVRKHIDAAG
                     AKLPPLPGECPGWAKPRPRIYHWWHEQGARGNAA"
     gene            363..935
                     /locus_tag="VFF05_04835"
     CDS             363..935
                     /locus_tag="VFF05_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HZW51164.1"
                     /translation="MSAKPTRATGGEGVLSEKRARFVAEYLVDLNATQAAIRAGYSER
                     TAYQQGSRLLSDVDIEAAIVAAQAERAERTQVTVDRVLLELARIGFSDISNYEVDDDG
                     NVTLVEGADPQAMRAVSTLKRKTRIIPQKNGEPIVEHDVEIKLWDKNTGLANCGKHLG
                     MFAENVNLRTPDGPLQVHITRRIVRPNGDR"
     gene            922..>1114
                     /locus_tag="VFF05_04840"
     CDS             922..>1114
                     /locus_tag="VFF05_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HZW51165.1"
                     /translation="MATVELDIPTPAWAEPLLAPMRYKGASGGRASGKSHFFAERAVE
                     EMVCDPSLRFACIREVQRSL"
BASE COUNT          215 a          352 c          380 g          167 t
ORIGIN      
        1 tggcgaggtc gtgagtacgg acatcgtgca ggactggagc gacgcagcgt agtgcctctg
       61 acgaaggagg acggatgggc gaggaactga cacggcgcga gctggaggtt gcggcgctcg
      121 tctacgacgg attctcagac aaggcgattg cgaagcggct cggcatcgcg atccgcaccg
      181 tacgcaagca catcgacgcg gccggcgcga agctgccgcc gttgcccgga gagtgtcccg
      241 gctgggcgaa accgcgcccc cgcatttacc actggtggca cgaacagggc gccaggggca
      301 acgccgcgta gtgttgttct gacccatacc cgccgcgtgg ccgaagtgcc acgtttcccg
      361 gcatgagtgc aaagccgacg cgggcgacag gcggcgaggg cgtgctttcg gagaagcgcg
      421 ctcgcttcgt tgcggaatac ctcgttgacc tgaacgcgac gcaggccgca atccgcgctg
      481 ggtacagcga gcgcacggcg tatcagcagg gctcgcggct gttgagcgat gttgatatcg
      541 aggcggccat cgtcgcggcg caggcggagc gcgccgagcg aacgcaagtg acggtggatc
      601 gcgtgcttct ggaactcgcc cgcatcgggt tctccgacat ctcgaattac gaagtggacg
      661 acgacggcaa cgtgacgttg gtcgaaggcg ctgacccgca ggcgatgcgc gccgtgtcca
      721 ccctcaagcg caagactcgc atcatcccgc agaagaacgg cgagcccatc gttgagcacg
      781 acgtagaaat caagctgtgg gacaagaaca ccggacttgc caactgcggc aagcacctcg
      841 gcatgttcgc cgaaaacgtg aacctccgca cgcccgacgg cccgctccaa gtccacatca
      901 cgcgccgcat cgttcgcccc aatggcgacc gttgaactcg acatccccac gccagcgtgg
      961 gccgagccgc tgttggcccc gatgcgctac aagggcgcaa gcggcggcag ggcgtcgggc
     1021 aagtcgcatt tcttcgcgga gcgggcagtc gaggaaatgg tgtgcgaccc gtcgctgcga
     1081 ttcgcctgca tccgcgaggt gcagcggtcg ctca
//
LOCUS       DASRVO010000387         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_38041, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000387 DASRVO010000000
VERSION     DASRVO010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38041"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..451
                     /locus_tag="VFF05_04845"
                     /pseudo
     CDS             1..451
                     /locus_tag="VFF05_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
     gene            complement(450..1235)
                     /locus_tag="VFF05_04850"
                     /pseudo
     CDS             complement(450..1235)
                     /locus_tag="VFF05_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1294..>2014)
                     /locus_tag="VFF05_04855"
     CDS             complement(1294..>2014)
                     /locus_tag="VFF05_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51166.1"
                     /translation="STTYYPTLPAISVAQGSNNIYIRLANDPNTFFSTNYALDPLTQS
                     DIFVLENFYNGSVQFDFNQAFTLTVDNLNSNDSNRFTDFAMPVYSPAQYPAALQPLPI
                     SGYLTGAWYDPNHSGEGIQVEVGEQGTPGTANDRYIVLAWYTYDTSGVPYWLYGEGNF
                     TSGDRQALVTMVYSTGGGFAGNFGSATTPTWGTINVYFTDCNTMQFSYQSASGLPTGV
                     PTGNGAKTWTRISQMNGLTCQ"
BASE COUNT          336 a          612 c          680 g          386 t
ORIGIN      
        1 cgtggccgag tcgagcgtga ccctgcccgc cattcggtcc gtgatcgata ccgggcaggc
       61 gcgcgagccg cgcttcgatc cgaattcggg tttctcgcgc ctgcagactg tggcgatttc
      121 ccaagcctct gcggatcagc gcgcgggccg cgcgggccgc gttgccgcag gcgtggccta
      181 ccggctgtgg ccgcaaagcc agcgcctgga gccatcgcgg cgcgcggaaa tcgcgcaggt
      241 ggaattgtcc gcgctggcgc tggaactttc ggcgtggggc tcgcccgacc tgcgctggct
      301 ggatgcgcca ccgtccggtg cgctggccag cgcgcgcgac ctgctggtcc tgctcggtgc
      361 gctcgacacg gatggccacg tcacgaaact cggccgcgac atgctcgctg ccggcgcctc
      421 gccgcgactg gccgcgatgg tgctgcgcgc gccggtgcga gtgcggcgtg ctgttcggcc
      481 aaggacagtt cgccgtgcag tgcaacgagt tcggcggacg catgcccctg caggatccgg
      541 cgagcctgtt cgatctcgcg tcgccccggc agaaacacca gaatgtcgcc gtcggtttcg
      601 cgcaatgcgg tttcgacaac gcggcgcaga tggaacggcc attcttcgtt cgccttggcg
      661 ggcggatatt cgatgcgcac cggaaaactt cgtccggcgg aagtgattct cggcgcatcg
      721 aaccagcgcg cgatgcgttc gccgtccagc gtcgccgaca tgatgacgag gcgcaggtcc
      781 gggcgcagtt gcgcctgcac gtcgagcgcg agcgcggcgc cgaggtcgcc gtgcaggtgg
      841 cgctcgtgga attcgtcgaa caggatcgcg ccgacgccgg acaattccgg atcggactgg
      901 atcagtcgcg tcaggattcc ctcggtcacg acttccacgc gcgtgcgcgc cgaaaccttg
      961 gcctcgaaac ggatgcgata gccaacggtt tcgccgaccg cttcgccgag ctgcgcggcc
     1021 atgaattccg ctgccgcgcg cgcggcgatg cggcgcggtt ccagcacgag gatttttttg
     1081 ccggcaagcc atttcgcgtc gagaagggcc agcggcacct gcgtggtctt gcccgcgccg
     1141 ggcggtgctt cgagcacgag gcgcggacgc agcgcgaggc tcgccagaat gtccggcaat
     1201 acttccagaa tcggaaattg tgtacttctt gtcatcgtat aaacgacgat cccgccacgc
     1261 gggcgggatc gaagcgttgc gtgtggaaac agtttactgg caagtcagcc cgttcatctg
     1321 gctgatgcgg gtccatgttt tcgcgccgtt gccagttggc acacccgtcg gcaagccgct
     1381 cgccgattga tagctgaact gcatcgtgtt gcagtctgtg aaatagacgt tgatcgtccc
     1441 ccaagtaggt gtcgtcgccg acccgaaatt gccggcaaag ccgccacccg tgctgtacac
     1501 catggttacc agggcttgac gatcgcccga ggtaaagttg ccctctccgt acagccagta
     1561 cggcacgccg gaagtgtcgt aggtgtacca ggccaatacg atatagcggt cgttcgccgt
     1621 gccgggcgtg ccctgttcgc cgacctcgac ctggatgcct tcgccgctgt gatttgggtc
     1681 gtaccaggcg ccggtgaggt agccgctgat cggcagcggc tgcaaagcgg cggggtattg
     1741 tgccgggctg tataccggca tggcaaaatc ggtgaatcgg ttcgaatcgt tgctattcaa
     1801 attgtcgacg gtgagcgtga aggcctggtt gaaatcgaac tgcacgctgc cgttgtagaa
     1861 attctcgagc acgaagatat cgctctgggt cagcggatcg agcgcatagt tggtggaaaa
     1921 gaacgtgttg ggatcgttgg ccagacggat gtagatgtta ttgctaccct gggccaccga
     1981 aatggcggga agtgtcgggt agtaagtcgt gctg
//
LOCUS       DASRVO010000388         1292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_271494, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000388 DASRVO010000000
VERSION     DASRVO010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271494"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFF05_04860"
     CDS             complement(<1..573)
                     /locus_tag="VFF05_04860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZW51167.1"
                     /translation="MHDAMAAQSTDGNANRARRIALILLIVVYTLNFVDRQILSILKE
                     PIAADLHLSDTDLGLLGGFAFALFYSLLAIPFAWLADRFSRVWIISAGLFLWSGFTAM
                     CGMAGSFAVLFLARMGVGVGEAGGVAPAYSLIADWFPPRSRARALAAYSFGIPIGSAL
                     GVLFGGLVAKAVDWRFAFIAIGAFGVLLTPL"
     gene            complement(628..>1292)
                     /locus_tag="VFF05_04865"
     CDS             complement(628..>1292)
                     /locus_tag="VFF05_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZW51168.1"
                     /translation="IADEVLDTFEFGSKSSFADGRFALNADVFYTRYRDIQLSVFTSF
                     TQANGTPGFYGDYTNAGKAHAYGIEAEFAWRPIEHWSLIGNVAGLHTQYDEYFTNGVN
                     IADTQRFPAAPNGQAGLNLEYSSPVDFGGNIRARVGYTYQTKEFPTTDLSPAIAQGPY
                     GLLGATLIWNRDAHWTFALRGSNLTNKSYRTDGYNIPALGVLTGFYGAPRLVFASVKY
                     AF"
BASE COUNT          259 a          406 c          432 g          195 t
ORIGIN      
        1 caatggcgtc aacagcacgc cgaacgcgcc gatcgcaatg aaggcgaagc gccaatcgac
       61 cgccttggcg acgaggccgc cgaacagcac gcccagggcg ctgccgatcg ggatcccgaa
      121 cgagtacgcg gccagcgcgc gcgcgcgcga acgcggcgga aaccagtccg cgatcaggct
      181 gtacgccgga gcgacgccgc cggcttcgcc gacgcccaca cccatgcgcg ccaggaacag
      241 cacggcgaag ctgcccgcca tgccacacat cgcggtaaag cccgaccaca ggaacaaacc
      301 cgcggagatg atccacacgc gcgaaaagcg gtccgcgagc caggcgaacg gaatcgcgag
      361 cagcgaatag aacaatgcga acgcgaagcc gccgagcagg ccaagatcgg tgtccgacaa
      421 atgcaggtcg gcggcgatcg gttccttgag gatggaaagg atttgccgat cgacgaaatt
      481 cagcgtgtag acgacgatca gcaggatcag cgcgatccga cgcgcgcgat ttgcgttccc
      541 atccgtcgat tgcgccgcca tggcgtcatg catgcgcatt tcccgttttc ccggcggccg
      601 ccctgacgca gccgccggga agcgcgatca gaacgcgtac ttcacgctgg cgaacaccag
      661 ccgcggcgcg ccgtagaaac cggtcagcac gcccaatgcc ggaatgttgt agccatcggt
      721 gcggtacgac ttgttcgtca ggttgctgcc ccgcagggca aacgtccagt gtgcgtcgcg
      781 gttccagatg agcgtggcgc cgagcaggcc gtaggggccc tgcgcgatcg cgggactgag
      841 gtcggtggtc gggaattcct tggtctggta ggtgtagccg acgcgcgcgc ggatattgcc
      901 gccgaaatcc accggagagg aatactcgag gttgaggccg gcctgtccgt tcggcgcggc
      961 ggggaaacgc tgcgtgtcgg cgatgttcac gccgttcgta aagtactcgt cgtactgcgt
     1021 gtgcaatccg gcgacgttgc cgatcagcga ccagtgttcg atcgggcgcc aggcgaactc
     1081 ggcctcgatg ccgtaggcgt gcgccttgcc ggcgttggtg tagtcgccgt agaaccccgg
     1141 cgtgccattg gcctgcgtga acgaggtgaa caccgacaac tggatgtcgc ggtagcgcgt
     1201 atagaacacg tcggcgttga gcgcgaagcg gccatcggca aaactcgatt tgctgccgaa
     1261 ctcgaacgtg tcgagcactt catcggcgat cg
//
LOCUS       DASRVO010000389         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_348603, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000389 DASRVO010000000
VERSION     DASRVO010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348603"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1296
                     /gene="dusA"
                     /locus_tag="VFF05_04870"
     CDS             280..1296
                     /gene="dusA"
                     /locus_tag="VFF05_04870"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017355068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HZW51169.1"
                     /translation="MADIVRSKNVNVPQAWRLCVAPMMDWTDTHCRVFHRLLSPHARL
                     YTEMVHAHAVVRGDRARLLGFDRCEHPVALQLGGHEPAELATAARIGVEFGYDEINLN
                     VGCPSDRVQSGRFGACLMREPALVADCVAAMRAAVGVPVTVKCRIGVDAQDDYEDLHR
                     FAETLIGAGLEVLIVHARKAWLHGLSPKENREVPPLNYPRVYRLKQEFPRLIVVINGG
                     IVDADASKSHLQNVDGVMLGRAAYHDPYVLARVEHALFDSPLPLRDDVLRALEPYIVA
                     HLRRGEVLHHIVRHVLGLYLGEPGARAFRRHLGEHMRAAGADFRTVEAAQASLREHAR
                     ARAA"
     gene            1350..1586
                     /locus_tag="VFF05_04875"
     CDS             1350..1586
                     /locus_tag="VFF05_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HZW51170.1"
                     /translation="MQRRLAIAALFAALFGAGTAWAGQITLQQAIEKVEHETRGKVLS
                     AETKHYGKHTIYRIKVLTKNGQVRVIEVPAEQSD"
     gene            1616..2326
                     /locus_tag="VFF05_04880"
     CDS             1616..2326
                     /locus_tag="VFF05_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZW51171.1"
                     /translation="MRILLVEDEAPLRETLAARLKREGYAVDMAADGDEGLYLGREVP
                     FDVAIIDLGLPKKSGMELINDLRAAGQRCPILILTARTSWQDKVTGLKQGADDYLVKP
                     FHVEELLARINALVRRASGWTKPVLECGPIVLDTTAQMVKVDGQAADLTSYEYKVLEF
                     LILHAGELVSKADLTEHIYQQDFDRDSNVLEVFIGRLRRKLDPDNTLKPIETVRGRGY
                     RFAIPRSDNTPAPPPAAA"
     gene            2337..>2608
                     /locus_tag="VFF05_04885"
     CDS             2337..>2608
                     /locus_tag="VFF05_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HZW51172.1"
                     /translation="MAQRRPLSIQARSLLAAGVALAAFLGLTGFALDQAVYETLRSAV
                     RDRLQSYVYGYLAASDVARSGRWLPPDVSPDPRFDRPNGGLYAGVAG"
BASE COUNT          440 a          858 c          865 g          445 t
ORIGIN      
        1 gtcgtgcatg cgcgtcgtga agccgacgcg gacgcgattg ccgcgcgcgt gcgggaagcc
       61 ttcgtgctcg gcgcggaggc gcccgccagc gtgccgccgt ggcgctggct ggattgagcc
      121 gggccgccgc gccgcgttgc ccgccgccac gacgcctcag taccggcgat ccccagaggc
      181 cagcatggtt gcgcgtcgaa gcgaggtaag gcattcgccg cgtcgatcat ggatacaatg
      241 tgggttcggc cgcaacccga cgcggctcag tagtttccga tggcggatat cgtccggtcg
      301 aaaaacgtga acgtgccgca agcctggcgc ctgtgcgtcg cgccgatgat ggattggacg
      361 gacacgcact gccgcgtgtt ccatcgtctg ctcagcccgc atgcgcgttt gtataccgaa
      421 atggtgcacg cgcacgccgt ggtgcgcggc gatcgcgcgc gcctgctcgg cttcgatcga
      481 tgcgagcatc cggtcgcgct gcagctcggt gggcatgagc cggcggagct cgcgacggcg
      541 gcgcgcattg gcgtggaatt cggctacgac gaaatcaacc tcaatgtcgg ctgtccgtcg
      601 gatcgcgtgc aatccgggcg cttcggcgcc tgcctgatgc gcgagccggc actggttgcc
      661 gattgcgtgg cggcgatgcg cgcggcggtc ggcgtgccgg tcacggtcaa atgccgcatc
      721 ggcgtggacg cgcaggacga ttacgaggat ttgcaccgct tcgccgaaac gctgatcggc
      781 gccgggctcg aagtgctgat cgtgcatgcg cgcaaggcct ggctgcacgg cttgagcccg
      841 aaggaaaacc gcgaggttcc gccgctcaac tatccgcgcg tgtatcgcct gaagcaggaa
      901 tttccgcgat tgattgtcgt catcaacggc ggcatcgtcg atgccgatgc ttcaaaatcc
      961 catttgcaaa atgtagacgg cgtcatgctc ggccgtgcgg cgtatcacga tccttacgtg
     1021 ctggcgcgcg tcgagcacgc gctcttcgac tcgccgttgc cgctgcgcga tgacgtactg
     1081 cgcgcgctgg aaccctacat cgtcgcgcat ctgcgccgtg gcgaggtgtt gcatcacatc
     1141 gtccgccatg tgctgggcct gtacctgggc gagccgggcg cgcgcgcgtt ccggcgtcac
     1201 ctgggcgaac acatgcgcgc ggcaggcgcg gatttccgca ccgtcgaggc cgcgcaggcg
     1261 tccctgcgcg agcacgcgcg ggcgcgagcg gcttagcacg gcgccgcgat tcaggtggga
     1321 ttacacggtc gtcgcgttat gatgcgggca tgcaacgccg actcgccatc gccgccctgt
     1381 ttgccgcctt gttcggggcg ggtacggcgt gggcgggaca gatcaccttg cagcaggcga
     1441 tcgagaaggt cgaacacgaa acccgcggca aggtgctgtc ggcggaaacc aaacattacg
     1501 gcaagcacac gatttaccgc atcaaggtgt tgaccaaaaa cggccaggtg cgtgtgatcg
     1561 aggtgccggc cgagcaatcg gattagtccg taaaaatcaa cgcaaggaat cgaacatgcg
     1621 cattcttttg gtcgaagacg aagcgccgct gcgcgagacg ttggccgcgc gcctgaaacg
     1681 cgaaggttat gccgtggaca tggcggccga cggcgacgaa ggcttgtacc tcggccgcga
     1741 ggtgccgttc gacgtcgcca tcatcgacct cggcctgccg aagaaatccg gcatggaatt
     1801 gatcaacgac ctgcgtgcgg ccggacagcg ctgcccgatc ctgatcctca ccgcgcgcac
     1861 gagctggcag gacaaggtca ccgggctcaa gcagggtgcc gacgattacc tggtcaaacc
     1921 cttccacgtc gaggaattgc ttgcgcgcat caacgcgctg gtgcgccgcg ccagcggctg
     1981 gacgaaaccc gtcctcgaat gcgggccgat cgtgctcgac accacggcgc aaatggtcaa
     2041 ggtcgacggt caggccgcgg acctgaccag ctacgaatac aaggtgctgg aattcctgat
     2101 cctgcacgcc ggcgaactcg tgtccaaggc cgacctgacc gagcacatct accagcagga
     2161 tttcgaccgc gactcgaacg tgctcgaggt cttcatcggc cgcctgcgtc gcaagctcga
     2221 tccagacaat acgctcaagc cgatcgaaac cgtacgcggg cgcggctacc gcttcgccat
     2281 tccgcgttcg gacaacacgc ccgcgccgcc gccggccgcc gcctgaccct tcgccaatgg
     2341 cgcagcgccg cccgctgtcg atccaggcgc gctcgctgct cgccgccggc gtcgcgctgg
     2401 cggcatttct cggcttgacc ggctttgccc tggaccaggc cgtctacgaa accctgcgca
     2461 gcgccgtgcg cgatcgtttg caaagctatg tctacggata tctggctgcg tcggacgtgg
     2521 cgcgctcggg acgctggctg ccgccggacg tctcgcccga tccgcgtttc gaccgcccca
     2581 acggcggact ctacgccggc gtcgccgg
//
LOCUS       DASRVO010000390         1129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_11585, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000390 DASRVO010000000
VERSION     DASRVO010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11585"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..789
                     /gene="mtgA"
                     /locus_tag="VFF05_04890"
     CDS             64..789
                     /gene="mtgA"
                     /locus_tag="VFF05_04890"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZW51173.1"
                     /translation="MKSGKSNRAHGLLCRLWRWAWRLGVAWVVLTWLVVVLLRFVPPP
                     TSAFMLERRIAAWHAGEKAFVLRYRWAPWRDISPQLPIALIASEDQKFPFHHGFDVQA
                     IQDALDEAEDGERLRGASTISQQTAKNLFLWSGRSFVRKGLEAYFTVLLEITWPKRRI
                     LEVYMNIAEFGNGIYGADAAARTFFHEAPAQLDAYQCALLASVLPNPKKLHADRPSPY
                     VLSHAEWTQRQVRQLGGVGYLPR"
     gene            complement(794..>1129)
                     /locus_tag="VFF05_04895"
     CDS             complement(794..>1129)
                     /locus_tag="VFF05_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HZW51174.1"
                     /translation="VLLFAFQGEDIMRQPLVIALLAVPITIQVVFNAALAYGLNRLGG
                     EAHCVAGPSALIGASNFFELAVATAIALFGFQSGAALATVVGVLIEVPLMLGVVAVVN
                     RSRVWYEKR"
BASE COUNT          215 a          345 c          367 g          202 t
ORIGIN      
        1 cgcgaaaatc aacatcgatc cggactattg cacatcgccg cggcctcggc catcttgcgc
       61 ggcatgaagt cgggtaaatc gaatcgcgcg catggcttgc tgtgccgact gtggcgttgg
      121 gcctggcgtc tcggggtggc gtgggtcgtg ctgacatggc ttgtcgtcgt gctgttgcgc
      181 ttcgtgccgc cgccgacctc ggcgttcatg ctcgagcgtc gcatcgcggc ctggcatgcg
      241 ggcgagaagg ccttcgtgtt gcgttaccgc tgggcgccgt ggcgcgacat ttcaccgcag
      301 ttgccgatcg cactgatcgc cagcgaagac cagaaattcc cgtttcatca cggcttcgac
      361 gtacaggcga tccaggacgc gctcgacgag gccgaagacg gcgaacgcct gcgcggggcg
      421 agcacgatca gccagcagac cgcgaagaac ctgttcctgt ggagcgggcg cagcttcgtg
      481 cgcaaggggc tggaggcgta tttcacggtg ctgctggaaa tcacctggcc caagcgccgc
      541 atcctcgagg tgtacatgaa catcgccgaa ttcggcaacg gcatctacgg tgccgacgcg
      601 gcggcgcgca cgtttttcca tgaggcaccg gcgcaactcg acgcgtatca atgcgcattg
      661 ctggcttcgg tgctgccgaa tccgaaaaag ctgcatgcgg atcgcccatc gccgtatgta
      721 ttgagccatg cggagtggac gcagcgccag gtgcggcaat tgggcggagt cggctatttg
      781 ccgcgttgag acgctagcgt ttctcgtacc agacccggct gcgattgacc acggcgacga
      841 cgccgagcat caggggcact tcaatgagaa cacccacgac tgtcgccagc gcggcgccgg
      901 attgaaagcc gaacagcgca atggccgtgg cgaccgccaa ctcgaaaaaa ttgctggctc
      961 cgatcaaggc ggagggtccc gccacgcaat gggcttcgcc gcccaggcga tttaggccgt
     1021 aggcgagcgc tgcattgaat acgacctgga tggtgatcgg taccgccagc agcgcgatga
     1081 ccaacggttg ccgcatgatg tcctcaccct ggaaagcgaa caacagcac
//
LOCUS       DASRVO010000391         1419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_194019, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000391 DASRVO010000000
VERSION     DASRVO010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194019"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1419
                     /locus_tag="VFF05_04900"
     CDS             <1..>1419
                     /locus_tag="VFF05_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZW51175.1"
                     /translation="TDTFTQMGGEGATWCGQAPFTETKHVFQNLGDGTYFHSGSLAIR
                     QAVAAKVNITYKILYNDAVAMTGGQPVDGKLSVPDIAHQVRSEGVRTIIIMSDDIEKW
                     SDASIFPSDVEFIHRDELESVQKRLREIEGVSALIYDQTCATEKRRRRKRGKLEDPKK
                     RVFINSLVCEGCGDCGKESFCVSVLPKETEYGRKREIDQSGCNKDFSCVKGFCPSFVT
                     VHGGGLRKRKPGSGKVDFFTLPQPQFASDLSQPWNILVTGIGGTGVVTIGALIGMAAH
                     LEGKGGTVLDQTGLAQKGGAVTCHLRIAKAPADIHAVRIAAGEADVVLGCDMVVVNDY
                     WSLSKIRAGRSHVVLNSYEAMPGTFTTKPDLRFPASGIVDAIKTALGGEKPELVDATD
                     LAVKLLGDSIGANLFMLGYAWQKGWVPLSLEALMRAVELNGAAVEMNKSAFNWGRMAA
                     HDIARVREAAGGKPVTSGGIPAT"
BASE COUNT          281 a          435 c          464 g          239 t
ORIGIN      
        1 accgacacct tcacgcagat gggcggcgaa ggcgcgacct ggtgcgggca ggcgccgttc
       61 accgaaacca aacacgtgtt ccagaacctc ggcgacggca cgtatttcca ttccggttcg
      121 ctcgcgatcc ggcaggcggt cgcggccaag gtcaacatca cctacaagat cctctacaac
      181 gatgcggtgg cgatgaccgg cggccagccg gtggacggca agctcagcgt gcccgacatc
      241 gcgcatcagg tgcgcagcga aggcgtgcgg acgatcatca tcatgtccga cgacatcgag
      301 aagtggtcgg atgcgtcgat cttcccgagc gacgtcgaat tcatccaccg cgacgaactc
      361 gaatccgtgc agaagcggct gcgtgaaatc gaaggcgtgt cggcgctgat ctacgaccag
      421 acctgcgcca ccgaaaagcg ccgtcggcgc aagcgcggaa aactcgaaga cccgaagaag
      481 cgcgtgttca tcaattcgct ggtctgtgaa ggctgcggcg actgcggcaa ggaatcgttc
      541 tgcgtgtcgg ttttgcccaa ggaaaccgaa tacggacgca agcgcgagat cgaccagtcg
      601 ggctgcaaca aggatttttc ctgcgtgaag gggttctgcc cgagcttcgt caccgtgcac
      661 ggcggtggct tgaggaaacg caagcccggc agcggcaaag tggatttttt cacattgccg
      721 caaccgcaat tcgcgagcga tctgtcccag ccctggaaca tccttgtcac cggcatcggc
      781 ggcaccggtg tcgtcaccat cggcgcgctg atcggcatgg ccgcacacct ggaaggcaag
      841 ggcggcaccg tgctcgacca gaccgggctc gcccagaagg gcggcgcggt gacctgccac
      901 ctgcgcatcg cgaaagcgcc tgcggacatc catgccgtgc gcatcgcggc gggcgaggcc
      961 gacgtggtgc tcggctgcga catggtcgtg gtcaacgatt actggtcgct gtcgaagatc
     1021 cgtgccgggc gttcgcacgt ggtgctcaat tcctacgagg caatgccggg cacgttcacg
     1081 acgaaacccg acctgcgctt tccggccagc ggcatcgtcg atgcgatcaa gactgcgctc
     1141 ggaggcgaaa agcccgaact cgtcgatgcc acggatcttg ccgtgaaatt gctcggcgat
     1201 tccatcggcg ccaacctttt catgctcggc tacgcctggc agaagggctg ggtgccgctg
     1261 tcgctggaag ccttgatgcg cgccgtggaa ttgaacggtg cggcggtgga gatgaacaag
     1321 tccgcgttca actggggccg catggcggcg cacgatattg cacgcgtgcg cgaggcggcc
     1381 ggtggcaagc cagtgacaag tggcggtatt cccgcgacg
//
LOCUS       DASRVO010000392         1068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_166355, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000392 DASRVO010000000
VERSION     DASRVO010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166355"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFF05_04905"
     CDS             complement(<1..501)
                     /locus_tag="VFF05_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HZW51176.1"
                     /translation="MTVRTRFAPSPTGFLHIGGARTALYCWLEARHRGGQFILRIEDT
                     DRERSTDEAVKAILDGMSWLGLDHDEGPFYQTLRMDRYRHVAEELVRAGKAYYAYETK
                     EELDAMRARQMEQNIKPRYAGVYREQNAPLRDDPNRVIRFRNPASGSVVFNDKVKGRI
                     EWSNDEL"
     gene            581..>1068
                     /locus_tag="VFF05_04910"
     CDS             581..>1068
                     /locus_tag="VFF05_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HZW51177.1"
                     /translation="MKRIALLLACTLALAACASMSQRPADGTRAQIGVLETTDLHSNV
                     MSYDYYKLAPDASLGLERAATLIHAARKEFPNTLLFDAGDTIQGTALADYQALVHKPA
                     CDQRLAIYKAMDTLHYDAGTIGNHEFNYGLPFLSQVTGTSFDATGVPVEHCKGPDFPL
                     VLA"
BASE COUNT          204 a          359 c          313 g          192 t
ORIGIN      
        1 gagttcgtca ttgctccact cgatgcgacc cttgaccttg tcgttgaaca cgaccgagcc
       61 cgacgccgga ttgcgaaaac ggatcacccg gttcgggtcg tcgcgcagcg gcgcgttctg
      121 ctcgcggtag acgccggcgt aacgcggctt gatgttctgc tccatctgcc gagcgcgcat
      181 cgcatcgagt tcttccttcg tctcgtacgc gtaatacgcc ttgcccgcac gcaccagctc
      241 ctcggcgacg tggcgataac gatccatgcg cagagtctgg tagaacgggc cttcgtcgtg
      301 atccaggccg agccagctca tgccgtcgag aatggccttc accgcctcgt cggtggaacg
      361 ctcgcgatcg gtatcctcga tgcgcaggat gaactggccg ccgcgatgac gcgcctccag
      421 ccagcagtaa agcgcggtgc gcgcgccgcc gatgtggagg aaaccggtag gactgggagc
      481 gaagcgtgtg cgaacggtca ttgtaaaaac tgtcgtgaaa cggcctgcta gcctagcaga
      541 tgcgcgataa ttccggacca attccctgcc ccgccatgcc atgaaacgaa tcgcactcct
      601 gctcgcctgc acgctcgccc tcgccgcctg cgcatcgatg tcgcagcgcc cggccgacgg
      661 cacgcgcgcg caaatcggcg tactcgaaac caccgatctg cattccaacg tgatgagcta
      721 cgactattac aagctcgcgc cggatgcgag cctgggcctg gaacgcgcgg ctacgctgat
      781 ccacgccgcg cgcaaagaat ttccgaatac gttgctgttc gacgccggcg acaccatcca
      841 gggcacggcg ctggccgatt accaggcgct cgtgcacaaa ccggcctgcg accagaggct
      901 cgcgatctac aaggcgatgg acacgctgca ctacgacgcc ggcaccatcg gcaatcacga
      961 attcaattac ggactgcctt tcctcagcca ggtgaccggc acatcgttcg acgcgaccgg
     1021 cgttccggtc gaacactgca agggtccgga ttttccgctc gtgctggc
//
LOCUS       DASRVO010000393         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_88937, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000393 DASRVO010000000
VERSION     DASRVO010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88937"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..594)
                     /locus_tag="VFF05_04915"
     CDS             complement(28..594)
                     /locus_tag="VFF05_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51178.1"
                     /translation="MADMYNAWNLRPAPMHDANPIAHHGKGERNVVAMGANVLPRYVG
                     TEFLIAIRLTVEYQAYLPALPAITELSSAKEDAELERHVEPRQPIARVKRGPREIVDS
                     ELALANDRVQFVDTHLGAVIQFQSASRDETAVVHRENESAQELLVPSVERNVDEDRIG
                     WTHRARTCLTLSYGGLLLSGWSPGCAAP"
     gene            complement(793..>2748)
                     /locus_tag="VFF05_04920"
     CDS             complement(793..>2748)
                     /locus_tag="VFF05_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZW51179.1"
                     /translation="HRAFGSDPSVVGRSVLLNGQATQIVGVLPADFRVPDGSRMILAD
                     VWRPLRFTPQAAAVRDNYPLETFGRLAPHATVRSAQLELRGIWTNLARAYPELRGSSV
                     RVAPLQAENVAPLRAPLALMLGAVALVLGIAVTNVSALLLARGVHRRREVAVRVALGA
                     SAWDSTRDAVAESLVLVAWGLALGVGLAFAAVRTVGVLAADELPQFDGLRLDLRVLAF
                     AVCVAVAAAALCAVAPALRARRVDPQDALRGARGAGAGREQHRALRGLVVAEIALSLV
                     LLLGAGLVLRGFTGLLSSDPGFDASHILRLYVSVVRSDAKRGNTMDAVVQPALEAIQR
                     VPGVEAAGVISSVPFVSWGNNMPVRYRWSSQTDEGQLPAAEIRRVTPGFFDVTRQRLI
                     AGRLLRNTDDEHSPPAYVVNEALVRRDFQGRDPIGASLYSYNRGAFGTIVGVVSNIKN
                     YGPLAAPVPEVYSTVQQDGIGSTLPLMIRTRVADPASVLSGVRAAIASVDPTAAISDA
                     LPMRDVIARSVGGPRFYANLLACFAAAAVLLAMAGMYGVLSYTVSQRTREIGVRAALG
                     GTPGRIMRLIVGEGLTLGATGAVLGAATGLAATRVMSSLLYGTSAIDPAAWVSAMLTL
                     LLVVAIATAIPAFRASRVAPAVALKTE"
BASE COUNT          516 a          953 c          863 g          416 t
ORIGIN      
        1 tcggccgcca ctcgcacggc gaggtcgtca cggagccgca caaccaggcg accagcccga
       61 cagaagaagg ccgccataag atagcgttag acacgtacga gcgcgatgcg tccatccgat
      121 tcggtcttca tcaacgttcc gttcgaccga cggtacaaga agctcttggg cgctctcgtt
      181 ttcgcggtgc accactgcgg tctcgtcgcg cgatgcgctc tggaattgga tgacggcgcc
      241 caagtgcgtg tcgacaaact gtacgcggtc attcgccagt gcgagttcgg aatccacgat
      301 ctctcgcgga ccacgcttga ctcgcgcaat cggttgccgc ggttcaacat gccgctcgag
      361 ctcggcatct tccttggcgc tcgacagttc ggtgatcgcg ggcaacgcag gaaggtatgc
      421 ctgatactcg accgtgagcc gtatcgctat cagaaattct gttccgacat agcggggcag
      481 gacattcgcg cccatcgcaa cgacgttgcg ctcgcccttg ccgtggtgcg cgattggatt
      541 cgcgtcatgc atcggcgcgg ggcgcaggtt ccaggcgttg tacatatccg ccaacgatat
      601 ctcatctttc agaaagagct gacgcgcatg tgccgcatcg cgaagctcga tcgaagcgag
      661 ttaggctttg tcgactacag aacgctcgta gcgggatggt tgagcgagaa tctatgacta
      721 gatccatgtt cgccagctcg acggcctcga ccaggccaga ttcgagtgct accgttgaac
      781 gtgaagaacg ggctactccg tcttgagcgc caccgccgga gcaacccgcg acgcgcggaa
      841 ggcaggaatc gcggtcgcga tggccacgac cagcaacagg gtcaacatcg cgctcaccca
      901 ggcggctgga tcgatcgcgc tcgtaccata gagcagggaa gacatcacgc gggtggcggc
      961 caatccggtc gcggcaccca gcaccgcgcc tgtcgctccc agagtcaacc cctcgcccac
     1021 gatcaatcgc atgatgcggc ctggcgtccc gcctaacgct gcgcggacgc cgatctcacg
     1081 cgtgcgctgc gagacggtgt aactcaacac gccgtacatt ccggccatcg ccagcagcac
     1141 ggcggccgcg gcaaagcacg ccagcaggtt ggcgtagaaa cgcgggccgc ccacactccg
     1201 cgcgatcacg tcgcgcatcg gcaacgcgtc ggagatcgct gccgtggggt cgacgctcgc
     1261 gatcgcggcg cgtacgccgg agagcacgct cgcggggtcc gccacacgcg tccgaatcat
     1321 cagcgggagg gtggacccga tgccatcctg ttgcaccgtc gagtagacct cgggcaccgg
     1381 cgctgccagc ggtccgtagt tcttgatgtt gctcaccacg ccgacgatgg tcccgaacgc
     1441 accgcggttg tacgagtaca agcttgcgcc gatcggatct cgcccctgaa agtctctgcg
     1501 cacgagcgcc tcgttcacca cgtacgcagg cggcgaatgc tcgtcgtcag tatttcgtag
     1561 tagtcttccg gcgatgagcc gctgacgggt cacgtcgaag aatcccggcg tgactctccg
     1621 aatctcggct gcgggcagct ggccctcatc cgtctgcgat gaccaacggt accgcacggg
     1681 catgttgttg ccccaggaca cgaacggcac gctgctgatc acgcccgcgg cttcaacgcc
     1741 gggcacgcgc tgaatcgcct cgagcgccgg ctgcacgacg gcgtccatcg tgttgccgcg
     1801 tttcgcgtcg ctgcgcacga cgctcacgta cagcctcaga atgtgcgacg catcgaagcc
     1861 gggatcgctc gacaacagtc cggtgaatcc gcgcagcacc aggccggcgc ctaacaacaa
     1921 taccagggac agcgcaatct cggcgaccac gagcccgcgc agcgcgcggt gctgctcgcg
     1981 cccggctccc gcgccgcgag caccgcgcag cgcatcctgc ggatccaccc gccgcgcgcg
     2041 aagcgccggc gccaccgcgc acagggccgc cgcggccacc gcgacacaca cggcaaaggc
     2101 gagcacgcgc aggtccaggc gcagaccgtc gaactgcggc agctcgtcgg cggcgagcac
     2161 gccaacagtt cgcactgccg cgaacgcgag ccccacgccg agcgccagtc cccaggctac
     2221 gagcacgagc gactcggcga ccgcgtcgcg cgtcgaatcc cacgccgagg cgccgagcgc
     2281 gacgcgaacg gccacctctc ggcgacggtg gacgccgcgc gccaacaaca gcgcgctcac
     2341 gttggtcacc gcgatgccta acaccagcgc caccgcgccc agcatcaacg ccagcggtgc
     2401 tctgagcggc gcgacgttct cggcctgcag cggcgccacg cgaacgctcg agccgcgcag
     2461 ctcgggatag gcgcgcgcga ggttggtcca gatgccgcgc agctccaact gggcgcttcg
     2521 cactgtagcg tgcggcgcca aacgtccaaa cgtttctagc ggatagttgt cgcgcacggc
     2581 ggcagcttgc ggagtgaaac ggagcggccg ccacacgtcg gccagaatca tccgcgagcc
     2641 gtcgggaacg cgaaaatcgg ccggcaagac gccaacgatt tgcgtcgcct ggccgttgag
     2701 caacacgctg cggccaacga ctgagggatc gctgccaaac gcgcggtg
//
LOCUS       DASRVO010000394         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_271621, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000394 DASRVO010000000
VERSION     DASRVO010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271621"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..287)
                     /locus_tag="VFF05_04925"
     tRNA            complement(198..287)
                     /locus_tag="VFF05_04925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Ser,seq:tga)
     gene            397..831
                     /locus_tag="VFF05_04930"
     CDS             397..831
                     /locus_tag="VFF05_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51180.1"
                     /translation="MTGRAVPLLLCIALSGCGGGSSEPREGTFTVTGGETRTTTNDKF
                     QLTGSAFLPMGAGCTYYPGPFAPPSCTCEPGQAASGQWTNSTTGAGGQLQLTIFADAT
                     CVARNAGWFTPAIALAPGSNSISVSMTDGVSRGAATVTVVRN"
     gene            complement(892..>1505)
                     /locus_tag="VFF05_04935"
     CDS             complement(892..>1505)
                     /locus_tag="VFF05_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZW51181.1"
                     /translation="MTSILSRVSPSITNMIRLDHTHVLSTFHQYEVDTSPRMKKGLVG
                     TVCVALDIHAQLEEEIFYPALRAVAETEVVQKSISEHQHMRELISSLRNMEPGDNEYD
                     KKFFELMNNVMHHVADEETLLLPAAERLLTGRLGELGAQMTKRRMQLAVPRTPEIARG
                     MARSMSTGTLLVAAGTLLAGGYLMTRPAENRWHWRRRTAGPIR"
BASE COUNT          243 a          491 c          505 g          266 t
ORIGIN      
        1 ggatgccgtg agccgccacg cgggttcggc gccggagaag ctcgccgcct gggaggcgcg
       61 caatcccgtc gcgttcgcgc gcgccaaacg gctggtggcc gaactggcgg atgcggccag
      121 cccggaactg gcgatgctgt cggtcgcgct gcgggagctg cgcaacctgg tctgaagtgg
      181 ggccggacac gctcccatgg cggagagtgc gagattcgaa ctcgcggacg ccttgcagcg
      241 tcgccggttt tcaagaccgg tgcattcaac cgctctgcca actctccagc agggcgattt
      301 tacgcggcgg cgctcacaag ttgatctgcc gcaaacttcc ccggattgcg cggcacacaa
      361 tcaatggcgt cgcttcgcag tacccgtagt tccgacatga caggccgcgc agttccattg
      421 cttctgtgca ttgccctgtc cggctgcggc ggcggatcgt cggagccacg ggaagggacc
      481 ttcacggtga ccggtggcga gacgcggacc accacgaacg acaaattcca gctcaccggt
      541 tccgccttcc ttccgatggg cgccggctgc acgtactatc cgggtccgtt cgcgccgccg
      601 agttgcacct gcgaaccggg ccaagcggcc agcgggcaat ggaccaattc gaccacgggc
      661 gccggcggac aactgcagtt gacgatcttc gccgacgcaa catgcgtcgc acggaatgcg
      721 ggatggttca cgcctgcgat cgcgctggcg cccggcagca attcgatttc ggtttccatg
      781 accgacggtg tgagccgcgg cgccgcgacg gtcacggtgg tgcgcaactg agcgcgcgcc
      841 gccgcgccgc catcgccggc cgggcagttg cggaaaacgc agagggcgcg atcagcggat
      901 cggccccgcc gtccggcggc gccaatgcca gcggttttcc gcgggccggg tcatcagata
      961 gccgccggcc agcagcgtac ccgccgcgac caacaaggtg ccggtgctca tcgagcgggc
     1021 catgccgcgc gcgatttcag gcgtgcgcgg cacggccagt tgcatgcggc gcttggtcat
     1081 ttgcgcgccg agctcgccca gccggccggt cagcaggcgc tcggcggcgg gcagcaacag
     1141 cgtttcctcg tcggcgacgt ggtgcatcac gttgttcatc agctcgaaga acttcttgtc
     1201 gtattcgttg tcgcccggct ccatgttgcg caggctcgag atgagttcgc gcatgtgctg
     1261 gtgctcggaa atgctctttt gcaccacctc ggtttcggcg accgcgcgca atgcgggata
     1321 gaagatttct tcctccagct gcgcgtggat gtccagcgcg acgcaaaccg tgccgaccag
     1381 ccctttcttc atccgcggcg aagtatcgac ctcgtactga tggaaggtgg acagcacatg
     1441 cgtgtggtcc aggcggatca tgttggtgat gctcggcgac actcggctca ggatggacgt
     1501 catgg
//
LOCUS       DASRVO010000395         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_323564, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000395 DASRVO010000000
VERSION     DASRVO010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323564"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFF05_04940"
     CDS             complement(<1..227)
                     /locus_tag="VFF05_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZW51182.1"
                     /translation="MSKLLVRSFAVSLDGFGAGANQSLENPLGERGPELMEWFFHSQF
                     WNRMHGKDGGETGVDNSIAEQGFDNLGAWILG"
     gene            complement(249..701)
                     /locus_tag="VFF05_04945"
     CDS             complement(249..701)
                     /locus_tag="VFF05_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZW51183.1"
                     /translation="MAYTHIVLAIRAGLGGLAAMYIPPGFNTITPYFFVKDANRFVAF
                     LIEGLGGVEVVRSMRPDGRVGNAQVRIGTSTIMASEAGGSYPPMPMACYLYVENADES
                     MARALAAGARLEMEVGDMPYGDRQGGVRDAHGNLWWISQRMVQEPYSA"
     gene            712..1401
                     /locus_tag="VFF05_04950"
     CDS             712..1401
                     /locus_tag="VFF05_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HZW51184.1"
                     /translation="MNYWPLLGVAVVVAGFALRMNPALVVVVAAIVSGMLSGLSVPDL
                     LALLGAKFIDSRNLLILVLTLPTIGLLERAGLREHAANWIARMRRFTFARLLIGYLAV
                     RQILSMFGLTGIAGHAQTVRPLLAPMAEAAAEKIDADIDLDERERIRAFAAATDNTGL
                     FFGEDIFIAFGAVLLIQGFYAQNGIVLDPRSIAIWALPTAIAAFVVQSVRAIRMQRRL
                     RAKTRREQAAP"
     gene            1398..2372
                     /locus_tag="VFF05_04955"
     CDS             1398..2372
                     /locus_tag="VFF05_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HZW51185.1"
                     /translation="MILRVEYVYWLCGALLAACALRELRARRWAHAAFWGILAAIFLL
                     GDAIKSAAAHGNAMPEQLVGAGVIALGLIAGFLRRAPVAESAPEHERRSASAARLGHR
                     LFMPALIIPFGTLVFTLLQPFVRVGGIDRLGRADATLLALGLACVLAGFAALALTRAR
                     ARDAIAETGRLLDALSWAALLPMLLAMLGAVFAVSGVGEAIAHLTAAAIPVQSRLACV
                     LAFAAGMVAFTVIMGNAFAAFPVMMAGVGLPLLIGQHHAQAAALGALGMLTGYCGTLL
                     TPMAANFNIVPAALLELRDPNGVIRAQWPTALWLLAINLALIWFFAFA"
     gene            2369..>2488
                     /locus_tag="VFF05_04960"
     CDS             2369..>2488
                     /locus_tag="VFF05_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HZW51186.1"
                     /translation="MKRRTPVVLVTGFDPFGGEAVNPSQLIVQALDGEIIAGHR"
BASE COUNT          367 a          855 c          793 g          473 t
ORIGIN      
        1 ccgaggatcc acgcgccgag gttgtcgaag ccctgttcgg cgatggaatt gtcgacgccg
       61 gtttcgccgc cgtccttgcc gtgcatgcgg ttccagaatt gcgagtggaa gaaccactcc
      121 atcaattccg gcccgcgttc gcccagcgga ttttccagac tttgattcgc cccggcgccg
      181 aagccgtcga gggacaccgc gaaactgcgc acgagcaatt tggacatgac gcgcctcatt
      241 cgtattcctt acgcggagta cggttcctgc accatccgct gcgagatcca ccagaggttt
      301 ccgtgcgcat cccgcacacc accctgtcga tcgccgtacg gcatgtcgcc gacttccatt
      361 tcgagccggg cgccggcagc gagcgccctg gccatggact cgtcggcatt ctcgacgtag
      421 aggtaacacg ccatcggcat tggcgggtaa gaaccgccgg cttcgctggc catgatcgtc
      481 gaggtgccga tgcgaacttg cgcattgccg acgcggccat ccggacgcat cgagcggacc
      541 acttcgacgc cgccgagtcc ttcgatcagg aacgcgacga accgattcgc gtccttgacg
      601 aaaaagtagg gcgtgatcgt gttgaatccg ggcggaatgt acatggcggc aagtcctccc
      661 aagccggcac ggatggccag cacaatatga gtgtatgcca tactcgtgcg catgaactac
      721 tggcccctgc tcggcgtcgc cgtggtcgtc gccggattcg cgctgcggat gaatccggcg
      781 ctggtggtcg tcgtcgctgc catcgtttcc gggatgctgt ccggcctgtc ggttcccgat
      841 ctgctcgccc tgctcggtgc gaagttcatc gacagccgca acctgctgat cctcgtgctg
      901 acgctgccga ccatcggcct gctcgaacgc gccggcctgc gcgagcatgc cgcgaactgg
      961 atcgcgcgga tgcgccgctt caccttcgcg cgcctgctga tcggctacct cgcggtacgc
     1021 cagattcttt ccatgttcgg acttaccggc atcgccggac acgcacaaac cgtgcgcccg
     1081 ctgctcgcgc cgatggccga ggccgcggcg gagaaaatcg acgcggacat cgacctcgac
     1141 gagcgcgaac gcatccgcgc cttcgcggca gcgacggaca acacgggtct gtttttcggc
     1201 gaggacatct ttattgcttt tggtgcggtg ctgctgatcc agggcttcta cgcgcagaac
     1261 ggcatcgtgc tcgatccgcg cagcatcgcg atctgggcgt tgccgacggc gatcgccgcg
     1321 ttcgtggtgc agagcgtacg cgcgatccgg atgcagcggc gccttcgcgc caaaacacgg
     1381 cgcgaacagg cagcgccgtg atcttgcgcg tcgaatacgt ctactggctg tgcggcgcat
     1441 tgctcgccgc ctgtgcgctg cgcgaactgc gcgcgcggcg ctgggcgcac gccgcgttct
     1501 ggggaatcct cgccgcaatt ttcctgctcg gcgacgcgat caaatccgcc gccgcacacg
     1561 gcaatgccat gcccgaacaa ctcgtcggcg cgggcgtgat cgcgctggga ctgatcgcag
     1621 gattcctgcg gcgtgcgccg gtcgcggagt cggcgcctga gcacgaacgt cgcagcgcca
     1681 gcgctgcgcg gctcggacac cgcttgttca tgccggcgct gatcattccg tttggcacgc
     1741 tggttttcac gctgctgcag ccatttgtgc gtgtcggcgg aatcgaccga ctcggacgcg
     1801 ccgacgcgac cctgctcgca ctcggactcg cctgcgtact cgccggattc gccgcgctcg
     1861 cgctcacgcg cgcgcgcgcg cgcgacgcca tcgccgaaac cgggcgcctg ctcgatgcgt
     1921 tgtcgtgggc ggcgttgttg ccgatgctgc tggccatgct cggcgcggtg ttcgccgtca
     1981 gcggcgtggg cgaggcgatc gcacacctca ccgccgccgc gattcccgtg cagtcgcgct
     2041 tggcctgcgt gctcgcgttc gccgccggca tggtcgcgtt caccgtcatc atgggcaacg
     2101 ccttcgcggc gtttccggtg atgatggccg gcgtcggcct gccgctgctg atcggccagc
     2161 atcacgcgca ggccgccgcg ctcggcgcgc tgggcatgct caccggctac tgcggcaccc
     2221 tgctcacgcc gatggcggcg aatttcaaca tcgtgccggc cgctctactc gaactgcgcg
     2281 atcccaatgg cgtgatccgc gcgcagtggc ccaccgcgct gtggttgctg gcgatcaatc
     2341 tcgcactcat ctggtttttc gcattcgcat gaagcgccgc acgcccgttg ttctcgtcac
     2401 cggcttcgac ccgtttggcg gcgaggcggt gaacccctcg cagctgatcg tgcaggcgct
     2461 ggatggcgag atcatcgccg gccatcgc
//
LOCUS       DASRVO010000396         5035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_141126, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000396 DASRVO010000000
VERSION     DASRVO010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141126"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1094)
                     /locus_tag="VFF05_04965"
     CDS             complement(96..1094)
                     /locus_tag="VFF05_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZW51187.1"
                     /translation="MKQISPGLRRLAWAAAAFALLVIVFGAFVRLSNAGLSCPDWPTC
                     YGKLSWPTQVHEIAHADAAFPQRPFEADKAWREQVHRMLVGVLSLLTFALAGWAWWRR
                     RELGRLVLVPCAAAGFIFFQAALGMWTVTWKLLPLVVTAHLVGGMAMFALLAYTALRL
                     TLHEPAPADSAKLRRMAIVGIVLVAIQIALGGWTSSNYAALACGTDFPTCLGQWWPAM
                     DFHQAFIVLRHIGVDYEGGILDGPARTAIQLVHRIGALVVFAHVLPLAIRAMRSRVLR
                     NYGFAIGGFLIAQVCLGISNVVFGLPLAVAAAHNACAALLLLSLLALAVRLRPARH"
     gene            complement(1096..1644)
                     /locus_tag="VFF05_04970"
     CDS             complement(1096..1644)
                     /locus_tag="VFF05_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51188.1"
                     /translation="MNKSTRGRLALLGIVIVFAVPLAAAFLLRGSGWQPARTLQSGIL
                     MQPPRDVSAAPVTLADGSRYAWQDSRYRWTLLMLPGSACAQNCQTRLDEVLRMRITLG
                     RNADRLRVLYLGPALAPDFLAVRAPLAAGRDDSGAFAFAHAQGADDLALALVDPRGQL
                     MLRYADGYSAQGLRSDVVKILY"
     gene            complement(1641..2381)
                     /locus_tag="VFF05_04975"
     CDS             complement(1641..2381)
                     /locus_tag="VFF05_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZW51189.1"
                     /translation="MTARHWQRPTTFAWILTAFGVAAFCALGLWQVDRAAQKRQLFAA
                     FANAAGSPTLDFAQATAAARDAQRYPHLRVTGHYLPDRGYWLDEQFSGNRIGVHAIGA
                     FAIDGQPDLLLVDRGWVAWEHLPKTAPIAPALPAGEMALTGLYAPFPGGGLRLGGDAL
                     PGQATWPKLTLFLDAKAIAVDLGVAVQPRMLLLDPEPGSVFVRVWKPQVFPPERHIGY
                     AFTWFCFAAVAVAMLVVKHWTKVEKRST"
     gene            2430..2639
                     /locus_tag="VFF05_04980"
     CDS             2430..2639
                     /locus_tag="VFF05_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HZW51190.1"
                     /translation="MWTTLIVIAFFVVILWNLGAGLYYMMTDKGTTDRTVRALTWRIG
                     LSVLLILLVILGILTGVIHPHNVGR"
     gene            complement(2645..3514)
                     /locus_tag="VFF05_04985"
     CDS             complement(2645..3514)
                     /locus_tag="VFF05_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HZW51191.1"
                     /translation="MSSEAPGAYYVPHGTHWPILGSFGLFFTVGGAALWMNEFEPGKY
                     VMVLGIACILSMMFGWFRTVIGESLSGIYNKQVDTSFRMGMMWFIFSEVMFFCAFFGA
                     LFYARTFSVPWLAGEGDGALTNFFLWKDFAAAWPTNGPAQIGGLFQTMPAWGVPLLNT
                     LILLTSGVTITIAHHALRAGNRRQLLIWLGATVVLGALFLNFQAHEYIEAYTKLNLTL
                     GSGVYGSTFFMLTGFHGLHVTLGAIMLSIIWLRCLKGHFTKNHHFAFEAVAWYWHFVD
                     VVWLGLFIFVYVL"
     gene            complement(3531..4124)
                     /locus_tag="VFF05_04990"
     CDS             complement(3531..4124)
                     /locus_tag="VFF05_04990"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HZW51192.1"
                     /translation="MAVDTATGDTVQRNRRFVMRALVMCGAMFLFGFACVPMYRIVCE
                     HVLKIRIYNTADSGKGFTVDESRWVTVQFVGNVNSQLDWQFTPERVEMKVHPGAINDA
                     WFDATNKAQSAIVGNAVPSIAPSQGSPYFNKIECFCFTEQTLKPGESRRMPVKFVVDP
                     KLPKSIDTLTLSYTFYNNRAATRKLVEQAQSATPSNS"
     gene            complement(4124..4255)
                     /locus_tag="VFF05_04995"
     CDS             complement(4124..4255)
                     /locus_tag="VFF05_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51193.1"
                     /translation="MKQTPAKSDIDQRRAAARRTAWIVAIVAVAIYLLFFLSQGLSH"
     gene            complement(4318..>5035)
                     /locus_tag="VFF05_05000"
     CDS             complement(4318..>5035)
                     /locus_tag="VFF05_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZW51194.1"
                     /translation="VWGHHMFTVGMPLGGELYFMYATMLIAIPTGVKVFNWVSTMWGG
                     SLTFETPMLWAIAFVILFTIGGFSGVMLALVPADFQYQDTYFVVAHFHYVLVTGALFS
                     IIAAIYYWWPKWTGKMYNETWSKFHFWWSMVFVNVLFFPQHFLGLAGMPRRIPDYNVA
                     FADWNLVSTIGAYGMFLTPLMMFFILWHSKKYGKPAAARSWEGAHGLEWTVPSPAPFH
                     TFAIPPVIHDEDLAHGDTSH"
BASE COUNT         1025 a         1574 c         1642 g          794 t
ORIGIN      
        1 aacatgccga tcaccgcaca gaacaccagc agcgcgacga tgcgcggctt ggtcagttcc
       61 aggtactggc gggcgatagc ggacatgcgc gcagtttaat gcctggccgg gcgcagccga
      121 accgccagcg cgagcaggga aagaagcaga agcgcggcgc aggcattgtg cgcggccgcc
      181 acggccagcg gcaatccgaa cacaacgttg ctgatgccaa ggcacacctg cgcgatcaga
      241 aatccgccga tggcgaatcc gtaatttcgc agcacgcgcg agcgcatggc gcggatcgca
      301 agcggcagca cgtgcgcgaa cacgaccagc gcgccgatgc gatgcacgag ctggatcgcg
      361 gtgcgcgcgg gaccatcgag gatgccgccc tcgtagtcca cgccgatgtg gcgcagcacg
      421 atgaatgcct ggtggaaatc catcgccggc caccactgac cgagacaggt cgggaaatcc
      481 gtaccgcagg cgagcgcggc gtaattgctg ctggtccagc cgccgagcgc gatctggatg
      541 gcgacgagga cgatgccgac gatggccatg cgccgcagtt tcgcactatc cgccggcgcc
      601 ggttcgtgca gggtcaggcg cagcgcggtg taggctagca gcgcgaacat cgccatgccg
      661 ccgacgaggt gcgcggtcac gaccagcggc agcaacttcc aggtcaccgt ccacatgccg
      721 agcgcggcct ggaagaaaat gaaaccggcg gcagcgcagg gaaccaacac gagacgtccg
      781 agctcgcgac ggcgccacca cgcccacccc gccagcgcga acgtgagcag gctcagcacg
      841 ccgaccagca tgcggtgcac ctgctcgcgc caggccttgt cggcctcgaa gggtctttgt
      901 gggaatgccg catccgcatg cgcgatctcg tgcacctgcg tcggccagct caacttgccg
      961 tagcaggtcg gccaatccgg acacgacagt cccgcgttgg acaggcgcac gaaggcgccg
     1021 aacacgatga cgagcagggc aaaggccgcc gcggcccagg ccagtcgacg caatccgggc
     1081 gaaatctgtt tcatttcagt agaggatttt cacgacgtcg ctgcgcagac cctgcgcaga
     1141 ataaccatct gcatagcgca gcatcagttg cccgcgcgga tcgaccaggg ccaaggccag
     1201 gtcgtcggcg ccttgcgcat gcgcgaacgc aaacgcgccg ctgtcgtcgc gcccggcggc
     1261 aagcggcgca cgcacggcga gaaaatccgg cgccagagca gggccgaggt aaagcacgcg
     1321 caggcgatcc gcgttgcggc cgagcgtgat gcgcatgcgc aggacttcgt cgaggcgcgt
     1381 ctggcagttc tgcgcgcacg cgctcccggg caacatcagc agggtccagc ggtagcgcga
     1441 gtcctgccac gcatagcggc tgccatcggc cagcgtcacc ggcgcggcgg aaacgtcgcg
     1501 cggcggctgc atcaggattc cgctttgcag ggtgcgcgcc ggctgccagc cgctgccgcg
     1561 cagcaggaac gcggcggcga gcggaactgc gaagacgatg acgatgccga ggagggccag
     1621 acggccgcgc gtggatttgt tcatgtcgat cgcttttcca cttttgtcca atgtttgacg
     1681 acgagcatgg cgacggccac cgcggcgaag caaaaccagg tgaacgcgta accgatgtgg
     1741 cgctcgggcg gaaatacctg cggcttccag acgcgcacga atacgctgcc cggttccggg
     1801 tcgagcagca gcatccgcgg ctgcacggca acgccaagat ccacggcgat ggccttggcg
     1861 tcgagaaaca aggtcagctt cggccaagtc gcctggcccg gcaatgcgtc gccgccgagc
     1921 cgcaatccgc cgcccggaaa cggcgcatac aaaccggtca gagccatctc tccggcaggc
     1981 aacgcgggcg cgatcggcgc ggtcttgggc aggtgttccc acgccaccca gccgcgatcc
     2041 accaggagca aatccggttg tccgtcgatc gcgaacgcac cgatcgcgtg cacgccgatg
     2101 cgattgccgc tgaattgttc gtccagccag taaccgcgat cgggcaggta atgtccggtt
     2161 acccgcagat gcggatagcg ttgcgcgtcc cgtgccgccg ctgttgcctg cgcgaaatcc
     2221 agcgtgggcg aacccgcggc gttcgcgaat gcggcaaaca gctgtcgctt ctgcgccgcg
     2281 cggtcgacct gccacaggcc caacgcgcaa aacgcggcga cgccgaacgc ggtcaggatc
     2341 catgcgaacg tggtcggacg ctgccaatgg cgcgcggtca tcgcgccgcg gcgttatact
     2401 cgccgcgctc cgccacggaa gatgcgccca tgtggaccac gttgatcgtc atcgcattct
     2461 ttgtcgtgat tctctggaat ctgggcgccg ggctttatta catgatgacc gacaagggca
     2521 ccaccgaccg cacggtacgc gcgctcacct ggcgcatcgg cctctcggtg ctgctgatcc
     2581 tgttggtgat actcggcatc ctgaccggcg tcatacatcc gcacaacgtc ggccgctagg
     2641 cgctctacaa tacgtagacg aagataaaca ggcccagcca caccacgtcg acgaagtgcc
     2701 aataccaggc cactgcctcg aacgcgaagt gatggttctt ggtgaagtga cccttcaggc
     2761 agcgcagcca gatgatcgac agcatgatcg cgcccagggt cacgtgcaga ccgtggaagc
     2821 cggtgagcat gaagaacgtc gagccgtaca cgcccgagcc cagggtgagg ttgagcttgg
     2881 tgtaggcctc gatgtactcg tgcgcctgga aattcaggaa cagcgcgccg agcacgacgg
     2941 tcgcgccgag ccagatcaac agttgccggc ggttgccggc gcgcagcgcg tgatgcgcaa
     3001 tggtgatggt cacgccggag gtgagcagga tcagcgtgtt gagcagcggc acgccccatg
     3061 ccggcatggt ctggaacagg ccaccgatct gcgcgggacc gttggtcggc caagcggcgg
     3121 cgaaatcctt ccacaggaaa aagttggtga gcgcaccatc gccttcgccg gccagccacg
     3181 gcaccgagaa cgtgcgcgcg taaaacagtg cgccgaagaa cgcgcaaaag aacatcacct
     3241 cggagaagat gaaccacatc atgcccatgc ggaacgaagt gtcgacctgc ttgttgtaga
     3301 tgccggacaa cgattcgccg atcaccgtgc ggaaccagcc gaacatcatc gacaggatgc
     3361 aggcgatgcc cagcaccatg acgtatttgc cgggctcgaa ttcgttcatc cacaaggcgg
     3421 cgccgccgac cgtgaagaac agcccgaacg agccgaggat cggccagtgc gtgccgtgcg
     3481 gaacgtaata agcgcctggt gcttctgagg acatggtcgg attccgtcga tcaactattg
     3541 gatggtgttg cggattgcgc ttgttcgacc agtttcctgg tcgccgcacg gttgttgtag
     3601 aaagtgtacg acagggtcag cgtgtcgatg ctctttggca gtttcggatc gaccacgaat
     3661 ttcaccggca tgcgccgtga ttcgccgggc ttgagcgtct gctcggtgaa gcagaagcac
     3721 tcgatcttgt tgaagtaggg cgaaccctgc gacggcgcga tgctcggcac ggcgttgccg
     3781 acgatggcgg attgcgcctt gttcgtggcg tcgaaccagg catcgttgat ggcgccgggg
     3841 tgcactttca tttcgacccg ctcgggcgtg aactgccagt cgagctggct gttgacgttg
     3901 ccgacgaatt gcaccgtgac ccagcgcgat tcgtctaccg tgaagccctt gccgctgtcg
     3961 gcggtgttgt agatgcggat cttcaacaca tgctcgcaga cgatgcggta catcggcacg
     4021 caggcgaagc cgaacaggaa catcgcgccg cacatgacga gcgcgcgcat gacgaaacgc
     4081 cggttgcgct ggacggtatc gccggtggcg gtatcgactg ccatcagtgc gacaaccctt
     4141 gcgacaggaa gaacagcagg tagatcgcca ccgccacgat cgcgacgatc cacgccgtgc
     4201 gccgcgcggc ggcgcggcgc tggtcgatat cggatttggc gggcgtctgc ttcattttga
     4261 aagtgcgtcc aggaatggac gcttcctgat tttcgcgttc agggatgaac gcacaaatca
     4321 atgactggta tcgccgtggg ccaggtcttc gtcgtggatg accggcggta tggcgaaggt
     4381 gtggaacggc gccggcgacg gcacggtcca ctccaggcca tgcgcgcctt cccacgaacg
     4441 cgcggcggct ggtttaccgt acttcttcga atgccaaagg atgaagaaca tcatgagcgg
     4501 ggtcaggaac atgccgtagg cgccgatcgt ggacaccagg ttccagtcag cgaaggcgac
     4561 gttgtagtcg gggatgcgcc gcggcatgcc ggcaaggccg aggaaatgct gcgggaaaaa
     4621 cagcacgttg acgaacacca tgctccacca gaaatggaac ttggaccagg tctcgttgta
     4681 catcttgccg gtccacttcg gccaccagta atagatcgcg gcgatgatcg agaacagcgc
     4741 gccggtcacg agcacgtagt ggaagtgggc gacgacgaaa taggtgtcct ggtactggaa
     4801 gtcggccggc accagggcca gcatgacgcc ggagaaaccg ccgatcgtga acaggatgac
     4861 gaacgcgatc gcccacagca tcggcgtctc gaacgtgagc gacccgcccc acatggtcga
     4921 cacccagttg aacaccttca ccccggtcgg gatcgcgatc agcatcgtcg cgtacatgaa
     4981 gtacagctcg ccgccgagcg gcatgccgac ggtgaacatg tgatggcccc agacg
//
LOCUS       DASRVO010000397         1429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_11779, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000397 DASRVO010000000
VERSION     DASRVO010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11779"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VFF05_05005"
     CDS             <1..504
                     /locus_tag="VFF05_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HZW51195.1"
                     /translation="LLLTSHGTDDHLLYVAMDPLPLDSIAPEDLADVFAKAKIRYKAI
                     VISACYSGGFIDALKDDTTMIVTAARADRSSFGCGSDSDITDFGRAFFVDGLNRNVTF
                     TGAFAEANKLIDGWETRDHDEHSLPQLVTTPRIEAQLKLWRSGIIIGAPVPFVAPAGS
                     DALTAAR"
     gene            complement(501..>1429)
                     /locus_tag="VFF05_05010"
     CDS             complement(501..>1429)
                     /locus_tag="VFF05_05010"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZW51196.1"
                     /translation="LIAENPVTGDFWKTLDGEPPRIIAHRGASGARPEHTLEAFALAL
                     AQGADMLEPDLVASRDGVLFARHDLGLSLSTDVATHPRFAARAREIGGVRDWWIGDFE
                     AHEIGILRAVQPMPDRGDRYDGQFGIASFAELLDLVARANRDREMPVVIEAEIKQPDA
                     AVLAALTRELESRDLAGEHAPVWIECFDHEFLRRARERCGNPCIVLLEAEGDWRRGDP
                     RLRELARWARGVAPRKDILRDAAGRDIRWVAAAHELGLEVHAWTFRDDRDFAPFVTPV
                     DELDAAFALGADALFCDFPDTAIAARAAFVAR"
BASE COUNT          243 a          500 c          450 g          236 t
ORIGIN      
        1 ctgctgttga ccagtcacgg caccgatgac cacttgctgt acgtggccat ggatccgcta
       61 ccgctggatt cgatcgcgcc ggaagacctg gccgacgttt ttgccaaggc gaaaatccgt
      121 tacaaggcga tcgtgatttc ggcctgctat tccggcggct tcatcgacgc gctgaaagac
      181 gacaccacga tgatcgtcac cgccgcccgc gccgaccgca gttcattcgg ttgcggcagc
      241 gattccgaca tcaccgactt cggtcgtgcc tttttcgtcg acggccttaa ccgcaacgtc
      301 acgttcacgg gtgcgtttgc cgaggcaaac aaattgatcg atggctggga aacccgcgac
      361 cacgacgaac actcgctgcc gcaactcgtg acgacgccgc gcatcgaggc gcagctcaag
      421 ctctggcgca gcggcatcat catcggcgcg ccggtaccgt ttgtggcgcc ggctggcagc
      481 gatgcgctga ctgcagcccg ttagcgcgcc acgaaagccg cacgcgcggc gatcgccgta
      541 tccgggaaat cgcagaacag cgcatccgcg ccgagcgcga aggcggcatc gagttcatcg
      601 accggcgtga caaacggtgc gaaatcccga tcgtctcgaa acgtccaggc gtgcacctcc
      661 aggcccagct cgtgcgcggc cgcgacccag cgaatgtcgc gcccggctgc gtcgcgcaga
      721 atgtccttgc gcggcgccac gccgcgcgcc cagcgcgcca gttcgcgcaa gcgcggatcg
      781 ccgcgccgcc aatcaccttc ggcctccagc agcacgatgc acgggttgcc gcagcgctca
      841 cgcgcgcgcc gcaggaattc gtgatcgaaa cattcgatcc acaccggcgc gtgctctccg
      901 gcgagatcgc gcgactccag ctcgcgcgta agcgcggcca gtacggccgc gtcgggctgc
      961 ttgatttccg cctcgatcac gaccggcatt tcgcgatccc ggttcgcccg cgcgacgagg
     1021 tcgagtagct cggcgaagct cgctatgccg aattgcccat cgtagcggtc gccgcgatcc
     1081 ggcatcggct gcaccgcgcg caggatgccg atctcgtgcg cctcgaaatc gccgatccac
     1141 cagtcgcgca cgccgccgat ttcgcgcgcg cgtgctgcga atcgcggatg cgtcgccaca
     1201 tccgtgctga gcgacaggcc aagatcgtgg cgcgcgaaca gcacgccgtc gcggctggct
     1261 acgaggtccg gttcgagcat gtcggcgccc tgcgcgagcg ccagggcgaa ggcttcgagc
     1321 gtgtgctctg ggcgcgcacc actggcaccg cggtgcgcga tgatgcgcgg cggttcgccg
     1381 tcgagcgttt tccagaagtc gccggtcacg ggattctccg caatcaatt
//
LOCUS       DASRVO010000398         1210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_115590, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000398 DASRVO010000000
VERSION     DASRVO010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115590"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1179)
                     /locus_tag="VFF05_05015"
     CDS             complement(184..1179)
                     /locus_tag="VFF05_05015"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZW51197.1"
                     /translation="MAAGRTRTITYREAVREALREALLRDKRVFLMGEDVGRYGGSYA
                     CSKDLLAEFGEERVRDTPLCESGFVGAGIGAAIGGMRPIVEVMTVNFSMLALDQIVNN
                     AATVHHMSGGQVSVPLVIRMATGAGRQLAAQHSHSLECWYAHVPGVRVVAPATVADAR
                     GMLWTALCDPNPVIMFEHATLYNDDGPVPADGDMAVDIDHAAIRRPGRDASIITYGGS
                     LKKALAAADALAIEGINCEVIDLRSLRPLDIPTLVDSVKRTHRALIVDEGWRTGSLAG
                     EISALLAEQTFFDLDAPIARVCSAEVPIPYPRHLEIAATPQVENIVAAVRGLLRR"
BASE COUNT          221 a          406 c          369 g          214 t
ORIGIN      
        1 caatagtcac gcggatgtct tctagcacac cggtttcgaa cgactcaatt tcgattgcgc
       61 ccttctgcgt ctcgactgcc gcgatcgcct cgccgcgctc tacgtgatcg cccgggcgct
      121 ttatccattc gatcagcgta ccggcctcca tgtcggcacc gagcgagggc atgcgaaacg
      181 cactcatcgg cgcagcaggc cgcgcacggc cgcgacgata ttctcgacct gcggtgtcgc
      241 agcaatctcg agatggcgtg ggtagggaat cggcacctca gcactgcaaa cgcgcgcgat
      301 cggtgcatcg aggtcaaaga acgtctgctc ggcaagtagt gcggaaattt cgcccgccag
      361 gctccctgtg cgccagcctt cgtcgacgat cagcgcgcgg tgtgtgcgct tgaccgaatc
      421 gactaacgtc gggatgtcga gcggccgcag cgagcgcagg tcgatcacct cgcaattgat
      481 gccctcgatg gcgagcgcat cagctgcggc cagcgccttc ttcaggctac cgccgtacgt
      541 gatgatgctc gcatcgcggc ccgggcggcg gattgccgcg tggtcgatgt ccaccgccat
      601 atcgccatcg gccggcactg gtccatcgtc gttgtacaac gtcgcatgct caaacatgat
      661 caccgggttc gggtcgcaca gcgcggtcca gagcatgcca cgcgcatcgg caacggtcgc
      721 cggcgcgacg acacggacgc caggcacgtg cgcgtaccag cactcgaggc tgtgcgagtg
      781 ctgcgcagca agctggcgcc cagcaccggt ggccatacgg atcaccagcg gcaccgagac
      841 ctgccctcca gacatgtggt gaacagtcgc agcgttgttg acgatttgat cgagcgccag
      901 catgctgaag ttgactgtca tgacttcgac gatcggtcgc atgccaccga tcgctgcccc
      961 gatgcctgca ccgacgaagc cggattcgca cagcggcgta tcgcggacgc gttcctcgcc
     1021 aaactcggcg agcagatcct tcgaacaggc ataagatccg ccgtagcgac ctacgtcctc
     1081 gcccataagg aacacgcgct tgtcacgcag cagcgcttcg cgcaaggctt cgcgcacggc
     1141 ctcgcgatag gtgatagtgc gcgtcctgcc ggccgccatc cgttcccgca gcggcacggg
     1201 aagttcggtc
//
LOCUS       DASRVO010000399         1232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_166488, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000399 DASRVO010000000
VERSION     DASRVO010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166488"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VFF05_05020"
     CDS             complement(<1..1189)
                     /locus_tag="VFF05_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51198.1"
                     /translation="MFRSPVPLVVRTRDYGQHWQSISKGLPAEIAWAVREDPVRKGML
                     YAAAGHGVFISFDDGDHWQSLQLNLPVSQMRDLAVHGNDLLVATFGRALWVLDDVSAL
                     RQVGPQVAISAAYLYQPAAAIRARWDMNTDTPLPPETPAAANPPDGAILDYYLRAQPR
                     EIALEVRDAKGKVVRKFTTTAEPAPAQPANAPEYWYRPMPTLPHAPGLNRFVWDLRYP
                     HPAVLTYGYFGAHLDYFEYTLPDHAIPGETPRWQPQGPLVAPGNYELVLTVDGQTYRQ
                     RLEVKPDPRVHAAQADYDAQLALEQRLTRGMAGSYEAWNQAHAARTALDAQKSKLGAN
                     AAKAAGDAIAATEKQLDVFEDGTRGTPGFGPLNRDLGRYIEMAGTADGRPSQLLLAAA
                     SAQC"
BASE COUNT          194 a          421 c          397 g          220 t
ORIGIN      
        1 cgcattgcgc cgaggcagcg gccagcagca gctgcgacgg ccgtccgtcg gccgtgcccg
       61 ccatttcgat gtaccgcccg agatcgcgat tgagcggtcc gaatccgggc gtgccgcgcg
      121 tgccatcctc gaatacatcc agttgctttt ctgtcgcggc gatcgcatcg cccgccgcct
      181 tggccgcatt cgcgcccagc ttcgactttt gcgcgtccag cgccgtgcgc gccgcgtgcg
      241 cctggttcca cgcttcgtag ctgccggcca tgccgcgcgt cagtcgctgt tcgagcgcta
      301 gctgcgcatc gtaatccgcc tgcgccgcat ggacgcgtgg gtcgggcttc acctccagcc
      361 tctggcgata cgtttgccca tccacagtga gtacgagttc atagttgccg ggcgccacca
      421 gcggcccctg tggctgccag cgcggcgttt cgcccgggat ggcgtgatcg ggcagcgtgt
      481 actcgaagta atcgaggtgc gcgccgaagt agccataggt cagcactgcc ggatgcggat
      541 agcgcaggtc ccacacgaag cggttcaagc cgggcgcatg aggcagcgtc ggcatcggcc
      601 ggtaccagta ttcgggagcg ttcgccggct gcgcgggcgc gggttcggcc gtagtcgtga
      661 acttccgcac tactttgccc ttcgcgtcgc ggacctcgag cgcgatctcg cgcggttgcg
      721 ctctcaggta gtaatcgagg atagcgccgt cgggcggatt ggcggccgcc ggcgtttccg
      781 gcggtaacgg cgtgtcggtg ttcatgtccc aacgcgcgcg gatcgccgcc gccggctggt
      841 acaggtacgc cgccgagatc gcgacctgcg gaccaacctg ccgcagcgcc gaaacgtcgt
      901 cgagcaccca cagcgcgcgc ccaaaggtgg ccaccagcag atcgtttccg tggaccgcaa
      961 gatcgcgcat ctgcgacacc ggcaggttca gttgcagcga ctgccaatga tctccatcgt
     1021 cgaacgagat gaacacgcca tggccggcgg cggcgtaaag cattcccttg cgcaccggat
     1081 cttcgcgcac cgcccacgcg atttccgccg gcagtccctt ggaaatcgat tgccaatgct
     1141 ggccataatc gcgcgtgcgc accaccagcg gcaccggcga tcggaacatt tgaaacacca
     1201 cgtatgccgt tcccggatcg aagtgaccgg gt
//
LOCUS       DASRVO010000400         1051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_38400, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000400 DASRVO010000000
VERSION     DASRVO010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38400"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /gene="mraW"
                     /locus_tag="VFF05_05025"
     CDS             complement(<1..229)
                     /gene="mraW"
                     /locus_tag="VFF05_05025"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HZW51199.1"
                     /translation="MRPPSEPSHTASTAGVWGSAYHAPVMAAEVVELFRGCDHVLDGT
                     LGGGGHALALLESGARVTGIDRDPDAVAAARE"
     gene            complement(323..>1051)
                     /locus_tag="VFF05_05030"
     CDS             complement(323..>1051)
                     /locus_tag="VFF05_05030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51200.1"
                     /translation="GEEMVQTDTSLADTSFFVRQAALYALINQPQKAAETTARGVAKF
                     KSNAALWSLDAQTQRLAGQTQAAVDAANQAIKLDPKAEHVYLRKAQAEMDLKQTDSAI
                     ATLKQALANGEDKGTIGQFLLVLGNGAYQAASDTSNGHKPSIAEFQHAIAVLSAADSI
                     APTPQDKFLMGVSYFRVADLAVRQNQTEKKCDLGKTAQDALLNAQIYLQQGGSVDPKT
                     AGQLLSILPKYSPAVDAQVKKYCK"
BASE COUNT          155 a          365 c          329 g          202 t
ORIGIN      
        1 gctcgcgcgc cgccgccacc gcgtccggat cgcgatcgat cccggtcacg cgcgcgccgg
       61 actcgagcag tgcgagcgcg tgcccaccgc cgccgagcgt accatcgagc acgtgatcgc
      121 acccgcggaa cagctcgacc acttccgccg ccatcaccgg cgcatgatac gcgctgcccc
      181 agacgccggc ggtcgacgcc gtgtgcgatg gttcgctcgg gggacgcata ctagcggcga
      241 acgatgcgcg gcgtcaagcg tgcctcctaa cgattgcgcc cgccgatcac gggcgggcgc
      301 atcgtgcatg tcgcgacatc gactacttgc agtatttctt gacctgcgcg tcgacggccg
      361 gtgagtattt cggcaagatg ctcaggagct gacccgcagt cttcgggtcc accgacccgc
      421 cctgctgcag gtagatctgc gcgttgagca gcgcatcctg cgccgtcttc ccgagatcgc
      481 acttcttctc cgtctgattc tgtcgaacgg caagatcggc gacgcggaaa tacgacacgc
      541 ccatcaggaa cttgtcctgc ggggtcggcg cgatgctgtc ggccgcggac agaaccgcga
      601 tcgcgtgctg gaactcggct atggatggct tgtgcccgtt ggacgtgtcc gacgcggcct
      661 gatatgcgcc gttgcccaac acgagcagga actgaccaat cgtgcctttg tcttcgccgt
      721 tggcgagcgc ttgcttgagc gttgcgatcg cgctgtcggt ctgcttgagg tccatttccg
      781 cctgcgcctt gcgcaggtac acgtgctccg ccttcggatc gagcttgatc gcctgattgg
      841 ctgcgtccac tgccgcttgc gtctggcccg cgagccgctg cgtctgcgcg tcgagcgacc
      901 acagcgccgc gttgctcttg aacttcgcga cgccgcgcgc cgtcgtctcg gcggccttct
      961 gcggctggtt gatcaacgca tacagcgccg cctgacgaac gaaaaagctc gtgtccgcga
     1021 gcgacgtgtc cgtctggacc atctcctcgc c
//
LOCUS       DASRVO010000401         1235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_11867, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000401 DASRVO010000000
VERSION     DASRVO010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11867"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VFF05_05035"
     CDS             complement(<1..1114)
                     /locus_tag="VFF05_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZW51201.1"
                     /translation="MSDLDRPWLANYPPGVPAQVDTNQFRSLAQVLETACDRFAHRPA
                     FENMGRTLTYADIDRLSRRFAAYLRNEIKLERGDRVAIMLPNVLQYPVAIFGALRAGL
                     TVVNTNPLYTARELRHQLHDAGVSAIVVLDNFAATLAEVLHDTPVKHIITTGIGDLLG
                     APKSMLVNFALKHVKKMVPAYRIDHAVRFNAALARGAHAAPPRVDIGHDDIAFLQYTG
                     GTTGVAKGAMLTHGNLVANMQQASAWIDRDVREGEEVIVTAIPLYHIFALTANCLVFM
                     KFGGLNHLVTNPRDMPGFVKILRGIRMTAITGVNTLFNGLLNTPGFDKVDFSALHLTL
                     GGGTAVQRSVAERWKKTTGVTLVEAYGLTETSPAACM"
BASE COUNT          226 a          353 c          414 g          242 t
ORIGIN      
        1 tcatgcatgc ggccggtgaa gtctcggtca ggccataggc ctcgaccaga gtcacgccgg
       61 tagtcttctt ccagcgttca gcaacgctgc gttgaaccgc ggtgccgcca ccgagagtca
      121 ggtgcaaggc ggaaaaatcc actttgtcga atcccggcgt attgagcaat ccattgaaca
      181 gcgtgttgac gccggtgatc gcagtcatgc ggattccacg caggatcttg acgaagcccg
      241 gcatgtcgcg cgggttggtg acgaggtggt tcaatccgcc gaacttcatg aacacgaggc
      301 agttcgcggt cagcgcaaag atgtggtaca gcggaatcgc ggtgacgatg acttcttcgc
      361 cttcgcgcac gtcgcggtcg atccaggccg aggcctgctg catgttcgcg accagattgc
      421 cgtgcgtgag catcgcgccc ttggccacgc cggtggtgcc gccggtgtat tgcaggaagg
      481 cgatatcgtc gtggccgata tcgactcttg gcggcgccgc gtgcgcaccg cgcgccagcg
      541 cagcgttgaa acgcacggcg tgatcgatgc gataggccgg caccatcttc ttgacgtgtt
      601 tgagcgcgaa attgaccagc atggatttcg gtgcgccgag caggtcgccg atgccggtgg
      661 tgatgatgtg cttgaccgga gtgtcgtgca ggacttcggc cagcgtcgcg gcgaaattgt
      721 cgagcactac gatcgcgctc acgccggcgt cgtgcaactg atgacgcagt tcgcgcgccg
      781 tgtacaacgg attggtgttg acgacggtga gccccgcgcg cagcgcgccg aagatcgcca
      841 ccgggtattg caggacgttc ggcagcatga tcgcgacacg atcgccgcgt tcgagcttga
      901 tttcgttgcg cagatacgcg gcgaagcggc ggctgagccg gtcgatgtcc gcgtaggtca
      961 gcgtccggcc catgttctcg aaggccggcc gatgtgcgaa acggtcgcag gcggtttcca
     1021 gaacctgcgc aagcgagcgg aactggttcg tgtcgacctg tgccggcacg ccgggcgggt
     1081 aattggccag ccaaggtcta tcgagatcgc tcatgacgcg ccatttcccc gggtctgtgg
     1141 attcgtcgat ttcaacacag ggcgggaaac cgcggcaagc tgcattgcgg cgcaggcggt
     1201 gatggtgcag catgcaaaca tgaaatactt tgtgt
//
LOCUS       DASRVO010000402         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_245398, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000402 DASRVO010000000
VERSION     DASRVO010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245398"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFF05_05040"
     CDS             <1..528
                     /locus_tag="VFF05_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrdJb"
                     /protein_id="HZW51202.1"
                     /translation="FINSKNMDHFQWIVALTRIMSAVFRKGGDVTFIVEELKAVFDPR
                     GGYFKAGGVYMSSIVAELGAIVEQHLKMIGLLHDPELSPEQRKLIADKRAAYESRGRD
                     AKKKSEIGTAGEMGGAMVKSSPALDEPAGEDSAAESSPAFPPGATLCHKCNTSAVIIM
                     DGCATCLNCGYSKCG"
     gene            569..946
                     /locus_tag="VFF05_05045"
     CDS             569..946
                     /locus_tag="VFF05_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HZW51203.1"
                     /translation="MRLDVWLWAARFFKTRALAKRALENGKIRIHDATGKPSRLLHVG
                     DRLRIARGDEQFEIDVIELIAQRGPASVAQACYRETEASHTARMQAAEQRRLSGTGYT
                     RPPTKPDKRARRLIRALGDIDAL"
     gene            1019..2719
                     /locus_tag="VFF05_05050"
     CDS             1019..2719
                     /locus_tag="VFF05_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51204.1"
                     /translation="MTMTGKIWLLIGLLAALPVQAAIDIDLSYVDQDSAEFARFKAYV
                     DRAVAGNPDYGFSAADAAYLYKLTAQSQYATLAVDTVEAQVSAAETVIAGGGRPDISG
                     DSYLEVGPMLEDLALTYDWCADHTTANQRTRWATYAEQAVWNVWHADTAQWGGHAFPW
                     SGWGVDDPANNYHYSFLRATAYWGLASNSSTWLNLLATAKWPEQEAYTATIPGGGSQE
                     GTAYGLSHRDLFMLYRVWRDSTGTDIANTNAHLTDSIQWWIHATVPTLDRVAAIGDQA
                     RVSEPVIYDYHRHLMLEARNLTNDATARENASWWLHSISDQDMQSSFNYRHNLLAAGD
                     AGTPPAALTYYATGTGQLFSRTGWDTHAMWLNFSAGPYVQSHAHQDQGAFTLFANDWL
                     AVTENIWTHSGIQQGTDTNNVLRFENAGAIVSQHEGTTSTMTVTPGVNGAVHAVANLT
                     PAYDGDPAVTSWQRTIDFAGHKLTVSDTFALGSGTSAIFQVDTPVQPVISGKTAQAGD
                     LLIRVLTPANATLSALDWTTQSDADETYNSGWRLDVSGGTSGYVVELSVDKIFSNGFN
                     "
     gene            complement(2774..>3032)
                     /locus_tag="VFF05_05055"
     CDS             complement(2774..>3032)
                     /locus_tag="VFF05_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51205.1"
                     /translation="AHALSTDPIQRRLFWQSRLVMLGLARRVLRRFEELARAVQTGNG
                     IAAAASYVVAGMAEYRRRNETTMYPLYQKIGQRTVGRPKVA"
BASE COUNT          605 a         1003 c          928 g          496 t
ORIGIN      
        1 ttcatcaact cgaagaacat ggatcacttc cagtggatcg tggcgctcac gcgcatcatg
       61 agcgccgtgt tccgcaaggg cggcgacgtc accttcatcg tcgaggaact caaggctgtg
      121 ttcgatccgc gcggcggcta cttcaaggcc ggcggcgtat acatgtcgtc gatcgtggcc
      181 gagctcggcg ccatcgtcga gcagcacctg aagatgatcg gcctgctgca cgatccggaa
      241 ctgtcgcccg aacagcgcaa gctgatcgcc gacaagcgtg cggcctacga gtcgcgcggg
      301 cgcgacgcaa aaaaaaagtc tgaaataggc actgccggcg aaatgggcgg ggcgatggtc
      361 aaatcctcac ccgccttgga cgaaccggca ggcgaagatt ccgccgcgga atcctcgccc
      421 gccttcccgc ccggcgcgac gctatgccac aagtgcaaca ccagcgccgt catcatcatg
      481 gacggctgcg cgacgtgcct gaactgcggt tacagcaaat gcgggtgaca accttctgaa
      541 taactccgct gcgccgccgg caaccggcgt gcgcctcgac gtgtggttgt gggccgcgcg
      601 ctttttcaag acgcgcgcac tggccaagcg ggcgctcgaa aacggcaaga tccgtatcca
      661 cgatgcgaca ggcaaaccgt ccaggctgct gcatgtcggc gatcgcctgc gcatcgcgcg
      721 tggcgacgaa caatttgaaa tcgatgtgat cgagctgatt gcacaacgcg gtccagccag
      781 cgtggcgcaa gcgtgttacc gcgaaaccga agccagccac accgcgcgga tgcaggctgc
      841 cgaacagcgt cgactaagtg gcaccggcta tacacgccca ccgaccaaac ccgacaagcg
      901 cgcgcggcgt ctgatccggg cactgggcga tatcgacgcg ctgtagcgcc aagcgtgtcg
      961 caattcacgc ctgactggcg ttgactcgtc taggctacga acacttccgg agttcgagat
     1021 gaccatgacg ggtaaaatct ggctgctgat cggactgctg gcggcgctgc cggtccaggc
     1081 ggcgatcgat atcgacttgt cgtatgtcga tcaggactcg gccgaattcg cgcgcttcaa
     1141 ggcctatgtc gatcgcgccg tcgccggcaa tcccgattac ggattttccg ccgccgacgc
     1201 ggcctacctg tacaagctga ccgctcaatc gcagtacgcg acgctggcgg ttgatacggt
     1261 cgaagcgcaa gtgtccgccg cggaaaccgt gatcgccggc ggtgggcggc cggatatttc
     1321 cggcgactcg tacctggaag tcggcccaat gctcgaggat ctcgcgctca cctacgactg
     1381 gtgcgccgat cacacgaccg ccaatcagcg cacccgctgg gcgacctacg ccgagcaggc
     1441 ggtatggaac gtctggcacg cagacaccgc gcagtggggc gggcatgcat ttccgtggag
     1501 cggctggggc gtggacgatc cggccaacaa ctaccactac agcttcctgc gcgcgacggc
     1561 gtactggggt ctggcgtcga atagctccac ctggctgaac ctgctggcga ccgcgaaatg
     1621 gcccgaacag gaagcgtaca ccgcgaccat tcccggcggc ggcagccagg aaggtacggc
     1681 gtatggtctt tcgcaccgcg atttgttcat gctgtatcgc gtctggcgcg actcgaccgg
     1741 caccgacatc gccaacacga acgcgcacct gaccgacagc atccagtggt ggatccatgc
     1801 caccgtgccg acgctcgatc gcgtggccgc gatcggcgat caggcgcgcg tctccgaacc
     1861 ggtgatctac gactaccacc ggcacctgat gctcgaggcg cgcaacctca cgaacgatgc
     1921 gaccgcgcgc gaaaacgcct cgtggtggct gcattcgatt tccgatcagg acatgcaaag
     1981 cagcttcaat taccggcaca acctgctggc cgcaggcgat gcgggtacgc cgcccgcggc
     2041 gctgacctac tacgccacgg gaaccggcca actgttttca cgcaccggct gggacacgca
     2101 cgcgatgtgg ctgaattttt ccgccggtcc gtacgtgcaa agccacgcgc atcaggatca
     2161 gggtgcgttc accttgttcg ccaacgactg gctggcggtg accgagaaca tctggacgca
     2221 cagcggcatc cagcaaggca ccgacaccaa caacgtgctg cgcttcgaaa acgccggcgc
     2281 aatcgtgtcg cagcacgaag gcacgacgtc gacgatgacg gtgacgccag gcgtgaatgg
     2341 cgccgtgcac gccgtggcga acctcacacc cgcctacgac ggcgatcccg ccgtgacctc
     2401 atggcaacgc acgatcgatt tcgctggcca caagctcacc gtcagcgaca cgttcgcgct
     2461 cggcagcgga acctcggcca tcttccaagt cgatacgcca gtgcagcccg tgatcagcgg
     2521 caagaccgcg caggccggcg acctgctgat ccgcgtgctc acgccggcaa acgcgacgct
     2581 cagcgcgctc gactggacca cgcaaagcga tgcggacgaa acctacaatt cgggttggcg
     2641 cctggatgtg tccggcggca ccagcggcta tgtcgtggag ctgagcgtcg acaagatttt
     2701 cagcaacggc ttcaattgag cggccgacac gccgtgaacg acgcccggat cattccgctg
     2761 cctggccgaa gcgttacgcc accttgggcc tgccgaccgt ccgctgtcca atcttctgat
     2821 acagcggata catcgtggtt tcgttgcgcc tgcggtattc ggccatcccc gcgacgacgt
     2881 agctggcagc cgccgcgata ccattgccgg tttgcactgc gcgcgcgagt tcctcgaaac
     2941 ggcgcaacac acgccgcgcc aatccgagca tgaccagacg cgactgccag aacaatcggc
     3001 gctggatcgg atctgtcgag agcgcatgcg cg
//
LOCUS       DASRVO010000403         1223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_271826, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000403 DASRVO010000000
VERSION     DASRVO010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271826"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="VFF05_05060"
     CDS             complement(<1..1138)
                     /locus_tag="VFF05_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZW51206.1"
                     /translation="MKIQFLGAAGTVTGSKYLITQADNRVLVDCGLFQGLKNLRLRNW
                     AELPFEPASISAVVLTHAHLDHSGYLPLLVKRGYTGPVYCTPATAELCKILLPDSGHI
                     QEEDAEYANRGGFSKHHPALPLYTEEDAMRCLRQLRTVDFNEDFAIGPGLQGRFTPAG
                     HILGAACVTVTGTAGKLVFSGDLGRPHDGIMNPPAPLSDADYIVVESTYGDRTHPSVD
                     ALDELAQIILRTVNRGGCVIVPAFAVGRTQTLLHFIHQLKAQGRIPSQLPVFLNSPMA
                     TDVTTLYERFGSELRLPKSEVAAMCRAATFVNSVEQSKRLNTLRNPMIIVAASGMATG
                     GRVIHHLKAFAPDPRNTILFSGYQAAGTRGAAMLGGAKEIRIHGQ"
BASE COUNT          202 a          372 c          425 g          224 t
ORIGIN      
        1 cctgaccgtg gatgcggatt tccttggcgc cgccaagcat ggccgcgccg cgcgtgccgg
       61 ccgcctgata gcctgaaaac aggatcgtgt tgcgcgggtc cggagcgaac gctttcaagt
      121 gatggatcac gcgaccgccg gtggccatgc cgctcgcagc gacgatgatc atcggattgc
      181 gcagggtgtt caggcgtttg gattgttcca ccgagttgac gaacgtggcc gcgcggcaca
      241 tcgcggcaac ttcgcttttc ggcaggcgca attcgctgcc gaaacgttcg tacagcgtgg
      301 tcacgtcggt cgccatcgga ctgttcagga ataccggtag ctgcgatgga atccggccct
      361 gcgccttgag ttggtggatg aaatgcagca gggtttgcgt gcgcccgacc gcgaatgccg
      421 gcacgatgac gcagccgccg cgattgacgg tgcgcaggat gatttgcgcc agctcgtcga
      481 gcgcatccac gctcgggtgg gtgcggtcgc cgtaggtgga ttcgaccacg atgtagtcgg
      541 cgtcgctcag cggcgccggc ggattcatga tgccgtcgtg cgggcggccc agatcgccgg
      601 agaacacgag cttgccggcc gtacccgtca ccgtgacgca ggccgcgccg aggatgtgcc
      661 cggcgggcgt gaagcggcct tgcaggcccg ggccgatggc gaaatcctcg ttgaaatcca
      721 ccgtgcgcaa ctggcgcagg cagcgcatgg cgtcttcttc cgtgtacagc ggcaatgccg
      781 gatggtgctt ggagaagccg ccgcgattgg cgtattcggc atcttcttcc tggatgtgcc
      841 cgctgtcggg cagcaagatc ttgcacagtt ccgccgtcgc cggcgtgcag tacaccggtc
      901 ccgtatagcc gcgcttgacg agcaggggca ggtagccgct gtgatccagg tgcgcatggg
      961 tcagcacgac cgcggaaatc gacgccggct cgaacggcag ttcggcccag ttgcgcaggc
     1021 gcaggttctt caggccctgg aacaggccgc agtcgacgag aacgcgattg tccgcctgcg
     1081 tgatcaggta cttcgaaccg gtgaccgtgc cggcggcgcc gagaaactgg attttcatgt
     1141 cgggtggttc cgcaccgtgg ctcggaagta gtctaacggc ttcaccgcca cgcgcgcggc
     1201 cacgccggaa tggtgcaggc atg
//
LOCUS       DASRVO010000404         1224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_38475, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000404 DASRVO010000000
VERSION     DASRVO010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38475"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VFF05_05065"
     CDS             complement(<1..909)
                     /locus_tag="VFF05_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW51207.1"
                     /translation="MHRTDVANPAYFHKVVDCQWACPAHTPVPEYIRLIAQGRYTDAY
                     MVNWNSNVFPGVLGRTCDRPCEPACRRGRVEENNGEHPEPVAICRLKRVAADMKDDVA
                     ARMPAIRPKNGKRIACVGAGPASLTVARDLAPLGYDITVFDGEPKAGGFMRTQIPRFR
                     LPESVLDEETGYILNMGVHFRNNRAITSMKALMAERYDAIFVGCGAPRGRDLDIPGRK
                     EAARFIHIGIDWLASVSFGHIKTIGKRVIVLGGGNTAMDCCRSARRLGGADVKVIVRS
                     GFEEMKASPWEKEDAMHEGIPIINFQV"
BASE COUNT          205 a          390 c          408 g          221 t
ORIGIN      
        1 aacttggaag ttgatgatcg ggatgccttc gtgcatcgca tcttccttct cccagggcga
       61 agccttcatc tcctcgaacc cgctgcgcac gatcaccttg acgtcggcgc cgcccaggcg
      121 gcgcgcggaa cggcagcagt ccatcgccgt gttgccgccg cccagcacga tcacgcgctt
      181 gccgatcgtc ttgatgtggc cgaatgaaac cgacgccagc cagtcgatgc cgatgtggat
      241 gaaacgcgcc gcttccttgc ggcccggaat gtcgagatcg cgcccgcgcg gcgcgccgca
      301 gccgacgaag atcgcgtcgt agcgctccgc catcagcgcc ttcatcgacg tgatcgcccg
      361 gttgttgcga aagtgcacgc ccatgttgag gatgtagccg gtctcctcgt cgagcaccga
      421 ctcgggcagc cggaagcgcg ggatctgcgt gcgcatgaag ccgcccgcct tgggctcgcc
      481 gtcgaacacc gtgatgtcgt agccgagggg cgcgagatcg cgcgcgacgg taagcgatgc
      541 cggtccggcg cccacacagg cgatgcgctt gccgttcttc ggtcggatcg ccggcatgcg
      601 ggccgccacg tcgtccttca tgtcggcggc cacgcgcttg agccggcaga tcgcgaccgg
      661 ttccggatgt tcgccgttgt tctcctcgac gcggccgcgc cggcaggcag gctcgcaggg
      721 ccggtcgcag gtgcggccga gcacgcccgg aaagacgttg gagttccagt tgaccatgta
      781 ggcgtcggtg tagcgaccct gcgcgatcag gcggatgtat tcaggaaccg gtgtatgggc
      841 gggacaggcc cactggcaat cgacaacctt gtggaaatac gccggattgg cgacatctgt
      901 tcgatgcaaa ctgacctccc tggactggtc cacgccgccc cgggtcccgg agcgcgagca
      961 tgcgcggaga gtctacgcga cacgtgcaaa aaagtaacgc tccgggggaa tccggagcgt
     1021 gtgggcggaa ggtcagccgg gtcggtccgg attcggtacc tcggagccga acttccatga
     1081 atccggattt gacctgttcc ggcgctcgag cgcgttgaca ctcatcaact ggtcggatga
     1141 ggtgtcttgc tactggtgcg atgcgtgctg cgcaagtgca tcgaaagcgc ggatcagttc
     1201 cggtggcgcc tgcaccagtt cgat
//
LOCUS       DASRVO010000405         1346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_141279, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000405 DASRVO010000000
VERSION     DASRVO010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141279"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..>1346
                     /locus_tag="VFF05_05070"
     CDS             183..>1346
                     /locus_tag="VFF05_05070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW51208.1"
                     /translation="MPSTTDQASAKITFRRFKDGDGKWKRWQEQIFDADHTHKCPTYI
                     QATPPCQGSCPSGEDIRGYLNIVRGVEKPPAGQTWQEYAFQSLAGANPFPSVMGRVCP
                     APCESGCNRNEVEDFVGINSVEHFLGDNAIAKNFSYEKPTVTSGKTVAVIGGGPAGLS
                     CAYQFARKGHAVTVFDEHAELGGMMRYGIPGFRTPRNILDAEIQRILDLGVRTRLKCR
                     VGVDITMEQIRAEFDAVFLGLGAQSGRPLPCPGSEAPNIVTATAFLQAFNDGRMRHVG
                     KRVVVVGGGDTSIDVVTVARRLGHIVHAHDADQPERAIAGYSAHDVAEVSARQGAEVV
                     LTSVFAVDGMQANKHEVEQATAEGITIRGSLVPVAVVCGPDGRATALRVARCEA"
BASE COUNT          263 a          431 c          433 g          219 t
ORIGIN      
        1 cgaccatggg gcgctacgtc tacaccgact acgatcgctg catcggttgc catatctgta
       61 tggacgtgtg cccgaccggg tacatcaaga tggggctgga gatgtagatg tcgcagcgca
      121 cgcatcagcc ggcgaacgaa ccggcgtaaa tccaaataac acaacacaga ggagcagcaa
      181 cagtgccgag caccaccgat caagccagcg ccaaaatcac ttttcgccgt ttcaaggacg
      241 gcgacgggaa atggaagcgc tggcaggaac agattttcga tgcggaccat acgcacaagt
      301 gcccgaccta tattcaagcc acgcccccct gccagggcag ttgcccttcg ggcgaggaca
      361 tccgcggcta tctgaacatc gtgcgcggcg tggaaaaacc gcccgccggg caaacctggc
      421 aggaatatgc gtttcagagc ctcgccggcg ccaacccgtt cccttcggtg atgggacgcg
      481 tgtgcccggc gccgtgcgag tccggctgca accgcaacga ggtcgaggat ttcgtcggca
      541 tcaactcggt ggagcacttc ctcggcgaca acgcgatcgc caagaatttc agctacgaga
      601 aaccgacggt gaccagcggc aagaccgtgg cggtgatcgg cggcggaccg gcaggcctgt
      661 cctgcgccta tcagtttgcg cgcaaaggcc atgcggtcac cgttttcgat gaacatgcgg
      721 agctcggcgg gatgatgcgc tacggcattc ccggattccg cacgccgcgc aatatcctcg
      781 acgccgaaat ccaacgcatt ctcgacctcg gcgtgcggac ccgcctgaaa tgccgcgtcg
      841 gcgtcgatat cacgatggaa cagattcgcg ccgagttcga tgcggtgttc ctcggcttgg
      901 gtgcgcaatc ggggcggccg cttccctgcc caggcagcga ggcgccgaac atcgttaccg
      961 ccaccgcgtt tctgcaggcg ttcaacgatg gccgcatgcg ccacgtgggc aagcgcgttg
     1021 tcgtggtcgg cggcggcgat acctcgatcg acgtcgtcac ggtcgcgcgg cgcctgggcc
     1081 acatcgtgca tgcccacgat gcggaccaac ccgagcgtgc gatcgccggc tatagcgcac
     1141 atgatgtcgc cgaagtctcc gccaggcagg gcgccgaggt ggtgctcacc tcggtgtttg
     1201 cggtcgacgg gatgcaggcg aacaagcatg aggtcgagca ggccactgcc gaaggcatca
     1261 ccatccgcgg cagcctggta ccggtcgcag tggtgtgcgg ccccgacggc cgcgccaccg
     1321 cgctgcgcgt ggcgcggtgc gaagcg
//
LOCUS       DASRVO010000406         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_89232, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000406 DASRVO010000000
VERSION     DASRVO010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89232"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1548
                     /locus_tag="VFF05_05075"
     CDS             178..1548
                     /locus_tag="VFF05_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51209.1"
                     /translation="MTILALACAFSATAAPDGFSSDPLTTPDASGWLRTFTPAGTIDT
                     GNPFFQSLGTNGRSCNSCHRQAQGWTVTPAELQQRFAATQGLDPIFRTNDGSVSPFAD
                     VSTLAARRKAYALLLNRGLIRVGLPIPANAEFSLTAVDDPYHYASAAELSLFRRPLPA
                     TNLGFLTTVMWDGRETAAPFKPPMDAGVDSADLDASLASQAKDAVLGHAQGAAAPSDA
                     VLAQIVAFESGLSTAQIRDDNAGLLNDDDAIGGPRVLANLRFYVGNNDALGADPTGAA
                     FDPSAMHLFDAWATQHGNGRTERARAAIQRGEVLFNTFPITITGVAGLNDVTGQPSIQ
                     GHCSTCHDTPNVGNHSVVAPLDIGLADASRRTPDMPLYTLTNNQTGVSVQTTDPGRAL
                     LTGKWADIGKFKGAILRGLAARAPYFHNGSAATLMDAVDFYNTRFNLNLGAQQKQDLV
                     AFLAAL"
BASE COUNT          286 a          585 c          493 g          267 t
ORIGIN      
        1 aacggcttcg aatcgaccca gtgatccttc gcgccgcgga atagcggcgt cgacgatgtc
       61 gcaatccgtt ttcgtttggc gttactcgcc atcacagggg caatccgata gaagcgcgat
      121 acccgcgccg gggagatttg catgaaccgc ctgtgccgca attcggtacg tcccgtaatg
      181 acaattctgg cgcttgcctg tgcgttcagc gccacggcag cgccggatgg attctcgtcc
      241 gacccgctaa cgacgcccga cgccagcggc tggctgcgca cgttcacgcc ggcgggcacg
      301 atcgacaccg gcaatccttt cttccaaagc ctcggcacga acggccgcag ctgcaattcc
      361 tgccaccgtc aggcgcaagg ctggacggtc acgcccgcgg aactgcaaca gcgcttcgca
      421 gccacgcaag gcctggatcc gatcttccgc accaacgacg gctctgtttc gccgttcgcc
      481 gacgtgtcca ccctcgccgc gcgccgcaag gcctacgcgc tgttgctcaa tcgcggcctg
      541 atccgcgtcg gcctgccgat tccggccaat gccgagttca gcctgaccgc ggtggacgat
      601 ccctaccact acgccagcgc ggcggaactg tcgctgttcc gccgaccgct gccggcaacc
      661 aacctcggct ttctcaccac ggtaatgtgg gacgggcgcg aaaccgcggc accgttcaag
      721 ccgccgatgg atgcgggcgt ggacagtgcc gatctcgacg cgagccttgc ctcgcaggcg
      781 aaggatgcgg tactcggcca tgcgcagggc gcggccgcgc caagcgatgc cgtgctggcg
      841 cagatcgtcg ccttcgaatc aggactcagc accgcgcaga ttcgcgacga caacgccggc
      901 ttgctcaacg atgacgacgc gatcggcggg ccgcgcgtgc tggccaacct gcgcttctac
      961 gtcggcaaca acgatgcgct cggcgccgat ccgaccggcg cggccttcga tccgtccgcg
     1021 atgcacctgt tcgatgcatg ggcaacgcag cacggcaatg gccgcacgga acgcgcccgc
     1081 gcggcgatcc agcgcggcga ggtgctgttc aacacgtttc cgatcacgat taccggcgtc
     1141 gcgggtttga acgacgtcac cggccaaccg tcgatccagg gccactgcag cacctgccac
     1201 gacacgccga acgtcggcaa tcactcggtc gtcgcaccgc tcgacatcgg cctggccgat
     1261 gcttcgcggc gcacgccgga catgccgctc tacacgctga ccaacaacca gaccggcgtc
     1321 agcgtgcaga cgaccgatcc ggggcgcgcg ctgctcaccg gcaaatgggc cgacatcggc
     1381 aagttcaagg gcgcgatcct gcgcgggctc gccgcgcgag caccgtattt ccacaacggt
     1441 tcggcagcga cactgatgga cgccgtcgat ttctacaaca cgcgcttcaa cctcaacctc
     1501 ggcgcgcagc agaaacagga tctggtcgcg ttcctggcag ccctgtagca gccgtctcaa
     1561 cgccgcgctg cgtggcgtgc ccgcggttcg agcacgcctt tttacgtcac gttcggctcg
     1621 ctgaatgcct c
//
LOCUS       DASRVO010000407         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_245522, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000407 DASRVO010000000
VERSION     DASRVO010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245522"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VFF05_05080"
     CDS             <1..1243
                     /locus_tag="VFF05_05080"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HZW51210.1"
                     /translation="RAMTLTSARTFGWFAGLLICVMVFFAIGVAQVLGDDSFDLFYIG
                     FGEHTSRIGRGLAAALLGLVVYLIWQAFAVKRPQLTLPDRAELERARDLYNAHGGGEF
                     AHLTFMADKHLFWAADHQAVIAYGSVRDRLVALGSPCGADAAIARAILDFRHFADAQD
                     RVPVFYEVLEPDLSRYHDHGFDLFKLGELALVRLEEFSLAGKRWEDLRQAMNRAQKEK
                     LTFTLVQPPFDTALLTDLERVSDAWLADKGGDEKGFSLGRFDPAYFAWSPLGLVHRDG
                     ELIAFANVLPPYGPGGHASVDLMRHVADTPRSTMDFLFANVMQWAQQQGHELFSLGMA
                     PLSRVGDNPYARVNERLAALAFQYGNRFYNYQGVRKYKDKFKPEWVGSYLAYPRGLWV
                     PGLLIDIAALVSGGYRRLLVG"
     gene            complement(1244..>2268)
                     /locus_tag="VFF05_05085"
     CDS             complement(1244..>2268)
                     /locus_tag="VFF05_05085"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HZW51211.1"
                     /translation="VLVGDGAGAVFAYALDAQAPQGTYAGLVTLGWDSTFRLPKPVCK
                     GDAGPMTAPDGSGRFRIVPVKALPNPWLPQPFAPGARSTGLARHLPSALHEWFAALNG
                     TDTDPGKTLAAAIDWLTAPARAAPPLPRDIADLPLESVPAQGVFAQRIAIILTGDGGW
                     AGLDVAVANQLAQRGIEVVALNTLKYFWETRTPDQAVDALTGIIGHYGAQHPLADFVV
                     LGYSFGAGLSPVLINRLPESARKRIAAQVLISPDAEAVFEIHVGDWFGSTAHKGAIPI
                     APEIAKSQVPVICVHGADEDDSFCKRIVTKPNVRELVLPGAHHYNGDYDRLGAAIVGA
                     LPARKD"
BASE COUNT          363 a          765 c          737 g          403 t
ORIGIN      
        1 ccgtgcgatg acgctgacct cggcgcgcac ctttggctgg ttcgcgggcc ttctgatctg
       61 cgtaatggtg ttcttcgcga tcggcgtggc ccaggttctc ggcgacgatt ccttcgacct
      121 gttctacatc ggtttcggcg agcacacctc gcgcatcggc cgcggtctcg ccgccgccct
      181 gctcggactc gtcgtctatc tcatctggca ggccttcgcg gtcaagcgcc cgcagctcac
      241 cttgcccgac cgcgccgaac tggaacgcgc acgcgacctg tacaacgcac atggcggcgg
      301 cgagttcgcg cacctgacct tcatggccga caagcacctg ttctgggccg ccgaccacca
      361 ggccgtgatc gcctacggca gcgtgcgcga ccgcctcgtc gcgctcggct cgccctgcgg
      421 cgcggatgcc gcgatcgccc gcgcgatcct cgacttccgc cacttcgccg atgcacagga
      481 ccgcgtgccg gttttctatg aagtgctgga accggacctg tcgcgctatc acgaccacgg
      541 cttcgacctg ttcaagcttg gcgaactcgc gctggtacgg cttgaggaat tctccctcgc
      601 cggcaagcgt tgggaagacc tgcgccaggc catgaaccgc gcgcaaaagg aaaagttgac
      661 ttttacactt gtgcaaccgc cgttcgatac ggccttgctc acggaccttg aacgcgtttc
      721 cgatgcctgg ctcgccgaca agggtggcga tgaaaaggga ttctcgctcg gccgtttcga
      781 tccggcctac ttcgcctgga gtccacttgg cctcgtgcac cgcgacggcg aactgatcgc
      841 gttcgccaac gtgctgccgc cgtacggtcc gggcggccac gccagtgtcg atctcatgcg
      901 ccacgtcgcc gacacgccgc gcagcaccat ggacttcctg ttcgctaacg tcatgcaatg
      961 ggcgcagcag caaggccatg agttgttcag cctcggcatg gcgccgctgt cgcgcgtcgg
     1021 cgacaacccc tacgcgcgcg tcaacgagcg cctcgcggcg ctcgccttcc agtacggcaa
     1081 ccgcttctac aactaccagg gcgtgcgcaa gtacaaggac aagttcaagc ccgaatgggt
     1141 cggctcctac ctggcttatc cgcgcggcct gtgggtgccg ggcctgctca tcgacatcgc
     1201 cgcgctggtg tcgggcgggt atcggcggct gctggtcggc tagtcaatcc ttgcgcgccg
     1261 gcagcgcgcc gacgatcgcc gcgccgagtc tgtcgtaatc gccgttgtag tggtgcgcgc
     1321 cgggcagcac gagttcgcgc acgtttggct tggtgacgat tcgcttgcag aagctatcgt
     1381 cctcgtcggc accgtgcacg cagattaccg gcacctggga tttggcaatt tccggcgcga
     1441 tgggaatggc gcccttgtgc gcggtcgagc cgaaccagtc gccgacgtgg atttcgaaca
     1501 cggcttcggc gtcgggcgag atcagcacct gcgcggcgat gcgcttgcgc gcggattcgg
     1561 gcaggcggtt gatgagtacg ggcgacagac ccgcgccgaa ggaataaccg agtacgacga
     1621 aatccgcgag cggatgctgc gcaccatagt ggccgatgat gccggttaac gcgtcgacgg
     1681 cttgatccgg cgtgcgtgtc tcccagaaat acttgagcgt attgagtgcg acgacctcga
     1741 tgccgcgctg cgcaagctgg ttggcgacgg cgacatccag gccggcccag ccgccgtcgc
     1801 cggtcaggat gatggcgatg cgctgcgcaa acacgccctg cgccggcacg gattccagcg
     1861 gcaggtcggc gatgtcccgc ggcagcggcg gtgcggcacg cgcgggtgcg gtaagccagt
     1921 cgatggccgc agccagcgtc ttgccgggat cggtgtcggt gccgttcagc gcggcaaacc
     1981 attcgtgcaa ggccgacggc aggtgccggg ccaatcccgt actgcgcgca cccggagcga
     2041 acggctgcgg cagccacgga tttggcaggg ccttcaccgg cacgatgcga aaacgtccgc
     2101 tgccgtccgg cgcggtcatc ggtcccgcat cacccttgca caccggcttg ggcaggcgga
     2161 acgtcgaatc ccagccgagc gtgacgagtc cggcgtaggt gccttgcggc gcctgtgcgt
     2221 cgagcgcgta agcgaaaaca gcgcccgcac catcgccaac caagacgg
//
LOCUS       DASRVO010000408         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_245537, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000408 DASRVO010000000
VERSION     DASRVO010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245537"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFF05_05090"
     CDS             complement(<1..550)
                     /locus_tag="VFF05_05090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZW51212.1"
                     /translation="MNAAIDAMQAPEPAILEWDALDAGARAAALRRPALAQSSEIAPN
                     VARLLAQVRADGDAALRALTRRYDACDLVDLRVGDEEFAEAENALDPALKQAIAAAKG
                     RIEAFHRATAPQPTAVETAPGVRCERVLRPIASVGLYVPAGTAPLPSTALMLGVPARI
                     AGCREIAMCTPANATGHADPAVL"
     gene            complement(547..1443)
                     /gene="hisG"
                     /locus_tag="VFF05_05095"
     CDS             complement(547..1443)
                     /gene="hisG"
                     /locus_tag="VFF05_05095"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZW51213.1"
                     /translation="MSAAVDRERLRVAVQKSGRLADLSQDLLARCGLKFRQSRDKLFC
                     YGESMPIDLLLVRDDDIPGLIEQGVCDIGIVGRNVLAEHHSIGIGGDIVELRGLDRAR
                     CRLVIAVPQAMACATAQDLHGLRIATSYPGLLGAWLAQQGVDARIVELSGSVEIAPRL
                     GTADAVCDLVSSGATLMANQLSERFTLLDSEAVVAGARCAPADARGELLQLLLRRIDG
                     VLRVRESKLVMLRADRVSLERINALLPNPQMPTVTPVEGDDGRIALQAVCDGPVTWQH
                     LEDLRRAGASQLLVLPVEKLLA"
     gene            complement(1440..1784)
                     /locus_tag="VFF05_05100"
     CDS             complement(1440..1784)
                     /locus_tag="VFF05_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HZW51214.1"
                     /translation="MKRRQLDPEKSASSAEDDLCRALLSLRTPAQMLAFLRDLCTPAE
                     LEAMTDRWQVVPYLLQGMAYRDIHAHTAVSVTTIGRVARSLTQGNGGYRAAIAADALR
                     GRARQATSRPRP"
BASE COUNT          335 a          680 c          668 g          303 t
ORIGIN      
        1 ggagtacggc cgggtcggcg tgaccggtcg cattcgccgg cgtgcacatg gcgatttccc
       61 ggcagccggc gatccgtgcc ggcactccga gcatcaacgc ggtcgatggc aagggcgccg
      121 tacccgccgg cacgtacagg ccgaccgagg cgatcggccg cagcacacgc tcgcagcgca
      181 cgcccggtgc ggtttcgacc gccgtcggct gtggcgcagt ggcgcgatga aacgcctcga
      241 ttctcccctt cgccgcggcg atggcctgtt tcagagccgg atcgagtgcg ttttccgcct
      301 cggcgaattc ttcgtcgccg acgcgcaagt ccaccaggtc acaggcgtcg tagcggcgcg
      361 tcaacgcgcg cagcgcggca tcgccgtcag cgcgaacctg tgccagcagg cgcgcaacgt
      421 ttggcgcgat ttcgctcgac tgcgccagcg ccgggcgacg cagcgcggcg gcgcgtgcgc
      481 cggcatccag agcgtcccat tccaggatcg cgggttccgg cgcctgcatg gcgtcaatcg
      541 cggcattcat gccagcagct tctccaccgg caagaccagc aactggcttg cgccggcgcg
      601 acgcaagtct tccagatgct gccaggtgac cggaccgtcg cagaccgcct gcaaggcaat
      661 gcgtccgtcg tcgccctcga ccggcgtcac cgtcggcatc tgcggattcg gcagcagggc
      721 gttgatccgc tccaggctca cgcgatcggc gcgcagcatc accagcttgg actcgcgcac
      781 gcgcagcacg ccatcgatgc gccgcagcag cagctgcagc aactcgccgc gcgcatccgc
      841 gggcgcacag cgcgccccgg ccacgacggc ttcgctgtcg agcaacgtga agcgctcgct
      901 caactggttg gccatgagtg tcgcgccgct ggaaaccaga tcgcagacgg cgtccgcggt
      961 gccgaggcgt ggcgcgattt cgaccgaacc cgatagctcc acgatgcgcg catcgacgcc
     1021 ttgctgcgcg agccacgccc cgagcaggcc cggatacgag gtggcaatgc gcaatccgtg
     1081 caaatcctgc gccgtcgcgc aggccattgc ctgcggcacc gcaatgacga gtcggcaacg
     1141 ggcgcgatcc agtccgcgca gttcgacgat atcgccgcct atgccgatgc tgtgatgctc
     1201 ggccaggaca ttgcggccga cgatgccgat gtcgcacacg ccctgttcga tcaggcccgg
     1261 gatgtcgtcg tcgcgcacca gcagcaggtc gatcggcatg ctttcgccgt agcagaacag
     1321 cttgtctcgg ctttgccgga acttaaggcc gcagcgcgcc agcaggtctt gcgaaagatc
     1381 cgccagtcgg ccggactttt gcaccgccac gcgcaaacgt tcgcggtcga ccgcggcgct
     1441 cacggtcgcg gccttgacgt tgcctggcgc gcgcgtccac gcaaggcatc ggcggcgatg
     1501 gcggcacgat aaccgccatt gccctgggtg agggagcggg cgacccggcc gatcgtggtg
     1561 acgctgaccg cggtgtgcgc gtggatgtcg cggtaggcca tgccttgcag caggtacggc
     1621 acaacctgcc agcgatcggt catggcctca agctcggccg gcgtgcacag gtcgcgcagg
     1681 aacgcgagca tctgcgccgg cgtgcgcagg gaaagcaatg cgcgacacag gtcgtcttcg
     1741 gccgaagacg cggatttttc cggatcgagt tggcggcgct tcataatgta gtgtattaat
     1801 gcgttagtac gttaacgcat agaataaccc ggcggcgcgc gcaccgcaac cgcgcaaggc
     1861 cgcggggaag aaacgggaat accggagcgc gcgaaagcgc cgaacgatga tcaggcagcg
     1921 aggacgccct gcgcgaccag ttcgcacatt gcgcgcacgt cggcgtccat cgcgcagtcg
     1981 cggctg
//
LOCUS       DASRVO010000409         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_141407, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000409 DASRVO010000000
VERSION     DASRVO010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141407"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="rpsB"
                     /locus_tag="VFF05_05105"
     CDS             complement(<1..654)
                     /gene="rpsB"
                     /locus_tag="VFF05_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZW51215.1"
                     /translation="MPQVTMRQMLEAGVHFGHQTRYWNPRMAPYIFGARGKIHIINLE
                     KTLPLFTDAMNFVSGLAQRRGSILFVGTKRSARDSVKEEAVRCGMPYVSARWLGGMLT
                     NFRTVKQSVSRLKEMTAMAQDGSLERMSKKEALHYQREIAKLERSLGGIRDLESLPDA
                     LFVIDVGYQKGAVAEAKKLGIPVIAVVDTNHSPEGISYVIPGNDDSSRAIRLYARGVA
                     "
     gene            1127..1378
                     /locus_tag="VFF05_05110"
     CDS             1127..1378
                     /locus_tag="VFF05_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51216.1"
                     /translation="MSIRKLAQKVISARDIIADLAAARPPSQTRLLDARDRLDTAVES
                     IHALAEEMFLLQEQNQQLRRDLAERVCARICRKGYRGAS"
BASE COUNT          245 a          504 c          491 g          260 t
ORIGIN      
        1 ggccacgcct cgcgcgtaca gacggatggc acggctggaa tcgtcattgc cgggaatgac
       61 ataggagatg ccttcgggcg agtgattcgt gtccactacc gcgatgaccg ggatgccgag
      121 ctttttcgct tcggcgacgg cgcccttctg gtaacccacg tcgatcacga acaaggcgtc
      181 cggcagactt tccaggtccc ggatcccgcc gaggcttcgc tcgagcttcg cgatctcgcg
      241 ttggtagtgc agcgcttcct tcttggacat gcgctcgagg gagccgtcct gggccatcgc
      301 ggtcatttcc ttcaggcgcg aaaccgattg cttgaccgtg cggaagttgg tcagcatgcc
      361 gccgagccag cgcgcggaga cgtagggcat gccgcagcgc acggcttctt ccttgaccga
      421 atcgcgcgcc gagcgcttgg tgcccacgaa caggatcgag ccgcgcctct gcgccaggcc
      481 cgacacgaag ttcatcgcat cggtgaacag cggcagggtc ttttccaggt tgatgatgtg
      541 gatcttgccg cgcgcaccga agatgtacgg cgccatgcgc ggattccagt aacgggtctg
      601 atgaccgaag tggacgccag cttcgagcat ctggcgcatc gtgacttgag gcatggtgga
      661 actccggaaa aagggcggcc gcgaaccttg gtttcgcgcg gcaaagcccg gggttgagac
      721 ctccacgtct ccctcgcagc gaccccgcgg ccgtcgcgcc ggtcccttca gggtccgacg
      781 gatcagttgg ccggcaaggc accccgatgc gggtgtcgag acgtgtgcgg atttgctccc
      841 cacgatgggg aaccgcggaa ttgtagcgga ttccggcgct tgcgcgcaat cgctcgcccc
      901 ccgcaggtgc agggccttga agatcgaagg ggtcgccccc ggggcatccg agggtatgcg
      961 gccgctccgg ttcgctgcct cggcgcgcgg ctcctgccat tctttcaagc cggctcgcta
     1021 cgcgccgcag gccgccgctt gcggcacttg atctggctca acgcctgccg cggcagcggg
     1081 ctcaacatga aacggcggcg cagcgtccgc atcttcgcag gcggcaatga gcattcgcaa
     1141 gctggcgcaa aaggtgatct cggcgcgcga catcatcgcc gatctcgctg cggcgcgacc
     1201 gccgagccag acgcgcctgc tcgatgcccg cgaccgcctc gatacggccg tcgagagcat
     1261 ccatgccctg gccgaggaga tgttcctgtt gcaggaacaa aaccagcaac tgcgccggga
     1321 tctcgctgag agggtctgcg cacgcatctg ccgcaagggc tatcgcggcg cctcatagct
     1381 gccgggtgcc agcgcgcgcc cgccggccgc gcgcggcgac ggcgcaacgg gctttgcatc
     1441 ccggcttgca tccgcgcccg cgaacccgca ccttcgctac actatggccc ctgctggaca
//
LOCUS       DASRVO010000410         1282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_375180, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000410 DASRVO010000000
VERSION     DASRVO010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375180"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFF05_05115"
     CDS             <1..697
                     /locus_tag="VFF05_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HZW51217.1"
                     /translation="RRIEVQTDDDSIGGHILHLLHGQAPSELWVRSMHTSLILYAEHE
                     FNASTFAARVIAGTGSDMHSCIAGAIGALRGPKHGGANEAAFEIQKRYATPDEAEADI
                     RTRVERREVVIGFGHPVYTVSDPRNKVIKEVARELAAAQHNMKMFDIAERLESTMWNI
                     KKMFPNLDWFSAVSYHMMGVPTAMFTPLFVVSRTSGWCAHVIEQRIDGKIIRPSANYI
                     GPEDLKFVPLAER"
     gene            898..>1282
                     /locus_tag="VFF05_05120"
     CDS             898..>1282
                     /locus_tag="VFF05_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51218.1"
                     /translation="MTGCGRLTWSFVALFALAPALHAQVPEPLDRINLALGVFHPTVD
                     TRLSANASGLAGSDVDFHSDLGLDKHRTLPNLRLEFLAFDSQGFSIGGYEYSRNSSMR
                     LARDIRFGGIDYSVNAAVQSELRLQV"
BASE COUNT          221 a          431 c          423 g          207 t
ORIGIN      
        1 ccggcgcatc gaggtgcaga ccgacgacga ctcgatcggc ggccacatcc tgcatctgct
       61 gcacggccag gcgccgtcgg agctgtgggt gcgctcgatg catacctcgc tgatcctcta
      121 cgccgaacac gagttcaacg catcgacctt cgccgcgcgc gtgatcgccg gcaccggcag
      181 cgacatgcac tcgtgcatcg ccggtgcgat cggcgcgttg cgcggcccga agcacggcgg
      241 cgcgaacgag gccgcgttcg agatccagaa gcgctacgcg acgccggacg aggccgaggc
      301 cgacatccgc acgcgggtcg agcgcaggga ggtcgtgatc ggcttcggtc atccggtgta
      361 caccgtatcc gacccgcgca acaaggtcat caaggaagtc gcgcgcgaac tggccgcggc
      421 gcagcacaac atgaagatgt tcgacatcgc cgagcggctc gagtcgacca tgtggaacat
      481 caagaagatg ttcccgaacc tggactggtt cagtgcggtg agttaccaca tgatgggcgt
      541 gcccacggcg atgttcacgc cgctgttcgt ggtttcgcgc acgtcgggct ggtgcgcgca
      601 cgtgatcgag cagcgcatcg acggcaagat catccggccg agcgcgaact acatcgggcc
      661 ggaagacctg aaattcgtgc cgctcgcgga gcgctgaggg tacggcatcc gctgtccggc
      721 gcgcgcccgg gcgagcgtgg cgctggcgcg cgatgcacca caacgcacgc gtttgcgccg
      781 caggcgtcag gcagcgttca tccgcatcgg ttcgccgcgc cgtaagctgg cggctgtgat
      841 ccgcgcccgc gcgcggccca gtgtattcgt acggcagacg cagccaaagg cggcgctatg
      901 acaggatgcg gcaggctcac atggagtttc gttgcgctct tcgcgctggc gccggcactg
      961 cacgcgcagg ttcccgagcc actcgatcgg atcaacctgg cgctcggcgt gttccatccc
     1021 acggtcgata cgcggttgtc cgccaacgcg tccggcctcg ccggctccga cgtcgacttc
     1081 cacagtgacc tcggcctgga caagcaccgc acgctgccga atctgcgcct tgaattcctc
     1141 gccttcgaca gtcagggttt cagcatcggc ggctacgagt actccaggaa cagctcgatg
     1201 aggctcgcac gcgacatccg cttcggcggc atcgattaca gcgtgaacgc cgcggtgcag
     1261 tccgagctgc gcctgcaggt gc
//
LOCUS       DASRVO010000411         1653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_12112, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000411 DASRVO010000000
VERSION     DASRVO010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12112"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VFF05_05125"
     CDS             <1..568
                     /locus_tag="VFF05_05125"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660825.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZW51219.1"
                     /translation="KIAYRGAGTFEFLYEDGEFYFIQMNTRVQVEHPVTEMITGIDIV
                     QEQIRVAAGEKLQFRQKDIQIRGHAIECRINAEDPYRFTPSPGRITSYHAPGGPGIRV
                     DSHVYQGYTVPPNYDSMVGKVIAYGATREQAIRRMRVALSEMVVEGIQTNIPLHRELL
                     HDIRFLRGGVSIHYLEQKLAQEQKPKAK"
     gene            573..1463
                     /gene="prmA"
                     /locus_tag="VFF05_05130"
     CDS             573..1463
                     /gene="prmA"
                     /locus_tag="VFF05_05130"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZW51220.1"
                     /translation="MAWQSLTLDVEAAEAESMSDALLEQGVVSVCIEDADAGTDAEVP
                     RYAEPSWETPVAWRRHRLSVLLAPAMEPGAIIERAARAAGLAVAPAYRIDNIADDDWV
                     RRTQSQFGPVSIDGRLWIVPSWHEPPVHGGPVVRLDPGMAFGTGTHPTTRLVLQFLAR
                     EIRGGEHVLDYGCGSGILAIAAAKLGAGRAGAVDVDPQALQVTAENARANEVDLDVRA
                     PDALPAGDYDVIVANILSSPLILLAPLICSLTRVGGRLALSGILETQAAEVIAAYAPA
                     VELSIGGCAGGWILLEGKRR"
BASE COUNT          286 a          523 c          552 g          292 t
ORIGIN      
        1 caagattgcc taccgaggcg cgggcacctt cgaatttctc tacgaagacg gcgagttcta
       61 cttcattcaa atgaacacgc gcgtacaggt cgagcacccg gtcaccgaaa tgataaccgg
      121 gatcgacatt gtccaggagc agatccgggt cgcggccggc gagaaactgc aatttcgcca
      181 gaaagacatt cagatccggg gccacgcgat cgagtgccgg ataaacgccg aagaccctta
      241 ccgtttcact ccctcgccgg gacgcatcac gtcgtatcac gcccccggag ggcccgggat
      301 tcgcgtcgac tcgcacgtct accaaggcta caccgttccg cccaactacg attcgatggt
      361 cggcaaggtc atcgcgtatg gcgcgacgcg cgagcaggcg atccgccgca tgcgggtggc
      421 gctgtccgag atggtcgtcg agggcatcca gaccaacatt ccgctgcacc gcgaactgct
      481 tcatgacatc cgcttcctgc gcggcggcgt gtcgatccat tatctcgagc agaagctcgc
      541 tcaggagcaa aagcccaagg cgaagtagcg cgatggcgtg gcagtcgctc accctggacg
      601 tggaggcggc agaggccgag tcgatgtccg acgccctgct tgagcagggc gtggtgtcgg
      661 tctgcatcga agacgcggac gctggcaccg atgcggaggt gccgcgctat gcggagcctt
      721 cgtgggaaac gccggttgct tggcggcgtc atcgtcttag cgtgctgctc gcgccagcga
      781 tggagcctgg agcgatcatc gagcgcgcgg cacgtgctgc cgggctcgcg gtggcgcccg
      841 cgtaccgcat cgacaatatc gcggacgacg actgggtacg gcgtactcaa tcgcaattcg
      901 gacccgtgtc gatcgacggg cggctctgga ttgtgccgag ctggcacgag cccccggttc
      961 acggcgggcc ggtagtgcgg ctcgatccgg ggatggcgtt cggcaccggg acgcacccga
     1021 ccacgcggct tgtgctgcag ttcctggcgc gcgagattcg cggcggcgag cacgtgctgg
     1081 attacggatg cggttccggt atattggcga tcgcagccgc caagctcggc gccggccgcg
     1141 ccggcgcggt cgatgtcgac ccacaggcgc ttcaggtcac ggcggagaat gcgcgcgcga
     1201 acgaagtcga cctcgatgtg cgcgcgcccg atgcgctgcc cgccggtgac tacgacgtaa
     1261 tcgtggccaa catcctttcc agtccgctga tactgcttgc gccgctcatt tgttctctca
     1321 cgcgagtggg aggtcggctg gcactgtcag gcattctcga aacgcaggcc gcggaagtga
     1381 ttgccgccta tgcgcccgca gtcgaactgt cgatcggcgg gtgcgctggc ggctggatcc
     1441 tgctcgaggg caaacgccgg tgaacctgat cacgcgttgt ccaaaatgcg gtacggcgtt
     1501 tcgtattcag cctgcgcaac tggcggcgcg cggaggaagg gtgcgatgcg gcaagtgttc
     1561 gtcggtcttc gacgccgttg cggcgcttca ggatcacgcc ggcgcgccgg ccagtacgga
     1621 gccatcgccg cagctcggcc ttttcgaccc ctc
//
LOCUS       DASRVO010000412         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_89437, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000412 DASRVO010000000
VERSION     DASRVO010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89437"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..823
                     /locus_tag="VFF05_05135"
     CDS             290..823
                     /locus_tag="VFF05_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZW51221.1"
                     /translation="MALQIRRIVTGHDARGRSIFIIDDTPKMPSGRSSAGTSAVDLWE
                     TSATPADNSGNADPTDHPFRLPPPQQGSVLRVVEYPPDKERFAALRAKDWSADAERQG
                     YTRDASNARHAGFHKTNSVDYAIVLEGEIYALMDEGEKLMKAGDVLIQRGTSHAWSNR
                     SDKPARVAFVLIDAKPA"
     gene            864..>1538
                     /locus_tag="VFF05_05140"
     CDS             864..>1538
                     /locus_tag="VFF05_05140"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZW51222.1"
                     /translation="MQFPRVHLDFGAIRALPAELTALGITRPLLLTDRGLVACGVLAR
                     VHDAVTGRNDVVVFDEVPENPTVEGVNRAIDLYRREGCDGVVALGGGSVIDTAKAVAV
                     VAGHPGPITNYMGKPDSITHRVVPLIAIPTTAGTGSEASRGAGIHPDAKSRSNGLNSH
                     YIVPRVAICDPELTVSLPPRLTAGTGMDALSHCVEGFLTTSDNPPGKALALDGVRHVV
                     RFVERAV"
BASE COUNT          261 a          521 c          512 g          244 t
ORIGIN      
        1 gcgcgcgcgc aacgctgctt cggtcatcgg cggcgcggat gatcgaagag cggcactgca
       61 tccgggacag agagcatgcg gctgcggctt ccgccgcgcg cagcaccgct gcgtcggcgc
      121 tcgacaagcc ggtccaacat ccgtgctccc tccctgttgc gctccgcgaa cccgcatggc
      181 gatgcgggat tccgcgaacg acgcttgggc agattgcttg actgaccacg cttggtcaag
      241 cactctgccc cgcgagggaa agcgcgcgac gcttcgttcg gaggaaccca tggcgctgca
      301 gatcaggcgg atcgtcaccg gccatgacgc gaggggccgc tcgatcttca tcatcgacga
      361 tacgccgaaa atgccgagcg ggcgcagctc ggcgggcact tcggcggtcg atctgtggga
      421 aaccagcgcg acgccggccg acaattccgg caacgccgat ccgaccgatc atcccttccg
      481 cctgccgccg ccgcaacagg gcagcgtcct gcgtgtcgtc gaatatccgc ccgacaagga
      541 gcgctttgcc gcgctgcgcg ccaaggactg gtccgcggat gcggagcgcc aaggctatac
      601 gcgcgatgcc agcaatgcgc ggcacgccgg cttccataag accaacagcg tcgattatgc
      661 gatcgtgctc gagggcgaga tctacgcgct gatggatgaa ggcgagaagc tgatgaaggc
      721 aggcgatgtc ctcatccagc gcggcaccag ccatgcctgg agcaaccgtt cggacaagcc
      781 ggcgcgcgtc gccttcgtgc tcatcgacgc gaagccggcg taacggacgc ggcgcggtcg
      841 gagatggttc catgacactc ctcatgcaat ttccgcgcgt gcatttggat ttcggggcga
      901 ttcgagcatt gcccgccgag ctcacggcgc tcggtatcac gcggcctctg ctgctgacgg
      961 atcgcggcct tgttgcctgc ggcgtgctgg cgcgggtgca tgacgcggtg acggggcgca
     1021 acgacgtcgt cgtcttcgac gaggtgcccg agaatccgac cgtcgaagga gtcaaccgcg
     1081 ccatcgacct ctatcgccgc gaaggctgcg acggcgtcgt cgcgctgggc ggcgggtcgg
     1141 tgatcgacac ggcgaaggcg gtcgcggtcg tcgccggcca tcccggcccg atcaccaact
     1201 atatgggcaa gcccgacagc atcacgcatc gcgtggtgcc gctgatcgcc attccgacca
     1261 cggcgggaac gggaagcgaa gcctcgcgcg gcgccggtat ccatcccgac gccaagagcc
     1321 gctcgaacgg cctcaacagc cattacatcg tgccgcgggt cgcgatctgc gaccccgaac
     1381 tgaccgttag tctgccgccg cgtctcaccg cggggaccgg catggatgcg ctttcgcact
     1441 gcgtcgaggg gtttctcacc accagcgaca acccgcccgg caaggcgctg gcgctcgacg
     1501 gcgtgcgcca tgtcgtgcgc tttgtcgagc gcgcggtt
//
LOCUS       DASRVO010000413         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_324010, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000413 DASRVO010000000
VERSION     DASRVO010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324010"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VFF05_05145"
     CDS             <1..879
                     /locus_tag="VFF05_05145"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZW51223.1"
                     /translation="WGISAGHGLGNVLHTSERMMGEQKHSFWELFWPGLAANVGYWAT
                     LSLNIPDFTRFARSQRSQIIGQTIGLPLTMIAYSFLGIATTAATVVVFDKPVWNPVDL
                     VPMITHNPVILIVAMILILIAQISTNMAANVVAPSNDFSNVAPRYISFRTGGMITAVI
                     GVVMFPWAIFNSASAYIFTWLGGYGSLLGAIAGVMIADYWIVRRRQLDIADLYRVNGQ
                     YPRWNAIAFVAMAIAIVPVVPGFIAAATTPGGVVSNPGFVDSLYTYGWFFTFSVALVA
                     YWAMSVATGKSRAPRS"
     gene            complement(907..1344)
                     /locus_tag="VFF05_05150"
     CDS             complement(907..1344)
                     /locus_tag="VFF05_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HZW51224.1"
                     /translation="MDPFLQAAIDEARQGLKEGGIPIGSVIVHAGRILGRGHNRRVQK
                     GSTVLHGEMDAFENAGRQPAKVYRESVLYTTLSPCPMCSGAILLYGIPRVIIGENRTF
                     MGEEELLRSRGVSVEVLQDDACIAMMREFIAARPELWNEDIGV"
     gene            complement(1390..>1884)
                     /locus_tag="VFF05_05155"
     CDS             complement(1390..>1884)
                     /locus_tag="VFF05_05155"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZW51225.1"
                     /translation="AMDFGAGSLYQGSGEAYVVGNTIVDNGNDPGQNTGGLALGGNAH
                     FTLANNILWNNTADGGYDLYAGATHSRLHNDVGVAGGPHATPDIAQGNLSVDPGFADC
                     PLICVIPYVLASTSALINAGDDAPGNGIGTLDLEMQTRLVGSHVDIGAYENQNVIFRD
                     GFGP"
BASE COUNT          305 a          607 c          592 g          380 t
ORIGIN      
        1 tggggaattt ccgccggcca cggactcggc aacgtgctgc acacgtcgga gcgcatgatg
       61 ggcgaacaga aacattcctt ctgggaattg ttctggccgg ggctggccgc caatgtcggt
      121 tactgggcga cgttgagcct gaacattccc gacttcaccc gcttcgcgcg ctcgcagcgc
      181 tcgcaaatca tcggccagac gatcggcctg ccgttgacca tgatcgccta cagttttctc
      241 ggcatcgcca cgacggcggc gaccgtggtg gtattcgaca agccggtgtg gaatccggtc
      301 gatctggtgc cgatgatcac gcacaacccg gtgatcctga tcgtggccat gatcctgatt
      361 ctcatcgcgc agatcagcac caacatggcg gccaatgtgg tggcgccgtc caacgatttt
      421 tccaacgtcg cgccgcgcta catttccttc cgcaccggcg gcatgatcac cgccgtgatc
      481 ggcgtggtga tgtttccatg ggcgatcttc aacagcgcaa gcgcctacat cttcacctgg
      541 ctgggcggct acggcagcct gctcggcgcc atcgccggcg tgatgatcgc cgactactgg
      601 atcgtgcgcc gtcgccagct cgacatcgcc gacctgtatc gcgtgaacgg ccagtatccg
      661 cgctggaacg cgatcgcgtt cgtcgccatg gccatcgcca tcgttccggt cgtgccgggt
      721 ttcatcgccg cggcgaccac gcccggcggt gtcgtttcca acccgggttt cgtggatagt
      781 ctgtacacct acggctggtt cttcaccttc tccgtcgcgc ttgttgcgta ctgggcgatg
      841 agtgtggcaa ccggcaagtc gcgcgcgcca cgatcttgat cagttcagca ggttaatcga
      901 agttcgctat accccgatgt cctcattcca aagctccggc cgcgccgcga tgaactcgcg
      961 catcatcgcg atgcaggcgt cgtcctgcaa aacttcgacg ctcacgccgc gcgagcgcag
     1021 cagctcttct tcgcccatga aggtgcggtt ttcgccgatg atcaccctgg gtatgccgta
     1081 cagcaagata gcgccgctgc acatggggca gggcgacagc gtggtgtaga gcacgctctc
     1141 gcggtaaacc ttggcaggct gcctgccggc gttctcgaac gcgtccattt cgccgtgcag
     1201 aacagtcgaa cccttctgca cgcggcggtt gtggccgcgg cccaggatgc gtccggcgtg
     1261 cacgatcacc gagccgatcg gaataccgcc ttccttcaat ccctggcggg cttcgtcgat
     1321 ggcggcttgc agaaacggat ccattgcgtg ctccgaaagt gcggagcctc gactctagca
     1381 cgcgtggtgc tacggcccga aaccgtcgcg aaagatcaca ttctggtttt cgtaggcgcc
     1441 gatatccacg tgcgagccga cgaggcgcgt ctgcatttcg agatcgagcg tgccgatgcc
     1501 attacccggc gcatcgtcgc ccgcgttgat cagcgcggac gtgctggcga gcacgtaggg
     1561 aatgacgcag atcagcgggc agtccgcgaa gcccgggtcg acgctcaggt tgccctgcgc
     1621 gatgtccggc gtcgcgtgcg ggccgccggc gacgcccacg tcgttgtgca ggcggctgtg
     1681 cgtggcgccg gcatacaggt cgtagccgcc gtccgccgtg ttgttccaca ggatgttgtt
     1741 ggccagcgtg aaatgtgcgt tgccgccgag cgcgaggcca ccggtgttct ggccgggatc
     1801 gttgccgttg tcgacgatgg tgttgccgac gacgtaggcc tcgccgctgc cctggtagag
     1861 cgagccggcg ccgaagtcca tcgc
//
LOCUS       DASRVO010000414         1192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_141517, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000414 DASRVO010000000
VERSION     DASRVO010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141517"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFF05_05160"
     CDS             <1..222
                     /locus_tag="VFF05_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZW51226.1"
                     /translation="KEKAEVAVIQAYMPAQMTPAEVDAAIAAAIAESGATGPKDMGKV
                     MGLVKPKVAGRTDMGKLSESIKAKLAALA"
     gene            368..754
                     /locus_tag="VFF05_05165"
     CDS             368..754
                     /locus_tag="VFF05_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZW51227.1"
                     /translation="MSKPKPNVVLSELQTDVVRILWRHGETSVADVARILGEERGLKH
                     TTVATLLARLHKRGVLAQRREGRQLLYLALVSEAQVRRSMVSNLLGNLFGGDSRELVA
                     HLLRESELTAGDLDRLRKRLGTEKSS"
     gene            751..>1192
                     /locus_tag="VFF05_05170"
     CDS             751..>1192
                     /locus_tag="VFF05_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51228.1"
                     /translation="MSHDMLLAASWLLTVALHGGAALAIVYAFDRSGRLTRTWREALW
                     RCALFGGALTATLQLALAPSPFGPWRVATARPVVAESATHPIVAPPVSSLRSTSPQDA
                     RTTAAPDKVDSAALPAIANAPVVAAHPQLSAPSHIPVFALSALIG"
BASE COUNT          220 a          394 c          374 g          204 t
ORIGIN      
        1 aaggaaaaag cggaagtcgc cgtgatccag gcctacatgc cggcgcaaat gacaccagcg
       61 gaagtcgatg ccgcgatcgc cgccgcgatc gccgaatccg gcgcgaccgg tcccaaggac
      121 atgggcaagg tgatgggtct ggtcaaaccg aaagttgccg gccgcaccga catgggcaag
      181 ctgtccgaat cgatcaaggc caagctggcc gcgctcgcct gagcgccgcc cggcaccgaa
      241 tcgcatagtc gaaacgccgg gcgagcccga cgtccgtcct tcattaccgt cgccatgact
      301 tttgacctgt tgacgtttgc actttttact actacaaacg tagtactaca gctgtagaag
      361 tgtcgtgatg agcaaaccca aacccaatgt ggtcctgagc gaactgcaaa ccgacgtcgt
      421 gcgcatcctg tggcggcatg gcgagaccag cgttgccgac gtggcacgga ttctcggcga
      481 ggagcgcgga ctcaagcaca ccaccgtagc gacgctgttg gcgcggctgc acaagcgcgg
      541 cgtactcgcg cagcggcgcg aagggcgcca gcttctgtac ctcgcactgg tcagcgaagc
      601 gcaggtgcgg cgttcgatgg tctccaacct gctcggcaac ctgttcggcg gcgattcgcg
      661 cgaactggtg gcgcatctgc tgcgcgaatc cgaactcacg gcaggcgatc tcgatcgcct
      721 gcgcaaacgt ctcggcacgg agaagtcatc atgagccacg acatgttgct ggccgcgagc
      781 tggctgttga ccgtcgccct gcacggcggc gcggcgctgg cgatcgtgta tgcattcgat
      841 cgttccgggc gcctgacccg gacttggcgc gaagcactgt ggcgctgcgc cctgttcggc
      901 ggtgccttga ccgcaacgct gcaactggcg ctggcgccat cgccattcgg accctggcgc
      961 gtggccacgg cgcggccggt ggttgccgag agcgcgacgc acccgatcgt tgcgccaccg
     1021 gtgtcttcgc tgcgatcgac gtcgccgcag gatgcacgga ccaccgccgc tcctgacaaa
     1081 gtcgattccg ctgcattacc ggcaatcgcg aacgcgcccg tggtggcagc acatccacaa
     1141 ttgtcggcac cgtcgcacat acccgtcttc gcgctgtccg cactcatcgg ac
//
LOCUS       DASRVO010000415         1146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_220119, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000415 DASRVO010000000
VERSION     DASRVO010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220119"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1146
                     /gene="gpmI"
                     /locus_tag="VFF05_05175"
     CDS             <1..>1146
                     /gene="gpmI"
                     /locus_tag="VFF05_05175"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZW51229.1"
                     /translation="EVGHMNIGAGRIVYQDLTRIDAAIADGSFSRNPALLGACAAAKR
                     ASSEQATLHVFGLLSPGGVHSSEEHVFALLDMAKKQGVERVAVHAFLDGRDTPPQSAR
                     ASLEKLQARCDALGNARIATVSGRYYAMDRDKRWERVKLAYDAVAEGVAEFHADSALA
                     ALDAAYARGETDEFVKPTIIASATKIADGDAIVYMNFRADRARQLTQAFVDPDFSGFA
                     RTRTIRLSAFVTLTHYSDDLHVSAEAYPPQSLANSLGEYLSCLGMKQLRIAETEKYAH
                     VTFFFSGGREEPYPGEDRILVPSPKVATYDLQPEMSCPEVTEKLVAAIAGGKYDFIVC
                     NLANADMVGHSGKLDAAIKAVETVDAALGKLEVAVRAAGGEMLISADH"
BASE COUNT          220 a          382 c          350 g          194 t
ORIGIN      
        1 gaggtcggcc acatgaacat tggcgccggg cgcatcgtgt accaggacct gacccgcatc
       61 gacgcggcga tcgcggatgg cagtttctcg cgcaatccgg ccctgctcgg cgcctgtgcc
      121 gcggcgaaac gtgcttcatc agagcaggcg acgttgcacg tgttcgggct gctcagcccc
      181 ggcggcgtgc acagcagcga agaacatgtt ttcgccttgc tcgacatggc aaaaaagcag
      241 ggcgttgaac gcgtggccgt gcatgccttt ctcgacggcc gcgacacgcc gccgcagagt
      301 gcgcgcgctt ccctggaaaa attgcaggcg cgttgcgatg cgctcggcaa cgcgcgcatc
      361 gcaacggtca gcggccgcta ctacgcgatg gatcgcgaca agcgctggga gcgcgtgaaa
      421 ctcgcctacg acgctgtcgc tgaaggcgtc gcggaatttc atgcggacag cgcactcgcc
      481 gcactggatg ccgcctacgc gcgcggcgag accgacgaat tcgtcaagcc gaccatcatt
      541 gcaagtgcga cgaaaatcgc cgacggcgac gccatcgtct acatgaattt ccgcgccgat
      601 cgtgcgcgtc agctcacgca ggccttcgtc gatccggatt tttccggctt cgcgcgcacg
      661 cgcacgatcc gcctgtccgc cttcgtcacg ctcacgcact acagcgacga tctgcacgtc
      721 agcgccgaag cctatccgcc gcaatcgctg gccaattcgc ttggcgaata tctgtcctgc
      781 ctcggcatga aacagctgcg catcgccgag acggaaaaat acgcgcacgt cacgttcttc
      841 ttttccggcg gtcgcgagga accgtatccg ggcgaggatc gcatcctcgt gccgagtccc
      901 aaggtcgcga cctacgacct gcaaccggaa atgagctgtc ccgaagtgac ggaaaagctc
      961 gtcgctgcga tcgccggcgg caagtacgat ttcatcgtct gcaatctcgc caacgccgac
     1021 atggtcgggc acagcggcaa gctcgacgcc gcgatcaagg cggtcgaaac ggtcgatgcc
     1081 gcacttggca aactcgaagt cgcggtccgc gccgccggcg gcgagatgct gatcagcgcc
     1141 gatcac
//
LOCUS       DASRVO010000416         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_349264, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000416 DASRVO010000000
VERSION     DASRVO010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349264"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFF05_05180"
     CDS             <1..335
                     /locus_tag="VFF05_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZW51230.1"
                     /translation="DFDFSTLPQNPHGLAHLVVVDNPPQHPHGDFVLRDGLLHDDTAP
                     RLTFAGIGVYRPELLAAHAPGKFGIVPILRAAMREGRIGGTRHPGAWSDIGTPQRLAK
                     LDARLGSQ"
     gene            complement(321..1013)
                     /gene="hda"
                     /locus_tag="VFF05_05185"
     CDS             complement(321..1013)
                     /gene="hda"
                     /locus_tag="VFF05_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HZW51231.1"
                     /translation="MNAQLPLLLRWPAQQRFETFVTGTANRIALAVLQEAATTGAAHS
                     VFVSGPAGSGKTHLLLAACAAATAVGRTAQYLALGKLMDRAEAIRAFGGSDLLALDDV
                     HASAGDATAEHALFDLYNRCKVERSTLLFSASAQPAQIGIGLPDLVSRLSACTQARLT
                     PLDDAQRRDWLRARAGTRGLILDDGVLDWLFAHHARDLSSLAELLERMDRAALAAQRR
                     VTVPFLRDLLRT"
     gene            complement(1010..>2185)
                     /locus_tag="VFF05_05190"
     CDS             complement(1010..>2185)
                     /locus_tag="VFF05_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZW51232.1"
                     /translation="PAICGGVVGQGAARAQGGRMTTALRWQLLALTLLVGALLWLLAP
                     VLTPFAIAAMFAYLFDPLVARLQRLRLGRGAATAIVFLSLTLILILTLLWLIPYLQHQ
                     VSTLIRRLPDWIAWLQTDAVPWLNAKFDLEIDLPDVQQLTAVLQQHWKEAGGLAANIL
                     ATVSKSGFALLTWSVHVLIVPVAFFYLLRDWRSMIAQVHDLLPRSIEPVAVRLARESD
                     ETLGGFLRGQLSVMIVLGVLYAIGLFAIGLDVGPLIGIVAGLISFVPYLGAITGVLLG
                     AVAAFAQYHDWMHVVGVLIVFGVGHVVEGYVLVPRLVGEKIGLHPLAVIFAILAGGEL
                     FGFLGVLLALPIASVAMVVLRYLHERYRSSELYGSGREAAIVVADAPAREKSQPQQP"
BASE COUNT          419 a          729 c          728 g          309 t
ORIGIN      
        1 cggacttcga tttttccaca ttgccgcaaa atccacacgg gctcgcgcac ctggtcgtgg
       61 tcgacaatcc gccgcaacat ccgcatggcg atttcgtgct gcgcgatgga ttgctgcacg
      121 acgacaccgc gccgcgcctg acctttgccg gcatcggcgt gtatcgcccg gaattgctcg
      181 ccgcgcacgc gccggggaaa ttcggcatcg tgccgatcct gcgcgcggcg atgcgcgaag
      241 gccgtatcgg cggcacgcgc catcctggcg catggtccga catcggcacg ccgcagcggc
      301 tggccaaact cgacgcgcgt ctaggttcgc agtaaatcgc gcaggaacgg cacggtgacg
      361 cgccgctgcg cggccagtgc ggcgcggtcc atgcgttcaa gcaattccgc gaggctggac
      421 aagtcgcgcg catggtgcgc gaacagccaa tcgagcacgc cgtcgtcgag gatcagcccg
      481 cgcgtgcccg cgcgcgcgcg caaccagtcg cgccgctgcg cgtcgtcgag cggggtcaag
      541 cgcgcttgcg tgcaggcgga aaggcgcgaa accaggtccg gcaagccgat accgatttgc
      601 gcgggctgcg cggacgcgga aaacagcaat gtggatcttt ccaccttgca gcgattgtac
      661 agatcgaaca acgcgtgttc ggcggtcgcg tcgccggcgc tggcgtgcac gtcgtcgagc
      721 gcgagcaggt cgctgccgcc aaacgcacgg atcgcctccg cgcgatccat caattttccc
      781 agcgccaggt actgcgcggt gcggccgacc gcggtcgccg cggcgcaggc ggccaacagc
      841 aggtgcgtct tgccactgcc ggcaggtccg ctcacgaaaa ccgagtgcgc cgcgcctgtc
      901 gtcgccgctt cctggagcac ggcgagtgcg atgcgattcg ccgtgccggt cacaaacgtc
      961 tcgaatcgct gctgtgccgg ccagcgcagc agcaacggca actgtgcgtt catggctgct
     1021 gcggttgcga cttctcgcgc gcgggggcat cggccacgac gatggccgct tcgcgaccgc
     1081 tgccgtacag ctcgctggag cggtagcgct catgcagata gcgcagcacc accatggcca
     1141 ccgaggcgat tggcaaggcc agcagcacgc cgaggaatcc gaacagttcg ccaccggcga
     1201 ggatcgcgaa gatcaccgcg agcggatgca ggccgatctt ttcgccgacc aggcgcggca
     1261 ccagcacata gccttcgacg acatggccga cgccgaaaac gatcagcacg ccgacgacgt
     1321 gcatccagtc gtggtattgc gcgaacgcgg cgactgcccc gagcagcacg ccggtgatgg
     1381 cgccgaggta cggcacgaag ctgatcaggc cggcgacgat gccgatcaat ggcccaacat
     1441 ccaggccgat cgcgaacagg ccgatggcat agagcacgcc gaggacgatc atcaccgata
     1501 actgcccgcg caggaatccg ccaagggttt cgtcggattc gcgcgccagc cgcacggcaa
     1561 cgggctcgat ggagcgcggc agcaggtcgt ggacctgcgc gatcatgctg cgccaatcgc
     1621 gcagcaggta gaaaaaggcg accggcacga tcagcacgtg cacgctccag gtcagcagcg
     1681 cgaaaccgga cttggagacc gtggccaaaa tattggccgc cagtccgccg gcctccttcc
     1741 agtgctgttg caggaccgcg gtgagttgtt gcacgtcggg caggtcgatc tcgaggtcga
     1801 acttcgcgtt gagccagggc accgcgtcgg tctgcagcca ggcgatccag tcgggtaggc
     1861 gccggatcaa ggtggaaacc tgatgctgca agtagggaat cagccagagc aaggtcagga
     1921 tcaggatcag cgtcaaggac aaaaacacga tcgcggtggc ggcaccgcgg cccagtcgca
     1981 ggcgttgcag gcgcgcgacc agcggatcga acagataggc gaacatcgcc gcgatcgcga
     2041 acggcgtgag caccggcgcg agcagccaca acagcgcgcc gaccagcagc gtcagggcaa
     2101 gcaattgcca gcgcagcgcg gtcgtcatgc gcccgccttg cgcgcgcgca gcgccttgcc
     2161 cgaccacacc accacatatt gcagg
//
LOCUS       DASRVO010000417         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_12231, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000417 DASRVO010000000
VERSION     DASRVO010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12231"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFF05_05195"
     CDS             <1..483
                     /locus_tag="VFF05_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZW51233.1"
                     /translation="PEILQDFAGKRLDWYVTGWGTGGTLTGAGEMLKLARPDVKIAAC
                     EPEVAAMLSGKEWTPHKIQGWTPNLIPAVLNRKVVDRIITVDDTVARDTARALAQKEG
                     IFCGISSGATFAAALEVAKQARQGDVILAMLPDTGERYLSTFLFEGMNEGTDVELADL
                     "
     gene            complement(511..759)
                     /locus_tag="VFF05_05200"
     CDS             complement(511..759)
                     /locus_tag="VFF05_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51234.1"
                     /translation="MNLRWPEAIGLLGVVLILLAYFLLQVGRLRGNALPYQLLNAVGA
                     LLLLVSLFYAFNLSAFLMESAWLFVSIYGIVRNRRRRT"
     gene            complement(756..1502)
                     /gene="rlmB"
                     /locus_tag="VFF05_05205"
     CDS             complement(756..1502)
                     /gene="rlmB"
                     /locus_tag="VFF05_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZW51235.1"
                     /translation="MSGELIYGIHAIEAALTHDPGNILELYFESDSHNARLKELSDHA
                     REAGLKPHARDKAALDKMTGGARHQGAVARYRTPPPRSESELAALVEKAGAEALLLVL
                     DGVTDPHNLGACLRSAEAAGVTAVVVPKDKAAGITPTVRKASAGAADRIAFFAVTNLA
                     RTLKTLKQQGVWLAGLAGDVPQDFYSLDLKGPLAIVMGSEGEGMRRLTRESCDFLAKI
                     PMRGSVESLNVSVATGVVLFEALRQRGKKS"
     gene            complement(1499..3754)
                     /gene="rnr"
                     /locus_tag="VFF05_05210"
     CDS             complement(1499..3754)
                     /gene="rnr"
                     /locus_tag="VFF05_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZW51236.1"
                     /translation="MPEMPARPQSTSTRSGPRRGTKPSPRPADPQAEREAQRYAHPIA
                     SREAIVELLGARGELLTAEVLARELGLSDPRDLEALSKRLTAMLRDGQLLQNRRGGFG
                     VAQKLDLIPGTILANAEGFGFLRPDAGGDDIYLSPYEMRKVLHGDRVLASIVGVDHRG
                     RKQGAIVEVLERRASRLVGRVVTENGIVVVAPDDRRLHQDVLIPPGKQRDARAGQIVV
                     AEITQPPTAQRGPIGEIVSVLGERLVPSLIVEMAVASHGLPHEWPDEASREAAQVEPQ
                     VAREEIAGRVDLRKLPLVTIDGEDARDFDDAVFAEPTQEGFRLVVAIADVSHYVRPGT
                     ALDDEAVKRATSVYFPGFVVPMLPETLSNGICSLNPKVERLCMVCDMIVGHDGEVVRS
                     KFHPAVMRSHARLTYTRVWQALGEKEADARAELADVLPHLEHLYQLYRILAKARQRRG
                     AIDFDSREVKFRLDKAGDVVQLGAEPRNDAHKLIEECMIAANVEAAKFLDPRKIPALY
                     RDHAPPPESKYEDLLEFLREFHLKLPPAVQVQPKDFSSLLKKIRDRVDAGLIQSVLLR
                     SQSLAVYSAKNSGHFGLALDAYAHFTSPIRRYPDLLVHRAIRYALTKGKPSEYTYSPG
                     EMVNLATHCSQNERRADEAERDVDERYKCAWMEKHVGSEFDGVVSGVTSFGLFVELSE
                     SQVSGLVHITQLPNDYYHFDPVRHLLTGERRGLKFRLGDAVRVQVLRASLEDRKIDFR
                     LATEERGRKNK"
     gene            3901..3985
                     /locus_tag="VFF05_05215"
     tRNA            3901..3985
                     /locus_tag="VFF05_05215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3935..3937,aa:Leu,seq:cag)
     gene            4003..>4974
                     /locus_tag="VFF05_05220"
     CDS             4003..>4974
                     /locus_tag="VFF05_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZW51237.1"
                     /translation="MTPASAPSGNAHGTRDFRVVAAVSTAHMLNDIMQSVILALYPLL
                     KGEFDLGFFQIGAISLTFQLTASLLQPVVGTVTDKHPFPLILPIGMAFTMCGLLLLSV
                     APNYPVILLAVGLIGIGSSTFHPESSRVARLASGGQYGLAQSLFQIGGNTGQALGPTL
                     AAFLILRHGGAEARPHAAWFALLALLAMVILLQVSRWYGAHLKQRAAMHHAISASPFS
                     TGTVRRTLAILLALMFSKFFYLSSIGSYYTFYLIHQFGLSDHAAANLLAIFLFAVAAG
                     TLLGGPLGDRIGRRRVIWFSILGCAPFTLLLPHVGLGMTVALSVVIGL"
BASE COUNT          822 a         1649 c         1566 g          937 t
ORIGIN      
        1 ccggagatcc tgcaggactt cgccggcaag cgcctggact ggtacgtcac cggctggggc
       61 accggcggca cactcaccgg tgccggcgaa atgctcaagc tggcgcggcc cgacgtgaag
      121 atcgccgctt gcgagccgga agtcgcggca atgctctcgg gcaaggaatg gacgccgcac
      181 aaaatccagg gctggacgcc gaacctcatt cctgccgtgc tcaaccgcaa agtggtcgac
      241 cgcatcatca ccgtcgacga cacggtcgca cgcgataccg cacgcgcctt ggcacagaag
      301 gaaggcatct tctgcgggat ttcctcgggc gccacatttg ccgccgcgtt ggaagtggcc
      361 aagcaggccc ggcaaggcga cgtgatcctg gccatgctgc cggataccgg cgagcgttac
      421 ctgtcgacct tcctgttcga gggcatgaac gaaggcacgg atgtcgagtt ggcggatttg
      481 tagtcctgct gcagccgccg gcgcagcgat tcaagtcctg cgccggcgat tccgcacgat
      541 tccataaatg ctcacaaaca gccatgccga ttccatcagg aacgccgaca ggttgaaggc
      601 gtagaacagc gacaccagca agagcaacgc gccgaccgca ttgagcaact ggtacggcaa
      661 tgcattgccg cgcaagcgcc cgacctgcag aaggaaatac gcgagcagga tcaggacgac
      721 gcccaacaag ccgatggcct cgggccagcg cagattcacg atttttttcc gcgctgacgc
      781 agcgcttcga acagcaccac gccggtcgcc actgacacat tcaggctttc cacgctgccg
      841 cgcatcggaa tcttcgcgag aaagtcgcag gattcgcgcg taagccggcg catgccctcg
      901 ccttcgctgc ccatgacgat ggcgagcggg cctttcaaat cgaggctgta gaaatcctgc
      961 ggaacatcgc ccgccaatcc ggccagccac acgccctgct gcttgagcgt ctttagcgtg
     1021 cgcgccaagt tcgtcaccgc gaagaacgcg atgcggtcgg ccgcaccggc cgacgccttg
     1081 cgcaccgttg gcgtgatgcc ggccgccttg tccttgggca cgacgacggc ggtgacgccc
     1141 gcggcttctg cgctgcgcag gcaggcaccg aggttgtgcg ggtcggtcac gccgtcgagc
     1201 acgagcagca gcgcttcggc accggccttt tcgaccagtg ctgccaattc gctctcgctg
     1261 cgcggcggcg gcgtgcggta gcgcgcgacc gcgccctggt ggcgcgcgcc accggtcatc
     1321 ttgtccagcg ccgccttgtc gcgcgcgtgc ggcttgaggc cggcctcgcg cgcatggtcg
     1381 gacaattcct tcaagcgcgc gttgtggctg tcgctctcga aatacagttc gaggatattg
     1441 ccgggatcgt gcgtgagcgc cgcttcgatc gcgtggatgc cgtagatcag ctcgccgctc
     1501 atttgttctt ccttccgcgt tcctcggtcg ccaaccggaa atcgatcttg cgatcctcca
     1561 ggctggcgcg cagcacctgc acgcgcacgg catcgccgag acggaatttc aacccgcgcc
     1621 gttcaccggt cagcaggtgc cgcaccggat cgaaatggta gtagtcgttc ggcaattgcg
     1681 tgatgtgcac cagtccactc acctgcgatt cggacagttc cacgaacagg ccgaacgagg
     1741 tcacgcccga aaccacgccg tcgaattcgc tgccgacgtg cttttccatc cacgcgcact
     1801 tgtagcgttc gtcgacgtcg cgctcggctt cgtcggcacg gcgctcgttc tgggaacaat
     1861 gcgtcgcgag gttgaccatc tcgcccggcg agtaagtgta ttcggacggc tttcccttcg
     1921 tcagcgcgta gcggatcgcg cggtgcacga gcaggtcggg gtaacgccgg atcggcgagg
     1981 tgaagtgtgc atacgcatcc agggccaggc cgaaatgtcc ggaattcttc gcgctgtaca
     2041 cggccagcga ttgcgaacgc agtagcacgg attggatcag gccggcatcg acgcggtcgc
     2101 ggatcttctt cagcaggctg gaaaaatcct tgggctgcac ctgcacggcc ggtggcagct
     2161 tcaggtgaaa ttcgcgcagg aattcgagta ggtcctcgta cttggattcg ggcggcggcg
     2221 cgtggtcgcg gtacagcgcc gggatcttgc gcgggtcgag gaacttcgcc gcctccacgt
     2281 tcgcggcgat catgcattcc tcgatcagct tgtgcgcgtc gttgcgcggc tcggcgccga
     2341 gttgcaccac gtcgccggcc ttgtcgaggc gaaacttcac ctcgcggctg tcgaaatcga
     2401 tcgcgccgcg gcgctgccgt gccttggcca gaatccggta taactggtac agatgctcca
     2461 gatgcggcaa gacgtcggcg agttcggcgc gcgcgtcggc ctctttctcg cccagtgcct
     2521 gccacacgcg cgtataggtc aggcgcgcat gtgaacgcat caccgcggga tggaacttcg
     2581 agcgcacgac ctcgccgtcg tggccgacga tcatgtcgca aaccatgcac aggcgctcga
     2641 cttttggatt gagcgagcag atgccgttcg acaacgtctc cggcaacatc ggcacgacga
     2701 agccgggaaa gtacaccgac gtcgcgcgct tgaccgcttc gtcatcgagc gcggtgcccg
     2761 ggcgcacgta atgcgaaacg tccgcgatcg cgacgacgag ccggaatcct tcctgcgtcg
     2821 gctcggcgaa caccgcgtcg tcgaaatcgc gcgcatcctc gccgtcgatg gtgaccagcg
     2881 gcagcttgcg caggtccacc cgcccggcga tttcctcgcg cgcgacttgc ggctcgacct
     2941 gcgccgcttc gcgcgaggct tcgtccggcc attcgtgcgg caagccgtgg ctggcgacgg
     3001 ccatctcgac gatcagcgat ggcaccaggc gctcgccgag cacgctgacg atttcaccga
     3061 tgggaccccg ctgcgccgtc ggcggttggg tgatttcggc gacgacgatc tgcccggcgc
     3121 gcgcgtcgcg ctgcttgccc ggcggaatca gcacgtcctg atgcaggcgg cggtcgtccg
     3181 gcgccaccac gacgatgccg ttctcggtga cgacgcgccc gaccagccgc gacgcgcggc
     3241 gttcgagcac ttccacgatc gcgccctgct tgcgcccgcg atggtccacg ccgacgatgc
     3301 tggcgagcac gcggtcgccg tgcagcacct tgcgcatctc gtacggtgat agataaatgt
     3361 cgtcgccacc ggcatccgga cgcaggaaac cgaaaccttc ggcgttggcg aggatcgttc
     3421 cgggaatcag gtcgagtttt tgcgcgacgc caaagccgcc gcggcggttc tgcagcaact
     3481 ggccatcgcg cagcatcgcg gtcaggcgct tggacaaggc ctccagatcg cgcggatcag
     3541 acagacccag ctcgcgtgcc agcacttcgg ccgtgagcaa ttcgccgcgt gcacccagca
     3601 actcgacgat ggcctcgcga ctcgcgatcg gatgcgcgta acgctgcgcc tcgcgctcgg
     3661 cctgcggatc ggcgggacgc ggcgatggct tggtgccgcg tcgcggtccg gatctggtcg
     3721 acgtggattg cggacgcgct ggcatctccg gcatccagcc cggccgtttc ggcccggact
     3781 ttccctgtcc ggaacgagtt gtattcttgc gcttcactgc ggccttttcg tagtcttgac
     3841 cttgcgataa taacaggcgg ccggtatcat tcgcggccct gcgcaagcag acgcacacct
     3901 gcccaggtgg cggaattggt agacgcacta gtttcaggta ctagcgggta aaaccgtgga
     3961 ggttcgagtc ctctcctggg caccattgaa aagtcccccg acttgacacc agcaagcgca
     4021 ccctctggca acgcccacgg cacgcgcgat tttcgcgttg tcgccgcggt cagcacggcg
     4081 cacatgctca acgacatcat gcagtcggtg atcctcgcgc tgtatccgct gctcaagggc
     4141 gagttcgatc tcggcttctt ccagatcggt gcgatctcgc tgacctttca gctcaccgcg
     4201 tccctgctgc aaccggtggt gggcacggtc accgacaagc atcctttccc gttgatcctg
     4261 ccgatcggca tggcgttcac gatgtgcggc ctgctgctgc tttcggtagc gccgaactat
     4321 ccggtgattc tgctggcggt cggactcatc ggcatcggtt cgtccacttt ccatcccgaa
     4381 tcctcgcgcg tggcgcgcct tgcttccggc gggcagtacg gcctggcgca gtcgctgttc
     4441 cagatcggcg gcaataccgg acaggcgctc gggccgaccc tggcggcttt tctcatcctg
     4501 cggcatggcg gcgccgaagc gcgtccgcat gcagcctggt tcgccttgct cgccctgctg
     4561 gcgatggtga ttctgttgca ggtcagccgc tggtatgggg cgcacctgaa acagcgcgcg
     4621 gcaatgcatc acgcgatcag cgcatcgcct tttagcaccg ggacggtgcg ccgaaccctc
     4681 gctatcctgc tggcgctgat gttttccaag tttttttacc tatccagcat cggcagctac
     4741 tacacttttt atctgatcca tcaattcggg ttgtccgatc acgccgcggc gaacctgctc
     4801 gcgatattcc tgttcgcggt ggcagctggc accctgctcg gcggaccgtt gggcgaccgc
     4861 atcgggcggc ggcgcgtgat ctggttttcc atcctgggct gcgcgccgtt caccctgttg
     4921 ctgccgcatg tcgggctggg catgaccgtc gccctgagcg tcgtgatcgg cctg
//
LOCUS       DASRVO010000418         1509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_272278, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000418 DASRVO010000000
VERSION     DASRVO010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272278"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..>1509)
                     /locus_tag="VFF05_05225"
     CDS             complement(220..>1509)
                     /locus_tag="VFF05_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51238.1"
                     /translation="DCDFALLEGYDTSVLDPSSTYSQWCLAQTSNPTSCGPGGGGTPP
                     VGGGININFSNYSTFGSATFLRAPIAPAAAKVLHFYVYRKLLSTATLPASGAPVVMAA
                     LFSPLDLGENKLDDNVATGNNTLANVAPGVVSDSAYTAFTNAVTALQESTDSSTLTFD
                     ISDADSIESPGNLLHASVTLNLPNGIQVPVAADCGSATPITMLPVNRTCTIDIPLNNA
                     AFWDSAVALAYQGQFNNVATDTTDGTYAPGISASIQIVATDAQGKNGAPVSTPVHIHS
                     AKNDAPVVTVDAGQLPAMQDPNDSNNSYPTYSCSIGASDCGATFNIVTLNNSVTAVAG
                     PLAAFDELAAQTTAANDLQCGADGGATEVAFANAPVITLHSASLTQYDVAFQFATPLA
                     ASSSLCTVTMTDAEIPNFPNSESAATVQSTFRIVVNP"
BASE COUNT          273 a          445 c          512 g          279 t
ORIGIN      
        1 gagcaggttc agcgtatcgc gcaattccgc ttcgactgca cgcagcgtgg cgcgatcgcg
       61 cggcagcaag gcttgcaggc gcgcacgcca gggttcgtcg agatggctgc cgatccaggc
      121 gtcgaggccg gatgcggcgg aggcgaccgc gttcatgcgg ctactgtggc acgcgccagc
      181 ggccgcggca atgcggccgg ccaagcgcac gcgagaaatt tacggattga cgacgatgcg
      241 gaaggtcgac tgcaccgtgg cggccgattc gctgttcggg aaattgggaa tttcggcgtc
      301 ggtcatggtc acggtgcaca gcgacgaact ggccgccaat ggcgtcgcga actggaaagc
      361 cacatcgtac tgggtcagcg acgccgaatg cagtgtgatg acgggcgcat tcgcgaacgc
      421 tacctccgtc gcaccgccat cggcgccgca ttgcagatcg ttggccgcgg tcgtttgcgc
      481 agccagctcg tcgaatgcgg ccagaggacc ggccaccgcg gttacgctgt tgttcaaggt
      541 gacgatattg aacgtggcgc cgcaatcgct cgcgccgatc gaacaggaat acgtcggata
      601 ggaattgttg ctgtcgttcg gatcctgcat ggccggcaac tggcccgcat cgaccgtcac
      661 caccggcgca tcgttcttgg cgctgtggat gtgcaccggc gtggaaaccg gcgcgccatt
      721 cttgccctgc gcgtcggtgg ccacgatctg gatgctggcg gaaatgccag gggcgtaggt
      781 gccgtccgtg gtgtccgtgg ccacgttgtt gaactgtccc tgatacgcca gggcgactgc
      841 ggaatcccag aacgcggcgt tgttcaaggg aatgtcgatc gtgcaggtgc ggttgaccgg
      901 caacatcgtg atcggcgtgg ccgaaccgca atccgcggca accggcacct gaatcccatt
      961 cggtagattc agcgtgacgc tggcgtgcag aagattgccg ggggattcga tggaatcggc
     1021 gtcgctgatg tcgaacgtga gcgtgctgct gtcggtgctt tcctgcagtg ccgtcaccgc
     1081 attcgtgaac gccgtgtacg cgctgtcgct gacgacgccc ggcgccacgt tggccagcgt
     1141 gttgttgccg gtggcgacgt tgtcgtcgag cttgttctct cccagatcga gcggcgagaa
     1201 cagtgcagcc atgaccaccg gcgcgccgga tgcaggcaag gtcgctgtcg acaagagctt
     1261 ccggtagaca tagaagtgca acacttttgc cgcggccggc gcgatcggtg cccgcaagaa
     1321 cgtcgcagag ccgaaggtcg aataattcga gaaattgatg ttgatgcccc caccaaccgg
     1381 cggcgttccg ccaccgcctg gcccacagga ggtcgggttg ctggtttgag ccaagcacca
     1441 ctgtgagtat gttgaggacg gatccaggac cgaggtgtcg taaccctcca gcagcgcgaa
     1501 atcgcagtc
//
LOCUS       DASRVO010000419         1648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_64268, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000419 DASRVO010000000
VERSION     DASRVO010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64268"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VFF05_05230"
     CDS             <1..526
                     /locus_tag="VFF05_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HZW51239.1"
                     /translation="AFGTVSFYLVQRGDKYGLRVKDSEAPTRTHFLGIDYFPIDASWR
                     IEAKWEAYNPPHEVEEPNILGQVDKVVVPGAAVFERDGKTYRLEPVIETPGDTNLFLV
                     FGDKTNGRETYGAARFVYTEPPKDGKIVIDFNKAYNPPCAFTPYATCPLPTAQNRMKL
                     RVTAGEKKYRGTHE"
     gene            549..>1648
                     /locus_tag="VFF05_05235"
     CDS             549..>1648
                     /locus_tag="VFF05_05235"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HZW51240.1"
                     /translation="MGKSVVILGAQWGDEGKGKIVDLLTERVGAVARFQGGHNAGHTL
                     VIKGKKTVLHLIPSGILREGVLCLIGNGVVLSPAALQQEIAELEAQGVDVRSRLKISP
                     ATPLIMPYHIALDQAREKASGKNAIGTTGRGIGPAYEDKVARRGIRVADLMYPHELPD
                     KLRSVLDYHNFVLKHWLKADEIEYSKVLDEALEFGDYVKPMIDDVATTLHDLRRDGAN
                     ILYEGAQGSLLDIDHGTYPFVTSSNTTVGGAYAGTGVGASDIDYVLGICKAYATRVGG
                     GPFPTELNDATGEALRKRGNEFGATTGRPRRCGWIDLVALKRAVQISGINGLAITKLD
                     VLDGLSSIKVCIAYEYRGKRRELAPLDAAGWD"
BASE COUNT          336 a          523 c          519 g          270 t
ORIGIN      
        1 cgcgttcggc acggtcagct tctatctcgt ccagcgtggc gacaaatacg gcctgcgcgt
       61 gaaggacagc gaggcgccga cgcgcacgca tttcctcggc atcgattatt tccccatcga
      121 cgcaagctgg cgcatcgaag cgaaatggga ggcctacaat ccgccgcatg aggttgagga
      181 gccgaatatc ctcggtcagg tcgacaaggt cgtcgtgccg ggcgctgcgg tattcgagcg
      241 cgacggcaag acgtatcggc tggagccggt gatcgagacg ccgggcgaca cgaatttgtt
      301 cctggtcttc ggcgacaaga ccaatggccg tgaaacctac ggcgcggcgc gcttcgtcta
      361 caccgaaccg cccaaggacg gcaagatcgt gatcgatttc aacaaggctt acaatccgcc
      421 ctgtgcgttc acgccgtatg cgacctgtcc gctgccgacc gcgcaaaacc gcatgaagct
      481 gcgcgtgacc gcgggcgaga agaaataccg cggcacgcac gaataatcct gatcaaatcg
      541 gaagaatcat gggcaagtcc gttgtaatcc tgggtgcgca gtggggtgat gaaggcaagg
      601 gcaagatcgt cgatctgctc accgagcgcg tcggcgcggt cgcgcgtttc cagggtggcc
      661 acaatgccgg ccatacgctg gtcatcaagg gcaagaagac cgtcctgcac ctgattccat
      721 ccggcatcct gcgcgagggc gtgctgtgcc tgatcggcaa cggcgtggtg ctctcgccgg
      781 cggcgctgca acaggaaatc gccgaactcg aagcgcaggg cgtggacgtg cgttcgcgcc
      841 tgaagatcag tccggctacg ccactgatca tgccatacca catcgcgctg gatcaggcgc
      901 gcgagaaggc gagcggcaag aacgcgatcg gcaccaccgg acgcggcatc ggtccggcct
      961 acgaggacaa ggtcgcgcgt cgcggcattc gcgttgctga cctcatgtat ccgcacgaac
     1021 tgcccgacaa gctgcgcagc gtgctcgact atcacaattt cgtgctgaaa cattggctca
     1081 aggcggacga aatcgaatac agcaaagtgc tcgacgaggc gctggaattc ggcgattacg
     1141 tcaagcccat gatcgacgat gtcgccacca cgctgcacga tttgcgccgc gacggggcga
     1201 acattttgta cgagggtgcg caaggctcgt tgctcgacat cgaccacggc acctatccgt
     1261 tcgtcacttc gtccaacacc acggtgggtg gcgcgtacgc cggcaccggc gtcggcgcca
     1321 gcgacatcga ttacgtactc ggcatctgca aggcctacgc gacgcgcgtc ggcggcgggc
     1381 cgttcccgac cgaactcaac gacgccaccg gcgaagcatt gcgcaagcgc ggcaacgaat
     1441 tcggcgccac caccgggcgt ccacgccgct gcggctggat cgatctggtc gcgctcaagc
     1501 gcgcggtgca gatcagcggc atcaatggcc tggccatcac caagctcgac gtgctcgacg
     1561 gcctgtcgtc gatcaaggtc tgcatcgcct acgaataccg cggcaagcgc cgcgaactgg
     1621 cgccgctcga cgccgccggc tgggacga
//
LOCUS       DASRVO010000420         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_299006, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000420 DASRVO010000000
VERSION     DASRVO010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299006"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..160)
                     /locus_tag="VFF05_05240"
     tRNA            complement(84..160)
                     /locus_tag="VFF05_05240"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124..126),aa:Pro,seq:cgg)
     gene            complement(268..987)
                     /locus_tag="VFF05_05245"
     CDS             complement(268..987)
                     /locus_tag="VFF05_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HZW51241.1"
                     /translation="MKALLVSSALGLGLLLTAGAWAQAPAGATASCKDGTYYTGTTHK
                     GACRGHKGVDKWLDGGASVAAPESAAKPEKSKKPKKAATAEAAPATAAPAAAAPAGAI
                     AMCKDGTYFTGTTHKGACRGHKGVKEWLDGAAAAAPAARSAPVAAPAPTAAPVAPATP
                     AAAPAAKAATSKRTPPTPASQIAQKPGGGPGLVWVNSGTKVYHCQGDEWYGKTKEGSY
                     MSEAAAKAAGNHGSHGKDCGQ"
     gene            complement(1122..2159)
                     /locus_tag="VFF05_05250"
     CDS             complement(1122..2159)
                     /locus_tag="VFF05_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HZW51242.1"
                     /translation="MSETLIFTSADRIAIVAPHPDDESIATGGLIQAARAAGAAVRVI
                     VLTDGDANFWPQRWIEKRWRIDTVARARWGARRREEARAAMRVLGLDAGDTAFFGFPD
                     LGLTDLLMHADPKVPAALREEFVAFAPTCVVLPALGDRHPDHSAANILVRLALQDAGA
                     KFPRLLEFAVHGGVAEGDAVCDLSDTQRRCKRAAILAHATQMRLSGKRFLRHAGAAEV
                     FRAAAADAVENPRIPLHARAEGDGRLRVELDAELARRGFSVFVVMDGGKRSCRLLVPW
                     KSAAAELPVFDAMQGKLARAARVEHAGRRVALVIESGCAQWRLGFVKLARPAPGFWVF
                     DRHGWQAIVRS"
     gene            2341..3690
                     /locus_tag="VFF05_05255"
     CDS             2341..3690
                     /locus_tag="VFF05_05255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW51243.1"
                     /translation="MHTQVLPPPAAVPDYHLQPGDAFYRIAILVGFVVVVVATWKLRA
                     FHGLLHSAHQYGWLGYVARPSLLWAGMGLLMLLFRTLLWFRYRTPPVATMEDAPMLTV
                     IIPAFNEGEMVARSIESVATARYPRDKLEIFVIDDGSRDDTWTHIERIAARFPGLVTT
                     LRFAQNRGKRAALEAGFRRGKGEVMVTIDSDSVIDPDTLLAMAGPFRDPKVGAVAGKV
                     SVYNRAAGIIPRMLNVRFVLSFDFLRAAQSTYGTVYCCPGALAAYRTEAVRRVLERWV
                     AQEFLGKRCTFGEDRAMTNDILDAGYDSVYQRAGVVHTIVPVAYKKLCKMFLRWDRSY
                     VREEMRFLGRIVWKRPRRARAIALADSLVTSTRHPVGIAAMLLLVATMFDHPDMLLRF
                     ACAMLLIAAFNMLYYLRAERSWDFIYGIGYSFYSVFALSWIFPYAVCTARKQGWLTR"
     gene            complement(3680..>4774)
                     /locus_tag="VFF05_05260"
     CDS             complement(3680..>4774)
                     /locus_tag="VFF05_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014647782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZW51244.1"
                     /translation="LAQDFKSGAFVLDERFEDGHSAIESRLTERLGDAGRRVHTGRSR
                     NDQVLVVTRLWLKEKLTLLQTQCRDIAQVSLDRAAGESLPLPGYTHLQRAVVSSTAMW
                     FAGFAEGFIDNAFRARDTLAWIDANPLGSAAGYGVNLKLDREHTTRALGFARMQVSPV
                     CAQLSRGKFEIAVLEVFGSALLDLRRLAWDLSLFSTAEFGFVALPDEYTTGSSIMPNK
                     RNPDVVELLRASYACVAAARCEIENLLSLPSGYQRDLQFCKGSIFRGVRHGLMALELV
                     PDLLARLQWNEATMRTAITPQMYATDVAIEQAAAGVPFRDAYRKAASATANAGEGRTP
                     ESSLAARTSPGAAADLRMDELRARLDVLSA"
BASE COUNT          820 a         1625 c         1457 g          872 t
ORIGIN      
        1 catgaaatcc tcgcggaact tttaccgaag cagtctgcca caagcaaaaa aaccggcgct
       61 cggccggttt cttgttcgat cattggtcgg ggtgagagga ttcgaacctc cgacttctac
      121 ctcccgaaag tagcgctcta ccaggctgag ctacaccccg acacggaaag caaaacgagc
      181 cgcgcattct agccgcgcga ccgccctggc gcaagacgaa aaacgcccgg cgagccgggc
      241 gtttttcgtt tccgatggct tcggaattta ctggccgcaa tccttgccgt gcgagccgtg
      301 gttgccggca gccttggccg ctgcttcgga catgtacgag ccttccttgg tcttgccata
      361 ccattcatcg ccctggcagt ggtaaacctt cgtgccggaa ttgacccaga ccaggcccgg
      421 tccgccaccg ggtttctgcg cgatctgcga ggccggcgtc ggcggggtgc gcttggacgt
      481 ggccgccttg gccgccggtg cggctgcggg cgttgccggg gcaactggcg cggcagttgg
      541 cgcaggtgcg gcgacgggag cactccgagc agccggcgca gccgcagcgg cgccgtcgag
      601 ccattccttc acgcccttgt ggccgcggca tgcacccttg tgcgtggtgc cggtgaagta
      661 tgtaccgtcc ttgcacattg ctatagcacc tgccggcgca gccgctgcag gcgctgccgt
      721 tgccggcgca gcctcagccg ttgcggcttt cttcggcttc ttcgattttt ccggcttcgc
      781 cgccgattcg ggcgcagcca cggacgcgcc gccatcgagc catttgtcca cgcccttgtg
      841 gccgcggcag gcacccttgt gcgtggtacc ggtgtaatac gttccgtcct tgcaggatgc
      901 cgtcgctcca gcgggtgcct gtgcccaggc gccagcggtc agcagcagtc ccaatcccaa
      961 agcgctcgaa acaagcaatg ctttcatcgc agtctccatt tgatggggaa tcctctggcg
     1021 catacgaatg tgcgcggtgc cgagaatccg cgcaaccctg gcgtgtgtca atcatctgcg
     1081 gcggtttccg gaatgccgtt cagccgtttc cgcacactcg ttcagcttcg cacgatggcc
     1141 tgccacccgt gccggtcaaa aacccagaat cccggcgccg gacgcgccag tttgacgaag
     1201 cccaggcgcc attgcgcgca tccggattcg atcaccaacg cgacccgcct gcccgcatgt
     1261 tcgacccttg ccgcgcgggc caatttgccc tgcatggcgt cgaagacagg caattcggca
     1321 gcagcggatt tccaaggcac cagcaaacgg caagagcgct tgccgccatc catgacgaca
     1381 aacacactga aaccgcgtcg ggccagctcg gcatccaatt ccacacgcaa gcggccatca
     1441 ccttcggcgc gcgcatgcag cggaatgcgc ggattttcga ccgcatcagc cgcagccgcg
     1501 cggaaaacct ccgcggcgcc ggcgtgacgc agaaagcgct taccgctcag gcgcatctgc
     1561 gtggcgtggg cgaggatcgc cgcgcgcttg catcgacgtt gcgtgtcgct caaatcgcat
     1621 acggcatcgc cttccgccac gcctccgtgc acggcgaatt cgagcaggcg cggaaatttc
     1681 gcgccggcgt cctgcaacgc gagacgcaca aggatgttgg ccgcgctgtg atcgggatgg
     1741 cggtcgccca gcgccggcaa cacgacgcag gttggcgcga aggcaacgaa ctcctcgcgc
     1801 agcgccgccg gcaccttcgg gtcggcgtgc atcagcaggt cggtcaagcc caagtccgga
     1861 aagccgaaaa acgccgtgtc gccggcatcg aggcccagca cgcgcatggc ggcgcgcgct
     1921 tcctcgcgcc ggcgcgcgcc ccagcgcgca cgcgcgacgg tgtcgatgcg ccagcgcttc
     1981 tcgatccagc gctgcggcca gaagttggcg tcgccatcgg tcagcacgat cacgcgcacc
     2041 gccgcgccgg cggcgcgcgc cgcctggata agccccccgg tggcgatgga ctcgtcgtcc
     2101 gggtgcggcg cgacgatcgc aatccgatcc gcggaggtaa aaatcagcgt ttcgctcaag
     2161 aacctggcgc cttggattgc atgcgacaac gatagtctgc gacggcggcg cagcgcaacc
     2221 acatgggctc gttaagcgtc gtttaacccc gattccgcga ttgtcaggta tctttgcgcg
     2281 cgctgcattc cagcacgtcc ctgaacccgc ccgcgagcgg tcgcgattcg agcaaagccc
     2341 atgcataccc aagtcctgcc gcctcctgcg gcggtacccg attaccacct gcagccgggc
     2401 gacgcgtttt accgcattgc catactggtc gggtttgtcg tcgtcgtcgt ggcgacctgg
     2461 aagctgcgcg cgttccacgg cctgctgcat tcggcgcacc agtacggttg gctgggctat
     2521 gtcgctcgac cgagtctgct ctgggccggc atgggtcttc tcatgctgct gtttcgcacc
     2581 ctgctctggt tccgttatcg cacgcctccg gttgcgacca tggaagatgc accaatgctc
     2641 accgtgatca ttcccgcctt caacgaaggc gagatggtcg cgcgttccat cgagtccgtt
     2701 gcgaccgcgc gctatccgcg cgacaaactc gaaatcttcg tcatcgacga cggcagccgc
     2761 gacgacacct ggacgcacat cgaacgcatc gccgcccgct tccccggcct ggtgacgacg
     2821 ttgcgcttcg cccagaatcg cggcaagcgt gccgcgctcg aagccggatt ccgccgcggc
     2881 aagggcgaag tcatggtgac catcgactcc gacagcgtga tcgatcccga tacgctgctc
     2941 gccatggccg gcccgttccg cgatcccaag gtcggcgccg tggccggcaa ggtcagcgtt
     3001 tacaaccgcg ccgccggcat cattccgcgc atgctcaacg tgcgtttcgt gctgtctttc
     3061 gatttcctgc gcgccgcgca gagcacctac ggcaccgttt actgctgtcc gggcgcgctc
     3121 gccgcctacc gaaccgaagc ggtacgccgc gtgctcgaac gctgggtcgc gcaggaattc
     3181 ctcggcaagc gttgcacgtt cggcgaggat cgcgccatga ccaacgacat cctcgacgcc
     3241 ggctacgaca gcgtctatca gcgcgcgggt gtcgtccaca ccatcgtgcc ggtggcgtac
     3301 aagaaactgt gcaagatgtt cctgcgctgg gatcgcagct atgtgcgcga ggaaatgcgt
     3361 tttctcggcc gcatcgtgtg gaagcgtccg cgtcgcgcgc gcgcgatcgc gctggccgat
     3421 tcgctggtga ccagcacacg ccacccggtc ggcatcgccg cgatgctcct gctcgtggcg
     3481 acgatgttcg atcacccgga catgctgctg cgcttcgcct gcgcgatgct gctgatcgcc
     3541 gcgttcaaca tgctctatta cctgcgcgcc gaacgctcct gggatttcat ctacggcatc
     3601 ggctattcgt tctattcggt cttcgcgctg tcgtggattt tcccgtatgc ggtgtgcacg
     3661 gcgcgcaagc agggctggct cacgcgctga gcacatcgag ccgcgcgcgc agttcgtcca
     3721 ttcgcaaatc ggctgccgca ccgggcgacg tgcgcgcagc caggctcgac tccggcgtcc
     3781 tgccctcgcc cgcattcgcg gtcgcgcttg cggccttgcg ataggcatcg cggaatggaa
     3841 ctcctgccgc ggcctgttcg atggccacgt cggtggcgta catctgcggc gtgatcgccg
     3901 tgcgcatcgt cgcttcgttc cactgcaaac gcgccaacaa gtccggtacc agctccagcg
     3961 ccatcaaacc atggcgcacg ccgcggaaga tcgaaccttt gcagaattga agatcgcgct
     4021 gataacctga tggcagcgac agcaggtttt cgatttcgca gcgtgccgcg gcgacgcagg
     4081 cgtagctggc acgcaacaat tccaccacgt cgggattgcg cttgttcggc atgatcgagc
     4141 ttccggtcgt gtattcgtcc ggcagtgcca cgaagccgaa ttcggcagtg ctgaacaacg
     4201 ataaatccca ggcaaggcga cgcaggtcga gcaaggcact gccgaaaact tccagcacag
     4261 cgatctcaaa tttaccgcgc gagagctggg cacaaaccgg cgacacctgc atgcgcgcaa
     4321 agccgagcgc gcgcgtggtg tgttcgcgat ccagtttcag gttcacgcca tagccggcgg
     4381 cgctgccgag cggattcgca tcaatccacg ccagcgtgtc gcgcgcgcga aacgcgttgt
     4441 cgatgaaccc ttccgcgaat cccgcgaacc acatcgccgt ggacgaaacc accgcgcgct
     4501 gcaaatgcgt gtatccgggc aatggcaacg attcgcccgc agcgcgatcc aggctgacct
     4561 gcgcgatgtc gcggcactgc gtttgcagca gcgtgagctt ttccttcaac cacaaccgcg
     4621 tcacaacgag cacctgatcg ttgcggctgc gcccggtatg cacgcgacgg ccggcgtcgc
     4681 cgaggcgttc ggtcaaacgc gattcgatgg cggaatggcc atcttcaaag cgctcatcga
     4741 gcacgaaggc gccgcttttg aaatcctgcg ccag
//
LOCUS       DASRVO010000421         1555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_116262, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000421 DASRVO010000000
VERSION     DASRVO010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116262"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="VFF05_05265"
     CDS             complement(<1..340)
                     /locus_tag="VFF05_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51245.1"
                     /translation="MTDAATAAQFTFVEHWQTENAENAEAVLEFWRRENAISDEASAK
                     KRLSEIVLHACDRNGSIAGVATAVKITLPRLGQPTYYFRCFIGKDWRKSRLVMQLLRR
                     TGEVLETYARA"
     gene            complement(337..>1555)
                     /locus_tag="VFF05_05270"
     CDS             complement(337..>1555)
                     /locus_tag="VFF05_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR03032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03032 family protein"
                     /protein_id="HZW51246.1"
                     /translation="TEQPEGLPGTGYTPQPDPKAEKKTVEEVLGSVHTSNFPDLLREL
                     GGCLIVSTYQAGKLVILRPDGNSINTHFRTFNRPMGMAADRQRLMLGALMEIVEFRNM
                     PDVAKRLHDPPRHDAVYMARRGHITGAIDIHEMAWDADGAIWFVNTAFSCLCTLDAKS
                     SFVPRWRPKFVSHYAPEDRCHLNGLAMRNGIPRYVTALGETNTREGWRVNKRDGGLII
                     DITTNRVVTRGLSMPHSPRWYDNRLWVLESGRGALCTVDPKTGERHDIARVPGFCRGI
                     DFIGPVAFIGLSQLRGTTPFTDIPITDDNAERLSGVWAVHIGTGKTIAFLKFTGGVQE
                     IFAVQSVQGVLFPEIVHEGELLGSSYALPDKALKEVGFSPPPEPKPETPTSGYVNPDP
                     PAPVSVPATEPTP"
BASE COUNT          244 a          461 c          552 g          298 t
ORIGIN      
        1 gcgcgcgagc gtaggtttcg agaacctcgc cggtgcggcg cagcagttgc attacgaggc
       61 gcgatttacg ccaatccttg ccgatgaagc agcggaagta atacgtgggc tgacccaggc
      121 gcggcaatgt gatcttcacg gctgtggcga cgccggcgat cgagccgttg cggtcgcaag
      181 cgtgcaacac gatttcgctc aggcgctttt tcgccgaggc ctcgtcggag atcgcgttct
      241 cgcggcgcca gaattccaga acggcttcgg cgttttcggc gttttccgtc tgccagtgtt
      301 cgacgaaggt gaattgcgct gcggtggcgg cgtcggtcat ggagtgggct cggtagcggg
      361 gacggaaacg ggtgcgggcg gatcgggatt gacgtagccg gaagtgggcg tttccggctt
      421 tggttcgggc ggcggcgaga agccgacttc cttgagtgcc ttgtccggca gggcatagga
      481 gctgccgagc agttcgccct catgcactat ttcggggaac agcacgccct gcaccgattg
      541 cacggcgaaa atttcctgca cgccgccggt gaacttgaga aaggcgatgg tcttgccggt
      601 accgatgtgc acggcccaca cgccggacag gcgttcggca ttgtcgtcgg tgatcgggat
      661 gtcggtaaat ggcgtggtgc cgcgcaactg cgacaggccg atgaacgcga ccggtccgat
      721 gaaatcgatg ccgcggcaga atcccggcac gcgcgcgatg tcgtgacgct cgccggtctt
      781 gggatcgacc gtgcacagcg cgccgcgccc ggattcgagc acccacaggc ggttgtcgta
      841 ccagcgcggc gaatgcggca tcgacagccc gcgggtgacc acgcggttgg tggtgatgtc
      901 gatgatgagt ccgccgtcgc gcttgttcac gcgccagcct tcgcgcgtgt tcgtctcgcc
      961 cagcgcagtg acgtagcgcg ggatgccgtt gcgcatggcc aggccgttca ggtggcagcg
     1021 gtcttccggc gcgtagtggc tgacgaactt cggccgccag cgcggcacga agctggattt
     1081 cgcatcgagc gtgcacaggc acgagaacgc ggtgttgacg aaccagatgg caccgtccgc
     1141 atcccaggcc atttcgtgga tgtcgatcgc gccggtgatg tggccgcgcc gcgccatgta
     1201 caccgcgtcg tggcgcggcg ggtcgtgcag gcgcttggct acgtccggca tgttgcggaa
     1261 ctcgacgatc tccatcagcg cgcccagcat caggcgctgg cggtcggcgg ccatgcccat
     1321 cggccggttg aaggtgcgga aatgcgtgtt gatcgagttg ccgtctgggc gcaggatgac
     1381 cagcttgccg gcctggtacg tgctcacgat caggcagccg ccaagttcgc gcagcaggtc
     1441 cggaaagttc gacgtgtgca cgctgccgag tacttcctcg acggttttct tctccgcctt
     1501 gggatcgggc tgcggcgtat aaccggtgcc gggcaggcct tcgggctgtt cggtc
//
LOCUS       DASRVO010000422         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_245997, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000422 DASRVO010000000
VERSION     DASRVO010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245997"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFF05_05275"
     CDS             <1..439
                     /locus_tag="VFF05_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HZW51247.1"
                     /translation="KLCEAGRFGQKTGAGWYRYAPGKRDPIADPLTQKLIDEYRAANG
                     ITPRTISDDEVVERCIYALVNEGARILAEGIAARASDIDLAYLNGYGFPAYCGGPMLY
                     ADMVGLPNVVRALKRFAAEPGADPSWQPAPLLLQLAEAGKTFN"
     gene            521..>1236
                     /locus_tag="VFF05_05280"
     CDS             521..>1236
                     /locus_tag="VFF05_05280"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZW51248.1"
                     /translation="MTDVAIVSTARTGLAKSWKGAFNMTYGATLAAHSIEHAVKRARI
                     DPAEVEDVILGAAALEGTTGGNVARVAALRAGLPVTTTGVSINRFCSSGLQAIAMAAQ
                     RIIVDNVPVMVAGGVESISCVQNEANRHMRADPAMLERKPEIYWSMLQTAENVARRYQ
                     IPKDKQDEYGVRSQLRAAAAQAAGKFDDEIAPITVQAGVADPVMGLRTKEVTVSKDEG
                     TREGTTKEGISSIKPALPGGS"
BASE COUNT          226 a          433 c          419 g          158 t
ORIGIN      
        1 caagctgtgc gaagcgggcc gcttcggaca gaagaccggc gccggctggt accgctacgc
       61 gcccggcaag cgcgacccca ttgcggatcc gctcacgcag aagctgatcg acgagtaccg
      121 cgccgccaac gggatcacgc cgcgcacgat cagcgacgat gaagtcgtcg agcgctgcat
      181 ctacgcgctg gtcaacgaag gcgcgcgcat cctggccgaa ggcatcgcgg cgcgcgccag
      241 cgacatcgac ctggcgtacc tcaacggcta cggcttcccc gcgtactgcg gcggtccgat
      301 gctctacgcc gacatggtgg gcctgcccaa cgtggtgcgc gcgctcaagc gcttcgccgc
      361 cgagcccggt gccgacccgt cgtggcaacc ggccccgttg ctgctgcagc tggccgaagc
      421 cggcaagacg ttcaattgac gcccgaaagg ccgcgtcagc ggccgccggg cgtcatcccc
      481 gcgcaggcgg ggatccatct cggatctgga aaggcaagac atgactgacg ttgcaatcgt
      541 ctccaccgcg cgcaccggcc tggccaagag ctggaagggc gctttcaaca tgacctatgg
      601 cgcgacgctg gccgcgcatt cgatcgagca cgcggtcaag cgcgcccgca tcgatcccgc
      661 cgaagtcgag gacgtgatcc tcggcgccgc ggcgctggaa ggcacgaccg gcggcaacgt
      721 ggcgcgcgtc gccgccttgc gcgccggcct gccggtcacg accacgggcg tctcgatcaa
      781 ccgcttctgc tcctcggggc tgcaggcgat cgcgatggcc gcgcagcgca tcatcgtcga
      841 caacgtgccg gtgatggtcg ccggcggcgt cgagagcatc tcctgcgtgc agaacgaggc
      901 caaccgccac atgcgcgcgg acccggcgat gctggagcgc aagccggaga tctactggtc
      961 gatgctgcag accgcggaaa acgtcgccag gcgctaccag atcccgaagg acaagcagga
     1021 cgaatacggc gtgcgcagcc agttgcgtgc cgccgccgcg caggccgccg gcaagttcga
     1081 cgacgagatc gcgccgatca ccgtacaggc cggcgtcgcc gatccggtca tgggtctgcg
     1141 cacgaaggaa gtgacggtca gcaaggacga aggcacgcgc gaaggcacga ccaaggaagg
     1201 catcagcagc atcaagccgg cgctgcccgg cggctc
//
LOCUS       DASRVO010000423         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_349510, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000423 DASRVO010000000
VERSION     DASRVO010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349510"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFF05_05285"
     CDS             <1..311
                     /locus_tag="VFF05_05285"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HZW51249.1"
                     /translation="LIWGFFVSTVVLFHATVTINSLAHRFGSRRFPTRDNSRNNWLLA
                     LLTFGEGWHNNHHHFPGAARQGFRWWELDVTYYLLRGLAACGLVWDLKPVPSGLKRAH
                     "
     gene            315..1559
                     /locus_tag="VFF05_05290"
     CDS             315..1559
                     /locus_tag="VFF05_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW51250.1"
                     /translation="MRIAVVGSGIAGLASAWLLSREHEVTLYEANDYLGGHTHTHDVE
                     IGGRDYAVDTGFIVFNPPHYPLLHKLFDALGVASQPTTMSFSVKDERCGLEYNATDLS
                     GLFCQRRNLVSPRFWRMLGDIRRFYREAPALLEHDGAGPDLGEYLAANRYSDVFRDDH
                     LVPMASALWSSPSALVLKFPVKYLARFMANHQMLHVRGRPQWRVVKGGSQAYVRALRT
                     GWRVRERIGDAVNRITREPHQVRIDSISGSASYHHAVLACHSDQALGLLGDANERERE
                     ILGAMPYQENDTVLHTDASLLPRRRRAWAAWNAIVPTDAGEQCTVSYCMNLLQSLDCP
                     EPIVVTLNRTAQIAPQKILKRLRYHHPVYTQASVAAQQRKAEIQGAKRTWFAGAYWGW
                     GFHEDGMRSAVDVANGLGVHWP"
     gene            1556..2329
                     /locus_tag="VFF05_05295"
     CDS             1556..2329
                     /locus_tag="VFF05_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HZW51251.1"
                     /translation="MSAPLASCLYEGWVRHRRHAPHAHAFRYRMYMLYLDLDELDRVF
                     AGRWLWSVNRRNLAQFRRSDYLGDPAIPLAEAVRARVREALGRAPTGPIRLLTHLRYF
                     GHCFNPVSFYYCYAEDGTTLDTIVAEITNTPWKERHSYVLPVAGAQRHGAALRWDFAK
                     DFHVSPFVPMQRDYAWRFTVPDDALRVHMDVIDAGKNEFDATLVLERKPLTGPNLARV
                     LLRFPAMTLKIVAAIHWQAFLIFLRRNPVYDHPGKTSQP"
     gene            2326..>2505
                     /locus_tag="VFF05_05300"
     CDS             2326..>2505
                     /locus_tag="VFF05_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZW51252.1"
                     /translation="MNTESTALDAASVPAVYGWLDRTLRARLLRQMSALRDCRLRIVD
                     ALGDITLGTPADDPAQ"
BASE COUNT          431 a          845 c          757 g          472 t
ORIGIN      
        1 tgctgatctg gggatttttc gtctccaccg tcgtgctgtt ccacgccacg gtgacgatca
       61 attccctcgc ccaccgcttc ggctcgcgcc gttttcccac gcgcgacaat tcgcgcaaca
      121 actggctgct tgcgctgctc accttcggcg agggctggca caacaaccat caccactttc
      181 ccggcgccgc gcgccagggc ttccgctggt gggaactgga tgtgacgtat tacctgctgc
      241 gcgggctcgc cgcctgcggg ctggtgtggg acctgaaacc ggtgccgtcc ggccttaaac
      301 gggcgcactg aacgatgcgg atcgcggtcg tgggttcggg tattgcggga cttgccagcg
      361 cgtggctgct atcgcgggag catgaggtga cgctgtacga ggccaacgac tacctgggcg
      421 gccacacgca cacccacgac gttgaaatcg gcggacgcga ctatgccgtg gacaccggct
      481 tcatcgtgtt caatcctccg cattatccac ttttgcacaa attgttcgat gcactcggcg
      541 tcgcttccca accgaccacg atgagttttt cggtcaagga cgagcgctgc ggtctggaat
      601 acaacgccac ggatctgtct ggcctgttct gccagcgccg caatctcgtt tcgccgcgct
      661 tctggcgcat gctcggcgac atccggcgtt tctaccgcga ggcgcccgcg ctgctcgaac
      721 acgatggtgc cggacccgat cttggcgaat acctggccgc caaccgctac tcggatgtct
      781 tccgcgacga tcacctggtg ccgatggcat cggcgctgtg gtcgtcgcca tccgcgctgg
      841 tcttgaaatt cccggtgaag tacctcgccc gattcatggc caaccaccag atgctgcatg
      901 tgcgcggacg cccgcaatgg cgagtagtca agggtggttc gcaggcctat gtgcgcgcgc
      961 tgcgcacggg ctggcgcgta cgcgaacgca ttggcgatgc ggtgaatcga atcacccgcg
     1021 agccgcatca ggtgcgcatc gacagcattt cgggcagcgc gtcttaccac catgcggtgc
     1081 tcgcctgcca cagcgaccag gcccttggct tgctcggcga cgcgaacgaa cgcgaacgtg
     1141 aaattctcgg cgcgatgccg taccaggaaa acgacaccgt gctgcacacc gatgcaagcc
     1201 tgctgccgcg ccggcgccgc gcctgggcgg cgtggaacgc gatcgttcca accgatgcgg
     1261 gcgaacaatg cacggtgagc tactgcatga atctgctgca atcgctcgat tgcccggaac
     1321 ccatcgtggt cacactcaac cgcacggcac agatcgctcc gcagaaaatt ctcaaacgcc
     1381 tgcgctatca ccatccggtc tacacgcagg ccagcgtcgc cgcgcaacaa cgcaaggcgg
     1441 aaatccaggg cgccaaacgc acctggttcg ccggcgccta ctggggctgg ggtttccacg
     1501 aagacggcat gcgcagcgcg gtcgacgtcg caaacggcct gggagtgcac tggccgtgag
     1561 cgcaccgctc gcgagctgcc tctacgaagg ctgggtgcgg catcgccgcc atgcgccgca
     1621 tgcgcacgcg ttccgctacc ggatgtacat gctctacctg gatctggatg aactcgaccg
     1681 tgtcttcgcc gggcgctggc tgtggtccgt gaaccggcgc aatctggccc agttcaggcg
     1741 cagcgattat ctgggcgatc cggctattcc actcgccgaa gcggttcgtg cgcgcgtgcg
     1801 cgaggcgctc ggtcgcgcgc cgaccggccc gatccgcctg ctcacccacc tgcgttactt
     1861 cgggcattgc ttcaacccgg tcagcttcta ctactgctat gccgaggatg gcacgacgct
     1921 ggataccatc gtcgccgaaa tcacgaacac gccatggaag gaacgccaca gctacgtgct
     1981 gccggtcgcc ggcgcacagc gtcacggcgc cgcgctgcga tgggatttcg ccaaggattt
     2041 ccatgtctcg ccgttcgtgc ccatgcagcg cgactacgcc tggcgcttca ctgtgcccga
     2101 cgatgcattg cgcgtgcaca tggacgtgat cgacgcgggc aagaacgaat tcgacgccac
     2161 cctcgttctc gagcgcaagc cgctgactgg gccgaacctg gcccgcgtgc tgctgcgctt
     2221 cccggcgatg accctgaaaa tcgtcgccgc catccactgg caggcgttct tgatctttct
     2281 tcgacgcaat cccgtatacg atcatccggg taaaacgagc cagccatgaa tactgaatcg
     2341 accgccctcg acgctgcttc cgttcccgcc gtttacggat ggctggatcg cacgttgcgt
     2401 gcgcgcctgc tgcggcaaat gtcggcgctg cgcgattgcc gactgcgcat cgtcgatgcg
     2461 ctcggcgata tcacgctggg cacgcctgcg gacgatcccg cacag
//
LOCUS       DASRVO010000424         1272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_194862, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000424 DASRVO010000000
VERSION     DASRVO010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194862"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFF05_05305"
     CDS             <1..307
                     /locus_tag="VFF05_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZW51253.1"
                     /translation="AANGEVVALDPMRAQFLIGRPDPATGTKPEIDLGPFDVQRTLSR
                     RHATLLREGAQYFVREDAATTNGTYLNGERLQTGVAMPVKAGDKLRFGSIEVELIGA"
     gene            321..>1272
                     /locus_tag="VFF05_05310"
     CDS             321..>1272
                     /locus_tag="VFF05_05310"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZW51254.1"
                     /translation="MGTNENEWQGALSAIDLSAIEALKKLKQEHDQLSERLKSMEAMK
                     SNFADAVYVRVRGDYEKRIAALDTEAAPLKQAAREQYAHLRALLGRFEADHEAVKLDQ
                     QELELRHQLGEFDDKEFAKRIKAIENTVAERGQARARGLELKARFLEAFHSESELEQA
                     DAVGTNRFSMADLAAAQASTREVAAIAGGDAPPNRTQTMPALDIPEPPRASMPVPSTP
                     AAGGATQIFRAARLVPQNPEAGKQTYTLTLKPIDIGADAANDVRIGGPGVDPKHAKIV
                     VSMGGFTIVDLGSKHGTRVNAEKVRERPLANEDVIQIGAAR"
BASE COUNT          266 a          421 c          414 g          171 t
ORIGIN      
        1 cgcggccaac ggcgaggtcg tggcgctgga tccgatgcgc gcgcaattcc tgatcggtcg
       61 tccggacccc gcgaccggca cgaaacccga aatcgacctg ggtccgttcg acgtgcagcg
      121 cacgctcagc cgccgccacg cgacgttgct gcgcgaaggc gcgcagtatt tcgtgcgcga
      181 ggatgcggcg acgaccaacg gcacctatct gaacggcgag cgcctgcaaa ccggcgtcgc
      241 catgccggtg aaggccggcg acaaattgcg cttcggttcg atcgaagtcg aactgatcgg
      301 cgcctgacga ggacaacgac atgggcacga acgaaaacga atggcagggc gcgttgtcgg
      361 ccatcgatct gtcggcgatc gaagcgctga agaagctcaa gcaggagcac gaccagctat
      421 ccgagcgcct taaatcgatg gaggcgatga aatcgaactt cgccgacgcg gtatacgtgc
      481 gcgtgcgcgg cgactacgaa aagcgcatcg ctgctttgga taccgaagcc gcaccgctca
      541 agcaggccgc gcgcgaacag tacgcgcacc tgcgcgcctt gctcggccgc ttcgaggcgg
      601 accacgaggc ggtcaagctc gaccagcagg aactcgaatt gcgccaccag ctcggcgaat
      661 tcgacgacaa ggaattcgcc aagcgcatca aggccatcga aaacacggtt gccgagcgtg
      721 gccaggcgcg cgcgcgcgga ttggaattga aggcgcgctt cctcgaagcc ttccacagcg
      781 aaagcgaact tgagcaggcc gatgccgtcg gcaccaatcg cttctcgatg gcggaccttg
      841 ccgccgcaca ggcgagcacc cgcgaagtcg cagcgatcgc gggcggcgac gcaccgccga
      901 accggaccca gaccatgccg gcgctcgata ttcccgagcc gccgcgcgcg agcatgccgg
      961 tgccatcgac accggccgca ggcggcgcga cgcaaatctt ccgcgccgcg cgcctggtgc
     1021 cgcaaaatcc tgaagccggc aagcagacct acacgctcac cctcaagccg atcgacattg
     1081 gcgccgatgc cgccaatgac gtgcgcatcg gcggcccggg cgtggatccc aagcacgcca
     1141 agatcgtggt tagcatgggc ggattcacca tcgtcgatct cggcagcaag catggcacgc
     1201 gcgtgaacgc ggagaaggtg cgcgagcgtc cgctggccaa cgaagacgtg atccagatcg
     1261 gtgcggcgcg gt
//
LOCUS       DASRVO010000425         1301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_141851, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000425 DASRVO010000000
VERSION     DASRVO010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141851"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="VFF05_05315"
     CDS             complement(<1..170)
                     /locus_tag="VFF05_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZW51255.1"
                     /translation="MFLQLDDNGPRYLQLARALKQAILDGRCAPGARLPATRTLAREL
                     EISRNTTLAAYA"
     gene            273..608
                     /locus_tag="VFF05_05320"
     CDS             273..608
                     /locus_tag="VFF05_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HZW51256.1"
                     /translation="MYSVDFIFEPGDYDERFHELNAIIDDAARATPGYLGVDSWRSPD
                     GRLRNAKYYWKTLDALRAFSQHPQHLQAKRDYQKWYRGYHIVIAQVIKSYSDDAIAYF
                     TSNEKDRLT"
     gene            623..1219
                     /locus_tag="VFF05_05325"
     CDS             623..1219
                     /locus_tag="VFF05_05325"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZW51257.1"
                     /translation="MTWPTPVTLRGERVTVEPLRHDHHDGLVESARDGELWNLWYTVV
                     PTPQTMRAEIDRRLALQVAGSMVPFVIVANATQRVVGATSYMNIDAESRRVEIGHTWY
                     AASAQRTGINTETKRLLLAHAFEELDCIAVEFRTHYFNRASRTAIERLGAKLDGILRN
                     HRRGADGSLRDTCVYSIVAGEWPAVRAHLDFELHRARG"
BASE COUNT          272 a          432 c          386 g          211 t
ORIGIN      
        1 aacgcgtagg cggcgagcgt ggtgttgcgc gagatttcca gctcgcgcgc gagcgtgcgc
       61 gtggccggga gtcgcgcacc cggtgcgcaa cgaccatcca ggatcgcctg cttgagagcg
      121 cgcgccagtt gcaggtagcg cggcccgttg tcgtccagct gcaagaacat cgtggcccca
      181 tcaatccgga aaaaaccggt actcgccgga gccaatctga aggtttagat tcggcgcgtc
      241 aagccggcag gccgcgccac atcaggatcc gcatgtacag tgtcgatttc atcttcgagc
      301 cgggcgatta cgacgagcgc ttccacgaac tgaacgcgat catcgacgac gccgcgcgcg
      361 cgacgccggg ttacctcggc gtggattcct ggcgctcgcc ggatggtcgc ctgcgcaacg
      421 ccaagtacta ctggaaaacg ctggacgccc tgcgcgcgtt ctcgcagcat ccacaacatc
      481 tgcaggccaa gcgcgactac caaaaatggt atcgcggcta tcacatcgtc atcgcgcagg
      541 taatcaaatc ctacagcgac gacgcgatcg cgtattttac ctcgaacgaa aaagaccggc
      601 tgacctgaaa aggaatcgat tgatgacttg gccaactccc gtcaccctgc gcggcgaacg
      661 cgtcaccgtc gagccgctgc gccacgacca tcacgatggc ctggtcgaat ccgcgcgcga
      721 cggcgagttg tggaacctgt ggtacacagt cgtgccgacg ccgcagacga tgcgcgcgga
      781 aatcgaccgc cgcctcgcac tgcaagtcgc aggcagcatg gtgccgttcg tcatcgtcgc
      841 caacgcaacg cagcgcgtgg tcggtgcaac gagttacatg aacatcgacg ccgaaagccg
      901 gcgcgtcgag atcggccaca cctggtacgc agccagcgca cagcgcactg gcatcaacac
      961 cgaaaccaag cgcctgctgc tcgcgcatgc gttcgaggaa ctggattgca tcgcggtcga
     1021 attccgcacg cactatttca accgcgccag ccgcacggcg atcgaacgcc tcggcgccaa
     1081 actcgacggc atcctgcgca atcaccgtcg cggcgccgat ggcagccttc gcgacacctg
     1141 cgtgtacagc atcgtcgccg gcgaatggcc ggccgtacgc gcacatctgg atttcgagtt
     1201 gcacagggcg cgcggctgac acggttacac cgcatgccgt agcatccacg aatggattca
     1261 gcacaacacc gcaagacacc gggattgttt tcctgcctgg c
//
LOCUS       DASRVO010000426         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_167139, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000426 DASRVO010000000
VERSION     DASRVO010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167139"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFF05_05330"
     CDS             <1..273
                     /locus_tag="VFF05_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972327.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZW51258.1"
                     /translation="DAALGKLVAAIRTAGGEMLISADHGNLEQMRADDGQPHTQHTVG
                     PVPLVYVGRRATLAPGALRDLAPTVLALMALPQPAQMTGRSLVTFA"
     gene            270..1439
                     /locus_tag="VFF05_05335"
     CDS             270..1439
                     /locus_tag="VFF05_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZW51259.1"
                     /translation="MKRQVTSALLFLGCVCANVYAQADDPQRAAQEAQAKQKLEQVRA
                     QIKALTDAQRDTSAKRGDAVAALREQELKIASTAKELRTLDAQLTTQQGKLDDLLKQR
                     AALDARLKDQRDALAALLRSAYAMGRGEELKLLLAQDNAADIARMLAYYRYFERARLA
                     EIGTLLENLDALAKVQNEIEAQQVMLKTSRDERAAQAQQLDAERLARQMVLEQLDVTL
                     KDQKSRLAALGKDEKALNDLIAKLRDIFADIPKTLVGAESFAVLRGKLPWPLHGRIVE
                     RFGSNTGGGPVSQGVLIAAKGGSEVRAVSHGRVVFADWLRGYGLLMIVDHGDGYLSLY
                     GYHETLLKDVGDWVDAGTVVATSGDSGGRPTPGLYFELRYKGRAVDPAAWLKSSR"
     gene            1490..>2492
                     /locus_tag="VFF05_05340"
     CDS             1490..>2492
                     /locus_tag="VFF05_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZW51260.1"
                     /translation="MLRLAICLLLMLAPLALRADEAKQAPPPNTSASRPGVSMADVRL
                     FTAVFDLIKQAYVDPVDDKTLMQSAIRGMLAGLDPHSEYLDQTGVQALNEDTSGSYDG
                     LGIEVVTLNGALRVIAPIDDTPAARAGIKPGDIIVRIDGMAITSENANQAVDMLRGKA
                     GTKVKLSILHEGASVPEDYALTREAIRVASVRVHELEPSYIYIRVVQFQEDTGNELRS
                     KLGKLLAKEHPLRGAVLDLRSNPGGLLTSAVEISDDFLDSGVVVTTRGRLKQADLSFN
                     ATPGDVLDGAPLVVLVDNGTASAAEIVTGALKDNRRALVIGRRTFGKGSVQTVLPLDD
                     "
BASE COUNT          489 a          794 c          815 g          394 t
ORIGIN      
        1 gatgccgcac ttggcaagct cgtcgccgcg atccgcacgg cgggtggcga gatgttgatc
       61 agtgccgatc acggcaacct cgagcagatg cgcgccgacg acggccagcc gcacacccag
      121 cacacggtcg ggccggtgcc actggtctac gttgggcgcc gcgcgacgct cgcaccgggc
      181 gcattgcgcg atctggcgcc gacggtactc gcgctgatgg ctttgccgca gccggcacag
      241 atgactggtc gttcactggt gacgtttgct tgaagcgcca ggtaacgagc gccttgttgt
      301 ttctcggatg cgtttgcgca aacgtttacg cgcaggccga cgatccacaa cgcgccgcac
      361 aagaagccca ggcgaagcaa aaactcgaac aggtccgcgc tcagatcaag gcgctgaccg
      421 acgcgcaaag ggatacaagc gccaagcgcg gcgatgccgt tgcggcacta cgcgagcagg
      481 aattgaagat cgcttcgacg gcgaaggaac tgcgcacgct cgatgcgcaa ctgacgacgc
      541 aacagggcaa gctcgacgac ttgctcaagc agcgcgcggc gttggatgcc aggctcaagg
      601 atcagcgcga cgcactggcg gcgctgctgc gctcggccta cgcgatgggc cgcggcgagg
      661 aattgaagtt gttgctggcg caggacaacg ccgccgacat cgcacgcatg cttgcctact
      721 accgctattt cgaacgcgcg cggctcgccg aaatcggtac cttgctcgag aatctcgacg
      781 cgctggccaa ggtgcaaaac gaaatcgaag cgcagcaggt gatgttgaag acgtcgcgcg
      841 acgaacgcgc cgcgcaggcg cagcaactcg atgccgaacg cttggcgcgg cagatggtgc
      901 tggaacaact ggacgtcacg ctgaaggatc agaagagtcg actggccgcg cttggcaagg
      961 acgaaaaggc gttgaacgac ctgatcgcga agctacgcga cattttcgcc gacattccga
     1021 aaacgctcgt cggtgcggaa tcctttgcgg tcttgcgcgg caagctgcca tggccgctgc
     1081 acggccgcat cgttgaacga ttcggcagca acaccggcgg cggaccggtc agccagggcg
     1141 tgttgatcgc ggcgaaaggc ggcagcgagg tgcgcgcggt gtcgcatggc cgcgtcgtgt
     1201 tcgccgactg gctgcgcggc tacggcctgc tcatgatcgt ggatcacggc gacggttacc
     1261 tgagcctcta cggctaccac gagacgctgc tcaaggacgt cggcgactgg gtcgacgccg
     1321 gcaccgtcgt tgctaccagc ggcgacagcg gcgggcggcc gacaccgggc ctgtatttcg
     1381 aactgcgcta caagggcagg gcggtggacc ctgcggcttg gctcaaatct tcacgttgat
     1441 cggtctgcgc acgcctataa tgccgatatc gaaatctgcg gtgacgacga tgttgcgact
     1501 tgcgatttgc ctgctgctga tgcttgcgcc tctggcgctg cgcgccgatg aagccaagca
     1561 agcaccgccg cccaatacga gtgcaagcag gccgggcgtt agcatggccg atgtgcgcct
     1621 gttcacggcc gtcttcgatc tgatcaagca ggcctacgtc gatccggtcg acgacaagac
     1681 cttgatgcaa agcgcgatcc gcggcatgct cgccggcttg gatccgcaca gcgaatacct
     1741 cgaccagacc ggcgtgcagg cgctgaacga ggacaccagc ggcagctacg acggtctcgg
     1801 catcgaagtc gtcaccttga acggtgcgct gcgcgtgatc gcgccgatcg acgacacgcc
     1861 ggcggcgcgc gcgggcatca agcccggcga catcatcgtg cgcatcgacg gcatggcgat
     1921 cacctcggaa aacgcgaacc aggccgtgga catgctgcgc ggcaaggccg gcacgaaagt
     1981 gaagctatcc atcctgcacg aaggcgccag cgtgccggaa gactacgcac tcacgcgcga
     2041 ggcgatccgc gtggccagcg tgcgcgtgca cgaactcgaa cccagttaca tctacatccg
     2101 cgtcgtccag ttccaggaag ataccggcaa cgaactgcgc agcaagcttg gcaagctgtt
     2161 ggcgaaggaa catccgctgc gcggcgctgt actcgacctg cgcagcaatc ccggcggctt
     2221 gctcacgtcg gccgtggaga tcagcgatga cttcctggat tctggcgtcg tcgtcaccac
     2281 gcgcggacgc ctgaaacagg cagacctgag tttcaacgcc acgcccggcg acgtactcga
     2341 cggcgcgccg ctcgtggtgc tggtcgacaa cggcactgcc tcggcggccg agatcgtcac
     2401 cggcgcactc aaggacaacc gccgcgccct ggtcattggc cggcgcacct tcggcaaggg
     2461 ctcggtgcag accgtgctgc cgctggacga ca
//
LOCUS       DASRVO010000427         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_12562, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000427 DASRVO010000000
VERSION     DASRVO010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12562"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2763)
                     /locus_tag="VFF05_05345"
     CDS             complement(<1..>2763)
                     /locus_tag="VFF05_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012437704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HZW51261.1"
                     /translation="NIGQAKQDIVAFIESPWDHSKVEPIPALLDEIAGALRILDLNDA
                     AQLMKGIVRFIQVELLRHRRVPTAEQMDKLADALASIEYYLEATHDQRGGRERILDVT
                     RQSLTALGYWPVPPDDGGGDGLPNIPPQPAHAAPAPTSAAAATSATAFTAPSAAPPTS
                     PAAIAMADAHQGDYPRWGEDLPASGEASLKPDVSAAENPAEMPSIELTDESIDEYIAR
                     MDKGEESPAHAEPAPTPEHAIEIEPAVDVAPGRGLEDLVVGELPATESRAHDLAGLRL
                     IETQATATPAPNVRYVEVEEEIEEEIPDTDGVAVTDASFSAVPSDDIDEEIREVFVEE
                     VQDEIDNLGRCLPAWKSDVHDFEKLKPIRRSFHTLKGSGRLVGALALGEFSWKVENML
                     NRVLDKTIPPGPAPQALVETAVGTLPELLRALRGEGNPSADIGQIMAVADRVAAGEEA
                     YVRSGAPQRTKRVKRIVKRLVAVADETPPAAAPAELSATVDIERAFDAAPEEVAALAG
                     GDMTEQAAFAAGPLPNVDPVLFEILKSEVAAHLAAIDGYLSDWRSNPSLPVSEPLLRA
                     VHTLNGAIAMVDIPAITHVLAPLEGYSKRLRAGNFTLDADGAAALGEASALVRDCIAS
                     LETPHPQMPDSGALAAHIAELRDRLPEPEMMHVLFSETHAHEMPPAPELVETEAAAHD
                     DEAALLAELGSLEAEARAEAEMPKAAEVPAEISAVADEDDETARWLREFEQAQAAETH
                     VPVEDAALAAIEPATPAELPETELLSFEDLPAQAELPAPVEPEHAQSEPVAAEPAPIE
                     AVAHEAEHPVEAELPVREAAPHAAVAAMIESAPVLPESSVILPPLLDDPQPDGKLELP
                     DLDEDLLEIFVQEGADILDHSDALMAKLREAPQDRELITGLQRDLHTLKGGARMAGLA
                     PIGDLS"
BASE COUNT          399 a          916 c          938 g          510 t
ORIGIN      
        1 gcgacagatc gccgatcggg gcgaggccgg ccatgcgcgc accgcccttg agcgtgtgca
       61 gatcgcgctg caatccggtg atgagctcgc gatcctgcgg cgcttcgcgc agcttcgcca
      121 tcagcgcatc ggagtgatcc aggatgtcgg cgccttcctg cacgaagatt tcaagcaggt
      181 cctcgtccag gtccggcaat tcgagtttgc cgtccggctg cggatcatcg agcaacggcg
      241 gcaggatgac cgacgactcg ggcagcacgg gtgcagattc gatcattgcg gccaccgctg
      301 cgtgcggcgc cgcttctcga accggcagtt cagcctcgac cggatgttct gcttcgtgcg
      361 ccaccgcctc aatgggcgca ggttccgctg caacaggctc tgactgtgca tgttcgggtt
      421 cgaccggcgc cggaagctcg gcctgcgcgg gcaaatcctc gaacgacagc aattcggttt
      481 ccggcaattc ggcaggcgtc gccggttcga tcgcggcgag agcggcgtcc tccaccggca
      541 catgggtttc ggcggcctga gcctgctcga actcgcgcaa ccagcgcgcg gtttcgtcgt
      601 cctcgtccgc tacggcggaa atctccgcag gcacttcggc ggccttcggc atctccgctt
      661 ctgcgcgcgc ttcggcttcc agcgagccga gttcggcgag caatgcggcc tcgtcgtcgt
      721 gtgccgcagc ttcggtttcg accagctccg gtgcgggcgg catttcgtgc gcatgcgttt
      781 cggaaaacag cacgtgcatc atttccggtt ccggcaaacg atcgcgcagc tccgcgatgt
      841 gcgcggcgag cgcgccggaa tccggcatct gcggatgcgg cgtctccagc gaggcgatgc
      901 aatcacgcac cagcgcggac gcttcgccca gtgccgcggc gccatcggca tcgagcgtga
      961 aattgcccgc gcgcaagcgc ttggaatagc cttccagcgg cgccagcaca tgcgtgatcg
     1021 cgggaatgtc gaccatcgcg atcgcgccat tgagcgtgtg cacggcgcgc aacaacggtt
     1081 cgctgaccgg caacgacggg ttggaacgcc agtcggaaag atagccgtcg atcgcggcca
     1141 ggtgcgcggc gacttcgctc ttgagtatct cgaacaggac cggatccacg ttcggcagcg
     1201 ggccggcggc gaacgccgcc tgctcggtca tgtcgccacc ggccaatgcg gcgacttctt
     1261 ccggcgccgc gtcgaaggcg cgctcgatgt cgaccgtggc gctcaattcg gccggagcgg
     1321 cggccggcgg ggtttcgtcc gccaccgcga ccaggcgctt gacgatgcgc ttgacgcgct
     1381 tggtacgctg cggcgcaccg ctgcgcacat aggcttcctc gcccgcggcg acgcggtcgg
     1441 caacggccat gatctggccg atgtcggcgc tgggattgcc ttcgccgcgc agcgcgcgca
     1501 acaattcggg cagcgtgccg acggccgttt ccaccagcgc ctgcggggcc ggtccgggcg
     1561 ggatggtctt gtcgagcacg cggttgagca tgttctcgac tttccagctg aactcgccca
     1621 gcgccagcgc accgaccagt cggcccgaac ccttgagcgt gtggaacgag cgccggatcg
     1681 gcttgagttt ctcgaaatcg tgtacgtcgg acttccacgc cggcaggcag cggccgagat
     1741 tgtcgatttc gtcctgtact tcctcgacga agacttcgcg gatttcctcg tcgatgtcgt
     1801 cggacggcac cgcgctgaag ctggcatcgg tcaccgcgac gccatcggta tccggaattt
     1861 cttcctcgat ttcttcctcg acctcgacat agcgcacgtt cggcgccggc gtcgccgtgg
     1921 cctgcgtttc gatcaggcgc aaaccggcca ggtcgtgcgc gcgcgattcg gtggccggca
     1981 gttcgcccac gaccaggtct tccaggcccc tgcccggcgc cacgtcgacg gccggttcga
     2041 tttcaatcgc atgttcaggc gtcggcgcgg gttcggcgtg cgcgggcgat tcctcgccct
     2101 tgtccatgcg cgcgatgtat tcgtcgatgc tctcgtcggt cagctcgatg ctcggcatct
     2161 ccgccggatt ctcggcagcg ctgacatccg gcttgagact ggcctcgccg ctggcgggaa
     2221 ggtcctcgcc ccagcgcgga tagtcgccct ggtgcgcgtc agccatcgcg atcgcggccg
     2281 gagacgtggg cggtgcggcg gatggagcgg tgaatgccgt ggcgctggtg gcggctgccg
     2341 cagatgtcgg cgcaggcgca gcatgtgcgg gttgcggcgg tatgttcggc agcccgtcgc
     2401 cgccgccgtc atcgggcggc accggccagt aaccaagcgc ggtcaggctc tgccgggtca
     2461 cgtcgaggat gcgctcgcgg ccgccgcgtt ggtcgtgcgt ggcttccagg taatactcga
     2521 tcgatgccag cgcatcggcg agcttgtcca tctgctcggc ggtcggcacg cggcggtggc
     2581 gcagcagctc gacctggatg aagcgcacga tgcccttcat cagttgcgcg gcgtcgttca
     2641 ggtcgaggat gcgcaaggcg ccggcgatct cgtcgagcaa cgccggaatc ggttcgacct
     2701 tggagtgatc ccacggcgat tcgatgaaag cgacgatgtc ctgcttggcc tggccgatgt
     2761 tgg
//
LOCUS       DASRVO010000428         1291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_12642, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000428 DASRVO010000000
VERSION     DASRVO010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12642"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFF05_05350"
     CDS             <1..276
                     /locus_tag="VFF05_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZW51262.1"
                     /translation="LDAKVTLAIDDLYKIPDDSAAAIFTSGLLGDQYVALQPGGSPDN
                     FKNGDEVVLTQSSMQLEELIGKYLVGGDAGKSGDKSKDAAAPGDKKE"
     gene            286..918
                     /locus_tag="VFF05_05355"
     CDS             286..918
                     /locus_tag="VFF05_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZW51263.1"
                     /translation="MRVAYVLLCIFGLTAFAPAHADTPTPNQVVQGIVDDLGKTMDAH
                     RGDLRNNRDELLKTIDAIVLPHFDIDYASILVLGQNARSATPAQRARFAKAMYNSITH
                     RYAEGLLKYTEGRVKVLPFQGQLNDKRTLVRTQVVLDDGKIVPVDYAFRKARDGSWKA
                     YDVIIEGISYVTNYRNQVAAEIAKSSLDALITRLESQGADALKSMEKEGQ"
     gene            915..1217
                     /locus_tag="VFF05_05360"
     CDS             915..1217
                     /locus_tag="VFF05_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZW51264.1"
                     /translation="MSAPFFGIEADTLGGLRAAGAITFANARLALAQLPPPAHGADLN
                     VDLGALDGGDSATLAVLIAWSAAARRAGAALHYLRVPQGLRNLAKLCEVDGLLGFA"
BASE COUNT          241 a          413 c          414 g          223 t
ORIGIN      
        1 ctcgacgcca aggtgacgct ggccatcgac gacctctaca agattcccga cgactcggcc
       61 gcagccatat tcaccagcgg tttattgggc gaccagtacg ttgccttgca gccgggcggt
      121 tcgccggaca atttcaagaa cggcgacgag gtcgtgctga cgcaaagctc catgcagctc
      181 gaggaactga tcggcaagta cctggtcgga ggcgatgccg gcaagtccgg cgacaagtcc
      241 aaagatgccg ctgcgcccgg cgacaagaag gaataggagt ccgccatgag agtcgcatat
      301 gtgttgctat gtattttcgg tttgacggcg ttcgcgcctg cgcatgccga cacgcccacg
      361 cccaaccagg tggtgcaggg tatcgtcgac gaccttggca agaccatgga cgcgcaccgc
      421 ggggacttgc ggaacaatcg cgacgaactg ctcaagacca ttgatgcgat cgtgctgccg
      481 cacttcgaca tcgactacgc ctcgatcctg gtgctcggcc agaacgcgcg cagcgccaca
      541 ccggcacagc gcgcgcgctt cgccaaggcg atgtacaact cgatcacgca tcgctacgcc
      601 gaaggcctgc tcaagtacac ggaagggcgg gtcaaggtgc tgccgttcca gggccagttg
      661 aatgacaagc gcacgctggt gcgcacgcag gtcgtgctcg acgacggcaa gatcgtgccg
      721 gtcgactacg cgttccgcaa ggcgcgcgac ggctcgtgga aggcctacga cgtcatcatc
      781 gagggcattt cctacgtgac caactaccga aatcaggtcg ccgcggaaat cgccaagtcc
      841 agcctcgatg ccctgatcac gcggctcgaa tcgcagggcg ccgatgcgct caagagcatg
      901 gagaaggaag gccagtgagc gcgccgttct tcggcatcga agcggacacg ctcggcggcc
      961 tgcgcgcggc cggcgcgatc acgttcgcga acgcacgcct tgcgctggcg caattaccgc
     1021 cgcccgcgca tggcgctgac ttgaacgtgg atctgggtgc cctcgacggc ggcgacagcg
     1081 cgaccctggc cgtgctcatc gcctggagcg cggcggcgcg ccgcgcgggt gccgcgcttc
     1141 attacctgcg cgtgccacaa ggcctgcgca acctcgcaaa gctgtgcgaa gtggatggac
     1201 tgctcggatt cgcctgactg cgtcgcggct tagtgttgtg gcggggttcc gccgcttttg
     1261 tcgggccgct tctgctgctt ttcgtactgc t
//
LOCUS       DASRVO010000429         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_246209, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000429 DASRVO010000000
VERSION     DASRVO010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246209"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFF05_05365"
     CDS             complement(<1..491)
                     /locus_tag="VFF05_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HZW51265.1"
                     /translation="MFDKILIANRGEIACRVIKTARKMGIRTVAVYSDADRDARHVEL
                     ADEAVHIGAAPSRESYLQGDRIIAACKQTGAQAVHPGYGFLSENEAFARRVEEEGITF
                     IGPKHHSIAAMGDKIASKKLAKDARVNTIPGYNEAIGSAEQAVEIAKGIGYPVMIKAS
                     AGGG"
     gene            complement(740..>1152)
                     /locus_tag="VFF05_05370"
     CDS             complement(740..>1152)
                     /locus_tag="VFF05_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZW51266.1"
                     /translation="IIKHGAKLLFAFAECTVPKITVITRKAYGGAYDVMSSKHLRGDV
                     NFAWPNAEIAVMGAKGAVEIIFREDKNDPAKLAAREAEYKARFANPFVAGARGFIDDV
                     ILPHETRKRICRSLIMLAGKKLENPWRKHGNIPL"
BASE COUNT          174 a          386 c          349 g          243 t
ORIGIN      
        1 ccgccgccgg ccgaggcctt gatcatcacg gggtagccga tccctttcgc gatctccacc
       61 gcctgctcgg cgctgccgat ggcttcgttg tagccgggga tcgtgttgac ccgggcgtcc
      121 ttggcgagct tcttcgatgc gatcttgtcg cccatcgccg cgatcgaatg gtgcttgggg
      181 ccgatgaacg tgatgccctc ttcctccacc cggcgcgcga acgcctcgtt ttcggagagg
      241 aagccgtagc cgggatgcac ggcctgcgcg ccggtctgct tgcaagccgc gatgatccgg
      301 tccccctgca ggtacgactc gcgcgatggc gccgcgccga tgtgcaccgc ttcgtcggcc
      361 agctcgacat gccgcgcgtc gcggtccgca tcggaataca ccgcgaccgt cctgatcccc
      421 atcttcctgg ccgtcttgat gacgcggcag gcgatttcac cgcgattcgc gatcaatatc
      481 ttgtcaaaca tggcccggct ccatcgcgat gaaatcgcct gcgcacgctg cgcgtgccac
      541 gccgtctggt gtgcgcatca catgcgcttc gcccaaatgt gagcgcaccc ccgcgccttc
      601 ggcgcgagca ccgcgattgg ctatcagtat cttttcaaac acgagagtct ccgtttcttg
      661 tcattgcggg ctcgacccgc aatccatgcg gcgttcagcc atacgtgatg tggatgccgg
      721 atcaagtccg gcatgacagt cagagaggga tgtttccatg tttgcgccac gggttctcca
      781 gtttcttgcc cgccagcatg atgagcgaac ggcagatgcg cttgcgcgtt tcgtgcggca
      841 ggatcacgtc gtcgatgaag ccccgcgcgc ccgcgacgaa cggattggcg aagcgcgcct
      901 tgtactcggc ctcgcgcgcc gccagtttgg ccgggtcgtt cttgtcctcg cgaaagatga
      961 tctccaccgc gcccttcgca cccatcacgg cgatctcggc gttcggccac gcgaaattga
     1021 cgtcgccgcg caggtgtttc gagctcatca cgtcgtaggc cccgccgtat gccttgcggg
     1081 tgatcaccgt gatcttgggc acggtgcatt cggcgaacgc gaacagcagt ttggcgccgt
     1141 gcttgatgat gc
//
LOCUS       DASRVO010000430         1629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_64578, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000430 DASRVO010000000
VERSION     DASRVO010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64578"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /gene="rimM"
                     /locus_tag="VFF05_05375"
     CDS             complement(<1..403)
                     /gene="rimM"
                     /locus_tag="VFF05_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462794.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZW51267.1"
                     /translation="MTVGRIVGIHGVDGWVKLESWTEPRLRIFSYRPWRLTLAGSEFE
                     AASAQGHEQGKGMVAKLPGCDDRVAAAKLVGATIQVPRSALPKPKRGEYYWTDLEGLI
                     VVTVDGVDLGKVSHLFATGANDVLVVRGERER"
     gene            complement(432..689)
                     /gene="rpsP"
                     /locus_tag="VFF05_05380"
     CDS             complement(432..689)
                     /gene="rpsP"
                     /locus_tag="VFF05_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZW51268.1"
                     /translation="MVKIRLSRGGAKGRPFYHIVVADERKARDGRNIERVGFYNPVAS
                     GKDVRLQLDTDKVNAWVAKGAQMSDKVRWLMKEAGKQAKAA"
     gene            complement(852..>1629)
                     /locus_tag="VFF05_05385"
     CDS             complement(852..>1629)
                     /locus_tag="VFF05_05385"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HZW51269.1"
                     /translation="WAIGTTRRYTVGVWIGRPDGTPLPGQYGAVTALPLMFEAIDGLP
                     RNVLAATPEAPPASVRQADVCWPLGLAFDPKHPDSCQQKHTAWILDGVIPPTLPERDM
                     QVWSAGLVQVRVDMHSGRRLSPGCEAANAKVVEIARWPALAYPWLAPDLRRVSSVPPL
                     QAGCAADGLDAAQPIRIDGVANGAAIARAPNSDKPAALRLRVLGAERERILWLVNGKL
                     EGETRAAQPFEHAFADTGEQTITALAASGAYAQLQIRVLR"
BASE COUNT          275 a          567 c          514 g          273 t
ORIGIN      
        1 ggcgttcgcg ctcgccacgc acaaccagca cgtcgttcgc gccggtcgcg aacagatggc
       61 tgaccttgcc cagatccaca ccatccacgg tgaccacgat gagaccttcc agatccgtcc
      121 agtagtattc gccgcgcttg ggcttgggca gcgcggatcg cggaacctgg atcgtcgcgc
      181 cgaccagctt cgccgccgca acgcgatcgt cgcagccagg caatttggcg accatgccct
      241 tgccctgctc gtgaccttgc gcggaagcgg cttcgaattc cgaacccgcc agggtcagtc
      301 gccacggccg gtaggaaaaa atccgcaggc gcggctcggt ccaggattcc agcttgaccc
      361 aaccgtcgac gccatggatt ccaacgatcc tgccgaccgt caaaagtcga tcggggtaca
      421 cgtgcgccgg atcaggccgc cttggcctgc ttgccggctt ccttcatcag ccagcgcacc
      481 ttgtcggaca tttgcgcgcc cttggcgacc caggcgttga ccttgtcggt gtcgagctgc
      541 aggcgcacgt ccttgcccga ggcgaccggg ttgtagaaac ccacgcgctc gatgttgcgg
      601 ccgtcgcgcg ccttgcgctc gtcggcaacg acgatgtgat agaacggacg gcctttggcg
      661 ccgccgcggg agagacgaat cttgaccatg tttatttctc gagaatttat cgggtaagaa
      721 ttcacacggg ccgacaggac acgcacacag acagcttgcg cgcagcaagg caatccaggc
      781 cagtgagccg cgtattttag cctgaatcgg gaaaaatgaa agcccccgca aggacttggg
      841 cccagggaac ctcagcgcaa tacccgaatc tgcaactggg catacgcccc gctcgccgcc
      901 agcgccgtga tggtctgctc gccggtgtcg gcgaaggcgt gctcgaaggg ttgggcggcg
      961 cgggtttcgc cttcgagttt gccattgacc agccacagga tgcgctcgcg ttcggcgccc
     1021 agcacccgca agcgcagcgc cgccggtttg tcgctgttcg gcgcccgcgc gatcgcagcg
     1081 ccgttggcga cgccgtcgat gcggataggt tgcgccgcat ccagaccgtc ggctgcgcat
     1141 ccggcttgca gcggcggtac gctgctgacg cggcgcaggt ccggcgccag ccacggatac
     1201 gccagagccg gccagcgcgc aatttccacc accttcgcgt tcgccgcctc gcagccggga
     1261 ctcaggcgcc ggccgctgtg catgtccacc cgcacctgca ccaggcccgc gctccaaacc
     1321 tgcatgtcgc gctcgggcag cgttggcggg atcacgccat cgagaatcca cgccgtgtgt
     1381 ttttgctggc agctatccgg atgcttcgga tcgaacgcga gtcccagcgg ccagcacaca
     1441 tccgcctggc gcacgctcgc cggcggcgct tcgggcgtgg ccgccagcac gttgcgcggc
     1501 aagccgtcga tggcctcgaa catcagcggt agcgcggtga cggcgccgta ctggcccggc
     1561 agcggcgtgc catccggacg gccaatccac acgccgacag tgtagcgcct cgtcgtgccg
     1621 atggcccag
//
LOCUS       DASRVO010000431         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_194923, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000431 DASRVO010000000
VERSION     DASRVO010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194923"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFF05_05390"
     CDS             <1..529
                     /locus_tag="VFF05_05390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW51270.1"
                     /translation="IPDVRLVVLGDETPDLLHESGAGLAERIRRRATELGIADTVRFI
                     GPQDDATLADAWRAADAHVFPVREVAGDIEGFGMVAVEAAAHGLPTVAFAVGGVPDAV
                     CDGVSGYLVPPGDHAQFAAHCIELLRARTSAPLRASARRFAEGFAWKTFGVQLRAQIR
                     GLAADTALASGEFKP"
     gene            615..>1530
                     /locus_tag="VFF05_05395"
     CDS             615..>1530
                     /locus_tag="VFF05_05395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZW51271.1"
                     /translation="MSADNEPLETSDPDRLVAEPLVSVLMITYKHAAFIGQAIEGVVA
                     QQCDFPFELIIGEDASQDATLAIALDYQRRFPHIVRVIHARANVGMNANGSRIFARAR
                     GEFVAFCEGDDYWCSPHKLARQVDAIRSDARVGIVHADWVRSRCRNGIWTHNYRKSVH
                     AHVPLRLLQGDLFATWHFPKILRTCTILLRRSTVIEMAASGLARREYKFGDSVLSAYV
                     TSKWRVAYVPEVVAVYHESPNSALRSGAGSRVGLYKSALEFDDDARAYFAGRAAYGDG
                     YRWEISVALLLWSMRARDGRSAWAALRDL"
BASE COUNT          244 a          477 c          509 g          300 t
ORIGIN      
        1 gattcccgac gtccgccttg tagtgctcgg cgacgagacg cccgatctcc tgcacgaaag
       61 cggtgccggg ctggccgagc gcattcgtcg gcgcgccact gaactcggca tcgcggacac
      121 ggtgcgcttc atcggcccgc aggacgacgc cacacttgcc gacgcctggc gtgcggccga
      181 tgcgcacgtc tttccggtgc gtgaagttgc cggtgacatc gaaggtttcg gcatggtcgc
      241 cgtcgaggcg gccgcgcacg ggctgccaac cgtcgccttt gccgtcggcg gagtgccgga
      301 tgcggtgtgc gatggagttt ccggttacct cgtgccgccc ggggaccatg cgcaattcgc
      361 ggctcattgc atcgaactgt tgcgcgcacg gacgagcgca ccgttgcgcg ccagtgcgcg
      421 tcggttcgcg gaaggcttcg cgtggaagac cttcggcgtg cagttgcgcg cgcagatacg
      481 cggccttgcc gcggacaccg cgctggcgtc cggcgagttc aagccatgac gcgccggttg
      541 gcgcgccgtg ggccatgtct ggcacccgtg gcgttccgcc gtatcatcgc gataattcga
      601 gcgcggactc tttcgtgagc gcagacaacg aaccgttgga aacgagcgat ccggatcggc
      661 tggtcgctga gccgctggtc agcgtgctca tgatcactta caagcatgcc gctttcatcg
      721 gacaggcgat tgaaggtgtg gtcgcacagc aatgcgactt tccgttcgag ctgatcatcg
      781 gcgaggacgc ctcgcaggat gccacgctcg caatcgccct ggattaccag cgccggtttc
      841 cgcacatcgt gcgcgtgatt catgcgcgcg ccaacgtcgg catgaatgcc aatggcagcc
      901 gcatctttgc gcgtgcgcgc ggcgaattcg tcgcgttctg cgaaggcgac gattactggt
      961 gctcgccgca caagctcgca cgccaggtag atgccatccg ctccgacgcg cgtgttggca
     1021 tcgtgcacgc cgactgggtc aggtccagat gcaggaacgg catctggaca cacaattatc
     1081 gaaagtcggt gcatgcgcac gtacccctgc ggctgttgca gggcgacttg ttcgcgacct
     1141 ggcatttccc gaagatcctg cgtacctgca cgattctgtt gcgacggagt accgtgatcg
     1201 agatggctgc ttcaggtctg gcacggcgtg aatacaagtt cggtgacagc gtgctgagcg
     1261 cgtacgtcac gtccaaatgg cgtgtggctt acgttccgga agtcgtcgcc gtgtatcacg
     1321 aaagtccgaa ttccgcgttg cgctcaggcg ccggttcgcg cgtcggcttg tacaagtcgg
     1381 cactcgaatt cgacgacgac gcacgcgcct acttcgcggg acgcgccgct tatggagacg
     1441 gctatcgctg ggaaatcagc gtcgccttgc tgctgtggtc gatgcgggcg cgggacgggc
     1501 gctcggcctg ggccgcgttg cgcgatctct
//
LOCUS       DASRVO010000432         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_272761, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000432 DASRVO010000000
VERSION     DASRVO010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272761"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..463
                     /gene="gspI"
                     /locus_tag="VFF05_05400"
     CDS             26..463
                     /gene="gspI"
                     /locus_tag="VFF05_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HZW51272.1"
                     /translation="MRAERRLFPPRSNPLPRHRRWRGSVSGFTLLEVLVALLVLALAL
                     LALSRTAANQVDAFGAMRERTIAGWLAADVLAQTRLATPFPATGTSDGRRRFGGRDWR
                     YDVVVQGTPVASIRRIDVHVYAPDDPKSPIATLTGFGGLDLQP"
     gene            460..>1000
                     /gene="gspJ"
                     /locus_tag="VFF05_05405"
     CDS             460..>1000
                     /gene="gspJ"
                     /locus_tag="VFF05_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HZW51273.1"
                     /translation="MKRGFTLIEVLVATAVFSIMAALAYGGLNSIARTRGDIAGQEAD
                     FAALMRAVGSLDRDLNSVVARPVRGAAGQVLPVLVGASDHIELTRLGFANPQAEPRSN
                     LQRVFYELDGSKFERGRYAVLDRAANSTPQVDDLHLKAEDFRLRYFDGASSQWLDAWP
                     PPQSKPEALPRAVQWRLRTP"
BASE COUNT          149 a          333 c          328 g          190 t
ORIGIN      
        1 ctgaagattg cagaggtgaa tattcgtgcg cgctgagcgg cgtttatttc cccctcgctc
       61 caaccctctc ccccgacacc gtcggtggag agggagtgtt tccggtttca ccttgctcga
      121 agtgctggtc gcattgctcg tgctcgcgct tgcattgctc gcgctgtcgc gcacggcggc
      181 aaaccaggtc gatgccttcg gcgcgatgcg cgagcgcacc attgccggct ggctggcggc
      241 cgacgtactc gcgcagacgc gcctggcgac gccgtttccg gccacgggca cgagcgacgg
      301 ccgccgccgc ttcggtggac gcgactggcg ttacgacgtc gtcgtgcaag gtacgccggt
      361 ggcgtcgatc cggcgcatcg acgtgcatgt ttatgcgccg gacgatccga agtctccgat
      421 cgcgacgctg acgggattcg gcggtctgga tttgcagcca tgaaacgcgg attcaccttg
      481 atcgaggtgc tggtcgccac ggccgtgttc tcgatcatgg ccgcgcttgc ctatggcgga
      541 ttgaattcca tcgcgcgcac gcgcggcgac attgccgggc aggaagccga tttcgccgct
      601 ctcatgcgcg cggtcggttc gctggatcgc gatctgaaca gcgttgtcgc tcgccctgtg
      661 cgcggcgcgg cagggcaggt gctgccggtg ctggtcggcg catccgacca catcgaactc
      721 acgcgcctgg gattcgccaa tccgcaggcc gagccgcgct cgaacctgca acgcgtgttc
      781 tacgaactcg acggcagcaa attcgaacgc ggccgttatg ccgtgctcga ccgcgccgcg
      841 aacagcacgc cgcaggtcga cgacctgcac ttgaaggccg aggatttccg cctgcgctat
      901 ttcgacggtg cgtcctctca gtggctggat gcctggccgc cgccgcaatc caaacccgag
      961 gcgttgccgc gcgcggtgca atggcgcctg cgcacgccaa
//
LOCUS       DASRVO010000433         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_12788, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000433 DASRVO010000000
VERSION     DASRVO010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12788"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1054
                     /locus_tag="VFF05_05410"
     CDS             <1..>1054
                     /locus_tag="VFF05_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZW51274.1"
                     /translation="IDARWPGERAVVEALAQFDALQRQDVPQERRCSYYCDPQTLAPL
                     RDRIEAIAAELGCSVLYSADRYLDILPPATNKGSTLGKLAARLRLAPGRVLAAGDTLN
                     DLSMFQAGFPGVCVGDSEAALLETTRDLPDTLHAKAPGCGGILEAIDHFDLLAADDPL
                     RQPPAAGREGKADLVMVYHRLPYEEAIENGIRVQRAPRSPNGIIPSLLGFFSGGRNGS
                     WLAWSIRDGHDESFATHTPVDAKRYSGLTAVRVPLSHSEVDTFYKRFSKEAFWPMLHT
                     FWERARFCEDDWQVFVRVNRKFAEAAAAEAAPGATVWIHDYNLWMMPGRLRELRPDLT
                     IAFFHHTYFPSADVFNV"
BASE COUNT          178 a          355 c          336 g          185 t
ORIGIN      
        1 atcgacgcac gctggccggg cgagcgcgcc gtggtcgaag ccctggcgca attcgatgcg
       61 ctgcagcggc aagacgtgcc gcaggaacgg cgttgctcgt attactgcga cccacagacg
      121 ctggcgccgt tgcgcgatcg catcgaggca attgccgctg aactgggctg ctcggtgctg
      181 tattcggcgg atcgctatct ggacatcctg ccgccggcca cgaacaaggg cagcacgctg
      241 ggcaagttgg ccgcgcgcct gcgtctcgcg ccgggccggg tactggcggc cggcgacacg
      301 ctcaacgatc tgtccatgtt tcaggccggc ttccccggtg tgtgcgtggg cgattcggaa
      361 gccgcgctgc tggagacgac gcgcgacctg cccgacacgc tacacgccaa ggcgccgggt
      421 tgcggcggca tcctcgaggc aatcgatcat ttcgaccttc tggccgccga cgatccactg
      481 cgccaaccac cggccgccgg gcgcgaaggc aaggcggacc tggtcatggt ctaccatcgc
      541 ctgccctacg aggaagcgat cgaaaacggc attcgcgtgc agcgcgctcc gcgctcgccg
      601 aacggcatca tccccagcct cctcggattc ttttccggcg ggcgcaacgg atcctggttg
      661 gcatggagca tccgcgacgg gcacgacgaa tccttcgcca cgcacacgcc ggtggatgca
      721 aagcgctatt cgggactgac tgccgtacgc gtgccgctca gccacagcga agtcgacacg
      781 ttctacaagc gtttttccaa ggaagcgttc tggccgatgc tgcacacgtt ttgggaacgt
      841 gcgcgcttct gcgaagacga ttggcaggtg ttcgtgcgcg tgaaccgcaa gttcgccgag
      901 gccgccgccg ccgaagcggc acccggcgcc accgtgtgga tacacgacta caacctgtgg
      961 atgatgccgg gccgcctgcg cgagctgcgt ccggatctga ccatcgcatt tttccatcac
     1021 acctattttc cctccgccga cgtgttcaac gtgg
//
LOCUS       DASRVO010000434         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_246316, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000434 DASRVO010000000
VERSION     DASRVO010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246316"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFF05_05415"
     CDS             <1..283
                     /locus_tag="VFF05_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW51275.1"
                     /translation="MAQWNASYPERIYTQSYEALVADPETQIRELLDFCGLPFDAACL
                     RPHEVERSIRTASASQVREPIRRDTARTGKYGALLDPLRKELGLPPFVA"
     gene            306..1568
                     /locus_tag="VFF05_05420"
     CDS             306..1568
                     /locus_tag="VFF05_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavohemoglobin expression-modulating QEGLA
                     motif protein"
                     /protein_id="HZW51276.1"
                     /translation="MADSPASLERIAELDRRLVAAVKGIKLLAALSWPASAQEAFLAG
                     WHAKNPRLPDVDYAKSDLARTREELEAIFLAADAGHPLGDYLRRSAQSWRTATELLDA
                     AGTSAIAEHSIHLFGRPNDRLPGGTVSTLDAARHFIELADELGGEISAHEADYCIPAD
                     LLQHELRQRLDAFFGNGVVRVETDPNLIAKAAAGATRIRLRSATCFSEYDKNQLLEHE
                     AFVHSLTALNGRAQPSLGSLATNSPRITATQEGLATFAELMSGSIDIGRMKRISLRIL
                     AIDNALAGADFIDVFKFFLDSGQTDADSFASAARVFRGAPLTGGCAFTKDAVYLHGLL
                     SVHTFFRWALKNKKLQWCRNLFAGKMALHDVIALAPWFESGYIAAPVYLPPWMQRSNG
                     LAGALAFSLFANRIRLDKVETEDLVLGV"
     gene            1621..2493
                     /locus_tag="VFF05_05425"
                     /pseudo
     CDS             1621..2493
                     /locus_tag="VFF05_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
BASE COUNT          465 a          905 c          845 g          441 t
ORIGIN      
        1 gatggcgcaa tggaacgcgt cgtatccgga gcggatctac acgcagtcct acgaggcgct
       61 cgtggccgat ccggaaacgc agatccgcga gctgctggat ttctgcggct tgccgtttga
      121 tgcggcctgc ctgcgcccgc acgaggtcga gcgcagcatc cgcaccgcca gcgcctcgca
      181 ggtgcgcgaa ccgatccgcc gcgacaccgc gcgcaccggc aagtatggcg cgctgctcga
      241 tccgctgcgc aaggaactcg gattgcctcc cttcgttgcc tagcgcgctg cgctatagtt
      301 cgcgcatggc cgattcgccg gcatcgctcg aacgcatcgc ggaactggac cgtcgcctcg
      361 tcgcggcggt caagggcatc aagctgctcg ccgcgctgag ctggccggcc agcgcgcagg
      421 aagccttcct cgccggctgg cacgcgaaga atccacgatt gcccgacgtg gattatgcga
      481 aatccgattt ggcgcgcacg cgcgaagagc tcgaggcgat cttcctcgcc gcggacgcgg
      541 gacacccgct cggcgactac ctgcgccgca gcgcgcaatc ctggcgtact gccaccgaac
      601 tgctcgacgc ggccggcacg agtgccatcg ccgaacattc gatccatctg ttcggccgcc
      661 cgaacgaccg cctgcccggc ggtacggtca gcacgctgga cgccgcgcgg catttcatcg
      721 agctggccga cgaactcggc ggcgagattt ccgcgcacga ggccgattac tgcattccag
      781 ccgatctgct gcaacacgaa ttgcggcagc gcctggatgc gttcttcggc aacggcgtgg
      841 tgcgcgtgga gaccgatccg aacctgatcg ccaaggccgc cgccggcgcc acgcgtatcc
      901 gcctgcgttc ggcgacgtgt ttttcggaat acgacaagaa ccagctgctc gagcacgagg
      961 ccttcgtgca ttcgctgacc gcgctcaacg gccgcgcgca gcccagtctc ggctcgctgg
     1021 cgacgaattc gccgcgtatc accgccacgc aggaaggcct ggccacgttc gccgagctca
     1081 tgtccggatc catcgacatc ggccgcatga agcgcatttc cctgcgcatc ctcgccatcg
     1141 acaatgcgct ggccggtgcg gatttcatcg acgtgttcaa gtttttcctc gattcgggcc
     1201 agaccgacgc cgacagcttc gcctcggccg cgcgcgtgtt ccgtggcgca ccgttgaccg
     1261 gcggctgcgc gttcaccaag gatgcggtgt atttgcacgg cttgctgtcc gtgcacacgt
     1321 tcttccgctg ggcgttgaag aacaagaagc tgcaatggtg ccgcaacctg ttcgccggca
     1381 agatggcgct gcacgacgtg atcgcgctgg cgccgtggtt cgagtccggc tacatcgcgg
     1441 cgcccgtgta tctgccgccg tggatgcagc gatcaaacgg cctggccggc gcgctcgcgt
     1501 tttcactgtt cgcgaatcgt atccggctgg acaaggtcga aaccgaagac ctcgtgctcg
     1561 gcgtctgact gccacggtat caagtcatcg cgctgtgcta agctcgcaaa cccgcattgg
     1621 atgcgggatt gcgagcgtta cgccatgtcc accaccgacg acctgaagca agccgccctc
     1681 gactaccatc gccgtaaccc gccgggcaag atccgcatat cgtcgaccaa gccgctagtg
     1741 acgcaacgcg acctggcgct cgcgtattcg ccgggcgtgg cggcggcctg cgacgcgatc
     1801 gtcgccgatc cgggcgaagt ctccacggtc accgcgcgcg gcaacctcgt cgccgtgatc
     1861 acgaacggca cggcagtgct gggcctgggc aacatcggcc cgctcgcggc gaaaccggtg
     1921 atggaaggca agggcgtgct gttccagaaa ttcgccggca tcgacgtgtt cgacatcgaa
     1981 gtcgccgagc gcgacccgga caagctcgtc gacatcatcg ccgcgctgga accgacgttc
     2041 ggcggcatca acctcgagga catcaaggcg ccggagtgct tcgaagtcga gcgcaagctg
     2101 cgcgaacggg tgaagatccc ggtgttccac gatgaccagc acggcacctc gatcatcgtc
     2161 ggcgccgccg tcatcaatgc gttggccgtg gtcggcaagg acatcggcgc ggtcaagctg
     2221 gtctcgaccg gcgccggcgc ggccggcatc gcctgcctgg acatgctcgt gcagcttggc
     2281 gtgaagccgg agaacatcct cgttgtcgac cgcgtcggcg tgctctacaa agggcgcgcg
     2341 gacgacatgg atccggccaa gcagcgctac gcgcgcgaga cgaaggcgcg cacgctggcc
     2401 gacatcgtca agggcgcgga catcttcctg ggactgtccg ccgccggcgt actcagcggc
     2461 gacatggtca agaccatggc gccgcgaccg atcccgacga gatcgtcacg atcgtgaagg
     2521 cgctcgagcc gaccttcggc ggcatcaacc tcgaggacat caaggcgccc gagtgcttcg
     2581 agatcgagga gcgcctgcgc aaggagatgc agattcccgt cttccatgac gaccagcacg
     2641 gaacggcgat catctc
//
LOCUS       DASRVO010000435         1202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_324663, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000435 DASRVO010000000
VERSION     DASRVO010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324663"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFF05_05430"
     CDS             complement(<1..1011)
                     /locus_tag="VFF05_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZW51277.1"
                     /translation="MSATPQAESVQVRQCAGSGRVNRHSATIATRIGVDADSAHGAVM
                     PPLYLSSNYSFAGFGRKRRYDYTRSGNPTRDTLAGALTELEGGAGGVVTASGMAAIAL
                     LTAQLKPGDLLVAPHDCYGGSYRLFRSQSARGLYAVEFVDFTDPAALSAALAHKPKLV
                     WVETPSNPLLRITDIAATCASAHAFGALVAVDNTFLSPALQQPLALGADLVVHSTTKY
                     LNGHSDVVGGAVVWKDAGLGAELAWWCNCLGLAGAPFDSYLTLRGLRTLGPRLAAHQA
                     NAIALAELLIENDAVKKVYFPGLSRHPGHLLARRQQRGFGAMLSFDLAGGEAAAAAFV
                     EGL"
BASE COUNT          209 a          393 c          402 g          198 t
ORIGIN      
        1 cagaccttcg acaaaggctg ccgccgcggc ttcgccgcca gccagatcga aactcagcat
       61 cgcgccgaat ccacgttgct gacggcgcgc cagcagatgg cccggatggc gactcaggcc
      121 cggaaagtac actttcttca ctgcatcgtt ctcgatcagc aattccgcca gcgcgattgc
      181 gttggcctgg tgcgcggcca ggcgcggtcc gagcgtgcgc aggccgcgca aggtgaggta
      241 actgtcgaag ggtgcgccgg ccaggcccag gcaattgcac caccaggcca gttccgcgcc
      301 gagtcccgcg tccttccaca ccaccgcgcc accgaccacg tcggaatggc cgttgaggta
      361 tttcgtggtc gagtgcacga ccaggtccgc gccgagcgca agcggttgct gcaatgccgg
      421 cgacaggaat gtgttgtcga ccgcaacgag ggcgccgaat gcatgcgcgc tcgcgcacgt
      481 cgccgcgatg tcggtgatgc gcagcaacgg attgctcggc gtttcgaccc agaccagttt
      541 cggtttgtgc gcgagtgccg cagacaacgc cgcgggatcg gtgaagtcga cgaactcgac
      601 ggcgtacagg ccacgcgcgg actgcgagcg gaacaggcga tagctgccgc cgtagcagtc
      661 gtgcggcgcg accagcagat cgccgggctt gagttgcgcg gtcagcagcg cgatggcggc
      721 cattccagac gcagtcacca cgccgccggc accgccctcg agttcggtca gtgctccagc
      781 cagcgtatcg cgcgtgggat tgccggagcg cgtgtagtcg tagcgacgtt tgcgcccgaa
      841 tccggcgaaa ctgtaattgc tcgacaggta caacggcggc atgaccgcgc catgtgcgct
      901 gtccgcatcg acgccgatgc gtgtcgcgat ggtcgcgctg tggcggttca cgcgtccgct
      961 cccggcgcat tggcgcacct gcacggattc ggcttgcgga gttgcggaca tgcggcgccc
     1021 tcggtcgagt gacgcgcatc ctaccttcag catttatgga cgtcaagccg tctagacgtc
     1081 tatttcattt cacgctggtt cggcaacggg aacgggtcgc cggcgcgccg agccgatgaa
     1141 gatgccgagc agggacaggg ccaggccgat ccagtccagc gctccggtcg gacgcgcgaa
     1201 ca
//
LOCUS       DASRVO010000436         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_64715, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000436 DASRVO010000000
VERSION     DASRVO010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64715"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFF05_05435"
     CDS             complement(<1..682)
                     /locus_tag="VFF05_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZW51278.1"
                     /translation="MTSDLVSQFRRLARNGDARAVSYVRLRQALLATLEGGGLQAGQA
                     LPGERELARQLDLSRVTVRRAIAGLVDDGVLIQRHGAGTFVAERIVKSFSRLTGFTDD
                     LRARGLKPRVRFLERGSGEVTPAEALALHLSPGTRVVRMRRLRYADDTPLALEDTSVP
                     QSMLRDPKRVRQSLYDTLEKLGCRPVRALQRLRAVALDAAQAKLLDLPPGSPALAIER
                     RAFLADGRV"
     gene            804..>1862
                     /gene="zwf"
                     /locus_tag="VFF05_05440"
     CDS             804..>1862
                     /gene="zwf"
                     /locus_tag="VFF05_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZW51279.1"
                     /translation="MGTSPPVEDFDLAIFGGTGDLALRKLLPALLHRFADGQIGAASR
                     IIGVARDAMSDVEYRERVRPALAAAADGYAATTDRFLALLSYRALDAMRDADWDEFTR
                     YLNAPGDRVRVFYLATAADLFVPICERLRELGLTEGKVRVVVEKPIGRDLASAAAIND
                     AIGRTFAENQTFRIDHYLGKETVQNLLALRFGNALFEPLWRAEHIDHVQITVAETIGV
                     ERRAAFYAGAGALRDMVQNHLLQLLCMVAMEPPVSLEPDAVRDEKLKVLNALRPLGAD
                     AARLTVRGQYRAGAIGTGPVAGYDEENGGGHARTETFVALKAEVANWRWSGVPFYLRT
                     GKRLAQRVSEIVIAFRAIP"
BASE COUNT          311 a          599 c          643 g          309 t
ORIGIN      
        1 cgacgcggcc gtcggcaaga aacgcgcggc gttcgatcgc gagcgcggga ctgcccggcg
       61 gcaaatccag caacttcgcc tgcgccgcat cgagggcgac ggcgcgcaac cgctgcaatg
      121 cgcgcaccgg gcgacagccg agtttttcaa gcgtgtcgta cagcgattgc ctgacccgtt
      181 tcggatcgcg cagcatcgac tgcggtacgg acgtatcctc caaagccagc ggggtgtcgt
      241 ctgcgtaacg caggcgccgc attcgcacca cgcgcgtacc tggcgagagg tgcagggcca
      301 gcgcctcggc cggggtgact tcgccgctgc cgcgttcgag gaaacgcacg cgcggcttga
      361 ggccgcgcgc gcgcaggtcg tcggtgaagc cggtcaggcg cgaaaacgat ttgacgatgc
      421 gctcggcgac gaaagtgccg gcgccgtggc gctggatgag cacgccatcg tcgaccaatc
      481 ccgcgatcgc gcggcgcacg gtcacccgcg acagatcgag ctgacgcgcc agctcgcgct
      541 cgcccggcag cgcctgaccc gcttgcagtc cgccgccttc cagcgtggcc agcagggcct
      601 ggcgcaggcg cacgtagctc acggcgcgtg cgtcgccgtt gcgggcgagg cggcggaact
      661 gggagaccag gtcggatgtc atgccgttga acataccact tgaataccac gatgcgcaaa
      721 catgacatcc agtctggaag agttgcgcca tgtggtattc aagtggtatt ttgcgtgcag
      781 acattttctc gacggagtga agaatgggta caagtccgcc ggttgaagac ttcgatctgg
      841 cgatcttcgg cggcaccggc gatctggccc tgcgcaagct gttgccggcg ctgctgcacc
      901 gcttcgccga cggccagatc ggtgcggcca gccgcattat cggcgtggcg cgcgacgcga
      961 tgagcgacgt tgaatatcgc gagcgcgtgc gcccggcatt ggctgccgcg gcggacggct
     1021 atgcggcaac cacggatcgc ttcctcgcgt tgctgagcta tcgggcgctc gatgccatgc
     1081 gtgatgcgga ctgggacgag ttcacgcgtt atttgaacgc gccgggcgac cgcgtgcggg
     1141 ttttctatct cgccaccgcg gcggatttgt tcgtgccgat ctgcgagcgc ctgcgcgaac
     1201 tcggcctgac cgagggcaag gtgcgcgtgg tcgtggaaaa acccatcggc cgtgatcttg
     1261 caagcgccgc agcgatcaac gacgcgatcg gacgcacgtt cgccgagaac cagaccttcc
     1321 gcatcgacca ttatctgggc aaggagacgg tgcagaacct gctcgcgctg cgcttcggca
     1381 acgcgttgtt cgagccgttg tggcgcgccg agcacatcga tcatgtgcag atcacggtgg
     1441 ccgaaaccat cggcgtggag cgccgcgccg cgttttacgc gggcgccggc gcgctgcgcg
     1501 acatggtaca gaaccacttg ctgcaattgc tgtgcatggt cgcgatggag ccgccggttt
     1561 cgctcgagcc cgacgcggtg cgcgacgaga aactgaaagt cctcaacgca ctgcggccgc
     1621 tcggtgccga cgccgcgcgc ctgaccgtgc gtgggcaata ccgcgccggc gcgatcggta
     1681 ctggaccggt ggccggctac gatgaggaga atggcggcgg ccacgcgcgc accgagacct
     1741 tcgttgcgct caaggccgag gtcgcgaact ggcgctggtc gggcgtgccg ttttacctgc
     1801 gcaccggcaa gcgcctggcg cagcgcgtat ccgaaatcgt gatcgcgttc cgcgcgattc
     1861 cg
//
LOCUS       DASRVO010000437         1113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_220752, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000437 DASRVO010000000
VERSION     DASRVO010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220752"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>1113
                     /locus_tag="VFF05_05445"
     CDS             102..>1113
                     /locus_tag="VFF05_05445"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HZW51280.1"
                     /translation="MTECFAEYVWLPQGWGESTRFRIEAGALTEVDESANGAGVNRIG
                     RFVLPGMPNLHSHAFQRAMAGLAEKQWNPQDNFWSWRETMYAFAGRVGPDDLRAIAAQ
                     LYVEMLKAGYTQVCEFHYLHHQPDGKPYADPAAMSLALIEAAREAGIGLTLLPVLYMT
                     GGFDGRPLSERQCRFGFDVDGYLRLGEKLRKMETSTLRVGMALHSLRAVPTDAMRVVL
                     ESSLADGPVHIHIAEQIGEVQDCLNVRGARPVEWLFDNAPVDARWCLVHATHMTAVET
                     RRLAESGAVSGLCPTTEANLGDGLFPLAAYRAAGGVLGIGSDSNISISPIEELRWLEY
                     GQR"
BASE COUNT          190 a          342 c          362 g          219 t
ORIGIN      
        1 tgtgtcggtc tccggattgg ctggcaggca tgcgtgtatt atcgcagact ttgatttccg
       61 cacagcacac cgaattcgcc caccggctgg aatccgccgc aatgaccgag tgtttcgccg
      121 aatacgtctg gctgccgcag ggctggggtg aaagcacgcg ctttcgcatc gaagcgggcg
      181 cgttgaccga agtagacgaa agcgcgaacg gtgccggcgt gaatcgaatc ggccgcttcg
      241 tgctgcccgg catgccgaac ctgcattcgc acgcgttcca gcgcgcgatg gcgggcctcg
      301 cggaaaagca gtggaatccc caggacaatt tctggtcctg gcgcgaaacc atgtacgcct
      361 tcgccggccg cgtcggcccg gacgatctgc gcgcgatcgc cgcgcagttg tacgtggaga
      421 tgctcaaggc cggttacacg caggtttgcg aattccatta tttgcatcac cagccggacg
      481 gcaagcccta tgccgatcca gcggcgatgt cgctggcatt gatcgaggcc gcgcgcgagg
      541 cgggcatcgg attgacgctg ttgccggtgc tgtacatgac cggcggtttc gatggccggc
      601 ccttgtccga acgccaatgc cgcttcggct tcgacgtgga cggctatctg cgtctcggcg
      661 aaaaattacg caagatggaa acttccacat tgcgcgtcgg catggcgctg cactcgctgc
      721 gcgccgtgcc gacggatgcc atgcgcgtgg tattggaatc cagtcttgcc gatgggccgg
      781 tacatatcca catcgccgag caaatcggcg aggtgcagga ttgtttgaac gtgcgtggcg
      841 cgcggccggt cgaatggttg ttcgacaacg cgcccgtcga tgcgcgctgg tgcctggtcc
      901 acgccaccca catgaccgcg gttgaaacgc gccgcctcgc cgaatccggc gcggtgtctg
      961 ggctgtgtcc gaccacggaa gcgaatctcg gcgacggatt gttcccgctg gccgcctacc
     1021 gcgcggcggg cggtgtgctc ggcatcggtt cggattccaa tatttccatt tcgccgatcg
     1081 aggaattgcg ctggctcgaa tacggccagc gcc
//
LOCUS       DASRVO010000438         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_246427, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000438 DASRVO010000000
VERSION     DASRVO010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246427"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFF05_05450"
     CDS             complement(<1..561)
                     /locus_tag="VFF05_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZW51281.1"
                     /translation="MNLDTKQSSPGVRLATVPDDREGQRIDNFLTGQLKGVPKSLIYR
                     LLRTGQVRINGKRAKPDARISGGDTVRIPPVRTAAEGESGPAPKAMVARVAQSIIFED
                     RDFLVIDKPAGIASHGGSGVAFGAIELLRAARPDETLELAHRLDRDTSGVLVLARRRA
                     ALTALQELIRDGHITKQYLALLHGHPK"
     gene            936..>2055
                     /locus_tag="VFF05_05455"
     CDS             936..>2055
                     /locus_tag="VFF05_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZW51282.1"
                     /translation="MKRMLINATQREELRVAIVDGQTLYDLDLEIPSKEQKKANIYKG
                     RITRVEPSLEACFVEYGADRHGFLPLKEISRDYFQAGADHNKAGIRELLKEGQELLVQ
                     VEKEERGNKGAALTTFVSLAGRYMVLMPNNPRAGGVSRRIEGDDRANLKEILDQLKLP
                     DDMGLIIRTAGLGREAEELQWDLDYLLQLWTAIDEAGKSRPAPFLIYQESRLIIRALR
                     DYLRNDIGEILIDSEELFNDAREFVQQVMPQNLRKLKLYKDTVPLFSRFQIETQIENA
                     FDRTVRLPSGGSIVIDQTEALTSIDINSSKATKGADIEETAFNTNCEAAVEVARQLRL
                     RDAGGLIVIDFIDMDNPRHQREVEDKLKDALRFDRARVQ"
BASE COUNT          400 a          680 c          642 g          333 t
ORIGIN      
        1 cttcggatgc ccatgcagca gcgccagata ctgtttggtg atgtggccat cgcgaatcag
       61 ttcctgcagc gcggtcagcg cggcgcgccg gcgcgcgagg acgagcacgc cgctggtatc
      121 gcgatcgagc cgatgggcca gttccagggt ttcgtcggga cgcgccgcac gcagcagttc
      181 gatggcgccg aaagcgacac cgctgccgcc atgactggcg atgccggccg gcttgtcgat
      241 aacgaggaag tcccgatcct cgaaaatgat cgattgcgcc acccgcgcga ccattgcctt
      301 cggcgccggc ccggattcgc cctcggcggc cgtccgcacc ggcggaatcc gcaccgtatc
      361 gccgccggat atgcgggcat ccggtttggc gcgcttgccg ttgatccgca cctgcccggt
      421 gcgcagcaag cggtagatca ggctcttggg cacgcccttg agttgtccgg tcagaaagtt
      481 gtcgatgcgc tgcccttcac ggtcgtcggg gaccgtcgcc aggcgcacgc cggggcttga
      541 ttgctttgta tcgagattca tcgaagctcg cgtaagtatt ggaaaaatcg gccgctgtgc
      601 tacacttcgg ccgcgtttcg cgtcagcgcc ggtcagacgg cgtgacgacg catccgccca
      661 ccggcatgcg ccggcgtccg cgccaactcc gggaggagtc ctcggccgcc gcgtaatcct
      721 aacgggtcgg atcgcggatt gcccgtctcc gtcatggcgg cgtggagtcc cggcaacgca
      781 aaaagggcac ctttcaaccg cctggcgcag cagtgcgccg gcagataaaa agcgctcccg
      841 tggtggcgtg ccacgcccac ggcttgcgcg gctccgccgc cgaattcggc gcggaatcgc
      901 gacgcggtcc acggtcgagc gccgaggatg taaaaatgaa gcgtatgctg atcaacgcaa
      961 ctcagcgtga agagttgcgc gtggccatcg tcgacggcca aaccctgtac gatctcgatc
     1021 tcgagatccc ctccaaggaa cagaaaaagg ccaatatcta caaaggccga attacccgcg
     1081 tcgaaccgtc gctggaagcc tgtttcgtcg aatatggcgc cgaccgccac ggcttcttgc
     1141 cgctcaagga aatctcgcgc gattacttcc aggccggcgc cgaccacaac aaggccggca
     1201 tccgcgagtt gctcaaggaa ggccaggaac tgctcgtcca ggtcgaaaag gaagagcgcg
     1261 gcaacaaggg cgccgcgctg accacgttcg tctcgctggc cggccgctac atggtcctga
     1321 tgccgaacaa cccgcgtgcc ggtggcgtgt cgcgccgcat cgagggcgac gaccgcgcga
     1381 acctgaagga aattctcgac cagctcaagc tgccggacga catgggcctg atcatccgca
     1441 ccgccggcct cggccgcgag gcggaagaac tgcaatggga cctggattac ctgctgcagt
     1501 tgtggacggc gatcgacgag gccggcaaat cgcggcccgc gccattcctg atctaccagg
     1561 aatcgcgcct gatcatccgc gccctgcgcg actacctgcg caacgacatc ggcgagatcc
     1621 tgatcgactc ggaagaactc ttcaacgacg cgcgcgaatt cgtgcagcag gtcatgccgc
     1681 agaacctgcg caagctcaag ctgtacaagg acaccgtacc gctgttctcg cgcttccaga
     1741 tcgaaaccca gatcgagaac gcgttcgatc gcacggtgcg cctgccatcc ggcggctcga
     1801 tcgtgatcga ccagaccgag gcgctcacgt cgatcgacat caactcgtcg aaggcgacga
     1861 aaggcgccga catcgaagaa accgcgttca acacgaactg cgaggcggcg gtcgaagtcg
     1921 cgcgccagtt gcgcctgcgc gatgccggcg gcctgatcgt catcgatttc atcgacatgg
     1981 acaacccgcg ccaccagcgc gaggtcgaag acaagctcaa ggacgcgctg cgcttcgatc
     2041 gcgcgcgcgt ccagg
//
LOCUS       DASRVO010000439         3296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_116765, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000439 DASRVO010000000
VERSION     DASRVO010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116765"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..2056
                     /locus_tag="VFF05_05460"
     CDS             104..2056
                     /locus_tag="VFF05_05460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZW51283.1"
                     /translation="MRIASARELLGADGPFAAEVSGFVPRACQQDMAEAVEEAIAERQ
                     MLIVEAGTGTGKTFAYLVPALLSGKRVIVSTGTKTLQDQLFHRDLPRVRTVLGTRLKT
                     SLLKGRANYLCRYRLEQTLKDGRFASREQASQVQAIRAWGERTASGDRGEFSDVPEDS
                     PLWPRVTSTAENCLGAECPLYADCFVVKARRAAQEADLVVVNHHLLFADLAIKQEGFG
                     EILPGAHAFVLDEAHQIPELAGVFFSTSITARQMTELASDTLAECASVSGALATLREP
                     FEAIAPAVRRVRLALDALPAKGAFAQIEQNAGALSDLHALEDALRKLADALGAQAERS
                     RGMASAHERAEALCQRLHTVIDGSAHDAVQWYETTTHGFALHATPLDLAPPLRALREQ
                     SLASWIFTSATLSVAGSFEHFSRQLGLDDPVEVNLPSPFDYATQALAYLPRSLPDPSA
                     PDYTERVVAAARPVIAAARGRTFVLFTSHRALKEAAELLGDLPYPLFVQGTAPRHQLL
                     TDFRNSGNGVLLGAASFWEGVDVAGEALSCVIIDKLPFAAPDDPVLEARLSALRESGA
                     NPFFAWQVPAAAIALKQGAGRLIRDAGDRGVLMLCDPRLTTRGYGKLFLQSLPPMSRT
                     RELADVQAFFAVDALPAADASLADLG"
     gene            2064..2888
                     /locus_tag="VFF05_05465"
     CDS             2064..2888
                     /locus_tag="VFF05_05465"
                     /inference="COORDINATES: protein motif:HMM:NF006734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HZW51284.1"
                     /translation="MNADAHSAAARLELNGAPAGVDGLRHLVQTNYGHFTVMRVEDGC
                     VRGLDLHLDRLQSATRELFGSELDCERVRGYLRHALSGATGAWSLRVNVFARALDREH
                     MDRTVDVDVLVGANPAAVAATQPLRVKSFVYAREAPQIKHVGTFALFQHRRLARLAGF
                     DDALFVDADGCISEGSIWNIGFVDRDGVVVWPQAPQLGGVGMRLVDAGLPSQDLSSRV
                     RPVYLGELATFRAAFFSNASVLVRMLASIDACRFEVDSEIERRLLAAYQSNPPQTI"
     gene            2944..>3296
                     /gene="tsaB"
                     /locus_tag="VFF05_05470"
     CDS             2944..>3296
                     /gene="tsaB"
                     /locus_tag="VFF05_05470"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011258504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZW51285.1"
                     /translation="MSNIIAIETATEACSAALSVDGRVVERSEIAPRRHAELILPMIE
                     SLLVEAGISRAQLDGIAVGRGPGAFTGVRLAIAVAQGLALALDIPIVPVSSLAALAQD
                     APDDGASILAVIDAR"
BASE COUNT          513 a         1097 c         1104 g          582 t
ORIGIN      
        1 cgcaggcgcg tgcgctcacc gtgcagggcg acgaggccga agcggtgcag gctcgcgcaa
       61 aaatcgaagc ctgccgcgtg ccgccggtga cgcggttctg agcatgcgca tcgccagcgc
      121 gcgcgagctg cttggcgcgg atggaccgtt cgcggcggaa gtttccggtt ttgtgccgcg
      181 cgcctgccag caggacatgg ctgaagcggt cgaggaagcg atcgccgaac ggcagatgct
      241 gattgtcgag gcgggcaccg gcaccgggaa aacgtttgcg tatctggttc cggcgctact
      301 gtccggcaag cgcgtgatcg tttccaccgg aaccaagacc ctgcaggacc agttgttcca
      361 tcgcgacctg ccgcgcgtgc gcaccgtgct cggcacgcgc ctgaagacga gcctgctcaa
      421 gggccgcgcg aattacctgt gccgttatcg actcgaacag acgctgaaag acggacgctt
      481 cgcctcgcgc gaacaggcga gccaggtgca ggcgatacgc gcctggggcg aacgcaccgc
      541 gagcggcgac cgcggcgagt tttccgacgt gccggaagat tcgccgctgt ggccgcgcgt
      601 gacctcgacc gcggaaaact gccttggcgc cgagtgtccg ctgtacgcgg attgcttcgt
      661 ggtcaaggcg cgacgcgcgg cgcaggaagc cgaccttgtc gtcgtcaacc atcatctgct
      721 gtttgccgac ctcgcgatca agcaggaagg cttcggcgaa atcctgccgg gcgcgcacgc
      781 cttcgtgctc gacgaggcgc accagattcc ggaactggcc ggcgtattct tttccacaag
      841 tataaccgcg cgccagatga cagaactggc cagcgacacg ctggccgaat gcgcgagcgt
      901 atccggtgcg ctggccacgt tgcgcgaacc gttcgaggcg atcgctccgg cggtgcggcg
      961 cgtgcgcctg gcgctggatg cgctgccggc gaagggcgcc ttcgcgcaga tcgaacagaa
     1021 cgccggtgcg ctatccgatt tgcacgcgct ggaagacgcc ttgcgcaagc tcgccgatgc
     1081 gctcggcgcg caggccgagc gttcgcgcgg aatggccagc gcgcacgaac gcgccgaagc
     1141 gctgtgccag cgcttgcaca cggtcatcga cggcagcgct cacgacgccg tgcaatggta
     1201 cgagacgacg acgcacgggt tcgccctgca tgcgacgccg ctggacttgg cgccgccgtt
     1261 gcgcgccttg cgtgaacaat cactcgccag ctggattttc acctcggcca cgttgtcggt
     1321 tgccggcagt ttcgagcatt tctcgcgcca gctcggcctc gacgatccgg tggaagtgaa
     1381 cctgcccagt cccttcgatt atgcgacgca agcgctcgcc tatctgccca ggagcctgcc
     1441 cgatccgagc gctccggact acaccgagcg cgtggtcgcc gcggctcggc cggtcatcgc
     1501 cgccgcgcgc gggcgcacgt tcgtgctttt cacatcgcac cgcgcgctga aggaagcggc
     1561 ggagttgctc ggcgacctgc catatccgtt gttcgtgcag ggcaccgcgc cacgccacca
     1621 gttgctcacc gacttccgca attccggcaa cggggtgctg ctcggcgccg cgagtttctg
     1681 ggaaggcgtc gacgttgccg gcgaggcgct ttcctgcgtc atcatcgaca agctgccgtt
     1741 cgccgcgccg gatgatccag tgctggaagc gcgcctttcc gcgttgcgcg aatccggcgc
     1801 aaatcccttc ttcgcctggc aggtgccggc tgcggcgatt gcgctcaagc agggtgcggg
     1861 acgcctgatc cgcgatgccg gcgaccgcgg cgtgctgatg ctgtgcgatc cgcgcctgac
     1921 cacgcgcggc tacggcaaat tgttcctgca aagcctgccg ccgatgtcgc gcactcgcga
     1981 actcgccgac gtgcaggcgt tcttcgcggt ggatgcgctg cctgccgccg atgcgtcgct
     2041 ggctgacctg ggttgatgcg cgagtgaacg cagacgcaca ttcggccgca gcgcgactcg
     2101 aattgaacgg cgcgccggcg ggcgtggacg gcttgcgtca tctcgtgcag acgaactacg
     2161 gccacttcac cgtcatgcgc gtcgaggacg gctgcgtgcg cgggctagac ctgcacctgg
     2221 atcgcctgca atcggcgaca cgcgagctgt tcggcagcga gctcgactgc gaacgcgtgc
     2281 gcggctattt gcggcatgcg ctttccggcg cgaccggtgc gtggtcgctg cgcgtcaacg
     2341 tgttcgcgcg cgcgctcgac cgcgaacaca tggatagaac ggtggacgtc gacgtgctgg
     2401 tcggtgcgaa tcccgctgcc gtcgcggcga cacagccgct gcgggtcaag tccttcgtct
     2461 acgcgcgcga ggcgccgcag atcaaacacg tcggcacgtt cgcgctgttc cagcatcggc
     2521 gtctggcgcg gctggccggt ttcgacgatg cgctgttcgt cgatgccgat ggctgcatca
     2581 gcgaaggctc gatctggaac attggattcg tcgatcgcga tggcgtggtc gtgtggccgc
     2641 aggcaccgca actcggtggc gtcggcatgc gcctggtcga tgccgggctg ccgagtcagg
     2701 acttgagcag ccgcgtgcgt ccggtgtacc taggcgaact cgcgacgttc cgcgccgcct
     2761 tcttcagcaa cgccagcgtg ctggtgcgga tgcttgcgtc catcgacgct tgccgtttcg
     2821 aggtcgattc agaaatcgaa cggcgcctgc tggcggctta ccaatccaat ccgccacaga
     2881 cgatctgatc ggcgcgtgcg aaccagggtt ccgtcgccgc gccatagccg ttacgatgcc
     2941 gccatgtcga acatcatcgc catagaaacc gccaccgaag cctgttcggc cgccttgagc
     3001 gtcgatggcc gcgtcgtcga acgcagcgag atcgcgccgc gtcggcacgc cgaattgatc
     3061 ctgcccatga tcgaatcgct gctcgtcgaa gcggggatct cacgcgcgca gctcgacggc
     3121 attgccgtcg gccgcggacc cggtgcgttt accggcgtgc gtctcgccat cgcggtggcg
     3181 caaggactcg cattggcact ggatattccc atcgttccgg tttcctcgct tgccgcactc
     3241 gcgcaggatg ccccggatga cggcgcgtcg attctcgcgg tgatcgacgc tcgcat
//
LOCUS       DASRVO010000440         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_142231, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000440 DASRVO010000000
VERSION     DASRVO010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142231"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..604)
                     /locus_tag="VFF05_05475"
     CDS             complement(98..604)
                     /locus_tag="VFF05_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51286.1"
                     /translation="MKSLSRAAAPWRLLKSRGRSARGPEPPRIVLDGCEDIEGRALLL
                     VSALHFGAAALAGWKVHFVVQGDAAVCAALDALRWDTGLDVEWLHDRQRDAVLPGARL
                     FAAVCFRDVTRLNLEDIARHGVPALVAIQFPDFERHGAEALALQRAAHDPQLLCAEIL
                     ARLGVALP"
     gene            complement(601..>1062)
                     /locus_tag="VFF05_05480"
     CDS             complement(601..>1062)
                     /locus_tag="VFF05_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZW51287.1"
                     /translation="VPLGTCGPVGGVLDRMAEDFLVMNGDLLTDIDLTQFMRLHRDSS
                     CAATVAGAMHTQQLDYGVLDVDASGRLRGYREKPQTEWLVSTGIYALRRDAVREFVPA
                     GSRLDMPELLERLIAAELPVHVWRGTCRWQDIGRPEEYAAAQLWSESGSPM"
BASE COUNT          205 a          378 c          323 g          156 t
ORIGIN      
        1 aatggacccg cgcgtaccgc cttcaaccag cgtgccgcgt gcacactgcc gatcatgccg
       61 acgacggcga tcaacggctg tggatcgtgc ggcgccgtca cggcagggca acgcccaggc
      121 gcgcgagaat ttccgcgcag agcagctgag gatcatgggc ggcgcgctgc agcgccagcg
      181 cctcggcgcc gtgccgctcg aagtccggaa actggatcgc caccagtgcg ggtactccat
      241 ggcgggcgat gtcctccaga ttcaggcgcg taacgtcgcg aaagcaaacg gcggcgaaca
      301 ggcgcgcacc gggcaatacc gcgtcgcgtt gccggtcatg cagccattcc acatccaggc
      361 cggtgtccca gcgcaacgcg tcgagcgcgg cgcagaccgc agcatcgcct tgcaccacaa
      421 aatggacttt ccagccggca agcgccgctg cgccgaaatg cagagccgaa accagcagca
      481 gggcccggcc ttcgatgtct tcgcatccat cgagtacgat gcgcggcggc tcgggtccgc
      541 gcgcgctgcg accgcgcgac ttcaataaac gccagggcgc cgcggcgcgc gacagcgatt
      601 tcacatcgga cttccggact cggaccacaa ctgcgctgcc gcgtattcct caggacgtcc
      661 gatgtcctgc caacggcatg tcccgcgcca gacgtgcacc ggaagctcag ccgcaatcag
      721 tctttccagc agctccggca tatcgagacg gctgccggcc ggaacgaatt cgcgaactgc
      781 atcgcgccgc agcgcgtaga ttcccgtgct caccagccat tccgtctgcg gcttttcgcg
      841 ataccctcgc aggcggcccg acgcgtcgac gtcgagcaca ccgtagtcaa gctgctgcgt
      901 gtgcatcgcg ccggccacgg tagccgcaca tgacgagtct cgatgcaacc tcatgaactg
      961 ggtgagatcg atatcggtaa gcagatcccc attcatcacc agaaaatcct ccgccatccg
     1021 atccagcacg ccgccgaccg ggccgcaggt gccgagcggc ac
//
LOCUS       DASRVO010000441         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_220995, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000441 DASRVO010000000
VERSION     DASRVO010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220995"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="tdh"
                     /locus_tag="VFF05_05485"
     CDS             <1..753
                     /gene="tdh"
                     /locus_tag="VFF05_05485"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010378413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HZW51288.1"
                     /translation="IVCGVCRNCRAGKQHLCPNTVGIGVNRNGAFAEYIAMPSSNLWP
                     IPDQIPSELAAFFDPYGNAAHCALEFDLIGEDVLITGAGPIGIIAAGIAKHVGARNVV
                     VTDVNDYRLKLAADMGATRVVNVSKQSLKDVVKDLHMEGFDIGLEMSGNPRAFNDMLD
                     CMYHGGKIALLGILPNGSGIDWDKVIFKGLVLHGIYGRRMYETWYKMTQMLLTGFPLQ
                     KVLTHQVHIDDFQKGFDLMEAGNCGKVVCSWN"
     gene            805..>2168
                     /locus_tag="VFF05_05490"
     CDS             805..>2168
                     /locus_tag="VFF05_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51289.1"
                     /translation="MTFSRLLALCPLVFACSAHASDAFEPEPVLKAPMLVQAPLLSGP
                     DFRVVPEVAVRGYMANFLIDTKFGPLRADSVEMLAIRIGELPAMEALDEASRSGAFAT
                     ALAARAKKTGFAVVNVIAHPIDTLTGLPRGVARFLGAQWDKWSGRAQSLSDRSQREFE
                     NRGDPYRAPAGPMTAMRDAPADSPPAPADAKNRAWYARAGSEVAREGKRYLKFNSQRR
                     AMAKLLGVDPNSSNPVLDQELDTLAWAAVWGNFSAGTALGQIGGSAATVISDTGKLNQ
                     YVLSKTPEQLRADNEKRLLALCSDETAVRSFLRRGGFTDTLRTALALTLEKLAPKSGC
                     DGLLELAAKTHGEVEARYLVNALKLVAQQPDARNGELVAAGAAIVWRMPSGKLILPLP
                     VDYLTWNADLAAFFDQPRLRAASRLVLIGGEASMLAQRALTERGWGLQLRAPYEGSPA
                     YAMNG"
BASE COUNT          399 a          719 c          682 g          368 t
ORIGIN      
        1 atcgtctgcg gcgtctgccg caactgccgc gccggcaagc agcacctgtg cccgaacacg
       61 gtcggcatcg gcgtcaatcg caacggcgcg ttcgccgaat acatcgccat gccgtcgtcc
      121 aacctgtggc cgattccgga ccagattccg tccgaactcg ccgcgttttt cgatccctac
      181 ggcaacgccg cgcactgcgc gctggaattc gacctgatcg gcgaagacgt gctgatcacc
      241 ggtgcgggtc cgatcggcat catcgccgcc ggcatcgcca agcacgtcgg cgcgcgcaat
      301 gtcgtggtca ccgacgtcaa cgattatcgc ctcaagctcg ccgccgacat gggcgcgacg
      361 cgcgtggtca acgtctcgaa gcaatcgctg aaagacgtcg tcaaggacct gcacatggaa
      421 ggcttcgata tcggcctgga aatgtccggc aatccgcgcg ccttcaacga catgctcgac
      481 tgcatgtacc acggcggcaa gatcgcgctg ctcggaatcc tgccgaacgg ttcgggcatc
      541 gactgggaca aggtcatctt caagggcctg gtcctgcacg gcatctacgg ccggcgcatg
      601 tacgagacct ggtacaagat gacgcagatg ctgctgaccg gctttccgct gcagaaggtg
      661 ctcacccacc aggtgcacat cgatgatttc cagaaaggtt tcgacctgat ggaagcgggc
      721 aattgcggga aagtggtttg ctcgtggaat tgagctagct cgtcgttcag cgccgattgg
      781 cgctgattgc ggcatcgtga cgcgatgacg ttttcacgat tgcttgcgct ctgcccgctc
      841 gtcttcgcct gctccgccca cgcgagcgac gctttcgagc ccgaacccgt gctcaaggcg
      901 ccgatgctgg tgcaggcgcc gctgctttcc ggtccggatt tccgcgtggt gcctgaggtc
      961 gcggtgcgcg gttacatggc gaatttcctg atcgacacga agttcggtcc gctgcgcgca
     1021 gacagcgtgg aaatgcttgc gatccgtatc ggcgaattgc cggcgatgga agcgctcgac
     1081 gaggcgagcc gcagcggcgc gttcgcgacc gcgcttgccg cgcgcgcgaa aaaaactgga
     1141 ttcgccgtcg tcaacgtcat cgcccatccg atcgataccc ttaccggcct gccgcgcggc
     1201 gtggcgcgct ttctcggcgc gcagtgggac aaatggagcg gccgcgcgca atccttgtcc
     1261 gaccgctccc agcgcgaatt cgaaaaccgc ggcgatccct atcgcgcgcc tgccgggccg
     1321 atgacagcga tgcgcgacgc accggccgac agtccgccag cgccagccga cgcaaaaaac
     1381 cgtgcctggt acgcacgcgc cggcagcgaa gtggcgcgcg aaggcaagcg ctacctgaaa
     1441 ttcaactcgc aacggcgcgc catggccaag cttctgggcg tggatccgaa ctcgagcaat
     1501 ccggtgctcg accaggaact cgataccctg gcctgggccg cggtgtgggg gaatttttcc
     1561 gccggcaccg cgctcggcca gatcggcggc agcgcggcga cggtaatcag cgacaccggc
     1621 aagctcaacc aatacgtgct gtcgaaaacg cccgagcaat tgcgcgcgga caacgaaaag
     1681 cgcctgctcg cgctgtgcag cgacgaaacc gcggtgcgca gcttcttgcg ccgtggcggc
     1741 ttcaccgaca cgctgcgcac tgcgctggca cttacgctcg aaaaactcgc gccgaaatcc
     1801 ggctgcgacg gcctgctcga actcgctgcg aaaacgcacg gcgaagtcga agcccgctat
     1861 ctcgtcaatg cgctcaagct cgtcgcgcag caacccgacg cgaggaacgg cgaactggtc
     1921 gcggcgggcg ccgccattgt ctggcgcatg ccatccggca aactgatcct gccgctgccg
     1981 gtggattacc tgacgtggaa cgccgatctc gccgcgttct tcgaccagcc gaggctgcgc
     2041 gcagcgagca gactggtgct gatcggcggc gaggcgtcga tgctggcaca gcgcgcgctt
     2101 accgagcgcg gctggggact gcaattgcgc gccccctacg agggttcacc cgcatatgcg
     2161 atgaacgg
//
LOCUS       DASRVO010000442         1071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_13121, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000442 DASRVO010000000
VERSION     DASRVO010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13121"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1071)
                     /locus_tag="VFF05_05495"
     CDS             complement(<1..>1071)
                     /locus_tag="VFF05_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HZW51290.1"
                     /translation="SCSINGSPPIKTRRQLDPAEIAVIGHALHSVAVEMGAALRRTAF
                     SPNIKERRDYSSAVFSADGDLIAMGDDMPVHLGSMPMSVAAVLADLRLAPGDVALLND
                     PYRGGTHLPDITMVAPVFLPRRNRAAFFVANRAHHADVGGMYPGSMGPCREIAQEGIR
                     IPPVKLMHAGKMDTQMLATILANVRTPKEREGDLTAQLGACRIGATRMLQLVERFGYA
                     RLLQGVGAMLTGSERLMRNVLAALPAGEWAAEDFLDDDGVIPGPFAIRVKIANDPKRR
                     RALVDFAGTDPQVPGSINAVYAITWSAVFYVFRCLLPPGAIATAGLMRPVQVLAPQGS
                     IVNARPPAAVAGGNVETSQRIVD"
BASE COUNT          204 a          295 c          393 g          179 t
ORIGIN      
        1 atcgacgatg cgctgtgagg tttccacatt gccgccggcg acagccgcgg gcggacgcgc
       61 gttgacgatc gatccttgcg gcgcgagtac ctggacggga cgcatgagtc ctgcagtggc
      121 gatggcgccg ggcggaagca ggcaacggaa gacatagaag accgccgacc aggtgatggc
      181 atagacggcg ttgatgctgc ctggaacctg cggatcggta ccggcgaagt cgacgagggc
      241 gcgtcggcgt ttgggatcat tcgcaatctt gacgcgaatg gcaaacgggc cggggatcac
      301 gccgtcgtcg tcgaggaaat cttcagcggc ccactcgcct gccggaagcg ctgcgagcac
      361 gttgcgcatg agccgctcag agccggtgag cattgcgcct acgccctgaa gcaatcttgc
      421 atagccgaag cgttctacga gttggagcat gcgggtggcg ccgatgcggc atgcaccgag
      481 ctgcgcggtg aggtcgcctt cgcgttcttt cggagtgcgc acattggcga ggatggtcgc
      541 gagcatttgc gtgtccatct tgccggcgtg catcagcttc acgggaggaa tgcggatgcc
      601 ctcctgcgcg atctcgcgac aggggcccat ggagccggga tacatgccgc cgacatcggc
      661 gtggtgcgcg cggttcgcga caaagaacgc agcgcgattg cggcggggaa gaaatacagg
      721 cgccaccatg gtgatgtcgg gcaggtgcgt gccgccgcgg tagggatcat tgagcagcgc
      781 gacgtcgccg ggcgcgaggc gaagatctgc gagcacggcc gcaacagaca tgggcatgga
      841 accaaggtgg acgggcatgt cgtcgcccat cgcgatgagg tcgccatctg cggagaagac
      901 agccgaagag tagtcgcggc gctctttgat attgggcgaa aaggcggtgc ggcggagagc
      961 tgcacccatt tcgacggcaa cggaatgcag cgcgtgaccg atgacggcga tctcggcggg
     1021 atcgagttgt cggcgcgttt ttatgggcgg actcccgttg atggagcaag a
//
LOCUS       DASRVO010000443         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_350110, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000443 DASRVO010000000
VERSION     DASRVO010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350110"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFF05_05500"
     CDS             complement(<1..475)
                     /locus_tag="VFF05_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51291.1"
                     /translation="MKYLIGGLALAGLLAGAYGFWFAADSAHRELFEAAQATDGGRTP
                     GDADFNAIRLGYHGDPTDFRVEPKWYAQAAKQEARIARSMPRGAKTYARAANSPLALD
                     TNAFTLLGPQPLTGEGFGANRAAGRVNVAVSDPDDATVAWLGADGGGIWKTTNCCS"
     gene            complement(581..1516)
                     /locus_tag="VFF05_05505"
     CDS             complement(581..1516)
                     /locus_tag="VFF05_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW51292.1"
                     /translation="MQTLTVVVPAYNESAGLREFHARLAAVFDTLDLDCTALYVDDGS
                     RDDTWAVMESLRASDKRVATLKLSRNFGKELAMTAGLDAADADAVVVIDADLQDPPEL
                     IAQFVAAWREGFDVVYGTRSTRAGESSVKKFTSAAFYRVMERLSHTPVPRDTGDFRLL
                     SRRALDALKQVRERQRFMKGLYAWVGFRQKAIVYERDPRFAGSSKFNYWRLWNFAIEG
                     ITSFSTVPLRLATYVGVLTAFAAFVFGVFVFVKAVFYGDVVRGYPTLLLVILFLGGVQ
                     LMALGTIGEYLGRLYMEAKQRPLYLVEEQHPADSP"
BASE COUNT          329 a          565 c          551 g          313 t
ORIGIN      
        1 cggaacagca gttcgtcgtc ttccagatac cgccgccgtc cgcgccgagc cacgcgacgg
       61 tggcgtcgtc gggatcgctg accgccacat tgacgcgccc cgccgcgcgg tttgcgccga
      121 aaccctcgcc ggtcagcggc tgcgggccga gcaaggtgaa tgcgttggta tcgagggcaa
      181 gcgggctgtt cgccgccctg gcgtaggttt tggcgccgcg cggcatgctc ctggcgatgc
      241 gcgcctcctg cttcgccgct tgcgcgtacc atttcggctc gacgcgaaaa tcggtcgggt
      301 cgccgtggta gccaagacgg atcgcgttga agtcggcatc cccgggcgtg cggccgccgt
      361 cggttgcctg cgccgcctcg aacaattccc ggtgcgcgct gtccgcggca aaccagaatc
      421 catatgcgcc ggccagcagt ccggccagcg ccagaccgcc gatgaggtat ttcatgtgat
      481 tttcccgtga ctgaaagcgc ggattgcgta tccgccggac gacacttccc cgctggcgac
      541 tatagccgta tttccagccg ggaaaaacag cccgcgaacg tcatggcgaa tcggccggat
      601 gctgctcttc gaccaggtaa agcggccgct gcttggcttc catgtacagt cggcccaggt
      661 attcgccgat cgtgcccagc gccatcaatt gcacgccgcc gaggaacagg atgacgagca
      721 gcagcgtggg gtagccgcgc accacgtcgc catagaacac ggctttgacg aacacgaaca
      781 cgccgaacac gaaggcggcg aatgcggtca acacaccaac gtatgtcgcc agccgcaatg
      841 gcacggtgga aaaagaggtg atgccctcga tggcgaaatt ccacagtcgc cagtagttga
      901 atttgctgct accggcgaaa cgcggatcgc gctcgtagac gatggctttc tgccggaatc
      961 cgacccaggc atacaggcct ttcatgaagc gttggcgctc gcgcacctgc ttgagcgcat
     1021 ccagcgcgcg ccgactcagc aagcggaaat cgccggtatc gcgcggcacc ggcgtgtggc
     1081 tcaggcgttc catcacgcga tagaacgcgg cagacgtgaa tttcttcacg ctgctttcgc
     1141 cggcgcgcgt ggaacgggtt ccgtagacga catcgaatcc ttcgcgccac gcggcgacga
     1201 attgcgcaat cagttccggc ggatcctgca agtccgcgtc gatcacgacc acggcatcgg
     1261 cgtcggcggc atccagccca gcggtcatcg ccagttcctt gccgaagttg cggctcagtt
     1321 tcagcgttgc cacgcgcttg tcgctggcac gcaaggattc cataacagcc caggtgtcgt
     1381 cgcggctgcc gtcgtcgacg tacagcgcgg tgcaatccag atcgagcgtg tcgaatacgg
     1441 cggcaaggcg cgcgtggaat tcgcgcaggc ccgcgctttc gttgtaggcc ggaacgacga
     1501 cggtgagagt ctgcatggca cgcatgctaa cggaaagctg gtttccggtg aaaccgtctg
     1561 cgtgacaccg tacttgacaa gccgtcattt atttccataa ttccggaatt atgaaacaat
     1621 tagatgcaat cgccagtcta cgggcactgg cgcacgcgcg acgcctggag atcttccgcc
     1681 tgctcgtgca agccggcgcc ggtggcctga ccgtgggcac gctgcaatcg cgaacgcggc
     1741 acgccgcggc gacgctga
//
LOCUS       DASRVO010000444         1371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_142360, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000444 DASRVO010000000
VERSION     DASRVO010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142360"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VFF05_05510"
     CDS             <1..1184
                     /locus_tag="VFF05_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HZW51293.1"
                     /translation="LSGKPKEKESWWNLIRGVRGVLRQHYGHVALNFGEPIELTPLLD
                     AASPNWRNFSGDADVKPDWLNGLLDQVGEKILVNINRATDVNPVNLLALAILSTPKHA
                     MAEADLLDQLELVKGLLTAMPYSDRVTVTPLAPADIVAYGERMGWIGRIKHPLGDVLT
                     CEGPNAVLLSYFRNNVLHLIAAAAWVACCFLNNRRIAHETILRLGRMIYPFIQAELFL
                     PWDEDGFAERIGAIVEFFVGRGLLAHVDEGRILAREPGQSDSAFQLRTIAHSMLQAFE
                     RYYIAIAALVRNGPNTLTGPELENLCTLTAQRLSLLHELNAPEFFDKSLFRGFIQKLR
                     ERRVIWPDTNGRLEFDAALEDIVRDAKVILSREIRHGILKLTPERRNALAVPAEKPES
                     D"
     gene            complement(1181..>1371)
                     /locus_tag="VFF05_05515"
     CDS             complement(1181..>1371)
                     /locus_tag="VFF05_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HZW51294.1"
                     /translation="ADIARQLVELRIEHRDLDAAIARLADDVHCDELQLKRLKKRKLM
                     LKDAIARLESKLIPDLDA"
BASE COUNT          249 a          453 c          426 g          243 t
ORIGIN      
        1 aactgtccgg caagccgaag gaaaaggaat cctggtggaa cctgatccgc ggcgtacgtg
       61 gcgtgctgcg ccagcattac ggccacgtgg cgctgaattt cggcgaaccc atcgaactga
      121 cgcccttgct cgacgcggcg agcccgaact ggcgcaattt cagcggcgac gcggatgtga
      181 aacccgattg gctgaacggc ctcctcgacc aggtcgggga aaagatactg gtcaacatca
      241 accgcgccac cgacgtcaat ccggtcaatc tgctcgcgct cgcgatcctg tccacgccca
      301 agcatgcgat ggccgaggcg gacctgctcg accaactcga attggtcaag ggcctgctca
      361 ccgcgatgcc gtattcggat cgcgtcaccg tgacgccgct cgcaccggcc gacatcgtcg
      421 cctacggcga gcgcatgggc tggatcggcc gcatcaagca tccgctcggc gacgtgctga
      481 cgtgcgaagg ccccaacgcg gtgctgcttt cgtatttccg caacaacgtg ctgcacctga
      541 tcgcagcggc ggcgtgggtg gcgtgctgct tcctcaacaa ccggcgcatc gcgcacgaga
      601 ccatcctgcg cctgggtcgc atgatctatc cgttcatcca ggccgagctg ttcctgccct
      661 gggacgaaga cggcttcgcc gagcgcattg gcgccatcgt cgaattcttc gtcggccgtg
      721 gcctgcttgc gcacgtggac gagggccgca tcctcgcgcg cgagccgggc cagagcgaca
      781 gcgcgttcca gttgcgcacg atcgcgcaca gcatgctgca ggccttcgag cgctactaca
      841 tcgccatcgc ggcgctggtg cgcaacggcc cgaacacgct gaccggaccg gagctggaaa
      901 acctgtgcac gcttaccgcg cagcgcctgt cgctgctgca cgaattgaac gcgccggagt
      961 tcttcgacaa atccctgttc cgcggcttca ttcagaagtt gcgcgagcgc cgcgtgatct
     1021 ggccggatac gaacggacgc cttgaattcg atgccgcact ggaagacatc gtgcgcgacg
     1081 cgaaggtgat cctgtcgcgc gaaatccggc atggcatcct caagctcacg cccgagcgac
     1141 gcaatgcgct ggcggtgccg gcggaaaagc cggagtcgga ttaggcgtcc aggtccggaa
     1201 tcagcttgct ctccagccgc gcaatcgcat ccttgagcat caatttgcgc ttcttcaggc
     1261 gtttgagctg caattcgtcg caatgcacgt cgtccgcgag gcgggcgatg gccgcatcca
     1321 gatcgcggtg ctcgatgcgc agttcgacca gctgtcgggc gatgtccgcc g
//
LOCUS       DASRVO010000445         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_195349, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000445 DASRVO010000000
VERSION     DASRVO010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195349"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..>2093
                     /gene="topA"
                     /locus_tag="VFF05_05520"
     CDS             78..>2093
                     /gene="topA"
                     /locus_tag="VFF05_05520"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZW51295.1"
                     /translation="MAKNLLIVESPAKAKTINKYLGKDFQVLASYGHVRDLKPKEGAV
                     DTDRDFAMHYELIDKNEKHVDAIVKAAKSAQALYLATDLDREGEAISWHIAEILKQHK
                     LLDGKQVHRVVFSEITPHAIQDAVAHPRELSLDLVNAQQARRALDYLVGFNLSPVLWR
                     KVQRGLSAGRVQSPALRMICEREEEIEAFKAREYWTIEADCAHPVQRFAARLTRLRGK
                     KFEQFDLTNEKDAHAARDGLIKSAQGRLIVSEVQSKERKRRPAPPFTTSTLQQEAARK
                     LGFSTSRTMRIAQGLYEGVALGDEGTVGLITYMRTDSVSLANDAIAEIRKVIARDYGA
                     RALPEHPHLYRAKSKNAQEAHEAIRPTSALHTPASMAGFLNDDQRKLYDLIWKRAVAS
                     QMQHATLNTVSVDFDAGGDSTFRASGTTVVDPGFLAVYEEGRDTKADADSVNEREDEN
                     RKLPAMKVGEAIPLAQVLADQHFTEPPPRYSEASLVKTLEEYGIGRPSTYASIIQTLL
                     NREYVLLDSRRFRPTDVGRAVAKFLSGHFTQYVDYDFTAKLEDELDAVSRGEEDWVPL
                     LRKFWTPFKSLVAEKIETVDRGEAGGERVLGEDPASGKPVSVRLGRYGPMAQIGGRDD
                     ERKRFASLRPGQSIHTISLEDALHLFKLPRDLGQADDDETISVGIGRF"
BASE COUNT          431 a          700 c          645 g          317 t
ORIGIN      
        1 cgcggcatct gtgggcacac tgaaagaaga agggctcggc taatccgggc ccattgcatc
       61 tttaacggca cccccgcatg gccaaaaacc tgctcatcgt cgaatcgccc gccaaggcca
      121 agacgatcaa taaatacctt ggcaaggact tccaggtcct ggcctcctac ggccatgtgc
      181 gcgatctcaa gcccaaggag ggcgcggtcg acaccgaccg cgacttcgcc atgcactacg
      241 agctgatcga caagaacgag aagcacgtcg acgccatcgt caaggccgcc aagagcgcgc
      301 aagcgctgta tctggccacc gacttggacc gcgaaggcga ggcgatttcc tggcacatcg
      361 ccgaaatcct caagcagcac aagctgctcg acggcaaaca ggtgcaccgc gtggtattca
      421 gcgaaatcac gccgcacgcg atccaggatg cggtcgcgca tccgcgcgaa ttgtctctgg
      481 atctggtcaa cgcgcagcag gcgcgacgcg cgctcgatta cctggtcggc ttcaacctgt
      541 cgccggtgct gtggcgcaag gtgcagcgcg gcctctcggc cgggcgcgtg caatcgccgg
      601 cgctgcgcat gatctgcgag cgcgaagagg aaatcgaagc gttcaaggcg cgcgaatact
      661 ggacgatcga agccgactgc gcgcacccgg tgcagcgctt cgccgcgcgc ctgacccgcc
      721 tgcgcggcaa gaaattcgag cagttcgacc tcaccaacga aaaagacgcg cacgcagcgc
      781 gcgacggcct gatcaaatcc gcgcagggaa ggttgatcgt cagcgaggtg cagtccaagg
      841 agcgtaagcg ccgtcccgcg ccgccattca ccacctcgac gctgcagcag gaggccgcgc
      901 gcaagctggg gttctccacc agccgcacca tgcgcatcgc gcaaggtttg tacgaaggcg
      961 tcgcgctcgg cgacgaaggc acggtcggcc tgatcaccta catgcgtacc gattcggtga
     1021 gcctggccaa cgacgcgatc gcggaaatcc gcaaggtcat cgcgcgcgac tacggcgcgc
     1081 gcgcgctgcc cgaacacccg catctgtacc gtgccaaatc caagaacgcg caggaagcgc
     1141 acgaggcgat ccgcccgacc tcggcgctgc atacgccggc cagcatggcc ggcttcctca
     1201 atgacgacca gcgcaagctc tacgatctga tctggaagcg cgcggtcgcc agccagatgc
     1261 agcacgcgac gctgaacacg gtgtcggtcg acttcgatgc cggcggcgat tccacattcc
     1321 gcgccagcgg caccaccgtg gtcgacccag gctttctcgc cgtgtacgag gaaggccgcg
     1381 ataccaaggc cgacgccgat tcggtcaatg aacgcgagga cgagaaccgc aagcttcccg
     1441 cgatgaaagt cggcgaggcc atcccgctcg cgcaggtgct ggccgatcag catttcaccg
     1501 aaccgccgcc gcgttattcg gaagcctcgc tggtcaagac gctggaggaa tacggcatcg
     1561 gccggccgtc gacctacgcc agcatcatcc agacgctgct gaatcgcgaa tacgtgctgc
     1621 tcgattcgcg ccgtttccgt ccgaccgatg tcggccgcgc cgtggcgaaa ttcctgtccg
     1681 ggcatttcac ccagtacgtg gattacgatt tcaccgccaa actcgaggac gagctcgacg
     1741 cggtcagccg cggcgaagaa gactgggtgc cgctgctgcg caagttctgg acaccgttca
     1801 agtcgctggt cgcggaaaaa atcgaaaccg tggatcgcgg cgaagccggc ggcgagcgcg
     1861 tgcttggcga agatcccgcc tccggcaaac cggtgtccgt gcgcctgggc cgctatgggc
     1921 cgatggccca gatcggcggt cgcgacgacg agagaaaacg cttcgcctcg ttgcgcccag
     1981 gccagagcat ccatacgatt tcgctcgaag acgcgctgca cctgttcaag ttgccgcgcg
     2041 atctcggcca ggcggacgat gacgaaacca tcagcgtcgg catcggccgc ttc
//
LOCUS       DASRVO010000446         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_350255, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000446 DASRVO010000000
VERSION     DASRVO010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350255"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFF05_05525"
     CDS             <1..439
                     /locus_tag="VFF05_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003227292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZW51296.1"
                     /translation="ILALGRQNKMTGAAEQYQYILRDESMHCNFGIDLINQLKLENPQ
                     LWTAEFKADIKALFTHAVELEYRYAEDTMPRGVLGLNASMFKRYLRYIANRRATQIGL
                     EALFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGDALNWE"
     gene            566..1087
                     /locus_tag="VFF05_05530"
     CDS             566..1087
                     /locus_tag="VFF05_05530"
                     /inference="COORDINATES: protein motif:HMM:NF038053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like DNA-binding protein"
                     /protein_id="HZW51297.1"
                     /translation="MQGALHWLMFDQLEQEIAMATAKKSTAKKAAAKRPAAKKSAAKR
                     PAAKKAAAKRPAAKKAAAKRPAAKKSAAKRPAAKKAAAKRPAAKKAAAKRPAAKKAAK
                     KTTAKKTTAKKAAAKRPAAKKAAKKAPAKKAAKAPAAKPAASAAPAASAAPAAQTTLN
                     PQAAWPFPTASKP"
     gene            complement(1300..1917)
                     /locus_tag="VFF05_05535"
     CDS             complement(1300..1917)
                     /locus_tag="VFF05_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51298.1"
                     /translation="MFALAACGSRPPAPDWQMNAHDSLGRYLADFMAGDDPAAAAEFA
                     RARSDLASTGQPALVARAELTRCAALLANLVLDPCDGFERLREDAGPAERAYADYLGG
                     RLAPAEVDLLPAQHRVVARSGGAGALRSIADPLSRLVAAGVLFRTDRASPEVLTIGAD
                     TASAQGWRRPLLTWLGVQLRRAEAAGASQQVQRLRRRIALASGER"
     gene            complement(1944..>2317)
                     /locus_tag="VFF05_05540"
     CDS             complement(1944..>2317)
                     /locus_tag="VFF05_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51299.1"
                     /translation="FGNPADARKLIAALDRTNALLARVDALAGKADKQVFGTDGVVND
                     TRATVRELRQLLADTRASLTKVDAVLVEAQGIGANVRGATSDLGTLRGQVDANMRKIE
                     GMINELNRKWPLARDTQVKLP"
BASE COUNT          446 a          781 c          784 g          306 t
ORIGIN      
        1 gatcctcgcg ctcggacgcc agaacaagat gacgggggcc gccgagcagt accagtacat
       61 cctgcgcgac gagtcgatgc actgcaattt cggcatcgac ctgatcaacc agctcaagct
      121 cgaaaacccg caactgtgga cggcggaatt caaggcggac atcaaggcgc tgttcacgca
      181 cgccgtcgaa ctggagtacc gctatgccga ggacaccatg ccgcgtgggg tactgggcct
      241 caatgcttcc atgttcaagc gctatctgcg ttacattgcg aatcgtcgcg ccacgcagat
      301 cggcctcgaa gcgctcttcc cgaacgagga aaacccgttc ccctggatga gcgagatgat
      361 cgacctgaag aaggaacgca acttcttcga gacccgtgtg atcgagtacc agaccggcga
      421 tgcgctgaac tgggaatagg acgcatcggg ggcaggaact gcaccggacg ccgaccatgc
      481 cgcaaagagc atggcgccgt ccgggcaacg gtgttcatgg agctgggctt gcgcccaact
      541 tcatgaatcg ctccacgcaa ccaacatgca aggagcgctt cattggttga tgtttgatca
      601 acttgaacag gagatagcaa tggcaactgc gaagaaatcg acggcgaaga aggccgcggc
      661 gaagcgtccg gcagcgaaga agtcggcggc gaagcgtccg gcggccaaga aggctgccgc
      721 gaagcgcccg gccgcgaaga aggctgccgc gaagcgtccg gctgcgaaga agtccgccgc
      781 gaagcgtccg gccgcgaaga aggctgcggc gaagcgccca gccgcgaaga aagcggccgc
      841 gaagcgcccg gcagccaaga aggccgcgaa gaagacgacg gcgaaaaaga cgacggcgaa
      901 gaaggccgcc gcgaagcgcc cggccgcgaa gaaggcggcg aagaaggccc cggcgaagaa
      961 ggctgcgaaa gcgccggctg cgaaacccgc ggcaagtgca gcgcccgcgg catcggcagc
     1021 tcctgcggct cagaccactc tgaacccgca ggccgcgtgg cccttcccga cggcaagcaa
     1081 gccctgagac acagcagttc gtgtctgaaa gcccggcatc gaaaggtgcc gggctttttt
     1141 gttgcgcgtt cacgcgccgc ggcccgcaga atctgctcga tcaccttgcg cacgagccgc
     1201 cggcaacgcg cttccacaac gcttgtctcg ccagggtgac ctcgcggctg acacagcaga
     1261 tcgaaggcgg cgaacgtcga ccagcgagca ggtgcggtgc taccgctcgc cggacgcgag
     1321 cgcgatgcgg cggcgcaggc gctgcacttg ctggctggca ccggccgctt cggcgcggcg
     1381 caattggacg cccagccacg tgagcagcgg gcggcgccag ccctgtgccg aggccgtatc
     1441 ggcgccgatg gtgagcacct cggggcttgc gcggtcggtg cgaaagagaa caccggccgc
     1501 gaccagccgc gacagcggat cggcaatcga ccgcaaggcc ccggcgccgc ccgaacgcgc
     1561 gacgacccga tgctgcgccg gcaacaggtc gacctcggcc ggcgccagcc ggccgccgag
     1621 gtagtcggcg tacgcgcgct cggcgggccc cgcatcctcg cgcagccgtt cgaacccgtc
     1681 gcacggatcg agcacgagat tcgccagcaa tgcggcgcag cgcgtgagct cggcgcgggc
     1741 gaccagcgcc ggctggccgg tactcgccag atcgctgcgg gcccgcgcaa actccgcggc
     1801 cgccgccgga tcgtcgccag ccatgaaatc ggcgagatag cggcccaggc tgtcgtgcgc
     1861 gttcatctgc cagtccggcg cgggcggccg gctgccgcac gcggcgaggg cgaacatcag
     1921 cgcagccgcg agccacgcgc gcctcatggc agcttcacct gcgtgtcgcg tgcgagcggc
     1981 cacttgcgat tcagctcgtt gatcatcccc tcgatcttgc gcatgttcgc gtcgacctgc
     2041 ccgcgcaagg tcccgaggtc ggacgtggcg ccgcgcacgt tggcgccgat gccttgcgcc
     2101 tccaccagga cggcatccac cttggtcagg ctcgcccgcg tgtcggcgag cagttgccgc
     2161 aactcgcgca cggtggcacg ggtgtcgttc accacgccgt cggtcccgaa gacctgtttg
     2221 tcggccttgc ccgcgagggc gtcgacgcgg gcgagcaacg cattcgtgcg atcgagcgcg
     2281 gcgatcagct tgcgcgcatc cgcaggattg ccgaaca
//
LOCUS       DASRVO010000447         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_221285, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000447 DASRVO010000000
VERSION     DASRVO010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221285"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFF05_05545"
     CDS             <1..860
                     /locus_tag="VFF05_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009575640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZW51300.1"
                     /translation="DENRFVLALVRGDHEVNEVKLQKIAGMSEYRLANEAEILAALGA
                     KPGYLGPVHADRKKVHVIADRDVAAMADFVVGANKVDFHIAGVNWGRDLAEPDVVADI
                     RKAVEGDLSPDGKGVLKIARGIEVGHVFALGTKYSEAMHATVLDADGKNRPMQMGCYG
                     IGVSRIVAAAIEQNHDDAGIVWPEPMAPWRVAVCMINPKSDAKVNEAANALYAELNAK
                     GIETCLDDRGLRPGAMFADIELIGIPHRVVVSERGLAAGTFEYRARRDTESRNLSREE
                     LFALLECTR"
     gene            complement(930..1838)
                     /locus_tag="VFF05_05550"
     CDS             complement(930..1838)
                     /locus_tag="VFF05_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51301.1"
                     /translation="MQAARSGAVPGGPSVGLLDELEQQAQQRKASADDAEKRKSQREE
                     IFRTQLEPGMNALHDFLGKLVANLKLLQPKKQLRFALIGYGEIVGYIEHEYDLKSNQQ
                     PGSREIVLSFPCLVASEECPTVEVVGASKVRTVAGAFQRYHLGGLLEPKKDGNGDVIS
                     AKFNAKGRIPMTATFAADADGAVVRASFVNFDALGTATKNIPSAQLNESTFDAIGRYL
                     MREESNLFQEALSDSFRAQLRTKVQQDQIKRRWESKIGAQQKAELEQLKREQSLTGRF
                     AKPVIKESKPAAGPSWIDKVKGLIKK"
     gene            1995..2312
                     /locus_tag="VFF05_05555"
     CDS             1995..2312
                     /locus_tag="VFF05_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HZW51302.1"
                     /translation="MAIELKNLNKKQLAELIARASSRSRELDAAELDKVRADVRAYAK
                     TKGYTIEQLFGGRGRKGKRSKAAPKYRNPADPSQTWSGRGKRPRWFHAALKAGMREKD
                     LLI"
     gene            complement(2365..>3115)
                     /locus_tag="VFF05_05560"
     CDS             complement(2365..>3115)
                     /locus_tag="VFF05_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HZW51303.1"
                     /translation="ALASEGRPRVAASFEAVSALLATLIATAVGTWLVALNVKAVVLA
                     ALIVLLPGLSLTTAVRELSTQHLASGVARLAGAMMSLLKLAFGTLAATQIAVALHWVP
                     KVVAPGAVPPWAQWVALGFGCLSFAVLFQAARRDYVLVMASAALGYVVAYYGSSAFSP
                     NFGVFLAGLVIGAASNAYARVAHRPGALVREPGIILLVPGSVGFRTLSLMFERNVYTG
                     LDVGATLISILVALVAGLLFGDLLVAPRRSL"
BASE COUNT          617 a          990 c          969 g          539 t
ORIGIN      
        1 gcgatgagaa tcgattcgtg ctcgcactgg tgcgcggcga tcacgaggtc aacgaggtca
       61 agctgcagaa gatcgctggc atgtccgaat accgccttgc gaacgaagcc gaaatcctcg
      121 ccgcgctcgg cgcaaaaccg ggttatctcg ggcccgtgca tgcggatcgc aagaaagttc
      181 atgtcatcgc cgaccgcgac gtcgccgcaa tggccgattt cgtcgtcggc gcgaacaagg
      241 tcgatttcca catcgccggc gtcaactggg gccgcgacct ggccgagccc gatgtcgtcg
      301 ccgacatccg caaggcggtc gaaggcgatt tgtcgccgga tgggaaaggc gtgctgaaga
      361 tcgcgcgtgg catcgaggtc ggccacgtgt tcgcgctcgg cacgaagtac tccgaggcga
      421 tgcacgcgac cgtgctcgac gccgacggca agaatcgccc gatgcagatg ggctgctacg
      481 gcatcggcgt gtcgcgcatc gtcgccgcgg cgatcgagca gaaccacgac gacgccggca
      541 tcgtctggcc ggaaccgatg gcgccgtggc gcgtcgcggt atgcatgatc aatccgaaga
      601 gcgacgccaa ggtgaacgaa gcggcgaacg cgctgtacgc cgaactcaac gcgaaaggca
      661 tcgaaacctg cctcgacgac cgcggcctgc gccctggcgc gatgttcgcc gacatcgagc
      721 tgatcggcat ccctcatcgg gtggtggtgt ccgaacgcgg actcgccgcc ggcacgttcg
      781 aataccgtgc gcgacgcgac accgagagcc ggaatctttc gcgcgaagaa ttgttcgcgc
      841 tgttggagtg tacacgttag tacacgagca ttccgagcac cgccggaacg ctagatgtcg
      901 agcacagcgg cttgttcagg gctctagcgc tacttcttga tcaaaccctt gactttgtcg
      961 atccatgatg gaccggcggc cggctttgat tccttgatga ccggtttggc gaaacggccg
     1021 gtcaggcttt gttcgcgctt gagctgttcg agctccgctt tctgctgcgc gccgatcttg
     1081 gactcccagc gccgcttgat ctgatcctgc tgcaccttgg tgcgcagttg cgcgcggaac
     1141 gaatccgaca gcgcttcctg gaacaggttg ctctcctcgc gcatcagata gcggccgatg
     1201 gcgtcgaacg tggattcgtt gagctgtgcg gacggaatgt tcttggtcgc cgtaccgagc
     1261 gcatcgaaat tcacgaagct cgcacgcacc accgcgccgt cggcgtcggc agcaaacgtg
     1321 gccgtcatcg gtatccgacc cttggcattg aatttcgccg agatcacgtc gccattgccg
     1381 tcttttttcg gctccagcaa gccgcccagg tgatagcgct ggaacgcgcc ggctacggtc
     1441 ctgaccttgc tcgcaccgac cacctcgacc gtggggcatt cctcgctcgc caccaggcac
     1501 gggaaactca gcacgatttc gcgcgaaccc ggctgctggt tggacttgag gtcgtattcg
     1561 tgttcgatgt agccgacgat ctcgccgtag ccgatcaggg cgaagcgcaa ctgtttcttc
     1621 ggttgcagaa gcttgagatt ggcgacgagc ttgccaagga aatcgtgcag cgcgttcatg
     1681 cccggctcga gctgggtgcg aaaaatctcc tcgcgctgcg acttgcgctt ttccgcgtcg
     1741 tcggcgctgg ccttgcgctg ctgcgcctgc tgttcgagct cgtcgagcaa tcccacggat
     1801 gggccccctg gcaccgcgcc ggaacgcgcg gcctgcatga tgattaactt aattccaacg
     1861 gggcgagcat gcaagcatcg cgtaacccgc aagggcggag aatgtgacgc tcgccgcgga
     1921 ttttttgatt tgcggtcggg ttaatggcat tctatcggcg cgattttaac cacttctaat
     1981 tgcgagagtc gtcgatggca atcgaattga aaaatctcaa caagaaacaa ctcgcggaat
     2041 tgatcgcacg cgcgtcgtcg cgcagccgcg aactggatgc cgcggaattg gacaaggtcc
     2101 gtgccgacgt gcgcgcctac gccaagacga aaggctatac gatcgagcaa ttgttcggtg
     2161 ggcgcgggcg caagggcaag cgcagcaagg ccgcgccgaa ataccgcaac ccggccgatc
     2221 catcgcaaac ctggtccggc cgcggcaagc ggccgcgctg gttccacgcc gcactcaagg
     2281 ctggcatgcg cgaaaaggat ttgcttatct gaccgcgaat tgctgcgcga aaaaatgccg
     2341 gcttttgccg gcattttttt tgcgtcacag actgcgtcgc ggggcgacca aaagatcgcc
     2401 gaacaataga ccagcgacga gtgcgaccag aatcgagatc aaggtagcgc ccacgtccag
     2461 ccccgtgtag acattgcgct cgaacatcag cgaaagggtg cggaaaccca cgctgcccgg
     2521 caccagcagg atgatgcccg gctcgcgcac cagcgccccg ggtcgatgcg ccacgcgcgc
     2581 gtaggcattg ctcgccgcgc caatcaccag accggcgagg aacacgccga aattcggcga
     2641 aaatgccgag ctgccgtaat acgccacgac gtagccgagc gccgccgaag ccatgaccaa
     2701 cacgtagtcg cggcgtgccg cctggaacag cacggcgaag gacaggcaac caaagcccag
     2761 cgcgacccac tgcgcccacg gcggcaccgc acctggagcc acgactttgg gcacccaatg
     2821 cagggcgacc gctatctggg tcgcggcgag ggtgccgaaa gcgagcttga gcaagctcat
     2881 catcgcgccg gccaggcgcg ccacacccga ggcgaggtgc tgggtcgaca gctcgcgcac
     2941 cgcggtggtc agactcaatc ccggcaacag cacgatcagc gccgcgagta ccactgcctt
     3001 gacgttcaat gcgaccagcc acgtgccgac ggcagtggcg atcaaggtgg ccaacaaggc
     3061 cgacactgcc tcgaaactgg ccgccacccg cggcctgccc tcgctggcca gcgcg
//
LOCUS       DASRVO010000448         1352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_273405, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000448 DASRVO010000000
VERSION     DASRVO010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273405"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VFF05_05565"
     CDS             <1..798
                     /locus_tag="VFF05_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HZW51304.1"
                     /translation="GAAHQRALVDLASQFRDFGGDAVIVADLALISELREHVPDLAVH
                     VSSVAACRNSAGVGFYRDMGVRRVILPRDVTLDEIGAIAKAAGDIEVEVFVLNDGCVF
                     EEGACHSIHLPAKQGGPICIDSRPFSYRDAKGGTISARTARMLRENDADYQRWLWYRF
                     GCGFSTTAESYPFGPCGLCAISRLQDAGVTSVKIAGREGATARKLKSVEMVRAVVQRV
                     HGGSPQEDVARFAVGIRKSEQHCRAGFMCYYPEVRWKAERAEEAPSG"
     gene            1035..1169
                     /locus_tag="VFF05_05570"
     CDS             1035..1169
                     /locus_tag="VFF05_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51305.1"
                     /translation="MADALSELIDRCCTADDNMSCPLIARLQFESEDSSPRCSRRAGG
                     "
BASE COUNT          237 a          425 c          458 g          232 t
ORIGIN      
        1 ggcgccgcgc accagcgtgc gctggtggac ctggccagcc aattccgcga tttcggcggc
       61 gatgccgtga tcgtcgcaga tctcgccctg atctcggaac tgcgcgaaca cgtgccggac
      121 ctggccgtcc acgtaagctc ggtcgccgct tgccgcaaca gcgccggcgt cggtttctac
      181 cgggacatgg gcgtcaggcg cgtgattctt ccgcgcgacg tcacgctcga tgagatcggc
      241 gcgatcgcga aggcggccgg cgatatcgag gtggaggtat tcgtcctcaa cgatggctgc
      301 gtgttcgagg agggcgcctg ccactcgatc cacttgccgg ccaagcaggg agggccgatt
      361 tgcatcgaca gccggccctt cagctaccgc gacgcgaaag gtggcacgat ctcggcgaga
      421 accgcgcgga tgctgcgcga gaacgacgcg gactaccagc gctggctgtg gtatcgattc
      481 ggctgcggat tttcgaccac cgccgaaagc tatccgttcg gtccctgcgg actgtgcgcg
      541 atttcgcgtc tgcaggacgc aggcgtcacc tcggtaaaga tcgcgggccg cgagggcgct
      601 accgcgcgaa agctcaagag cgtcgaaatg gtgcgcgcgg tggtgcaacg ggtgcacggc
      661 ggcagcccgc aagaggacgt ggccaggttt gcagtgggta tccgcaagag cgagcagcat
      721 tgccgcgccg gattcatgtg ctattacccg gaggtgcgct ggaaagcgga acgcgcagag
      781 gaggctccaa gcggctgacc cgcagtgatc aaagaactgc cccggtgcag agatgtcacg
      841 cgcatcgcct gcgacgaacg cacgccgcgg cggcaagccg gttcgggcta cgcactacgc
      901 gccttgtcgc acagcggaag gacttacacc gctttccggg acatcaacgt gggcgatgtc
      961 tgcagtacct tgggacctgt ggttcgtgcc gcgacgcttt cgacgtccgc cgcaagctcg
     1021 aagacctgca gcggatggcc gatgcgctca gcgaactgat tgaccggtgc tgcacggccg
     1081 acgacaacat gagctgcccg ctgatcgccc gccttcaatt cgagagcgaa gactcttcac
     1141 cacgttgcag ccggcgcgcc ggcggctgac cgatcttgcg gatgaaggcg cgcgtcaatg
     1201 cgccgggtac tatcgcagcc gacgtccgcc ggatcggtgc ggtgggttgg ctgtggcatc
     1261 gtgtccaggc ttcaactttc attcctgggc tcctccagat ccggcgtgcc cgagaacaga
     1321 tcgccgctgg cggcatgaat gccgctcagt tg
//
LOCUS       DASRVO010000449         1433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_168098, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000449 DASRVO010000000
VERSION     DASRVO010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168098"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFF05_05575"
     CDS             <1..584
                     /locus_tag="VFF05_05575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZW51306.1"
                     /translation="AARLREETAKLPQAGMQIGPDQAAFLALLVRAIGAKRCLEVGTF
                     TGMSGLAVASALPDDGKLVCCDISEEWTSIARRYWNEAGVDARIDLRLAPALDTLRDL
                     AKTQRGRFDFAFIDADKSGYDAYYEACLELLRRGGLVALDNMLWSGKVADANAHDADT
                     DAIRALNAKIHADQRVDATLITLGDGVMLARKR"
     gene            complement(585..1304)
                     /locus_tag="VFF05_05580"
     CDS             complement(585..1304)
                     /locus_tag="VFF05_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51307.1"
                     /translation="MIFRRAIALAAVLMLAACSGNDAPPVDPAVAAKAAAEARAAKEG
                     ALYDEMRQSGSWDIAVSLGNEILAKYAGTQTAARVQQTIAETREKSAAIADSHRLARL
                     WTYTATAEDGGTQYAAAIATKEPLKIANARLRLVLRQHPKWGQSVYLLLDSATFDCKG
                     GCATLPVSFDGAKAQRMQATIPPTGEPALFIDDDKGFIAKLEKAKIVAIGANVKGLGE
                     TTFKFEVGGFDAARLPARPRK"
BASE COUNT          235 a          473 c          464 g          261 t
ORIGIN      
        1 tcgccgcgcg cctgcgcgag gaaaccgcga aactgccgca ggccggcatg cagatcgggc
       61 ccgaccaggc cgcgttcctc gccctgctcg tgcgcgcgat cggcgcgaag cgctgcctcg
      121 aggtcggcac attcaccggc atgagcgggt tggcggtcgc gtccgccctg cccgacgatg
      181 gcaagctcgt gtgctgcgac atcagcgaag aatggacgtc catcgcgcgg cgctactgga
      241 atgaggcggg cgtcgacgcg cgcatcgacc tgcgcctggc gccagcgctg gacacgctgc
      301 gcgatctcgc caagacccag cgcggccgct tcgacttcgc cttcatcgac gcggacaagt
      361 ccggctacga tgcctattac gaagcctgcc ttgaactgct gcgccgcggc ggactcgttg
      421 cgctcgacaa catgctctgg tcgggaaagg tcgccgatgc gaacgcgcac gatgccgaca
      481 ccgacgcaat ccgcgcgctg aatgcgaaga tccacgccga tcagcgcgtc gatgcaacgc
      541 tgatcacatt gggcgatggc gtgatgctgg cgcgcaagcg ctgatcactt gcgcggcctt
      601 gccggcaacc tggctgcatc gaatccgccg acttcgaact tgaacgtcgt ttcacccaac
      661 cctttcacgt ttgcgccgat ggcgacgatt ttcgctttct cgagtttggc gatgaaaccc
      721 ttgtcgtcgt cgatgaacag ggccggctcg ccggtcggcg ggatcgtcgc ctgcatgcgc
      781 tgcgccttgg cgccgtcgaa actcaccggc agcgtcgcgc aaccgccctt gcagtcgaac
      841 gtggcgctgt caagcagcag gtacacgctc tggccccatt tcgggtgctg gcgcaagacc
      901 aggcgcaggc gtgcgttcgc gatcttcaac ggttccttgg tcgcgatcgc cgcggcgtac
      961 tgcgtgccgc cgtcctcggc cgtggcggtg taggtccaca ggcgcgcgag gcgatgcgag
     1021 tcggcgatgg cggcggattt ctcgcgtgtt tcggcaatgg tctgttgcac gcgcgccgcc
     1081 gtttgcgtac ccgcatactt ggcaaggatt tcgttgccaa gcgacacggc aatatcccaa
     1141 ctgccgcttt ggcgcatctc gtcgtagagc gcgccttcct tggcggcccg cgcctcggca
     1201 gcggctttcg ccgcgacggc gggatcgacg ggtggcgcat cgtttcccga acaggcggcc
     1261 agcatcaaca cggcggcgag tgcgatggcg cggcgaaaaa tcatggaacg atctcgttat
     1321 cggcgatcgc gccaagatac cggatttgcc gctcacgcgc agggatcgcg acccaggttt
     1381 ttatcgtagg tggaaacctg ttcgtcgaac tcgtgaccgc cacgaccgat aga
//
LOCUS       DASRVO010000450         1107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_350323, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000450 DASRVO010000000
VERSION     DASRVO010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350323"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFF05_05585"
     CDS             <1..442
                     /locus_tag="VFF05_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51308.1"
                     /translation="ILASGDDAISEPAEQAIESTLMHRGYRVVDQDSLPRVGRLIDGE
                     RPRFAEALAVLQGHADAVVFVHARKVGSQEMNYYGQTSTLYTAQLNVRAYSVEGRRQL
                     GPPWSEQVNFTSLNAGEKAKEAVEPMLERVSERLVDFRPKGRRE"
     gene            complement(479..>1107)
                     /locus_tag="VFF05_05590"
     CDS             complement(479..>1107)
                     /locus_tag="VFF05_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZW51309.1"
                     /translation="DYTLPFVALVAGRRVARLLREAFGPRDITDLALPYYCITSNLTA
                     GRAETHRAGPLWFWLRASCAIPGVLPPVFHRGEVFVDGAVMDNLPVDAMHAQGVAEII
                     AVDIGADDVLHAEVEEFALPPWWKLLWQRWFHRHQRPGILSILLRSGMVNAEAASLER
                     RARTSLLLTPPLNDVELLDWKAYERAIDAGYSYAQKLLGRANAPRELP"
BASE COUNT          203 a          355 c          369 g          180 t
ORIGIN      
        1 catcctcgcc agcggcgacg atgcgatttc ggagccggcc gaacaagcca tcgaatccac
       61 gctgatgcac cgcggctatc gcgttgtcga ccaggattct ctgccacgcg tgggccgctt
      121 gatcgacggt gagcgcccgc gtttcgccga agcactggcc gtgctgcagg gccatgccga
      181 tgccgtggtg ttcgtgcacg cgcgcaaggt cggctcgcag gagatgaact actacggcca
      241 gacctccacg ctgtacaccg cccaactcaa cgtgcgcgcc tactcggtcg aaggcaggcg
      301 tcaactcggg ccgccgtgga gcgagcaggt gaatttcacc agcttgaacg ccggcgagaa
      361 agccaaggaa gcggtcgagc cgatgcttga gcgcgtcagc gaacggctgg tggacttccg
      421 gcccaaggga cgccgcgaat aagtcgagaa cctcggtacg ctttgccgta ccgaggtttc
      481 agggcagttc gcgtggcgcg ttcgcccgtc cgagtaattt ctgcgcgtag ctgtaacccg
      541 catcgatcgc gcgttcgtag gctttccagt cgagcagttc gacgtcgttc aacggcggcg
      601 tcaacaacag actcgtgcgc gcgcgtcgct ccaggctcgc cgcttccgcg ttgaccatgc
      661 ccgaacgcaa caggatgctg aggatgccgg gccgctggtg gcgatggaac cagcgctgcc
      721 aaagcagttt ccaccacggc ggcaaggcga attcctcgac ttcggcatgc agtacgtcgt
      781 cggcgccgat atccacggcg atgatttcgg ccacgccttg ggcgtgcatc gcgtcgaccg
      841 gcaggttgtc catcaccgcg ccgtcgacga acacttcgcc gcgatggaac accggtggca
      901 ggacaccggg aatcgcgcaa ctcgcgcgca gccagaacca cagcggcccg gcgcggtggg
      961 tttcggcacg gcccgcggtg agattggagg tgatgcagta gtagggcaag gccagatcgg
     1021 tgatgtcacg cgggccgaac gcttcgcgta gcaggcgcgc gacgcggcgc ccggcgacga
     1081 gcgcgacgaa cggcaacgtg tagtcgc
//
LOCUS       DASRVO010000451         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_168140, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000451 DASRVO010000000
VERSION     DASRVO010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168140"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VFF05_05595"
     CDS             complement(<1..1307)
                     /locus_tag="VFF05_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZW51310.1"
                     /translation="MIEVDYSRQARHWIAGEWVDDGASRHFESIAPATGEPLGTGPCG
                     RQLDAVAAIAAARRAFESTPWRHSPRIRAAALLAMADRLEADRQPLAALLNAENGKVL
                     RECVGEVDGAASELRYYAGVARLGAGRVVEPAPGIRSLVTHEAAGVAAIIVPWNAPLI
                     LFVRSLAPALAAGCTVVVKAAPQSALFMQRVAERLAEVTATPAGTVNMVFENGHEVAQ
                     EFVRSAAVDVLSYTGSTAVGKRIMADAAQTLKRLSLELGGKAPCVVCDDADLGLAVPQ
                     ILAAGTILSGQQCTAATRILVQRGILPAFKEAMTGAMRAFRVGPGHLPDSDMGAVIDL
                     ANRDRILRLIDHTAERHRIVVRGGKVEGLAPNGAFVSPTLVEVDDPRSDVVQLEHFAP
                     LMTLEVFGNDRDGVALANATRFGLGSSVWSRDHARAQRMAREIR"
     gene            complement(1304..>1556)
                     /locus_tag="VFF05_05600"
     CDS             complement(1304..>1556)
                     /locus_tag="VFF05_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51311.1"
                     /translation="AAVVGVPDEVYGEAVAAFIELKPGCELGVREVLEHSKSHIASYK
                     KPKYVFFVDSFPRNAMGKVLKIELRARALERLSAQGVAG"
BASE COUNT          253 a          564 c          492 g          247 t
ORIGIN      
        1 cggatttcgc gggccatgcg ctgggcccgc gcatggtcgc gcgaccagac gctggagccg
       61 aggccgaaac gggtggcgtt cgccaatgcg acaccgtcgc ggtcgttccc gaaaacctcg
      121 agcgtcatca gcggcgcgaa atgctcgagt tgcacgacat cgctgcgcgg atcgtccacc
      181 tcgaccaacg tcggcgatac gaaggcgccg ttcggcgcca ggccctcgac ttttcctccg
      241 cgcaccacga tgcggtgccg ttcggccgtg tggtcgatca ggcgcaggat gcgatcgcgg
      301 ttggccaggt cgatcaccgc gcccatgtcg ctgtccggca ggtggccggg gccgacccga
      361 aatgcccgca tcgccccggt catcgcctcc ttgaacgcag gaaggatgcc gcgctgcacc
      421 agaatgcgcg tcgccgccgt gcattgctgg cccgaaagaa tcgtcccggc cgccaggatc
      481 tggggcaccg ccaaacccag gtcagcgtcg tcgcaaacga cgcagggcgc tttgcctccc
      541 aattcgagcg agaggcgctt gagcgtctgc gccgcgtcgg ccatgatgcg cttgcccacc
      601 gccgtgctgc cggtgtagct gagcacgtcc actgcggcgg agcgcacgaa ttcctgcgcc
      661 acttcatgcc cgttctcgaa caccatgttc accgttccgg ccggcgtcgc cgtgacttcc
      721 gccaggcgtt cagccacacg ctgcatgaac agcgcgcttt gcggggccgc tttcaccaca
      781 accgtgcagc ccgccgccag ggccggcgcc agcgacctca cgaacaggat cagcggtgcg
      841 ttccacggca cgatgattgc ggcgacgccc gccgcttcgt gagtgaccag cgaacggatg
      901 ccgggcgcgg gctcgaccac ccggccggcg cccaggcgag ccactcctgc gtagtagcgc
      961 aactcggagg cagcgccgtc gacctcgccc acgcattcgc gcagcacctt gccgttttcg
     1021 gcgttcagca gcgccgccag cggctggcga tcggcttcca ggcggtctgc catggccagc
     1081 aacgctgccg cgcgaatgcg tgggctgtgg cgccaaggcg tcgattcgaa agcccgacgg
     1141 gccgctgcaa tcgccgcaac ggcatccagc tgccgcccgc aagggcccgt gccgagcggc
     1201 tcgccggtag ccggggcaat cgactcgaag tggcgggaag caccgtcgtc gacccattcc
     1261 ccggcgatcc agtggcgtgc ctggcgcgag taatcgactt cgatcatcct gccacccctt
     1321 gcgcactgag ccgttcgagc gcgcgagcgc gcaattcgat cttcaggacc ttgcccatcg
     1381 cgttgcgcgg aaatgaatcc acgaagaaca cgtatttggg tttcttgtaa ctcgcgatgt
     1441 gcgacttgct gtgttccagc acctcgcgca cgcccagctc gcacccgggc ttcagctcga
     1501 tgaaggccgc caccgcctcg ccgtagacct cgtccggaac accgaccacc gccgca
//
LOCUS       DASRVO010000452         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_325306, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000452 DASRVO010000000
VERSION     DASRVO010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325306"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..501
                     /locus_tag="VFF05_05605"
     CDS             70..501
                     /locus_tag="VFF05_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51312.1"
                     /translation="MPTPMLLLTLLCGLTAILLVCVRRIPEGQAYTLRRVDGHMRTVG
                     AGVHVVLPLIERVAHKIRLFGNVVDIAAPAADGDLRGRVYFQVLDAKRADAVIDGIAD
                     LLRARVPTLATQGAGDAASLKVELNRELNPRGVLVTRVQLG"
     gene            579..1235
                     /gene="rsmG"
                     /locus_tag="VFF05_05610"
     CDS             579..1235
                     /gene="rsmG"
                     /locus_tag="VFF05_05610"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZW51313.1"
                     /translation="MSISDRASLYARLQAGLAELGLAPGADACERLLDYVELLTRWNA
                     AYNLTAVRAPDEMVTRHLLDSLAVAKLVRGDSLADLGTGAGLPGIPLAILAPERQHTL
                     IDSNGKKVRFLREAVRTLGLANVQVQQSRVEDARGQYDCITARAFATLGEMLRLGGHL
                     LAPDGIWIALKGRLSKEEILGVPAGFAVADVMVLTVPGLGAARQVAIIKRTNAQEQAA
                     "
     gene            1232..2035
                     /locus_tag="VFF05_05615"
     CDS             1232..2035
                     /locus_tag="VFF05_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZW51314.1"
                     /translation="MTRIIAIANQKGGVGKTTTAVNLAAALAETRRKILLIDFDPQGN
                     ATMASGIDKRIAKPSGCEVLLEEASIESAIVPTPAGFDLLPGNRDLTAAELKLMDALA
                     RESRLKEQIAKLGERYHTVLIDCPPSLHLLTINAFTAAHSVLIPIQCEYFALEGLTSL
                     LETIKAVRARLNPHLEIEGLLRTMYDVRNNLANDVSAQLTKHFGDKLLRSIIPRNIRL
                     AEAPSHGQPINLYDRESRGAIAYLGLAGEMIRRERGGAVAHVGPAHATN"
     gene            2046..>2663
                     /locus_tag="VFF05_05620"
     CDS             2046..>2663
                     /locus_tag="VFF05_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZW51315.1"
                     /translation="MAAAKRRGLGARDLDVLLGGPEADSGPEAAGEYRSLKIGSLQAG
                     KYQPRKAFAQEPLDELVASIREHGVLDPILVRAIGAQKFEIIGGERRWRAAKQAGLAE
                     IPALVRELTDSQVALIALVENIQREDLAPLEVAQSLKQLMAEFKFTQEQAADKIGRSR
                     AAVANLLRLLGLPETVKQLLAARRIDMGHARALVTLEPKLASQLAK"
BASE COUNT          469 a          870 c          882 g          442 t
ORIGIN      
        1 atactggctt gcattcttga acgtttttcg aataaaatac gttaacctct tgataaagag
       61 gatataaaaa tgcctacccc gatgctgttg ttgaccctgc tctgtggcct gaccgccatc
      121 ctgctggtct gcgtgcgtcg cattccggaa ggtcaggcct acaccctgcg ccgcgtcgac
      181 ggacacatgc gcaccgtcgg cgccggcgtg cacgtggtgc tgccgctgat cgagcgcgtc
      241 gcgcacaaga tccgcctgtt cggcaatgtg gtcgatatcg ccgcgcccgc agcggacggc
      301 gacctgcgtg gccgcgtgta tttccaggtg ctcgacgcca agcgcgccga cgccgtgatc
      361 gacggcatcg ccgacctgtt gcgcgcacgc gtgccgacgc tggccacgca aggcgcgggc
      421 gatgccgcgt cgctgaaggt cgagctcaac cgcgaattga acccgcgcgg cgttctggtc
      481 acgcgggtgc agttgggata attccgccgc gccggttgcg ctgaatcgat tgcgcacgga
      541 tacttgcgag ccctgcgcat tgcgcgcggg gcttttttat gagcatttcc gaccgcgcca
      601 gcctctatgc ccgcctgcag gcgggactcg ccgaactggg cctggcgccc ggcgcggacg
      661 cctgcgagcg cctgctcgat tacgtcgaat tgctgacgcg ctggaatgcg gcctacaacc
      721 tcaccgcggt gcgcgcgccg gacgagatgg tcacgcgcca tttgctcgat tcgctggccg
      781 tggcgaaact tgtccgtggc gacagcttgg ccgacctcgg taccggcgcg ggcttgcccg
      841 gcataccgct tgccatcctc gcgcccgagc gccagcacac gctgatcgac agcaacggca
      901 agaaggtgcg tttcctgcgt gaagccgtgc gtacgctggg ccttgcgaac gtgcaggtgc
      961 agcagtcgcg cgtggaagat gcgcgtggcc aatacgattg catcaccgcg cgcgccttcg
     1021 ccacgcttgg cgagatgttg cgcctgggcg gacatctgct ggcaccggac ggcatctgga
     1081 tcgcgctcaa gggccggttg tcgaaggaag aaatcctcgg cgtgcctgcc gggttcgccg
     1141 tcgccgacgt gatggtgctg accgttccgg gcttgggcgc ggcgcgccag gtggcgataa
     1201 tcaaacggac gaacgcacag gagcaggcgg cgtgacgcgc atcatcgcca ttgccaatca
     1261 gaaaggcggc gtcggcaaga cgaccacggc ggtgaatctc gccgccgcgc tggccgagac
     1321 gcggcgcaag atcctgctga tcgacttcga tccgcagggc aacgcgacga tggcctccgg
     1381 catcgacaag cgcatcgcca agccgagcgg ctgcgaagtg ctgctcgagg aagccagcat
     1441 cgaatcggcg atcgtaccga cgccggcggg attcgatctt ttgcccggca accgcgacct
     1501 cactgccgcg gaactgaaac tcatggatgc gctggcgcgc gaatcgcgcc tgaaggaaca
     1561 aatcgccaag ctcggcgagc gctaccacac ggtgctgatc gactgtccgc cgtcgctgca
     1621 cctgctcacg atcaacgcct tcaccgccgc gcacagcgtg ctgattccga tccaatgcga
     1681 atacttcgcg ctggaaggat tgaccagcct gctcgagacg atcaaggcgg tgcgcgcgcg
     1741 cctgaatccg catctggaaa tcgaaggcct gttgcgcacc atgtacgacg tgcgcaacaa
     1801 cctcgcgaac gacgtttcgg cgcagttgac caagcacttc ggcgacaagc tgctgcgctc
     1861 gatcatcccg cgcaacatcc gcctggccga ggcgccgagc cacggccagc cgatcaacct
     1921 gtacgaccgc gaatcgcgcg gcgcgatcgc ctatctgggc cttgccggcg agatgatccg
     1981 gcgcgaacgc ggcggcgcgg tggcgcacgt cggccccgcg cacgcaacga actgaggatg
     2041 cactgatggc ggcagcaaaa cggcgcggac tcggagcgcg cgatctggat gtcctgctcg
     2101 gtgggccgga ggccgattcg ggtcccgaag ccgccggcga gtaccgctcg ctcaagatcg
     2161 gcagcctgca agccggcaaa taccagccgc gcaaggcgtt cgcgcaggaa ccgctggacg
     2221 aactcgtcgc ttcgatccgc gagcacggcg tgctcgatcc gatcttggtg cgcgctatcg
     2281 gcgcgcagaa gttcgagatc atcggcggcg aacggcgctg gcgcgcggcg aagcaggcgg
     2341 gtctggccga gattcccgcg ctggtgcgcg agttgaccga ttcgcaggtt gcgctgatcg
     2401 cgctggtcga gaacatccag cgcgaggact tggcgccgtt ggaagtggcg cagtcgctca
     2461 agcagctcat ggccgagttc aagttcaccc aggagcaggc cgcggacaag atcggccgtt
     2521 cgcgtgcggc cgtggcgaac ctgctgcgcc tgctgggcct gcccgagacg gtgaaacagc
     2581 ttctggccgc gcgccggatc gacatgggcc atgcgcgcgc gctggtcacg ctggaaccga
     2641 aactggcgtc gcaactggct aaa
//
LOCUS       DASRVO010000453         5848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_195470, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000453 DASRVO010000000
VERSION     DASRVO010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195470"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1943)
                     /locus_tag="VFF05_05625"
     CDS             complement(111..1943)
                     /locus_tag="VFF05_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51316.1"
                     /translation="MKTPCGWRLALPLVPSLFLAHVPAFAFSVFTVGPAADCPYHSIQ
                     DAVNAAAGTAGVDYVWISNNLVSGARYNYVGQHIRVNDPDGVVIEGGFVSCADPNIDA
                     NENTTISGAGNDGGPVFDISSAGGDVFLGNLFITGANRAGDGGGIAFIGQVGGALILK
                     NTIITGNHAGYGAGINVNGANSPAALVLEDNNLIDFNTAATSGGGIRVEGNTRLYALS
                     PQTLIGYNHADGGYGGGIEVIGPARADIGSPGYNGLAAIYGNSALYGGGIAGVEDNNA
                     NPSQVRVFTVDAEHPVAISDNTASASGGALYVGGLQSYNNLCLYDFRIDDNTAPDGAA
                     IYVNTLGRVGINANPFESGADPCVSPEPPPQLGAVACALDAPCNEIDRNVAEDTSGNP
                     TEGSAISVQSDAWARGDRLRMQRNEGGHVIGAFATGFDTSSVDLNDCLLTDNVSTREL
                     IATGSDGISDLSMKSCTIAGNAVGAPFVFLSYGEFSLHNSIIDQPQRATVDEAGDSTF
                     IYADYVLTNDTATLLGHIGVAQGEPAFVDAGNGDYHLAPNSMGLDFAPSGTGSDLDRN
                     PRTVDLSAVVNLYGSMDLGAYELQSVPACSAADTIFCSGFEAYP"
     gene            complement(1994..2725)
                     /locus_tag="VFF05_05630"
     CDS             complement(1994..2725)
                     /locus_tag="VFF05_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HZW51317.1"
                     /translation="MGRQLALLYGVLCYLIFFATFLYAIAFVGGVFVPLSLDSDPTGP
                     FAESLLIDAILLGIFAVQHSVMARPAFKRWWTRFVPAAVERSTYVLFSSLALILLFWQ
                     WRPLGGMVWQVDSLVARDVLWALFALGWATVLIGTFLINHFDLFGLRQVWLNWRRTPY
                     TPPGFVTPLFYRVVRHPIMLGFIVAFWATPHMTLAHLIFAIATTAYILIALQLEEHDL
                     AASIGEPYRDYQRRVHMLVPLPRKS"
     gene            complement(2815..5616)
                     /locus_tag="VFF05_05635"
     CDS             complement(2815..5616)
                     /locus_tag="VFF05_05635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZW51318.1"
                     /translation="MTSPKDSTTQNALREAFDALLELPPEARVGWLETHVADAGLRER
                     LLDLLTAHDSRGFLDTTASEHVARLAARDLAPEGLIGSRIGAFRLVRALGQGGMSAVF
                     LGEREDADFRQQAAIKLLRRGLYSELEQRLFQRERRVLAALQHPNIARLIDGGVTAAG
                     IPYLIMEFVDGAPIMRYAEARNLDVRARVRLFVDVCRAVAEAHRNLIVHRDIKPSNIL
                     VAADGTVKLLDFGIAKLIEEGDTDATGTVGVFTPDYAAPEQLRGGAITTATDVYGLGV
                     LLHELLLGLKPGGAPTRRPSSRVDEAGDATVPRIERARLRKLLRGDLDNILLKALAEE
                     PQRRYASAGALADDIDNWLAGRAVAAHPPSRLYRTRKFVQRHRGGVVVTALFLAAVFA
                     ALGLALWQAQVARREAARANTVRDFVVGLFDAARAHLPRDQRPTPEALVEAARQRLAA
                     SDFDPLTQADLAQTLGEVELSMARFDRAQALFAEAAHAAASAADVRVLRHARVAHAQA
                     RQRAGDNAGALAELTPLLPELRAAPDADLLRALAVQAAAEMTLGKPDAAIADRREAAA
                     AASRLHGKSSQAALAAQFEVGNALAQAQRYPEAIAVLQPALATWRARHGAEDDRYVAA
                     YESLATANDGVGDLPGADARFRELLALEKRIYTTPHDAIATTLRDLGIILAREEKFSE
                     SESQLNQALAMQKQVFGGDHVELAKTWDALGTVAVDQRNFAEAQIDYGNAIAMCERAR
                     FADEVCPRAHNNLGMAYYRQDLLDDARAEMTKALAERRAMFGNDHPTVAYSLSTLANV
                     AVKQRDYKTAVDDSAQALAVLDRAGRGASREDALIRQGHAQALWLLDRNADALREIER
                     TLADWQRVAPQDKARRVTMLVQKAQILRDLKREDDARRTAIEAIGVGAAASELAPQTK
                     ALLRELSGKSDAYPEAAPR"
     gene            complement(5669..>5848)
                     /locus_tag="VFF05_05640"
     CDS             complement(5669..>5848)
                     /locus_tag="VFF05_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZW51319.1"
                     /translation="LDAALTQLEAADTRAARVVELRYFAGLPLEQVAEQLGVTRRTAD
                     RDWAFARAFLHDALA"
BASE COUNT         1006 a         1864 c         1998 g          980 t
ORIGIN      
        1 gttgacggtc agtgcgaaga gagcgaggaa agcaaagcgg atcgagcgtg acatgtgcag
       61 cctccctgag ggacacctgc gagtaacgcc gttgtgcggc aacatgcgac tcacgggtaa
      121 gcctcgaacc cgctgcagaa gatcgtgtcg gcggcgctgc acgcgggcac gctttgcagt
      181 tcatacgcgc ccaggtccat ggagccatac agattgacca ccgccgacaa atcgaccgtg
      241 cgcggattgc gatccagatc gctgcccgtg ccggaaggcg cgaagtcgag ccccatcgaa
      301 ttgggtgcca ggtgatagtc gccgttgccg gcatcgacga atgcgggttc tccctgcgcc
      361 acgccgatgt ggccaagcaa agtcgcggtg tcgttggtca agacgtaatc ggcgtagatg
      421 aatgtcgaat cgccggcctc atcgaccgtc gcccgctgcg gctggtcgat gatgctgttg
      481 tgcagcgaaa actccccata ggaaagaaaa acaaacggcg ccccgacggc gttgccggcg
      541 atggtgcagg atttcatgct caggtcactg attccatcgc tgccggttgc gatcaattcc
      601 ctggtcgaca cattgtcggt caacaagcaa tcgttcaaat cgacgctgct ggtgtcgaag
      661 ccggttgcga atgctccgat gacatggccg ccttcgttgc gctgcatgcg cagccggtcg
      721 ccgcgtgccc acgcgtcgct ctggacggag attgccgaac cctcggtggg attgccgctg
      781 gtgtcttcgg cgacgttgcg atcgatctcg ttgcaaggcg cgtccagcgc acatgccacg
      841 gcgcccagtt gcggcggcgg ctcgggcgac acgcacgggt cggcgccgct ttcgaacgga
      901 ttggcgttga tgccgactcg acccagggta ttgacataaa ttgccgcgcc atccggcgcg
      961 gtgttgtcgt cgatgcggaa gtcgtaaaga caaaggttgt tgtagctttg caagccgccg
     1021 acatacagcg ctccgccgct cgccgaggcg gtgttgtcgg aaatcgcaac cggatgttcc
     1081 gcgtcgaccg tgaaaacgcg aacctgggat gggttggcgt tgttgtcctc gacccctgcg
     1141 atgccgccgc cgtagagagc ggaattaccg tagatcgcgg ccaatccgtt gtaaccgggc
     1201 gagccaatgt ccgcgcgcgc ggggccgatc acttcgatgc ctccgccata accgccgtcg
     1261 gcgtggttgt agccgatcag ggtttgcggc gacagcgcgt acagtcgcgt gttgccttcc
     1321 acccgaatgc cgccgccgga agtggccgcc gtgttgaagt caatgaggtt gttgtcctcc
     1381 agcaccagcg ctgccggcga gttcgcgccg ttgacgttga tgccggcgcc gtaaccggca
     1441 tgatttccgg tgatgatggt gttcttcagg atcagcgcgc cgccgacctg gccgatgaac
     1501 gcgatgccgc caccgtcgcc ggcacgattc gcgccggtga tgaacagatt gccgaggaag
     1561 acgtcgccgc cggcactgga gatgtcgaac accggcccgc cgtcgttgcc ggcgccgctg
     1621 atcgtggtgt tttcgttcgc atcgatgttc ggatcggcgc aactgacgaa gccgccctcg
     1681 atgacgacgc catcgggatc gttcacgcgg atgtgctggc cgacgtagtt gtagcgtgcg
     1741 ccgctgacca ggttgttgct gatccacacg tagtccacgc cggcggtgcc ggcggcagca
     1801 ttcaccgcgt cttggatgga atggtagggg caatcggcgg ccgggccgac agtgaagacg
     1861 ctgaatgcga atgccggcac gtgcgcgagg aagagcgacg gcacgagcgg caatgccaac
     1921 cgccaaccac atggagtctt catcaacttt ctccgcaaaa tcccaaacat ccctgtgtcg
     1981 tcgaaatacc gcatcaggat tttctcggta gcggcacgag catgtgcaca cggcgctggt
     2041 aatcgcggta cggctcgcca atgctggcgg cgaggtcgtg ttcttcaagt tgcaatgcga
     2101 tcaggatgta cgccgtcgtc gcgatcgcga agatgagatg cgcgagcgtc atgtgcggcg
     2161 tggcccagaa cgcgacgatg aaaccgagca tgatcgggtg ccgcaccacg cgatagaaga
     2221 gcggcgtgac gaatccgggt ggcgtatagg gagtgcggcg ccaattcaac cagacctggc
     2281 gcaggccgaa caggtcgaaa tggttgatca ggaacgtacc gatcagcacc gtggcccagc
     2341 cgagcgcgaa tagcgcccag agaacgtcgc gggcaaccag gctatcgacc tgccagacca
     2401 tcccgccgag tggacgccat tgccagaaca gcaagatcaa ggcgaggctg gaaaacagca
     2461 cgtaggtgct gcgctcgacg gcggcgggca cgaagcgcgt ccaccagcgt ttgaaggcgg
     2521 gccgtgccat gacgctgtgc tgcacggcga agataccgag cagaatcgcg tcgatcagca
     2581 gggattcggc gaagggtccg gtggggtcag aatccagcga cagcggcacg aagacgccgc
     2641 cgacgaaagc gatggcgtac aaaaacgtgg cgaagaaaat cagatagcag agtaccccgt
     2701 acaacaaagc aagctgacgg cccatgacgc acctcctcga atgccggccg gcgccggatg
     2761 gcgcggctgc agcgaggaac gccggacggc atgaaattgc gacatgccgc tttctcagcg
     2821 cggcgccgct tccggatacg cgtcggattt gccggacagc tcgcgcagca gtgccttggt
     2881 ctgcggggcc agttcgctgg ccgctgcacc gacaccgatg gcttcgatcg cggtgcggcg
     2941 cgcgtcgtct tcacgcttga ggtcgcgcag gatctgcgcc ttctgcacca acatcgtcac
     3001 acgccgcgcc ttgtcttgcg gcgccacgcg ctgccagtcg gcgagcgtgc gctcgatctc
     3061 gcgcagggcg tcggcgttgc ggtcaagcag ccacagtgct tgcgcgtgac cttggcggat
     3121 cagcgcgtcc tcgcgcgagg cgccgcgacc ggcgcggtcg agtacggcca gcgcctgcgc
     3181 cgaatcgtcc acggcggtct tgtaatcgcg ctgcttgacc gcgacgttgg ccagcgtcga
     3241 cagcgaatac gccacggtcg gatggtcgtt gccgaacatc gcgcggcgtt cggcgagcgc
     3301 cttggtcatt tccgcgcgcg catcgtcgag caggtcctgc cggtagtagg ccatgcccag
     3361 gttgttgtgc gcgcgcggac acacctcgtc ggcgaagcgc gcgcgctcgc acatggcgat
     3421 tgcgttgcca taatcgattt gcgcctcggc aaaattgcgc tggtcgacgg cgacagtgcc
     3481 gagtgcatcc caggtcttgg ccagttccac gtgatcgccg ccgaacactt gtttctgcat
     3541 agccagtgcc tgattgagct gcgattcgga ttcgctgaac ttttcctcgc gcgcgaggat
     3601 gatgccgaga tcgcgcaagg tcgtggcgat ggcgtcgtgc ggagtggtgt agatgcgttt
     3661 ctcgagcgcc agcaattcgc gaaaacgcgc gtcggcgccg ggcaggtcgc cgacgccgtc
     3721 gttcgccgtg gccagactct cgtaagccgc gacatagcgg tcgtcctccg cgccgtgccg
     3781 cgcacgccag gtcgcgagcg ccggctgtag aaccgcgatc gcttcgggat agcgctgcgc
     3841 ctgcgccagc gcgttgccga cttcgaattg cgctgccagc gccgcttggc tgctcttgcc
     3901 gtgcagtcgc gatgccgcgg cagcggcttc gcgccggtcc gcgatcgcgg cgtcgggctt
     3961 gccgagcgtc atttccgccg cggcctgcac ggccagcgcg cgcagcaggt cggcatccgg
     4021 cgcggcgcgc agttcgggca gcagtggcgt caattcggcg agcgcgccgg cattgtcgcc
     4081 ggcacgctgc cgcgcttgcg catgcgcgac gcgtgcgtga cgcaggacgc gcacgtcggc
     4141 ggcgctggcg gcggcgtggg cggcttccgc gaacagggct tgtgcgcgat cgaaacgcgc
     4201 catcgacaat tctacttcgc cgagcgtctg cgcgagatcc gcctgcgtga gcggatcgaa
     4261 atcgctcgcc gccaagcgtt gccgcgctgc ttcgacgagc gcctcgggcg tcgggcgctg
     4321 gtcgcgcggc agatgcgcgc gcgcggcgtc gaacaggccg accacgaagt cgcgcaccgt
     4381 gttggcgcgc gccgcttcgc gccgcgccac ctgcgcctgc cacagggcaa ggccgagcgc
     4441 ggcgaagacc gcggccagaa acagggcggt caccaccacg ccgccgcggt ggcgctgcac
     4501 gaacttgcgc gtgcggtaca ggcgcgacgg tggatgcgcg gcgaccgcgc gcccggcgag
     4561 ccagttgtcg atgtcgtctg cgagcgcgcc ggccgaggcg taacggcgct gtggttcctc
     4621 ggccagcgcc ttgagtagga tgttgtcgag gtcaccacgc agcaacttgc gcaggcgcgc
     4681 acgctcgatg cgtggaaccg tcgcatcgcc cgcttcgtcg acgcgcgagg atggccgccg
     4741 cgtcggcgcg ccgccgggct tcaggcccag cagcagttcg tgcagcagca cgcccaggcc
     4801 atacacatcg gtcgcggtgg tgatcgcgcc gccgcgcaat tgttcgggtg cggcgtagtc
     4861 gggcgtgaac acgccgaccg tgccggtcgc gtcggtatcg ccttcctcga tcagcttggc
     4921 gatgccgaaa tcgagcagct tcaccgtgcc gtcggccgcg acgagaatgt tcgatggttt
     4981 gatgtcgcga tgcacgatca ggttgcggtg cgcctcggcg acggcgcgac acacgtcaac
     5041 gaacaggcgc acgcgcgcac gcacgtcgag attgcgcgct tcggcgtaac gcatgatcgg
     5101 cgcaccgtcg acgaattcca tgatcaggta gggaatgccg gccgcggtga cgccgccgtc
     5161 gatcaggcgc gcgatgttgg gatgctgcaa tgccgccagc acgcgccgct cgcgctggaa
     5221 cagacgctgt tccagttccg aatacaggcc acggcgcagc agcttgatcg cggcctgctg
     5281 gcggaaatcc gcatcctcgc gctcgccgag gaagaccgcc gacataccgc cctggccgag
     5341 cgcgcgcacg aggcgaaacg cgccgatgcg gctgccgatc aggccttcgg gcgcgagatc
     5401 gcgcgccgcc aggcgcgcga cgtgctcgct ggcggtggta tccagaaagc cgcggctgtc
     5461 gtgcgcggtg agcaggtcga gcaggcgctc gcgcaggccc gcatcggcca cgtgcgtttc
     5521 cagccagccc acgcgcgctt ccggcggcag ctcgagcagc gcatcgaagg cctcgcgcaa
     5581 cgcattttgc gtggtggaat ctttgggcga cgtcattgct gcgaagaacg ccatgacgcg
     5641 gcaggatgcg acatgcggga acccacggtc aggccagcgc gtcgtgcagg aaagcgcgcg
     5701 cgaatgccca atcgcggtcc gcggtgcgcc gggtcacgcc gagttgttcg gcgacctgtt
     5761 ccagcggcag gccggcgaaa tagcgcaact cgaccacgcg cgcggcgcgc gtgtcggcgg
     5821 cttccagttg cgttagcgcg gcatccag
//
LOCUS       DASRVO010000454         1028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_40074, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000454 DASRVO010000000
VERSION     DASRVO010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40074"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1028
                     /locus_tag="VFF05_05645"
     CDS             <1..>1028
                     /locus_tag="VFF05_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51320.1"
                     /translation="SLVTHYALAHPECQFSLKTPTQNILDCAPVEKLADRVYQIFGRQ
                     ALDELVEIPAQESPVRSAITEPQIEADEQAAMLRISGFASRPEVQRGNRNGIYIFVNR
                     RLVRDRLLLHAIGEAYRNIMPPGAFPALLLFLDLPYQEVDVNVHPAKIEVRFRHPHFV
                     HDFTRDSLRQALSIARPIPTFPAARAAAAAIPADAAAAPAVVDGAGSMRGYPPMPDAA
                     AFPGPPRAEIPLAPSYGAYGASGAATDPFELSGAPDRPVPQRLQFGSDSGMNFPFGAS
                     AASAASSDAGAATAAAPISESATLPSADQIMDLKPLGQVNSSFIVAVNREGLWIVDQH
                     VAHERVLF"
BASE COUNT          172 a          361 c          309 g          186 t
ORIGIN      
        1 tcgctggtca cgcactacgc gctggcgcat cccgagtgcc agttttccct gaaaacgccg
       61 acgcagaaca ttctcgattg cgcgcccgtc gaaaaactgg cggatcgcgt gtaccagatt
      121 ttcggccgcc aggcgctcga cgagctggtc gagattcccg cgcaggaatc gcccgtgcgc
      181 tccgccatta ccgagccgca gatcgaggcg gacgagcaag ccgcaatgct gcgcatcagc
      241 ggtttcgcct cgcgcccgga ggtgcagcgc gggaaccgca acggcatcta cattttcgtg
      301 aatcgccgcc tggtgcgcga tcgcctgctg cttcacgcca tcggcgaagc ctatcgcaac
      361 atcatgccgc ccggcgcgtt tcccgcgctg ctgctattcc tcgatttgcc gtaccaggaa
      421 gtcgatgtca acgtgcaccc ggcgaaaatc gaggtgcgct tccggcatcc gcatttcgtg
      481 catgacttca cgcgcgattc gctgcgccag gcgctctcca tcgcgcggcc gattccaacg
      541 tttcccgcgg cgcgcgcggc tgcagctgcc ataccagcag atgctgccgc cgcacctgca
      601 gtcgtcgacg gagcaggaag catgcgcggc tacccgccca tgcccgacgc tgctgcgttt
      661 cccggacctc cgcgcgcgga aattcccctg gcgcccagtt atggcgcata cggcgcgagc
      721 ggcgctgcga cagatccatt cgagctgagc ggcgcgccgg atcggcccgt cccgcagcgc
      781 ctgcaattcg gatcggacag cggcatgaat tttccgtttg gcgcttcagc cgcttctgca
      841 gcttcttcgg atgccggcgc agcgacggct gcagcgccca tttccgagtc cgccacgctt
      901 cccagcgccg atcaaatcat ggacctgaag ccgctcgggc aggtgaacag cagcttcatc
      961 gtcgccgtga accgcgaagg attgtggatc gtcgaccagc acgtggcgca cgagcgcgtg
     1021 ctcttcga
//
LOCUS       DASRVO010000455         5375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_325326, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000455 DASRVO010000000
VERSION     DASRVO010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325326"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFF05_05650"
     CDS             complement(<1..346)
                     /locus_tag="VFF05_05650"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014503633.1"
                     /note="catalyzes the conversion of N-acetylornithine to
                     N-acetylcitrulline in an alternative arginine biosynthesis
                     pathway; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HZW51321.1"
                     /translation="MTIRHFLSTQDWSRVELDALLKQAAAFKHSKVGTQLAGKSIALL
                     FFNPSMRTRTSFELGAFQLGGHAVVLQPGKDAWPIEFDVGTVMDGEAEEHVAEVARVL
                     SRYVDLIGVRAFP"
     gene            complement(597..1163)
                     /locus_tag="VFF05_05655"
     CDS             complement(597..1163)
                     /locus_tag="VFF05_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HZW51322.1"
                     /translation="MSLLRIFPFVFLTLLSAQTFAQAPPPAPVATGEENSHFGWADVL
                     RVDPIYGDDATSPPAQPCYEEQVPVDAAADTEVQNKRGVFTAIGAFVGGILGNQFGRG
                     KGRAAATVAGAIAGGVAGNNIAAADEGDDGRPQYKTVRHCPPAASSQRKVVAYDVEYR
                     YRGDVYSSRMSYDPGDRLRVKVSVSPAE"
     gene            1411..2823
                     /gene="cysS"
                     /locus_tag="VFF05_05660"
     CDS             1411..2823
                     /gene="cysS"
                     /locus_tag="VFF05_05660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZW51323.1"
                     /translation="MQLRLHNSRTRRVEDFAPADPLRTTIYLCGPTVYNYVHIGNARG
                     PVVFGVLVRLLRHVYGAAHVVYARNVTDVDDKINAAAAESGVAIDVITRRYAAAYDED
                     MARLGVDAPDIVPYATAHIPQIITMCETLIAHGHAYAAQGHVLFDVSSYADYGQLSGR
                     STDEMIAGARIDVAPYKKNPADFVLWKPSTPDLPGWDSPWGRGRPGWHIECSAMSAAH
                     LGETIDIHAGGNDLLFPHHENEVAQSTCAHAGKLFARFWLHNGMLTFDGKKMSKSLGN
                     TLQLHELLQQHPPELLRYMLVKAHYRQPLDWSDTTLEQARRTLDGLYGTLRDLADVAA
                     DPGPVPDALLAALADDLNTPEALAVLARLADEARKSTNPQDKKAAKAALLGAGKFLGL
                     LQVDPEAWFKGAADSVDVAWVENLLAERAEARKRRDFAASDRIRDTLAAKGIVIEDSP
                     QGARWKRIIHAGENQDECAA"
     gene            2807..3247
                     /locus_tag="VFF05_05665"
     CDS             2807..3247
                     /locus_tag="VFF05_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HZW51324.1"
                     /translation="MNAQPDIARIESAQSEQDAIIEEFAFFGDWTERYQYLIDLGRKL
                     PPFPEELKTEQYKVSGCQSQVWLVADGDARALTFRAISDSAIVSGLIALLLRVYSGRS
                     AAEILDTPPRFIEAIGLAQHLSPTRSNGLAAMLKRIKDEARARV"
     gene            complement(3389..3868)
                     /gene="dksA"
                     /locus_tag="VFF05_05670"
     CDS             complement(3389..3868)
                     /gene="dksA"
                     /locus_tag="VFF05_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HZW51325.1"
                     /translation="MSVVAEKDTVTREDGRYALPSTTIIELPANYRPSPREEYMGPKQ
                     LAYFRAKLQKWREDLVEESKQTIDNLRDEVRDVGDEAERATRETENSLELRTRDRYRK
                     LIAKIDKALKRIEEGRYGFCEETDEDIGLERLDARPIATLCLDAQERWEHRQKQMGD"
     gene            3906..4154
                     /locus_tag="VFF05_05675"
     CDS             3906..4154
                     /locus_tag="VFF05_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51326.1"
                     /translation="MAFLVDLTGLGAGFFAATFLFGLIGLDALRDTAFGAGLCAGFFA
                     AAFFTGFADFFATGFFAAGLRLEFAMLLTTLSLSGRVL"
     gene            4185..4436
                     /gene="yidD"
                     /locus_tag="VFF05_05680"
     CDS             4185..4436
                     /gene="yidD"
                     /locus_tag="VFF05_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZW51327.1"
                     /translation="MTRILLFLLAAYKRLLSPLLGARCRFHPSCSDYARVAVARFGAL
                     RGGVLALWRIVRCQPLCRGGFDPVPERFTMRRLPDKDSQ"
     gene            4433..>5375
                     /locus_tag="VFF05_05685"
     CDS             4433..>5375
                     /locus_tag="VFF05_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZW51328.1"
                     /translation="MSDWLIVNADIVNEGRRFAADLRVRDGRIKSIAPKLSAQPGERV
                     FDARGRLLMPGMIDDHVHFREPGYTAKADIASESRAAVAGGVTGFIEMPNTNPPTLTH
                     ALLEEKYALAARGSVANYAFYFGASNDNLDAIRALDPRTVPGVKVFLCESTGNLCVSD
                     PATLDGVFRDSPVLVAVHCEDDRIVHANIDRARAQYGDAIPMRMHAEIRSRQACFDST
                     KWAVETARRHGTRLHVLHVSTADELALFEAGPVAGKRITAETCVHFLRYTDADYERLG
                     GRIKCNPSIKAASDRDALMRALVDTRLDILATDHAPHL"
BASE COUNT          981 a         1762 c         1663 g          969 t
ORIGIN      
        1 tcgggaacgc gcgcacgccg atgagatcca cgtagcgcga cagcacgcgc gcgacctcgg
       61 cgacatgttc ctcggcctcg ccgtccatga ccgtgccgac gtcgaattcg attggccagg
      121 cgtccttgcc gggctgcagc acgacggcgt ggccgccgag ctggaacgca cccagttcaa
      181 aactcgtgcg cgtgcgcatc gacggattga agaacaacag cgcgatcgat ttgccggcaa
      241 gctgcgtgcc gaccttcgaa tgcttgaatg cggcggcctg cttgagcagc gcgtcgagct
      301 cgacccgcga ccagtcctgg gtggaaagaa aatggcgaat cgtcacgata agaactccag
      361 aaaacgaaaa acccggcgcg aggccgggtt ttcagatcga tgcgacaaag aaacgtgcgc
      421 gcgacagcta cccggccgaa atgcaaggct ccggtcggcg cgcgcgcgaa gtcatgccgg
      481 cggccatgcg ggcgctggtc aggacattgg tggtggaaat cggaaagttc atggcagcca
      541 cgttatcacg aacgagtttt gcggcgcaac atgtttcgaa aaggacgtct ggacggctat
      601 tccgccggag acacgctcac cttcacgcgc aggcggtcgc cagggtcgta ggacatgcgc
      661 gagctgtaga catcgccgcg atagcggtat tcgacgtcgt aagcgacgac cttgcgctga
      721 ctcgatgcgg cgggtgggca atgccgcacc gtcttgtact gcggccggcc atcgtcgccc
      781 tcatccgcgg cggcgatgtt gttgccggcc acgccaccgg caatggcgcc ggccacggtc
      841 gccgccgcgc ggcccttgcc cctgccgaac tggttgccga ggatgccgcc gacgaaggcg
      901 ccgatcgcgg tgaagacgcc gcgcttgttc tgcacttctg tatccgcagc cgcgtcgacc
      961 gggacctgtt cctcgtagca aggttgcgcc ggcggactcg tcgcatcgtc accatagatc
     1021 ggatccacgc gcagcacatc agcccagcca aaatgcgaat tctcctcgcc cgtcgctaca
     1081 ggcgccggcg gcggcgcctg cgcgaaagtc tgcgccgaaa gcagcgtcaa gaagacgaat
     1141 ggaaaaatgc gcaacaggct catggcagtt cgcgacacgc tggcaacacg ttggttacac
     1201 gttggtaatt ggggttccgg gcatcctgcc gcaacgtctc tgggatccgc agcattccga
     1261 tactcgggct gccgacggca cgaatttaag cacccgcagg ctgaatgaca cttaaggcaa
     1321 accccgtcgt ggcgcggccc gcaggccatg cgatcctgaa cgaaacgcat gcctgcgcta
     1381 caatgaagca tcacaataac cggcctgccg atgcaactgc gcctgcacaa ttcccggact
     1441 cgccgcgtcg aggatttcgc acccgcggat ccactccgca cgaccattta tctgtgcgga
     1501 ccgaccgtct acaactacgt gcatatcggc aacgcacgcg gaccggtcgt gttcggcgtg
     1561 ctggtgcgcc tgttgcggca tgtttacggc gcggcgcacg tcgtgtacgc gcgcaacgtc
     1621 accgacgtcg acgacaagat caatgcggca gcggccgaat ccggcgtggc catcgacgtc
     1681 atcacgcggc gctacgcggc tgcctacgac gaggacatgg ccaggctcgg cgtcgatgcg
     1741 ccggacatcg taccttacgc gaccgcgcac attccgcaaa tcatcacgat gtgcgaaacc
     1801 ctgatcgcac acgggcatgc ctatgccgcg cagggccacg tgttgttcga tgtttcgtcg
     1861 tatgcggact atggccagct ctcgggccgc tccaccgacg agatgattgc cggcgcgcgc
     1921 atcgacgtgg cgccgtacaa gaagaatccc gccgacttcg tgctgtggaa gccgtccacg
     1981 ccggatttgc cgggctggga cagtccctgg ggccgtggcc gacccggctg gcacatcgaa
     2041 tgctcggcga tgagcgcggc gcacctgggc gagaccatcg acatccacgc cggcggcaac
     2101 gatctgctgt ttccgcatca cgaaaacgaa gtcgcacaat cgacttgcgc gcacgccggg
     2161 aaactcttcg cgcgtttctg gctgcacaac ggcatgctca ccttcgatgg aaaaaaaatg
     2221 tcgaagtcgc tgggcaatac cctgcaactg cacgaactgc tgcaacaaca tccgccggaa
     2281 ctgctgcgct acatgctggt gaaggcgcat taccggcagc cgctggactg gtcggatacg
     2341 acgctggaac aggcgcggcg cacactcgac ggactttacg gaaccctgcg cgacctggcc
     2401 gatgtggcgg ccgatccggg cccggtgccg gacgcgctgc tcgccgcgct cgccgacgac
     2461 ctcaacacgc ccgaggcgct ggcggtgctc gcgcgactcg cggacgaggc acgcaaatcc
     2521 acaaatccac aagacaagaa agcggcaaaa gcggcgttgc tcggcgccgg caagttcctc
     2581 ggcctgctgc aagtcgatcc ggaagcctgg ttcaagggcg ccgcggacag cgtcgatgtc
     2641 gcgtgggtcg aaaacctgct cgcagaacgc gccgaggcgc gcaagcgccg cgatttcgcc
     2701 gccagcgacc gcatccgcga cacgctcgcc gcgaaaggca tcgtgatcga ggacagcccg
     2761 cagggcgcac gctggaaaag gatcattcat gccggagaaa atcaggatga atgcgcagcc
     2821 tgatatcgcc cgcatagagt cggcgcagtc cgaacaggac gccatcatcg aggagttcgc
     2881 cttcttcggc gactggaccg aacgctacca gtacctgatc gacctcggcc gcaagctgcc
     2941 gccgttcccg gaagaattga agaccgagca atacaaggtt tccggctgcc aatcgcaggt
     3001 ctggctggtc gccgacggcg acgcgcgcgc gctcacattc cgcgcgatca gcgattcggc
     3061 catcgtctcc ggcctgatcg ccctgctgct gcgcgtctac tcgggacgtt cggcggcgga
     3121 aatcctcgac acgccgccgc gcttcatcga agccatcggc ctcgcccagc atctttcgcc
     3181 gacccgcagc aacgggctgg cagcgatgtt gaaacggata aaagacgagg cgagagcgag
     3241 agtctagagc cgcagtcaaa atcgtggcga gcgaaggcag ttcgcgggac gccggagcgg
     3301 aggaaccgga gttgacacgt cagtcaatga ggattccgag caccgccgaa ccgcgaaatg
     3361 ccgagcgcag cagattttgg cgcggctctc agtcgcccat ctgcttctgt ctatgctccc
     3421 agcgctcctg cgcatccaga cacagcgtcg cgatcggacg cgcatcgagg cgttccaggc
     3481 cgatgtcctc atcggtttcc tcgcagaaac cgtagcggcc ttcctcgatg cgcttgagcg
     3541 ccttgtcgat cttggcgatc agcttgcggt aacggtcgcg ggtgcgcaat tccagagagt
     3601 tctcggtctc gcgcgtggcg cgttcggcct cgtcgccgac atcgcgcact tcgtcgcgca
     3661 ggttgtcgat ggtctgcttg gattcctcga ccagatcctc gcgccacttc tgcaatttgg
     3721 cgcggaagta cgcgagctgc ttcgggccca tgtattcctc gcgcggactc ggccggtaat
     3781 tggccggaag ctcgatgatc gtggtgctgg gcagggcata acggccgtct tcgcgggtga
     3841 cggtatcttt ttcggcaacg actgacattt ttcctttctt cgcagcggcc gctggtttgt
     3901 gcggcgtggc tttcctggtt gatttgaccg gcttgggcgc gggttttttc gccgcaacct
     3961 tcttgttcgg cttgatcggc ttggacgctt tgcgcgatac agccttcggc gctggtttat
     4021 gcgccggttt tttcgccgcc gccttcttca ccggtttcgc ggatttcttc gccacaggct
     4081 tcttcgcagc cggtttgcgt ttggaatttg ccatgttact caccacattg tccctcagcg
     4141 gtcgcgtgtt atagcctaag cgaccccatg gggcaaccct tttcgtgaca cgcatactcc
     4201 tcttcctgct cgccgcctac aagcgcctgc tcagtccgct gctcggcgcg cgctgccgct
     4261 tccacccgag ctgctcggat tacgcccgcg tcgcggtggc gcgcttcggc gcgctgcgcg
     4321 gcggcgtgct tgcgctgtgg cgcatcgtgc gctgccagcc gctgtgccgc ggagggttcg
     4381 atccggtgcc ggaaaggttt acgatgcggc gtttgcccga caaggattcg caatgagcga
     4441 ttggctgatc gtcaacgccg acatcgtcaa cgaaggccgg cgtttcgccg cagatttgcg
     4501 tgtgcgcgac ggccggatca agtctatcgc accaaagctt tcggcccaac ccggtgaacg
     4561 cgtgttcgac gcgcgtgggc gcctgctcat gcccggcatg atcgacgatc acgtgcattt
     4621 ccgcgaaccc ggctacacgg ccaaggccga cattgcctcc gaatcgcgcg ccgccgtcgc
     4681 cggcggcgtg accgggttca tcgagatgcc caacacgaat ccgccgacgc tgacccacgc
     4741 attgctggaa gaaaaatacg cgctcgccgc gcgcggttcc gtggccaatt acgccttcta
     4801 tttcggcgcc agcaacgaca acctggatgc gatccgtgcg ctcgatccgc gcacggtgcc
     4861 gggggtgaag gtgttcctgt gcgaatcgac cggcaacctg tgcgtcagcg atccggcgac
     4921 gctggacggc gtgtttcgcg attcgcccgt tctcgtcgcc gtgcattgcg aggacgaccg
     4981 catcgtgcac gccaatatcg accgcgccag ggcgcaatac ggcgacgcga tcccgatgcg
     5041 catgcacgcg gaaatccgct cgcgccaggc ttgtttcgac tcgacgaaat gggcggtcga
     5101 aaccgcgcgt cgccacggca cgcgcctgca cgtgttgcac gtctccacgg cggacgaatt
     5161 ggcgctgttc gaggccggcc ctgtcgccgg caagcgcatc accgccgaaa cctgcgtgca
     5221 tttcctgcgc tacaccgacg cagattacga acgtctcggc ggccgcatca agtgcaatcc
     5281 ctcgatcaag gctgcttccg accgcgatgc gttgatgcgc gcgctcgtcg atacgcgact
     5341 ggacatcctc gccaccgacc acgcgccgca cctgg
//
LOCUS       DASRVO010000456         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_273551, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000456 DASRVO010000000
VERSION     DASRVO010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273551"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="VFF05_05690"
     CDS             complement(<1..1681)
                     /locus_tag="VFF05_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HZW51329.1"
                     /translation="MSEDRKPKVEPYTAPAGGWGSVKSVTQILRREKVPIPSTIKELW
                     RQNKPRGFTCVSCAWPQPEKPLAFEFCENGAKASAWELTSLRTTPEFFAQHTCAELQQ
                     WSDHDLEQQGRLTQPMRYDRASDKYVPCAWHEAFAAIGAELKKLDPRSVVFYASGRAS
                     LETSYLYALFARMYGHNNLPDSSNMCHEATGVALRQCIGTPVGTVVLDDFAKTDCIFF
                     FGQNAGVNSPRMLHDLQNAAKRGVPIIVFNPVRERGLERFANPQSPLEMLTGRQTKLA
                     TQYHQVKTGGDIAAITGLCKTLLALDDEAAKNGAPRVLDTTFIAQHCHGFETFADFVR
                     AQSWEAIEADSGLTRAALEGAAAVYARANAVIGVYGMGLTQHVLGEYNIYMVASLLLM
                     RGNLGKPGAGICPVRGHSNVQGQRTVGIADDPALVPLDRLAEQYGFSPPRERGLHTVN
                     ACKGVLDGTVHGFIGLGGNFLRAIPDQSRVIAAWDKLALTVQIATKLNRTHLVNGKVA
                     YLLPCLGRIERVMENGKAQVLSTEDSTSCIHASVGKAEPASSSLLSETRIVI"
     gene            1778..>2090
                     /locus_tag="VFF05_05695"
     CDS             1778..>2090
                     /locus_tag="VFF05_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZW51330.1"
                     /translation="MSADPTYTLAQESAAVANRGRARRAVVVCQEGEAQHGDDWLVEE
                     APVELRYNGDAFAVMMATPADLEDFACGFALSENHVSGIDAIRGVTVAELLDGYVIDI
                     HA"
BASE COUNT          363 a          661 c          668 g          398 t
ORIGIN      
        1 cgatcacgat gcgcgtttcc gacaacagac tggaactggc gggttccgcc ttgccgaccg
       61 aagcgtggat gcaactggtg gaatcctcgg tggacagcac ctgagccttg ccgttttcca
      121 ttacccgctc gatgcggcca agacacggca acaaatacgc gaccttgcca ttgaccagat
      181 gcgtgcggtt caatttggtc gcgatctgca cggtgagggc cagtttgtcc caggccgcga
      241 tcacgcgcga ctgatccgga atcgcgcgca ggaagttgcc gccgagtcca atgaatccgt
      301 gcacggtgcc atccagcacg cccttgcaag cattcacggt gtgcaagccg cgttcgcgtg
      361 gcggcgaaaa tccgtattgt tcggccaggc gatccagtgg caccagggcg ggatcgtcgg
      421 cgatgccgac cgtgcgctgg ccctgcacgt tggaatgtcc gcgcaccggg cagatgcccg
      481 cacccggttt gccgaggttg ccgcgcatca gcaacaggct cgcgaccatg tagatgttgt
      541 attcgcccaa tacatgctgg gtgaggccca tgccgtacac gccgatcacc gcgttcgcac
      601 gggcgtagac cgcagccgcg ccttcaagtg ccgcgcgggt caggcccgag tccgcctcga
      661 tcgcttccca cgattgcgcg cgcacgaaat ccgcgaacgt ctcgaaaccg tgacagtgtt
      721 gcgcgatgaa ggtcgtgtcg agcacgcgcg gtgcgccgtt cttcgccgct tcgtcgtcca
      781 gcgcgagcag ggtcttgcac aggccagtga tcgcggcgat gtcgccgccg gtcttgacct
      841 ggtgatattg cgtcgcgagc ttcgtctgcc tgcccgtgag catttccagc ggactttgcg
      901 gattggcgaa acgctccagc ccgcgttcac gcaccggatt gaacacgatg atcggcacgc
      961 cgcgtttcgc cgcattttgc aaatcgtgca acatgcgcgg gctgttcacg cccgcgttct
     1021 ggccgaagaa gaagatgcag tcggtcttgg cgaaatcgtc cagcaccacc gtgccgaccg
     1081 gcgtgccgat gcactggcgc agcgccacgc cggtggcctc gtggcacatg ttggatgagt
     1141 ccggcaggtt gttgtggccg tacatgcgcg cgaacagtgc gtacagataa cttgtctcca
     1201 gcgaagcgcg accggaagcg tagaacacca ccgatctcgg atcgagcttc ttcagttccg
     1261 caccgatcgc cgcgaacgct tcgtgccatg cgcagggcac gtacttgtcg ctggcgcggt
     1321 cgtagcgcat cggctgcgtc agacggccct gctgctcgag atcatggtcg gaccactgct
     1381 gcaattccgc gcaggtgtgc tgcgcgaaga actccggcgt agtgcgcagg ctggtcaatt
     1441 cccaggcgct ggccttggcg ccgttctcgc agaactcgaa cgccagcggt ttttccggtt
     1501 gcggccacgc acaggacacg caggtgaatc cgcgcggctt gttctgccgc cacaattcct
     1561 tgatggtgga tggaatcggc accttctcgc gccgcaggat ttgcgtcacc gatttcaccg
     1621 aaccccagcc gccggcgggg gcggtgtagg gctcgacctt gggtttgcga tcctcgctca
     1681 cggccagcac ctcgcgatac cgtcggatcc gattatgcag caatgccggc atgcgggtgc
     1741 gcaatcggcg cgcgcagggc tatgatcggg agcatccatg tcagccgacc ctacctacac
     1801 gcttgcgcaa gagtccgccg ccgtggcgaa ccgcggccgg gcgcggcgtg ccgtcgtcgt
     1861 ttgccaggaa ggcgaagcgc agcacggcga cgactggctg gtggaggaag cgccggtcga
     1921 actgcgctac aacggcgatg catttgcggt gatgatggcc acgccggcgg atctggaaga
     1981 tttcgcctgc ggattcgcgc tgagcgaaaa ccatgtttcc ggcatcgatg ccatccgtgg
     2041 cgtgaccgtc gctgaattgc tggatggcta tgtgatcgac atccatgccg
//
LOCUS       DASRVO010000457         1617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_168203, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000457 DASRVO010000000
VERSION     DASRVO010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168203"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /gene="hemA"
                     /locus_tag="VFF05_05700"
     CDS             complement(<1..601)
                     /gene="hemA"
                     /locus_tag="VFF05_05700"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZW51331.1"
                     /translation="MTLIALGLNHQTAPLPVREKVAFAPEAMPAALSDLARQAGVHEA
                     LILSTCNRTELYVDVATGAETVPRDWLLGHHDLDVRRLDEFLYRHADYAAVRHLFRVA
                     TGLDSMVLGEPQILGQVKDAYQLARDAGTLRAPMERLLQQTFAVAKRVRTETRIGANP
                     VSVAFTAVRLAERLFADLSQACVLLIGAGETMELAARHLA"
     gene            654..>1617
                     /locus_tag="VFF05_05705"
     CDS             654..>1617
                     /locus_tag="VFF05_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51332.1"
                     /translation="MTTLYRLHAAIFAAGFAAIVAGCASVPAGRQAAGSKQPLQHLAV
                     APVTPEEDVLAMKLAGQFALESGDVARAAHEFARAAALGHDADLADEAVEVAIAAKDW
                     DLARSGITRWQQLRADDPALWQARASIALHDGQPDAALEDLRKLAHEPDGKGWSAIGR
                     VLVAHGGKAQAGALLQKLATPEALGKKSATWVAVSELADRLGADVLAGTLAKDAIARF
                     GEASAYVWGAELKLKSGDKSGAHALFVEALKRNAGDARLRVAYATVLGQMGEYAEAAK
                     VLGTGKQDDYSYAARAAYAARANDKTLIAPLYQELLADKTPRNGA"
BASE COUNT          290 a          522 c          559 g          246 t
ORIGIN      
        1 cggccagatg gcgggcggca agttccatgg tttcgcccgc gccgatcagc agcacacagg
       61 cttggctcag gtccgcgaaa aggcgttcgg ccaggcgcac ggcggtgaat gcgaccgata
      121 ccggattggc gccgatgcgc gtctccgtac gcacgcgctt ggccacggcg aaagtctgct
      181 gcaacaggcg ttccatcggc gcgcgcaggg tgccggcatc gcgcgcgagc tggtaggcgt
      241 ccttcacctg gccgaggatc tgcggttcgc cgagcaccat cgaatccaga ccggtcgcga
      301 cgcgaaacag gtgccgcacc gcggcatagt cggcgtggcg gtagaggaac tcgtcgagcc
      361 ggcgcacgtc caaatcgtga tggccgagca gccagtcgcg cggcacggtt tcggcgccgg
      421 ttgccacgtc cacgtacaac tccgtgcggt tgcaggtgga caggatcagg gcttcgtgca
      481 cgccggcctg acgcgccaga tccgacaatg ccgccggcat cgcctcgggc gcaaacgcca
      541 ccttttcgcg caccggcagg ggcgcagtct ggtggttgag gccaagagcg atcagggtca
      601 tgcaagcacg gtcatgtcgg ctaaactaca agcgtatcga ctggaaaatt tcgatgacaa
      661 cattgtaccg ccttcacgcc gccatctttg cggccggctt tgccgcgatc gtggccggat
      721 gcgccagtgt gccggccggg aggcaggccg ccggttcgaa gcagccgctg cagcatctgg
      781 cggttgcgcc ggtgaccccg gaggaagatg tgctggcgat gaaactggcc ggccagttcg
      841 cgctggaaag cggcgatgtc gccagggcgg cgcatgaatt cgcgcgcgcg gcggcacttg
      901 gccacgatgc cgatcttgcc gacgaggcgg tggaagtcgc catcgccgcc aaggactggg
      961 atctggcgcg ttccggcatc acgcgctggc agcaattgcg cgcggacgat cccgcgttgt
     1021 ggcaggcgcg cgccagcatc gccttgcacg acggtcagcc cgatgcggcg ttggaagacc
     1081 tgcgcaagct tgcgcacgaa cccgatggca agggctggag cgcgattggc cgcgtcctcg
     1141 tcgcgcacgg cggcaaggca caagccggcg cgctgctgca aaagctggcc acgcccgaag
     1201 cgcttggcaa gaaatccgcc acctgggttg cggtgagcga attggccgat cgcctgggcg
     1261 ccgatgtgct ggccggtaca ttggccaagg atgccatcgc gcgtttcggc gaggcatcgg
     1321 cctacgtctg gggcgccgaa ctcaagctca aatccggcga caaatccggt gcgcacgcct
     1381 tgttcgtcga agcgctcaag cgcaacgccg gcgatgcgcg tctgcgcgtc gcctatgcga
     1441 ccgtgctcgg acaaatgggc gaatatgccg aagcggccaa agtgcttggg accggcaagc
     1501 aggacgatta cagctacgcg gcacgcgctg cctacgccgc ccgcgcgaac gacaaaaccc
     1561 tgattgcgcc gctgtaccag gaactgctgg ccgacaagac gccgcgcaac ggcgcgc
//
LOCUS       DASRVO010000458         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_376560, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000458 DASRVO010000000
VERSION     DASRVO010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376560"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VFF05_05710"
     CDS             <1..661
                     /locus_tag="VFF05_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HZW51333.1"
                     /translation="LLPVYRGELQCRGLGLAVAILDDDGKPVRGEKGELACTAPFPCM
                     PVGFWNDPDGAKYKNAYFSKVENVWCHGDHALLTEHDGIVIYGRSDAVLNPGGVRIGT
                     AEIYRQVEHLPEVLESIAVGQEWQDDVRVILFVRLREGLTLDDTLRERIRKQIRDNTT
                     PRHVPAKILQVADIPRTISGKITEIAVRETIHGRPVKNTDALANPGALELFRDRTELR
                     S"
     gene            658..>1108
                     /locus_tag="VFF05_05715"
     CDS             658..>1108
                     /locus_tag="VFF05_05715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW51334.1"
                     /translation="MSATPDLTVTIPLYNEAENIPILYARVRDALDELGKRWELVLVD
                     DGSSDGSGALLDKIAADDARVVVLHFRRNYGQTAAFMAGIDHARGAIIIPMDGDLQND
                     PDDIGKLLAKLDEGFDVVSGWRKDRQDHAIKRNLPSRIANGLISRVSG"
BASE COUNT          216 a          357 c          349 g          186 t
ORIGIN      
        1 gctgctgccg gtgtatcgcg gcgaactgca atgccgtggg ctgggactcg cggttgcaat
       61 cctcgacgac gacggcaaac ccgtgcgtgg cgaaaaaggc gaactcgcct gtactgcgcc
      121 gtttccgtgc atgccggtcg ggttctggaa cgaccccgac ggcgccaagt ataaaaatgc
      181 gtatttttcc aaggtggaaa acgtctggtg ccacggcgac catgccctgc tgaccgagca
      241 cgacggcatc gtcatctacg gccgcagcga cgcggttctc aatcccggcg gcgtgcgcat
      301 cggcacggcg gaaatctacc gtcaggtcga gcacctgccc gaggtgcttg aatccattgc
      361 cgtcggccag gaatggcagg acgacgtgcg cgtgatcctg ttcgtgcgcc tgcgcgaggg
      421 cctcacgctc gacgataccc tgcgcgagcg catccgcaag cagatccgcg acaacaccac
      481 gccgcgccat gtgccggcaa aaatcctgca agtcgccgac atcccgcgca cgatttccgg
      541 caagatcacc gaaatcgctg tgcgcgagac gatccacggc cggccggtga agaacaccga
      601 tgcgctggcc aatccgggag cgctggagtt gttcagagac cggacggagt tgcgcagttg
      661 agcgccacgc ccgacctgac cgtcacgatt ccgctttaca acgaggcgga gaacataccg
      721 attctgtatg cgcgggtgcg cgacgccctc gatgagttgg gcaagcgctg ggaactcgtg
      781 ctcgtcgacg atggcagcag cgacggttcg ggcgcgctgc tcgacaagat tgccgcggac
      841 gatgcgcgtg tcgtcgtcct gcattttcgc cgcaattacg gccagaccgc cgccttcatg
      901 gccggcatcg accacgcgcg cggcgccatc attattccga tggacggcga cctacagaac
      961 gatccggacg acatcggcaa gctgctggcc aagctcgacg aaggtttcga tgtcgtctcc
     1021 ggctggcgca aggaccggca ggatcacgcg atcaagcgca acctgcccag ccgtatcgcg
     1081 aacggattga tctcgcgcgt atccggcg
//
LOCUS       DASRVO010000459         1088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_40170, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000459 DASRVO010000000
VERSION     DASRVO010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40170"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="VFF05_05720"
     CDS             <1..998
                     /locus_tag="VFF05_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZW51335.1"
                     /translation="IFSTTIFGGVSMGPQVDALRRGVDVLVATPGRLIDHMERRTLDL
                     SGIEILVLDEADRMLDMGFLPAIRRILGVLPKNRQTLLFSATFADEIKKIAGQFMRDP
                     AEIQVAARNSVVATITHRVHPVDATRKRDLLLHLLSEDSRRQTLVFGRTKHGADKLCK
                     FLDQSGLRTAAIHGNKSQNARTKALKEFKSGKITVLVATDIAARGLDIDQLPIVINYD
                     LPMVAEDYIHRIGRTGRAGAEGLAVSLVAHEEARLLRDIRRLLKQDIAIENVTGYEPS
                     RPLNLESPGPAPGRPGKPRNPNHARRPHAQPQNKPHNAANKKPRFWQGKRDARKA"
BASE COUNT          214 a          388 c          332 g          154 t
ORIGIN      
        1 gcattttcag caccacgatc ttcggtggcg tgagcatggg tccgcaggtg gacgcgctgc
       61 gtcgcggcgt cgacgtgctc gtcgccacgc ccggccgcct gatcgaccac atggagcggc
      121 gcacgctgga cttgtccggc atcgagatcc tggtgctcga cgaagccgac cgcatgctcg
      181 acatgggctt cctgcccgcg atccggcgca tcctcggcgt gctgccgaag aaccgccaga
      241 ccctgctgtt ctcggccacg ttcgccgacg agatcaagaa gatcgccggg cagttcatgc
      301 gcgatccggc cgaaatccag gttgccgcac gcaacagcgt ggttgccacg atcacgcatc
      361 gcgtgcatcc ggtcgatgcc acacgcaagc gcgacctgtt gttgcacctg ctcagcgaag
      421 acagccgccg tcagaccctg gtgttcggcc gcaccaagca tggcgccgac aagctgtgca
      481 agttcctgga tcaatccggc ctgcgcactg ccgcgatcca cggcaacaag agccagaacg
      541 cgcgcaccaa ggcgctgaag gaattcaaga gcggcaagat caccgtgctc gtcgccaccg
      601 acatcgccgc acgcggtctc gacatcgacc agttgccgat cgtgatcaat tacgacctgc
      661 cgatggttgc cgaggactac atccaccgca tcggtcgcac cggtcgcgct ggtgcggagg
      721 gcctggccgt gtcgctggtt gcgcacgagg aagcgcgcct gctgcgcgat atccgccgcc
      781 tgctcaagca ggacatcgcc atcgagaacg tgaccggata cgaaccgtcg cgcccgctca
      841 acctggaatc gccgggaccc gcgcccggca ggcccggcaa gccgcgcaac ccgaaccacg
      901 cacgccgtcc gcacgcgcag ccgcagaaca agccgcacaa cgcggccaac aagaagccgc
      961 gtttctggca gggcaaacgc gacgcgcgca aggcgtaaga atcagccggc ggcgccaatc
     1021 tccagcgccg ccgtgcaacc cttcaggaac ggtccgagat agcaatgcgt gccgcaggca
     1081 accagccc
//
LOCUS       DASRVO010000460         1943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_325395, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000460 DASRVO010000000
VERSION     DASRVO010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325395"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFF05_05725"
     CDS             complement(<1..700)
                     /locus_tag="VFF05_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51336.1"
                     /translation="MATEAPAFAFVFVCHGGELEIKSLLLAASLREAFGDAPQLIAAV
                     PQPAATWGAPSSAALEMFRRLCVDVQAVENPIGAEYPIGNKLACLSVATRARTVAVLD
                     SDILCLRGFDNDPAAATGFAAKPADKRTYPGGEAVWQPLYAAAGTTLPESRIATTLTG
                     EVGPPYFNSGVVFTTQPSRLGTAWIECARTLNPLLEGRGQRHWLDQVSLPIAVRHCGL
                     DYACLDDDFNFPAHL"
     gene            complement(690..>1943)
                     /locus_tag="VFF05_05730"
     CDS             complement(690..>1943)
                     /locus_tag="VFF05_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HZW51337.1"
                     /translation="PDPLQNELLQPGALSSMPTSAYSHWDPELPNRNLIPTFQYAAPE
                     GQGASDNGFVAVKPGSALPQLAVGRFPVVEPAEVTAIVDKTVAYLEHRNTGAWQRDVT
                     LISTSEVASFKGDSDKIAANLGRRGFVVDNVYTAFEEKDAARYQDARKILKHDLDKGN
                     VLVHFLGHGGQFIWRVGPIGDLFTLDDVDALTNSGMYPMVLSMTCFSAPFDHPTEDSI
                     GERFLRDPGKGAIAFFGASWMNWPDPGNSRVLIESLLTPGETIGDAILAVKHKAADPV
                     FVQMYNLLGDPAVTLALPQNALRMGVDRGRWETRVNVGIPASDFGGRVDVDWLDKNGD
                     RLVSAHYEARDVQFALTPPDKAVRVQVYAADSRNGLTAFGSADLTPPPVPVVKPKPKP
                     APHFVPRPRAPPAPDTIKRGDFDGD"
BASE COUNT          334 a          625 c          656 g          328 t
ORIGIN      
        1 tcaggtgcgc gggaaaattg aaatcgtcgt cgagacaggc gtagtcgagt ccgcagtggc
       61 gcacggcgat cggcagggat acctgatcca gccagtgtcg ctggccgcgc ccttcgagca
      121 agggattgag tgtgcgtgcg cattcgatcc atgccgtacc cagccgcgac ggttgcgtcg
      181 tgaacacgac gccgctattg aaatacggcg gcccgacttc cccggtcagc gtggtcgcga
      241 tgcgggattc cggcaacgtg gtgcccgcag cggcatacag cggctgccag acagcctcgc
      301 cgccgggata cgtgcgcttg tccgccggct tggcggcgaa gccggtcgcg gcggcaggat
      361 cgttgtcgaa tccgcgcagg cacaaaatgt cgctgtccag caccgcgacg gtccgcgcgc
      421 gcgtggccac gctcaggcag gcgagcttgt tgccgatcgg atattccgca ccgatgggat
      481 tttccaccgc ctgcacgtcc acgcacagac ggcggaacat ttccagcgcg gcggacgatg
      541 gcgcgcccca cgtcgccgcc ggctgcggca cggcggcgat gagttgcggt gcatcgccga
      601 atgcttcgcg cagcgaggcg gcgagcagca gcgacttgat ctccagttcg ccaccgtggc
      661 agacgaatac gaaggcgaac gcgggcgcct cagtcgccat cgaaatcgcc gcgtttgatc
      721 gtgtccggcg ccggcggcgc gcgtggccgc ggcacgaagt gcggcgcggg tttcggtttg
      781 ggcttgacca ccggcacagg cggcggcgtc aggtcggcgc tgccgaacgc ggtcaggcca
      841 ttgcgcgaat cggcggcgta cacctgcacg cgcacggctt tgtccggcgg cgtcagcgcg
      901 aattgcacat cgcgcgcttc gtaatgcgcg gagacaaggc gatcgccatt cttgtccagc
      961 cagtcgacat cgacgcggcc gccgaaatcc gacgccggaa tgcccacgtt cacgcgcgtt
     1021 tcccaacgcc cgcgatcgac gcccatgcgc aaggcattct gcggcagcgc cagcgtgacg
     1081 gccggatcgc cgagcaggtt gtacatctgc acgaacacgg gatcggcggc cttgtgcttc
     1141 accgccagga tggcatcgcc gatcgtctcg cccggcgtga gcaagctttc gatcaatacc
     1201 cgggaattgc ccgggtcggg ccagttcatc cacgacgcgc cgaaaaatgc gatcgcgccc
     1261 ttgcccggat cgcgcaggaa gcgctcgccg atggaatcct cggtgggatg atcgaatggc
     1321 gccgagaaac acgtcatcga cagcaccatc ggatacatgc cggaattggt cagcgcgtcg
     1381 acatcgtcga gggtgaacag gtcgccgatc ggaccgacgc gccagatgaa ctggccgccg
     1441 tggccgagga aatgcacgag cacgttgccc ttgtccaaat cgtgcttgag gatcttgcgc
     1501 gcatcctggt agcgcgccgc gtccttttcc tcgaacgcgg tgtagacgtt gtcgacgacg
     1561 aaaccgcggc gcccgagatt cgcggcaatc ttgtccgagt cgcccttgaa cgaggcgacc
     1621 tcgctggtgc tgatcagggt gacgtcgcgc tgccatgcgc ccgtgttgcg gtgttccaga
     1681 taggcgacgg tcttgtcgac gatggcggtg acttccgccg gctcgaccac gggaaaacgt
     1741 ccgaccgcca gttgcggcag ggcactgccg ggcttcaccg cgacgaagcc gttgtcgctg
     1801 gcaccctggc cttcgggcgc ggcgtattga aacgtcggga tcaaattgcg gttcggcaat
     1861 tccggatccc agtgcgaata cgcgctggtc ggcatgctcg acagggcgcc cggctgcagc
     1921 agttcgttct gcagcggatc ggg
//
LOCUS       DASRVO010000461         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_65452, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000461 DASRVO010000000
VERSION     DASRVO010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65452"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1040
                     /locus_tag="VFF05_05735"
     CDS             <1..>1040
                     /locus_tag="VFF05_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51338.1"
                     /translation="PINFTAVAYIVQRMESLCQLFVLAGLWGYIAARRRMLAGKPGLA
                     RAAAAIVLGTAIGGLAKESAALLPLYALIVEWIVFGFARTDGKTDRRLLGMYLVVLAL
                     PACATLYWVASHALPAAEWVNRPFSLGERLLTEPRILWDYVHWSLLPMANQLALYHDH
                     IRVSRGLLDPPTTLLAIAGLCALAIAAIALRRTHPLAALGILWFLAAHLLTATVIPLE
                     LVFEHRNYFASIGLYLAAFSFLLPAADARLTLARTALCLLVPVFFASVTWVRALNWRN
                     PILFALSEAQLNPDSPRTAYELAHTYVELSGYQADSPLVPKAYAALERAAALPGADTL
                     PDQGLLILSGRL"
BASE COUNT          142 a          380 c          316 g          202 t
ORIGIN      
        1 cgccgatcaa cttcacggcc gtcgcctaca tcgtgcagcg catggaaagc ctgtgccagc
       61 ttttcgtgct ggcgggattg tggggctaca ttgccgcgcg ccggcgcatg ctggccggca
      121 aacccggatt ggcgcgcgcc gcggcagcca tcgtgctcgg caccgccatc ggcggactcg
      181 ccaaggaatc ggccgcactg ttgccgctgt atgcgctgat cgtcgaatgg atcgtgttcg
      241 ggttcgcgcg caccgacggc aagaccgacc gccgcctgct ggggatgtac ctcgtcgtgc
      301 tggcgctgcc cgcctgcgcc acgctgtatt gggtcgccag tcacgcattg cccgccgccg
      361 agtgggtcaa ccggccattt tcgctgggtg agcgcctgct caccgaaccg cggatcttgt
      421 gggactacgt gcactggtcg ctgctgccaa tggcgaatca actcgcgctc taccacgacc
      481 acatccgcgt ctcgcgggga ctgctcgatc cgccgacgac actgctcgcc atcgcgggcc
      541 tttgcgcgct cgcgatcgcg gccatcgccc tgcgccgcac gcatccgctg gctgcactcg
      601 gcatcctctg gtttctcgcc gcgcacttgc tcactgcaac cgtgattccg ctggaactcg
      661 tcttcgaaca ccgcaactac ttcgccagta tcggcctgta cctcgccgcg ttttccttct
      721 tgctgccagc ggccgatgcg cgactgacgc tggcgcgcac ggcgttgtgc ctgctcgtgc
      781 cggtgttctt cgccagcgtc acctgggtgc gcgcgctgaa ctggcgcaat ccgatcctgt
      841 tcgcgctcag cgaagcgcag ctgaatcccg attcgccgcg caccgcctac gaactggcgc
      901 acacctacgt cgagctttcc ggataccagg ccgattcgcc gctggttccg aaagcctatg
      961 ccgcgctcga acgcgccgcc gccttgccgg gcgccgacac cttgccggac cagggactgc
     1021 tgatcctgtc cgggcgcctg
//
LOCUS       DASRVO010000462         1952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_65456, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000462 DASRVO010000000
VERSION     DASRVO010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65456"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFF05_05740"
     CDS             complement(<1..331)
                     /locus_tag="VFF05_05740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161761.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZW51339.1"
                     /translation="MATTLNPSEISELIRTRIEKVKLAAEARNEGTIVSVSDGIIRIH
                     GLADVMSGEMIELPNNGTALALNLERDSVGAVVLGEYEYLTEGDTVKTTGRILEVPIG
                     PELLGRVV"
     gene            complement(453..986)
                     /locus_tag="VFF05_05745"
     CDS             complement(453..986)
                     /locus_tag="VFF05_05745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZW51340.1"
                     /translation="MSEPLTLARPYARAAFETARTHNALAAWSDNMRFAAQVMADARV
                     STVAGDPRLSHAELVGLLLPPGEAADAPFATFLSLLVENRRVNLLAEIAALFEELKRE
                     SERTIRVILRSASEVPADRADAIKVALKKRFAREIELEQRIDPAVIGGAVIDAGDVVI
                     DGSVRGRLERLQTALAE"
     gene            complement(989..1459)
                     /locus_tag="VFF05_05750"
     CDS             complement(989..1459)
                     /locus_tag="VFF05_05750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZW51341.1"
                     /translation="MNINATLIAQILVFGILIWVTMKYLWPPISQAMDERARKIADGL
                     SAAERAQAELKDADARVAEEIRKARVQATEIVDKAHAQANQIVDKAKTDALLEAARVK
                     ATAQADIDGMVGKAREALRGQVASLAVQGASKIVQREIDPAAHKALLDQLVAEI"
     gene            complement(1491..1784)
                     /gene="atpE"
                     /locus_tag="VFF05_05755"
     CDS             complement(1491..1784)
                     /gene="atpE"
                     /locus_tag="VFF05_05755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009834415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZW51342.1"
                     /translation="MDISALASVEGLTALAAGLMLGLGGIGAAIGMGLMGGKFLEGAS
                     RQPELVPMLQGKMFLLVGLIDAIPIIGVGIALYLIFVDPFSGKLLAQAAGAAH"
     gene            complement(1825..>1952)
                     /locus_tag="VFF05_05760"
     CDS             complement(1825..>1952)
                     /locus_tag="VFF05_05760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216916.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit A is part of the
                     membrane proton channel F0; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZW51343.1"
                     /translation="AYYQILFAAAWTLFHVLIISLQAYIFMVLTVVYMAMAADHH"
BASE COUNT          323 a          616 c          648 g          365 t
ORIGIN      
        1 cgaccacgcg gccaagcagt tccgggccga tcggcacttc gagaatgcgg ccagtggtct
       61 tgaccgtatc gccttcggtc aggtactcgt actcgccgag cacgaccgcg ccgaccgaat
      121 cgcgctcgag gttcagcgcc agcgccgtgc cgttgttcgg cagttcgatc atttcgcccg
      181 acatcacgtc ggcgaggccg tggatgcgga tgatgccgtc ggacaccgag acgatggtgc
      241 cttcgttgcg cgcttcggcg gcgagcttca ccttttcgat gcgggtgcgg atcagttcgc
      301 tgatttcgga cggattgaga gtcgttgcca tgctggtttc ctcgagcgcg gacgcgaacg
      361 cccgtgcctg tggttgaagt caaaaatcaa aagtcagggg ccattggccg ctcacgtttg
      421 atttttccgc ggcgcgtgcg gcgccgcggg cgtcactccg ccaatgcggt ctgcaaacgt
      481 tccaggcggc cgcgcaccga accgtcgatc accacgtcgc cggcgtcgat caccgcaccg
      541 ccgattaccg ccggatcgat gcgttgttcg agttcgatct cgcgcgcgaa acgctttttc
      601 agcgcgacct tgatcgcgtc ggcacgatct gcgggaacct cgctggcgct gcgcaagatt
      661 acgcggatcg tgcgttcgga ttcgcgcttg agttcctcga acaacgcggc gatttcggcg
      721 agcaggttca cgcgccggtt ttcgacgagc aggctcagga atgtcgcgaa cggcgcatcc
      781 gcggcctcgc ccggcggcag caacaggccg acgagttcgg cgtgggacag gcgcgggtcg
      841 cccgcgaccg tactcacgcg cgcatcggcc atcacctgcg cggcgaagcg catgttgtcc
      901 gaccaggcgg cgagcgcgtt gtgtgtgcgc gccgtttcga acgcggcgcg cgcatacgga
      961 cgggcgaggg tcagcggttc gctcatcgtc agatctcggc aacgagctga tcgagcagcg
     1021 ccttgtgcgc cgccggatcg atctcgcgct gcacgatttt cgacgcacct tgcacggcga
     1081 gcgacgcgac ctggccgcgc agcgcttcgc gcgccttgcc gaccatgccg tcgatatcgg
     1141 cctgcgccgt ggccttcacg cgcgcggctt cgagcagcgc gtcggtcttg gccttgtcga
     1201 cgatctggtt cgcctgcgcg tgcgccttgt cgacgatttc cgtcgcctgc acgcgcgcct
     1261 tcctgatttc ctcggccacg cgggcgtcgg cgtccttgag ttccgcctgc gcgcgttccg
     1321 ccgcggacag gccgtcggcg atcttgcgtg cacgttcgtc catggcctgg gaaatcggcg
     1381 gccacaggta cttcatcgtg acccagatca ggatcccgaa gacgaggatc tgggcgatga
     1441 gggttgcgtt gatgttcacg ggatgcttcc tcgacgcgaa gaggaacggg ttagtgcgcc
     1501 gcgcccgccg cctgtgcgag cagcttgccg gagaacggat cgacgaagat caggtacagc
     1561 gcgataccca cgccgatgat cggaatcgcg tcgatcaggc cgaccagcag gaacatcttg
     1621 ccctgcagca tcggcacgag ttcgggctgg cgcgaggcgc cttcgaggaa cttgccgccc
     1681 atcaggccca tgccgatggc ggcgccgatg ccgccgaggc cgagcatcag gccggccgcc
     1741 agggcggtca ggccttcaac ggaagcgagt gcgctgatat ccatgtgtat ctccgagtgt
     1801 gttgcggttg agtgggaagg tgaatcagtg atgatcggcc gccatcgcca tgtagacgac
     1861 ggtcagcacc atgaagatgt aggcctgcag cgaaatgatc aggacgtgga acaaggtcca
     1921 tgctgcagcg aaaagaattt gataatacgc ag
//
LOCUS       DASRVO010000463         1102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_221537, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000463 DASRVO010000000
VERSION     DASRVO010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221537"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VFF05_05765"
     CDS             <1..1078
                     /locus_tag="VFF05_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZW51344.1"
                     /translation="ALEGGVAALAVASGQAASLFCVNNIAQTGDNFVASTDLYGGTWN
                     LFLHTMQRTMGIEVRFVDPADPENFRRATDAKTRCYYAETLPNPKLEVFPIAEVAKIG
                     RELGVPLIMDNTAAPVICRPLDHGAAVVMHSTTKYIGGHGTSIGGIVVDGGNFDWEKH
                     ADRFPLLNQPDPAYHGAVWTQAVKPLGPIAYILKMRVTLLRDIGAAASPFNDFLHIQG
                     LESLPLRMRAHCTNAAAVAEFLKGQPKVAKVIYPSLMEGAARRRADAHLKGGYGGLLG
                     FELKGGIAAGRRFIDALEMFYHVANIGDARSLAIHPASTTHSQLTPEEQLATGVTDGY
                     VRLSLGIEHPDDILADLKQALDKA"
BASE COUNT          198 a          346 c          364 g          194 t
ORIGIN      
        1 cgcgctggag ggcggggtcg cggcgctggc ggtggcttcg gggcaggctg cctcgctgtt
       61 ctgcgtcaac aacatcgccc agacgggcga caatttcgtc gcttcgaccg atctttacgg
      121 cggcacctgg aacctgttcc tgcacacgat gcagcgcacg atgggaatcg aggtgcggtt
      181 tgtcgatccg gctgacccgg agaatttccg ccgcgccacc gacgccaaga cgcgctgcta
      241 ctacgcggaa accttgccca acccgaagct cgaagtgttt ccgatcgccg aggtcgcgaa
      301 gatcggccgc gagctgggcg tgccgctgat catggataac accgcagcgc cggtgatctg
      361 ccggccgctc gatcacggcg ccgcggtcgt tatgcactcg acgaccaaat acatcggcgg
      421 ccacggcacc tcaatcggcg gtatcgtcgt cgatggcggc aatttcgatt gggagaagca
      481 tgccgaccgc tttccgctgc tcaaccagcc ggatcccgct tatcacggcg cggtgtggac
      541 gcaggcggtg aagcccttgg ggccgattgc ttacatcctc aagatgcggg tgacgttgct
      601 gcgtgacatc ggcgccgcgg caagcccgtt taacgatttt ctgcatatcc agggattgga
      661 aagcctgccg ctgcgcatgc gggcgcactg cacaaatgcc gcagcggtcg cggaattcct
      721 gaagggtcag ccgaaggttg ccaaggtgat ttacccgagc ctgatggagg gcgcggcgcg
      781 ccggcgcgcc gatgcgcatc tgaagggtgg ctatggcgga ttgctcgggt tcgagctgaa
      841 aggtggcatt gccgccgggc ggcgctttat cgatgcgctg gagatgttct accacgtcgc
      901 caatatcggc gatgcgcgca gcctggcgat ccacccggct tcgacgacgc actcgcaact
      961 gacgccggaa gagcaactgg cgaccggcgt caccgacggc tatgtgcgcc tgtcgctcgg
     1021 catcgagcac ccggacgaca tcctcgccga cttgaagcaa gcactcgaca aggcgtgatg
     1081 gcggaagcgg cccgaccgcc ga
//
LOCUS       DASRVO010000464         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_195656, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000464 DASRVO010000000
VERSION     DASRVO010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195656"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..840
                     /locus_tag="VFF05_05770"
     CDS             61..840
                     /locus_tag="VFF05_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51345.1"
                     /translation="MKILISCVASALALSAFSGAFAAEASKANVYRDFSDTVAPANQE
                     AYEAGVKAYNQCMKDHGFKYTWGAWNHETGNVYTYSYVAGPYTWADFDTMHTTGKACD
                     ATWRKQANPHLKEETSAFMVSQADMSYMPKDADKDPPPAMIGVVYFHLKNGHDAHEAF
                     TSAVKKIAAGAAKSNWSGHFQTDEINYGGEGAPNYIVVFPNKNWADVGMEANPSLWKM
                     MENVYGKAEAAAIRKSLNDSIEKSSSHIDSYNADLTYTAPK"
     gene            919..1470
                     /locus_tag="VFF05_05775"
     CDS             919..1470
                     /locus_tag="VFF05_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HZW51346.1"
                     /translation="MSANLVSPTPRAAPTRRMLRWLLGGTLALAATVAAAAPTAQEYI
                     SGFVLYVRWPEDAQIKTWQVCVAATSTAGDAHYADLRVRDRPFVLRHVKSGDDLDGCQ
                     VLDLTAADPATSANLLKATQSRSMLTVGSGRDFCSTGGMICLLLDQPRGGFEVNLSAI
                     RGAGLLINAKLLMMARQSGSAAP"
     gene            1467..>2141
                     /locus_tag="VFF05_05780"
     CDS             1467..>2141
                     /locus_tag="VFF05_05780"
                     /inference="COORDINATES: protein motif:HMM:NF028461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HZW51347.1"
                     /translation="MTATPERRTLAEHIGRAQLMVYAAAVVLATVGASIVGYHYLHQQ
                     TLAHLQNLARITAIESQAGLVFGDDSASADVLRAIPAEEGVTRAELFDAQGRLRASVA
                     NAPSGPIGALAAHFDGEQVSAPVIADKRMIGEVRLSGGEEPLLNAFLGLILADIVVVT
                     CIGILSLFVSRRNTRRITRPLTQLRTLMHEATQQQDFTQRAPAADIAEVDDLRVQFNG
                     LLDEIVQ"
BASE COUNT          419 a          716 c          656 g          350 t
ORIGIN      
        1 ccccgctcgc aacaacgggt cgacacgctg tctcatcaac taccaaggga ggaaggtttc
       61 atgaaaatcc tgatctcgtg cgtggcgtcg gcgctggccc tttcggcctt ctccggcgcg
      121 tttgcggcag aggcaagcaa ggcgaatgtc tatcgtgatt tcagcgacac cgtggctccc
      181 gccaatcagg aagcctacga ggccggcgtc aaagcctaca accagtgcat gaaggaccac
      241 gggttcaagt acacctgggg cgcgtggaac catgaaaccg gaaacgtcta cacatactcg
      301 tatgtggccg gaccttatac ttgggccgac ttcgacacga tgcacaccac gggcaaggct
      361 tgcgatgcga cctggcgcaa acaggccaac ccgcacctga aggaagaaac cagtgcgttc
      421 atggtctcgc aggcggacat gagttacatg ccaaaggatg ccgacaaaga cccgccgccg
      481 gcgatgattg gcgtcgtcta ttttcatctc aagaacggcc acgacgcgca cgaagcgttc
      541 acgagcgcgg tgaagaagat tgccgccggg gcggccaaat ccaattggtc gggccatttc
      601 cagaccgacg aaatcaacta tggcggcgaa ggcgcgccga attacatcgt cgtctttccg
      661 aacaagaact gggcggatgt cggcatggaa gccaatccgt cgctgtggaa gatgatggag
      721 aacgtctacg gcaaggccga agccgccgca atccgcaaat cgctcaacga ttccatcgaa
      781 aaatcctcct cgcacatcga cagctacaac gccgacctga cctacaccgc acccaagtga
      841 ttcacgtcgc gccacggtgc gcttcggcgc atcgcggcgc tttttccacg tcgccatgcc
      901 gcttgctatc ctagccgcat gtccgcgaac ctggtgtcgc caactccgcg cgcagcgcca
      961 acgcggcgca tgctgcgctg gctgctcggc ggcacgctgg cgctggcggc gactgtcgcg
     1021 gcagcggcgc cgaccgcaca ggaatacatc agcggcttcg tgctctacgt gcgctggccg
     1081 gaagacgcgc aaatcaagac ctggcaggtg tgcgtggccg cgacgagcac ggcgggcgat
     1141 gcgcactacg cagacctgcg ggtgcgcgac cggccgttcg tgctgcgcca tgtgaaatcc
     1201 ggcgacgacc tggatggctg ccaggtgctc gacctgactg ccgcggaccc cgccacttcg
     1261 gcgaacctgc tcaaggccac gcaatcgcgc agcatgctga ccgtgggcag cggccgcgac
     1321 ttctgcagta ccggcggcat gatctgcctg ctgctggacc agccgcgcgg cggcttcgaa
     1381 gtgaacctga gcgccatccg cggcgccggt ctcctgatta acgccaaatt gctgatgatg
     1441 gcgcgccagt ccgggagcgc ggcgccatga ccgctacgcc cgaacgccgc accctggccg
     1501 aacacatcgg ccgcgcgcag ctcatggtgt atgccgccgc cgtggtactt gccaccgtcg
     1561 gcgcctcgat cgtcggctat cactacctgc accagcaaac gctcgcgcat ctgcaaaacc
     1621 tcgcgcggat aacggcgatc gagagccagg ccggcctcgt gttcggcgac gattccgcca
     1681 gcgcggacgt gttgcgcgcg attccggccg aggaaggtgt gacccgcgcc gaactgttcg
     1741 acgcgcaggg cagattgcgc gcaagcgtcg ccaatgcgcc ctccggaccg atcggcgcgc
     1801 tcgccgcgca tttcgacggc gaacaggtca gcgccccggt gatcgccgac aagcgcatga
     1861 tcggcgaggt gcgcctgagt ggcggcgaag aacctttgct caacgctttt ctcggcttga
     1921 tcctggccga catcgtggtg gtcacctgca tcggcatcct gtcgctgttc gtctcgcgcc
     1981 gcaacacgcg tcgcatcacg cgtccgctga cccaactgcg cacgctgatg cacgaggcca
     2041 cgcagcagca ggatttcacg cagcgcgcgc cagccgcgga catcgccgag gtcgacgacc
     2101 tgcgcgtgca gttcaacggc ctgctcgacg aaatcgtgca g
//
LOCUS       DASRVO010000465         1042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_195665, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000465 DASRVO010000000
VERSION     DASRVO010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195665"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFF05_05785"
     CDS             complement(<1..828)
                     /locus_tag="VFF05_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51348.1"
                     /translation="MKRFFACLAFCCAAGAALADPVYDTQEFRAYENDGSIAYRAGNY
                     AQAEQAYASAALYGPQQGGAFYNLAAAAARAGDREKALASLDRFASFGLAVNLDSDSA
                     FASLRGLAEFARIRGKLAANTAPICACTTIFQGTAEPFIAEGITQDIRTGRIFVSSVV
                     ERKIVAIKDGKARDFAKLPDGMSALGIRINDAHYLIWAAASTLPQSDGGSGDMGNAAL
                     LAFDITSGALRGKYRAPVYAPRRSFSDLTFAPDGTVYVSDAIEGSIFRLKPGDDTLEV
                     "
BASE COUNT          189 a          318 c          344 g          191 t
ORIGIN      
        1 cacttccagc gtatcgtcgc cgggcttcag gcggaagatc gaaccttcga tcgcatcgct
       61 gacatagacg gttccatcgg gcgcgaaggt gaggtcgctg aaggagcggc gcggcgcgta
      121 gaccggcgcg cgatatttgc cgcgcagcgc gccgctggtg atgtcgaagg cgagcaatgc
      181 ggcatttccc atgtcgccgg atccgccgtc gctttgcggc aatgttgagg cggcagccca
      241 gatcagataa tgcgcgtcgt tgatgcggat gccgagcgcg gacatgccgt ccggcagctt
      301 cgcgaagtcg cgggccttgc cgtccttgat cgccacgatc ttccgctcca ccacgctgga
      361 cacgaagatg cggccggtgc ggatgtcctg cgtgatgcct tcggcgataa agggctccgc
      421 ggtcccctgg aatatggttg tgcaggcgca gatcggtgcg gtgttcgccg caagctttcc
      481 acgaatgcgc gcaaattcgg ccaggcctcg cagcgatgcg aaggcactgt cgctgtccag
      541 attcaccgcg aggccgaagc ttgcaaagcg atccagagag gcgagcgcct tctcccggtc
      601 gccggcacgc gcggcggcgg ccgcgagatt gtagaaggcc ccgccctgct gcggaccgta
      661 gagcgccgcc gatgcgtagg cctgttcggc ttgcgcataa ttgccggcgc gataggcgat
      721 cgatccgtcg ttttcatagg cgcggaattc ctgggtgtcg taaaccggat cggccagcgc
      781 ggcgccggct gcgcaacaga acgccagaca cgcgaaaaac cgcttcattc cgaaccccga
      841 acgttggccg acaataatgt gggcgcgcga aaatcccagc cacatcgccg cgattgaatt
      901 tctgttccta gaatgttctg atggccggat gaacggacat acggctcagg atgtggcgcg
      961 cggggtgtcg cgcctgctca tgcaggaagg ctattcgccg atcctggaat tcacgctcgc
     1021 caatgggcga cggctcgatg tc
//
LOCUS       DASRVO010000466         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_350506, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000466 DASRVO010000000
VERSION     DASRVO010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350506"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VFF05_05790"
     CDS             complement(<1..167)
                     /locus_tag="VFF05_05790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="catalyzes the transcription of DNA into RNA using
                     the four ribonucleoside triphosphates as substrates.
                     Dimerization of the alpha subunit is the first step in the
                     sequential assembly of subunits to form the holoenzyme;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZW51349.1"
                     /translation="MANSPTTILRPRGLHVERTGVNRARVVVEPLERGFGHTLGNALR
                     RVLLSSIPGAAV"
     gene            complement(329..970)
                     /gene="rpsD"
                     /locus_tag="VFF05_05795"
     CDS             complement(329..970)
                     /gene="rpsD"
                     /locus_tag="VFF05_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZW51350.1"
                     /translation="MARYIGPKLKLSCREGADLSLKSPARAIESKCKKFEKLQKPGQH
                     GAAAGKKGRLSDYAVQLREKQKVKRIYGLLERQFSNYYSKASQLKGNTGENLLRMLEA
                     RLDNIVYRMGFAVTRAQARQLVSHKAIEVNGKKVNLPSYQVRAGDQVAVVEKSRNQLR
                     ITEALNLSQQMDLAPSWIEVDAKKFAGSLKALPERSDLPSDINESLIVELYSK"
     gene            complement(973..1368)
                     /gene="rpsK"
                     /locus_tag="VFF05_05800"
     CDS             complement(973..1368)
                     /gene="rpsK"
                     /locus_tag="VFF05_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017939146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZW51351.1"
                     /translation="MNKPVAAVKTKKKAKRVVTDGVVHVQASFNNTVVTITDRQGNAL
                     SWATAGGAGFRGSRKSTPFAAQVAAEKAGRVAAEYGVKTVEVRIKGPGPGRESAVRSL
                     NALGIKVTNIIDVTPIPHNGCRPPKRRRV"
     gene            complement(1416..1775)
                     /gene="rpsM"
                     /locus_tag="VFF05_05805"
     CDS             complement(1416..1775)
                     /gene="rpsM"
                     /locus_tag="VFF05_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZW51352.1"
                     /translation="MARIAGVNLAVQKHVVIGLQSIFGIGRARSRQVCVAAGVDPATK
                     IKELTEGQVEKLRAEVAKYTVEGDLRRENGMAIKRLIDLGTYRGLRHRRGLPVRGQRT
                     RTNARTRKGPRRAIKKN"
     gene            complement(1890..>3103)
                     /gene="secY"
                     /locus_tag="VFF05_05810"
     CDS             complement(1890..>3103)
                     /gene="secY"
                     /locus_tag="VFF05_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZW51353.1"
                     /translation="GVNSDAMTKLIQGQGGGLVDMFNMFSGGALARFSLFALGVVPYI
                     SASIIVQMFGSVIPSWAAMRKEGESGRRKLTQYTRFGTIGLAAFQAFGIATMLQHQAG
                     VVYAPGPSFIFTAMVGLTAGTMFLMWVGEQITERGIGNGISLLIFAGIVAGLPSAVTR
                     TLEMSQQGEITPMKVLVILALVLLVTAFVVFMERGQRRITVNYARRQSGSRAYMNQSS
                     HLPLKINMSGVIPPIFASSLILFPATASSWFSNGADIRWLQQITAALNPGEPLYEILY
                     GAMIVIFAFFYTALVFNSSETADNLKKSGALVPGIRPGRSTAEYIDGVLTRLTGAGAI
                     YLVAVCLVPTLLQQNWHVPFYFGGTSLLIVVVVVMDFTAQVQAHLVSHQYESLLKKAN
                     LKGYGRGGSQR"
BASE COUNT          575 a          974 c          965 g          589 t
ORIGIN      
        1 accgccgcgc cgggaatcga cgacagcagc acgcgccgca gcgcgttgcc gagggtatgg
       61 ccgaaaccgc gttccagcgg ctcgacgacg acgcgtgcgc ggttgacacc ggtacgttcg
      121 acatgcaggc cgcgcgggcg caggatggta gtaggcgaat ttgccatgaa ctacgctctc
      181 cgaaagtgat tgatagggtg caaaaccggg catcctgccg cagcttgctt gcaaacggtc
      241 gcgacacggc cgcgccacgt ttgtcgcggc gggcacttgc gtgctcgccg cgccggcaca
      301 tcctgtgccg aagcgggtat tcgacaaatt acttcgagta aagctcgacg atcagcgatt
      361 cgttgatgtc gctgggcaga tccgagcgtt ccggcaacgc cttcagcgaa ccggcgaact
      421 tcttcgcatc gacttcgatc cagctcggcg cgagatccat ttgctgcgac aggttcaatg
      481 cttcggtgat gcgcaactgg ttacgcgact tctcgacgac ggcgacctgg tcgccggccc
      541 tgacctggta cgacggcaga ttgacctttt tgccgttgac ttcgatcgcc ttgtgcgaca
      601 ccagctggcg tgcctgcgcg cgcgtgacag cgaagcccat gcggtagacg atattgtcca
      661 aacgggcttc gagcatgcgc agcaggtttt cgccggtgtt gcccttgagc tgcgaagcct
      721 tgctgtagta attgctgaac tggcgctcga gcaagccata gatgcgcttg accttctgct
      781 tctcgcgcag ctgcacggca tagtcggaca ggcggccttt cttgcccgcc gccgcgccgt
      841 gctggccggg tttctgcagc ttctcgaatt tcttgcactt ggattcgatc gcgcgcgccg
      901 ggctcttgag gctgagatcg gcgccttcgc ggcaggacag cttgagtttg ggaccgatat
      961 aacgtgccat gattaaaccc tacgacgctt cggcggacgg cagccgttgt gtggaatcgg
     1021 cgtgacgtcg atgatgttgg tgaccttgat gccgagcgcg ttcagcgaac gcacggccga
     1081 ctcgcggccc ggacccggac ccttgatgcg cacttccacg gtcttgacgc catactcggc
     1141 agccacgcgt ccggccttct ccgccgcgac ctgggcggcg aatggagtcg acttgcgcga
     1201 accgcggaac cccgcgccgc cggcagttgc ccacgacagt gcgttgccct ggcgatcggt
     1261 gatcgtcacg acagtgttgt tgaacgaagc ctgcacatgc acgacgccgt cggtgacgac
     1321 gcgcttggct ttcttcttgg tctttacggc agcgaccggt ttattcattt gtcaggatgt
     1381 ccgcagaaat tcgatggaat cggtctggag ctaaatcagt tcttcttgat ggcgcggcgc
     1441 gggcccttgc gggtgcgagc gttcgtgcgc gtgcgctgac cgcgtaccgg caggccgcgg
     1501 cgatgacgca ggccgcgata ggtgccgagg tcgatcaggc gcttgatcgc catgccgttc
     1561 tcgcgacgca ggtcgccttc gaccgtgtac ttggcgactt cggcacgcag cttctcgacc
     1621 tggccttcgg tgagttcctt gatcttggtc gccggatcca cgcctgcggc cacgcacacc
     1681 tgcctggacc gtgcccggcc gatgccgaaa atgctctgca agccgatcac gacatgcttc
     1741 tgcaccgcca aattgacacc cgcaatacgc gccatcttgt tgtctccgaa agcggcttcc
     1801 gcgcaaaaat gcgcagtgaa ccgtgaatta taaccagatt tgtgctgtta tcaaagcgct
     1861 aaaaccgcgc ctcgccttca atccatgcct caacgctgac ttccaccgcg gccatagccc
     1921 ttcaggttgg ctttcttgag caggctttcg tactggtgcg acaccaagtg cgcctgcacc
     1981 tgcgcggtga aatccatgac cacgacgacc acgatcagca acgacgtgcc gccgaaatag
     2041 aacggcacat gccagttctg ctgcagcagg gtcgggacca ggcacaccgc gacgaggtag
     2101 atcgcgccgg cgccggtcag ccgggtcagc acgccgtcga tgtattcggc ggtagaccgt
     2161 cccggacgga tacccggcac caaggcgccg gatttcttca ggttgtccgc ggtttcgctg
     2221 gagttgaaca ccagcgccgt gtagaagaac gcgaagatga cgatcatcgc gccgtaaagg
     2281 atttcgtaca gcggctcgcc cggattcagc gccgcggtaa tctgctgcaa ccagcggatg
     2341 tccgcgccgt tggagaacca cgacgaggcg gtggccggga acaggatcaa cgacgaagcg
     2401 aagatcggcg ggatcacgcc ggacatgttg atcttcagcg gcaggtgcga gctctggttc
     2461 atgtacgcac gcgaaccgct ctggcgacgc gcgtagttca cggtgatgcg gcgctggccg
     2521 cgctccatga acaccacgaa cgcagtgacc agcagaacca gcgccagaat gaccagcacc
     2581 ttcatcggcg tgatttcgcc ctgctgcgac atttccagcg tgcgcgtcac cgcgctcggc
     2641 aagccggcga cgatgcctgc gaagatcagc agcgaaatgc cgttgccgat gccgcgctcg
     2701 gtgatctgct cgcctaccca catcaggaac atggtgccgg cggtgaggcc gaccatggcg
     2761 gtgaagatga agctcggacc gggcgcgtag accacgccgg cctgatgctg cagcatggtg
     2821 gcgatgccga aggcctgaaa cgcggccagg ccaatggtgc caaagcgcgt gtactgggtg
     2881 agtttgcgcc ggccggattc gccttccttg cgcatcgccg cccagctcgg gatcaccgaa
     2941 ccgaacatct gcacgatgat cgacgcggaa atgtacggca ccacgccgag cgcgaacagc
     3001 gagaagcgcg ccagtgcgcc gccggagaac atgttgaaca tgtccaccag gccgccgccc
     3061 tgaccctgga tcagcttggt catggcatcg gagttcacgc ccg
//
LOCUS       DASRVO010000467         1566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_117288, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000467 DASRVO010000000
VERSION     DASRVO010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117288"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFF05_05815"
     CDS             complement(<1..532)
                     /locus_tag="VFF05_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HZW51354.1"
                     /translation="MPLARVLCALLLAASGSALAAPYAYVPNEGSGSVSIIDTATDAV
                     VADIPAGKKPRGTAVSPDGRWLYVSDQPNNRLVVIDLQTRKQAASVPLGESPEGVGMS
                     PDGRWVSAAIEESNDVAFVDTRDNRLAFIVKVRGKNPEHAEFSPDGRLVFVSAEEGSA
                     VDIIDFAQRKEIAKVPV"
     gene            complement(540..1163)
                     /locus_tag="VFF05_05820"
     CDS             complement(540..1163)
                     /locus_tag="VFF05_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HZW51355.1"
                     /translation="MLVAGSAWGQAGLQNANSELLLAARSGRVATVTALLEQGANPNS
                     RNRIGDTPLNTAAKKGDAAIVKLMLERGADVNLQNASGVSPLMSAAFNGHADIVKLLL
                     AHKADPTSVDRIMKTAAIYAAGTGHADCLAALLDGGVGVDAVYANDLTALMWAAGSGQ
                     AATVKLLLARGADVTRRDNRGKTALAMALDKGDRETIDALRAAGAKE"
BASE COUNT          264 a          517 c          513 g          272 t
ORIGIN      
        1 cgaccgggac cttggcgatc tccttgcgct gggcgaagtc gatgatgtcg accgcgctgc
       61 cttcctcggc gctgacgaac accaggcgcc cgtccgggct gaattcggcg tgctccggat
      121 tcttgccgcg aaccttgacg atgaaggcga ggcggttgtc gcgcgtgtcg acgaacgcaa
      181 cgtcgttgct ttcctcgatc gcggcggaga cccagcgccc gtcgggcgac atgcctacgc
      241 cctcgggcga ttcgccgaga ggcacggacg cggcctgctt gcgcgtctgc aaatcgatca
      301 ccaccaggcg gttgttaggc tggtcgctca catagagcca acgaccgtcc gggctgaccg
      361 cagtgccgcg cggcttcttg ccggcgggga tatcggccac gaccgcgtcg gtcgccgtgt
      421 cgatgatgga cacgctgccc gagccctcgt tcggcacata ggcataaggg gccgcgagcg
      481 ccgaccccga cgcggcgagc aggagggcgc acaggacccg cgcaaggggc atggcgcggc
      541 tactctttcg cgccagccgc gcgcagcgcg tcgatcgttt cgcggtcgcc tttgtcgagc
      601 gccatggcga gcgcggtctt gccgcgattg tcccgcctgg tgacgtcggc gccccgcgcc
      661 aacagcagct tcacggtggc ggcctggccg gagcctgccg cccacatcag cgcggtcagg
      721 tcgttcgcgt acacggcatc gacgccgacg ccgccgtcca acagcgcggc caggcagtcc
      781 gcatggcccg tgcccgcggc gtagatggcg gcggtcttca tgatccggtc gaccgacgtc
      841 gggtcggcct tgtgcgcgag cagcagcttc acgatgtcgg cgtgcccgtt gaacgcggcg
      901 ctcatcaggg gcgagacccc ggaggcattc tgcaggttga cgtccgcgcc gcgctcgagc
      961 atgagtttta cgattgcggc atcgcccttc ttggcggccg tgttgagcgg cgtgtcgcca
     1021 atgcgattgc gcgagttggg gttcgcgccc tgctcgagca gggcggtcac cgtcgccacc
     1081 ctgccggaac gcgcggcgag cagcaattcg ctgttggcgt tctggaggcc ggcctgaccc
     1141 cacgcgcttc ccgcgaccaa caccagcccc aatacgccca gccgtctgaa acctctcatc
     1201 ttgcttaccc tcccagggtg cccgctgtcc ggcgcatgct gcgccgcagt atgggcgcga
     1261 cttccaaccg cacagaacgt ttgtgtctta tttcgcgttt agtatagagt aagtgccaag
     1321 aaaaataaca ttagaacaaa attcacaagg caccacttct tcgcttgacc gtggcgaaag
     1381 cctcaggatt cgcgtcaggc attgttctgg tttcacgatc acgacgcaac gagaagcgaa
     1441 acgaatacaa cacgagggag atgtcaatgt cgaaggaaca ccgattgatc ggaatcgccg
     1501 cggcagccgc cttgatggcg gcagcgggcg tggcgggcgc gcaggaaatc acgcgtcaca
     1561 aggcgg
//
LOCUS       DASRVO010000468         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_168435, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000468 DASRVO010000000
VERSION     DASRVO010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168435"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFF05_05825"
     CDS             <1..262
                     /locus_tag="VFF05_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HZW51356.1"
                     /translation="DNRHRELHGALLDAQLLADVYLAMTGGQGDLGLASAQAVAPARV
                     ATQFVARRPLRVRRADATEAALHAARLQAIDKASKGRCLWRA"
     gene            317..1630
                     /locus_tag="VFF05_05830"
     CDS             317..1630
                     /locus_tag="VFF05_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZW51357.1"
                     /translation="MNAMTTLLAGMLLCAFGAHAETAVEAIAAHKHMGVATCSSSVCH
                     GASQPFRDSDIAQNEFAVWQEFDPHAKAYQVLEQPRSQEIARKLGLGDATKAKVCLDC
                     HADNMPAGDRGERFQISDGVGCEACHGGAELWLNSHADRKVTYADDLAKGMYPTADPI
                     KRAQLCLSCHMGEKERMITHRIMGAGHPRLSFELDTFTWLNPHYKITANWIKRKGEWN
                     GVRDWAVGQGVAAENLLGLLLDPKTGWQGIFPELVLFNCQACHKLMKGNNWGPRQGTG
                     LGPGVVRLNDANLVTFRYALAPVDKTAAQRVFEQTRALHQATTQSRDATFAAARTLNA
                     TLESLLAKVAAYNYDAASLNPILAAMLADAQQGEFRDYAAAEQASMAAQSLVVAFEND
                     GKLDKAHAAALHTKLDGLYAAVKDEDRYSMAPFVAALKDLRAAAP"
     gene            1641..3062
                     /locus_tag="VFF05_05835"
     CDS             1641..3062
                     /locus_tag="VFF05_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZW51358.1"
                     /translation="MTDSVQVSGYKLVQKIGEGGMATVYLAMQESLDREVALKIMAPT
                     LAANETFREQFLKEGRIAAKLTHPHLMTVHDIGSENGVFYLASEYLPAGTLRDRMQRL
                     NAAEVLEIVRDVASGLSYAHEMGFVHRDVKPGNIMFRSNGSAVLADFGIAKAIKSVSA
                     ATMAGNAIGTPDYMSPEQAQATPVDGRSDLYSLGAVLFECVAGHKPYEAGDAYAVALM
                     HVTEPVPKLPEKLAWLQPLIDKSMAKQPADRFATGDAFVAELDRLLAANPQMDAVMRE
                     SRSARRRAVSAAAASSAAKPGGGRKWIFVGAGVAAAVVIVALAWMAWRGKAATNVAPT
                     VATSTPMETAPVAPPPPQPPESPVADARLAQLDLPTLLSRGDDYFNYGQKNGGEKMSF
                     PPGDNAIDLFQEALKRDPGNAQATQGLRKIAAFYANGAQGALKNGLYTGADVMVESGL
                     RADPKNAALQQLKAQLAKAEGGG"
     gene            complement(3069..3770)
                     /locus_tag="VFF05_05840"
     CDS             complement(3069..3770)
                     /locus_tag="VFF05_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZW51359.1"
                     /translation="MIEFGHNTHVGLRREHNEDTYYADSELGLWLVADGMGGHEHGEV
                     ASALARDTLVQEITQGMPLAKAVQRADEEIIQHSSKKAEALPMGTTIAAMRLHENEYE
                     ICWVGDSRVYLWNGELKQLSRDHSYVQELIQQGAITHEQARTHPHRNVVTQALGVTDP
                     ESLRVETIRGSLQPGMQVLLCSDGLTEEVDDKDIETVLARQDLSAQECVDHLILAALE
                     GGGSDNVTVILIRNR"
BASE COUNT          698 a         1244 c         1314 g          655 t
ORIGIN      
        1 cgacaatcgg catcgcgaac tgcacggcgc cttgctcgac gcgcaactgc tcgcggacgt
       61 gtacctggcg atgaccggag ggcagggcga tctcggcctt gccagcgcgc aggcggtcgc
      121 gccggcgcgg gttgcgacgc agttcgtcgc gcgtcggccg ttgcgcgtgc gtcgtgcgga
      181 tgccaccgaa gcagcgttgc acgcggcgcg cttgcaggcc atcgacaagg ccagcaaggg
      241 ccgctgcctg tggcgcgcct gagggcgtga cggtatgatc gggcggccag ttcggatcgt
      301 cctggggagg aaggcgatga atgcgatgac gacgttgctc gcgggaatgc tgctgtgcgc
      361 gttcggcgcg catgccgaaa cggcggtcga ggcgatcgcc gcgcacaagc acatgggcgt
      421 ggccacgtgc agcagcagcg tctgccacgg cgcatcgcaa ccgtttcgcg attcggatat
      481 cgcgcagaac gaattcgcgg tctggcagga attcgatccg cacgccaagg cgtaccaggt
      541 tctggaacag ccgcgttcgc aggaaatcgc gcgcaagctc ggcctcggcg atgccaccaa
      601 ggcgaaagtc tgtctggact gccacgccga caacatgccc gcgggcgacc gcggcgaacg
      661 cttccagatt tccgacggcg tgggctgcga ggcttgtcac ggcggcgcgg aactgtggct
      721 caattcgcac gccgaccgca aagtcaccta cgcggacgat ctggccaagg gcatgtatcc
      781 cacggccgat ccgatcaagc gcgcgcagtt gtgcctgtcc tgccacatgg gcgagaagga
      841 gcgcatgatc acgcaccgga tcatgggcgc cggccatccg cgcctgtcgt tcgagttgga
      901 taccttcacc tggctcaatc cgcactacaa gatcacggcg aactggatca agcgcaaagg
      961 cgaatggaat ggcgtgcgcg actgggccgt tggccagggc gtggccgcgg aaaacctgct
     1021 cggactgctg ctcgacccga aaaccggttg gcaggggata ttcccggaac tggtgctgtt
     1081 caactgccag gcctgccaca agctgatgaa gggcaacaat tgggggccgc gccagggcac
     1141 cggcctgggc ccgggcgtgg tgcgtctgaa cgatgcgaac ctggtgacgt ttcgctacgc
     1201 gctggcgccg gtcgacaaga ctgcggcgca gcgtgtgttc gagcaaaccc gtgcgctgca
     1261 tcaggcaacc acgcaaagcc gcgatgccac ctttgcggcg gcgcgtacgc tgaacgccac
     1321 gctggaaagc ctgctggcca aggtggccgc ctacaactac gacgcggcca gcctgaatcc
     1381 gatcctcgcg gcgatgcttg ccgatgcgca gcagggcgag ttccgcgatt atgccgcggc
     1441 cgaacaggca tcgatggcgg cgcaatcgct ggtcgtcgcg ttcgagaacg acggcaagct
     1501 ggacaaggcg catgccgcgg cgctgcacac caagctcgat ggcctgtatg ctgcggtgaa
     1561 ggatgaagac cgttattcga tggcgccttt cgtggccgct ttgaaggatt tgcgcgccgc
     1621 ggcgccttga gtttcgagaa atgaccgaca gcgttcaggt atccggctac aaactggttc
     1681 agaaaatcgg cgaaggcggc atggccacgg tctatctggc catgcaggaa tcgctcgacc
     1741 gcgaagtggc gctgaagatc atggcgccaa cgctggcggc gaacgagacg tttcgcgagc
     1801 agttcctcaa ggaaggccgc atcgcggcca agctcaccca tccgcacctg atgacggtgc
     1861 acgacatcgg ctcggaaaac ggggtcttct atctcgccag cgaatacctg ccggccggca
     1921 ccctgcgcga ccgcatgcag cgcctgaatg ccgccgaggt gctggagatc gtgcgcgacg
     1981 tcgcctccgg actttcctac gcccacgaga tgggtttcgt gcaccgcgac gtgaagcccg
     2041 gcaacatcat gttccgcagc aacggctcgg cggtgctggc ggacttcggc atcgccaagg
     2101 cgatcaagtc ggtcagcgcg gcgaccatgg ccggcaacgc catcggcacg cccgattaca
     2161 tgagtccgga gcaggcgcag gcgacgccgg tggatgggcg ctcggacctg tacagcctgg
     2221 gcgcggtgtt gttcgaatgc gtggccggac acaagcctta cgaggccggc gatgcctatg
     2281 cggtcgcgct gatgcacgtc accgagccgg tgccgaagct gccggaaaaa ctcgcctggt
     2341 tgcagccgct gatcgacaag tcgatggcca agcaaccggc cgaccgcttc gccacgggcg
     2401 atgctttcgt tgccgaactc gatcgcctgc ttgcggccaa tccgcagatg gatgcggtca
     2461 tgcgcgaatc gcgcagcgcg cgccggcgcg cggtctcggc cgccgccgca tcctcggccg
     2521 cgaaaccggg gggcggcaga aaatggattt ttgtcggcgc tggcgttgcg gccgccgttg
     2581 tgatcgtggc gctggcgtgg atggcttggc gcggcaaggc tgccacgaac gtcgcgccga
     2641 cagtggcaac cagcacgccg atggaaacgg ccccggtcgc gccgccgccg ccacaaccac
     2701 ccgaaagccc ggtcgccgat gcacgcctgg cacaactgga tttgccgacg ctgctctccc
     2761 gcggcgacga ctatttcaac tatggccaga agaacggtgg cgagaaaatg agttttccgc
     2821 ccggcgacaa cgccatcgat ctgttccagg aagcgctcaa gcgtgacccc ggcaatgcgc
     2881 aggcgacaca aggcttgcgg aagatcgccg cattttatgc gaacggcgcg cagggagcgt
     2941 tgaaaaacgg gctgtatacc ggcgccgacg tcatggtcga atcgggcctg cgtgcggatc
     3001 cgaagaatgc cgccttgcag cagctgaagg cgcaattggc gaaagccgaa ggcggcggct
     3061 gaagattttc agcggttgcg gatcagaatg acggttacgt tgtcggaccc gccgccttcg
     3121 agcgcggcaa ggatcaggtg atcgacgcat tcctgggccg aaaggtcctg gcgtgccagc
     3181 acggtttcga tgtccttgtc gtcgacttct tcggtcaggc catccgagca cagcagcacc
     3241 tgcatgccgg gctgcaggct gccgcggatg gtttccacgc gcaggctttc cggatcggtg
     3301 acgccgagtg cctgggtgac gacgttgcgg tgcggatggg tgcgcgcctg ctcgtgcgtg
     3361 atcgcgccct gctggatcag ttcctgcacg taggaatggt cgcgcgataa ctgcttgagt
     3421 tcgccgttcc acaaatacac gcggctgtcg ccgacccagc aaatctcgta ttcgttctcg
     3481 tgcaggcgca tcgcggctat ggtcgtgccc atcggcagcg cttcggcctt cttgctcgaa
     3541 tgctggatga tctcttcgtc ggcgcgctgc acggccttgg ccagcggcat gccctgggtg
     3601 atctcctgca ccagcgtatc gcgcgccagg gcgctggcta cctcgccgtg ctcgtggccg
     3661 cccatgccgt cggcgaccag ccacaggccg agctccgagt ccgcataata ggtatcttcg
     3721 ttatgctcgc ggcgcagacc gacatgggtg ttgtgtccga actcaatcat gcgtagccgg
     3781 tatcctgcgc agtccccatg gtccccgccg ccacggcgca acggcactca tagcatgccg
     3841 tgtcgccggg cacgcgcgca agcggcagcg cggcactctg ccggcacccg gcgcgaacgg
     3901 cgcgtgcggc c
//
LOCUS       DASRVO010000469         7136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_325529, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000469 DASRVO010000000
VERSION     DASRVO010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325529"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..1402)
                     /gene="rlmM"
                     /locus_tag="VFF05_05845"
     CDS             complement(350..1402)
                     /gene="rlmM"
                     /locus_tag="VFF05_05845"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011260144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HZW51360.1"
                     /translation="MSAALLLYCRAGFEGECAQEIEAGAHAAGIAGKARFDGGTGFVV
                     FAADATESALRDAVHWRDQVFARQSLIAFAHLPNLARNDRATPILAALRERGEKFHEV
                     WTEAPDSDAGKQLAPLCKSFGNALVGALKKAQLLDPQARRRLHVFFPQGDEVYLCAAD
                     PDAVAPWPQGIPRLKFPREAPSRSTLKLEEAFAVLLDEGERARWLKPGMTAVDLGAAP
                     GGWTWQLVRRSIRVTAVDNGPMDRALLDSGLVAHRREDGFRYRPPKPVDWLVCDMVEQ
                     PRRVADLVTHWLEQGWSRHAIFNLKLPMKKRFDETMRCLAQIRAGARLVRAKQLYHDR
                     EEITVFAASAGPRQKP"
     gene            complement(1399..2052)
                     /locus_tag="VFF05_05850"
     CDS             complement(1399..2052)
                     /locus_tag="VFF05_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZW51361.1"
                     /translation="MNAPAIRVVIADDHVLVRQGIRAFLDTHADLAIVAEAEDADGAV
                     SACTTHKPDVALIDLVMPGGGIAAIRAIHAASPATKSILLTSFDDARDILAAVQAGAL
                     SCLLKDIDADGLAEAIRKAARGEAVLHPRVAGYLVDAVRQGRSGPGVDALAALSERER
                     EVLALIAEGASNQRIATELEISEKTVKTHVGNMLAKLGLGDRTQAAVYAWKSGMKKP"
     gene            complement(2049..3491)
                     /locus_tag="VFF05_05855"
     CDS             complement(2049..3491)
                     /locus_tag="VFF05_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZW51362.1"
                     /translation="MKPLRPQLRGLIFKLTVFYVLLSLPCLVLVETGLLTFEFDRFMD
                     GVESGALTQAAQTAATDLARRWPNADTPANLELWTDALVLRMQRPHGGLLAQDSFILT
                     ELSSAPISATVLVEDGRALAAAPANTAWQPALPTPDSTAWRDTVAQAGKLARIADSPW
                     RVRRALAPIRTDDGHLYGYLLVELRLPVPWHRFLLDASLEWPIVLGYLILFGAASSFF
                     LATWVTRRLNRVAHAATAWSRGDFSERIDDTSRDELGRLSALLDDMALQLKDLLRSRA
                     QLAALAERQRLARDLHDTVKQQAFALNLQLATLRRHLGEHPESRRMEQAEALSQQIQR
                     ELAQLLDELRAGDTELPFVERLRTRAQTWSQTSGVKLDLQLDEVPAPSAGAAENLLRI
                     VDEALANVLRHSGATQVTVSLRRDENDAQLVVADNGHGMDSTLRSGMGLANMRERASA
                     LPRGRFEFDSAAAQGTRIRIVFSTAEDHPA"
     gene            complement(3555..5753)
                     /locus_tag="VFF05_05860"
     CDS             complement(3555..5753)
                     /locus_tag="VFF05_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZW51363.1"
                     /translation="MNHSTRRQLALALAVCSALTTTCAFAATGDDQSSSTQELKKVEV
                     KGYVMPDDAKPKLDHIMKEVDGPKITVTKKTSITKLDNLPTIIGNNPREVFAQTPGLY
                     IAEQQIPGQVNLSYRGIGNPQESEYVGVFQDGIPLEGDWIGFPTLYVFPLPQTIAEVQ
                     MIRGGSSLLYGPEPPPAINLVSAKPSTEPMAGYTENVIGNNGLFATFNKVSGTQGNWD
                     YLADAHYRTTDGDRDNGDAKLRGADLHIGYRPDETSYTALDFHAYDLDTGSPGALTFP
                     QYQANPDLTTTPYDRLWTQRYVLSLSHDRHLDENTELVAKVWAGYQDQASRSQDRGAA
                     PTTATLQDEQFRFTGIDARLVHHWGRGNAFTVGTTLYHADSPFRQWDDFGLASDEYDR
                     PGVPCTTPASTDCAKLRHARSTSYASVFAENVFRLPHQWHIVPSVRLDHEHVDIDETI
                     KPPNLNRGLVDRSVSHNVPLFGLGIGNDFGHLNESYFNVSQGWRPVRYFDVGSPFGST
                     NSGPLNDPRPPHVLNYELGVHGTPLHGLFYDVSVFQVNVKDRIESQPIANSPLGNTIN
                     VNTGNTRSRGFEGEVSYDFLQARDLHTTQHLAVFANLTLLDAEFTSTINPANLGNTPA
                     FSPKYLARAGITYREDKHLKIALSAVSVASQYYADANIVAPAPNLTPAKVPAYAVADF
                     SADYWVLPQLRLLAGVSNLTDKRYYSRVTLSSGLDPAPGRTVYAGFAYEF"
     gene            complement(5835..>7136)
                     /locus_tag="VFF05_05865"
     CDS             complement(5835..>7136)
                     /locus_tag="VFF05_05865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZW51364.1"
                     /translation="GDVLESLPGIEDRDTRFLAFDAAAYVYSRGGEYDLALSYVNQAL
                     AMGPDGRSMCAVRETQIWILQEGGKLQPDDALIRQGLDACRQVGATANANSILLQWAK
                     AFLDQDRPADALKLLEKNQAEVLATHSSALTSDFRSTMAQAYWRRGDLSHAREAAQNA
                     VELANQQDKAKSVADAYGVLYQVAKQQGDAAAALAWHEKYATADKAYLNDVSARALAY
                     QMVHQRVLDKQRQLVAATNRNAVLTLQQQVEAQKARTRLLYILLLLSGLLIVGGWAYR
                     TKRSQMKFQKLARRDGLTGIFNRQHFLESAQDALRECAHHTREACLMVMDLDHFKSVN
                     DMHGHAAGDDALKRTVATCQVLLRPNDVFGRLGGEEFAILVPDCPADVARRRAEAMRE
                     SIVAARREGDAAADDAMYTAKRAGRNRVEVHRATDEPATAT"
BASE COUNT         1167 a         2185 c         2464 g         1320 t
ORIGIN      
        1 gtaggtagat ccggagtttc caccgccgcc ataaacctgg ctgatgacga cttgcgcctg
       61 cacactaccc gcaacggcca tcaaggccag actcagcaat cgacaaacga attttccaga
      121 catgacattc cccgcaacga attgatcggt aaaagacgca tgctaggccg cctgtttgac
      181 actttcgtgt cgcacgcgac cacaccggcg gattgcgccg agctgcgcct actggtgaac
      241 cgccccccca gacgatcaca tcggatcgtt tgcgcggcgc cgtgccgcgt gccatggacc
      301 ggttgcgcgg cactctacaa cgaatttcgc ggcggtgcca cgaaccgggt caaggcttct
      361 gccgcggccc cgcggaagcc gcgaaaaccg tgatttcctc gcgatcgtgg tagagctgct
      421 ttgcgcgcac cagacgcgcg cctgcgcgga tttgcgcaag gcagcgcatc gtttcgtcga
      481 agcgcttttt catcggcagc ttgagattga agatcgcgtg acgggaccag ccctgttcca
      541 gccaatgcgt gacgagatcg gccacgcggc gcggctgctc gaccatgtcg cagaccagcc
      601 agtccaccgg cttcggcgga cgatagcgga acccgtcctc gcgccggtgc gcgacgaggc
      661 cggaatcgag cagcgcgcga tccatcggcc cgttgtcgac cgcggtgacg cggatcgaac
      721 ggcgcacgag ctgccaggtc cagccgcccg gcgcggcacc gagatccacg gccgtcatgc
      781 caggcttgag ccagcgcgcg cgttcgccct cgtcgagcag caccgcgaac gcttcttcga
      841 gtttcaacgt ggagcggctt ggcgcttcac gcgggaattt cagacgcgga atgccctgcg
      901 gccacggcgc gacggcgtcc ggatcggctg cgcacaaata tacttcgtcg ccttgtggaa
      961 aaaatacatg cagccgccgt cgtgcctgtg ggtcgagcag ttgcgccttc ttcaacgcgc
     1021 cgacgagggc gttgccgaag ctcttgcaca gtggcgccag ttgcttgccg gcgtcgctgt
     1081 ccggcgcttc ggtccagacc tcgtggaatt tctcgccgcg ttcgcgtaac gcggcaagaa
     1141 tcggcgttgc gcgatcgttg cgcgcaaggt tcggcaaatg cgcgaaagcg atcagcgatt
     1201 gacgcgcgaa gacctggtcg cgccagtgca cggcgtcacg cagcgccgat tcggtggcgt
     1261 ctgcggcgaa cacgacgaac cctgtgccgc cgtcgaatcg cgccttgccg gcgatgccag
     1321 ccgcgtgcgc gccggcttcg atttcctgcg cgcattcgcc ctcgaatccc gcgcggcaat
     1381 acagcagcag cgcggcgctc acggtttctt catgccgctt ttccaggcat acaccgctgc
     1441 ctgggtgcgg tcgccgaggc cgagcttggc gagcatgttg ccgacgtgcg tcttcacggt
     1501 cttttcggaa atttctagct cggtggcgat gcgctggttg ctggcgcctt cggcgatcag
     1561 tgccagcact tcgcgctcgc gttcgctcag cgcagccagc gcgtccacgc cgggaccgga
     1621 tctgccctgg cgcaccgcgt cgacgaggta acccgccacg cgcggatgca gcaccgcttc
     1681 gccgcgcgcc gccttgcgga tcgcttcggc gaggccatcg gcatcgatgt ccttgagcag
     1741 gcacgacagc gcgccggcct gcaccgcggc gaggatgtcg cgggcgtcat cgaacgaggt
     1801 gagcaggatc gactttgttg ccggactcgc ggcatggatc gcgcggatcg cggcgatgcc
     1861 gccgccgggc atgaccaggt cgatcagggc gacgtcgggt ttgtgcgtcg tgcaggcgga
     1921 tacggcgccg tcggcatcct cggcctcggc aacgatggca aggtcggcat gcgtgtcgag
     1981 aaaggcgcga atgccctggc gcacgagcac atggtcgtcg gcgatcacga cgcggatggc
     2041 gggtgcgttc atgcaggatg atcctcggcg gtggaaaaca cgatgcggat gcgcgtgcct
     2101 tgcgcggcgg cactgtcgaa ttcgaagcgg cctcgcggca acgcgcttgc acgttcgcgc
     2161 atgttcgcaa ggcccatgcc ggagcgcaat gtcgagtcca tgccgtggcc gttgtcggcg
     2221 acgacgagtt gcgcgtcgtt ctcgtcgcgg cgcaggctca ccgtcacctg cgtggcgccg
     2281 ctgtggcgca gcacgttcgc caatgcttcg tcgacgatgc gcaacaggtt ttccgcggcg
     2341 ccggcggacg gtgcgggaac ctcgtcgagt tgcaaatcaa gcttcacgcc gctggtctgc
     2401 gaccacgttt gcgcgcgcgt gcgcaggcgt tcgacgaaag gcagttcggt gtcgccggca
     2461 cgcagttcgt cgagcagctg cgcgagttcg cgctggatct gctggctcag cgcttcggcc
     2521 tgttccatgc gccggctttc cgggtgttcg ccgagatggc ggcgcaaggt cgccagttgc
     2581 agattcagcg cgaacgcctg ctgcttcacg gtgtcgtgca ggtcgcgcgc caggcgctgg
     2641 cgttcggcga gtgcggcgag ctgcgcgcgc gaacgcagca ggtccttgag ctgcagtgcc
     2701 atgtcgtcga gcagggcgga aaggcgcccg agttcgtcgc gcgaggtgtc gtcgatgcgt
     2761 tcggaaaaat cgccgcgact ccatgcggtg gcggcgtgcg ccacgcggtt gagccggcgc
     2821 gtgacccagg tggcgaggaa gaacgacgag gccgcgccga acaggatcag gtaaccgagc
     2881 acaatcggcc attccaggct ggcatcgagc aggaagcggt gccacggcac cggcaggcgc
     2941 aattccacga gaagatagcc gtaaagatgt ccatcgtcgg ttcggatcgg cgccagcgcg
     3001 cggcgcacgc gccacgggct gtcggcgatg cgtgccagct ttcccgcctg cgctaccgtg
     3061 tcgcgccagg cggtcgaatc cggcgtcggc aacgccggct gccatgccgt gttcgccggc
     3121 gcagcagcga gcgcacgtcc atcctcgacg agcaccgtgg cggaaatcgg cgcggaagaa
     3181 agctcggtga ggatgaagga atcctgcgcc agcaggccgc cgtgcgggcg ctgcatgcgc
     3241 agtaccagcg catcggtcca caattccagg ttcgcgggcg tatccgcgtt cggccagcgt
     3301 cgcgcgaggt ccgtggcggc ggtttgtgcg gcctgggtca gcgcgccgga ttccacgccg
     3361 tccatgaagc ggtcgaactc gaacgtgagc agtcccgtct ccaccagcac caggcacggc
     3421 agcgagagca gcacgtagaa gacggtgagc ttgaagatca gtccgcgcag ttgcggacgc
     3481 agcggtttca tgcgcgggac gaagcaaggg ccggatgcca agcatagcgg catccggcct
     3541 ttggcggcgt tgcgtcagaa ctcgtaagcg aatccggcgt agaccgtacg tcccggcgcc
     3601 ggatcaaggc cgctggacag cgtcacgcgc gagtagtagc gcttgtcggt caggttggaa
     3661 acgccggcga gcaggcgcag ttgcggcagt acccaatagt cggcggagaa atcggccacc
     3721 gcgtacgcgg gcaccttggc cggcgtgagg ttgggtgccg gcgcgacgat gttcgcgtcc
     3781 gcgtagtact ggctggcgac cgaaaccgcc gacagcgcga ttttcaggtg cttgtcttcg
     3841 cggtaggtga tgccggcgcg ggccaagtac ttgggcgaga acgccggcgt gttgccgagg
     3901 ttggccgggt tgatcgtcga ggtgaattcc gcgtcgagca gggtaaggtt ggcgaacacg
     3961 gccagatgct gcgtcgtgtg caggtcgcgc gcctgcagga aatcgtagga cacctcgcct
     4021 tcgaagccgc ggctgcgcgt attgccggta ttcacgttga tcgtgttgcc cagcggcgaa
     4081 ttcgcgatcg gttgcgattc gatgcgatcc ttcacgttca cctggaacac gctcacgtca
     4141 tagaacaaac cgtgcagcgg cgtgccatgc acgccgagtt cgtaattgag cacatgcggc
     4201 ggtcgcgggt cgttcagcgg accggaattg gtgctgccga aaggcgagcc gacgtcgaaa
     4261 tagcgcaccg ggcgccagcc ctgcgagacg ttgaaataac tctcgttgag atggccgaaa
     4321 tcgttgccga tgccaaggcc gaacagcggt acgttgtggc tcaccgaacg gtcgacgagc
     4381 ccgcggttga gattgggcgg cttgatcgtc tcgtcgatgt ccacgtgttc gtgatccagg
     4441 cgcaccgacg gcacgatgtg ccattggtgc ggcaggcgaa acacgttctc ggcgaaaacg
     4501 gatgcatagc tggtcgagcg tgcgtggcgc agcttggcgc agtcggttga cgcgggcgtg
     4561 gtgcagggaa cgccggggcg gtcgtattca tccgaagcaa gcccgaaatc gtcccattgc
     4621 cggaacggcg agtccgcgtg atacagcgtg gtgccgacgg taaacgcgtt gccgcggccc
     4681 cagtgatgta ccaggcgcgc atcgattccg gtgaagcgga actgctcgtc ctgcagtgtc
     4741 gcggtggttg gtgccgcgcc acgatcctgc gaacgcgagg cctggtcctg atagccggcc
     4801 cagaccttgg cgacgagttc ggtgttttcg tcgaggtggc ggtcgtggct caggctcaac
     4861 acatagcgct gtgtccacag ccggtcgtag ggcgtggtcg tcaggtccgg gtttgcctgg
     4921 tattgcggaa aagtcagggc gccggggctg cccgtatcca ggtcgtaggc gtggaaatcc
     4981 agcgccgtgt acgaagtttc gtcggggcgg tagccgatgt gcagatccgc gccgcgcaat
     5041 tttgcatcgc cgttgtcgcg atccccgtcc gtcgtgcggt aatgcgcatc ggcgaggtaa
     5101 tcccagttcc cctgcgtgcc tgagaccttg ttgaaggtgg cgaacaggcc attgttgcca
     5161 atgacattct cggtgtagcc ggccatcggc tccgtcgacg gtttggcgct gaccagattg
     5221 atcgccggtg gcggttccgg tccgtacagc agcgaggagc cgccgcggat catctgcact
     5281 tcggcgatgg tctgtggcag cgggaacaca tacagcgtgg ggaaaccgat ccagtcgcct
     5341 tccagcggga tgccgtcctg gaacacgccc acgtattcgg attcctgcgg attgccgatg
     5401 ccgcgatacg acagattcac ctgtccggga atctgctgct cggcgatgta caggcccggc
     5461 gtctgcgcaa agacttcgcg cggattgttg ccgatgatgg tgggcaggtt atcgagcttg
     5521 gtgatggagg ttttcttggt gaccgtgatc ttcggcccgt cgacttcctt catgatgtgg
     5581 tcgagcttgg gcttggcgtc gtcgggcatg acgtagccct tgacctcgac tttcttcagt
     5641 tcctgcgtcg aagaggattg atcgtcgccg gtggcggcga atgcgcaggt ggtggtcagg
     5701 gcggagcaga ctgccagcgc cagggccaat tggcggcgag tggaatggtt cacgtgggtt
     5761 ccccaatgag tcgatgcagc cggcgattgt ccgtgtttgc gcgtgccggc gcgtagtgcc
     5821 tgggggacta ggtctcaggt cgcagtggcg ggctcgtccg tggcgcggtg gacttcgacg
     5881 cggttgcggc cggcgcgctt ggcggtgtac atggcatcgt cggcggcggc atcgccttcg
     5941 cgccgtgcgg caacgatgga ttcgcgcatc gcttcggcgc gccggcgggc gacgtcggca
     6001 ggacagtcgg gtaccaggat ggcgaactcc tcgccgccaa ggcgtccgaa gacgtcgttc
     6061 gggcgcagca gcacctggca tgtcgcgacc gtgcgcttga gcgcgtcgtc accggcagcg
     6121 tggccgtgca tgtcgttgac cgacttgaag tgatccaggt ccatcaccat caggcaggcc
     6181 tcgcgcgtgt ggtgagcgca ttcgcgcaga gcgtcctgcg cggattcgag gaaatgctgg
     6241 cggttgaaga tgccggtcag gccatcgcgc cgtgccagct tctggaactt catctgcgag
     6301 cgcttggtgc ggtatgccca gcctccgacg atgagcaggc cgctgagcag gagcaggatg
     6361 tagagcaggc gtgtgcgcgc cttctgcgct tcgacctgtt gctgcagggt cagcactgcg
     6421 tttcggttag tggccgccac gagctggcgt tgcttgtcga gcacgcgctg gtgcaccatc
     6481 tggtaggcca gagcgcgcgc gctgacgtcg ttgaggtaag ccttgtcggc tgtcgcatat
     6541 ttttcgtgcc aggccagcgc ggcggcagcg tcgccttgct gcttggcgac ctggtagaga
     6601 acgccgtagg cgtcggccac ggacttggcc ttgtcctgct ggttcgccag ctcgaccgcg
     6661 ttttgcgcgg cctcgcgcgc gtgggaaagg tcgcccctgc gccaataggc ttgcgccatg
     6721 gtcgaacgaa agtccgaggt caatgcggag gaatgggttg ccaggacttc cgcctggttc
     6781 ttctcgagca gtttcagggc gtccgcaggc cggtcttgat ccaggaaagc cttggcccat
     6841 tgcaagagta tggaattggc attggccgtg gcaccgacct ggcgacaggc gtccaggccc
     6901 tgccgaatca gcgcgtcgtc gggttgcagc ttgccgcctt cctgcaatat ccagatctgg
     6961 gtttcccgga ctgcgcacat cgagcgcccg tcgggcccca tggcgagcgc ctgattgacg
     7021 taactcagcg ccagatcgta ttcgccgccg cggctgtaga cgtacgcggc ggcatcgaag
     7081 gcaagaaaac gcgtatcgcg atcttcgatt ccgggcagcg attcgagcac gtcgcc
//
LOCUS       DASRVO010000470         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_13652, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000470 DASRVO010000000
VERSION     DASRVO010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13652"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /gene="atpH"
                     /locus_tag="VFF05_05870"
     CDS             <1..362
                     /gene="atpH"
                     /locus_tag="VFF05_05870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HZW51365.1"
                     /translation="DTIVARQGVSREVRNLMAVLIDHRRIAALTEITRQIELEIDRRL
                     GLAEAAVTSARELSARERLDLESQIERLTGKKIRAHYALDRTLLGGAMVRLGSTIYDG
                     TVRGQLQRLKGQLSESS"
     gene            459..>1064
                     /locus_tag="VFF05_05875"
     CDS             459..>1064
                     /locus_tag="VFF05_05875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZW51366.1"
                     /translation="MAIKADEVTKLIREQIENYESRIAVDEVGTVISLGDGIARVHGL
                     DKVMAGELLSLPHGVAGIAMNLEEDQVGVVLLGEYTEIKEGDEVKRTGRIASVPVGEA
                     LIGRVVNALGQPIDDKGPINTTQFLPVERIAPGVIDRQPVREPMATGLKAIDAMIPIG
                     RGQRELIIGDRGTGKTAIAVDTIINQRDRGVICVYAAVGQKA"
BASE COUNT          220 a          328 c          331 g          185 t
ORIGIN      
        1 tcgacacgat cgtggccagg cagggcgtct cgcgcgaagt gcgcaacctg atggccgtgc
       61 tgatcgatca tcgccgcatc gccgcgctta cggaaatcac gcgccagatc gaactcgaaa
      121 tcgaccgccg cctgggatta gcggaagccg ccgtcaccag cgcgcgcgag ttgtcggcgc
      181 gggaacggct cgacttggaa tcgcaaatcg agcgccttac cggaaaaaag atccgcgcgc
      241 attatgccct cgaccgcacg ctgctgggcg gcgccatggt ccggctcggc agcaccattt
      301 acgacggcac cgtgcgcggg cagttgcagc gtttgaaggg acagttgtcg gaaagcagtt
      361 gatcgttgct ggttgttagt tgctggtgat tgcgttctca gcgctgctgg cgatcaacaa
      421 ctgacaactg gcaactagca actaagaact ggttttctat ggctatcaaa gcagacgaag
      481 ttacgaaact gattcgcgag cagatcgaga attacgaatc tcgcatcgcg gtagatgaag
      541 tcggcaccgt catcagcctc ggcgacggta ttgcccgcgt tcatggcctc gataaggtca
      601 tggcgggcga actgctctcg cttcctcacg gcgtcgccgg catcgccatg aacctggaag
      661 aagaccaggt cggcgtcgtg ctgctcggcg aatacacgga gatcaaagag ggcgacgagg
      721 tcaagcgcac cggccgtatc gccagcgtgc ccgtgggcga ggcgctcatc gggcgcgtcg
      781 tgaacgcact cggccagccc atcgacgaca aaggtccgat caacacgacg cagtttcttc
      841 cggtcgagcg catcgctccc ggcgttatcg atcgccagcc cgtgcgcgag ccgatggcta
      901 ccggcctgaa ggcgatcgat gccatgatcc cgatcggccg cggccagcgc gaactgatca
      961 tcggcgaccg cggcaccggc aagaccgcga tcgccgttga taccatcatc aaccagcgcg
     1021 accgcggcgt gatctgcgtc tatgccgcgg tcggccagaa agca
//
LOCUS       DASRVO010000471         1334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_65653, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000471 DASRVO010000000
VERSION     DASRVO010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65653"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFF05_05880"
     CDS             complement(<1..921)
                     /locus_tag="VFF05_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZW51367.1"
                     /translation="MDLAATSGLLRLLADPTRLRLLALIEREELTVAELAGVLRLAQP
                     RVSTHLAKLKEAGLVRDRRAGVSAYYRWNAEADGKTAQLVQALRASLDDTLIADDLRR
                     LFSMLAQRARAAGWADSVAGDMERHYSPGRTWEALARALTSLIDVGDVLDVASGDGVL
                     AELLAPHAKSILCIDASERVVAAARARLKSFRNVHVRLGNMHALDLGARRFDLVTLMH
                     ALTYSEDPARAVAEAARVLKPGGRLSAITLGRHAHRAAVAPFDHRNLGFRAEDLRRFA
                     QSAGLKIIACGTITRERRPPHFEVLHLVARK"
     gene            948..>1334
                     /locus_tag="VFF05_05885"
     CDS             948..>1334
                     /locus_tag="VFF05_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51368.1"
                     /translation="MDAQITLAFRQRQRSGLNRLDPLWSNARAWRWPVRTRRRWWTWI
                     AAWLSLAAAPVGAEVPYAALAKFWTPDALHVPYRAMQTPGRLDGEKLPLIVFLHGDWQ
                     DGTNNESQLAGYGNGSMLWLDQANHGD"
BASE COUNT          229 a          412 c          475 g          218 t
ORIGIN      
        1 ttttctcgcg accaggtgca acacttcgaa atgcggcgga cggcgttcgc gcgtgattgt
       61 tccgcaggcg atgattttca atccggcgga ctgcgcgaag cggcgcaagt cctcggcgcg
      121 gaagccgaga ttgcggtgat cgaatggcgc cactgcggca cgatgcgcat ggcggccgag
      181 cgtgatcgcg gacaggcgcc cgccgggctt gagcacgcgt gcagcctcgg cgacggcgcg
      241 cgccgggtct tcgctatagg tcagcgcgtg catgagcgtg accaggtcga agcggcgcgc
      301 gccgagatcg agcgcgtgca tgttgccgag gcgaacgtgc acgttgcgga aggatttcag
      361 gcgcgcgcgc gctgcggcga ccacgcgctc gctcgcgtcg atgcagagga tggatttggc
      421 atgtggcgcg agcagttccg ccagcacgcc gtcgcccgaa gccacgtcga gcacgtcgcc
      481 gacgtcgatc aggctggtca gcgcacgcgc aagcgcttcc caggtccggc ccggcgagta
      541 gtgtcgctcc atgtcgccgg ccacggaatc cgcccagccg gcggcacgcg cgcgctgcgc
      601 cagcatcgaa aacaggcggc gcaggtcgtc ggcgatcagc gtgtcgtcga gactggcgcg
      661 cagggcctgc acgagttgcg cagtcttgcc gtcggcttcc gcgttccagc ggtaatacgc
      721 cgacacgcca gcgcgacggt cgcgcacgag ccctgcttct ttcaatttgg ccagatgcgt
      781 ggacacgcgc ggctgcgcca gacgcagcac gccggcgagc tcggcgacgg tcagctcctc
      841 gcgctcgatc agggcgagca ggcgcaggcg cgtggggtcg gcgagcaggc gcagcaggcc
      901 ggaagtggca gcgagatcca agtgtatcct ttcatcttga ttgaaagatg gatgcgcaga
      961 ttacgctggc gttccgccag cgtcaacgat ctggattgaa tcggctcgat ccgttatggt
     1021 cgaacgcgcg ggcgtggaga tggccggtgc gcacaagacg acggtggtgg acatggattg
     1081 cggcgtggct gagtctcgcc gccgcgcccg taggcgcgga agtgccgtat gcggcattgg
     1141 ccaaattctg gacaccggat gcactgcacg tgccttatcg cgccatgcag acgcccggac
     1201 gactcgacgg cgagaaactt ccgttgatcg tgttcctgca cggcgactgg caggacggca
     1261 ccaacaacga atcgcaactg gccggctacg gtaacggctc gatgttgtgg ctggatcagg
     1321 cgaatcatgg cgat
//
LOCUS       DASRVO010000472         1486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_65679, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000472 DASRVO010000000
VERSION     DASRVO010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65679"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="serC"
                     /locus_tag="VFF05_05890"
     CDS             <1..663
                     /gene="serC"
                     /locus_tag="VFF05_05890"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZW51369.1"
                     /translation="DAAYLHYTPNETIHGVEFHDVPDAGGLPLVADMSSNILSRPVDV
                     SKFGVIYAGAQKNIGASGLVVMIVREDLLARCPKDIPKIFNYAEHAANESMLNTPNTF
                     GWYLAGLVFEWLLEQGGVAAMGDRNRAKAELLYGYIDTSGFYRNPVEKSARSWMNVPF
                     TLPREELDVAFLKEAEAAGLMALKGHRAVGGMRASLYNAMPLEGVQALVAFMKDFAAR
                     NA"
     gene            663..>1486
                     /locus_tag="VFF05_05895"
     CDS             663..>1486
                     /locus_tag="VFF05_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HZW51370.1"
                     /translation="MFRIQTLNNISRNGLDRLPANLYSVANDIAEPDALLVRSADLHG
                     KPLAASIKAVARAGAGTNNIPVTEFSQRGVPVFNAPGANANAVKELVIAGMLIAARNL
                     DSALAFVGGLDGGAHLHDKVEAEKKRFVGSELAGKTLGVVGLGAIGVKVANAARALGM
                     RVLGFDPHMTVDGAWALSSDVTKAGSLNELYAASDYITFHVPLNEATRGVFGAAALEL
                     VRLGTVVLNFAREGIVDAAALTQGLASGRIGRYVTDFPSADLVGNARVIGLPHLGAS"
BASE COUNT          257 a          486 c          490 g          253 t
ORIGIN      
        1 gacgcggcct acctgcacta cacgccgaac gaaaccatcc acggcgtcga attccacgac
       61 gttcccgacg ccggcggcct gccgctggtc gcggacatgt cctcgaacat cctgtcgcga
      121 ccggtcgacg tgtcgaaatt cggcgtcatt tacgccggtg cccagaagaa catcggcgcc
      181 tcgggcctgg tcgtgatgat cgtgcgcgaa gacctgctcg cgcggtgccc gaaggacatc
      241 ccgaagatct tcaactatgc cgaacacgcg gccaacgaat cgatgctcaa cacgccgaac
      301 acgtttggct ggtatctggc cgggctcgtg ttcgaatggc tgctggagca gggcggcgtg
      361 gcggcgatgg gcgatcgcaa ccgtgccaag gccgagctgc tttatggcta tatcgacacc
      421 tcgggtttct acaggaatcc ggtggaaaaa tccgcgcgtt cgtggatgaa cgtgccgttc
      481 acgctgccgc gcgaggaact cgacgtggca ttcctgaagg aagccgaggc tgcgggcctg
      541 atggcgctca agggccatcg cgcggtcggc ggaatgcgcg cctcgctgta caacgccatg
      601 ccgctggaag gcgtgcaggc gctggtggct ttcatgaaag acttcgcggc gaggaacgcg
      661 tgatgttcag gatccagacc ctcaacaaca tctcgcgcaa cggactcgat cggctgccgg
      721 ccaatctgta ttcggtcgcg aacgacatcg ccgaacccga tgcgctgctg gtgcgctcgg
      781 cggacctgca cggcaagccg ctggccgcat cgatcaaggc cgtcgcgcgt gccggcgcgg
      841 gcacgaacaa cattcccgtg accgagttca gccagcgcgg cgtgccggtg ttcaacgccc
      901 cgggcgcgaa cgcgaatgcg gtcaaggaac tcgtcatcgc cggcatgctg atcgccgcgc
      961 gcaacctcga cagcgcgctc gcctttgtcg gcggactgga cggcggcgct catctgcacg
     1021 acaaggtcga agcggagaag aagcgcttcg tcggttcgga gctggccggc aagacgctcg
     1081 gcgtcgtcgg cctcggcgcg atcggcgtca aggtcgccaa cgcggcgcgc gcgctcggca
     1141 tgcgcgtgct cggcttcgat ccgcacatga ccgtcgacgg tgcctgggcc ctgtcctcgg
     1201 acgtcaccaa ggccggatcg ctgaacgaac tgtatgccgc ttcggactac atcacgttcc
     1261 atgtgccgct caacgaggcg acgcgcggcg tgttcggcgc cgccgcgctc gaactcgtgc
     1321 gactcggcac tgtcgtgctc aacttcgcac gcgagggcat cgtcgatgcg gcggcattga
     1381 cgcaagggtt ggcgagcggc cgcatcggcc gttacgtcac cgattttccg agcgccgact
     1441 tggtcggcaa cgcgcgcgtg atcggtttgc cgcacctggg cgcctc
//
LOCUS       DASRVO010000473         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_168497, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000473 DASRVO010000000
VERSION     DASRVO010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168497"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFF05_05900"
     CDS             <1..342
                     /locus_tag="VFF05_05900"
                     /inference="COORDINATES: protein motif:HMM:NF022537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2878 family protein"
                     /protein_id="HZW51371.1"
                     /translation="CIRGGLFVYAAPVPWPDYAPIWIVALWMSFALTLNHSLAWLKSH
                     LLLASVLGAIGAPLAYLAAARGWNALAIAADPALAFGALALAWAILTPALFWLARRLT
                     SAPATSSLREA"
     gene            345..1133
                     /locus_tag="VFF05_05905"
     CDS             345..1133
                     /locus_tag="VFF05_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HZW51372.1"
                     /translation="MNPLNILYVWIVACVAMFIGWLIQRRTHNAGIVDAIWAAGMGAS
                     ALFYAAVGSGGLASRLAVALLGSLWGFRLCLHILARVLNESEDGRYQYLREYWHGDQR
                     KFFAFFMGQALLIALFSLPFAAVAENPRETFSVWCGVGLAIWIVSLAGESIADAQLAA
                     FRTNPQNRGKACRVGLWRYSRHPNYFFEWLHWFTYVFLAFGAPFPAWLLSLLGPVLMV
                     ASLVWITGIPFTEAQALRTRGDDYRDYQRTTSMFIPWFPKRSHS"
     gene            1130..2206
                     /locus_tag="VFF05_05910"
     CDS             1130..2206
                     /locus_tag="VFF05_05910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZW51373.1"
                     /translation="MTSGASETLSMAAPAESGLTGLCERGLLPDWLIRAGIRHLCAQR
                     LSEERSDDTAAAWARFQHCLDELRASPVAINTDAANAQHYELPPRFFESCLGKRLKYS
                     SCYFPRGDETLDQAEDAMLALYGERAQLVDGQAILELGCGWGSLTLWMAQNYPRSHIT
                     AVSNSKPQREFILGRCRTLGLGNVEVVTEDVNRLGLPTSSFDRCVSIEMFEHMRNYAT
                     LLERIASWLRADGKLFVHIFCHRELMYPFETQGRDNWMGRYFFTGGLMPAADTLLWFQ
                     DDLAIERQWRLPGTHYAKTANLWLANQDANRAAVLQVLGQAYGEQARVWHQRWRMFWM
                     ACAELFGYRDGNEWLVAHYLFAKR"
     gene            complement(2260..>2960)
                     /locus_tag="VFF05_05915"
     CDS             complement(2260..>2960)
                     /locus_tag="VFF05_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZW51374.1"
                     /translation="GQFARGGGWDPMVTGGFGMQYNMSDRLAFRGEIAARYDRDNNSL
                     NSSLAASEGFHHHSGFTDGVVTVGLTYNFGGASAPAPAPTHEEAAAPPPAAPPAREEA
                     TPPAAVSIDLRGVEFKFDHPRVGEKLVPSLKAPTSESVQILDEAVDTLKRYPNVHVSV
                     DGYTDSVGSDAYNQKLSERRASGVYGYLTEHGIDASRLDGPKGFGEASPIDTNKTSAG
                     RQRNRRVELKVESQ"
BASE COUNT          492 a          940 c          936 g          592 t
ORIGIN      
        1 tgcattcgcg gcggcttgtt cgtatacgcg gcgcccgtgc cgtggcccga ctatgcgccg
       61 atctggatcg tcgccctgtg gatgagcttc gcgctgaccc tgaaccattc gctggcctgg
      121 ctgaaatcgc atctgctgct ggcttccgtg ctcggcgcca tcggtgcgcc gctcgcctac
      181 cttgccgccg cgcgcggctg gaacgcgctg gcaatcgccg ccgatcccgc gctcgcattc
      241 ggcgcgctgg cattggcatg ggcgatcctg acaccggccc tgttctggct ggccaggcgc
      301 ctgacgtcgg cgccggcgac gtcatcattg cgcgaggcct gacgatgaat ccgttgaata
      361 tcctgtatgt ctggatcgtg gcctgcgtcg ccatgttcat cggctggctg atccagcgcc
      421 gcacccacaa cgccggcatc gtggatgcga tctgggcagc aggcatgggc gcaagcgcct
      481 tgttctacgc agcggtcggc agcggcggtc tcgcgtcgcg gctcgcggtc gccctgctcg
      541 gcagcttgtg gggtttccgc ttgtgcctgc atatcctcgc gcgcgtactc aacgaaagcg
      601 aagatgggcg ctatcagtac ctgcgcgaat actggcacgg cgaccagcgc aagttcttcg
      661 cgttcttcat gggccaggcc ctgctgatcg cgctgttctc gctgccgttc gcggccgtgg
      721 ccgagaatcc gcgcgagaca ttctcggtct ggtgcggagt cggtctggcg atatggatcg
      781 tgagcctggc cggcgaatcc attgccgatg cgcaactggc cgcctttcgc acaaatccgc
      841 agaaccgcgg caaggcctgc cgtgtcggac tgtggcggta ttcgcgccat ccgaactatt
      901 tcttcgaatg gctgcactgg ttcacctatg tgttcctcgc cttcggcgca ccgttcccgg
      961 cctggctgct cagcctgctc ggaccggtcc tgatggtcgc atccctggta tggatcaccg
     1021 gcatcccgtt caccgaggcg caggcgctgc gcacgcgcgg cgacgattac cgcgattatc
     1081 agcgcacgac gtccatgttc attccatggt ttccgaaaag gtcgcattca tgacgagtgg
     1141 agctagcgaa acgctgagca tggccgcacc ggccgagagc ggcctgaccg gtctttgcga
     1201 acgcggcctg ctgccggact ggctcatccg tgcgggcatt cgccaccttt gcgcgcaacg
     1261 tctgagcgaa gaacgatcgg acgacacggc ggcggcatgg gcgcggttcc agcattgcct
     1321 cgacgagctg cgcgccagtc cggttgcgat caacaccgat gcagcgaacg cacaacatta
     1381 cgaattgccg ccgcgctttt tcgaatcctg ccttggcaaa cgcctgaaat attcgagctg
     1441 ctattttccg cgcggtgacg aaacgctgga tcaagccgag gacgcgatgc tcgcccttta
     1501 cggcgaacgt gcgcagcttg tcgacggaca ggccattctc gaactcggtt gcggttgggg
     1561 ttcgctgacg ctgtggatgg cgcagaatta tccgcgcagc cacatcaccg ccgtatccaa
     1621 ctcgaaaccg caacgcgaat tcatcctcgg ccgttgccgc acgctcggct tgggcaacgt
     1681 cgaggtcgtc accgaggacg tcaatcggct gggactcccg acgtcgagct tcgaccgctg
     1741 cgtatccatc gagatgttcg agcacatgcg caactatgcc accctgctcg aacgcatcgc
     1801 ttcgtggctg cgcgcggatg gcaaactttt cgtgcacatc ttctgccatc gcgagctgat
     1861 gtatccgttc gagacgcaag gccgcgacaa ctggatgggc cgctatttct tcaccggcgg
     1921 cctgatgccg gcggccgata ccctgctctg gttccaggac gatctcgcga tcgaacgcca
     1981 atggcgattg ccgggaacgc attatgcgaa gaccgccaat ttgtggcttg ccaatcagga
     2041 tgcgaaccgc gccgccgtgc tccaggttct ggggcaggcc tacggcgaac aggcacgtgt
     2101 ctggcaccag cgctggcgca tgttctggat ggcttgtgcg gagttgttcg gttatcgcga
     2161 cggcaacgag tggctggttg cgcactatct ctttgcgaag cgctgaaaag aaaaagcccg
     2221 gcattgccgg gctttttcgc attgcagtga gcggaaccct tactggcttt ccaccttcaa
     2281 ctcgacgcga cggttgcgct gacggccagc cgaggtcttg ttggtgtcga tcgggctggc
     2341 ttcgccgaag cccttcgggc catccagacg gctggcatcg atgccatgct cggtcaagta
     2401 gccgtacacg ccgctggcgc gacgctcgga cagcttctgg ttgtacgcat cgctgccgac
     2461 ggagtcggtg tagccgtcca ccgaaacgtg cacgttcgga taacgcttga gcgtatcgac
     2521 ggcctcatcg aggatctgca cggactcgga ggtcggggcc ttgagcgacg gcaccagctt
     2581 ctcgccgacg cgcgggtgat cgaacttgaa ttcgacgcca cgcaggtcga tggaaaccgc
     2641 ggccggcgga gtggcctctt cgcgggccgg cggagctgcc ggtggcggag ctgccgcctc
     2701 ttcatgcgtc ggcgccggag ccggagccga agcgccgccg aagttgtagg tcaggccgac
     2761 cgtgacgacg ccatcggtga aaccggagtg gtgatgaaaa ccttccgaag ccgccagtga
     2821 ggaattcagc gaattgttgt cgcggtcgta acgcgccgcg atttcgccgc ggaatgcgag
     2881 acggtcggac atgttgtact gcatgccgaa accaccggtg accatcgggt cccaaccgcc
     2941 gccacgggcg aactgacctt
//
LOCUS       DASRVO010000474         1062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_117451, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000474 DASRVO010000000
VERSION     DASRVO010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117451"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1062
                     /locus_tag="VFF05_05920"
     CDS             <1..>1062
                     /locus_tag="VFF05_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51375.1"
                     /translation="AYVNRGYVLNDLQNAEQATSDFQHALKLEPKNGEAHLGLAFAQL
                     ELHHGHAALQQAELAQKYMGESGSVYVAKATAYRQQGLLQQAEKAYRSALQYNRGDLQ
                     LTLALADTLFHMRRYDDSVKVLNAALALDSDDPFIYAQMAQAKAELKQRADTFKYVQA
                     AEREGADRSDVLLATGDALLTLGDRDAAMQRFERALDAPDSDRVQARLHIAKLMQRDG
                     NWDEAREQVALAFAESRIGEASPVTAENMLAAGDIFLRMHDFDLAQKLFQRARAAGAA
                     DQVVSIALANTYLAQGNTEQAENELASLGNPSDYQENYDYMLARANMLRQQHQDVRAM
                     TAFAHANMVSGEDTVAERQ"
BASE COUNT          210 a          352 c          356 g          144 t
ORIGIN      
        1 cgcgtacgtc aaccgcggct acgtgctgaa cgacctgcag aacgcggagc aggcgacctc
       61 cgacttccag cacgcgctga aactggagcc caagaatggc gaggcgcacc tgggtctggc
      121 cttcgctcaa ctcgaattgc atcacggtca cgccgcactg cagcaggccg aactggcgca
      181 aaagtacatg ggcgaaagcg gaagcgtgta cgtggccaag gcgaccgcct atcgccagca
      241 ggggctgctg caacaggcgg agaaggcgta ccgctcggcg ctgcagtaca accgcggcga
      301 cctgcaactc acgctggcgc tggccgacac cttgtttcac atgcgccgct acgacgattc
      361 ggtgaaggtg ctgaacgcgg cgctggcgct cgattccgac gaccctttca tctacgcgca
      421 gatggcgcaa gccaaggcag aactgaagca gcgcgccgac accttcaagt acgtgcaggc
      481 ggcggagcgc gagggcgccg accgctccga cgtgctgctg gccacgggcg acgcgctgct
      541 cacgctgggc gaccgcgacg ccgccatgca gcgcttcgag cgcgcgctcg atgcgccgga
      601 ttccgaccgc gtgcaggcgc ggctgcacat tgccaagctg atgcagcgcg acgggaactg
      661 ggacgaggcg cgcgaacagg tggcgctggc gtttgccgaa tcgcgcatcg gcgaggcttc
      721 gccggtcacc gcggagaaca tgctggcagc gggcgatatc ttcctgcgca tgcacgactt
      781 cgacctggcg caaaagctgt tccagcgcgc cagggccgcg ggcgccgccg accaggtggt
      841 gtccatcgcg ctggccaaca cttacctggc gcagggcaac acggaacagg cggagaacga
      901 actggcgagc ctgggcaacc cctccgatta ccaggagaat tacgactaca tgctggcgcg
      961 cgccaacatg ctgcgccaac agcaccagga cgtgcgcgcc atgacggcgt tcgcgcacgc
     1021 caacatggtg tcgggcgaag acacggtcgc cgagcggcag tt
//
LOCUS       DASRVO010000475         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_90982, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000475 DASRVO010000000
VERSION     DASRVO010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90982"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFF05_05925"
     CDS             <1..336
                     /locus_tag="VFF05_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HZW51376.1"
                     /translation="VRVLDPTTRESYAVTPRELAAFGKSADTRASLYRPSAQAAGRHG
                     GYRGRTGIYEYVPVDEELRRLIHANAAEADMERHARTRTPSILDDGWNKCIAGVTSAE
                     EVLRVTREE"
     gene            complement(343..507)
                     /locus_tag="VFF05_05930"
     CDS             complement(343..507)
                     /locus_tag="VFF05_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51377.1"
                     /translation="MPAGSEGLRAYRPDRDENPVAFHFFFVLYVCGGIALCVWGLLEL
                     GGMAPPLRWR"
     gene            complement(594..1385)
                     /locus_tag="VFF05_05935"
     CDS             complement(594..1385)
                     /locus_tag="VFF05_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51378.1"
                     /translation="MHRLALAAFAVLWLCGASVAQTPPTVQVLETYPAGDTVALARNQ
                     NFYLRMAYASDHPIKIWAEPYFHGEQAHAGSNPSRTYSGSGEALGWFFLMDADARVDE
                     VRIRAGDGSPSGTHEVARYAVDVHGGASTASDADKPDWVTKLSALDKAAQRADYEKRV
                     NTPPNGGDMALFGGFMLLMLALGIGGFLAPAWGLWRWRGGWRLAAAVPAVLMAFVVLR
                     IVAGTAIDPTSHNLWPFEVLSAGALSAGAMIVLAIARRIWSEPRT"
     gene            1462..>2703
                     /locus_tag="VFF05_05940"
     CDS             1462..>2703
                     /locus_tag="VFF05_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZW51379.1"
                     /translation="MSLAAKLTRHKSVEALQAELGSRRDFRRVLGVWQLTAIGLGGII
                     GVGIFVLAGQQAAANAGPAVALSFIIAGIASTAAALCYAEFAGLIPVTGSAYTYGYAV
                     LGELPAWLIGWDLLLEYALIVAAVASGWSGYVVNLLAAFGLHLPVALQGAPGTGEGHA
                     FNLVAAIAAFGVAVLLTLRTEWGARFNTAIVAVKVVAVILVIGVGAMYVDPANWHPFV
                     PDRVVDANGVGHFGYKGIATAAAVVFFAVFGYDTLTTAAEESKNPQRDLPRAILLSLG
                     ISMALYIAITLVITGMVSYTQLGNDAPVAMAFEARGMKWIGAAISATAIASILSVIFA
                     FMLAAARIWFALSRDGLLPAWFAHTHPRYGTPHRTTLALGAFTALVAGFFPIGEVAKL
                     VNIGVLSAFIVICASVIVLRRR"
BASE COUNT          452 a          931 c          878 g          442 t
ORIGIN      
        1 gtgcgcgtgc tcgatccgac cacgcgtgaa agctacgcag tcaccccgcg cgaactggcc
       61 gcgttcggca agtccgccga cacgcgggca agcttgtatc ggccatcggc gcaggcggcc
      121 ggacgccacg gcggctatcg cggccgcacc ggcatctacg aatacgtgcc ggtcgacgag
      181 gaactgcgcc gcctgatcca cgccaacgcc gccgaagcgg acatggaacg ccacgcgcgc
      241 acacgcacgc cgagcatcct cgatgacggc tggaacaaat gcatcgccgg tgtcacctcc
      301 gccgaggaag tgctgcgggt aacgcgggaa gagtagggcg gctcagcgcc accgcaacgg
      361 cggtgccatc ccgcccagtt cgagcaggcc ccacacgcac agggcgatgc cgccgcaaac
      421 gtacagcacg aaaaaaaagt ggaacgcgac gggattttcg tcgcgatcgg gccggtacgc
      481 gcgcaatccc tcgctgccag cgggcaattc gccgctgcgc cagccgttct aggcgacggc
      541 gaggatgccg ctgccgaacc ccagcagcac caggatgctc agccaagccg gcatcatgtg
      601 cgcggctccg accagatgcg gcgcgcgatc gcaagcacga tcattgcgcc ggcgctcaat
      661 gcgccggcgc tgagaacctc gaagggccac aggttgtgcg aggtcggatc gatcgcggtg
      721 ccggcgacga tgcgcaacac cacgaaggcc atcagcaccg ccggcacggc agcggccagg
      781 cgccagccgc cgcgccaacg ccacaaaccc cacgccggcg cgagaaagcc gccgatgccc
      841 aaggccagca tcagcagcat gaagccgccg aacagcgcca tatcgccgcc gttcggcggc
      901 gtgttcacgc gtttttcgta gtcggcacgc tgcgcagcct tgtcgagtgc ggacagcttc
      961 gtcacccagt cgggcttgtc cgcatcgctt gcggtggatg ctccgccatg cacgtcgacg
     1021 gcgtaacgcg cgacttcgtg cgtgccgctg ggcgagccgt ctccggcgcg gatacgcacc
     1081 tcatccaccc gtgcatcggc gtccatcaga aaaaaccagc cgagcgcctc gccgctgcct
     1141 gagtacgtgc gcgagggatt gctgccggcg tgcgcctgct cgccgtggaa atacggctcc
     1201 gcccagatct tgatcggatg gtcgctggcg taggccatgc gcagatagaa attctgattg
     1261 cgggcgagcg cgaccgtatc gcccgctgga tacgtttcca gtacctgcac ggtcggcggc
     1321 gtttgtgcga cgctcgcacc gcaaagccac agcacggcga aggcggcaag cgcaagacga
     1381 tgcattaggc ttcccccggg ctttccccaa tgcgcagcat acacggtact atcgcaccgg
     1441 ttgatccgat cgggggaacc catgagtctt gccgccaaac tcacgcgcca caaatccgtc
     1501 gaagccctgc aagccgagct cggctcgcgc cgcgatttcc gccgtgtact cggcgtgtgg
     1561 caactcaccg caatcggatt gggcggcatc atcggcgtgg gcatcttcgt gctcgccgga
     1621 cagcaggccg cggcgaacgc gggtcccgca gtcgcactga gtttcatcat cgccggcatc
     1681 gccagcacgg cggcagcgct gtgctacgcc gaattcgccg gactcatccc ggtcaccggc
     1741 agcgcctaca cctacggcta cgcggtgctc ggcgaattgc cggcgtggct gatcggctgg
     1801 gacctgctgc tcgaatacgc gctcatcgtc gccgccgtgg cgagcggctg gtcgggctac
     1861 gtggtcaacc tgctggcggc gttcggcctg catctgccgg tggccttgca aggtgcgccg
     1921 ggcacgggcg agggccacgc cttcaatctc gtcgccgcga tcgccgcgtt cggcgttgcg
     1981 gtgctgctca cgctgcgcac cgaatggggc gcgcgattca acaccgcgat cgtcgcggtg
     2041 aaggtggtcg cggtgatcct cgtgatcggc gtcggcgcaa tgtacgtcga tcccgcgaac
     2101 tggcatccgt tcgtgccgga tcgcgtcgtc gacgcgaatg gcgtgggcca cttcggctac
     2161 aagggcatcg ccaccgcggc ggcggtcgtg ttcttcgccg tgttcggcta cgacaccctg
     2221 actacggcgg cggaggaatc gaaaaatccg cagcgcgacc tgccgcgcgc gatcctgctt
     2281 tcgctcggca tctcgatggc gctgtacatc gccatcacct tggtcatcac cggcatggtg
     2341 tcgtacacgc aactcggcaa cgatgcgccg gtcgccatgg cgttcgaggc gcgcggcatg
     2401 aaatggattg gcgcggcgat ctccgcgacc gcgatcgcga gtattttgag cgtgatcttc
     2461 gccttcatgc tggcggcggc acgcatctgg ttcgcgctgt cgcgcgatgg attgttgccc
     2521 gcgtggttcg cccacacgca tccgcgttac ggcacgccgc accgcacgac gctggcgctg
     2581 ggcgcgttca ctgccctggt tgccggattc ttcccgattg gcgaggtcgc caaactcgtc
     2641 aacatcggcg tgctctcggc gttcatcgtc atctgcgcct cggtgatcgt gctgcgccgg
     2701 cgc
//
LOCUS       DASRVO010000476         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_13822, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000476 DASRVO010000000
VERSION     DASRVO010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13822"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2208)
                     /locus_tag="VFF05_05945"
     CDS             complement(<1..>2208)
                     /locus_tag="VFF05_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HZW51380.1"
                     /translation="SSYQGGHVEYFKYMVDMLRERGAGHIRVFGGGGGTITPEEIKEL
                     QAYGVERIYHPNDGMHMGLVAMIEDVVRRANEHRVAVQTPPKVSLDDEISVGKMLTAI
                     EEGVFDEAELARLRKEWQLAGGKTPVMGITGTGGAGKSSVVDELVLRFLHAFPRMRVA
                     VLAVDPTRRRSGGALLGDRIRMNSLRSHRVFMRSMATRRQHAATSIVLKDCIGFLKGQ
                     GYDLVIVETAGIGQSDSEIVDLVDFPMYVMTSDYGAASQLEKIDMLDFADLVVLNKFD
                     RRGAEDALRDVRKQWRRNRVAFKLPDEQVPVYPTIASQFNDPGVTWMFANLCRLLRDK
                     LHLDVAKWTPAVDTTLREPRATVLIPGARVRYLAEIAEQGRGINSSIDKQAEAANRAQ
                     SFWEALREIGDAKLPRELDPYAAGDVAPTAGAHMTPDSEGDRSYWETENGGTGGVDRS
                     LLALRQRYNDAVKSLSSDAIHLLREWPARLKSITDPHYLYEVRGKPVEGDNYVESLSH
                     QKVPKIAAPTLRSWGELLTFLMKENLPGAYPYTGGIYPYRRTGEDPLRMFAGEGTPER
                     TNRRFHYLSQGQKATRLSTAFDSVTLYGEDPHPRPDIYGKIGNSGVNVPTLDDMKKLY
                     SGFDLSAPNTSVSMTINGPAPIILAMFMNTAIDQQVERYLREDQARWDAAHAKIEKLY
                     EGKHRPTYTGMLPEGHDGLGLGLLGVTGDQLVDTETYAKIKAATLSAVRGTVQA"
BASE COUNT          331 a          711 c          747 g          419 t
ORIGIN      
        1 ggcctggacg gtgccgcgca cggccgacaa tgtcgccgcc ttgatcttcg cgtaggtttc
       61 ggtatcgacg agttggtcac cggtcacccc gagcaggccg aggccgaggc cgtcgtggcc
      121 ttccggcagc atgccggtgt aggtggggcg atgcttgcct tcgtagagtt tttcgatttt
      181 cgcgtgcgcg gcgtcccagc gggcctggtc ttcgcgcagg tagcgttcga cctgctggtc
      241 gatcgcggtg ttcatgaaca tcgccaggat gatcggcgcc gggccgttga tcgtcatcga
      301 caccgacgta ttcggcgccg acaggtcgaa gccggagtac agcttcttca tgtcgtccag
      361 cgtcggcacg ttgacgccgg agttgccgat cttgccgtag atgtccgggc gcggatgcgg
      421 gtcttcgccg tagagcgtga ccgaatcgaa cgcggtggac aggcgcgtgg ccttttgtcc
      481 ctgcgacagg taatggaagc ggcggttcgt gcgctcgggc gtgccctcgc ccgcgaacat
      541 gcgcagcgga tcctcgccgg tgcgccggta cggatagatg ccgccggtgt acggatacgc
      601 acccggcagg ttttccttca tcaggaaggt gagcagttcg ccccagcttc tcagcgtcgg
      661 cgcagcgatc ttcggcacct tctggtgcga gagcgactcg acgtagttgt cgccttcgac
      721 gggtttgccg cgcacttcgt acaggtaatg cggatcggtg atggatttca gccgtgccgg
      781 ccattcgcgc agcaggtgga tcgcatccga cgacagcgac ttgaccgcgt cgttgtagcg
      841 ctggcgcagc gcgagcaggg agcggtccac gccgccggta ccgccgtttt cggtttccca
      901 atagctgcgg tcgccttcgc tgtccggcgt catgtgcgcg ccggcggtcg gcgccacgtc
      961 gccggcggcg taggggtcga gttcgcgcgg cagcttcgca tcgccgatct cgcgcagcgc
     1021 ttcccagaaa ctctgcgcac gattcgccgc ctcggcctgc ttgtcgatcg atgaattgat
     1081 gccgcgaccc tgctcggcga tttcggcgag gtagcgcacg cgcgcgccgg gaatcagcac
     1141 ggtggcgcgc ggttccctga gcgtggtgtc gacagccggc gtccacttgg ccacatccag
     1201 gtgcaacttg tcgcgcagca gccggcacag gttggcgaac atccaggtca cgcccggatc
     1261 gttgaactgg ctggcgatgg tgggatacac cggcacctgc tcgtccggca gcttgaacgc
     1321 gacgcggttg cggcgccact gcttacgcac gtcgcgcaac gcgtcctcgg cgccgcggcg
     1381 gtcaaacttg ttgagcacca cgagatcggc gaagtcgagc atgtcgatct tctcgagctg
     1441 cgaggccgca ccgtagtcgc tggtcatcac gtacatcggg aaatcgacga gatccacgat
     1501 ttccgaatcg gactggccga tgccggcggt ttcgacgatg acaaggtcgt agccctgccc
     1561 cttcaaaaat ccgatgcagt ccttgagcac gatgctggtc gccgcgtgct ggcggcgcgt
     1621 ggccatcgag cgcatgaaca cccgatgcga acgcagcgaa ttcatgcgga tgcggtcgcc
     1681 gagcagcgcg ccgccggagc gccgccgcgt gggatcgacc gcgagcacgg cgacgcgcat
     1741 gcgcgggaag gcgtgcagga aacgcagcac gagttcgtcg acgacgctgg acttgcccgc
     1801 gccgccggtg ccggtgatgc ccatgaccgg cgtcttgccg ccggcgagct gccattcctt
     1861 gcgcaggcgc gcgagttcgg cctcgtcgaa aacgccttcc tcgatggcgg tgagcatctt
     1921 tccgaccgag atttcgtcgt cgagcgacac tttcggcggc gtttgcaccg ccacgcggtg
     1981 ctcgttcgcg cggcgcacca cgtcctcgat catggcgacc aggcccatgt gcatgccgtc
     2041 gttcgggtgg tagatgcgct ccacgccgta ggcctgcagc tccttgatct cttccggcgt
     2101 gatggtgccg ccgccgccgc cgaacacgcg gatgtggccg gcgccgcgct cgcgcagcat
     2161 gtccaccatg tacttgaagt actcgacgtg gccgccctgg taggagct
//
LOCUS       DASRVO010000477         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_273985, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000477 DASRVO010000000
VERSION     DASRVO010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273985"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..>1180
                     /gene="tilS"
                     /locus_tag="VFF05_05950"
     CDS             253..>1180
                     /gene="tilS"
                     /locus_tag="VFF05_05950"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZW51381.1"
                     /translation="MSASLTDLLRARLDALPPGPLAVAFSGGVDSSALLHAFAQLPAA
                     RARGLRAIHVDHGLHADSAGWASHCHDFAARLDVLLQIVAVHVDRAAGTGLEDAARRA
                     RHAAFAQSLAAGEFLALAQHRGDQAETVLLKLLRGAGPEGLGAMRSLRQYGNGYLWRP
                     LLDLPRSTLHEYARAHGLRWIEDPSNAQTHLSRNFLRHEILPRLSQHWPQAEAALAHS
                     ATWLRAAADFIDAQANAALASLRGAKPATLDWRGWLALPDALRDPVLRRWLREAGLDE
                     PAHFHVAELERQLLAANDRRPCVTFDNTQLRRH"
BASE COUNT          203 a          413 c          366 g          198 t
ORIGIN      
        1 ccgccacacc ggcaatggca gaggctttgc cgccaaaact gccgatcatc gccccgaacc
       61 ccatgaacaa caacgcgatg agaacgccga agatggccat catcaccatc tggaaaactg
      121 caagcttcaa caccgctacc cgtttgatga ccatcgcaat ctccccattg gaatgacccc
      181 gccgcaaccg cgggtgcgtc gaaactaccc gagcccaatg tcaggcgcaa ccggttctgg
      241 caccatgcgc gcatgtccgc gtcattgacc gacctgctgc gcgcccggct tgatgcactg
      301 ccaccaggcc cgcttgccgt ggctttcagc ggcggcgtgg attcgagcgc cctgctgcat
      361 gcgttcgcgc agttgcccgc ggcgcgtgcg cgcggactgc gggcaatcca tgtggatcac
      421 ggattgcacg cggatagcgc gggatgggcc tcgcattgcc acgatttcgc cgcgcggctg
      481 gatgtgctgc tgcaaatcgt cgccgtgcac gtggatcgcg ccgccggaac cgggctggaa
      541 gatgccgcgc ggcgcgcgcg ccatgccgca tttgcgcaat ccttggctgc cggcgaattc
      601 ctcgccctcg cccagcatcg cggcgaccag gccgaaaccg tgctgctcaa gcttctgcgc
      661 ggtgccggac cggaaggcct cggcgcgatg cgcagcttgc ggcaatatgg aaatggatac
      721 ctgtggcgtc ccttgctcga cctgccgcgc tcgactttgc atgaatacgc acgtgcgcat
      781 ggcctgcgct ggatcgagga tccgagcaac gcgcagacgc atctgtcgcg caatttcctg
      841 cgtcatgaaa tcctgccgcg cctgagccaa cactggccgc aggccgaagc ggcgctggcg
      901 cacagcgcga cgtggttgcg cgccgccgcg gatttcatcg atgcccaggc caacgccgcg
      961 ctggcaagct tgcgcggagc caaaccggca acgctggatt ggcgcggctg gcttgccctg
     1021 ccggatgcct tgcgcgatcc ggtgctgcgg cgctggctgc gcgaggcggg tttggacgaa
     1081 cccgcgcact tccacgtcgc cgaactcgaa cgccagctgc ttgccgcgaa cgatcgccgg
     1141 ccctgcgtta ccttcgacaa cacgcaattg cgccggcatc
//
LOCUS       DASRVO010000478         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_221876, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000478 DASRVO010000000
VERSION     DASRVO010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221876"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFF05_05955"
     CDS             <1..279
                     /locus_tag="VFF05_05955"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448608.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZW51382.1"
                     /translation="VFENRFMHVHELERLGADIRLDGNTAVVKGVREMSGAPLMATDL
                     RASACLVLAGLVAKGDTTVDRIYHIDRGYECIEEKLGTLGAKIRRLPS"
     gene            complement(281..970)
                     /locus_tag="VFF05_05960"
     CDS             complement(281..970)
                     /locus_tag="VFF05_05960"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HZW51383.1"
                     /translation="MRSLLFAACLLVLPTLADAGATLQPETANYTVLRDGKAIGTAMY
                     ALSANADGTWTLHSETRGTGGMAKLLGLDVREDSVFGFHDGKLQGLRYDYRQDAAIKH
                     KQRSIDFDAKAGQIRVRDNGKGFRYALVDGAIDRSTVAVALGLALANGAKSAMLPVAV
                     RDRVEMQQYAIRGGAVINLPGGRYEATELDRTDAPGKVMKSWYAQGLLPVRVEQPQHD
                     GKAIVLELKPR"
     gene            1348..>2616
                     /locus_tag="VFF05_05965"
     CDS             1348..>2616
                     /locus_tag="VFF05_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51384.1"
                     /translation="MAVLLLPGIASALPTFNRQTGQNCQACHAGGQFPELTAYGRLFK
                     LTGYTLGARTLPFAGMAVLSDSHVNNTSKSDDPKADFQKNDQPIFATASVFAAGKITD
                     NLGGFLQVTYDNYASQSDDGHFHGHTNVDNVDIRYADRSIDANHDVIWGVSTNNNPSV
                     SDVWNTAAAWMQYVPVPSPTSSRFIDGSAPYPGFAAGGNLAGLTGYLFWNQTLYVETG
                     GYRSATGPASFMSAGVADGDKTKLQGTNPYWRIALSHDWGPHSLMVGTSGMNADVYDD
                     PLDTSDPSALHHFRDLGVDAQYQYLLDPHTFTAQLAYLRDRQRLPAFLAGQPVFDVNG
                     NPLPDTNAVDRTNVFRAKASYVYDAHYGGSLSFFNQTGTTDSALYDPTRVSGNISGNP
                     AVRGWTYEAFWTPVQYMRIGLQYTRYDKYNG"
BASE COUNT          487 a          856 c          803 g          470 t
ORIGIN      
        1 gtgttcgaga accgcttcat gcacgtgcac gaactcgagc gcctcggcgc cgacatccgc
       61 ctggatggca acaccgccgt ggtcaagggc gtgcgcgaga tgtccggcgc gccgctgatg
      121 gccaccgacc tgcgcgcgtc cgcgtgcctg gtgctggccg gactcgtcgc caagggcgac
      181 accacggtcg accgcatcta ccacatcgac cgcggttacg agtgcatcga ggaaaaactc
      241 ggcacgctgg gagcgaagat ccggcgcctg ccgtcttgaa tcagcggggc ttgagctcca
      301 acacaatcgc tttgccatcg tgctgtggct gctccacgcg taccggcagc agaccttgcg
      361 cgtaccacga tttcatcacc ttgcccggcg cgtcggtgcg gtcgagttcg gttgcctcgt
      421 agcgcccacc cggcaggttg atcacggcgc caccgcgtat cgcgtattgc tgcatctcca
      481 cacggtcgcg taccgcgacc ggcagcatcg ctgacttcgc accattggcc agcgccagtc
      541 ccagcgcgac cgccaccgtg ctgcggtcga tggcgccgtc cacgagtgcg tagcgaaaac
      601 ccttgccgtt gtcgcgcacg cggatttgcc cggcttttgc gtcgaaatcg atgctgcgct
      661 gcttgtgctt gatcgcggca tcctgccgat agtcgtagcg caggccttgc agcttgccat
      721 cgtggaagcc aaagacggaa tcctcgcgca cgtccaggcc gagcagtttc gccatgccgc
      781 cggtaccgcg agtttcgctg tgcaatgtcc aggtgccgtc ggcgttcgcg ctgagcgcat
      841 acatcgccgt gccgatggcc ttgccatcgc gcagtacggt gtagttcgct gtttccggct
      901 gcagggttgc accggcgtcg gcgagcgtgg gcaggacgag caggcaggcg gcgaaaagca
      961 ggctgcgcat gggatcggcg ggtaatgaag gtgcacgcag tgtggacgcg ggcgcctgaa
     1021 cgacatccga ccatatgggc ggcggcgacc tgattttccg cagcgggctc gaataacgcc
     1081 gtgcggtcgc gggtaccgcg ccctgtgcga attgatgcaa gtcaagtgcg gtgtcgccgt
     1141 cgatggaata tattacgccg cccgctgccg aacgcagtgt cacgcatttt gcggaacggt
     1201 cttaccagca ggctgctcgg gatgtttgtc gcggcgacgc ggacttccct tcatcgcgat
     1261 gtccaggcgg aatgcgggag gctgaacatg atctgcagac ccattcgcag cattcgacgc
     1321 ggcgcaagcc gaacgctcgg aggattgttg gcggttctgt tacttcccgg gatcgccagc
     1381 gcgttgccga cgttcaatcg ccagaccggg caaaactgcc aggcatgcca cgccggtggg
     1441 caatttccgg aactgaccgc gtacggacgc ctgttcaaat tgaccggcta tacgttgggc
     1501 gcgcgcacgc tgccgttcgc cggcatggcg gtactgagcg attcgcacgt caacaacacg
     1561 agcaaaagcg acgatcccaa agccgatttc cagaagaacg atcagccgat cttcgcaact
     1621 gcgagcgtgt tcgcggccgg caagatcacc gacaaccttg gcgggttcct gcaagtcacc
     1681 tacgacaact acgccagcca gtcggacgat ggccatttcc acggccatac caatgttgac
     1741 aacgtcgaca tccgctacgc agaccgcagc atcgacgcaa accacgacgt gatctggggt
     1801 gtcagcacga acaacaatcc gtcggtatcg gacgtctgga acactgcggc cgcatggatg
     1861 cagtacgtac cagtgccgag tcccaccagc agccgcttca tcgacggcag tgcaccttat
     1921 ccgggttttg cggcgggcgg caatctcgcc ggcctgaccg gttacttgtt ctggaaccag
     1981 accctgtacg tcgaaaccgg cggctaccgc agcgccacgg gcccggcatc gttcatgagc
     2041 gccggcgtcg ccgacggcga caaaaccaag ctgcagggca ccaatccgta ctggcgtatc
     2101 gcactgtcgc acgattgggg cccgcacagc ctgatggtcg gcacgtcggg catgaacgcc
     2161 gacgtatacg acgatcccct cgacacatcg gacccgtcgg cactgcatca tttccgtgat
     2221 ctgggcgtcg acgcgcaata ccagtacctg ctagacccgc acaccttcac cgcgcaactc
     2281 gcctatctgc gcgaccgcca gcgcctgccg gcatttctgg ccgggcagcc ggtcttcgac
     2341 gtgaacggca acccgttgcc ggacacgaat gcagtggacc gcaccaatgt gttccgcgcc
     2401 aaggcgagct acgtctacga cgcgcactat ggcggcagtc tgtctttctt caaccagacc
     2461 ggcacgaccg attcggcgtt gtacgacccg acgcgggtgt ccggcaacat ctccgggaat
     2521 ccggccgtgc gcggctggac ttacgaggca ttctggactc cggtgcagta catgcgcatc
     2581 ggcttgcagt acacgcgcta cgacaagtac aacggc
//
LOCUS       DASRVO010000479         1627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_40600, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000479 DASRVO010000000
VERSION     DASRVO010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40600"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /gene="folK"
                     /locus_tag="VFF05_05970"
     CDS             <1..412
                     /gene="folK"
                     /locus_tag="VFF05_05970"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZW51385.1"
                     /translation="LAQLRKTRLVARSCLYRSAAWGRADQPEFINAAAALETDLSPRE
                     LLDALLALERDAGRERDGTRWGPRVLDLDILLFGDRHIDEPGLHVPHPHLHQRAFVLV
                     PLAEIAPDLVIAGHGRLADLLACVDVSSCRRMAE"
     gene            436..1257
                     /gene="panB"
                     /locus_tag="VFF05_05975"
     CDS             436..1257
                     /gene="panB"
                     /locus_tag="VFF05_05975"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZW51386.1"
                     /translation="MYANAKSAARAAVTVPQLHAMKARSEKIVMLTAYDASFAVQCDA
                     AGVDAVLVGDSLGMVVQGHASTLPVSVDDMVYHTAAVARGTASALLIADMPFMSFRDA
                     EAALRSAGRLLAEGGAAMVKLEGAGYVLDVIAALARHAIPVCAHLGLTPQSVHKLGGY
                     RVQGKGKDAAAQLLADARAVADAGADLLVLECVPATLAKRITNEIPIPTIGIGAGADC
                     DGQVLVLYDMLGITPGKRPRFSKDFLAGGGSVGEAIRAYAEAVRDGTFPAAEHAF"
     gene            1257..>1627
                     /locus_tag="VFF05_05980"
     CDS             1257..>1627
                     /locus_tag="VFF05_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZW51387.1"
                     /translation="MDTFHSAADLRACVSAWRREGRRIAFVPTMGNLHAGHFSLIELA
                     RAHADKIVASVFVNPTQFGPNEDFASYPRTLEADRAGLAAQGCDALFAPSVDEMYPFG
                     AAASVRVEVPKITEVLEGELRP"
BASE COUNT          263 a          527 c          536 g          301 t
ORIGIN      
        1 actcgcgcag ttgcgcaaga cccggctcgt cgcacgttcg tgcttgtatc gcagcgccgc
       61 gtggggacgc gccgatcaac ccgaattcat caacgcggcg gcagcactgg aaacagattt
      121 gagtccgcgc gaactgctcg atgcattgct cgcgctcgaa cgtgacgccg ggcgcgagcg
      181 cgacggcacg cgctgggggc cgcgcgtact cgacctggat attctcttgt tcggcgatcg
      241 ccatatcgac gaacccggct tgcatgtgcc gcatccgcac ctgcatcaac gcgccttcgt
      301 gctcgtgccg ctggccgaaa tcgcgccgga cctggtcatt gccggccatg gccgcctcgc
      361 agatttgctc gcatgcgtcg atgtttcgtc ctgcaggcgc atggcagaat agccgttcgg
      421 cttcgggagt ccgcgatgta cgccaacgcc aaatccgccg cgcgcgcagc cgtcactgtg
      481 ccgcagttgc acgcgatgaa agcgcgcagc gagaaaatcg tcatgctgac cgcctacgac
      541 gccagcttcg ccgtgcagtg cgacgccgcc ggcgtcgatg ccgtgctggt tggcgattcg
      601 ctcggcatgg tcgtgcaggg tcacgcgagc accttgccgg tcagcgtcga cgacatggtc
      661 tatcacaccg cggcggtggc gcgcggcaca gcaagcgcct tgctcattgc cgacatgccg
      721 ttcatgagct ttcgcgatgc ggaggcggcc ctgcgcagcg ccggacgcct gctcgccgaa
      781 ggcggcgcgg cgatggtcaa gctcgaaggc gctggctatg tgctcgacgt gatcgctgct
      841 cttgcgcggc acgcgattcc ggtttgcgcg cacctgggcc tgaccccgca atccgtgcac
      901 aagcttggcg gctatcgcgt gcagggcaag ggcaaggatg ccgccgcgca acttctggcc
      961 gatgcgcgcg cggtcgccga tgccggtgcc gatttgctcg tgctcgaatg cgtgccggcg
     1021 acgctggcca aacgcattac caacgagatt ccgattccga ccatcggcat cggtgccggc
     1081 gcggattgcg atggccaggt gctggtgttg tacgacatgc tcggcatcac gcccggaaaa
     1141 cgaccgcgct tttccaagga ttttctcgcc ggcggcggat cagtgggcga ggcgatccgg
     1201 gcctatgccg aagcggtgcg tgacggaacg tttccggcgg cggagcacgc gttctgatgg
     1261 atacattcca ttccgcggcg gacttgcgcg cttgtgtatc tgcgtggcgc cgcgaaggca
     1321 ggcgcatcgc ctttgtaccg acgatgggca atctgcatgc aggccatttt tcgctgatcg
     1381 agttggcgcg tgcgcacgcc gacaagatcg tcgccagcgt gttcgtcaat ccaacccagt
     1441 tcggccccaa cgaggatttc gcgagctatc cgcgcacgct cgaggcggat cgtgccggac
     1501 tcgctgcgca gggctgcgac gcattgttcg cgccaagcgt cgacgagatg tatccgtttg
     1561 gcgccgccgc aagcgtgcgc gtggaggttc cgaaaatcac cgaagtcctg gaaggcgaac
     1621 tgcgccc
//
LOCUS       DASRVO010000480         1373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_221914, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000480 DASRVO010000000
VERSION     DASRVO010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221914"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1373
                     /locus_tag="VFF05_05985"
     CDS             <1..>1373
                     /locus_tag="VFF05_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW51388.1"
                     /translation="LDVRDKLDLLQLPLTAKKPVLLRFNPATDPILRLALTGPSESGT
                     AENAELKQLRRFADDELRKRLEPTSGVAAVKVGGGLEDQIDVDIDQQRLQQLNLNVAD
                     VIKRLGDENVNVSGGRIEDGTQRYLVRTVNQFTDLGQMSDLLIKVDKGVPIRLKDVAN
                     VHQGHKEREGIVRVDGHEAVELAIYKEGDANTVTVAAAVRKQLDRFTCANQPPSCTKI
                     LPPNAKLTMIDDQSVFIQSSLNDVRDDAIIGGVLAVLIIFFFLADSWSTFIISLSLPV
                     SLIATFFFMGEAGLSLNVMSLGGLALATGMVVDDSIVVLENIARMRERGIGIVESAVQ
                     GAREVSMAVTASTLTTVAVFFPLVFVQGIAGQLFRDQALTITFAMLISLVVAMSLIPM
                     LASLKGRAPLAFADETKAPRVPPRTRIGKFFAAIGRLFRWLPRAFGWVGFTLARLGGT
                     VIGRILR"
BASE COUNT          243 a          431 c          438 g          261 t
ORIGIN      
        1 ctggacgtgc gcgacaagct cgacctgctg caactgccgt tgaccgcgaa gaaaccggtg
       61 ctgttgcgct tcaatccggc gaccgatccg atcctgcgcc tggcgctgac cggcccatcg
      121 gaaagcggca cggcggagaa cgccgaattg aagcagttgc gccgctttgc cgacgacgaa
      181 ctgcgcaaac gactggagcc gacttccggc gtggccgcgg tcaaagtcgg cggcggactc
      241 gaggatcaga tcgacgtcga catcgaccag cagcgcctgc agcaactcaa tctcaatgtc
      301 gccgacgtga tcaagcgtct tggcgacgaa aacgtgaacg tatccggcgg gcgcatcgag
      361 gacggcactc agcgttatct cgtgcgtacg gtcaaccagt ttacggacct tggccagatg
      421 agcgacctgc tgatcaaggt cgacaagggc gttccgatcc ggctcaagga tgtggcgaac
      481 gtgcaccagg gccacaagga gcgcgaaggc atcgtgcgcg tggacgggca cgaggcggtg
      541 gagctggcga tctacaagga aggcgatgcc aacaccgtca cagtggccgc ggcggtgcgc
      601 aagcagctcg acaggttcac ctgcgcgaac cagccgcctt cctgcacgaa gatcctgccg
      661 ccaaacgcga agctgaccat gatcgacgat cagtccgtct tcatccagag ctcgctcaac
      721 gacgtgcgcg acgacgccat catcggcggc gtgcttgcgg tgctgatcat tttcttcttc
      781 ctcgccgact cgtggagcac attcatcatt tcgttgtcgc tgccggtgtc gctgatcgcc
      841 acgttcttct tcatgggcga agccggcctg agcctcaacg tgatgtcgct gggcggatta
      901 gcgctcgcca ccggcatggt tgtggacgat tctatcgttg tactcgaaaa catcgcgcgc
      961 atgcgcgagc gcggcatcgg tatcgtcgaa tcggcggtgc agggcgcgcg cgaggtgtcg
     1021 atggcggtga ccgcctcgac gctgaccacg gtcgcggtgt tcttcccgct cgtgttcgtg
     1081 cagggcatcg ccggccagct gttccgcgat caggcgctga cgatcacgtt cgccatgctg
     1141 atttcgctgg tcgtggcgat gagcctgatt ccgatgttgg cttcgctcaa gggcagggcg
     1201 ccactggcgt tcgccgacga aaccaaagca ccccgtgtgc cgccgcgcac gcgcatcggc
     1261 aaattcttcg cagcgatcgg ccgcttgttc cgctggttgc cacgcgcctt cggctgggtc
     1321 ggttttacgc tggcgcgcct cggcggcacc gtcattggcc ggattctgcg ctt
//
LOCUS       DASRVO010000481         1943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_40663, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000481 DASRVO010000000
VERSION     DASRVO010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40663"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFF05_05990"
     CDS             complement(<1..329)
                     /locus_tag="VFF05_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZW51389.1"
                     /translation="MNANAKTVSLVLGSGGARGYAHIGAIEELLAQGYDIRSVAGASM
                     GALVGGIYAAGKLDDYRDWARGLQKFDVLRLLDWTFRGGLIKGDRIIGRLKDLIGEVA
                     IENLPIP"
     gene            complement(326..1294)
                     /locus_tag="VFF05_05995"
     CDS             complement(326..1294)
                     /locus_tag="VFF05_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HZW51390.1"
                     /translation="MDRYERILTLHRILKSSRYPVPLARLMDELGCSRATAYRDIAFL
                     RDALGAPIDSEGDEAAFHYASAEAERFELPGLWLTSEELQALLALNELVGRTGPGILA
                     SALAPLRSRIDRLLSNQTDGQSFPVERIRVIGSGTRKVDEVTFRHVAGAVLGRRRLKF
                     DYRARSTNAATTRSVSPQRLTHYRDNWYLDAWDHGRDALRSFALDRMGRASVLDEAAE
                     DRDTAELDAHLASSYGIFSGAPKATATIRFSAHAARWVADEHWHSQQQGMRLPDGRYE
                     LKVPYSNSRELLMDVLRYGPDAEVVAPVSLREEMKIMLGLALGAYK"
     gene            1348..>1943
                     /locus_tag="VFF05_06000"
     CDS             1348..>1943
                     /locus_tag="VFF05_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZW51391.1"
                     /translation="MPHANLKLHEPVGDEVKTTTCYMCACRCGIKVHLKDGRVRYIQG
                     NPAHPVNKGVLCAKGSAGIMQHYSPARLRKPLLRTGARGEGEFREIEWDEALALATKW
                     LGDIRARNPDELAFFTGRDQSQALTGWWAQQFGTINYAAHGGFCSVNMAAAGMYTLGG
                     SFWEFGEPDWDRTKYLLMFGVAEDDDSNPIKLGLGKLKA"
BASE COUNT          339 a          630 c          658 g          316 t
ORIGIN      
        1 tagggaatcg gcagattttc gatcgcgacc tcgccgatca ggtccttgag ccggccgatg
       61 atgcggtcac ccttgatcag gccgccgcgg aaggtccagt cgagcaggcg cagcacgtcg
      121 aatttctgca acccgcgcgc ccagtcgcga taatcgtcga gcttgcccgc cgcatagatg
      181 cctccgacca gcgcgcccat cgaggcgccg gccacgctgc ggatgtcgta gccctgggca
      241 agcaattcct cgatcgcgcc gatgtgcgca tagccgcgcg cgccgccgga gccgagcacg
      301 agggaaacgg ttttcgcatt cgcgttcatt tgtaggcgcc cagcgcgagt ccgagcatga
      361 tcttcatttc ctcacgcaag ctgaccggtg cgacgacttc cgcgtccggg ccgtagcgca
      421 gcacgtccat cagcagttcg cgcgagttcg aatacggtac tttcagttcg taacggccgt
      481 cgggcaggcg catgccctgc tgctgcgaat gccagtgttc gtcggcgacc cagcgcgcgg
      541 cgtgcgcgga aaaacggatc gtcgcggtgg ccttgggcgc accggaaaag atgccgtagc
      601 tcgaggccag atgcgcgtcg agttcggccg tgtcgcgatc ctcggcggct tcgtcgagca
      661 cgctggcgcg gcccatgcgg tcgagcgcga aactgcgcag cgcgtcgcgg ccgtgatccc
      721 aggcgtccag ataccagttg tcgcgataat gggtcaggcg ctgcggcgaa acgctgcgcg
      781 tggtcgccgc gttggtcgag cgcgcacgat agtcgaattt caggcggcgc cgaccgagca
      841 ccgcaccggc gacatggcga aacgtcacct cgtccacttt gcgcgtgccc gagccgatca
      901 cgcggatgcg ctcgacgggg aaactctggc catcggtctg gttcgacagc agccggtcga
      961 tgcgcgagcg caacggcgcc agcgcgctgg cgaggatgcc gggaccggtg cggccgacca
     1021 gttcgttgag cgcaagcagg gcctgcaatt cttcgctggt cagccacagg ccgggcagct
     1081 cgaagcgctc ggcctcggcg ctggcgtagt ggaacgccgc ttcgtcgccc tcgctgtcga
     1141 tgggcgcgcc gagcgcatcg cgcaggaagg cgatgtcgcg ataagccgtg gcgcgcgagc
     1201 agcccagttc gtccatcaac cgcgccagcg gtaccggata gcgcgacgac ttcaggatcc
     1261 ggtgcagggt gaggatgcgc tcatatctgt ccatggcctt atggtacggc atgcgggccg
     1321 ccgcgccgca tccgttatca tgcgcgcatg ccgcatgcca acctgaaatt gcacgagccc
     1381 gtcggggacg aggtcaagac cacgacgtgc tacatgtgcg cctgccgctg cggcatcaaa
     1441 gtgcatctga aagacggtcg cgtgcgttac atccagggca atcccgcgca cccggtcaac
     1501 aagggcgtgc tctgcgcgaa gggctcggcc gggatcatgc agcactactc gccggcgcgc
     1561 ttgcgcaagc cgctgttgcg cacaggggcg cgcggcgaag gcgaattccg cgagatcgaa
     1621 tgggacgagg cgctggcgct ggcgacgaaa tggctcggcg acatccgcgc acgcaatccg
     1681 gacgagctcg cgttcttcac cggacgcgac cagtcgcagg cgctgaccgg ctggtgggca
     1741 cagcagttcg gcacgatcaa ttacgcagcg cacggcggct tctgctcggt caacatggcc
     1801 gcagcgggca tgtacacgct cggcggttcg ttctgggaat tcggcgagcc ggactgggac
     1861 cggacgaaat acctgctcat gttcggcgtc gccgaggatg acgactccaa cccgatcaag
     1921 ctgggactcg gcaagctcaa ggc
//
LOCUS       DASRVO010000482         1307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_274138, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000482 DASRVO010000000
VERSION     DASRVO010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274138"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VFF05_06005"
     CDS             <1..174
                     /locus_tag="VFF05_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HZW51392.1"
                     /translation="HAGRAAAVEPYADWFGERWFVLPNPTYGSWEPALFNNDWTLPRA
                     KRDAAKRGALRTD"
     gene            273..1211
                     /locus_tag="VFF05_06010"
     CDS             273..1211
                     /locus_tag="VFF05_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51393.1"
                     /translation="MSKLVRFALVAALAAAGFGCSQQQNAANPAAPTAAVAPTTPDAA
                     VAVSIKALRDNNIAALIENALPAPEAVKLKADWNKDMNSEPITDEDRKEFAEKMAALT
                     APDAEAKMYAQIQPKLAEFDKQSAQQMPMMIAMGQGFAKSAIAQNKDLSDAQKKQAED
                     LLDATAQWAQTTKFTDPALVKAAIAEICKTARAVNLKTADEARALSYEQAMQKAGIVL
                     GGVKAVLAVYGLNIDKTLDSVKIDAGAASGDAATVTVTYVAFDKPFTTEAQMVKVDGR
                     WYGKHAIDQWHKHQAEIAAAGKTAAPAEPAPAEAGK"
BASE COUNT          268 a          433 c          414 g          192 t
ORIGIN      
        1 catgccggcc gtgccgccgc ggtcgagcct tacgccgact ggttcggcga gcgctggttc
       61 gtgctgccca atccgaccta tggttcgtgg gagccggcat tgttcaacaa cgattggacg
      121 ctgccccgcg ccaagcgcga tgccgccaag cgcggcgccc tgcgcaccga ttgaacgcag
      181 cctgaccgac aatgcgggcg tgcttgcctt ggcagcgtgc gctgactaaa gtcgccatcc
      241 cgcaccgcca accgctcctt ttggggaaga tcatgtccaa gcttgtccgt ttcgccctcg
      301 tcgccgcgct cgcggccgcc ggtttcggct gcagccagca gcagaatgcc gccaacccgg
      361 ctgcgcccac cgccgcggtc gcgccgacta cgccggatgc cgccgttgcc gtctcgatca
      421 aggcgctgcg cgacaacaac atcgccgcgc tgatcgaaaa cgcactgccc gcgccggaag
      481 cggtcaagct caaggctgac tggaacaagg acatgaattc cgagccgatc acggacgaag
      541 accgcaagga attcgccgaa aagatggcag cgcttaccgc gccggacgcc gaggccaaga
      601 tgtacgcgca gatccagccg aagctggccg aattcgacaa gcagtccgcg cagcagatgc
      661 cgatgatgat cgcgatgggc cagggtttcg ccaagagcgc gatcgcgcag aacaaggacc
      721 tgtccgacgc gcagaagaaa caggccgaag acctgctcga tgcgaccgcg cagtgggcgc
      781 agacgaccaa gttcacggac cccgccctgg tcaaggcggc gatcgcggaa atctgcaaga
      841 cggcgcgtgc ggtgaacctg aagaccgccg atgaggcacg cgcgctcagt tacgagcagg
      901 ccatgcagaa agccggcatc gtgctcggcg gcgtcaaggc ggtgctcgcc gtgtacggtc
      961 tgaacatcga caagacgctg gattccgtga agatcgacgc gggcgctgcc agcggcgacg
     1021 cggccacggt cacggtcacg tatgtcgcgt tcgacaagcc gtttaccacc gaagcgcaaa
     1081 tggtcaaggt cgacggacgc tggtatggca agcacgccat cgaccagtgg cacaagcatc
     1141 aggcggaaat cgccgccgcg gggaaaacgg cggcacccgc agagccggca ccggccgaag
     1201 ctggcaagta attccgcttt tccgatgttt tgcatttggc ccgccgcgag cgggccaaat
     1261 gctttctgaa caaccccgtc ccgtacaatg cgggtatgga cgctccc
//
LOCUS       DASRVO010000483         1337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_117665, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000483 DASRVO010000000
VERSION     DASRVO010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117665"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFF05_06015"
     CDS             complement(<1..804)
                     /locus_tag="VFF05_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW51394.1"
                     /translation="MKLEKRTILRVVIIVAALGYLAFTHFAKTQAGAKHAASLAAPIP
                     ANAKAFTLGTLAFKSCELPQKHSGATTSAFCAPFAVPENRAETNGRKLDLRLALIKSD
                     AVTADSDIVVFLAGGPGQAAIDTWPQIAPAFAPLLAHHHVLLLDQRGTGGSNVLECKG
                     DEADEGESDNDTGFDAAKAKQRAQECLDQVSKHADPRFYTTTDAVADLEAVRQALGAP
                     QFDLVGVSYGTRMAQQYAMRHADGVRSIVLDSVAPNEIALGQDFAENLDN"
     gene            complement(801..1001)
                     /locus_tag="VFF05_06020"
     CDS             complement(801..1001)
                     /locus_tag="VFF05_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZW51395.1"
                     /translation="MKNRVRELREKRDWSQGNLAEKLDVSRQTVNAIETGKYDPSLPL
                     AFKIAKLFGQRIEALFEYEEEK"
     gene            complement(998..>1337)
                     /locus_tag="VFF05_06025"
     CDS             complement(998..>1337)
                     /locus_tag="VFF05_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51396.1"
                     /translation="VSGFLAAAHVFPPEWAAEALVWIFPLLMLCYGGAQLYAARRYGG
                     GVCDEGGMPVAIRFLYAATLCACIAAYFYFRKGDRDASATMLGMAGGMAVFAGGFAVW
                     RWLRGRSMRE"
BASE COUNT          238 a          448 c          398 g          253 t
ORIGIN      
        1 gttgtctaga ttctcggcga agtcctggcc caacgcgatc tcgttcggcg ccacggaatc
       61 gagcacgatg ctgcgcacgc catccgcgtg gcgcattgcg tactgttgcg ccatgcgcgt
      121 gccgtaggac acgccgacca ggtcgaactg cggagcgccc agcgcctggc gcacggcttc
      181 cagatccgcg accgcgtcgg tggtcgtgta gaaacgcgga tcggcgtgtt tggaaacctg
      241 atccaggcat tcctgggcgc gctgtttcgc cttggcggcg tcgaaaccgg tgtcgttatc
      301 cgattcgccc tcgtctgcct cgtcgccctt gcattccagc acgttggaac cgccggtacc
      361 gcgctggtcg agcagcagaa cgtggtgatg cgccaacaac ggcgcgaacg ccggcgcgat
      421 ctgcggccag gtgtcgattg cggcctgtcc cggcccgccg gcgaggaaca cgacgatgtc
      481 gctgtcggcc gtgacggcat cggacttgat cagcgccagg cgcagatcga gcttgcgtcc
      541 atttgtctcg gcccgattct cgggcaccgc aaacggcgcg cagaacgcac tcgtcgtcgc
      601 gccggaatgc ttttgcggca attcgcagga cttgaacgcc agcgtgccga gcgtgaaggc
      661 tttcgcattc gctggaatcg gcgcggccag acttgccgcg tgttttgcgc cggcctgcgt
      721 cttggcgaaa tgcgtgaacg ccagatagcc gagcgcagcc acgatgatga ccacgcgcaa
      781 gatggttcgt ttctccagtt tcatttctcc tcctcatact cgaacagcgc ttcgatgcgc
      841 tgcccgaaca atttcgcgat cttgaatgcc aacggcaggc tcgggtcgta cttgccggtt
      901 tcaatcgcgt tcacggtttg acgcgagaca tccaacttct cggccagatt gccctgcgac
      961 cagtcccgct tctcgcgcag ttcgcgcacc cggtttttca ttcgcgcatc gagcggccgc
     1021 gcaaccaacg ccaaaccgca aatcctccgg cgaacaccgc catgccaccg gccatgccca
     1081 gcattgtcgc gctggcatcc ctgtcgcctt tgcggaaata gaagtacgca gcgatgcagg
     1141 cgcacagggt tgccgcatac agaaagcgga ttgccaccgg catgccgcct tcgtcgcaga
     1201 caccgccacc gtatcgccgc gccgcataaa gttgtgcacc gccgtagcac agcatcagca
     1261 gcgggaatat ccagaccagc gcttccgccg cccactcggg cggaaacacg tgtgcggctg
     1321 ccagaaatcc actgacg
//
LOCUS       DASRVO010000484         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_222079, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000484 DASRVO010000000
VERSION     DASRVO010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222079"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VFF05_06030"
     CDS             <1..1117
                     /locus_tag="VFF05_06030"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003476792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HZW51397.1"
                     /translation="RRLAQCRLPRFLFDYIDGAAGDEITLAANVADLAAIRLRQRVLV
                     NVENVDTRATLAGEDCAMPLALAPVGMAGMMARRGEAQAVRAANAAGVPFTLSTVGIC
                     PLAEVQAASRAPFWFQLYMIRDRDVIVSLLERAWAAGCRTLVFTIDLPIPGMRHRDTR
                     NGVGGKGLRAALLKAAQVLPRPHWLWNVALRGRPLSFGNLTDQVPGARDLTAFWAWVN
                     SQFDTRVTWRDIEWLRAHWKGRLLLKGILDADDARAAVDVGADGIVVSNHGGRQLDGA
                     ASSISKLSAVVQAVGNKTEVLLDGGVRSGADIFKAVALGARGVLIGRPWVWALAGAGE
                     SGLRDLLATLQRELKLTMTMAGVTRIADIGVNHLDKV"
     gene            1138..>1521
                     /locus_tag="VFF05_06035"
     CDS             1138..>1521
                     /locus_tag="VFF05_06035"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZW51398.1"
                     /translation="MANGAQALIKTLADAGIEVCFSNPGTSEMHFVAALDSEPRLRAV
                     LALFEGVATGAADGYARMAGKPAATLLHLGCGLGNGLANLHNARKGRVPVLNIVGDHA
                     TYHVQYDAQLQSDIETVARNVSPGFV"
BASE COUNT          229 a          515 c          522 g          255 t
ORIGIN      
        1 ccgccgtctc gcgcaatgcc gcctgccgcg cttcctgttc gactacatcg acggcgctgc
       61 cggcgacgaa atcaccttgg cagccaatgt cgcggatctt gccgcgatcc gcttgcgcca
      121 gcgcgtgctg gtcaacgtgg aaaatgtgga cacgcgcgcc acgctcgccg gcgaggattg
      181 cgcgatgccg ctggcgctcg ccccggtggg catggcgggc atgatggcgc ggcgcggcga
      241 agcgcaggcg gtgcgtgcgg cgaatgccgc gggcgtgccg ttcacgctgt ccacggtcgg
      301 catctgtccg ctggccgagg tgcaggccgc gagccgcgcg ccgttctggt tccagctcta
      361 catgattcgc gaccgcgacg tcatcgtctc cctgctcgaa cgcgcctggg ccgccgggtg
      421 ccgcacgctg gtcttcacca tcgacctgcc gatccccggc atgcgccacc gcgatacgcg
      481 caacggtgtc ggcggcaagg gcctgcgcgc ggcgctgctc aaggcggcgc aagtgctgcc
      541 gcgcccgcac tggctgtgga acgtggcgct gcgcggccgg ccgttgagct tcggcaacct
      601 gaccgatcag gtgcccgggg cgcgcgacct cactgcgttc tgggcctggg tgaattcgca
      661 gttcgatacc cgcgtgacct ggcgcgacat cgagtggctg cgcgcacact ggaaaggccg
      721 cctgctgctc aagggcatcc tcgacgccga cgatgcgcgc gcggccgtgg acgtcggcgc
      781 ggacggcatc gtcgtctcca accacggcgg ccgccagctc gatggcgcgg cctcgtcgat
      841 ttcgaaattg tcagcggtcg tgcaggcagt tggcaacaag accgaagtat tgcttgatgg
      901 cggcgtgcgc agtggcgctg acatcttcaa ggccgtcgcg ctcggcgcgc gcggtgtact
      961 gatcggtcgc ccctgggtgt gggcgctggc cggtgcgggc gaatcaggtt tgcgcgacct
     1021 gctcgccacc ttgcagcgcg aattgaaact gacgatgacg atggctggcg tcacccgcat
     1081 cgccgacatc ggcgtgaacc atctcgacaa ggtttgacga cttcgaagga aatctccatg
     1141 gccaacggcg cacaagccct gatcaagacc ctggccgatg ccggcatcga ggtgtgcttc
     1201 tccaatcccg gtacgtcgga gatgcatttc gtcgcggcac tggacagcga accgcgcctg
     1261 cgcgcggtgc tcgccctgtt cgaaggcgtg gccaccggcg cggccgacgg ctatgcgcgc
     1321 atggccggca agccggccgc gaccttgctg catctgggct gcggcctcgg caatggcctg
     1381 gccaacctgc acaacgcgcg caagggtagg gtgccggtgc tcaacatcgt cggcgaccac
     1441 gcgacgtatc acgtgcagta cgacgcgcag ttgcaatccg acatcgaaac cgtcgcgcgc
     1501 aatgtctcgc cgggattcgt g
//
LOCUS       DASRVO010000485         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_351040, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000485 DASRVO010000000
VERSION     DASRVO010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351040"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFF05_06040"
     CDS             <1..441
                     /locus_tag="VFF05_06040"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HZW51399.1"
                     /translation="AAAYAQWLGKQTGKRYRLASPAEFDRVARQAARGDCKANLADAA
                     YKRKFDSRDGSNCDDGFAATSPVGKFPAADGVYDIDGNVREWVAACSGGAPAQAGSDC
                     RSFMVKGRGWLSQAARESPTFSDTYSGDVALNSVGFRVVRDVDK"
     gene            499..>1126
                     /locus_tag="VFF05_06045"
     CDS             499..>1126
                     /locus_tag="VFF05_06045"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZW51400.1"
                     /translation="MKLLFPNGEHDAVEIGQRDVLVGAGADCAVMLAAPGVGPQHCKL
                     FERDGQTFVQPLGNAVTVLNGKQIAAEAPLKPGDLLLFARVGARVVAVEKSQAATLPR
                     RPLPAGDEDGRTRVRMALPKFVMRGVSGPTFGKTFGVVGTLTLGRSAECDISIPSDEI
                     SRHHAKLQVVPDGVMVEDMGSANGTFVNNQRVHGSTLMRHGDELRLDTV"
BASE COUNT          190 a          355 c          390 g          191 t
ORIGIN      
        1 gccgccgcct acgcgcagtg gctgggcaag caaaccggca agcgttaccg gctggcgagc
       61 ccggcagaat tcgatcgcgt tgcgcgccag gctgcgcgcg gcgattgcaa ggcgaacctg
      121 gccgatgccg cgtacaaacg caagttcgac agccgcgacg gcagcaattg cgacgacggt
      181 ttcgcggcca cctcgccggt gggcaagttt ccggctgcgg atggcgtgta cgacatcgat
      241 ggcaacgtgc gcgaatgggt agccgcgtgc agcggcggcg cgcccgcgca ggcgggcagc
      301 gattgccgtt cgttcatggt caagggccgt ggctggctgt cgcaggcggc cagggaatcg
      361 cccaccttca gcgataccta ttcgggcgac gtggcgctca acagcgtggg ttttcgcgtt
      421 gtgcgcgacg tcgacaaata aaaccggatt cgcgtttata ctggcgcgcg ttcgccactt
      481 tccaatgagg ttcgccggat gaagttgctg tttcccaacg gagagcatga cgcggtcgaa
      541 atcggccagc gcgatgtcct ggtcggcgcc ggcgccgact gcgcggtgat gctggccgcg
      601 cctggagtcg gccctcagca ctgcaagctt ttcgaacgcg atggccagac tttcgtgcag
      661 cctttgggta acgccgtgac cgtactcaac ggcaagcaaa tcgccgccga agcgccgctc
      721 aagcccggcg accttttgtt gttcgcgcgc gtcggcgcgc gcgtggtcgc ggtggaaaag
      781 tcgcaggccg cgacgttgcc gcggcgtccg cttccggcgg gcgacgagga cggacgcacg
      841 cgcgtgagga tggcactgcc gaaattcgtg atgcgcggcg tgtccggccc caccttcggc
      901 aagaccttcg gcgtggtcgg cacgctcacc ctggggcgca gcgccgagtg cgacataagc
      961 attcccagcg acgaaatctc gcgccatcac gcgaagctgc aggtcgtgcc cgatggcgtg
     1021 atggtcgagg acatgggttc ggcgaatggc accttcgtca acaaccagcg cgtgcatggc
     1081 tccacgctca tgcgccatgg cgacgagctg cgcctggata cggtgc
//
LOCUS       DASRVO010000486         1197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_14150, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000486 DASRVO010000000
VERSION     DASRVO010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14150"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1197)
                     /locus_tag="VFF05_06050"
     CDS             complement(<1..>1197)
                     /locus_tag="VFF05_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZW51401.1"
                     /translation="AEKLEGKLPHFAEAVSAETGATGMWAGFNVMLAAGMIREAAALT
                     TQVSGEVIPSDVPGSLSMGLRQPAGVVLGIAPWNAPIILGVRAIATPLACGNTVVLKG
                     SENCPRTHQLIIEAFQEAGFPPGVVNYITNAPADAGAVVEAMVSHPAVRRVNFTGSTK
                     VGRIIAQTCARHLKPVVLELGGKAPMVILDDADIDDAVNGTAFGCFANSGQVCMSTER
                     IIVDEKIADEFVRKFVAKAGALPVGDPRKGEPVVLGSVIGMGTVDHCNTLIDDALAQG
                     AKLLCGGKAQDTLMPATVLDHVTPKMRIYREETFGPVKCVVRVEGTEEAIACANDNEY
                     GLSAAVFGRDIARAFNVARRIESGICHVNAPTVHDEAQMPFGGVKGSGMGRFGGKAGI
                     AEFTDLR"
BASE COUNT          178 a          432 c          384 g          203 t
ORIGIN      
        1 gcgcaggtcg gtgaattcgg cgatgcccgc cttgccgccg aagcgcccca tgccgctgcc
       61 cttcacgccg ccgaacggca tctgcgcctc gtcgtgcacc gtcggcgcat tgacgtggca
      121 gatccccgac tcgatgcggc gcgcgacgtt gaacgctcgc gcgatatcgc ggccgaacac
      181 cgccgccgac aagccgtatt cgttgtcgtt ggcgcaggcg atcgcttcct cggtgccttc
      241 gacgcgcacg acgcatttca cgggaccgaa cgtttcctcg cgatagatgc gcatcttggg
      301 cgtcacgtga tcgagcaccg tggcgggcat cagcgtgtcc tgcgccttgc cgccgcacag
      361 cagcttcgcg ccttgcgcga gcgcgtcgtc gatcagcgtg ttgcagtggt ccaccgtgcc
      421 catgccgatc acgctgccca gcaccaccgg ctcgcccttg cgcggatcgc ccacgggcaa
      481 cgcgccggcc ttggcgacga acttgcgcac gaattcgtcc gcgatctttt cgtcgacgat
      541 gatgcgctcg gtggacatgc acacctgccc gctgttggcg aagcagccga aggccgtgcc
      601 gttcacggcg tcgtcgatat cggcgtcgtc gaggatcacc atcggcgcct tgccgccgag
      661 ctccagcacg acgggcttca ggtgcctcgc acaggtctgc gcgatgatgc ggcccacctt
      721 ggtggaaccg gtgaagttca cgcgccgcac cgccggatgc gacaccatcg cctcaacgac
      781 tgcgcccgca tcggccggtg cattggtgat gtagttgacg acgccgggcg gaaagcccgc
      841 ttcctggaac gcctcgatga tgagctggtg cgtgcgcggg cagttctccg agcctttgag
      901 gacgaccgtg ttgccgcacg cgagcggcgt cgcgatcgcg cgcacaccga ggatgatcgg
      961 cgcgttccac ggcgcgatgc ccagcaccac gccggccggc tgccgcaggc ccatggacag
     1021 cgagcccggc acgtcggacg ggatcacctc gccgctcact tgcgtggtca gcgccgccgc
     1081 ttcgcgaatc atgccggccg ccagcatgac gttgaagccg gcccacatgc cggtcgctcc
     1141 cgtctcggcg gacacggcct cggcgaaatg cggcaacttg ccctcgagct tctccgc
//
LOCUS       DASRVO010000487         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_222127, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000487 DASRVO010000000
VERSION     DASRVO010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222127"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFF05_06055"
     CDS             <1..528
                     /locus_tag="VFF05_06055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZW51402.1"
                     /translation="ERTLDQFLKSVERRALRMAELATGNRDEALDLVQDAMFGFARHY
                     AAKPAADWAPLFYRVLDSRLNDWHRRRSVRSRWLAVWTRHDDDDEHDEIAQAPDTNDP
                     GPLLRLAGSEAAHALDAALGNLPLRQRQAFLLRVWEGFDVAATASTMRCSEGSVKTHL
                     SRALAALRRALEDHA"
     gene            525..923
                     /locus_tag="VFF05_06060"
     CDS             525..923
                     /locus_tag="VFF05_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51403.1"
                     /translation="MNTNNLPNWTDRAKSLLDDSVRDLDAATLSRLNRARQAALAQRA
                     PRRRAAWLFVPAGLAGACALLLAVGVWHSQRPPTATLPTSTPAVAATTASKSLNTSDL
                     DMIASGDDLEMMQDLDFYAWLDAQDQDNNG"
     gene            923..1198
                     /locus_tag="VFF05_06065"
     CDS             923..1198
                     /locus_tag="VFF05_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51404.1"
                     /translation="MLPLLAAVTLALAAQPPAPQSTPPDAPVAKQGNKKMTRHDKSTQ
                     APDLDLIGYLGDYSDAADGLDPMGLADNAAAPKAAKIPPKSAPDAQP"
     gene            1195..1923
                     /locus_tag="VFF05_06070"
     CDS             1195..1923
                     /locus_tag="VFF05_06070"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HZW51405.1"
                     /translation="MKRRSLVVAVLVAALFACAGAWAQDQSAPPAAAQAAPIPWSSLS
                     SIEQQVLASAQDKWDTLSPAAQQRLQRGAQRWATLDPEQRADVRQRFQKWNALTPDQR
                     TKLRERYDQFKRLPPAQQERIRQAFKRFSSLPPAQRQVLREKFKQMNPAERRAFIAGV
                     QAQNQAGNVREFLLSIPKDQRQPTRQMLQALTPAERRAFRRTWHDLPENQRDAYRQNL
                     LHMTPQQRSAELAKPHPAAHSREH"
     gene            complement(1943..3142)
                     /locus_tag="VFF05_06075"
     CDS             complement(1943..3142)
                     /locus_tag="VFF05_06075"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HZW51406.1"
                     /translation="MDKSANPAEPATPVCLNCGAPLHGAYCHACGQPVKGMIRPLSGM
                     LHDVADTIFNIDSRVFHSLWPLYFRPGFLTREYFSGRRTRYVTPFRLYFFLSVLAFFT
                     IQFTLGESHLGKYMVNFDDADTPGISSAMTPQEVVARRDAQLSQLQSAKALTGAVIGA
                     TSAPGTVNKNVAAGMERAAAKIRKEADQRLAYLQKAADAKARGEPVPPDPNLETMSFN
                     GKPWDAQTNPLRISWLPEFANARLNAAIEHAKDNIPRIRKDPTLLIAGALGVLPQVLF
                     VLMPLFALLLKIFYIFKRRLYMEHLIVALHSHAFVFLSLFLFTLAGFAHAWTLTVAPG
                     LAPVLGLLLFVMGWWIPIYLFVMQKKVYGQGWFFTTVKFGAIGICYTIMISLGVAAAF
                     VISLATA"
     gene            3235..>3447
                     /locus_tag="VFF05_06080"
     CDS             3235..>3447
                     /locus_tag="VFF05_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZW51407.1"
                     /translation="MPISPTLPPRLRRELRETLRLAAPLVLGQLSAVGMNVVDTLLAG
                     HYSAQTLASVAIGTNVWMLAIVSAIGV"
BASE COUNT          638 a         1121 c         1135 g          553 t
ORIGIN      
        1 gagcgcacgc tcgatcagtt cctgaaaagc gtcgaacgcc gtgccctgcg catggccgag
       61 ctcgccacgg gcaatcgcga cgaggcgctc gacctggtgc aggacgcgat gttcggtttc
      121 gcgcgccatt atgccgccaa accggccgcc gactgggcgc cgctgtttta ccgcgtgctc
      181 gacagccgcc tcaacgactg gcatcggcgc cgcagcgtgc gcagccgctg gctcgcggtg
      241 tggacgcgcc acgacgatga cgacgaacac gacgagatcg cgcaggcgcc ggacacgaac
      301 gatcccggcc ccttgctgcg cctggcaggc agcgaagccg cgcacgcgct cgacgccgcg
      361 ctcggaaacc tgccgctgcg ccagcgccag gccttcctgc tgcgcgtgtg ggaaggtttc
      421 gatgtcgccg ccaccgcgag taccatgcgt tgcagcgagg gcagcgtgaa gacgcacctg
      481 tcgcgcgcgc tcgccgcgtt gcgtcgcgca ctggaggatc acgcatgaac acgaacaatc
      541 tgccgaactg gaccgaccgg gccaaaagcc tgctcgacga ctctgtgcgc gacctggatg
      601 cggccacgtt gtcgcgcctg aaccgcgcgc gccaggccgc gctggcgcag cgtgcgccgc
      661 gccgccgtgc ggcatggctg ttcgtgccgg cgggactggc cggcgcctgc gcactgctgc
      721 ttgccgtcgg agtgtggcat tcgcagcgtc cgccgaccgc gacgctgccg acatcgacgc
      781 ctgcggtcgc ggcgaccacg gcaagcaaaa gcttgaatac gagcgatctg gacatgatcg
      841 cgagcggaga cgacctggag atgatgcagg acctggattt ctacgcctgg ctcgatgcgc
      901 aggaccagga caacaacggc taatgcttcc gctcctggcc gctgtgactc tggcactggc
      961 ggcgcagccg ccggcgccgc aatccacgcc acccgatgcg cccgtggcga aacagggcaa
     1021 caagaagatg acgcgacacg acaaatccac gcaagcgcct gatctcgacc tgatcggcta
     1081 tctcggcgat tacagcgatg ccgccgacgg actcgatccc atgggcttgg ccgacaatgc
     1141 ggccgcgccg aaggcggcga aaattccgcc caagtcggca ccggatgcgc agccatgaag
     1201 cggcgctcgc tcgttgttgc cgtcctggtc gcggccttgt tcgcatgcgc tggtgcgtgg
     1261 gcgcaggatc aatccgcgcc accggccgcg gcgcaggcgg caccgattcc ctggtcgagc
     1321 ttgagttcga tcgaacagca ggtgctggcg tctgcgcagg acaagtggga cacgctttcc
     1381 ccggccgcgc aacagcgcct gcagcgcggc gcgcagcgtt gggcgacgct ggacccggaa
     1441 cagcgcgccg acgtgcgcca gcgcttccaa aagtggaacg cgttgacgcc cgaccagcgc
     1501 acgaaactgc gcgagcgcta cgaccagttc aagcgcctgc caccggcgca acaggagcgc
     1561 atccgccagg cgttcaagcg cttttccagc ctgccgccgg cgcagcgcca ggtgctgcgc
     1621 gaaaaattca agcagatgaa tccggccgaa cgtcgtgctt tcatcgctgg cgtgcaggcg
     1681 cagaatcaag cagggaacgt gcgcgaattt ctcctctcga ttcccaagga tcagcgccag
     1741 ccgacgcggc agatgctgca ggcgttgacg ccggccgagc gccgcgcgtt ccgccggacc
     1801 tggcacgacc tgcccgagaa tcagcgcgat gcgtaccggc agaatctgct gcacatgacg
     1861 ccgcagcaac gcagcgccga gctggccaaa ccgcatccgg cagcacattc gcgcgagcat
     1921 tgattgcggc ggttgggagc gatcacgccg tggccaggct gatcacgaat gcggcggcga
     1981 cgccgaggga aatcatgatc gtatagcaga tgccgatcgc gccgaatttc accgtcgtga
     2041 agaaccagcc ttggccgtag acctttttct gcatgacgaa caggtagatg ggaatccacc
     2101 atcccatcac gaacagcagt agacccagta cgggcgcgag gccgggcgcc acggtcagcg
     2161 tccacgcatg cgcgaatccg gcgagcgtga acaggaacaa cgaaagaaag acgaaggcgt
     2221 ggctgtgcag cgccacgatc aggtgttcca tgtacaggcg gcgcttgaaa atgtagaaaa
     2281 tcttcagcag cagtgcgaac agcggcatca gcacgaacag cacctgcggc agcacgccga
     2341 gcgcgccggc gatgagcagg gtcgggtcct tgcggatgcg cgggatgttg tccttggcgt
     2401 gttcgattgc cgcgttcagg cgcgcgttcg cgaactccgg cagccaggaa atgcgcaatg
     2461 gattggtttg cgcatcccac ggcttgccgt taaagctcat ggtttccagg ttcggatccg
     2521 gcggcaccgg ctcgcccctg gccttggcgt cggcggcctt ttgcagatac gcaagacgtt
     2581 gatcggcctc cttgcggatc ttggcggcgg ccctttccat tccggccgcg acgttcttgt
     2641 ttacggttcc cggcgccgat gttgcgccga tgaccgcgcc agtaagcgcc ttggcggatt
     2701 gcagctggga cagttgcgcg tcgcgccgtg ccacgacttc ctgcggcgtc attgcggacg
     2761 aaatgcccgg cgtatcggca tcgtcgaagt tgaccatgta cttgcccaga tgcgattcgc
     2821 cgagcgtgaa ctggatcgta aaaaacgcga gcacgctcag gaaaaagtac agacggaacg
     2881 gcgtcacgta acgcgtgcgc cgaccggaga aatactcgcg ggtcaggaag ccgggccgga
     2941 aatacagcgg ccacaggctg tggaaaacgc gcgaatcgat attgaagatc gtgtcggcaa
     3001 cgtcgtgcaa catgcccgat agcggccgga tcatgccctt taccggctgg ccgcaggcgt
     3061 ggcaataggc accgtgcagc ggcgcgccgc aattcaggca aacgggcgtt gcaggttcgg
     3121 cggggttcgc gcttttgtcc atcgcgcaca ctttagccga ttgcatgcgc agcggcgcga
     3181 tgacggcgcg cgatgcttct gcgggtacac tgcgcgatcc acgagcgcgc acccatgccg
     3241 atttctccaa ccttgccgcc gcgcctgcgc cgcgaattgc gcgagacgtt gcgattggcc
     3301 gcgccgctgg tgctcggcca gttgtcggcg gtcggcatga acgtcgtcga taccttgctg
     3361 gccgggcact acagcgcgca aacgctggcg tcggtcgcga tcggcactaa cgtgtggatg
     3421 cttgcgatcg tgtctgcgat cggcgtg
//
LOCUS       DASRVO010000488         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_326122, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000488 DASRVO010000000
VERSION     DASRVO010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326122"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VFF05_06085"
     CDS             complement(<1..1031)
                     /locus_tag="VFF05_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZW51408.1"
                     /translation="MTAATTATTPGGFARLLAWRRLKVTLIACTIIGLLLMPGWTASY
                     AVLFGRLYFIGTCLVLAFGLFERWPAQLPAWLARWALQVVGVAFFVPIAVWTAYTLTT
                     LGDATVWWHDRDRLIGFSSMTAMGLLLAPWIAMAALYRQVSGAAQRQALGFELERSEF
                     ERKALDARLRLLQAQVEPHFLFNTLANVRELVDSGSPQASAVLGHLIAYLRAAVPRLH
                     ETATTLGQEIELVRAYLEVMHMRMPDRLQFVLHADAAALALFCPPMTLLTLVENAVRH
                     GIDPSEEGGRIDVDVSRRGVRCAISVADSGVGLQQAGAGLGTGLSTLRERLALFFGSE
                     ATLRLSALEP"
BASE COUNT          198 a          385 c          357 g          150 t
ORIGIN      
        1 ggttcgagcg cgctcaggcg cagcgtcgct tcgctgccga agaacaatgc caggcgttcg
       61 cgcagcgtcg acaggccggt gccgaggccg gcgccggcct gctgcaagcc gacgccgctg
      121 tcggcgacgc tgatcgcgca gcgcacgccg cgccggctca cgtcgacgtc gatgcggccg
      181 ccttcctcgc tcgggtcgat gccgtggcgc acggcgttct cgaccagcgt cagcagcgtc
      241 atcggcgggc agaacagcgc cagcgccgca gcgtcggcgt gcagcacgaa ttgcagccgg
      301 tccggcatgc gcatgtgcat gacttccagg taggcgcgga ccagttcgat ctcctggccg
      361 agcgtagtcg ccgtctcgtg caggcgcggc actgcagcgc gcagataggc gatcagatgg
      421 ccgagcacgg ccgacgcttg cggcgaaccg gaatcgacca gttcacgcac gttggccagc
      481 gtattgaaca ggaaatgcgg ctcgacctgg gcctgcagca gccgcaggcg cgcatcgagc
      541 gccttgcgct cgaattcgct gcgctcgagt tcgaaaccca gcgcctgccg ttgtgccgcg
      601 ccgctgacct ggcgatacag cgccgccatc gcgatccatg gcgccagcag caggcccatc
      661 gccgtcatcg aggaaaaccc gatcaggcgg tccctgtcgt gccaccacac cgtcgcgtcg
      721 ccgagcgtcg tcagcgtgta ggcggtccac accgcaatcg gcacgaagaa ggcaacgccg
      781 accacctgca gcgcccagcg cgccagccat gccggcagct gcgccggcca gcgctcgaac
      841 aggccgaacg ccagcaccag gcacgtgccg atgaagtaca ggcgtccgaa cagcaccgca
      901 tacgaggccg tccatcccgg catcagcagc aggccgatga tcgtgcatgc gatcagcgtg
      961 accttcagtc gccgccaggc aagcagacgc gcgaagcctc cgggtgtcgt tgcagtcgtc
     1021 gcggcagtca tgacataacg atagcaacga acgcctcggt gaggtggatt cccgcaacct
     1081 gcagccgata
//
LOCUS       DASRVO010000489         1130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_91472, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000489 DASRVO010000000
VERSION     DASRVO010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91472"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFF05_06090"
     CDS             <1..519
                     /locus_tag="VFF05_06090"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW51409.1"
                     /translation="LRVPFLVKVFPQARFVFLHRDVRETLSSMIEAWRSGRFVTYRNL
                     PGWTGELPWSLLLTPGWRELNGKSLPGIVARQWETATRILLDDLAAIAPERITIARYD
                     SLLANPMAEIAHLCKALDFGWDRPLDKLPLSRYTVTPPDPDKWRRHEAEILGVLPEIQ
                     GTVDRAQDFAAR"
     gene            complement(516..>1130)
                     /locus_tag="VFF05_06095"
     CDS             complement(516..>1130)
                     /locus_tag="VFF05_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HZW51410.1"
                     /translation="LIGACARLPIAVDVTRLRAEVDALPESLWETRGGRLGPHDAARA
                     VFLRGYAPAEGRGKPVEDRDSLAHLPYAREIITQLLPAPPQRCLLARLPASAIVPPHS
                     DLGPYFLKTIRIHVPVITHENVWMYCDGCVFRMQAGEVWALNNVARHAVWNADATRAR
                     THLICDYLPSPALLDLLARAERDLGAVDADVEKRLLAESTTSMA"
BASE COUNT          178 a          368 c          381 g          203 t
ORIGIN      
        1 ctgcgcgtgc cgttcctggt caaggtattt ccgcaggcgc gattcgtgtt tttgcatcgc
       61 gacgtgcgtg aaacgctgag cagcatgatc gaagcctggc gctcggggcg tttcgtgact
      121 taccgcaacc tgccaggctg gaccggcgaa ctgccgtggt cgctgctgtt gacgccgggt
      181 tggcgcgagc tgaacggcaa atcgttgccc gggatcgtcg ctcgccagtg ggagacggcc
      241 acgcgcatcc tgctcgacga tcttgccgcg attgcgccgg aacgcatcac cattgcccgt
      301 tacgattccc tgctggcgaa cccgatggcg gagatcgccc atttgtgcaa ggcgctggat
      361 ttcggctggg atcgccccct cgacaagctg ccgttgtcgc gctacaccgt aacgccgccg
      421 gacccggaca agtggcggcg ccatgaggcg gaaatcctgg gcgtgttgcc ggaaatccag
      481 ggcactgtgg atcgcgccca ggacttcgcg gcgcgttagg ccattgatgt cgtcgattcg
      541 gcgagcagcc gcttctccac gtctgcgtcg accgcgccga gatcgcgctc ggcgcgcgcg
      601 agcaggtcga gcagtgcggg gctgggcaga tagtcgcaga tcaggtgcgt gcgcgctcgc
      661 gtcgcgtcgg cgttccacac cgcgtgcctg gcgacgttgt tgagcgccca cacttcgccc
      721 gcctgcatcc ggaatacgca tccgtcgcaa tacatccaga cgttctcatg cgtgatcacc
      781 ggtacgtgga tgcggatcgt tttcaggaaa tacgggccga gatcgctgtg cggcggcacg
      841 atcgcgctcg caggcaggcg cgccagcagg cagcgttgcg gcggcgccgg cagcagttgc
      901 gtgatgattt cgcgcgcgta cggcaggtgc gccagcgaat cacggtcttc gaccggtttg
      961 ccgcgccctt ctgccggtgc gtaaccacgc aggaagaccg cacgcgccgc gtcgtgcggg
     1021 ccgagccggc caccgcgcgt ttcccacagc gattccggca gggcgtcgac ttccgcgcgc
     1081 aaacgcgtca catcgacggc aatgggcaat cgcgcacagg cgccgatcag
//
LOCUS       DASRVO010000490         2843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_91481, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000490 DASRVO010000000
VERSION     DASRVO010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91481"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /gene="sucA"
                     /locus_tag="VFF05_06100"
     CDS             complement(<1..917)
                     /gene="sucA"
                     /locus_tag="VFF05_06100"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435566.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZW51411.1"
                     /translation="MSQSLLQQFAASSQLSGSNAGFIESLYETWLRDPDSIAPEWRNY
                     FDTFKGRQAGDVPHSDAIARIEAAQQRNGHAVAFATAPASDAHAQKQAAVLKLVTAYR
                     SRGHLAADLDPLGMAQKPPAPDLDPTFHGLAAADMETEFATGALAGPQKLKLKDLIAR
                     LKATYATSIGAEFMHISHTEQRRWVHERLEAAAGQFGLSADERRHTLELLTQAEGLER
                     YLHTKYVGQKRFSLEGGDSLIPLLDEIVRRGGEDGVRDMVIGMAHRGRLNVLVNTLGK
                     APQKLFAEFEGKFEHVDDPAHSGDVKYHMG"
     gene            complement(1017..1979)
                     /locus_tag="VFF05_06105"
     CDS             complement(1017..1979)
                     /locus_tag="VFF05_06105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZW51412.1"
                     /translation="MIPTDFSVAPVDWNNEAERDACRAVREQVFIVGQNVPREEEWDD
                     LDATSRHVLARDDAGNPIGTGRLTPERKIGRMAVLADWRGRHVGEAILNLLIEQARAL
                     GYPELEMHAQTHAVPFYEKFGFVKYGEEFEECAIKHFHMRRELAPLAVPPPRLISPRP
                     ERRVVAIASREDAAGETLRLIEAARHELCIYTRDLDPILLENEPALEALKRVAVSGRG
                     ASIRIIIQDPRTVVAHGHRLITLAQRLTSVFALRTPVEDNDLQYPSAFLLNDASGYYF
                     RTLATRFEGEAVNYAPGRHAQLLEYFNQVWERAELSEDLRQLSL"
     gene            complement(1991..>2843)
                     /locus_tag="VFF05_06110"
     CDS             complement(1991..>2843)
                     /locus_tag="VFF05_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZW51413.1"
                     /translation="LLDAFAFIPSWRVDDVMVSYATDDGGVGAHVDQYDVFLVQGMGR
                     RRWRISVDAHAPKDFRDDTELKLLREFTPTHDWVLEPGDALYLPPGVPHEGVAIGECM
                     TISVGMRAPSQAELLLDFAEFLAEPLGEERRFSDPDLRPARAPGEIDLAALARVRAAM
                     PQFSDVDISTLHDWFGRFITRYRSAHAALAASRPLAPARLAAKLAKSRLLRNPFSRFA
                     WIPVGRGAALFVAGERFDCPLALAKALCARHEFDGVALARMAGKSGAATLAALVDGGH
                     LRLLRGR"
BASE COUNT          489 a          896 c          965 g          493 t
ORIGIN      
        1 cccatgtggt acttcacgtc gcccgagtgc gcgggatcgt cgacgtgctc gaacttgccc
       61 tcgaattcgg caaagagttt ttgcggcgcc ttgccaagcg tgttgaccag cacgttcagg
      121 cgtccgcgat gggccatgcc gatgaccatg tcgcgcacgc cgtcttcgcc gccacggcgc
      181 acgatttcgt cgagcagcgg tatcagactg tcgccgcctt ccagcgaaaa gcgcttctgg
      241 ccgacgtact tggtgtgaag atagcgctca agaccctcgg cttgagtcag caattccagc
      301 gtgtgcctgc gctcgtcggc ggacaggccg aactgtccgg ccgcggcttc gagacgctcg
      361 tgtacccagc gccgctgctc ggtgtggctg atgtgcatga actccgcgcc gatggacgtt
      421 gcgtaagtcg ctttcaggcg cgcgatcagg tccttgagct tgagcttctg cggaccggcc
      481 agagcgccgg tggcaaactc ggtttccatg tccgcggcgg cgaggccgtg gaaggtggga
      541 tcgagatccg gcgccggcgg cttctgcgcc atgccgagcg gatcgaggtc ggcggccagg
      601 tgtccgcgcg agcgatacgc cgtgaccagc ttgagcaccg ctgcctgttt ctgcgcatgc
      661 gcgtcggaag cgggcgcggt ggcgaaggcg acggcatggc cgttgcgctg ctgtgccgcc
      721 tcgatgcgcg cgatcgcatc ggaatgcggc acgtcgccgg cctggcggcc cttgaaggtg
      781 tcgaagtagt tgcgccattc cggggcgata gaatccgggt cgcgcagcca ggtttcgtac
      841 agggattcga tgaatccggc gttgctgccg gaaagctggg acgatgccgc gaactgctgg
      901 agaagactct gactcacgct ggcctgccga gaacggaaag cgtcatccgc gggcgcggac
      961 gcacgaaccg gtaattatac gcaaatcagg gcggatgttg cgctgcgcaa gagcgcttac
     1021 agactcaact gccgcaaatc ctcgctgagc tcggcgcgtt cccagacctg gttgaagtat
     1081 tccagcaact gggcgtgacg gccgggcgca tagttcaccg cctcgccctc gaaacgcgtg
     1141 gcgagcgtgc ggaagtaata gccggaagcg tcgttgagca ggaatgcgct cgggtactgc
     1201 aagtcgttgt cctcgactgg cgtgcgcagt gcgaacacac tggtcaaacg ctgcgcaagc
     1261 gtaatcaggc ggtggccgtg cgcgacgacg gtgcgcggat cctggatgat gatgcggata
     1321 ctcgcgccac ggccggaaac cgcgacgcgc ttgagcgctt ccaaggccgg ctcgttttcg
     1381 agcaggatcg ggtcgaggtc gcgggtatag atgcacagct cgtggcgcgc cgcttcgatg
     1441 aggcgcaggg tttcgccggc cgcgtcctcg cgcgatgcga tggcgacgac gcgccgttcg
     1501 ggccgtggcg agatcagccg cggtggcggc acggccagcg gcgccagttc acggcgcatg
     1561 tggaagtgct tgatcgcgca ctcctcgaac tcttcgccgt atttgacgaa gccgaatttt
     1621 tcgtagaacg gcactgcgtg cgtttgtgcg tgcatttcca gttcgggata gcccagcgcg
     1681 cgcgcctgtt cgatcagcag gttgaggatc gcctcgccga cgtggcgacc gcgccagtcg
     1741 gccagcacgg ccatgcggcc gattttacgc tccggcgtca ggcgaccggt accgatggga
     1801 ttgccggcat cgtcgcgcgc cagcacatgg cgcgaggtcg cgtcgaggtc gtcccattct
     1861 tcctcgcgcg gcacgttctg gccgacgatg aatacctgtt cgcgcactgc gcggcaggcg
     1921 tcgcgttcgg cttcattgtt ccagtcgacg ggggcaacgg aaaagtcggt ggggatcatg
     1981 gggcgattat tcagcgtccg cgcaaaagcc gcaaatgccc gccatcgacc agcgccgcga
     2041 gagtcgccgc cccggatttg ccggccatgc gagcgagagc gacaccgtcg aattcgtggc
     2101 gcgcacaaag cgcctttgcc agtgccagcg gacaatcgaa tcgctcgccg gcgacgaaca
     2161 gcgccgcgcc gcgacccacg ggtatccaag cgaagcgact gaacggattg cgcagcagcc
     2221 gcgacttcgc cagcttcgcc gcgagtcgcg cgggcgcgag cggacgcgat gccgccagtg
     2281 ccgcatgcgc gctgcggtag cgggtgatga agcgtccgaa ccagtcgtgc aacgtggaaa
     2341 tatccacatc ggaaaattgc ggcatcgcag cgcgcacgcg tgcaagcgcg gccaggtcga
     2401 tctcacccgg tgcacgcgcg gggcgcaaat ccggatcgga gaagcggcgt tcctcgccca
     2461 gcggctcggc caggaattcg gcgaagtcga gcaacagttc cgcctgcgag ggcgcgcgca
     2521 tgccaacgga gatcgtcatg cactcgccga tggcgacacc ttcgtgcggc acgcccggcg
     2581 gcaggtacag cgcgtcgccg ggttcgagaa cccagtcgtg cgtcggcgtg aattcgcgca
     2641 gcaacttcag ttcggtatcg tcgcgaaaat ccttcggcgc gtgcgcatcg acgctgatcc
     2701 gccagcgtcg ccggcccatg ccttgcacga ggaacacgtc gtattgatcc acatgcgcgc
     2761 cgacgccgcc gtcgtcggtc gcgtaactca ccatcacgtc gtccacgcgc cagctcggga
     2821 tgaacgcgaa tgcgtcaagc agt
//
LOCUS       DASRVO010000491         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_351228, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000491 DASRVO010000000
VERSION     DASRVO010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351228"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFF05_06115"
     CDS             complement(<1..477)
                     /locus_tag="VFF05_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZW51414.1"
                     /translation="MDARPRRYTAPSRDILDAIGDTPLLEIDGIFAKCEFLNPSGSIK
                     ARIAKYIIDRAEQAGLLKPGDTIVEATSGNTGNAFAMVAAVRGYRMIVVMPEGLSSER
                     VAISRAFGAEVLFCGTFHVNAALERARELGRQPGFFFPGQFESEWNVEENREWLGPE"
     gene            complement(486..701)
                     /locus_tag="VFF05_06120"
     CDS             complement(486..701)
                     /locus_tag="VFF05_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51415.1"
                     /translation="MIPASIPQPRTSPARRITATGAPAALASAANVGTTRLEHRIDQT
                     LWQSFPTNDPPAPTQLDAMHAQAETVR"
BASE COUNT          161 a          311 c          370 g          188 t
ORIGIN      
        1 ctcgggcccc agccactcgc ggttctcctc gacgttccat tcggactcaa attggccggg
       61 aaagaagaat cccggctggc ggccgagttc gcgcgcgcgc tccagcgcag cgttgacgtg
      121 gaacgtgccg cagaacagca cctcggcgcc gaatgcgcgc gaaatcgcca cgcgctcgct
      181 ggacaggccc tcgggcatca ccacgatcat gcgatagccc ctgaccgccg cgaccatcgc
      241 gaaggcgttg ccggtattgc cgctggtggc ttcgacgatg gtgtcgccgg gcttgagcag
      301 gccggcctgc tcggcgcggt cgatgatgta cttggcgatg cgcgccttga tcgagccgga
      361 aggattgagg aactcgcatt tggcgaagat gccatcgatc tcgagcagcg gcgtgtcgcc
      421 gatcgcatcg aggatgtcgc gcgacggcgc ggtgtagcgt ctgggtctgg cgtccatcgc
      481 gagtctcagc gtactgtctc ggcctgcgcg tgcatcgcgt cgagttgcgt gggtgccggc
      541 gggtcgttgg tggggaaact ctgccacagc gtctggtcga tgcgatgctc gaggcgggtc
      601 gtgccgacat ttgcggcgga cgcgagtgcg gcaggcgcgc cggttgcggt gattcgacgc
      661 gctggcgatg ttcgtggttg cgggatcgaa gcaggaatca tggcggtcgg ccctcgatgg
      721 gacaggagag accggggcgg cgcgaggcgc gcgtcggagt gggatcatga tacgcctgcc
      781 gcacgcgtct tcaaaggtcg gagtggttgc gatcgagcac gttgagcgcc tgcgcacttg
      841 acctggatca gggcgacgcg aacttcgcgg cgtagcatgg ccgcgtctgc agattcccgg
      901 agttcgccat gcaagattcg ctggttccag aagcaaacac ccatgtcttc gatgcgcgcg
      961 gtgtcgcacg ccgcttccgc cactcggcaa tcttcggcgc gctcggcgcg ttgcgcaacg
     1021 gcgagacgat
//
LOCUS       DASRVO010000492         1765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_274468, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000492 DASRVO010000000
VERSION     DASRVO010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274468"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFF05_06125"
     CDS             complement(<1..702)
                     /locus_tag="VFF05_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZW51416.1"
                     /translation="MDSSKTRAFVSALWDEQIVPQLVEYIRIPNKSPMFDAQWVEHGY
                     MDKAVAQFAAWAKAQPIAGMHLDVVRLPGRTPLIFIEIPAFGGSADAEDCVLLYGHLD
                     KQPEMTGWAAHLGPWTPVIEGDRLYGRGGADDGYAMFGSLAAIRALQEQGVPHARCVV
                     LIEACEESGSYDLPYYVDHLAERIGKPSLVVCLDSGCGNYDQLWLTTSLRGLSGGNLT
                     VNVLEEGVHSGDASGI"
     gene            802..1704
                     /locus_tag="VFF05_06130"
     CDS             802..1704
                     /locus_tag="VFF05_06130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZW51417.1"
                     /translation="MSAIRLSLVLIPIVASTLLHVLPLFVVALLKAALPFARARAIGN
                     RLLTAIAESWIGVNNAMLDRFTPTRVDVHLLDGVDLRRDGHYLVLANHQSWVDILVLQ
                     KVFNRRIPLLRFFLKRQLFSVPLLGLAWWALDFPFMGRYTPKQIARNPELAGRDIAAT
                     RRACEKFRALPVSIMNFAEGTRFTAAKKASQSSPFVHLLKPKSGGVAFVLSAMGEALH
                     AIVDVSIAYPAGAPSMRDLLTGRVPEIRVRVRQRSIPAEFTSGDYQGDREFRVRFQRW
                     INTLWQDKDADLGTLLDGRREPRA"
BASE COUNT          311 a          609 c          530 g          315 t
ORIGIN      
        1 gatgccggag gcgtcgcccg agtgcacgcc ttcttccagc acgttcacgg tgaggttgcc
       61 accggacaat ccgcgcagcg acgtcgtcag ccataactga tcgtagttgc cgcagcccga
      121 atccaggcac acgacgagtg aaggcttgcc gatgcgctcg gccagatgat ccacgtagta
      181 cggcaggtcg taactgcccg attcctcgca ggcttcgatc agcaccacgc agcgcgcatg
      241 cggcacgcct tgttcctgca acgcgcggat cgcggcgagc gaaccgaaca tcgcgtaacc
      301 gtcgtcggcg ccgccgcgac cgtacaagcg atcgccctcg atgaccggcg tccatggccc
      361 caggtgcgcg gcccaaccgg tcatctccgg ttgcttgtcc aagtgcccgt ataacagcac
      421 gcaatcctcc gcgtcggcgc tgccgccgaa tgcgggaatt tcgatgaaaa tgagcggcgt
      481 gcgacccggc agccgcacca cgtccagatg catgccggca atgggctggg ccttggccca
      541 ggccgcgaac tgggcaacgg ccttgtccat gtatccgtgc tcgacccatt gcgcgtcgaa
      601 catcggcgac ttgttcggaa tgcggatgta ctcgacgagc tgcggcacga tctgctcgtc
      661 ccacaaggcg gaaacgaacg cgcgggtttt gctggaatcc atacgacacc atgtttggca
      721 ggcgaacatg cattttaccg cgtcgcgatg ggccgaagcg aaccagcgtg cgcagcgcaa
      781 cctgttagac tcgtccaccc gatgtccgcc atccgcctca gcctggtgct gattcccatc
      841 gtggccagca cgcttttgca cgtgctgccg ctgttcgtcg tggcgctgct gaaagcggcc
      901 ctgcccttcg cgcgcgcgcg cgcgatcggc aaccgcctgc tcaccgcgat cgcggaaagc
      961 tggatcggcg tgaacaacgc gatgctcgat cgcttcacgc ccacgcgcgt cgacgtgcac
     1021 ctgctcgacg gcgtggacct gcgccgcgac ggccactatc tcgtgctcgc caatcaccaa
     1081 agctgggtgg atatcctcgt cctgcaaaag gtgttcaacc ggcgcattcc gctgctgcgt
     1141 ttcttcctca agcgccagct gttctcggtg ccattgcttg gactggcgtg gtgggcgctg
     1201 gatttcccgt tcatgggccg ctacacgccc aagcagattg cgcgcaatcc ggaactggcc
     1261 ggacgcgaca tcgccgccac gcgtcgcgcc tgcgaaaaat tccgcgcgct gccggtgtcg
     1321 atcatgaact tcgccgaagg cacgcgcttc accgcggcca agaaggcctc gcagtcctcg
     1381 cccttcgtgc acctgctcaa gcccaagtcc ggcggcgtgg ccttcgtgct cagcgccatg
     1441 ggcgaagcct tgcacgcgat cgtggatgtc agcatcgcct acccggcggg agcgccgtcg
     1501 atgcgcgact tgctcaccgg gagggttccc gaaatacgcg tgcgcgtgcg gcagcgctcc
     1561 attcccgccg aattcaccag cggcgactat cagggcgacc gcgaattccg cgtgcgcttc
     1621 cagcgctgga ttaacacact gtggcaggac aaggacgccg acctcggcac attgctggac
     1681 gggcgccgcg aacctcgcgc atgacgcgga tgtttcatgc gatcatccgt cgccgcaaaa
     1741 tcaagccgac gccatgatcc ttccc
//
LOCUS       DASRVO010000493         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_222342, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000493 DASRVO010000000
VERSION     DASRVO010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222342"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1064)
                     /locus_tag="VFF05_06135"
     CDS             complement(<1..>1064)
                     /locus_tag="VFF05_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014090390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZW51418.1"
                     /translation="LIAAPTGSGKTLAAFLAAIDELVRDGVAGDLADETRIVYVSPLK
                     ALSNDVRINLEAPLTGIRDKLFEMGLPDVDIRASVRTGDTPQSERDAMKRRPPHIVVT
                     TPESLYILLGSESGRAMLASTRSVIVDEIHALAPSKRGAHLALSLERLESLCGQPLTR
                     IGLSATQKPIAEVARFLTGAKDVCEIVDVGHTRERDLAIELPPSPLSAVMSGDVWTQV
                     YARLAELITQHRTTLVFVNTRRMAERVARHLSELLGKDAVTSHHGSLAREQRLAAEQR
                     LKRGELRALVATASLELGIDIGDVDLVCQIASPRSIATFLQRAGRANHAVDGMPKARL
                     FPTSRDDLVECAALLDGVRRG"
BASE COUNT          173 a          334 c          370 g          187 t
ORIGIN      
        1 ccacgccgca ccccgtcgag cagggctgcg cattcgacca ggtcgtcgcg cgaggtcggg
       61 aacaggcgcg ccttcggcat gccgtcgacc gcatgattgg cgcggccggc gcgttgcaga
      121 aacgtcgcga tcgaacgcgg cgaggcgatc tggcaaacca ggtccacgtc gccgatatcg
      181 atgccgagtt cgagcgaggc ggtggcgacc agcgcgcgca gttcgccgcg cttgagacgt
      241 tgctcggcgg ccaggcgctg ctcgcgtgcc agtgaaccat gatgcgaagt gacggcatcc
      301 ttgccgagca gttcggacag gtgccgcgcc acgcgttcgg ccatgcgccg agtgttgacg
      361 aatacgagcg tggtgcgatg ttgcgtgatc aattcggcga gtcgcgcata gacctgcgtc
      421 cacacgtcgc cggacatcac cgccgacagc ggcgagggcg gcagttcgat agccaggtcg
      481 cgctcgcgcg tatggccgac gtcgacgatt tcgcagacat ctttcgcgcc ggtgaggaat
      541 ctcgccactt ccgcgatggg tttttgcgtc gctgaaaggc caatccgcgt cagcggctgt
      601 ccgcacaagc tttcaaggcg ttccagcgac agcgccaagt gcgcaccgcg tttgcttggc
      661 gccagcgcgt ggatttcgtc gacgatcacg ctgcgcgttg aagcgagcat ggcgcggccc
      721 gattctgaac ctagcaggat gtacagcgat tccggcgtcg taaccacgat atgcggcggg
      781 cgtcgcttca tggcatcgcg ctcgctctgc ggcgtatcgc cggtgcgcac gctggcgcgg
      841 atatcaacgt cgggcaggcc catctcgaac agcttgtcgc ggatgccggt gagcggcgct
      901 tccagattga tgcgcacgtc gttcgacagc gccttgagcg gcgagacgta gacgatgcgg
      961 gtttcgtcgg cgaggtctcc ggccacgccg tcgcgcacga gttcgtcgat cgcggcgagg
     1021 aaggcggcca gggtttttcc cgagccggtc ggcgcggcga tcag
//
LOCUS       DASRVO010000494         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_300904, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000494 DASRVO010000000
VERSION     DASRVO010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300904"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2077)
                     /locus_tag="VFF05_06140"
     CDS             complement(<1..2077)
                     /locus_tag="VFF05_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family decaheme-associated outer
                     membrane protein"
                     /protein_id="HZW51419.1"
                     /translation="MNTRERRFAVSAISLAVRTAMLALVVVPIVPMAQEAPEEGDTRA
                     LTCPTNYVEIGALNVPTDSPKFGEYTGLDSSGLYALADFDVSGGDAWCQGSGTLHWNA
                     TGTNLGTTSRNIGASVTDQGNWNFGIDYDQLRHYTVNGDYQTPLTGARGDNVFLLSPS
                     FGVINTTTTTSSGVLTSSNKGTQTLTPTQLAAFHHDDVYNQRDNTGASAGYAFNKQWS
                     VKFDFKRLDESGAKLISAGTDAYKIADGFNYGGERILVLMNPTEYRNDTFNLALNWVG
                     KQAYASLAYYASLFHDDYNGVSFSNPYVSGGTGNAPNPAPGTSPGAAFPLDTMSTPPS
                     NQFHQINFTGGYIFDPATKLTGGFSYARSTQNSSFDGTYTTTPNTVPGLPVGSFDGRV
                     LIKHADARLTHQWGSAINLSAGFKYNERDNTSASNTYTWLDLGGEAIETVNIPMSNKR
                     RQFDAAADWRIDARQRLHLGYEYEHIDRWCGNALANNAQGELSTTNAGYYVFASCVQV
                     PKDTENKLVGTYRFQLSDKLAFNAGYTHADRSADVNPSFYNPMQGNNQGFENYGYLAF
                     FDASRRENLFKAGLTWQATDKLSIDLNGRRTKDSYYDSALGVQNGDSGSANLDVDYSL
                     SQNVSFGGYASWQKRSRDLLTASGRNAVAPLATLWANTLTDRDNAIGLNGRQKGLWKG
                     KFELSEDFSY"
BASE COUNT          367 a          665 c          746 g          486 t
ORIGIN      
        1 cgtagctgaa atcctcgctc agctcgaact tgcccttcca caagcctttc tggcggccat
       61 tcaatcctat cgcgttgtcg cggtcggtca gcgtattcgc ccacaatgtg gcgagcggag
      121 ccaccgcgtt gcggccggac gccgtgagca ggtcgcgtga acgcttctgc cagctcgcgt
      181 agccaccgaa ggacacgttt tgggaaaggc tgtaatcgac gtccaggttc gcgctgcccg
      241 aatcgccgtt ttgcacgccg agcgcggaat cgtaatagct gtccttcgtg cgacggccgt
      301 tcagatcgat gctgagcttg tcggtcgcct gccacgtgag ccctgccttg aacaagttct
      361 cgcgccgcga cgcatcgaag aatgccaggt acccgtagtt ctcgaagccc tggttgttcc
      421 cctgcatcgg attgtagaac gacggattca cgtccgcgct gcggtcggcg tgggtatagc
      481 cggcattgaa cgcgagcttg tcactcagct ggaaccggta ggtcccgacg agcttgttct
      541 cggtgtcttt cggcacctgc acgcaactgg caaacacgta gtagccggcg ttcgtagtcg
      601 acaattcgcc ttgtgcgttg ttggcgagtg cattgccgca ccagcgatcg atgtgttcgt
      661 attcgtagcc caggtgcaga cgctggcgcg catcgatgcg ccagtcggcg gcggcatcga
      721 attgccggcg cttgttgctc atcgggatgt tgacggtctc gatcgcttcg ccgccgaggt
      781 cgagccatgt ataggtgttc gaggccgagg tgttgtcgcg ctcgttgtac ttgaaccccg
      841 cgctcaggtt gatcgcgctg ccccactggt gcgtgagcct ggcatcggcg tgcttgatga
      901 gcacgcgtcc gtcgaacgaa ccgaccggta gtccgggcac ggtgttgggc gttgtcgtgt
      961 aggtgccgtc gaaactcgaa ttctgcgtgc tgcgcgcgta ggaaaatccg ccggtcagct
     1021 tcgttgccgg atcaaagatg tagccgccgg tgaaattgat ctggtggaac tggttgctcg
     1081 gcggcgtgct catcgtgtcc agcgggaaag ccgcgccggg cgatgtcccg ggtgccggat
     1141 tcggcgcatt gccagtaccg ccggacacgt acgggttgga gaacgacacg ccgttgtaat
     1201 cgtcatggaa cagcgaagcg tagtaggcga gactggcata ggcctgcttg cctacccagt
     1261 tcagagcgag attgaaggtg tcgttgcggt attcggtggg gttcatcagg accaggatgc
     1321 gttcgccgcc gtagttgaag ccgtcggcga tcttatacgc gtcggtgccc gcgctgatca
     1381 gtttcgcgcc ggattcgtcc aggcgcttga agtcgaactt gacgctccac tgcttgttga
     1441 acgcatagcc ggcgctggcg ccggtgttgt cgcgctgatt gtagacgtcg tcgtgatgga
     1501 aggcggcgag ttgcgtcggc gtcagggttt gcgtgccctt gttcgagctc gtcagcacgc
     1561 cgctcgaggt ggtcgtcgtg gtattgatca cgccgaagct cggcgacagc aggaacacgt
     1621 tgtcgccgcg cgcacccgtg agcggggtct ggtaatcgcc gttcacggtg tagtggcgca
     1681 gttggtcgta atcgatgccg aagttccagt tgccctggtc ggttacgctg gcgccgatat
     1741 tgcgcgaggt cgtgcccagg ttcgtgccgg tcgcgttcca atgcaacgtg ccgctgccct
     1801 ggcaccaggc gtctccgccg ctcacgtcga aatcggccag cgcgtagagg ccggaggaat
     1861 ccaggcccgt gtattcgccg aatttcggcg agtcggtcgg cacgttcagt gcgccgatct
     1921 cgacgtagtt cgtcggacag gtcaacgccc tcgtgtcacc ttcctcgggc gcttcctgcg
     1981 ccatcggcac aatcggcacg acaaccaggg ctagcattgc cgtgcgcaca gcgagggaga
     2041 tggcgctgac ggcgaaacgg cgttcacgcg tattcatggc aacctccggg ttagcgatgc
     2101 aggaatgcac cggcgggaga gttcgaaccg tgcaccatca catggcagtt catgcaggcg
     2161 cgtccgacgg cgttgctgtt tggacttccg cccggcagga cgccgccacc ttgcaggccc
     2221 gcgacggcat tgccgtaagg cgcctggctg ttgtgcgggc cgtc
//
LOCUS       DASRVO010000495         1619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_351436, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000495 DASRVO010000000
VERSION     DASRVO010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351436"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /gene="hisH"
                     /locus_tag="VFF05_06145"
     CDS             complement(<1..253)
                     /gene="hisH"
                     /locus_tag="VFF05_06145"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009603840.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZW51420.1"
                     /translation="MANVVLVDSGGSNIGSLRFALQRLGVDACLTDDAEAIRNADRVI
                     LPGVGAADRGMARLRERGLVDTVRGLIQPVLGVCLGMQLL"
     gene            complement(253..1320)
                     /gene="hisB"
                     /locus_tag="VFF05_06150"
     CDS             complement(253..1320)
                     /gene="hisB"
                     /locus_tag="VFF05_06150"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HZW51421.1"
                     /translation="MNARKLLFLDRDGTLIVEPPDQQIDSFDKFAMIDGVIAALQKCV
                     AAGYELVMVTNQDGLGTPEFPQAAFDGPQNLLLQILGSQGMRFREVLIDASLPADGRD
                     TRKPGTGLVRHYLADDGWSRAQSAMVGDRETDARFAANLGIRAFRLDTGEWDWAGIAH
                     SLCDAPRRARVERATKETRVVASVDLDRTAEPIVNAGIGFFDHMLEQIGKHGGFALEL
                     RCTGDTHVDEHHSVEDSALALGEALRVALGDKRGTARYGFTLPMDEARASAVLDLSGR
                     ACFLLDGAFPRDRIGGLPTELVPHFFRSLCDALGANLHLSVRGENAHHMVEACFKSVG
                     RALRMALRRDGVELPSTKGAL"
     gene            complement(1317..>1619)
                     /locus_tag="VFF05_06155"
     CDS             complement(1317..>1619)
                     /locus_tag="VFF05_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZW51422.1"
                     /translation="PAALVETDARIARIRDERERLARALLALPVVIAVFASAANFLCV
                     RFADAPDAYRALATAGIIVRDVGGYPGISGCLRITVGAAQENAALLGALGACEAAA"
BASE COUNT          290 a          556 c          519 g          254 t
ORIGIN      
        1 acagcagctg catgccgagg cacacgccaa gcaccggctg gatcaagccg cggaccgtgt
       61 cgacgagacc gcgttcacgc aggcgcgcca tgcccctgtc cgcggcgccg acgccgggaa
      121 ggatcacgcg atcggcgttg cggatcgctt cggcgtcgtc cgtcaggcag gcgtcgacac
      181 cgaggcgctg caacgcgaaa cgcagcgagc ctatgttcga tccccccgaa tccaccagga
      241 ccacgttcgc catcacagcg cacccttggt gctcggcaac tcgacgccgt cacgacgcaa
      301 tgccatgcgc aatgcgcgtc cgaccgactt gaagcacgcc tcgaccatgt gatgcgcgtt
      361 ttcgccacgc acgctcaggt gcaggttcgc gccgagcgcg tcgcacagcg agcggaaaaa
      421 atgcggcacg agttcggtcg gcaacccgcc gatgcggtcg cgcgggaatg ccccgtctaa
      481 gaggaagcac gcgcgcccgg acagatccag gaccgcgctg gcccgtgcct cgtccatcgg
      541 cagggtgaac ccgtaacgag ccgtgccgcg cttgtcgccg agcgcaaccc gcagcgcttc
      601 gccgagagcc agcgcgctgt cctcgacgct gtggtgttcg tcgacgtgcg tgtcgcccgt
      661 gcaacgcaac tccagcgcaa atccgccatg cttgccgatc tgttccagca tgtgatcgaa
      721 gaatccgatt cccgcattca cgatcggttc ggcagtgcgg tcgagatcga cgctcgccac
      781 gacgcgtgtt tccttcgtcg cacgctcgac gcgcgcacgc cgtggcgcat cgcacagcga
      841 atgtgcgatg cccgcccaat cccactcgcc ggtatccagc cggaaggcgc ggatgcccag
      901 gttggcggcg aatcgcgcgt cggtttcgcg gtcgccgacc atcgccgatt gcgcacggct
      961 ccagccgtcg tcggcgagat aatggcgcac caacccggta ccgggcttgc gcgtgtccct
     1021 gccgtcggcg ggcaagctcg catcgatgag cacttcgcga aagcgcatgc cctgcgaacc
     1081 caggatttgc agcagcaggt tttgcgggcc gtcgaacgcg gcttgcggga actccggcgt
     1141 gccgagtcca tcctggttcg tgaccatgac gagttcgtag cccgcggcca cgcatttttg
     1201 caaggccgcg atcacgccgt cgatcatcgc gaatttgtcg aaggagtcga tctgctggtc
     1261 gggcggctcg acgatcaggg tgccgtcgcg atcgaggaaa agcagcttgc gcgcattcat
     1321 gccgccgcct cgcatgcgcc gagcgcgccc agcagggccg cgttttcctg cgccgcgcca
     1381 accgtgatgc gcaagcagcc ggaaattccc gggtaaccac cgacatccct gacgatgatg
     1441 ccggccgtgg cgagggcgcg gtatgcatcg ggcgcatcag cgaagcgcac gcaaagaaaa
     1501 ttcgccgcgg atgcgaatac ggcgataacc acgggcagcg caagcaacgc gcgggcgaga
     1561 cgttcgcgtt cgtcgcgaat acgggcgatg cgtgcgtccg tttccaccaa agccgctgg
//
LOCUS       DASRVO010000496         1304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_41256, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000496 DASRVO010000000
VERSION     DASRVO010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41256"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..515
                     /locus_tag="VFF05_06160"
     CDS             309..515
                     /locus_tag="VFF05_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51423.1"
                     /translation="MKTMQSDAASAGVPAALDRNASLYPEHWLANIRQMLRDGQRNAA
                     IRSLDQFRKKYPDYHLPDDLRDLR"
     gene            complement(518..>1304)
                     /locus_tag="VFF05_06165"
     CDS             complement(518..>1304)
                     /locus_tag="VFF05_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51424.1"
                     /translation="PVRETLHLADRRRDGSLWFWRLESGDAKARTHALWLVLATTADN
                     GNRVTLTPYRPLDPVAADEMFDGKRKFHFDAAQWAELAPCAQTGAWKDAQFQAQTDAA
                     ACSALLPGLGAAAALLPLRITLDGEMLHTVTFADQARGADAQIDARRVRWFSGWAAIN
                     GGGPKARAGNQDWHMQKDMRLSSEGGRAELHWRDGAASGYSLQLERTTYAERKLRVLQ
                     LNVIEDSSGQVIDYVWTDPAASAIGFNLGWLQVGLNQESAAAQ"
BASE COUNT          235 a          465 c          390 g          214 t
ORIGIN      
        1 tgtggcaacg gccggcgagt cgacccgaag tcgaaagcac ggctgctcca accgcaccgc
       61 cgcctgccac accgcaaccg gtcgaaacga cgtcaagcat gcgcaaagcc gccgctcccg
      121 cagcgacagc ggcacccgta gcacaggccc cggccgcgga aaagcaaaaa atggaaaatg
      181 ccgcatcgcg acctgcaccg cgcgccttcc ccgcgcccgc tcaggcgttg cgagaatcca
      241 ctccgccccc gccgccagct actgccgaag cgaagcctgc tttgtccgcg ccaacgatgg
      301 ctgcaccgat gaaaacgatg caatccgacg ccgcatccgc gggcgtgcca gcggcactgg
      361 atcgcaatgc ttcgctatat cccgagcatt ggctggcgaa tatccgccag atgctgcgcg
      421 acggtcagcg caacgcggcg atacgcagtc tcgatcaatt ccgcaagaaa tatccggatt
      481 atcacctgcc ggacgatctg cgcgatttgc gctgaggcta ttgcgcggct gcggattcct
      541 gattcagccc gacttgcagc cagcccagat tgaacccgat cgcgctggct gccggatccg
      601 tccacacata gtcgatgacc tgcccgctgg aatcctcgat cacgttgagt tgcaacacgc
      661 gcagtttgcg ctcggcgtag gtggtgcgtt cgagctgcag cgagtagccg ctagcggcgc
      721 catctcgcca gtgcagttcc gcgcggccgc cctcgctgga cagacgcatg tccttttgca
      781 tgtgccaatc ctgatttccg gcgcgtgcct tgggtccgcc gccgttgatc gccgcccagc
      841 cggaaaacca gcgcacgcgg cgcgcgtcga tctgtgcgtc tgcgccgcgc gcctgatcgg
      901 caaacgtgac ggtatgcagc atttcgccgt cgagcgtgat gcgcaacggc agcaaggcgg
      961 ccgcggcacc caatcccggc aacagcgcgc tgcacgccgc tgcatcggtc tgcgcctgga
     1021 attgcgcatc cttccacgcc cctgtttgcg cacacggcgc aagctccgcc cactgcgcgg
     1081 catcgaagtg aaatttgcgc ttgccgtcga acatctcgtc cgcggctacc ggatcgagcg
     1141 gacggtacgg cgtcagcgtc acgcgattgc cgttgtccgc cgtcgtcgcc aggaccagcc
     1201 acagcgcatg cgtgcgtgcc ttggcgtcgc ccgattcgag tcgccagaac cacaggctgc
     1261 catcgcgtcg gcggtcggcc agatgcaagg tctcgcgcac cggc
//
LOCUS       DASRVO010000497         1034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_118226, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000497 DASRVO010000000
VERSION     DASRVO010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118226"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1034)
                     /locus_tag="VFF05_06170"
     CDS             complement(<1..>1034)
                     /locus_tag="VFF05_06170"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZW51425.1"
                     /translation="PETDPELCALACRAGAGDARFFSLDAIPSPMAVPGRRIIVPLWA
                     IESLDWRQLRAMLAHELAHLARRDPQWKIAICAWRALFWFMPFAGIAQRRLDEIAELT
                     CDAWAARQTGDAHSVAQCLAACAEYRSAVPMPSFAPAMAVHRSLLMRRVDTLLDGDAV
                     RGDKSGLIARCLAATTLLAGMYALPAVTLIGASSVLADQAEPSPPSPPAPPSPSTPPT
                     AAGGHGSSHVSEVDILGWHTTSLSVSGPTRSLRAEIHGQIAFTPDETDVASLDAGGTA
                     MFQETVGGSTRRIELSEDKGQLKRHYFVNGSERAYDADAHTWFAALVPSLIRESGLGA
                     EARVARLYDK"
BASE COUNT          171 a          325 c          377 g          161 t
ORIGIN      
        1 tttgtcgtac agacgcgcta cacgcgcctc ggcgccgagg ccggattcgc gtatcagact
       61 cggcaccaaa gcggcgaacc aggtgtgggc gtccgcatcg taggcacgct cgcttccgtt
      121 tacgaaatag tggcgcttga gctggccctt gtcttcgctc aattcgatgc gacgggtgct
      181 gccgccgacg gtttcttgga acatcgccgt gccgccggca tcgagcgagg cgacgtcggt
      241 ttcgtccgga gtgaaagcga tctggccatg gatttcggcc ctgagcgaac gcgtgggacc
      301 ggacaccgac agcgacgtgg tgtgccagcc gaggatatcg acctcgctga cgtggctcga
      361 gccgtgacca cccgccgcgg tgggtggtgt ggacggcgaa ggcggtgccg gagggctggg
      421 cggtgacggc tcggcttggt cggcaagcac cgaactcgca ccgatcaggg tgacggccgg
      481 cagtgcgtac atgccggcga ggagagtcgt cgcggcgagg caacgcgcaa tgagaccgga
      541 tttgtcgcca cgcaccgcgt cgccgtcgag cagcgtgtca acgcgccgca tcagcagcga
      601 acggtggacg gccatcgcgg gtgcgaaaga cggcatcggc acggcgctgc ggtattcggc
      661 gcaggcggcc aggcattgcg cgacgctgtg cgcatcgccg gtttgccgcg cggcccaggc
      721 gtcgcaggtg agttcggcga tttcgtccag tcgccgctgg gcgatgccgg cgaagggcat
      781 gaaccagaac agcgcgcgcc aggcacagat tgcaatcttc cattgtggat cgcggcgcgc
      841 cagatgtgcc agttcgtgcg ccagcatcgc gcgcagttgg cgccagtcca gcgactcgat
      901 cgcccaaagc ggaacgatga tgcggcgccc cggtaccgcc atcgggctgg ggatggcatc
      961 gagggagaaa aagcgcgcgt cgccggcgcc cgcgcggcac gcgagcgcgc acagttccgg
     1021 gtccgtctcc ggcc
//
LOCUS       DASRVO010000498         1262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_300952, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000498 DASRVO010000000
VERSION     DASRVO010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300952"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VFF05_06175"
     CDS             complement(<1..290)
                     /locus_tag="VFF05_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZW51426.1"
                     /translation="MELAQEFGVCVVGPRGAGEFLPAQIRVDEVRAAPLWKFFGGALW
                     RSVRAASRGGRPDIVLGGSGLVAPFVWLAGRIANARVALYVHGLDLIAPHFV"
     gene            complement(388..1098)
                     /locus_tag="VFF05_06180"
     CDS             complement(388..1098)
                     /locus_tag="VFF05_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZW51427.1"
                     /translation="MSSDDLNAIRDFYDNRYYGQHAHGARPAWHTRKIAARLGDPAGW
                     QVLDVACGTGGWLNLFRKRGAGIAGIDLSQMAIEVCRRELPDGEFVCGPAETLPFADA
                     RFDLVTCMGSLEHFVDKPAALAQMRRVAKPDARFLILVPNAGFLTRRLGLYGGTQQVM
                     AKEDVYELAEWNRLFEAAGLRVIERWRDLHPLSWSWIARGSRLGWPVRAAQALALAAW
                     PLGWQYQVYHYCRACDVA"
     gene            complement(1113..>1262)
                     /locus_tag="VFF05_06185"
     CDS             complement(1113..>1262)
                     /locus_tag="VFF05_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017910391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW51428.1"
                     /translation="TGLGYYLYTFFHMHRLTNMSVLLFSASVIVFLIGLISEQITALT
                     YRRDA"
BASE COUNT          264 a          421 c          385 g          192 t
ORIGIN      
        1 tagacgaagt gtggcgcgat caggtccaac ccatgcacgt acagcgcgac gcgcgcattg
       61 gcgatccttc ccgcgagcca gacgaagggc gcgacaaggc cgctcccacc cagcacgatg
      121 tcgggacgtc cgccgcgcga agccgcgcgt acgctgcgcc acaacgcgcc accgaaaaat
      181 ttccacagcg gcgcggcacg gacttcatcc acgcgaattt gcgccggcag gaattcaccc
      241 gcgccacgcg ggccgaccac gcacacgccg aactcttgcg cgagttccat cgccatatgc
      301 cgattcagcc gttccatccc tccgcgtaat ggcggcaggt tgcgcgtgac gagcagtaca
      361 cggagcggtc gggaccccgt ggccggctca agcgacatcg caggcacggc aatagtggta
      421 cacttggtac tgccagccta gcggccatgc cgccagtgcc aatgcctgcg cagcgcgaac
      481 cggccagccc agcctgctgc cgcgggcgat ccacgaccaa gacaacggat gcaggtcgcg
      541 ccagcgttca atcacgcgca ggccggcagc ctcgaacaga cgattccatt cggcgagttc
      601 gtagacatcc tccttcgcca tgacctgctg cgtgccgcca taaaggccca gacggcgcgt
      661 caggaatccg gcgttcggga ccaggatcaa aaatcgcgca tccggtttgg cgacgcgtcg
      721 catttgcgcc agcgcggcgg gtttgtcgac gaaatgttcc agcgatccca tgcaggtgac
      781 gagatcgaaa cgcgcatcgg cgaacggcag cgtttcggca gggccgcaga cgaattcgcc
      841 gtccggcagt tcccggcggc aaacctcgat tgccatctgc gacagatcga tgccggcgat
      901 gcccgcaccg cgtttgcgga acagattgag ccagccgccg gtgccgcaag ccacatcgag
      961 tacctgccag ccggccggat cgcccagccg cgcggcgatc ttgcgcgtat gccaggccgg
     1021 gcgcgcgcca tgcgcatgct gcccgtaata ccgattgtcg tagaaatcgc ggatcgcatt
     1081 caggtcgtct gaggacattt caaacgcgcc cgtcaagcat cgcgccgata cgtcaacgcc
     1141 gtgatctgtt cggagatcag cccgatcagg aacacgatca cggatgcgga aaacagcagc
     1201 acgctcatgt tggtgagacg gtgcatgtgg aaaaaagtat acaaatagta gccgaggccg
     1261 gt
//
LOCUS       DASRVO010000499         1761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_41312, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000499 DASRVO010000000
VERSION     DASRVO010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41312"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..434
                     /gene="rplR"
                     /locus_tag="VFF05_06190"
     CDS             78..434
                     /gene="rplR"
                     /locus_tag="VFF05_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002811659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZW51429.1"
                     /translation="MSEKNIARLRRAKTTRAHIRKLGVPRLTVHRTDQHIYAQVITAE
                     GDKVLAAASTVQKDLAKGLKGTKNKTAAAAVGKAIALKAKAAGVESVAFDRSGFRYHG
                     RIAALADAAREAGLKF"
     gene            518..1036
                     /gene="rpsE"
                     /locus_tag="VFF05_06195"
     CDS             518..1036
                     /gene="rpsE"
                     /locus_tag="VFF05_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZW51430.1"
                     /translation="MANNNERENSDGMLEKLIAVNRVAKTVKGGRQMSFTALTVVGDG
                     DGRVGLGYGKAREVPVAISKAMERARRGMVSVKLNNGTLWHAVKANHGAARVYMQPAS
                     EGTGVIAGGAMRAVLEVVGVKNVLAKAVGSRNPINLVRATIKGLQLMATPERIAAKRG
                     KKVSEVGGYGQG"
     gene            1023..1232
                     /gene="rpmD"
                     /locus_tag="VFF05_06200"
     CDS             1023..1232
                     /gene="rpmD"
                     /locus_tag="VFF05_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZW51431.1"
                     /translation="MAKAKTTKAAGTVRVRLYKSMNSCQWKHRLSVKALGLNKLNDVR
                     ELKDSPQVRGLVEKINYLVRIEESA"
     gene            1263..1697
                     /gene="rplO"
                     /locus_tag="VFF05_06205"
     CDS             1263..1697
                     /gene="rplO"
                     /locus_tag="VFF05_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZW51432.1"
                     /translation="MRLNTLKPAKGSRKTRVRVGRGIGSGLGKTAGRGHKGQFARTGK
                     GKVKPGFEGGQMPLQRRLPKVGFRSKMARDVCEVLLYKLDSIKGDAIDFAALKAAGLV
                     DTRAIRAKVVKKGEIKRAVKLKGVAATAGAKAAILAAGGSVE"
BASE COUNT          390 a          541 c          581 g          249 t
ORIGIN      
        1 cgctatgccg gcgaacgcat caccctcaaa gaagccaaga aggcgtaacg ccgccaatcc
       61 gcttcaggag aacgaaaatg agcgaaaaaa acattgcccg actgcgccgc gccaagacca
      121 cgcgtgcgca cattcgcaag ctcggcgtgc cgcgcctgac cgtgcaccgc accgaccagc
      181 acatctacgc ccaggtcatc accgccgaag gcgacaaggt gctggccgcg gcctcgaccg
      241 tgcagaagga tcttgcaaag ggcctgaaag gcaccaagaa caagacggct gccgcggcgg
      301 ttggcaaggc gatcgcgctg aaggccaagg ccgccggcgt ggaatcggtg gcattcgacc
      361 gctccggttt ccgttatcac ggccgcatcg ccgcgctcgc ggacgccgcg cgcgaagcgg
      421 gcctgaaatt ctgattcaac gatccaacca caactacaag cgacctcggt cgtaatcgaa
      481 gtccaccgga taaccggcgg caaacaggcg agaacagatg gcaaacaaca acgaacgtga
      541 aaacagcgac ggcatgctgg aaaagctcat tgccgtcaac cgcgtggcga agacggtcaa
      601 gggcggtcgg cagatgagct tcaccgcgct gaccgtggtt ggcgacggcg atggccgggt
      661 cggcctcggc tacggcaagg ctcgcgaagt gccggtcgcg atctccaagg cgatggagcg
      721 tgcgcgccgc ggcatggtca gcgtcaagct caacaacggc acgttgtggc atgcggtcaa
      781 ggccaaccat ggcgccgccc gcgtctacat gcagccggct tccgaaggca ccggcgtgat
      841 cgccggcggc gcgatgcgcg ccgtgctcga ggtggtcggc gtcaagaacg tgctggccaa
      901 ggccgtgggt tcgcgcaatc cgatcaacct ggtgcgcgcc acgatcaagg gtctgcaatt
      961 gatggccacg ccggagcgta tcgcggccaa gcgcggcaag aaggtaagcg aggtgggtgg
     1021 ttatggccaa ggctaagaca acgaaagcag cgggcacggt ccgcgtgcgc ctctacaaga
     1081 gcatgaattc ctgccagtgg aagcatcgcc tgagcgtgaa ggcgctgggc ttgaacaagc
     1141 tcaacgacgt gcgcgagctg aaggattcgc cgcaggtacg cggcttggtc gaaaagatca
     1201 attacctcgt gcgcatcgaa gaaagcgcgt aagcagtaaa gcatatttct ggagttcgca
     1261 tcatgcgtct gaatacactc aagccggcca agggatcgcg caagacgcgc gtgcgcgtcg
     1321 gccgcggcat cggttcggga ctcggcaaaa ccgcgggtcg cggccacaag ggccagttcg
     1381 cccgcaccgg caagggcaag gtgaagcccg gcttcgaggg cggccagatg ccattgcagc
     1441 ggcgcctgcc gaaggtcggc ttccgctcca agatggcgcg cgatgtttgc gaagtgctgc
     1501 tgtacaagct cgattcgatc aagggcgacg ccatcgattt tgccgcgctc aaggcggccg
     1561 gtctcgtcga cacgcgcgcg atccgcgcca aggtcgtcaa gaagggcgag atcaagcgcg
     1621 cggtcaagct caagggcgtg gccgcgaccg cgggcgccaa ggcggcgatt ctcgctgccg
     1681 gcggcagcgt ggagtaaaag gtggccgcac cccaaaacaa tggcctgctc ggcaatctcg
     1741 gcaaactgac cgagctcaag c
//
LOCUS       DASRVO010000500         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_143687, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000500 DASRVO010000000
VERSION     DASRVO010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143687"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFF05_06210"
     CDS             complement(<1..183)
                     /locus_tag="VFF05_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51433.1"
                     /translation="MLWDNARSPERAVPLAMIVHDGLTALSPFRLERLNRDLAVAAPQ
                     SRVSAAWWVYFVQAEAN"
     gene            complement(207..959)
                     /locus_tag="VFF05_06215"
     CDS             complement(207..959)
                     /locus_tag="VFF05_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZW51434.1"
                     /translation="MLKKFLVLAAIAATAPFAHAADPAEKTVRDALHSMLPLAVIDKV
                     AKSDLPGFYEVVVSGQIAYVSADGKFLMQGSLYDVAHKRNLTDASLAAMRAQAIAKLP
                     ASKELRFDVANPKHTVTVFTDVDCPYCRAFHKNIGQFNALGISVHYVLFPLDIHPGAD
                     KKAVAVWCTQDRNKAYTDAMNGKDPGNATCTNPVSETKALGIALGINATPTVLTEDGT
                     SVDLARATSPQALLAELDRLAGERTKTKVAAR"
     gene            complement(1014..1742)
                     /locus_tag="VFF05_06220"
     CDS             complement(1014..1742)
                     /locus_tag="VFF05_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZW51435.1"
                     /translation="MVRFVLISLLAMFAVSAVAADDPTAVVTQALHKLAPNAKVESVA
                     PSPLAGFYAVVADGRPIFISTDGKYLIDGHVYDIAARRNVMDDAMAGVRKQALANIPQ
                     SKRITFAPPHPKYHVTVFTDVDCPYCREFHKQIAEYNQLGIAVDYVLFPLSIHPGADK
                     KAQTVWCSKDRNVAYNEAMDGKALSPLTCSNPIAEITNTANAIGINGTPAIFADDGAQ
                     LGGYVPPAQLAKQLQDLAQRKSAP"
     gene            complement(1817..2758)
                     /gene="xerD"
                     /locus_tag="VFF05_06225"
     CDS             complement(1817..2758)
                     /gene="xerD"
                     /locus_tag="VFF05_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZW51436.1"
                     /translation="MPAESTPPVNAIDAAAIDTFVEKLWSESGLADNTLSSYRLDLEG
                     LSRWLAGQGRTLDGATRALLNAYLASRFSAGGRRGTGFSARSNARLLSTLRHFYRQRA
                     ALGAIAEDPTLLLDAPKLPRPLPKALSEREVEALIRAPDVETPLGLRDRAMFELIYAT
                     GLRVSELVGLRTEQINLRQGVLRVTGKGGKERLVPLGEEAQDWLERYYAQARPVLLRG
                     AAVDAVFVTARRAGMTRQMFWTMVKNHARKAGIDAKRISPHVLRHCFATHLLNHGADL
                     RALQMLLGHSSLSTTQIYTLVAREGLKRLHERHHPRG"
BASE COUNT          479 a          912 c          997 g          533 t
ORIGIN      
        1 gttggcctcg gcttggacga aatagaccca ccacgcggcg ctcacgcggc tctgcggagc
       61 ggccacggcg aggtcgcggt tcagacgctc caggcgaaac ggggaaaggg ccgtgagccc
      121 gtcgtggacg atcatggcga gcggaacagc gcgttcggga gaacgcgcat tatcccacag
      181 caccgcgccg gagcgggcgg atcgaatcag cgcgcggcga ctttcgtctt ggtccgctca
      241 ccggccagcc gatcaagctc ggcgagcagc gcctgcggcg aggtcgcacg ggccaggtcc
      301 accgaggtgc cgtcttcggt gagcaccgtc ggcgtggcgt tgatgccgag cgcgattccc
      361 aacgccttgg tttcggacac cgggttggtg caggtcgcat tgcccggatc cttgccgttc
      421 atggcgtcgg tgtaggcctt gttgcggtct tgcgtgcacc acacggcgac ggccttcttg
      481 tcggcgcccg gatggatgtc gagcgggaac agcacgtaat gcacggaaat gccgagcgcg
      541 ttgaactgtc cgatgttctt gtggaaggcg cggcagtacg ggcagtccac atcggtgaac
      601 acggtgacgg tgtgcttggg attggccacg tcgaagcgaa gttccttcga cgccggtaat
      661 tttgcgatgg cctgcgcacg catggccgcg aggctcgcgt cggtcaggtt gcgcttgtgc
      721 gccacgtcgt acaggctgcc ctgcatgagg aacttgccgt cggcgctgac gtaggcgatc
      781 tggccgctga cgaccacttc gtagaatccc ggcaggtcgg atttggccac cttgtcgatc
      841 accgccagcg gcagcatcga gtgcaacgcg tcgcgcacgg ttttctcggc gggatcggcg
      901 gcatgcgcga aaggcgcggt ggcggcgatg gcggcaagga cgaggaattt tttcaacatg
      961 acacgttccg attggagtct ggacgcattg gacaatgccg atgcgcgctt ggttcagggc
     1021 gcggacttgc gctgcgccag atcctgcagt tgcttcgcca gttgcgccgg cggcacatag
     1081 ccgcccaact gcgcgccgtc gtcggcgaaa attgccggcg tgccgttgat gccgatggcg
     1141 ttcgcggtgt tggtgatttc ggcaatggga ttcgagcagg tcagcggcga aagcgccttg
     1201 ccatccatgg cctcgttgta ggccacgttg cggtccttcg aacaccacac ggtttgcgcc
     1261 ttcttgtccg cccccggatg gatgctgagc ggaaacagca cgtagtcgac ggcgatgccg
     1321 agctggttgt attcggcaat ctgcttgtgg aattcgcggc aatacgggca atcgacatcc
     1381 gtgaacaccg tgacgtggta cttcggatgc ggcggcgcga aagtgatgcg cttgctctgc
     1441 gggatattgg ccaatgcctg cttgcgtacg ccggccatgg catcgtccat gacgttgcgg
     1501 cgcgcggcga tgtcgtagac gtggccatcg atgaggtatt tgccgtccgt gctgatgaaa
     1561 atcgggcggc cgtcggcgac gaccgcgtag aacccggcca gcggcgaggg cgccacggac
     1621 tcgaccttcg cgttcggcgc gagcttgtgc agggcctggg tgacgactgc ggtcggatcg
     1681 tcggcagcta cggccgacac ggcgaacatc gccagaagtg aaatcaacac gaatcggacc
     1741 atgagaatct cgcgaaagcg cgaattgcgc attatcgacc ggcgcagcgg gtcggatatg
     1801 acaattatcg cacagctcat cctctgggat gatgccgctc gtgcagccgc ttcaaccctt
     1861 cgcgcgctac cagcgtgtag atctgcgtgg tcgacagcga gctgtgaccg agcagcatct
     1921 gcagggcgcg caggtcggca ccgtgattga gcaggtgcgt ggcgaagcaa tgccgcagca
     1981 cgtgcggcga gatgcgcttc gcgtcgatgc cggccttgcg cgcgtggttc ttcaccatcg
     2041 tccagaacat ctgccgggtc atgccggcgc ggcgcgccgt gacgaagacc gcgtcgaccg
     2101 cggcgccgcg cagcagcacg ggacgcgctt gcgcgtagta gcgctccagc cagtcctgtg
     2161 cctcctcgcc gagcgggacg aggcgctcct tgccgccctt gccggtcacg cgcagcacgc
     2221 cctggcgcag gttgatctgc tcggtgcgca ggcccacgag ttcgctgacg cgcaagccgg
     2281 ttgcatagat cagttcgaac atggcgcggt cgcgcaggcc cagcggcgtt tcgacatccg
     2341 gcgcgcggat cagcgcctcg acctcgcgct cggacagcgc cttgggcaag ggccgcggca
     2401 gtttcggcgc atccagcagc aaggtcggat cttcggcaat cgcgccgagc gccgcgcgtt
     2461 gccggtagaa atgccgcaac gtggacagca gccgcgcatt gctgcgcgcg ctgaatcccg
     2521 tgccgcgccg gccgccggcc gaaaaccgcg aggcgagata cgcgttcagc aatgcgcgcg
     2581 tggcgccgtc cagagtgcgt ccctgccccg ccagccagcg cgacaggcct tccagatcca
     2641 gccggtaact cgacaacgtg ttgtctgcca gtccgctttc cgaccagagc ttttcgacga
     2701 aggtgtcgat ggcggcggcg tcgatcgcgt tgacgggtgg tgtagattca gccggcatga
     2761 cgcatggagt ttccgatgaa cgtgcctgct tccgcaacac ccgctccact gcgattgcgt
     2821 ctggccgccg ccgcctacga cctgctgccg ctgatcggac tgtggttcgt cgctgcggtg
     2881 ctcgctctcg tcgtcaccgg cggtgcgctc gacacgcaca c
//
LOCUS       DASRVO010000501         1402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_300996, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000501 DASRVO010000000
VERSION     DASRVO010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300996"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFF05_06230"
     CDS             complement(<1..728)
                     /locus_tag="VFF05_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZW51437.1"
                     /translation="MSTATREAIPADVQALEQRLGAYLPQDQIARVRQAYQTGARAHE
                     GQKRQSGEAYITHPVAVAGILAEIGMDAETIIAAILHDTLEDTPLSRAEIEATFGVTV
                     AELVDGVTKLDKLRFRSVAEAAAESFRKMLLAMARDLRVILIKLADRLHNMRTLAAMA
                     PEKRRRIARETLDIYAPIAQRLGMNRTRAELQDLGFRALYPDRYRIIAARIKSVFGNR
                     REAMGKIEAALAARLEQEKIPHRVV"
     gene            complement(959..1249)
                     /gene="rpoZ"
                     /locus_tag="VFF05_06235"
     CDS             complement(959..1249)
                     /gene="rpoZ"
                     /locus_tag="VFF05_06235"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002812428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZW51438.1"
                     /translation="MARITVEDCLAVVDNRFELVLMATRRARQVAKGADALVETHNDK
                     PTVVALREIAGRKVTHEMIDEVDRVERERKEREALEWAAAEVDEDIKGGDDL"
BASE COUNT          236 a          437 c          451 g          278 t
ORIGIN      
        1 acgacgcggt gcgggatttt ttcctgctcc aggcgcgcgg ccagtgctgc ttcgatcttg
       61 cccatcgcct cgcggcggtt gccgaacacg ctcttgatgc gcgcggcgat gatgcggtag
      121 cgatcggggt acaaggcgcg gaaacccaga tcctgcaatt ccgcgcgcgt gcggttcatg
      181 cccaggcgct gcgcgatcgg cgcgtagatg tcgagcgtct cgcgcgcgat gcggcggcgt
      241 ttttccggcg ccatcgccgc cagcgtgcgc atgttgtgca ggcgatcggc gagcttgatc
      301 aggatgacgc gcagatcgcg cgccatcgcc agcagcatct tgcggaaact ctcggccgcg
      361 gcctcggcga cgctgcggaa acgcagcttg tcgagtttgg tgacgccatc gacgagttca
      421 gccacggtga cgccgaacgt ggcttcgatt tccgcgcgcg acagcggcgt gtcctcgagc
      481 gtgtcgtgca ggatcgccgc aatgatcgtt tcggcatcca tgccgatctc ggcgaggatg
      541 ccggcaaccg cgaccggatg cgtgatgtag gcctcgccgc tctggcgttt ctgcccttca
      601 tgggcgcggg caccagtctg gtaggcctga cgcacccgcg caatttgatc ctgcggcaaa
      661 taggcgccga ggcgttgctc cagcgcttgc acgtcggcag ggatggcttc ccgcgtcgcc
      721 gtgctcatga tcgtgccgcg tgggcgatca gaccgtccgc cggtggcagg cggtcaaaat
      781 gggacggccg gaagaaccac tcgtatcaag gtagccgcaa gccatgttca gaaccgtcgc
      841 gagcgaacgc agattgcagg ccgccggagc gcaggaagcg gagtgtactt tgacgtacat
      901 gagcatttcg agcaccgccg ggatgcaagc tgcggagcgc agcaggttct ggaatggctt
      961 aaaggtcgtc gccgcccttg atgtcttcat cgacctcggc agcagcccat tccagggctt
     1021 cgcgttcctt gcgctcgcgc tcgacgcggt cgacttcatc gatcatctcg tgcgtaacct
     1081 tgcgtcccgc gatttcgcgc agcgcgacca cggtcggttt gtcgttgtgc gtttccacga
     1141 gggcatcggc acccttggcg acctggcgtg cgcggcgggt ggccatcagc accagttcga
     1201 agcggttgtc gaccactgcc aggcaatctt ccacggtaat gcgtgccata aaatcctcaa
     1261 aatcgaaagc aattcatact attacacaga ctatattgta accgccgcgc atgctgcggt
     1321 gcaaacctgg catctgaacg gcaactggcc agcgtggccg tgttgccgtg caatatgccc
     1381 cgttcctgcg gtaggcttgc ct
//
LOCUS       DASRVO010000502         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_169240, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000502 DASRVO010000000
VERSION     DASRVO010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169240"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..424)
                     /locus_tag="VFF05_06240"
     CDS             complement(83..424)
                     /locus_tag="VFF05_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZW51439.1"
                     /translation="MSAQPSGKTFREVPASAVIAMRERGDDVTFVDVREQHEWNLFRI
                     GGAVHIPLAMVRDEAPGRLEADRTVILYCARGGRSATAAATLAELGYADVISLEGGLM
                     AWVNAGGPLEE"
     gene            628..1794
                     /locus_tag="VFF05_06245"
     CDS             628..1794
                     /locus_tag="VFF05_06245"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW51440.1"
                     /translation="MTLALVAAAPWIIIPLAAIWRARGDPSLDREPDANPADAPKVSV
                     VIPARNEGGNIAACVQSVLLSRYPELEVIVVDDHSTDDTLAIARVMASFDDRVKVVEA
                     PPLPDGWFGKQWACDRGAAVSSGEMLCFTDADTRHGRDLLGRAVHRLLDRHLDFLSVL
                     GRQVMLTFWERLIQPQVFAMLAMRFGGARRINESKHAIDKIANGQFMLITRAAYEDIG
                     RHASVRDQVAEDLALAQRLFQRGKRTELVEGRHHLSTRMYTSLGSLVRGWMKNIYAGG
                     VRAAPFGSIGRALLPLALLSGPLASIAPPVVLVLAIFGLVGGTWLVWSLIATGAMAIW
                     WIQAYVRAPVAGPMRALRFAAIYPLGSLIVAYIVVRAVVRGRRVEWRGREYVAG"
BASE COUNT          313 a          700 c          671 g          406 t
ORIGIN      
        1 ccgcgccaac acgcggccca cggcggccgc gcgcacgtcg tcgctgccta acgaatcgcc
       61 cgcgcccggc gacacccttg gcctactcct ccagcggtcc gcccgcgttc acccacgcca
      121 tgagtccgcc ttccaacgat atcacgtcgg cgtaccccaa ctcggcaagc gtcgccgccg
      181 ccgtcgccga ccggcctccg cgcgcgcagt acaggatcac cgtgcggtcg gcctcgagcc
      241 ggccgggcgc ctcgtcccgc accatcgcca atggaatgtg caccgccccg ccaatgcgga
      301 acaggttcca ctcgtgctgc tcccgaacat cgacgaacgt gacgtcgtcg ccacgctccc
      361 gcatcgcgat caccgccgac gccggcacct cgcggaacgt ctttcccgaa ggctgcgcgc
      421 tcacgcttgc tccaccatct cgctcccgtg acgaccgatc caatccttat atctatttcg
      481 gcggtccgtt acccgttgtc cagcactcac agctgggcgc gacgcgcgcc gaggaaggta
      541 gcgctcgggc ttccccgggc gcaaaaatgt ccactacctt tccctcgtga cgacagccaa
      601 gcgagacgct ttcgccgagg ccacgtgttg acgcttgcgc tcgtcgctgc ggcgccgtgg
      661 atcatcatcc ctcttgccgc gatctggcgc gcccgcggcg atccctcgct cgaccgggag
      721 ccggacgcaa atccggctga tgcgccaaag gtgtccgtcg tgattcccgc ccgcaacgaa
      781 ggtggaaata ttgccgcctg cgtgcaatcc gtcttactgt cccgctatcc cgagctcgag
      841 gtgatcgtgg tcgacgatca ttcgaccgac gacaccttgg cgatcgcgcg ggtcatggcc
      901 tccttcgatg atcgcgtgaa agtcgtggag gcgccaccat tacccgacgg atggttcggc
      961 aaacagtggg cgtgcgaccg cggtgcggcc gtatcgtcgg gcgaaatgtt gtgctttacg
     1021 gacgcggaca cccgtcatgg gcgcgacctg ctcgggcgcg ccgtacatcg gttgctcgac
     1081 cgacacctgg attttctgag tgtgctgggc cgacaagtga tgctcacgtt ctgggaacgg
     1141 ctgattcagc cgcaggtgtt cgcgatgctg gcaatgcggt tcggtggtgc gcgccgcatc
     1201 aacgaatcga agcacgcgat cgacaagatc gcgaacggcc agttcatgct catcacacgt
     1261 gcggcgtacg aggatatcgg ccggcacgcg tcggttcgcg atcaggttgc ggaggacctg
     1321 gcgttggcgc aacgcttgtt ccagcgtgga aagcgtaccg agctcgtgga gggtcgtcat
     1381 cacctttcga cgcgtatgta cacgtcgctc ggctcgcttg tgcgcggatg gatgaagaac
     1441 atttacgcgg gcggcgtacg ggccgcgcca ttcgggtcga tcggtcgcgc gctgcttccg
     1501 ctggcgctgt tgtcggggcc cttggcgagc atcgcgccgc cggtggttct cgtcctggcg
     1561 atattcggtt tggttggcgg cacgtggctc gtgtggtcac tgattgccac gggggcgatg
     1621 gcgatctggt ggatacaggc gtacgtgcgc gcgccggtag cggggccgat gcgcgcgctc
     1681 cggttcgccg caatttaccc gctgggctcg ttgatcgtgg cgtacatcgt ggtgcgcgcg
     1741 gtcgtacgcg ggcggcgggt cgagtggcgc ggccgggagt atgtcgccgg ttgaggcgga
     1801 aacctttttc ggccggcgct cgtcatatga attgaagcct tcgggcttct acttcctccg
     1861 agtgggggcg cctcccatgc ctgtgccgac aggtatgcgg aggcgttcca tttgcatccc
     1921 gatgtatccc gcgcttatcg ctgccccacc ggcggcgccg ccgcgttctt ccatttctgg
     1981 aaccaggcca gcacgtaggc gatcttggtc atcatgtgac tgggcgtacc cgagatctcg
     2041 tgcgatgcgt taggcacccg gaccagggcc gccggcacct tgcggagctt
//
LOCUS       DASRVO010000503         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_143794, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000503 DASRVO010000000
VERSION     DASRVO010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143794"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFF05_06250"
     CDS             <1..531
                     /locus_tag="VFF05_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZW51441.1"
                     /translation="IRSFNGKTPKIHDSAFIADNAVIIGDVEIGPEANIWYGVVLRGD
                     VNYIRIGARTNVQDGAVLHVTHDGPYTPGGFPLVIGADVTVGHSAILHACTVEDACLI
                     GMHATVLDGAVVRKHAFVAAGAVVAPGKIVGEGELWMGNPARCVRKLDAAQIEALYYS
                     AKHYVKLKDEYLSAAR"
     gene            complement(515..772)
                     /locus_tag="VFF05_06255"
     CDS             complement(515..772)
                     /locus_tag="VFF05_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011050259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZW51442.1"
                     /translation="MDCRAGCGACCIAPSISSPIPGMPNGKPAGVRCIQLDESNRCQL
                     FGKPERPQVCRRLKPKPAMCGANRDEALAILTRLEIASAPR"
     gene            complement(772..1617)
                     /gene="aroE"
                     /locus_tag="VFF05_06260"
     CDS             complement(772..1617)
                     /gene="aroE"
                     /locus_tag="VFF05_06260"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011257342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZW51443.1"
                     /translation="MSDPKFAVFGHPISHSLSPRIHAAFGKQLGIALDYAAIDAPPET
                     FATQLEAFGKAGGSGANITLPLKQVVLPLCAEVSDFARRVGAANTLTRRLDNHWRGDN
                     TDGAGLVRDITQRHGLDLRERRSLILGAGGAARAAAFALLDAGVAELTIVNRTPERAD
                     ALADAIGEPDRVHTRYWNDLASLGNFDLIVNATSAGHARESLPLPFALVAPRALCYDL
                     SYGLAASAFLAWARAAEAGFAHDGLGMLIEQAAESFAIWHGRRPDTDAVYAQLRDELP
                     LHASD"
     gene            complement(1614..>2220)
                     /gene="hemB"
                     /locus_tag="VFF05_06265"
     CDS             complement(1614..>2220)
                     /gene="hemB"
                     /locus_tag="VFF05_06265"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZW51444.1"
                     /translation="HGQDGLIDDAGYVVNDATVAALVKQALSHADAGADVVAPSDMMD
                     GRIGAIRRALEEAGHIHTRILAYSAKYASGYYGPFRDAVGSAVNLGKSNKYTYQMDPA
                     NSDEALREVELDLAEGADMVMVKPALPYLDVVRRVKERFGAPTFAYQVSGEYAMLKAA
                     SLNGWLDERTCVLEALTGIKRAGADAILTYFALQAASWLRE"
BASE COUNT          364 a          710 c          774 g          372 t
ORIGIN      
        1 atccgcagct tcaacggcaa gacgccgaaa atccacgaca gcgccttcat cgccgacaac
       61 gccgtcatca tcggcgacgt cgagatcggg cccgaggcca acatctggta tggggtggtg
      121 ctgcgcggcg acgtcaacta catccgcatc ggcgcgcgca ccaacgtgca ggacggcgcc
      181 gtgctgcacg tgacgcacga cggcccatac acgcccggcg gattcccgct cgtcatcggc
      241 gcggacgtca ccgtcggtca cagcgcgatc ctgcatgcct gcacggtcga ggacgcctgc
      301 ctgatcggca tgcacgccac cgtgctcgac ggcgccgtgg tgcgcaaaca cgccttcgtt
      361 gccgccggtg ccgtcgtcgc gccgggcaag atcgtcggcg agggcgagtt gtggatgggc
      421 aatcccgcgc gctgcgtgcg caagctcgac gctgcgcaga tcgaggcgtt gtactactca
      481 gcgaagcatt acgtgaaatt gaaggacgaa tacctcagcg cggcgcgcta gcgatttcca
      541 gccgcgtcag aattgcaagc gcctcgtcgc gatttgcacc gcacattgcc ggtttgggtt
      601 tcaggcgccg gcagacttgc gggcgctcgg gcttgccgaa cagctggcag cggttggatt
      661 cgtccagttg aatgcaacgc acgccggcgg gtttgccgtt cggcatgccg gggatcggcg
      721 aggaaatcga cggcgcgatg caacaggcgc cgcagccggc tcggcagtcc atcaatcgct
      781 cgcgtgcagc ggcaattcgt cgcgcagttg cgcgtagacc gcgtcggtat ccggacgcct
      841 gccatgccag atcgcgaagg attcggcggc ctgttcgatc agcatgccca ggccgtcgtg
      901 cgcgaagccg gcctcggcgg cgcgcgccca ggcgaggaat gcactcgcgg cgaggccata
      961 ggaaaggtcg tagcaaagcg cacgcggcgc gacgagcgca aaaggcagcg gcagcgactc
     1021 gcgtgcgtgg ccggcggaag tggcattcac gatcaggtcg aaattgccga ggctggcgag
     1081 atcgttccag tagcgcgtgt gcacgcggtc gggctcgccg atcgcatcgg ccagcgcgtc
     1141 ggcgcgttcc ggcgtgcggt tgacgatggt gagttccgcg acgccggcgt cgagcagggc
     1201 gaacgcggcc gcccgcgcgg cgccgcccgc gccgaggatc aggctgcggc gctcgcgcaa
     1261 atccaggccg tggcgctgcg tgatgtcgcg cacgagtccg gcgccatcgg tgttgtcgcc
     1321 gcgccagtgg ttgtcgaggc ggcgcgtgag cgtgttggcc gcgccgacgc ggcgtgcgaa
     1381 gtccgacacc tctgcgcaca acggcagcac gacctgcttc agcggcaggg tgatgtttgc
     1441 gccgctgccg ccggccttgc cgaaggcttc gagttgggtg gcgaaagttt ccggcggtgc
     1501 gtcgatcgcc gcgtagtcga gcgcgatgcc gagttgcttg ccgaatgcgg catggatgcg
     1561 cggggacagc gaatgcgaga taggatggcc gaagacggca aattttggat cgctcattcg
     1621 cgcaaccacg aagcagcttg gagcgcgaag taggtcagga tcgcatctgc gccggcgcgc
     1681 ttgatgcccg tcagcgcttc gagcacgcag gtgcgctcgt ccagccagcc gttgaggctc
     1741 gccgccttga gcatcgcgta ttcgccgctg acctgatagg cgaacgtcgg cgcgccgaag
     1801 cgttccttca cccggcgcac gacgtcgagg tagggcagcg caggcttgac catcaccatg
     1861 tccgcgcctt cggcgagatc gagttcgact tcgcgcagcg cctcgtcgga attcgccggg
     1921 tccatctggt aagtatattt gttgcttttt cccagattca ccgccgagcc gacggcgtcg
     1981 cggaacgggc cgtaataacc tgatgcgtat ttcgccgagt aggcgaggat gcgcgtgtgg
     2041 atgtggccgg cctcttccag cgcacggcgg atcgcgccga tgcggccatc catcatgtcc
     2101 gagggtgcga ccacgtccgc gccggcgtcg gcatgcgaca gtgcctgctt gaccagggcc
     2161 gcgacggttg cgtcgttgac cacgtagccg gcatcgtcga tgagtccgtc ctggccatgc
//
LOCUS       DASRVO010000504         1230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_169310, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000504 DASRVO010000000
VERSION     DASRVO010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169310"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VFF05_06270"
     CDS             complement(<1..380)
                     /locus_tag="VFF05_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZW51445.1"
                     /translation="MAQPVPLPRGVHHLALCTDDMKATVDFYVDVLGMPLVHAMRVPP
                     GLGTGPRNRGNPPYEELRHYFFDMGNDSLLAFFEIPNGEEPRGNRNAIGAMQHCAFVV
                     TPARLREIEQRLNARGIDYIGPIA"
     gene            complement(549..809)
                     /locus_tag="VFF05_06275"
     CDS             complement(549..809)
                     /locus_tag="VFF05_06275"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HZW51446.1"
                     /translation="MSLINLLVSARQALVQHRQRCRAFDELMSLDDRALADIGIHRSQ
                     IPALVAGVEPAAGYDAEPQAAIGPAFNCGPRLATGRPFLPPL"
BASE COUNT          220 a          383 c          427 g          200 t
ORIGIN      
        1 tgcgcgatcg ggccgatata gtcgatgccg cgcgcgttca gccgctgttc gatctcgcgc
       61 agccgcgccg gcgttacgac aaaggcgcag tgctgcatcg cgccgatcgc gttgcggttg
      121 ccgcgcggtt cctcgccgtt cgggatttcg aagaacgcca ggaggctgtc gttgcccatg
      181 tcgaagaaat agtggcgcag ctcttcatag ggcgggttgc cgcgattgcg cggcccggta
      241 ccgagccccg gcggtacccg catcgcatgc accagcggca tgccgagtac atcgacgtag
      301 aaatcgacgg tcgccttcat gtcgtcggtg cacagcgcca ggtggtggac gccgcgcggc
      361 agtgggacgg gttgcgccat gatttcctcc tagtgctccg actcagtcag ttggtacatg
      421 cggcccaccg ttcgtcatgc ccggacttga tccgggcatc cacggaaacc ggcccgcctg
      481 cgccgctcga cgtggatggc cgggacaaac ccggccatga cggtgaatct tgtgtctgtg
      541 tctgagcact agagcggcgg cagaaacggc cggccggtgg caaggcgcgg gccgcagttg
      601 aaggccgggc cgatcgccgc ctggggctct gcgtcatacc ccgcggccgg ttccacgccg
      661 gcgaccagcg ccgggatctg cgaacggtgg ataccgatgt cggcgagcgc gcggtcgtcg
      721 agcgacatca gctcatcaaa ggcgcggcac cgctgccgat gctgcaccag cgcctgacgc
      781 gccgaaacga gcaaattgat cagcgacatt gtcgaactcc cgcaccgaaa tggtgcaacg
      841 cacaaatggc gccatcagat cgcgtgcgcc agatatccgg gcgcaacaca gccatgcaag
      901 cgcggcccta tcggcttatt tagcctgcat tgttgcatag cagcatgatg aagagcgcaa
      961 ggctttcggc gcgggcgcgg cgtgacttcg ggattgcgtc ggtgggcgcg caatgacagg
     1021 ctctaaagag gggaacggac gcgacgggac gaacgaggcg atgtcgaaca ggagcgagcg
     1081 atgacaacaa cggcgatgcc ggatcgcgac cacctggcgc agctgctgca gggcgcagga
     1141 tcgcgcttcg atccggacgg cgcggccgcg ttgatcgccg gcgtgctggc ggcgccgccg
     1201 gagatcggca cgagttggca tgtgctggtc
//
LOCUS       DASRVO010000505         1347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_169329, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000505 DASRVO010000000
VERSION     DASRVO010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169329"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /gene="raiA"
                     /locus_tag="VFF05_06280"
     CDS             complement(<1..300)
                     /gene="raiA"
                     /locus_tag="VFF05_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZW51447.1"
                     /translation="MQLTVTGKQVDVGDALRRHVEDALDTLLGKYFRTAIEAHAVFSR
                     EAHLIRADVSLHVGRGIVVNSSAAAPDPYVSFDGAGERLGKQLRRYKRRLRDDHGK"
     gene            complement(402..>1347)
                     /gene="rpoN"
                     /locus_tag="VFF05_06285"
     CDS             complement(402..>1347)
                     /gene="rpoN"
                     /locus_tag="VFF05_06285"
                     /inference="COORDINATES: protein motif:HMM:NF004596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZW51448.1"
                     /translation="RLGCPVERIERVLARLQELDPPGIFARDLAECLALQLRDRNRLD
                     PAMQLLLDNLPLLAARDAGALMRICGVDAEDFADMVADIKSLDPRPGHAFDLPLAQPV
                     VPDIIMRALPQDGWTVELNAETLPRVLVNNRYFARVNSAARNKIERDYLADRLQAANW
                     LVKSLHQRATTILKVAAEIVHQQDGFFRNGVQALRPLILRDIADAIGMHESTVSRVTT
                     NKYMATPRGLFELKYFFTSSIPASQGGESHSAEAVRFRIRSMIDGEPSDDTFSDEKIV
                     ELLQREGVDIARRTVAKYREAMRIPSSVQRRREKTLAL"
BASE COUNT          242 a          431 c          438 g          236 t
ORIGIN      
        1 cttgccgtga tcatcgcgca gccgacgctt gtagcgccgc aattgtttgc ccaagcgctc
       61 tccggcgcca tcgaaggaga catagggatc tggcgccgcc gcgctgctgt tgaccacaat
      121 gccgcgcccg acatgcagcg atacatcggc gcggatcaaa tgcgcttcac gggaaaacac
      181 ggcatgggct tcgatcgcgg tgcggaaata tttacccagc agggtgtcaa gggcgtcctc
      241 gacgtggcgg cgtaaggcgt cgccgacatc gacctgcttg cccgtgaccg tcagctgcat
      301 ggccttacac catcaaaatt cagggcagcg gcgcacaacg gcatgcgcag acgaaattgg
      361 ggatcgccgc cgctgcggtc aagccgcgcc ggcggcgctg gtcacagggc taacgtcttt
      421 tcgcggcggc gctgcaccga tgaggggatg cgcatcgcct cgcgatactt tgccacagtg
      481 cggcgcgcaa tatcgacgcc ctcgcgctgc agcagctcga cgattttctc gtcggaaaat
      541 gtgtcgtccg acggctcgcc gtcgatcatc gagcggatgc ggaagcgcac cgcctcggcc
      601 gaatgcgatt cgccgccttg cgatgccggg atcgacgagg tgaagaaata cttcagttcg
      661 aacagcccgc gcggcgtcgc catgtatttg ttggtggtga cgcggctgac cgtgctctcg
      721 tgcatgccga tcgcatcggc gatgtcgcgc aggatcaacg ggcgcagcgc ctggaccccg
      781 ttacggaaga aaccgtcctg ctggtgcacg atctcggccg cgaccttgag gattgtcgtg
      841 gcgcgctggt gcagcgattt gaccagccag ttcgccgcct gcaaccggtc cgccaggtag
      901 tcgcgctcga tcttgttgcg cgcggcgctg ttgacgcgtg cgaaatagcg gttgttaaca
      961 agtacgcgag gcagggtttc ggcattcagt tcaaccgtcc aaccgtcttg cggcagagcc
     1021 cgcatgatga tgtcgggcac caccggctgg gccagcggca gatcaaaggc gtgtcccggc
     1081 cgcgggtcga gcgacttgat gtcggcgacc atgtcggcga aatcctcggc gtcgacccca
     1141 cagatccgca tcagcgcgcc ggcgtcgcgc gccgccagca gcggcagatt atcgagcaac
     1201 agctgcatcg ccgggtcgag ccggttgcga tcgcgcaact gcagcgccag gcattccgcc
     1261 agatcacggg cgaagatgcc cggcggatca agctcctgca atcgtgccag gacccgttcg
     1321 atgcgctcga ccgggcagcc cagccgg
//
LOCUS       DASRVO010000506         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_169345, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000506 DASRVO010000000
VERSION     DASRVO010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169345"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="VFF05_06290"
     CDS             complement(<1..1273)
                     /locus_tag="VFF05_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW51449.1"
                     /translation="MNRNEIIADLSESVRNLLQQCDAALRAGDRARALQCARRALQDA
                     PGHPEALRQLAAALSAHGPSAEAMAAIEQALAFYPDDPAVLTTHALVMQANGRLDQAV
                     ESFRLASELVPDSANNAYNFGRALFRHGEESESLGALERALAIDPEHRNARATAAEVL
                     VRLGRRDEAAVHLRHLIERDSTDVRSWAALATHRAAAIDAGDLAAMSALFENPSTGAE
                     DRVRLGFALGKLRAERAEYAESFSAYVRANALVRARVAWNAVGHARAVDELLDAFAVP
                     AGVDGARGSEMLFVVSLPRSGSTLVEQIIAAHPQVDAGDERNDVFNVIEDESRRRKQP
                     LGQWAKLATAADWQRLGEDYLARSRRWRADGKHATDKLPSNWLWLGAIAAMLPGARII
                     DCRRARIETAWSCFCHLFRIGTQDFSYDFASI"
     gene            complement(1260..2198)
                     /locus_tag="VFF05_06295"
     CDS             complement(1260..2198)
                     /locus_tag="VFF05_06295"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW51450.1"
                     /translation="MAGFALAIALEHEGDVTAAFEQMRASHALSREREGPYEIDPISR
                     HVDDVLDAFDPVPAGAGNAQGKEAIFVVSMPRSGSTLTEQILASHSQVDGGGELPDFL
                     QVVMDESDRAKESFPAWVSNQTPEKWRMMGARYLARTRRFRVRRPRFTDKRLDNWLMT
                     GAILASLPEARIVVVRRDPLENCLACYRYMVTHGYTHTFADLATTWRNFDRAVTRWQE
                     LYPGRVYVQVYEDLVAEPEKQIRALLDYCDLPFEEACLNFHATERIVATPSATQVREP
                     IRRNTARADKYGALLDPLRKELGMPPWRSEDAHEPQ"
BASE COUNT          418 a          763 c          731 g          414 t
ORIGIN      
        1 caatcgaggc gaagtcgtag ctgaaatcct gcgttccgat gcggaacaag tggcagaagc
       61 acgaccacgc ggtctcgatc cgagcgcgtc ggcagtcgat gatgcgcgcg cctggcaaca
      121 tggccgcgat cgcgccgagc cacagccagt tgctcggcaa tttgtcggtc gcatgcttgc
      181 cgtcggcgcg ccaacgccga ctgcgtgcga gatagtcttc cccaagccgt tgccaatcgg
      241 cggcagtggc gagtttcgcc cattgcccca acggttgctt gcgacgacgg ctctcgtctt
      301 cgatgacgtt gaacacgtcg ttgcgctcgt caccggcgtc gacctgcgga tgcgcggcga
      361 tgatctgctc gacgagcgtc gagccggaac gtggcaggct gacgacgaaa agcatttccg
      421 atccgcgcgc gccgtcgacg ccagccggca cggcaaaggc atcgagcaac tcgtcgaccg
      481 cacgcgcatg ccccaccgca ttccacgcca cacgtgcgcg caccaacgcg ttggcgcgta
      541 cgtaggcgga gaaggattcg gcatattccg cacgctcagc acgcagcttg ccgagggcga
      601 atcccaggcg tacccgatct tcggcgccgg tcgacggatt ctcaaagagc gcagacatcg
      661 ccgcgagatc gccggcatcg attgcggctg cgcggtgtgt cgccaacgcg gcccaggagc
      721 gcacgtcggt cgaatcgcgt tctatcagat ggcgcaagtg cacggcagcc tcgtccctgc
      781 ggcccaggcg aaccaacact tcggcggcgg tggcacgtgc attgcggtgc tctgggtcga
      841 tcgccagcgc gcgttccagc gcgcccaggc tctcgctttc ctcgccatgg cggaacagcg
      901 cccggccaaa gttgtaggca ttgttcgccg agtccggaac cagctcgctc gccagacgga
      961 acgattcgac ggcctggtcg aggcggccgt tcgcctgcat cacgagcgcg tgcgtggtca
     1021 gcactgccgg atcgtccgga tagaatgcca gggcttgctc gatggcggcc atggcctcgg
     1081 ccgaaggtcc atgcgcgctc agtgccgcag ccagttgccg cagcgcttca ggatgtccgg
     1141 gcgcatcctg caacgcccgc cgtgcgcatt gcagtgcccg cgcccggtcg cccgcgcgca
     1201 aggcggcatc gcattgctgc aataggttgc gcaccgactc ggacagatcg gcgatgattt
     1261 cattgcggtt catgggcatc ctccgaccgc cacggcggca ttcccagctc cttgcgcagc
     1321 ggatcgagca aggcgccgta tttgtccgcc cgtgccgtgt tgcggcgaat cggttcgcgc
     1381 acctgggtag cgctgggcgt tgcgacaatg cgctccgtcg cgtggaaatt caggcacgct
     1441 tcctcgaatg gcaaatcgca atagtcgagt agcgcgcgaa tttgtttctc aggttctgca
     1501 accagatctt cgtaaacttg gacatacacg cggccagggt acaactcctg ccaacgcgtg
     1561 acggcacgat cgaaattgcg ccacgtggtg gcgagatcgg caaacgtatg cgtgtagccg
     1621 tgcgtcacca tatagcggta gcaggccagg caattttcca acggatcgcg gcgcaccacg
     1681 acaatgcgcg cttccggaag gctagccaag atcgcaccgg tcatcagcca gttgtccagg
     1741 cgcttgtcgg tgaaccgtgg ccggcgcacg cgaaaacgcc gcgtccgcgc cagataacgc
     1801 gcgcccatca tccgccactt ttcgggtgtc tgattggaga cccaggccgg aaacgattct
     1861 tttgcgcgat cggactcgtc catgaccacc tgcagaaaat ccggcaattc gccgccaccg
     1921 tccacctgcg aatgcgaggc gaggatctgc tctgtcaggg tcgagcccga acgtggcatg
     1981 ctgacgacga aaatcgcttc ctttccctgc gcattgcctg cgccggccgg aaccggatcg
     2041 aatgcatcga ggacatcgtc gacatggcgc gatatcgggt cgatttcgta gggtccttcg
     2101 cgttcgcgcg agagtgcgtg ggatgcgcgc atctgttcga acgcggcggt gacatcgccc
     2161 tcgtgttcga gcgcgatcgc cagagcgaat ccggccaagg cacgtttgtt cgacggcacc
     2221 gacatgtcgt tcacgacgcg ccgcatcgcg gcgacgtccg catccgtcaa cggcatcggt
     2281 ttcatggtcg cgtaccacca ccacgtagac ccggccccgg cgggat
//
LOCUS       DASRVO010000507         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_196755, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000507 DASRVO010000000
VERSION     DASRVO010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196755"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="VFF05_06300"
     CDS             <1..970
                     /locus_tag="VFF05_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZW51451.1"
                     /translation="SMAKGLLPDTHAQSASACRSYVLAEADVVMLIGARLNWLLSHGK
                     GKTWGGPKQFIQIDIAPTEIDSNVAIAAPVIGDIGSCVSALLAGIGSGFAKPDAAWTG
                     AIAERKEKNMAKMAATLAQNPSPMNYHSALRAIRDVLKTRPDINVVNEGANALDFTRS
                     IVDMYQPRKRLDAGTWGIMGIGMGYSVGAAVTSGLPVVAIEGDSAFGFSGMELETICR
                     YQLPVTTVIFNNNGVYKGCDPNLNPNKADVAVTAFVKGARYDKMIEAFGGVGYHVTTP
                     AEMTKALTEAIAAGKPALINAVIDEAAGTESGRLTNLNPKTAAAKK"
BASE COUNT          209 a          337 c          332 g          162 t
ORIGIN      
        1 gtccatggcg aaaggcctgc tgcccgacac gcatgcgcaa tcggcttcgg cctgccgctc
       61 ctacgttctg gccgaggccg acgtggtgat gctgatcggc gcccgcctga actggctgct
      121 ttcgcatggc aagggcaaga cctggggcgg accgaagcag ttcatccaga tcgacatcgc
      181 accgaccgaa atcgacagca acgtggcgat tgccgccccc gtgatcggcg acatcggctc
      241 ctgcgtgtcg gcgctgctgg cgggcatcgg ttccggcttt gccaaaccgg acgccgcatg
      301 gaccggcgcg atcgcggagc gcaaggaaaa gaacatggcg aagatggcgg ccacgctcgc
      361 gcagaatccg tcgccgatga actaccacag cgcgctgcgc gcgatccgcg atgtgctgaa
      421 gacgcggccg gacatcaacg tcgtcaatga aggcgccaat gccctcgatt tcacccgcag
      481 catcgtcgac atgtaccagc cgcgcaagcg tctggacgcc ggcacctggg gcatcatggg
      541 cataggcatg ggttattccg tcggcgcggc ggtcacgagc ggcctgcctg tcgttgcgat
      601 cgaaggcgac agcgcgttcg gtttcagcgg catggaactc gagactatct gccgttacca
      661 gctgccggtc accaccgtga tcttcaacaa caacggcgtc tacaaaggct gcgatcccaa
      721 cctcaacccc aacaaagccg atgtcgcggt gaccgccttc gtcaagggcg cgcgctacga
      781 caagatgatc gaggctttcg gcggcgtcgg ctatcacgtc accacgccgg cggaaatgac
      841 caaggctttg accgaagcga tcgcggcggg caagccggcg ctcatcaacg ccgtcatcga
      901 cgaagcggcg ggtaccgaga gcggtcgact gaccaatctg aatccgaaaa ctgcggcggc
      961 caagaagtaa gccgcggggc catggcgagg ccatccgccg aaggttcggc ggccggctcc
     1021 gttgccgacg catggtgcaa
//
LOCUS       DASRVO010000508         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_196812, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000508 DASRVO010000000
VERSION     DASRVO010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196812"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1049
                     /locus_tag="VFF05_06305"
     CDS             <1..>1049
                     /locus_tag="VFF05_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51452.1"
                     /translation="GYSERIYQVFGRETLDQLVPLAAEGPLERTGLPQPPPWVRARLE
                     AERIKHGEDELTIAEAAAENPGVLRAHGFISKPEIQKLNRNSIFIFVNGRLIRDRLIL
                     HALTEAYRNIMPPTVYPVVLLFLEIPFTEVDVNVHPSKTEVRFRQQSFVHDFVRESVR
                     AALMKARPIPQFAAEIHAAPTAAAGLTPGARMSQQVEDIAAKAGIIGDPGGTEADAQF
                     ALHPPAPPAINQSLGFGADPIRVDANAAIPVARLEPQTFGGTVVRERSGTSCGIGAAD
                     AQVEDAAANFADTPVLATLKPLGQVRDSFILAVNDEGLWIIDQHVAHERVLFEKVLRQ
                     RAVEHVESQRMLMPL"
BASE COUNT          183 a          371 c          307 g          188 t
ORIGIN      
        1 gcggctactc ggaacgcatt taccaggtct ttggccgcga aactctggac caactggtcc
       61 cgctcgccgc tgaagggccg ctggagcgca ccggtctgcc gcagcctccg ccgtgggtgc
      121 gtgcgcgatt ggaagccgag cgcatcaagc acggcgaaga cgaactcacg attgccgaag
      181 ccgccgccga gaaccccggc gttctgcgcg cgcacggctt catctccaaa ccggagatcc
      241 agaagcttaa ccgcaattcg atcttcatct tcgtcaacgg ccgtctcatc cgcgaccgcc
      301 tcatcctgca cgcgctgacc gaagcctatc gcaacatcat gccgcccacg gtctatcccg
      361 tggtgctgct ttttctcgaa ataccgttca ccgaagttga cgtcaacgtc catccgtcga
      421 agaccgaggt acgcttccgt cagcagagtt tcgttcacga tttcgtacgc gaatcggtcc
      481 gcgcggccct gatgaaggca cgccccatcc cgcaattcgc ggccgagatc cacgccgcgc
      541 ccacggccgc cgccggactc acgcccggcg cgcgtatgtc gcagcaggtt gaggacattg
      601 ccgccaaggc gggcatcatt ggcgatccgg gcggcacgga agccgatgcg cagttcgctc
      661 tgcatccgcc cgcgccgccg gctatcaacc agtcgctcgg gtttggcgca gaccccatca
      721 gggtcgacgc aaacgccgcc atccccgtcg cgcgccttga accgcagact ttcggcggta
      781 ccgtcgtgcg ggagcgttcg ggcacgtcct gcggcatcgg tgcggccgac gcgcaggtcg
      841 aggacgctgc cgccaacttc gctgatacgc ccgttctcgc cacgctcaag ccgctcggcc
      901 aggttcgtga ctcctttatt ctcgccgtca acgacgaagg gctctggatc atcgatcagc
      961 acgttgcgca cgagcgtgtg ctttttgaga aagtcctgcg tcagcgcgcg gtcgagcacg
     1021 tcgaaagcca gcgcatgctg atgccgctc
//
LOCUS       DASRVO010000509         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_92078, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000509 DASRVO010000000
VERSION     DASRVO010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92078"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1072
                     /locus_tag="VFF05_06310"
     CDS             47..1072
                     /locus_tag="VFF05_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZW51453.1"
                     /translation="MNARLQSLAENPLSNPLLDAAVLKRSATNVVDIEARAIEALRTR
                     IDDNFLRACQLMFDCPGRVIVSGMGKSGHIARKMAATLASTGTPAFFVHPGEASHGDL
                     GMITQKDVVLALSNSGETDELLTILPVIKRQGIPLVAMTGNPASSLAAMGDVHLDVSV
                     PAEACPLNLAPTASTTAALVMGDALAIALLEARGFTDEDFARSHPAGSLGRRLLLHIA
                     DVMHMGEAVPSIGATASLSEALMEMTRKGLGMTAIVDDAGTLLGVFTDGDLRRAVDNQ
                     TIDLRTTPVTALMTAHPKTIGPDKLAVEAARLMEAHKIHALLVVDADHRVVGALNIHD
                     LLRARVV"
     gene            1075..1623
                     /gene="kdsC"
                     /locus_tag="VFF05_06315"
     CDS             1075..1623
                     /gene="kdsC"
                     /locus_tag="VFF05_06315"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HZW51454.1"
                     /translation="MPYPHLSDLPADIRERAAKIRLALFDVDGVLTDGRLYYSDDGRE
                     SKAFHVHDGLGLKRLMANGIEVGIITARLSHMVTERTAELGIAHVYQGQENKLACYEQ
                     LIHALKLTPEQCCYCGDDLPDLAIMQRVGLSIAVANAHAWVRERARWRTRSAGGEGAV
                     REVCDLLLAAHGKAESELARFA"
     gene            1623..>1864
                     /locus_tag="VFF05_06320"
     CDS             1623..>1864
                     /locus_tag="VFF05_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HZW51455.1"
                     /translation="MERRLVFAIAALAVLALSTQILVWVFAPRETQSAFVGPPRSDYT
                     LSGFSIDALDAQGQHSFSVAGPRLVRRAEDASIFVTT"
BASE COUNT          316 a          676 c          575 g          297 t
ORIGIN      
        1 cgccatttcc ctgctcaatc ccgccttcct gcccggtcca tacgtcatga acgcccgcct
       61 gcagtcgctt gccgagaacc cgctgagcaa cccgttgctc gacgcggcgg tactcaagcg
      121 cagcgcgacc aatgtcgtcg acatcgaggc gcgcgcgatc gaagccttgc gcacgcgcat
      181 cgacgacaac ttcctgcgcg cctgccagct catgttcgac tgccccggcc gcgtgatcgt
      241 ttccggcatg ggcaaatccg gccacatcgc gcgcaagatg gccgcgacgc tggcatccac
      301 cggcacgccg gcgttttttg tgcatccggg cgaagccagc cacggcgacc tgggcatgat
      361 cacgcaaaaa gacgtggtgc tggcgctgtc caattccggc gaaaccgacg aactgctcac
      421 catcctgccg gtaatcaagc gccagggcat tccgctggtg gcgatgaccg gcaatccggc
      481 ttcctcgctc gccgcgatgg gcgacgtgca cctggatgtg agcgttccgg ccgaggcctg
      541 cccgctcaat ctggcgccca ccgccagcac gacggcggcg ctggtcatgg gcgacgcgct
      601 cgccattgcc ctgctcgaag cgcgcggctt caccgacgag gatttcgcac gttcgcatcc
      661 ggccggaagc ctgggccggc gcctgctcct gcacatcgcc gacgtgatgc acatgggcga
      721 agcggtaccc agcatcggcg ccaccgcaag cctgtccgaa gcgctgatgg aaatgacccg
      781 caagggtttg gggatgaccg ccatcgtcga cgatgcgggc accttgcttg gcgtgttcac
      841 cgacggcgac ttgcgccgtg ccgtggacaa ccagaccatc gacctgcgca ccacgccggt
      901 caccgcgctg atgaccgcac accccaagac catcgggccc gacaaactcg ccgtggaggc
      961 cgcgcgcctg atggaagcgc acaagatcca cgctttgctc gtggtcgatg cggaccaccg
     1021 cgtcgttggt gcgctcaaca tccacgacct gctgcgcgcg cgcgtggtct gaagatgccg
     1081 tatccgcatc tcagcgacct gcccgccgac atccgcgaac gcgctgcgaa aatccgcctc
     1141 gccctgttcg acgtcgacgg cgtgctcacc gacggccgcc tgtactacag cgacgatggc
     1201 cgcgaaagca aggcgttcca cgtccacgac ggactcggac tcaagcgcct gatggcgaac
     1261 ggcattgagg tcggcatcat caccgcgcgc ctgagccaca tggtcaccga gcgcacggcg
     1321 gaactgggca tcgcgcacgt ctaccagggc caggaaaaca agctcgcctg ctacgaacag
     1381 ctgatccacg cgctcaagct cacgccggaa cagtgctgct actgcggcga tgacctgccg
     1441 gacctcgcca tcatgcagcg cgtcggactt tccattgccg tggccaacgc gcacgcgtgg
     1501 gtgcgcgaac gcgcgcgctg gcgcacgcgc agcgcgggcg gcgaaggcgc ggtacgcgag
     1561 gtctgcgacc tgctgcttgc cgcgcacggc aaggccgagt ccgaattggc caggttcgcc
     1621 tgatggaacg ccgcctcgtc ttcgccatcg cggcgctggc cgtgctcgcg ctgtccacgc
     1681 agatcctggt gtgggtgttc gcgccgcgcg aaacccagtc cgccttcgtc ggaccgccac
     1741 gttcggacta caccctttcc ggtttctcca tcgacgcgct cgacgcccag ggccagcaca
     1801 gcttcagcgt cgccggaccg cgcctggtgc gtcgcgccga ggacgcctcg atcttcgtga
     1861 cgac
//
LOCUS       DASRVO010000510         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_66839, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000510 DASRVO010000000
VERSION     DASRVO010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66839"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1691
                     /locus_tag="VFF05_06325"
     CDS             <1..1691
                     /locus_tag="VFF05_06325"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51456.1"
                     /translation="AENSQQVTQFITGLLQGADPAQAHGRTLSAQDLLEQGARRLYRQ
                     SKIDPDVRARVLQTIAATYTNLGLYDRALPLAQEVLALRRSDPARIDAGIAESMDGLG
                     RIYRLKADYAQAEPLLRGALAMRRAIFPADDPAIIESVDHMAALERAKGDFKDADVLL
                     AEAVQSARRRFGDEAVETARYLDDYAANLDDMGKRAGALALYRQALSIRDKNLGADDP
                     EVANSLLNLGVHLDQSGNFDAAMPLVERALAIRKKIYGPHHPLVAFAEIDVAGVYEDE
                     NRLDAAEGLALDALAVGRASLPPDHPRIGEALNMLAMLRMLRRDYAGAVPMAREVLDR
                     FTKTLGPDHPDTLTAKNNLAYALLHAGRAAEAETLLRDALARKRADNGQGDATDYENL
                     ANALALQGKHAEAVGYAQRAVKIQKQREGEISGNTAVALRVLAMAEASNGAAADAERD
                     FRAVLAIGERLHATQNIDMFQWRLPLADFLVGANRCAEAIPLLIEIIDELRPRLPLRD
                     PLPILQAHLLQGQCMRGKDGAALRMQARRELLKLPSVGVDLYPTSARLLAAKGR"
BASE COUNT          335 a          571 c          596 g          306 t
ORIGIN      
        1 aggcggagaa ttcgcaacaa gtcacgcaat tcatcaccgg attgctgcag ggcgcggatc
       61 ctgcgcaagc gcacggaagg acgctcagcg cgcaggattt gctggagcag ggcgcacggc
      121 gcctgtaccg ccaatcgaaa attgatccgg acgtccgcgc acgggtgctg caaacaatcg
      181 ccgcgaccta cacgaatctg ggtttgtacg atcgcgcctt gccgctggcg caagaggtgt
      241 tggcgttgcg ccgcagcgat cccgcgcgca tcgatgcggg catcgcggag agcatggacg
      301 gcctcggtcg tatttaccgc ctcaaggccg actatgcgca ggcggagccg ctgttgcgcg
      361 gcgcgctcgc gatgcgccga gccattttcc ctgcagatga tccggcgatc atcgaaagcg
      421 tcgatcacat ggccgccctc gaacgcgcca agggcgattt caaggacgcc gacgtgttgc
      481 tggccgaggc cgtgcagtcc gcgcggcgcc gtttcggaga cgaggccgtc gaaacggcgc
      541 gctatcttga cgattacgcg gcgaatctgg acgacatggg caagcgcgcc ggcgccctgg
      601 cgctttaccg gcaggcgctt tcgatccgcg ataagaacct cggcgcggac gatccggagg
      661 tggccaattc cttgctcaac ctcggcgttc acctcgacca atccggcaat ttcgacgctg
      721 ccatgccttt ggtcgagcgc gcactggcga tccgcaaaaa aatctacggt ccgcatcatc
      781 cgcttgtcgc gttcgcggaa atcgacgtcg ctggagttta cgaagacgaa aatcggctcg
      841 acgccgcgga aggactcgca ctggacgctt tggcggtcgg ccgcgccagc ctgccgcccg
      901 atcatcccag aatcggcgaa gcactgaaca tgctggccat gctgcgcatg ttgcgccgcg
      961 attatgccgg cgcggtgccg atggcgcggg aagtgctgga tcgcttcacg aaaacgcttg
     1021 gcccggatca tcccgacaca ctgacggcca agaacaatct cgcctatgcg ctgttgcatg
     1081 ccgggcgtgc ggctgaagcc gaaaccctgc tgcgcgacgc gctcgcgcgc aagcgcgctg
     1141 acaacgggca gggcgacgca accgactacg agaatctggc gaatgcactg gccttgcagg
     1201 gcaaacacgc cgaagcggtc ggctacgcgc agcgcgcggt caagatccag aaacagcgcg
     1261 agggcgagat ttccggaaac accgccgtgg cgctgcgcgt cctggcgatg gccgaagcgt
     1321 cgaatggcgc ggcggcggac gccgaacgcg atttccgcgc ggtgctggcc atcggcgaac
     1381 gtctgcacgc cacgcagaat atcgatatgt tccagtggcg gctgccgctg gcggatttcc
     1441 tggtcggcgc caatcgctgc gcggaggcca taccgctctt gatcgaaatc atcgacgagt
     1501 tgcgtccgcg tctgccgttg cgcgatccgc tgccgatcct gcaggcgcat ctgctgcaag
     1561 gccagtgcat gcgtggcaag gacggcgccg ccctgcggat gcaggcgcgg cgcgaattgc
     1621 tcaagttgcc gagtgtagga gtggatttgt atccaacgtc cgcacgattg ctggcggcga
     1681 aaggacgcta atcgaagccg cttgcgaaga tcacgtcacg cacctgctgc tggagggcgc
     1741 cgatgtcggt ggcgccgttt gccgttcgtg cgaacccgct gccgcgctgg tcgttggcat
     1801 aaccgcag
//
LOCUS       DASRVO010000511         1797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_196849, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000511 DASRVO010000000
VERSION     DASRVO010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196849"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFF05_06330"
     CDS             <1..291
                     /locus_tag="VFF05_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51457.1"
                     /translation="QMPQACCNMLRALNDFLHPRTHVVVRFDGAAEESTWRAAVADRA
                     NRRCDVYFIPRVTGKPPGTLAAQTHAAAGAAYVCRGTQCEAPLHDPSGFKFD"
     gene            complement(298..1368)
                     /locus_tag="VFF05_06335"
     CDS             complement(298..1368)
                     /locus_tag="VFF05_06335"
                     /inference="COORDINATES: protein motif:HMM:NF023701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZW51458.1"
                     /translation="MSPAARRYLLLSPSAQAAYAQLFDAVQALELTRSVANLRGSFAA
                     KTVKGHKYWYFQFSAGGKLRQVYVGPDSPQVRELIERRGDMRPVQALEPLAKSAIVLG
                     CDGMLVSHFRVVARLADYGFFKAGGVLVGTHAFLAFGNMLGVRWGDASRTQDVDFAHA
                     GKNLAIALPSSLKIDTQAAIHSLQMGFLPLTGIDGRAGASWLSARDPDFQLDFLTTLH
                     RGGDAALRHAQLGILLQPLKFMEYLLEDTQQAALFCNDGAVLVNVPHPARYALHKLIV
                     AGERSAARAAKSNKDMRQSAALLEVYGESSAHDVREAWRDLIRRGAGWRERALRGRAA
                     LAKIAPELNVDSLLPAPRMAKR"
     gene            complement(1459..>1797)
                     /locus_tag="VFF05_06340"
     CDS             complement(1459..>1797)
                     /locus_tag="VFF05_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HZW51459.1"
                     /translation="PNGFVGTVHAMDARVGGSYRMTFRHLASGEAHTFGGTYLELVPG
                     ERIRYNDRFDDPNLPGEMHATITLKRVSCGCDIEIVQEGVPDAIPAEMCYLGWQDSLR
                     LLALLVEGSG"
BASE COUNT          354 a          586 c          536 g          321 t
ORIGIN      
        1 caaatgccgc aggcttgttg caacatgttg cgcgcgttga acgatttcct gcatccgcgt
       61 acccacgtcg tcgtgcgctt cgatggcgca gcggaagaat cgacatggcg cgccgccgtc
      121 gccgaccgcg cgaatcgccg ctgcgacgtc tacttcatcc cgcgcgtgac aggaaaaccg
      181 cccggtacgc tggctgccca aactcacgcg gctgccggcg ctgcctacgt gtgccgcggc
      241 acgcagtgcg aggcgccatt acacgatccg tcaggtttca agttcgattg agtggtttca
      301 gcgttttgcc attctcggcg ccggtaacaa cgaatccaca ttcagttctg gagcgatctt
      361 cgccaacgcc gcgcgcccgc gtagcgcacg ttcgcgccaa cccgcgccac gtcggatgag
      421 atcgcgccaa gcctcgcgca catcgtgagc cgaactttcg ccgtagactt ccagcagagc
      481 agcgctttgg cgcatgtcct tgttcgactt tgccgcgcgc gcggcactgc gttcgccagc
      541 gacgatgagc ttgtgcaaag cgtaacgcgc cgggtgcggc acgttgacca gcaccgcgcc
      601 atcgttgcag aacagcgccg cctgctgcgt gtcttccaga agatattcca tgaacttgag
      661 cggctgcaac aggatgccga gctgtgcgtg ccgcaaggca gcatcgccgc cgcgatgcaa
      721 ggtggtcagg aaatcgagct ggaaatccgg atcgcgtgcg ctcaaccacg aagcgcccgc
      781 cctgccatcg atcccggtca atggcaagaa ccccatctgc agcgaatgga tggccgcttg
      841 cgtatcgatt ttgagactcg acggcaatgc aatcgcgaga ttcttgccgg catgcgcaaa
      901 gtcgacgtct tgcgtgcgcg aggcatcgcc ccagcgcacg ccgagcatgt tgccaaaggc
      961 caggaaggca tgtgtaccca ccaacactcc gccggccttg aagaaaccat aatccgccag
     1021 ccgcgccacc acgcggaaat gcgataccag catgccgtcg cagccgagca cgatggccga
     1081 cttggctagt ggctccagcg cctgcacggg acgcatgtcg ccacgtcgtt cgatcaattc
     1141 gcgcacctgt ggcgaatccg gtcccacata gacctggcgc agcttgcccc ccgcgctgaa
     1201 ctggaaatac cagtacttgt gccctttgac cgtcttggcc gcgaacgaac cgcgcaggtt
     1261 ggcaaccgat cgcgtcagtt cgagcgcctg cacggcgtcg aacaattgcg cgtaggccgc
     1321 ctgcgcggac ggcgacagca gaaggtaacg gcgggctgca gggctcatgg cagttatact
     1381 agctacttca caatcttagt ataaccacgc agttatacta aatcaataca ccagttagta
     1441 taaccatcgg cgatgcgctc aaccactgcc ctccaccagc agcgccagca gccgcaggga
     1501 atcctgccaa cccagatagc acatttcggc ggggatcgcg tcgggcacgc cttcctgcac
     1561 gatctcgatg tcgcagccgc aggagacgcg cttgagcgtg atcgtcgcgt gcatttcgcc
     1621 gggcaggttg ggatcgtcga agcggtcgtt gtagcggatg cgctcgccgg gaacgagttc
     1681 gagatacgtg ccgccgaacg tgtgcgcctc accgctggcg agatggcgga acgtcatgcg
     1741 gtagctgccg ccgacgcgcg cgtccatggc atgcacggtg ccgacgaagc cgttagg
//
LOCUS       DASRVO010000512         1571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_377774, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000512 DASRVO010000000
VERSION     DASRVO010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377774"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFF05_06345"
     CDS             complement(<1..281)
                     /locus_tag="VFF05_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZW51460.1"
                     /translation="MRDEPQPVDFAFVLGSPTLSSVEPAIALYKQGLARKIVISGKGP
                     AQARADAPPEALMYRDHAVRSGVAADDVLTEDRAANTLENFRFSRALIER"
     gene            complement(460..552)
                     /locus_tag="VFF05_06350"
     tRNA            complement(460..552)
                     /locus_tag="VFF05_06350"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Ser,seq:gct)
     gene            complement(597..803)
                     /gene="csrA"
                     /locus_tag="VFF05_06355"
     CDS             complement(597..803)
                     /gene="csrA"
                     /locus_tag="VFF05_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HZW51461.1"
                     /translation="MLILTRRVGETLMIGDAVSVTVLGVKGNQVRIGINAPKDVAVHR
                     EEIYQRIKREHDAPGGETPPAAEG"
     gene            complement(961..>1571)
                     /locus_tag="VFF05_06360"
     CDS             complement(961..>1571)
                     /locus_tag="VFF05_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZW51462.1"
                     /translation="GDIGLFKIVSESGVASGIRRIEALTGAGALAFIVDEERRLGEVA
                     QLLSAGGGDAIEKLRQVLDRQKKLERELDATKAKAASAAAGDLAASAQDVAGIKVIAA
                     RLDGADAKSLRDSVDTFKQKLGDCVVLLAGSADGKVALVAGVSGKALGTIKAGELVAH
                     VAGQIGGKGGGRADMAQGGGEDSPKLATALAEVPAWVGARLA"
BASE COUNT          270 a          551 c          460 g          290 t
ORIGIN      
        1 cgctcgatca gcgcccggct gaagcgaaag ttctccagcg tgttggcggc gcggtcttcg
       61 gtgagcacat cgtccgcggc aacgccgctg cgcacggcat gatcgcgata catcagcgcc
      121 tccggcggcg catccgctcg cgcctgcgcc ggtccttttc cggaaatcac gatcttgcgc
      181 gccaaccctt gcttgtacag cgcaatcgcc ggttccacac tggacaatgt ggggctgccc
      241 aggacaaagg caaaatccac cggctgtggt tcgtcgcgca agaacagaaa gcgtgtgatg
      301 gctgcacggc tggccaggat gtcggagccg ctcgcggccg gagcagaatc gagtcgccgc
      361 cggaatatcg aaaatcgcac gcgaaacgcc ccgtcccaat tcatcggtcg ccgcaaacca
      421 ctgctgggca accacgcagg gtgtcccccg ggtctgaact ggcggagagg gagggattcg
      481 aaccctcgat agagcttttg accctatact cccttagcag gggagcccct tcggcctctc
      541 gggcacctct ccgtcaaggg cgcgaaggat agcggctcgc ctgcctgcgg taaagctcaa
      601 ccttcggcgg cgggaggagt ttcgccgccc ggtgcgtcgt gttcgcgctt gatgcgctga
      661 taaatttctt cgcgatgcac ggccacatcc ttgggcgcgt tgatgccgat gcgcacctga
      721 ttgcccttga cgccgagcac cgtcacgctg acggcatcgc cgatcatcaa tgtctcgcct
      781 accctgcgcg tcaaaatcag catgacgatt ctccttcagg ttcgcgccgt cccccggcgc
      841 atgtccattg tccgttgcac cggtagcatg cgctttgagg ataaccgaac gcgcattccg
      901 caccagtgac ccaagtcatg gacgcgtcaa ctcccggcgc cgtcgcatcc tagcagagct
      961 tcaagcaagt cgcgcgccaa cccatgccgg cacctcagcc agcgccgtgg cgagttttgg
     1021 cgaatcctcg ccgccgccct gcgccatgtc ggcgcggcca ccgcctttcc cgccgatttg
     1081 acccgccaca tgcgcgacaa gttcgccagc cttgatcgtg cccaacgcct tgccgctcac
     1141 tccggcgacg agtgcgactt tgccgtccgc actgcccgcc agcagcacga cgcagtcgcc
     1201 gagtttctgc ttgaaggtgt cgacgctgtc gcgcagggat ttcgcatccg cgccgtcgag
     1261 acgcgcggcg atcaccttga taccggcgac atcctgcgcg gacgcagcca gatcgcccgc
     1321 cgccgcactc gccgctttcg ctttggtcgc gtcgagttcg cgttcgagtt ttttctgccg
     1381 atccaggacc tgacgcagct tttcgatcgc atcgccgcca ccggctgaca gcagttgcgc
     1441 aacctcgccg agccgccttt cctcatcgac gatgaaagcc agcgcgcccg cgccagtcag
     1501 cgcctcgatg cggcggatgc ctgaggcaac gccggattcg gacacgatct tgaacaggcc
     1561 gatgtcgccg g
//
LOCUS       DASRVO010000513         1425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_351837, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000513 DASRVO010000000
VERSION     DASRVO010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351837"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFF05_06365"
     CDS             complement(<1..889)
                     /locus_tag="VFF05_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZW51463.1"
                     /translation="MTELKRMLGLRDLVLIVIGTVIGSGIFIVPATTLRETGGDVRVA
                     LLVWLVAGVLSLLGALTYGEMGAANPDAGGLYVYIRDAFGELPAFLYGWTALLVIASG
                     SMATLAVAFSAYLGQLLPLSAWMARVVAVLMIVTTMVINVRGTRQSANVQNWTTGLKV
                     TALVGISVLLLVAGGSGSAHTTTAVSVAQHAHTSFSMFATAMIGVLWAYEGWQYATFA
                     AGETRDPQRTFPRGIIVGTACIIGIYVLANLGYVAALGPERASQSPRIAADAVGAVLG
                     PIAGKVIAAVILVSMFSAAN"
     gene            complement(1011..>1425)
                     /locus_tag="VFF05_06370"
     CDS             complement(1011..>1425)
                     /locus_tag="VFF05_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZW51464.1"
                     /translation="ACGGGAKTPASGDTTAAAPAAAAPAAAAAAGPQTPDPGRKVIIV
                     QLIADEKGSRFEPNEIEAHRGDVIRFTLSQGVHNVDFLPDSNPGAKGLPAPSDMLQLA
                     GQTFDVKVTFPNGKYYFQCDPHVALGMHGHLEVEE"
BASE COUNT          247 a          500 c          450 g          228 t
ORIGIN      
        1 cgttcgccgc actgaacatg gacacgagga tcaccgcggc gatcaccttg cccgcgatgg
       61 gcccgagcac ggcgcctacc gcatcggcgg caatacgcgg actctgcgac gcgcgctccg
      121 ggcccagcgc ggcgacgtag cccagattgg ccagcacgta gatgccgatg atgcaggccg
      181 tgccgacgat gatgccgcgc ggaaacgtgc gctgcggatc tcgcgtttca ccggccgcga
      241 acgtcgcgta ctgccaccct tcgtacgccc acagcacgcc gatcatcgcc gtggcgaaca
      301 tcgagaacga cgtatgcgcg tgctgcgcga cgctcaccgc cgtcgttgtg tgagcactac
      361 cactgccgcc ggctacgaga agcagcacgc tgatccccac gagtgcggtc accttgagtc
      421 cggtcgtcca gttttgcacg ttcgcgctct gccgcgttcc gcggacgttg atcaccatcg
      481 tcgtcacgat catcagcacg gcaaccactc tcgccatcca tgccgagagg ggcagcagtt
      541 gtccgagata ggcgctgaag gcgaccgcca gggtcgccat cgacccgctg gcaatcacca
      601 gcagcgccgt ccaaccgtac agaaacgccg gcagctcgcc gaacgcgtcg cggatgtaca
      661 cgtagaggcc gccggcgtcg ggattcgctg cgcccatttc gccatacgtg agcgcaccga
      721 gcagagacaa cacgccggct acgagccaga cgagaagcgc cacacgcaca tcgccgcccg
      781 tctctcgaag cgtcgtcgcc gggacgatga agataccgga cccgatcacc gtcccgatga
      841 cgatgaggac gaggtcgcgc agcccgagca tccgcttcag ttcagtcacg gttcgccttt
      901 ggcaaacgtg tatgtcgcga tacgtgtcga ttcccgcgag gaagcattgc cttccccgcg
      961 ggccgagtca atccggcggg aacgcgcgcg caagcgcgcg cttccctccc tcactcctct
     1021 acttcgagat gaccatgcat gccaagcgcc acgtgcgggt cgcactggaa gtagtacttg
     1081 ccgttcggga acgtcacctt gacgtcgaac gtctggccgg ccagttgcag catgtcgctc
     1141 ggcgccggca agcccttggc gcccggattc gagtcgggca ggaagtcgac gttgtgcacg
     1201 ccctgcgaca acgtgaagcg gatcacgtcg ccgcgatgcg cctcgatctc gttaggctcg
     1261 aaccggcttc ccttctcgtc ggcgatcagt tggacgatga tcaccttgcg accggggtcc
     1321 ggagtctgcg ggccggccgc ggcggccgca gcgggtgcgg cggccgccgg cgcggccgcc
     1381 gtcgtgtcgc cggacgccgg cgttttggca ccgcctccgc aggcg
//
LOCUS       DASRVO010000514         1047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_92206, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000514 DASRVO010000000
VERSION     DASRVO010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92206"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..629
                     /gene="rplE"
                     /locus_tag="VFF05_06375"
     CDS             75..629
                     /gene="rplE"
                     /locus_tag="VFF05_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZW51465.1"
                     /translation="MAKPRLKERYKAEIAPELMRQFSYANAMQVPRVQKVTVNIGLGE
                     AKENARAVETATADIATITGQKPVVTKAKKAIANFKIRQNMPVGVAVTLRGDRMWEFL
                     DRLLNAALPRIRDFHGVPVKAFDGRGNFSLGVREQLIFPEIDYDKVDRIRGMQINIVT
                     TARTDEEGRRLLEMMGMPFSKERA"
     gene            632..817
                     /locus_tag="VFF05_06380"
     CDS             632..817
                     /locus_tag="VFF05_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZW51466.1"
                     /translation="MAKISMVVKANREPKFSTRKKNRCKLCGRPRAYMRHFALCRICF
                     RQHALKGQLPGVKKSSW"
     gene            899..>1047
                     /gene="rpsH"
                     /locus_tag="VFF05_06385"
     CDS             899..>1047
                     /gene="rpsH"
                     /locus_tag="VFF05_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZW51467.1"
                     /translation="MNMTDPIADMLTRIRNAVTAKHEYVNIPASKMKLAIADVLRGEG
                     FIRDY"
BASE COUNT          218 a          312 c          365 g          152 t
ORIGIN      
        1 gtgcgcgtcg gcttccagag ccgtgcggac ggcaagcacc ggatttgccg cagctgcggc
       61 caggacatcg actgatggca aagcctcgac tgaaggaacg ctacaaggca gagattgcgc
      121 cggagctgat gcggcagttc agctacgcga acgccatgca ggtgccgcgc gtgcagaagg
      181 tgaccgtcaa catcggcctt ggcgaggcga aggagaacgc gcgcgccgtc gagacggcga
      241 cagccgacat cgccaccatc accggacaaa agccggtggt gacgaaggcg aagaaggcca
      301 tcgcgaattt caagatccgg cagaacatgc cggtcggcgt cgcagtgacc ctgcgcggcg
      361 accggatgtg ggagttcctc gaccggctgc tgaacgccgc gctgccgcgc atccgcgact
      421 tccacggcgt gccggtgaag gcgttcgacg gccgcggcaa cttcagcctc ggcgtgcgcg
      481 agcagttgat cttcccggag atcgactacg acaaggtgga ccggatccgc ggcatgcaga
      541 tcaacatcgt cacgacggcg cgcaccgacg aagaagggcg gcgcctgctg gagatgatgg
      601 ggatgccgtt ctcgaaggag cgtgcgtagg catggccaag atctcgatgg tggtcaaggc
      661 gaaccgggag ccgaagtttt cgacccggaa gaagaatcgc tgcaagctgt gcgggcggcc
      721 gcgggcgtac atgcgccact tcgcgctctg ccgcatctgc ttccggcagc acgcgctgaa
      781 ggggcagctg cccggcgtga agaagtcgag ttggtaatcc gggtcgcgtg tgcggccctt
      841 tccgaagctc gatgccgata gcgcatcgag agcgcgggag aacgaggagg cgacagcctt
      901 gaacatgacg gacccaatcg cggacatgct gacgcgcatt cgcaacgccg tgacggcgaa
      961 gcacgagtac gtcaacatcc cggcgtccaa gatgaagctc gcgatcgcgg acgtgctgcg
     1021 gggcgaggga tttatccgcg actacca
//
LOCUS       DASRVO010000515         1239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_169646, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000515 DASRVO010000000
VERSION     DASRVO010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169646"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            487..1065
                     /locus_tag="VFF05_06390"
     CDS             487..1065
                     /locus_tag="VFF05_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51468.1"
                     /translation="MVLRESRGENKQDESLRPFGKVHAKLSDGREIELETSWYQFLGD
                     MHIRLVFDGGQTMQSASPEDLARLHLSPEQALELAVENLKRRYGAPAAQPWSGRLMQV
                     QGGAADLNSSYFLDREFWREMSSQHPEGLVVAVPRRAGLVYAPLSDETAVTSLRFSAA
                     ALYAGGPGARLSSALYLFKDGRWSVFQAPQAH"
BASE COUNT          191 a          401 c          424 g          223 t
ORIGIN      
        1 ccggcgcgct gttggcggcg ggcgtggccg gcacgctggc ggcgggcgtc gacgcgcccg
       61 ccgcgctcgc ggcattcggc tgggtccgct gcgtgtatgc gtccggttcg cgtttgttga
      121 agaaggggga ttgcaatgcc atggtgtact tcctctgagt ggcgttagtg agctgccgat
      181 gctaaggatg acaaggcgct cgcgctcgtc cgtttacgca atatcggtaa cgaatatcgt
      241 tgccgcgtac cgacgcatgc gtcgcgcgcc gcgacttttt tcgcgctgcg cgtaaatggc
      301 tgttgcggcg aaacacacgc cggcgcacga tgactttcgt caatgagggg aatcgcgatg
      361 aagttcaccg ggcgcgtccg agtttttttc gcgctgtttg ccgcgctcgc gtgcgccgca
      421 tccgcactgg ccgcggtcgc cgggccgccc ggagagccga tcgacaccag cgccggcaac
      481 ctgctcgtgg tgctgcgcga atcgcgcggc gagaacaagc aggacgagtc gctgcggccg
      541 ttcggcaagg tgcatgcgaa actcagtgac gggcgcgaga tcgaattgga aaccagctgg
      601 taccagttcc tcggcgacat gcacatccgg ctggtgttcg acggcgggca gacgatgcaa
      661 agcgcttcgc ccgaggacct ggcgcggctg cacctgagcc cggagcaagc cctggaactg
      721 gcagtcgaga acctcaaacg tcgttacggc gcacccgccg cgcagccctg gagcggcagg
      781 ctgatgcagg tgcagggcgg cgcggcggat ttgaacagca gctatttcct cgatcgcgag
      841 ttctggcgcg aaatgtcgtc gcagcacccg gaagggctcg tggtggccgt gccgcgccgc
      901 gccggcctgg tctacgcgcc tttgtcggat gaaacggccg tcacgagcct gcgcttcagc
      961 gccgccgccc tgtatgccgg cgggcccgga gcgcgcctgt cctctgccct gtacctgttc
     1021 aaggacggcc gctggtcggt gttccaggcg ccgcaggcgc actaaccggc gccctcagta
     1081 ggtcagggtg tagcgcgaac cgcggaacct caccacggcc gctttcggct tgatctgctc
     1141 caggatcacc gccggaccga tctcgctgcc ttcgttgaac acctgcccgt tcacgatcag
     1201 catgcgctgg gcggcattcg ccgagtagac gccgccgct
//
LOCUS       DASRVO010000516         1666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_223044, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000516 DASRVO010000000
VERSION     DASRVO010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223044"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..481)
                     /locus_tag="VFF05_06395"
     CDS             complement(5..481)
                     /locus_tag="VFF05_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51469.1"
                     /translation="MRDAPEGFANYCALPNVRTPWELLWHMTGVIGYARTMLRGDTYA
                     PPKLDSFDAEVARFHAMLAELRDAFADPALSARITDEQFLQGPLADAMTHAGQLAMLR
                     RFAGKPVPSEDFIFADVRADNVSRDQPSPRAPDLWWHPDDPPPSPGPDHPSRPRTP"
     gene            complement(730..>1666)
                     /locus_tag="VFF05_06400"
     CDS             complement(730..>1666)
                     /locus_tag="VFF05_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZW51470.1"
                     /translation="GLTNRLPLRDEGYQGPVAVEGRPDLDGVNRPNSLYRIVTPRYFA
                     AMGLRLRDGRGIDSTDVMGSLPVTVISESFARRMWPGQSALGKHIYSGWSGKVVSLTV
                     VGVTREAKLISMTGAVPFVMFVPRAQQASNSGGVLVVRSSTPPTLVMPAVRRAIAAID
                     PQVAVARIETMDDVVAAALAEPLRLRFFLTVFAAIALVLGTIGVYGVVSYAVARRRAE
                     FAIRMALGATPERVVREVAERGLGPVFAGVVIGIAAALALSGLLHRFLYGLGATDPAS
                     LGAAAGVLLGAGIAAALVPAARAGRTSPAEALRAD"
BASE COUNT          281 a          595 c          544 g          246 t
ORIGIN      
        1 ctcgtcaggg agtacgggga cgtgaaggat ggtcgggtcc cggcgacggc ggcggatcat
       61 ccgggtgcca ccagagatcg ggcgcgcgcg ggctcggctg gtcgcggctc acattgtcgg
      121 cccgcacgtc agcgaagatg aaatcctccg acggtaccgg cttgccggcg aaccgccgca
      181 acatcgcgag ctggccggcg tgcgtcatgg cgtcggccaa cgggccttgg agaaactgtt
      241 catccgtgat ccttgccgac agcgccggat cggcgaaagc gtcgcgcagc tccgcgagca
      301 tcgcgtggaa gcgcgccact tcggcgtcga agctgtcgag ctttggcggt gcgtacgtat
      361 ctccacggag catcgtccgg gcgtagccga tcacgcccgt catgtgccag aggagctccc
      421 acggcgtgcg gacgttcggc agcgcgcaat agttcgcgaa accctcgggc gcgtcgcgca
      481 atgccttctg cgtgcgatac gcaagcgccg ccaggcgacc aggccgagtc gatgttttca
      541 gctttacact cctgccgtcg cgctccgatc gccaacgacg gcctaaccgc agggccgagt
      601 tccggccgag cgaaggaaac cgagcccgcg ttctcgctga tcgtcgatgc aacggacagg
      661 aacgttcctg ccctcaaggc gctctgatga cgaggatctg tcgcggcgcg ccttccaaat
      721 cgcattgcgt cagtcggccc gtaacgcctc ggctggactc gtgcgcccgg cgcgcgccgc
      781 cggcacgagg gccgcggcga taccggctcc gagcagcact cctgccgcgg cgccaaggct
      841 ggccgggtct gtcgcgccta acccgtagag aaagcggtgc agcaatccac tgagcgccag
      901 ggcagccgcg atcccgatca cgacgcctgc gaacaccggg ccgagtccgc gttctgccac
      961 ctcgcgtacc acgcgctccg gcgtcgcgcc gagcgccatg cgaatggcga actccgcccg
     1021 ccggcgggcc accgcatacg acaccacgcc gtagacgccg atcgtgccta acacgagcgc
     1081 gatggcggcg aacaccgtga ggaagaagcg gagccggagc ggctcggcga gcgcggcggc
     1141 aaccacgtca tccatcgttt cgatgcgcgc aaccgcgacc tgcggatcga tcgctgcgat
     1201 cgcccggcgc accgccggca tcacgagcgt cggcggcgtc gacgaccgca ccaccagcac
     1261 gcccccggag ttcgacgcct gctgcgcgcg cggcacgaac atcacgaacg gtacggcgcc
     1321 ggtcatgctg atcaacttcg cctcgcgcgt gacgccgacg acagtgagcg acaccacctt
     1381 tcccgaccaa cccgagtaga tgtgcttgcc gagcgcgctc tgacccggcc acatgcgccg
     1441 cgcgaacgat tcgctgatca ccgttacggg caggctgccc atcacgtccg tcgagtcgat
     1501 gcctcgaccg tcacgcagtc tcagccccat ggccgcgaag taccgcggcg tcacgattcg
     1561 gtagaggctg ttcggacggt tcactccatc caggtccggg cggccctcga cggccacggg
     1621 tccctggtac ccttcgtcgc gaagcggcag cctgttcgtt aggcct
//
LOCUS       DASRVO010000517         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_301277, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000517 DASRVO010000000
VERSION     DASRVO010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301277"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1623
                     /gene="parC"
                     /locus_tag="VFF05_06405"
     CDS             <1..>1623
                     /gene="parC"
                     /locus_tag="VFF05_06405"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HZW51471.1"
                     /translation="AERAYLDYSMYVVLDRALPFIGDGLKPVQRRIVYSMSELGLDAG
                     AKPKKSARTVGDVIGKFHPHGDSACYEAMVLMAQPFSYRYPLIDGQGNFGSQDDPKSF
                     AAMRYTESRLTPIAGLLLDEIGLGTVDFTPNFDGTLKEPVWLPARVPHVLLNGATGIA
                     VGMATDIPPHNLREVASACIHLLDEPEVSVAELCRHVKGPDFPTRAEIITPKRDLHAM
                     YATGNGSVRCRAVYAIENGNAVITALPYQVSGEKIIAQIAEQMRAKKLPMLEDVRDES
                     DHENPVRIVLIPRSARVDLDEMMQHLNATTDLERSYRVNLNMIGLDGKPQVKNLKQIL
                     VEWLRFRQQTVTRRLEHRLQKVERRLHLLEGLRIVFLNLDEVIRIVRSEDEPKPVLIA
                     RFKLSEEQADYILETRLRQLARLEEMKLNAERDALEEERARIAVTLKSPAKLKTLIKD
                     ELAADAEKYGDARRAPLVVRDVSQALDEAALVVSEPATVVLSEKGWVRAAKGHDVDAA
                     ALGFREGDKFRNAAKGKTTQNVAFIDSSGRSYS"
BASE COUNT          328 a          537 c          504 g          254 t
ORIGIN      
        1 cgccgagcgc gcttatctcg attattcgat gtacgtggtg ctcgaccgcg ccctgccctt
       61 catcggcgac ggattgaagc cggttcagcg ccgcatcgtg tattcgatga gcgaactggg
      121 cctcgacgcc ggcgccaagc cgaagaaatc cgcgcgcacc gtcggcgacg tcattggcaa
      181 gttccatccg cacggcgaca gcgcctgcta cgaggcaatg gtgctgatgg cgcagccgtt
      241 ctcgtaccgc tatccgttga tcgacggcca gggcaacttc ggctcgcagg acgatcccaa
      301 atccttcgct gcgatgcgct acaccgaatc gcgactgacg cccatcgccg gattgctgct
      361 cgacgaaatc ggcttgggca cggtcgactt cacgccgaac ttcgacggca cgctgaagga
      421 acccgtgtgg ctgccggcgc gcgtgccgca cgtgctgctc aacggtgcga ccggcatcgc
      481 ggtgggcatg gccaccgaca ttccgccgca caatctgcgt gaagtcgcca gcgcctgcat
      541 ccaccttctc gacgaacccg aagtcagcgt cgcggaactt tgccgccacg tgaagggccc
      601 ggattttccg acccgcgccg agatcatcac gcccaagcgc gacctgcacg ccatgtacgc
      661 caccggcaac ggttccgtgc gttgccgcgc ggtgtacgcg atcgagaacg gcaacgccgt
      721 catcaccgcg ctgccgtatc aggtgtccgg cgagaagatc atcgcgcaaa tcgccgagca
      781 gatgcgcgcg aagaagctgc cgatgctcga ggacgtgcgc gacgagtccg accacgagaa
      841 tccggtgcgc atcgtgctga tcccgcgcag cgcgcgcgtg gacctggacg agatgatgca
      901 gcatctgaac gcgaccaccg atctggaacg cagctatcgc gtcaatctca acatgatcgg
      961 cctggacggc aagccgcagg tcaagaacct caagcagatc ctcgtcgaat ggctgcgttt
     1021 ccgccagcag accgtgaccc ggcgcctcga acatcgcctg cagaaggtcg agcgccgcct
     1081 gcacctgctc gaaggcctgc gcatcgtctt tctgaatctg gacgaagtca tccgcatcgt
     1141 gcgcagcgag gacgaaccga aacccgtcct catcgcgcgc ttcaagttga gcgaggaaca
     1201 ggccgattac atcctggaaa cccgcctgcg ccagcttgcg cggctggagg aaatgaaact
     1261 caacgccgaa cgcgatgcgc tggaagaaga acgcgcacgc atcgcggtga cgttgaagtc
     1321 gccggcgaaa ctgaagacgc tgatcaagga cgaactcgcc gccgatgcgg aaaaatacgg
     1381 cgacgcgcgc cgcgcgccgc tggtcgtgcg cgacgtgtcg caggcgctgg acgaggccgc
     1441 actcgtggtc agcgagccgg ccaccgtggt gctgtcggaa aaaggctggg tgcgcgcggc
     1501 caagggccac gacgtcgacg ccgccgcgct cggcttccgg gaaggcgata aattccgcaa
     1561 tgcggcaaaa ggcaagacca cgcagaacgt cgccttcatc gactcgtccg gaaggagcta
     1621 ttc
//
LOCUS       DASRVO010000518         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_301280, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000518 DASRVO010000000
VERSION     DASRVO010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301280"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VFF05_06410"
     CDS             complement(<1..392)
                     /locus_tag="VFF05_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51472.1"
                     /translation="MFWYAEPWTLRYWLWLAASFALSAFVTWAALHYARRRALVDLPG
                     RRRSHAVPTPRGAGIGIVVAALASIFVLAHAEPAAASGLRLLVPIALVAAIGWIDDHR
                     PLPVLVRLGVHCLAVAIFLQPLLAALFAA"
     gene            complement(400..1587)
                     /gene="lapB"
                     /locus_tag="VFF05_06415"
     CDS             complement(400..1587)
                     /gene="lapB"
                     /locus_tag="VFF05_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HZW51473.1"
                     /translation="MSALPPLLLPALAFLLPLAALSGWYYGRRSSERSRRAGVSELSS
                     SYFRGLNYLLNEQPDKAIEVFLKLAEFNRDTVETHLALGNLFRRRGEVDRAIRVHQHL
                     IARPNLNNEEKTVALLELGEDYMRAGLLDRAETLFTDLIAMDAQVPSALKHLISIYQH
                     EKDWDKAIDHARRLEKISGESEGPTIAQFHCELSEQARAARDFSAAHAHLENAFVCQP
                     ECVRAYIVLGHLEMQQGNLEAAAQAFERVATLDVDFVPEVLTPLLDCYARLGQMQRAE
                     RFLVDIIERSQGVSPVIALAKLYSGTRGEAAAIEFLTRQLRQRPSVRGLMTLIGASLD
                     NSRGDARENLLILQDLTRKLIEGQAMYRCSKCGFGAKAHHWQCPSCKTWGSVRPIHGV
                     AGE"
BASE COUNT          312 a          540 c          562 g          263 t
ORIGIN      
        1 gcggcaaaca gcgcggcgag cagcggctgc aggaagatcg ccacggccag gcagtgcacg
       61 ccaaggcgca ccagcaccgg cagcggacga tggtcgtcga tccagccgat tgccgcgacg
      121 agagcgatcg ggacaagcag acgcaggccg gaagcggctg ccggctctgc atgcgccaga
      181 acgaaaatcg acgccagcgc ggcgacgacg atgccgatgc cggcgccgcg cggagtcggc
      241 acggcgtgcg aacgccggcg ccccggcaga tcgacgaggg cgcgtcgacg cgcgtagtgc
      301 agcgcagccc aggtcacgaa cgcagacagc gcgaaactgg cggcaagcca gagccagtag
      361 cgcagggtcc agggttcggc ataccagaac atgccggtgt cactcgccgg ccacgccgtg
      421 gattggtcgc accgatcccc aggtcttgca gctcgggcat tgccagtgat gcgccttggc
      481 gccgaaaccg cacttgctgc agcggtacat ggcctggcct tcgatcagct tgcgggtcag
      541 atcctgcaga atcagcaggt tctcgcgcgc atcgccgcgc gaattgtcga gcgacgcgcc
      601 aatcagcgtc atcagtccac gcacggacgg acgctggcgc aattgccggg tcaggaattc
      661 gatggccgcc gcctcgccac gcgttccgga gtagagcttg gccagcgcga tcaccggcga
      721 cacgccctgc gagcgctcga tgatgtcgac gagaaaacgt tccgcgcgct gcatctgccc
      781 gagccgcgca tagcagtcga gcagcggcgt gagtacttcg ggaacgaaat ccacatccag
      841 cgtggccacg cgctcgaacg cctgcgcggc ggcttcgagg ttgccctgtt gcatttccag
      901 atgaccgagc acgatgtagg cgcgcacgca ttccggctgg caaacgaacg cattttccag
      961 gtgcgcatgc gctgcggaaa aatcgcgcgc ggcgcgcgcc tgctcggaca gctcgcaatg
     1021 gaactgcgca atggtcgggc cttcggattc gccgctgatc ttttccaggc ggcgcgcgtg
     1081 gtcgatggcc ttgtcccagt ccttttcgtg ctgatagatg ctgatcaggt gcttgagcgc
     1141 cgacggcacc tgcgcgtcca tcgcgatcag gtcggtgaac agcgtttcgg cacgatcgag
     1201 caggccggcg cgcatgtagt cctcgccgag ctcaagcaag gcgacggtct tttcctcatt
     1261 gttgaggttc ggccgcgcga tcagatgctg gtgcacgcgg atcgcccgat cgacttcgcc
     1321 gcgacgccga aacaggttgc cgagcgcgag gtgggtttcc accgtgtcgc ggttgaattc
     1381 ggcaagcttg aggaagacct cgatggcctt gtcgggctgt tcgttgagca ggtaattcag
     1441 gccgcgaaaa taactcgacg acaattcgct cacgccggct cgccgcgaac gttcgctgct
     1501 gcgacgcccg taataccagc cggacaacgc ggccaacggg agcaggaacg cgagcgccgg
     1561 cagcaacagc ggcggcaggg cactcatgct tgacggactc aggccggccg gcgcacggcg
     1621 acgttgaccc gctcgcgcaa ttccttgccg ggcttgaagt gcggcacatg cttgccg
//
LOCUS       DASRVO010000519         1948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_197109, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000519 DASRVO010000000
VERSION     DASRVO010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197109"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..595
                     /gene="rfbC"
                     /locus_tag="VFF05_06420"
     CDS             38..595
                     /gene="rfbC"
                     /locus_tag="VFF05_06420"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HZW51474.1"
                     /translation="MKIIETILPGCVVIEPQVHGDARGFFYESFHADKYKNAGLDLHF
                     VQSNVSRSARGVLRGLHYQWPNPQGKLVSVLEGEVYDVAVDIRVDSPTFGRWAAAMLT
                     ADNKRQFWIPEGFAHGFAVVSDNATFCYQCTALYDRAADAGIRWNDADIAIDWPLSTP
                     LLSDKDANAPFLRDMPREKLPKYTK"
     gene            592..1512
                     /gene="rfbD"
                     /locus_tag="VFF05_06425"
     CDS             592..1512
                     /gene="rfbD"
                     /locus_tag="VFF05_06425"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HZW51475.1"
                     /translation="MKILLLGANGQVGFELARSFAPLGELVCATRSGTLAGGAACLAA
                     DLAQAESLVAILGAVRPDVIVNAAAYTAVDRAEDEADLAQRINAQALAEIGAHAASHN
                     ALVVHYSTDYVFDGAGSRPYRENDATAPLGVYGRSKLDGENALRASGCDHLILRTAWV
                     YAARGHNFLRTMLRLARERDELRIVGDQRGAPTPARLISAATATTLARLLARRAGERD
                     KRLGTYHLSASGQCTWFDFAEAIFARARTAGLIERVPRLVPIATSDYPTPARRPAYSV
                     LDCSKIDDAFGIFLPPWQDGLNNVIGELAG"
     gene            1522..>1948
                     /locus_tag="VFF05_06430"
     CDS             1522..>1948
                     /locus_tag="VFF05_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZW51476.1"
                     /translation="MSQLVPVVLSGGAGTRLWPVSRSAYPKPFMRMADGQSLLYKTLD
                     RALHVSGAGPVLTVTGRDYYFLTRDEYARHPGAVARALPFLLEPVGRNTAPAIALAAM
                     YTMEYCSADAVLLVLPADHLIGDVDAFAADARRAHALARD"
BASE COUNT          320 a          629 c          642 g          357 t
ORIGIN      
        1 aatatctgca gcagctggtg aagcagggtc cggtggcatg aagatcatcg agacgatcct
       61 gcccggctgc gtggtgatcg agccgcaagt gcacggcgat gcgcgcggat ttttctacga
      121 aagcttccat gcggataagt ataaaaatgc cgggctcgac ctgcacttcg tgcagagcaa
      181 cgtgtcgcgt tccgcacgcg gcgtgttgcg cggactgcac taccagtggc cgaatccgca
      241 gggcaagctg gtgagcgtgc tcgaaggcga ggtctacgat gtcgccgtgg acatccgcgt
      301 cgattcgccc acgttcgggc gctgggccgc ggccatgctc accgccgaca acaagcgcca
      361 gttctggata ccggaaggct tcgcccatgg tttcgccgtg gtatcggaca acgccacgtt
      421 ctgttaccag tgcacggcgt tgtacgaccg cgccgcggat gccggcatcc gctggaacga
      481 cgccgacatc gccatcgact ggccgctgtc gacgccgttg ctgtcggaca aggacgcgaa
      541 tgcgccgttc ctgcgcgaca tgccgcgcga gaaattgccg aagtatacaa aatgaaaatc
      601 ctgctgctcg gcgcgaacgg acaggtcggc ttcgagttgg cacgctcgtt tgcgccgctt
      661 ggcgaactcg tctgcgcgac gcgctccgga acattggcgg gcggcgctgc gtgcctggct
      721 gccgatctcg cccaggccga gtcgcttgtg gccatcctgg gtgcggtcag gccggatgtg
      781 atcgtcaacg cggcggcgta taccgccgtc gaccgtgccg aggacgaagc ggatctcgcg
      841 caacgcatca atgcccaggc gctcgcggaa atcggcgcgc acgcggcatc gcacaacgcg
      901 ctggtcgtgc actattcgac ggattacgtg ttcgacggcg ccggttcgcg gccttaccgc
      961 gagaacgacg caaccgcgcc gctcggcgtc tacggacgca gcaagctgga cggcgagaat
     1021 gccttgcgcg cttcgggttg cgatcacctg atcctgcgca ccgcctgggt gtacgccgcg
     1081 cgtgggcaca acttcctgcg cacgatgctg cgtctggcgc gtgagcgcga cgaattgcgc
     1141 atcgtcggcg atcagcgcgg cgcgccgacg ccggcacgcc tgatttctgc ggcgacggcg
     1201 acgacattgg cgcgcctgct ggcacggcgt gcgggcgagc gcgacaagcg actgggcact
     1261 tatcatctta gcgcctcggg gcagtgcacc tggttcgatt tcgccgaagc gattttcgcg
     1321 cgtgcgcgga cggccggatt gatcgaacgc gtgccacgcc tcgtgccgat cgcgacgtcc
     1381 gactatccga cgcccgcgcg ccgaccggcc tattccgtgc tcgattgcag caagatcgac
     1441 gatgcgttcg gcatcttcct gccgccgtgg caggatggtt tgaacaacgt gatcggcgaa
     1501 cttgccggtt aggatgaaac catgagccaa ctcgttcccg tcgttctctc cggcggcgcc
     1561 ggcacccgat tgtggccggt gtcgcgcagt gcgtatccga aaccattcat gcgcatggcc
     1621 gacgggcagt cgctgctgta caagaccctg gatcgcgccc tgcatgtgtc cggcgcaggc
     1681 ccggtgctga ccgtgaccgg ccgtgattac tatttcctga cccgcgacga atacgcgcgt
     1741 catccgggcg ccgtcgcacg ggcgttgccg tttctgctcg aacccgtcgg tcgcaacacc
     1801 gcgccggcca tcgcgcttgc ggcaatgtat acaatggaat attgcagcgc ggatgcggtg
     1861 ttgttggtgt tgccggccga tcacctgatc ggcgacgtcg atgccttcgc cgccgatgcg
     1921 cgtcgcgcgc acgcgctggc gcgcgacc
//
LOCUS       DASRVO010000520         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_144292, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000520 DASRVO010000000
VERSION     DASRVO010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144292"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VFF05_06435"
     CDS             <1..358
                     /locus_tag="VFF05_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HZW51477.1"
                     /translation="QHWRHGARWFWDTLVDADLANNTLGWQWVAGCGADAAPYFRVFN
                     PHTQAAKFDPEAIYLKRWLPELAALPAKLLLEPWRDPAALAMCGYPVPTVDVSATRQA
                     ALAAWQELATARIGAS"
     gene            complement(425..970)
                     /locus_tag="VFF05_06440"
     CDS             complement(425..970)
                     /locus_tag="VFF05_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51478.1"
                     /translation="MTDRDLKNHDLKNLFRQFAGARGTMPVPDVEDLLALSRLPHAED
                     AASPLHADLLRFSRELEPVSAQLGTDLAAALGETNAVVAHRLASPRRAASAPRRWRAA
                     VASAMAASLIVAVALFATHRSHAPAPESAAAHALPDRIFAGFDERNVATGEKAGQGDQ
                     IFRGNFVPDEIFNSSRHRNDG"
     gene            complement(963..>1567)
                     /locus_tag="VFF05_06445"
     CDS             complement(963..>1567)
                     /locus_tag="VFF05_06445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZW51479.1"
                     /translation="FRAERLDISRSARGQCAMSFDPALRARLHQLLADYGARVRGLIE
                     GHGIAQYGIDPADIEQEVRIRLWRALERDRNAAFHASYIHRTVLSAVIDAIRAASVRA
                     AEPLPEDESAGVEALLAVSPGPERLAGGAQDFSRVAACMAGLPLRRREAVALHLQGFS
                     LREIGGAVGVSEEAARKLLDRGMQTLRERLAAQGFGEFDD"
BASE COUNT          279 a          534 c          513 g          241 t
ORIGIN      
        1 ccagcactgg cggcacggcg cacgctggtt ctgggacacg ctggtcgacg ccgacctcgc
       61 caacaacacg ctcggatggc aatgggtcgc cggttgcggc gccgacgccg cgccttactt
      121 tcgcgtgttc aatccccaca cccaggccgc caagttcgat cccgaggcga tttacctcaa
      181 gcgctggctg cccgagctgg cggcattgcc ggccaaattg ctgctcgagc catggcgcga
      241 tcccgcggca ctcgccatgt gcggataccc tgtgccgaca gtcgatgtgt cagcaacgcg
      301 gcaggccgcg ctcgcggcgt ggcaggaact cgccacggca agaatcgggg cgagttgacc
      361 tcgaccggca ccctctgaca tccgacccgc cgaggccgat gacaggcgga gcttccggaa
      421 acgctcagcc gtcgttgcga tgcctggacg aattgaaaat ttcgtccggc acgaagttgc
      481 cgcgaaagat ctggtcgcct tgcccggcct tctcgccggt cgcgacgttg cgctcgtcga
      541 agccggcaaa gatacgatcc ggcagtgcgt gcgcggcggc cgattccggc gcgggcgcat
      601 gactacgatg cgtggcaaac aaagcgacgg caacgatgag gctggcagcc attgcggaag
      661 ctacggcagc acgccagcgg cgcggcgcgg acgcggcacg gcgaggcgag gcgaggcgat
      721 gcgcgacgac ggcgttcgtt tcgccgagcg cggcagccag gtcggtaccc aattgcgccg
      781 aaaccggttc gagttctcgg ctgaagcgca gcaggtcggc atgcaacggc gaagccgcgt
      841 cttcggcatg cggcaggcgc gacagggcca gcaaatcctc gacgtccgga acgggcatcg
      901 tgccgcgtgc tccggcgaac tggcggaaca ggtttttcaa atcatggttt ttcaaatcgc
      961 gatcagtcat cgaactctcc aaaaccctgc gcggcaaggc gctcgcgcaa cgtctgcatg
     1021 ccgcggtcga gcaatttgcg cgccgcttcc tccgaaacac ccaccgcgcc gccgatctcg
     1081 cgcaggctga agccttgcag atgcagggct accgcctcgc gccgtcgcag cggcaatccg
     1141 gccatgcagg cggcgacgcg ggaaaaatcc tgtgctccac cagcgaggcg ctccggcccc
     1201 ggcgacacgg ccagcaaggc ctcgacgccg gcgctttcgt cctccggcag cggttccgcc
     1261 gcgcgcacgc tggccgcacg gatggcgtcg atcaccgccg aaagcactgt cctgtggata
     1321 taagacgcat gaaaggccgc attccggtca cgttccagcg cccgccacag ccggatgcgg
     1381 acctcctgct cgatatccgc cggatcgatg ccgtattgcg cgatgccgtg gccctcgatc
     1441 aggccgcgca cgcgcgcgcc gtaatcggcc agaagctggt gcaggcgcgc gcgcagggcg
     1501 gggtcgaaac tcatggcgca ttgtcccctt gcgctgcgcg agatgtcgag tcgttcagct
     1561 cgaaata
//
LOCUS       DASRVO010000521         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_223137, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000521 DASRVO010000000
VERSION     DASRVO010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223137"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..795
                     /gene="fabG"
                     /locus_tag="VFF05_06450"
     CDS             52..795
                     /gene="fabG"
                     /locus_tag="VFF05_06450"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZW51480.1"
                     /translation="MNDLLKGEIALVTGASRGIGAAIADTLAAQGAKVIGTATSEAGA
                     QAIGQRLAAHGGVGRVLDVTDGAAVETLVDDVAKEFGSVTILVNNAGITRDQLLMRMK
                     DEDWQVILDTNLSSVYRTSKAVMRGMMKARKGRIVSIASVVGVTGNPGQANYAAAKAG
                     IIAFSKSLAREIGSRGITVNVVAPGFIDTDMTRALTDDQRKALLPNIALGKLGVAQDI
                     ADAVLFLASPSANYITGETLHVNGGMYMP"
     gene            894..1133
                     /gene="acpP"
                     /locus_tag="VFF05_06455"
     CDS             894..1133
                     /gene="acpP"
                     /locus_tag="VFF05_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZW51481.1"
                     /translation="MSSIEDRVKKIVVEQLGVKEDEVTPNASFVDDLGADSLDTVELV
                     MALEEEFECEIPDEEAEKITTVQQAVDYIKAHVKA"
     gene            1257..2495
                     /gene="fabF"
                     /locus_tag="VFF05_06460"
     CDS             1257..2495
                     /gene="fabF"
                     /locus_tag="VFF05_06460"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZW51482.1"
                     /translation="MSKRRVVVTGLGIVSPVGNDVATAWKNILAGHSGIGPVTHFDAS
                     TFPTRIAGEVRGFDPAQFVAAKDVKKMDPFIHYGVAASLEALKDAGMHPHEHDEERIG
                     CAVGAGIGGIHTIERTSIAYHEGGQRKISPFFVPASIINMASGNLSIMLGLKGPNIAC
                     VTACTTATHNLGLAMRMIQYGDADVMVAGGAEYSTTGVAMGGFCSARAMSTRNDEPTK
                     ASRPWDKDRDGFVLADGAGVLVLEEYEHARKRGARMYCELAGFGMSADAYHITAPSEN
                     GEGAARCMAAALKDAALNVDQIQYVNAHGTSTPAGDAAEAQAVKTCFGEAAKKVMVSS
                     TKSMTGHLLGAAGGVEAIFCVLALRDNVAPPTINLDNPGEGCDLDFVPHTARQTRIDA
                     ALSNSFGFGGTNGTLVFRRL"
BASE COUNT          521 a          833 c          838 g          446 t
ORIGIN      
        1 cggaggagat ccagagatgg caacatgcaa acgaattgaa atggtgcgcg catgaatgac
       61 ttgctaaaag gcgaaatcgc cctcgtcacc ggcgccagcc gcggcatcgg cgcagccatc
      121 gccgacacgc tcgccgcgca aggcgcgaaa gtgatcggca cggcgactag cgaagccggc
      181 gcgcaggcca tcggccagcg gcttgccgcg cacggtggag tcggtcgcgt tctcgatgtc
      241 accgatggcg cggcggtcga aacgctggtc gacgacgtcg ccaaggaatt cggaagcgta
      301 accattctcg tcaacaacgc cggcatcacg cgcgaccagt tgctgatgcg aatgaaggac
      361 gaggactggc aggtcatcct cgacaccaat ctgtcgtccg tttatcgcac ctcgaaagcg
      421 gtgatgcgcg gcatgatgaa ggcacgcaag ggccgcatcg tcagcatcgc ctccgtcgtc
      481 ggcgtgaccg gcaatccggg gcaggcgaat tatgcggcgg ccaaggccgg catcatcgcg
      541 ttctccaaat cgttggcgcg cgagatcggt tcgcgcggca tcaccgtgaa tgtcgtggcg
      601 cccggtttca tcgacaccga catgacgcgc gcgctgaccg acgatcagcg caaggcgctg
      661 ctgccgaaca tcgcgctcgg caagcttggc gtcgcgcagg acatcgccga cgcggtgctg
      721 ttcctcgctt cgccgtccgc gaattacata accggggaaa cattgcacgt gaatggcggg
      781 atgtacatgc cctaggttct tcaaacgctc gttcgctggc gcgccggcaa ggcttccact
      841 acaatccgcg cgcttccgct attggattcc acttttctgg tgagggtttg cacatgagca
      901 gcattgaaga tcgcgtcaag aagatcgttg tcgaacagtt gggcgtcaag gaagatgaag
      961 tcacgccgaa cgcttcgttc gtggatgatc tgggcgcgga ctcgctcgac acggtcgagc
     1021 tggtgatggc gctggaagag gaattcgagt gcgagattcc cgacgaggaa gccgagaaaa
     1081 tcaccaccgt gcagcaggcg gtcgattaca tcaaggcgca cgtcaaggcc tgattttgcc
     1141 acaaggattt ccccgaactg cgcccgtgtg gcgcagtttg catttatgaa aagtgcggcc
     1201 aggacggccg cgttgcgatt ctcgcgatca gggatgagcg caaaggcaaa tctctcatgt
     1261 ccaaacgtcg cgtcgtagtc acaggcctcg gcatcgtcag tcccgtcggc aacgacgtgg
     1321 cgacggcgtg gaagaacatc ctcgccggac acagcggcat cggcccggtc acgcatttcg
     1381 acgcgtcgac gttcccgacc cgcatcgccg gcgaagtgcg cggtttcgac ccggcgcagt
     1441 tcgtcgcggc gaaagacgtc aagaagatgg atccgttcat ccattacggc gtcgcggcgt
     1501 cactcgaggc gctcaaggat gccggcatgc atccgcacga gcacgacgag gaacgtatcg
     1561 gctgtgcggt cggcgccggc atcggcggca tccacaccat cgagcgcacc agcatcgcct
     1621 atcatgaagg cggccagcgc aagatttccc cgttcttcgt gcccgcatcc atcatcaaca
     1681 tggcttcggg caatctttcg atcatgctgg gactcaaggg tccgaacatc gcctgcgtca
     1741 ccgcctgcac cacggccacg cacaatcttg gcctggccat gcgcatgatc cagtacggcg
     1801 atgcggacgt gatggtcgcc ggcggtgcgg agtattcgac gaccggtgtg gcgatgggcg
     1861 gattttgctc ggcgcgcgcc atgtccacgc gcaacgacga gccgaccaag gcgagccgtc
     1921 catgggacaa ggatcgcgac ggcttcgtgc ttgccgacgg tgccggcgtg ctggtactcg
     1981 aagaatacga acacgccagg aaacgcggcg cgcgcatgta ctgcgaactc gcgggattcg
     2041 gcatgagcgc cgacgcctac cacatcaccg cgccgagcga gaacggcgaa ggcgcggcac
     2101 gctgcatggc cgccgcgctc aaggacgccg cactcaacgt ggatcaaatc cagtacgtca
     2161 acgcgcacgg cacgtccacg cccgcgggcg acgcggccga ggcgcaggca gtcaagacct
     2221 gcttcggcga agcggcaaaa aaagtcatgg tgtcgtcgac caagtcgatg accgggcacc
     2281 tgctcggcgc ggccggcggc gtcgaggcga tcttctgcgt gctcgcgctc cgcgacaacg
     2341 tcgcgccgcc gacgatcaac ctcgacaatc cgggcgaagg ttgcgacctg gatttcgtcc
     2401 cgcacaccgc gcgccagacg cgcatcgacg cggcgctgtc aaattcgttc ggctttggcg
     2461 gtaccaacgg aacgctggta tttcgcaggc tttgagtagc ggcgatgtga aggaccctgc
     2521 cgcgcggcgc gctctgaccg gtgcgcgcga tttgctgcca ctcgccgccg cctatccgca
     2581 gcgttatccc tgcttgctcg aaagcgcggc gccctcggcg cagtcgcgct acgacatc
//
LOCUS       DASRVO010000522         1402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_248645, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000522 DASRVO010000000
VERSION     DASRVO010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248645"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..704)
                     /locus_tag="VFF05_06465"
     CDS             complement(69..704)
                     /locus_tag="VFF05_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51483.1"
                     /translation="MTALEDHAATQTEGTIEAKGLTQKKEALRTKLLRDHMAKIARIA
                     RADLPNTPELSHLRMPRGKPTVERLKAAADGMARAAEPHAAVFIAAGMAADFIAQLSA
                     AADALTTAVDDRTQIRTRSRGATVGLKAKLSEGRKVVSILDSFVQTALKDNAALLAQW
                     NSVKRVPRLGGRSQGSASGTSGTSATTGSAATTSTASTTASNAPAPSGAAA"
     gene            1171..>1402
                     /locus_tag="VFF05_06470"
     CDS             1171..>1402
                     /locus_tag="VFF05_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51484.1"
                     /translation="MRVRAVRLDSGAVAIVVGLVHVVGSDVQLENGVGARMRRSIDRW
                     RRDAGACRAALVHATVMGLRVADVGTAGRSRGH"
BASE COUNT          198 a          466 c          471 g          267 t
ORIGIN      
        1 ggagaagccg ctgccccggc gcaccaccca gacgttcggt ccacgttctg gcatcgctcc
       61 ctcccgggtc atgccgccgc gccgctcggc gccggcgcat tgctggccgt cgtcgaagcg
      121 gtggacgtgg tggcggcact accggtagta gcggacgtgc cggacgtgcc ggacgcagag
      181 ccctgcgacc gaccaccgag ccgaggcacg cgcttaacgc tgttccactg cgcgagcagc
      241 gcggcgttgt ccttcaacgc ggtctgtacg aacgagtcga gaatcgagac caccttccga
      301 ccttcggaca gcttcgcctt gagcccaacc gtggcgccgc gcgaacgagt gcggatctgc
      361 gtccggtcgt ccaccgcggt cgtcagcgcg tcagccgccg cggagagctg cgcgatgaag
      421 tcggcggcca ttcccgcggc aatgaacacg gcggcgtgcg gctccgcggc gcgcgccata
      481 ccgtcggccg ccgccttgag gcgctccacc gtcggcttgc ctctcggcat gcggagatgt
      541 gacaactccg gcgtgtttgg cagatccgcc ctggcgatgc gggcgatctt cgccatgtgg
      601 tcgcgcagca acttcgtgcg cagcgcttcc ttcttctgcg tgaggccctt cgcctcgatg
      661 gtgccctcgg tctgcgtggc cgcatggtct tcgagcgcgg tcatggcgtc gaccaaccgc
      721 tgcttcgcgc cggtaccgat gaccgatggc cgagctggtc cacgtggtcc ccgatgaagg
      781 tctccaccgc tcgaagggtc tgaaacatcg tcccctgatc agtacgcata cttcgctttt
      841 cctacctcct tatggatgtg tttgtgagta ctgctaccgt tgacgcacct ccgttgaccg
      901 atccgacgtg tgcgtcgaac gtcggaccgg tgaactgccg gactgctgca actgccgatg
      961 cgttgaatgc cagtgcctcc cgcgttgatg gtgatgcgag ctcgctgctg cgatgcacga
     1021 gtggttgccg tgtcgcgaca cgtcgatgaa gctctcgcgc ccttctactt gtataaatga
     1081 agtaatccgc ccttttggcc ctcccacgtg gcatcgagac gcggagcgcg agcgttcgag
     1141 gcgctttttt tcggtacgag tgcggcggcg atgcgcgtcc gcgcggtgcg cctcgacagt
     1201 ggcgcggtgg cgatcgtcgt cgggctggtg cacgtggtcg gcagcgacgt gcaactcgag
     1261 aacggcgtag gcgcgcgcat gcgccgctcg atcgaccgat ggaggcgaga cgctggtgca
     1321 tgccgcgccg cgctggtgca cgcgaccgtc atggggctgc gcgtcgccga tgtgggtacg
     1381 gccggtcgca gtcgcggcca tg
//
LOCUS       DASRVO010000523         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_301506, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000523 DASRVO010000000
VERSION     DASRVO010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301506"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFF05_06475"
     CDS             <1..498
                     /locus_tag="VFF05_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HZW51485.1"
                     /translation="FKCLLPALLQVEDRMSMAHGLESRVPLLDHPLVEFLATVPADIK
                     FSGGRMKHLLKLAYRDTLPAAIADRRDKMGFPVPLAEWFAAPLRPFVNDVFHSRSARE
                     RPFMNSQAVLANMTGAGRFSRKTWGLLSLELWQRLFHDRAAEYRSLLSAASDTPAAFD
                     AVAAN"
     gene            542..>1015
                     /locus_tag="VFF05_06480"
     CDS             542..>1015
                     /locus_tag="VFF05_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZW51486.1"
                     /translation="MKVLVTGGSGLVGSTVIDQLLARGDEVVAIDNFATGRRDNLTPH
                     PALRLVEGSIVDGRMVDELVGGLRPDVVVHTAASYKDPDDWQSDAMTNCAGSANVARA
                     CRDHKVSRLIYFQTALCYGTRPREQPITLAHPIDPVNSSYAISKTAGEHYIQYSGV"
BASE COUNT          186 a          332 c          317 g          180 t
ORIGIN      
        1 ttcaaatgcc tgctgccggc gctgctgcag gtcgaagatc gcatgagcat ggcgcacgga
       61 ctggagagcc gggttccgct gctcgaccac ccgctcgtcg aatttctcgc caccgtgccg
      121 gccgacatca agttcagcgg cggccgcatg aagcatctgt tgaaactggc gtatcgcgac
      181 acgttgcctg ccgcgatcgc cgatcggcgc gacaagatgg ggtttccggt gccgctggcg
      241 gaatggttcg cagcgccgct gcgcccgttt gtcaacgacg tgtttcacag ccgcagcgcg
      301 cgcgaacggc cgtttatgaa ttcgcaggcg gtccttgcca acatgaccgg ggccggccgc
      361 ttctcgcgca agacttgggg gctcttgagc ctcgaattat ggcagcgatt gtttcacgat
      421 cgcgccgccg aataccgctc gctattgtcg gcagcgtccg atacgccggc agcgttcgat
      481 gcggtcgcgg caaactgaaa cgtcacaacc gcagtcgaca aattatcggg agacgagttg
      541 aatgaaggtg ctcgtgacag gcgggtcggg cctcgtcggc tcgaccgtca tcgaccagtt
      601 gctggcgcgc ggcgacgagg tcgtcgcaat cgacaatttc gccaccggcc ggcgcgacaa
      661 tctgacgccg catccggcac tgcgtctggt ggaaggaagc attgtcgacg gccgaatggt
      721 cgacgaactg gtcggtgggc tgcgccccga tgtcgtcgtc cataccgccg cgtcctataa
      781 agacccggac gattggcagt ctgacgcgat gaccaactgt gccggcagcg ccaacgtggc
      841 gcgcgcctgc cgcgaccaca aggtctcccg gctgatctac ttccagaccg ccctgtgcta
      901 cgggacccgc ccgcgcgagc agccgatcac gctcgcgcac ccgatcgatc cggtcaacag
      961 cagctacgcc atctcaaaga cggccggcga gcactatatc caatacagcg gcgtc
//
LOCUS       DASRVO010000524        11123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_67221, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000524 DASRVO010000000
VERSION     DASRVO010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67221"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFF05_06485"
     CDS             <1..228
                     /locus_tag="VFF05_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HZW51487.1"
                     /translation="DLATPAEHDLLDVDAALDRLDAINPDLRELVELRIFAGMTLEEL
                     AQSSGRSLRTVNRDWQRARALLMTQMAQAVS"
     gene            228..2822
                     /locus_tag="VFF05_06490"
     CDS             228..2822
                     /locus_tag="VFF05_06490"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZW51488.1"
                     /translation="MDAQERDQWRAADQLFQRLIELDDAERRRVLETVQVDAGVHAKL
                     AQLLDSSQRSHRILDGDALNLPWHAAGPVPPNADGDRLLGRCIGNWKLVEAIGRGGMG
                     VVYRAERVGADYDQRAAIKLLAIALHADAEVQRFRRERRILAQLQHPHVAGLIDAGVA
                     EDGTPYLAMALIDGVRIDGWCERQGASLRARVELMLQVCDAVAYAHRQLIVHRDIKPG
                     NVLVDAKGQATLLDFGIARLLDADDTGEQTVTRAFTPDYAAPEQRRGERALGTAVDVY
                     GLGAVLYRLLAGAAPACDAKGEVAPASRVARERGDARGAALLRGDLDAILAQALAEVP
                     TRRYASVDALANDLQAWLHHRPLRAQRATVWTRLRKFVRRNRAASVLALIAILAAGAG
                     FASFILSHRALERRAAQLQAVTKFQTDMLMQFAPDRAGASLHKAVADALGKTVPAANT
                     AATVLNRIDYTGLSIDMLDEAILAPSIDAAHQRFSDQPRVKGMLLLALTEAYLGLGRF
                     DKARALQEQVTALARERFGAEDRLTLMSLRDEMKWVRQAKSPDGEAISRDIVHQHERY
                     LGVNDIDTAIARGLLGEWLMQHGKTTEAESILRDSGTRIARERGQDDPDAVSARANLA
                     FAISSQGRYAESVPLYRSAAESMVKVFGADHPYTLTSLNNLAYALNHSGHGDEALPIY
                     RRVFEARRRVLGAKHRATLISENNLAVGLGRQGQNAEAEPLQREVYAGFRDTLGAHDA
                     TTLHAGLNLARNELALRHHADVDALVAELLEGWQQPRDAKPIAQAQRILAQSLQGQGK
                     YEAAATAFEQSWQAATIANDAKEQRETAQAALAPGDKANASRHSLWQQRSQAVASQPE
                     KPSAASDQ"
     gene            2947..4842
                     /locus_tag="VFF05_06495"
     CDS             2947..4842
                     /locus_tag="VFF05_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51489.1"
                     /translation="MRALPKAFFALIGVAMLTATANAATYRIGSGCAYADLGAAMTYA
                     AAHDFDSQVVFQLRTGTEFSGVYGPNAGYASIEVIDPRWDMVIEGGFDDCLAAVPTGY
                     TTLKTSRAADGMLLVENGLLARHTVAIRRLHFVDASDTQAVFFWDNVTAMLGTGIEVS
                     NNGGGINLAAQVGASSANWPQLTLGDGARVHDNLGSGIAGLGTVTLAGASIDHNQAEN
                     GAGVHLFEPGSRLILDSNGGDNSITENVASAAGGGIYLDHASLLTVAAVSDVNSISLN
                     QAGTSNNLGFGYGGGIYSDGGDIEFHSGAAHAGQHNILDGNSANYGGAIAVVGANAGA
                     GGTYTLVSLLNTRFSYNQAYVRGGALYSRNAVDWTIDRGNLSGCDADFFAPCSVFANN
                     SARNADRAYDPDTPQPNGGVIYLTDERSDGYSRGIARFYHTYFANNSDDQGSAAVAAA
                     TSSAELVFQRDIFAGNAAYVLDVGANLIDSRNTKNLYFLYNTVLPTNYAYYVLHTLGG
                     TADLTGSIIETPELLGVPVPIWYGEGSAQVVHRGCLVSLSALIDANGRAGWVGDARIS
                     ATDFAPAGGSPAIDHCAFTPPIDFYGFAGAVDTKGVPARFGNYDLGAVEQRDIIFYND
                     FGIRPNG"
     gene            complement(4903..5655)
                     /locus_tag="VFF05_06500"
     CDS             complement(4903..5655)
                     /locus_tag="VFF05_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZW51490.1"
                     /translation="MTAKTILVTGASAGFGAATARRFAQAGWRVVACARRGDRLQHLT
                     DEFGASVHTAAFDVRDAAAMQTALDALPAEFRGIDMLVNNAGLALGTAKAQHCDLAQW
                     RQMIDTNITALATLTHKLLPTLIERRGSIVNVSSVAGSYPYTAGNVYGGTKAFVTQFS
                     LGLRCDLHGSGVRVTSVEPGMAETEFTLVRNGGDQVASDKLYQGAHPITGHDIAETIW
                     WVANLPAHLNINRIEVMPVSQSFAGFQIARDN"
     gene            5821..7350
                     /locus_tag="VFF05_06505"
     CDS             5821..7350
                     /locus_tag="VFF05_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZW51491.1"
                     /translation="MRGDLAFRSVILVAGTLLVAISAPAADWMQFGFDPAHSGNNPAE
                     TTLSASATGEFALTALLPIYTVTLPDIADGAPAFLSAAATTNGSRDLLFLETKDGHAL
                     AIDAATGALVWSHATNPSPPPAGQDYTTSSPAIDPNRQYVYFYGLDGYVHKYAVGTGL
                     ETTSGGWPELTTNKPDVEKGSSALAIATAKSGTTYLYVTNGGYPGDAGDYQGHVTVIN
                     LATGAQNVFNANCSDQTVHFVENGTPDCAQVQTAIWARPGVIYSSATDHVYMSTGNGT
                     FDANAGGHDWGDSVFALNPDGTGSGGNPIDSYTPTNFLTLDSSDADLGSTAPAILPST
                     LAGYPDLAVQGGKDQKLRLLNLDNLSSSATVGPGHTGGELQLINVPQGGLVFAQPAVW
                     VDAATGKTWVFVSTGNGVSGLTLGLNSAVPPKPVLTKVWQNSTGGSSAVVANGVLYYF
                     SGSINALNPKTGAVLWSDASTGGVHWESPIVVNGRIYVTDESSQLWAYALNGVFKNGF
                     E"
     gene            complement(7374..8024)
                     /locus_tag="VFF05_06510"
     CDS             complement(7374..8024)
                     /locus_tag="VFF05_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZW51492.1"
                     /translation="MASKQQAYRGNEKQGWPAWAWLGIGVVLGIVLALLVLVRDWAPL
                     LHKSNLPQPNAQAVAPKASDQAVADAARPAAPPKKTFDFYSVLPEMEVVIPDAELSAK
                     AKAEQARQALAASQAQVQPAGTPTGTAPPALAESGNGYMLLAGSYNDAHAADEAKAKL
                     AMLGLVAKVQPVTINGKTWNRILLGPYADASATEAAKKTLADNGVKAIPISRKPGQ"
     gene            complement(8024..9724)
                     /gene="argS"
                     /locus_tag="VFF05_06515"
     CDS             complement(8024..9724)
                     /gene="argS"
                     /locus_tag="VFF05_06515"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZW51493.1"
                     /translation="MKDSLHQLVLHAIARLREQNVLPADAAAPAAPQHVSIERTKSRE
                     HGDFATNAALLLAKSARTSPRALAQALVAALPQNPQIAKVEIAGPGFINFFLAPAAYH
                     AQVRQVLESGAGYGRNATGANKTAGVEFVSANPTGPLHVGHGRNAAIGDCIARALAAN
                     GWNVKREFYYNDAGAQIANLAISVQARALGKGPDDAGWPEDGYRGEYIKDVAQAYLDG
                     ASVEIEGNLIVGAKDPHDLDAIRRFAVAWLRREQNADLAAFDVVFDTYFLESSLYAND
                     KVEETVRELVAHGHTYEEGGALWLRTTDYGDDKDRVMRKSDGSYTYFVPDVAYHRSKW
                     QRGYVRAITVLGSDHHGSLARVKAGLQALDCGIPQGYPDYMLYQMVTVMRGGEEVKLS
                     KRAGSYVTLRDLIDEVGRDATRYFLIARKADSQLVFDIDLARSQSNDNPVYYIQYAHA
                     RVASVMRQLAERGIAWNRENGVAKLACLDNEHETALMTELSRYPEIVESAAANLEPHV
                     IAQYLRELANALHTYYHAHQFIVDDADLRDARLALITAARQVLRNGLDLLGVSAPESM
                     "
     gene            complement(10082..>11123)
                     /locus_tag="VFF05_06520"
     CDS             complement(10082..>11123)
                     /locus_tag="VFF05_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51494.1"
                     /translation="PTQFPADDVERRVPDPATARALVRERNPQLLELLAYRRERFAPL
                     IAARTDHPPIVGAEDAVLIAFCRLAFRHGTWGDDFHAYHNEGHIFEILGPRLERLIDA
                     IGIASLSLRDWFLLALFAAAHDLRQRETPQFAAGIGSNERASIEETFRILHTCGYSEA
                     RNAEVFIGIELMIAGSTFDARPPMPALELNSAEVVVESGGALAQKLDAKLDKHAPGWR
                     EDARIVHALDLTLIAADLDTANVAEAFSLFMASAERLCLEREMRSHRNPASAASALPM
                     LNFLTSGQEHYFFDLHRFSSAPGRIAFAAAKEANIAPMRELSRDLRAAVPAPGTGNEV
                     LAAFHALVEKAH"
BASE COUNT         1987 a         3477 c         3621 g         2038 t
ORIGIN      
        1 gatctggcta cgcctgccga gcacgatctg ctcgatgtgg acgccgcgct cgatcgcctg
       61 gatgccatca atcctgattt gcgcgagttg gtcgaattgc gcatttttgc cggcatgacg
      121 ctggaggagt tggcgcaatc cagtggtcgc tcgttgcgca cggtcaatcg cgactggcag
      181 cgtgcgcgtg ccttgctgat gacgcagatg gcgcaagcgg tgtcgtgatg gacgcgcaag
      241 agcgcgacca atggcgtgcg gcggatcaac tgttccagcg cctgatcgaa ctcgatgacg
      301 ctgagcgccg ccgcgtgctg gagaccgttc aggtcgatgc aggcgttcac gccaagctcg
      361 cgcagttgct cgacagctcg cagcgctcgc accggatact cgacggcgat gcgctcaatc
      421 tgccttggca tgccgccggg ccagtgccac ccaatgcgga cggcgatcgt ctgctggggc
      481 gttgtatcgg caactggaag ctcgtcgaag cgatcggccg cggcggcatg ggggtggtgt
      541 atcgcgccga acgcgtgggc gcggattacg accagcgcgc cgcgatcaaa ctgctggcca
      601 tcgcgctgca cgccgatgcc gaagttcagc gctttcgacg tgagcgccgg atcctggcgc
      661 aactgcaaca cccgcacgtc gccggcctga tcgacgccgg cgtggccgag gacggcacac
      721 cttacctggc catggccctg atcgatggcg tgcgcatcga cggctggtgc gagcgacaag
      781 gcgccagcct gcgtgcgcgg gtcgaattga tgttgcaggt gtgcgatgcg gtcgcctacg
      841 cgcatcgcca actgatcgtg catcgcgaca tcaagcccgg caacgtcctt gtcgatgcca
      901 agggccaggc gacgttgctc gatttcggca tcgcccgcct gctcgatgcc gacgataccg
      961 gcgagcagac ggtcacccgt gcgttcacgc ccgactatgc cgcgcccgaa caacgccgcg
     1021 gcgagcgtgc gctcggtacc gccgtggacg tctatggtct cggcgcggtg ctgtatcgcc
     1081 tgctcgcggg cgcggcgccc gcatgcgatg ccaaaggcga ggtcgcaccg gcttcgcgcg
     1141 tggcgcgcga gcgcggcgat gcacgcggcg cggcactgtt gcgcggagac cttgacgcga
     1201 tcctcgcgca ggccttggct gaagtgccga cgcggcggta tgcgagcgtc gatgcgctgg
     1261 ccaacgacct gcaagcctgg ctgcaccatc gtcccttgcg cgcgcagcgt gccacggtgt
     1321 ggacgcgcct gcgcaagttc gtccgccgca accgtgccgc gagcgttctc gcgctcatcg
     1381 cgatacttgc tgccggcgcc ggatttgcga gtttcatttt gagccatcgc gcgctggaac
     1441 ggcgcgcggc gcaattgcag gccgtgacga aattccagac cgacatgttg atgcaattcg
     1501 cgcccgatcg ggccggcgcg agcctgcaca aggccgtggc cgatgcgctc ggcaagaccg
     1561 tgccggccgc aaacacggcc gcaaccgtgc tcaaccgcat cgattacacc ggcttgtcga
     1621 tcgacatgct ggacgaggcg attctcgcgc catcgatcga tgccgcgcac cagcgttttt
     1681 cggaccagcc gcgcgtgaaa ggcatgctgt tgctggcgct caccgaggct tatctgggcc
     1741 ttggccgatt cgacaaggcc agagccttgc aggagcaggt cacggctctg gcgcgcgagc
     1801 gcttcggcgc ggaggatcgc ctgacgctga tgagtttgcg cgacgagatg aaatgggtgc
     1861 gccaggcgaa atcgccggac ggcgaggcga tcagccggga catcgtgcac cagcatgagc
     1921 ggtacctcgg cgtgaatgac atcgacaccg ccatcgcgcg cggattgctc ggcgaatggc
     1981 tgatgcaaca tggcaagacg accgaggcgg aatccatcct gcgcgattcc ggaacacgca
     2041 tcgcccggga acgcgggcag gacgatccgg acgccgtctc ggcacgagcc aatctcgcct
     2101 tcgccatttc ctcgcaaggt cgctatgccg aatcggtacc cctgtatcgt tctgccgccg
     2161 aatccatggt gaaggtattc ggcgccgatc acccctacac cctgacgagc ctgaacaatc
     2221 tggcctatgc cttgaaccac agtggacacg gcgatgaagc cctgccgatt taccggcgcg
     2281 ttttcgaggc gcggcggcga gtgctcggcg cgaagcatcg ggcgaccttg atcagcgaaa
     2341 ataatctggc cgtgggtctg ggtcgtcagg gacagaatgc cgaagccgaa ccgctgcaac
     2401 gtgaggtgta cgccggcttt cgcgacacgc tcggggcgca cgatgcgacg acgctgcatg
     2461 ctggtctgaa tctggcgcgc aacgaactcg cgttgcggca ccacgccgat gtcgatgcgc
     2521 ttgtcgcaga actgctcgag ggttggcagc aacccaggga cgccaagccc atcgcgcagg
     2581 cgcagcgaat tctcgcccag agtctgcaag gccaaggcaa gtacgaggcg gcggcaacag
     2641 cgttcgagca atcctggcag gccgcaacaa tcgcaaacga cgccaaggaa cagcgtgaaa
     2701 ccgcgcaagc ggcactcgcg ccgggcgaca aggccaacgc gtcgcgacac agcctttggc
     2761 agcagcgcag ccaggcagtg gccagccaac cggaaaaacc atccgccgcg tccgatcaat
     2821 agcgtcgctg agggtgccat cggtcaaggc agcaaaccac ccgcttggga tcggcgctcg
     2881 cgccgatggc acggttttgc accgatttac gaataggcgc agaacaaccc acccaaggaa
     2941 agcgccatgc gagccctgcc aaaagccttt tttgccctga ttggcgtcgc gatgctgacc
     3001 gcgaccgcta acgcggccac ctaccgcatc ggttcgggct gtgcctatgc cgacctcggc
     3061 gcggcgatga cctacgccgc ggcgcacgat ttcgacagtc aggttgtttt ccaactgcgt
     3121 accggcacgg aattttccgg tgtgtacggc ccgaatgccg gctacgccag catcgaggtc
     3181 atcgatccgc gctgggacat ggtcatcgaa ggcggattcg acgattgcct cgccgccgtt
     3241 ccaaccggtt acacgacgct taagacttct cgcgccgccg acgggatgtt gctggtcgaa
     3301 aacggtctcc ttgcgcgcca cacggttgct attcgacgct tgcatttcgt cgacgccagc
     3361 gacacccagg ccgtgttctt ctgggacaac gtgacggcca tgctgggtac cggtatcgag
     3421 gtatccaaca acggcggcgg catcaacctg gcggcgcagg tcggcgcatc tagcgcgaac
     3481 tggccgcaat tgacgctcgg cgacggtgcg cgtgtccacg acaatctggg cagcggcatc
     3541 gctgggttgg gcacggttac cctggcgggt gcgagcatcg accacaacca ggccgagaac
     3601 ggtgcgggtg tccacctgtt cgagcctgga agtcgactca ttctcgacag caacggcggc
     3661 gacaattcga tcacggaaaa tgtcgccagc gcggcgggtg gcggcattta tctggatcat
     3721 gccagcctgc tgaccgtggc cgccgtctcc gacgtcaaca gcatctcgct caatcaggcc
     3781 ggaaccagca acaaccttgg tttcggctac ggaggcggca tctacagcga cggcggcgac
     3841 atcgaatttc attccggtgc cgcccatgcc ggacagcaca acatcctgga tggcaattcc
     3901 gccaactacg gcggcgcgat cgccgtggtc ggcgccaatg ccggggccgg cgggacttac
     3961 accttggtgt cgctgctcaa cacgcgtttt tcgtacaacc aagcctacgt tcgcggcggc
     4021 gcgctgtact cgcgcaatgc cgtggattgg accatcgacc gcggcaacct gagcggttgc
     4081 gatgcggatt tcttcgcgcc ctgctcggta ttcgccaata attccgcgcg caatgccgat
     4141 cgggcctacg atccggacac gccgcagccc aacggcggcg tgatttacct caccgacgaa
     4201 cgcagcgacg gctattcgcg cggcatcgcg cgcttctatc acacttattt cgccaacaat
     4261 tccgacgacc agggcagcgc ggccgtggcc gccgccactt catccgccga gctggtgttc
     4321 cagcgcgata tcttcgccgg caatgccgcg tatgtgctgg acgtgggcgc caatctgatc
     4381 gattcgcgca acacgaaaaa tctgtacttc ctctacaaca cggtattgcc caccaattac
     4441 gcgtattacg tgctgcacac gctgggcggt acggcggacc tcactggcag catcatcgaa
     4501 acaccggaac tgctcggcgt cccggtaccc atctggtacg gcgaaggatc ggcgcaggtc
     4561 gtgcaccgag gctgcctggt ctcgctaagc gccctgatcg acgccaacgg cagagccggc
     4621 tgggtcggtg atgcgcgcat cagcgccacg gatttcgcgc cggctggcgg ctcgccggcc
     4681 atagaccatt gcgccttcac cccgcccatc gacttctacg gcttcgccgg cgccgtcgac
     4741 accaagggcg tgccggcgcg cttcggcaac tacgacctcg gcgcggtcga gcagcgcgac
     4801 atcatcttct acaacgattt cggcattcgg ccgaacggct gacggtccgc cgcaggttga
     4861 gtttccgcgg tggcgtcgtg caaaccgagc gtcggattgg gttcagttgt cccgcgcgat
     4921 ctgaaacccc gcgaacgact ggctgaccgg catcacttcg atgcggttga tgttcagatg
     4981 cgccggcagg ttggcgaccc accagatcgt ttcggcgatg tcgtggccgg tgatcgggtg
     5041 cgcgccctgg tagagcttgt cggacgcgac ttggtcgccg ccattgcgca cgagggtgaa
     5101 ttcggtttcg gccatgcccg gttccacgct cgtcacgcgc acaccgctgc cgtgcaggtc
     5161 gcagcgcagg cccagcgaaa actgggtgac gaaggccttg gtgccgccgt aaacgttgcc
     5221 ggcggtgtag ggataactcc ccgcgaccga gcttacattg acgatgctgc cgcggcgctc
     5281 gatcagcgtc ggcagcagct tgtgcgtgag cgtcgccagc gcagtgatgt tggtgtcgat
     5341 catctgccgc cactgcgcga gatcgcagtg ttgcgccttg gccgtgccga gtgcgagtcc
     5401 cgcgttgttg accagcatgt cgatgccacg gaattccgcg ggcagggcgt cgagtgccgt
     5461 ctgcatcgcc gccgcgtcgc ggacatcgaa cgccgccgtg tgcacgctcg cgccgaattc
     5521 gtcggtgaga tgttgcaggc gatcgccgcg cctcgcgcag gcgaccacgc gccagccagc
     5581 ttgtgcgaaa cgtcgcgcgg tggcggcgcc gaatccggcg ctggcgccag tgacgaggat
     5641 ggttttggca gtcatggcca ttttccgacg caaaggataa cgataacgga aaacgcaggg
     5701 tatgcgcgac gttatgttgt cgcgaatttg tcgcgtgtgc gcattttcac gaaaacgcag
     5761 gggcagcggt gctaggttgc ttgcgcagaa atttttgcag tttatcgagc gaggcatgtc
     5821 atgcgcggcg atctggcatt ccggtcagtc atcctggttg caggcacgtt gcttgttgcg
     5881 atatccgcgc ccgctgcgga ctggatgcag ttcggcttcg atccggcaca cagcggcaac
     5941 aatccggccg agacaacgct ttccgccagc gctacgggcg aattcgcgct gacggcatta
     6001 cttccgatct acaccgtcac cctgcccgat atcgccgacg gcgcacctgc atttctttcc
     6061 gccgccgcga cgacaaatgg cagtcgcgat ctgctgtttc tggagaccaa agacggtcac
     6121 gcgcttgcga tcgatgccgc gacaggcgcg ctcgtgtggt cgcatgcgac caatccctcg
     6181 ccgccgccgg ctggacagga ctacaccact tcgtcgccgg caatcgatcc gaatcgtcaa
     6241 tacgtatatt tctacgggct cgacggctat gtgcacaaat atgccgtcgg caccggtttg
     6301 gagacgacga gcggcggctg gccagagctg acgacgaaca agcccgacgt tgaaaagggc
     6361 tcgtccgcgc tcgccatcgc caccgcgaaa agcggcacga cctacctgta cgtcaccaat
     6421 ggcggttatc cgggcgacgc cggcgattat cagggtcatg tcaccgtaat caacctcgcg
     6481 accggtgcgc aaaacgtgtt caacgccaac tgcagtgatc agaccgtgca tttcgtggag
     6541 aacggcacac ccgattgcgc gcaggtacag acggcaatct gggcgcgacc gggggtgatc
     6601 tactcctcgg ccaccgatca tgtctacatg tctaccggca acggcacgtt cgatgccaat
     6661 gcgggtgggc acgactgggg cgacagcgtg ttcgcgttga atccggatgg cacgggcagc
     6721 ggcggcaatc cgatcgacag ctacacgccg acgaacttct tgacgctgga ttctagcgat
     6781 gcggatctgg gatccacggc accggcgatc ttgccgtcga cgctggcggg ctacccggat
     6841 ctggccgtac aaggaggcaa ggaccagaaa ctgcgtttgc tcaatctgga taatctcagc
     6901 agcagcgcca cggtgggacc ggggcatacc ggcggtgagt tgcaattgat caatgtcccg
     6961 cagggcggac tggtgttcgc gcaaccggcg gtatgggtcg atgctgcaac cggcaagacg
     7021 tgggtgttcg tctccaccgg caacggcgtg agtggactga ctctcggttt gaatagtgca
     7081 gtacctccaa aaccggtgct caccaaggtg tggcagaaca gcactggcgg ttcgtctgct
     7141 gtggtcgcca atggcgtgct ctactacttc agcggttcca tcaatgcgct caatccgaaa
     7201 accggcgcgg tactgtggtc ggacgcgtcg accggtggcg tgcactggga aagccccatc
     7261 gtcgtcaacg gccgcatcta cgtcacggat gaaagtagtc agctctgggc atacgcgctg
     7321 aacggcgttt tcaagaacgg attcgagtag cgcgcaaatt aggccggcct ggattactga
     7381 cccggcttgc gcgaaatcgg aatcgccttc acgccgttgt cggcgagcgt cttcttcgcc
     7441 gcttcggtcg cgctggcgtc cgcgtaaggg ccaagcagga tgcgattcca ggtcttgccg
     7501 ttgatcgtca ctggctgcac cttggcgacc aggccgagca tcgcgagctt ggcttttgct
     7561 tcgtccgccg cgtgcgcgtc gttgtacgaa ccggccagca gcatgtagcc attgccggat
     7621 tcggcgagtg cgggtggcgc cgtgcctgtc ggtgtgccgg ccggttgtac ctgtgcctgc
     7681 gaggcggcca gcgcctggcg agcctgctcg gccttggcct tggcggaaag ttcggcgtcg
     7741 ggaatcacca cttccatttc cggcagcacc gaatagaaat cgaaggtctt cttcggcggc
     7801 gcggcgggcc tggccgcatc ggcgaccgcc tgatcgctgg ccttgggtgc gaccgcttgt
     7861 gcgttgggct gtggaagatt tgatttatgc agcagcggcg cccaatcgcg gaccagaaca
     7921 agcaaggcca ggacgatacc cagcaccacg ccaatgccga gccaggccca ggccggccag
     7981 ccctgttttt cgttgccgcg ataagcttgt tgcttgcttg ccattacatg gactccggtg
     8041 ccgaaacgcc gagcaggtcg aggccgttgc gcagcacctg gcgcgcagcc gtgatcaggg
     8101 ccaggcgcgc gtcgcgcaga tcggcgtcgt cgacgatgaa ctgatgggcg tgatagtagg
     8161 tatgcagcgc gttcgcgagc tcgcgcaggt attgcgcgat cacatgcggc tccaggttcg
     8221 ccgccgcgga ttcgacgatc tcgggatagc gcgacagctc cgtcatcagc gccgtttcgt
     8281 gttcgttgtc caggcacgcg agtttggcca cgccgttttc gcgattccac gcgatgccgc
     8341 gctcggcgag ttggcgcatc acgctggcca cgcgcgcgtg ggcgtactgg atgtagtaga
     8401 ccggattgtc gttggactgc gaacgcgcca gatcgatgtc gaagacgagc tgcgaatcgg
     8461 ccttgcgcgc gatcaggaaa taacgcgtcg cgtcgcggcc gacttcgtcg atcaggtcgc
     8521 gcagggtgac gtaagagccg gcgcgcttgg acagcttcac ttcttcgccg ccgcgcatca
     8581 ccgtgaccat ctggtacagc atgtagtcgg gatagccctg cgggatgccg cagtcgagcg
     8641 cctgcaggcc ggctttcacg cgggccagcg aaccgtgatg gtcggagccc agcacggtga
     8701 tcgcgcgcac gtagccacgc tgccatttgc tgcggtgata agccacgtca ggcacgaaat
     8761 aggtgtagct gccgtcggac ttgcgcatga cgcggtcttt gtcgtcgccg tagtccgtcg
     8821 tgcgcagcca cagcgcgccg ccttcctcgt aggtgtggcc gtgggcgacg agttcgcgca
     8881 cggtttcttc caccttgtcg ttggcgtaca gcgaggattc gagaaaatac gtatcgaaaa
     8941 cgacgtcgaa tgcggccagg tccgcgttct gctcgcggcg cagccaggcg acggcgaaac
     9001 ggcggatcgc gtccaggtcg tgcggatcct tcgcgccgac gatgagattg ccctcgattt
     9061 cgacgctggc gccgtcgaga tacgcctgtg cgacatcctt gatgtattcg ccgcggtagc
     9121 cgtcttcggg ccaaccggcg tcgtccggac ctttgcccag cgcacgcgcc tgtaccgaga
     9181 tcgccagatt ggcgatctgc gcaccggcat cgttgtaata gaactcgcgc ttgacgttcc
     9241 agccgttcgc ggcgagcgcg cgcgcgatgc aatcgccgat cgcggcgttg cggccgtgac
     9301 cgacgtgcag cgggccggtc gggttggcgg agacgaactc cacgccggct gtcttgttcg
     9361 cgccggttgc gttgcggcca tagccagcgc cggattccag cacctgacgc acttgcgcgt
     9421 ggtacgcagc cggagcgagg aagaagttga tgaagccggg gccggcaatt tccacttttg
     9481 cgatttgtgg attttgcggc aatgccgcga ccagcgcctg cgccagcgcg cgcggactcg
     9541 tgcgcgcgga cttggccagc aacagcgcgg cgttcgtggc gaaatcgcca tgttcgcggc
     9601 ttttcgtgcg ttcgatcgag acgtgctgcg gcgcggcagg cgcagcggcg tcggcgggca
     9661 aaacgttttg ttcccgcagg cgcgcgatgg cgtgcaggac gagttggtgc agggagtctt
     9721 tcacgcagag gggttccgga aatcgcacgc tattgtagac ggcgcggcgc gtacgccaaa
     9781 ggcacccgcg cacgcgtcgg ccagctcaac gccaaaaccg catgacagtg cggtttccaa
     9841 gcctgtggat aactctgtgc gtatcgcccg acgcggaaga ctgcggagcc aaagacaatt
     9901 cacggcgatt gttggccgga gcgggttgaa tacgtctata tcattgatat ttcggaagaa
     9961 atatcgtaat ggattcgtga caaattttgg caatttggca caatgcgacg aatgcagcat
    10021 tgtgtataaa tccaccacgg agaagcgcat tccgccgccg aattcacgct ggatcgcttc
    10081 gctaatgcgc tttttcgacg agtgcgtgga acgctgccaa tacctcgttt ccggtgcccg
    10141 gcgccggcac ggcagcgcgc aggtcgcggg acaactcgcg catgggcgcg atgttcgcct
    10201 ctttggccgc ggcgaaagcg atacggcccg gcgcggagct gaagcggtgc aaatcgaaaa
    10261 aataatgttc ctgcccggaa gtgaggaaat tcagcatggg cagcgcggaa gcggcgctgg
    10321 ccggattgcg gtggctgcgc atttcgcgct ccaggcacag gcgttcggcg cttgccatga
    10381 acagcgaaaa cgcctcggcc acgttggcgg tatccaggtc cgcggcgatc agggtcaggt
    10441 ccagcgcgtg cacgatccgc gcgtcctcgc gccagcccgg cgcatgtttg tcgagcttgg
    10501 cgtcgagctt ttgcgccagc gcgccgccgg attcgaccac gacttcggcg ctgttcaatt
    10561 ccagagcggg catcggcgga cgcgcgtcga acgtgctgcc ggcgatcatc agttcgatgc
    10621 cgatgaatac ttcggcattg cgcgcttcgc tgtatccgca agtgtgcagg atacgaaacg
    10681 tttcctcgat gctggcgcgc tcgttgctgc cgatgccggc ggcgaattgc ggcgtctcgc
    10741 gctggcgcag atcgtgggcg gcggcgaaca acgcgagcag gaaccagtcg cgcaggctca
    10801 gcgaggcgat gccgatcgca tcgatcaggc gttccaggcg cggaccgaga atctcgaaga
    10861 tgtggccttc gttgtggtaa gcatggaaat cgtcgcccca ggtgccgtgc cggaacgcca
    10921 gacggcaaaa cgcaatcagc acggcgtcct cggcgccgac gatcggcgga tggtcggtgc
    10981 gcgcggcgat cagcggggcg aagcgttcgc gccggtaggc gagcagttcg agcaattgcg
    11041 gattgcgttc gcgcacaagc gcacgcgccg tggccgggtc cggcacgcgg cgttcgacat
    11101 cgtccgcggg aaattgcgtg gga
//
LOCUS       DASRVO010000525         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_301545, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000525 DASRVO010000000
VERSION     DASRVO010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301545"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..2138
                     /gene="mnmG"
                     /locus_tag="VFF05_06525"
     CDS             261..2138
                     /gene="mnmG"
                     /locus_tag="VFF05_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZW51495.1"
                     /translation="MSQQIPNYDVIVIGGGHAGTEAALASARLGARTLLLTHNIETLG
                     QMSCNPAIGGIGKGHLVKEIDALGGAMARAADRAGIQWRTLNASKGPAVRATRCQADR
                     ALYKATIRRKLETQLNLSIFQQSVEDLTLVGNRVTGAVTQMGLVFSAHSVVLTAGTFL
                     AGKIHVGLENFAGGRAGDPPAIGLAARLRELPLRVDRLKTGTPPRIDGRSIDFTCLAE
                     QPGDDPMPVFSFMGTTEEHPRQVSCWITHTSERTHEIIRGALDRSPLYTGKIEGIGPR
                     YCPSIEDKVVRFAEKASHQIFVEPEGLETTEIYPNGISTSLPFDAQVELVRSIRGFEN
                     AHITRPGYAIEYDYFDPRGLKASLETKAIEGLYFAGQINGTTGYEEAAAQGIVAGINA
                     ARAVQDKAPWTPRRDEAYIGVLIDDLITNGTSEPYRMFTSRAEYRLHLREDNADLRLT
                     ETGFEMGSVDAPRHDAFCRKRDAIARETERLRGIWAAPGNALGREIEATLRVPLSRET
                     NALDLLRRPELNYAQLMHVPTLGPAVEDAQVAEQVQVAAKYSGYLDRQNEEIARQRRH
                     EETQIPAAFDYAAVRGLSAEVMQKLVRGRPQTIGQAQRISGVTPAAISLLLVHLRRRA
                     A"
     gene            complement(2162..3007)
                     /locus_tag="VFF05_06530"
     CDS             complement(2162..3007)
                     /locus_tag="VFF05_06530"
                     /inference="COORDINATES: protein motif:HMM:NF021158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HZW51496.1"
                     /translation="MRTPVFPAVLALGLLAGCNSQPQPPAPAAPQIANNVSGTIMLRE
                     PRALGDNARAELKVVDVSQPDTPLAQVTIEHANHPPINLVLPIDPKAVDPLRTYAVDA
                     KLIDGERRFLPVLQYPVLTKKSPSVVQIVLAPEPTPAEKMYEAYKKAFAEIGTFKSVS
                     GGGQTPNTSTAWDAFYSNGKVKVIREITDLYDDKANETGRVTMKMAYQNDKPWVVVKE
                     ESSGDGARPSAVTKVGWDDSGALVLKDKMAGGASQATPEEANALYQHAVQALAQAQQK
                     VPKPK"
BASE COUNT          610 a         1045 c         1061 g          609 t
ORIGIN      
        1 aatccggcgc gacgacctga cgcaacatga ccttgttcac acttccctaa gcagcatttg
       61 agttaagatg cgccgcaaat ccctgcgagc atgcgctatc cgctccgcac ggttcggtca
      121 ctggcgcccg gcatacggtc ctcttggatt cccctgttcc taagaccgcc gggcgccagc
      181 cttatcgcaa gacctcccaa gcggcgcttc ggcgccgctt ggcgtttgta taatccgcgc
      241 gccgcaaaga cgcgacagcc atgagccagc aaatacctaa ttacgacgtc atcgtcatcg
      301 gcggcggcca cgccggcacc gaggcggcgc tcgcgtccgc tcggctgggc gcgcgcaccc
      361 tgctgctgac ccacaatatc gaaaccctgg gtcagatgag ctgcaatccg gccatcggcg
      421 gcatcggcaa gggccatctg gtcaaggaga tcgacgcact cggcggtgcg atggcgcggg
      481 cagccgaccg tgccggtatc caatggcgca cgctgaatgc ctccaagggc ccggccgtgc
      541 gcgccacgcg ctgccaagcc gaccgcgcac tgtacaaggc gacgatccgg cgcaagctgg
      601 aaacccagct gaatctttcc atcttccagc agtcggtcga ggacctgacg ctcgtcggca
      661 atcgcgtcac cggcgcggtg acgcagatgg gcctcgtgtt ttccgcgcac agcgtcgtgt
      721 tgaccgcggg aaccttcctc gccggcaaga tccatgtcgg cctggaaaac tttgcgggag
      781 gccgcgcggg cgatccaccg gccattggcc tggccgcgcg cctgcgcgag ctgccgttgc
      841 gcgtggatcg gctgaaaacc ggtacgccgc cacgcatcga cggacgcagc atcgatttca
      901 cctgtctggc cgagcagccg ggcgacgatc ccatgccggt gttttcgttc atgggcacga
      961 cggaggaaca tccgcgtcag gtcagttgct ggatcacgca cacgagcgag cgcacccacg
     1021 agatcatccg cggcgcgctg gatcgttcgc cgttgtatac cggcaagatc gagggcatcg
     1081 gtccgcgcta ctgcccttcg atcgaggaca aggtcgtgcg ttttgccgag aaggcgtcgc
     1141 accagatctt cgtcgagccg gagggcctgg aaaccactga aatctatccg aatggcattt
     1201 ccacctcgct gccattcgac gctcaagtgg aactggtgcg cagcatccgc ggtttcgaga
     1261 acgcgcacat cacccggccc ggctatgcca tcgaatacga ttatttcgat ccgcgcggct
     1321 tgaaggcctc gctggaaacc aaggcgatcg agggtttgta tttcgctggc cagatcaatg
     1381 gcacgaccgg ctacgaggaa gcggccgcgc agggcatcgt cgccggcatc aacgccgcgc
     1441 gcgcggtgca ggacaaggcg ccgtggacgc cgcgccgcga cgaggcttac atcggcgtat
     1501 tgatcgacga cctgatcacg aacggcacca gcgaaccgta tcgcatgttc acctcgcgcg
     1561 cggaataccg cctgcatctg cgcgaggaca acgccgatct gcgcctgacc gaaacgggct
     1621 tcgagatggg cagcgtggat gccccccgcc atgacgcgtt ctgccgcaag cgcgacgcga
     1681 tcgcgcgcga aaccgaacgc ctgcgcggca tctgggccgc gccgggcaat gcgctgggac
     1741 gcgagatcga agcgacgttg cgcgtgccgc tgtcgcgcga aacgaacgcg ctggacttgt
     1801 tgcgccggcc ggaacttaat tacgcgcagc tcatgcacgt gccgacgctg ggccccgccg
     1861 tcgaggacgc gcaagtagcc gagcaggtgc aggtcgcggc caaatattcc ggttacctgg
     1921 atcgccagaa cgaagaaatc gcgcgccagc gccggcatga ggaaacgcaa atcccagcgg
     1981 cattcgatta cgcggcggtg cgcggcctgt ccgcggaggt gatgcagaaa ctcgtgcgcg
     2041 gtcgtccgca gacgatcggt caggcacaac gcatatccgg cgtcacgccc gcagccatct
     2101 cgctattgct ggtgcatctg cgccgccgcg cggcgtgaag cggccgcgcg actgaaatcg
     2161 cttatttggg cttgggcact ttttgctgcg cttgggccag agcctgcacg gcgtgctgat
     2221 atagcgcatt ggcttcttca ggcgtggcct ggctggcgcc gccggccatc ttgtctttca
     2281 ggaccaatgc gccgctgtcg tcccagccga ctttcgtgac cgccgaagga cgcgcgccat
     2341 cgccgctgga ttcttccttg accacgaccc agggcttgtc gttctgataa gccattttca
     2401 tcgttacacg accggtttcg ttggccttgt cgtcgtaaag gtcggtgatt tcgcggatga
     2461 ccttgacctt gccgttgctg tagaacgcat cccaggcggt ggaggtattc ggcgtctgtc
     2521 cgccgccgct caccgacttg aacgtgccga tttcagcgaa ggccttcttg taggcctcgt
     2581 acattttctc cgccggagtc ggttccgggg caaggacgat ctgcaccacc gatggcgatt
     2641 tcttggtcaa caccggatat tgcagcaccg gcaggaaacg ccgttcgccg tcgatcagct
     2701 tggcgtcaac ggcgtacgtg cgcagggggt cgacagcctt gggatcgatc ggcaggacga
     2761 ggttgatcgg cggatggttg gcatgttcga tggtgacctg ggcgagcgga gtgtccggtt
     2821 gcgagacatc caccaccttc aattcggcgc gagcattgtc gccgagagca cgcggttcgc
     2881 gcagcatgat ggtgccgctg acgttgttcg cgatctgcgg tgcggccggt gcgggcggtt
     2941 gcggctgcga attgcagccc gcgagcaggc cgagcgccaa tacggcggga aaaacagggg
     3001 tacgcatggg cattctccgg aaagcgacgt gcggaaaacc cgcaaaagcc gcagtgggtc
     3061 atgagattaa ggagaagccg ccgctgccga ggcaggccag cacttccgtc ttgtccaact
     3121 attctagtgc gcgccgggcc ggattgaaaa ccaaaccata gtcatggtcc ggattggtct
     3181 gttgcggcgt gaggctgccc gatgccaggt cgtaaattcg caaacgtaaa aaaattgaca
     3241 ggccagttaa ctatttgtta cgttcggcag gctggctggc gccgacttct gggggcgagc
     3301 ttgcgtgcgt gggggaatct tgccg
//
LOCUS       DASRVO010000526         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_15597, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000526 DASRVO010000000
VERSION     DASRVO010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15597"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /gene="sbcB"
                     /locus_tag="VFF05_06535"
     CDS             <1..353
                     /gene="sbcB"
                     /locus_tag="VFF05_06535"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF020007.4"
                     /note="Exonucleolytic cleavage in the 3'- to 5'-direction
                     to yield nucleoside 5'-phosphates; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HZW51497.1"
                     /translation="DKRLFAKIRGTLPAALRDHENAFHDPRYSTLLFRYRARNFPETL
                     NADENRRWQSFRRQRLTRSTSITSPTLDDYFAQTAALRANPATNGAQQALLDQLEQWG
                     HHVARDIDYTLASP"
     gene            350..1036
                     /locus_tag="VFF05_06540"
     CDS             350..1036
                     /locus_tag="VFF05_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HZW51498.1"
                     /translation="MSKTYFSKNTFAFLRTLDRNNNRPWFHAHQGDYERHVRGPFLQL
                     IADLQTPLAKISMHMRADPRKAGGSLFRIHRDTRFANDKTPYKPWAGARLFHERRREV
                     AAPSFYLHIAPRDCFAGGGIWHPEPPTLKRIRDFLADNPAAWKRATQGKAFREHFAFW
                     GESLSRPPRGYPVDHELIEDLKRKSYAAGEEFSEALACSSELQPFAVATFKRLAPMID
                     YLCAALELEF"
     gene            complement(1040..>1526)
                     /locus_tag="VFF05_06545"
     CDS             complement(1040..>1526)
                     /locus_tag="VFF05_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZW51499.1"
                     /translation="FTAGDTELHAALGNVRVDATDLSRDDWLDLLLTHRIQPNFPGDR
                     ITVVFDYPASQCALAKIRNGDPPVAERFELYAGTQELANGYHELTDAVEQRMRFVRDK
                     ARRQERGLPELPLDENFLAALESGLPDCAGVALGVERLLMAMMATDDIRDVLAFDFAR
                     A"
BASE COUNT          293 a          525 c          444 g          264 t
ORIGIN      
        1 ccgacaagcg cctgttcgcc aagattcggg gcacgctgcc ggcagcgttg cgcgaccatg
       61 aaaacgcgtt ccatgacccg cgctattcga ccctgctgtt ccgctatcgt gcgcggaatt
      121 ttccggagac gctgaacgcc gacgaaaacc ggcgctggca atcgttccgc cgccaacgcc
      181 tcacgcgatc gacctcgatc acatcgccga ccctggacga ttacttcgcg cagaccgcgg
      241 cactgcgtgc gaatccggca acgaacggcg cgcaacaggc cttgctcgac cagctcgaac
      301 aatggggcca tcatgtcgcg cgggacatcg actacaccct cgcatcgcca tgagcaaaac
      361 ctatttttcc aaaaacacct tcgccttcct gcgcacgctg gaccgcaaca acaaccggcc
      421 gtggttccac gcgcatcagg gcgattacga acgccatgtg cgcgggccct tcctgcaact
      481 gatcgccgac ctgcagacgc cattggcgaa aatcagcatg cacatgcgcg ccgatccgcg
      541 caaggccggc ggctcgctgt tccgcatcca tcgcgacacg cgcttcgcca acgacaagac
      601 gccgtacaaa ccctgggccg gcgcgcgcct gttccacgag cgccgccgcg aagtcgccgc
      661 gccatcgttc tacctgcata tcgcacccag ggattgtttc gctggcggcg gcatctggca
      721 tccggaaccg ccgacgctca agcgcatccg cgatttcctc gccgacaatc ccgccgcgtg
      781 gaagcgcgcc acgcagggca aggcattccg tgagcatttc gcgttctggg gcgagagcct
      841 gtcgcgcccg ccgcgcggct atcccgtcga tcacgaactg atcgaggact tgaagcgcaa
      901 gagttacgcc gccggtgaag agttcagcga agcgctcgcc tgctcatccg aattgcagcc
      961 ctttgccgtg gccacgttca agcgcctggc gccgatgatc gattacctgt gtgcggcgct
     1021 cgagttggaa ttctgatcgt caggcgcgag caaaatcgaa tgcgagcaca tcgcggatat
     1081 cgtccgtagc catcatcgcc atcagcaggc gttcgacgcc gagcgcgacg ccggcgcagt
     1141 ccggcaatcc gctttcgagt gcggcgagaa aattttcgtc cagcggcaac tccggcaagc
     1201 cgcgttcttg ccggcgcgcc ttgtcgcgca cgaagcgcat gcgctgttcg accgcatcgg
     1261 tcaactcgtg gtagccgttc gccagttcct gcgtaccggc gtacaactcg aagcgttcgg
     1321 cgaccggcgg atcgccattg cggattttcg cgagcgcgca ttgcgacgcc ggatagtcga
     1381 acacgaccgt gatgcgatcg ccgggaaaat tcggctggat gcggtgcgtg agcaacaagt
     1441 ccagccagtc gtcacgcgac aggtccgtcg catcgacacg cacgttgccg agcgcagcgt
     1501 gcaattccgt atcgcctgcc gtgaac
//
LOCUS       DASRVO010000527         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_144666, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000527 DASRVO010000000
VERSION     DASRVO010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144666"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1658
                     /locus_tag="VFF05_06550"
     CDS             57..1658
                     /locus_tag="VFF05_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HZW51500.1"
                     /translation="MTALTDEIARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGSV
                     KSRKASRHATSDWMALEKERGISVTSSVMQFPYEQCIVNLLDTPGHADFSEDTYRVLT
                     AVDSALMVIDCAKGVEERTIKLMDVCRLRDTPIMAFINKLDREGRPPIELLDEVESVL
                     KIQCAPVTWPIGMGSRLKGVYHLLTGEVHVFEPGRNFTRQDSTIFASLDDPALEAKIG
                     ADMLRELREELELVEGASHPFDAQAYLDGRQTPVFFGSAVNNFGVQLLLDFFVEHAPA
                     PRPRATTTREVNPEEGKLTGFVFKIQANMDPMHRDRVAFMRICSGEFDGGMKAFHVRT
                     AKEVKLANALTFMASERDIVEKAYPGDVIGIHNHGTISIGDTFTEGEALSFTGIPNFA
                     PELFRRARLKDPLKMKQLTKGLAQLSEEGATQFFRPLMSNDLILGAVGVLQFDVVAYR
                     LKDEYGVDCAFENVTVSTARWIRCNDEKMLAEFRDKAAMNLALDAAGELVYLAPSRVN
                     LQLTQERWPGVTFAATREHASSVAA"
     gene            1786..>2268
                     /locus_tag="VFF05_06555"
     CDS             1786..>2268
                     /locus_tag="VFF05_06555"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HZW51501.1"
                     /translation="MHARKIPAIAGLLVLFALCGCAPAGTPIRPVSPVDLTRFMGRWY
                     VIASIPTRFERGGHNPVETYSRESDGTICTWFRLRPGNFEAPVKLIHSSASPVAESGN
                     GEWRVRLFGILSMQYLVGWLNADYSQVLVVRDARDYLWFMARTPEVSDADYRAMLDRA
                     K"
BASE COUNT          419 a          724 c          727 g          398 t
ORIGIN      
        1 atagggaagc ggcgctgcgc tgcgcatcgc cgctcccgca gccgcccgcc ctgtcgatga
       61 ctgccctgac ggacgaaatc gcgcgccgac gcacctttgc gatcatctcg catcccgatg
      121 ccggcaagac cacgctgacc gagaagttgc tgctgttcgg cggcgcgatc cagatggctg
      181 gcagcgtcaa gtcgcgcaag gcgtcgcgcc acgccaccag cgactggatg gcgctggaaa
      241 aggagcgcgg catctcggtg accagctcgg tgatgcagtt cccgtacgag caatgcatcg
      301 tcaacctgct cgacacgccg ggccacgccg acttttccga ggacacctac cgggtgctga
      361 ccgcggtcga ctcggcgctg atggtgatcg actgcgccaa gggcgtcgag gagcgcacga
      421 tcaagctgat ggacgtgtgc cggctgcgcg acacgccgat catggccttc atcaacaagc
      481 tcgaccgcga aggccggccg ccgatcgagc tgctcgacga ggtcgagtcg gtgctgaaga
      541 tccagtgcgc gccggtgacc tggccgatcg gcatgggctc gcgcctgaag ggcgtctatc
      601 acctgctcac gggcgaggtg cacgtgttcg agccggggcg caatttcacg cgccaggatt
      661 cgacgatctt cgcctcactc gacgatccgg cgctcgaagc gaagatcggc gcggacatgc
      721 tgcgcgaatt gcgcgaagaa ctggaattgg tcgagggcgc gtcgcatccg ttcgatgcgc
      781 aggcctatct cgatggccgg cagacgccgg tgttcttcgg ttctgccgtc aacaatttcg
      841 gcgtacagct gctgctggat ttcttcgtcg aacacgcgcc cgcacccagg ccgcgcgcta
      901 ccacgacgcg cgaggtgaat ccggaagaag gaaaactcac cggcttcgtg ttcaagatcc
      961 aggcgaacat ggatccgatg caccgcgacc gcgtggcgtt catgcgcatc tgctcgggcg
     1021 aattcgacgg cggcatgaag gcattccacg tgcgcacggc caaggaggtc aagctcgcca
     1081 atgcgctgac gttcatggcc agcgagcgcg acatcgtcga gaaggcgtat ccgggcgacg
     1141 tgatcggcat ccacaaccac ggcacgatct cgatcggcga tacgttcacg gaaggcgagg
     1201 cgctttcctt caccggcatc ccgaacttcg cgccggaact ctttcggcgc gcgcgcctga
     1261 aggatccgct gaagatgaag cagctgacga agggattggc gcagctgtcc gaggaaggtg
     1321 cgacgcaatt cttccggccc ctgatgagca atgatctgat cctcggcgcg gtcggcgtgc
     1381 tgcagttcga cgtggtcgcc taccggctca aggacgaata cggcgtcgac tgcgcgttcg
     1441 agaatgtcac cgtgtcgacg gcgcgctgga tccgctgcaa tgacgaaaag atgctcgccg
     1501 aattccgcga caaggcggcc atgaaccttg ccctggacgc cgcgggcgaa ctcgtctacc
     1561 tcgcgccctc gcgtgtgaac ctgcaattga cgcaggagcg gtggccgggc gtgacgtttg
     1621 ccgccacacg cgagcatgcg agttcggtcg cggcctgaaa aggaaatccc ggccggatgc
     1681 gcggccggtg gatgctgtcg aaaaaaccac acgccggcct tcacgctgcc ttgcgcggct
     1741 gcctcgtagc attagaccct tccattgccg aaggcgcatc caatcatgca tgcacgcaaa
     1801 attcctgcga tcgccggact gctcgtcctg ttcgcgctgt gcggttgcgc gccggcgggt
     1861 acgcccatac gcccggtttc cccggtcgac ctgacgcgat tcatgggccg ctggtacgtg
     1921 attgcctcga tcccgacccg attcgaacgg ggcggccaca acccggttga aacctacagc
     1981 cgcgaatccg atggaaccat ctgcacgtgg ttccgtctgc gcccggggaa tttcgaggct
     2041 ccggtcaaac tcatccactc cagcgccagt cccgttgcgg aaagcggaaa cggcgaatgg
     2101 cgggttcggc tgttcggaat cctttcgatg caatatctcg tcggatggct gaacgccgac
     2161 tacagtcagg tgctggtcgt gcgcgatgcg cgcgactacc tgtggttcat ggcgcgcacg
     2221 ccggaagtct ccgatgcgga ctatcgggcg atgctggacc gggccaag
//
LOCUS       DASRVO010000528         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_275681, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000528 DASRVO010000000
VERSION     DASRVO010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275681"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFF05_06560"
     CDS             <1..294
                     /locus_tag="VFF05_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW51502.1"
                     /translation="FAAALAAIAIAASSFTRQTVVAAIAALAIGLVLWMLDAGARYEG
                     VSSSFINYLALPTHLEPFLRGIVATVDVVYFALIVTVALTLAARRLDAERERG"
     gene            294..>1148
                     /locus_tag="VFF05_06565"
     CDS             294..>1148
                     /locus_tag="VFF05_06565"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HZW51503.1"
                     /translation="MLARIKGFVFTALLVVAALCVAFLSTRFGWQHDFSYAQRSSLDA
                     RSVDLLHRLDAPVAIVSYAGQDAQLRAAIADFVARYQRIKPDISLRFVDPDADPAATR
                     AAGISIDGELAVTYKQHAEQLKVLTEREFDNALVRLARTRVRLVAFLEGDGERKPDGK
                     ANADLGNFGALLAEQGVRTLDLTLSDGMHVPQNVDVLVLASPQVALAPALVDELADYL
                     ARGGNFLWLTEPGANAGLDALAKALSLRVLDGTVVDGAGVALGLGDPSFVAISTYPQN
                     SITRDFALT"
BASE COUNT          166 a          371 c          391 g          220 t
ORIGIN      
        1 ttcgccgccg cgctggccgc catcgcgatt gccgcgtcga gcttcacgcg acagacggtt
       61 gtcgcggcca tcgccgcact ggcgatcggc ctggtgctgt ggatgctcga tgccggcgca
      121 cgctacgagg gcgtgagcag cagtttcatc aattacctcg cgctgcccac gcatcttgaa
      181 ccgtttctgc gcggcatcgt ggcgacggtg gatgtcgtct acttcgcgct gatcgtcaca
      241 gtggcgttga cgctggccgc acgccgtctc gacgcggaac gggagcgcgg ctgatgctcg
      301 cgcgcatcaa gggtttcgtc ttcaccgcgc tgcttgtcgt ggcggcactg tgcgtcgctt
      361 tcctgtccac gcgcttcggc tggcagcacg atttcagtta tgcgcagcgc tcgagtctcg
      421 acgcgcgcag cgtggatttg ctgcatcgtc tcgatgcgcc cgtcgccatc gtttcctatg
      481 ccgggcagga tgcgcagttg cgcgccgcca tcgccgattt cgtcgcacgt taccagcgca
      541 tcaagcccga catttcgctg cgcttcgtcg atccggacgc agacccggcg gcaacgcgcg
      601 ccgccggcat cagcatcgac ggcgaattgg cggtgaccta caagcagcat gccgaacagt
      661 tgaaagtgct gaccgagcgc gagttcgaca acgccttggt gcggctggcg cgcacccgcg
      721 tgcgcctggt cgcgttcctc gaaggcgatg gcgagcgcaa acccgatggc aaggcgaatg
      781 cggacttggg caacttcggc gcgctgctcg ccgagcaggg cgtgcgcacg ctggatttga
      841 cgctgagcga cggcatgcac gttccgcaga acgtcgatgt gctggtgctc gcgagcccgc
      901 aggtagcgct ggcgccggcg ctggtggacg agctggccga ttacctcgcg cgcggcggca
      961 atttcctgtg gctgaccgag ccgggcgcga atgcggggct ggacgcgctc gccaaggcgc
     1021 tgtcgctgcg cgtgctcgat ggcaccgtgg tcgacggtgc gggcgtggcg ctgggtctgg
     1081 gcgatccgag ttttgtggcg atttccacgt atccacaaaa cagcatcacg cgcgatttcg
     1141 cgctgacc
//
LOCUS       DASRVO010000529         1581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_275693, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000529 DASRVO010000000
VERSION     DASRVO010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275693"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VFF05_06570"
     CDS             <1..322
                     /locus_tag="VFF05_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZW51504.1"
                     /translation="GIDPEHPQRGAEPARSGAVPTAPGKAPKPLLYLQVGAFADPANA
                     ERAAAKVRAAHLGSVAVVAAQIGGKTLRRVRLGPLRDAAEADALARRLKALGLGEPQV
                     SVDN"
     gene            440..>1581
                     /locus_tag="VFF05_06575"
     CDS             440..>1581
                     /locus_tag="VFF05_06575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZW51505.1"
                     /translation="MAAPHKHKKAAPPAAQPAVQPTEQATPSPAEMPTPNPTPRAAPG
                     ALNVPTPPPPTLDAKSWVLMDYATGQILAQNNADERVQPASITKMMTGYIVSAELAAG
                     KIKLDDPVLISEHAWREGGAGTDGSTTFLKVNSQVPLKDLLYGMIIQSGNDAAIALAE
                     HVAGSEDTFANLMNEYATKLGMTGTHFVNATGLPDPNHYSTAHDIATLARRVIQDYPN
                     EYKIYAIKEFEWNGIVQHNRNALLWRDASVDGLKTGHTKEAGFCLTTSAKRGDQRLIA
                     VVMGTPSDKQRADDNQTLLNYGFRFFETHKLYAADKPLTSPELWKGAQNTLALGVAQD
                     VLVTLPRGRYNDLKASLKLPGRLVAPYQKGQPVGTLSVSLDGKTLL"
BASE COUNT          326 a          543 c          481 g          231 t
ORIGIN      
        1 tggcatcgat cccgaacatc cgcagcgcgg cgcggaaccc gcgcgcagcg gcgctgtccc
       61 aactgcgccc ggaaaagcgc ccaaacccct gctatacctg caggtgggcg cgttcgcgga
      121 cccggccaac gcggagcgcg cggcggccaa ggtgcgcgcc gcacatctgg gcagtgtcgc
      181 ggtggtcgcg gcgcagatcg gcggcaaaac cctgcgccgc gtgcgcctgg ggccactgcg
      241 cgatgccgcc gaagcggacg cgctcgcccg cagactcaag gccctggggt tgggcgagcc
      301 gcaggtttca gtggataatt gacgatccga ttcgccgggg atggatcgag aaccgaaaac
      361 tacggatgta atgatgaaac gtttgtacta ttttgctttt gctgcattgt ttgcattttc
      421 cacaatggcc acgccggcca tggccgctcc gcacaaacac aagaaggctg cgccgcccgc
      481 cgcacaaccg gcggtgcaac cgaccgagca ggccacaccc tcgcccgccg aaatgccgac
      541 gccgaatccg acgccgcgcg ccgcgcccgg tgcactgaac gtgcccacgc cgccgccacc
      601 gacgctcgat gcgaaaagct gggtgctgat ggattacgcg acaggccaga ttctcgcgca
      661 gaacaatgcc gacgagcgcg tgcaaccggc atcgatcacc aagatgatga ccggctacat
      721 cgtttcggcg gaactcgccg ccggcaagat caagctcgac gatccggtgc tgatcagcga
      781 acacgcctgg cgcgaaggcg gcgccggcac cgacggatcg accaccttcc tgaaagtgaa
      841 ctcgcaggtg ccgctcaagg atctgctcta cggcatgatc atccagtccg gcaatgatgc
      901 cgcgatcgca ctcgccgagc acgtcgccgg ttccgaagac acgtttgcga acctgatgaa
      961 cgaatacgcg accaagcttg gcatgaccgg aacgcatttc gtcaacgcga ccggcctgcc
     1021 ggatccgaac cactattcca ccgcgcacga catcgccacg ctcgcgcggc gcgtcattca
     1081 ggactatccg aacgaataca agatctacgc gatcaaggaa ttcgagtgga acggcatcgt
     1141 ccagcacaac cgcaacgcgc tgttgtggcg cgatgcgagc gtcgacggcc tgaaaaccgg
     1201 ccacaccaag gaagccggtt tctgcctgac cacgtctgcc aagcgcggcg accagcgcct
     1261 gatcgcggtg gtcatgggca cgcccagcga caagcagcgt gcggatgaca accagaccct
     1321 gctcaactac ggcttccgct tcttcgagac gcacaagctc tacgccgccg acaagccgct
     1381 gacctcgccg gaattgtgga agggcgcgca gaacacgctg gcgctcggcg tcgcgcagga
     1441 cgtgctcgtg accctgccgc gcggacgcta caacgatctc aaggccagcc tgaaactgcc
     1501 cgggcgcctg gtcgcgccgt accagaaagg ccagccggtc ggtacgctgt ccgtctcgct
     1561 ggacggcaag accctgctcg a
//
LOCUS       DASRVO010000530         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_42429, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000530 DASRVO010000000
VERSION     DASRVO010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42429"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..961
                     /gene="phhA"
                     /locus_tag="VFF05_06580"
     CDS             71..961
                     /gene="phhA"
                     /locus_tag="VFF05_06580"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017158567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HZW51506.1"
                     /translation="MNAQPRRVEHLQTDKGNVPVYTTAVVEQPWASYAREDHATWAAL
                     FERQQEILVGRAAREFLDNQRKFGMTPDAIPKFDDLNRVLRKTTGWELVGVEGLLPEL
                     TFFEHLANRRFPVTWWIRRPDQIDYISEPDLFHDLFGHVPLLLNREFADYMQAYGCGG
                     VKAHGIGADALVNLTRLYWYTVEFGLIKQDDGLRIYGSGIVSSKGESIHCLESSAPNR
                     LGFDLKRIMRTRYRIDTYQKTYFVIDSYQQLMDATAPDFTPIYAELARQESIPAGNIL
                     PSDFVYNRGTGEGWASDGDI"
     gene            complement(988..2253)
                     /locus_tag="VFF05_06585"
     CDS             complement(988..2253)
                     /locus_tag="VFF05_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HZW51507.1"
                     /translation="MKPWIILLVLVGVAAAAVFGWQALAADPGLLSIRLGRTHIETTL
                     VFAVFVLLAVWILLGLLWRGVRWPLRAWKLRSQRRGRERFAAGLTALAEGDHRRALRA
                     LEKASQHPELHAPALLATARAAHARGDGERAEAALEEASAHAPAAALALRAQFLLEHG
                     NAEAALALLKPSTDKNDLPPVGWRLLAEAALLTGDNALALRAVDALATSDALTPEALV
                     ALRARVLAAALAATPDSEALTAMWSGLPRAQRAMPEAIAAYARRAAALGQPLAAMDEI
                     ESALRKQWSERLAAVWGQLGLAHADTRLARAEGWLSAQPDSPALLRTLGRLCVQGKLW
                     GKAREYLERGLALSPSAPLWETLGDCMTGQGNAANAAVCYRNALRIARGEATQELSDA
                     ARAPLDTRASIAEERDAHGMPRLAIPKES"
     gene            complement(2250..>2868)
                     /locus_tag="VFF05_06590"
     CDS             complement(2250..>2868)
                     /locus_tag="VFF05_06590"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZW51508.1"
                     /translation="AIAAYRLADAALAEVDDAAFSTVRQSIAGEIVALNDLQAADPQA
                     LVMRLSHLRAQVPELTRAGSLPPPAPAAANESMFARLLGTFVRVSHDSDTEQAQTAVR
                     DISLARDLVALDLRAAQAASLARNDAGFRAAVAEARTQVAAAFDTQAPQTAAVLKELD
                     ALASAQLAPPAPGVLGTALRELRNLRATHALAAAKAAVKHDEAKK"
BASE COUNT          484 a          987 c          943 g          454 t
ORIGIN      
        1 ccgcttcgtc gattcgtgtt tgcgctgccg cagtagtagc attgcgcgca acaatgcaat
       61 ctgctttgcc atgaacgccc agccgcgccg cgtcgaacac ctgcaaaccg acaagggcaa
      121 tgtgccggtc tacaccacgg ccgtggtcga gcagccatgg gcaagttatg cgcgcgaaga
      181 ccacgcgacc tgggcggcgc tgttcgagcg ccagcaggaa atcctggtcg gtcgtgccgc
      241 gcgcgaattc ctcgacaacc agcgcaagtt cggcatgacg ccggacgcga tcccgaaatt
      301 cgacgatctc aaccgcgtcc tgagaaagac caccggttgg gaattggtcg gcgtcgaggg
      361 cctgctgccg gaactcacct ttttcgagca cctcgcgaac cggcgcttcc cggtaacctg
      421 gtggatccgc cgcccggacc agatcgacta catcagcgaa cccgatctgt tccacgacct
      481 gttcggccac gtgccgctgc tgctcaaccg ggaattcgcc gactacatgc aggcctatgg
      541 ctgcggtggc gtcaaggcgc acgggatcgg cgcggatgcg ctcgtcaatc tcacgcgtct
      601 gtactggtac acggtcgaat tcggattgat caaacaggac gacggcctgc gcatctacgg
      661 ttccggcatc gtcagctcga agggcgagtc gatccattgc ctggaatcgt ccgcgccgaa
      721 ccgcctcggc ttcgacctca agcgcatcat gcgcacgcgc tatcgcatcg acacctacca
      781 gaaaacctat ttcgtcatcg acagctacca gcagctgatg gatgcgaccg cgccggactt
      841 caccccgatc tacgccgagc ttgcgcggca ggaatcgatt cccgccggga atatcctgcc
      901 aagcgacttt gtatacaacc gcggcacggg cgagggctgg gccagcgacg gcgatatttg
      961 aatcgctcgc tccggcgagc gcgtggatca actctctttt ggaatcgcca gcctcggcat
     1021 cccgtgcgcg tcgcgctctt cggcgatgct cgcgcgcgta tccagcggcg cacgcgctgc
     1081 gtcggacaac tcctgcgtcg cttcgccgcg cgcgatgcgc agcgcgttgc ggtagcacac
     1141 cgccgcgttc gcggcgttgc cctgcccagt catgcaatcg ccgagcgtct cccacagcgg
     1201 tgcggacggc gaaagcgcca ggccgcgttc gaggtattcg cgcgccttgc cccagagctt
     1261 gccctgcacg cacaagcgcc cgagcgtgcg cagcagggca ggactgtccg gctgcgcgct
     1321 gagccagcct tcggcacgcg ccaggcgcgt atccgcatgc gccaggccga gttggcccca
     1381 gaccgcggcg aggcgctcgg accactgctt gcgcaatgcc gattcgatct catccattgc
     1441 cgccagcggc tggccgagcg cggcggcacg gcgcgcatag gctgcgatcg cctcgggcat
     1501 ggcacgctgc gcgcgcggca aacccgacca cattgccgtc aacgcttcac tatccggtgt
     1561 tgccgcaagg gcggcggcga gcacgcgcgc gcgcagcgcg accagggctt cgggggtgag
     1621 cgcgtcgctc gtggcgagcg catccaccgc gcgcagggca agcgcgttgt cgccggtcaa
     1681 caacgcagcc tcggcaagca ggcgccagcc gaccggcggc agatcgttct tgtcggtgga
     1741 aggtttgagc aaggccagcg cggcttcggc attgccgtgc tcaagcagga actgtgcgcg
     1801 cagcgccaat gcagcagccg gagcgtgcgc cgacgcttct tccagtgccg cttcggcgcg
     1861 ctcgccatcg ccgcgcgcgt gtgccgcgcg cgcggtcgcg agcagggccg gcgcatgcag
     1921 ttcgggatgc tgcgaagcct tttccaacgc acgcagggca cgccgatgat cgccttctgc
     1981 cagcgcggtc agtcccgcgg caaagcgttc gcggccacgg cgctggctgc gcagcttcca
     2041 cgcgcgcaac ggccagcgga cgccgcgcca tagcaggcca agcaaaatcc agacggcgag
     2101 caaaacgaaa acggcgaaga ccaatgtggt ttcgatgtgg gtacgaccga gacggatcga
     2161 aagcaatccg gggtcggcgg ccaaagcctg ccaaccgaac acggccgccg ctgccacgcc
     2221 tacgagcacc agcaagatga tccacggttt cactttttcg cctcgtcgtg tttgaccgcc
     2281 gctttcgccg ccgccagcgc gtgcgtcgcg cgcaggttgc gcagttcgcg caacgccgtg
     2341 ccgagtacgc ccggtgccgg tggtgcgagt tgcgccgacg ccagcgcgtc gagctctttc
     2401 aggaccgccg cggtctgcgg cgcctgcgtg tcgaacgcgg cagcgacctg cgtgcgcgct
     2461 tcggcaaccg cggcgcggaa gccggcgtcg ttgcgcgcga gcgaggctgc ctgcgcggcg
     2521 cgcagatcga gcgcgaccag atcacgggcc agggaaatgt cgcgcaccgc ggtctgcgcc
     2581 tgctcggtgt cgctgtcgtg gctcacgcgc acgaacgtgc cgagcaggcg tgcaaacatc
     2641 gattcgttgg cggcggccgg cgctggaggc ggcaagctcc cggcccgcgt cagttccggc
     2701 acttgcgcgc gcaggtgcga aaggcgcatc accagcgctt gcggatcggc ggcttgcaga
     2761 tcgttcagcg cgacgatttc gccggcgatg ctctggcgca cggtggaaaa cgccgcatcg
     2821 tcgacttccg caagggccgc gtcggccagg cgataagcgg cgatagcc
//
LOCUS       DASRVO010000531         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_223658, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000531 DASRVO010000000
VERSION     DASRVO010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223658"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFF05_06595"
     CDS             <1..844
                     /locus_tag="VFF05_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW51509.1"
                     /translation="TQKAELIGFVVLVRPRATLQPNRETFDLLRIVARQSATHVAEQR
                     MAQALAEVQQLSDYSRRFAFVVHDMKNVASQLGMIVQNARVHRDDPEFQQDVLTTVGS
                     AFERMSHLLARLRPSLPPAADGLIAPSDLIGDEVAALCRSRGAAIEIETDGGAAAVAM
                     DEAGFRSVIRHLCENALEASREHVVVRLRHGALRLEIDIADDGPGMDAEFVRDRLFQP
                     LGSGKRDGFGIGAYQARDLVRAAGGDLLVITRPNLGTTMRIILPCVGKRAQAAPQLAT
                     RAAQ"
     gene            841..>1040
                     /locus_tag="VFF05_06600"
     CDS             841..>1040
                     /locus_tag="VFF05_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZW51510.1"
                     /translation="MTKTKLLIVEDDDGLARQYRWALPDYQLFFASTRQKAVAIAARE
                     HPPVALIDLGLPPDPDGSSEGLA"
BASE COUNT          179 a          317 c          350 g          194 t
ORIGIN      
        1 gacacagaag gcggagttga tcgggtttgt cgtgctggtg cggccgcgtg ccacgctgca
       61 gccgaaccgc gaaaccttcg atctgctgcg gatcgtcgcg cgccaatcgg caacccatgt
      121 cgccgagcag cgcatggcac aagccttggc cgaggtgcag cagctaagcg attacagccg
      181 gcgctttgcg ttcgtcgtgc acgacatgaa gaatgtcgcc agccagctcg gaatgatcgt
      241 acaaaatgcg cgcgtccatc gcgacgaccc ggagtttcag caggacgtgc tgacgacggt
      301 gggtagcgcc tttgagcgga tgagccactt gctggcgcgt ttgcgaccga gcctgccgcc
      361 ggcagccgat ggtctgattg ctccgtccga tctgattggc gacgaggttg ccgccttgtg
      421 ccggtcgcgc ggcgcggcga tcgagatcga gaccgacggc ggcgccgccg ccgtcgcaat
      481 ggatgaagcc ggatttcgtt cggtgattcg ccatttgtgc gaaaacgccc tcgaagcctc
      541 gcgtgaacac gtggttgtgc gtctgcgaca cggtgcgctg cgcttggaga tcgacattgc
      601 cgacgatggc cccggtatgg atgcggaatt tgtgcgtgac cgattgtttc aaccgttggg
      661 ctccgggaag cgcgatggct ttgggatcgg cgcctatcag gcccgcgacc tcgtcagggc
      721 cgccggcggc gatctcctgg tgatcacgcg accgaacctc gggacgacga tgcgcatcat
      781 cctgccctgc gtcggcaaac gcgcccaagc cgcgccacaa ttggcgacga gggctgcaca
      841 atgacgaaaa caaagctcct gatcgtcgaa gatgacgacg ggttggcccg gcaatatcgc
      901 tgggccctgc cggattatca gctcttcttt gcgtcgacgc ggcagaaggc ggtcgcgatt
      961 gccgcccgtg agcatccgcc ggtggcgctg atcgacctgg gtttgccgcc cgatcccgac
     1021 ggttccagcg aggggttggc
//
LOCUS       DASRVO010000532         1332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_42557, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000532 DASRVO010000000
VERSION     DASRVO010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42557"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFF05_06605"
     CDS             complement(<1..194)
                     /locus_tag="VFF05_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZW51511.1"
                     /translation="MNVIDIEALAIAALRARIDDNFLRACRLMFECPGRVIVSGMGKS
                     GHIARKIAATLASTGTPAFFV"
     gene            474..719
                     /locus_tag="VFF05_06610"
     CDS             474..719
                     /locus_tag="VFF05_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HZW51512.1"
                     /translation="MDTRSIQTLIEQGLPGATARVEGDDGVHFEATVVCAAFAGKLPL
                     ARHRMVYATLGELMGGEIHALSLRTLTPEEAAKQGMS"
     gene            719..>1332
                     /locus_tag="VFF05_06615"
     CDS             719..>1332
                     /locus_tag="VFF05_06615"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZW51513.1"
                     /translation="MAKIVISGGEPLHGDVWISGAKNAVLPILAATLLADEPVSIGNV
                     PHLHDVTTTMELLGQMGVSLTIDERMKIHVDPRTANRYFAPYDLVKTMRASILVLGPL
                     VARFGEADVSLPGGCAIGSRPVDQHIRGLEALGATVSVENGYIKARAKRLRGARIVMD
                     IVTVTGTENIMMAAALAQGTTIIENAAQEPEVVDLAHCINAMGG"
BASE COUNT          243 a          427 c          449 g          213 t
ORIGIN      
        1 acgaagaacg ccggcgtgcc ggtcgaggcg agcgtggcgg cgatcttgcg cgcgatgtgg
       61 ccggatttgc ccatgcccga gacaatgacc ctaccgggac actcgaacat cagccggcag
      121 gcgcgcagga agttgtcgtc gatgcgtgcg cgcagtgccg cgatcgcgag cgcttcgatg
      181 tcgatcacgt tcatggcgct gcgcttgagc gtcgccgcat cgagcaacgg gttgctcagc
      241 ggattctcgg caagcgacac aaggcgggca ttcatggcgt ggggaccggg cgggaaggcg
      301 ggattgagca gggaaacggc gggatcgtcc cgctgcgggt atctgcggcc aaggattttg
      361 agtaccatgc gcaaccttcg cttattgtaa ccgacggacc ccgtcccggc gaagacgatt
      421 cagggtactg ccgcgtcgca gccgcccgca tcccgccatt ttccgagccg cgcatggaca
      481 cccgcagcat ccagactttg atcgaacaag gcctgcccgg cgccacggca cgggttgaag
      541 gcgacgatgg cgtgcatttc gaggccaccg tggtttgcgc cgcgttcgcc ggcaagctgc
      601 cgctggcacg gcaccgcatg gtgtacgcga cactgggcga actgatgggc ggcgagatcc
      661 atgccttgtc gctgcgcacg ttgacgccgg aagaagcggc aaagcaagga atgagttgat
      721 ggcaaagatc gtgatttcag ggggcgagcc cctccacggc gacgtgtgga tttccggcgc
      781 caagaacgcg gtgctgccca tcctcgcggc aaccttgctc gccgacgagc cggtcagcat
      841 cggcaacgtg ccgcatctgc acgacgtcac cacgacgatg gaactgctcg gccagatggg
      901 cgtgtcgttg accatcgacg agcgcatgaa gatccacgtc gatccgcgca cggccaaccg
      961 ctatttcgcg ccgtacgacc tggtcaagac catgcgcgcg tccatcctcg tgctcggccc
     1021 gctggtcgcg cgcttcggcg aggccgatgt ttccctgccc ggcggctgcg cgatcggctc
     1081 gcgcccggtg gaccagcaca tccgcggact ggaagcgctc ggcgcgacgg tttcggtgga
     1141 gaacggctac atcaaggccc gcgccaagcg cctgcgcggc gcgcgcatcg tcatggacat
     1201 cgtcaccgtc accggcaccg aaaacatcat gatggcggcg gcgctcgcgc agggcaccac
     1261 catcatcgag aacgcggcgc aggagcccga agtggtggat ctcgcgcatt gcatcaacgc
     1321 gatgggtggc aa
//
LOCUS       DASRVO010000533         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_275900, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000533 DASRVO010000000
VERSION     DASRVO010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275900"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFF05_06620"
     CDS             complement(<1..254)
                     /locus_tag="VFF05_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51514.1"
                     /translation="MARRKKKSSLRWPMAIVLAIAIGVAVWVGRARLPALHLPSPASA
                     PGGVLAIDATHSDAANDGRRVRVTGLLAADGPVRDAQLGV"
     gene            complement(255..1118)
                     /locus_tag="VFF05_06625"
     CDS             complement(255..1118)
                     /locus_tag="VFF05_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZW51515.1"
                     /translation="MKLWSILGNSQRLDGGAMFGNAPRAMWEKWIVPDPDNRIPLACR
                     ALLAQDLDGKNVLFETGIGAFFEPKLRERYGVVEDNHVLLDSLRAAGFGHEDIDAVVL
                     SHLHFDHAGGLLAPWHAGAAPELLFPKAKFVVGKRAFERAVHPHPRDRASFIPELQPL
                     LEKSGRLELVENAHSFALGKSVRFTFSDGHTPGLMLAEIVVPSGGVVFCADLIPGRPW
                     VHLPVTMGYDRAPEMLIDEKRAFLEDKLARNVRLFFTHDVQCALARVTRDEKGRFGTS
                     DELPELTGLAA"
     gene            complement(1115..2785)
                     /gene="ggt"
                     /locus_tag="VFF05_06630"
     CDS             complement(1115..2785)
                     /gene="ggt"
                     /locus_tag="VFF05_06630"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HZW51516.1"
                     /translation="MKKRYIAAFVAFAALAFGANAAERPGHAAIASAHKLATEAGFEV
                     LAEGGNAFDAAIAVASTLSVVEPQSSGIGGGGFFLLHRASDDKNVMLDARETAPAAVK
                     ESQYLKSDGTPNRDTALYGPLAAGIPGEPAALVRLATHYGILPLAKSLAPAIRIAREG
                     FQPGKVFLGAIADHREQIARYPGTQALLMPNGEVPKAGWTFRNPDLATTLERIAAHGN
                     DGFYQGKTAELMLKAVRKTGGDWTAADLANYKVVEREPLDFDYRGFRIVTASPPSSGG
                     VALEEMFNVLSGYDLGKFERPQRLHYIIEAMRRAFRDHNEYLGDPDFVKMPLATLLSP
                     QYAAGLRASILPDKATPSSMLPSSVAHDPGMHTTHFSIVDADGNMVATTQTVNTHFGS
                     CLVPPGTGVFLNNEMDDFALVPGAPNAYGLLGNKANSPAPGKRMLSSMTPTFVFGPER
                     VGVIGSPGGSTIITQVFEGILAFMHGESAAQIAATPRYHHQYLPDVVYVEPNAFDADT
                     AKALTGMGYDVKARGHWGFMNVVTWDLHANKLDAASDPRHDAGLGEVQ"
     gene            complement(2782..3039)
                     /locus_tag="VFF05_06635"
     CDS             complement(2782..3039)
                     /locus_tag="VFF05_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HZW51517.1"
                     /translation="MALKILDTCVNCDVCEPVCPNKAIAAGVRIYEIAPESCTECVGH
                     YDAPQCVEVCPVECIIVDADHVESPDALLAKYRALTGGEPK"
BASE COUNT          543 a         1048 c         1047 g          564 t
ORIGIN      
        1 ctcacgccca actgcgcgtc gcgcaccggg ccgtcggccg cgagcaggcc ggtcacgcgc
       61 acgcggcggc catcgttggc ggcatcggag tgtgtcgcgt cgatggcgag cacgccgccg
      121 ggcgcgctgg ccggcgacgg caggtgcaat gccggcagac gtgcgcgccc gacccaaaca
      181 gccacgccaa tggcgatggc gagcacgatc gccatcggcc agcgcagcga ggattttttc
      241 ttgcgccgtg ccattcaggc cgccagaccg gtcagttccg gcagctcgtc gctggtgccg
      301 aagcggcctt tttcatcgcg cgtcacgcgc gccagcgcgc attgcacatc gtgggtgaaa
      361 aacaggcgca cattgcgggc gagcttgtct tcgaggaagg cgcgcttctc gtcgatcagc
      421 atttccggtg cgcggtcgta gcccatcgtc accggcagat gcacccacgg gcggccggga
      481 atcaggtccg cgcaaaaaac cacgccgccg gacgggacga cgatttccgc cagcatcagc
      541 cccggcgtgt ggccatcgct gaaggtgaag cgcacgctct tgcccaaggc gaaggaatgc
      601 gcgttttcca cgagttccag gcgcccggac ttttccagca gcggttgcaa ctcggggatg
      661 aacgaagcgc ggtcgcgcgg atgcggatgc acggcgcgct cgaacgcgcg cttgccgacc
      721 acgaatttcg ccttcgggaa cagcagctcc ggcgccgcgc ctgcgtgcca cggcgcgagc
      781 aggccgccgg catggtcgaa atgcaggtgc gagagcacga ccgcgtcgat gtcctcgtgc
      841 ccgaagcccg ccgcgcgcag cgaatcgagc agcacgtgat tatcctcgac cacgccataa
      901 cgctcgcgca gcttcggctc gaaaaacgcg ccgatgccgg tttcgaacag cacgtttttg
      961 ccgtccagat cctgcgcgag cagcgcgcgg catgcgagcg gaatgcggtt gtccgggtcg
     1021 gggacaatcc atttttccca catcgcgcgc ggcgcgttgc cgaacatggc gccgccatcg
     1081 aggcgctgcg aattgccgag aatcgaccag agcttcattg cacttcgccc aagccggcgt
     1141 catggcgcgg atcgctggcc gcatcgagct tgttcgcgtg caggtcccag gtcaccacgt
     1201 tcatgaagcc ccagtgcccg cgcgccttga cgtcgtagcc catgccggtc agtgccttgg
     1261 ccgtgtccgc atcgaaggca ttcggctcga cgtagacgac gtcgggcaga tattgatggt
     1321 ggtagcgcgg cgtcgccgcg atttgcgcgg cggattcgcc gtgcatgaag gcgaggatgc
     1381 cttcgaagac ctgggtgatg atggtcgatc cgcccggcga gccgatcacg ccgacacgtt
     1441 ccggaccgaa cacgaatgtt ggcgtcatcg aggagagcat gcgcttgccc ggcgcgggcg
     1501 agttcgcctt gttgccgagc aggccgtagg cgttcggcgc accggggaca agggcgaaat
     1561 cgtccatctc attgttgagg aacacacccg ttcccggcgg caccaggcac gagccgaaat
     1621 gcgtgttcac ggtttgcgtg gtcgcgacca tgttgccgtc ggcgtcgacg atggaaaagt
     1681 gcgtggtgtg catgcccgga tcgtgcgcca cggacgatgg cagcatcgaa gatggcgtcg
     1741 ccttgtccgg caggatgctc gcgcgcagtc cggccgcgta ttgcggcgac agcagtgtcg
     1801 ccagcggcat cttcacgaaa tccggatcgc ccaggtattc gttgtggtcg cggaacgcgc
     1861 ggcgcatcgc ttcgatgatg taatgcaggc gctgcgggcg ctcgaacttg cccaggtcgt
     1921 agccggacaa cacgttgaac atttcctcca gcgccacgcc gccggacgaa ggcggcgaag
     1981 cggtgacgat cctgaagccg cggtagtcga aatccagcgg ctcgcgttcg acgaccttgt
     2041 aattggcaag gtcggccgcc gtccagtcgc cgccagtctt gcgcacggcc ttgagcatca
     2101 gttcggcggt cttgccctgg tagaagccat cgttgccgtg agccgcgatg cgttcgagcg
     2161 tcgttgccaa atccggattg cggaacgtcc agcccgcttt cggcacctcg ccgttcggca
     2221 tcagcagcgc ctgcgtgccc ggataacgcg cgatctgttc gcgatggtcg gcgatggcgc
     2281 cgagaaacac cttgccgggc tggaatccct cgcgcgcgat gcggatcgcg ggtgccagcg
     2341 acttcgccag cggcaatatg ccgtaatgcg tagccagcct gaccagcgcc gcgggttcgc
     2401 cgggaatgcc ggccgcgagc ggtccgtaca gcgcggtgtc gcggttcggc gtgccatcgg
     2461 atttgaggta ttgcgattcc ttcaccgccg ccggcgcggt ttcgcgcgcg tcgagcatca
     2521 cgttcttgtc gtcactcgcg cgatgcagca ggaaaaaacc gccgccgccg atgccggaac
     2581 tttgcggctc gaccaccgac aaggtcgacg ccaccgcgat cgcggcgtcg aacgcgttgc
     2641 cgccctcggc cagcacctcg aatccggcct cggtggcgag cttgtgcgcg ctggcgatgg
     2701 cggcgtggcc gggacgctcg gcggcattcg cgccgaaagc cagtgcggcg aatgcgacaa
     2761 atgcggcaat gtaccgtttt ttcattttgg ctctccgccg gtcagcgccc ggtatttcgc
     2821 cagcaatgcg tccggcgatt caacatggtc ggcatcgacg atgatgcact cgaccggaca
     2881 cacttccacg cattgcggcg catcgtagtg gccgacgcac tcggtgcagc tctccggcgc
     2941 gatctcgtaa atccgcacac ctgccgcgat cgccttgttc ggacaaaccg gttcgcagac
     3001 gtcgcaattg acgcaggtgt cgaggatctt caaagccatc gcaatttccc tctgccgttg
     3061 gcgacagagg ggaatattcc cgcgttactt catctccacg aaccgcaact gtgcgcccga
     3121 tttcgcgatt gcgtccttga cccgttgttc gtccgcgctg gcgccggcga acagcaccac
     3181 cacgtccttg aaggagccat cg
//
LOCUS       DASRVO010000534         1121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_42602, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000534 DASRVO010000000
VERSION     DASRVO010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42602"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFF05_06640"
     CDS             <1..522
                     /locus_tag="VFF05_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZW51518.1"
                     /translation="LPSGSCGGMIRVHYPDLFRDDPELHQRFAALGERTYELTDFLVN
                     IARLEQAPGSYSGAITYHDSCSGLRELDVYRQPRALLATMPGVTLHEMVESTTCCGFG
                     GTFSVKYGELSTRMADNKCAHITDTHAGTIVGGDLGCLLNIEGRLRRNGDHTTRVLHV
                     AEVLSGIDSDGTE"
     gene            522..>1121
                     /locus_tag="VFF05_06645"
     CDS             522..>1121
                     /locus_tag="VFF05_06645"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HZW51519.1"
                     /translation="MQNTSLQFKKQVTIKLADVRLQDALSRLQSRFVGGRSAVIGEID
                     DLQATRTAAAAIRDRVLNDLEHWLLHFEQQASARGAIVHWAETGEDVNRIVAEIAAQY
                     GVKTAAKSKSMVSEECGLNDALELAGVKVMETDLGEYILQLAHEAPSHIVAPVVHKNV
                     DEIADLFAEKHHKPRKTDIAELCREAREILRPAFLGADMG"
BASE COUNT          227 a          355 c          347 g          192 t
ORIGIN      
        1 ctgccatcgg gttcgtgtgg cgggatgatc cgcgtgcact accccgacct gtttcgcgat
       61 gatcccgagt tgcaccaacg attcgccgcc ctgggcgagc gcacctacga actgaccgat
      121 ttcctggtca atatcgccag gctggaacaa gcacccggca gctacagtgg ggctatcacc
      181 tatcacgatt cctgctccgg gttgcgcgag ttggatgtct accggcagcc gcgcgcgctg
      241 ttggcgacga tgcccggcgt cacgctgcac gagatggtgg aatcgaccac ctgctgcggc
      301 ttcggcggca cgttctcggt caagtacggc gaactgtcga cgcgcatggc ggataacaaa
      361 tgcgcgcata tcaccgacac ccatgccggc accatcgtcg gcggcgacct cggctgcctg
      421 ctcaatatcg aaggccgctt gcgccgcaac ggcgatcaca ccacccgcgt gttgcacgtg
      481 gcagaggtgc tgtccggcat cgacagcgac gggacggaat aatgcaaaac acttcgctgc
      541 aattcaagaa acaggtcacc atcaaactgg ccgatgttcg gttgcaggac gccctgtcgc
      601 gcctgcagtc gcgttttgtc ggcgggcgct ccgcggtgat tggcgagatc gacgatctgc
      661 aggcgacgcg caccgccgcc gccgcgatcc gcgaccgcgt gctgaacgat cttgaacact
      721 ggctgctgca ttttgaacaa caggccagcg cgcgcggcgc gatcgtgcac tgggccgaaa
      781 ccggcgagga cgtgaaccgc atcgttgccg aaatcgccgc gcaatatggc gtcaagaccg
      841 cggcaaagtc caagtccatg gtcagcgagg aatgcgggct gaacgatgcg ctggaactgg
      901 ccggcgtcaa ggtgatggaa accgacctgg gtgaatacat cctgcaactc gcgcacgaag
      961 caccctcgca catcgtcgcg ccggtggtgc acaagaacgt ggatgagatc gccgacctgt
     1021 ttgccgagaa acatcacaaa ccgcgcaaga cggacatcgc cgaactgtgc cgcgaggccc
     1081 gtgaaatatt gcggccggcc ttccttggtg ccgacatggg c
//
LOCUS       DASRVO010000535         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_223802, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000535 DASRVO010000000
VERSION     DASRVO010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223802"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1403
                     /locus_tag="VFF05_06650"
     CDS             231..1403
                     /locus_tag="VFF05_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZW51520.1"
                     /translation="MPAAALNPVDLYDVRSLLSEEERMVQDTVARFTDERVLPVIGDC
                     FDKGRFPTELIPEMAELGLLGATLPHEYGCAGMNGVSYGLICQELERGDSGIRSFASV
                     QSSLCMYPIYAYGSEEQRKHWLPQMAAGKVIGCFGLTEPHGGSDPANMKTNAKKDGGD
                     WVINGAKMWITNGNLAQIAIVWAQTDEGIQGFIVPTDSKGFTAQEIHKKMSLRASVTS
                     ALFFDNVRVPESNRLPNVKGLKGPLGCLTQARYGITWGPIGAAIACFTETTEYAKQRI
                     LFKRPLAANQAVQLKLADMARRITLAQLLSLQLGRLKDAGAMQPTQVSLAKWNNVRMA
                     IDIARECRDILGGAGITTEHCPIRHALNLESVITYEGTETVHQLVVGREVTGINAF"
     gene            1414..1959
                     /locus_tag="VFF05_06655"
     CDS             1414..1959
                     /locus_tag="VFF05_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZW51521.1"
                     /translation="MDQPRIETARLILRLPRIEDFDGYAELHADEEAARHIGGALPRA
                     AAWRKFLQMPGAWMVQGYGMFSVCDKASGEWLGQCGPWFPEGWPGTEVGWAFRRSAWG
                     KGYAHEAAVATIDWAFANLGWTEVIHSISPENTPSQALARRLGSRLLRQSPLPEPYAD
                     HIVEVWGQSREEWLSRRVAAA"
     gene            1981..>2200
                     /locus_tag="VFF05_06660"
     CDS             1981..>2200
                     /locus_tag="VFF05_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51522.1"
                     /translation="MNAVANPIPARHKSLNRAEVCDQNFVEFVQDWSGPVTQVPPADA
                     PVLTGSLCSVRDFVELFDSQLISRHLDLM"
BASE COUNT          403 a          685 c          714 g          398 t
ORIGIN      
        1 ttgatcgacg acccaatgca cgggcacgat gcgcaggccc atgtcgtcga tcaggcgcag
       61 gtcggctttg agggcgacat ggtcgttgtt cgcgtagtcg ttgccggcga tgttgacggc
      121 gtggtaggtg aggacgggga ctcggaacac ttgatttggc gggactttcg ggtgtcgggt
      181 cgctagaata cgcggccttg ccgcccgttc gcaaaacccg gagtttttcc atgcccgccg
      241 ccgcgctcaa tcccgtcgat ttgtacgatg tccgctcgct gctcagcgag gaagagcgca
      301 tggtgcagga taccgtcgcg cgcttcacgg acgagcgcgt gctgccggtc atcggcgatt
      361 gtttcgacaa gggtcgattc ccgacggagt tgatcccgga aatggccgaa ctcggcctgc
      421 ttggcgccac gctcccgcac gaatacggtt gcgccggcat gaacggcgtc agctacggct
      481 tgatctgcca ggaactcgag cgcggcgatt ccggcatccg cagtttcgct tcggtgcaat
      541 cgtcgctgtg catgtatccg atctacgcct acggcagcga ggagcagcgc aagcattggc
      601 tgccgcagat ggcggccggc aaggtgatcg gctgcttcgg tttgaccgaa ccgcacggcg
      661 gctcggatcc ggccaacatg aagacgaacg cgaagaaaga cggcggcgac tgggtcatca
      721 acggcgccaa gatgtggatc acgaacggta acttggcgca gatcgccatc gtctgggcgc
      781 agaccgacga gggcatccag ggcttcatcg tgccaacgga ttccaagggc ttcaccgcgc
      841 aggaaatcca caagaaaatg agcctgcgcg catcggtcac gtccgcgctg ttcttcgaca
      901 acgtgcgcgt accggaatcg aaccgcctgc cgaacgtgaa gggcctgaaa ggtccgcttg
      961 gctgcctcac ccaggcccga tacggcatca cctggggtcc gatcggcgcg gcgatcgcct
     1021 gcttcacgga aaccaccgag tacgccaaac aaagaatcct gttcaagcgt ccgctcgctg
     1081 ccaatcaggc ggtgcagcta aagcttgccg acatggcgcg ccggattacg ctggcacagc
     1141 ttctgtcgct gcagcttggc cgcctgaagg atgccggcgc gatgcagccg acgcaggtgt
     1201 cgctggccaa gtggaacaac gtgcgcatgg ccatcgacat cgcgcgcgaa tgccgcgaca
     1261 tcctcggcgg cgccggcata acgaccgagc actgtccgat ccggcatgcg ctcaaccttg
     1321 aatccgtcat cacctacgaa ggtaccgaaa ccgtgcatca actcgtcgtc ggacgcgagg
     1381 tgacgggaat caatgcattc tgatcgcccc aacatggatc agccacgcat cgaaaccgcg
     1441 cgcctgatcc tgcgcctgcc gcgcatcgag gacttcgacg gctacgccga attgcatgcc
     1501 gacgaggaag ccgcgcgcca tatcggcggc gcgctgccgc gtgcggcggc gtggcgcaaa
     1561 ttcctgcaga tgcccggcgc gtggatggtg cagggctacg gcatgttctc ggtgtgcgac
     1621 aaggcgagcg gcgagtggct cggacaatgc gggccgtggt ttccggaagg ctggcccgga
     1681 accgaggttg gctgggcgtt ccggcgcagc gcctggggca agggctatgc gcacgaggcc
     1741 gccgtcgcga ccatcgactg ggcgttcgcg aacctgggct ggaccgaggt catccattcg
     1801 atcagtccgg aaaacacgcc ttcgcaggcc ctggctcggc ggctcggctc gcgcctgctg
     1861 cgccagtcgc cgttgccaga gccctacgcc gaccacattg tcgaggtctg gggccagtcg
     1921 cgcgaggaat ggctttcccg ccgcgttgcc gcggcatgaa ttcctgagtt gaacgatgcc
     1981 atgaacgcag tcgccaatcc cattcccgcc cgccacaaga gtcttaatcg cgccgaagtc
     2041 tgcgatcaga actttgtcga gttcgtgcag gattggtccg gcccggtcac gcaggtgccg
     2101 cctgccgatg cacccgtgct gactggcagc ctgtgcagcg tgcgtgattt cgtcgagttg
     2161 ttcgattcgc agttgatatc gcggcatctg gatctgatgg
//
LOCUS       DASRVO010000536         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_352783, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000536 DASRVO010000000
VERSION     DASRVO010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352783"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..941
                     /locus_tag="VFF05_06665"
     CDS             102..941
                     /locus_tag="VFF05_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZW51523.1"
                     /translation="MTFRIAVVQPIANPIGEDERNVADAVKFIARAKAEGADFVCFPE
                     TYPGPWRMPASFDPTPRIAEAAAKHGVNAVYGTLEPISHNEGTAYNLICMAYGDGRAP
                     VSYRRTHPNGPWIYTGGKWWEFQYVAGNEFPVFDTQHGKVGLAMCSEAYMPEVTRALA
                     LRGAELIFMPAGTDKGKLWASWRNLIWSRAIENLALVVTTQNLFSHAERGLAMVAAPE
                     EILFESTAAGFYVVEVSLERAREMRSTADSVTSSQQYGAKQGVLGPQWQRPELRETMY
                     PRA"
BASE COUNT          186 a          328 c          338 g          163 t
ORIGIN      
        1 agcctgccat gcgggacggc tttgtcgccg cgttgcggcg cgcgcttcaa atcgcagccg
       61 ttcgcgatta agctcggcgc ccgcaacagg gagtgacgcg catgaccttc cgcattgccg
      121 tggtgcagcc gatcgccaac ccgatcggcg aggatgagag gaacgtcgcc gacgcggtca
      181 agttcatcgc gcgcgccaag gcggaaggcg ccgatttcgt ctgcttcccg gaaacctatc
      241 cggggccgtg gcgcatgccg gccagtttcg atccgacgcc gcgcattgcg gaagccgccg
      301 ccaagcacgg cgtcaacgcc gtctacggca cgctggagcc gatcagccac aacgaaggca
      361 ccgcctataa tctcatctgc atggcctatg gcgacggccg cgcgccggtg tcttatcgcc
      421 gcacgcatcc gaacgggcca tggatctata ccggcggcaa gtggtgggaa ttccaatacg
      481 tggcgggcaa cgagttcccg gtgttcgaca cgcagcacgg caaggtcggg ctcgccatgt
      541 gcagcgaggc ctatatgccg gaggtgacgc gcgcgctggc attgcgcggc gctgaattga
      601 tcttcatgcc agccggcacc gacaaaggaa aactgtgggc gagctggcgc aacctgatct
      661 ggtcgcgcgc catcgagaac ctcgcgctcg tggtgacgac gcagaacctg ttcagccatg
      721 ccgagcgcgg gctcgccatg gtggcagcgc cggaagagat cctgttcgag agcacggcgg
      781 ccggtttcta tgtcgtggag gtgagcctgg agcgcgcgcg cgaaatgcgc agcacggccg
      841 atagcgtcac ctcgtcgcag caatacggcg ccaagcaggg cgtgctcggc ccgcaatggc
      901 agcggccgga attgcgcgag acgatgtatc caagggcgta gtgacgtccc tctccccgct
      961 tctccgcgtc attccggggc gcggacgaag tccgcgagcc cggaatccat aactc
//
LOCUS       DASRVO010000537         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_249414, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000537 DASRVO010000000
VERSION     DASRVO010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249414"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..469)
                     /locus_tag="VFF05_06670"
     CDS             complement(41..469)
                     /locus_tag="VFF05_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZW51524.1"
                     /translation="MRLVKHLLEDKGGAIYAIEPDKPVLDAIRLMAEKYIGALLVMKG
                     SQLLGIVSERDYARKVILKGRSSADTPVREIMSASVLTVAPGDSVNHCMKLMTDRRVR
                     HLPVVDDGRVLGVVSIGDLVKAVIEDQAQELEHMQRYISG"
     gene            complement(553..>2875)
                     /locus_tag="VFF05_06675"
     CDS             complement(553..>2875)
                     /locus_tag="VFF05_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51525.1"
                     /translation="FTMMYAPSAGGYPQYQAIGKVVVDPNNSNNVIAGTKTGLYVSND
                     AGVNWTGPCYTNGYATGSGAQRQDMTGLAAVNIDGTTKLYAAVGTRGTATPVQPDLGN
                     NGANGVYSAVMPASGCPALGAWTLLNSGMPATLGNGTPSATYGRIEIAVAPSDPQTVY
                     AMISDIGSSGIYGIYKTTNGGSSWTKLTNPDGTQPDSGTKVGTQMWYDAGLTVSPIDP
                     NVFFVSTVELLKGTNTSGSTATYVNLTSAYNGGPVHPDNHARAIVGGDPDKVIDGNDG
                     GVYYISNATKATSFLNATWTDMNNGLPTIEVYGGDISANFATSATPKAAAGFQDNGSA
                     YVTYGGTPSTAAWTANNGGDGFYSRIEPVLGTRFYSSVYNGAIYGGAAGGQTSIKSGG
                     WSSDRLNFITTFDIYRYGDTGVAGSGCNTTAGCTHLIYGSQRLWETATATDPTSSSWK
                     VQTGDLTKGTLVIGADNRSYINQLHYSFADPTIAIIGTTDGNVQYVFNLGTGTANAAI
                     AVNVTGGNAVLPNRTISDVSTDPANPLVGYAAVAGFAANTPSTPGHVFQVTCTANCAS
                     FAWADKTGNLPDIPADAIIPNPHDPNQVFVGTDWGLYYTDDISVASPLWQRFEGLPHV
                     MVWSLTIDRGFTTLAAYTRGRGVWAWPLPSGSIGPNHLTISQAPASIVQGSTLGSVTV
                     TEYDAGNQQTADSATQVTLTAGACGGIVLGTGTLNGGAITFDTTTRFRTPAASLSLAA
                     SAGAASPSAASAAFDVVADPDWVFGSSFEGCTP"
BASE COUNT          446 a          901 c          985 g          543 t
ORIGIN      
        1 cacaaagaat ctcgaaacct ctcgaaaccg tcgcgcgcga tcaacccgag atatagcgct
       61 gcatgtgctc cagttcctgc gcctgatcct cgatcaccgc cttgaccaga tcgccgatcg
      121 acaccacgcc gagcacgcgg ccatcgtcga ccaccggtag atggcgcacg cggcggtcgg
      181 tcatgagctt catgcagtga ttgacgctat cgcccggcgc cacggtgagc acggacgcgg
      241 acatgatctc gcgcaccggc gtgtccgccg acgagcggcc tttcaggatc accttgcgcg
      301 cgtagtcgcg ctcggaaacg atgccgagca gttgcgaacc tttcatcacc agtagcgcgc
      361 cgatgtactt ttcggccatc aggcggatcg catcgagcac gggtttgtcc ggttcgatgg
      421 cgtagatcgc gccgcccttg tcttcgagca gatgcttgac gagtcgcata cgtcctccgc
      481 ggcgggatga gacgtaccca ttctagcgct agcgcagcgc ggacggcagt ggccgtccgc
      541 gccggcaaat gcctatggcg tgcagccttc gaagctgctc ccgaacaccc agtcgggatc
      601 cgccacgacg tcgaacgcag ccgatgccgc gctcggcgac gcagcgccag cggacgccgc
      661 caggctcagg ctcgccgccg gagtcctgaa tcgtgtcgtc gtatcgaacg tgatcgcgcc
      721 gccattcagc gtgcctgtgc ccagcacgat gccgccgcag gcacccgccg tgagggtcac
      781 ttgcgtcgcg ctgtccgcgg tttgctgatt gccggcgtcg tactcggtga cggtcacact
      841 gccgagtgta ctgccctgga cgatgctggc cggcgcctgg gagatggtca ggtgattcgg
      901 cccgatcgag cccgatggca gcggccacgc ccacacgccg cggccgcgcg tgtaggcagc
      961 gagcgtggtg aagccgcggt cgatggtgag gctccacacc atcacgtgcg gcagtccttc
     1021 gaagcgctgc cacagcggcg aggcgacgga aatgtcgtcg gtgtagtaca agccccagtc
     1081 ggtgccgacg aacacctgat tcggatcgtg cggattcgga atgatcgcgt cggctggaat
     1141 gtccggcagg ttgccggtct tgtccgccca ggcgaaactc gcgcagttcg ccgtacaggt
     1201 gacctggaac acgtggcccg gcgtggaagg cgtgttcgcg gcaaaccccg cgacagccgc
     1261 gtagccgacc agcgggttgg cgggatcggt gctcacatcc gaaatcgtgc ggttcggcag
     1321 cacggcgttg ccgccggtga cgttgacggc gatggcggcg ttggccgtgc cggttccgag
     1381 attgaacacg tactgcacgt tgccgtcggt ggtgccgatg atggcgatgg tcgggtcggc
     1441 gaatgagtag tgcaactggt tgatgtagga acggttgtcc gcgccgatga cgagggtgcc
     1501 cttggtcagg tcgccggtct ggaccttcca cgacgaactg gtcgggtcgg tggcggtggc
     1561 ggtttcccac aggcgctggc tgccgtagat caggtgcgtg cagcccgcgg tcgtgttgca
     1621 accgctgccg gcgacgccgg tgtcgccgta gcggtagatg tcgaaggtcg tgatgaagtt
     1681 gaggcgatcg ctcgaccagc cgccggactt gatcgaagtc tggccgcccg ccgcgccgcc
     1741 gtaaatcgcg ccgttataca ccgatgaata gaagcgcgtg ccgagcaccg gttcgatgcg
     1801 cgaatagaag ccgtcgccgc cgttgttcgc cgtccacgcg gcggtactgg gcgtgccacc
     1861 gtaggtgacg taggcgctgc cgttgtcctg gaaacccgcg gcagccttgg gcgtggcgga
     1921 cgtggcgaag ttcgcgctga tgtcgccgcc gtaaacctcg atggtcggca gcccgttgtt
     1981 catgtccgtc cacgtcgcgt tcaagaagct cgtcgccttg gtcgcgttgg agatgtagta
     2041 gacgccgccg tcgttgccgt cgatcacctt gtcgggatcg ccgccgacga tggcgcgcgc
     2101 atggttgtcg ggatgcacgg gaccgccgtt gtacgcgctg gtgaggttga cgtaggtggc
     2161 cgtggaaccg ctcgtgttcg tgcctttgag cagttctacg gtgctgacga agaacacgtt
     2221 cggatcgatc ggcgacacgg tcaggcccgc gtcgtaccac atctgcgtgc cgaccttggt
     2281 gccagaatcc ggctgcgtgc cgtcggggtt ggtcagcttc gtccagctcg atccgccatt
     2341 ggtggttttg tagattccgt agatgccgct cgatccgatg tccgagatca tcgcgtacac
     2401 ggtctgcggg tcgctcggcg ccaccgcgat ctcgatgcgg ccgtaagtgg cgctcggcgt
     2461 gccgttgccg agcgtggccg gcatgccact gttgagcagg gtccacgcgc cgagtgcggg
     2521 gcaaccgctc gccggcatga ccgcgctgta gacgccattg gcgccgttgt tgcccagatc
     2581 cggctgcacc ggcgtggcgg tgccgcgcgt gccgacggcg gcgtacagtt tcgtcgtacc
     2641 gtcgatgttg accgccgcaa ggccggtcat gtcctgacgc tgcgcgccgc tgcccgtggc
     2701 gtagccgttg gtgtagcacg gaccggtcca gttcacgccg gcgtcgttgg agacatacag
     2761 tccggtcttg gtgccggcga tgacgttgtt cgagttgttc ggatcgacca cgaccttgcc
     2821 gatcgcctga tactgcgggt agccgccggc ggacggcgcg tacatcatgg tgaac
//
LOCUS       DASRVO010000538         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_276070, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000538 DASRVO010000000
VERSION     DASRVO010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276070"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFF05_06680"
     CDS             complement(<1..713)
                     /locus_tag="VFF05_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZW51526.1"
                     /translation="MTAAVPRAALWRMFAGAALISTTSIFVRWAHVGPTESAFWRMAF
                     GGAMLLVLLLARGQWRGIEWRDWAWLVLPAFAFGADLFIWHRSIEYIGPGLATLVANF
                     QVFIMALAGVLFFRERLGPRFIFGLVLALIGLWLLVGVDWGSFTPQFRLGVWLGLATG
                     VAYAVYLLSLRRAQLVRSGLTPMRALCVNSLLCAAMLAGTVLAEGGSFAIPDGETWAA
                     LLGLGLFGQVLGWVFIASA"
     gene            complement(741..1369)
                     /locus_tag="VFF05_06685"
                     /pseudo
     CDS             complement(741..1369)
                     /locus_tag="VFF05_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010367418.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1378..1644)
                     /locus_tag="VFF05_06690"
     CDS             complement(1378..1644)
                     /locus_tag="VFF05_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HZW51527.1"
                     /translation="MQIVYRAENIIDANLVKNALEAEGIPAFVGGQYLTGAIGELPPM
                     PLVNVMVAAIDWPRARAIAESIDAELAEYRAEPESGAGWLPEPV"
     gene            1738..>3067
                     /locus_tag="VFF05_06695"
     CDS             1738..>3067
                     /locus_tag="VFF05_06695"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HZW51528.1"
                     /translation="MTEISHNFIRQIVLDERAAGKHGGRVGTRFPPEPNGYLHLGHAK
                     SICLNFGIAREFGGSCNLRFDDTNPAKEDVEYVESIKADVQWLGFDWTELRHASDYFE
                     VFYRCAEKLIRDGKAFVCDLSADEVREYRGTLTEPGRNSPFRDRSVDENLDLFRRMRK
                     GEFADGAKTLRAKIEMASGNINLRDPALYRIRKVQHQNTGDAWCIYPMYDYAHCISDA
                     VEGITHSLCTLEFEDHRPLYDWCLAQIDLPNHPELVQPLVDAGFKTNVSTPRQIEFAR
                     GNLDYTVMSKRKLMELVRDGLVAGWDDPRMPTLSGLRRRGFPPAAIRTFWERAGVTKQ
                     NSTIEFSVLEGCVREVLDANAPRRFAVLDPLKLVITNLPDDHAETLTFPNHPKNETFG
                     ARSVAFARELWIEREDFMETPVKGFHRLAPSSEVRLRGVGIVKCTDVQKDA"
BASE COUNT          596 a         1042 c          962 g          467 t
ORIGIN      
        1 atcgcgctgg caatgaacac ccaacccagc acttggccga acaggccgag gccgagcagc
       61 gcggcccagg tttcgccgtc gggaatcgcg aagctgccgc cttcggccag cacggtaccg
      121 gccagcatcg ccgcgcaaag caggctgttc acgcacagcg cgcgcatcgg cgtcaggccg
      181 gaccgcacga gctgcgcgcg gcgcaggctg agcaggtaca cggcatacgc gacgccggtg
      241 gcgagtccaa gccagacgcc gagacgaaac tgcggcgtga aggagcccca gtccacgccg
      301 accagcagcc agagcccgat cagcgccagc acgaggccga agatgaaacg cggacccagg
      361 cgctcgcgga aaaacaacac gccggccagg gccatgatga aaacctggaa attcgcaacc
      421 agtgtcgcga ggccggggcc gatgtactcg atgctgcgat gccagatgaa caggtccgcg
      481 ccgaacgcga acgccggcag cacgagccac gcccagtcgc gccattcgat tccccgccac
      541 tgcccgcgcg cgagcagcag cacaagcaac atcgcgccac cgaaagccat gcgccagaaa
      601 gcggattcgg tgggacccac gtgcgcccag cgtacgaaaa tgctggtcgt gctgatcagc
      661 gcggcacccg cgaacatgcg ccacagcgcg gcgcgcggaa ccgccgccgt cacttggcct
      721 gctcgtgcag cggcgccagc ggctcgttca cggtcgcggc cagccagtcg cgcagcgccg
      781 gcagggcaag gatcgcgtcg gcataggcgc gctcgaccgg atcgagcgcg acgtcgtagc
      841 tttcgaaacg caacacgacc ggtgcgtaca tggcgtcggc gatgccgaac gcgccaaaca
      901 ggaacggccc gcccttgccg tgacgcgcac gcgcttcgtg ccagatcgcc ttgacccgcg
      961 cgatgtcggc ttgggcttcg gcgctggtcg ggtggttctt cacgcgcttg cggcagttca
     1021 tcgacaattc gttgcgcagc gccgcgaatc cggaatgcat ctcggcgctg atcgaacgcg
     1081 ccagcgcgcg cgcggccatg tcggccggcc agccgcggcc gccaagccag cgctcgttcg
     1141 cgtattcggc gatcgccagc gaatcccaca cgcgcacgtc gccgtcgtgc agcaccggca
     1201 cacgcccggt cggcgagtag tcgcggatgc gcgcatagaa ttccggcaca tccagcggca
     1261 acaggatttc atcgaacgca acgccgaacg cgcgcaacac gagccacggg cgcagcgacc
     1321 aggacgaata attcttgctg ccgacgatga gggttggatt tgtggacatg tggattccta
     1381 tacgggctcc ggcagccagc cggcaccgga ttcgggttcg gcgcggtact cggccagttc
     1441 ggcatcgatg ctttccgcga tcgcgcgcgc acgcggccag tcgatcgcgg ccaccatcac
     1501 gttgaccagc ggcatcggcg gcaactcgcc gattgcgccg gtgaggtact ggccaccgac
     1561 gaaggcggga atgccctcgg cctcgagcgc gttcttgacc agattcgcgt cgatgatgtt
     1621 ttcggcccgg tagacgattt gcactttcca gttccctgcc cgtttgcgat cacgctagcg
     1681 catcgccctg cggacgtaaa gccgctagaa ttccgcgttc gctcccgaca aaacgccatg
     1741 accgaaatca gccacaattt catccgccag atcgtccttg acgagcgcgc cgccggcaag
     1801 cacggcggcc gcgtcggcac gcgcttcccg cccgagccga acggttatct gcacctgggc
     1861 cacgccaagt cgatctgcct gaatttcggc atcgcgcgcg agttcggcgg cagctgcaac
     1921 ctgcgcttcg acgacaccaa tcccgcgaaa gaggacgtcg aatacgtcga gtcgatcaag
     1981 gcagacgtgc aatggctcgg cttcgactgg acggaactgc gccacgcttc ggattatttc
     2041 gaggtgttct atcgctgcgc ggaaaaactc atccgcgacg gcaaggcctt cgtctgcgat
     2101 ctttcggcgg acgaagtgcg cgaataccgc ggcacgctga ccgagcccgg tcgcaactca
     2161 cctttccgcg atcgcagcgt cgacgaaaac ctcgacctgt tccggcgcat gcgcaagggc
     2221 gagttcgccg acggcgccaa gaccctgcgt gcgaagatcg aaatggccag cggcaacatc
     2281 aacctgcgcg atccggccct gtaccgcatc cgcaaggtgc agcaccagaa tacgggcgat
     2341 gcctggtgca tctacccgat gtacgactac gcgcattgca tctcggatgc ggttgaaggc
     2401 atcacgcatt cgctgtgcac gctcgagttc gaggatcacc gcccgctcta cgactggtgc
     2461 ctggcgcaga tcgacctgcc gaatcatccc gaactcgtgc agccgctcgt cgatgccggg
     2521 ttcaagacga acgtgagtac gccgcgccag atcgagttcg cgcgcggcaa cctcgactac
     2581 acggtgatga gcaaacgcaa gctcatggaa ctggtgcgcg acggcctcgt cgccggctgg
     2641 gacgatccgc gcatgccgac gctctcgggc ctgcgccggc gcggctttcc acctgcggcg
     2701 atccgcacgt tctgggaacg cgcgggcgtg accaagcaaa attccaccat cgaattttcc
     2761 gtgctcgaag gctgcgtgcg cgaagtgctg gatgcgaacg cgccgcgccg cttcgccgtg
     2821 ctcgatccgc tgaaactcgt catcacgaac ctgccggacg atcacgccga aacgctgacg
     2881 tttccgaatc atcccaagaa cgaaaccttc ggcgcgcgca gcgtcgcgtt cgcgcgcgag
     2941 ttgtggatcg agcgcgagga tttcatggaa acgccggtga agggcttcca tcgcctcgcg
     3001 ccgagcagtg aagtgcgcct gcgcggcgtc ggcatcgtca agtgcacgga tgtgcaaaag
     3061 gatgcca
//
LOCUS       DASRVO010000539         5421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_327816, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000539 DASRVO010000000
VERSION     DASRVO010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327816"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..388
                     /gene="rplQ"
                     /locus_tag="VFF05_06700"
     CDS             5..388
                     /gene="rplQ"
                     /locus_tag="VFF05_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZW51529.1"
                     /translation="MRHMKSGRALSRTSSHREAMFKNMASSLFKHELIKTTLPKAKEL
                     RRVAEPLITLAKADSVANRRLAFARLRDKVAVGKLFVELGPRYRERKGGYLRILKCGM
                     RVGDNAPMAYVELVDRPRVETEAAE"
     gene            510..1025
                     /locus_tag="VFF05_06705"
     CDS             510..1025
                     /locus_tag="VFF05_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HZW51530.1"
                     /translation="MLNPFRWPFRTQCIAGFVVCAALLAYAYFVQFQLGIEPCPLCIF
                     QRLAFIAMGIVFLAGAIHAPGASGRRAYGLLVLVGAAIGVGIAAYHMWVQHLGPDPMA
                     GCTPGWNYMVENYSLKYAWSKTIEHAFTGHADCAEVNWTFLGMAMPFWTLVCYALIGA
                     GAAWAGFRRRD"
     gene            1101..2480
                     /locus_tag="VFF05_06710"
     CDS             1101..2480
                     /locus_tag="VFF05_06710"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HZW51531.1"
                     /translation="MPMQADPRNLSAVREPRDWAPDSWQRKPAVQQPSYPDTDALAAA
                     LVQLRALPPLVTSWEVLALKRKLADAAEGRCFLLQGGDCAESFADCTSPIISNRLKVL
                     LQMSLVLVHGLRLPVVRIGRFAGQYAKPRSADTETRAGETLPSYRGDIVNAPEFTAAA
                     RRPDPHRLIEAHSKSALTMNFTRALIDGGFADLHHPEYWDLAWVEHSPLQDEYRRMVE
                     AIGDSVRFMETLAGQIGSFGKVDFYTSHEALLLHYEQGQTRQVPRQWGWFNLSTHFPW
                     IGMRTAQLDGAHVEYCRGIRNPVGLKVGSGVQPDALLRTLDVLNPDNEPGRITLIHRM
                     GAVKIASELPPLLQAVKTEGRRVLWCCDPMHGNTEALANGVKTRRFANIRAELDAAFD
                     IHAAHGTRLGGVHLELTGENVTECLGGARDLAEADLARAYKSNVDPRLNYEQSLELAM
                     LIVRKRGHR"
     gene            complement(2541..4019)
                     /locus_tag="VFF05_06715"
     CDS             complement(2541..4019)
                     /locus_tag="VFF05_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZW51532.1"
                     /translation="MKSALGLCGGGAGILLALAGSACQAQALSAEVRKYVVHDDPLIA
                     LTHVRVIDGTGAAAQDDRTVVLRNGKIAAIADAAAPLPTGARVVDLSGDSVIPGLVGM
                     HDHLMYTASINIDEDGKIPPPGFLVTELAFSAPRLYLASGVTTMRSTGSIEPYTDLNI
                     RNLIDSGAMPGPHIDVTGPYLDGPGSFFPQMAELTSAVQARQTVDFWVDQGVTSFKAY
                     MDISEPALGAAIAEAHRRHTKVTGHLCSVDYPEAIKLGIDDLEHGPVFTDSEFVPGRK
                     PGVCPQIPAIAASWVDRDIDSAPVRELIRNLVSHRVAVTSTLPVFEASIPTRPLLSRR
                     QMQMLSAESLRSYLVARAGAATATGRAAKSEAAMKKEMAFERAFVAAGGLLLAGPDPT
                     GNGGVLPGFGDQREVELLVEAGFTPLEAIRIATENGARYQGRLDRIGTIGVGKQADLV
                     VIRGDPAAKIDDIENVALVFKDGVAYDPAKLIDSVRGMVGIR"
     gene            complement(4016..4579)
                     /locus_tag="VFF05_06720"
     CDS             complement(4016..4579)
                     /locus_tag="VFF05_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51533.1"
                     /translation="MVSLAQLWLPILLSGVFVFIASSILHMLLKFWHMPDYHGFTNED
                     EVRAAVRKGNPAPGMYMLPWCKMEEMKQPAAQAKFTEGPVGFIILRANGMMNMGKSLG
                     SWFVFCLIVALFSGYIAIHALPVAAPYLHVFQIVGTAAFMGFAFGALPMAIWWGQPWG
                     AVAKDMIDGLIYASITAGTFGCFWPTS"
     gene            complement(4634..>5421)
                     /gene="fusA"
                     /locus_tag="VFF05_06725"
     CDS             complement(4634..>5421)
                     /gene="fusA"
                     /locus_tag="VFF05_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973924.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZW51534.1"
                     /translation="RYEHNKELNESVVRGLSDLHLKLMLERMKERYGVEVKTRPPRIA
                     YRETVAGRAEGHHRHKKQTGGAGQFGEVFLRVEPLPRGAGFEFADEVKGGTIPNQFIP
                     AVEKGVRQVLEHGAIAGYQMQDVRVIVYDGKYHPVDSKEVAFVSAGKKAFLEAISKAK
                     PQVLEPIVNLEVFVPDAHMGDVTGGLAGKRARISGTDSLRGGEIVIKAQVPLAEVTDY
                     QTELKAMTGGQGRYAIEFSHYDAVPAQVQKQLAEAYKPKVEEE"
BASE COUNT          933 a         1764 c         1743 g          981 t
ORIGIN      
        1 acttatgcgt cacatgaaat ccggtcgcgc cttaagccgc acctcgagcc accgtgaggc
       61 catgttcaag aacatggcgt cgtcgctgtt caagcacgaa ttgatcaaga ccacgctgcc
      121 caaagcgaag gaattgcgcc gcgtcgccga gccgctgatc acgctggcca aagccgacag
      181 cgtcgccaac cgccgcctcg ccttcgcgcg ccttcgcgac aaggttgccg tcggcaagct
      241 cttcgtcgaa ctcggcccgc gctaccgcga gcgcaagggc ggctacctgc gcatcctcaa
      301 gtgcggcatg cgcgttggcg acaatgcgcc gatggcctac gtcgaattgg tcgaccgtcc
      361 gcgcgttgaa accgaagccg cggagtaagc cgctgcgcac gttcctgaaa aggcctcggc
      421 gggaaatcgc cggggccttc gtgcttccag cgccggaaca tcggcgcgcg cgcatggtct
      481 gagtcgcatc ttccaggttg ggatccagaa tgctcaatcc attccgctgg ccgttccgca
      541 cgcaatgcat cgccggattc gtcgtctgcg ccgcgctcct cgcctatgcg tatttcgtgc
      601 aattccagct cggcatagag ccgtgtccgc tgtgtatttt ccagcgcctg gccttcatcg
      661 ccatgggcat cgtcttcctc gccggcgcca ttcatgcgcc gggtgcgagc ggccgccgtg
      721 cctatggcct gctcgtgctg gtcggcgcgg ccattggcgt cggcatcgcc gcctatcaca
      781 tgtgggtgca gcacttgggt cccgatccca tggccggctg tacgccgggt tggaactaca
      841 tggtcgaaaa ttattcgctg aagtacgctt ggtcgaagac gatcgagcac gcctttaccg
      901 gccacgccga ctgcgccgag gtcaactgga ccttcctcgg catggccatg ccgttctgga
      961 ccctggtctg ctacgcgctg atcggcgcgg gagcagcgtg ggcgggattc cgccgccgcg
     1021 attgaatcgc tgccattgaa tcggcgcttg catggcgctg ccgctgtggc atgatggaat
     1081 gccgcagtgc aacatgagcg atgccgatgc aagccgatcc gcgcaacctg agcgccgtcc
     1141 gcgagccccg cgactgggcg ccggattcgt ggcaacgcaa gccggcggtg caacagccat
     1201 cgtatccgga caccgacgcg ctggccgccg cacttgttca attgcgcgcg ctgccgccgc
     1261 tggtaacttc gtgggaagtc ctcgctctaa agcgcaagct ggccgacgcc gccgagggcc
     1321 gctgtttcct gttgcagggc ggcgattgcg cggaaagttt tgccgactgc acgtcgccca
     1381 tcatctccaa ccggttgaaa gtgctgttgc aaatgagcct ggtgctggtg cacgggctca
     1441 ggctgccggt ggtacgcatc ggccgtttcg ccggccagta cgccaaaccg cgctcggccg
     1501 acaccgaaac ccgggccggc gaaaccctgc cgagctatcg cggcgacatc gtcaatgcgc
     1561 cggaattcac cgctgccgcg cggcgacccg atccgcatcg tctcatcgaa gcgcattcca
     1621 aatcggcgct gaccatgaat ttcacccgcg ccctcatcga cggtggcttc gccgacctgc
     1681 atcatcccga atactgggat ctggcttggg ttgagcattc gccgttgcag gacgagtatc
     1741 ggcgcatggt cgaggcgatt ggcgattcgg tgcgtttcat ggagaccctg gccggccaga
     1801 tcggcagttt cggcaaggtg gatttctata cttctcacga ggcgctgttg ctgcattacg
     1861 aacaagggca aacgcgccag gtaccgcgcc agtggggctg gttcaacctg tccacgcatt
     1921 tcccgtggat cggcatgcgc accgcgcaac tcgacggtgc gcacgtcgaa tactgccgcg
     1981 gcatccgcaa cccggtcgga ctcaaggtcg gatccggcgt gcagccggat gcgctgctgc
     2041 gcacgctcga tgtgctgaat ccggacaacg agccaggtcg tattacattg attcaccgta
     2101 tgggggcagt gaagatcgcc agcgaacttc cgccgctgct gcaggcggta aagacggaag
     2161 gtcggcgcgt gctgtggtgc tgcgatccca tgcatggcaa caccgaggcg ctggcgaacg
     2221 gcgtcaagac gcgtcgcttc gccaacatcc gcgccgagct cgacgcagcc ttcgacatcc
     2281 atgccgcgca cggcacgcgc ctgggcggcg tgcacctgga actcaccggc gaaaacgtca
     2341 ccgaatgtct gggtggagcg cgcgacctgg ccgaggccga cctcgcgcgg gcctacaaat
     2401 ccaatgtcga tccgcgcctg aattacgaac aatccctcga attggcgatg ctgatcgtgc
     2461 gcaagcgcgg ccaccgctag aaccgcgcgc gccaagcgcg gaaagacgta cggatcggcg
     2521 tactggcggc ctcaagccgc tcagcggatc ccgaccatcc cgcgcaccga atcgatcaac
     2581 ttcgccggat cgtaggcgac gccgtccttg aacacgagcg cgacgttttc gatgtcgtcg
     2641 attttcgccg cgggatcgcc gcggatcacg acgaggtcgg cttgcttgcc gaccccgatc
     2701 gtgccgatgc ggtcgagccg gccctggtaa cgcgcgccgt tttccgtggc gatgcggatc
     2761 gcttcaagcg gggtgaagcc ggcttcgacc agcaactcga cttcgcgctg atcgccgaaa
     2821 ccgggcagca cgccgccgtt gccggtcggg tcgggcccgg ccagcagtag gccacccgcg
     2881 gcgacgaagg cgcgctcgaa cgccatttcc tttttcatcg ccgcttcgga tttcgcggcg
     2941 cgtcccgtgg cggtcgcggc gccggcgcgc gcgacaagat agctgcgcag cgattcggcc
     3001 gacagcattt gcatctggcg ccgcgacagc agcggccggg tgggaatcga ggcttcgaac
     3061 acgggtagcg tggaggtcac cgccacgcga tgggaaacga ggttgcggat cagctcgcgt
     3121 accggcgcgc tgtcgatgtc gcgatcgacc cacgaggccg cgatcgcagg aatctgcgga
     3181 cacacgccgg gcttgcggcc gggcacgaat tcagaatcgg tgaagaccgg gccgtgttcc
     3241 aggtcgtcga tgccgagctt gatcgcttcc ggatagtcga ccgagcacag gtgtccggtg
     3301 accttggtgt gccgccgatg cgcctcggcg atggcggcgc ccagggcagg ctcggaaatg
     3361 tccatgtagg ccttgaacga agtcacgcct tggtcgaccc agaaatccac ggtctggcgc
     3421 gcctgcacgg cactcgtcag ttcggccatc tgcgggaaga agctgccggg accatccagg
     3481 tagggaccgg tcacgtcgat gtgcgggccg ggcatggcgc cgctgtcgat gagattgcgg
     3541 atgttcaggt cggtgtatgg ctcgatgctg ccggtgctgc gcatcgtcgt cacgccggaa
     3601 gcaaggtaca gccgcggcgc tgagaacgcg agttcggtga cgagaaaccc cggcggcgga
     3661 atcttgccgt cctcgtcgat attgatcgaa gcggtgtaca tcaggtgatc gtgcatgccg
     3721 acgaggccgg ggatcacgct gtcgccggaa agatcgacga cgcgcgcacc cgtgggcagc
     3781 ggcgccgcgg cgtcggcgat cgcggcgatc ttgccgttgc gcagcactac ggtgcggtcg
     3841 tcctgcgcgg cggcgccggt gccgtcgatc acgcgcacat gggtgagcgc gatcagcgga
     3901 tcgtcgtgga cgacgtattt ccgcacttcg gcggaaagcg cctgcgcctg gcacgcgctg
     3961 ccggcgagcg ccagcaggat gccggcgccg cccccgcaca aacccaatgc cgacttcacg
     4021 atgtgggcca gaaacagccg aacgtgccgg ccgtgatcga tgcgtagatc aagccgtcga
     4081 tcatgtcctt ggccaccgcg ccccagggct ggccccacca gatggccatc ggcaacgcgc
     4141 cgaacgcgaa gcccatgaag gctgcggtgc cgacgatctg gaagacgtgc aggtacggcg
     4201 cggcgaccgg cagcgcgtgg atcgcgatgt acccggaaaa cagcgcgacg atcaggcaga
     4261 acacgaacca cgatccgagc gacttgccca tgttcatcat gccgttggcg cgcaaaatga
     4321 tgaaaccgac tggtccttcc gtgaacttgg cctgcgcagc gggctgtttc atctcctcca
     4381 tcttgcacca cggcagcatg tacatgcccg gcgccggatt gcccttgcgc acggcggcgc
     4441 ggacttcgtc ttcgttggtg aagccgtggt agtcaggcat gtgccagaac ttgagcagca
     4501 tgtgcaggat actgctggcg atgaacacga acacgccgga cagcagtatg ggcaaccaca
     4561 attgtgcgag cgacaccatg acatttcctc ccattcgatt ggttgatgca acacgactaa
     4621 aaatccacag acatcattct tcttccacct tcggcttgta ggcctccgcc aactgcttct
     4681 gcacctgcgc tggcaccgca tcgtagtggc tgaactcgat cgcgtagcgg ccctggccgc
     4741 cggtcatggc cttgagttcg gtctggtagt cggtgacctc cgccagcggc acctgtgcct
     4801 tgatcacgat ctcgccgccg cgcagcgaat cggtgccgct gatgcgcgcg cgcttgccgg
     4861 cgagtccgcc ggtcacgtcg cccatgtgcg cgtcgggcac gaacacttcg agattgacga
     4921 tgggctcgag cacctgcggc ttggccttgc tgattgcctc gaggaatgcc ttcttgccgg
     4981 cgctgacgaa ggcaacttcc ttggaatcga ccggatggta cttgccgtcg tagacgatga
     5041 cgcgcacgtc ctgcatctgg tagccggcaa tggcgccgtg ttccagtacc tggcgcacgc
     5101 ccttttccac cgcggggatg aactggttcg ggatcgtgcc gcccttgacc tcgtcggcga
     5161 attcgaagcc cgcgccgcgc ggcagcggtt cgacgcgcag gaacacttcg ccgaactggc
     5221 cggcgccgcc ggtctgcttc ttgtggcgat gatggccctc ggcgcggccg gcgacggtct
     5281 cgcggtaggc gatgcgcggc ggccgcgtct tgacctcgac gccatagcgt tccttcatgc
     5341 gctcgagcat gagcttcagg tgcaggtccg acaggccgcg caccaccgat tcgttgagtt
     5401 ccttgttgtg ctcgtagcgg a
//
LOCUS       DASRVO010000540         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_197897, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000540 DASRVO010000000
VERSION     DASRVO010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197897"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFF05_06730"
     CDS             complement(<1..1035)
                     /locus_tag="VFF05_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZW51535.1"
                     /translation="MIWLIVGALVVLAALGAPLFAIIAAIALFGFHAAGEPGAAVAIE
                     FYRLADMPALIAIPLFTLAGYLLAESQAPRRLVRLTNALFGWLPGGLAIVSVCACTLF
                     TAFTGATGVTLIALGAVLYPALRQAHYEERFSLGLLTAAGSLGLLLVPSMPLILYGVV
                     AQQFHTTPPVSIDALFKAGLLPCLLMVVMLSIYSAWKARDLPRPPASSGREVWAALKD
                     AAWELPLPFVILIGIYTGKLAASEAAAATALYVLIVTVLIRREIKIAELPRVIREAML
                     LVGAILIVLGFSLALTNWLIDGDVPEKLFSALQAGISGKFAFLLLLNAFLLVFGMLLE
                     GFPAIIILVPL"
     gene            complement(1032..1538)
                     /locus_tag="VFF05_06735"
     CDS             complement(1032..1538)
                     /locus_tag="VFF05_06735"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZW51536.1"
                     /translation="MQRALEILRRIEDWLLAILVLLLVVLAGGQIVLRDFFHTGISWA
                     DPLMRQLVLWTGMLGALAAVRDEKHIALDVLQRFLSPLAQKIARILTFGFAAAICAAL
                     AWYSYVMLDVDYASATPSSAFAALPAWIPETILPTAFGLMALRFVLRAFLPPAHVPPL
                     LHPIEPSQ"
     gene            complement(1541..>2409)
                     /gene="dctP"
                     /locus_tag="VFF05_06740"
     CDS             complement(1541..>2409)
                     /gene="dctP"
                     /locus_tag="VFF05_06740"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HZW51537.1"
                     /translation="GAEAVKKDTDGRVEIKYYPGGVMGDEPTVLRKIRIGQLQGGAFT
                     GGELSQIVKDAQIYSLPFLFKNLDEVDKVRAQLDPLLKQEFDKAGFDVAGISGGGFAY
                     LMSVNDIKSKDDLKAAKVWVPQGDRVAEAGFKAGGVTPISLPLADVYTSLQTGLIDTV
                     ANTPAGAIAFQWHTKVRHMVDLPITYVVGMLVVDKKVFGTLSPADQKAVNDDLAAAFA
                     RLEKINRDDNTQAKLALQKQGVEIFTPNAAETASWDAVGAQARKQLQAAGEVTPEMAA
                     ALDKALASARSK"
BASE COUNT          462 a          784 c          778 g          385 t
ORIGIN      
        1 cagcggcacc aggatgatga tcgccgggaa tccttcgagc agcatgccga acacgagcag
       61 gaacgcgttg agcagcagca ggaaggcgaa cttgccggaa atccccgcct gcagggccga
      121 gaacaatttt tccggcacgt cgccgtcgat caaccagttc gtcagcgcca gcgaaaatcc
      181 gagcacgatc aggatggcac cgaccagcag catggcctcg cggatcacgc gcggcaattc
      241 cgcaatcttg atttcacggc ggatcagcac tgtcacgatc agcacgtaca aggccgtcgc
      301 cgcggcggct tcggatgcgg ccagcttgcc ggtgtagatg ccgatcagga tcacaaaggg
      361 caagggcagt tcccaggccg catccttgag tgccgcccag acttcgcgcc cgctggacgc
      421 cggcgggcgt ggcagatcgc gcgccttcca cgcgctgtag atcgacagca tcacgaccat
      481 gagcaggcat ggcagcaggc ctgccttgaa cagcgcatcg atgctgaccg gcggcgtggt
      541 gtggaattgc tgcgcgacca cgccgtacag gatcagcggc atcgacggca ccagcaaaag
      601 gccgagactg ccggcggcgg tgagcaggcc cagcgaaaag cgttcctcgt aatgcgcctg
      661 gcgcagcgcc ggatacagca ccgcgccgag tgcgatcaag gtgacgccgg tcgcaccggt
      721 gaatgcggtg aacagcgtgc aggcgcacac ggagacgatc gccaatccac ccggcagcca
      781 gccgaacagc gcattggtca ggcgcaccag tcgccgtggc gcctggcttt ccgccagcag
      841 gtatcccgcc agcgtgaaca gcggaatcgc gatcagcgcc ggcatgtcgg cgaggcggta
      901 gaactcgatc gcaaccgccg cgccgggttc gccggcggcg tggaaaccga acagcgcgat
      961 cgccgcgatg atcgcgaaca gcggcgcgcc aagcgccgcc agcaccacga gcgcgccaac
     1021 gatcagccag atcattgcga cggctcgatg ggatgcagca acggcggcac atgcgccggt
     1081 ggcaggaagg cgcgcagcac gaagcgcagc gccatcagcc cgaatgccgt gggcaggatc
     1141 gtctcgggaa tccaggccgg cagcgccgca aacgcactcg acggcgtggc gctggcgtaa
     1201 tccacgtcca gcatcacgta gctgtaccag gccagcgccg cgcagatggc agcggcgaat
     1261 ccgaacgtga ggatgcgcgc gatcttttgc gcaagcgggg aaagaaagcg ttgcagaacg
     1321 tccagcgcga tgtgcttctc gtcgcgcacg gcggcgagcg cgccaagcat gcccgtccac
     1381 agtaccagct ggcgcatcag cggatcggcc caggaaattc cggtgtggaa aaaatcgcgc
     1441 aggacgatct ggccgcccgc aagaacgacg agcagcagaa cgagaatggc tagcagccag
     1501 tcctcgatgc ggcgcaggat ttccagcgcg cgctgcaagc ttacttgctc cgcgccgaag
     1561 ccagcgcctt gtccagcgcc gccgccattt ccggcgtgac ttcgccggca gcctgcagct
     1621 gtttgcgcgc ttgcgcaccg accgcgtccc acgatgcggt ttcggccgca ttcggcgtga
     1681 agatttccac accctgcttc tgcaacgcaa gtttggcttg cgtgttgtcg tcgcggttga
     1741 tcttctccag gcgtgcgaaa gcggcggcga gatcgtcgtt caccgctttc tgatcggccg
     1801 gtgacagcgt tccgaacact ttcttgtcga ccacgagcat gccgacgacg taggtgatcg
     1861 gcaggtcgac catgtgccga actttcgtgt gccattgaaa cgcgatcgca ccggccgggg
     1921 tgttggcgac ggtgtcgatc aatccggttt gcaggctggt gtagacgtcg gccagcggca
     1981 acgaaatcgg cgtgacgcca ccggccttga agccggcttc ggcgacgcgg tcgccctgcg
     2041 gcacccagac tttcgcggct ttcagatcgt ccttgctctt gatgtcgttc acgctcatca
     2101 ggtaggcgaa gccgccgccg ctgatgccgg caacgtcgaa cccggccttg tcgaattcct
     2161 gcttgagcag cggatcgagc tgcgcgcgca ccttgtcgac ttcgtccagg ttcttgaaca
     2221 ggaacggcag gctgtagatc tgcgcgtcct tgacgatctg cgacagctcg ccgcccgtaa
     2281 aggcgccgcc ctgcaactgg ccgatgcgga tcttgcgcaa cacggtgggt tcgtcgccca
     2341 tcacgccgcc ggggtaatac ttgatctcga cgcggccgtc ggtgtccttc ttcaccgcct
     2401 cggcgccgg
//
LOCUS       DASRVO010000541         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_378784, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000541 DASRVO010000000
VERSION     DASRVO010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378784"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFF05_06745"
     CDS             complement(<1..255)
                     /locus_tag="VFF05_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51538.1"
                     /translation="MRQIFSSPRIENVERIVTLLNEAGIETRVTNRRAYAGHDYKGPS
                     YSARQDSSTWPAVWIVKAEDQPRARAMLRDIGIAPATRFAE"
     gene            complement(252..1130)
                     /gene="tesB"
                     /locus_tag="VFF05_06750"
     CDS             complement(252..1130)
                     /gene="tesB"
                     /locus_tag="VFF05_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HZW51539.1"
                     /translation="MSNPIVGELIDLLQLERLEQNLFRGQSRDIGTKYVFGGQVLGQA
                     LSAAQRTVDPARAVHSLHAYFLRAGDIEAPIVFEVDRARDGRSFSVRRIVAIQHGEVI
                     LNAAASFQIAEAGVEHQLSMPDVPPPEDIEPPAPIPSEELAKLPIKAQRWLARKGPFE
                     FRPVYPRNELKPAKRPPFQQIWFRLAGRAPDDDTLHRAMLAYASDFHLIGTATLPHGI
                     SYLQHNVQMASLDHAIWFHRPFRMDDWLLYACDSPSAQGARGLTRGMIYTRDGKLVAS
                     TAQEGMLRVLPDTPTP"
     gene            complement(1139..1705)
                     /locus_tag="VFF05_06755"
     CDS             complement(1139..1705)
                     /locus_tag="VFF05_06755"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZW51540.1"
                     /translation="MAIAPPIHDQPLPYVTRLEQRPLSAIDLVVIHCTELPDMTMARE
                     FGERIRYEKSQTGNSGHYYIDTDGTIYRYVPDERVANHTYGYNPRSIGIELSNVGRYP
                     HWGDSQHQKWTTPYTRAQIDALLALLRQLRNELPNLKYIAGHEDLDTRLEPASDDPSI
                     MLRRRQDPGPLFPWQDIVPASGLQRLQP"
     gene            1720..>2014
                     /locus_tag="VFF05_06760"
     CDS             1720..>2014
                     /locus_tag="VFF05_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZW51541.1"
                     /translation="MKPFLILSHGLHSSPDATKVSALAAIAEPLGFSSARPDYRDLDA
                     SGDVRRIDERIARLVEHAPQNAPVILAGSSMGAFVSGFASLQLNCVGLFLMALP"
BASE COUNT          328 a          605 c          691 g          390 t
ORIGIN      
        1 ctcggcaaag cgcgtggccg gcgcgatgcc gatgtcgcgc agcatcgcgc gcgcgcgcgg
       61 ctggtcctcg gccttgacga tccataccgc cggccatgtc gatgaatcct gccgggcgct
      121 gtagctcggg cccttgtaat cgtggccggc gtaggcgcgg cggttggtca cgcgcgtctc
      181 gatgccggct tcgttgagca gggtaacgat gcgctcaacg ttctcgatgc gcggcgagga
      241 aaagatctgt ctcatggtgt gggcgtgtcc ggcagcacgc gcagcatgcc ttcctgcgcg
      301 gtcgaggcga cgagcttgcc gtcgcgcgtg tagatcatgc cgcgggtcag gccgcgcgcg
      361 ccctgcgcgc tcgggctgtc gcaggcatac agcagccagt cgtccatgcg aaacgggcgg
      421 tgaaaccaga tcgcgtgatc caggctcgcc atctgcacgt tgtgctgcag ataggaaatc
      481 ccgtgcggca gcgtggccgt gccgatcagg tgaaaatccg aagcgtaggc gagcatcgcg
      541 cgatgcaggg tatcgtcgtc cggcgcgcgt ccggcgaggc ggaaccagat ctgctggaac
      601 ggcggtcgct tcgccggctt caattcgttg cgcggataga ccggacggaa ttcgaatggc
      661 cccttgcgcg cgagccagcg ctgcgccttg atcggcagtt tggcgagttc ttcggacgga
      721 atcggagcgg gtggttcgat gtcttccggc ggcggcacat ccggcatgct gagctgatgc
      781 tcgacgccgg cttcggcgat ctggaacgag gcggcggcgt tgaggatgac ctcgccatgc
      841 tggatcgcga cgatgcggcg cacggaaaaa ctgcggccat cgcgggcgcg gtccacttcg
      901 aacacgatcg gtgcctcgat gtcgccggcg cgcaggaaat acgcgtgcag cgaatgcacc
      961 gcacgcgccg gatccaccgt gcgctgcgcc gccgacaagg cttgcccgag cacctgcccg
     1021 ccgaacacgt acttggtgcc gatgtcgcga ctctggccgc gaaacaggtt ttgctcaagc
     1081 cgttcgagct gcaacaggtc gatgagttcg ccgacgatgg gattcgacat ggaaattcct
     1141 aaggctgcaa acgctggagt ccgctcgcag gaacaatatc ctgccacgga aacaacggtc
     1201 ccggatcctg ccgccggcgc agcatgatgc tcggatcgtc gcttgccggt tccaggcgcg
     1261 tgtcgaggtc ttcgtgtccg gcgatgtatt tcaggttcgg cagttcgttg cgcaactgcc
     1321 gcagcagggc gagcagggcg tcgatctgcg cgcgggtgta gggcgtcgtc cacttttgat
     1381 gttgactatc gccccagtgc ggatagcgac cgacattcga cagttcgatg ccgatcgaac
     1441 gcgggttgta gccgtaggtg tggttggcca cgcgctcgtc gggtacgtag cggtagatcg
     1501 tgccgtcggt gtcgatgtag taatgcccgc tgttgccggt ctgcgatttt tcgtagcgga
     1561 tgcgctcgcc gaattcgcgc gccatcgtca tgtccggcaa ttcggtgcaa tggatcacga
     1621 cgaggtcaat cgccgacagc gggcgttgtt ccagccgcgt gacgtagggc aggggctggt
     1681 cgtggatggg cggtgcgatg gccatgcgat aattgtcgca tgaagccttt ccttatcctc
     1741 tcccatggcc tgcacagcag tccggacgcc acgaaagtga gcgcactggc tgctatagcc
     1801 gagccgctgg gattttccag cgcgcgcccg gattaccgcg atctcgacgc cagcggcgac
     1861 gtgcgccgca tcgacgagcg cattgcgcgg ctggtcgaac acgcgccgca aaatgcgccg
     1921 gtgatcctgg ccggatcgag catgggcgcg ttcgtttcgg gattcgcgtc gctgcaattg
     1981 aattgcgtcg gcttgtttct catggccttg ccga
//
LOCUS       DASRVO010000542         1061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_119744, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000542 DASRVO010000000
VERSION     DASRVO010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119744"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VFF05_06765"
     CDS             complement(<1..876)
                     /locus_tag="VFF05_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51542.1"
                     /translation="MSVAATAARSNANMPRSAVVEGNEAAAIGVGLARPDVVAVYPIT
                     PQSTLVEHLSQMIADGRLKADLMNVEGEHAVLNGLQGVALAGGRTFTATCGPGIAFMF
                     EPYVRTPGLRLPMVATLVTRDLLSPQCVWGGQQDAMVVREAGWIQMYCETVQEILDTT
                     VMAYKIAEHHNVMLPVNVCHDGNYLSFGVTRVELPEQDEVDKFLGPPAINWHIALDPE
                     RPMGVDPLTGGASGPGARNFVRYRKGECRGMQNALRVISEVHAEWSERFGRSHAPLVE
                     EYRLDDADYALVTIGS"
     gene            complement(873..>1061)
                     /locus_tag="VFF05_06770"
     CDS             complement(873..>1061)
                     /locus_tag="VFF05_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZW51543.1"
                     /translation="LRPVIDRRKCVQCGLCWLYCPVQCVTPKPRWFEFDLKVCKGCGV
                     CAQECPQRAIRMIEEAGR"
BASE COUNT          174 a          344 c          356 g          187 t
ORIGIN      
        1 gctgccgatg gtgacaaggg catagtcggc atcgtcgagc cgatattctt cgacgagcgg
       61 cgcgtgactg cggccgaatc gctcgctcca ctccgcatgc acttcgctaa tgacgcgcag
      121 cgcgttctgc atgccgcggc attcgccttt gcggtagcgc acgaaattgc gcgcgcccgg
      181 cccgctggcg ccgccggtca gcgggtcgac gcccatcggc cgttcgggat cgagcgcgat
      241 atgccaattg atcgccggcg ggccgaggaa tttgtcgacc tcgtcctgct ccggcagttc
      301 gacgcgggtg acgccgaagg acaggtaatt gccgtcgtgg cagacgttca ccggcagcat
      361 gacgttatgg tgttcggcga tcttgtaggc catgacggtg gtgtcgagaa tctcctgcac
      421 tgtttcgcaa tacatctgaa tccaaccagc ttcgcgcacg accatggcgt cctgctgccc
      481 gccccagacg cattgcgggc tgagcagatc gcgcgtaacc agcgtggcga ccatcggcaa
      541 gcgtaggccg ggcgtgcgca catagggctc gaacatgaag gcgatgcccg gtccgcaggt
      601 ggcggtgaag gtgcgcccgc cggccagcgc cacgccctgc aggccgttga gcacggcgtg
      661 ctcgccttcg acgttcatca ggtcggcctt gaggcggccg tcggcgatca tctgcgagag
      721 atgctcgacc agcgtggatt gcggtgtgat cggatagacc gcgaccacgt cgggccgcgc
      781 cagaccgacg ccgatcgccg ccgcttcgtt gccttcgact acggcggaac gcggcatgtt
      841 ggcgttggaa cgcgcggcgg tggcggcaac actcatcggc cggcctcctc gatcatgcgg
      901 atggcgcgct gcgggcattc ctgcgcgcag acgccgcagc ccttgcacac tttgaggtcg
      961 aactcgaacc agcgcggttt gggggtgacg cactgcaccg ggcaatagag ccagcacagc
     1021 ccgcattgca cgcatttgcg ccggtcgatc accggtcgca g
//
LOCUS       DASRVO010000543         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_119756, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000543 DASRVO010000000
VERSION     DASRVO010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119756"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VFF05_06775"
     CDS             <1..1099
                     /locus_tag="VFF05_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51544.1"
                     /translation="GAVLGQQVDVSGKDFLSGAGDAKLASIAQQAAAAGKSLEVTAPD
                     YWQSKVAAKLHASAADVAIKSNNGFFENVLVKVVDAAPAKPVAASPDAQAVKATAEKV
                     AAQKAAEEKAAQEKAVAEQAAAQRAAAQAAAAKAAADKAAAEKAAAEKAAADKAAAEK
                     AAAEKAAAEKAAAEKAIADKRATARNTMLKNLNEGRPAEGDLQVGQLQTGDVVYVDGD
                     LRGVVRRGSSRLQFYWLQGDLNLERIELSPNGEGRYKVLRAIDASAAPVLRTRSGGTF
                     AAAIPASGSAARKSLQDQYADGRDIADTLKPADLRQGDTIYTGQGAALVVRRVGGQLA
                     RYWLDGPLDLGQIGLKNQGGNAYKVLSDTVR"
     gene            complement(1138..>1563)
                     /locus_tag="VFF05_06780"
     CDS             complement(1138..>1563)
                     /locus_tag="VFF05_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51545.1"
                     /translation="SVMDDSHIAVHAPGLADAVVEASGTLSIAGTPVAVNAGQAQIAA
                     RYFASALALRNDAIKTGAAGASTAATAIASVAVGLASGNPDSIDAKVNASASKVEAAA
                     NRVCADLQALTQAQNDLAAALPQFKPYATIAAHEANDCE"
BASE COUNT          283 a          485 c          550 g          245 t
ORIGIN      
        1 cggcgcggtg ctgggccagc aggtcgacgt cagcggcaag gactttctgt ccggcgcggg
       61 tgacgccaag ctcgcgagca tcgcgcagca ggctgcggca gccggcaagt cgctggaagt
      121 cacggcgccg gattactggc agtccaaggt cgcggccaag ctgcatgcca gtgcggcgga
      181 cgtggcgatc aagtcgaaca acggtttctt cgagaacgtg ttggtgaagg tcgtcgatgc
      241 cgcgccggcc aagccggttg ctgcatcgcc cgacgcacag gcggtcaagg ccacagcaga
      301 gaaggtcgct gcgcagaagg ctgccgaaga aaaggcggcg caggaaaaag ccgtcgccga
      361 gcaggccgcc gcgcaacgcg ccgccgcaca ggccgcagcc gcgaaagccg cggcggacaa
      421 ggcggccgcc gagaaggccg ctgccgagaa agctgcggcg gacaaggccg ctgcggaaaa
      481 ggccgctgcc gagaaagcgg ctgcggaaaa agccgccgcg gagaaggcca tcgcggacaa
      541 gcgcgcgacg gcgcgcaaca ccatgctgaa gaatctcaac gaaggtcgcc ctgccgaagg
      601 cgatttgcag gtcggccaat tgcagaccgg cgatgtggtc tacgtcgacg gcgatctgcg
      661 cggcgtcgtg cgccgcggca gttcgcgcct gcagttctat tggttgcagg gtgatctgaa
      721 tctcgaacgc atcgagttgt cgccgaacgg cgagggtcgc tacaaggtct tgcgtgcgat
      781 cgacgcgagc gccgcaccgg tgttgcgcac gcgcagtggc ggtacgtttg ccgccgcgat
      841 tccggcgagc ggctcggcgg cgcgcaagtc gctgcaggat caatacgcgg atggccgcga
      901 cattgccgac acgctcaagc ctgcggatct gcgccagggc gacacgatct acaccgggca
      961 aggtgccgcg ctggtggtaa ggcgcgtcgg cggtcagctt gcgcgctact ggctcgatgg
     1021 tccgctggat ctgggccaga tcggactgaa gaatcagggc ggcaatgcgt acaaggtgtt
     1081 gtccgacacc gtcagataac aacgcatgcg caggccgccg caatggcggc ctgctcatta
     1141 ttcacagtcg ttcgcttcgt gtgcggcaat cgtcgcgtag ggtttgaatt gcggtaatgc
     1201 cgcggcgaga tcgttttgcg cttgcgtcag cgcctgcaag tccgcacaga cgcggttcgc
     1261 ggcggcttcg actttggacg cactcgcatt caccttcgca tcgatcgagt ccgggttgcc
     1321 cgaagccagc cccaccgcga cgctggcaat ggcggtcgcc gcggtggacg ctcccgcagc
     1381 gcccgtcttg atggcatcgt tgcgcagcgc cagggcgctg gcgaaataac gcgcggcaat
     1441 ttgcgcctgc cccgcattga cggctaccgg cgtgccggcg atgctcaatg tgccggatgc
     1501 ctcgacgacc gcgtctgcga ggcccggtgc atgtacggcg atatggctgt cgtccattac
     1561 gct
//
LOCUS       DASRVO010000544         1390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_68008, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000544 DASRVO010000000
VERSION     DASRVO010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68008"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1390)
                     /locus_tag="VFF05_06785"
     CDS             complement(<1..>1390)
                     /locus_tag="VFF05_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZW51546.1"
                     /translation="AHDTERPILPVAELYLPPDRLREQLNRHLRAEFVAPGASEHAVG
                     LGAFPAPMLPISQRGAEPAAGLKRFLDSYNGRVLIAVDSAGRREALLEQLAQADLHPQ
                     VVQSWQEFVKGTIKLETAVAAFDDGFALADPPITVLTERQLLGERAQQTRRRKVAARD
                     PETVLRDLSELAIGAPIVHADHGVGRYQGLTKLDVGGGGEFLVVEYANADKLYVPVAQ
                     LHLVSRYSGTAPELAPLHSLGGNAWERAKRKAAQKVRDAAAELLALYAQREARQGRAI
                     AYDRALYAQFAAAFPFEETPDQLQAIEAVIADLASGKAMDRVVCGDVGFGKTEVALRA
                     AFVAATAGKQVAVLVPTTLLAQQHYQNFRDRFADWPVRVEVISRFKSKKETDEALAKL
                     RDGKIDVMIGTHRLLQADVKFKDLGLVVVDEEHRFGVRHKEALKALRAEVDVLTLTAT
                     PIPRTLAMSLEGL"
BASE COUNT          225 a          455 c          468 g          242 t
ORIGIN      
        1 tgagtccctc gagcgacatc gcgagcgtcc tcgggatggg cgtggccgtc agcgtaagca
       61 cgtcgacttc ggcgcgcagc gccttcagcg cttccttgtg gcgcacgccg aagcggtgtt
      121 cctcgtcgac gacgacgagg ccgagatcct tgaatttcac gtccgcctgc agcaagcgat
      181 gcgtgccgat catcacgtcg atcttgccgt cgcgcagttt ggccagcgcc tcgtcggttt
      241 cctttttcga cttgaagcgc gagatcactt ccacgcgcac cggccagtcg gcgaagcggt
      301 cgcggaaatt ctggtaatgc tgctgggcga gcaacgtcgt cggcaccagc acggcaacct
      361 gcttgcccgc ggtcgcggcg acgaaggccg cacgcagggc gacttcggtc ttgccgaagc
      421 cgacatcgcc gcagaccacg cgatccatcg ccttgcccga ggcgaggtcg gcgatcacgg
      481 cctcgatcgc ctgcaactgg tctggagtct cctcgaacgg gaacgcggcg gcgaattgcg
      541 cgtacagcgc gcggtcgtag gcaatggcgc gaccctggcg tgcttcgcgc tgcgcgtaca
      601 gggcgagcag ttcggctgcc gcatcgcgga ctttctgcgc ggccttgcgt ttggcgcgtt
      661 cccaggcgtt gccgccgagc gaatgcaaag gcgccagttc cggcgcggtg ccggaatagc
      721 gcgagacgag atgcaattgc gcgaccggca cgtagagctt gtcggcgttg gcgtattcaa
      781 cgacgaggaa ttccccgccg ccaccgacgt ccagcttggt cagaccctga tagcggccga
      841 cgccgtggtc cgcgtgcacg atgggcgcgc cgatggccag ctccgacaaa tcgcgcaaca
      901 cggtttccgg gtcgcgcgct gcgactttgc gccgacgcgt ctgctgcgcg cgctcgccga
      961 gcaactggcg ctcggtgagg acggtgatcg gcggatcggc gagggcgaaa ccgtcgtcga
     1021 atgcggcgac ggcggtctcc agcttgatcg tgcctttgac gaattcctgc cacgattgca
     1081 cgacctgcgg atgcaaatcc gcttgagcaa gttgctcgag caacgcttcg cggcgaccgg
     1141 ccgaatccac cgcgatcagc acgcgtccgt tgtagctgtc gagaaagcgc ttgagtccgg
     1201 cagccggttc agcgccacgc tgactgattg gcaacatcgg tgccggaaac gcgccgaggc
     1261 ccacggcatg ttcgctcgcc ccgggtgcga caaactccgc gcgcaggtgc cggttcagct
     1321 gctcgcgcaa ccggtccggc ggcagataca gttcggcaac gggcaggatc ggccgttcgg
     1381 tgtcgtgggc
//
LOCUS       DASRVO010000545         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_42933, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000545 DASRVO010000000
VERSION     DASRVO010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42933"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            565..714
                     /locus_tag="VFF05_06790"
     CDS             565..714
                     /locus_tag="VFF05_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51547.1"
                     /translation="MSDNGVTITQTDPQQALRSLNRSDAKTAAALRVCNAILGRPSAS
                     PTPTG"
     gene            complement(734..>1174)
                     /locus_tag="VFF05_06795"
     CDS             complement(734..>1174)
                     /locus_tag="VFF05_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51548.1"
                     /translation="PISFASMVLGNPSRITAVSDQAFTGVDAQTGILLQYQGGQHAVL
                     FTSLETRTPNWACVNGTDARIEIEGVFYTPSRFRLITRDGDVEHFDFPHEGHGLRHQA
                     ATVGRCLRAGLTESDALPLDETIAIMETMDEVRRQIGLTYPSES"
BASE COUNT          189 a          390 c          403 g          192 t
ORIGIN      
        1 cggctgggaa agcgctgcga ggttgccgcg aatccggtcg tgcggcttgt tcggcgtccg
       61 cgcgcgccgc acgcttgcga cgtgaaaatc gccctcttct ttggacgacg cggcctcgct
      121 gccgctgcgg gcacgttgtc cgctggtgtc gccacggcgc ttgtcatcgc cggctgcagc
      181 gccggctcgg ccaagaacgc aggcagcaca agcggttccg tggcttcgtc atcaagcgcg
      241 tcagcgacgt cgtccaaccg cgccgccgac tttcaggcct acacgcaatg cctttcacag
      301 cacggcgtgg cggttccgac gttcagcgcg cggccggact tcagcgcgcg accgcggccg
      361 tcggggtcgt tcgttcggcc cagcggcgca ccaagaagcg gaggccccgg cggtcgattt
      421 ggctttctcg gcagcgccga cccgagcact cgcgcggcga tgcaggcgtg tcaaagcctc
      481 gcgccacaag gcggattcgg cggcggtttc ggacgcggcg gggcgaccat cagcgccgcg
      541 acattcgccg cgtttaagag ctgcatgagc gacaacggcg tgacgataac gcagaccgac
      601 ccgcaacaag cgctgcgttc gctgaatcgc tccgacgcga agaccgctgc cgcgctgagg
      661 gtctgcaacg ccatcctcgg ccgacccagc gccagcccga cgccaacagg gtgacgccaa
      721 cagcgcgacg gagctatgac tcgctggggt aggtcaggcc aatctgccgg cgaacctcgt
      781 ccatcgtctc catgatggcg atggtctcgt cgagcggcag cgcgtcgctt tcggtgagac
      841 cggcgcgcag gcaccgcccg acggtggcgg cttgatgacg caacccgtga ccctcgtgcg
      901 ggaagtcgaa atgctcgacg tcgccgtcgc gggtgatgag ccggaaacgt gacggcgtgt
      961 agaagacgcc ttcgatttcg atgcgcgcat cggtgccgtt gacgcaagcc cagttggggg
     1021 tgcgcgtctc gaggctcgtg aacaacaccg cgtgctggcc gccctggtat tgcaggagga
     1081 tcccggtttg cgcgtcgacg ccggtgaatg cttggtcact gaccgcggtg attctcgacg
     1141 ggttgcccag caccatcgag gcgaacgaga tcgg
//
LOCUS       DASRVO010000546         1769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_93187, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000546 DASRVO010000000
VERSION     DASRVO010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93187"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFF05_06800"
     CDS             complement(<1..1230)
                     /locus_tag="VFF05_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZW51549.1"
                     /translation="MLIPEMKTGKRSGRIIRRLLGALIILAAGLALVAWLLLRASLPQ
                     LDGTRALGGVHAPTTIARDALGVVTIKAQSRNDAMFALGFVHAQERFFEMDLMRRMAA
                     GELAELVGGAAVDLDKQHRPFRMRERALAALATATPDRRATLDAYRDGVNAGLAALTS
                     RPWEYWLLGEKPAPWSDADCILAIDAMFFDLNGADNARELAFAQIKSALGDKAYRFLS
                     ASGGPWDAPLLGPPMNYPELPGASDLDLRKTDPKFLHMPPAHAEERAKPGSNGFAVNG
                     ALTATHAALIADDMHLPLRVPNIWFRTRIEYPNPRRSGQTVDLIGVTLPGMPALVAGS
                     NRHVAWGFTNSYGDWMDFVRVVLDPNDPTRYKTAAGWKVLSQSTQIIKVHNAPDLKLD
                     VRDTQWGPILAKDTDGTP"
     gene            1523..>1769
                     /locus_tag="VFF05_06805"
     CDS             1523..>1769
                     /locus_tag="VFF05_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51550.1"
                     /translation="MLIMSWTLTVSAAVCYVNGTASGANTGASWTDAYTDLQSALNNA
                     SCTEVWVAQGIYKPAASNTAVSFNIGPNVAVYGGFSGS"
BASE COUNT          284 a          536 c          605 g          344 t
ORIGIN      
        1 cggcgtgccg tcggtgtcct tggccaggat cggtccccac tgcgtgtcgc gcacgtcgag
       61 cttcaggtcc ggggcattgt ggactttgat tatttgtgta ctttgcgaca acactttcca
      121 gccagcggcc gtcttgtagc gtgtcgggtc gttgggatcg agcacgactc tcacgaaatc
      181 catccagtcg ccgtagctgt tggtgaagcc ccaggcgacg tgacgattgc tgccggcgac
      241 cagcgccggc atgccgggca gggtgacgcc gatcaaatcc acggtctgac cggagcggcg
      301 cggattcgga tactcgatgc gcgtgcggaa ccagatgttc ggcacgcgca gcggcaggtg
      361 catgtcgtcg gcaatcagcg cggcatgcgt ggcggtcagt gcaccgttga cggcaaatcc
      421 attgctgccg ggcttggctc tttcttccgc gtgcgctggc ggcatgtgca agaacttcgg
      481 atcggtcttg cgcaggtcga gatcgctggc gccgggcagt tccggataat tcatcggcgg
      541 tccgagtaac ggggcatccc acggtccgcc gctggcggac aggaaccgat acgccttgtc
      601 gccgagcgcg gacttgatct gcgcgaacgc gagttcgcgc gcattgtcgg cgccgttgag
      661 gtcgaagaac atcgcgtcga tggcgaggat gcaatcggca tcgctccacg gtgcgggttt
      721 ttcgccgagc agccagtatt cccagggtcg cgaggttagg gcggcgagtc cggcattgac
      781 gccgtcgcga taggcatcga gcgttgcgcg tctgtccggc gttgcggtgg ccagcgcggc
      841 cagcgcacgc tcgcgcatgc ggaacggccg gtgctgcttg tccaggtcca cggcggcgcc
      901 gccaacgagt tcggcgagtt cgccggcggc catgcgccgc atcaggtcca tctcgaagaa
      961 gcgttcctgc gcgtgcacga agccgagcgc gaacatcgcg tcgttgcggc tttgcgcctt
     1021 tatcgtgacg acacccagtg cgtcgcgcgc aatcgtcgtt ggcgcatgga ctccgccaag
     1081 tgcgcgcgtg ccgtcgagtt ggggcaggct ggcgcgaagc agcagccacg cgaccagtgc
     1141 cagacccgcg gcgaggataa tgagtgcgcc gagcaggcgc cggatgatgc ggccagagcg
     1201 tttaccggtt ttcatttcgg ggatcagcat ccatggaatt ggtgcggggg aaggtgcgct
     1261 gtctgtgcac cctttcgcgg caaagccggc aggtattgta tcgttgtgcg tgcatcgcaa
     1321 gaccagaccg ggttccccgc tgccggatcg cgtgatgacc gagagcgatt ttctggatct
     1381 tgcacgcgcg ctgtagaatg ctgtcgatca tccgtccgat gaaggggaaa acatgcactt
     1441 tgatcgcctt gccggcgcgc ggcgcgcgcc gcgtgtccgt atgcttgcgt cccgaccccg
     1501 cttgttggcg gctggcgcca ccttgctgat catgtcgtgg acgctgacgg tttcggccgc
     1561 ggtgtgttac gtcaacggca cagcgagcgg cgccaacacg ggcgcgtcgt ggaccgatgc
     1621 ctacaccgat ctgcaatcgg ccttgaacaa cgccagttgc accgaagtct gggtggccca
     1681 gggcatctac aaaccggccg ccagcaacac ggcggtcagc ttcaatatcg ggcccaacgt
     1741 tgcggtctac ggcggttttt ccggcagcg
//
LOCUS       DASRVO010000547         1125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_249596, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000547 DASRVO010000000
VERSION     DASRVO010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249596"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VFF05_06810"
     CDS             complement(<1..1075)
                     /locus_tag="VFF05_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HZW51551.1"
                     /translation="MLLRSEGEMRPRSVFTIHNAAFQGNVALSAAQMLGLDADASTLA
                     GIEYYGQMSFLKSGTVFADKITTVSPTYARELRTPEYGCGMHGVFENRAADLVGIMNG
                     IDTEVWNPARDEHLAQCYSSGDRNGKRECKVALQASLGLQVDENAPLAISASRLTMQK
                     MADVLLRELPDLMRRHPGMQFALLGCGDRGLERGFEEFAREYPGRVAVEIGYREAAAH
                     RLHAGADILLHGARFEPCGLAQLYAMQYGTIPVVRRVGGLADSVFDANSGSSTANGFV
                     FDDCTGAALRRAVDRCLAMYAGDRGAWNALTDRAMLADFSWGRSAENYANLFFELAGK
                     AQSETLRAANSGNNERLTAWNARC"
BASE COUNT          214 a          376 c          346 g          189 t
ORIGIN      
        1 ggcaacgcgc attccacgcc gtcaggcgtt cgttgtttcc cgaattggcg gcgcgcaaag
       61 tctcgctctg cgccttgccg gccagctcga aaaacagatt cgcgtaattc tccgccgacc
      121 ggccccaact gaagtcggcc agcatcgcgc ggtcagtcaa cgcattccag gcgccgcgat
      181 cgccggcata catcgccaga cagcgatcga ccgcccgccg gagcgcggcg ccggtacagt
      241 cgtcgaacac gaacccgttt gcagtcgacg atccggaatt cgcgtcgaag acgctgtcgg
      301 caaggccacc gacacggcgt acgacaggaa tcgttccata ttgcatggca taaagctggg
      361 ccaacccgca cggttcgaag cgcgcgccgt gcaacaggat gtcggcgccc gcatgcagcc
      421 ggtgcgcggc cgcctcgcga tagccgatct cgaccgccac ccgacccgga tactcgcgcg
      481 cgaattcctc gaagccgcgc tcgagtccgc gatcgccgca gccaagcagg gcgaactgca
      541 tgccgggatg gcgccgcatc aggtcaggca gttcacgcaa caatacgtcg gccatcttct
      601 gcatcgtcag gcggctggcg gaaatcgcca gcggcgcgtt ttcatcaact tgcaatccga
      661 gcgatgcctg caatgcgacc ttgcattcgc gcttgccgtt gcggtcgccg gacgaatagc
      721 actgcgcgag gtgttcgtca cgcgccggat tccagacctc ggtgtcgatg ccgttcatga
      781 tgcccaccag atccgcagcg cggttctcga aaacgccatg catgccacag ccgtattcgg
      841 gtgtgcgcag ttcgcgcgcg taggtcggac tgaccgtcgt gatcttgtcg gcgaagacgg
      901 tgccgctctt gaggaacgac atctgcccgt aatactcgat gccggccagc gtggaagcgt
      961 ctgcatcgag gccgagcatc tgcgcggcgg acagcgccac attgccctga aaagcggcgt
     1021 tgtgaatggt gaagacgctg cgcgggcgca tctcgccctc ggaccgcaac agcaagggaa
     1081 tcagaccggt gtgccagtcg ttgcaatgca cgacgtccgc gcgcc
//
LOCUS       DASRVO010000548         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_249597, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000548 DASRVO010000000
VERSION     DASRVO010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249597"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..>1018
                     /gene="dnaX"
                     /locus_tag="VFF05_06815"
     CDS             61..>1018
                     /gene="dnaX"
                     /locus_tag="VFF05_06815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZW51552.1"
                     /translation="MSYQVLARKWRPRNFSSLVGQEHVVRALTHALEQQRLHHAYLFT
                     GTRGVGKTTLARILAKALNCETGITATPCGVCSACTEIDSGRFVDLIEIDAATNTGID
                     DMRELQDNAVYAPTRGRFKVFVIDEVHMLSKAAFNSMLKTFEEPPEHIKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPREAIYDHLGHILQQENSAAEPAALRLIAQAAQGSMRDAL
                     SLLDQAIAYAGGKVSEEAVRAMLGAIDQDYLFKLLDALARQDVKAMLAVAGDMESRSL
                     SFDSALQDLATLLHQIALAQSAPGALGQDLPERERILALAQRF"
BASE COUNT          194 a          330 c          315 g          179 t
ORIGIN      
        1 ccggctcgcc actccagaat cctttctccg aagtcgccgc gacgctggta aaatttgcac
       61 atgagttacc aggtcctcgc gcgcaagtgg cgcccgcgta atttctccag tttggtgggg
      121 caggagcatg tggtgcgcgc gctcacgcat gcgctggagc agcaacgcct gcatcacgcc
      181 tatctgttca ccggcacgcg cggcgtgggc aagacgacgc ttgcgcgcat tctggccaaa
      241 gcactcaatt gcgaaaccgg catcaccgcc acgccctgcg gcgtttgctc cgcctgcacc
      301 gaaatcgaca gcggccgatt cgtcgatctg atcgaaatcg acgccgccac caataccggc
      361 atcgacgaca tgcgcgaact gcaggacaac gccgtgtatg cgccgacgcg cgggcgcttc
      421 aaagtgttcg tgatcgacga agtgcacatg ctgtccaagg cggcgttcaa ttcgatgctg
      481 aagaccttcg aggagccgcc cgagcacatc aaattcatcc ttgccaccac cgatccgcag
      541 aaaattccgg tcacggtgct gtcgcgctgc ctgcagttca acctcaagca aatgccgcgc
      601 gaggcgatct acgaccatct cggccatatt ctgcagcagg agaacagcgc cgccgagccg
      661 gcggcgctgc ggctgatcgc ccaggccgcg cagggcagca tgcgcgacgc gctgtcgctg
      721 ctcgatcagg cgatcgcata tgccggcggc aaagtgagcg aggaagcggt gcgcgccatg
      781 ctcggcgcga tcgatcagga ttatttgttc aagctgctcg atgctttggc gcggcaggac
      841 gtgaaggcga tgctcgctgt cgccggtgat atggagtcgc gcagcctttc cttcgacagc
      901 gcgctgcagg atctggctac actgctgcat cagatcgcgc tggcacagag cgcacccggg
      961 gcgctgggcc aggacctgcc ggagcgcgaa cgcatcctgg cgctggcgca gcggttcg
//
LOCUS       DASRVO010000549         1822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_93213, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000549 DASRVO010000000
VERSION     DASRVO010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93213"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="VFF05_06820"
     CDS             <1..1731
                     /locus_tag="VFF05_06820"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HZW51553.1"
                     /translation="NRELYAAVKAVSDGTEFHGLPRAARTLVEHGLRDFRLSGVALEE
                     PARTRFRDISNALTRLATEFEEAVLDATQAWTEKVDAAALAGLPETDLALMRETAREA
                     GHDGFLVTLKPPSVQAVLTYADDRGLRERVYTAYHTRASDQGPQAGRFDNSARIGEIL
                     ALRHEAARLLGFANAAEESLATKMAATPARVLDFLHDLVRRAKPVAMRELAELREFAK
                     AKLGIADLQPWDVGYAAEKLRQRKFSLSEEDLKPYFPLPAVMDGLFAIAARVFGVSLR
                     ERAGVQVWHPDVRFYDVLDANGRVFAGCYVDLYARTGKRGGAWMDVCRSRFRNGDATH
                     PPIAYLTCNFPPPAADRQSLLAHDEVTTLFHEFGHGLHHLLTEVDYPSVAGIDGVEWD
                     AVELPSQFMENFCWQREALNLFARHWQSGEPLPDELFARMLAARHFHAGLFLVRQLEF
                     ALFDFRLHLEFDPARGARALELLADVRSEVAVLLPPDWQRMPHSFTHVFAGGYAAGYY
                     SYLWAELLSADAFARFEESGVFDAATGAAFRNSILAVGGSRPALESFVEFRGREPQPD
                     ALLRGYGLAA"
BASE COUNT          298 a          623 c          582 g          319 t
ORIGIN      
        1 aaccgcgaac tttatgccgc ggtcaaagca gtttccgacg gcaccgaatt ccacggcttg
       61 ccgcgtgccg ccagaaccct ggtcgaacac ggtctgcgcg acttccgttt gtccggcgtg
      121 gcgctggagg aaccggcgcg cacgcgcttt cgcgacatca gcaacgccct cacgcgactc
      181 gccaccgaat tcgaggaagc cgtgctcgac gcgacacagg cgtggaccga aaaagtcgac
      241 gccgcagcgc tcgccggcct gccggaaacc gacctcgcgc tgatgcgcga gaccgcgcgc
      301 gaagccggtc acgatggttt cctcgtcacg ctcaagccgc cgagcgtgca ggcggtgctg
      361 acctatgcag acgaccgcgg gttgcgcgag cgcgtctaca ccgcttacca cacgcgcgcc
      421 tcggaccagg gtccccaggc cgggcgtttc gacaattccg cgcgcatcgg ggaaatcctc
      481 gcgctgcgcc acgaagccgc gcgcctgctc ggcttcgcca atgcggcgga agaatcgctg
      541 gcgacgaaga tggccgcgac gccggcgcgc gtgctggatt tcctgcacga cctggtgcgc
      601 cgcgcgaagc ctgtcgcgat gcgcgaactg gcggaactgc gcgaattcgc caaggccaag
      661 ctcggcatcg ccgatttgca gccatgggac gtgggctatg ccgcggaaaa actgcgccag
      721 cgcaaatttt cgctgagcga ggaagacctc aagccatact ttcccttgcc tgccgtgatg
      781 gacggcctgt tcgcaatcgc cgcgcgcgtg ttcggcgtga gcctgcgcga acgtgcgggc
      841 gtgcaggtct ggcacccgga cgtgcgtttc tacgatgtgc tcgatgccaa tggtcgcgtc
      901 ttcgccggct gctatgtcga tttgtatgcg cgcaccggca agcgcggcgg cgcgtggatg
      961 gacgtgtgcc gctcgcgatt ccgcaacggc gacgcgacgc atccgccgat tgcgtatctc
     1021 acctgcaatt tcccaccacc cgccgccgac cggcagagcc tgctcgccca cgacgaagtc
     1081 accacgctgt tccatgagtt cggccacggc ctgcatcatc tgctcaccga agtcgactac
     1141 cccagcgtcg ccggcatcga tggcgtggag tgggacgcag tcgaactgcc cagccagttc
     1201 atggagaatt tctgctggca gcgcgaggcg ttgaacctgt tcgcacggca ttggcaaagc
     1261 ggagaaccgc tgcccgacga actgttcgcg cgcatgcttg ccgcgcggca tttccacgct
     1321 ggtctgtttc tcgtgcgcca gctcgagttc gcgctgttcg attttcgcct gcacctggaa
     1381 ttcgatcccg cgcgcggtgc gcgcgcgctg gagctgctcg ccgacgtgcg cagcgaagtc
     1441 gcggtgctgc tgccgccgga ctggcagcgc atgccgcaca gcttcaccca cgtcttcgcc
     1501 ggcggctacg cggcgggcta ttacagctac ctgtgggcgg aactgctttc cgccgacgct
     1561 ttcgcgcgct tcgaggaaag cggcgtcttc gatgcggcca ccggcgccgc attccgcaac
     1621 agcattctcg ccgtcggcgg ttcgcgcccg gcgctggaat ccttcgtcga atttcgcggt
     1681 cgcgaaccgc agccggacgc gctgctacgc ggttatggcc tggcggcgta aaattcgcgc
     1741 atgaaaatcg ccacgtggaa cgtcaactcg ctcaaggtgc gcctgccgca tctgcgcgag
     1801 tggctgggaa gcgccaatcc gg
//
LOCUS       DASRVO010000550         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_16312, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000550 DASRVO010000000
VERSION     DASRVO010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16312"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFF05_06825"
     CDS             complement(<1..353)
                     /locus_tag="VFF05_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZW51554.1"
                     /translation="MCVGLKQLGEYRGASVSAAAWTPSVRLVRYAPGSAIAAHSHAEA
                     SLTVLVAGHFQDNVRGRADEHRSGCALFYPAHETHAQRFSAQGALKVQIALSAAAVDF
                     LGQQRCLGDAPCLQSA"
     gene            complement(381..>1171)
                     /gene="rsmI"
                     /locus_tag="VFF05_06830"
     CDS             complement(381..>1171)
                     /gene="rsmI"
                     /locus_tag="VFF05_06830"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZW51555.1"
                     /translation="PIGNLADLSPRAQEVLRGVDLIAAEDTRHSSTLLAQYGIATRVL
                     ALHEHNEREKSEGLVQRLREGAQIALISDAGTPLVSDPGYRLVRAAREAGIAVSPVPG
                     ACAAIAALSVAGLPSDRFVFEGFLPAKSSARRAQLQTLTGETRTVIFYESSHRIQECL
                     QDCADVLGSARHAVLARELTKLYETILDGTLADLSARVASDADQRRGEFVLLIAGADG
                     NADAALLAEGRRVFDILRRELPPSRAAKLAAEISGAPRKDLYNA"
BASE COUNT          203 a          391 c          393 g          184 t
ORIGIN      
        1 gcggactgca aacatggcgc atcgcccagg caacgctgct ggccgagaaa atcaacggct
       61 gccgcagaca gggcgatctg taccttcagc gcaccttgcg cggaaaaccg ctgggcgtgc
      121 gtctcgtgcg ccggatagaa caacgcgcag ccggatcgat gttcgtcggc gcggccgcgc
      181 acattgtcct ggaaatgtcc tgcgacgagg acggtaagcg atgcctccgc gtgactgtgc
      241 gccgcgatgg ccgaacccgg cgcataacgc accagccgca ccgatggggt ccacgccgcg
      301 gccgataccg aggcgccgcg atattcgccc agctgcttca atccgacaca catggccttg
      361 gtcgtctccg acggaaccgg tcacgcattg tacaaatcct tgcgcggcgc accgctgatt
      421 tccgcggcca gcttggctgc gcggctgggt ggcagttcgc ggcgcaggat gtcgaacaca
      481 cgccggcctt cggcaagcaa ggcagcatcc gcattgccat ccgctcccgc gatcaggagc
      541 acgaattcgc cgcggcgctg gtcggcgtcg gaggccacgc gtgccgacaa atccgccagc
      601 gtgccatcga ggatggtctc gtacagtttg gtcaactcgc gcgcgagaac ggcgtgacgc
      661 gcgctgccga ggacatcggc gcaatcttgc aggcactctt ggatgcggtg gctggactcg
      721 tagaagatca cggtgcgcgt ctcgcccgtg agcgtttgca gctgcgcgcg ccgtgccgag
      781 gacttggccg gcaggaatcc ttcgaacacg aagcgatcgc tgggcagtcc cgccaccgac
      841 aacgcggcaa tcgcggcgca agcgccggga accgggctca ccgcgatgcc cgcctcgcgc
      901 gccgcgcgca cgaggcgata gcccgggtcg ctgacgagcg gagtgccggc atcgctgatc
      961 agggcgatct gcgcgccctc gcgcaggcgc tgcacgaggc cttcggattt ttcgcgctcg
     1021 ttgtgttcat gcagcgcgag cacgcgcgtg gcgatgccgt attgcgcaag caacgtcgaa
     1081 ctgtggcgcg tatcctcggc tgcaatcaga tcgacgccgc gcaggacctc ctgcgcgcgc
     1141 ggcgatagat cggcgagatt gccgatcggc g
//
LOCUS       DASRVO010000551         1241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_170666, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000551 DASRVO010000000
VERSION     DASRVO010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170666"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /gene="aroC"
                     /locus_tag="VFF05_06835"
     CDS             <1..1089
                     /gene="aroC"
                     /locus_tag="VFF05_06835"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZW51556.1"
                     /translation="FGKLFTVTTFGESHGPAIGCVIDGCPPALEIAAEEFHRDLARRA
                     TGTSKYTSQRHEADEVEILSGVFEGRTTGTPIALLIRNTDARSKDYDEIRDVFRPGHA
                     DYTYWQKYGIRDHRGGGRASARETTMRVAAGVIAKKWLAQKFGITVRGWVAQLGPLAP
                     SAFDWSAVDANPFFWPDAAMVPELERFIDALRKSGDSIGARVNVVADNVPPGWGEPIY
                     AKLDGELASALMSINAVKGVEIGAGFASVEQRGSEHRDEMYLDGFTSNHAGGILGGIS
                     TGQAVVASIALKPASSILVPGHSVSLRGDEVEVRTKGRHDPCVGIRATPIAEAMVALV
                     LMDQALRHRAQCGDVGAIEPQIPGSRKP"
     gene            1086..>1241
                     /locus_tag="VFF05_06840"
     CDS             1086..>1241
                     /locus_tag="VFF05_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZW51557.1"
                     /translation="MTKSYKVAMAGATGAVGEALLSILAERKFPVSELVPLASERSAG
                     GKVEFAGK"
BASE COUNT          218 a          413 c          410 g          200 t
ORIGIN      
        1 ttcggaaaac tcttcaccgt caccacgttc ggcgagagcc acggcccggc catcggctgc
       61 gtgatcgacg gttgtccgcc ggcgttggaa atcgccgctg aagaattcca ccgcgacctc
      121 gcgcgccgtg cgaccggcac atcgaagtac acctcgcagc ggcacgaggc cgatgaggtc
      181 gagattcttt ccggcgtgtt cgagggccgc acgaccggca cgccgatcgc gctgctgatc
      241 cgcaacaccg acgcacgctc gaaggattac gacgagatcc gcgacgtgtt ccggcccggc
      301 cacgccgact acacctattg gcagaagtac ggcatccgcg accatcgcgg tggcgggcgc
      361 gcgtctgcgc gcgagaccac gatgcgcgtg gccgcgggcg tcatcgccaa gaaatggctg
      421 gcgcagaaat tcggcatcac ggtgcgcggc tgggtcgccc agctcggtcc gctggcgcct
      481 tcggcgttcg actggagcgc cgtggacgcc aatccgttct tctggccgga tgcagcgatg
      541 gtgccggaac tcgagagatt catcgatgcg ctgcgcaagt ccggcgattc catcggcgcg
      601 cgcgtcaatg tcgttgccga caacgtgccg ccgggctggg gcgagccgat ctacgccaag
      661 ctcgacggcg agctcgcatc cgcgctgatg tcgatcaacg cggtcaaggg cgtggaaatc
      721 ggcgccggtt tcgccagcgt cgaacagcgc ggcagcgaac atcgcgacga aatgtatttg
      781 gacggcttta cgtccaatca cgcgggcggt atcctcggcg gcatctcgac cggccaagcc
      841 gtcgtcgcgt ccatcgcgct caagccggca tccagcatcc tggtgcccgg ccacagcgtg
      901 agcctgcgcg gcgacgaggt cgaggtgcgc accaagggcc gtcacgaccc ttgcgtgggc
      961 attcgcgcca cgccgatcgc ggaagcgatg gttgcgctgg tgttgatgga ccaggccctg
     1021 cgccatcgcg cgcaatgcgg cgacgtcggc gcgatcgaac cccagattcc cggaagcaga
     1081 aaaccatgac gaagtcgtac aaggtggcaa tggccggcgc gaccggtgcc gtgggcgaag
     1141 cccttctttc gattctggcc gaacgcaagt ttccggtttc cgaacttgtc ccgctcgcca
     1201 gcgaacgctc cgccggcggc aaggtcgaat tcgccggcaa g
//
LOCUS       DASRVO010000552         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_198087, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000552 DASRVO010000000
VERSION     DASRVO010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198087"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFF05_06845"
     CDS             complement(<1..255)
                     /locus_tag="VFF05_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZW51558.1"
                     /translation="MTAATARSPAANTAATSRFLLATDLDGTFLAGSASARRRLYRLI
                     QRHPGISLAWVTGRGLESVMPLLNDHALPRPDYIICDVGAT"
     gene            564..>1573
                     /locus_tag="VFF05_06850"
     CDS             564..>1573
                     /locus_tag="VFF05_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZW51559.1"
                     /translation="MRMEKLTSRFQQALADAQSLAVGRDNNMLEPAHLMAALLDQQGG
                     STVPLLAQAGVNVPSLRTRIGQALDKLPKVAGQEGNINVSNDLTRLLNLTDKLAQQRG
                     DQFIASELFVLAALDDKGEVGRALKDSGANKANLEAAVERMRGGEKVENENAEDQRQA
                     LEKYTIDLTARAEKSKLDPVIGRDEEIRRVIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNHEVPEGLRDKRVLSLDMGALIAGAKFRGEFEERLKAVLKDLAKEEGNIILF
                     IDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQ
                     KV"
BASE COUNT          329 a          463 c          541 g          240 t
ORIGIN      
        1 ggtcgcgccg acatcgcaga tgatgtagtc cgggcgcggc agggcatggt cgttgagcag
       61 cggcatgacc gattccaggc cgcggccggt gacccaggcg aggctaatgc cgggatggcg
      121 ttggatcaaa cgatacaggc gccgacgggc gctggcgcta ccggcgagaa acgtcccgtc
      181 aagatcggta gcaagcagga aacgtgaagt ggcagcagtg tttgcggcgg gggaacgggc
      241 cgttgcggcg gtcaccaagg actccggtag gggcgcatgg ctttgcggga gctatgcgcg
      301 attttgatcc gggcaattct aacatattga tcgaattatg gaaaatatct gaaattcgac
      361 gcgaacgggg ctgcctcgcc tgtcgcgccg ggtcttgcct tgatacctcg caagcctggc
      421 cgcataaatg gcagcgcatc cgaatgggtg cgatcgattc ccgaacgcag gtcggcgcct
      481 gcgttgcgtg cctcgccccg acggctttcg gggagggccg gatcagcccg agatttcgac
      541 gcgagtactg cggaggctcc tgcatgcgaa tggaaaaact gacaagtcgc ttccagcaag
      601 cgctggcgga cgcgcagtcg ctggccgtgg gccgcgacaa caacatgctt gaaccggcgc
      661 atctcatggc cgccttgctc gaccagcagg gcggctcgac cgtgcccctg ctcgcgcagg
      721 cgggcgtgaa cgtgccgagt ttgcgcacgc gcatcgggca ggcgctggac aagctgccga
      781 aagtcgcggg tcaagagggc aacatcaatg tctccaacga cctgacgcgc ctgctcaacc
      841 tcaccgacaa gctggcccag cagcgcggcg atcaattcat tgccagcgaa ttgttcgtgc
      901 tcgccgcgct cgacgacaag ggcgaggtcg gccgtgcgct gaaggattcc ggcgcgaaca
      961 aggcgaacct ggaagcggcg gtcgaacgca tgcgcggagg tgaaaaagtg gaaaacgaaa
     1021 atgccgagga ccagcgccag gcgctggaga aatacaccat cgacctgacc gcgcgcgccg
     1081 agaaatccaa gctcgatccc gtgatcggtc gtgacgagga aatccgccgc gtcatccagg
     1141 tgctgcagcg gcgcaccaag aacaacccgg tgttgatcgg cgagccgggc gtcggcaaga
     1201 ccgcgatcgt cgagggactg gcgcagcgca tcgtcaacca cgaggtgccc gagggcttgc
     1261 gcgacaagcg cgtgctgtcg ctcgacatgg gcgcgctgat cgccggcgcg aagttccgcg
     1321 gcgagttcga ggaacggctc aaggccgtgc tgaaggatct tgccaaggaa gagggcaaca
     1381 tcatcctgtt catcgacgag ctgcacacga tggtcggcgc cggcaaggcc gaaggcgcga
     1441 tggatgccgg caacatgctg aagccggcgc tcgcgcgcgg cgaactgcat tgcatcggtg
     1501 cgacgacgct cgacgaatac aggaagtaca tcgagaagga cgcggcgctc gagcggcgct
     1561 tccagaaagt cta
//
LOCUS       DASRVO010000553         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_276423, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000553 DASRVO010000000
VERSION     DASRVO010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276423"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFF05_06855"
     CDS             complement(<1..369)
                     /locus_tag="VFF05_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51560.1"
                     /translation="MKRRTLLQLAAAVAAWAPIARLRAFAQAPPFSDEQKATLGAIAE
                     VTLPASIGSTGRVAATQKFIAWHLNYRTGADMGHGYGASTLRPASRPSVLPRYAAQCA
                     ALDAAARAQGAASFAAASIGI"
     gene            complement(545..>1170)
                     /locus_tag="VFF05_06860"
     CDS             complement(545..>1170)
                     /locus_tag="VFF05_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW51561.1"
                     /translation="LGAVPARIGDVLRSVSLTPAVREQMQTALCDAHSVEHRAANLVA
                     ALQFRDRDASFQHVPVDLTALVERVVAREGMFASACGVPLSATVPDQPVNADGDPGVF
                     ELMLVNLIDNAIRYNKPGGAVAVDLDAKPGGHFVLLVSDDGPGVNDATLKYLNAIRRF
                     RGDEHRNRRADEVGLGLAIVHEVSGRAKINLAFRRRDNGGLEVRLSQ"
BASE COUNT          194 a          390 c          393 g          193 t
ORIGIN      
        1 gatgccgatc gaggcggccg cgaacgacgc ggcgccttgc gcgcgcgcgg cggcatcgag
       61 cgcggcacac tgcgcggcat agcgtggaag aaccgacggc cgcgacgccg gacgcaatgt
      121 ggacgcgccg tagccatgcc ccatgtccgc gcctgttcga taattcagat gccaggcgat
      181 gaacttctgc gtggccgcca cgcggccagt cgatccgatg gatgccggaa gcgtgacctc
      241 ggcaatcgca ccgagcgtgg ctttctgctc gtcagagaac ggcggcgctt gcgcgaacgc
      301 ccgcagccgt gcgatcggcg cccacgccgc cacggcggcc gcaagctgca ggagcgtccg
      361 gcgtttcatc atttggcgga ttgtacggta cggacgggac gagtccaagg ttcaacgtcc
      421 aaggtccaag gtcactcgtc gtccagagtc caggacgtcc ttggtcctgg gacgtccgga
      481 catccaggac cttggacaac aagtgacgtt ggaccttgga ccttgaactt tggacttcct
      541 cgtcctactg gctcagtctc acttccaacc cgccgttgtc cctgcggcgg aacgcgaggt
      601 tgatcttggc ccgtcccgaa acctcgtgga cgatggcgag gcccaggccc acttcgtcgg
      661 cgcggcggtt gcggtgttcg tcgccacgga agcggcggat ggcgttcagg tacttgagcg
      721 tggcgtcatt cacacccggg ccgtcgtcgc tgacgagcag gacaaagtgg ccgccgggtt
      781 tggcgtcgag atcgaccgcc acggcgccgc ccggcttgtt gtagcggatg gcgttgtcga
      841 tgaggttgac gagcatcaac tcgaacacac ccggatcgcc gtcggcgttc accggttgat
      901 ccggcaccgt ggccgacagc ggcacgccgc acgcgctcgc gaacatgccc tcgcgcgcca
      961 ccacgcgttc gaccagcgcg gtcaggtcga cgggcacatg ctgaaagctc gcgtcgcggt
     1021 cccgaaactg caatgccgcc accaggttgg cggcgcgatg ttcgacggaa tgcgcgtcgc
     1081 agagcgccgt ctgcatttgc tcgcgcaccg caggtgtcag gctgaccgag cgcagcacgt
     1141 cgccgattcg cgccggcacc gcgccgagcg
//
LOCUS       DASRVO010000554         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_145433, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000554 DASRVO010000000
VERSION     DASRVO010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145433"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFF05_06865"
     CDS             complement(<1..587)
                     /locus_tag="VFF05_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51562.1"
                     /translation="MGGIEATDVIAQAKQALQRGDAAGAERQLRGLLARRPHEAEGLR
                     LLGMAQNARGDYSGAAANLRRAVEADPRNALAFNSLGSALGRLGDKPAAAEAFAKACT
                     LAPRIAQFHYNLGKVLSEDARSVEALASLREAVALDPDNLPVQFLLAHEERVTGDTQA
                     AIQRYRHLLDGHPERAEAWLGLAGMQRLRFSADDVA"
     gene            complement(574..1650)
                     /gene="rsgA"
                     /locus_tag="VFF05_06870"
     CDS             complement(574..1650)
                     /gene="rsgA"
                     /locus_tag="VFF05_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZW51563.1"
                     /translation="MQSTDEFARLKAIGWRGDTADVPAGKRLARVIAQHRNGYRVHDG
                     ATEFAAQPAPRFLRRDVDPAERPAVGDFVVLTRAAPPTIDEILPRRSLLERAAAGERH
                     RRQVIAANVDTVFVLMGLDGDFNPRRVERYLVLIEGSGARPVVVLTKIDKVENAASAA
                     MLAELHTAIPRAEMHAINAKDAASVAPLLKFLQPGDSAVLVGSSGAGKSTLTNTLLGV
                     ERHATAAVRGHDSRGRHTTTYRALVQLPSGGCLIDTPGMREIKFTGDEELGAAANFSD
                     IETLALDCRFGDCGHGNEPGCAVRAALESGDLDPSRWQAWLKLRDELAAAGDALEAQL
                     KRKSAARVANKALGKRLAEKYGRN"
     gene            1743..>2987
                     /locus_tag="VFF05_06875"
     CDS             1743..>2987
                     /locus_tag="VFF05_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZW51564.1"
                     /translation="MSVPLPHPPVRANPRLAQVRYDIRGELSRRARELESAGRDIVHL
                     NIGNPGRFGFAAPEHLRRAVAEHVSDSEAYCPQQGLLPAREAIAARELARGAHAASAA
                     NVFVGNGVSELIDMSLRALLDAGDEVLLPSPDYPLWSAAVALNGGNARYYACPAARGH
                     LPDPDEIEALITPRTHALVVINPNNPTGTVYPQELLAALVRIAQRHRLVLLSDEIYDD
                     IVYDEARCVPLAPLADEVPTLTFGGLSKVHRACGWRVGWVSLSGARDALAEIHHALDL
                     LASLRLCANVPGQWAIAPALAGPDTISALTAPGGRLHEARRAVVEHCAQSEFLEIVAP
                     QGALYAFPSVRTEVFPDFDDNTFALQLLETENVLIVPGRGFNIVEANHFRATLLPPPA
                     QLFEVFARIENCLERMAASSRRH"
BASE COUNT          470 a         1042 c          955 g          520 t
ORIGIN      
        1 gccacgtcgt ccgccgagaa acgcaggcgc tgcattccgg ccaatccaag ccaggcctcg
       61 gcgcgctcgg gatggccgtc gagcaaatgc cggtagcgct ggattgcagc ctgggtgtcg
      121 ccggtaacgc gctcttcgtg cgcaagcagg aactgcaccg gcaggttgtc cgggtcgagc
      181 gctactgcct cgcgtagaga agccagcgct tccacggaac gcgcgtcctc gctcaaaacc
      241 ttgccaaggt tgtaatgaaa ttgcgcgatt cgcggtgcaa gtgtacacgc cttggcaaat
      301 gcttccgccg ccgccggctt gtcgccaagg cgacccaacg cgctgcccag actgttgaag
      361 gcgagcgcgt tgcgcggatc ggcctcgacc gcgcggcgca aattcgctgc cgcgccgctg
      421 taatcgcccc gcgcgttttg cgccatacca agcaggcgca agccttccgc ctcatgtggg
      481 cggcgtgcga gcagcccgcg caattgtcgt tcggcccccg ccgcgtcgcc gcgctgtagc
      541 gcctgcttcg cctgcgcgat gacatcggtc gcttcaattc cgcccatatt tctccgccag
      601 gcgcttgccc agcgccttgt tcgccacgcg cgcggcggac ttgcgcttga gttgcgcttc
      661 gagcgcatcg cccgccgcag ccagttcgtc gcgcaatttc agccaggcct gccagcgtga
      721 cgggtcgagg tcgccggatt ccagcgccgc acgcaccgcg caaccgggtt cgttgccgtg
      781 gccacagtcg ccgaagcggc agtccagcgc cagcgtctcg atgtcggaaa aattcgccgc
      841 cgcgccgagt tcctcgtcgc cggtgaactt gatctcgcgc atgcccggcg tgtcgatcag
      901 gcaaccgccg ctgggcaatt gtaccaatgc gcggtacgtg gtggtgtgac ggccgcggct
      961 gtcgtggccg cgcacggccg cggtggcgtg gcgttcgacg ccgagcaggg tattggtcag
     1021 cgtcgatttg ccggcgccgg acgatcccac cagtacggcg ctgtcgccgg gttgcagaaa
     1081 cttcagcaac ggcgcgacgc tggccgcgtc cttggcattg atcgcgtgca tttctgcccg
     1141 tgggatcgcc gtgtgcaatt ctgcaagcat cgcggcagat gcggcatttt ccactttgtc
     1201 gattttcgtc agcacgacga ccggccgtgc gccgctgcct tcgatgagca cgaggtagcg
     1261 ttcgacccgg cgcggattga agtcgccgtc cagtcccatc agcacgaaca cggtgtcaac
     1321 attcgcggcg atgacctggc gacgatggcg ttcgccggcg gcggcacgct cgagcaggct
     1381 gcgccgcggc aggatctcgt cgatcgtcgg cggcgccgcg cgggtgagca cgacgaaatc
     1441 ccccaccgcc gggcgttcgg ccggatcgac gtcgcgacgc aggaagcgcg gcgccggctg
     1501 cgcggcgaac tcggtcgcac cgtcatgcac gcgatagccg ttgcgatgct gggcgatgac
     1561 gcgcgccagg cgctttccag ccggcacatc cgcggtgtcg ccgcgccagc cgatggcttt
     1621 gaggcgggcg aattcgtcgg tcgattgcat ccggccattt tatcctgccc ccgcgcatat
     1681 tccttgaatt cacgggcgat tgcgttagca tgctgcgtta caacaatttc tctaccccga
     1741 tcatgtccgt gcccctgccc cacccgccgg tccgcgccaa tccgcggctg gcacaagtgc
     1801 gttacgacat ccgcggcgag ctgtcgcggc gcgcgcgcga actcgaatcc gccgggcgcg
     1861 acatcgtgca cctgaacatc ggcaaccccg gacgcttcgg tttcgccgcg cccgaacacc
     1921 tgcgccgcgc cgttgccgaa catgtatccg acagcgaagc gtattgcccg cagcaagggc
     1981 tgttgcccgc gcgcgaagcg atcgccgcga gggaactcgc acgcggcgcg catgctgcgt
     2041 ccgctgcgaa tgtcttcgtc ggcaacggcg tcagcgaact catcgacatg agcctgcgcg
     2101 ccctgctcga cgccggcgac gaagtgttgc tgccgagtcc ggattatcca ctgtggagtg
     2161 ccgcggtcgc gctcaacggc ggcaacgcac gctactacgc ctgccccgcc gcacgcggac
     2221 atcttcccga tcccgacgag atcgaggcgc tgatcacgcc gcgcacgcat gcgctggtcg
     2281 tgatcaatcc gaacaatccg accggcaccg tgtatccgca agaactcctc gccgcgctgg
     2341 tgcgcatcgc gcaacgccac cggctcgtgc tgctcagcga cgaaatttac gacgacatcg
     2401 tttacgacga agcgcgctgc gtgccgctcg cgccgcttgc cgacgaggta cccacgctca
     2461 cattcggcgg actttccaag gtgcaccgcg cctgcggctg gcgcgtgggc tgggtcagcc
     2521 tttccggcgc acgcgacgcc ttggccgaga ttcaccacgc gctggacctg ctcgcgagcc
     2581 tgcgcctgtg cgcaaacgtg ccggggcagt gggcgatcgc gccggcgctg gccggcccag
     2641 acacgatctc ggcactgacc gcgcccggcg ggcgtctgca tgaagcgcgc cgcgccgtcg
     2701 tggaacattg tgcgcaaagc gaattcctgg aaatcgtcgc accgcagggc gcgctatacg
     2761 cgtttccatc cgtgcgcacc gaagtgttcc cggatttcga cgacaacacc ttcgcgctgc
     2821 agctgcttga aaccgaaaac gtgctgatcg tgccgggtcg cggtttcaac atcgtcgagg
     2881 cgaaccactt ccgcgccacg ctgttgccgc cgccggcaca gttgttcgag gtattcgcgc
     2941 gcatcgaaaa ctgtctggaa cgcatggccg cgtcgagccg gcggcat
//
LOCUS       DASRVO010000555         1320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_379243, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000555 DASRVO010000000
VERSION     DASRVO010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379243"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFF05_06880"
     CDS             complement(<1..696)
                     /locus_tag="VFF05_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZW51565.1"
                     /translation="MKLPLDKIAGYIQAVGEFDGDSLAHGYSIDSRTIAPGELFFAVR
                     GERVDGHDYVEAALQNGAVAAVVSQGMAARYPDRRKLLLVEDPLAGLQRLGASVRSAW
                     GRPVIAVTGSAGKTTTKEIIARLLATGYRVLKSEGNLNNHFGLPLQLLRLEPEHELAV
                     LELGMSHAGEITLLASLAQPNLGLVTNVAPVHLGFFKSVAEIARAKQELIQALPLQAT
                     AMLNADDEYVANFG"
     gene            complement(693..1301)
                     /locus_tag="VFF05_06885"
     CDS             complement(693..1301)
                     /locus_tag="VFF05_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZW51566.1"
                     /translation="MLAAAAAAFARGCTPEAIVAGIDQTPRVPGRFETIHAGQPFTVV
                     VDYAHTDDALRNLTALARELVAREGGRGRVILVFGCGGDRDRSKRPLMGEAAGQGSDL
                     VVLTSDNPRSEEPLAIMNDVLPGLQRSGVAYVLEPDRRKAILRAIDSAQAGDIVLLAG
                     KGHEKTQISAAGSVPFDDVEAARAALAAIGYAERQPRREVTG"
BASE COUNT          223 a          457 c          418 g          222 t
ORIGIN      
        1 gccgaaattc gccacatatt catcgtcggc attcagcatg gcggtcgcct gaagcggcag
       61 cgcctgaatc agttcctgct tggcgcgcgc gatctccgcc accgatttga aaaagcccag
      121 atgcaccggg gccacattgg tcaccagccc aagattcggc tgggccaggc tggccaacag
      181 cgtgatctcg ccggcatggc tcatccccag ttctaaaacc gccagctcgt gctccggctc
      241 caggcgcagc aattgcagcg gcaggccaaa gtggttgttc aggttgcctt ccgatttcag
      301 cacccggtag ccggtcgcca gcaggcgagc gatgatttcc ttggtggtgg tcttgcccgc
      361 cgagccggtc acggcgatca ccggacggcc ccaggcgcta cggacactgg cgcccagccg
      421 ctgcaacccg gcgagcggat cttcgaccag cagcagcttg cgccgatcgg gataacgggc
      481 cgccattccc tggctgacga cggcggcgac ggcgccgttc tgcagcgccg cttccacata
      541 atcgtggcca tcgacgcgct cgccgcgcac cgcaaagaac agttcgccgg gcgcgatggt
      601 gcgcgagtca atggaatagc cgtgggccag cgaatcgcca tcgaactcgc ctacggcttg
      661 gatgtagccg gcaatcttgt ccaggggaag tttcatccgg tgacctcacg gcgcggctgc
      721 cgttcggcat agccgatcgc ggccagcgcc gcgcgcgccg cctccacgtc atcaaagggg
      781 accgaaccgg cggcgctgat ttgcgttttt tcgtgaccct tgccggccag cagcacgatg
      841 tcgccggctt gcgcgctatc gatggcgcgc aggatcgcct tgcgccggtc cggctccagc
      901 acgtaggcaa cgccgctgcg ctgcaatccg ggcagcacgt cattcatgat ggccagcggc
      961 tcctcgctgc gtggattgtc ggaggtaagc acgaccaaat cgctgccctg tccggcggcc
     1021 tcccccatca acggacgctt gctgcggtcg cgatcgccgc cgcagccaaa gaccaggatg
     1081 acgcgtccgc gcccgccctc acgggccacc aactcgcggg ccagcgccgt caagttgcgc
     1141 aacgcatcgt cggtatgggc gtagtcgacg accacggtga acggctggcc ggcatggatg
     1201 gtttcaaagc ggccgggcac gcgcggcgtc tggtcgatgc ccgcgacaat cgcttccggc
     1261 gtgcagccgc gggcgaacgc cgccgccgcc gcggccagca cgttatatac gttcactttg
//
LOCUS       DASRVO010000556         1137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_379307, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000556 DASRVO010000000
VERSION     DASRVO010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379307"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFF05_06890"
     CDS             complement(<1..795)
                     /locus_tag="VFF05_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HZW51567.1"
                     /translation="MTYLTKPKFHHPELPKNELGYTHRDYEGTVSTLCAGCGHDSITA
                     SIIQACFELSIEPHRVAKISGIGCSSKTPTYFLGNSHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGFGQFAHALRRGVNMAYIVENNGVYGLTKGQFSATADRGSKS
                     KRGFINTDNSIDLVAMALQLGATFVARSFSGDKHQLVPIIKAAIAHKGAAFIDVISPC
                     VAFNNHAGSTKSFDFVRAHNEAVNRLDYVTAREPIAVDYEPGTVEAV"
     gene            complement(792..>1137)
                     /locus_tag="VFF05_06895"
     CDS             complement(792..>1137)
                     /locus_tag="VFF05_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZW51568.1"
                     /translation="KYGVVYFGSTAPAMEEAIALIEARGHAVDRLRVRAFPFHSSVNS
                     FVAGHDFVFVIEQNRDAQLRSLIVNECGIDPVRLVPILHYDGTPITARFIAKAIGDHV
                     DKLKVTPLKVSS"
BASE COUNT          209 a          330 c          393 g          205 t
ORIGIN      
        1 cacggcttcg accgtacccg gctcgtaatc gaccgcgatc ggttcccgcg cggtgacgta
       61 atcgagccgg ttcaccgcct cgttgtgcgc gcgcacgaaa tcgaagctct tggtcgagcc
      121 ggcgtgattg ttgaaggcga cgcagggcga gatcacgtcg atgaaggcgg cgcccttgtg
      181 cgcgatcgcc gccttgatga tcggcacaag ctggtgcttg tcgccggaga aggagcgggc
      241 gacgaaggta gcgccgagct gcagcgccat ggcgaccagg tcgatcgaat tgtcggtgtt
      301 gatgaagccg cgcttggact tggagccgcg gtcggcggtg gcggagaact ggcccttggt
      361 gaggccatag acgccgttgt tctcgacgat ataggccatg ttgacgccgc ggcgcagcgc
      421 atgggcgaac tggccgaagc cgatcgaggc gctgtcgccg tcgccggaga cgccgagata
      481 gatcaggtcg cggttggcca ggttggcgcc ggtcaggacc gacggcatgc ggccgtgcac
      541 cgaattgaaa ccgtgcgaat tgccgaggaa ataagtcggc gtctttgacg agcaaccgat
      601 gccggaaatc ttcgccaccc ggtgcggctc gatcgacaat tcgaagcagg cctggatgat
      661 cgaggcggtg atcgaatcgt ggccgcagcc ggcgcacagc gtcgacaccg tgccctcgta
      721 gtcgcggtgg gtgtagccga gctcgttctt cggcagctcg gggtgatgga atttcggctt
      781 ggtgaggtag gtcatgagga taccttcagc ggcgtcactt tcagcttgtc gacatgatcg
      841 ccgatcgcct tggcgatgaa gcgcgcggtg atcggcgtgc cgtcatagtg caaaattggc
      901 accagccgca ccggatcgat gccgcattcg ttgacgatca gcgagcgcaa ttgcgcgtcg
      961 cggttctgct cgatcacgaa cacaaaatcg tggccggcga cgaagctgtt gaccgacgaa
     1021 tggaacggga aggcgcgcac gcgcagccgg tcgaccgcat gcccgcgcgc ctcgatcagc
     1081 gcgatcgcct cttccatcgc cggcgcggtc gagccgaaat agaccacgcc gtatttg
//
LOCUS       DASRVO010000557         1257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_198306, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000557 DASRVO010000000
VERSION     DASRVO010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198306"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1257
                     /locus_tag="VFF05_06900"
     CDS             <1..>1257
                     /locus_tag="VFF05_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014508762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HZW51569.1"
                     /translation="AVPDFSAGAMENAGLVTFRDWLLLLDPDSAQRYVQGSFDVTAHE
                     LAHQWTGDTVTMTWWNDLWLNEAFATWMQQKITGKVHPEYRADLARVQGAQGAMANDS
                     LVSARKIRQPITGNGDIQTAFDNITYRKGAAVLGMFEAFVGEDTFRNGMRAYIQEHKF
                     SNASADDLIDAIAKAADKGVKFKQAFGSFLDQSGVPMVTTQLDCSGKQPVLKLAQSRY
                     LPLGSTGSTDRVWGVPMCVRTPAGTRCELFDAATGSMALADARCPAWYMPNAGARGYY
                     RFSMAAKDLAKLNGALDQLSDAEKLAYADAIDAAFRRGDIDAHDVLAAVRELAPSKTT
                     QVALAPLSTLEWIWHHEAQTDAQKARLRNVAKAAYLPRLQALGYARKAGEPVEDTQMR
                     SSLAGTLGWTLKLPEVRAALLAQGDAV"
BASE COUNT          245 a          428 c          397 g          187 t
ORIGIN      
        1 cgcagtgccg gatttcagcg cgggcgcgat ggaaaacgcc ggcctggtca cgttccgcga
       61 ctggctgctg ctgctcgatc cggattccgc gcagcgctac gtgcaaggct ccttcgacgt
      121 gaccgcgcac gaactcgcgc accagtggac cggcgacacc gtcaccatga cctggtggaa
      181 cgatctgtgg ctgaacgaag ccttcgccac ctggatgcag cagaaaatca ccgggaaagt
      241 gcatccggaa taccgcgccg acctcgcccg cgtgcaaggc gcgcaaggcg caatggccaa
      301 cgactccctg gtcagcgcgc gcaagatccg ccagccgatc accggcaatg gcgacatcca
      361 gaccgccttc gacaacatca cctaccgcaa aggcgccgcg gtgctgggca tgttcgaggc
      421 ctttgtcggc gaagacacgt tccgcaacgg catgcgtgcc tacatccagg aacacaaatt
      481 cagcaacgcc agcgccgatg atttgatcga cgccatcgcc aaggcggcgg acaagggcgt
      541 gaaattcaag caggcgttcg gcagcttcct cgaccagtcc ggcgtgccga tggtgacgac
      601 gcaactcgat tgcagcggca agcagccggt attgaaactg gcacagagcc gctacctgcc
      661 actcggttcc acgggcagca ccgatcgcgt atggggcgtg ccgatgtgcg tgcgcacgcc
      721 ggctggcacg cgttgcgagc tgttcgacgc cgccacgggc agcatggcac tcgccgatgc
      781 gcgttgtccg gcgtggtaca tgcccaatgc cggtgcgcgc ggctactacc gcttcagcat
      841 ggcggcgaaa gacctggcca aactcaacgg cgcgctcgac cagctcagcg acgccgagaa
      901 gctggcgtat gccgatgcca tcgatgccgc cttccgccgc ggcgacatcg atgcccacga
      961 cgtgctcgcg gccgtgcgcg agctggcccc gtccaagacc acgcaggtgg cattggcgcc
     1021 cttgtccacg ctcgaatgga tctggcacca cgaggcacag accgacgcgc agaaagcacg
     1081 cctgcgcaac gtggccaagg ccgcctatct gccgcgcctg caagcgctcg gttatgcgcg
     1141 taaggccggt gaaccggtgg aagacacgca gatgcgcagc tccttggccg gcacgctcgg
     1201 ctggacgttg aaactgcccg aggtacgcgc cgcgctgctc gcacaaggcg atgccgt
//
LOCUS       DASRVO010000558         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_302697, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000558 DASRVO010000000
VERSION     DASRVO010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302697"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /gene="purF"
                     /locus_tag="VFF05_06905"
     CDS             complement(<1..1035)
                     /gene="purF"
                     /locus_tag="VFF05_06905"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZW51570.1"
                     /translation="MALRKQLVHEGAMMQSTTDTEVILHLVARARRNRFVDRFVEGLR
                     QLEGAYAFVGLTNKKLVGARDPLGIRPLVIGKLDGCPILASETCALDIIGATYVRDVE
                     NGEVVVFDESGMQTHKPFPPQEPRPCVFEYIYFSRPDSIVGGRHVYDVRKGMGAELAR
                     EAPAQADVIVPVPDSGVPAGLGFAQASGLPYELGIIRNHYVGRTFIQPTQAIRDQGVR
                     LKHSANRAVVNGKRIVLVDDSIVRGTTSRKIVQMMRDAGAQEVHFRIASPPITHPDYY
                     GIDTPERDKLLAATHDLEGMRQFIGADSLAFLSVDGIYRAMGYEGRDPARPQFTDHCF
                     TGDYPTPLTDL"
BASE COUNT          171 a          331 c          360 g          178 t
ORIGIN      
        1 gagatcggtg agcggggtcg gatagtcgcc ggtgaagcag tgatcggtga attgcgggcg
       61 cgcgggatcg cgcccttcgt agcccatggc gcggtaaatg ccgtcgaccg acaggaaggc
      121 cagcgaatcc gcgccgatga attgccgcat gccttcgaga tcgtgcgtcg ccgccaacag
      181 cttgtcgcgc tcgggcgtgt cgatgccgta atagtcggga tgcgtgattg gcggcgaggc
      241 gatgcggaaa tgcacttcct gggcgccggc gtcgcgcatc atctgcacga tcttgcgcga
      301 ggtggtgccg cgcacgatcg aatcgtcgac cagcacgatg cgcttgccgt tgaccaccgc
      361 gcgattggcc gagtgcttca gccggacgcc ctggtcgcgg atcgcctgcg tcggctggat
      421 gaaggtgcgg ccgacataat ggttacggat gatgccgagc tcgtatggca gcccggaggc
      481 ctgcgcgaag ccgagccccg ccggcacgcc tgagtccggc accggcacga tcacgtcggc
      541 ttgcgccggc gcttcgcggg cgagctcggc gcccatgccc ttgcgcacgt cgtagacgtg
      601 ccggccgccg acgatggaat ccggccgcga gaaatagatg tattcgaaca cgcaggggcg
      661 cggctcctgc ggcgggaacg gcttatgcgt ctgcatgccg ctctcgtcga agacgaccac
      721 ttcgccgttc tcgacgtcgc gcacataggt cgcgccgatg atgtcgagcg cgcaggtttc
      781 cgacgccagg atcgggcagc cgtcgagctt gccgatcacc agcgggcgga tgccgagcgg
      841 gtcgcgcgcg ccgaccagct tcttgttggt cagcccgacg aaggcatagg cgccttcgag
      901 ctggcgcagc ccttctacga aacggtcgac gaaacggttg cgacgcgccc gcgccaccag
      961 atgcaggatc acttccgtat cggtagtcga ctgcatcatg gcgccttcgt gcaccaactg
     1021 cttgcgcagc gcgatgccgt
//
LOCUS       DASRVO010000559         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_43230, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000559 DASRVO010000000
VERSION     DASRVO010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43230"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFF05_06910"
     CDS             complement(<1..407)
                     /locus_tag="VFF05_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HZW51571.1"
                     /translation="MFESLSQRLSSTIERLRGRARLTEANIRESLREVRIALLEADVA
                     LPVVQALIQRVQVRAVGQEVLKSLTPGQALVKVVRDELTAVMGTANSDLNLAAAPPAI
                     VLMAGLQGAGKTTTVAKLAKFLKDRKKKKVMVV"
     gene            494..1270
                     /gene="ccsA"
                     /locus_tag="VFF05_06915"
     CDS             494..1270
                     /gene="ccsA"
                     /locus_tag="VFF05_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZW51572.1"
                     /translation="MPIHLLAVVSAALYLCAAWLFARPPRKWMLFVAAAAVLTHAAIL
                     VGQHGGGVDLHFFAALSLVAGCVAALCLLVNLARPVATLGTIVFPLAAILLSIDVFLA
                     PHTEPLPIDWQIKLHVIFSLLGFSVLSVAAVLAILLALQEHALRARRFDGIAAALPPL
                     TLTKALMFQTIGAGFVLLTVAILIGFLFVDNLFAQHLIHKTVLSIGAWIVFGTLLLGR
                     WRYGWRGRRAVRMTLSGMILLLLAFFGSRFVLEVVLKRVP"
     gene            1302..1670
                     /locus_tag="VFF05_06920"
     CDS             1302..1670
                     /locus_tag="VFF05_06920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZW51573.1"
                     /translation="MSKPANAAERRRFQRFHFEGTVKLYSDKAMWESKLDDISLKGVL
                     IEHPPGWNGKIGSSYRMDLRINNSVIISMGVVAAHIMPHRIGFQWQKIDLDSFAELKR
                     LVELNLGDPEILNRELSALG"
     gene            complement(1912..>2595)
                     /locus_tag="VFF05_06925"
     CDS             complement(1912..>2595)
                     /locus_tag="VFF05_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HZW51574.1"
                     /translation="LEHMVDAVLYFEGESGSRFRVLRAFKNRFGAVNELGVFAMGDKG
                     LREVPNPSAIFLSAHAGPTPGSAVMVTREGTRPLLVEVQALVDQSSLGNPRRVALGLE
                     QNRLAMLLAVLHRHGGMAVYDQDVFVNVVGGIRVQETAADLPVLLAVLSSFRDRPLPE
                     KTVAFGEVGLSGEIRPVPNGEERLKEAATHGFLKAIVPKANAPKKSRIGEMEIVAVER
                     LAEAIAAVI"
BASE COUNT          438 a          883 c          771 g          503 t
ORIGIN      
        1 ctcacgacca tcaccttttt cttcttgcga tccttgagga acttcgcgag cttggcgacc
       61 gtcgtcgtct tgcccgcacc ctgcaagccc gccatcagca cgatggctgg cggcgcggcg
      121 gcaagattca ggtcggaatt cgccgtgccc atcaccgcgg tcaattcatc gcgcacgacc
      181 ttgaccaggg cctggcccgg cgtgagcgac ttcagcactt cctggccgac cgcgcgcacc
      241 tgcacgcgct ggatcagcgc ctgcacgacc ggcagcgcga cgtcggcctc gagcaaggca
      301 atgcgcacct cgcgcagcga ttcgcggatg ttggcctcgg tcaggcgcgc gcgcccgcgc
      361 aggcgttcga tggtggatga caggcgctgg ctcagggatt cgaacatgat gcgttacggc
      421 aatcttggga acttcacagt ataccagcgc cgcatcgttc cttcgccgcg ccggctatgc
      481 gacactggca tccatgccga ttcatctgct cgccgtcgtc tctgctgcgc tgtacctgtg
      541 cgcggcatgg ctgttcgcgc gcccgccgcg caaatggatg ttgttcgtcg ccgccgcggc
      601 ggtgctcacg cacgccgcga tcctggtcgg ccagcacggc ggcggcgtgg acctgcactt
      661 cttcgccgcg ctgtcgctgg tcgccggctg cgtcgccgca ctgtgcctgc tggtgaatct
      721 ggcgcgaccc gtcgcaaccc tcggcacgat cgtgttcccg ctcgccgcga tcctgctgtc
      781 gatcgacgtg tttctcgccc cgcacaccga accgctgccg atcgactggc agatcaagct
      841 gcacgtcatt ttttccctgc tcgggttttc agtactcagc gtcgccgccg tactggcgat
      901 cctgctcgcc ctgcaggagc acgccctgcg cgcgcgccgc ttcgacggca tcgccgctgc
      961 cctgccgccg ctgaccctga ccaaagctct gatgttccag acgatcggcg cgggtttcgt
     1021 gcttctgacc gtggccatcc tcatcggctt cctgttcgtc gacaacctgt tcgcccaaca
     1081 tttgatccac aagaccgtgc tcagcatcgg cgcctggatc gtgttcggca ccctgctgct
     1141 cgggcgctgg cgctacggct ggcgcggccg ccgcgcggtg cgcatgacct tgtctggcat
     1201 gatcctgctg ctgctcgcct tcttcggcag ccgcttcgtc ctcgaagtcg tcttgaagcg
     1261 cgtgccttga ttgccgctgc gccgctgtgc agaatcggca catgagcaag ccagccaacg
     1321 ctgccgaacg ccgccgtttc cagcgcttcc atttcgaagg cacggtgaag ctgtattcgg
     1381 acaaggcgat gtgggagagc aagctcgacg acatctcgct caagggcgtg ctgatcgaac
     1441 atccgcccgg ctggaacggc aagatcggct ccagctaccg catggacctg cgcatcaaca
     1501 actcggtgat catcagcatg ggcgtcgtcg ccgcgcacat catgccgcac cgcatcgggt
     1561 tccagtggca gaagatcgac ctcgacagct tcgccgagct gaagcggctg gtggaattga
     1621 acctgggcga tccggaaata ttgaatcgag agttgtcggc gttgggttga ttcttcctcg
     1681 tcattccgac gcaatccgga atccactttt tcggcatgaa agcttccact cggcctgcgg
     1741 ccgagcgggt aacttttttg ctcgtgcaaa aaagtcagca aaaaacacga tcaaagcaga
     1801 atttcccatg ggaggaactt cacgcaggcg tagcgacggg atgcaaattt cgattgcagc
     1861 ccggtcgagc aggatcgaag cacacgcttg cgagttggcg gaacggaagc ttcaaatcac
     1921 cgccgcgatc gcctcggcaa gcctctccac cgccacgatt tccatttcgc cgatgcggga
     1981 cttcttcggc gcgttcgcct tgggcacgat ggctttgaga aatccgtgcg tcgcggcttc
     2041 cttcaggcgc tcctcgccgt tcggcaccgg gcggatttcg ccggacagtc ccacttcgcc
     2101 gaacgcaacg gttttttccg gcaagggacg gtcgcggaaa ctcgacaaca ccgcgagcag
     2161 caccggcaga tccgccgccg tttcctgcac gcggatgccg ccgaccacgt tgacgaacac
     2221 atcctggtca tacacggcca tgccgccgtg gcgatgcaat accgccagca acatggcgag
     2281 gcgattctgt tcgagcccaa gcgcgacgcg gcgcggattg ccgagcgagc tctggtccac
     2341 gagcgcttgc acttccacga gcaacggccg cgtgccctcg cgcgtgacca tcaccgcgct
     2401 gcccggcgtc ggcccggcgt gcgcagacag gaagatcgct gacggattcg gcacctcgcg
     2461 caggcccttg tcgcccatcg cgaacacgcc gagttcgttg accgcgccga aacggttctt
     2521 gaacgcacgc agcacgcgga agcgcgagcc ggattcgcct tcgaagtaca gaaccgcatc
     2581 gaccatgtgt tcgag
//
LOCUS       DASRVO010000560         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_249958, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000560 DASRVO010000000
VERSION     DASRVO010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249958"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFF05_06930"
     CDS             complement(<1..232)
                     /locus_tag="VFF05_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZW51575.1"
                     /translation="MSLKAKLTDDMKTAMKGGEKDRLATIRLINAAIKQREVDERIVL
                     DDAQVLAVLEKMLKQRRDSITQFEAANRTDLAD"
     gene            complement(313..528)
                     /gene="rpsU"
                     /locus_tag="VFF05_06935"
     CDS             complement(313..528)
                     /gene="rpsU"
                     /locus_tag="VFF05_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZW51576.1"
                     /translation="MPNVKVRENEPFEFALRRFKRTCEKAGVLAETRKREFYEKPTQE
                     RKRKAAAAVKRHLRRVSRDVTKRKRLY"
     gene            633..1649
                     /gene="tsaD"
                     /locus_tag="VFF05_06940"
     CDS             633..1649
                     /gene="tsaD"
                     /locus_tag="VFF05_06940"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZW51577.1"
                     /translation="MLGIETSCDETGVAVYDVERGLLAHTLYSQIKMHADYGGVVPEL
                     ASRDHVRKLTPLVRETLSRAGMTTRDIAGVAYTAGPGLVGALLVGAASARALAWALDV
                     PAIGVHHMEGHLLAPLLEADPPPPPFVALLVSGGHSMLIEVSAIGHYRLLGDTLDDAA
                     GEAFDKTAKLMGLPYPGGPALAKLAESGAPGKFKFSRPMTDRPGLDFSFSGLKTQVLL
                     AYQQHAGAPDAKADIARAFEDAIVETLLIKCRRALQQTGATRLVIAGGVGANRKLRAQ
                     LADAAQREGFRVYFPRLEFCTDNGAMIALAGALRLRVGLHDDAAIKVAPRWDLASLPG
                     VEAA"
     gene            1649..2011
                     /gene="folB"
                     /locus_tag="VFF05_06945"
     CDS             1649..2011
                     /gene="folB"
                     /locus_tag="VFF05_06945"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZW51578.1"
                     /translation="MDVVFIEDLRIDTVIGIYDWERKIRQTITLDLEMAFDNRKPAAS
                     DKIEDTLDYKKVAKRLIAFVEESHFELVETLAERCADIVRDEFGVAWLRLKLSKPGAV
                     TGSKAVGVAIERGTKPAP"
     gene            2008..>2358
                     /gene="folK"
                     /locus_tag="VFF05_06950"
     CDS             2008..>2358
                     /gene="folK"
                     /locus_tag="VFF05_06950"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZW51579.1"
                     /translation="MTRVWLSLGSNLEPGKNLRAALIELRARFGALIVSPVYRFPAVG
                     FDGPDFLNLAVGIDTDLSAMVLNEWLHALEDRHGRRRNVPRFSSRTLDVDIVLFGDQV
                     LKGPGHLEIPRPELK"
BASE COUNT          396 a          737 c          772 g          453 t
ORIGIN      
        1 tgtccgcaag atcggtgcga ttggcggctt cgaactgcgt gatcgaatcg cggcgctgct
       61 tgagcatctt ctcgagcacg gccagcacct gcgcgtcgtc gagcacgatg cgctcatcca
      121 cctcgcgctg cttgatcgcg gcgttgatca ggcggatcgt ggcgaggcga tccttttcgc
      181 cgcccttcat ggcggtcttc atgtcgtcgg tgagtttggc tttcaggctc atggctttct
      241 ccaaacacta caaatgcaaa cggccggtca cgctcgcgcg taccggccgt cgaaacatcg
      301 caacgcgcag tctcagtaca aacgcttgcg cttggttacg tcgcgcgaca cgcggcgcag
      361 atggcgcttc accgctgcgg cagccttgcg cttgcgttcc tgcgtcggct tttcgtagaa
      421 ctcgcgcttg cgcgtttcgg ccagcacgcc ggccttctcg caggtgcgct tgaagcggcg
      481 cagagcgaac tcgaaaggct cgttctcgcg aactttgacg ttgggcatgg ggtctccgct
      541 gtacactaaa tacccggatg accgggcgag ccgcgtattt taccgtgcaa aaccagcaaa
      601 ttgcaaaatc tgccgacaag ctgaccgggc cggtgctcgg catcgagact tcctgcgacg
      661 aaaccggcgt ggcggtctat gacgtcgagc gcggtctgct ggcgcacacc ctgtacagcc
      721 agatcaagat gcacgccgat tacggtggcg tggtgccgga actggccagc cgcgaccatg
      781 tgcgcaagct cacgccgctg gtccgcgaga cgctttccag ggccggcatg accacgcgcg
      841 acatcgccgg cgtggcctat acggccgggc ccggcttggt cggcgccttg ttggtgggtg
      901 ccgccagcgc acgcgcgctg gcgtgggcgc tggatgtccc ggccatcggc gtgcatcaca
      961 tggaagggca tctgctcgcg ccgctgctcg aggccgatcc gccgccgccg ccgttcgtgg
     1021 ccctgctggt gtcgggcggg cattccatgt tgatcgaggt ttctgccatc ggccactatc
     1081 gcctgctcgg cgacactctg gacgatgctg ccggcgaggc tttcgacaag accgccaaac
     1141 tgatgggact gccctatccg ggcggcccgg cgctggcgaa actggccgaa tccggcgcgc
     1201 caggcaagtt caagttctcg cgaccgatga cggatcgccc agggctggat ttcagttttt
     1261 cgggactcaa gacgcaggtg ctgcttgcgt accagcagca cgccggcgcg ccggatgcga
     1321 aagccgacat cgcgcgtgcc ttcgaggacg ccatcgtcga aaccctgttg atcaagtgtc
     1381 ggcgcgcgct gcagcagacc ggcgcgacgc ggctggtgat cgccggcggt gtcggcgcga
     1441 accgcaagct gcgcgcgcag cttgccgatg cggcgcagcg cgaaggattt cgcgtgtatt
     1501 tcccgcgcct tgaattctgc accgacaatg gcgcgatgat cgcgctggcc ggcgcgctgc
     1561 gcctgcgggt cgggctgcac gacgatgccg cgatcaaggt ggcgccgcgc tgggacctgg
     1621 cctcgctgcc cggcgtggag gctgcgtaat ggacgtcgtc ttcatcgaag acctgcgcat
     1681 cgacaccgtc atcggcattt acgactggga gcgcaagatc cgtcagacca tcacgctcga
     1741 tctggaaatg gccttcgaca accgcaagcc ggcagcgagc gacaagatcg aagacacgct
     1801 ggattacaag aaagtcgcca agcgcctgat tgcgttcgtg gaagaatcgc atttcgagct
     1861 cgtggagacc ttggccgaac gttgtgcgga tatcgtgcgc gatgaattcg gcgtggcgtg
     1921 gctgcgattg aagttgtcca agcccggcgc ggtgacgggg tcgaaggcgg ttggggttgc
     1981 gatcgaacgc ggcacgaagc cggcgccgtg acgcgcgtct ggctcagtct cggctccaat
     2041 ctcgaaccgg gaaaaaacct gcgcgcagcg ctcatagagc tgcgtgcacg cttcggcgcg
     2101 ctgatcgtgt cgccggtcta tcgcttcccg gcggttggct tcgacggtcc tgatttcctc
     2161 aatctcgccg tagggatcga caccgacttg agcgccatgg tgctcaacga gtggctgcat
     2221 gcgctggaag atcgtcacgg ccgccgccgc aacgtgccac gattttccag tcgcacgctc
     2281 gatgtcgaca ttgtgttgtt cggcgatcag gtgctgaaag ggccgggtca tctggagatt
     2341 ccgcgcccgg aattgaaa
//
LOCUS       DASRVO010000561         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_328404, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000561 DASRVO010000000
VERSION     DASRVO010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328404"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..414)
                     /locus_tag="VFF05_06955"
     CDS             complement(43..414)
                     /locus_tag="VFF05_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP-related RiPP peptide"
                     /protein_id="HZW51580.1"
                     /translation="MQRLPSAEYRRLGRRPASITNVSTGGDMAGPNFSKAQMLDLLGR
                     LASDDGFRANFEKNPASALSQIGLPADQVKVFLTDHIVAGKLATKGEFDGALKRIANQ
                     NVEECLCMTIPSLKLDFRAYK"
     gene            complement(447..764)
                     /locus_tag="VFF05_06960"
     CDS             complement(447..764)
                     /locus_tag="VFF05_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP-related RiPP peptide"
                     /protein_id="HZW51581.1"
                     /translation="MAYANHVSKSQIIELLGKLAEDDGYRIRFQRDPDAALTELDFPV
                     GGFAGFVGTSAQSGALADKSVFAIARKRMVDEIAGECLCMIIPNFRLDYGDQHRRSAT
                     ALQ"
     gene            926..2101
                     /locus_tag="VFF05_06965"
     CDS             926..2101
                     /locus_tag="VFF05_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptide maturation dehydrogenase"
                     /protein_id="HZW51582.1"
                     /translation="MQVRRCVHLMIEPRERLDFDLSLLANGGTGLRNVVEWIALAPHR
                     DEEIVLSADEMAVLGALSPSQWTALDELAKMHPRATLQALLDKGLLIAQADGDAAARE
                     ADEFLRDTHWRGLDAVAHRFSRWRSVDTVEAQRRFGEETDQTFLERLGSSPAPVRERV
                     EPSRRRKLPAAGDSPLDRLFARRVTCRNYDLARQLDLAQFGAAMYRAFGARAVDEYAP
                     GIFLLKKGAPSAGGLHPVEAYVLVQRVDGIVPGLYHYHPAAHALEPIREMDATELEKL
                     AKTCLGGQPYFAEAHVVVALAGRFARNFWKYRNHAKAYRAVILDAGHLSQAMYLAATE
                     LNLGAFITAAINEVEIEQAFDLEPMAEGPLAVCGFGMRAQERVTVEFDPAHKVWDHA"
BASE COUNT          384 a          680 c          669 g          372 t
ORIGIN      
        1 tgagcgacgg caggcgctgc agcactagcc gtaaatccaa tattatttgt aggcgcggaa
       61 gtccagtttc agacttggga tcgtcataca aaggcattct tccacgttct gattcgcgat
      121 gcgtttgagc gcaccgtcaa attccccctt cgtcgccaac ttgccagcaa cgatgtggtc
      181 ggtgagaaaa actttgacct gatcggccgg caagcctatt tgcgacagag ccgacgccgg
      241 gtttttctcg aagttcgcac gaaagccgtc atcgctcgcg agacggccaa gcagatcgag
      301 catctgggcc ttgctgaaat ttggaccagc catatctccc cctgtcgaga cgtttgtgat
      361 ggacgcaggc cgcctgccga gccgtcgata ttcggccgac ggcaggcgct gcaccgggac
      421 ggcaatagcc gatcccggcg gtcatattac tgcaacgcgg tggcgctgcg acggtgctga
      481 tcgccatagt ccagccgaaa attcggaatg atcatgcaca ggcattcgcc ggcgatttcg
      541 tcgaccatgc gcttgcgcgc gatcgcaaaa acggatttgt ccgccaacgc accgctttgt
      601 gccgaagtgc cgacgaatcc ggcaaagccg ccgacgggga aatccagttc cgtcagtgcg
      661 gcatccggat cgcgctgaaa gcggatgcgg tagccgtcgt cctcggcgag tttgcccaac
      721 agttcgatga tctgcgattt gctgacgtga tttgcgtaag ccatgacttg ctccgaaaga
      781 gtgaccccct gagtgctcga ccctagcagc gcctgcaaaa ggctcagtga cagtgatcac
      841 aaatccggaa cccggcgcac gcgcggcttg ccagacgcac gcacgctgcc tacgcttgcg
      901 cgcacttccc cgcgagtgat ccgccatgca agtacgccgc tgcgtccacc tgatgatcga
      961 accgcgcgag cggctggact tcgatttgtc attgctcgcc aatggcggca ccggcctgcg
     1021 caacgtggtc gagtggatcg cgctggcgcc gcaccgcgac gaggaaatcg tgctgtcggc
     1081 ggacgagatg gccgtgctcg gcgcgctgtc gccgtcgcag tggacggcgc tggacgaact
     1141 cgcgaaaatg catccgcgcg cgacgttgca ggcgttgctg gacaagggcc tgctgatcgc
     1201 tcaagcggac ggtgacgccg ctgcgcgcga ggccgacgaa ttcctgcgcg atacgcactg
     1261 gcgcggactc gatgccgtcg cgcaccgctt ctcgcgctgg cgcagcgtgg ataccgtgga
     1321 ggcgcagcgg cgcttcggcg aggaaaccga ccagaccttt ctcgaacgcc tcggcagctc
     1381 gcccgcgccg gtgcgcgaac gcgtcgaacc gtcgcggcgg cgcaagcttc cggcggccgg
     1441 cgatagtccg ctcgacaggc tgttcgcgcg gcgcgtcacc tgccgcaatt acgacctggc
     1501 gcgccagctc gacctcgccc agttcggcgc ggcgatgtac cgcgcgttcg gcgcgcgcgc
     1561 ggtggacgaa tacgcacccg gcatcttcct gctcaagaag ggcgcgccat cggcgggcgg
     1621 cctgcatccg gtcgaggcct acgtgctggt gcagcgcgtg gatggcatcg tgccgggcct
     1681 gtaccactac catccggccg cgcatgcgct cgagccaatc cgcgagatgg acgccacgga
     1741 gctggagaaa ctggccaaga cctgcctcgg tggacaaccg tatttcgccg aagcgcatgt
     1801 ggtggtcgcg ctggccggac gcttcgcgcg caatttctgg aaataccgca accacgccaa
     1861 ggcctatcgc gcggtgatcc tggatgccgg gcacctgtcg caggccatgt acctcgccgc
     1921 caccgagttg aacctcggcg cgttcatcac cgccgcgatc aacgaagtcg agatcgagca
     1981 ggctttcgat ctcgaaccga tggccgaagg cccgctggcc gtttgtggct tcggcatgcg
     2041 cgcgcaggaa cgcgtgaccg tcgagttcga tccggcgcac aaggtgtggg accacgcgtg
     2101 accgc
//
LOCUS       DASRVO010000562         1123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_171130, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000562 DASRVO010000000
VERSION     DASRVO010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171130"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..>1123
                     /gene="kbl"
                     /locus_tag="VFF05_06970"
     CDS             75..>1123
                     /gene="kbl"
                     /locus_tag="VFF05_06970"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HZW51583.1"
                     /translation="MSYSAKARYASELDAIREQGLFKAERIIVSPQSAEIKLEGGRKV
                     LNFCANNYLGLADHPAVIQAAKDALDSHGFGMASVRFICGTQDLHKQLEAAIAKFFGS
                     DDAILYTSCFDANGGLFETILGEEDVVISDALNHASIIDGIRLCKAERRRYANCDMAE
                     LEKHLQETQGRRTRLIATDGVFSMDGTIAKLDAIVALKKKYNALLMVDDSHATGFVGP
                     SGRGSAELHGVMDEVDVFTSTLGKALGGGSGGFTTGRREIIDLLRQRSRPYLFSNTLP
                     PPLVAASIKVFEMLSSSSALRDTVNANALYFREQMTKASFDIKPGTHPIVPVMLYDAK
                     LAQQFAADLLDEGIYV"
BASE COUNT          221 a          385 c          331 g          186 t
ORIGIN      
        1 cggcgccgtg gcttccttcc cagcacggcg ccaaacggtc aaaatagcgc tttgcccacc
       61 ggccggaatc ccccatgagc tactcagcca aggcgcgcta cgccagcgaa ctcgacgcca
      121 tccgcgaaca gggcctgttc aaggccgagc gcatcatcgt ctcgccgcag tccgccgaga
      181 tcaagctgga aggcggccgc aaggtgctga acttctgcgc caacaactac ctcggcctgg
      241 ccgatcaccc cgcggtgatc caggccgcca aggatgcgct ggacagccac ggcttcggca
      301 tggccagcgt gcgcttcatc tgcggcacgc aggacctgca caagcagctc gaggccgcga
      361 tcgcgaagtt cttcggcagc gacgatgcga ttttgtatac cagttgcttc gacgccaacg
      421 gcggcctgtt cgaaaccatc ctcggcgaag aggacgtggt catttccgat gcgctcaacc
      481 acgcttccat catcgacggc atccgcctgt gcaaggccga gcgccggcgc tacgccaact
      541 gcgacatggc ggaactcgaa aagcacttgc aggaaaccca aggcaggcgc acgcgcctga
      601 ttgccaccga cggcgtgttt tcgatggacg gcaccatcgc caaactcgat gctatcgtcg
      661 cgctgaagaa aaaatacaac gcgctgctga tggtcgacga ttcgcacgcg accggcttcg
      721 tcggtcccag cggacgcggc tcggcggaac tgcacggcgt gatggacgag gtcgacgtgt
      781 tcacgtccac gctcggcaag gcgctcggcg gtggctccgg cggattcacc accggacggc
      841 gcgagatcat cgacctgctg cgccagcgct cgcggccgta cctgttttcg aacacgttgc
      901 cgccgccgct ggtcgccgcc tcgatcaagg tgttcgagat gctctcgtcg tccagcgcac
      961 tgcgcgacac cgtcaatgct aacgcgctgt acttccgcga acagatgacg aaggccagct
     1021 tcgacatcaa acccggcacg catccgatcg tgccggtgat gctctatgac gccaagctcg
     1081 cgcagcaatt cgccgcggac ctgctcgacg aaggcatcta cgt
//
LOCUS       DASRVO010000563         1039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_93747, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000563 DASRVO010000000
VERSION     DASRVO010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93747"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>1039
                     /locus_tag="VFF05_06975"
     CDS             116..>1039
                     /locus_tag="VFF05_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HZW51584.1"
                     /translation="MHGLISSGGRQAVELIAVNADSLEKTARRVGGNARDWVAANGFC
                     GDPGTFCALPDAKGQRRAVFVGVANDDDVYALSNLPYSLAAGDYRLATGIGVALDPLR
                     AALGWGMGAYRFARYRKSMRDAANLAVDAGTLRAVSPLLDGIACVRDLVNTPTQDMGP
                     AELTAEVAALARRHRAKFHEWAGVALLKANFPTIHAVGRGSHRLPRLAELTWGRANDP
                     HLVLVGKGVCFDTGGLDLKTADGMRWMKKDMGGAAHAIALAGLIMQARLPVRLTLLIP
                     AVENAISGASFRPRDIYTSRKGITVEIGNTDA"
BASE COUNT          182 a          311 c          351 g          195 t
ORIGIN      
        1 ccgacggcgg aaaccgccga gtcacaagtg ttggcatagt ccctgcttta ccggatgtaa
       61 cgtcgcaact catctgccta acgcgttgaa acgacgacat ctcgaaggaa taacgatgca
      121 cggactgatt tcctccggcg gccgccaagc cgtcgagttg atagcggtga atgcggacag
      181 cctggaaaag accgcacgcc gggttggcgg caatgcgcgt gattgggtcg ccgcaaatgg
      241 tttttgtggc gatcccggca cgttttgcgc gttgcctgac gcgaaagggc agcgccgcgc
      301 cgttttcgtg ggtgtggcga atgacgatga cgtttacgcc ttgtccaact tgccgtacag
      361 cctggcggct ggagactacc ggctcgcgac cggtattggc gttgctctgg atccgttgcg
      421 cgcggccctt ggctggggca tgggtgccta tcgttttgcg cgctatcgca agtcgatgcg
      481 tgatgcggcc aatttggcgg tagatgctgg cacgttgcgc gctgtttcgc cattgctcga
      541 tggcatcgca tgcgtgcgcg acctggtcaa cacgccgacc caggacatgg gtccggcgga
      601 actgacggcg gaagtggccg cgctagcgcg ccggcaccgc gccaaattcc acgaatgggc
      661 gggcgtggca ttgctgaagg cgaacttccc gaccatccac gcggttggtc gcggcagcca
      721 tcggctacct cggctggccg agctgacctg gggccgggcg aacgatccgc acctcgtgct
      781 ggtcggcaag ggcgtgtgtt tcgacaccgg cggcctggac ctgaaaaccg ccgacggcat
      841 gcgctggatg aagaaggaca tgggcggcgc ggcgcatgcg atcgcgctcg ccggcctgat
      901 catgcaggcg agattgccgg tacgcctgac cttgctgatc ccggcggtgg agaatgcgat
      961 ctccggcgcg agtttccgcc cgcgcgacat ctacacatcg cgcaaaggca tcacggtcga
     1021 gatcggcaat accgacgcg
//
LOCUS       DASRVO010000564         1556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_302998, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000564 DASRVO010000000
VERSION     DASRVO010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302998"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFF05_06980"
     CDS             <1..354
                     /locus_tag="VFF05_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HZW51585.1"
                     /translation="HPPHDGADLFERCKGLRALRGPQREELLDVLHAPLTAEELDFAP
                     IPPPPTPADRRLLSALRETVTGIAQSLNLPEGLLCPRRHVEALIAERTWPAALEGWRK
                     PLLHDALMARIGSGA"
     gene            623..1556
                     /locus_tag="VFF05_06985"
                     /pseudo
     CDS             623..1556
                     /locus_tag="VFF05_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
BASE COUNT          256 a          567 c          488 g          245 t
ORIGIN      
        1 catccaccgc acgacggcgc cgacctgttc gagcgctgca agggcctgcg cgccctgcgc
       61 ggtccgcagc gcgaggaatt gctggacgtg ctgcacgcgc cgctgacagc ggaggaactc
      121 gacttcgcgc cgattccgcc gccgccaacg ccggcagacc ggcgcctgct cagcgcgctt
      181 cgcgaaaccg taaccggcat cgcgcaatcc ctgaacctgc ccgaaggcct gctctgtccg
      241 cgccggcacg tggaagcgct gatcgccgaa cgcacctggc cggcggcgct cgaaggctgg
      301 cgcaaaccgc tgctgcacga tgcgctgatg gcccgcatcg ggtccggcgc ttgacaagcc
      361 cacgcggcat gcgttccact cacgccatgc gcaaggcgtt ctccaccacg acgaccacta
      421 ccaccacgac taacgtggtg cggttggccg tgcgcagata gttttccccc aagcaacggc
      481 cccgcaccct cgacaggatg cggacgcccg gtgcgatcca tccgacgacg atgcggggcc
      541 tcgaaccgga ggttccatga acgctgtcgc ccgcgatgtc ctgtccaagc cgtgcgtggc
      601 aatccgcgcc aggcccgcga cgacgctcgc cctgggcctg atcgggcccg gcaaggtcgg
      661 ctcggccctg ctcgaccaga tcgccgccgc cgcaccgcgc ctgcggcgtg cgggcgcgct
      721 ggacctgcgc ctgcacgcga tcgtgtcgac aagattcctg ctcgcgcatg cgcacgacct
      781 cggcgcgaac tggcgcgatc gcgacgccga tatcgtcgac aacgacatcg acggattcct
      841 gcgccgtctg cttgccgcgc gctcgccgca cgctgtgatc ctggattgtt ccggcagcga
      901 tgcgatcgcc gcgcgctatc cgcaatggct cgccgccggc atccacgtga tcactccgaa
      961 caagcaggcc ggcgccggcc cgatggaaaa gtatcgagcg atccgcgacg ccgccgcgcg
     1021 cggcgatgcg cgttttcgct acgaggcgac ggtcggtgcc ggcttgccgg tgatatcgac
     1081 cttgcgtgac ctgctcgaca ccggcgacga attgcatgcg gccgagggaa ttttctccgg
     1141 cacgctggcg tggctgttca accgtttcga tggcacgcaa tcgttttcgg cgctgatccg
     1201 cgaagcgcat gcgctcggct acaccgaacc cgatccgcgc gacgacttgt ccggcaccga
     1261 tgtcgcgcgc aaactcgtca tcctcgcgcg cgaagcgggc cgcgacttgt ccttgtccga
     1321 cgtggatgtc gaaagcctgg ttccaacatc attgcgcggc gccagcgccg gcgaattcat
     1381 ggcccgcctt gatcaactcg atgccgacat gaccaaacga ttcgcggctg cgcaagccac
     1441 gggcaaggtc ctgcgccacg tcgcgcgact gggtcgcgac ggtagcgcga gcgttggcct
     1501 ggtcgaactc gaacgtgaac acgccttcgc caatatccgc ctgaccgaca acatcg
//
LOCUS       DASRVO010000565         1148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_250238, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000565 DASRVO010000000
VERSION     DASRVO010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250238"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1148)
                     /locus_tag="VFF05_06990"
     CDS             complement(<1..>1148)
                     /locus_tag="VFF05_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HZW51586.1"
                     /translation="YKKMLAEHKNFTQVMDVYGFRVVAAATMDCYRALGAIHALYKPL
                     DGRFRDFIAIPKANGYQSLHTVLFGPFGAPIEIQIRTEEMDQVAERGVAAHWVYKTSS
                     EPATAAQARAREWVAGLVDEQTREASSVEFLENVKVDLFPDEVYLFTPKGRILALPRN
                     ATALDFAFAVHTDVGSHAVAARVDGTLAPLRQRLNSGETVEIITAPSAAPTPQWLEWV
                     ASAKARTAIRHFLKHLQHEDAVDFGHRMLDRALDARGTSLDAISGDALDRFLVDARYK
                     RLEELLSDIALGNRMPDQVAAQLVQALGGAGELLPHEAHAQEKILISGAERGVLSFGN
                     CCNPVPGDEIAGYLSAGKGVVVHRTDCPNMPEYRKTPERIVAIDWDRE"
BASE COUNT          186 a          367 c          392 g          203 t
ORIGIN      
        1 cttcgcggtc ccagtcgatg gcgacgatgc gctcgggcgt cttgcggtat tcgggcatgt
       61 tcgggcaatc ggtgcggtgc acgacgacgc cctttccagc cgaaaggtaa ccggcgatct
      121 cgtcgccggg caccggattg cagcagttgc cgaagctgag cacgccgcgt tcggcgccgc
      181 tgatcaggat tttttcctga gcatgagcct cgtgcggcag cagttctccg gcgccgccaa
      241 gcgcctgcac gagttgcgcc gcgacctgat cgggcatgcg gttgccgagc gcgatgtcgg
      301 agagcaattc ctccaggcgc ttgtagcgcg cgtccacgag aaatcgatcc agcgcatcgc
      361 cggaaatcgc gtcgagactg gtgccgcggg cgtccagcgc gcgatcaagc atgcggtggc
      421 cgaagtcgac ggcatcctcg tgctgcaggt gcttgaggaa atgccggatc gcggtgcgcg
      481 ccttggccga ggcgacccat tccagccatt gcggcgtcgg cgcagccgag ggcgcggtga
      541 tgatctcgac ggtttcgccg gaattcaggc gctggcgcag cggtgcgagc gtcccgtcca
      601 cgcgcgcggc gacggcatgg ctgccgacgt cggtgtgcac ggcgaaagcg aaatcgagcg
      661 ccgtagcgtt gcgcggcagg gcgaggatgc ggcccttcgg cgtgaacagg tagacctcgt
      721 ccggaaacaa atccactttg acattttcga gaaactcgac cgacgaggct tcgcgcgttt
      781 gctcgtcgac cagccccgcc acccactcgc gcgcgcgcgc ctgcgcggcc gtggccggct
      841 ccgaggacgt cttgtagacc caatgcgcgg cgacgccgcg ctcggcgacc tgatccattt
      901 cctcggtgcg gatctggatt tcgatcggcg cgccgaacgg gccgaacagc accgtgtgca
      961 gcgactggta gccgttcgcc ttgggaatgg cgatgaagtc gcggaagcgg ccgtccagtg
     1021 gcttgtacag cgcatggatc gcgccgagcg cccgatagca atccatcgtc gccgctgcca
     1081 cgacgcggaa tccgtacacg tccatgacct gggtgaagtt cttgtgctcg gccagcatct
     1141 tcttgtag
//
LOCUS       DASRVO010000566         1833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_328560, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000566 DASRVO010000000
VERSION     DASRVO010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328560"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..597
                     /gene="trxC"
                     /locus_tag="VFF05_06995"
     CDS             160..597
                     /gene="trxC"
                     /locus_tag="VFF05_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010365869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HZW51587.1"
                     /translation="MGESLHIVCPHCAAVNRVPSDRLGAAPTCGQCHRPLFSGHPLET
                     GEAAFEKHLARNDIPVLVDFWAPWCGPCRMMAPQFVQAAAQLEPRVRLLKVDTDAEPA
                     LGTRYNIRSIPTLALFRGGREVARQAGATNSAGIVQWVRANGA"
     gene            759..>1833
                     /locus_tag="VFF05_07000"
     CDS             759..>1833
                     /locus_tag="VFF05_07000"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZW51588.1"
                     /translation="MSLRGTFGSLALFVVAASSVSAATITVTNINDSGAGSLRAALAA
                     AAAGDTIAFSAAATGTIPLASTLTVALPVTIQGPGAAALTISGQGTIRVFDVVAGGGL
                     TLSGVTIANGSTTGTGAAIQNAATVAVARSVITGNTAGNGGIANLAGATITVDETTFD
                     TNTATSVGGGALINFGTATLTNSTLSNNAAPINGAAINTQPAGVTTVINCTFVGNVSA
                     SIGGATSNLGTTTILDSTFSANQATSGSGIATGNSNVTLNNDIFADGSSDAINPVGSV
                     TGSNDVFFNNNSASDATGYSTTNFIAATSEPVGALAQNGGPTQTMLPLPGGGAVCNGS
                     IALLPAGLTRDQRGFPRVVDACLD"
BASE COUNT          334 a          636 c          559 g          304 t
ORIGIN      
        1 caaggccgaa aagcctgaca aaagtaatga tgtggccgga cggaaagcct gggacagcct
       61 tgcctggtat caggtccctg tctcgggcac cgctgcccgc gttgccccgg caggtttcgt
      121 ccccatatcg ggcggcaggc aaccgaccga gtccacgcca tgggcgaatc cctgcatatc
      181 gtctgccccc actgcgcggc ggttaaccgc gttccttcgg atcgcctcgg cgctgcgccg
      241 acctgcggcc aatgccaccg cccactgttc tccggccatc cgctggaaac cggcgaagcc
      301 gcgttcgaga aacatctcgc gcgcaacgac attccggtgc tggtggattt ctgggcgccg
      361 tggtgtggcc cgtgccggat gatggcgccg cagttcgtgc aggcggcggc gcagctggaa
      421 ccgcgcgtgc gcctgctgaa ggtcgacacc gacgccgaac ctgcgctcgg tacgcgctac
      481 aacatccgca gcatccctac ccttgcactg tttcgcggcg gacgcgaagt ggcgcggcag
      541 gccggcgcga cgaacagtgc cggcatcgtg caatgggtac gcgcgaacgg tgcgtgaggc
      601 ggaatccaac ccaacgcttt gacatgcggg cccgatatgg cgcaaatatc tctgctcggc
      661 aaagcgtata ctcaagcgcg ggtcgctcct gattcctgcg gttcgttcag gatcgcaata
      721 cgatcgcact cacgacttcg caaccgtagg ggattcgcat gtcgctacgt ggcacgtttg
      781 gaagcctcgc gctgttcgtc gtcgcggcat cgtccgtatc ggccgcgacg atcaccgtca
      841 ccaacatcaa cgatagcggc gctgggtcct tgcgcgcggc gctcgctgcg gccgcagccg
      901 gcgacaccat cgcattctct gcagccgcga ccggaaccat cccgctggct tcgacgctca
      961 ctgtcgcact gccggtgaca atccagggtc cgggcgccgc cgcgctgacg atcagcggcc
     1021 aaggcacaat ccgggtattc gacgttgtcg ccggcggcgg tctgacgctc tccggcgtca
     1081 cgatcgccaa tggctcgacc accggaaccg gcgctgccat ccagaatgcc gccacggttg
     1141 cggtggcacg aagcgtcatt accgggaaca cggcgggaaa tggcggcatc gcaaatctgg
     1201 ccggcgccac aataaccgtc gacgagacca cattcgacac caacacggcg accagcgtcg
     1261 gcggcggagc gctcatcaat ttcggcaccg cgacgctgac caacagcacg ctctcgaaca
     1321 acgccgcccc gatcaacggc gctgccatca acacgcagcc ggcgggcgtc accaccgtga
     1381 tcaactgcac gttcgtcggc aatgtctccg ccagcatcgg cggggccacg tcgaatctcg
     1441 gcacgacgac gattctggac agcacgttct ccgccaacca ggcgacttcg ggttccggca
     1501 tcgccaccgg caattccaac gtgacgctca acaacgacat cttcgccgac ggttccagcg
     1561 acgcgatcaa tccggtcggc tcggtaaccg gcagcaacga cgtgttcttc aacaacaact
     1621 cggcctcgga cgcgaccggt tacagcacca ccaatttcat cgccgcgacg tccgaaccgg
     1681 tcggtgccct tgcccagaac ggtggtccaa cgcagaccat gctcccgctg ccgggcggcg
     1741 gtgcggtttg caacggatcg atcgcgctgc ttccggcagg gttgacgcgc gatcagcgcg
     1801 gtttcccgcg cgtggtggac gcgtgtctgg atg
//
LOCUS       DASRVO010000567         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_328578, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000567 DASRVO010000000
VERSION     DASRVO010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328578"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1156
                     /locus_tag="VFF05_07005"
     CDS             <1..>1156
                     /locus_tag="VFF05_07005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZW51589.1"
                     /translation="TAFDLAIVDYFMPGDPGTVLVQRLKNDARTANVLAAVITGTYSD
                     RVINESLAAGALECLFKSEARELFLARLGSLARAVQDRKSVDAERRRLQSILSSVGDG
                     VYGVDGRGTIQFVNPAALDMLGYEAAADLVGESAYDSFHHSYEDGTAVPRSACFLSQC
                     YAQGSQVPGWQTVFWSRAKRPVPVECTVYPMQIEGHREGSVVAFRDISARRMLEEELR
                     WQASHDALTKLYNRAWFETQLEQEIARLKRTDQVSLLLFLDLDRFKYINDTAGHTAGD
                     QLLIEIGRRLSARLRASDHIARMGGDEYAVILRNVINADVAQISDEFRKALGALPFVY
                     GGKSYRVGTSIGVARLDQHTPSQSEAMAAADVALHLAKRNGRNQIHVFSAD"
BASE COUNT          211 a          396 c          352 g          197 t
ORIGIN      
        1 accgcgttcg acctcgccat cgtcgactat ttcatgcccg gcgatccggg cacggtgctg
       61 gtgcagcggc tgaagaacga tgcgcgcacg gcgaacgtgc tcgccgccgt catcaccggc
      121 acctattccg atcgcgtcat caacgaatcg ctggccgccg gcgcactgga atgcctgttc
      181 aagagcgagg cgcgggaatt gttcctcgcc cgcctgggct cgcttgctcg cgccgtgcag
      241 gatcgcaagt cggtcgatgc cgaacgccgc cgcctgcaaa gcatcctcag ttccgtcggc
      301 gacggcgtgt acggcgtgga cggccgcggc acgatccagt tcgtcaatcc cgccgcgctc
      361 gacatgctcg gctacgaagc ggccgccgac ctggtcggcg aatcggccta cgacagtttc
      421 catcacagct acgaagacgg caccgcggta ccccgcagcg cctgcttcct ctcgcagtgc
      481 tacgcgcagg gcagccaggt tcccggctgg caaacagtgt tctggagtcg cgccaaacgt
      541 ccggtgccgg tggaatgcac ggtctatccg atgcagatcg agggccaccg cgaaggatcg
      601 gtcgtggcat tccgcgacat ttccgcgcgc cgcatgctgg aagaagaact gcgctggcag
      661 gccagccacg atgcgctgac caagctctac aatcgcgcct ggttcgaaac ccagctcgaa
      721 caggaaatcg cgcgcctgaa gcgcaccgat caggtatcgc tgctgctgtt cctcgatctg
      781 gatcgtttca aatacatcaa cgacaccgcc ggtcacaccg ccggcgacca gttgctcatc
      841 gaaatcggca ggcgcctgtc cgcgcgcctg cgcgccagcg accacatcgc acgcatgggc
      901 ggcgacgagt atgcggtgat cctgcgcaac gtcatcaacg ccgatgtcgc gcagatctcc
      961 gacgaattcc gcaaggcgct cggtgccctg cccttcgtct acggcggcaa gagttatcgt
     1021 gtcggcactt cgatcggcgt ggcgcggctc gatcagcaca cgccgtcgca aagcgaggcg
     1081 atggcggccg cggacgtagc cctgcacctg gccaagcgca atggtcgcaa ccagatccac
     1141 gtgttttctg ccgata
//
LOCUS       DASRVO010000568         1162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_93960, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000568 DASRVO010000000
VERSION     DASRVO010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93960"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFF05_07010"
     CDS             complement(<1..921)
                     /locus_tag="VFF05_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51590.1"
                     /translation="MAAGLNHVIATVNTAIGIYDKQGNLLGGGPVESDTFFNSANCTG
                     TFDPTVEYDEVADHFIINYDASPNDCIAVSQTGDPTGAWNVYSFNSGIDAGGLFDYPH
                     IGVGDQAIYVGANMFFNSSPFAGRVWALNKAQMYAGQALTLPTPHDLVDGGNADGTPT
                     PMVLHGAPSAPGTVYIITDDTNFSGDTFGVWQWTDSTGVAAPTLVGYADLATATGVTA
                     NFPIDQTQLGTPVAIQANDVRTLDAEWRNGHLWLTHQMSCNIGAGAQGCARWAEVDPS
                     DASVVQAGVVSIFGKSISFPNLAVDANDNMA"
BASE COUNT          195 a          368 c          366 g          233 t
ORIGIN      
        1 cgccatgttg tcgtttgcat ccaccgccag gttcgggaat gaaatggact tgccgaaaat
       61 cgacaccacg ccggcctgga cgacgctggc gtcgctcgga tccacttccg cccagcgcgc
      121 gcagccttgc gcgccggcgc cgatgttgca cgacatctgg tgcgtgagcc acaaatggcc
      181 gttacgccat tccgcatcga gcgtgcgcac gtcgttggcc tgaatcgcga ccggcgtacc
      241 aagctgcgtc tggtcgatcg ggaaatttgc ggtcacgccg gtggcggtgg ccagatccgc
      301 gtaaccgacc agcgtcggtg cggccacgcc ggtcgaatcg gtccattgcc acacgccgaa
      361 tgtgtcgccg ctgaagttgg tatcgtcggt gattatgtac accgttccgg gtgcggacgg
      421 tgcgccatgc agcaccatcg gcgtgggcgt accgtcggca ttgccgccat cgaccagatc
      481 gtgcggtgtc ggcagcgtga gcgcctgccc ggcgtacatc tgcgccttgt tcagcgccca
      541 cacgcgacct gcaaacgggc tgctgttgaa gaacatgttc gcgccgacat agattgcctg
      601 atcgccgacg ccgatgtgcg gatagtcgaa cagtccgccc gcatcgatgc cggaattgaa
      661 cgaatacacg ttccacgcgc cggtgggatc gccggtctgg ctgaccgcga tgcaatcgtt
      721 cggcgaagcg tcgtagttga tgatgaagtg gtcggcgact tcatcgtact cgaccgtggg
      781 atcgaatgtg cccgtgcaat ttgcggagtt gaaaaacgtg tccgattcca ccgggccgcc
      841 gccgagcagg ttgccctgct tgtcgtagat gccgatcgcc gtgttgacgg tagcgatcac
      901 gtggttcaga cccgcggcca tgtgggtatc cggcggtacc gatgcactgt tgccgcagca
      961 ttggctgata tcgggtcctt caaagccccc aagcagattc accacatccg tcgacggcgt
     1021 gctcggttgg cgccccgcat gcgtgtcttc ctcgagcctc tgcacatgca gcgtacgcac
     1081 cggctgcaca agcgcggctg cgcgccggct gtccgcctcg gtttccgaaa tcggcgaacg
     1141 ttcgcgggtt tcgctttccg ca
//
LOCUS       DASRVO010000569         1970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_43522, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000569 DASRVO010000000
VERSION     DASRVO010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43522"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFF05_07015"
     CDS             complement(<1..527)
                     /locus_tag="VFF05_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZW51591.1"
                     /translation="MSTIGILGGGQLARMLALAGAPLGVRFLAVDSVADACAGQVAPL
                     LVADWRDLAALEKFAARVDVVTFDFENVPADTARWLAERVRVSPNPRALALAQDRLAE
                     KSMFRDLGLATADFATVDSRADLAHAARTLGYPAILKTRRLGYDGKGQFRLRNEADID
                     AAWEAVAAAPSILEA"
     gene            complement(538..1047)
                     /gene="purE"
                     /locus_tag="VFF05_07020"
     CDS             complement(538..1047)
                     /gene="purE"
                     /locus_tag="VFF05_07020"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003468225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZW51592.1"
                     /translation="MNTAKALHPRIGVVMGSRSDWDTLQHAADTLDRLGIAHEVRVVS
                     AHRTPDLLFDYASQARARGLRAIIAGAGGAAHLPGMLAAKTSVPVLGVPVQSQALNGM
                     DSLLSIVQMPAGVPVATFAIGKAGATNAALFAAALLAGEDAGVARRLDEFRAAQTDAV
                     LAKSDPRAS"
     gene            1107..1376
                     /locus_tag="VFF05_07025"
     CDS             1107..1376
                     /locus_tag="VFF05_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HZW51593.1"
                     /translation="MDKRLLDILCCPATKQPLRPLTRAELDALNRVIAGGGVRTTAQA
                     TVDAAFAAGLITVDGKRVYRIDDDIPVMLADEAIMVSQLDGFPAH"
     gene            1519..>1970
                     /locus_tag="VFF05_07030"
     CDS             1519..>1970
                     /locus_tag="VFF05_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZW51594.1"
                     /translation="MNKPDDTSKIVDLQQRQSMQAAGGDRLGDLLRAVRATALKRLHG
                     LVATLFENIDDALFDLAEKAENNAIQTQYFDGMREVRKKRQLFERVYQEQLGRLFNDF
                     ANGKLQANRPDPSKAEGDGLSLVEDSELEESLAIASAVAKNEGRLARPL"
BASE COUNT          357 a          638 c          660 g          315 t
ORIGIN      
        1 caggcttcca ggatcgacgg cgccgcggca accgcctccc aggcggcatc gatgtcggct
       61 tcgttgcgca ggcggaattg gcccttgccg tcgtaaccta accggcgcgt cttcagtatc
      121 gccggatagc cgagagtgcg cgcggcgtgc gcgaggtccg cgcggctgtc gacggtggcg
      181 aaatccgcag tcgccaggcc gaggtcgcgg aacatggatt tttccgcgag acggtcctgg
      241 gccagggcga gtgcgcgcgg attcggcgac acgcgcacgc gttcggcgag ccaacgcgcg
      301 gtatcggcgg ggacgttttc gaaatcgaat gtgaccacgt cgacgcgagc cgcgaatttt
      361 tccagcgccg ccaaatcgcg ccagtcggcc acgagcaacg gcgcgacctg gccggcgcag
      421 gcgtcggcga cgctgtcgac ggcgagaaag cgcacgccca gcggcgcacc ggccagggcg
      481 agcatgcgcg caagctggcc accgccgagg atgccgatcg tgctcatcgt gctgcgctca
      541 cgacgcgcgc gggtcggact tcgccagtac ggcatcggtc tgcgcggcgc ggaattcgtc
      601 cagccgccgc gcgacgcctg cgtcttcgcc ggcgagcagt gccgcggcga acagtgccgc
      661 gttcgtcgcg ccggccttgc cgatcgcgaa cgtggccacc ggaacgccgg ccggcatctg
      721 cacgatcgaa agcagcgaat ccatgccatt cagagcctgc gattgcaccg gtacgccaag
      781 taccggaacg ctggttttcg ccgccagcat tccgggcagg tgcgcggcgc cgccggcgcc
      841 ggcgatgatc gcgcgcaggc cacgcgctcg cgcctggctg gcgtagtcga acagcaggtc
      901 cggcgtgcgg tgcgcggaga cgacgcgcac ttcgtgcgcg atgccgaggc ggtcgagggt
      961 gtccgccgca tgttgcagcg tgtcccagtc cgaacgtgaa cccatcacca cgccgatgcg
     1021 cgggtgcagc gcctttgcgg tgttcatggc ctgtgccctt caaaacggat attctaagga
     1081 ctaactgcaa ctggcgatac ttccgaatgg acaagcgctt gctcgacatc ctgtgctgcc
     1141 cggcgacgaa acagccgttg cgaccgctga cgcgcgccga actcgacgcg ctcaaccgcg
     1201 tcattgccgg cggtggcgtg cggacgacgg cgcaggcgac ggtagatgcg gcgttcgcgg
     1261 cgggtttgat cacggtcgac ggcaagcgcg tctaccgcat cgacgacgac attccggtga
     1321 tgctggcgga tgaagcgatc atggtgtcgc aactggacgg gtttcccgcg cattgatccc
     1381 gtccgtcggc gcgatcctgc gtccggcgga tatatcctcg taacgtgaac tgcttcacaa
     1441 ttccgtacat ggccggactt atgctttacg aagtatggaa caggcagacg ggcacgggtg
     1501 tccggtgtaa tccccgcgat gaacaaacct gacgacacca gcaagatcgt cgatttgcag
     1561 caacgtcaat cgatgcaggc ggccggtggc gaccgcctcg gcgatctgct gcgcgcggtg
     1621 cgcgctaccg cgctgaagcg cctgcacggc ctggtcgcga ccctgttcga aaacatcgac
     1681 gacgccttgt tcgatctggc cgaaaaagcc gagaacaacg cgattcagac ccagtatttc
     1741 gacggcatgc gcgaggtgcg caaaaagcgc cagctcttcg agcgcgtgta ccaggagcag
     1801 ctcggacgcc tcttcaacga tttcgccaac ggcaaattgc aggcgaaccg gccagatccc
     1861 agcaaagccg aaggcgacgg tctttcgctg gtcgaggaca gcgaattgga agaatcgctg
     1921 gccatcgcca gcgcggtagc gaagaacgaa ggccgcctgg cacggcctct
//
LOCUS       DASRVO010000570         5285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_224846, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000570 DASRVO010000000
VERSION     DASRVO010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224846"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFF05_07035"
     CDS             complement(<1..567)
                     /locus_tag="VFF05_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51595.1"
                     /translation="MSVAPKLCLLVIGVLPLGWTTASPAATFCVSTPGQLAAALSAAQ
                     ANGEDDIVYVVAGTYTLTGALEFNSSEAHALTVLGGLDATCSTVTNSFSTLSGQHLYR
                     ALYISSGNGPVAISRMGFQSGSASDLDGGAGLYVFANGDVYIGSSRFLSNNGSLGGGV
                     NAGSNNGQLAFVGNLVANNHAMDFGAGSL"
     gene            complement(564..1919)
                     /locus_tag="VFF05_07040"
     CDS             complement(564..1919)
                     /locus_tag="VFF05_07040"
                     /inference="COORDINATES: protein motif:HMM:NF025670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HZW51596.1"
                     /translation="MRLLTDRKHRLGLAVLPLLLASIAHADTWFPLSQQWLGQNGYSL
                     GSSIAVGVGVDAQGRTVANRVYVGQPYASDGTLMECGSVQVYVPGAGGWVPAATLYAS
                     LRQAGAHFGASLAYGSGHLLVGAPDYNSVGGVGAGAGYVEFFFDNGAATPAIVSSDSR
                     IGNGGNFGSAVAVNIDMAAVGIPNAGGGDGCVAGFHYNLATSNWNNLPALNDIVCGSG
                     GEELGASVAIQRTGDSSYLLVAGAPGQTQNGNLLAGGAHVFFPNPDTGTGGLLEVGTL
                     AADSPAAFDFFGTSVGIDNQYIYVGATGRDNGAGRVGSVTIFKPASIIGYDYLSEYFP
                     GPPATVGGHCGASLSVDAANSQFILGCPDSDDIYYTKAGTARVFRQISFLGQPVWLES
                     VLSYGTQPHGTNFSLGRSVAIIGNQAFAGAPNAHVTGQTGNGAWKAFQPDQLFKDGFG
                     P"
     gene            complement(1971..2540)
                     /locus_tag="VFF05_07045"
     CDS             complement(1971..2540)
                     /locus_tag="VFF05_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZW51597.1"
                     /translation="MRSVKPEVAAKTNFRTERVWQQDRAARERLYANCLPVLRRWARQ
                     RFASCGIHDADDLVQIALLRTLQRLDEIDVRGSGGLLAYLRQIVINEVRSEWRRQVRL
                     GEQSVANESWAADDDPVFNAALSHERDRTWVNALSRLSGYQRAHLSLRVEHGMSFAEI
                     AREIGSSTDAARMVVARATRVLNAQIAAA"
     gene            complement(2674..3630)
                     /locus_tag="VFF05_07050"
     CDS             complement(2674..3630)
                     /locus_tag="VFF05_07050"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZW51598.1"
                     /translation="MGTRLSSIQIESFMMLARELNFSRASESLHITQPALTKRIQGLE
                     EALGQALFVRLHGGLELTESGRVLQRYGATAEHHEAELLASLVGGGADQTLGGFFRIA
                     SFSSALRSVVVPALGTLLRANPRLSCQTFKTEVNELHDWLTDGKVDFVVSLSECERMG
                     VENVQVGIERNVLIESAQYSGREDCYIDHEPRDNFTEKFFLSQTGATVPKMLRAYFDD
                     IYGLIDAVSEGIGRAVVPAHLVKPEHNVRAVAGYRPFEVPVILQFHKQPYYTRLQRAV
                     IDTLTRECPALLARNRMQGTGDGAIADDALVAANRRRVRAQP"
     gene            complement(3751..>5285)
                     /locus_tag="VFF05_07055"
     CDS             complement(3751..>5285)
                     /locus_tag="VFF05_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51599.1"
                     /translation="QRYGTVAQLRADLLRWLEHRPLTIARPGLRYVAARFVRRNRVAV
                     AIAAIALLALIGTTAVALWQAQQAMRMAVRAEHSKDFLAGLLTDANPFQSTHGGKNSI
                     ALLDSAAQRIDKEFSDAPDQQVELRQIIADALSRLGEPKLANDLQRRSVEQIRQLRGE
                     RSPQFGAALGVLAQTAEDSGDIETARAQFEAAYTILQSAGNAYRKQRMTAMTGLAKMA
                     NRRSDFAEGQRWYERVLQERLAQEGPASQDIAMDLFNLGACALYQERYAQATDLMRRA
                     HAMLERLLGAAHPRFIYVDLGLGVAQMKSGHIDDAIKTLTGTLNLARANLKPGAEMVG
                     TIQSALANAQWCAGDDAAAIASAQAARGILAAAHAPSLGVAELTLGRAQLRAHKPQAL
                     RTLADSRQHLRADSARAAGNAKFLALAQAAQGAAFAQGGNAAQGEQEARAARTNLLAV
                     YPAGGEKLGEIDAYLADILAAQGKAAAAHDLRVEALATFQRVYGDAHPVTRALALQIA
                     SR"
BASE COUNT          864 a         1666 c         1817 g          938 t
ORIGIN      
        1 gagcgagccg gcgccgaagt ccatcgcgtg gttgttcgcc accagattgc caacgaatgc
       61 gagctggccg ttgttgctgc ccgcgttcac gccgccgcca aggctgccgt tgttgctcag
      121 gaagcgactg gagccaatgt acacatcgcc gttggcaaac acgtacaagc ccgcgccgcc
      181 gtcgaggtcg ctggcgcttc cggattggaa gcccatgcgc gaaattgcca cagggccatt
      241 gccgctggag atgtacagcg cgcggtacag atgctgtccg ctcagcgtcg agaacgaatt
      301 ggtgacggtg gaacaggtcg cgtccagtcc gccgagcacg gtcagtgcat gcgcttcgct
      361 ggagttgaat tccagtgcgc cggtcagcgt gtaggtgccg gcgacgacat agacgatgtc
      421 gtcctcgccg ttggcttgcg ccgccgacag cgcggcggcg agctgcccgg gagtggaaac
      481 gcagaacgtc gccgccgggc tggcggttgt ccacccgagc ggcaggacgc cgatgacgag
      541 caggcacagc ttcggcgcaa cgctcatggt ccgaaaccgt ccttgaagag ttggtccggc
      601 tggaatgctt tccacgcgcc attgccggtt tggcctgtga catgggcgtt cggcgcgccg
      661 gcgaaggcct gattgccgat gatcgccacg ctgcggccga gcgaaaaatt ggtgccgtgc
      721 ggctgcgtgc cgtagctcag cacgctttcc agccacaccg gttggccgag gaaactgatc
      781 tggcgaaaca cccgcgcggt gccggccttg gtgtagtaga tgtcgtcgct gtccgggcag
      841 ccgaggatga actgtgagtt ggcggcgtcc acgctcaagg acgctccaca atgcccaccg
      901 actgtggctg gaggtccggg gaaatattcg gacaagtagt cgtaaccgat gatcgaggcg
      961 ggtttgaaga tggtcaccga accgacgcgc ccggcgccgt tgtcgcggcc ggtggcgcca
     1021 acgtagatgt actggttgtc gatgccgacg ctggtaccga agaagtcgaa tgcggccgga
     1081 ctgtccgcgg ccaatgtccc cacttccaac aagccgccgg tgccggtgtc tggattcggg
     1141 aagaatacgt gtgcgccgcc agccagaaga ttgccattct gggtttgccc cggcgcaccg
     1201 gcgacgagca gataactgct gtcgccggta cgctggatcg ctaccgatgc gccgagttct
     1261 tcgccgccgc tgccacagac gatgtcgttc agcgccggca ggttgttcca attcgacgtg
     1321 gccaggttgt aatggaaccc cgcgacacag ccgtcgccgc cgccggcatt gggaatgccg
     1381 accgcggcca tgtcgatgtt taccgcgacc gcgctgccga aattgccgcc gttgccgatg
     1441 cgcgagtcgg aggacacgat ggccggtgtg gcggcgccgt tgtcgaagaa gaattcgaca
     1501 tagccggcgc cggcaccgac gcctccgacg gagttgtaat cgggggcgcc gacaagcaga
     1561 tgccccgatc cgtaggccag gctcgcgccg aaatgcgcac cggcctggcg caagctggcg
     1621 tacagcgtcg ccgccggaac ccagccgccc gcgccgggca catacacctg cacgctgccg
     1681 cattccatca gtgtgccatc gctcgcgtac ggctggccga cgtagacccg gttggcgacg
     1741 gtgcggccct gcgcatccac gccgacgccg acggcgatgc tggaccccag gctgtagccg
     1801 ttctgcccca accattgctg gctcagcggg aaccaggtat cggcgtgcgc gatgctagcg
     1861 agcagcaatg gcaacacggc gagtcctagg cgatgtttac ggtcggtgag aaggcgcatg
     1921 gcggtttcct cggcagtggt ttttacttgg ctccgggagc aatgtcggaa tcaggcggcg
     1981 gcaatctgtg cattcagcac gcgcgtggcg cgcgccacga ccatgcgggc ggcatccgtg
     2041 ctgctgccga tttcgcgtgc gatctcggcg aaactcatgc catgttcgac gcgcagggat
     2101 agatgcgcgc gctggtaacc gctgagcctg gacaacgcat tgacccatgt gcgatcgcgt
     2161 tcgtgggaca gtgcggcgtt gaacacgggg tcgtcgtcgg ctgcccaaga ttcattggcg
     2221 acagattgtt cgccaagacg aacctgacgc cgccattcgc tgcgcacctc gttgatcacg
     2281 atctggcgca ggtaggccag cagaccgccg ctgccgcgca cgtcgatctc atccaggcgc
     2341 tgcagggtgc gcaacagggc gatctgcacc aggtcgtcgg cgtcgtggat gccgcaactg
     2401 gcaaaacgtt ggcgcgccca gcgacgcaat accggcaaac agtttgcgta caggcgctcg
     2461 cgtgcggcgc gatcctgctg ccatacgcgc tctgtgcgga aattggtttt tgccgcgact
     2521 tccggtttta cgcttcgcat gtgccgagcc cgttcgatgt caacgggggc gcagtgtgga
     2581 aacgcggcga gcgcgcgtcc aatgaaacat cgctatgcgg cgatgcggag gacttatgcc
     2641 gataacccgg tggaatcggc ttatcgccgc cggttatggc tgggcgcgca cgcgccgccg
     2701 gtttgccgca accagtgcat catcggcgat ggcgccatct ccggtgccct gcatgcggtt
     2761 gcgcgcgagc agggcggggc attcgcgcgt gagcgtgtcg atcaccgccc gctgcagccg
     2821 tgtgtagtaa ggctgcttgt ggaattgcag gatcaccggt acttcgaacg gccggtagcc
     2881 ggcaacggcg cgcacgttgt gctccggctt gaccaggtgc gccggcacca cggcgcggcc
     2941 gatgccttcg gacaccgcgt cgatcaggcc gtagatgtcg tcgaaatagg cgcgcaacat
     3001 cttcggcacg gtggcgccgg tctggctcag gaagaacttc tcggtgaagt tgtcgcgcgg
     3061 ctcgtggtcg atatagcaat cctcgcgccc ggaatattgc gccgactcga tcagtacgtt
     3121 gcgttcgatg ccgacttgca cgttttccac gcccatgcgt tcgcactcgg acagcgagac
     3181 gacgaaatcg accttaccgt cggtgagcca gtcgtgcaac tcgttgactt cggtcttgaa
     3241 cgtctggcag gacaggcgcg ggtttgcgcg cagcagggtg ccgagcgcgg gcacgacgac
     3301 agaacgcagc gcggacgaaa acgaggcgat gcggaagaat ccgcccaggg tctggtcggc
     3361 gccgccgccg acgaggctgg ccagcagttc ggcttcatgg tgctcggcgg tcgcgccgta
     3421 gcgctgcagc acgcggcccg attcggtgag ttcaaggccg ccgtgcaggc gcacgaacaa
     3481 tgcctggccg agggcttcct ccaacccctg gatgcgcttg gtcagcgccg gctgggtgat
     3541 gtgcagggat tcggacgcgc gcgaaaaatt caactcgcgc gcgagcatca tgaacgattc
     3601 gatttgtatc gacgacaacc gcgttcccat ggcgccggtc tccccacatg ctccgtgcaa
     3661 tcataacagc ggggcatcgc gccattcgtg aaagttatcg cgattcccgc ggcgcgactt
     3721 gcggcggaat ttccgcgggc aacgcgcagc ttaccgcgaa gcgatttgca gagccagcgc
     3781 gcgcgtaacc ggatgcgcat cgccgtacac gcgctggaac gtggccagtg cctccacgcg
     3841 caggtcgtgc gcggcggccg ctttgccttg cgcggcgagg atatcggcca ggtaggcgtc
     3901 gatctcgccc agcttctcgc cgccggcggg ataaaccgcc agcagattcg tgcgcgccgc
     3961 gcgtgcctct tgctcgccct gcgcggcatt gccgccctgg gcgaaagcgg cgccctgcgc
     4021 ggcttgcgcg agggcgagga atttggcgtt gccggccgca cgcgcactgt cggcgcgcag
     4081 atgctggcgg ctgtccgcca gcgtacgcag cgcctgcggc ttgtgggcac gtagctgcgc
     4141 acggccaagg gtcagttcgg cgacgcccaa gctcggcgcg tgcgcggcgg ccaggattcc
     4201 gcgcgcggcc tgtgccgacg cgattgcggc cgcatcgtcg ccggcgcacc actgtgcgtt
     4261 cgccagtgcc gactggatcg tgccgaccat ctcggcaccg ggcttcaggt tcgcgcgggc
     4321 gaggttcagg gtgccggtca gggtcttgat cgcgtcgtcg atgtgccccg atttcatttg
     4381 cgctacgccg aggccgagat cgacatagat gaaacgcgga tgggcggcgc cgagcaagcg
     4441 ttcgagcatg gcgtgcgcgc gccgcatcag atcggtggct tgtgcatagc gctcctgata
     4501 cagcgcgcaa gcgccgagat tgaacaggtc catggcgatg tcctggctcg cggggccttc
     4561 ctgcgccagc cgttcctgca atacccgctc gtaccagcgc tggccctcgg caaaatcgct
     4621 gcgccggttc gccatcttgg ccaggccggt cattgcggtc atgcgctgct tgcgataggc
     4681 gttgcccgcg gattgcagga tcgtgtaggc ggcttcgaac tgcgcgcgcg cggtttcgat
     4741 atcgccgctg tcctcggcgg tttgcgccag cacgccgagc gccgcgccga actggggcga
     4801 gcgttcgccg cgcagttggc ggatctgttc gacgctgcgt cgttgcaggt cgttggcaag
     4861 cttgggctcg cccaggcgcg acagtgcgtc ggcgatgatc tggcgcaatt cgacttgctg
     4921 gtccggcgcg tcggagaatt ccttgtcgat gcgctgcgcg gcgctgtcga gcagcgcgat
     4981 gctgttcttg ccgccgtggg tggactggaa cgggttggcg tccgtgagca agcccgcgag
     5041 aaagtccttg ctgtgttcgg cacgcaccgc catgcgcatg gcctgctgcg cctgccacag
     5101 cgcgacggcg gtggtgccga tcaaggcgag cagggcaatg gcggcgatcg ccacggcgac
     5161 gcggttgcgg cgcacgaagc gcgccgcaac atagcgcagg ccggggcgag cgatcgtcag
     5221 tggccggtgt tcgagccagc gcagcaggtc cgcgcgcagt tgtgcgacgg tgccgtagcg
     5281 ctgcg
//
LOCUS       DASRVO010000571         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_224863, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000571 DASRVO010000000
VERSION     DASRVO010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224863"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFF05_07060"
     CDS             <1..630
                     /locus_tag="VFF05_07060"
                     /inference="COORDINATES: protein motif:HMM:NF020467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1839 family protein"
                     /protein_id="HZW51600.1"
                     /translation="DVDGRRLGYFHNGGYFQLAGEDFARLFRLGAAPDPAFMPLFAEL
                     VRVDRRVRRPERELAAMSYALLRRHAGWRPPDNPFVRFASRFERDLSQLRTRGLAHYH
                     AWAFASVRQAGAAFELAASHLEWLAQLNHPGLRAAGESFNAISQANKALILKGARAVH
                     TGKPLDATALVAAMAQAWDEGMARIDDCLETDGPMPSTMDEDEGVRFGQ"
     gene            734..1486
                     /locus_tag="VFF05_07065"
     CDS             734..1486
                     /locus_tag="VFF05_07065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZW51601.1"
                     /translation="MFERLQGWTDPGEQAAVEHVAAQVRSRPILDIGVGAGRTTTLLR
                     AISCDYVGIDYTKEMVDACRAKHPDARIEHMDARDLSAFADNQFALVVFSFNGIDAVE
                     LADRHKILREVHRVLQPGGLFIVSAHNRNGPGFGEQPHLHLRFSWNPLKLGWRTWQLA
                     RSLVPTWRNRHRLRALNEAHDEWAIMNAGAHDFGIVIMYTTLAEQKRQLAAAGFTTEL
                     VLDNVNGQPVSDGADTHAMWWFHFIARKPAVA"
     gene            1662..2132
                     /locus_tag="VFF05_07070"
     CDS             1662..2132
                     /locus_tag="VFF05_07070"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZW51602.1"
                     /translation="MHQTLTQEVAAAPSGTGPVRLGFDLVQISRIEDSVRHFGEAFEQ
                     RLFTPDERAYAHDGTGVAAQRLAARFAAKEAVIKALRLAEAGIDWRDIEVCRLPDGDC
                     SIRLHGRARELAQAQGARAILVSLSHDGDYAGAVANVLGSSPTPTEHSSPPPQS"
     gene            2129..2392
                     /locus_tag="VFF05_07075"
     CDS             2129..2392
                     /locus_tag="VFF05_07075"
                     /inference="COORDINATES: protein motif:HMM:NF005480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZW51603.1"
                     /translation="MTTSTTLQAETQIRDVLRAHGRLNRDVDQVAVGDDLYQSGLTSH
                     ASVNVMLALESAFDVEFPDRMLKRSVFASIESIGRAIDELRSA"
     gene            2417..>3035
                     /locus_tag="VFF05_07080"
     CDS             2417..>3035
                     /locus_tag="VFF05_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51604.1"
                     /translation="MRQRFLLDAGWSICSAPAASSEAPSGQARWLPIREAAPVAAALR
                     ERGEWSLDAPPVDFDASDWWYRLRFDRPPLARDTDVVLGFDGLATLAQVQCNGTPLPA
                     SDNMFVRSEHDITRLLRDRGNELLIRCCALGAELARRRPRPRWRTPMVAHQQLRWFRT
                     TLLGRTPGWSPPAAVVGPWRPVWLAQGGVIDPADLQLDISVEGGAG"
BASE COUNT          480 a         1016 c         1043 g          496 t
ORIGIN      
        1 gatgtcgatg gccggcggct gggatatttc cacaatggcg gctatttcca actggcgggc
       61 gaagactttg cccggttgtt tcggctgggc gccgcgcccg atccggcttt catgccgctg
      121 ttcgccgagc tggttcgcgt cgacaggcgc gtgcggcggc ccgagcgcga gctcgcggcg
      181 atgtcgtacg ccctgctgcg gcgccatgcc ggttggcgcc cgcccgacaa cccgttcgtg
      241 cgcttcgcct cgcgcttcga gcgggacttg agccaactgc gcacgcgcgg cctggcccac
      301 taccatgcct gggccttcgc ttcggtgcgg caggcggggg ccgcgttcga attggccgcg
      361 tcgcacctcg aatggctggc gcagctgaac caccccggcc tgcgcgcggc gggcgaatcc
      421 ttcaatgcga tctcgcaggc caacaaggcg ctgatcctca agggtgcgcg ggccgtgcac
      481 accggcaagc ccctggacgc gacggcgctg gtcgccgcga tggcgcaggc atgggacgag
      541 ggcatggcgc ggatcgacga ttgcctggaa accgatggcc cgatgccgtc gacgatggat
      601 gaagacgaag gagtccgatt tggccaatag tctgtttacc gcagaaagca acccggcacc
      661 gcgagcgccg ctgccgcatc gcgatctgga cgtggtcaat cgcggcgcat ggaaggcgcc
      721 cgggacggtt cacatgttcg agcgcctgca aggatggacc gatccgggcg aacaggcggc
      781 ggtggagcat gtcgccgcgc aagtgcgatc gcgcccgatc ctggacatcg gcgtgggtgc
      841 cgggcgcacg accacgctgc tgcgggcgat cagctgcgac tacgtcggga tcgactatac
      901 gaaggagatg gtcgacgcat gccgcgccaa gcaccccgat gcgcgaatcg agcacatgga
      961 cgcgcgcgac ttgagcgcct tcgcggacaa tcagtttgcg ctcgtcgtgt tctcgttcaa
     1021 cggcatcgat gccgtcgaac tggccgaccg ccacaagatc ctgcgcgaag tgcaccgggt
     1081 gctgcaaccg ggcggcctgt tcatcgtctc cgcgcacaac cgcaacggcc cggggttcgg
     1141 cgaacagccg cacctgcatc ttcgtttcag ctggaatcct ctcaagcttg gctggcgtac
     1201 gtggcagttg gcccgctcgc ttgttccgac gtggcgcaac cgccatcgct tgcgcgcgct
     1261 caacgaggct cacgatgagt gggcgatcat gaatgccggc gcgcatgact tcggcatcgt
     1321 gatcatgtac acgacgctcg ccgagcagaa gcggcagttg gcggcggcgg gattcaccac
     1381 ggagctggta ctggacaacg tcaacggcca gccggtgagc gacggcgccg atacccatgc
     1441 gatgtggtgg tttcacttca ttgcccgcaa gccggcggtc gcgtgaaacc ccgtcgagcc
     1501 cgcaagtgac cggcacggcc gcgcactgct cgctgcgaag gcgcggatgc tgtgctacac
     1561 ggtcatgagg cgctgtccga catgctgcgg aaatcgcctc agctattctg gcggcaatcg
     1621 cctcgacgct gttggaactg caaggaattc ggaacccggg tatgcaccag acgctcacgc
     1681 aggaagttgc tgccgcgcca tccggcacgg ggcccgtgcg cctcggattc gatctcgtgc
     1741 agatcagccg tatcgaggac tcggtgcggc atttcggcga ggcgttcgag caacgcctgt
     1801 tcacgccgga cgagcgcgcg tatgcccatg acggcaccgg ggtcgccgcc cagcggctgg
     1861 cggcgcgctt cgcggccaag gaagcggtca tcaaggcgct gcggctggcc gaagccggca
     1921 tcgactggcg cgacatcgag gtctgccgct tgcccgatgg cgattgctcg atccggctgc
     1981 acggccgtgc gcgcgagctg gcgcaggcgc agggcgcacg cgccattctg gtcagcctga
     2041 gccacgacgg cgactatgcc ggcgcggtcg ccaatgtcct gggctcgtcg cccactccaa
     2101 cggagcactc ttcaccaccg ccgcagtcat gaccacttcg accacgctcc aagccgaaac
     2161 acagatccgt gacgtgctgc gcgcgcacgg gcgtctcaat cgcgacgtcg atcaggttgc
     2221 ggtcggcgac gatctgtatc agtcaggcct cacgtcgcat gcgagcgtga acgtgatgct
     2281 cgcgctcgaa tccgccttcg acgtcgagtt tccggaccgc atgctcaagc gcagtgtgtt
     2341 cgccagcatc gagtcgatcg gccgcgcgat cgacgagttg cgatcggcct gaaggccgct
     2401 gccgatggac gtgccggtgc gtcagcgctt cctgctcgac gcaggctggt cgatctgcag
     2461 cgcaccggcc gcaagcagcg aagcgccgtc cggccaggcg cgctggttgc cgattcgcga
     2521 ggcggcgccg gtggccgccg cgctgcgcga gcgcggcgaa tggtcgctcg atgcgccgcc
     2581 agtggacttc gatgccagcg attggtggta tcgcttgcgc ttcgatcgtc cgccgctcgc
     2641 ccgcgacacg gatgtggtgc tcggcttcga cgggctggcc acgctggcgc aggtgcaatg
     2701 caatggaacg cccctgccgg ccagcgacaa catgttcgtg cgcagcgagc acgacatcac
     2761 gcggctgttg cgcgaccggg gcaatgaact gctgatccgc tgctgcgcgc tcggcgcgga
     2821 actcgcacgc cgccgcccgc ggccgcgctg gcgcacgccg atggtcgcgc accagcaact
     2881 gcggtggttc cgcacgacac tgctcggacg cacgccggga tggtccccgc cggccgcggt
     2941 ggtcgggccg tggcgcccgg tgtggctggc gcaaggtggc gtcatcgacc cggcggatct
     3001 ccaactcgac atctcggtcg aaggcggcgc aggcc
//
LOCUS       DASRVO010000572         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_303266, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000572 DASRVO010000000
VERSION     DASRVO010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303266"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFF05_07085"
     CDS             <1..489
                     /locus_tag="VFF05_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZW51605.1"
                     /translation="IKHGLESAPAVAGRLIRHAMPGGWTLIDDSYNANPGSTAAAIAT
                     LAAEASSTKSGESWLVLGDMRELGADASKLHADIGDLAREQGIARLYAVGDLSKHAAR
                     AFGANARHFADQSALIAALAADAHAGVTVLVKGSRGSAMDRVVRALLGEANGNGGARH
                     AA"
     gene            479..>1233
                     /gene="mraY"
                     /locus_tag="VFF05_07090"
     CDS             479..>1233
                     /gene="mraY"
                     /locus_tag="VFF05_07090"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZW51606.1"
                     /translation="MLLELADLLRGHLSAFHLFQYVSFRTIMSALTALCVALLLGPNL
                     IRKLAAVKAGQVIRSDGPQSHLSKAGTPTMGGALILLAVAVATLLWGDLRNRYLWIVL
                     GVTLAFGLIGFYDDYRKLVFRDSRGLPARWKYFWQSVCGLGAALGLYFVLRQDSPAAL
                     ALYVPLFKQVAIPLGIGFVAIGYFIIVGFSNAVNLTDGLDGLAIMPTVLVASALGVFA
                     YVAGHSVFSQYLQIPAVPGAGELCIFCGALAGAG"
BASE COUNT          169 a          400 c          418 g          246 t
ORIGIN      
        1 atcaaacacg gactggaatc cgcacccgcc gtggctgggc gcctgatccg ccacgccatg
       61 cccggtggtt ggaccctgat cgacgacagc tacaacgcca atcccggttc gaccgcggcg
      121 gcgatcgcca cgcttgccgc cgaagcatct tcgacaaaat cgggcgaatc ctggctcgtg
      181 ctcggcgaca tgcgcgagct gggggccgat gcgtcgaagc tgcacgcgga catcggcgac
      241 ctcgcgcgcg aacagggcat cgcgcgtctg tatgcggtcg gcgatctgtc caaacacgcg
      301 gcgcgggcgt ttggcgcgaa cgcgcgccat ttcgccgatc agtccgcgtt gatcgcggcg
      361 ctggccgccg atgcgcatgc cggcgtgacc gtgttggtca aaggttcgcg tggctcggcg
      421 atggatcggg tcgtgcgcgc cttgctcggc gaagccaacg ggaatggagg cgcgcgccat
      481 gctgcttgaa ctcgccgact tgctgcgcgg ccacctgagc gccttccatc tgttccagta
      541 cgtgtcgttc cgcacgatca tgagcgcgct gaccgcgctc tgcgtcgcgc tgctgctcgg
      601 gccgaacctg atccgcaagc tggcggcggt gaaagcgggg caggtgatcc gcagcgatgg
      661 tccgcagtcg cacctgtcca aggccggcac gccgacgatg ggcggcgcgc tgatcctgct
      721 tgccgtcgcg gtggcgacat tgttgtgggg cgatctgcgc aaccgctatc tgtggatcgt
      781 gcttggcgtc acgctggcgt ttggcctgat cggcttctac gacgattacc gcaagctcgt
      841 gttccgcgac agccgcggcc tgccggcacg ctggaaatat ttctggcagt cggtgtgcgg
      901 actcggcgcg gcgctgggtc tttatttcgt gttgcgccag gattcgcccg ccgcgctcgc
      961 gttgtacgtg ccgctgttca agcaggtcgc gattccgctc ggtatcggtt tcgtcgcgat
     1021 cggctatttc atcatcgtcg ggttttccaa cgcggtgaac ctgaccgatg gattggacgg
     1081 cctcgccatc atgcccaccg tgctcgtcgc ctccgcgctc ggcgtgttcg cctacgtcgc
     1141 gggccacagc gtgttctcgc agtatctgca gattccggca gtgcccggcg ccggggagtt
     1201 gtgcatcttc tgtggcgcgc tggccggagc ggg
//
LOCUS       DASRVO010000573         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_303311, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000573 DASRVO010000000
VERSION     DASRVO010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303311"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFF05_07095"
     CDS             <1..536
                     /locus_tag="VFF05_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008213000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZW51607.1"
                     /translation="RYSPWLRRLHWLIALLIVAAWALSEARGYTQRGSDLRAAVTWTH
                     IQFGLAVILLMLPRLLARLRSATPPITPPIAIWQAWLAKLMHLALYALAFFVPVLGMA
                     MQYTKGASWDLFGLPLPVSASPDRDLAHQLEELHETCGNVLFWLAMVHAAVALFHHWV
                     TRDNTLRRMLPVLRSED"
     gene            607..1476
                     /locus_tag="VFF05_07100"
     CDS             607..1476
                     /locus_tag="VFF05_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZW51608.1"
                     /translation="MNERRYSAPSGIPALIGIIVLLIADGAWFVYSIQNQSVWAIIVA
                     ILAAIVLLICLGGFFTVAPNEGKVLQFFGNYVGTVRDAGPRWTNPFYSKRGISLRVRN
                     FESSKLKVNDSDGNPIEIAAVVVWQVVDTAEALFQVNDYEDFVHIQSESALRQMAQTY
                     PYDSHDTGGVSLRSHGKEIVEHLQNEIQERLASAGVKVIEARISHLAFAPEIAQAMLQ
                     RQQASAIIAARTRIVEGAVTMVEMALDQLKTRGVVQLDEERKAAMVSNLLVVLCGERG
                     TQPIVNTGTLYSG"
BASE COUNT          289 a          533 c          490 g          329 t
ORIGIN      
        1 atcgctattc gccgtggctg cgccgcctgc actggctgat cgccctgctg atcgtggccg
       61 cctgggcact ctccgaagca cgcggctata cgcaacgcgg ttccgatttg cgcgccgccg
      121 tcacctggac gcacatccag ttcgggcttg cggtgatcct gctgatgctg ccgcgcctgc
      181 tcgcgcgcct gcgctcggcg acgccgccga tcacgccgcc tatcgccatc tggcaggcgt
      241 ggctggccaa actgatgcat ctggcgctgt acgcgctggc cttcttcgtg cccgtcctcg
      301 gcatggccat gcaatacacc aagggcgcga gttgggatct gttcggcttg cccctgccgg
      361 tcagcgccag tccggatcgc gacctcgccc accagctcga agagttgcac gaaacctgcg
      421 gcaacgtatt gttttggctg gccatggtgc acgccgccgt ggcgcttttc caccattggg
      481 tgacacgcga caatacactc aggcgcatgc tgccggtatt gcgttcggaa gactgaacgg
      541 gtcggtcggt ttgcaaatat gatatcttta tgatatcttt atttcgcaaa ccaaccggag
      601 cccgccatga acgaacgccg ttattccgcc ccttcgggca ttcccgccct gatcggcatt
      661 atcgtgttgt tgatcgccga cggcgcgtgg ttcgtctact cgatccagaa tcaatccgtc
      721 tgggcgatca tcgtcgccat cctcgccgcc atcgtgttgc tgatctgtct cggcggcttc
      781 ttcaccgtcg cgccgaacga gggcaaggtg ctgcaattct tcggcaacta cgtcggcacg
      841 gtgcgcgacg ccggcccgcg atggaccaat ccgttctact ccaagcgcgg catttcgctg
      901 cgcgtgcgca acttcgagtc gagcaagctc aaggtcaacg attcggacgg caatcccatc
      961 gagatcgccg ccgtggtcgt ctggcaagtc gtcgataccg ccgaagcgct gttccaggtc
     1021 aacgactacg aggacttcgt gcatatccag agcgaatccg cgctgcggca gatggcacaa
     1081 acctaccctt acgattcgca cgataccgga ggcgtctcgc tgcgcagcca cggcaaggaa
     1141 atcgtcgagc acctgcagaa cgaaatccag gaacgcctcg ccagcgccgg cgtcaaggtg
     1201 atcgaggcgc gtatttcgca tctcgcgttt gcgccggaaa tcgcgcaggc gatgctgcag
     1261 cgccagcagg ccagcgcaat cattgctgca cgcacgcgca tcgtcgaagg tgctgtgacc
     1321 atggtcgaga tggcactgga tcagctcaag acgcgcggcg tcgtgcagct cgacgaggaa
     1381 cgcaaggcgg cgatggtgtc gaacctgctc gttgtgttgt gcggcgaacg cggcacccag
     1441 ccgatcgtca ataccggcac gctgtattcg ggctgatccg gcatggaact cgaatccatg
     1501 acaccgttcc tgctgctggc ggcgagtttc gccgccggcc tcagcggact gctggtgctc
     1561 gcacacaacc acgacgacat cgggcggcac ggcgatttgt cgacgcgaaa tcgacgcatc
     1621 gcgcgtgcgc tgttcgtatt g
//
LOCUS       DASRVO010000574         1183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_120844, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000574 DASRVO010000000
VERSION     DASRVO010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120844"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFF05_07105"
     CDS             <1..852
                     /locus_tag="VFF05_07105"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HZW51609.1"
                     /translation="CDAYSFGGGKYPIAAGGGIFAGTVTLTNGSQVSRNTATGVDFTV
                     HTFGAGVFANSQLTCTDSTLSGNITLLRGGGAASAGSTSITRCTIDSNMAGYGAGLYQ
                     FGTSGTVAIHESTISGNQGGKGGGIYSKAPLTIDNSTVAFNSGIASNGGGIHSTANII
                     ATSSIFARNTNTNGVNPDVVLVSGKTLTGSGDLVMSINNLPLPGVIATSLDPRLAPLG
                     NHGGPTPTHALLAGSPAIDAGGNPLADTSDQRGAGFAREVPTGAPDIGAYERQPGEDE
                     IFGNGFD"
     gene            979..>1183
                     /locus_tag="VFF05_07110"
     CDS             979..>1183
                     /locus_tag="VFF05_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZW51610.1"
                     /translation="MIEIPGYKILRQLGRGGMATVYLAIQESVQREVALKVMSPSLLA
                     DPDFGERFLREARIAAKLHHRNVV"
BASE COUNT          218 a          388 c          378 g          199 t
ORIGIN      
        1 tgtgacgctt acagctttgg cggcggcaaa tatcccattg cggcgggtgg cggaatcttt
       61 gctggaacgg tgacgctcac gaacggcagc caggtgtcgc gcaacaccgc gacgggcgtc
      121 gatttcacgg tgcacacgtt cggcgccggc gtgtttgcga actcccagct cacctgcacc
      181 gacagcacgc tcagcggcaa catcaccctg ctgcgcggcg gcggtgcggc gtcggccgga
      241 tcgacgtcaa tcacccgctg cacgatcgat tccaacatgg caggctatgg cgccggcttg
      301 taccaattcg gtacgtcggg cacggtcgcc atccacgaga gcacgatctc cggcaaccag
      361 ggtggcaagg gcggcggcat ctacagcaag gcgccgctga cgatcgacaa cagcacggtc
      421 gcattcaatt cgggcattgc gagtaacggc ggcggcattc attccaccgc aaacattatt
      481 gcgaccagtt cgattttcgc gcgcaacacc aacacgaacg gcgtgaatcc ggatgtcgtg
      541 ctcgtcagcg gcaagacgct caccggttcc ggcgacctgg tgatgtcgat caacaacctg
      601 ccgcttcccg gcgtgattgc gacttcactc gatccgcggc tggcgccgct cggcaatcac
      661 ggcggaccga cgccaacgca cgcgctactg gcgggaagtc cggcgatcga cgccggcggc
      721 aacccgctgg ccgacacttc ggaccagcgc ggcgcgggtt tcgcgcgcga agtgcccacg
      781 ggcgcacccg acatcggcgc ctacgaacgt cagcccggcg aggacgagat tttcggcaac
      841 gggttcgatt gagcggacgg ccctgcagga tgcgtgatcc gtgaccgacc ggagccacgg
      901 tttgccttga attccgcggg cttgcggccc acggcatgct atgcttcgcg ccggattccg
      961 gcgagagcgg atcgccacgt gattgaaatt cccggctata aaatcctgcg tcagctcggt
     1021 cgcggcggca tggctaccgt gtatctggcg atacaggaat cggtgcagcg cgaagtcgcg
     1081 ctaaaagtga tgtcgccgag cctgctcgcc gatccggact tcggcgagcg cttcctgcgc
     1141 gaggcgcgca tcgccgccaa gctgcaccac cgcaacgtgg tcg
//
LOCUS       DASRVO010000575         1830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_145930, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000575 DASRVO010000000
VERSION     DASRVO010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145930"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..741)
                     /gene="eda"
                     /locus_tag="VFF05_07115"
     CDS             complement(91..741)
                     /gene="eda"
                     /locus_tag="VFF05_07115"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZW51611.1"
                     /translation="MIASMPQKRERLESILRLAPVIAVVTIDDAKAAVPLARALREGG
                     IHAIEITLRTPAALDAIRAIADAIPQAAVGAGTVLSADDLAAATAAGAKFAVSPGASP
                     ALFDAAANSSLPWLPGAASASEAMALAERGYDLQKFFPAQAAGGVEFLRSLAGPLPHI
                     RFCPTGGIGVHNAPDYLLLPNVVCVGGSWLTPATLVRSGDWSAIAALARAAAALRD"
     gene            complement(738..>1830)
                     /locus_tag="VFF05_07120"
     CDS             complement(738..>1830)
                     /locus_tag="VFF05_07120"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZW51612.1"
                     /translation="SFVHPNTPLREALTVEAVRCAARMTALGEHYLPVGHVVDERAIV
                     NGVVGLHATGGSTNHLLHLVAIAHAAGIELRWDDFDALSHAVPLLARVYPNGSADVNA
                     FHAAGGMGFLIAQLLDAGLLHADIRTVNGTGLDAYRIEPRLGANGAVERVPVLRESRD
                     TSVLRSVREPFGADGGLRVLDGNLGRAVIKVSAVPEDRWIVEAPARVFDDQDAVRAAF
                     ERGELDRDFVAVVRFQGPHANGMPELHKLTPLLSVLQNRGFRVALVTDGRMSGASGRV
                     PAAIHVTPEASAGGAIVKIRDGDIVRIDAKYGTLDLKIDAAALAAREPAAYANRDAHG
                     SGLGRELFARFRAAASSADAGASFFGDGA"
BASE COUNT          325 a          631 c          595 g          279 t
ORIGIN      
        1 cgggttgatc cacgtcaaag caggatcgtg atgctggatt ctcggttcgg ctgaggcagt
       61 aatgcgcgcg tacgccccgc aatctcgaaa ctaatcacgc aacgcggcag ccgcgcgcgc
      121 cagcgcggca atcgcgctcc aatcgccgct gcgcacgagc gtcgccggcg tcaaccacga
      181 gccgcccacg cacacgacat tcggcagaag caggtaatcg ggcgcattgt gcacgccgat
      241 gccgccggtc ggacaaaaac gaatgtgcgg caagggtccg gcgagcgaac gcagaaactc
      301 gacgccgccc gcggcctgcg ccggaaagaa tttctgcagg tcgtacccgc gctccgcgag
      361 tgccatcgcc tcgcttgcgc tggcggcgcc cggtagccag ggcaatgagg aatttgcggc
      421 cgcgtcgaac aaggcgggcg atgcgcccgg cgacaccgcg aacttcgcac ctgcggcggt
      481 ggcggcggca agatcgtccg cgctcaacac cgtgccggcg ccgactgcgg cctgcggaat
      541 cgcatcggcg atcgctcgaa tcgcgtccaa tgcggccggc gtgcgcaggg tgatttcgat
      601 ggcgtggatg ccgccttcgc gcagtgcgcg cgccagcggc acggcggcct tcgcatcgtc
      661 gatcgtcacc acggcgatga cgggtgcgag gcgcaggatg gattcgagtc gttcgcgctt
      721 ttgcggcatc gacgcaatca tgcgccatct ccgaagaagc tcgcgccggc gtcggcagag
      781 ctcgcggcgg cgcggaagcg cgcgaacaat tctcgcccca acccacttcc gtgcgcatcg
      841 cgattcgcgt acgccgccgg ttcgcgcgcg gcgagtgccg cggcgtcgat tttcagatcg
      901 agcgtgccgt acttcgcgtc gatgcgcaca atgtcgccgt cgcgaatctt gacgatcgcc
      961 ccacccgcag acgcttccgg cgtgacatgg attgctgccg gcacacgtcc cgaagcaccg
     1021 gacatgcgcc cgtcggtgac cagcgcgaca cggaatccgc ggttctgcaa cacgctgagc
     1081 agcggcgtga gcttgtgcag ttcgggcatg ccgttcgcgt gcgggccctg aaagcgcacc
     1141 accgcgacga aatcgcgatc gagttcgccg cgttcgaacg cggcacgcac cgcatcctga
     1201 tcgtcgaaca cccgcgccgg ggcttcgaca atccagcgat cttccggcac cgccgagacc
     1261 ttgataacgg cacggcccag attgccgtcg agtacgcgca atccaccatc ggcgccgaac
     1321 ggttcgcgca cgctacgcag caccgacgtg tcgcggcttt cgcgcagcac cgggacgcgt
     1381 tcgactgcgc catttgcgcc gaggcgcggt tcgatgcggt aggcgtcaag acccgtgccg
     1441 ttgacggtgc ggatgtcggc gtgcagcaat cccgcatcga gcagttgcgc aatcaggaaa
     1501 cccataccgc ccgcggcatg aaatgcattc acgtccgcgc tgccgttcgg gtacacgcgc
     1561 gccagcaacg gcaccgcgtg cgagagcgca tcgaaatcgt cccagcgcag ttcgatgccc
     1621 gccgcatggg cgatggcgac cagatgcagc agatgattcg tcgatccgcc ggtcgcgtgc
     1681 aggccgacca caccgttgac gatggcgcgc tcgtcgacga catgaccgac cggcagatag
     1741 tgctcgccga gcgctgtcat tcgcgctgcg caacgcaccg cctcgaccgt aagcgcctcg
     1801 cgcagcggcg tattcggatg cacgaagctc
//
LOCUS       DASRVO010000576         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_250664, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000576 DASRVO010000000
VERSION     DASRVO010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250664"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VFF05_07125"
     CDS             <1..1459
                     /locus_tag="VFF05_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZW51613.1"
                     /translation="LTNAWAQEKVAHLDFNGGLFDLPAGTAQLAIGGSYRNEYTNSVD
                     DATLLINPDTGNCTLGSQCSSNLNGGYNVKEAYGELFLPLFKDLPFVNSLNVTLSDRY
                     SKYNTFGSTNNAAIGVEWKPIDDLLLRANYNKVFRAPTVGDIFGNPISDAPKLSSDPC
                     TGITVANPACVGVPLDGSFQNTDVQLGQQIKGIASGSAYAGFPLGPENGATFNWGFVY
                     SPHWVEGLSLSADLWRVYLNNTITSVGAQAVLNNCFAGQLQYCPLITRFGPDSTQPGQ
                     VKQILEPTANLGRLDTKGADFSVKYRLPQFSFGQFAVALDATYLAQYNQESGGVLYHD
                     AGHLLNFGSGAQAACPDLGGGVCLFPRWRALASVNWNLGSWDASWRMRYTGKFRMGSK
                     SPSQDTFPVGTCYYGDFCTIHNAEFKYGARTYNDVQVGYNIEQINTRVDLGIDNVGDR
                     TPPLFYANNTLNANTDPGNFDVMGRYYWARVTVKF"
     gene            1527..>3193
                     /locus_tag="VFF05_07130"
     CDS             1527..>3193
                     /locus_tag="VFF05_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW51614.1"
                     /translation="MTANSQPIQTIVAAMRAGDPARAETLCRETLARTPDDTDIVFSL
                     AMSLHMQRKLEEAIPVYQRLTELQPGSAAHWSNYATALSNAGRAEEAEAAIRRALGLN
                     PDDANAHLQLGLLLVARHAYAAARDSLLDAFERERNSPAIRIHAANACSLCHDFQGAE
                     ELLHGWRQWLPLDDDTLQMELARLLLLLADAPGSRDLLNDIVRRQPGVAKPAIQLAMI
                     EERLNLLDDARTRLASLDRDALTLPQRIEFDFARATLAERSGNHAVARELLESAGPLN
                     DDDYAHYFALAKCCDKLGDGAATMQALAQAHARQTAQLKAVNPANYSAQASALPATVV
                     RTDAEQYRRWPVYRAPQAQDSPVFVVGFPRSGTTLLEQMLDAHPRLQSMDENPFFDRL
                     AGKLRAHDPRILDDLSVLRQYDCDELRKRYRVMAAAKVAIRADAQLVDKNPLNLLWLP
                     FIHRLFPNAKYILCLRHPCDVLLSCYMQNFRSGLLAAACENLPRLATAYVQAMRCWLD
                     HVEVFHPDVLVSRYEDLVADFEPQTRRIAEYLGLDDPAPMLGFDRRAR"
BASE COUNT          585 a         1108 c          933 g          567 t
ORIGIN      
        1 cctgaccaat gcttgggcgc aggaaaaggt cgcgcacctg gacttcaacg gtggtctgtt
       61 cgatctgcct gccggcaccg cgcagctcgc catcggcggc agttatcgca acgagtacac
      121 caacagcgtc gacgacgcga ccttgctgat caatccggat accggcaact gcacgctggg
      181 tagtcagtgc tcgtcgaacc tcaacggcgg atacaacgtc aaggaagcct acggcgagtt
      241 gttcctgccg ctgttcaagg acctgccgtt cgtcaattca ctgaacgtga cgctgagcga
      301 ccgctattcg aagtacaaca ccttcggcag caccaacaac gccgccatcg gcgtcgagtg
      361 gaagccgatc gacgacctgc tgctgcgcgc caactacaac aaggtgttcc gtgccccgac
      421 ggtgggcgac atcttcggca acccgatcag cgacgcgccg aaactcagtt ccgatccgtg
      481 caccggcatc accgtggcca acccggcctg cgtcggcgtg ccgcttgatg gatcgttcca
      541 gaataccgac gtgcagctcg gccagcagat caagggcatc gcttcgggct ctgcctatgc
      601 gggcttcccg ctcggccccg agaacggcgc gaccttcaac tggggcttcg tctactcgcc
      661 gcactgggtc gagggtctct cgctcagcgc cgacctgtgg cgcgtttacc tgaacaacac
      721 catcaccagt gttggtgccc aggccgtgct caacaactgc ttcgccggtc agctgcaata
      781 ttgcccgctg atcacgcgct tcggtcccga cagcacgcag ccgggtcagg tcaagcagat
      841 tctcgagccg accgccaacc tcggtcgcct ggataccaag ggcgccgact tctcggtcaa
      901 gtatcgcctg ccgcaattca gcttcggcca gttcgcggtc gctctggatg cgacctacct
      961 tgcccagtac aaccaggaat cgggtggcgt gctgtatcac gacgccggtc acctgctgaa
     1021 cttcggcagc ggcgcccaag ccgcgtgccc ggatctcggc ggcggcgtgt gcttgttccc
     1081 gcgttggcgc gcattggcgt cggtcaactg gaacctcggc tcgtgggatg cgtcatggcg
     1141 catgcgctat accggcaagt tccgcatggg ctcgaagtcg ccctcgcagg atacgttccc
     1201 ggtcggcacg tgctactacg gcgacttctg caccatccac aacgccgagt tcaagtacgg
     1261 cgcgcggacg tataacgatg tgcaggtcgg ctacaacatc gagcagatca acacgcgcgt
     1321 ggatcttggc atcgacaacg tcggcgacag gacgccgccg ctcttttacg ccaacaacac
     1381 gctcaacgcc aacaccgatc cgggcaactt cgacgtgatg ggccgctact actgggctcg
     1441 cgtgacggtg aagttctgat cgcgcagaaa gcgtaaagcg ttaccatccg agccgcgcga
     1501 gtctctcgcg cggctctttt tttgccatga ccgccaattc ccaacccatt cagaccatcg
     1561 tcgccgccat gcgcgccggc gatcccgcac gcgcggaaac cctctgccgc gaaaccctgg
     1621 cccgtacgcc ggacgacacc gacattgtgt tttcgctggc catgagcctg cacatgcagc
     1681 gcaagctcga ggaagccatt cccgtctacc agcgcttgac cgagctgcag cccggcagcg
     1741 ccgcgcactg gagcaactac gccaccgcgc tgtccaatgc gggccgcgcc gaagaagccg
     1801 aagcggccat ccgccgcgcg ctcgggctga atccggacga tgcgaatgcg cacctgcaac
     1861 tcggcctgct gctggttgcg cgtcacgcct acgcggcggc acgcgacagc ctgctcgacg
     1921 ccttcgagcg cgagcgcaac tcgccggcga tccgcatcca cgctgccaac gcctgctcgt
     1981 tatgccacga ttttcagggc gccgaagaac tgctccacgg ctggcggcag tggctgccgt
     2041 tagacgacga cacactgcaa atggaactcg cacgactcct gctgctgctg gcggatgcgc
     2101 cgggcagccg cgatctgctg aacgacatcg tcaggcgcca gcccggggtc gcgaaacctg
     2161 cgatccagct tgcaatgatc gaggaacggc tgaacctgct cgacgacgcc cgcacgcgac
     2221 ttgcgtcgct cgaccgtgat gcgctcactc tgccgcaacg catcgaattc gatttcgccc
     2281 gtgccacgct cgccgagcgc agtggcaatc acgccgtcgc gcgggaactg ctcgaatccg
     2341 ccgggccgct gaacgacgac gattacgccc actatttcgc gcttgccaaa tgctgcgaca
     2401 agctcggcga cggtgcggca accatgcagg cgcttgcgca ggcgcatgcg cgccagactg
     2461 cgcaattaaa agccgtgaac ccggcgaact actcggcaca agccagcgcg ctgcccgcca
     2521 cagtcgtgcg cacggacgcc gaacaatacc gccgctggcc ggtctatcgc gcgccgcagg
     2581 cgcaggattc gccggtcttc gttgtcggct ttccgcgctc cggcacgacc ctgctcgaac
     2641 agatgttgga cgcacacccg cgcctgcaat ccatggacga gaatcctttc ttcgaccgtt
     2701 tggccggcaa gctgcgcgcg cacgatccgc gcatcctcga cgacctttcc gtgctgcgcc
     2761 agtacgattg cgacgagttg cgcaagcgct atcgcgtcat ggccgcagca aaggtggcga
     2821 tccgcgcgga cgcgcaattg gtcgacaaga atccgctcaa cctgctctgg ctgccattca
     2881 tccatcgcct gttcccgaac gcgaagtaca tcctgtgcct gcggcatccc tgcgacgtgc
     2941 tgctgtcgtg ttacatgcag aacttccgct cgggtctgct cgcggctgca tgcgaaaacc
     3001 tgccgcggct ggccactgcc tacgtgcagg cgatgcgctg ctggctggat cacgtcgagg
     3061 tgtttcatcc cgacgtactg gtttcgcgtt acgaggatct ggtcgccgat ttcgaaccac
     3121 agacccggcg catcgccgaa tacctcggtc tggacgaccc cgcgcccatg ctcggattcg
     3181 atcggcgcgc gcg
//
LOCUS       DASRVO010000577         1114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_199005, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000577 DASRVO010000000
VERSION     DASRVO010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199005"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1114)
                     /locus_tag="VFF05_07135"
     CDS             complement(<1..>1114)
                     /locus_tag="VFF05_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HZW51615.1"
                     /translation="SIETTSICPGCGTRSGRVHSRYHRRLADLPIAGHPVRLMMRARR
                     FYCRAVLCGRHVFAERFDDDVLAPWARRTARLDHIVHHLGLALGGRPAASFARRLMVP
                     VSKDTLLRVIRRRGSPPFVPPTVIGIDDWAWRRNQRYGTLICDLERRRTIALLPDREP
                     ATARDWLSQQPQIAVVARDRGGAYAPAAAKALPDATQVADRWHLMENASRAFLDAVRR
                     SMRQIRSVIGAATINPSLLTAAERIQYEGYLRREEDNAAILRLAKDGIAIKEIVRRTG
                     YSRGLVRRILRGQRSDIFRVRESSLELYLPWLDERWAAGDRNAAALWRQLKQKGFRGC
                     MRVISEWATRRRQTDRIDSASSRTPAARTIARLMTIS"
BASE COUNT          208 a          374 c          346 g          186 t
ORIGIN      
        1 actgatggtc atcaaccgcg caatggtccg tgccgcaggc gttcggctcg acgcgctatc
       61 tattctgtct gtctggcgcc ggcgcgtggc ccattcgcta atgacgcgca tgcagccgcg
      121 gaaacccttc tgcttcagct gtcgccaaag tgcagcagcg ttgcgatcac cagcggccca
      181 gcgctcgtcg agccacggca gatagagctc gagcgagctc tcccgcacgc ggaagatgtc
      241 ggagcgctgt ccacgcaaga tccgccggac gaggccgcgg ctatatccgg ttcggcgcac
      301 gatctccttg atcgcgatcc cgtccttggc caggcggaga atggcggcgt tatcttcctc
      361 tcggcggaga taaccttcgt attggatccg ttcggcagcg gtgagcaggc tggggttgat
      421 cgtggccgcg ccgatcacag agcggatctg acgcatcgac ctgcgcaccg cgtcgagaaa
      481 ggcgcggctc gcattttcca ttaggtgcca gcggtcggcg acctgcgtcg catcgggcaa
      541 cgctttggcc gcggcaggcg cgtatgcccc gccacgatcg cgcgcaacca ctgcgatctg
      601 cggctgctgc gacagccaat cccgtgcagt cgcgggctca cgatcaggca gaagcgcgat
      661 cgtccgccgc cgctccaaat cacagatcaa cgttccgtag cgctgattgc gccgccatgc
      721 ccagtcgtcg atcccgatta cggtcggcgg cacaaaaggc ggactgccgc gtcttcggat
      781 aacccgcagc aacgtatcct tgctgaccgg caccatgagc cgacgcgcaa agctggccgc
      841 cggccgcccg ccaagtgcca gtccaagatg atggacgata tgatccagac gcgccgttcg
      901 acgcgcccac ggggcaagca cgtcatcgtc gaagcgctcg gcgaatacgt gccgcccgca
      961 tagaacggcc cgacaataga accgacgagc tcgcatcatt aggcgaaccg gatgcccggc
     1021 gatcggcaag tcagccaagc gccgatgata ccggctatgg acccggccgg atcgcgtccc
     1081 gcacccagga cagatgcttg tggtctcgat ggag
//
LOCUS       DASRVO010000578         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_277265, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000578 DASRVO010000000
VERSION     DASRVO010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277265"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFF05_07140"
     CDS             complement(<1..511)
                     /locus_tag="VFF05_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51616.1"
                     /translation="MSALFERLASGLPERHVPRRNSFASDAKSLRQWLAQLPLANPGA
                     TARLLISALREMNQLRIDPQQRIDALEMLREPIGNIVAVLGKQVLGDSFPLPPQKQQL
                     GQLAQDFEHELALGYCAAIYDLCAPAGAVPFLRGKSVALALTRALQHRGGSLYQAYLR
                     YHAPAPGAWQ"
     gene            complement(566..>2063)
                     /locus_tag="VFF05_07145"
     CDS             complement(566..>2063)
                     /locus_tag="VFF05_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HZW51617.1"
                     /translation="GINVIVQLIATRGQGALRRYSLACNPDVTADLLERMAAVGAARP
                     LIVGVIHPDLPFVGNEAEIDAAFFDIVLDTPESAHTLFALPRSPVDTAEYALGLHAST
                     LVRDGGTLQIGIGVLSDALVHALLLRHQQNADYRAVLYALGNVDAELCARRGGAEHFA
                     RGLYGASEMVMDGFMQLARAGILSRRVFDDLDVEKALATGRDVDVSGRHDGRYLRGAF
                     YLGSKDFYAWLRGLQGADFDGLSMTRVSDINQLYGGRETLDALQRRDARFFNTCMMAT
                     LLGAAVSDTLENGQVVSGVGGQYNFVAMAHALEGGRSILLLRATRRSGGKLRSNIVWN
                     YGQATIPRHLRDIYVTEYGVADLRGKSDEECIVAMLGIADARFIDALAAQAKAAGKLR
                     RDFVVPDAWRLNTPAHLGAALAPHHERFPHFPFGSDFDDTELRLLPALQRLQALSANK
                     ARLAVFALFAGRPRAGDMPLLERMGLAHPRGLAGRLLRRVLARALHDD"
BASE COUNT          383 a          679 c          667 g          334 t
ORIGIN      
        1 tctgccaggc gcccggcgcc ggtgcgtggt agcgcagata ggcctgatac aaacttccgc
       61 cgcgatgctg gagggcccgc gtcaacgcca gggcaacaga tttgccgcgc aggaacggca
      121 ccgctcctgc gggtgcgcac aggtcgtaaa tcgccgcgca ataacccaac gccagctcat
      181 gctcgaaatc ctgtgcgagc tgaccgagtt gctgcttctg tggcggcaac ggaaaactgt
      241 cgcccagcac ctgcttgccc agcacggcaa cgatgttgcc gatcggttcg cgcagcattt
      301 ccagcgcgtc gatgcgctgc tgcggatcga tgcgcaactg gttcatctcg cgcagcgctg
      361 aaatcagcaa gcgtgcggtc gcacccggat tcgccagcgg cagctgcgcc agccattggc
      421 gcagggattt ggcgtcgctg gcgaacgaat tgcggcgtgg aacgtggcgt tccggcagcc
      481 cgcttgccaa tcgctcgaac aacgcactca ttgccgaccc ctgtacatcg gccgcaagta
      541 tactccgaac gcgcgcggcc cccgctcaat cgtcgtgcaa ggctcgcgcc agcacgcggc
      601 gcaacaggcg tccggcaagg ccgcgtggat gcgccagacc cattcgttcg agcaagggca
      661 tgtccccggc gcgcggcctt ccggcgaaca gcgcgaaaac cgccaggcgc gctttgttcg
      721 ccgacaacgc ctgcaagcgc tgcaaggccg gcagcaggcg caattcggta tcgtcgaaat
      781 cgctgccgaa cggaaagtgc ggaaagcgtt catggtgcgg ggccagcgcc gcgccaagat
      841 gtgccggcgt gttgagacgc caggcatcgg gtacgacgaa gtcccgccgt agttttcctg
      901 cggctttcgc ctgcgcggcc agcgcgtcga tgaagcgcgc atcggcgatg ccgagcatgg
      961 cgacgatgca ttcctcgtcg gatttgccac gcaggtcggc gacgccgtat tcggtcacat
     1021 agatatcgcg caggtgacgc ggaatcgtgg cctggccgta attccagacg atgttcgagc
     1081 gcagtttgcc gccgctgcgg cgggtggcgc gcaacagcag gatcgaacgt ccgccctcga
     1141 gcgcgtgcgc catcgcaacg aagttgtact gtccgccgac gccgctgacg acctggccgt
     1201 tttccagcgt gtcggacacc gccgccccga gcagcgtagc catcatgcag gtattgaaga
     1261 aacgtgcgtc gcggcgttgc aacgcgtcga gcgtttcgcg tccgccgtaa agttgattga
     1321 tatcggacac gcgtgtcatc gacaggccgt cgaaatccgc gccctgcaat ccgcgcagcc
     1381 aggcgtagaa atccttcgag ccgagataga acgcgccgcg caggtagcgc ccgtcgtggc
     1441 gtccggaaac atccacgtcg cggcccgtgg ccagcgcttt ttcgacatcg aggtcgtcga
     1501 aaacgcgacg cgaaagaatc cctgcgcgcg ccagttgcat gaatccgtcc atgaccattt
     1561 cgctggcgcc gtagaggccg cgcgcgaagt gctccgcgcc gccgcggcgc gcgcaaagtt
     1621 ccgcatcgac attgccaagc gcatagagca cggcgcgata gtccgcattt tgctggtggc
     1681 gcagcagcag cgcatgcacg agcgcgtcgg acagcacgcc gatgccgatc tgcaaggtgc
     1741 cgccatcgcg cacgagcgtg ctcgcgtgca ggccgagcgc atattcggcc gtgtcgaccg
     1801 gcgagcgcgg caacgcgaac agcgtgtgcg ccgattcggg cgtatccagc acgatgtcga
     1861 aaaacgcggc atcgatttcc gcctcgttgc caacgaacgg caaatccgga tggatcacgc
     1921 ccacgatcag cggacgcgcc gcaccgaccg cggccatgcg ttcgagcagg tcggcggtga
     1981 cgtcgggatt gcaggcaagg ctgtagcggc gcagcgcacc ctgcccgcgc gtcgctatca
     2041 attgcacgat gacgttgatg ccg
//
LOCUS       DASRVO010000579         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_146022, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000579 DASRVO010000000
VERSION     DASRVO010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146022"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VFF05_07150"
     CDS             complement(<1..721)
                     /locus_tag="VFF05_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZW51618.1"
                     /translation="MSLPESLASALREIFGGALVVDPAECLAYGYDNSRRQAQPDAVV
                     FPIEHAQVVQLVRACRAARTPLIARGRGTNTTGATVPIGGGVVVSFERMNHIVRIDTD
                     NRLAVVEPGVLNGDLQAALAERGFFWPPDPTSSPYCTIGGNLACNAAGPRAVKYGSCR
                     ENTLALRAVTGAGEELRCGVNTTKGAVGYDLTRLLIGAEGTLAVITQATLKLTPKPAA
                     KATLAASYADVAAATAAVARLM"
     gene            complement(753..2138)
                     /locus_tag="VFF05_07155"
     CDS             complement(753..2138)
                     /locus_tag="VFF05_07155"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZW51619.1"
                     /translation="MLRPLSQAVALALAAGGAHAAAITVTDGGDAGTAGTCTLRQAIV
                     SANADSAQGTCTAGSGTDTITFAAGLANSTIALQYGQLLVNSSVTISGSGQTIDANGN
                     SRVMEISSANTVASVTVSDLTLTGGSYNGPGAGLSIKYGGPTAPNSRPQSGALAPAAA
                     GPNVTLNHVTISNNTSNLLAGGLFISNATVAINQSSISGNMLNASSNYGAGGVYIADG
                     STVTITDSTISGNSTSGSHAFLTGGVYVFGSALTVTNSTVTGNGASGGIDLAGGISLA
                     NSSAGIYNSTVTGNTTTGSNVSAGGILVGAEASASLNIFNSILANNTGTQANMGVINS
                     NVVAQSNLMGTTLSGLFPGNGNVFSDAPGLGALANNGGPTLTMLPQPGSPALGAGNAA
                     LTPAGVTTDQRGAGFPRVANGVLDIGAVQATAAAPIAAPTPTPALSRWAMLALAGLLG
                     LFGLRKRRHTS"
     gene            complement(2244..>2508)
                     /locus_tag="VFF05_07160"
     CDS             complement(2244..>2508)
                     /locus_tag="VFF05_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZW51620.1"
                     /translation="DQIEMTDAPEGFWRRRADETTTARVKTGLLDITSLPGFDPTRIN
                     VTTSHRVVYLMGLVDHAEADAATDVARNAQGVDKVVKLFDYVD"
BASE COUNT          399 a          781 c          875 g          453 t
ORIGIN      
        1 ccatcaggcg cgcgaccgcg gccgttgcgg cggcgacatc ggcgtagctc gcggcgagcg
       61 tggccttggc cgcgggtttt ggcgtgagct tgagcgtggc ctgcgtgatg acggcgagcg
      121 tgccttccgc gccgatcagc agccgcgtca agtcgtaacc gaccgcgcct ttggtggtgt
      181 tgaccccgca gcgcagctct tcaccggcgc cggttaccgc acgcagcgcg agcgtgttct
      241 cgcggcaact tccgtatttc accgcgcgcg gtccggcagc gttgcaggcg aggttgccgc
      301 cgatggtgca gtatgggctt gaagtcggat cgggcggcca gaagaatccg cgttcggcga
      361 gcgcggcttg caggtcgccg ttgagcacgc ccggttcgac cacggcgagg cgattgtcgg
      421 tgtcgatgcg cacgatatga ttcatgcgct cgaacgagac caccacgcca cccccgatcg
      481 gcaccgtcgc gccggtggtg ttggtgccgc ggccgcgcgc gatcagcggc gtgcgcgcag
      541 cgcggcaggc gcgcacgagt tgcacgacct gcgcgtgttc gatcggaaag acaacggcgt
      601 cgggctgggc ctgtcggcgc gaattgtcgt agccgtaggc gaggcattcg gcgggatcga
      661 cgaccagcgc gccgccgaaa atctcgcgca gcgcggaggc cagcgattcg ggaagactca
      721 tgccgccagt ttacgccggc gcggcgtgcg aatcagctcg tgtgtctgcg cttgcgcagc
      781 ccgaacaggc caagcaatcc ggcgagggcg agcatggccc agcgcgagag tgcaggcgtc
      841 ggcgtcggcg ccgcaatcgg tgcggcggcg gttgcctgca ccgcgccgat gtcgagtacg
      901 ccgttggcca cgcgcgggaa tcccgcgccg cgttgatccg tcgtcacgcc ggcaggagtc
      961 agggcggcat tgcccgcgcc caatgccgga ctgcccggtt gcggcagcat ggtcagcgtc
     1021 ggcccgccat tgttggccag cgcgccgagt ccgggcgcat cgctgaacac gttgccgttg
     1081 cctgggaaga ggccggaaag cgtcgtaccc atcaggttgg attgcgcaac gacattggaa
     1141 ttgattacgc ccatgttcgc ctgcgtgccg gtattgttgg cgagaatgga attgaagatg
     1201 ttgagactcg cactcgcctc tgcgcccacc agtattccgc ctgcggacac gttggagccg
     1261 gtcgtcgtgt tgccggtaac cgtgctgttg taaatgccag ccgaggaatt cgcgagcgaa
     1321 atgccgccag ccaagtcgat gccgccgctg gcgccgttgc cggtgacggt agagtttgtc
     1381 accgtaaggg cactgccaaa aacgtagacg ccgccggtca ggaacgcgtg gctcccggat
     1441 gtgctgttgc cggatatcgt cgaatcggta atggtgactg tcgagccgtc ggcaatgtaa
     1501 acgccgccgg cgccgtaatt gctcgaagca ttcagcatat tgcccgaaat gctggactgg
     1561 ttgatggcaa cggttgcgtt ggaaatgaat aacccgccgg ccaggagatt ggaagtgttg
     1621 ttgctgatgg tgacgtgatt cagggtgaca tttgggccgg ctgcggccgg cgcgagggcg
     1681 ccgctctgtg gtcgcgagtt cggagccgtc ggaccaccgt atttgatcga caggccggcg
     1741 cccggcccgt tgtagctgcc gccggtcagg gtcaggtcgg acaccgtcac gctcgcgacg
     1801 gtgttggcgc tggagatttc catcacccgc gaattgccgt tggcatcgat cgtctgcccg
     1861 ctgccgctga tggtcacgct actgttgacc agcaactgtc cgtattgcag cgcgatggtc
     1921 gaattcgcca aacccgccgc gaaggtgatc gtgtcggtgc cactgccggc cgtgcaggtg
     1981 ccctgggcgc tgtcggcgtt cgccgaaacg atggcctggc gcagcgtgca ggtgccggcc
     2041 gtgccggcat cgccgccatc ggtgacggtg atcgcggccg catgcgcacc gccggcggca
     2101 agcgccagcg ccacggcctg ggacaaggga cgcagcaact tggattccac ggaacacccc
     2161 tgatcggaca aagactgtgc gagtctactc ccgatccggg cgggctgtga aggacgtcac
     2221 gttcagtccg accgcattcg cgctcagtcg acgtagtcga acaacttcac gaccttgtcc
     2281 acgccttgcg cattgcgcgc cacgtcggtg gcggcgtcgg cctcggcgtg atcgaccaat
     2341 cccatcaggt agaccacgcg atgcgaggtc gtgacgttga tccgggtcgg atcgaagccg
     2401 ggcaggctgg tgatgtcgag caggccggtc ttgacccgtg cggtggtggt ctcatccgcg
     2461 cgccggcgcc agaatccttc cggtgcgtcc gtcatctcga tctggtcc
//
LOCUS       DASRVO010000580         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_171693, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000580 DASRVO010000000
VERSION     DASRVO010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171693"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1033
                     /locus_tag="VFF05_07165"
     CDS             83..1033
                     /locus_tag="VFF05_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51621.1"
                     /translation="MLLEWVNRARSDPQADLAGCSSATCAEKACYTTASPPRYLDLNL
                     SHSSRFHTNEMLVNDYFSHPSQCTLHSTIGSLYPTSCGGAASCACTQGQLTTDPSTWT
                     DPFLRMGLFNVDSSNGQSEIIAGESPDPGPNAMFYIWQYEPTNDSTCGFHTNDDNGHR
                     FIIQYNGYGQLAGGGYAANTTGYATMDFSGTASTKPKIPSGSHYPRQAASVDAWVNWY
                     DTAGPNVHKIDVDGVCTDMTLTRGTQANGAWHLSLSGYGSGCHRYAFAFKDSAGKEVI
                     YPTSGALTIGNGGAQCPDYSLSPPPGCAGFDRIFANGFEP"
     gene            complement(1034..1954)
                     /gene="rocF"
                     /locus_tag="VFF05_07170"
     CDS             complement(1034..1954)
                     /gene="rocF"
                     /locus_tag="VFF05_07170"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HZW51622.1"
                     /translation="MQHFAPVSLIGAPTDIGAGDRGASMGPEALRVAGIAEALIARGL
                     DVADRGNLFGPLNPWLPPSAGYRHLTQVVEWNREVMRAVGAELERKRLPILLGGDHSL
                     GIGSITAVANHCRKEGKTLRVLWLDAHADFNTSQITPSGNIHGMPVACLCGLGPRELT
                     QLGGAAPAVDPACIRQVGIRSVDAGEKRLVHDQGLAVFDMRYIDEVGMRRTMEAALAD
                     MDKDTHLHVSFDVDFLDPDIAPGVGTTVPGGPNYREAQLVMEMIADTGRLGSLDIVEL
                     NPALDERNQTGKLAVDLVESLFGKSTLMRE"
     gene            2112..2699
                     /locus_tag="VFF05_07175"
     CDS             2112..2699
                     /locus_tag="VFF05_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004149267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HZW51623.1"
                     /translation="MSGLVDNVLANLNSDQIGAIASRLGVDPAQAQAAIEHAVPLIVG
                     GMAQNASSPAGANALNNALGAHGGDLSGILGSVLGGDAGSGIGGAILGHIFGGNQGAA
                     NQGLGQATGLGTQDAGQLMAILAPLVMGALANHAQGQGVGAGGLGGMLTQEVQQVQQQ
                     GGSAGGLLNSVLGNSGGLAGILQSAGGLLGAFTKR"
     gene            2752..>3041
                     /locus_tag="VFF05_07180"
     CDS             2752..>3041
                     /locus_tag="VFF05_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51624.1"
                     /translation="MRGILILATALLTLAPAARAATAIVNVGGAFNAFSPQTVTIDPG
                     DRITFVNKGGYHNAVADDGSFRCAQGCDGDGMGGSGNASNKNWTASVTFATPG"
BASE COUNT          568 a          969 c          986 g          518 t
ORIGIN      
        1 gctagtgttt ctgtgcgcaa tcgcgccgcg agcttttgca tatggcgagg ccgtgaacgg
       61 gttcccgaac tggaacgagc gcgtgctgct cgaatgggtc aatcgtgcgc gttccgatcc
      121 tcaggccgat ttagccggct gttcatctgc aacctgtgcg gaaaaggctt gctacacgac
      181 ggccagccca ccgcgctacc tggatctgaa tttatcgcat tcttcgcggt ttcatacgaa
      241 cgaaatgctg gtcaacgact atttttcaca tccctcccag tgcacgttgc actccacgat
      301 tggttcgctg tatccgacga gctgcggcgg cgcggcctcg tgtgcgtgca cacaagggca
      361 gttgacgacc gatccatcga catggactga tccatttttg cgtatgggcc ttttcaacgt
      421 cgacagcagc aacggccaga gcgaaatcat tgcgggggaa tcaccggacc ccggtccgaa
      481 cgccatgttt tatatctggc aatacgaacc gaccaacgac agcacgtgcg ggtttcacac
      541 gaacgacgac aacggtcatc ggttcatcat tcaatacaac ggatatggcc aacttgccgg
      601 tggcggatat gccgcaaaca cgacaggtta cgcgacgatg gatttcagcg gcacggcttc
      661 aaccaaaccc aaaatccctt ccggcagtca ttacccgcgg caagccgcca gcgtggatgc
      721 atgggtcaac tggtacgaca ccgccgggcc gaacgtgcac aagatcgatg tcgatggggt
      781 gtgcaccgac atgacgttga cgcgtggtac gcaagccaat ggcgcgtggc acctgagcct
      841 gagcggttat ggcagcggtt gccaccgcta cgctttcgcc ttcaaggaca gcgcgggcaa
      901 agaagtgatc tatccaacga gtggcgcgct gacgatcgga aatggcggtg cgcaatgccc
      961 ggactacagt ctttcgccgc cgccgggctg cgccggattc gaccggattt tcgccaatgg
     1021 cttcgagccg tagctactcc cgcataagcg tcgacttgcc gaacaggctt tcgacgagat
     1081 ccacggcgag cttgccggtc tggttgcgct cgtccagcgc ggggttgagt tcgacgatgt
     1141 cgagcgagcc caagcggccg gtgtcggcga tcatctccat gacgagctgc gcttcgcggt
     1201 aattcggccc gccgggcacg gtggtgccga cgccaggggc gatgtccggg tcgaggaaat
     1261 ccacgtcgaa gctgacatgc aggtgcgtgt ccttgtccat gtcggcgagc gcggcttcca
     1321 tcgtgcgacg catgccgacc tcgtcgatgt aacgcatgtc gaacacggcc aggccctggt
     1381 cgtggacgag gcgtttttcg ccggcgtcga cggaacggat gccgacctgg cggatgcagg
     1441 cgggatcgac ggccggcgcc gcaccgccga gctgcgtcaa ctcacgcggg ccgagtccgc
     1501 acaggcaggc caccggcatg ccgtggatgt tgcccgacgg cgtgatctgc gaggtattga
     1561 aatcggcgtg cgcatccagc cacagcacgc gcagcgtctt tccttccttg cggcaatggt
     1621 tggcgaccgc ggtaatcgag ccgatgccca gactgtggtc gccgccgagc aggatcggca
     1681 ggcgcttgcg ctcgagttcg gcgccaaccg cgcgcatgac ctcgcgattc cattccacga
     1741 cctgggtcag gtggcggtag ccggcgctcg gcggcagcca cgggttcagc ggtccgaaca
     1801 ggttgccgcg atcggcgacg tcgagaccgc gcgcgatcag cgcctcggca atgccggcca
     1861 cgcgcaacgc ctccggaccc atgctggcgc cgcggtcgcc ggcgccgatg tcggtgggcg
     1921 cgccgatcag ggaaacaggt gcaaaatgtt gcatagtata gttttccgca tgtcgtggcg
     1981 cgcaggcgcc tgcgcgtgcg cataggatag ccgtagcgtc gccaatgcgc acctgcaacc
     2041 gtcgtcgagg cgcgctacac tcgctcgggc aggcgcgaca cacccacccc agcgaacgaa
     2101 ggagcattcc catgagcgga ctcgtcgaca acgtacttgc caatctcaac agcgaccaga
     2161 tcggcgcgat cgcatccaga ctcggcgtcg atccggcaca ggcgcaggcg gccatcgagc
     2221 acgccgtgcc gctgatcgtc ggcggcatgg cccagaacgc gagctcgccc gccggcgcga
     2281 atgcgttgaa caacgcgctc ggcgcgcatg gcggcgatct gtccggcatt ctcggcagcg
     2341 tcctcggtgg cgacgcgggt tccggcatcg gcggcgccat cctcggccac atcttcggcg
     2401 gcaatcaggg cgccgccaac cagggcctgg gccaggccac gggtctcggc acgcaagacg
     2461 ccggccagct catggccatc ctcgcgccac tggtgatggg cgcactggcc aatcacgcgc
     2521 agggccaggg cgtgggtgcc ggcgggctcg gcggaatgct cactcaggaa gtccagcaag
     2581 tgcagcagca gggaggcagc gcgggcggcc tgctcaattc cgtgctgggc aacagcggtg
     2641 gactggccgg catcctgcaa agcgcgggcg gactgctcgg cgcgttcacc aagcgctgaa
     2701 agcatgcgcc aggtcacggc gcggcgatcg acgatcaggc atgatgacaa aatgcgggga
     2761 atcctgattc ttgccacggc gctgctcacg ctcgcgccgg cagcgcgtgc ggcgacggca
     2821 atcgtcaatg tgggcggcgc cttcaatgca ttttcgccgc agacggtcac gatcgatccc
     2881 ggcgacagga tcacgttcgt caacaagggc ggctatcaca atgccgtcgc cgacgatgga
     2941 tccttccgct gtgcacaagg ctgcgatggc gacggcatgg gcggcagcgg caatgccagc
     3001 aacaagaact ggacggcaag cgtcaccttc gccacgcccg g
//
LOCUS       DASRVO010000581         4518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_277350, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000581 DASRVO010000000
VERSION     DASRVO010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277350"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFF05_07185"
     CDS             <1..232
                     /locus_tag="VFF05_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW51625.1"
                     /translation="FRPIMMTSFAALMGTLPIALGLGAGAESRRPLGVAVVGGLLTSQ
                     VLTLYLTPVIYLWFDRVQRRFRRQGSAAPAVA"
     gene            288..680
                     /locus_tag="VFF05_07190"
     CDS             288..680
                     /locus_tag="VFF05_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HZW51626.1"
                     /translation="MDAAELEKICGAWPGASAVIRREDELVLSVAGRAFATLCLCGPE
                     RDRLSFAVEAGQFVELTEQPGIVPAHYAARPFWITVTEPERFAHAQIESFLRRSYELV
                     RACLSKRQQGALAASAPSPKRAPRKPRT"
     gene            682..1590
                     /locus_tag="VFF05_07195"
     CDS             682..1590
                     /locus_tag="VFF05_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZW51627.1"
                     /translation="MSAKADSLKTIFFALGANFVIFLTKLAAAFVTGSGSMLAEAVHS
                     LADCGNQGLLIAGMRHARRAASDDHPLGYGMVVYFWSFLVALLLFTVGGMFSIYEGIH
                     KLETHEPLRWPWLAVGVLAFGIVAESFSMWGCMREVNKARAGKSLWRWFRESRQSELI
                     VIFGEDLAALLGLSFALAAVLATMLSGNPVFDAAGSIAIGALLCVVAVFVAREVASLL
                     VGQSAEPGLRAELEVFVRAQPEVERLFNLITLQLGPDLMVATKAQMRGELTGAQLIDA
                     INRVEAAMKARFPEIRWSFFEPDVSD"
     gene            1587..2141
                     /locus_tag="VFF05_07200"
     CDS             1587..2141
                     /locus_tag="VFF05_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1453 domain-containing protein"
                     /protein_id="HZW51628.1"
                     /translation="MTSLPKQVLQMDPKLVTPFLFAAFVAFAIYRRVRRNIGRQRVQP
                     MRMRSRIILFGVVGALVLAVSARNVELFAAMAAGIAGGVALAWLGLRHTKFEITEQGS
                     FYTPHTYIGLFVSALLLGRIAYRFMIVYPAMQAAHQVNANPFAAYQKSPLTLAIFGIV
                     IGYYIAYYAGVLVSSAKPPAPANG"
     gene            complement(2148..2999)
                     /gene="rlmJ"
                     /locus_tag="VFF05_07205"
     CDS             complement(2148..2999)
                     /gene="rlmJ"
                     /locus_tag="VFF05_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HZW51629.1"
                     /translation="MNYRHAFHAGNFADVFKHAILTGLIESLKAKQTPFHYFDTHAGA
                     GRYDLRGDEARKTREHESGVQRLLEAVRLPALLHIYLNLVRALNAGINAHDVAIYLGS
                     PLIASLLMRETDHATLCELQAGETAALKKLFAGDARIAVHQRDGYAAMDALLPPPERR
                     GLVLIDPPFEAQEAEFDTIEAALAKALVRWPTGMYAIWYPIKLRQPTASFLRSFVRRK
                     IPKVLCAELLLHPDNSALRLNGCGMVIVNPPWKFDRQLAELMPALREHLAQGRFGQQR
                     VEWLTGE"
     gene            3115..3504
                     /locus_tag="VFF05_07210"
     CDS             3115..3504
                     /locus_tag="VFF05_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51630.1"
                     /translation="MKRKFFAGRRLRLRLAALAAFALLFQQFAFAAHFCAVSGSAAGS
                     DCMGMSAADMALMHGDAAALCAHHCASAAVTPSPDAHALDVPPLMLPVLPTATVVVVS
                     VATAPSSLRNLPHPSPPLRDLFCSRQI"
     gene            3574..>4518
                     /locus_tag="VFF05_07215"
     CDS             3574..>4518
                     /locus_tag="VFF05_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZW51631.1"
                     /translation="MVTRICNRRKHALLPLVAAAACASFAHAAQAQPLTMDAAVALAV
                     RNAPMLAADAARVDAAREDRMRAGRLPDPELGIGLDNLTATGGGAFDPLADSMTMQTI
                     GIVQRIPPRAARAAQRAAADAQVLAASAQRTLAGADLRQSTADAWVSLWAAQRRLGLL
                     EDLRGAADVAERASRSRLAGGTDGAADALAARAEAADLDNRIDAARAGVESAQAMLRR
                     WTGTDASAADVADFAELPVAPATLLSQLDRQAPLLQWPAREASAQASLDAARAGKRPQ
                     WSVGVSYGKRFGSLPDMASVNFGMTLPLFAGNRQDRDIG"
BASE COUNT          677 a         1479 c         1503 g          859 t
ORIGIN      
        1 cttccgtccg atcatgatga ccagcttcgc cgcgctcatg ggcaccctgc cgatcgcgct
       61 gggcctgggt gccggcgccg aatcgcggcg tccgctgggc gtggcggtgg tcggcggcct
      121 gcttacctcg caggtgctga ccttgtacct gacgccggtg atctacctgt ggttcgaccg
      181 cgtgcaacgg cgtttccgcc gccagggcag cgccgcgccc gcagtagcct gagtcacgcc
      241 ataccgtcga gttttgttaa agtggcggac ttgcgatggg tgtaggcgtg gacgcagcgg
      301 aactcgaaaa gatatgtggc gcgtggcccg gcgcgagcgc ggtgatccgg cgcgaggacg
      361 aactcgtgct gagcgtcgcg ggccgcgcgt ttgcgacgtt atgcctgtgc gggcccgagc
      421 gcgatcggct ttcgttcgcg gtcgaggcgg gacagttcgt cgagctgacc gagcagcccg
      481 gcatcgtgcc agcgcattac gcggcgcgtc cgttctggat caccgtgacc gaacccgaac
      541 gatttgcgca cgcacagatc gaaagtttcc tgcgccgttc ctacgaactc gtccgcgcct
      601 gtctgagcaa acgccagcaa ggggcactcg cggcatccgc accgtcgccc aaacgcgcgc
      661 cgcgcaaacc aagaacctga aatgtccgcc aaagcagact cgctcaagac catcttcttc
      721 gcgctcggcg cgaacttcgt cattttcctg accaagctcg ccgccgcttt cgtcaccggt
      781 tccggctcga tgctggccga agcggtgcat tcgctggccg attgcggcaa ccagggcttg
      841 ctgatcgcgg gcatgcgcca cgcgcggcgc gcggcatcgg acgaccatcc gctgggttac
      901 ggcatggtcg tgtatttctg gtcgttcctc gtcgccttgt tgctgttcac cgtcggcggc
      961 atgttctcga tctacgaagg tatccacaaa ctcgaaaccc acgaaccgct gcgttggccg
     1021 tggctggcag ttggggtgct cgcgttcggc atcgtcgccg aatcgttttc gatgtggggc
     1081 tgcatgcgcg aggtcaacaa ggcgcgcgcc ggcaaaagcc tgtggcgctg gttccgcgaa
     1141 agtcgccaga gcgaactgat cgtgatcttc ggcgaggatc tggcagccct gctcggcctt
     1201 tcctttgcct tggctgcggt gctggcgacc atgctttcgg gcaatccggt gttcgatgcc
     1261 gccggctcga tcgcgatcgg cgctctgctg tgcgtggttg cggtgttcgt cgcacgcgag
     1321 gtggcgtcgc tgctcgtcgg ccaaagcgcc gaaccgggat tgcgcgccga actcgaagtc
     1381 ttcgtgcgcg cgcagcccga agtcgagcgc ctgttcaacc tgatcacact gcaactcggc
     1441 cccgatctca tggtcgcgac caaggcgcag atgcgcggcg agcttaccgg tgcgcagctg
     1501 atcgacgcga tcaaccgcgt cgaagccgcg atgaaggcgc gctttcccga aattcgctgg
     1561 tcgttcttcg agccggatgt cagcgattga cctcgttgcc gaaacaggtg ctgcaaatgg
     1621 acccgaaact cgttacaccc ttccttttcg ccgcgttcgt ggcctttgcg atctaccggc
     1681 gcgtgcgccg caacattgga cgccagcgcg tgcagcccat gcgcatgcgc tcgcgcatca
     1741 tcctgttcgg cgtcgtcggc gcgctggttc tggccgtatc cgcgcgcaac gtcgaactgt
     1801 ttgctgccat ggccgcaggc attgccggcg gcgttgcgct ggcctggctg ggtttgcgcc
     1861 acaccaaatt cgagatcacg gagcagggca gcttttacac gccgcacacc tatatcggcc
     1921 tgttcgtctc ggcgttgttg cttggccgca tcgcgtaccg tttcatgatc gtctatccgg
     1981 ccatgcaggc cgcgcaccag gtcaacgcca atccattcgc tgcctatcag aagagtccgc
     2041 tgacgctggc gattttcggc atcgtgatcg gctactacat cgcctattac gccggtgtgc
     2101 tggtgagcag cgcgaagccg cctgctcctg cgaacggata gacgggatta ctcccccgtc
     2161 agccactcca cgcgctgctg gccgaagcgg ccctgggcga gatgctcgcg cagcgcaggc
     2221 atcagttcgg caagctggcg gtcgaatttc cacggcgggt tgacgatgac catgccgcag
     2281 ccgttcaggc gcagcgcgga attgtcggga tgcaacagca gttcggcgca caggactttc
     2341 gggatcttgc gccgcacgaa actgcgcagg aaggacgcag tcggttggcg cagtttgatc
     2401 ggataccaga tcgcatacat gccggtcggc cagcgcacga gcgccttcgc caatgccgcc
     2461 tcgatggtgt cgaattcggc ctcctgcgcc tcgaacggcg gatcgatcaa caccaggcca
     2521 cggcgttccg gcggcggcag aagcgcatcc atcgccgcgt agccgtcgcg ctggtgcacg
     2581 gcgatgcgtg cgtcgccggc gaagagcttt ttcaacgcgg cggtttcgcc cgcctgcaat
     2641 tcgcacaagg tggcgtggtc ggtttcgcgc atcaaaaggc tggcgatcag cggcgagccc
     2701 aggtagatcg cgacgtcgtg cgcattgatg ccagcgttca gcgcgcgcac caggttcagg
     2761 taaatgtgca gcagtgcggg caggcgcacc gcctcgagca ggcgctgcac gccagattcg
     2821 tgttcgcgcg tcttgcgcgc ctcgtcgccg cgcagatcgt agcgcccggc accggcgtgg
     2881 gtgtcgaaat agtggaacgg cgtctgcttg gccttgagcg attcgatgag gccggtcagg
     2941 attgcgtgct tgaacacgtc ggcgaagttg ccggcatgaa aggcgtggcg gtagttcatt
     3001 gacgacgtcg tttgctgcgc atgatcgctg tgcggaaagt gtagcaagcg gggcgcctac
     3061 cgcgctgggc ggacggcgag ggcgccacgc gcgcatttgc gctaaacttg ttgtatgaaa
     3121 cggaagtttt tcgcgggtcg acggttacgc ctgcgcctgg cggcgctggc agcgttcgcc
     3181 ctgctgttcc agcaatttgc gtttgccgcg catttttgtg ctgtctcggg ttcggccgcg
     3241 ggcagcgatt gcatgggtat gtcggcagcg gacatggcgt tgatgcacgg cgacgcggca
     3301 gcgctgtgcg cccaccactg cgccagcgcc gccgtcacgc cttcacccga tgcgcatgcg
     3361 cttgacgtgc cgccgctaat gctgccggtg ctgccgaccg ccactgtcgt cgttgtgtcc
     3421 gttgcgacgg cgccatcgtc gttgcgcaac ctgccgcatc cgtcgccgcc actgcgcgac
     3481 ctgttctgct cgcgccagat ctagaactcc accgctgatg tcgaggccgc cgtccttgcg
     3541 gatggcgcgt tttccgtcgc ttgtggagtt tgcatggtta cccgcatttg caaccggcgc
     3601 aagcatgcgt tgttgccgct tgtcgctgcc gccgcctgcg catcgttcgc gcatgccgca
     3661 caggcccaac ccctgaccat ggatgcggcg gtcgcgctgg ccgtgcgcaa cgcacccatg
     3721 cttgccgccg acgccgcgcg cgtggacgcc gcgcgcgagg atcgcatgcg cgccgggcgc
     3781 ctgccggatc ccgaacttgg catcggactg gacaatctca ccgccaccgg cggtggcgcc
     3841 ttcgatccac tggccgattc gatgacgatg cagacgatcg gcatcgtgca gcgtatcccg
     3901 ccgcgtgccg cgcgcgcggc gcagcgcgcc gctgcagacg cgcaagtgct agccgcctcc
     3961 gcgcaacgca cgctggccgg cgcggacctg cgccagtcca ctgccgatgc ctgggtttcc
     4021 ctgtgggccg cgcaacgacg gctcggcttg ctcgaggact tgcgtggtgc ggcggacgtg
     4081 gccgagcgcg cgagtcgatc tcggctcgcc ggcggtaccg atggtgccgc cgacgccctg
     4141 gcggcacgcg cggaagcggc cgatctcgac aaccgcatcg atgcggcgcg agcgggcgtc
     4201 gagtccgcgc aggcgatgct gcgacgctgg accggtaccg acgcgagcgc ggcggatgtg
     4261 gcggatttcg cggaattgcc ggtcgcaccc gcgaccttgt tgtcccaact ggaccggcag
     4321 gcgccgctgc tgcagtggcc ggcgcgcgag gcgagcgcgc aggcgtcgct ggatgcggcc
     4381 cgcgccggca aacgccctca gtggagcgtg ggcgtcagct atggcaagcg tttcggcagc
     4441 ctgcccgaca tggcttcggt gaacttcggc atgaccttgc cgctgttcgc cggcaatcgc
     4501 caggatcgcg acatcggc
//
LOCUS       DASRVO010000582         1400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_380097, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000582 DASRVO010000000
VERSION     DASRVO010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380097"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFF05_07220"
     CDS             complement(<1..349)
                     /locus_tag="VFF05_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZW51632.1"
                     /translation="MIGYVTLGTNDLPRAAAFYDVLLGVIGAKRIMENDQFIAWSTGH
                     DKPGLALTKPFNGKPATIGNGTMVAFGVASQDIVKALHAKALELGGTDEGAPGERFEG
                     FYAAYFRDPDGNKL"
     gene            complement(395..>1400)
                     /locus_tag="VFF05_07225"
     CDS             complement(395..>1400)
                     /locus_tag="VFF05_07225"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019338404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZW51633.1"
                     /translation="EAEQGMNVARIAVLLAGLPNGVPAVTVNRFCSSGLQAVAMAADR
                     IRLGEADLMLAGGTESMSMVPMMGYHPVFNPRVFDNEHIGIAYGMGITAEKVAEQWKV
                     TREEQDAFSVASHAKALAGITAGEFADEIAPYTLADRYPNLDKRGIVDDTRTVKIDEG
                     PRAGTTLEVLAKLKTVFRAGGSVTAGNSSQMSDGAGAVLLASEQAIKDYGLTPLARFV
                     GFSVAGVPPEIMGIGPKAAIPKACAQTGIKQDALDWIELNEAFAAQSLAVMKDLGLDP
                     AKVNPLGGAIALGHPLGATGAVRVATLLHGLKRRKLKYGMVTMCIGTGMGAAGIFEAV
                     "
BASE COUNT          228 a          446 c          458 g          268 t
ORIGIN      
        1 tgagcttgtt gccgtcggga tcgcggaaat acgcggcgta gaacccctcg aaacgctcgc
       61 ccggagcgcc ttcgtctgtc ccgccgagtt cgagcgcctt cgcgtgcagt gccttgacga
      121 tgtcctggct ggctacgccg aatgcgacca tcgtgccgtt gccgatggtt gccggcttgc
      181 cgttgaacgg tttggtcagg gccagacccg gtttgtcgtg cccggtgctc caggcgatga
      241 actggtcgtt ttccatgatg cgctttgcgc caatgacacc gagcagcaca tcgtagaaag
      301 cggccgcgcg cggcaggtcg ttggtgccga gggtgacata gccaatcatg gatgactccg
      361 gtgtggtggt acgccggagt ctagcgcatc gaccctagac cgcctcgaaa atccccgccg
      421 cacccatgcc ggtgccgatg cacatggtca ccatgccgta cttgagcttg cgacgcttga
      481 gtccgtggag cagggtggcg acgcgcacgg cgccggtcgc accgagcgga tgaccgaggg
      541 cgatggcgcc gccgagcgga ttgaccttgg ccggatccag acccagatcc ttcatcaccg
      601 ccagcgactg cgcggcaaag gcttcgttga gttcgatcca gtccaatgca tcctgcttga
      661 tgccggtttg cgcgcaggct tttggaatcg cagccttggg accgatgccc atgatctcgg
      721 gcggcacgcc ggcgacggaa aaaccgacga agcgggcaag cggcgtgagt ccgtagtcct
      781 tgatcgcctg ttcgctggcg agcagcacgg cgcccgcgcc atcggacatc tgcgacgagt
      841 tgccagcggt gacgctgccg cctgcgcgga acacggtttt cagtttcgcc agaacttcca
      901 gcgtggtgcc ggcgcgcgga ccttcgtcga tcttgaccgt gcgcgtatcg tcgacgatgc
      961 cgcgcttgtc caggttcgga taacggtcgg ccagcgtgta gggcgcgatc tcgtcggcga
     1021 actcgcccgc ggtgattccg gccagcgcct tggcgtggct cgcgacggag aaggcgtcct
     1081 gttcctcgcg cgtcaccttc cattgctcgg cgaccttctc ggcagtgatg cccatgccgt
     1141 aggcgatgcc gatatgttcg ttgtcgaata cgcgtggatt gaagaccggg tggtagccca
     1201 tcatcggcac catgctcatg ctttcggtgc cgccggcaag catgaggtcg gcctcgccca
     1261 agcggatacg atcggctgcc attgcgacgg cctgcaggcc cgaggaacag aagcgattca
     1321 cggtgaccgc cggcacgccg ttcggcaagc cagccagcag aaccgcgatg cgcgccacgt
     1381 tcatgccttg ctcggcctcg
//
LOCUS       DASRVO010000583         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_121191, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000583 DASRVO010000000
VERSION     DASRVO010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121191"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1934
                     /gene="dnaK"
                     /locus_tag="VFF05_07230"
     CDS             18..1934
                     /gene="dnaK"
                     /locus_tag="VFF05_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZW51634.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGTTARVIENAEGDRTTPSIVAFKD
                     NEVLVGATAKRQAVTNPKNTFYAVKRLIGRKFTDAEVKKDLDLVPYKIAAHDNGDAWL
                     ATADGKNLSPQEISAKILMKMKKTAEDFLGEPVTEAVITVPAYFNDSQRQATKDAGRT
                     AGLEVKRIINEPTAAALAYGIDKKGGDRKIAVYDLGGGTFDISIIEIAEVDGEKQFEV
                     LATNGDTFLGGEDFDKRLIDYLVDEFRKESGVDLKNDSLALQRLKDGAERAKIELSSA
                     QQTEVNLPYITADASGPKHLNIKLTRAKLEALVGDLIEKTIAPCRTALNDAGLKVSDV
                     NEVILVGGQTRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGGVLGGTVKDVLLL
                     DVTPLSLGIETLGGVMTKLIEKNTTIPTKNTQVFSTAEDNQGAVTVHVLQGERERASA
                     NKSLAKFDLAGIEPAPRGMPQVEVTFDIDANGILHVSAKDKKTGKEQRIEIKAGSGLN
                     EEEIQRMVRDAEANKEDDRKFHELVGSRNKADQLVHATRAAIKEHGGKVPDQIGAIEA
                     ALSDLEKVMKGDDKDAIEAKCTALEQAAQSLAAAASAGHSGAGDAGAQAQGGSAPKED
                     VVDAEFTEVKDGKQ"
     gene            2077..2679
                     /locus_tag="VFF05_07235"
     CDS             2077..2679
                     /locus_tag="VFF05_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HZW51635.1"
                     /translation="MEKGRLEAFTDGVIAVVITIMVLELKPPHEATFAALLANWPVFV
                     SYVLSFVYVGIYWNNHHHLMHFVEHVNGKVMWANLHVLFWISLFPLTTAWLNEAHGAQ
                     GEFPASVPTLVYGVVLLMTALAWIPLANSLIRCNGGHAGTLARALGSGWKEKISPVVY
                     LAAIALAFFAPIVSCVLYAAVAIIWVVPDRRIERHVMPNP"
     gene            2696..3814
                     /gene="dnaJ"
                     /locus_tag="VFF05_07240"
     CDS             2696..3814
                     /gene="dnaJ"
                     /locus_tag="VFF05_07240"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZW51636.1"
                     /translation="MSKRDYYEVLGVERSVTEVELKKSFRRLAMKYHPDRCPDDPHAQ
                     EKFKEAKEAYEVLGDPDKRALYDRHGHAAFEQGMGGRGGAGFADMGDIFGDIFGDIFG
                     GGRARAQRRGADLRYILELDLEEAVFGAEKEIRVPTLAACEKCAGSGSTDGKLGTCAT
                     CQGHGRVRMQNGIFSIQQACPHCGGSGRKIANPCKTCHGEGRVEQEKKLAVKIPAGVD
                     SGDRIRLSGEGEAGPAGSPAGDLYVEVNVREHAIFQRERDDLFCEVPIRFPQAALGAE
                     LDVPTLAGEANIKIPAETQTGALFRLRGKGVRNVRSGHVGDLICRVVVETPVKLSKHQ
                     RELLQQFEASFGDDAGKHTPRANSWFDGVKRFVEKVTS"
     gene            3843..>4320
                     /locus_tag="VFF05_07245"
     CDS             3843..>4320
                     /locus_tag="VFF05_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZW51637.1"
                     /translation="MSDLVDVVVYGSSGRMGQAVVRAILESDEARLSCALVRPGSGLA
                     DEPLDRVFGTLPRVVDFSTALDPDVPCDVLVDFSGPHAFDAALALAVARRAAFVSGTT
                     GLRDEQFAALERAAQTIPVLWAANFSLGVAMLLRVAKRIGAALPDWDCEIVEMHHAR"
BASE COUNT          859 a         1325 c         1419 g          717 t
ORIGIN      
        1 ttccggagac tgaaaccatg ggcaagatca tcggcatcga cctgggcacc accaattcct
       61 gcgtcgcggt gatggaaggc acgaccgcgc gcgtgatcga gaacgcggaa ggcgaccgca
      121 ctacgccgtc catagtcgcg ttcaaggaca acgaggtcct ggtcggcgcc acggccaagc
      181 gccaagcggt gaccaatccg aagaacacgt tctacgcggt caagcgcctg atcgggcgca
      241 agttcaccga tgccgaggtc aagaaggacc tcgatctggt gccgtacaag atcgcggcgc
      301 acgacaatgg cgacgcgtgg ctcgcgactg ccgacggcaa gaatctctcg ccgcaggaaa
      361 tttcggcgaa gatcctgatg aagatgaaga agacggccga ggatttcctc ggcgagccgg
      421 ttaccgaggc ggtcatcacc gtgcccgcgt acttcaacga ttcgcagcgc caggcgacca
      481 aggacgctgg ccgcaccgcg ggcctcgagg tcaagcgcat catcaacgag ccgaccgcgg
      541 cggcgctcgc ctacggcatc gacaagaaag gcggcgaccg caagatcgcg gtctacgacc
      601 tcggcggcgg cacgttcgat atttccatca ttgaaatcgc cgaagtcgac ggcgagaagc
      661 agtttgaagt gctggcgacg aacggcgaca ctttcctcgg cggcgaagac ttcgacaagc
      721 gcctgatcga ttatctcgtc gacgaattcc gcaaggaatc cggcgtcgac ctgaagaacg
      781 attcgcttgc cttgcagcgc ctgaaggacg gcgccgagcg cgccaagatc gagctgtcga
      841 gcgcccagca gaccgaggtc aacctgcctt acatcacggc ggatgcgtct ggtccgaagc
      901 acctgaacat caagctcacg cgcgccaagc tcgaagcgct ggtcggcgac ctgatcgaaa
      961 agacgatcgc gccgtgccgc accgcactca atgacgcggg cctgaaagtt tccgacgtca
     1021 acgaggtgat tctcgtcggc ggccagacgc gcatgcccaa ggtgcaggaa gcggtgaagg
     1081 acttcttcgg caaggagccg cgcaaggacg tcaacccgga cgaggccgtc gccgtcggcg
     1141 cggcgatcca gggcggcgtg ctcggcggca ccgtcaagga cgtgctcctg cttgacgtca
     1201 cgccgttgtc gctcggcatc gagacgctgg gcggggtgat gaccaaactg atcgagaaga
     1261 acaccacgat cccgaccaag aacacgcagg tgttctcgac cgccgaggac aatcagggcg
     1321 cggtcaccgt gcatgtgctg cagggcgagc gcgaacgcgc cagcgcgaac aagtcgcttg
     1381 cgaaattcga tcttgcaggc attgagccag ctccgcgagg tatgccgcaa gtcgaggtca
     1441 cgttcgacat cgacgcgaac ggcatcctgc atgtctcggc caaggacaag aagaccggca
     1501 aggaacagcg catcgagatc aaggctggct ccggcttgaa cgaggaagaa atccagcgca
     1561 tggtgcgcga cgccgaggcg aacaaggaag acgaccgcaa attccacgaa ctcgtcggca
     1621 gccgcaacaa ggctgaccag ctcgtccacg ccacgcgcgc ggcaatcaag gagcatggtg
     1681 gcaaggtgcc ggaccagatc ggtgcgatcg aggcagcgtt gtccgacctc gagaaggtga
     1741 tgaaaggcga cgacaaggat gcgatcgaag ccaagtgcac ggcgctggaa caggccgcgc
     1801 agtcgctggc cgcggcggca agcgctggcc actccggcgc gggcgatgcg ggagcacagg
     1861 cgcaaggcgg ttccgcaccg aaggaagatg tggtcgacgc cgagttcacc gaggtcaagg
     1921 acggcaagca gtaattctgt gttatccatc gacgcttcgc gtcgacggat caactgccgc
     1981 cgttggagcc tcgctgggaa gcgcgaggct caacgccggc taaggctaat tggccgcccg
     2041 tgttgcgccg ggcggcttgg ttttttgtga gacgatatgg aaaaggggag actcgaagcc
     2101 ttcaccgacg gcgtcatcgc cgtggtcatc acgatcatgg tgctggaact caagccgcca
     2161 cacgaggcga cgttcgcggc gctgttggcc aactggccgg tgttcgtaag ctacgtgctc
     2221 agcttcgtct atgtcggcat ctactggaac aatcatcatc acctcatgca cttcgtcgag
     2281 cacgtcaacg gcaaggtgat gtgggcgaac ctgcacgtgc tgttctggat ttcgttgttt
     2341 cccctcacca ccgcatggct caacgaagcc cacggcgcgc agggcgaatt ccccgcgtcg
     2401 gtgccgacgc tggtctacgg cgtcgtactg ctgatgaccg cgctggcgtg gattccgctg
     2461 gcgaattcgc tcatccgatg caatggtggc cacgcgggaa cgcttgcgcg cgcgcttggc
     2521 agcggctgga aggaaaaaat ctcgcccgtc gtttacctcg cggctattgc gctggcgttt
     2581 ttcgcgccca ttgtttcctg cgtgctgtat gcggccgtgg cgatcatctg ggtcgtgccg
     2641 gatcgccgca tcgaacggca cgtgatgcca aacccgtgaa cctgtccgta tccccatgag
     2701 caagcgcgat tattacgaag tcctcggcgt cgaacgcagc gtcaccgagg tcgaactgaa
     2761 gaaatccttc cgccgtctgg cgatgaaata ccaccccgac cgctgcccgg acgatccgca
     2821 cgcgcaggaa aaattcaagg aagccaagga agcctacgaa gtccttggcg atccggacaa
     2881 gcgcgcgttg tacgaccgtc acggccatgc cgccttcgag cagggcatgg gcggacgtgg
     2941 cggcgccggc ttcgccgaca tgggcgatat ttttggcgac atcttcggcg atatcttcgg
     3001 cggcggacgc gcgcgtgcgc agcgacgcgg cgcggatttg cgctatatcc tcgaacttga
     3061 cctggaagag gctgtgttcg gcgcggagaa ggaaatccgc gtgccgacgc tcgcagcctg
     3121 cgagaaatgc gccggctccg gttccacgga cgggaaactc ggcacttgcg ccacctgcca
     3181 gggccacggc cgcgtgcgca tgcagaacgg gatcttctcg atccagcagg cctgtccgca
     3241 ttgcggcggc agcggcagga agatcgccaa tccatgcaag acctgccatg gcgaaggccg
     3301 tgtcgagcag gagaaaaaac tcgcggtgaa gattcccgcc ggcgtcgaca gcggcgatcg
     3361 cattcgcctg tccggcgaag gcgaggcggg accggcgggt tcgcctgcgg gcgacctgta
     3421 tgtggaagtg aacgtgcgcg aacacgcgat tttccaacgc gaacgcgacg atttgttctg
     3481 cgaggtaccg atccgcttcc cgcaagcggc gcttggcgcc gaactcgatg tgccgacgct
     3541 ggccggcgaa gcgaacatca agattcccgc ggagacccag accggcgcgt tgttccgcct
     3601 gcgcggcaag ggcgtgcgca acgtgcgcag cggccacgtc ggcgacctga tctgccgcgt
     3661 cgtggtggaa acgccggtga agctcagcaa gcaccagcgc gagctgctgc agcaattcga
     3721 ggccagcttt ggcgacgatg ccggcaagca caccccgcgc gcgaattcgt ggttcgacgg
     3781 cgttaagcgc ttcgtggaaa aggttacttc ctgaagcgtc gcgttgcggc taggatggcc
     3841 gcatgagcga tctcgtcgac gtcgtcgttt acggttcctc cggccgcatg ggccaggcgg
     3901 tggtcagggc gatcctggaa tccgacgagg cgcgcttgtc ctgtgcgctg gtgcgtccgg
     3961 ggtcagggct ggcggacgag ccactggatc gcgtcttcgg cacgctgccg cgcgtggtgg
     4021 atttctccac ggcactggat ccggacgttc cttgcgacgt gctggtggat ttcagcggtc
     4081 cgcatgcctt cgatgccgcg ctcgcgctgg cggtggcgcg ccgcgccgcc ttcgtcagcg
     4141 gcaccaccgg attgcgcgac gaacagttcg ccgcactcga acgcgcggcg caaacgatcc
     4201 cggttttatg ggcggcgaac ttcagcctcg gcgtggcgat gcttttgcgc gtagccaagc
     4261 gcatcggcgc ggcgttgccg gactgggatt gcgaaatcgt ggaaatgcat catgcgcgca
//
LOCUS       DASRVO010000584         1136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_354188, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000584 DASRVO010000000
VERSION     DASRVO010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354188"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..>1136)
                     /locus_tag="VFF05_07250"
     CDS             complement(138..>1136)
                     /locus_tag="VFF05_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZW51638.1"
                     /translation="IVAFYGGVLQKLWALPSVQAAGVAETVPMSGAGESTAIDIPGYR
                     RTDPGQPPFANYTIASAGYFSAVGTPLLRGREFTTSDTADSLPVAIVSRAMADRYWPR
                     QDVIGKQVRIPIDRDPKIIVGVVADVKHQSLREDVSPEMYVPYTQKPWPSMQTMEVAV
                     RTRTDPAALTASVRAAIQSLDPGLPLARVRTLTALVDDSMTQPRFALLLLASFAAIAL
                     LLAQIGMYGVVSYSVTQRTREIGIRMALGAPRQRVFAGVVAQAARLAAFGIAIGLLAA
                     LAVARLMAGFLYGVRPTSPLTFAAVSLLLVAVALLAAYLPARRASRLDPLIALRCE"
BASE COUNT          213 a          360 c          392 g          171 t
ORIGIN      
        1 ccagcatgag gttgccgcac acgttggcgg cgatgacgta cgccgtcagc agccgggttt
       61 tcgccgtttg cgtcatcctg cgcgacgagt gtaagcgaac accggctggt tgggatgcgg
      121 tcttgcggaa tgcaagctta ctcgcagcgc aatgcgatca acggatcgag gcgggaggcg
      181 cgccgggccg gcaggtaggc ggccagcagc gctactgcca ccaggagcaa gctcacggcc
      241 gcgaaggtga gcgggctggt gggacggacg ccgtagagaa atcccgccat caggcgcgcg
      301 accgccagag cagccagcag tccgatggcg attccgaatg ccgccaggcg agccgcctgc
      361 gccacaacgc cggcaaaaac gcgctgccgc ggcgctccca gcgccatacg aatgccgatt
      421 tcgcgggtgc gctgcgtcac cgaatacgag accacgccat acattccgat ttgcgccagc
      481 agcagcgcaa tggccgcaaa cgatgccagc agcagcagcg cgaagcgcgg ctgggtcatc
      541 gagtcgtcca ccagcgccgt aagcgtgcgc accctggcca gcggcaggcc gggatcaagc
      601 gactggatgg cggcgcgcac cgaagccgtc agcgccgcag gatcggtgcg cgttcgcacc
      661 gcgacttcca tggtctgcat cgaaggccac ggcttctgcg tgtacgggac gtacatttcg
      721 gggctgacat cttcccgtag tgactggtgc ttcacgtcgg ccaccacgcc gacgatgatc
      781 ttgggatcgc gatcaatggg aatgcgcacc tgtttgccga tcacatcctg tcgcggccag
      841 tagcggtccg ccatggcgcg gctgacgata gcgacgggca gggaatcggc cgtgtcggac
      901 gtggtgaact cgcgcccgcg cagcaggggc gtccccacgg cggagaaata tccggcagag
      961 gcgatggtgt agttggcgaa aggaggttgc ccaggatcgg tacggcggta gccgggaatg
     1021 tcaatcgcgg tgctctcccc ggcgccgctc atgggaaccg tttccgcgac gccggcggcc
     1081 tgcacgctgg gcagcgccca cagtttttgc aggacgccgc catagaaagc gacgat
//
LOCUS       DASRVO010000585         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_171962, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000585 DASRVO010000000
VERSION     DASRVO010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171962"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFF05_07255"
     CDS             <1..509
                     /locus_tag="VFF05_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HZW51639.1"
                     /translation="AIDALAAAARGAGLIALHTAADAREAETLWAARRALSPALRTIS
                     PDKINEDVVVPVSRLPELIATLRGIARKHAIPIVNFGHAGNGNIHVNLLPRDAHEHAR
                     AENALPEVFAAVLELGGTLSGEHGIGLSKRDFLTQALDPAALALMRGIKAQFDPDGIL
                     NPGKLLPP"
     gene            506..1087
                     /locus_tag="VFF05_07260"
     CDS             506..1087
                     /locus_tag="VFF05_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HZW51640.1"
                     /translation="MTKKLDSLLRDIRACRICEEHLPLGPNPVLRASASARILIVGQA
                     PGTKVHATGIPWNDASGERLRAWMDVDRDTFYDESRIAILPMGLCYPGRGRGGDNPPR
                     PECARTWHPQLIPLLRSVRLTLLIGQYAHRYFLRERRRDSLTATVRAFGDYLPQFLPM
                     VHPSPRNQGWLKRHAWFEKDLLPVLRERVHELL"
     gene            complement(1058..1561)
                     /locus_tag="VFF05_07265"
     CDS             complement(1058..1561)
                     /locus_tag="VFF05_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZW51641.1"
                     /translation="MSLDATQRRALLRHPAGWIACGFGSGLSPFAPGTAGSLAALLPW
                     LALREVPLPCFVAALVLAFALGVWACGWAVRTLKIADPSVVVWDEFVGQWIALAPLVW
                     WPRGWPWAICGFALFRIFDILKPWPVSWADRKVPGGLGVMLDDVIAGVYAALALAVLQ
                     QLVHAFA"
     gene            complement(1558..>2166)
                     /gene="thiL"
                     /locus_tag="VFF05_07270"
     CDS             complement(1558..>2166)
                     /gene="thiL"
                     /locus_tag="VFF05_07270"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZW51642.1"
                     /translation="ALVGGDTTQGPLSITVTIHGLVPDGAALARSGAHLGDGVYVTGT
                     LGDAAAGLRLLQSGQTQSAYLPGRLNRPTPRVEQGLILRGRASACIDVSDGLAADLGH
                     ICDASGVAAQIDADALPASSELAAAFDAPTRHEFQLAGGDDYELCFTAPDAVASDLLR
                     ELADSGCGATCIGRIVAGANVGVHDASGNPVSVPRRGWEHFA"
BASE COUNT          400 a          756 c          696 g          314 t
ORIGIN      
        1 atgccatcga cgcgttggcc gcggctgcgc gcggcgccgg actgatcgcg ctgcacaccg
       61 ccgccgatgc acgcgaagcc gaaactttgt gggccgcacg ccgcgcgctg tcgcccgcgc
      121 tgcgcacgat ctcgccggac aagatcaacg aggacgtggt cgtgccggtt tcgcgcctgc
      181 cggagctgat cgcgacactg cgcggcattg cgcgcaagca cgcaatcccc atcgtgaact
      241 tcggccatgc gggcaatggc aacatccatg tgaatttgct gccgcgcgac gcacacgagc
      301 atgcgcgcgc ggaaaacgcc ctgcccgagg tgttcgccgc ggtgctcgaa cttggcggca
      361 cgctgtccgg cgagcacggc attggcctgt ccaagcgcga tttcctcacc caggcgctcg
      421 atccagcggc actcgcgctg atgcgcggga tcaaggcgca gttcgatccg gacgggatac
      481 tcaatccggg aaagctgctg cccccgtgac gaaaaaactc gactcgctgc tgcgcgatat
      541 ccgcgcttgc cgcatctgcg aagagcatct gccactcgga cccaatcccg tcctgcgcgc
      601 aagcgccagc gcgcgaatcc tgattgtcgg gcaggcgccg ggcacgaagg tgcatgcaac
      661 aggaattcca tggaacgatg cgagcggcga acgcctgcgc gcgtggatgg acgtggaccg
      721 cgacactttt tacgacgaat cgcgcatcgc catcttgccg atggggttgt gttatcccgg
      781 ccgaggccgc ggcggcgaca atccaccgcg gccggaatgc gcacgcacct ggcatccgca
      841 attgattcca ctgttgcgca gcgtgcgcct gaccttgctg atcggccagt acgcgcaccg
      901 gtatttcctg cgcgagcggc gccgcgattc gctcaccgcg acggtgcgcg cgttcggcga
      961 ttacctgccg caattcctgc cgatggtgca tccgagtccg cgcaaccagg gctggctcaa
     1021 acgccatgcc tggttcgaga aggatctgct gccggtgtta cgcgaacgcg tgcacgagct
     1081 gctgtagcac cgccagcgcc agcgccgcgt agacgccggc gatcacgtca tcgagcatca
     1141 cgccgagccc gccgggaacc ttgcggtcag cccacgacac cggccagggt ttgaggatgt
     1201 cgaaaatacg gaatagcgcg aatccgcaga tggcccacgg ccatccacgc ggccaccaca
     1261 cgagcggcgc cagcgcgatc cactggccga cgaattcatc ccagaccacg acggacggat
     1321 cggcgatttt cagcgtgcgc acggcccagc cgcaggccca tacgccgagc gcgaacgcaa
     1381 gcacgagcgc agcgacgaag catggcagcg gcacctcgcg cagcgccagc catggcagca
     1441 gcgcggccag cgatccggca gtgcccggtg cgaagggact caaaccggaa ccgaagccgc
     1501 aggcgatcca accggccgga tgcctgagca gcgcgcggcg ttgtgtggca tccagactca
     1561 tgcaaaatgt tcccaaccac gtcgcggcac tgagacggga tttccggaag catcgtgcac
     1621 gcctacgttc gcgcccgcga cgatgcggcc gatgcacgtc gcgccgcagc cgctgtccgc
     1681 aagctcgcgc agcagatcgg atgcgaccgc gtccggcgca gtgaaacaca attcgtagtc
     1741 gtcgccaccg gcaagctgga attcatggcg cgtcggcgca tcgaacgcgg cggcaagctc
     1801 ggacgacgcc ggcagcgcat ccgcgtcgat ctgcgcagca acgccactgg cgtcgcaaat
     1861 gtgtcccaga tcggcggcaa gcccgtcgga gacatcgatg catgcgctgg cccgaccgcg
     1921 caggatcaat ccttgctcga cgcgcggcgt cggccggttc aggcgccccg gcagatacgc
     1981 gctttgcgtc tggcccgatt gcagcaggcg caatcccgct gccgcgtcgc cgagcgtgcc
     2041 ggtgacatac acgccatcac cgaggtgcgc accggaccgc gcgagcgccg cgccatccgg
     2101 cacgaggccg tgaatcgtca ccgtgatcga cagcggacct tgcgtcgtat cgccgccgac
     2161 gagagc
//
LOCUS       DASRVO010000586         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_250951, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000586 DASRVO010000000
VERSION     DASRVO010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250951"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..519
                     /locus_tag="VFF05_07275"
     CDS             55..519
                     /locus_tag="VFF05_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZW51643.1"
                     /translation="MADPTSREIRGLMIPVTGGRVLLPNATVAEVITYSIPEKIPDAP
                     DWLLGRLSWRGWRLPLFSFSMLSGAVAKEGYTNAKVAVLKALGGNAKMPFLAMLAQGF
                     PRLTTITPDLLIPTGDESAHPQGVHAQVLVRDDQAVIPNLGQIEALVAQAIP"
     gene            complement(536..1270)
                     /locus_tag="VFF05_07280"
     CDS             complement(536..1270)
                     /locus_tag="VFF05_07280"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZW51644.1"
                     /translation="MRIPRIHVDHDLRPGREVLLPDQAGEHVARVLRLERGAPLILFN
                     GDGYEFDAALASLAKHAVTAEVGQKRVVERESPLPLTLAQAIARGERMDWVLQKATEL
                     GIARIVPIITERTEVKLDAERAERRMAHWRGVIASACEQCGRTHLPDLEAPQAMDRWL
                     ATLGDAPATRLALLPEGDATLRQFPQMDNGAILAVGPEGGFTANDVALLTQAGFHGLR
                     LGPRILRTETAGVAALAALQALFGDF"
     gene            complement(1272..>2579)
                     /locus_tag="VFF05_07285"
     CDS             complement(1272..>2579)
                     /locus_tag="VFF05_07285"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HZW51645.1"
                     /translation="PCTQMRDHAGADALPMTPIVRGEGAWLVDANGSRYLDAVSSWWT
                     NIFGHANPRIAAAVKDQLDKLEHVIFAGFTHEPAIQLAEELLRVAPPGFSRVFFADNG
                     SSAVEVALKMSFHYWRNLGQSHKTRFIALTGSYHGETLGALSVTDVPLYRATYAPLLH
                     DPIFVASPDCYERDAGESWREHSLRKLDDMRATLEHHAQEVCAVIVEPLVQCAGGMRM
                     YDPTYLTGLRKLCDEFGVHFIADEIAVGFGRTGTLFACEQAGISPDFLCLSKGLTGGF
                     MPLSAVLTTSDVYDAFWAEYASGKAFLHSHSYTGNPLACRAALATLSIFRDEPVVERN
                     RALAAHMAQRLAPLADHPHVADVRQTGMIAAVELVRDKSKRAPFAASERRGLRVYRHG
                     LERGVLLRPLGDVVYFMPPYVVTPDEMDLMVGVAIEGIEKAVT"
BASE COUNT          459 a          825 c          846 g          449 t
ORIGIN      
        1 aagcaattgg cggacaaaat gattgccgaa tacggcaagg cgtaaggagc gaacatggcc
       61 gatccaacca gccgcgaaat ccgcggactc atgattccgg tgaccggcgg acgcgtgctg
      121 ctacccaacg ccacggtcgc cgaggtgatc acctattcga tcccggaaaa gatccccgat
      181 gcgccggact ggctgcttgg ccgcctgtcg tggcgcggct ggcgcctgcc gctgttctcg
      241 ttctcgatgc tgtccggtgc ggtggcgaag gaaggctata ccaacgccaa ggtcgccgtg
      301 ctcaaggcgc tgggcggcaa cgccaagatg ccgttcctgg ccatgcttgc gcagggtttt
      361 ccgcgcctga ccacgatcac gccggacctg ctcatcccga ccggcgacga atccgcgcat
      421 ccgcagggcg tgcatgcaca ggtgctggtc cgcgacgatc aggcggtgat cccgaacctg
      481 gggcagatcg aggcgctggt tgcgcaggcg attccatagc gaagctgctg cgctttcaaa
      541 aatccccgaa cagcgcctgc aacgccgcca acgcagcgac gccggccgtc tccgtgcgca
      601 ggatgcgcgg tcccaagcgc aggccgtgga atcccgcctg cgtcagcagg gcgacatcgt
      661 tggcggtgaa tccgccttcc gggccgaccg ccaggattgc gccattgtcc atttgtggaa
      721 attgccgcaa ggtggcgtcc ccttcgggca gcagcgccag acgtgttgcg ggcgcgtcgc
      781 cgagcgtcgc cagccagcga tccatggcct gcggcgcttc gagatccgga agatgggtgc
      841 ggccgcattg ctcgcaggcg ctggcgatga cgccgcgcca atgcgccatg cggcgttccg
      901 cgcgttcggc gtcgagtttc acttcggtac gctcggtgat gatcggcacg atgcgcgcga
      961 tgccgagctc ggtggccttt tgcaggaccc agtccatgcg ctcgccgcgc gcgatggcct
     1021 gcgcgagcgt gagcggcagc ggcgattcgc gctcgacgac gcgcttctgg ccgacttccg
     1081 cagtgacggc atgcttggcc agcgaggcga gtgcggcgtc gaattcgtag ccgtcgccgt
     1141 tgaacaagat cagcggcgcg ccgcgttcca ggcgcagcac gcgcgcgaca tgctcgccgg
     1201 cctggtcggg gagtagcact tcgcggccgg ggcgcaaatc gtggtcgacg tggattctcg
     1261 ggattcgcat ttcaagtcac tgccttctcg atgccttcga tcgccacgcc gaccatcaag
     1321 tccatttcat ccggcgtgac gacatacggt ggcatgaagt aaaccacatc gccgagcgga
     1381 cgcagcagca cgccgcgctc gagtccgtgc cggtacacgc gcaggccacg tctttcggat
     1441 gccgcgaaag gcgcgcgttt tgacttgtcg cgtaccagtt ccaccgccgc gatcatgccg
     1501 gtctggcgca cgtcggccac gtgcggatga tctgccagcg gcgcgaggcg ctgggccata
     1561 tgcgcggcca gcgcgcgatt gcgttcgact acgggttcgt cgcggaagat cgacagcgtc
     1621 gccaatgcgg cgcggcaggc gagcggattg ccggtgtagc tgtgcgaatg caggaacgcc
     1681 tttccgcttg catactctgc ccagaaggca tcgtagacgt cgcttgtcgt caacacggcc
     1741 gacagcggca tgaagccacc ggtgagtccc ttcgacaagc acagaaagtc aggagaaatc
     1801 ccggcttgct cgcaggcgaa caaggtgccg gtgcgaccga agccgaccgc gatctcgtcg
     1861 gcgatgaaat gtacgccgaa ttcgtcgcag agcttgcgca gaccggtcag gtacgtggga
     1921 tcgtacatgc gcatgccgcc cgcgcattgc accagcggct cgacgatgac ggcgcagact
     1981 tcctgcgcat ggtgctccag cgtggcgcgc atgtcgtcca atttgcgcag ggaatgttcg
     2041 cgccaggatt cacccgcatc gcgttcgtag caatccggcg aggcgacgaa aatcggatcg
     2101 tgcagcaatg gtgcatatgt cgcgcgatac aacggcacgt cggtcaccga cagcgcaccc
     2161 aatgtttcgc cgtgatagct gccggtcagc gcgataaaac gcgtcttgtg agactgccca
     2221 aggttgcgcc agtagtggaa gctcatcttc agcgccactt ccactgcgct ggaaccgttg
     2281 tcagcgaaaa acacgcggga gaatccgggc ggagccacgc gcagcaattc ttccgcaagc
     2341 tggatcgccg gttcgtgggt gaatccggca aagatgacgt gttcgagctt gtcgagctga
     2401 tctttaaccg ccgcggcgat gcgcggattg gcgtggccga agatgttcgt ccaccacgag
     2461 ctcaccgcgt cgaggtagcg actgccgttc gcgtcaacca gccaggcgcc ttcgccgcgc
     2521 acgatcggcg tcatcggcag ggcgtccgca cccgcgtggt cgcgcatctg cgtgcacgg
//
LOCUS       DASRVO010000587         1165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_277740, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000587 DASRVO010000000
VERSION     DASRVO010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277740"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..>1165)
                     /locus_tag="VFF05_07290"
     CDS             complement(5..>1165)
                     /locus_tag="VFF05_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51646.1"
                     /translation="RADVAHGIIGWLVREMTTESGGFYAALDADSEHEEGKFYVWSRD
                     EVRALLDSDEFAAAARHYGFDRPPNFEGKAWNPVVAAPFNDVAAALGIAINAAQARVS
                     SARAKLFAARALRVRPGLDDKILTAWNALAISGIARAVRTLHAPPLLGLAENALDNIY
                     NAAWRDGRLYAKASADAARFPGYLDDHALLLDALLEMLQCRWSERDIAWAIVLAEALL
                     ERFEDRERGGFFFTAHDHEKLIQRSKPFTDEALPSGNGVAARALLRLGHLVGETRYLD
                     AAERALRAGFANMRQMPQACCSMLRALNDFLHPRTHVVVRFDGDGEETKWRAALATAE
                     LRCSDAYFIPRDAGKLPGTLAAQSHVAGGVAYVCRGTQCEAPLHDPSGFKFD"
BASE COUNT          206 a          367 c          393 g          199 t
ORIGIN      
        1 ccactcaatc gaacttgaaa cctgacggat cgtgtaatgg cgcctcgcac tgcgtgccgc
       61 ggcagacgta agccacacct cccgcaacat ggctttgcgc ggccagtgtg ccgggcaact
      121 tgcccgcgtc gcgggggatg aaatacgcgt cgctgcagcg caattccgct gtggcgagtg
      181 cggcgcgcca tttcgtttcc tcgccatcgc cgtcgaagcg cacgacgacg tgggtacgcg
      241 gatgcaggaa atcgttcaac gcgcgcagca tgctgcaaca agcctgcggc atttgccgca
      301 tgttcgcgaa tccggcgcgt agcgcgcgtt cggcggcgtc gagataacgg gtctcgccga
      361 cgagatggcc caggcgcagc agcgcacgcg ccgcgacgcc gttgccggag ggtagtgctt
      421 cgtcggtgaa gggtttggac cgctggatca acttctcgtg atcgtgcgcg gtgaaaaaga
      481 atcccccgcg ttcgcgatcc tcgaagcgtt cgagcagggc ctcggcaagt acgatcgccc
      541 aggcaatgtc gcgctcggac cagcggcatt gcagcatttc gagcaatgca tcaagcaata
      601 gcgcgtggtc gtcgaggtag cccggaaagc gcgccgcgtc cgcgcttgcc ttggcgtaca
      661 agcgtccgtc gcgccacgcg gcattgtaaa tattgtccag cgcgttctcg gccaggccca
      721 gcaacggcgg cgcgtgcagc gtgcgaaccg cgcgtgcgat gccggaaatc gcaagcgcat
      781 tccaggcggt gaggatcttg tcgtcgaggc ctggacgcac gcgcaatgca cgcgcggcga
      841 acagtttcgc tcgcgcgctg gaaacgcgcg cctgcgccgc gttgattgca atgccgaggg
      901 ccgcggcaac gtcattgaac ggcgcggcga ccacggggtt ccaggccttg ccctcgaaat
      961 ttggcgggcg gtcgaagccg taatggcgtg cggcggcggc gaattcgtcg gagtcgagca
     1021 gggcacgtac ctcgtcgcgc gaccagacgt agaacttgcc ttcctcatgt tcggagtcgg
     1081 catcgagtgc ggcgtagaag cccccggatt ccgtggtcat ctcacgcacg agccagccga
     1141 tgatgccgtg cgcgacgtcg gcgcg
//
LOCUS       DASRVO010000588         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_329282, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000588 DASRVO010000000
VERSION     DASRVO010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329282"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1052
                     /locus_tag="VFF05_07295"
     CDS             <1..>1052
                     /locus_tag="VFF05_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HZW51647.1"
                     /translation="GIVLEEAIKRVLRLPVVGSKSFLVTIGDRSVGGLCARDPMVGPW
                     QVPVADCAVTLVDFDGYAGEAMAMGERTPLALLSAPAAARMAVGEALTNLMAAPVASL
                     DEIKLSANWMAAVNHPGEDVALFDAVKALSELCPQLRISIPVGKDSLSMQTVWQQDGQ
                     QQRTVAPVSPVVSAFARIADARRALTPQLVLDVGATELWLLDLGEGRNRLGGSVLAQV
                     FNRVGGLPPDFDDAGLFARFFAALKAAREQNLLLAYHDRGDGGVIATLIEMAFAAHCG
                     LDLELTGWADNTLSALFSEELGAVVQIRAADREVFLALLDEHGLKDLAHFAGNPHSRK
                     RVKLWLNDEAIARWPW"
BASE COUNT          157 a          344 c          363 g          188 t
ORIGIN      
        1 acggtatcgt gctcgaagaa gcgatcaaac gcgtgctgcg cctgcccgta gtcggcagca
       61 agtctttcct cgtcacgatc ggcgaccgca gcgtcggcgg cctgtgcgcg cgcgatccca
      121 tggtcgggcc gtggcaggtg ccggtggccg actgcgcggt gacgcttgtg gatttcgacg
      181 gctacgccgg cgaagcaatg gcgatgggcg agcgcacgcc gctggcgctg ctctcggcgc
      241 cggccgccgc gcgcatggcg gtgggcgagg cgctgaccaa cctcatggcc gcgccggtcg
      301 cgagcctgga cgagatcaag ctctcggcga actggatggc ggcggtcaac catcccggcg
      361 aagacgtcgc cctgttcgat gcggtgaaag cgctgtccga actttgtcca caattgcgca
      421 tttccattcc ggtcggcaag gattcgttgt ccatgcagac tgtgtggcag caggacggcc
      481 aacaacagcg cacggtcgcg cccgtttcgc ccgtcgtctc ggcgttcgcg cgcatcgccg
      541 acgcgcgtcg tgcgctgact ccgcaactcg tgctcgatgt cggcgcaacc gagttgtggc
      601 tgctcgacct gggcgaaggc cgcaatcgcc tcggtggctc ggtgctggcc caggtcttca
      661 accgagtcgg tggcctgccg ccggatttcg acgacgctgg attgttcgcg cgtttcttcg
      721 ccgcactcaa ggcggcgcgc gaacaaaacc tgctgctcgc ctaccacgat cgcggcgatg
      781 gcggcgtcat tgcgacgctg atcgagatgg ctttcgccgc gcattgcgga ctggacctgg
      841 aactgaccgg ctgggccgac aacacgctgt cggcgctgtt ctccgaggag ctcggcgcgg
      901 tggtgcagat cagggccgcc gaccgcgaag tgtttctcgc cttgctcgac gagcacgggc
      961 tcaaggacct ggcgcatttc gccggcaatc cgcactcgcg caagcgcgtg aagctgtggc
     1021 tcaacgacga ggcgatcgcg cgctggccgt gg
//
LOCUS       DASRVO010000589         1167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_69332, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000589 DASRVO010000000
VERSION     DASRVO010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69332"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFF05_07300"
     CDS             <1..207
                     /locus_tag="VFF05_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HZW51648.1"
                     /translation="GRRDEARRIFERILSVRNDVGLLAEGYDPDAKVFTGNFPQAYSH
                     VGLINTAVRLSADHGPAAPERAQP"
     gene            400..>1167
                     /locus_tag="VFF05_07305"
     CDS             400..>1167
                     /locus_tag="VFF05_07305"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZW51649.1"
                     /translation="MVGLEPLASELYARSGAAAYEWPRAEFATSLEQVVRKFLPDASA
                     EQAAEFARGLRVEEFALARACAAGSEKAWEEFLTRYRERLFDAARAIAKDDATGRELA
                     DSLYADLYGTSTRDGQRVSKLASYSGRGSLEGWLRTVLAQEYVNRYRSHRRTVSFEEQ
                     EEGGAQFEAAVAEAQPVADARLEQATDAALASVDAESRYILASYYLDGRRLAEIARTL
                     GVHESTISRKVEKATAGLRKKIVEELMARGMDWRQAQE"
BASE COUNT          215 a          346 c          432 g          174 t
ORIGIN      
        1 ggccgccggg acgaggcgcg ccggatcttc gagcgaatcc tgagcgttcg caacgatgtc
       61 gggttgctgg ccgagggata cgatccggat gcgaaggtat tcacgggcaa ctttccgcag
      121 gcgtattcgc acgtcgggct gatcaacacg gcggtgcgac tgtcggcaga ccacggtccc
      181 gcggcacccg agcgcgccca accgtaaccg caaaagaatt tctgcaagcc ggcacagctc
      241 cggcgttctg ttgaaaggga gtggtgtctc gatcccggag gagaacgggc gcgcagcatg
      301 gcgttgcgga aaaaatctat ggccccggcg cggttggtaa tctggtacga tgcgcgcgtc
      361 ttgagcatgg gcggggcaca agcttcgggc acgcgggcca tggttggcct ggaaccgctg
      421 gcgagcgaac tctacgcgcg cagcggcgcc gccgcctatg aatggccgcg cgccgagttt
      481 gccacgtccc tggagcaggt tgtccgcaag tttctgccgg acgcctccgc cgaacaggca
      541 gccgagttcg cgcgtggcct gagggtggaa gagtttgcgc tggcgcgcgc ctgtgccgcc
      601 gggtcggaaa aagcgtggga agagttcctg acgcgctatc gcgagcgtct gttcgatgcc
      661 gcgcgcgcga ttgccaagga cgacgccacc ggacgcgaac tggccgactc gctttacgcc
      721 gatctctacg gcaccagcac gcgcgacggg cagcgcgtct cgaagctggc gtcgtattcc
      781 ggacgcggct cgctcgaagg atggctgcgg acggtactgg cgcaggaata cgtcaaccgc
      841 taccggtcgc accgccggac ggtgagcttc gaggaacagg aggagggcgg ggcgcagttc
      901 gaggcggcgg tcgcggaagc gcagccggtt gcggacgcgc ggctcgaaca ggcgacggac
      961 gcggcgctgg caagcgtaga cgccgagagc cgctacatcc tggcgtcgta ttacctggac
     1021 gggcgcagac tggcggagat cgcgcgcacg ttgggcgtgc acgaatccac cataagccgc
     1081 aaagttgaga aggcgacggc agggctgcgg aaaaagatcg tcgaggagct gatggcgcgc
     1141 ggcatggact ggcggcaggc gcaggaa
//
LOCUS       DASRVO010000590         1963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_172184, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000590 DASRVO010000000
VERSION     DASRVO010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172184"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFF05_07310"
     CDS             complement(<1..512)
                     /locus_tag="VFF05_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZW51650.1"
                     /translation="MLNVAKPIWEPGKERVEAANINRFMRFVREQTGNEDLRRYAPLY
                     DFSIRQPAKFWKLVWEFCGIRASGDLDPVVVDGDRMPGATWFPNVRLNFAQNLLRHKD
                     DRIALIARNEWGHKREYSYAELHEEAGKLSHALRAAGVGMGDRVAGFMPNIPETVIAM
                     LATASLGATWS"
     gene            complement(543..1493)
                     /locus_tag="VFF05_07315"
     CDS             complement(543..1493)
                     /locus_tag="VFF05_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZW51651.1"
                     /translation="MSAKILIVAEHLDGKLNASTARCVTCAREIKPDAVDVLVLADAP
                     AAIAAEAAKIEGVSKVLTAANAANAHALAAMYAPQIAAVAKNGYTHVFAPSTTFGKDV
                     APRAAALLGVAQVSDIMKAVDARTFQRPIYAGNAIVTVQAPADAILVATVRTASYPAA
                     ANGANAAPVEALNLAVELPSHTRFVELAQGKSDRPDLQSAARVVSGGRGVGSKENFAI
                     IYKLADKLGAAVGASRAAVDAGYVPSDLQVGQTGKIIAPELYMAIGISGAIQHLTGIK
                     DAGTIVAINKDGEAPIFEVADIGLVGDLFKLIPELEAALG"
     gene            complement(1493..>1963)
                     /locus_tag="VFF05_07320"
     CDS             complement(1493..>1963)
                     /locus_tag="VFF05_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZW51652.1"
                     /translation="QPLTAARTLLKLVEKEQPQFVILGKQAIDDDCNQTGQMLAALWN
                     RPQATFASKVEVNGAVARVTREVDAGLETIEVDLPAVITTDLRLNEPRFIKLPDIMKA
                     KSKPLETIDFASLGVESGDHLKTTHYAPPPKRSRGVMVKDAAELVAALKTKGLL"
BASE COUNT          311 a          617 c          657 g          378 t
ORIGIN      
        1 gaccacgtgg cgccgagcga cgctgtcgcc agcatcgcaa ttacggtttc cgggatgttc
       61 ggcatgaagc cggcaacgcg atcgcccatg cctacgcccg ccgcgcgcaa ggcgtgtgaa
      121 agcttgccgg cttcttcatg cagttcggcg taggagtatt cgcgcttgtg gccccattcg
      181 ttgcgcgcga tcaaggcgat gcgatcgtcc ttgtggcgca acaggttctg cgcgaaattg
      241 aggcgtacat tcgggaacca cgtcgcaccg ggcatcctgt cgccgtcgac gacgacagga
      301 tccagatcgc cgctggcgcg gatgccgcag aattcccaca ccagcttcca gaacttcgcc
      361 ggctggcgga tcgagaaatc gtacaggggc gcgtagcgac ggaggtcctc gttgccagtc
      421 tgctcgcgca cgaagcgcat gaagcggttg atattggccg cttcgacgcg ttccttgcca
      481 ggttcccaga tcggtttggc cacgttcaac atgtggatgc ttccttggca atggctttca
      541 gcttacccca atgccgcttc cagctccgga atcaacttga acagatcgcc gacgaggccg
      601 atgtcggcga cctcgaagat cggcgcctcg ccatccttgt tgatcgcgac gatcgtgccg
      661 gcgtccttga tgccggtcag gtgctggatg gcgccggaga tgccgatcgc catgtacagt
      721 tccggcgcga tgatcttgcc ggtctggccg acctgcaggt cgctcggcac gtaaccggcg
      781 tcgaccgccg cgcgcgaggc gccgaccgcc gcgccgagtt tgtcggcgag cttgtagatg
      841 atcgcgaaat tttccttcga gccgacgccg cgcccgccgg agacgacgcg cgccgcgctt
      901 tgcaggtcgg gacggtcgct cttgccctgc gcgagttcga cgaagcgcgt gtgcgacgga
      961 agttcgacgg cgagattcag cgcctcgacc ggcgccgcat tcgcgccgtt cgccgccgcc
     1021 ggataagaag cggtgcgcac ggtcgcgacg aggatggcgt cggccggtgc ctgcacggtg
     1081 acgatggcat tgccggcgta gatcgggcgc tggaacgtgc gtgcatccac ggccttcatg
     1141 atgtcgctga cctgggcgac gccgagcagg gccgccgcgc gtggcgcgac gtccttgccg
     1201 aacgtggtcg atggcgcgaa cacgtgggtg taaccgtttt tcgcgacggc ggcgatttgc
     1261 ggtgcataca tggcggcaag cgcatgcgcg ttcgcggcat tcgccgcagt cagcactttc
     1321 gacacgccct cgattttcgc ggcttccgcg gcgatggcgg ccggcgcgtc ggccagcaca
     1381 agcacgtcga cggcgtccgg cttgatctcg cgcgcacagg tcacgcaacg cgcggtggaa
     1441 gcgttgagct tgccatcgag gtgttcggca acgatcagga tcttggcgga catcacagca
     1501 accccttggt cttcaacgcc gcgacgagtt cggcggcatc cttcaccatc acgccacggc
     1561 tgcgcttggg cggcggcgcg tagtgcgtgg tcttgaggtg atcgccggat tcgacaccca
     1621 gcgaagcgaa atcgatcgtc tccagcggtt tggacttggc cttcatgatg tccggtagct
     1681 tgatgaagcg cggctcgttc aagcgcaaat ccgtggtgat gacggcgggc aggtccactt
     1741 cgatggtttc aaggccggca tcgacttcgc gcgtgacgcg tgcgacggcg ccgttgactt
     1801 ccaccttcga ggcgaacgtg gcctgcgggc ggttccacag cgcggcgagc atctgtccgg
     1861 tctggttgca gtcgtcgtcg atggcctgct tgccgaggat gacgaactgc ggctgttcct
     1921 tctcgaccag cttgagcagc gtgcgcgccg cggtcagcgg ctg
//
LOCUS       DASRVO010000591         1825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_146368, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000591 DASRVO010000000
VERSION     DASRVO010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146368"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VFF05_07325"
     CDS             complement(<1..511)
                     /locus_tag="VFF05_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZW51653.1"
                     /translation="MKKRKKNRSSKIKDAQREAERFRRRALAGFILIALCLAALAARF
                     WYLQVDRHAEFAARSDSNRISVRSIPPTRGLIYDRNGVLLAENVAAFRLEVVPEQVKD
                     MGAMLAALGHVIALSDDDIERFTALRRSKRAFDSIPLRLKLSEDEIARFSTQRWRFPG
                     VDVVPYLTRA"
     gene            complement(508..999)
                     /gene="mreD"
                     /locus_tag="VFF05_07330"
     CDS             complement(508..999)
                     /gene="mreD"
                     /locus_tag="VFF05_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012444098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZW51654.1"
                     /translation="MNRLRANDWLFWGSVFACYLLQLMPLPQALLPFKPWWLALVLVY
                     WTLEAPEHVSLGRAFVLGLIGDVFVGDMLGEQALRLCIFCFILMRFRARLRFFPMWQQ
                     ALAVLALLVNDRIVLLAERAAAGLGGAPPSYWLAPLVGALAWPWVFLLLDDARARLRA
                     RES"
     gene            complement(996..>1825)
                     /gene="mreC"
                     /locus_tag="VFF05_07335"
     CDS             complement(996..>1825)
                     /gene="mreC"
                     /locus_tag="VFF05_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZW51655.1"
                     /translation="ALAIGLMVSDHRNGWLTRVRYAASVAIEPMYRLASLPSQLARDA
                     GDAVADRTRLNARNAELERELLLAQARLNRLQTVREQNERLQQLLDAQRSLDVGVQLA
                     RIIDVDTDPFRHRVVVDAGVRQGVAAGEAVLDAHGVMGQIVEVLPDTSTVMLITDPAH
                     ALPVKSERTGLRAIARGTGALDSLELQNVPISADIKPSDKLVTSGLGGHFPAGFPVGN
                     VRAIRNDPSGMFAVATVAPSAALDRSGEVLILHDLPDPVGPPAAASAEGPPASGVQR"
BASE COUNT          316 a          612 c          625 g          272 t
ORIGIN      
        1 aggcgcgggt caggtacggc accacgtcga cgccggggaa acgccagcgc tgcgtggaaa
       61 accgcgcgat ctcgtcctcg gacaatttca ggcgcagcgg aatgctgtcg aacgcgcgct
      121 tgctccgacg cagcgcggtg aaacgctcga tgtcgtcgtc cgacagcgcg atcacgtggc
      181 cgagcgcggc gagcatcgcg cccatgtcct tgacctgctc gggcaccact tcgaggcgga
      241 acgcggcgac gttttcggcc agcagcacgc cgttgcggtc gtagatcagg ccgcgcgtgg
      301 gcgggatgct gcgtaccgat atgcggttgc tgtccgagcg tgcggcgaat tcggcgtggc
      361 ggtcgacttg cagataccag aaacgcgcgg cgagtgcggc caggcacagc gcaatgagaa
      421 tgaagccggc gagcgcgcgg cggcggaagc gctccgcttc gcgctgcgcg tccttgatct
      481 tgctggatcg gttttttttg cgctttttca cgattcacgg gcacgcaggc gagcgcgcgc
      541 atcgtcgagc agcaggaata cccacggcca cgccagcgct ccgacgagcg gcgccagcca
      601 gtagcttggc ggcgcgccgc caagcccggc ggcggcgcgc tccgcgagca gcacgatgcg
      661 gtcgttgacg agcagggcga gcaccgcgag cgcctgctgc cacatcggga agaagcgcag
      721 gcgtgcgcgg aagcgcatca ggatgaagca gaaaatgcaa aggcgcaacg cctgctcgcc
      781 gagcatgtcg ccgacgaaca cgtcgccgat caggccgagc acgaacgcgc ggcccaggct
      841 cacgtgctcg ggcgcctcca gtgtccagta gacaagaacc aacgccagcc accagggctt
      901 gaacggcagc aaggcctgcg gcaacggcat caattgcagc aggtagcagg cgaacacgct
      961 gccccagaac agccagtcat tggcgcgcag ccgattcatc gttgcactcc cgatgcaggt
     1021 gggccttcgg ccgaggctgc tgcgggcgga ccgaccggat cgggcaggtc atgcaggatc
     1081 agcacctcgc cgctgcgatc cagcgccgcg ctcggcgcga ccgtcgccac cgcgaacatg
     1141 ccgctgggat cgttgcggat ggcacgcacg ttgccgaccg gaaacccggc cgggaaatgt
     1201 ccgcccaaac ccgaggtgac cagcttgtcg cttggcttga tgtcggcgct gatcgggaca
     1261 ttctgcaatt ccagcgaatc cagcgcgccg gtgccgcgcg cgatcgcgcg cagtccggtg
     1321 cgctcgctct tcaccggcaa tgcgtgtgca ggatcggtga tcagcatgac cgtggacgtg
     1381 tccggcagca cttccacgat ctggcccatc acgccgtgcg catcgagcac cgcctcgccc
     1441 gcggccacgc cctggcgcac gccggcatcc accaccacgc gatggcggaa cggatcggta
     1501 tccacgtcga tgatgcgtgc gagctgcacg ccgacatcga ggctgcgttg cgcatcgagc
     1561 aactgctgca ggcgttcgtt ctgctcgcgc acggtttgca ggcgattcag gcgcgcctgc
     1621 gcgagcagca gttcgcgttc gagttccgcg ttgcgcgcgt tcaggcgagt gcggtcggcc
     1681 accgcgtcgc cggcgtcgcg cgccagttgc gatggcaggg acgcgagccg atacatcggt
     1741 tcgatcgcca cgctcgcggc gtaacgcacg cgcgtgagcc agccgttgcg gtggtcgctg
     1801 accatcaggc cgatcgccag cgcca
//
LOCUS       DASRVO010000592         1053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_277920, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000592 DASRVO010000000
VERSION     DASRVO010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277920"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..805
                     /locus_tag="VFF05_07340"
     CDS             281..805
                     /locus_tag="VFF05_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51656.1"
                     /translation="MIGATLCALTAGASAQNGGPGAATAPAGDGPFRIGAQARGPLHR
                     LWENSLSVKQERVACLGGYIDHDVVYITRIEVLAPARADSADISAVASLRECSAPTWL
                     GTVHTHIARFHGLPYTIFSAPDRYVMTLWRERWKAPGVFCILYSDADANCELGNTVSG
                     HVQYAHEKGNNIGL"
     gene            complement(802..>1053)
                     /locus_tag="VFF05_07345"
     CDS             complement(802..>1053)
                     /locus_tag="VFF05_07345"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51657.1"
                     /translation="GAFSPGPSAALIDRMSEWGAAGVGQSSWGPAVYGIVDGDDAARQ
                     LADDVRNATSPGALFVGPFANTGARVWRDAPSDASPLSR"
BASE COUNT          187 a          339 c          356 g          171 t
ORIGIN      
        1 ggcaagaagc cccacgccag cgcgagtgtc accttggtga acagactctt gacgaaccag
       61 atcccgacga caatgcgtaa gcctgcgagc gcggcctgcg gatgtcgcat ggttgggacg
      121 ggctcgatgc gggtcgtcat cacgagttct cggtgaagga agtcgagctg tggcgtggaa
      181 cgtgaccgtg cgactggacg ccgggcgaga caacgatatg gcaatgcgac gaaacacgcg
      241 cgtcactctt ctccgttctc gcgcgatcgg gatcgcgatc gtgatcggcg cgacactgtg
      301 cgcgttgact gctggcgcga gcgcgcagaa tggcggcccc ggcgccgcga ctgctccggc
      361 cggcgacggt ccgttccgca ttggcgcgca ggcacgcggc ccgctgcatc gactgtggga
      421 aaacagtctt tccgtcaaac aggagcgcgt ggcgtgcctc ggcggctaca tcgatcatga
      481 cgtagtgtac atcacgcgga tcgaggtgtt ggcgccggca cgcgccgatt cggccgacat
      541 ctccgctgtt gcatcgctgc gcgagtgttc ggcgccgacg tggctcggta cggtgcacac
      601 gcacatcgcg cgattccatg gcctgccata cacgatcttc tcggcgcccg accgatacgt
      661 gatgacgctc tggcgtgagc gctggaaggc accgggcgtc ttctgcatcc tgtactcgga
      721 cgcggatgct aactgcgagt tgggcaacac ggtaagcggc cacgttcagt atgcgcacga
      781 gaagggcaac aatatcgggc tctaacgcga gagcgggctc gcgtcggagg gggcatcacg
      841 ccagacacgc gcgccggtgt tcgcgaacgg tccgacgaac agggcacccg ggctggtcgc
      901 gttgcggaca tcatccgcga gctgccgcgc cgcatcgtcg ccatcgacga tgccgtacac
      961 ggcagggccc cacgagcttt gcccaacgcc agcggcgccc cactccgaca tccggtcgat
     1021 cagcgcagcg ctcggcccgg gggagaatgc ccc
//
LOCUS       DASRVO010000593         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_329405, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000593 DASRVO010000000
VERSION     DASRVO010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329405"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="glmM"
                     /locus_tag="VFF05_07350"
     CDS             complement(<1..582)
                     /gene="glmM"
                     /locus_tag="VFF05_07350"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974558.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZW51658.1"
                     /translation="MQKHYFGTDGIRGRAGEPPITAEFMLRLGRAAGRVLANGSAAPV
                     FIGKDTRVSGYMLESALEAGLVSAGANVRLLGPMPTPAVAFLTRDLHGSAGIVISASH
                     NPYWDNGVKFFDGNGEKLADAAELAIERELDSAGTPSARIGKAARVDDAVQRYAAHCR
                     ATVPGLQLRGLRIVLDCAHGATYHIAPKLFAELG"
     gene            complement(587..949)
                     /locus_tag="VFF05_07355"
     CDS             complement(587..949)
                     /locus_tag="VFF05_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZW51659.1"
                     /translation="MAAPNLAMPRQGILPLTIKDKTALYSAYMPFVRGGGLFVPTAKR
                     YNLGDEVFILLTLMDEKDRLPVPGKIVWITPAGSQGNRAAGIGVQFNDSSDGEAARTK
                     IESILAGILGSDKPTYTM"
     gene            complement(965..>1636)
                     /locus_tag="VFF05_07360"
     CDS             complement(965..>1636)
                     /locus_tag="VFF05_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZW51660.1"
                     /translation="RDDGTPRTEIIVDQIRVLGERLAMTAQFGGMQVARIDPADAMNA
                     SAANALLKTLEEPTPATVIVLIADRPARLPATIRSRCQRIDFHLPPRAQAQGWLIGQG
                     VDAKAADTALDVAAGNPGMALELARSGGLALHAEVDADLRALLDGATSASQVANRWSK
                     SDPERRLWFAAMRIQDSARALTAPADLPKLAEGFDRINRAREQLRGPLRSELVILDAL
                     SALAV"
BASE COUNT          265 a          549 c          543 g          279 t
ORIGIN      
        1 accgagttcg gcgaagagtt tcggcgcgat gtgatacgtg gcgccatgcg cgcaatccag
       61 gacgatgcgc aggccgcgca attgcaggcc cggcaccgtg gcgcggcaat gcgcggcata
      121 acgctgcacg gcgtcgtcca cgcgcgcggc cttgccgatg cgtgcggatg gagtgccggc
      181 ggaatccagc tcacgctcga tcgccagttc cgcggcatcg gcgagcttct cgccattgcc
      241 gtcgaagaat ttgacgccgt tgtcccagta cggattgtgc gaggcgctga tgacgatgcc
      301 agcgctgccg tgcagatcgc gggtgagaaa ggcgaccgcc ggcgtcggca tcggcccgag
      361 caggcgcacg ttggcgccgg cggacacgag tccggcttcg agcgccgatt caagcatgta
      421 gccggatacg cgcgtgtcct tgccgataaa caccggtgcg gcgctgccgt tcgccagcac
      481 gcggcccgcg gcgcggccca ggcgcaacat gaattcggca gtgatcggcg gctcgcccgc
      541 gcgaccgcgg atgccgtcgg tgccgaagta gtgcttttgc atttacctac atcgtgtatg
      601 tcggcttgtc cgacccaagg atacccgcca ggatcgactc gatcttggtc cgcgccgcct
      661 cgccgtcgga cgaatcgttg aactgcacgc cgatacccgc tgcgcgattg ccctgcgatc
      721 cggcgggcgt gatccacacg atctttcccg gcaccggcag gcgatccttt tcgtccatca
      781 acgtgagcag gatgaaaacc tcgtcaccga ggttgtagcg cttggccgtg ggcacgaaca
      841 aaccgccgcc gcgcacgaac ggcatgtagg cgctgtacag cgcggtcttg tccttgatcg
      901 tcagcggcag tatgccttgc cgcggcatgg cgagattggg tgcagccatt gcgcttgctc
      961 ctcgttacac ggccagtgcc gacaacgcgt ccagaatcac cagttccgac cgcaacggcc
     1021 cgcgcaactg ttcgcgtgcg cggttgatgc ggtcgaaccc ctccgctagc ttgggcaaat
     1081 ccgccggcgc ggtcaaggcg cgcgcgctat cctgaatccg catggccgcg aaccacaggc
     1141 gccgttcggg atcggacttg ctccagcggt tggcgacctg cgatgccgag gtcgcgccat
     1201 ccagcaaggc gcgcaggtcg gcgtcgactt ccgcgtgcag ggccagcccg ccggaccggg
     1261 ccaactccag cgccatgccc ggattgcccg cagccacgtc cagcgccgta tctgcggctt
     1321 tcgcatcgac gccctgcccg atcaaccagc cctgcgcctg cgcgcgcggc ggcaggtgga
     1381 aatcgatgcg ctggcaacgg ctgcgaatcg tcgcgggcag acgcgcggga cggtcggcga
     1441 tcaacacgat cacggtggcg ggcgtcggtt cttcgagcgt cttgagcaag gcattcgcgg
     1501 cgctcgcgtt catcgcgtcg gccggatcga tgcgtgcgac ctgcatgccg ccgaactgcg
     1561 cagtcatggc cagacgttcg ccgagcacgc gaatctggtc gacgatgatt tccgtacgtg
     1621 gcgtgccatc gtcgcg
//
LOCUS       DASRVO010000594         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_199642, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000594 DASRVO010000000
VERSION     DASRVO010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199642"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VFF05_07365"
     CDS             <1..1054
                     /locus_tag="VFF05_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008210947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZW51661.1"
                     /translation="LNATGRPVLALDVPSGIDADTGSVLGVAVRADATVTFVAPKRGL
                     FTAEAPDHCGVLVLDRLGLPDALYADASFDARRLDMRRMAHWLPPRARAAHKGDFGHM
                     LAIGGAAGMGGAIRLCAEAALRVGAGLVSAATRQENIAAINAARPEVMAHAATDATAL
                     TPLFARANVIALGPGLGQSDWSRTLWRAALDSHKPAVVDADALNLLAAQTVALPPGSV
                     ITPHPGEASRLLGWSSERIASDRFAAVRELAARHRCVAVLKGAGTLIAHEHGDVAVCP
                     WGNPGMASGGMGDVLTGVIAGLMAQGLNAWRAARLGVALHAQAGDAAARDGQAGTLAS
                     DLFVHLRQQRNGLADD"
     gene            1047..>1215
                     /locus_tag="VFF05_07370"
     CDS             1047..>1215
                     /locus_tag="VFF05_07370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZW51662.1"
                     /translation="MTEIAFNGIDEGELAALARELAPRLRGGGSIHLAGPLGAGKTTF
                     ARALLRALGAGE"
BASE COUNT          173 a          382 c          455 g          205 t
ORIGIN      
        1 tttgaatgcg acgggccggc cggtgctggc gctggatgtg ccatccggca tcgacgccga
       61 caccggatcg gtgctgggcg ttgccgttcg cgccgacgcg acggtaactt tcgttgcgcc
      121 caaacgcgga ttgttcaccg cggaggcacc cgatcattgc ggcgtgttgg tgctcgaccg
      181 cctcggtctg ccggatgcgc tgtatgccga tgcgtctttc gatgcacggc gtctggacat
      241 gcggcgcatg gcgcactggc tgccaccacg cgcgcgcgcg gcgcacaagg gcgacttcgg
      301 gcacatgttg gcgattggcg gcgctgccgg catgggcggt gcgatccgtc tgtgtgcgga
      361 agcggcgctg cgcgtgggcg caggcctggt cagcgcagcg acgcggcagg aaaacatcgc
      421 cgcgatcaat gccgcgcggc ccgaagtgat ggcgcacgcg gcaaccgatg cgactgcgct
      481 gacacctttg ttcgcccgcg ccaatgtcat cgcgctcggg cctgggctgg gccaatccga
      541 ttggagccgg acactttggc gggcggcatt ggactcgcac aaacccgctg tcgtggatgc
      601 ggatgcgctg aacctgcttg ctgcgcaaac ggtcgcgctg ccgcccggca gtgtgatcac
      661 gccgcatccg ggcgaagctt cgcgtttgct gggttggagc agcgaacgca ttgccagcga
      721 ccgctttgcc gcggtgcgcg aactggcggc gcgccatcgc tgcgtggccg ttctcaaggg
      781 cgcgggcacg ctgatagcgc acgaacacgg cgacgtggcg gtgtgcccgt ggggcaatcc
      841 gggcatggcc tcgggcggca tgggcgatgt gctgaccggt gtcatcgcgg gtctgatggc
      901 gcaaggcttg aatgcatggc gcgcggcgag gctaggcgtc gcattgcacg cgcaggctgg
      961 cgatgcggcg gcgcgcgatg ggcaggcggg aacgctcgcg agcgatctgt tcgtgcacct
     1021 gcgtcaacag cgcaacggat tggccgatga ctgaaattgc attcaacggc atcgatgagg
     1081 gcgaactggc cgcgctcgcg cgcgagctcg cgccgcgcct gcgcggcggc ggcagcattc
     1141 atctggccgg tccgctgggc gcgggcaaga ccacgtttgc gcgcgcgctg ttgcgcgcac
     1201 ttggcgccgg cgaac
//
LOCUS       DASRVO010000595         1200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_251329, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000595 DASRVO010000000
VERSION     DASRVO010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251329"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1116
                     /gene="hemH"
                     /locus_tag="VFF05_07375"
     CDS             106..1116
                     /gene="hemH"
                     /locus_tag="VFF05_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZW51663.1"
                     /translation="MPEPNRESTPEVAVLLVNLGTPSAPTSSAVRRYLGQFLSDRRVI
                     DYPRWLWLPLLYGVILRVRPRRSARAYAKIWMPQGSPLLANSQALANKLAAELVGAKI
                     QVALGMTYGEPAIGDAIHNLLADGVRRILLLPLFPQYSATSTAAGYDAVFAALRAVRR
                     VPEVRSIADYHAEPGYIDALAASVEKYWFTHGRGERLLLSFHGIPERYVNAGDPYRDQ
                     CLETARRLRERLGISETELVVAFQSRVGRERWLAPYTDETLAQLAHSGVRHVQALCPG
                     FAVDCLETLEEIAIRGREQFLAAGGQRLDYMPALNATDAHVRVLANLIRRHTQGWTDA
                     GA"
BASE COUNT          201 a          398 c          388 g          213 t
ORIGIN      
        1 cacttgcgtc gccagccacc gaatgaccgc aatccctgcc gctccggtcg atcaagcagc
       61 acaagatatt gggttattcg cgcctcccgg aattcatcca cccgtatgcc agagccaaat
      121 cgcgagtcca cgcccgaggt agccgttctg ctcgtcaatc tgggcacgcc gtcggcaccc
      181 acatcgtcgg ccgtacggcg ttacctgggg caattcctga gcgaccggcg cgtcatcgac
      241 tatccgcgct ggctgtggct gccactgttg tacggcgtga tcctgcgcgt acgcccgcgg
      301 cgctcggcgc gcgcctacgc gaaaatctgg atgccacagg gatcgccgct gctggcgaac
      361 agccaggcgc tggcaaacaa gctggccgcg gagttggtcg gagccaaaat ccaggtcgcg
      421 ctcggcatga cctatggcga gccggcgatt ggcgatgcaa tccacaatct gcttgccgat
      481 ggcgtgcggc gcatcctgtt gctgccgctg ttcccgcaat attcggcgac gtccaccgcg
      541 gcggggtacg atgccgtctt cgccgcgctg cgtgccgtac ggcgggtgcc ggaagtgcgc
      601 agcattgccg actatcacgc cgaacccggc tacatcgacg cgctcgccgc gagcgtggaa
      661 aagtattggt tcacgcacgg tcgcggcgaa cgcctgttgc tttcgtttca cggaattccg
      721 gaacgctacg tcaacgccgg cgatccgtac cgcgatcaat gtctggagac ggcgcggcgg
      781 ctgcgtgagc gcctgggcat atccgaaacg gaactggtgg tcgcgttcca gtcgcgcgtc
      841 ggccgcgagc gctggctggc gccgtacacc gacgagacgc tcgcgcaact cgcgcattcc
      901 ggcgtgcgcc acgtgcaggc gttgtgtccg ggattcgcgg tagattgcct ggagaccctg
      961 gaagaaatcg cgatacgcgg acgcgagcaa ttccttgccg cgggcggcca acgcctggat
     1021 tacatgcctg cgctgaatgc gacggatgcg catgtgcgcg tgctcgcgaa cctgatccgg
     1081 cgccacacgc aaggctggac cgatgccggt gcgtgacatc gaattggcct tgcccggcct
     1141 gcgcatcgcc gcgcgtgcct ggggagacac atcccggccg aagttgctcg ccttgcacgg
//
LOCUS       DASRVO010000596         1208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_172365, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000596 DASRVO010000000
VERSION     DASRVO010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172365"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VFF05_07380"
     CDS             complement(<1..1141)
                     /locus_tag="VFF05_07380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW51664.1"
                     /translation="MSDGNPLACEIDQKIEAAGYELTLLDRVDELKEVLAAFAPHLII
                     VDAPFQDALESVGDMVKAARTRLGRRLALLAFSDSGELPVRLRAMRAGADSFIPLPAQ
                     SGDVMGRIAELLDTETSDPFRVLIVEDDRSQAMFAESILRKAGMHTLAATDPLAALDE
                     LDAFKPELILMDLYMPNCSGMELTAIIREREAFINTPIVFLSGEHDEEKHFEALNAGG
                     DDFLSKPIRPKHLISAVTNRVRRSRTLGRRAQASNPRDPVTGLYQRAHVLDRLNALLA
                     GDEADGGLIYIELDGAGQIRERVGLTSFDALFNQIGAFLAAHIGAHDLAARYGDTSFV
                     LLCQGDADALARLATDLRERTTREVFEHEGKSLAVSLSFGICGFGA"
BASE COUNT          203 a          375 c          421 g          209 t
ORIGIN      
        1 ccgcgccgaa gccgcagatg ccgaaactca aggacaccgc gagcgacttg ccttcgtgtt
       61 cgaacacctc gcgcgtggtg cgctcgcgca ggtccgtcgc caggcgcgcg agggcatccg
      121 catcgccctg gcacagcagc acgaagctgg tgtcgccgta gcgcgcggcc agatcgtgcg
      181 cgccgatgtg cgcggcgagg aaggcgccga tctggttgaa cagggcgtcg aagctggtca
      241 gaccgacgcg ctcgcggatc tgtccggcgc cgtcgagctc gatgtagatc agtccgccgt
      301 cggcctcgtc gccggcgagc agggcattga gccggtcgag gacgtgggcg cgctggtaca
      361 ggccggtcac cgggtcgcgc ggattgctcg cctgcgcgcg gcggccgagc gtgcgcgagc
      421 ggcgcacgcg gttcgtcacc gcggaaatca ggtgcttggg acggatcggc ttggacagga
      481 aatcgtcgcc gccggcattg agtgcttcga agtgtttttc ctcgtcgtgc tcgccggaaa
      541 ggaacacgat cggcgtgttg atgaacgctt cgcgctcgcg gatgatcgcg gtgagttcca
      601 tgcccgaaca gttgggcatg tacagatcca tgagaatcaa ttcgggtttg aatgcgtcga
      661 gctcgtcgag cgcagcgagc ggatcggtcg ccgccagcgt gtgcatgccg gccttgcgca
      721 ggatcgactc ggcgaacatc gcctgcgaac ggtcgtcctc gacgatcagc acgcggaagg
      781 gatcggatgt ttccgtatcc agcaattcgg cgatgcggcc catcacgtcg ccggactgcg
      841 ccggcagcgg aatgaagctg tccgcgccag cacgcattgc gcgcaggcgc acgggcaatt
      901 cgccggaatc ggaaaacgcg agcagggcca agcgccggcc cagccgcgtg cgcgcggcct
      961 tgaccatgtc accgacggat tcgagcgcgt cctggaatgg cgcatcgacg atgatgaggt
     1021 gcggtgcgaa cgcggcgagc acttccttga gttcgtcgac acgatcgagc agggtcaatt
     1081 cgtatccggc cgcctcgatt ttctggtcga tctcgcaggc cagcggatta ccgtcgctca
     1141 aatggaatac cttgcgttgc tcggccgtgg tcggcgctgc cgcaatcggt gctgctgcaa
     1201 tcgctgcc
//
LOCUS       DASRVO010000597         1169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_278066, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000597 DASRVO010000000
VERSION     DASRVO010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278066"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..87
                     /gene="clpS"
                     /locus_tag="VFF05_07385"
     CDS             <1..87
                     /gene="clpS"
                     /locus_tag="VFF05_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416477.1"
                     /note="involved in the modulation of the specificity of
                     the ClpAP-mediated ATP-dependent protein degradation;
                     binds to the N-terminal domain of the chaperone ClpA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HZW51665.1"
                     /translation="EVAETKVTQVNEFSRSHQHPLLCTLEKA"
     gene            127..>1169
                     /locus_tag="VFF05_07390"
     CDS             127..>1169
                     /locus_tag="VFF05_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014037145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZW51666.1"
                     /translation="MFSKDLELSIGQCYKDAREQRHEFMTVEHLLLALLENPSASAVL
                     RACGADSAKLGKDLKNIIVETVPVLPANDERDTQPTLGFQRVLQRAVYHVQSSGRKEV
                     TGANVLVAIFGEKDSHAVYFLNQQEVSRLDVVNYISHGIAKIGQENQPQSGAETGKEG
                     DAGEEPRGNPLTDFAANLNELAKQGKIDPLIGRAEEIERTIQVLCRRRKNNPLFVGEA
                     GVGKTALAEGLAKRIVEGDVPEVLKTCTIYALDMGALVAGTKYRGDFEKRLKAVIAQL
                     KKEPHAILFIDEIHTIIGAGSASGGTMDASNLIKPVLASGELRCIGSTTFQEFRGVFE
                     KDRALARRFQKIDV"
BASE COUNT          250 a          365 c          364 g          190 t
ORIGIN      
        1 gaagtcgccg aaaccaaggt cacccaggtc aacgagttct cgcgttcgca ccagcatccc
       61 ctgttgtgca cgttggaaaa ggcgtaaaag accccatcta ggcacgtgtc cctctccgag
      121 tacaccatgt tcagcaaaga cctcgaactc tccattggcc agtgctacaa ggacgcgcgc
      181 gagcagcgtc atgaattcat gaccgtggag cacttgctgc tggcgctgct ggaaaatcca
      241 tcggcgtcgg cggttctgcg cgcctgcggc gcggacagcg ccaagctcgg caaggatttg
      301 aagaacatca tcgtcgagac ggtgccggta ttgccggcca acgacgagcg cgacacacaa
      361 ccgacgctgg gcttccagcg cgtgctgcaa cgcgccgtct atcacgtgca atcgtccggg
      421 cgcaaggaag tcaccggcgc caatgtgctg gtggcgatct tcggggagaa ggattcgcac
      481 gccgtgtact tcctcaacca gcaggaagtc tcgcgcctgg acgtggtcaa ttacatctcg
      541 catggcattg ccaagatcgg ccaggaaaac cagccgcaat cgggcgccga aaccggcaag
      601 gaaggcgatg ccggcgaaga gccgcgcggc aacccgctca ccgatttcgc cgccaatctc
      661 aatgagctcg ccaagcaagg caagatcgat ccgctgatcg gccgtgccga ggaaatcgag
      721 cgcacgatcc aggtgttgtg ccggcgacgc aagaacaatc cgctgttcgt tggcgaggct
      781 ggcgtgggca aaacggcgct tgcggaaggc ctggccaagc gcatcgtcga aggcgacgta
      841 cccgaggtgc tcaagacctg cactatctac gcgctggaca tgggcgcgct ggttgccggc
      901 accaagtacc gcggcgattt cgaaaagcgc ctgaaggcgg tcatcgcgca gctcaagaag
      961 gagccgcacg cgatcctgtt catcgacgag atccacacga tcatcggcgc cggctcggcc
     1021 agcggcggca cgatggatgc ctcgaacctg atcaagccgg tgctcgcctc gggcgagctg
     1081 cgctgcatcg gctcgaccac gttccaggaa ttccgcggcg tgttcgagaa ggatcgcgcg
     1141 ctggcgcggc gcttccagaa gatcgacgt
//
LOCUS       DASRVO010000598         4224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_121823, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000598 DASRVO010000000
VERSION     DASRVO010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121823"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /gene="glmU"
                     /locus_tag="VFF05_07395"
     CDS             complement(<1..985)
                     /gene="glmU"
                     /locus_tag="VFF05_07395"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZW51667.1"
                     /translation="MNPLHIVILAAGEGKRMHSALPKVLLPLAGKSMLAHVIAAAREL
                     NPARIHVVFGHRGDAVRAAFADQSDLDWVHQAEQRGTGHAMQLALAQVAEAARVLVLY
                     GDVPLIRAATLRPLCDADAPLALLAAELFDPRGYGRVIRDGLGQVRAVIEDRDCAPDQ
                     RSITTVNTGILAADARRLRVWTHNLDNRNAQGEFYLTDVFAQASAEGMPAAILNCRDE
                     REAFGANDAWQLAQLERHFQQRQAKALCEQGVRLADPARFDLRGGLVCGRDVEIDVDA
                     IFEGRVELGDGVRIGPFCRIKDSTLAAGTVVRAHCDLDGAVAQGACVIGPFA"
     gene            complement(1100..1522)
                     /locus_tag="VFF05_07400"
     CDS             complement(1100..1522)
                     /locus_tag="VFF05_07400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011407577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HZW51668.1"
                     /translation="MTTIRCDIVSAEAEIFHGQAKMVIATGEAGELGIAPRHAPLITR
                     LKPGQVRVLAENGEEQFFYVSGGILEVQPQVVTVLADTAIRAKDLDEAAARHAKENAE
                     RLLANRADAVEIAQAQAELMQAIAQLRAIEQLRKQMRH"
     gene            complement(1532..2965)
                     /gene="atpD"
                     /locus_tag="VFF05_07405"
     CDS             complement(1532..2965)
                     /gene="atpD"
                     /locus_tag="VFF05_07405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZW51669.1"
                     /translation="MSQGKVVQIIGAVVDVEFPRDAVPKVYDALKVDGTAVTLEVQQQ
                     LGDGVVRTIALGSTDGLKRNLVATNTNAGIKVPVGTATLGRIMDVLGTPIDEVGPIGA
                     KDHWEIHRPAPSYDEQAAANDLLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMLE
                     LINNIATQHSGLSVFAGVGERTREGNDFYHEMIEAGVVNLKDLPQSKVAMVYGQMNEP
                     PGNRLRVALTGLTMAEYFRDEKDASGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSA
                     VGYQPTLADEMGRLQERITSTKTGSITSIQAVYVPADDYTDPSPATTFAHLDSTVALS
                     RDIASLGIYPAVDPLASTSRQLDPNVIGVEHYETARRVQGTLQRYKELKDIIAILGMD
                     ELSEEDKATVGRARKIERFFSQPFHVAEVFTGAPGKYVSLKDTIRGFKMICDGECDHL
                     PEQAFYMVGGIEEAFEKAKKMTEKKAA"
     gene            complement(3007..3870)
                     /gene="atpG"
                     /locus_tag="VFF05_07410"
     CDS             complement(3007..3870)
                     /gene="atpG"
                     /locus_tag="VFF05_07410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010371995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZW51670.1"
                     /translation="MAGAREIRTKIKSVANTRKVTRALEMVSASKIRKAQDLMRGSRP
                     HARAMRQVVGHIAKANTEFKHPFLSTRDTVKRVGYIVVSTDRGLCGGLNSNLFRKLLA
                     EIRDWNEKNVEVDIVAIGTKANVFFRRLKVNLVGSATHMGEKPKIEQLIGVIKIMLDN
                     YSAGRIDRLTLAYNDFVNTMTQKPTLDQLLPLPPSESLATAHDWDYLYEPDAESVLEH
                     VLTRYIESLVYQATLENLASEHAARMVAMKSAGDNAKKAIDSLTLVYNKVRQAAITQE
                     ISEIVSGAAAV"
     gene            complement(3877..>4224)
                     /locus_tag="VFF05_07415"
     CDS             complement(3877..>4224)
                     /locus_tag="VFF05_07415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161761.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZW51671.1"
                     /translation="QYRELAAFAQFASDLDAATRAQLERGQRVTELMKQKQYAPLSIA
                     QLALSLYAAEKGYLDDLPIAKILPFEAALHAHFAANYADWVKAANDKADWSDAIEATL
                     KKGIEEFKKTGSW"
BASE COUNT          653 a         1333 c         1409 g          829 t
ORIGIN      
        1 gtgcgaacgg gccgatgaca caggcgcctt gagcgaccgc gccgtccagg tcgcaatgcg
       61 cgcgcacgac ggtaccggcg gcgagggtgg aatccttgat ccggcagaac ggaccgatgc
      121 gtacgccatc gccgagttcg acgcggcctt cgaatatcgc atcgacgtcg atttccacat
      181 cgcgcccgca gacaagaccg ccgcgcagat cgaaacgtgc cggatcggcc aggcgcacgc
      241 cctgttcgca cagcgccttc gcctggcgct gctggaaatg acgctccagt tgcgcgagct
      301 gccaggcatc gttcgcgccg aacgcttcgc gctcgtcgcg gcaattcagg atcgccgcag
      361 gcatgccttc agcggacgcc tgggcgaaca catcggtaag gtagaactcg ccctgcgcgt
      421 tgcggttgtc gaggttgtgc gtccacacgc gcaggcggcg cgcgtcggcg gcaaggatgc
      481 cggtattcac ggtggtgatg ctgcgctggt cgggcgcgca gtcgcgatcc tcgatcacgg
      541 cgcgtacctg gcccaaccca tcgcggatca cgcggccgta accgcgcgga tcgaatagtt
      601 cggcggcgag cagggcgagc ggcgcatccg cgtcgcacag cggcctgagc gtggcggcgc
      661 gaatcagcgg cacgtcgccg tacagcacca gcacgcgtgc cgcttccgcc acttgggcca
      721 gcgcaagctg catggcgtgg ccggtgccgc gctgttcggc ttgatgcacc caatccagat
      781 cggattgatc ggcaaacgcg gcacgcaccg cgtcgccgcg atggccgaac acgacatgga
      841 tccgcgccgg attcagttcg cgcgcagccg cgatcacatg cgccagcatc gattttccgg
      901 ccaacggcag cagcactttc ggcagtgcgg aatgcatgcg tttgccttca ccggcggcga
      961 ggatgacgat gtggagggga ttcatgcgcc tacgatagcg cacgaatgcg gcgcggatcg
     1021 agttttttat ggacggagcg aaggcgcaaa caaaaacgcc ggcttgcgcc ggcgttttgc
     1081 ttcaactggt ggggcgcact caatgccgca tctgcttgcg caactgctcg atcgcgcgca
     1141 actgcgcgat ggcctgcatg agttcagcct gggcctgcgc gatttccacc gcgtcggcgc
     1201 ggttcgcgag caggcgttcg gcgttttcct tggcgtggcg tgcggcggcc tcgtccagat
     1261 ccttggcgcg gatcgcggta tcggcgagca cggtcacgac ctgcggctgc acttcgagga
     1321 tgccgccgga aacgtagaag aactgttcct cgccgttttc ggcgagcacg cgcacctggc
     1381 ccggcttcag gcgcgtgatc agcggcgcgt gacgcggcgc gatgccgagt tcgccggcct
     1441 cgccggtggc gatgaccatc ttcgcctggc cgtggaagat ctcggcctcg gcgctgacga
     1501 tgtcgcagcg gatggtggtc atgtccgtgc cttaggccgc tttcttctcg gtcatcttct
     1561 tggccttctc gaaggcttcc tcgatgccgc cgaccatgta gaacgcctgt tccggcaggt
     1621 gatcgcattc gccgtcgcag atcatcttga agccgcggat ggtgtccttg agcgagacgt
     1681 acttgcccgg cgcgccggta aacacttcgg cgacgtggaa gggctgcgag aagaagcgct
     1741 cgatcttgcg cgcgcggccg acggtggcct tgtcttcttc gctcaattcg tccatgccga
     1801 ggatcgcgat gatgtccttc aattccttgt agcgctgcag ggtgccctgc acgcggcgcg
     1861 cggtctcgta gtgctccacg ccgatcacgt tcggatcgag ctggcggctg gtcgaggcga
     1921 gcggatccac ggccgggtag atgccgagcg aggcgatgtc gcgcgacagc gcaacggtcg
     1981 aatccagatg cgcgaaggtc gttgccggcg acggatcggt gtaatcgtcc gccggcacgt
     2041 acacggcctg gatcgaggtg atcgaaccgg tcttggtcga ggtgatgcgc tcctgcaggc
     2101 ggcccatttc gtcggccagg gtcggctggt aacccacggc ggacggcatg cggccgagca
     2161 gcgcggacac ttcggtgccg gccagcgtgt agcggtagat gttgtcgacg aagaacagca
     2221 cgtcgcggcc cttgccggaa gcgtccttct cgtcgcggaa gtactcggcc atggtcaggc
     2281 cggtgagcgc gacgcgcagg cggttgccgg gcggctcgtt catctggccg tagaccatcg
     2341 ccactttcga ctgcggcaga tccttcaggt tgaccacgcc cgcttcgatc atctcgtgat
     2401 agaagtcgtt gccctcgcgg gtgcgctcgc cgacgccggc aaacacggac aggcccgagt
     2461 gctgcgtggc gatgttgttg atcagttcga gcatgttcac ggtcttgccc acgcccgcgc
     2521 cgccgaacag gccgaccttg ccgcccttcg cgaacggcgc gaccaggtcg atgaccttga
     2581 tgccggtttc gagcagatcg ttcgccgcgg cctgctcgtc gtagctcggc gcggggcggt
     2641 ggatttccca gtggtccttc gcgccaatcg ggccgacttc gtcgatcggc gtgccgagca
     2701 cgtccatgat gcggccgagg gtggcagtgc ccaccggcac cttgatgccg gcgttggtgt
     2761 tcgtggcgac gaggttgcgc ttgaggccgt cggtggagcc gagcgcgatc gtgcgcacga
     2821 cgccatcgcc gagctgctgc tgcacttcga gcgtgaccgc agtgccgtcg accttcagcg
     2881 cgtcgtacac cttcggtacc gcatcgcgcg ggaattcgac gtcgaccacc gcgccgatga
     2941 tttggactac tttgccttga ctcatcttcg tgtcctcgat tctcaatctt ttggttggct
     3001 ctgcgcttaa accgcagcag ctccactcac aatttcggaa atttcctggg tgatcgccgc
     3061 ctgacgaacc ttgttgtaga ccagggtcag cgaatcgatc gccttcttcg cgttgtcgcc
     3121 cgcggatttc atcgcgacca tgcgtgcggc gtgttcgctc gccagatttt ccagcgtcgc
     3181 ctggtacacc agcgactcga tgtagcgggt cagcacatgt tcgagcacgg attcggcatc
     3241 cggctcgtac aggtaatccc agtcgtgcgc ggtggcgagc gattccgacg gcggcagcgg
     3301 caggagctga tccagtgtcg gcttctgcgt catcgtgttg acgaaatcgt tgtaggccag
     3361 ggtcaggcgg tcgatgcggc cggcactgta gttgtcgagc atgatcttga tcacgccgat
     3421 gagctgctcg atcttcggct tctcgcccat gtgtgtcgcg ctgccgacca ggttcacctt
     3481 gagtcggcgg aagaacacgt tcgccttggt gccgatggcg acgatgtcga cttcgacgtt
     3541 cttctcgttc cagtcgcgga tttccgcgag cagcttgcgg aacaggttgg aattcaagcc
     3601 tccgcacagg ccgcggtcgg tggagacgac gatgtagccg acgcgcttga cggtgtcgcg
     3661 cgtggacagg aacggatgct tgaactcggt gttggccttg gcgatgtggc cgaccacctg
     3721 gcgcatcgcg cgcgcgtgcg ggcgcgagcc gcgcatcagg tcctgcgcct tgcggatctt
     3781 ggacgccgag accatctcca gcgcgcgcgt caccttgcgc gtgttcgcga cggacttgat
     3841 tttggttcgg atttcacgtg caccggccat ggcggcttac cagctacccg tcttcttgaa
     3901 ttcctcgatg cccttcttga gcgtcgcttc gatggcgtcg ctccagtcgg ccttgtcgtt
     3961 cgcggccttc acccagtcgg cgtagttggc agcgaagtgg gcgtgcaggg ccgcctcgaa
     4021 cggcaggatc ttggcgatcg gcagatcgtc gaggtaaccc ttctcggccg cgtacagcga
     4081 cagcgcgagt tgcgcgatgg acagcggcgc gtactgcttc tgcttcatca actcggtcac
     4141 gcgctggccg cgctccaact gcgcgcgcgt ggcggcgtcg aggtcggagg cgaactgcgc
     4201 gaacgccgcg agctcgcggt actg
//
LOCUS       DASRVO010000599         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_146637, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000599 DASRVO010000000
VERSION     DASRVO010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146637"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFF05_07420"
     CDS             <1..353
                     /locus_tag="VFF05_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HZW51672.1"
                     /translation="ALDASRGMGKDESRAVEPGITVRTIGFEKLVQELARDHGLFLMD
                     RKGTPIRGSAITGNPCFILTDHMPMPKKSLSTLERLGAEEISLGSKMLFASQCVLLIH
                     NELDHRNQSCQGGF"
     gene            377..>1156
                     /locus_tag="VFF05_07425"
     CDS             377..>1156
                     /locus_tag="VFF05_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51673.1"
                     /translation="MHWRAIVGVVVLAVWLGVPSTGEAAPPVASAAAPNLEQRSKSCI
                     DTILADARTALPAAEQGLAEARTAGDRNAESRFLRCVGYAHESMGENRAAFAAYAEAV
                     TAADAGGDREALADALALRGEQLHANGDFDGAIADFKRAYDLDHAAGLETGETYVLNA
                     MANLYADPNVGDFDKALGYYRDLLERHRRDGNRGEMATAYFNIGATLESRHEYTKALE
                     DYRRALDLYRTLDNAESTAETRRVIGAALVKLGRAKAAIGWI"
BASE COUNT          226 a          341 c          392 g          197 t
ORIGIN      
        1 aggcgctgga tgcatccaga ggcatgggca aggacgaaag ccgcgcggtc gagccgggca
       61 tcaccgtgcg cacgatcggt ttcgagaagc tcgtgcagga attggcgcga gaccatggcc
      121 tgttcctgat ggacaggaag ggcacgccga ttcgcggcag cgcaattacc ggcaatcctt
      181 gtttcatact caccgatcac atgccgatgc cgaagaagag tttgagcacc ctggagcgtc
      241 tgggcgccga ggaaatcagc ctcggctcga aaatgctgtt cgcgtcgcag tgcgtactgt
      301 tgatccacaa cgaactggac catcggaacc aatcgtgtca gggagggttc tgactgcgaa
      361 aaatcccggc ccacgattgc attggcgtgc aatcgtgggc gtggtcgtgc tcgctgtctg
      421 gctcggggtg ccgtcgacgg gggaagctgc accaccggtg gcgtcagcgg cggcgccgaa
      481 cctggagcaa cgttcgaaat cctgcatcga caccatcctc gcggatgcca ggacggcgct
      541 tccggccgcc gagcaggggc tggccgaggc gcgcacggcc ggcgaccgca atgccgagtc
      601 gcgcttcctg cggtgcgtcg gttacgcaca cgaatcgatg ggagaaaacc gcgcagcctt
      661 cgccgcctac gccgaagccg tgacggccgc cgatgcgggc ggcgatcgcg aggcgttggc
      721 ggatgccctg gcattgcgcg gtgagcaact gcatgccaat ggcgatttcg acggcgccat
      781 cgccgatttc aagcgcgcct acgatctgga ccacgcagcc gggctggaaa cgggcgaaac
      841 ttacgtgctc aatgccatgg ccaacctgta cgccgatccc aatgtcggcg atttcgacaa
      901 ggcgctgggt tattaccgcg atttgctcga gcggcatcgc cgtgacggca atcgcgggga
      961 aatggcgacg gcgtatttca atatcggcgc gacgctggag agccggcacg aatacaccaa
     1021 ggcgctggag gattaccgcc gtgcgctgga tctgtatcgc actctggaca atgccgaatc
     1081 gacggcggaa acgcgtcgtg tgatcggtgc ggccctggtc aagttgggtc gggcaaaggc
     1141 ggcgatcggc tggatc
//
LOCUS       DASRVO010000600         1214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_44497, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000600 DASRVO010000000
VERSION     DASRVO010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44497"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VFF05_07430"
     CDS             <1..1038
                     /locus_tag="VFF05_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HZW51674.1"
                     /translation="AIIGIAATIFVWALPFAEDQFDRIKNPYLRHAIGMALVGMLFYA
                     LFYLWGHYFVEGVGYATVQALLVGQMTPMLYILPLLFLGKLVAISLTIGSGGSGGIFS
                     PSLYMGAMLGGAFGALVDAIHPVAGATPETFAIVGMAAMVGGGTGAAMTAVTMIFEMT
                     RDYGMVMPMILAVALSVGVRRMLSRQSVYTIKLVARGHFIPEALHANMFLVRHANHVM
                     EKDVLVLPANTDFNSFLRQPEHNGALKHVVVTENERVVGVMRVNTGFRRGLEAAYTGV
                     TLGDVAQKNFTVEHEDDIAFDVIGRMWREGAAMAVVVRKTATALAEDVRGIISKEHVA
                     DSVAQSIKPYA"
BASE COUNT          211 a          384 c          388 g          231 t
ORIGIN      
        1 gcgatcatcg gcattgcggc aacgatcttt gtgtgggcac tgcccttcgc cgaggatcag
       61 ttcgaccgga tcaagaaccc gtatttgcgt cacgccatcg gcatggccct ggtcggcatg
      121 ttgttctacg cgctcttcta cctgtgggga cattacttcg tcgagggcgt cggttacgcc
      181 accgtccagg cgctgctggt cgggcagatg acgccgatgc tctacattct tccgttgctt
      241 ttcctcggca agctcgtggc gatctcgctc accatcggtt cgggcggctc cggggggatt
      301 ttctcgccat cgctgtacat gggcgcgatg ttgggcggcg cgttcggcgc gctcgtcgac
      361 gcgatccatc cggtagccgg cgccacgccc gagaccttcg ccatcgtcgg catggcggcg
      421 atggtcggcg gcggcaccgg cgcggcgatg accgcggtga cgatgatctt cgagatgacg
      481 cgcgattacg gcatggtcat gccgatgatc ctcgcggtgg cgctcagcgt cggcgtgcgc
      541 cggatgctgt cgcgccagag cgtctacacg atcaagctgg tggcgcgcgg ccatttcatc
      601 ccggaggcgc tgcacgccaa catgttcctc gtgcgccacg cgaaccacgt catggagaag
      661 gatgtcctgg tgctgccggc gaacaccgat ttcaacagct ttctgcgcca gcccgagcac
      721 aacggcgcgc tgaaacacgt cgtggtcacc gaaaatgagc gggttgtcgg cgtgatgcgg
      781 gtaaacaccg gctttcgccg cggcctcgaa gccgcctata ccggcgtcac gctcggcgac
      841 gtggcgcaga agaatttcac cgtcgagcac gaggacgaca tcgccttcga tgtcatcggc
      901 cgcatgtggc gcgagggcgc cgcgatggcg gtggtcgtgc ggaaaacggc gacggcgctc
      961 gccgaagacg tacgcggcat catctcaaag gaacacgtcg ccgattcggt cgcccagagc
     1021 atcaagccct atgcgtgacg aagcgcgtcg gacgatctga acggctgcgc cgtcgcagaa
     1081 tttaaacgct gagcgaccgg cgtccgccgg cttctacggt tgatgtcgag catcaggccg
     1141 caacccgcag tgcgaagcac tttttgctga attcaccaat cgaacccttt cccaccaacc
     1201 agccagaggc gaga
//
LOCUS       DASRVO010000601         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_354682, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000601 DASRVO010000000
VERSION     DASRVO010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354682"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFF05_07435"
     CDS             <1..387
                     /locus_tag="VFF05_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZW51675.1"
                     /translation="IDSMRDDDGEWRIRQYTGSTNTVRALPRRTLDWVDEVQGRGAGE
                     IVLNCMGSDGVRDGYDLEQLAAIRARCRVPLVASGGAGSKEHFRDAFTEVDADGALAA
                     SVFHSGAIAIPPLKAWLRSQGVHVRP"
     gene            384..1001
                     /gene="hisIE"
                     /locus_tag="VFF05_07440"
     CDS             384..1001
                     /gene="hisIE"
                     /locus_tag="VFF05_07440"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HZW51676.1"
                     /translation="MNELDLDWSKGNGLLPAIVQDARSGAVLMLGYMNAEALARTQQG
                     GKVTFYSRSKQRLWTKGETTGNHLLLKSVRADCDSDALLIQAEPIGPTCHQGTRSCFG
                     DGSEPGLAFLAVLDTLVARRARELPVGSYTTALFERGIRRIAQKVGEEGVETALAATT
                     GDDAELLDEAADLLFHLIVLLCARGKSLSDVVDRLRTRHDVAAIA"
     gene            1111..1467
                     /locus_tag="VFF05_07445"
     CDS             1111..1467
                     /locus_tag="VFF05_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZW51677.1"
                     /translation="MDETESHLWKFQKELSSGIVSLVLLGVLSRARQPMYGYQIVKRL
                     DETGAGVLSGKQSALYPVLRNLAAAGLLEGEVEPSVTGPPRRYYRITPLGRRVLREWS
                     LAWIDTRDFVDSTLVE"
     gene            1536..2447
                     /locus_tag="VFF05_07450"
     CDS             1536..2447
                     /locus_tag="VFF05_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HZW51678.1"
                     /translation="MSTVAPRSVAEYLEQLRAALAGADPALVQDALYDAEEHLRGELR
                     ESRDKPEAEILARIVGSYGAPEEVAEIYVDKENVVRQALHAPPQRPRASVLGRFFAVG
                     SDPRAYSALFYMLLSLATGIFYFTWTVTGLSLSLGMSILIFGIVVAVLFFGTTRVLSL
                     VEGRIVEVMLGERMPRRPLYPPREGSMFKRIGGMFKDPRTWSTLLYFVLMLPLGIVYF
                     TFAVTGIALSLSFILAPLAHLGWMLGLVQVDWADHIHFNEQLDVPWQLADPLLFVLGI
                     VLLFGLLHLARGIGKFQGGLAKALLVN"
     gene            2462..>2738
                     /locus_tag="VFF05_07455"
     CDS             2462..>2738
                     /locus_tag="VFF05_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HZW51679.1"
                     /translation="MTGDFRKTLDGKPPRIIAHRGASGHRPEHTLEAFALALAQGADI
                     LEPDLVASRDGVLFARHDLGLSRSTDVAARPQFAERAREIGGERDWWI"
BASE COUNT          457 a          837 c          908 g          536 t
ORIGIN      
        1 atcgattcca tgcgcgatga cgacggcgaa tggcgcatcc gccaatacac cggttccaca
       61 aacacggtgc gcgccttgcc gcggcggacg ctggattggg tcgacgaagt gcaaggccga
      121 ggcgccgggg agatcgtgct caactgcatg ggttccgatg gcgtgcgcga cggctacgat
      181 ctggaacaac tcgccgcgat acgcgcgcgt tgccgcgtac cattggtcgc gtctggcggt
      241 gccgggagca aagagcactt tcgcgatgcg ttcaccgaag tcgatgccga cggcgcgctc
      301 gcggccagcg tattccactc cggtgccatc gcgatcccgc cactgaaggc atggcttcgc
      361 agccagggag tgcatgtacg gccatgaacg agcttgacct ggattggagc aagggcaacg
      421 gcctgctgcc cgccatcgtg caggacgcgc gcagcggcgc cgtgctcatg ttgggctaca
      481 tgaacgccga ggcgcttgcg cggacgcagc aaggcgggaa agtgactttc tacagccgca
      541 gcaaacaacg cctgtggacg aaaggcgaga cgacgggcaa tcatttgctg ctgaaatcgg
      601 tacgtgccga ctgcgatagc gacgcgctgt tgattcaggc cgaaccgatc ggcccgacct
      661 gccaccaggg cacgcgcagc tgtttcggcg acggttcgga gcctgggctc gcgttcttgg
      721 ccgtgctcga cacgctggtc gcgcggcgtg cgcgcgagtt gcccgtgggc agctacacga
      781 ccgcgctctt cgagagaggc atacggcgca tcgcgcagaa agtcggcgag gagggcgtgg
      841 aaaccgctct tgctgccacg acgggcgatg acgcggaatt gctcgatgag gctgccgatc
      901 tattgtttca cctgatcgtg ctgctgtgcg cacgcggcaa gtccttgtcc gatgtcgtcg
      961 accgtttgcg gacacgtcac gacgtcgctg caatcgccta gcccaggttt cggtccaaag
     1021 ccggcgggac gccggcatga cttccggccc attcactatt gtcaagtcaa tactatagtt
     1081 gacttggcgt catggcgagg tcgatgcaac gtggacgaaa ccgaaagtca tctgtggaag
     1141 ttccagaagg aactgagctc cggcatcgtt tcgctggtct tactcggcgt gctttcgcgc
     1201 gcgcgccagc ccatgtacgg ctaccagatc gtcaagcgcc tggacgaaac cggcgccggc
     1261 gtgcttagcg gcaagcaaag cgcgctgtat ccggtgctgc gcaacctggc cgcggcaggc
     1321 ttgctcgaag gtgaagtcga accttcggtg accgggccgc cgcggcgcta ctaccgcatc
     1381 acgccgctcg gccggcgcgt gttgcgcgaa tggagcctgg cctggattga cacccgtgat
     1441 ttcgtggata gcactcttgt tgaatagtgc gggcgtgatg cccgctttgt gatcttcgcc
     1501 ggcatggatg acggcaaata catcggagat tgagtatgag caccgtagcg ccgcgttcag
     1561 ttgcggaata cctcgaacag ttgcgcgccg cgctcgccgg cgccgatccc gcgctggtcc
     1621 aggacgcctt gtacgatgcc gaggagcatc tgcgcggcga actgcgcgag agtcgggaca
     1681 aacccgaggc cgagatattg gcgcggattg tcggcagcta cggcgcaccg gaggaagtcg
     1741 ccgagatcta tgtcgacaag gaaaatgtcg tgcgtcaggc tctgcatgct ccgccgcagc
     1801 gaccgcgcgc atccgtgctt ggccgcttct ttgcggtggg ttccgatccg cgcgcgtatt
     1861 cggcgctgtt ctacatgttg ttgtcgctgg cgacggggat tttctacttc acctggaccg
     1921 tgaccggatt gtcgttgtcg cttggcatgt cgatcctgat cttcggcatc gttgtcgcgg
     1981 tgctgttctt cggcacgacg cgcgtgttgt cgctggtcga aggccgcatc gtcgaagtga
     2041 tgctcggcga gcgcatgccg cggcgcccgt tgtacccgcc gcgcgaaggc tcgatgttca
     2101 agcgcatcgg cggcatgttc aaggatccgc gcacctggtc gacgctgctg tacttcgtgc
     2161 tgatgctgcc gctcggaatc gtctatttca cgttcgccgt gactggcatc gcgctgtcgt
     2221 tgtcgttcat cctggcgccg ctcgcgcacc tgggctggat gctgggattg gtgcaggtgg
     2281 actgggccga tcacatccat ttcaacgagc aactggacgt gccctggcag cttgccgatc
     2341 cgctgttgtt cgttctcggc atcgtgctgc tgttcggcct tttgcacctg gcgcgcggca
     2401 tcggcaaatt ccagggcgga ctggccaagg cgctgctggt caattgattg cggagaatcc
     2461 cgtgaccggc gacttccgga aaacgcttga tggcaaaccg ccgcgcatca tcgcgcaccg
     2521 cggtgccagt gggcatcggc cggagcacac gctcgaagcc ttcgccctgg cgctcgcgca
     2581 gggcgccgac attctcgaac cggacttggt cgccagccgc gacggcgtgc tgttcgcgcg
     2641 ccacgatctt ggcctgtcgc gcagcacgga tgtcgccgcg cgtccgcaat tcgcggagcg
     2701 cgcgcgcgaa atcggcggcg agcgcgactg gtggatcg
//
LOCUS       DASRVO010000602         1065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_121958, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000602 DASRVO010000000
VERSION     DASRVO010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121958"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VFF05_07460"
     CDS             complement(<1..1015)
                     /locus_tag="VFF05_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZW51680.1"
                     /translation="MTFSEPVTLSDPWFTIECATSGTHTASVSGGPSAYTLTPAPAFA
                     ANESCTWTILASGIVDNDGTPDHPAADYGVSFTTLDPATQPPTVVSTQPANGAANVPI
                     GSDIRVTFSEAVTTSNAFALSCDSTAIQLDETGSGAARTLTPFTVLPAGKSCVFTITA
                     SGVHDADNIALQADYTASFTIASGTLSGYYSQVNTSSAEQLRCSLHQTIKGHTIYPYS
                     GGTQNTWTILEIAQQDPNDPAKMIDVYRNRSYTAPGDRDGTGSGITYNREHTWPNSLG
                     FADSSLAAYTDTHMLWLSDKTQNADRGNKPYAYCASGCTELTTEANNGFGGGSGTYPG
                     NSNW"
BASE COUNT          161 a          314 c          382 g          208 t
ORIGIN      
        1 accagttgga attgcccgga taggtgccgc tgccgccacc gaagccgtta ttcgcctcgg
       61 tcgtcaattc ggtgcaaccg ctggcgcaat acgcgtacgg cttgttgccg cgatcggcgt
      121 tctgggtttt gtccgacaac cacagcatgt gcgtgtcggt gtaggcggcg agactggagt
      181 cggcgaagcc cagcgaattc ggccaggtgt gctcgcggtt gtaggtgatg cccgaaccgg
      241 tgccgtcgcg atcgccgggg gccgtgtagg agcgattgcg gtacacgtcg atcatcttgg
      301 ccggatcgtt cgggtcctgc tgcgcgattt cgaggatggt ccaggtattc tgcgtaccgc
      361 cgctgtacgg atagatcgtg tggcccttga tcgtctgatg cagcgagcag cgcaactgtt
      421 ccgcactgct ggtgttgacc tgcgagtagt agccggacag cgtgcccgag gcgatggtaa
      481 agcttgcggt gtagtcggcc tgcaacgcta tgttgtccgc gtcgtgcacg ccgctggcgg
      541 taatcgtgaa cacgcaagac ttgccggcgg gcagcacggt gaacggagtc agcgtgcgcg
      601 ccgcgccgct gccggtttcg tcaagctgga tggccgtcga atcgcaggac agcgcgaacg
      661 cgttcgacgt cgttactgcc tcgctgaacg tcacgcggat gtcgctgccg atcggcacgt
      721 tggcggcgcc attggccggc tgcgtggaaa ccacggtcgg cggctgcgtg gccgggtcga
      781 gcgtcgtgaa tgagacgccg tagtcggccg ccggatggtc cggcgtgccg tcgttgtcga
      841 cgatgcctga agcgaggatc gtccaggtgc agctttcgtt cgcggcgaac gcgggcgcgg
      901 gcgtgagcgt gtacgcgctc gggccgccgc tgacgctggc cgtatgcgtg ccggaagtcg
      961 cgcattcgat cgtgaaccac ggatcgctca gcgtgaccgg ctcgctgaaa gtcaccgcca
     1021 gtgttgccga cagcgcgacg ttgcccgcgc cggaacccgg tacgg
//
LOCUS       DASRVO010000603         1968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_121962, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000603 DASRVO010000000
VERSION     DASRVO010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121962"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="VFF05_07465"
     CDS             complement(<1..1809)
                     /locus_tag="VFF05_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZW51681.1"
                     /translation="MAEEKQITFSIDGRQVTTSAGTLVINAAKAQGIEVPSFCYYPGL
                     SLQAACRMCLVEVEKMPKLQTACTLVATEGMIVRTGTPQVHKARKDMIEFLLSNHPLD
                     CPVCDKGGECELRDMTFRYGLDHTRFIEEKLHYPEEKWSPLVYYDAPRCILCFRCVRV
                     CDEGMDVKALGVGARGVQSVILPNTAGALACEECGMCIDICPVGALTSGTYRYQTRPW
                     EMTYVPNPCTHCSNGCKTTLSVRNNEILRANNRDHSGINGEFLCAKGRFGFDFTSHSE
                     RVRQPLVRRNGKLEPASWEEAVEEVARRLKQVHEKHGGNAIGVVGSNHTTNEENYLLN
                     RLARVALGTNNIDHHRTADYAGLAAVLGPKAADSAATMADIYAAQAVLLIANDATQQN
                     PLVAWQIRTGVRHRGQRLYAINGKDSKIHRQATLAVEVPKDAERNALHWLATGDGAFD
                     ANVSAELSNLKATLEKETDVLILFGANVQGVAIRDLVAFGSRLPGRTRYIPLGDYSNS
                     RGAAEMGLLPDRLPGYAPLADKTERARFGKLWGGDIPSTPGVTARAMMDAAVSGKLKA
                     LYVVGANPLKTFAVAAPDRIAGLELLVVQDMFLTETA"
BASE COUNT          325 a          577 c          672 g          394 t
ORIGIN      
        1 cgcggtttcg gtgaggaaca tgtcctgcac gaccagcaat tccaggccgg cgatgcggtc
       61 gggcgcggca acggcgaaag ttttcagcgg attcgcgcca acgacgtaaa gcgccttcag
      121 ttttccggaa acggcagcgt ccatcatggc gcgcgcagtc acgccgggcg tggatggaat
      181 atcgccgccc cacagtttgc cgaagcgcgc gcgttcggtc ttgtcggcga gcggcgcgta
      241 gccgggcaga cggtcgggca ggaggcccat ttcggcggcg ccacgcgagt ttgagtaatc
      301 acccaagggg atgtagcggg tcctgccggg taggcgcgag ccgaaggcga ccaggtcgcg
      361 aatcgccacg ccctggacgt tcgcgccgaa cagaatcaga acatccgttt ccttttcgag
      421 cgtggccttc aggttcgaca gttcggcgga gacgtttgca tcgaacgcgc cgtcgcccgt
      481 cgcgagccag tgcagtgcgt tgcgttcggc atccttcggg acttcgacgg ccagcgtggc
      541 ctggcgatga atcttgctgt ctttcccgtt gatggcgtaa aggcgttggc cgcggtggcg
      601 cacgccggtg cgaatctgcc aggccaccag cggattctgc tgggtggcgt cgttggcgat
      661 cagcaggacg gcttgcgccg cgtagatgtc ggccatcgtc gcggcgctgt cggcggcttt
      721 cgggccgagc acagcggcga ggccggcgta atcggccgtg cggtgatggt cgatgttgtt
      781 cgtgccgagg gcgacgcgcg ccaggcggtt cagcaggtaa ttttcttcgt tggtcgtgtg
      841 attcgagccg accacgccga tggcgttgcc gccgtgcttt tcgtgaacct gcttcaggcg
      901 gcgcgcgacc tcctcgacgg cttcttccca gctggcgggc tcgagcttgc cgttgcggcg
      961 aacgagcggc tggcggacac gttcggaatg agaagtgaaa tcgaaaccga agcggccctt
     1021 ggcgcagagg aactcgccgt tgattccgga atggtcgcga ttattcgcgc gcaggatctc
     1081 gttgttgcgc acgctgagcg tggttttgca gccgttcgag caatgcgtgc agggattggg
     1141 aacgtaggtc atttcccacg ggcgcgtttg atagcgatag gtgccgctgg tgagcgcgcc
     1201 gacggggcag atatcgatgc acatgccgca ttcttcgcag gccagcgcgc cggccgtgtt
     1261 cggcaggatc acggactgca cgccgcgagc gccgacgccc agcgctttga cgtccatgcc
     1321 ttcgtcgcat acgcgcacgc agcggaagca aaggatgcag cggggcgcgt cgtaatagac
     1381 cagcggcgac cacttttctt cggggtaatg cagcttttcc tcgatgaagc gcgtgtgatc
     1441 gaggccgtag cggaaggtca tgtcccgcaa ttcgcactcg ccgcctttgt cgcatacagg
     1501 gcaatcgagc ggatgattcg acagcaggaa ctcgatcatg tccttgcgcg ccttgtggac
     1561 ttgcggcgtg ccggtgcgaa cgatcatgcc ttcggtcgcc accagcgtgc aggcggtctg
     1621 cagcttgggc attttctcga cttcgacgag gcacatgcgg caggcggcct gcagcgacag
     1681 gccggggtaa tagcagaacg agggaacttc gatcccttgc gccttggccg cgttgatgac
     1741 cagcgtgccg gcggaggtgg tgacctgccg gccgtcgatg ctgaacgtga tctgtttttc
     1801 ttccgccata ttttctaaag accgatccgg ctaacggcca acgaggactc cttccgcgga
     1861 tttgtaagcg cacttgccgt gcagatggtc ctcgaactcg ttgcgccatt tcttgacgat
     1921 gctgatcgtg ggcatggccg cggcgtcgcc cagcgggcaa aacgtgcg
//
LOCUS       DASRVO010000604         1022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_226087, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000604 DASRVO010000000
VERSION     DASRVO010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226087"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>1022
                     /locus_tag="VFF05_07470"
     CDS             13..>1022
                     /locus_tag="VFF05_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51682.1"
                     /translation="MSEPRADRPVVVGFGPCGIFAALVLAQMGLRPIVLERGKKVRER
                     TRDTWGLWRRRELNPESNVQFGEGGAGTFSDGKLYSQIKDPRHLGRKVLEEFVKAGAP
                     AEILYESHPHIGTFRLVGMVENMRETIRSLGGEIRFGSRVDDIELGNGAVCGVVLAGG
                     EHIATNHLVLAVGHSARDTFEMLHKRGVFMEPKPFSIGFRIEHPQPLIDRARFGSAAG
                     NPLLGAADYKLVHHAKNGRSVYSFCMCPGGTVVAAASEPGRVVTNGMSQYSRNERNAN
                     SGIVVGITPEDYPGGPLAGIELQRKLEARAFELGGGDYEAPGQLVGDFIEGKASTDLG
                     SV"
BASE COUNT          180 a          317 c          361 g          164 t
ORIGIN      
        1 ccgccggtgc acgtgagcga gccgcgggcc gaccgcccgg tcgtcgtcgg cttcggtccc
       61 tgcggcatct tcgcggcgct ggtgctcgcc cagatgggtc tgcggccgat cgtgctcgag
      121 cgtggcaaga aagtgcgcga acgcaccagg gacacctggg gcctgtggcg caggcgcgag
      181 ttgaacccgg agagcaacgt gcagttcggc gaaggcggcg ccggcacgtt ctcggacggc
      241 aagctgtaca gccagatcaa ggatccgcgc cacctggggc gaaaagtgct ggaggaattc
      301 gtcaaggcgg gcgcacccgc agagattttg tacgagagcc atccgcacat cggcacgttc
      361 cgcctggtcg gcatggtcga aaacatgcgc gagacgatac ggtcgctggg cggcgagata
      421 cgtttcggga gtcgcgtcga cgacatcgaa ctgggaaatg gcgccgtgtg cggcgtagtg
      481 cttgccggcg gcgagcatat cgcgacgaat catctggtgc tggcagtcgg gcacagcgcg
      541 cgcgacacct tcgagatgct gcacaagcgc ggcgtgttca tggagcccaa gccattctcc
      601 attggttttc gcatcgaaca tccgcaaccg ctcatcgacc gcgcgcgttt cggctccgcc
      661 gccggcaatc cgctgctggg cgcggcggac tacaagctgg tgcaccacgc caagaacggc
      721 cgttcggtgt acagcttctg catgtgcccc ggagggacgg tggtcgcggc cgcctccgaa
      781 ccggggcgcg tggtcaccaa cggcatgagc cagtattcgc gcaacgagcg caacgccaac
      841 agcggcatcg tcgtcggcat cacgcccgag gattatcccg gcgggccgct tgccggcatc
      901 gaactgcagc gcaagctgga ggcgcgtgcg ttcgaattgg gcggtggcga ttacgaggcg
      961 cctggccagc tggtcgggga tttcatcgag ggaaaggcct cgacggatct cggcagcgtc
     1021 ga
//
LOCUS       DASRVO010000605         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_121994, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000605 DASRVO010000000
VERSION     DASRVO010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121994"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFF05_07475"
     CDS             complement(<1..464)
                     /locus_tag="VFF05_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002804535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZW51683.1"
                     /translation="MNPFDPRQIRRAFGRAAKTYTRNAALQRKIEARLIERLEYFSGK
                     PARVLDVGCGPGHGSALLRKRFRSAQVIALDLALPMLRQIRPGWRRPLGRVCADARAL
                     PLPDASVDVLFSNLCIQWIANLPALFAEFRRVLKPGGYLAFSTFGPDTLHELR"
     gene            complement(461..1225)
                     /gene="bioH"
                     /locus_tag="VFF05_07480"
     CDS             complement(461..1225)
                     /gene="bioH"
                     /locus_tag="VFF05_07480"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HZW51684.1"
                     /translation="MHIETSGDGPDMVLIHGWAMHGGVFATLARALRERFRLHVVDLP
                     GHGFSRDDDSRLDPESCAKNLLDTLPRALWIGWSFGGLVSLHAALIQPEQLRGIVQVA
                     TSPRFVAAPDWPHGVALDVFAQFESGLRRDYRATIERFLALEAIGSDDAQAELRDLKA
                     QVFSRGEPAVAALECGMQILETTDLRARLHELAVPNLWIAGRRDRLVPAAAMHWAAQQ
                     SPQRNFVEISSGHAPFVSHAAEVADTIAAFAENLAA"
     gene            complement(1227..>1922)
                     /locus_tag="VFF05_07485"
     CDS             complement(1227..>1922)
                     /locus_tag="VFF05_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZW51685.1"
                     /translation="RQLDARPNAAAILATDAVFSMDGDVAPLAALSALCRDEHATLMV
                     DDAHGIGVLGADGAGSLSEARLAQDDAPVLMATLGKALGVAGAFVAGSAALIDGLVQF
                     ARSYVYTTAMPPALAAAASAAVDIARDDGDRRAHLHRNIAQFREGSAKRSISLLASTT
                     PIQPIPTGDSATALAAAQRLEDAGFYVPAIRPPSVPANGARLRVSLSAAHAQADIERL
                     LDALVIAFAPERH"
BASE COUNT          336 a          612 c          679 g          295 t
ORIGIN      
        1 cgtagttcgt gcagggtgtc cggcccgaac gtggaaaacg cgaggtagcc gcccggcttg
       61 agtacgcgcc ggaattcggc gaacagcgcc ggcaggttgg cgatccactg gatgcaaaga
      121 ttggaaaaca gcacgtccac gctcgcatcc ggcagcggca acgcgcgcgc gtccgcgcac
      181 acgcggccga gcggacgccg ccagccggga cggatctggc gcagcatggg caaggcgagg
      241 tcgagcgcga tcacttgcgc cgaacggaat cgcttgcgca gcaatgcgct gccgtgaccg
      301 ggcccgcagc cgacatcgag gacgcgcgcc ggcttgccgg aaaaatattc cagccgttcg
      361 atcaggcgcg cttcgatctt gcgctgcagg gctgcgttgc gcgtgtaggt tttcgcggcg
      421 cggccgaagg cacggcggat ctggcggggg tcgaacgggt tcatgcggca aggttctcgg
      481 cgaaagcggc gatggtatcc gcgacttccg cggcgtgcga aacgaagggc gcatggcccg
      541 aggaaatttc cacaaaattg cgctgtggac tttgctgcgc ggcccagtgc atggcggcgg
      601 cgggaacgag tcggtcgcgg cgtccggcga tccacaggtt gggtacggca agttcgtgca
      661 ggcgcgcgcg cagatcggtg gtttccagta tctgcatgcc gcattccagc gctgcgacgg
      721 ccggctcacc gcgcgaaaaa acctgagcct tcagatcgcg caattcggcc tgggcgtcgt
      781 ccgagccgat cgcctccagc gcgagaaagc gctcgatggt ggcgcggtaa tcccgacgca
      841 gtccggattc gaactgcgcg aacacgtcca gcgccacgcc atgcggccaa tccggcgcgg
      901 cgacgaaacg cggcgacgtg gcgacctgca cgatgccgcg caactgctcg ggctgaatca
      961 gcgccgcgtg cagagacacg agcccgccga acgaccagcc gatccagagg gcgcgtggca
     1021 gcgtgtcgag gagattcttc gcgcaggatt ccggatcgag tctgctgtcg tcatcgcggc
     1081 tgaaaccatg gccgggtaga tcgaccacgt gcagacggaa tcgttcgcgc agcgctcgcg
     1141 cgagcgtcgc gaatacgccg ccatgcatgg cccagccatg gatcaggacc atgtccggac
     1201 cgtcgcccga ggtttcgatg tgcatgtcag tgccgctccg gcgcgaacgc gataacaagc
     1261 gcatcgagca ggcgttcgat gtcggcctgc gcatgcgcgg cggacaggct cacacgcagc
     1321 cgtgcaccgt tggcgggcac gctcggcggg cggatcgcgg gcacatagaa acccgcgtct
     1381 tccaaacgct gtgcggcggc gagtgcagtt gcgctgtcgc cggtcgggat tggctggatc
     1441 ggcgtcgtcg aggcgagcag tgaaatgctg cgttttgcgg aaccttcgcg aaattgtgca
     1501 atgttgcgat gcaggtgtgc ccggcggtcg ccgtcgtcac gcgctatgtc cacggccgca
     1561 ctggcggcgg cagcaagcgc cggcggcatg gccgtggtgt agacgtaact gcgcgcgaat
     1621 tgcacgagtc cgtcgatgag cgccgcgctg ccggcgacaa aggcaccggc cacgccgagc
     1681 gccttgccca gcgttgccat cagcacaggg gcatcgtcct gtgcgagtcg ggcttcgctg
     1741 aggctgcccg cgccatcggc accaagcacg ccgatgccgt gcgcatcgtc gaccatcaag
     1801 gtcgcgtgct cgtccctgca caaggccgac agcgccgcga gcggcgcgac gtcgccgtcc
     1861 atgctgaaca cggcatcggt ggcgaggatc gccgcagcat ttggtcgcgc gtcaagctgg
     1921 cg
//
LOCUS       DASRVO010000606         2538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_44665, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000606 DASRVO010000000
VERSION     DASRVO010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44665"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..2534)
                     /gene="mgtA"
                     /locus_tag="VFF05_07490"
     CDS             complement(3..2534)
                     /gene="mgtA"
                     /locus_tag="VFF05_07490"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HZW51686.1"
                     /translation="MLLQSLDANLEGLSSAEAASRRLRYGANQPRDAAHKGIVLTLAG
                     LFANPLVVILLLAALVSGFLGDRVGATIIVAIVTLSIMLNFALSFRSERAATRLREEV
                     APMATVCRDGGWTDLPRRELVPGDIIRLSAGDRVPADARLIESRDLHVQQAALTGESV
                     PAEKEAAVQGRSERGPEAPHLVFLGTSVVSGHATALVVATGRATAFGDVAEMLAARPP
                     PTEFERGLDGFARLIMRTVVFLVLFVLLIGIALGRPPLECLLFALALAVGLTPEFMPM
                     ITTMTLAKGAVRMARRHVIVKHLAAIEDFGSMTVLLSDKTGTLTYGETTVAGVFDPLG
                     NPADRPLRMAYLNSTFETGIRSPFDDAILRHGAVDIAGYRKLDEIPFDFERRRLCIVV
                     ERQDERLLIVKGAPEAVMAECAQCECEGGVRAMDAAMRVQCLRAHEALGEQGLRVLAV
                     AWRRVPVQARYAATDESDLILAGFLAFADPIAPDVAGALAALAADGVAVKVLSGDNER
                     VARHVCAQAGIDVANTVLGSEIDDLSETALGILAERTAVFARVSPAQKHRILLALKAR
                     GHVIGFLGDGINDAPSLHAADVGISVANAVDVARDAADIVLRERDLRILHAGVIEGRK
                     AFANVMKYLLMGTSSNFGNMFSMAAGMLFLPFLPMLPTQILLNNFLYDLAQTTIPTDN
                     VDAAQLRLPTRWNVAVIRRFMLGVGPISSLFDFLTFFVLLHWLQASPVLFHTGWFVES
                     LATQTLVLFVIRTRGNPLRSRPSATLVATILAAVAVGVAIPATGLGQDLGFTPLPPAF
                     FAFLAVATATYLLLVELAKRRFMGAMVAKSRYELTSINRRQAKRS"
BASE COUNT          452 a          819 c          880 g          387 t
ORIGIN      
        1 cgctagcttc gttttgcctg ccgcctgttg atggatgtca attcgtagcg ggatttcgcg
       61 accatagcgc ccatgaaacg ccgcttggca agttcgacca gcaacaggta ggtggccgtg
      121 gctaccgcca ggaatgcaaa aaatgccgga ggcagtggcg tgaacccgag gtcctgcccc
      181 aacccggttg ccggaatcgc cacgccgacg gcaacggcgg caaggatcgt cgccacgagc
      241 gtggcactgg gccggctgcg cagcgggttg ccgcgggtcc ggatcacgaa cagcaccagc
      301 gtttgcgtgg ccagcgactc gacgaaccag cccgtgtgga acagcaccgg cgacgcctgc
      361 agccaatgca gcaacacgaa gaaagtcagg aaatcgaata gcgaactgat gggtccgacg
      421 cccagcatga agcgccggat cacggcgaca ttccagcgcg tcgggaggcg gagctgcgcg
      481 gcatcgacgt tgtcggtcgg gatcgtggtc tgggcgagat cgtagaggaa attgttgagc
      541 aggatttgcg tcggcagcat gggcaggaac ggcaggaaca gcatgccggc ggccatgctg
      601 aacatgttgc cgaaattcga gctggttccc atcagcaggt acttcatgac gttcgcgaac
      661 gccttgcggc cctcgatcac gccggcatgc aggatgcgca ggtcgcgctc gcgcagcacg
      721 atgtcggcgg catccctggc cacgtccacg gcattggcga ccgaaatgcc gacatcggcc
      781 gcgtgcaggg acggcgcgtc gttgatgccg tcgccgagga aaccgatgac gtggccgcgc
      841 gccttcaacg cgagcaggat gcgatgcttc tgcgcgggcg aaacgcgcgc gaacacggcg
      901 gtgcgttcgg ccaggatgcc gagcgcggtc tcgctcaggt cgtcgatctc gctgcccagc
      961 accgtgttcg cgacgtcgat gccggcctgt gcgcatacgt gccttgccac gcgctcgttg
     1021 tcgccgctga ggaccttcac ggccacgccg tcggcagcca gcgccgccag tgcgccggcg
     1081 acgtcgggcg cgatcggatc ggcgaaggcg aggaagccgg cgaggatcag gtcgctttcg
     1141 tcggtcgccg cgtagcgcgc ttgcaccggg acgcggcgcc aggcgacggc gagcacgcgc
     1201 agcccctgtt cgccgagcgc ctcgtgtgca cgcaggcatt gcacacgcat cgcggcgtcc
     1261 atcgcgcgca cgccgccttc gcattcgcat tgcgcgcact cggccatgac tgcctccggt
     1321 gcgcccttga cgatcagcag tcgctcgtcc tggcgctcca cgacgatgca caggcggcgg
     1381 cgctcgaagt cgaacggaat ctcgtcgagc ttgcggtagc cggcgatgtc gaccgcgcca
     1441 tggcgcagga tcgcatcgtc gaacgggctg cggatgccgg tttcgaacgt gctgttgagg
     1501 taagccatcc gcaaggggcg atccgccgga tttccgagcg gatcgaacac gccggcgacc
     1561 gtggtctcgc cgtacgtcag cgtgccggtc ttgtcgctga gcaacaccgt catgctgccg
     1621 aaatcctcga tcgcggccaa atgtttgacg atcacatgcc ggcgcgccat gcgcaccgcg
     1681 cccttggcga gcgtcatggt cgtgatcatc ggcatgaatt cgggggtgag tccgaccgcg
     1741 agcgcgagcg caaacagcag gcattccagc ggcgggcgac cgagtgcgat gccgatgagc
     1801 aggacgaaca gcacgaggaa cacgacggtg cgcatgatca ggcgcgcgaa accatcgagg
     1861 ccgcgctcga attcggtcgg cggcgggcgc gcggcgagca tctcggcgac atcgccgaat
     1921 gccgtggcgc ggccggtcgc cacaaccagc gccgtcgcat ggccggacac cactgaggtg
     1981 cctaggaaaa cgaggtgagg cgcctcggga ccgcgctcgc tgcggccttg cactgcagcc
     2041 tccttttccg ctggcacgga ttctccggtc agtgccgcct gctgcacgtg caggtcgcgc
     2101 gattcgatca ggcgcgcatc cgccggcacg cgatcgccgg cggacaaccg gatgatgtct
     2161 ccgggaacca gttcgcgccg tggcagatcc gtccagccac catcgcggca gaccgtcgcc
     2221 atcggcgcga cttcctcgcg caggcgcgtg gctgcacgtt cggaacgaaa gctcagggcg
     2281 aagttgagca tgatgctcag ggtgacgatc gcgacgatga tcgtcgcgcc cacgcggtcg
     2341 ccgaggaaac cggaaaccag cgcggcgagc agcaggatga cgaccaaggg gttggcaaac
     2401 aggccggcga gggtgagcac gattcccttg tgcgcggcgt cgcggggctg gttggcgccg
     2461 tagcgcaggc gccgcgaggc cgcctcggca ctcgacaagc cctcgagatt tgcgtcgagg
     2521 gattgcagga gcacgccg
//
LOCUS       DASRVO010000607         1161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_354870, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000607 DASRVO010000000
VERSION     DASRVO010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354870"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFF05_07495"
     CDS             <1..600
                     /locus_tag="VFF05_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51687.1"
                     /translation="DPHFIVMLRSPVEIAHSLHNEYAKHDDEQTDFEACWRLQASRLQ
                     GRDLPARFSDGAALQYGEIAKTGAQLERLLGLVERRQVHVIVYDDFSANPAASYAAVL
                     DWLGLEHDGQAQFEKINPSVTYQWPGLEYALRKIRSVRSALGLPGGLGIQALIDRFNK
                     KHVREPLRPAFRQELCDYFRDDVALLSRLLGRDLSHWLD"
     gene            644..>1161
                     /locus_tag="VFF05_07500"
     CDS             644..>1161
                     /locus_tag="VFF05_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZW51688.1"
                     /translation="MENAITAGANPLKAIRNKSDLAIHGAPPAFAEPVHVGRPNIGNR
                     AAFLARVGQVLDNHWLTNNGPMVQEFEARIAQFLGVKHCVAMCNGTIALEIAIRALGL
                     EGEVIVPSWTFVATAHALYWQGITPVFADIDPATHNLDPDAVRRMITPRTSGIIGVHL
                     WGRAAPVDELQA"
BASE COUNT          218 a          394 c          363 g          186 t
ORIGIN      
        1 gatccgcact tcatcgtgat gctgaggagc cccgtcgaaa ttgcgcatag cctgcacaac
       61 gaatatgcga aacacgacga cgaacaaact gactttgaag cttgttggcg actacaagca
      121 tcgcgattgc agggccgcga cctgccggcc cgattcagcg acggtgccgc cctgcagtac
      181 ggcgagatcg cgaaaaccgg cgcgcagctc gaacgcttgc ttggcctcgt tgaacgccgg
      241 caggtgcatg tcatcgtgta cgacgatttc agtgcgaatc ccgccgccag ctatgccgcc
      301 gtgctggact ggctggggct ggaacacgac ggccaggcac agttcgagaa gatcaatccc
      361 agcgtcacct accagtggcc cggtctcgag tacgccctgc gcaagatccg cagcgtgcgc
      421 agcgcgctgg gcctgccggg cgggctgggc atccaagccc tgatcgaccg cttcaacaag
      481 aagcacgtgc gcgaaccgtt gcgccccgca ttccggcaag agctgtgcga ttacttccgc
      541 gacgacgtcg ccctgctgtc gcggctgctc gggcgcgacc tgtcgcactg gctggattga
      601 gcgcgcgccg cgcggccgcg gcggtttggg ttacgctagc gacatggaaa acgcgatcac
      661 cgccggagca aatccgctga aagccatccg caacaaatcc gacctcgcca tccacggcgc
      721 gccaccggcc tttgccgaac cggtgcacgt gggccggccc aacatcggca accgcgcggc
      781 gtttctcgcc cgcgtcggcc aggtcctcga caaccactgg ctgaccaaca acggcccgat
      841 ggtgcaggag ttcgaggcgc gcatcgcgca atttctcggc gtgaagcact gcgtggcgat
      901 gtgcaacggc accatcgcgc tggaaatcgc gatccgcgcg ctggggctgg aaggcgaagt
      961 catcgtgccg tcgtggacct tcgtagccac cgcgcacgcg ctgtattggc aaggcatcac
     1021 gccggttttc gccgatatcg acccagccac gcacaacctc gatccggatg ccgtgcggcg
     1081 catgatcacg ccgcgcacca gcggcatcat cggcgtgcac ctgtggggcc gcgccgcgcc
     1141 ggtcgacgaa ttgcaggcca t
//
LOCUS       DASRVO010000608         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_172863, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000608 DASRVO010000000
VERSION     DASRVO010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172863"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFF05_07505"
     CDS             complement(<1..189)
                     /locus_tag="VFF05_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZW51689.1"
                     /translation="MFVRLLFLLLLALNFGAAAWLFIAPQPQARAFAPTDSGVAELVL
                     LSERDRGSEASAAELASAP"
     gene            complement(261..992)
                     /locus_tag="VFF05_07510"
     CDS             complement(261..992)
                     /locus_tag="VFF05_07510"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZW51690.1"
                     /translation="MKLLVDAGNSRLKWAWWDGAALGDVAATANAEMDIAALWKDGQI
                     ADAVWVASVASAAANTALAHALRERFGIEPVFAATRAQACGVRIAYAQPANFGVDRFL
                     GLIAAHAQTQAASVVASCGTALTLDALAADGTHLGGLIAAGPQLAQNALRGGTAALAT
                     VPVGRVVEIADNSADAVESGTWLSAAALTERFLAQAGRRLGSAPKLILTGGGAVRLSP
                     LLDVVHRIDARLVLRGLAMLADARD"
BASE COUNT          197 a          403 c          339 g          148 t
ORIGIN      
        1 cggcgcactc gccagttccg ccgcgctggc ttcgctgccg cggtcgcgct cggacagcag
       61 cacgagttcg gccacgcctg aatcggtcgg cgcgaacgca cgcgcctgcg gctgcggcgc
      121 gatgaacaac caggccgccg cgccaaaatt cagcgccagc agcagcagga acaacaaacg
      181 aacgaacatc gggattccaa cggcagcgca tccgccgcct cgcaacgcgg gattctagcc
      241 gaggccacgg cttcgtgcgc tcagtcccgc gcgtcggcca gcatcgccag tccgcgcaac
      301 acgagccgcg catcgatgcg atgcactaca tcgagcaatg gcgaaagtcg caccgcgccg
      361 ccgccggtca ggatcagttt cggtgcgctg cccagccgcc gtccagcctg cgccagaaaa
      421 cgttcggtca aggccgccgc cgacagccag gttccggatt cgaccgcatc ggcgctgttg
      481 tcggcgattt ccacgacacg gcccacgggc acggtggcca aagctgcagt gccgccgcgc
      541 aacgcgttct gcgccaactg cggacccgca gcgataagcc cgcccagatg ggtgccgtcg
      601 gcggcaagcg cgtccagggt cagggccgtg ccgcaactgg cgaccacgct cgccgcctgc
      661 gtctgcgcat gcgcggcgat caaaccgagg aaacggtcca cgccgaaatt tgccggttgc
      721 gcgtaggcaa tgcgcacgcc gcaggcctgc gcgcgcgtcg ccgcaaacac gggttcgatg
      781 ccgaagcgtt cgcgcagtgc atgcgccaac gccgtgttcg ccgcggcgga agccaccgaa
      841 gcgacccaga ccgcgtcggc aatttgtcca tctttccaca atgcagcaat gtccatttcg
      901 gcattcgccg tcgcggctac gtcgcccaac gcggcgccgt cccaccaggc ccacttcagg
      961 cgcgaattgc cggcgtcgac gagtagtttc atgcagcgcg caccgacacg tcgacgctgt
     1021 cgtaaacggc cagggtcgca ccgtggcgca cacgcaacgc gccgcgctcg tccacgccct
     1081 cggcaat
//
LOCUS       DASRVO010000609         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_354906, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000609 DASRVO010000000
VERSION     DASRVO010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354906"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..82
                     /locus_tag="VFF05_07515"
     CDS             <1..82
                     /locus_tag="VFF05_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HZW51691.1"
                     /translation="LSEAIGKLGVGNRIEAYRLARQKGWL"
     gene            219..1256
                     /gene="queA"
                     /locus_tag="VFF05_07520"
     CDS             219..1256
                     /gene="queA"
                     /locus_tag="VFF05_07520"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZW51692.1"
                     /translation="MKKSDFHYDLPPELIAQAPLAQRSASRLLVVNVPEKRFADKQFT
                     DLTGFLRDGDLLVFNDTRVLPARLYGKKETGGAVEILIERVTGAHEAVAQIGVSKKPK
                     AGSRILLEDSSALEVLGREGEFYTLRHKGPEALEKLLLRLGHMPLPPYIARADEAADA
                     ERYQTVYARAPGAVAAPTAGLHFDAPLLDALRAKGVDFGYVTLHVGAGTFQPLRSERV
                     EDHTMHREWLNVGAELVEKMRHARARGGRVIAVGTTVVRALESALRDGQVQPFAGETQ
                     IFIFPGYRIASVDALITNFHLPESTLLMLVSAFAGRELMLDAYRHAVEQRYRFFSYGD
                     AMLILPKGANE"
     gene            1253..>1986
                     /gene="tgt"
                     /locus_tag="VFF05_07525"
     CDS             1253..>1986
                     /gene="tgt"
                     /locus_tag="VFF05_07525"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZW51693.1"
                     /translation="MSSMQFQLLATDGAARRGRVRFPRGTIETPAFMPVGTYGSVKGM
                     TPRSVAETGAQIILGNTFHLWLRPGLDVIGEFGGLHKFIGWNKPILTDSGGFQVFSLA
                     ERRKITEEGVRFASPVDGSRVFLSPEESMRIQRVLDSDIAMIFDECTPYPATERQVRD
                     SMELSLRWAARSRHAFDELRNPNALFGIVQGGTYPQWREVSARGLLQIGFDGYAVGGL
                     AVGEPEAERNRTLEETVPLLPADRPR"
BASE COUNT          349 a          615 c          659 g          363 t
ORIGIN      
        1 cctgtccgag gcgatcggca agctcggcgt gggtaaccgg atcgaggcgt atcggctggc
       61 aaggcagaag gggtggttgt gatctgttgc gcctgcgcga tgccttgaat ggcttcgcgc
      121 acagcggatc atcggtgagc caagaatggc gaacggagga gatccaggga tggctgcttg
      181 gcttcgaccg agcaacgcgc tccgcgtaca atcgcgcggt gaaaaaatcc gacttccatt
      241 acgatctgcc gccggaattg atcgcgcagg cgcctctcgc gcagcgttcg gcgagccgcc
      301 tgcttgtggt caatgtgccg gaaaaacgct tcgccgacaa gcagttcacc gacttgaccg
      361 gattcctgcg cgacggcgac ctgctcgtat tcaacgacac gcgcgtgctg ccggcgcgct
      421 tgtacggcaa gaaggaaacc ggcggcgcgg tggaaatcct gatcgagcgc gtcaccggcg
      481 cgcatgaagc tgtcgcgcag atcggcgtga gcaagaagcc gaaggcgggc agcaggattc
      541 tgctcgaaga ctccagcgcg ctcgaagtgc tcggtcgcga aggggaattt tatactttgc
      601 gtcacaaagg ccccgaggcg ctggaaaaac tcctgctgcg tctgggccac atgccgctgc
      661 cgccgtatat cgcgcgcgcc gacgaagccg cggacgccga acgctatcaa accgtttatg
      721 ccagggcgcc cggtgcggtc gcggcgccga ccgccggttt gcacttcgat gcgccactgc
      781 tcgacgcgct gcgcgccaag ggcgtggatt tcggttacgt caccctgcat gtcggtgcgg
      841 gcacgttcca gccgctgcgc agcgaacgcg tcgaagacca caccatgcac cgcgaatggc
      901 tcaatgtcgg tgcggaactc gtcgagaaga tgcggcatgc gcgggcgcgc ggcgggcgcg
      961 tgatcgcggt cggcaccacc gtggtgcgcg cgttggaatc ggcgctacga gatgggcagg
     1021 tgcagccatt cgcaggcgag acgcagattt tcatcttccc cggctaccgc atcgccagcg
     1081 tcgacgcgct gatcacgaat ttccacctgc ccgaatcgac gttgctgatg ctggtctcgg
     1141 ccttcgccgg acgcgagttg atgctcgatg cgtatcgcca cgccgttgaa caacgctacc
     1201 ggtttttctc gtatggagat gccatgctga tcttgccgaa aggcgccaac gaatgagttc
     1261 gatgcaattc cagttgcttg ccaccgatgg cgctgcgcgc cgcggtcggg tgaggtttcc
     1321 gcgcgggacg atagaaacgc cggcattcat gccggtgggc acctatggat cggtcaaggg
     1381 catgacgccg cgttcggtcg ccgagaccgg tgcgcagatc atccttggta acaccttcca
     1441 tctgtggctg cgaccgggcc tcgatgtcat cggcgagttc ggcgggctgc acaagttcat
     1501 cggctggaac aaacccatcc tcaccgattc cggcggtttc caggtcttct cgctggccga
     1561 gcgacgcaag atcacggagg agggcgtgcg cttcgcctcg ccggtggacg gttcgcgcgt
     1621 gttcctgtcg ccggaggaat ccatgcgcat ccagcgcgtg ctcgattccg acatcgccat
     1681 gatcttcgac gaatgcacac cgtatcccgc gactgaacgg caggtgcgcg attcgatgga
     1741 gctcagcctg cgctgggccg cgcgctcgcg ccatgcgttc gacgaattac gcaatccgaa
     1801 cgcgcttttc ggcatcgtgc agggcggcac gtatccgcag tggcgcgagg tttcggcgcg
     1861 cgggctgctg caaatcggct tcgacggcta cgcggtcggc ggccttgcgg tcggcgaacc
     1921 cgaggccgag cgcaaccgca cgctggagga aacggttccg ctgttgccgg cggatcgccc
     1981 gcgtta
//
LOCUS       DASRVO010000610         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_381155, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000610 DASRVO010000000
VERSION     DASRVO010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381155"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..2360
                     /locus_tag="VFF05_07530"
     CDS             9..2360
                     /locus_tag="VFF05_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZW51694.1"
                     /translation="MEQAAGPGALSRLLDPRTLLFLGACLAFVAGAFSAWQAWLMWSR
                     ISGAQQIDTVTHAELAALNKFVSDTRMRVIREIDSEAVVAALATPGDVEHAAAVAVLK
                     QALPDLVSADFYRPDLSDVLGTDFAKFGYAKAAMLVQAHQTQIGAPLQSHQAGRNRRI
                     LAFALPVLHDKQTLAYAYVELPFEPLLKVFQEQRLSDARIDLRQGEGRGDLVLASIGS
                     TTAHDSLTDLGLGVGGSLFRIGGTAEDFSIFLPGGLWLAVPLTLILFGLGLFLLQARD
                     IGLREALDRFLRRRSTVPAEAEQTLAQSVAKPAAAPKPKPAQAAAQPDVAVDRSIFRA
                     YDIRGVLGKTLTAGVARQIGRAIGSEAQQRGLKEIVVGRDGRLSGPELAAALIEGLRA
                     TGCNVIDIGAVPTPVLYFATYHLNAGSGVMVTGSHNPPDYNGFKIVLGGETLAEQAIA
                     DLYVHISENRLSSGSGGLQTMDVTSDYIERITGDVLIERKLKVVVDCGNGIPGAVAPT
                     VLEGIGCEVQPLYCDVDGNFPNHHPDPSDPHNLQDLILSVKQMHADVGLAFDGDGDRL
                     GVVTANGEVIFPDRTLMLFARDVLTRNPGATIIYDVKCTGKLQPWILQAGGSPIMWKT
                     GHSLIKSKMRETDAELAGEMSGHFFFRERWYGFDDGIYAAARLMEILAADPEGRAPQE
                     IFDDLPKGVSTPELKLEMPEGEHYRFIEKFKEKSSFEGARVTTIDGVRADWPDGWGLV
                     RASNTTPVLVLRFDADTDVALARIQDVFRKQLLALDANLKLPF"
BASE COUNT          407 a          783 c          776 g          416 t
ORIGIN      
        1 aacgcacgct ggaacaggcg gccgggcccg gtgcgctgtc gcgcctgctc gatccacgca
       61 cgctgctgtt cctcggcgcc tgccttgcct ttgtcgccgg cgccttcagc gcatggcagg
      121 catggctgat gtggagccgg atcagcggcg cgcagcagat cgacacggta acgcatgccg
      181 agcttgcggc actgaacaaa ttcgtctcgg acacccgcat gcgcgtgatt cgcgaaatcg
      241 acagcgaagc cgttgtcgcg gcgttggcca cgccgggcga cgtcgagcat gccgccgccg
      301 tggctgtact caagcaggcg ctgcccgatc tcgtttccgc ggatttctac cgcccggacc
      361 tgagcgacgt gctcggcacc gatttcgcca agttcggcta cgccaaggcg gcgatgctgg
      421 tgcaggcgca ccagacccag atcggcgcac cgctgcagtc gcatcaggcc gggcgcaacc
      481 gccgcatcct cgccttcgct ctgccggtgt tgcacgacaa gcagacgctg gcgtatgcct
      541 acgtcgagtt gccgttcgag ccgttgctca aggtgttcca ggagcagcgt ctgtcggatg
      601 cgcgcatcga cctgcgccag ggcgaaggcc gcggcgacct ggtgctggcc agcatcggca
      661 gcacgacagc gcacgattcg ctgaccgacc ttggtcttgg tgtcggtggc agcctgtttc
      721 gcatcggcgg gacggcggaa gatttttcga tctttcttcc cggggggctg tggctggcgg
      781 ttccgctcac tttaatcctg ttcggtctcg gcctgttcct gttgcaggca cgcgatatcg
      841 gtctgcgcga ggcgctggat cgctttctgc gccgccgatc gaccgtgccg gccgaggcag
      901 agcagacgtt ggcgcaaagt gttgccaagc cagccgcggc gcccaagccc aagcccgcgc
      961 aagcggcggc gcaacccgat gtcgccgtcg accgcagcat cttccgcgcc tacgatatcc
     1021 gcggcgttct cggcaagacg ctgaccgccg gcgtggcgcg ccagatcggc cgtgccattg
     1081 gcagcgaggc gcagcagcgc ggcttgaagg aaatcgtggt cggccgcgac gggcgcttgt
     1141 ccggcccgga gcttgccgcc gcgctgatcg agggcctgcg cgcgactggc tgcaacgtca
     1201 tcgacatcgg cgcggtgccg acgccggtgc tgtacttcgc cacctatcac ctcaatgccg
     1261 gttccggcgt gatggtcacc ggcagccata atccgccgga ctacaacggt ttcaagatcg
     1321 tgctcggcgg cgaaaccctg gccgagcagg cgatcgccga tctctatgtg cacatcagcg
     1381 aaaaccgcct cagcagcggc agcggtggcc tgcagacgat ggacgtcacg tccgactaca
     1441 tcgagcgcat caccggcgac gtgctgatcg agcgcaagct caaagtcgtg gtcgactgcg
     1501 gcaacggcat tcccggcgcg gttgcgccga cggtactcga aggcatcggc tgcgaggtgc
     1561 agccgctgta ttgcgacgtc gacggcaatt tcccgaacca ccatccggac ccgtccgatc
     1621 cgcacaacct gcaggacctg atcctgtcgg tgaagcagat gcacgcggat gtcggcctcg
     1681 ctttcgacgg cgacggcgac cgtctcggcg tggtaaccgc gaacggcgag gtaatcttcc
     1741 ccgatcgcac gctcatgctg tttgcgcgcg atgtgcttac gcgcaatccc ggcgcgacga
     1801 tcatctacga cgtcaaatgc accggcaaat tgcagccgtg gatcctgcaa gccggcggca
     1861 gcccgatcat gtggaagacg ggacattctc tgatcaagtc gaagatgcgc gaaaccgatg
     1921 ccgaactcgc cggtgagatg agcgggcatt tcttcttccg cgagcgctgg tacggcttcg
     1981 acgacggcat ctacgccgcc gcgcggctga tggaaatcct cgccgccgat ccggaagggc
     2041 gcgcgccgca ggagatcttc gacgacctgc ccaagggggt gtccacgccg gaactgaagc
     2101 tcgagatgcc cgaaggcgag cactaccgct tcatcgagaa gttcaaggaa aagtcgagct
     2161 tcgaaggcgc gcgtgtcacc accatcgacg gcgtgcgcgc ggactggccg gatggctggg
     2221 gcctggtgcg cgcctcaaac acgacgccgg tgctagtgct gcgtttcgac gccgataccg
     2281 atgtcgcgct ggcacgcatc caggatgtgt tccgcaagca gttgctggcc ttggacgcga
     2341 acctgaagct gccgttctag ggcacctgcg aacaacctgt tg
//
LOCUS       DASRVO010000611         5694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_44938, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000611 DASRVO010000000
VERSION     DASRVO010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44938"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFF05_07535"
     CDS             <1..967
                     /locus_tag="VFF05_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51695.1"
                     /translation="SRLSSPWRLDTGDDAELLLIDVDTVYGHMDWLRAHNSRLVAVLT
                     GHTPGDEYEMVLHKPLTVDNLVQVLDRAAALTNEGPADAPAPPPAPAPKKTVAKAAPA
                     PKPVAAATPVAAPAPEPPPVPEPPKERRLHHWLADHALAAAVRVHSKDAPDLVLDPGN
                     KTYYADGNLRALAAHCGRVVAHEELHEVSPIELARLQASGKGQPYARLNWLSHVLGSN
                     GHPTQGVDVNAKFKMSRWPQIEREFPKHFRIATVMMKQPATLGEIAEQSGATLPDVID
                     FTNAYHAIGFIEIERTAAAAEAAAPRDTGRGAILSRLKNPFGSGT"
     gene            998..1756
                     /gene="ygiD"
                     /locus_tag="VFF05_07540"
     CDS             998..1756
                     /gene="ygiD"
                     /locus_tag="VFF05_07540"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HZW51696.1"
                     /translation="MPALFVGHGSPMNALEDNAFTRGWQDAAQRIPRPRAIVCVSAHW
                     ETHGVYVGGTPTPETIHDFYGFPKRLFDVRYPAPGDPSLARQVAQTVKNTKVHIDPGR
                     GIDHGAWSVLRIMYPQADIPVLQLSLDSAQPGAFHYALGQELASLRDDGVLILGSGNI
                     VHNLRLFDFHATQPADWAARADALLRAHIESGDHERLLEWNSLGPEVALGIPTPEHYL
                     PLLYVLALRGDGEPMRIFNASVISAISMTCVQVG"
     gene            complement(1761..4088)
                     /locus_tag="VFF05_07545"
     CDS             complement(1761..4088)
                     /locus_tag="VFF05_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZW51697.1"
                     /translation="MSNRSLAPGTAHAATDPVCGMQVDPHSAKHHAEHAGRAYYFCSA
                     RCREKFLAEPVRYLDSTASEVAPPPAGTIYTCPMHPEVRQIGPGHCPRCGMALEPLLP
                     SADSEDDGGLHALSWRFRIAFVLTVPVVALAMGPHLFGWHLPTPWDRIAAWIEAVLAT
                     IVVLWAGAPFFARGWRSLKPWSPNMYTLIALGTGVAWLYSAVAFATPGVFPPAFRDMH
                     GNVGVYFESAAVIVTLVLLGDYLELRARRQTGAALRALIDLAPKIARRIAADGGEDDV
                     PLADVRVGDMLRVRPGEKVPTDGVVLEGTSHVDESMLSGEPMPVEKSAGAKLTGATIN
                     QDGALTMRAEKVGAATLLAQIVALVAQAQRSRAPLQRIADQVAAWFVPAVVAVAVLAF
                     VAWAVFGPDPKLAHALIAAVSTLIIACPCALGLATPISIMVASGRGARAGVLFRDAAA
                     IEALREVDTLVVDKTGTLTEGKPALMGVYTFGGFDESRALALAAALELPSEHPLARAV
                     VAGARERRIAIPKVAEFSTLTGRGVRGAVEGQAVALGNAGLMETAGIVVDAAARARAD
                     VLRGQGATVVFLGVDGALAALIAVADRIKPSSRDAIRQLREAGLRIVMLTGDNATTAW
                     TVAHALLIDEVRAEVSPADKAAALAALKREGRRVAMAGDGINDAPALAAADVGIAMGS
                     GTDIAMESAQVTLVKGELTAIVRARALSQATVRNIHKNLFFAFVYNAIGVPIAAGVLY
                     PWFGIVLSPMFAALAMSLSSVSVVTNALRLRRAKL"
     gene            complement(4132..>5694)
                     /locus_tag="VFF05_07550"
     CDS             complement(4132..>5694)
                     /locus_tag="VFF05_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW51698.1"
                     /translation="VLDAVLRRPGIALLLSAMLLASAALPLARLGSEFLPPMDEGTLL
                     YMPTALPGLSAGKAAQLLQLTDRMIKTVPEVAHVFGKAGRADTATDPAPLEMFETTIT
                     FKPRQQWRPGMTMDKIKVELDRAVKVPGLTNLFVPPIRNRIDMLATGIKSPIGIKVLG
                     ADAVTLENVADRIEVVARGVAGVRSAVAERSAGGRYVDVDIHRNAAARYGLSQRQVQE
                     LIARVVGGEPIGQTIQGRERFPIVLRYARAERDSLSALRALPIVAAGGAQLTLGQIAD
                     IEVQPGPSMLRSEDGRLATYVYVDTAGRDLGAVVADLKRAVAESASLPPGVAVEWSGQ
                     FEYLARATARLTLIVPIVLAIIYLLIWVVFRRADEAALIMLSVPLALVGGLWLIWLLG
                     HAVSVATVIGFIALGGVAAEFGVVMLLYLRHSWDRRRALDVAAGADALDDAIREGAVQ
                     RVRPKAMTVAVVLAGLAPIMAGHGAGAEVMQRIAAPMLGGMLTAPLLSMLVIPAAFAL
                     LERRRLRCSAGP"
BASE COUNT         1004 a         2041 c         1808 g          841 t
ORIGIN      
        1 atccaggctt tcttcgccct ggcgtcttga cactggcgac gacgccgaat tgttgctcat
       61 cgatgtcgat accgtctacg gccacatgga ctggctgcgc gcacacaatt cgcgcctcgt
      121 cgccgtactt accggccaca ctccaggcga cgaatacgag atggtgctgc acaagccgct
      181 gaccgtcgac aatctggtgc aggttctcga tcgcgccgcc gcgctgacca atgaaggccc
      241 tgccgacgcg cccgcgccgc cccccgcccc ggcgccgaaa aaaactgttg cgaaagccgc
      301 gccggcgccc aagcccgtcg cggccgcaac gccagtcgcg gcgcccgcac ccgaaccgcc
      361 gcccgtgccg gaaccgccga aagaacgccg cctgcatcat tggctcgccg atcacgcgct
      421 cgccgccgcc gtgcgcgtgc acagcaagga tgcgcccgat ctggtgctgg atcccggcaa
      481 caagacgtat tacgccgacg gcaatctgcg cgcgctcgcc gcgcattgcg gacgcgtcgt
      541 cgcgcacgag gaattgcacg aggtcagtcc gatcgaactg gcgcgcctgc aagcttcggg
      601 caagggccag ccatatgcgc gcctgaactg gctcagccat gtgctgggct ccaacggcca
      661 tccgacacag ggcgtggacg tgaacgcgaa gttcaagatg tcgcgctggc cgcagatcga
      721 gcgcgagttc ccgaaacatt tccgcatcgc caccgtgatg atgaagcaac cggccacgct
      781 cggcgaaatc gccgaacaaa gcggcgccac cttgccggac gtcatcgatt tcaccaacgc
      841 ctaccacgca atcggtttca tcgaaatcga acgtacggca gcggccgccg aagccgctgc
      901 gccgcgcgat accggtcgcg gtgcgatatt gtcgcgcctc aagaacccgt tcggcagcgg
      961 cacctaggca actcgccttc cccctgtggc gaaccggatg ccagcgttgt tcgtaggcca
     1021 cggctcgcca atgaacgcgc tggaagacaa tgccttcacg cgcggctggc aggatgcggc
     1081 gcaacgcatt ccgcggccgc gcgcgatcgt ttgcgtatcc gcgcattggg aaacgcacgg
     1141 cgtatacgtt ggcggcacgc cgacgccgga gaccatccac gatttctacg gcttccccaa
     1201 gcgtttgttc gacgtgcgct acccggcgcc cggcgatccg tccctggcgc ggcaggtcgc
     1261 acagacggta aaaaacacaa aagtacacat cgaccccggc cgcggcatcg accacggcgc
     1321 gtggagcgtg ctgcgcatca tgtatccgca ggcggacatc cccgtgctgc aactgagcct
     1381 ggacagcgcg cagcccggcg cattccacta cgcgctcggc caggaactcg cgtcgctgcg
     1441 cgacgacggc gtacttattc tgggcagcgg caacatcgtg cacaacctgc gcctgtttga
     1501 cttccacgcc acgcagccgg cggactgggc cgcacgcgcc gacgcgctgc tgcgcgccca
     1561 catcgaatcc ggcgatcacg aacgcctgct ggagtggaat tcacttggcc cggaagtcgc
     1621 gctcggcatc cccacgcccg agcactacct gccgctgttg tacgtcctcg ccttgcgtgg
     1681 cgacggcgag ccgatgcgca tcttcaacgc cagcgtcatc agcgcgatct ccatgacctg
     1741 cgtgcaggtc ggttgagcgc tcataatttc gcgcggcgca atcgcaacgc gttcgtcacc
     1801 accgaaaccg acgacaggct catcgccagc gccgcaaaca tcggcgacag cacgatgccg
     1861 aaccacggat acagcacgcc cgccgcgatc ggcacgccga tggcgttgta gacgaaggcg
     1921 aaaaagaggt tcttgtggat gttgcgcacg gtcgcctgcg acaacgcgcg cgcgcgcacg
     1981 atcgcggtca gttcgccttt caccagcgtg acctgcgcgc tttccatggc gatgtccgtg
     2041 ccgctgccca tggcgatgcc gacatcggcg gcggcgagcg ccggcgcgtc attgatgccg
     2101 tcgccggcca tcgccacgcg tcggccttcg cgcttgagcg ccgccagcgc cgccgccttg
     2161 tccgcgggcg acacctcggc gcgcacctcg tcgatcagca gcgcatgcgc caccgtccag
     2221 gcggtcgtcg cgttgtcgcc ggtgagcatg acgatgcgca ggcccgcctc gcgcagttgc
     2281 cggatcgcgt cgcgtgaact cggcttgatg cgatcggcca cggcgatcag cgcagctagc
     2341 gcgccatcga cgccgaggaa caccacggtg gcgccctggc cgcgcagcac atcggcgcgc
     2401 gcgcgcgcgg cggcgtcgac gacgatcccg gccgtctcca tgaggcccgc gttgcccaac
     2461 gcgacggcct gcccttcaac tgcgccgcgc acgccgcgcc cggtcagcgt ggagaattcc
     2521 gcgaccttcg ggatggcgat gcgacgctcg cgcgcgcccg ccacgaccgc acgcgcgagc
     2581 ggatgctcgc tcggcaactc cagcgcggcg gccagcgcca gggcgcgcga ctcgtcgaag
     2641 ccgccgaacg tgtatacgcc catcaatgcc ggcttgcctt cggtcaaggt gccggtcttg
     2701 tccacgacca gcgtgtcgac ctcgcgcagc gcctcgatgg cggcagcgtc gcgaaacagc
     2761 acgccggcgc gcgcgccacg tccactcgcg accatgatcg aaatcggcgt ggcgaggccc
     2821 agcgcacacg gacaagcaat gatcagcgtc gagaccgcgg cgatcagggc atgcgccagt
     2881 ttcggatccg gaccgaacac cgcccaggcg acgaaagcga gcacggccac cgcgacgact
     2941 gccggcacga accacgccgc gacctggtcc gcaatgcgct gcagcggcgc ccgggagcgc
     3001 tgcgcctgcg caaccagcgc aacgatctgc gccagcaggg ttgcggcacc caccttttcg
     3061 gcacgcatgg tcaaggcgcc gtcctggttg atcgtggctc cggtcagttt ggcgccagcg
     3121 gatttttcga ccggcatcgg ttcgccactg agcatggact cgtcgacatg actcgtacct
     3181 tcaagcacga cgccatcggt cggcaccttc tcgcccgggc gtacgcgcag catgtcgccg
     3241 acacgcacgt cggccagcgg cacatcgtcc tcaccaccgt ccgctgcgat gcggcgcgca
     3301 atcttcggcg ccagatcgat gagagcgcgc aaggcagcgc cggtttgccg gcgcgcgcgc
     3361 agttcgaggt aatccccgag cagcaccagc gtcacgatga ccgcagccga ttcgaaatac
     3421 accccgacgt tgccgtgcat atcgcgaaat gcgggtggaa acacgccggg cgtggcaaac
     3481 gccaccgcgc tgtacaacca ggccacaccc gtaccaagcg ctataagcgt gtacatgttc
     3541 ggcgaccagg gtttgagcga gcgccagcca cgcgcgaaga atggcgctcc ggcccacagc
     3601 acgacgattg tcgccaacac cgcctcgatc cacgccgcga tgcgatccca cggtgtcggc
     3661 aggtgccagc cgaacaggtg cgggcccatc gccagcgcga cgaccggcac ggtcagcacg
     3721 aatgcgatcc gaaagcgcca agacagtgcg tgcaatccgc catcgtcctc ggaatccgcg
     3781 ctcggcagca aaggctccag cgccatgccg cacctgggac aatggcccgg gccgatctgc
     3841 cgcacttccg ggtgcatcgg gcaggtgtag atcgttccgg ccggtggcgg cgcgacttcg
     3901 ctggcagtgc tgtcgagata ccgcaccggc tcggcgagga atttctcgcg gcaacgcgcg
     3961 gaacagaaat agtacgcgcg gcccgcatgt tcggcatggt gtttggccga gtgtggatcg
     4021 acctgcatgc cgcagaccgg atcggtcgcg gcgtgcgcgg tacccggcgc gaggctgcgg
     4081 ttgctcatgt gcaattccca ttcgtgcaat gcatacagga tacgtccgcc gtcaaggccc
     4141 ggcactgcac cgcaaccggc gccgctccag caacgcaaag gccgccggta tgaccagcat
     4201 cgacaacagc ggcgcggtca gcatgccgcc gagcatcggc gcggcaatgc gttgcatcac
     4261 ttccgcaccc gcgccgtggc cggccatgat cggcgccagg ccggccagca ccacggccac
     4321 ggtcatcgcc tttggccgca cgcgctgcac ggcgccttcg cggatcgcat cgtcgagcgc
     4381 atccgcgccg gcggcgacat cgagtgcgcg ccgccgatcc caggaatgac gcaggtacag
     4441 cagcatcacc acgccgaact cggcggcgac gccgccaagc gcgatgaagc cgatgaccgt
     4501 cgccaccgat accgcatggc cgagcagcca gatcagccac aacccgccga ccagagccag
     4561 cggcacgctg agcatgatca gcgccgcttc gtcggcgcgg cggaacacca cccagatcag
     4621 caaatagatg atcgcgagca cgatcggcac gatcagcgtc agtcgtgcgg ttgcccgcgc
     4681 caggtactcg aattgcccgg accattcgac ggcgacgccc ggtggcagcg acgcggattc
     4741 ggcgacggcg cgtttcaggt ccgcgaccac cgcgccgagg tcgcgccccg ccgtgtcgac
     4801 gtagacgtag gttgccagac ggccgtcttc gctgcgcaac atcgatggcc ctggctgcac
     4861 ctcgatgtcg gcgatctgcc cgagcgtgag ctgcgcgccg ccggcggcga cgatcggcaa
     4921 tgcgcgcagt gcggacagcg aatcgcgttc cgcccgcgca tagcgcagca cgatcggaaa
     4981 gcgctcgcgg ccctggatgg tctgcccgat cggctcgccg ccgaccactc tggcgatcag
     5041 ctcctgtacc tggcgctggc tcaatccgta gcgcgccgcg gcattgcgat ggatgtcgac
     5101 atcgacgtag cggccgccgg cgctgcgctc ggcaaccgcc gagcgcacgc ccgccacgcc
     5161 gcgcgcgacg acttcgatgc gatcggcgac gttttccagc gtgaccgcgt ccgcaccgag
     5221 caccttgata ccgatcggac tcttgatgcc ggtcgcgagc atgtcgatgc ggttgcgaat
     5281 cggcggcacg aacaggttgg tcagtcccgg caccttcacc gcgcggtcga gttcgacctt
     5341 gatcttgtcc atggtcatgc ccggccgcca ctgctgcctg ggcttgaatg tgattgtggt
     5401 ctcgaacatt tccagtggtg cgggatccgt ggcggtgtcg gcacggccgg ccttgccgaa
     5461 cacgtgggcg acttccggca ccgtcttgat catgcggtcg gtgagctgca gcagttgcgc
     5521 ggccttgccg gcggacagcc cgggcaaggc cgtgggcatg tacaacaacg tgccctcgtc
     5581 catcggcggc aggaattcgc tgcccagccg cgccagcggc agcgccgcgc tcgccagcag
     5641 catggcggac aaaagcaggg cgatgccggg acggcgcaac accgcatcga gcac
//
LOCUS       DASRVO010000612         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_18122, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000612 DASRVO010000000
VERSION     DASRVO010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18122"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VFF05_07555"
     CDS             <1..1673
                     /locus_tag="VFF05_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZW51699.1"
                     /translation="ELHEALMGLGFLTAADMARNPTWSTLFDALIALRRATRLANLCI
                     PAERLPQFQTIFAGAAFDPPISAPAEFAQVEWTRETALADLLRARLTGLGPTQAAQLA
                     EMLNLPQVEIDAALLALEREGTAMQGHFTPDPATSDVEWCERYLLARIHRYTIHRLRR
                     EIEPVEPRDFMRFLFDWQKVSSGTRVEGPDALAAVLAQLEGFEAPAAAWESEILPARV
                     AGYEISWLDDLCLAGRIVWSRLRPRTSTGSEHGTGPVRATPIVLLQRRSLSIWNALDA
                     TKDDGDAAISSRATKVSDFLRSHGASFFDELLAGTQLLHVELEEALAELVAAGRINSD
                     SFAGLRALLVPQSKRPSPSRRRGRRTALLGIADAGRWSPLRRGSAPGDSSNPQNANVE
                     NVEYIARTLLRRYGVVCWRLLAREAAWLPPWRELMRAYQRMEARGEVRGGRFIAGLSG
                     EQFALPEAVGLLRGIRRKPGDGEMVAVCGADPLNLVGAIVAGEKVPALTGSRILYRDG
                     TPVATLVSGNFAALEPLDAALEWKAKGFLLRGGECEPTAAQTEAQRPQ"
     gene            complement(1658..1981)
                     /locus_tag="VFF05_07560"
     CDS             complement(1658..1981)
                     /locus_tag="VFF05_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HZW51700.1"
                     /translation="MFMRKLALAAALGVVGFGAAYAPPASAGAAVGVSVRLPVHAPPP
                     PIVERVGVRPGYAWIPGYWRWGGARYVWVGGYWTHPRRGYRWVEPRWVGCGPHWCMHR
                     GYWGR"
BASE COUNT          370 a          800 c          739 g          372 t
ORIGIN      
        1 acgaattgca cgaggcgttg atgggacttg gcttcctgac cgcagccgac atggcgcgca
       61 atccgacgtg gtccacgctg ttcgatgcgc tgatcgcgct gcggcgggcg acgcgcctgg
      121 cgaacttgtg catccctgcc gaacgcctgc cgcaattcca aacgattttc gctggcgctg
      181 cgttcgatcc gccaatttcc gcgccggcgg aattcgcgca agtcgaatgg acgcgcgaaa
      241 ccgcgctcgc cgaccttctg cgcgcgcgcc tgaccggact cggtccgacg caggccgcgc
      301 agctcgccga gatgctgaat cttccacaag tggaaatcga tgccgccctg ctggcgctgg
      361 agcgcgaagg cacggcgatg cagggccatt tcacgcccga tcccgcgact tcagacgtgg
      421 aatggtgtga acggtacctg ttggcgcgca tccaccgtta cacgatccat cgcctgcgcc
      481 gcgagatcga gccggtcgag ccgcgcgact tcatgcgctt tctgttcgac tggcagaaag
      541 tgtcttccgg cacccgcgtc gaaggcccgg atgcactcgc cgccgtgctc gcgcaactgg
      601 aaggcttcga ggcgccggcg gcggcctggg aatcggaaat cttgcccgcg cgcgtcgccg
      661 gctacgagat ttcctggctc gacgaccttt gcctcgctgg gcgtatcgtg tggtcgcggc
      721 tgcggccacg cacatccact ggcagtgaac acggaaccgg ccccgtgcgc gccacgccca
      781 tcgtcctgct gcaacgacgc agcctgtcga tttggaatgc attggatgcg acgaaagacg
      841 atggcgatgc cgcgatctcg tcgcgtgcaa cgaaggtttc cgatttcctg cgcagccacg
      901 gcgcgtcgtt cttcgatgaa ctgctggcgg gcacgcaact gctgcatgtc gaactggaag
      961 aagcgctggc cgaactcgtt gccgcgggac ggatcaattc cgacagcttc gccggcctgc
     1021 gcgcgctgct ggtgccccaa tccaaacgcc cctcgccgag ccggcgtcgc ggccgccgca
     1081 cggccctgct cggcatcgcg gatgccggtc gctggtcgcc gctgcggcgc ggctccgcgc
     1141 cgggcgattc cagtaatccg caaaatgcca atgtggaaaa tgtcgagtac atcgcccgta
     1201 ccctgctgcg ccgctacggg gtggtctgct ggcgcctgct cgcgcgcgag gcggcgtggc
     1261 tgccgccgtg gcgcgagctg atgcgcgcgt accagcgcat ggaggcgcgc ggcgaagtcc
     1321 gcggcggccg tttcatcgcc gggctctccg gcgaacagtt cgcgctgccc gaggcggtcg
     1381 ggctcctgcg cggcatccgc cgcaagccgg gcgacggcga gatggtcgcg gtgtgcggcg
     1441 cggatccgct gaacctggtc ggcgccatcg tcgccggcga gaaagtgccg gcactgaccg
     1501 gatcgcgcat cctctatcgc gatggaacgc ccgtcgcgac cctggtttcc ggtaatttcg
     1561 ccgcgctcga accgttggat gcggcgctgg aatggaaggc caagggcttc ctgttgcgcg
     1621 gcggcgagtg cgaacccacc gccgcgcaga ccgaagctca acgcccccag tagccgcggt
     1681 gcatgcacca atgcgggcca cagccgaccc agcgcggctc aacccagcgg tatccgcggc
     1741 gcggatgcgt ccagtaaccg ccgacccaga catagcgcgc gccgccccag cgccaatagc
     1801 ccggaatcca ggcgtaaccc ggtcgcacgc cgacgcgctc gacgattggc ggcggcggcg
     1861 catgcacggg caagcgcacc gaaacgccca cagcggcacc ggccgaagcc ggcggcgcat
     1921 atgcagcccc gaatccgacg acacccaggg ccgctgccaa agccagtttg cgcatgaaca
     1981 tgatcgttct ccgtttgcct ccagccgcat gattgcggcg gttaccctga aaaacgccgc
     2041 agactgcgcc gtgttgacgt ccagcgctgc ggcgttcagc cggcgttttt cgatcacggc
     2101 ttgccgacgc ggcgaaaacc cggcatcgtg cgcgttttca aacgcacgtt cgacgccatg
     2161 accgacgcgg ccgccacgcc ctatccgcac ctgttcgctc cgctcgatct cggcttctgc
     2221 acgttgccca accgcgtgct catgggctcg atgcacactg ggctcgaaga ccatgcacgc
     2281 g
//
LOCUS       DASRVO010000613         1202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_18142, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000613 DASRVO010000000
VERSION     DASRVO010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18142"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFF05_07565"
     CDS             <1..258
                     /locus_tag="VFF05_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HZW51701.1"
                     /translation="GELLAPPDADEACAILCGHLEALYAFYGEIAGVRIARKHLSWYA
                     KDRPENSAFRAIVNRAESAHEQLRLTREYFAALAADWPIAA"
     gene            378..>1202
                     /gene="metK"
                     /locus_tag="VFF05_07570"
     CDS             378..>1202
                     /gene="metK"
                     /locus_tag="VFF05_07570"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZW51702.1"
                     /translation="MSNYLFTSESVSEGHPDKVADQISDAVLDAILAQDKRARVACET
                     MVKTGVAIVAGEITTSAWIDLEALTRKVILDIGYDSSDVGFDGATCGVLNLIGKQSPD
                     INQGVDRRKPEEQGAGDQGLMFGYACTEAPEFMPAPIYYSHRLVEQQAKVRKAGKLPW
                     LRPDAKSQVTLRYDSAHKVTGLDAVVLSTQHDPQIKHADLVDGVRELILKPVLPKKWL
                     DALAKNKVHINPTGKFVIGGPVGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVD"
BASE COUNT          228 a          413 c          378 g          183 t
ORIGIN      
        1 ggcgagctgc tcgcgccgcc ggacgcggac gaggcgtgtg cgattctgtg cggccatctc
       61 gaagcgctgt acgcgttcta cggcgaaatc gccggcgtgc ggatcgcgcg caagcacctc
      121 tcctggtacg ccaaagatcg tccagagaac tcggcattcc gggccatcgt caatcgcgcc
      181 gaaagcgcgc acgagcaact gcgcctgacg cgcgagtatt tcgccgcact ggcagcggac
      241 tggccgatcg cagcctagcc gtttagacgt ccgaacatga atcgcggcac aacaccggct
      301 tgacgcggct gcaaagccgc gccgcccgtt gtatcatgcg cggctttccc cgttcccttg
      361 tgcggatatc cagcccgatg agcaattacc tgtttacctc cgaatccgtg tccgaaggcc
      421 atccggacaa ggtcgccgac cagatttccg acgccgtgct cgacgcgatc ctcgcgcagg
      481 acaagcgcgc gcgcgtggcc tgcgaaacca tggtcaagac cggcgtcgcc atcgtcgccg
      541 gcgaaatcac gaccagcgcc tggatcgacc tcgaggccct gacgcgcaag gtcatcctcg
      601 atatcggcta cgactcgtcg gacgtcggct tcgacggcgc tacctgcggc gtgctgaacc
      661 tgatcggcaa gcagtcgccg gacatcaacc agggcgtgga tcgcaggaaa cccgaggaac
      721 agggcgccgg cgaccagggc ctgatgttcg gctacgcctg caccgaggcg ccggaattca
      781 tgcccgcgcc gatctactac tcgcaccgcc tcgtcgaaca gcaggcgaag gtgcgcaagg
      841 ccggcaagct gccgtggctg cgtccggacg cgaagtcgca ggtcacgctg cgctacgaca
      901 gtgcgcacaa ggtgaccggc ctcgacgccg tggtcctgtc cacccagcac gatccgcaga
      961 tcaagcatgc cgacctcgtc gacggcgtgc gtgaactcat cctcaagccc gtgctgccga
     1021 agaagtggct ggacgcgctg gcgaagaaca aggtgcacat caacccgacc ggcaaattcg
     1081 tgatcggcgg tccggtcggc gactgcggcc tgaccggacg caagatcatc gtcgacacct
     1141 acggcggcat ggcccgccac ggcggcggcg cgttctcggg caaggacccg tccaaggtcg
     1201 ac
//
LOCUS       DASRVO010000614         5936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_355062, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000614 DASRVO010000000
VERSION     DASRVO010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355062"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFF05_07575"
     CDS             <1..385
                     /locus_tag="VFF05_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZW51703.1"
                     /translation="SLDSLRDTGSAKAWLITILRREHARLYERKTPQFADVDEIEVAD
                     ERETHTPESVGEDGLIRAAMQRLDAKYREPLLMQVLGGFSCEEIARELNITPAAVMTQ
                     VFRARQKLKGMLAGDVKEAQLHELS"
     gene            372..1079
                     /locus_tag="VFF05_07580"
     CDS             372..1079
                     /locus_tag="VFF05_07580"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HZW51704.1"
                     /translation="MNCLEFRRELNIHPHSSAAEFVAHARECPRCAQAQADALAFEST
                     LGRALAIPPPPNLAESILLAQATQQRRERQRWASGAGWLTLAAAAVLAVGVGWRVTRA
                     QPLGDAAIAHMLGAEAPALAMTAPVAAGDVRKAFAKRGVDLTQVPADISYVQCCPVGK
                     FKSVHMVMPRANGPVTVMYLVDDKGSTRADFAHAGWIGRSVPMAHGTLVMIGHDASQF
                     AQIEQEWQSTLQASTRI"
     gene            complement(1134..2969)
                     /locus_tag="VFF05_07585"
     CDS             complement(1134..2969)
                     /locus_tag="VFF05_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51705.1"
                     /translation="MSGRHSFLAELQRRNVPRAALLYIGAVWALAQGISQLSPALGLS
                     GWTTRWFLIAAIIGFPFWIAFAWVYEWTPQGFKRESEIAQDASIMRSTGRKLDKAIIA
                     VLAFAVVLLLANTFVWKKGAGLHDGSDTGIPARSIAILPFEDLSPAHDHGYFSAGLAE
                     ELLNALAKVRDLKVAGRASSFYYGNHNADLRTIGKALDVANVLEGSVRSQGDTVRISV
                     QLVRSVDGFEVWSQSYDGDLHNIFDVQERIAHAVTDHLKVVLTGSEQTRLVPVTTTSA
                     EAYADFVAAQTLVNQRVGDSLPRAIALLDKATQLDPKFARAWSKLAVAYAVLPQYTQA
                     DWNASWQSSDADARRALALDADDAEAYAALSYNQFSQRHYVAMVEPMQRAVELAPDNS
                     AVNYWAANELAAMGRTRDAEARIDAVLVNDSANTLLLFYKSMIRWRQGDQAGALAYIH
                     RGGVTDSPFGALMLEFYDAAHGDMAGSARDFANSTSKMGTKISLTDLQAIYRGTYGDA
                     AHHQTALKILETHLDDDWAPTLLLQLGEPAPSFHLYEHGHSGLSDGYLNWLWQPEPWS
                     RKARQDPAFQGFAKRIGMLDYWKRYGWPEFCQPAPAKGPDAFDCR"
     gene            3095..4069
                     /locus_tag="VFF05_07590"
     CDS             3095..4069
                     /locus_tag="VFF05_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZW51706.1"
                     /translation="MTILVTGAAGFIGAYVARALLARGERVVGLDNFNDYYDPQLKRD
                     RVAALCPTLDLRTLDLADKAALDALFAEIKPDRVIHLAAQAGVRYSLKNPYAYLHSNL
                     AGFLNMLEACRHGSVRHLVYASSSSVYGRNAVAPFAETQAVDQPLSLYAATKASNELL
                     AHCYAHLYRLRCTGLRFFTVYGPWGRPDMAPLLFSRAILAGRPIEVFNHGKMRRDFTH
                     IDDIVAGVLGALGHAQADDGVNPPCAVFNLGNHRPVELEHFIAVIEKAAGRRAQRIDK
                     PMQPGDMVETSAAVARARAAFGFEPRITIDEGMPAVVGWCREYFGDKL"
     gene            4116..4832
                     /locus_tag="VFF05_07595"
     CDS             4116..4832
                     /locus_tag="VFF05_07595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW51707.1"
                     /translation="MPQLSVVVPVFNERGNIVPLLTEIATALRGKVDLEVVYVDDASR
                     DDSLAVLAQAKAQFPELRVLRHLAQSGQSTAIRTGIRAARGAWIATLDGDGQNDPADI
                     PKLIAARDAGAMDVKLYAGWRVNRKDSGSKRWASKFANAIRSRLLRDATPDTGCGIKL
                     IERAAYLDLPYFDHMHRYLPALMQRAGWKTVSVPVNHRPRGTGQSKYNNLNRALVGIA
                     DLRGVAWLIKRSKKTGVEEI"
     gene            4838..5587
                     /locus_tag="VFF05_07600"
     CDS             4838..5587
                     /locus_tag="VFF05_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZW51708.1"
                     /translation="MAASALLQLQGVSRRLAGRTIVEDLTLSLDRGCVLGLLGVNGAG
                     KSTTLRMIAGVLAPSAGKVLFDGRDLGEEPALARRLGYLPETPPLYAELRVDEYLAFC
                     ARLRGMRSAQAKAAVDRVIKRCGLGEVRRRLCANLSKGFAQRAGIAQAIVHNPDLIVL
                     DEPASGLDPVQAASIRKLVGELGRERAVILSTHLLHDVTACCARVAILHCGRLRHDGT
                     LADLCGNDAEGGSLEKVFFRIASADDAQAAA"
     gene            5584..>5936
                     /locus_tag="VFF05_07605"
     CDS             5584..>5936
                     /locus_tag="VFF05_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZW51709.1"
                     /translation="MSRVVARNEWRRLFAQPLAWVLLAATLAVLAYFFLLTLQGFLGL
                     SPKLAGMPSPPGVTDLVALPLMRAIGSVLLLVAPLLGMRAIAGERQSGTLPLLQSSGT
                     GGAGIVLGKFAALCG"
BASE COUNT         1008 a         1990 c         1908 g         1030 t
ORIGIN      
        1 ttcgctggac agcctgcgcg acacgggatc ggccaaggcc tggctgatca cgattctgcg
       61 ccgcgaacat gcgcgtctgt acgagcgcaa gacgccgcag tttgccgacg tggacgagat
      121 cgaggtggcc gatgagcgcg agacgcatac gccggaatcg gtcggcgagg acggcctgat
      181 ccgcgccgcg atgcaacggc tggatgccaa gtaccgcgag ccgctgctga tgcaggtgct
      241 cggcggattt tcctgcgagg agatcgcgcg ggaactgaac atcacgccgg ccgcggtgat
      301 gacgcaagtt ttccgcgcgc ggcaaaagtt gaaaggcatg ctcgcgggcg atgtcaaaga
      361 agcgcagtta catgaattgt cttgaattcc gccgtgaact gaacatccat ccgcattcga
      421 gcgctgcgga attcgtcgcg catgcgcgcg aatgtccgcg ctgcgcgcag gcgcaggcgg
      481 atgcgctcgc gttcgaatcc acgctcggcc gcgcgctggc gattccgccg ccgccgaacc
      541 tggccgagtc catcctgctc gcgcaggcca cgcaacagcg ccgcgaacgc cagcgctggg
      601 ccagtggggc gggctggctc acgctcgccg cggccgcggt gcttgccgtg ggcgttggct
      661 ggcgcgtcac gcgcgcgcag ccgctggggg atgcggcgat cgcgcacatg ctcggcgcgg
      721 aagcgccggc gctggccatg accgcaccgg tcgctgccgg cgacgtgcgc aaggcgttcg
      781 ccaagcgcgg cgttgacctg acccaggtgc ccgccgacat cagctacgtg cagtgctgcc
      841 cggtcggcaa gttcaagtcc gtgcacatgg tgatgccgcg cgcgaacggc ccggtcacgg
      901 tgatgtacct ggtcgacgac aagggcagta cgcgtgccga tttcgcgcat gccggctgga
      961 ttggccgcag cgtgccgatg gcgcacggaa cattggtgat gatcggccac gatgccagcc
     1021 agttcgcaca gatcgagcag gaatggcaat cgacgctgca agcctcgacg cgcatctgag
     1081 tccatcccgg ttccaggtcg ttcgcggttt gaacgccaac cgctagccag cgctcatcgg
     1141 caatcgaaag cgtccggccc cttggcggga gcgggttggc aaaactcggg ccagccgtag
     1201 cgtttccagt aatcgagcat gccgatgcgt ttggcgaatc cctggaacgc cgggtcttgc
     1261 cgggccttgc gcgaccaagg ctctggttgc cacagccagt tgaggtatcc atcggacaag
     1321 ccgctgtgac catgctcgta caaatgaaat gaaggcgcag gctcgcccag ttgcaggagc
     1381 aacgtcggtg cccagtcatc gtccaaatgc gtctccagga ttttcaaggc agtttggtgg
     1441 tgtgcagcat cgccataggt gccgcgataa atggcctgca aatccgtgag agaaatcttc
     1501 gtgcccattt tgctggtcga gttggcgaaa tcccgggcgg atcccgccat gtcgccgtgc
     1561 gcggcgtcat agaactccag catcagcgca ccgaacggcg aatccgtcac gccgccgcgg
     1621 tggatgtacg ccagtgcgcc cgcctgatcg ccctggcgcc aacggatcat gctcttgtag
     1681 aacagcagca acgtgttcgc cgagtcgttc accagtaccg catcaatccg tgcttccgca
     1741 tcgcgcgtgc ggcccatggc cgcaagttcg ttcgcggccc agtaattcac ggcggaattg
     1801 tccggcgcaa gttccaccgc gcgttgcatc ggctccacca tcgccacgta atgccgttgt
     1861 gagaactggt tgtagctgag cgccgcataa gcctccgcgt catcggcgtc cagtgcaagc
     1921 gctcgccgcg cgtcagcgtc ggatgattgc caactggcat tccagtcggc ttgcgtgtac
     1981 tgcggaagca cggcataggc gaccgcgagt ttcgaccagg cgcgcgcaaa tttcggatcg
     2041 agctgggttg ccttgtccag cagcgcgatc gcgcgcggca ggctgtcgcc cacgcgctgg
     2101 ttgaccagcg tctgcgcggc gacgaaatcc gcgtaggctt ccgcgctggt ggtggtgacc
     2161 ggtaccagcc gcgtctgctc gctgccggtc aggacgacct tcaggtgatc ggtgaccgca
     2221 tgcgcgatgc gctcctgcac atcgaaaata ttgtgcagat cgccgtcgta gctctgcgac
     2281 cacacctcga aaccgtcaac ggagcggacc agttgcacgc tgattcgcac ggtgtcgccc
     2341 tgcgagcgca ccgaaccttc cagcacgttg gcgacatcga gcgccttgcc gatggtgcgc
     2401 aggtcggcgt tgtgattgcc gtagtagaac gacgaggcgc gcccggcgac cttcaaatcc
     2461 cgcaccttgg ccagcgcgtt caacagctct tcggcgaggc cagccgagaa ataaccgtgg
     2521 tcgtgcgccg ggctgagatc ctcgaacggc agtatcgcaa tggacctggc cggaatcccg
     2581 gtgtccgatc catcatgcaa gccggcgccc tttttccaga caaaggtgtt ggcaagtaaa
     2641 agcaccacgg cgaacgccag cacggcaatg attgccttgt cgagtttgcg accagtcgag
     2701 cgcatgatcg acgcgtcttg cgcaatttcg ctttcacgct tgaagccctg cggggtccac
     2761 tcgtaaaccc acgcgaatgc gatccagaac ggaaagccga tgatcgcggc tatcagaaac
     2821 cagcgcgtag tccagccaga caaaccgagg gcggggctca actgcgagat gccctgcgcc
     2881 agcgcccata ccgcgccgat gtagagcaac gctgcgcgcg gcacattgcg gcgctggagt
     2941 tccgccagga aactgtgacg acccgacacg gcgcggtgcg ctccccgccg gcgacggccg
     3001 gcctcggcga cgcgaagttt gcggcttttg tggcgatgcg cgcaagccgc taaaatcacg
     3061 tcttcgcgcc ggtgccggcg ccgcaggact tcgcatgacc attcttgtta ccggcgcggc
     3121 cggcttcatc ggcgcttatg tcgcccgcgc cctgctcgcg cgcggcgaac gcgtcgtcgg
     3181 cctcgacaat ttcaacgact actacgatcc gcaactcaag cgcgatcgcg tcgccgcgct
     3241 gtgcccgacc ctcgacctgc gtacgctcga tctcgccgac aaggccgcgc tcgacgcgct
     3301 gttcgccgaa atcaaaccgg atcgcgtgat ccatctggcc gcgcaggccg gcgtgcgcta
     3361 ttcgctgaag aatccgtacg cctacctgca cagcaacctg gccggcttcc tgaacatgct
     3421 cgaagcctgt cgccacggca gcgtgcgaca cctggtctac gcctcgtcgt cttcggtgta
     3481 cggacgcaac gccgtcgcgc cattcgccga gacgcaggcc gtcgatcagc cgctgtcgct
     3541 gtacgcggcg accaaggcct cgaatgaact gttggcgcat tgctacgcgc acctgtaccg
     3601 tctgcgctgc accggactgc gtttcttcac cgtgtacggc ccctggggcc ggccggacat
     3661 ggcgccgctg ttgttctcgc gtgctatcct cgctgggcgg ccgatcgagg tgttcaacca
     3721 tggaaaaatg cgccgcgact tcacccacat cgacgacatc gtcgccggcg tgctcggcgc
     3781 actcgggcat gcgcaagcgg acgatggcgt gaatccgccc tgcgcggtct tcaacctcgg
     3841 caaccaccgt ccggtcgagc tcgagcactt cattgccgtg atcgagaagg ccgccggccg
     3901 ccgtgcgcag cgcatcgaca agccgatgca gcctggcgac atggttgaga cgagtgccgc
     3961 cgtggcacgt gcccgcgcgg cattcggatt cgagccgcgc atcacgatcg acgagggcat
     4021 gcccgccgtt gtcggctggt gccgcgagta ttttggcgac aagctctagc gcgacacggc
     4081 ataatcgcgc cacgcgtttt ccggaaaatc ccgtgatgcc ccaactgtcc gtcgtcgtgc
     4141 cggtgttcaa cgagcgcggc aatatcgttc cgctgctcac cgagatcgcg actgcattgc
     4201 gcggcaaggt cgatctcgag gttgtctacg tcgacgacgc gagccgcgac gactcgctcg
     4261 ccgtgctcgc ccaggccaag gcgcagtttc cggaactgcg cgtgctgcgg cacctggcgc
     4321 agagcgggca gagcaccgcg atccgcaccg gcatccgggc tgcacgcggc gcgtggatcg
     4381 ccacgctcga cggcgatggc cagaacgatc cggcggacat cccgaaactg atcgccgcgc
     4441 gcgatgcggg tgcaatggac gtgaagctct acgccggctg gcgcgtgaac cgcaaggatt
     4501 ccggcagcaa gcgctgggcc tcgaaattcg ccaacgcgat ccgctcgcgc ctgctgcgcg
     4561 acgccacccc cgacaccggc tgcggcatca agctgatcga acgcgccgcc tacctcgacc
     4621 tgccctactt cgaccacatg caccgttacc tgcccgcact catgcagcgt gccggctgga
     4681 aaaccgtgag cgtgccggtc aaccatcgcc cgcgcggcac cgggcaatcc aaatacaaca
     4741 acctcaaccg cgccctcgtc ggcattgccg atttgcgtgg cgtggcgtgg ctgatcaagc
     4801 gcagcaagaa aaccggcgtc gaggaaatct gacgcgcatg gccgcatccg cgttgctgca
     4861 actgcaaggc gtgagccgcc gcctggccgg gcgcaccatc gtcgaggacc tgacactctc
     4921 gctcgatcgc ggctgcgtgc tcggcctgct cggcgtcaac ggcgccggca agtccaccac
     4981 cttgcgcatg atcgccggcg tgctcgcgcc gagcgcgggc aaagtgctgt tcgacggacg
     5041 cgatcttggc gaagaaccgg cgcttgcgcg gcgcctgggt tatctgccgg aaacgccgcc
     5101 gctgtacgcc gaattgcgcg tggacgaata cctcgcgttc tgcgcgcgcc tgcgcgggat
     5161 gcgtagcgcg caggcgaagg cggccgtgga tcgcgtgatt aaacgctgcg ggttgggcga
     5221 agtgcgccgg cgcctgtgcg ccaatctgtc caagggtttt gcgcagcgcg ccggcattgc
     5281 ccaggccatc gtccacaatc cggacctgat cgtgctcgac gagccggcct cgggcctcga
     5341 ccccgtgcag gcggcgtcga tccgcaaact ggtgggcgaa ctcggtcgcg agcgtgcggt
     5401 gatcctgtcc acgcatctgc tgcacgacgt cactgcctgt tgcgcgcgcg tcgcgatcct
     5461 gcattgtgga cgtctgcgcc acgatggcac actcgccgat ctctgcggca atgatgcgga
     5521 aggcggatcg ctggaaaagg ttttctttcg catcgccagc gccgacgacg cgcaggccgc
     5581 ggcatgagcc gcgtcgttgc ccgcaacgaa tggcggcgcc tgttcgcgca gccgctggcg
     5641 tgggtgctgc tcgccgccac gctcgcggtg ctcgcgtatt tcttcctgct gaccttgcag
     5701 ggctttctcg gcctcagccc caaactcgcc ggcatgccgt caccgccggg cgtcaccgac
     5761 ctcgtcgcct tgccgctgat gcgcgcgatc ggcagcgtgc tgctccttgt cgcgccattg
     5821 ctcggcatgc gcgccatcgc gggcgagcgc cagtccggca ccctgccgct gttgcagtcg
     5881 tcgggcacgg gcggggccgg catcgtgctc ggcaaatttg ccgcgctgtg cggatt
//
LOCUS       DASRVO010000615         1383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_330053, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000615 DASRVO010000000
VERSION     DASRVO010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330053"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFF05_07610"
     CDS             complement(<1..890)
                     /locus_tag="VFF05_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW51710.1"
                     /translation="MPLLQLQRVDYSVGGPLLLEQVDLTIEPNERVCVVGRNGEGKST
                     LLRLLAGEIKPDDGEVRVQGGVRVAKLAQEVPQDARGSVFDVVAQGLGDLGALLARYH
                     HLIAEHGDLTALGAVQAQIEAQHGWDMDRRVSQVLERLELPADADFAALSGGMKRRVL
                     LAQALVRAPDVLLLDEPTNHLDVAAIQWLEDFLRAAPFSLVFVTHDRSFLRALATRIV
                     EIDRGVVTAWPGDYENYLRRREERLHAQAQANALFDKKLAQEEVWIRQGIKARRTRNE
                     GRVRALQAMRREREQRREVSG"
     gene            complement(964..>1383)
                     /locus_tag="VFF05_07615"
     CDS             complement(964..>1383)
                     /locus_tag="VFF05_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HZW51711.1"
                     /translation="FSGKDPSKVDRSAAYAARYVAKNIVAAGIADRCEIQVSYAIGVA
                     QPTSISVTTFGTGKISDDKIEKLIRKHFDLRPYGILQMLDLIHPMYQHTAAYGHFGRK
                     PHKISYKDAKGKTQTATAFSWEKTDRADALRADAKIK"
BASE COUNT          222 a          440 c          457 g          264 t
ORIGIN      
        1 cccgacacct cgcgccgttg ctcgcgttcg cggcgcatgg cttgcagcgc gcgcacgcgg
       61 ccttcgttgc gcgtgcgccg cgccttgatg ccttggcgga tccacacttc ttcctgcgcc
      121 agtttcttgt cgaacagtgc gttcgcctgc gcctgcgcgt gcaggcgttc ctcgcgccga
      181 cgcagataat tttcgtagtc gcccggccac gccgtgacca cgcctcgatc tatttcgaca
      241 atccgcgtgg cgagcgcgcg caggaaactg cggtcgtggg tgacgaagac gaggctgaac
      301 ggcgccgcgc gcaaaaaatc ctccagccat tggatcgcgg cgacgtcgag gtggttcgtg
      361 ggttcgtcga gcagcaatac atccggcgcg cgcaccagcg cctgcgcgag cagcacgcgg
      421 cgcttcatgc cgccggacag cgcggcgaag tcggcgtcgg cgggaagttc caggcgttcg
      481 agcacctgcg acacgcggcg atccatgtcc caaccgtgct gcgcttcgat ctgtgcctgc
      541 acggcgccga gtgcggtcag atcgccgtgt tcggcaatca gatggtgata gcgcgcgagc
      601 agggcaccga gatcgccgag gccctgcgcg acgacgtcga acacgctgcc gcgcgcgtcc
      661 tgcggcactt cctgcgcgag cttcgcgacg cgcacgccgc cctgcacgcg cacctcgcca
      721 tcgtcgggtt tgatctcacc ggcgagcagg cgcagcagcg tcgacttgcc ttcgccgttg
      781 cggccgacga cgcagacgcg ttcgttgggc tcgatcgtca gatcgacctg ttcgagcagc
      841 agcggaccac cgacgctgta gtcgacgcgt tgaagttgca gcagtggcat gcgtgaattg
      901 taccggcaca aacaaaaacg ggccgcattg cgcggcccgt tggtgtacga caaggtaagg
      961 tgattacttg atcttcgcat ccgcgcgcag tgcatcagcg cggtcggtct tctcccacga
     1021 gaacgcggtg gccgtttgcg tcttgccctt ggcgtccttg tagctgatct tgtgcggctt
     1081 gcggccgaag tggccgtagg cagcagtgtg ttggtacatc ggatggatga gatccagcat
     1141 ctgcaggatg ccgtagggac gcaggtcgaa atgcttgcgg atcagctttt cgatcttgtc
     1201 gtcgctgatc ttgccggtgc cgaaagtggt gaccgaaatc gaagtcggct gcgccacgcc
     1261 gatggcgtag ctcacctgga tttcgcaacg gtccgcaatg ccggcggcga cgatgttctt
     1321 cgctacgtag cgcgcggcgt aggccgcgga acggtccacc ttggacgggt ccttgccgga
     1381 gaa
//
LOCUS       DASRVO010000616         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_70324, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000616 DASRVO010000000
VERSION     DASRVO010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70324"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1276)
                     /locus_tag="VFF05_07620"
     CDS             complement(92..1276)
                     /locus_tag="VFF05_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HZW51712.1"
                     /translation="MQRRHFLATLAAAPLAGFTGRVFAAPASSPRFLLVFLRGGYDCA
                     NLLVPYASDFYYESRPSIAIARPDSSTSGALRLDADWALSPAVREALEPLYTKKQIAF
                     VPFAGTPNLSRSHFETQDDIEHGLPENAHADRSGFLARLSGTLNGVATPIAFTSSLPL
                     SFQGGGDVPNISLARVGKAAFDARQSAVLAGMYKDTALAQPVAEGLALRQQVAGEFAD
                     DMMKASRGAVNAYGFTQQAPRIAQMMRDKYRLGFIDVGGWDTHVGEGGAQGALANNLG
                     NLARGLAAFAQAMGEDWKDTTVAVVSEFGRTFRENGNRGTDHGHGSVYWVLGGGIAGG
                     RVAGEQVKVAQPTLLQNRDFPVLNDYRGLLGGVFARLWGLNAAQLARVFPQARAEDLR
                     LV"
     gene            complement(1288..>2639)
                     /locus_tag="VFF05_07625"
     CDS             complement(1288..>2639)
                     /locus_tag="VFF05_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HZW51713.1"
                     /translation="QIGRHNFLDEQLAARDERLPPPIQAQIDAMPISHQTMADAIAKL
                     AAARDKLKTIDDAQAKAEERKALRKQGVALAVQAQERELLRAVYSPAQLKEQMVWFWL
                     NHFNVFDRKGPEAWLDADYVETAIRPHALGNFRDLVMATLTHPAMLVYLDNARNAAAH
                     HNENYARELMELHTLGVNAGYTQNDVQEMVRILTGVGVALNPRTPRLRPNQQALLIRH
                     DGLEFNPARHDFGDKVLLGHGIRGSGFDEVRQAVDLLVAQPACAHFISQELATYFVAD
                     DPPAPLVDAMARTFQRTHGDIRSVLRTLFDAPEFATSLGKKFKDPMHYLVSSVRLAYD
                     GREVPDPRRLVVWLNSQGEGEFAHQTPDGYPLVASAWDSSGQMSARFELARAMGRGDV
                     RRFGGVAMPQMPQISGALFVQAIKPWLSSTTLAALDQAKSPAEWNAYLLAAPESNYR"
BASE COUNT          435 a          814 c          912 g          478 t
ORIGIN      
        1 gcaagacgtc gggtcatggg atgctcctgc ggcttcgcaa gcataatccc ggcgagcgag
       61 atgtcgagtg aggcaggtct tggaccgttc ctcagaccag ccgcagatct tccgccctgg
      121 cttgcgggaa aacgcgcgcc agttgcgcgg cgttcaagcc ccacaaccgc gcaaacacgc
      181 cgccgagcag gccgcggtag tcgttgagca cggggaaatc gcggttctgc agcagggtcg
      241 gttgcgccac tttcacttgc tcgccggcga cgcgtccacc ggcgatgccg ccgccgagca
      301 cccagtacac cgaaccgtgg ccgtgatcgg taccgcggtt gccgttttcg cggaaggtgc
      361 ggccgaattc ggaaacgacg gcgacggtgg tgtctttcca gtcttcgccc atcgcctgcg
      421 cgaacgcggc gaggccgcgc gcgagattgc cgaggttgtt tgccagcgca ccctgcgcgc
      481 cgccttcgcc gacgtgggta tcccaaccgc cgacgtcgat gaagccgagc cggtacttgt
      541 cgcgcatcat ctgtgctatg cgcggcgcct gttgcgtgaa tccgtaggcg ttcaccgcgc
      601 cgcggctggc cttcatcatg tcgtcggcga attcgccggc gacctgctgg cgcagcgcga
      661 gtccttcggc aacgggttga gccagcgcgg tgtccttgta catgccggca agaaccgcgg
      721 attgacgcgc atcgaacgcg gccttgccca cgcgcgcgag ggaaatgttc ggcacgtcgc
      781 cgccgccctg gaaactcaag ggcagggaac tcgtgaacgc gattggcgtg gcgacgccat
      841 tgagcgtgcc ggacaggcgc gcaaggaatc cggaacgatc ggcatgggcg ttttccggca
      901 ggccatgttc gatatcgtcc tgcgtctcga agtggctgcg cgacaggttc ggcgtgcccg
      961 cgaacggcac gaaggcgatc tgctttttcg tgtacaacgg ttccagcgct tcgcgtaccg
     1021 cgggcgacaa tgcccagtcg gcgtccagac gcagcgcacc gcttgtcgag gaatccgggc
     1081 gtgcgatggc gatgcttggc cgcgactcgt agtaaaaatc gctggcatac ggtacgagca
     1141 ggtttgcgca gtcgtagccg ccgcgcagga acacgagcag gaagcgcggc gaggaggccg
     1201 gcgcggcgaa cacgcgtccg gtgaatccgg caagcggcgc ggcggcgagc gtggcgagaa
     1261 aatggcggcg ctgcatgggc gtatccttca gcggtaattg gattcgggcg cggcgagcag
     1321 ataggcattc cactctgctg gcgacttggc ttgatccagc gcggccagtg tcgtgctcga
     1381 cagccacggc ttgatcgctt gaacgaacag tgctccggag atctgcggca tctgcggcat
     1441 ggccacgccg ccgaaccggc gcacgtcgcc ccgacccatt gcgcgcgcca gttcgaaacg
     1501 tgcgctcatc tggccggacg aatcccaggc gctggcgacg agcggatagc cgtccggcgt
     1561 ctggtgcgcg aattcgcctt cgccttggct attcagccac acgacgagtc gacgcgggtc
     1621 cggcacttcg cgaccgtcgt aggccaggcg cacggacgag acgaggtagt gcatcggatc
     1681 cttgaatttc tttcccagcg aggtggcgaa ttcgggcgca tcgaacagcg tgcgcagcac
     1741 cgaacggatg tcgccgtgcg tgcgctggaa ggttcgcgcc atcgcgtcca ccagcggcgc
     1801 gggcggatcg tcggcaacga agtacgtcgc gagttcctgc gagatgaaat gcgcgcaggc
     1861 tggttgtgcg acgagcaggt cgacggcttg gcgcacctcg tcgaaaccgc tgccgcgaat
     1921 gccgtggccg agcagtacct tgtcgccgaa atcgtggcgc gcgggattga actccaatcc
     1981 gtcgtggcgg atgagcagcg cctgctggtt cggacgcaga cgcggcgtgc gcggattcag
     2041 cgccacgccg acgccggtga ggatgcgcac catttcctgc acgtcgttct gcgtgtagcc
     2101 ggcgttcacg ccaagggtgt gcagctccat cagctcgcgc gcgtagtttt cgttgtggtg
     2161 cgcagcggca ttgcgcgcat tgtcgaggta caccagcatc gccggatgcg tcagcgtggc
     2221 catgacgagg tcgcggaaat tgccgagcgc atgcggacga atagcggttt ccacgtaatc
     2281 ggcgtcgagc caggcctcgg ggcctttgcg gtcgaacacg ttgaagtggt tcagccagaa
     2341 ccagaccatc tgttccttga gctgggctgg cgaatacacc gcgcgcagca attcgcgctc
     2401 ctgcgcctgc acggcgagag cgacgccttg cttgcgcagc gccttgcgtt cttcggcctt
     2461 ggcctgcgca tcgtcgatgg tcttgagctt gtcgcgtgcg gcggcgagct tggcgatggc
     2521 gtcggccatg gtctggtgcg aaatcggcat tgcgtcgatt tgcgcttgaa tcggcggcgg
     2581 caggcgttcg tcgcgcgcgg cgagttgttc gtcgaggaaa ttgtggcggc cgatttgtt
//
LOCUS       DASRVO010000617         1644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_226462, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000617 DASRVO010000000
VERSION     DASRVO010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226462"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..>1644
                     /locus_tag="VFF05_07630"
     CDS             23..>1644
                     /locus_tag="VFF05_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW51714.1"
                     /translation="MNGTSLTVVGVARKGFDGVQLGETPDIFVPVTMKSQMMPTEGRP
                     LDAHDDFWLPVIARLNPGMNPVGAQAALQPVYRGILESDAKILKLSGQRLQRFLAKPL
                     LLVDGAHGRLVLQQDAQEPLLVLMGMVGLVLLVACANLAGLLVARGEARRREIAVRLT
                     SGASRWRLVRQLLTESLLVAIAGGAAGLALASWCLKAMVEAIPPDIGMAGLQAALDGR
                     VLWFAVAVTLLTSALFGLVPALRATRLNLQATLKDQGASVSEGRANVRLRQVLIVAQV
                     ALTAVLLTGAGLLARTLINLNHTNLGMRTDRVLQFSVAPDLNGETPSQTLQFANSASR
                     RIAALPGVRSATVSTMPMFSDDDMGFNITPEGYVIHSGDDMDVLADYVGPNYLSTLGI
                     PLMGGREFTEADTASSQKVCIINQKLAQRFFAGRNPIGLRVARGGGTPDIEIVGVAAD
                     SKWDGARSDIVPFMYMPYAQEARLGAFTFYVRTERDPAQMATSLRSAIAELDPNLPVN
                     NLRTLAEQVSESMFDGRLVALLSVSLALLAALL"
BASE COUNT          272 a          524 c          535 g          313 t
ORIGIN      
        1 gatcttgaac aagccgctga ttgtgaacgg cacttcgttg accgtggtgg gcgtggcgcg
       61 caagggattc gatggcgtgc aacttggcga aactccggac attttcgttc ctgtcaccat
      121 gaaatcgcag atgatgccga ccgagggccg tcccctcgac gctcatgacg atttctggct
      181 cccggtcata gcccggctca atccaggaat gaatcctgtt ggtgcgcaag ccgcgctgca
      241 accggtgtac cgaggcatcc tcgaatcgga cgcgaagatc ctgaaactct ccggccaacg
      301 cttgcagcga ttcctggcta agccattgtt gctggtggac ggcgcgcacg gccgcctggt
      361 cctacagcag gacgcacaag agcctctgct cgtgctgatg ggcatggttg gactggtgct
      421 gctggtcgct tgcgcgaatc tcgccggcct gctggtggcg cgcggcgagg cgcgccggcg
      481 cgagattgcc gtgcgcctga cctcgggcgc aagccgctgg aggctcgtgc ggcagttgct
      541 gacggaaagc ctgctggtcg ccattgccgg cggagcggca gggctggcgc tcgcttcgtg
      601 gtgcctgaag gccatggtcg aggccatccc gccggacatt ggcatggccg ggctccaagc
      661 cgcgctcgac gggcgcgtgc tgtggttcgc ggtggccgtg acgctgttga cgtcggcgct
      721 cttcggactg gttccggcgc tgcgcgccac gcgcctgaat ctccaagcca cattgaagga
      781 tcagggcgcc agcgtgtcgg agggccgcgc gaacgtgcgg ctgcggcagg tcctgattgt
      841 cgcgcaggtg gcgctgaccg ccgtgctgtt aacgggcgcg gggctgctgg cccgcacgct
      901 gatcaatctg aaccacacca atctcggcat gcgcaccgat cgcgtcctcc agttctcggt
      961 cgcgcccgat ttgaacggcg aaacgccgtc gcaaacgctg caattcgcca acagcgcgag
     1021 ccggcgcatc gccgcgctgc ccggagttcg ttcggccacc gtctccacca tgccgatgtt
     1081 ttccgacgac gacatgggct tcaacatcac gcccgaaggc tacgtcattc attcgggcga
     1141 cgacatggac gttctggccg actacgtcgg gcccaattat ctttcgactc taggcattcc
     1201 gctgatgggc ggccgcgaat tcaccgaagc agacaccgct tcgagccaga aagtttgcat
     1261 catcaatcaa aaactggcgc agcgcttctt cgccggccgc aatccgatcg gcctgcgcgt
     1321 cgcgcgaggc ggcggcacgc ccgacattga aatcgtcggg gtggcggccg acagcaaatg
     1381 ggacggcgcg cgcagcgaca tcgtgccgtt catgtacatg ccgtacgcgc aggaggcgcg
     1441 tttgggagcg ttcacgttct atgtgcgcac cgagcgcgat cccgcgcaaa tggcgaccag
     1501 cttgcgttcc gcgatcgccg aactcgatcc gaatctgccg gtcaacaacc tgcgcacgct
     1561 ggcggagcaa gtgagcgaat cgatgttcga tggacgcctg gtggccttgc tttccgtctc
     1621 cttggcgctg ctggcggctc ttct
//
LOCUS       DASRVO010000618         1266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_251871, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000618 DASRVO010000000
VERSION     DASRVO010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251871"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VFF05_07635"
     CDS             <1..1073
                     /locus_tag="VFF05_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51715.1"
                     /translation="MSNRSCLFYLGLLLLAPQACLHAEILVGGFGDGVTPEPLRGFSD
                     NANGNVAPLRVLGGPTTGLKTPVGGVYEPVENVIYVADFYGTAIRVFYANANGDAAPI
                     RVVGPTYVSQVRSIAVDVAHEELLITSTCCYKVFDRGANGDVFFKRYVQWGGLSGSVT
                     QQNSPSALVYMAPTDEVAETDTDSASPYTPKLLVFNRTDSGNTAPKRMLKGTNTQFGN
                     YASGLAYDAAARKLYIGAYTTNPDLSRSARVLVFDDSANGDVAPLRSIAGAATGLELS
                     ATSSIGGLAIDPEHQHLIVSVSDYNAAAGNKLLVFALGASGNTAPLQTIAGASTGLPK
                     SIGAPIWVSSERIFANGFEGAP"
BASE COUNT          245 a          415 c          382 g          224 t
ORIGIN      
        1 ccatgtcaaa tcgatcgtgc ctgttttacc ttggcctgtt gctgctggcg ccgcaggcct
       61 gcctccatgc cgaaatcctg gtcggcgggt tcggcgatgg tgttacaccg gagccactac
      121 gcggattctc cgacaacgcg aacggcaatg tcgcgccgct gcgcgtgctc ggcggtccaa
      181 ccaccggtct caagacgccg gtcggcggcg tttacgaacc ggtggagaac gtgatctacg
      241 tcgccgactt ttacggcacg gcgatccgcg tcttctacgc caacgccaac ggcgacgccg
      301 cgccgatccg ggtcgttggc cctacttatg tcagccaggt gcgcagcatc gccgtggatg
      361 tcgcgcacga ggaactgctc atcaccagca cctgctgcta caaggttttc gatcgcggtg
      421 ccaacggcga cgtcttcttc aaacgttacg tgcaatgggg cggcctgtcg ggcagcgtga
      481 cccagcaaaa ttccccaagc gccctggtgt acatggcgcc gaccgacgag gtggccgaaa
      541 cggataccga ctcggcgtcc ccgtatacgc cgaaactgct ggttttcaac cgcaccgaca
      601 gcggcaatac cgcgcccaaa cgcatgctca agggcacgaa tacgcaattc ggcaattacg
      661 ccagcggact ggcctacgat gccgctgcgc gcaagctgta tatcggcgcc tacacgacga
      721 atccggatct gagccgttct gcgcgggtgc tggtattcga cgactccgcc aacggcgacg
      781 tggcaccgct gcgcagcatc gccggcgccg cgaccggcct ggaactgtcg gccacgagta
      841 gtattggcgg gctggccatc gatccggagc atcagcacct gatcgtctcg gtaagcgact
      901 acaacgcggc cgccggcaac aaattgctcg tgttcgccct cggcgccagc ggcaacaccg
      961 cgccgctgca aaccatcgcc ggcgcatcga ccggcctgcc caagagtatc ggtgcgccga
     1021 tctgggtgtc ctccgaacgc atcttcgcta acggtttcga aggcgcgccc tgaaaaaagc
     1081 cccgcatcgc tgcggtcgaa cttcatgcac gcgatgcgcc accgcgcatt gcgcgcgtga
     1141 agtgaggttg aggcatggat gccgatcgga aggcgcacca gggaaggtct catggaaaaa
     1201 aaaagccccg cacttggcgg ggcttcgtgt ttctgcaacg ggaacggctt cgatcagaag
     1261 tccata
//
LOCUS       DASRVO010000619         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_95617, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000619 DASRVO010000000
VERSION     DASRVO010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95617"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1174)
                     /locus_tag="VFF05_07640"
     CDS             complement(<1..>1174)
                     /locus_tag="VFF05_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZW51716.1"
                     /translation="DDRGQEGLELSFDDWLAGKPGAKRVIRDNKGNTVENVELVREPK
                     PGRDLTLSIDRRIQYLAYRDLKAAIIDHHAKSGSMVVLDATNGEILAMANQPSYNPNA
                     PAADPAYRRNRAVTDVVEPGSTMKPFTIATALQSGKWTPDTPIDTNPGTFVLAGHIIR
                     DVANHGLLTVTGVITHSSNIGAAKIALTLPSEDMYDMDHRFGFGESTGSGFPGESPGL
                     LPPARSWGVIEKATIAYGYGLNVTTLQLAQAYAALADGGRLRQPTFVKGVRNPDVAVI
                     DPQLAHTIVKMLETVVAPGGTALRAAVPNYLVAGKTGTSRQAVAGGYESRYISLFAGM
                     APASNPRLVAVVVIHDPQGGYFGGTVSAPVFSQVMTGALRLLDVAPDNVQHWYAGGP"
BASE COUNT          184 a          369 c          408 g          213 t
ORIGIN      
        1 gggcccgccg gcataccagt gctgtacgtt gtccggagcc acgtcgagca ggcgtagtgc
       61 gccagtcatg acctggctga acaccggcgc cgaaaccgtg ccgccgaaat aaccgccctg
      121 cggatcgtgg atcaccacga ccgcgaccag gcgcggattg ctcgccggcg ccatgccggc
      181 gaacagcgaa atgtagcggc tctcgtaacc gccagccacg gcctggcgcg aagtgccggt
      241 cttgccggcg acaaggtaat tcggcaccgc cgcgcgcaac gccgtgcctc ccggcgcaac
      301 cacggtctcg agcattttca cgatggtgtg cgcgagctgc ggatcgatca cggcgacatc
      361 cggattgcgc acgcctttga cgaacgtggg ctggcgcagg cggccaccgt cggcgagcgc
      421 ggcataggcc tgggcgagtt gcagcgtcgt cacgttgagg ccgtaaccgt aggcgatcgt
      481 ggccttctcg atcacgcccc agctgcgcgc cggtggcagg agtcccggcg attcgccggg
      541 gaatccgctg cccgtggatt cgccgaaacc gaaacgatga tccatgtcgt acatgtcttc
      601 gctcggcagg gtcaacgcga tcttggccgc gccgatgttg ctcgaatgcg tgatcacgcc
      661 ggtgacggtg agcaggccgt ggttggcgac gtcgcggatg atgtgtccgg cgagcacgaa
      721 cgtgcccggg ttggtgtcga tcggcgtatc cggcgtccac ttgccgcttt gcaaggcggt
      781 ggcgatggtg aacggcttca tcgtcgaacc cggctcgacc acgtcggtca cggcgcggtt
      841 gcgccggtag gcgggatcgg cggccggcgc gttcgggttg tacgagggct ggttggccat
      901 cgcgaggatt tcgccgttgg ttgcatccag caccaccatc gaaccggact tggcgtgatg
      961 gtcgatgatc gcggccttga gatcacggta ggccaggtac tggatgcggc gatcgatgga
     1021 caaagtcagg tcgcgaccgg gtttcggctc gcgcaccagt tccacgttct cgacggtgtt
     1081 gcccttgttg tcgcggatga cgcgtttcgc gccgggcttg ccggccagcc agtcgtcgaa
     1141 cgacaactcg agtccttcct ggccgcggtc gtcg
//
LOCUS       DASRVO010000620         1614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_45098, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000620 DASRVO010000000
VERSION     DASRVO010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45098"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VFF05_07645"
     CDS             <1..602
                     /locus_tag="VFF05_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51717.1"
                     /translation="KDGQMIDGFMGALPEGQVREFLARHLAAPTEQPDAPAPVAAPET
                     TEQSIARLRKEIAAAPDKPELKLDLALALMQTGQADAAQAELDALPANLAEDARAKRL
                     RNQLDFARVLKDAPDVAALRSRIAANGDDHAARDLLGVRLLLDGHAAAGLDEFLHILK
                     TQRDWNDGAAKKRLIAAFSVLDDEELVGSYRRKMTSLLF"
     gene            686..>1614
                     /locus_tag="VFF05_07650"
     CDS             686..>1614
                     /locus_tag="VFF05_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51718.1"
                     /translation="MSDSTARALARRKLLVASVIAACALANDAFAVSVTDCGDSGAGT
                     LRGIIATAANNSTVDTSACSTITLSTGAIPVAQNTLTINGPVAGRTTITAIGSPVKDR
                     IFTHSGNLNFTLARLDIEYGRAYDGSLSNPGPGVNGGCIASSGNVFLTDSTVAHCSAN
                     SLHGRVYGGGIFASGVVWATRSEITGNLATSLSAYTYGGGVFSSGEFLAKYSTISNNV
                     ACPNAGCAGTGGGVDTRGGASISNSTISGNAARSSIGGVRLKSFSASPPTATISNSTI
                     SGNRAVTGAVGGVYANVPLTVRSSTIAFNTATSG"
BASE COUNT          298 a          556 c          500 g          260 t
ORIGIN      
        1 tcaaggatgg ccagatgatc gacggtttca tgggcgcgct gccggaaggc caggtgcgcg
       61 aattcctcgc ccgccatctc gccgcgccga ccgagcaacc cgacgctccc gctccggtcg
      121 ccgcgccgga aacgaccgag caatccatcg cgcgcctgcg caaggaaata gcggctgcgc
      181 ctgacaagcc ggaactgaaa ctcgatctgg cgctcgcgct gatgcagacc ggccaggccg
      241 atgccgcaca agccgaactc gacgccctgc ccgccaacct tgccgaggat gcgcgcgcga
      301 aacgcctgcg caatcagctc gatttcgccc gcgtgcttaa ggacgcgccc gacgtcgcag
      361 cgctccgctc gcgtatcgcc gcgaatggag acgaccacgc cgcgcgcgac ctgctcggcg
      421 tgcgcctgct gctcgacggc cacgccgccg ccgggctgga cgaattcctg cacattctta
      481 aaacccagcg cgactggaac gatggcgcgg caaagaagcg cctgattgcc gccttcagcg
      541 tgctcgacga cgaggaactg gttggcagtt accgcagaaa gatgacgtcg ttgttgttct
      601 agcggatctc gctgcgttct tcacggagtc tccacgtcgg ccttgcctac actttgccgc
      661 aacccaccgg gtttgggaga gtccgatgtc cgattcgacc gcgcgcgctc tggcgcgacg
      721 caaactgctc gtggcgtccg tgatcgctgc ctgcgcgctg gcgaacgatg cctttgcggt
      781 gtccgtcacc gactgcggcg acagtggcgc tggcacgctg cgtggcatca tcgcgaccgc
      841 cgcgaacaac agcacggtgg acacctccgc gtgcagcacg atcacgctga gcacgggcgc
      901 gatccccgtt gcccagaata cgctgacgat caacggcccc gtcgctgggc gcaccacgat
      961 cacggccatt ggcagtccgg taaaggatcg catcttcacc cacagcggca atctcaattt
     1021 cacgctcgcg cggctggaca tcgagtacgg ccgcgcctac gacggcagcc tgagcaatcc
     1081 gggacccggc gtgaacggcg gctgcatcgc ctcgtcgggc aatgtgttcc tgacggactc
     1141 aaccgtcgca cattgcagcg ccaacagcct gcacggtcgc gtctacggcg gcggcatctt
     1201 cgccagcggg gtggtatggg ccacgcgcag cgagatcacg ggcaacctcg cgacgtcgct
     1261 gagcgcatac acttacggcg gcggtgtgtt ttcaagcggc gaattccttg cgaaatacag
     1321 cacgatcagc aacaacgtgg catgccccaa cgcgggttgc gccggtaccg gcggcggcgt
     1381 ggacacgcgc ggtggcgcca gcatcagcaa ttcgacgatt tccggtaatg cggcgcgaag
     1441 cagtattggc ggcgtgagat tgaaaagttt ttccgcctcg ccgcccaccg ccacgatcag
     1501 caacagcacg atctccggca atcgtgccgt caccggcgcg gtcggtggcg tttacgcgaa
     1561 cgttccgctc accgtgcgca gcagcaccat cgcgttcaac acggcaacaa gcgg
//
LOCUS       DASRVO010000621         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_251921, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000621 DASRVO010000000
VERSION     DASRVO010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251921"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1542
                     /gene="alaS"
                     /locus_tag="VFF05_07655"
     CDS             <1..>1542
                     /gene="alaS"
                     /locus_tag="VFF05_07655"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZW51719.1"
                     /translation="GAPYASDNFWQMADTGPCGPCTEIFYDHGAQIPGGPPGSPDEDG
                     DRYIEIWNLVFMQFDRAADGTLSKLPAPCVDTGMGLERLAAVLQHVHSNYEIDLFQRL
                     IAKAAELTGTKDLENKSLRVIADHIRACSFLIVDGVLPSNEGRGYVLRRIIRRALRHG
                     YMLGQKQPFFHKLVPTLVAEMGEAYPELAAKQTTVEMALFAEEERFGETLEHGMKLFD
                     EIAARSGKSIPGVDAFRLYDTYGFPVDLTADIARERGLNVDMDGFEKAMNAQRERARA
                     ASQFETRASISADLVQRLKPTQFLGYGALDADALNVVGIVRDGREVQELRDGEEAMLL
                     LDATPFYAESGGQVGDVGELSNADGRFIVTDTIKLAGSFHGHVGKWSGKPLRVGAPLA
                     GSVDAARRQSTVLNHSATHLLHAALRQVLGEHVAQKGSLVAPDRLCFDFSHFKPMSHV
                     ELRKIEDIVNAEVRRNVEAEVHEMAYQEAIDFGAMALFGEKYGDRVRVLRMGDFSTEL
                     CGGTHV"
BASE COUNT          291 a          473 c          493 g          285 t
ORIGIN      
        1 ggcgcgcctt acgcctctga caatttctgg cagatggccg atacgggtcc ttgcgggccg
       61 tgcaccgaaa ttttctatga tcacggtgcg cagattcccg gcggaccgcc cggatctccc
      121 gacgaagacg gcgaccgcta catcgaaatc tggaacctcg tgttcatgca gttcgaccgc
      181 gccgccgacg gcacgctgtc gaaattgccg gcgccgtgcg tggataccgg catgggcctg
      241 gagcgcctcg ccgccgtgct gcagcacgtg cactcaaatt acgagatcga cctgttccag
      301 cgcctgatcg ccaaggccgc ggaactcacc ggcaccaagg atctggaaaa caagtcgttg
      361 cgcgtgatcg ccgatcacat acgcgcgtgc agttttctca tcgtcgacgg cgtgctgccg
      421 tccaacgagg gacgcggcta cgtgctccgc cggatcatcc gccgcgccct gcgccacggt
      481 tacatgctcg gccagaagca gccgttcttc cacaagctcg tgccgaccct ggtcgccgag
      541 atgggcgagg cgtatcccga gttggcggcg aaacagacga ccgtcgagat ggctctgttt
      601 gccgaggaag aacgcttcgg tgagacactg gaacacggca tgaagctgtt cgatgaaata
      661 gccgcgcgca gcggcaagtc gattcccggc gtcgatgcgt tccggctcta cgacacctac
      721 ggattcccgg tcgacctgac tgccgacatc gcgcgcgagc gtgggctgaa cgtagacatg
      781 gatggtttcg aaaaagccat gaacgcacag cgcgaacgtg cgcgcgcagc cagccagttc
      841 gagactaggg cgtcgatttc cgccgatctg gtgcaacgcc tcaagcccac gcagttcctg
      901 ggatacggcg cgctggatgc ggatgcgctg aatgtcgtcg gcatcgttcg cgatggccgc
      961 gaggtgcagg aattgcgcga tggcgaggaa gcgatgctcc tgctcgacgc cacgccgttc
     1021 tacgccgaat ccggcggcca ggtcggcgac gtaggcgaat tgagcaatgc cgatgggcgc
     1081 ttcatcgtca ccgacacgat caagctggcc ggctcattcc acggtcacgt tggcaagtgg
     1141 agcggcaagc cgttgcgcgt tggcgctccg ctggcgggtt ccgtagatgc ggccaggaga
     1201 cagtcgaccg tactcaatca ttcggcgaca catctgctgc acgccgcgct acgccaggtg
     1261 ctgggtgaac acgttgcgca gaaaggctcg ctggttgccc cggatcgcct gtgtttcgat
     1321 ttttcgcatt tcaagccgat gtcgcacgtg gaattgcgca agatcgagga catcgtcaac
     1381 gccgaggtgc gccgcaacgt tgaggccgaa gtccacgaga tggcctatca ggaggccatc
     1441 gatttcggtg cgatggcgct gttcggcgaa aaatacggtg accgcgtgcg cgtgctgcgc
     1501 atgggtgatt tttccaccga actgtgcggc ggcacgcacg tg
//
LOCUS       DASRVO010000622         1372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_70440, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000622 DASRVO010000000
VERSION     DASRVO010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70440"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VFF05_07660"
     CDS             <1..1185
                     /locus_tag="VFF05_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZW51720.1"
                     /translation="SRELTRRMIAALHAADGGRLTLLHGHRVTQLDRSAGKIGGAVAV
                     DERSGADVRIDAPVVVLAMGGINGSHAEVRANWPQGRAMPAAMLNGAHPFADGKLHHL
                     AAGLGAQITHAGEMWNYAAGFPHPYPHFEGHGLSTIPCKSALWLDHRGQRIGPPPLVT
                     GFDTHWLCQRVAEQEKPWTWHLLNWRIAIKEFAISGAEHNPRIRDMQFPQFLLETLFG
                     NRRLVRQMQRESPHFLVDETLAGLAAKMNALTASLDIDPVLLQATADAFDANFANGNK
                     LENDDQIRRILHARQWGPDRLRTCKPAPLQMRGAGPFIAIRMQLITRKSLGGLRTDLH
                     SRVLDVHDQPIPGLYCVGEAAGFGGGGASGKRSLEGTFLPGCILTARAAARSITGKEQ
                     LW"
     gene            1182..>1372
                     /locus_tag="VFF05_07665"
     CDS             1182..>1372
                     /locus_tag="VFF05_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZW51721.1"
                     /translation="MSLSYPATALDYRRLAQRRLPRFLFDYIDGGAGDEITLAANLAD
                     LAAIRLRQRVLVNVENVDTR"
BASE COUNT          226 a          471 c          450 g          225 t
ORIGIN      
        1 tcacgcgagc tgacgcggag gatgatcgcg gcgctgcacg cggccgacgg cggccggctg
       61 acattgctgc atgggcatcg tgtcacgcag ctcgatcgca gcgccggaaa gatcggcggc
      121 gccgtcgcgg tcgacgaacg atccggcgcg gatgttcgca tcgatgcgcc ggtggtggtg
      181 ttggcgatgg gtggcatcaa tggcagtcac gccgaggtgc gcgcgaactg gccgcagggc
      241 agggcgatgc ccgcggcgat gctcaacggc gcgcatccct tcgccgacgg caagctgcat
      301 catctggcgg cgggtctcgg cgcgcagatc acgcatgccg gcgagatgtg gaattacgcc
      361 gccggtttcc cgcacccgta tccgcatttc gaagggcacg gcctgtcgac gatcccgtgc
      421 aagtcggcat tgtggctgga ccatcgcggc cagcgcatcg gcccgccgcc gctcgtcacc
      481 ggcttcgata cgcattggtt gtgccagcgc gtggccgaac aggaaaagcc ctggacctgg
      541 cacctgctca actggcgcat cgcgatcaag gaattcgcga tctccggcgc cgagcacaac
      601 ccgcgcatcc gcgacatgca gttcccacag ttcctgcttg aaacgctgtt cggcaatcgt
      661 cgcctcgtgc ggcagatgca gcgcgagagc ccgcacttcc tcgtcgacga aaccctcgcc
      721 ggtctggcgg caaaaatgaa tgcgctgacc gcttcgctcg atatcgaccc ggtgttgttg
      781 caggcgaccg cggatgcctt cgacgccaac ttcgccaacg gcaacaagtt ggagaacgac
      841 gaccagatcc gccgcatcct gcatgcacgg cagtggggcc cggatcggct gcgcacctgc
      901 aagcccgcgc cgttgcagat gcgcggcgcc gggccgttca tcgccatccg catgcagctc
      961 atcacgcgca agagcctggg tggcctgcgc accgacctgc acagtcgcgt gctcgatgtc
     1021 cacgaccagc cgattcccgg cctgtattgc gtgggcgagg cggccgggtt cggcggtggc
     1081 ggcgccagcg gcaagcgttc gctggaaggc accttcctgc ccggttgtat ccttaccgca
     1141 cgcgccgcgg cgcgttcgat cacgggcaag gagcaacttt ggtgagcctt tcgtatcccg
     1201 ccaccgcact ggattaccgc cgtctcgcgc aacgccgcct gccgcgcttc ctgttcgact
     1261 acatcgacgg cggcgccggc gacgaaatca cgctcgcggc caacctcgcg gacctggccg
     1321 cgatccgcct gcgccagcgc gtgctggtca acgtggaaaa tgtggacacg cg
//
LOCUS       DASRVO010000623         1190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_70473, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000623 DASRVO010000000
VERSION     DASRVO010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70473"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFF05_07670"
     CDS             complement(<1..274)
                     /locus_tag="VFF05_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZW51722.1"
                     /translation="MTRGRRKALIVGAVGVAAAAAGALAALGLLRSQDSGAALQAARF
                     TDLEGKTRRLAEWRGKVVLCNFWATWCPPCRQEIPMLVALSKEMAPK"
     gene            complement(255..890)
                     /locus_tag="VFF05_07675"
     CDS             complement(255..890)
                     /locus_tag="VFF05_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51723.1"
                     /translation="MGRKGSRQNGTRARIAAAAARIMAEDGIDDFALAKRKAARQVGA
                     EETQALPANGEIEAELRAYRALYQPAEHPVRIAQLRRIALDAMRELRSFNPYLTGAVL
                     TGLAGRYAEIDLQLFPESAKDVEIFLIDRDLPYATSEGRRYSGDRAHSVTVLSLTWQG
                     APLRLSIFDPRDERLALKTTQAGRVAERAGLAEVGALIAADSHEENDARPT"
     gene            1008..>1190
                     /locus_tag="VFF05_07680"
     CDS             1008..>1190
                     /locus_tag="VFF05_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HZW51724.1"
                     /translation="MPAHLHILGVCGTFMGGIAAVAKAAGYRVTGCDANVYPPMSEQL
                     TALGIELIEDYDSAQIA"
BASE COUNT          197 a          411 c          366 g          216 t
ORIGIN      
        1 ctttcggtgc catttccttg ctcagggcga ccagcatggg gatctcttgg cggcacggcg
       61 ggcaccaagt cgcccagaag ttacacagca ctaccttgcc ccgccattct gccagccgcc
      121 gcgtcttgcc ttcgaggtcg gtgaatcgcg ctgcctgcaa cgcggcgccg gaatcctggg
      181 agcgcagcag tccaagtgcg gccagcgccc ccgccgcggc ggctgcgaca ccgactgcgc
      241 cgacaatcag cgccttacgt cggccgcgcg tcattctctt cgtgagagtc ggcggcaatc
      301 agcgcgccga cctcggccaa gccggcgcgt tcggccaccc ttcccgcctg cgtggtcttg
      361 agcgccagac gctcgtcgcg cggatcgaat atcgacagcc gcaaaggcgc gccctgccac
      421 gtcagagaca gaacggtcac cgagtgcgcc cggtcgcccg aatagcggcg cccttccgag
      481 gtcgcataag gcagatcgcg gtcaatcagg aagatctcca cgtccttggc gctctcggga
      541 aacaactgca ggtcgatttc agcgtagcgg ccggcaaggc cggtcaatac ggctccggtt
      601 aggtaaggat tgaagctccg caactcgcgc atcgcgtcga gcgcgattcg ccgcagttgc
      661 gcaatgcgca cggggtgctc ggctggctga tacagcgctc ggtaggcgcg caattccgcc
      721 tcgatctcgc cgttagcggg caatgcctgg gtttcctcgg cgccgacctg acgcgccgct
      781 ttgcgcttgg cgagcgcaaa atcgtctatg ccgtcctcgg ccatgatgcg ggccgcagct
      841 gcggcgatcc gtgcacgtgt tccattttgc cgcgatccct tgcggcccat tgccttgcct
      901 cgattcaaga gggagcctca cgttcggcct cccgtggtgt tagatcatag cgtaacctgg
      961 accgaacccc gcatcgcgta gaatcggcgg cttttcgccg cgcctccatg cccgctcacc
     1021 ttcacatcct cggcgtctgc ggcacgttca tgggtggaat cgcggccgtc gccaaggcag
     1081 ccggctaccg cgtcacaggc tgcgatgcga acgtatatcc gccgatgagc gagcagctca
     1141 ctgcgctcgg aatcgagctc atcgaggact acgacagcgc tcagatcgcg
//
LOCUS       DASRVO010000624         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_278915, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000624 DASRVO010000000
VERSION     DASRVO010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278915"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFF05_07685"
     CDS             complement(<1..687)
                     /locus_tag="VFF05_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HZW51725.1"
                     /translation="MRPRHNERHFTRRIGWLRAAVLGANDGIVSTASLILGVATAHAT
                     REAILTAGVASLVAGAMSMAAGEYVSVSSQSDMERAETEVERKELETDSAAEHKELAN
                     IYVTRGLTPSLANQVAGQLMAHDALDAHLRDELGISETLKARPLQAAFTSAVTYAAGA
                     ALPLASVIVAPPAYLLSVVPIVSLIVLTLLGGLAANAGGASIAKGSLRVLFWGAIAMA
                     VTAALGALFGA"
     gene            complement(691..1983)
                     /gene="xseA"
                     /locus_tag="VFF05_07690"
     CDS             complement(691..1983)
                     /gene="xseA"
                     /locus_tag="VFF05_07690"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZW51726.1"
                     /translation="MPESPAVQTTEREVFSPSQLVRLVRNLLEDAFPLIWVEGEISNF
                     VRAASGHMYFALKDTQAAVRCAMFKPRSTYLRFKPADGMRVLARVRVGLYEARGEFQL
                     VVEHLEEAGEGALLRAFAQLKAKLAAEGLFDPAVKRTIPKLPRRIGVVTSPSGAAIRD
                     VLSVIARRFPLAQVEVLPVPVQGKDAPPAIVTMLASASKARRHDVLLLTRGGGSLEDL
                     WAFNDEAVARAIRASSIPVVAAIGHEIDFTIADFAADLRAPTPSAAAELLVPDENEIR
                     RALDHVRARLDQWIGHCLHALAQRGDHALARLRTQHPRQRLRLGSERLGHLQARLRRQ
                     HPSTTLARQADALRVGIERLRTQLAQRLHAREMQLSELARALNAVSPLATLWRGYAIL
                     IDRDTGKVIRSVETSLRANRFSARLADGEIPLIRDPEH"
     gene            2084..>2851
                     /locus_tag="VFF05_07695"
     CDS             2084..>2851
                     /locus_tag="VFF05_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51727.1"
                     /translation="MSETSPLQAPREFSTGDRALRQRTQAFVRDWRASIGRQWQVEQV
                     GALYEELERLAELADAHGYADVADPVLELTAYLCSFVDRDSRPNSAQLQGLERLIERL
                     ADASGEKTARTARKTTAAVESTYRQVFYLRESAREIPGLAALLGQQGAVVRPFHERAS
                     LLRALDEASPDVVLVGEKFEADINAIVEAVQRLRPAHREPSLCLLLASETDPTRTLFV
                     QRAGADAVVVERDPIALSTQIDGLLAQRRALGYRVLIV"
BASE COUNT          474 a          915 c          996 g          466 t
ORIGIN      
        1 cgcgccgaac agcgcgccga gtgcggccgt caccgccatc gcaatcgcgc cccagaacag
       61 cacgcgcagt gaacccttgg cgatgctggc gccgccggca ttcgcggcga ggccaccaag
      121 caacgtcagc acgatcaacg acacgatcgg caccacgctg agcaggtaag cgggtggcgc
      181 cacgatcacg ctggccagtg gcaaggccgc gccggcggcg taagtgacgg cggaggtgaa
      241 cgcggcctgc aacggtcgcg ccttgagcgt ttcggaaatg ccgagttcat cgcgcaggtg
      301 ggcgtccagc gcatcgtgcg ccatcagttg gcccgcgacc tggttggcga gacttggcgt
      361 gaggccgcgc gtgacgtaga tgttcgccag ctccttgtgt tcggccgcgc tgtctgtttc
      421 gagttccttg cgctcgactt cggtctcggc ccgttccatg tcggattgcg aactgaccga
      481 cacgtattcg ccggcggcca tcgacatcgc gccggcgacg agactggcga cgcctgcggt
      541 caggatcgcc tcgcgcgttg cgtgtgcggt ggcgacgccg aggatcagac tggccgtgga
      601 gacgatgccg tcgttggcgc cgagcacggc ggcgcgcagc cagccgatgc ggcgggtgaa
      661 gtgacgctcg ttgtggcgag gacgcatcgg tcagtgctcc ggatcgcgga tcaagggtat
      721 ttcgccgtcg gcaagacgtg cgctgaagcg gttggcgcgc agcgatgtct cgacactgcg
      781 gatcaccttg ccggtgtcgc ggtcgatgag aatcgcataa ccgcgccaca gtgtggcgag
      841 cggactgacc gcgttcaatg ctcgtgccag ttcgctcaat tgcatttcgc gtgcgtgcag
      901 gcgctgcgcg agttgcgtgc gcaggcgctc gatgccgacg cgcaaggcat cggcctgtcg
      961 ggccagcgtc gtggacgggt gctggcggcg caggcgtgcc tgcaagtggc caaggcgctc
     1021 gctgcccagg cgcaggcgct gacgcgggtg ttgcgtgcgc aggcgggcca acgcatggtc
     1081 gccgcgctgc gccagcgcat gcaggcaatg tccaatccac tgatccagcc gcgcgcgaac
     1141 gtgatccagg gcgcggcgga tttcgttttc gtccggcacc agcaattccg ccgcggcgga
     1201 tggcgtaggc gcacgcaggt cggcggcgaa gtcggcgatg gtgaaatcga tctcatggcc
     1261 gatggccgcc acgaccggaa tcgaactggc gcggatcgcg cgcgcgacgg cctcgtcgtt
     1321 gaacgcccac aagtcttcga gcgaaccgcc gccgcgggtc agcagaagca cgtcgtggcg
     1381 acgcgctttc gacgcagatg cgagcatcgt cacgatggcc ggcggcgcat ccttgccctg
     1441 caccggcacc ggcaggactt ccacctgcgc gagcggaaag cgccgcgcga tcacgctgag
     1501 cacgtcgcgt atcgcggcgc cggagggcga ggtgacgacc ccgatgcggc gcggcaattt
     1561 cgggatcgtg cgcttgacgg cgggatcgaa caatccttcg gcggcgagtt ttgccttgag
     1621 ttgcgcgaat gcacgcagca aggcgccttc gccggcttct tccaggtgtt cgacgacgag
     1681 ctggaattcg ccgcgcgcct cgtacaggcc aacgcgcacg cgcgccagca cgcgcatgcc
     1741 gtcggcgggc ttgaagcgca gatacgtgct gcgcggtttg aacatcgcgc agcgcactgc
     1801 ggcttgcgta tccttgagcg cgaagtacat gtggccggac gcggcgcgca cgaaattcga
     1861 aatttcgcct tcgacccaga tcagcggaaa cgcgtcttcc agcaggttgc gcaccagacg
     1921 cacaagctga ctcggggaaa agacttcccg ctccgtggtc tggacggctg gcgattctgg
     1981 catgggaggc aacgaagtga ctggaagccg aggttactcc gcgcgccacg ggaatgccaa
     2041 acgggatttt cgcgccggca gggcgaacgg ctatagtagc cgcatgtcag agacttcacc
     2101 gttgcaggca ccacgcgaat tctccaccgg cgatcgtgcc ctgcgccagc gtacgcaagc
     2161 cttcgtgcgc gattggcgcg ccagcatcgg tcggcaatgg caggtcgagc aggtcggcgc
     2221 gctgtacgaa gaactcgaac gtctcgcgga actggccgat gcgcatggct acgcggatgt
     2281 cgccgacccg gtgctggaat tgaccgccta tctgtgctcg ttcgtggatc gcgacagcag
     2341 gccgaattcg gcgcagctgc agggcctgga acgcctgatc gagcgcttgg ccgacgccag
     2401 cggcgaaaag accgcgcgca ctgcgcgcaa gacgactgcg gctgtcgaat ccacttaccg
     2461 gcaggtgttc tatctgcgcg aaagcgcgcg cgagattccc ggattggccg cgctgctcgg
     2521 gcagcagggt gcggtcgtgc gccccttcca cgagcgggcc tccctgctgc gcgcgttgga
     2581 tgaggcgagc ccggacgtcg ttctggtcgg cgagaaattc gaggcggaca tcaacgcgat
     2641 cgtggaggcc gtgcaacggc tgcggccggc gcaccgcgag ccgtcgctgt gcctgctgct
     2701 ggccagcgag acggacccga cgcgcacgct gttcgtgcag cgcgctggcg cggacgctgt
     2761 cgtcgtcgaa cgcgatccca tcgcgttatc cacgcaaatc gacggacttc tggcgcaacg
     2821 ccgcgcgctc ggttaccgcg tactcatcgt c
//
LOCUS       DASRVO010000625         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_381622, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000625 DASRVO010000000
VERSION     DASRVO010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381622"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFF05_07700"
     CDS             complement(<1..415)
                     /locus_tag="VFF05_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZW51728.1"
                     /translation="MTPVNTRGRLYLVLMVLVLAATALIVRAVDLQVVRKDFYQQQGD
                     ARFLREVPIPVSRGTIFDRNGEPLAVSTPMMSIWANPAEVLDSDERIPALAQALGVDA
                     DGLKAQLAQRSDREFVYLRRQMAPAAAQAVLDLGIP"
     gene            complement(501..764)
                     /gene="ftsL"
                     /locus_tag="VFF05_07705"
     CDS             complement(501..764)
                     /gene="ftsL"
                     /locus_tag="VFF05_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZW51729.1"
                     /translation="MGAVIVLLLLAAVVSSAIGVVYARQQDRILFGELTRLTHEQDDL
                     NTEFGRLELEQATWAEPNRIEQIARGQLGMVNPGATDTVVVKQ"
     gene            complement(770..1696)
                     /gene="rsmH"
                     /locus_tag="VFF05_07710"
     CDS             complement(770..1696)
                     /gene="rsmH"
                     /locus_tag="VFF05_07710"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZW51730.1"
                     /translation="MPMANDTHAAVMLQETLEGLYVGDDGTYLDGTFGRGGHARALLE
                     RLGPRGRLLLMDRDPAAVAAARREFGADARVAIRHGNFAELADWDATLGGLDGVLLDL
                     GVSSPQLDDAGRGFSFRADAPLDMRMDPTSGESAAEFLARADEKEIADVLWNYGEERM
                     SRRIARAIVAQRRSAPIARTGELAELIAGIVGRPPSGKHPATRSFQALRIAVNGEMDA
                     LARGLRGAVTRLRAGGRLCVISFHSLEDRAVKQFLRDQSQAPLTRRGLPPPAPAALSL
                     RLIGHAQMPGAAELARNPRARSAVLRVAEKLG"
     gene            complement(1723..2169)
                     /gene="mraZ"
                     /locus_tag="VFF05_07715"
     CDS             complement(1723..2169)
                     /gene="mraZ"
                     /locus_tag="VFF05_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZW51731.1"
                     /translation="MFQGETAITLDDKGRLALPTAYREAVAKACANRLVFTYNPFEPG
                     CLWLFPFGEWEKVRDQINALSSVKRVHRDLQLKLVGAAATVELDGNARVLVPASQRGA
                     AGIEKKAVLLGMNAKFELWSEQAHLNKIRQSIGEHELSDEMLDLRL"
BASE COUNT          378 a          821 c          722 g          379 t
ORIGIN      
        1 cggggatgcc gaggtccagc acggcctgcg ccgcggccgg cgccatctgc cggcgcaggt
       61 agacgaactc gcggtccgag cgctgcgcca gctgcgcctt caggccgtcc gcatccacac
      121 ccagcgcctg cgccagcgca gggatgcgct cgtcgctgtc cagcacctcg gccggattgg
      181 cccagatcga catcatcggc gtcgacaccg ccagcggctc gccgttgcgg tcgaagatgg
      241 tgccgcgcga caccgggatc ggcacttcgc gcaggaaacg cgcatcgccc tgctgctggt
      301 agaaatcctt gcgcaccact tgcagatcga cggcgcgcac gatcagcgcc gtggccgcca
      361 gcaccagcac catgagcacg agataaagcc ggccgcgcgt gttcactggc gtcatgcgcg
      421 ttttcacgcg tgcgccatcg ccgtgcttcg tgcgcggcgg tcccggatgc gaggtcagca
      481 gcatgacggc ctccgcgcgc tcattgcttc accaccacgg tgtcggtggc gccgggattg
      541 accatgccga gctggccgcg cgcgatctgc tcgatgcggt tgggctcggc ccaggtcgcc
      601 tgctcgagtt cgaggcggcc gaattcggtg ttgagatcgt cctgctcatg ggtcaggcgc
      661 gtgagttcac cgaacaggat gcgatcctgc tggcgcgcgt agaccacgcc gatcgcactg
      721 ctcacgacag cggccagcag caacaggacg atgacggcgc cgatgagttt catccgagct
      781 tctccgctac gcgcagcacc gcgctgcgcg cgcgcggatt gcgcgcgagt tccgcggcgc
      841 cgggcatttg cgcgtgcccg atcaagcgca acgacaacgc agcaggtgcg ggcggaggca
      901 ggccgcggcg cgtgagcggc gcctgcgact ggtcgcgcag gaattgtttg accgcgcggt
      961 cttccagcga atggaagctg atgacgcaca ggcgtccgcc ggcacgcagc cgcgtcaccg
     1021 cgccgcgcag tccacgcgcg agcgcatcca tctcgccgtt caccgcgatg cgcagcgcct
     1081 ggaaactgcg cgtggccggg tgcttgccgg acggcggccg tccgacgatg ccggcgatca
     1141 gttccgccag ttcgccggtg cgcgcgatgg gcgcgctgcg ccgctgcgcg acgattgcgc
     1201 gcgcaatgcg ccggctcatg cgttcctcgc cataattcca taacacgtcc gcgatctctt
     1261 tctcgtctgc tcgtgccaga aactcggccg cgctctcgcc gctggtcgga tccatgcgca
     1321 tgtccagcgg cgcatcggcg cgaaagctga atccgcgccc ggcgtcgtcc aattgcggcg
     1381 aggacacgcc caagtccagc aacacgccat ccagcccgcc cagcgtcgca tcccaatcgg
     1441 ccaattcggc gaaattgccg tggcgaatcg ctacgcgcgc atccgcgccg aactcccgcc
     1501 gcgccgccgc caccgccgcc ggatcgcggt ccatcagcag caggcgaccg cgcggaccca
     1561 gccgttccag cagcgcacgc gcatgcccgc cgcgtccgaa cgtgccatcc aggtaggtgc
     1621 cgtcgtcgcc gacatacagt ccttccagcg tctcctggag catcaccgcg gcatgcgtgt
     1681 cattggccat cggcatcgtc caggcggcgc ggcacggcgc ggtcacaatc tcagatccag
     1741 catttcgtcc gacaattcgt gttcgccgat gctctggcgg atcttgttca ggtgcgcctg
     1801 ctcgctccac aattcgaact tcgcgttcat gccgagcagc accgccttct tctcgatgcc
     1861 ggccgcaccg cgctggcttg ccggcaccag cacgcgcgcg ttgccatcca gttccaccgt
     1921 cgccgccgcg ccgaccagct tgagctgcag gtcgcgatgc acgcgcttga cgctggacag
     1981 cgcgttgatc tgatcacgca ctttttccca ctcgccgaac gggaacagcc acaggcagcc
     2041 cggctcgaac gggttgtagg tgaacaccaa acgattcgcg cacgccttcg ccaccgcctc
     2101 gcgataagcg gtcggcaggg ccaggcggcc cttgtcgtca agcgtgatgg cggtttcacc
     2161 ctggaacatt tcccactaaa acccacgaaa tcccacttgc agcgaacagt agtccgcgcg
     2221 cgcaaccctg tcaaggaaaa atttgcttgc ggatcaatgg gaaagcgcag gttttagcga
     2281 atttcgagtg aattctcagg
//
LOCUS       DASRVO010000626         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_95754, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000626 DASRVO010000000
VERSION     DASRVO010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95754"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFF05_07720"
     CDS             complement(<1..316)
                     /locus_tag="VFF05_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HZW51732.1"
                     /translation="MMSSARSKHNAFAALELDRASDLREDENWLAEHARSAQARFLLL
                     DADGRALLSADRRHLFDIDHDAGKRLASSVPSFLGRVGPTVHFALALDAEAAARAAAE
                     TGG"
     gene            complement(327..686)
                     /gene="erpA"
                     /locus_tag="VFF05_07725"
     CDS             complement(327..686)
                     /gene="erpA"
                     /locus_tag="VFF05_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HZW51733.1"
                     /translation="MNETPDYQSLEAPLVFTDAAAHKVRELIAEESNPNLNLRVYISG
                     GGCSGFQYGFTFDEQRAEDDFAVVKDGVTLVVDPLSLQYLMGAEVDYTESLHGAQFVI
                     RNPNAKTTCGCGSSFAV"
     gene            complement(718..1128)
                     /locus_tag="VFF05_07730"
     CDS             complement(718..1128)
                     /locus_tag="VFF05_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZW51734.1"
                     /translation="MFNNNRKPANASADTTTLIASGTAIKGDVVFSGRLHVDGKVEGA
                     IRGDGAQAMLTLSEHAVVTGEVQAPHIVVNGAITGDVTASERLELASNARVEGNVYYK
                     LLEMSAGAQINGKMVHRAEPPKQLPKPEEKASSS"
     gene            complement(1115..1843)
                     /locus_tag="VFF05_07735"
     CDS             complement(1115..1843)
                     /locus_tag="VFF05_07735"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HZW51735.1"
                     /translation="MAKVLPPPILVVRTQRPARRWRWVFAVAVLWLASLAGALVLQHW
                     FVGVGEHVGATRAQKDHDAQLQKIAIAERAEQVARAANADLQQSLRDRQEEIAGLRAD
                     LAFYSKLTDGRSKPEGLAVHGVRMAASATPGVYNFTVTLTQTLKSGQVASGRVRLSVD
                     GIRAGKLATLAWTDLAPNQDASGLPFSFKYFQQMAGTVMLPAGFTPNRVHVEADAGGD
                     MGRADQDFTWSDALADQEPADVQQ"
     gene            1980..2402
                     /gene="hemJ"
                     /locus_tag="VFF05_07740"
     CDS             1980..2402
                     /gene="hemJ"
                     /locus_tag="VFF05_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HZW51736.1"
                     /translation="MPYLWIKALHVVFVVTWFAGLFYLPRLFVYHAESTDTAVRAQLK
                     IMERRLLVITHIGAALAIVFGFWTLMLAPAFLHQAWLHAKLGLVACLIFYHGYLVRLK
                     NRFARDEAPHTSRWLRIFNEVPALLLIAIVVLAVVKPG"
     gene            2494..>3062
                     /locus_tag="VFF05_07745"
     CDS             2494..>3062
                     /locus_tag="VFF05_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51737.1"
                     /translation="MHKQDDTQQAAELRLAFVRHLPKRLETLRKRGLRLCTQGWDINA
                     LSILFRELQTLAGACGRYGLLDVGERLFAMENFLAPFVQNVAIPNVAQTEAFTMQLAG
                     LDLLIAQHEERHIEPAVTQAAQLTVAPARAGSYPLQVTPPPEYWKRFGPAPARPASAS
                     APKPAMPPPVAVPAQKPPAPAIPTATAAVV"
BASE COUNT          506 a         1017 c          995 g          544 t
ORIGIN      
        1 caccgccggt ttccgcggca gcacgcgccg ccgcttcggc atcgagcgcg agcgcaaagt
       61 gaaccgtcgg gccgacgcgt ccgagaaaac tcggcacgct cgacgccaga cgtttgccgg
      121 cgtcgtgatc gatgtcgaac aaatgccgac ggtcggccga cagcagcgcg cggccatccg
      181 catcgagcag gaggaagcgt gcttgcgccg accgcgcgtg ttcggcgagc cagttctcgt
      241 cttcgcgaag atcgctggcg cgatcgagtt ccagtgcggc gaacgcgttg tgcttgctgc
      301 gcgcgctgga catcatgcgg cgacgctcac acggcgaacg acgacccaca tccgcatgtc
      361 gtcttcgcgt tcgggttgcg tatcacgaat tgggcgccat gcaggctttc ggtgtaatcg
      421 acttccgcgc ccatcaggta ttgcaggctg agcggatcga ccacgagcgt cacgccgtct
      481 ttgaccacgg cgaagtcgtc ttcggcacgc tgctcgtcga acgtgaatcc gtactggaag
      541 ccggaacagc cgccaccgga aatgtacacg cgcaggttga gattcggatt gctctcttcc
      601 gcgatcaact cgcgcacctt gtgcgcggcc gcgtcggtaa acacgagcgg ggcttcgagc
      661 gactggtagt ccggcgtttc gttcatgcgt gcaagatcgc gccgtggcgg gcggaattca
      721 agaggaggat gccttttcct cgggttttgg cagctgcttg ggcggctcgg cgcgatgcac
      781 catcttgccg ttgatctgcg caccggccga catttccagc aacttgtagt agacgttgcc
      841 ttccacgcgc gcattgctgg ccagttccag gcgttcggaa gcggtgacat cgccggtgat
      901 cgcgccgttg acgacgatgt gcggcgcctg cacttcgccg gtgacgacgg cgtgctcgga
      961 cagggtcagc attgcctgcg cgccatcgcc gcggatcgcg ccttcgacct tgccgtccac
     1021 gtgcaggcgc ccgctgaaga caacgtcgcc cttgatggcg gtgcccgacg cgatcagggt
     1081 cgtggtatcg gcggacgcat tcgcgggttt gcggttattg ttgaacatcg gcgggctcct
     1141 gatcggcaag cgcgtcggac caggtgaaat cctggtcggc gcggcccatg tcgccgccgg
     1201 cgtcggcctc gacatgaacg cggttggggg tgaatccggc gggcagcatc acggtaccgg
     1261 ccatctgctg gaaatacttg aatgaaaacg gcagacccga cgcatcctgg ttcggtgcga
     1321 gatcggtcca tgccaacgtc gccagcttgc cggcgcgaat gccgtcgacg gacagcctga
     1381 cgcggccgct ggcgacctgc ccggacttga gcgtctgcgt cagggtcacc gtgaaattgt
     1441 agacgcccgg cgtggcgctg gccgccatgc gcacgccgtg cacggcgagg ccctcgggct
     1501 tgctgcggcc atcggtgagc ttgctgtaga acgcgaggtc cgcgcgcagg ccggcgattt
     1561 cctcctgccg gtcgcgcagg ctctgctgca aatcggcgtt cgcggcgcgc gcgacctgct
     1621 cggcgcgttc ggcgatggcg attttctgca gttgggcgtc gtgatccttt tgcgcgcgtg
     1681 tcgcgcccac atgttcgccg acgccgacga accaatgctg caagacgagg gcgccggcga
     1741 gcgaagccag ccacagcaca gcgacagcga acacccaacg ccagcgccgc gcaggacgct
     1801 gcgtgcgcac cacgaggatg ggcggcggca aaaccttggc catgggcaat cgtgcgggag
     1861 cggcgcgtgg ccggcggaac catgtcttat agccgccgcc gcgcacgggc gtcaagcaag
     1921 cgggtcacga aaccacgcct gtgcgacaat cggcgcgtct cctgttcgcg gaatccgcca
     1981 tgccctatct gtggatcaag gccttgcacg tcgtcttcgt cgtgacctgg ttcgccggcc
     2041 tgttctacct gccgcgcctg ttcgtgtatc acgccgaatc caccgatacg gcagtgcgcg
     2101 cgcaattgaa gatcatggaa cgccgcctgc tcgtcatcac ccatatcggc gccgcgctcg
     2161 ccatcgtgtt cggattctgg acattgatgc tggcgccggc tttcctgcac caggcgtggc
     2221 tgcacgcaaa actcggcttg gtcgcctgcc tgatcttcta ccacggctac ctcgtacgct
     2281 tgaaaaaccg atttgcgcga gacgaggcgc cgcatacctc gcgctggctg cgcattttca
     2341 acgaagtccc cgcactattg ctgatcgcca tcgtcgtgct cgcggtggtc aagccgggct
     2401 gacctacggg ttcgatccaa cgtcgttttc cgccattcca ctcgtttatc gggcgccgcc
     2461 gaaccggtat agtgcggaca aacggcggaa cccatgcaca aacaggacga cacccaacag
     2521 gcggcggagc tgcggctggc tttcgtgcgg cacctgccca agcgcctgga gaccctgcgc
     2581 aagcgcggcc tgcgcctgtg cacgcagggc tgggacatca acgcgctgtc gatcctgttc
     2641 cgcgaattgc agacgctggc cggtgcctgc ggccgctacg gcctgctcga cgtcggcgaa
     2701 cgcctgttcg cgatggaaaa tttcctcgcg ccgttcgtgc agaacgtcgc gatcccgaat
     2761 gttgcgcaga ccgaagcttt cacgatgcag ctggcaggcc tggacctgct gatcgcgcag
     2821 cacgaggaac gccacatcga gccggcggtg acgcaggccg cgcagctcac cgtcgcgccc
     2881 gcgcgcgccg gcagctatcc gttgcaggtg acgccgccgc cggaatactg gaagcgcttc
     2941 ggacccgcgc ccgcacggcc ggcttcggca agcgcaccga aaccggcaat gccgccgcct
     3001 gtcgcggtgc ccgcgcaaaa accgccggcg ccggcgatac caacggcgac cgcagccgtc
     3061 gt
//
LOCUS       DASRVO010000627         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_95767, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000627 DASRVO010000000
VERSION     DASRVO010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95767"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..471
                     /locus_tag="VFF05_07750"
     CDS             49..471
                     /locus_tag="VFF05_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZW51738.1"
                     /translation="MLERLSEMLFIDAVRQYLNTLPEESNGWLAGLRDRFVGRALTLL
                     HAAPAQPWTVAELGRRVGLSRSAFHERFAGMIGQTPMQYLARWRLQAGAALLRDTAAT
                     IAAVAQEVGYESEASFSRAFKRFVGTPPARWRREQAQN"
     gene            679..1236
                     /gene="lexA"
                     /locus_tag="VFF05_07755"
     CDS             679..1236
                     /gene="lexA"
                     /locus_tag="VFF05_07755"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZW51739.1"
                     /translation="MPRPPRHGGRRPGAGRKPGSGLYGEPTTILRIPQSRLATVRAFL
                     DQCRQGKRASLPAANDELVIPLIGRVAAGQPIDADAHVEREIAIDRFLFRPRPDYLLR
                     VHGHSMRDAGIFDRDLIAVHRTPEARNGQIVVARLDGEITVKRLSRQRNRIVLLPENP
                     DFEPITVAADDFAIEGIYVGVIRVS"
     gene            1236..1847
                     /gene="imuA"
                     /locus_tag="VFF05_07760"
     CDS             1236..1847
                     /gene="imuA"
                     /locus_tag="VFF05_07760"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HZW51740.1"
                     /translation="MSAVAISDVLRDPRVWQGPRHAQPQVEPTGHAALDAALPGGGLP
                     VGSLVEVLHEFDGTSEMTVLLPLIARVSRTQHRIVFVNPPYIPYAPAFATAGVDPACM
                     HTIQNCGDESLWSAEQALRSSTCALVACWPRLASDRQLRRLQLAAEGGRAIGIVFWPA
                     RMAMTTSPAAVRLRAGVRGIEVLKCRGGNARPYAIPLSSTARC"
     gene            1855..>3239
                     /locus_tag="VFF05_07765"
     CDS             1855..>3239
                     /locus_tag="VFF05_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HZW51741.1"
                     /translation="MQWAAILLPDLVLDAVRRSGADPDSPIALIDGPPQRRIVQSCSP
                     RAREAGVHAGQALAAARALLPNLIALPDDPRRTMSLRDLLVAWAYGYSGDVAWLGDNA
                     LVLEVGGSLGLFGPWTDFERRLRRDLVALGVTHRIALAPLPRAACVFAGCRDGFGVCA
                     DMAMRQALRRVPLSLARLPDVSLLASMGLRTLGEVLDLPRAGLARRCGEAFVEVLDCL
                     IGSIPDPLPRYRPPDRFEAKIEFDAELRSMEGLQFPLKRLIGDLCLFLAARDGGVDTI
                     EIGFEHDERAPTSITLRSPAPERDAAQWFNLVRLRLERLQLPAPVCGVQLVAKELPPF
                     VPIARNLFDSASTAALTWPQLVARLEARLGDGAVRTFYPAADHRPEAALTTDASASGR
                     IVTGPRPVWLLPKPIPLRDRAPRIVAGPERIESGWWDGREASRDYYVIETSVGQTAWA
                     FRPAGAVDGPWL"
BASE COUNT          477 a         1116 c         1060 g          586 t
ORIGIN      
        1 cgtcaggccg cgctggaagc ttcgagcctg cggccgggcg gcggcgcgat gctggaacgc
       61 ctgagcgaaa tgttgttcat cgacgccgtg cggcaatacc tgaacaccct gcccgaagaa
      121 agcaacgggt ggctggcggg gctgcgcgac cggtttgtgg ggcgggcgtt gacgctcctg
      181 cacgccgcgc ctgcgcaacc atggaccgtg gccgagttgg gccgccgcgt cgggctgtcg
      241 cgctccgcat ttcacgagcg ctttgcgggc atgatcggcc agacgccgat gcagtatctc
      301 gcccgttggc gcctgcaagc cggagccgcg ctgctgcgcg acaccgccgc gacgattgct
      361 gccgtggcgc aagaggttgg ctacgaatcg gaagcgtcgt tctcgcgggc cttcaagcgc
      421 ttcgtgggga cgccgccggc acggtggcgg cgagagcagg cgcagaattg acgagacggc
      481 cggccggcgg atcccgagtc tgtccggcgt ccggcaggaa agggccggga ttcgctccac
      541 cgtcgtgaca gcgatgcatc cgtcttgaac cggttctggg gttcgatggt gtccgaatcc
      601 ctcgtccatt caatgcgtcg caatccctgc gacggatggt gctagacttg atttcagtaa
      661 ctgtaatcaa ggtgcaccat gcctcgtcct ccccgccacg gcggccgccg tcccggcgcc
      721 ggccgcaagc ccggctccgg cctctacggc gaaccgacga cgatcttgcg catcccgcaa
      781 agccgcctcg ccacggtccg cgcatttctc gaccagtgcc ggcaaggtaa aagggcttca
      841 ctgccggccg ccaacgatga actggttatt cccctgatcg gccgcgtggc ggccggccag
      901 ccgatcgatg cggatgcaca cgtggagcgc gagatcgcca tcgaccgctt cctgttccgc
      961 ccccgcccgg attacctctt gcgcgtgcac ggccacagca tgcgcgatgc cgggatcttc
     1021 gatcgcgatc tcatcgccgt gcaccgcacg cccgaggcac gcaacggcca gatcgtggtc
     1081 gcgcgcctgg atggggagat cacggtcaag cgcctctcgc gtcagcgcaa ccgcatcgtg
     1141 ctgttgcccg agaacccgga cttcgagccg atcacggtcg ctgccgacga cttcgccatc
     1201 gaaggcatct acgtgggcgt gatccgggtg tcgtgatgag tgccgtcgcc atttcggatg
     1261 ttttgcgcga tccgcgcgtc tggcagggac cacggcatgc ccagccgcag gtcgaaccga
     1321 ccgggcatgc ggcgctcgat gccgccttgc ccggcggcgg cctgcccgtc ggctcgctgg
     1381 tcgaagtgct gcacgagttc gatggcacct cggagatgac cgtgctgctg ccgctgatcg
     1441 cgcgtgtgtc tcgcacgcaa caccgtatcg tgttcgtcaa tccgccctac attccctacg
     1501 caccagcctt cgctaccgcg ggtgtcgatc cagcgtgcat gcacacgatc cagaactgtg
     1561 gcgatgaatc cctgtggtcc gctgaacaag ccttgcgcag ttccacgtgt gcgctggtcg
     1621 cctgctggcc ccgactggcc agcgaccggc aactgcgccg cctgcaactg gcggccgagg
     1681 gcggacgcgc gatcggcatc gtattctggc ccgcacgcat ggcgatgacg acttcgcccg
     1741 ctgccgtgcg cctgcgtgcc ggtgtccgag gcatcgaagt gctcaagtgc cgcggcggca
     1801 atgctcgacc ctacgccatt ccactctcgt ccaccgcacg atgctgagat cgccatgcag
     1861 tgggccgcga tacttctgcc ggatctggta ctggatgccg tccgccgctc gggcgccgat
     1921 cccgactcac ccattgcgct gatcgacggg ccgccgcagc ggcgcatcgt gcagtcctgt
     1981 tcgccccggg cacgcgaggc cggtgttcat gccggccaag ctttggccgc cgcacgcgca
     2041 ctgctgccca acctcatcgc cctgccggac gatcccaggc gcacgatgag cttgcgcgat
     2101 ctgctcgttg cctgggccta tggctacagt ggcgatgtcg cctggcttgg ggacaacgcc
     2161 ctcgtgctcg aagtcggcgg cagccttgga ctgttcggtc catggacgga cttcgaacgc
     2221 aggttacgcc gtgatctcgt tgcactgggt gtcacgcacc gcatcgcact ggcgccactg
     2281 ccgcgcgcag cctgcgtgtt cgccggatgc cgcgacggct ttggcgtctg cgcagatatg
     2341 gccatgcgtc aggcgctgcg acgcgtgcct ctttccctcg cccgcctgcc cgacgtatcg
     2401 ctgctcgcct cgatgggact gcgcaccttg ggcgaggtgc tcgacctgcc gcgtgccgga
     2461 ctcgcacgcc gctgcggcga ggctttcgtc gaggtactgg attgcctcat cgggagcatt
     2521 cccgatccgt tgccgcgcta tcgtcccccg gatcgcttcg aggcgaagat cgagttcgat
     2581 gcggaactgc gctcgatgga aggtttgcag tttccgctca agcgcctgat cggtgatttg
     2641 tgcctgttcc ttgccgcacg cgatggcggc gtggacacga tcgagatcgg cttcgagcac
     2701 gatgaacgcg cgccgacatc gatcaccctg cgctcgccag cgcccgagcg cgacgccgcg
     2761 caatggttca atctggtgcg cctgcgcctg gaacgcctgc agttgcccgc gccggtttgc
     2821 ggtgtgcagc tcgtggccaa ggagttgccg ccgttcgtgc ccatcgcgcg caacctgttc
     2881 gatagcgcat ccaccgcggc actgacctgg ccgcaactcg tggcaagact tgaggcgcgc
     2941 ctgggcgatg gcgcggtcag gacgttttat cccgccgccg accaccgacc ggaagcggca
     3001 ttgacgacgg acgcatccgc ctcgggtcgg atcgtcaccg gtccgcgtcc ggtgtggctg
     3061 ttgccaaaac ccattccctt gcgcgatcgc gcgcctcgca tcgtcgcggg acccgagcgc
     3121 atcgagtcgg gctggtggga tggccgcgag gccagccgcg actactacgt aatcgaaacg
     3181 agcgtcggac aaacggcctg ggcgtttcgt cccgcgggcg ccgtcgatgg gccatggct
//
LOCUS       DASRVO010000628         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_226752, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000628 DASRVO010000000
VERSION     DASRVO010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226752"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2082)
                     /locus_tag="VFF05_07770"
     CDS             complement(<1..>2082)
                     /locus_tag="VFF05_07770"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZW51742.1"
                     /translation="EVADAERQAWLAVNCVDDADLRDEVERLLAAQREPDPLLSGDAQ
                     SVIAEWSPDEDDASEVAPGTRIGAYRLLRVIGAGGMGRVYLAERADGQFTQQVALKRI
                     RGAVLTPELRQRFQREREVLARLAHPHIAQLHDGGVDDEGMPYFTLEYVDGQPMTAWC
                     DVRRLELRARAALLLKICDAVQYAHGHLVVHRDIKPSNILVSGDGEPKLLDFGIAKPL
                     DDALGSTLTGSQAQPMTREFAAPEQVLGEPATTATDVYALGVLFYLLLCGRMPYRGAA
                     LGTASWNKAILQDAPEPMAAAILRTPADAEAIATARASTPAALQRALRGDPERIVRRA
                     LAKQPASRYPTVDALAADLRAWLDGRALAGGTRTYRLRKFVRRHWLPLGAAAALLATI
                     LGGAAIMLADARRLAREAQTTSAVKDFLLDLFTAVDPHLSKGRSVDARKLLDRGAARI
                     AQDSRLDALQLAEIKATLGRIYYQLGVFDRADALQAQALAALAGRADAVLLRARTQAE
                     RALTLVELGNLKASGELADSARRSIDSRPDSNLADRIAAIHAQASAAIAQRDFAKVER
                     YANEELPLARQIAVRQPKPLYHALMSAGVASWGLHEYDTALARWREAVDVASHGKDLD
                     ELDTAIARINVSMALAEQSHYGQASEVAQQALAVEDKELGSDHPTVLNARRSLALYTF
                     HQGRYREARALL"
BASE COUNT          356 a          722 c          651 g          353 t
ORIGIN      
        1 agcagcgccc gtgcctcgcg gtaacgaccc tggtgaaagg tatacagcgc cagactgcgg
       61 cgggcattca gcaccgtcgg atggtcgctg ccgagttcct tatcctcaac cgcaagcgcc
      121 tgctgcgcga cttcgctggc ctgtccgtag tgcgattgtt cggcgagtgc catcgacaca
      181 ttgatacgcg cgatagcggt gtccagttcg tcgagatcct tcccatgcga ggcgacgtcg
      241 actgcctcgc gccaacgcgc cagcgcggtg tcgtattcgt gcaatcccca actcgccacg
      301 cccgcgctca ttaaggcgtg atatagcggc ttcggctggc gcacggcgat ctgccgcgca
      361 agcggcaact cctcgttcgc ataccgctcg acctttgcga aatcgcgctg cgcaattgcg
      421 gccgatgcct gcgcatgaat cgcggcgatg cgatccgcga gatttgaatc cggcctcgaa
      481 tctatgctgc gtcgcgcgct gtcggccaac tccccggaag ccttaagatt gccgagttcg
      541 accagtgtca gcgcgcgctc ggcctgagtg cgcgcccgca gcagcaccgc gtcggcgcgc
      601 ccggccaaag cggcgagcgc ttgcgcctgc agcgcatcgg cacgatcgaa tacaccgagc
      661 tgatagtaaa tgcgccccag ggtggccttg atttctgcta gctgcagcgc atcaagtcgg
      721 gagtcttgcg cgatgcgcgc cgcaccccga tcaagcaact tgcgcgcatc cactgacctg
      781 cccttggaca aatgcggatc gaccgcggtg aacaagtcga gcaaaaagtc cttcacggct
      841 gatgtggtct gcgcctcgcg cgcgagccgg cgcgcatccg cgagcatgat tgccgcgcca
      901 ccgaggatgg tcgcgagcaa ggccgccgcc gcgcccagcg gcagccagtg ccggcgcacg
      961 aatttgcgca atcgatacgt gcgcgtaccg ccagccagcg cgcgaccgtc cagccaagcg
     1021 cgcaggtccg cggccagtgc gtcgactgtt ggatagcgcg atgcaggctg tttggccaag
     1081 gcgcgccgca cgatgcgttc gggatcgccg cgcaaggcgc gctggagcgc cgccggcgtg
     1141 ctcgcgcgcg ccgtggcgat tgcttccgca tctgccggcg tgcgcaagat cgccgcggcc
     1201 atcggctcgg gcgcgtcttg cagaatcgcc ttgttccagc tcgcggtgcc cagtgccgcg
     1261 ccgcgatacg gcatgcgacc gcacagcaaa agataaaaaa gcacgcccaa cgcatacaca
     1321 tccgtggccg tggtcgcggg ctcgccaagc acttgttccg gcgcagcgaa ctcgcgcgtc
     1381 atcggctgcg cctgcgagcc ggtcaaggtc gagccgaggg cgtcatccaa cggtttggcg
     1441 atgccgaaat ccagcaactt cggttcgccg tcgccggaca ccagaatgtt cgagggcttg
     1501 atgtcgcgat gcacaaccaa gtgaccgtgc gcgtactgca ccgcatcgca aatcttcagc
     1561 agcaatgctg cgcgcgcgcg cagctccaga cgtcgcacgt cgcaccacgc cgtcatcggc
     1621 tgaccgtcga cgtattccag ggtgaaatac ggcataccct cgtcgtccac gccgccgtcg
     1681 tgcagttgtg cgatatgcgg atgcgccaag cgcgccagca cttcgcgttc gcgctggaac
     1741 ctctggcgca attccggggt gagcacagcg ccgcgaatgc gcttgagcgc cacctgctgc
     1801 gtgaactgac cgtccgcgcg ctcggccaga tacacccggc ccatgccacc ggcgccgatc
     1861 acgcgcaaca gccggtaggc gccgatccgc gtgccgggcg cgacttcgct cgcgtcgtcc
     1921 tcatccggcg accattccgc gatcacgctc tgtgcatcgc cggaaagtag cgggtctggt
     1981 tcacgttgtg cggcgagtag acgttctacc tcgtcgcgca ggtcggcgtc atcgacgcag
     2041 ttgaccgcca gccaggcttg ccgctcggca tccgcaacct cc
//
LOCUS       DASRVO010000629         1153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_252138, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000629 DASRVO010000000
VERSION     DASRVO010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252138"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VFF05_07775"
     CDS             complement(<1..366)
                     /locus_tag="VFF05_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HZW51743.1"
                     /translation="MSDALDLTAAEADARARDAADALASFRDEFLIPPHGDGEQTYLC
                     GNSLGLQPRATRQALIDELDDWRDLGVEAHFRGKHPWMPYHNEVRAHLAELVGALPSE
                     VVAMNSLTVNLHLMMASFYR"
     gene            complement(374..901)
                     /locus_tag="VFF05_07780"
     CDS             complement(374..901)
                     /locus_tag="VFF05_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51744.1"
                     /translation="MRKSALATLATILLAAGPAFAQSTGEFSLDQGIIKFSAPPAWPV
                     IMQKTEGDPQFVAFQVKDPADAGSGEATRVTVETRLLNDSSNFQPMVNRDVDKAKQMP
                     GYEQKTEGVDATTLRYFANSGKTKYEYRETWYLNSHLLVHVRCARPMLDKTTAQWTAD
                     YEKGCTQIMQSLKPR"
     gene            complement(919..>1153)
                     /locus_tag="VFF05_07785"
     CDS             complement(919..>1153)
                     /locus_tag="VFF05_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZW51745.1"
                     /translation="MDRLYFDSCVHDDRALRYLLDVAGARRVMLGTDYPFPLGEQDPG
                     AGIAALGLAPAEQESLYHGTALEWLGLPASRFA"
BASE COUNT          191 a          348 c          397 g          217 t
ORIGIN      
        1 ccggtagaag ctcgccatca tcagatgcag gttgacggtg agcgaattca tcgccacgac
       61 ttcgctgggc agcgcgccga cgagttcggc cagatgggcg cgtacctcgt tgtggtaggg
      121 catccacgga tgcttgccgc ggaaatgcgc ctcgacgccg aggtcgcgcc agtcgtcgag
      181 ttcgtcgatc aatgcctgcc gcgtcgcgcg cggctgcagg ccgagcgaat tgccgcagag
      241 gtaggtctgt tcaccatcac cgtgcggcgg aatcaaaaac tcatcacgaa aagacgcaag
      301 cgcatcggcg gcgtcgcgcg cgcgggcgtc tgcctcggcg gcggtaaggt cgagggcgtc
      361 actcatcgcc gcgtcaacgg ggtttcagcg actgcatgat ctgcgtacat cccttttcat
      421 aatccgccgt ccactgcgcc gtggtcttgt ccagcatcgg ccttgcacag cgcacgtgca
      481 caagcaggtg gctgttgagg taccaggttt cgcggtattc gtatttcgtc ttgccgctgt
      541 tcgcgaagta gcgcagcgtg gtggcatcca cgccctcggt cttctgttcg tagcccggca
      601 tctgcttggc cttgtcgacg tcgcgattga ccatgggctg gaagttgctg gaatcgttga
      661 gcaggcgcgt ctccacggtc acgcgcgtag cctcgccgct gccagcgtcg gcgggatcct
      721 tgacctggaa cgcgacgaac tgcgggtcgc cttcggtctt ttgcatgatt accggccacg
      781 ccggcggcgc gctgaacttg atgatgccct ggtccagcga aaactcgccg gtggactgcg
      841 cgaaggcggg gccggcagcg agcaggatcg tggcgagggt ggcgagtgcg gatttgcgca
      901 tgttcgtctc cagtggattc aagcgaaacg cgatgccggc aggccgagcc attccagcgc
      961 ggtgccgtgg tacagggatt cctgctcggc gggtgcgaga ccgagcgcgg cgatgccggc
     1021 gccgggatcc tgttcgccga gcgggaacgg atagtcggtg ccgagcatga cgcgccgcgc
     1081 gccggcgacg tcgagcaggt agcgcagcgc gcgatcgtcg tgcacgcagg aatcgaaata
     1141 caggcgatcc atg
//
LOCUS       DASRVO010000630         1258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_45336, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000630 DASRVO010000000
VERSION     DASRVO010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45336"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VFF05_07790"
     CDS             <1..1049
                     /locus_tag="VFF05_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW51746.1"
                     /translation="KVTHSATAYQEKFDHASREFSRHLAAGETLSQLPMYLVEAVGYS
                     GLIVIALVLMLQRGDIAHVLPALGLYGFAAYRMLPAMQVMYRGFAKLRFSLPTLDAIH
                     RDLSLPEIPASTTMQALAPRTEIRLSGIRYAYPATPDKPVLDGFDLAIPVGASIGIAG
                     RTGAGKSTVMDILLGLLQPQAGKLSVDGTVIDAGNVAAWQRAIGYVPQHIYLADASIR
                     ENIAFGVAPTDIDMAAVERAARAAQIHDFIAGDLERGYATRIGDRGIRLSGGQRQRVG
                     IARALYRDPPVLFFDEATSALDTQTEEAVNDAIRSLSGAKTIVVIAHKETSLRDCARV
                     VTLAPAPRAAAAAP"
     gene            1053..>1258
                     /locus_tag="VFF05_07795"
     CDS             1053..>1258
                     /locus_tag="VFF05_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51747.1"
                     /translation="MANARFPNLFIVGAPKCGTTALSHYLAGHPMIFMSEQSGAKEPN
                     YYCPDLNHPLVMFPYCIRSIEGYL"
BASE COUNT          218 a          448 c          377 g          215 t
ORIGIN      
        1 tcaaggtcac gcactccgcc acggcctacc aggaaaaatt cgaccacgcc tcgcgcgagt
       61 tctcgcgcca cctcgcggcc ggcgaaacgc tcagccagtt gccgatgtac ctcgtcgaag
      121 ccgtcggcta cagcggtctg atcgtgatcg ccctggtact gatgctccag cgcggcgaca
      181 tcgcccacgt gctgccggcg ctgggtctgt acggtttcgc cgcctatcgc atgctgccgg
      241 cgatgcaggt catgtaccgt ggattcgcca agctgcgctt ttccttgccc acgctggatg
      301 cgatccaccg cgacctgagt ctgccggaga tcccggccag cacgaccatg caagcgctcg
      361 cgccgcgcac cgaaatccgc ttgagcggaa tccgctacgc ctatccggcc acgcccgaca
      421 aacccgtgct ggatggcttc gacctcgcca tcccggtcgg cgccagcatc ggcatcgccg
      481 gtcgcaccgg cgccggcaag agcacggtga tggacatcct gctcggattg ctgcagccgc
      541 aggcgggcaa gttgagcgtg gacggcaccg tcatcgacgc cggcaacgtc gcagcgtggc
      601 agcgcgccat cggctatgtg ccgcagcaca tctaccttgc cgatgccagc atcagggaaa
      661 acatcgcgtt cggcgtggcg ccgaccgaca tcgacatggc ggcggtggaa cgcgcggcgc
      721 gcgcggcgca gattcacgat ttcatcgccg gcgacctgga acgcggctac gccacccgca
      781 tcggcgatcg cggcatccgc ctctcgggcg ggcagcgcca gcgcgtcggc atcgcgcgtg
      841 ccctgtaccg cgatccgccg gtactgttct tcgacgaggc caccagtgcg ctcgacacgc
      901 agaccgaaga ggccgtgaac gacgccatcc gttcgctgtc cggcgccaag accatcgtcg
      961 tgatcgcgca caaggaaacc tcgctgcgcg attgcgcgcg cgtcgtgacc ctggcgcccg
     1021 caccgcgcgc tgccgccgcg gcgccgtagt ccatggccaa cgcccgcttc cccaacctgt
     1081 tcatcgtggg tgcgcccaag tgcggcacca cggcgcttag ccattacctt gccggccatc
     1141 cgatgatttt tatgtcggaa caatcagggg cgaaggagcc aaactattat tgtcccgatc
     1201 tcaaccatcc gctggtcatg tttccatatt gcatacgatc aatcgagggc tatttaga
//
LOCUS       DASRVO010000631         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_305069, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000631 DASRVO010000000
VERSION     DASRVO010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305069"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1027
                     /locus_tag="VFF05_07800"
     CDS             77..1027
                     /locus_tag="VFF05_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZW51748.1"
                     /translation="MRNVSPDFSDIETAARRIAPHAHVTPVLRSRSLDELAGCTIAFK
                     CENFQRVGAFKFRGACNAVWALSDAEAAHGVVTHSSGNHGAALALAAKTRGIAAHVVV
                     PEGAVATKVAAIRAYGATVHVCAPTLDARDEAAARIARETGAMLVHPFTNENVIAGQG
                     TAALELLRKTGELDALIAPIGGGGLIGGSAIAAKKIFPQIHVFAAEPEGAAEAFESLR
                     RGVRVTDVEPDTICDGLRATFGEINFTLLRRYGVEVLPVSDAQTIAAMRLVFERLKIV
                     IEPSSAVVLAALLRHRERFANLRVGAIFSGGNVDLHHLPW"
     gene            complement(987..1901)
                     /locus_tag="VFF05_07805"
     CDS             complement(987..1901)
                     /locus_tag="VFF05_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZW51749.1"
                     /translation="MSTDAARRSALIALGILSLVWGFNWIVMKVVLAYVGPFTFSAMR
                     YVFGTAMLFAVLALRGENLASPPWRDTLLIGLAQTMGFQLLVQLALITGGAGKTALLA
                     YTMPFWVIPLAWALLGDRPSARQWLYITIAAAGLVLVLEPWDVHVGMVSALLALAGGA
                     CWAIGTVLAKRLFLATPVSPLRLTAWQMLYGTGFLVVLALCVHERTTVWSPTLIGALV
                     YNGVLSSGLGWAAWLFVVQRLPTNVAGLASLITPLVGIGLAWALLGERPDASESIGIL
                     VIGTALFGVLQPRATVTTADGASPRSRR"
     gene            1963..>2479
                     /locus_tag="VFF05_07810"
     CDS             1963..>2479
                     /locus_tag="VFF05_07810"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HZW51750.1"
                     /translation="MPKQRLRCHWASGDDAAMRDYHDNEWGVPLHDDTRLFEFLVLEG
                     AQAGLSWSTVLNKRAAYRRAFHGFDIDRVARMTDAALDKLLQNPALIRNRMKIHSARN
                     NARAALRVIAEIGSLDAYFWSFVDGKPIRNRWRARGDVPARTELSDRLSKDMKQRGFG
                     FVGSTIVYAHMQ"
BASE COUNT          458 a          812 c          825 g          384 t
ORIGIN      
        1 ccgcactcgt gttcgccgcg ttgtgggtga ccgcctacgc gacgcggcat ttgcgcggat
       61 atgtgccgca ggcggcatcc gaaacgtgag cccggatttt tccgacatcg aaaccgcggc
      121 acggcggatc gcgccgcacg cgcatgtgac gccggtattg cgctcacgct cgctggacga
      181 actcgccggc tgcacgatcg cgttcaagtg cgaaaacttc cagcgcgtcg gcgcgttcaa
      241 gttccgcggt gcctgcaatg cggtgtgggc cttgtccgac gcggaggccg cgcacggcgt
      301 ggtcacgcat tcctcgggta accatggcgc ggcgctggcg ctggcggcaa aaacgcgcgg
      361 catcgcggcg catgtcgtgg tgccggaagg cgcggtggcg accaaggtcg ccgcgatccg
      421 tgcctatggc gccacggtgc acgtctgcgc gcccacgctc gatgcgcgcg atgaggccgc
      481 cgcgcgcatc gcacgcgaaa ctggtgcgat gctggtgcat ccgttcacca acgaaaacgt
      541 gatcgccgga caaggcacgg ccgcgctgga actgctgcgc aaaaccggcg aactggatgc
      601 gctgatcgcg ccgatcggtg gcggtggcct gatcggcggc agcgcgattg cggcaaagaa
      661 gatttttcca caaatccatg tttttgccgc cgaacccgaa ggcgcggccg aagcgttcga
      721 gtcgctgcgc cgcggcgtgc gcgtgaccga tgtcgaaccg gacacgatct gcgatggctt
      781 gcgcgccacg ttcggcgaga tcaatttcac tttgcttcgc cggtacggcg tcgaggtctt
      841 gccggtaagc gatgcgcaaa cgattgccgc gatgcgcctt gtgttcgagc gcctgaaaat
      901 cgtgatcgaa ccttcatcgg ccgtggtgct ggccgcgctc ctgcgtcatc gcgaacgctt
      961 tgcgaacctg cgcgtcggtg cgattttcag cggcgggaac gtggacttgc accatctgcc
     1021 gtggtgacag tggcgcgtgg ctgcaacaca ccgaacaggg ccgtgccgat cacgaggatg
     1081 ccgatggatt cgcttgcgtc cggacgctcg ccgagcaacg cccaggccag gccaatgccg
     1141 accagcggtg tgatcaggct ggccaggccc gccacattcg tcggcaggcg ttgcacgacg
     1201 aacaaccacg ccgcccagcc cagcccggac gaaagcacgc cgttgtagac cagcgcgccg
     1261 atcaatgtcg gcgaccatac cgtcgtgcgc tcgtgcacgc acagcgcgag cacgacgagg
     1321 aagcccgtgc cgtacagcat ctgccaggcg gtcaggcgca gcggcgatac cggcgtggca
     1381 aggaacaagc gcttggccag cacggtgccg atcgcccagc atgcaccgcc ggcgagcgcg
     1441 agcagagcgc tgaccatgcc gacatgcacg tcccacggtt cgagcaccag caccagtccc
     1501 gccgcggcga tggtgatgta cagccactgc cgcgcgctcg gccggtcgcc aagcaacgcc
     1561 caggccagtg gaatcaccca gaacggcatg gtgtaggcga gcaatgcggt cttaccggcg
     1621 ccacccgtga tcaaggcaag ctgcaccagc agttggaatc ccatcgtctg tgcgaggccg
     1681 atcagcaagg tgtcgcgcca cggcggggac gccagattct cgccgcgcaa cgcgagcacc
     1741 gcgaacaaca tcgcggtgcc gaagacatag cgcatcgccg agaacgtgaa cgggccgaca
     1801 taggcgagca ccaccttcat cacgatccag ttgaaacccc agaccaggct caggatgccc
     1861 agcgcgatca gggcgctgcg gcgggcggcg tcggtgctca tgaaattccg gatgcggcgg
     1921 gttgcgtatg atacgcacgc ttgtgcgaca aggaggaatg aaatgccgaa acaacgcctg
     1981 cgttgtcatt gggccagcgg cgacgatgct gcgatgcgtg attatcacga taacgaatgg
     2041 ggcgtgccgc tgcacgacga cacgcgcctg ttcgagtttc tcgtgctcga aggcgcgcag
     2101 gccggactga gctggagcac ggtgctgaac aagcgcgccg cctatcgccg ggcgttccac
     2161 ggcttcgaca tcgaccgcgt cgcgcgcatg accgacgcgg cgctcgacaa gctgctgcag
     2221 aatccggcgc tgatccgcaa ccggatgaag atccattcgg cgcgcaacaa cgcccgcgcc
     2281 gcgctgcgcg tgatcgccga gatcggcagc ctcgacgcgt acttctggtc gttcgtcgac
     2341 ggcaagccga tccgcaaccg ctggcgcgcg cgcggcgacg tgccggcgcg caccgaattg
     2401 tcggaccggc tcagcaagga catgaagcag cgcggcttcg gcttcgtcgg ctcgacgatc
     2461 gtctacgcgc acatgcagg
//
LOCUS       DASRVO010000632         1250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_70662, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000632 DASRVO010000000
VERSION     DASRVO010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70662"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VFF05_07815"
     CDS             complement(<1..1225)
                     /locus_tag="VFF05_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW51751.1"
                     /translation="MLRKKPGFTAVAVLTLALGIGATTALFSVVNSVLLRPLAYPDSQ
                     RLYVIHEVIPQWANSSPLLDANLPDFLIWRRQAQSFEDIALAEGGSMILSGAGEPQQV
                     RITRGSANFLAMLGARLAHGRLFQAEEDTTGHGYSAILSDSIWRSHFNGDPAIVGRSI
                     TLDGIPYTVVGVLPAAFRMPGGLNGFSRTTQVFVPLNGAKSYEQDLIGEFDFTAVGRL
                     RAGITAAQALAELNTIQAAIAQEAHTKLDLRADLRPLQAEMVGSARRGLLLLLAAVGG
                     VLLMVCVNLANLLLARVPGRLRDAGVRKALGATRTRLFRQTLVESLLLAFLAGALGVA
                     TAYFAVRAFALFGPADIPRLSEVRIDALALAFAIAACALTAVLIGTVPAWLVSRANLN
                     ATLGSAGKNTTEGRGA"
BASE COUNT          238 a          388 c          429 g          195 t
ORIGIN      
        1 gcgctccccg cccttcggtg gtgttcttgc cggccgagcc cagggttgca ttcaggttgg
       61 cgcgcgacac cagccatgcc ggtacggtcc cgatcagcac ggccgtcaag gcgcaggcgg
      121 cgatggcgaa ggcgagcgcg agtgcgtcga tgcgaacttc gctcaagcgc ggaatgtcgg
      181 cgggcccgaa aagtgcgaag gcgcgcacag cgaaatacgc cgtcgcgacg cccaatgcgc
      241 cggcaagaaa ggcgagcagc aggctctcga ccagggtctg ccggaagagc cgcgtgcggg
      301 tggcgccgag tgccttgcgc acgccggcat cgcgcaaccg gccgggaacg cgggcgagca
      361 gcaggttggc taggttcacg cacaccatca acagcacgcc gcccacggcc gccagcagta
      421 gcagcaagcc gcgccgcgcg gagccgacca tctccgcctg aagtggacga aggtcggcgc
      481 gcagatccag cttcgtgtga gcttcctggg cgatggcggc ttgaatggta ttcaattccg
      541 ccaaagcctg cgccgccgtg atcccggccc gaaggcgtcc tacggctgtg aaatcgaatt
      601 ccccgatcag gtcctgctcg taggattttg cgccgttgag cggcacaaag acctgcgtcg
      661 tgcgggagaa tccgttcagg ccgccgggca tgcgaaatgc ggcaggcagc acgcccacta
      721 ccgtgtacgg gatgccgtcg agtgtgatgg agcgccccac gatggccgga tcgccattga
      781 aatggctgcg ccagatcgaa tcgctcagga tggcggaata gccatgtccg gtggtgtctt
      841 cttccgcttg gaataatcga ccgtgcgcga ggcgcgcacc gagcatggca agaaaattcg
      901 ccgatccgcg cgtgatacgc acctgttggg gctcgcctgc gccgctcagg atcatggatc
      961 cgccctcggc aagggcaatg tcttcgaacg attgcgcctg ccggcgccag atgagaaagt
     1021 ccgggagatt ggcgtccagc agcggcgatg agttcgccca ttgtggaatg acttcgtgaa
     1081 tcacgtagag ccgctgcgag tcgggatagg cgagcgggcg cagcaacacg ctgttgacga
     1141 ccgagaacag ggcggtggta gcgccgatgc ccagcgccag cgtaagcacg gccacggccg
     1201 tgaagccggg tttcttgcgc agcatgcgaa ccgaatcgcg agcgtcttgc
//
LOCUS       DASRVO010000633         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_200897, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000633 DASRVO010000000
VERSION     DASRVO010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200897"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VFF05_07820"
     CDS             <1..366
                     /locus_tag="VFF05_07820"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZW51752.1"
                     /translation="ASGLQYEVVKEGKGAKPVKTDTVTVNYVGTKIDGTEFDSSIKRG
                     QPAKFPLANVIPGWTEGLQLMPVGSEYKFFIPAKLAYGEHGPPQIGPDATLIFDVTLI
                     SIDKPEAAKPAAPAAPKKD"
     gene            470..1822
                     /locus_tag="VFF05_07825"
     CDS             470..1822
                     /locus_tag="VFF05_07825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZW51753.1"
                     /translation="MRITLYGTGYVGLVTGTCLAEVGNDVVCVDVDAAKIADLERGKM
                     PIYEPGLAELVESNRAAGRLKFTTDAAQGIAHGQMLFIAVGTPPDEDGSADLSHVLTV
                     ARTIGAHIDGYRVVVNKSTVPVGTADRVREAIAVELARRGVSHEFDVVSNPEFLKEGD
                     AVKDCMRPDRIVIGSHSARAIESLKALYAPFNRNHERIVLMDTRSAELTKYAANAMLA
                     TKISFMNEMANIAERVGADIELVRHGIGSDPRIGYSFIYPGAGYGGSCFPKDVQALER
                     IARSHGYEARVLDAVETVNRNQKEKLFELLSRHFGGNLSAKTIALWGLAFKPNTDDMR
                     EAPSRRLLEQLWSAGAKVRAFDPEARDEAQRLYGKRVDLVLCDEMYAALDGADALIVV
                     TEWKAFWSPDFTRIKSALRTPVVFDGRNIYEPAAAEAAGIAYYGIGRGRSLRDVAGRS
                     "
     gene            1819..>2305
                     /locus_tag="VFF05_07830"
     CDS             1819..>2305
                     /locus_tag="VFF05_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HZW51754.1"
                     /translation="MSFSLHIQDARIGVIGLGYVGLPLAVALGREFPTLGYDRNSARV
                     AELQAGHDATGEMDAAELASAKKLRYGADAAALKDCNVYIVAVPTPVTPHKMPDLSPL
                     VSASETIAAALKHGDVVIYESTVYPGATEEVCVPVLERFSGKKFNAGFFCGYSPERIN
                     PG"
BASE COUNT          446 a          719 c          750 g          390 t
ORIGIN      
        1 gcctccggac tgcagtacga agtggtcaag gaaggcaagg gcgccaagcc ggtcaagacc
       61 gacaccgtca ccgtgaatta cgtcggcacc aagatcgacg gcaccgagtt cgacagctcg
      121 atcaagcgcg gccagccggc caagttcccg ctcgcgaacg tgattccggg ctggaccgaa
      181 ggcctgcaac tgatgccggt gggttcggaa tacaagttct tcattccggc caagctggct
      241 tatggcgagc acggcccgcc gcagatcggc ccggacgcga cgctgatctt cgacgtcacc
      301 ctgatcagca tcgacaagcc tgaagcggcc aagccggccg ctccggcagc gccgaagaaa
      361 gactgagcgg gtttcacgca ggtgacgaaa aggcgcgggc aaccgcgcct ttttcgttgg
      421 tgcggcttgg tacaatgcgc gttcttacaa gcgcgccggg aaaaatgcga tgcggataac
      481 cctgtacggc accggttacg tgggcctggt gaccgggact tgtctcgccg aagtcggcaa
      541 tgatgtcgtc tgcgtggacg tggacgcggc caagatcgcg gacctggaac gcggcaagat
      601 gccgatttac gagccgggac tggcagaact ggtcgagtcc aaccgcgccg ccggacgcct
      661 gaaattcacc accgatgccg cgcagggcat cgcccacggc cagatgttgt tcatcgccgt
      721 cggcacgccg ccggacgagg acggcagcgc cgacttgtcg catgtgctca ccgtcgcacg
      781 cacgatcggc gcacacatcg acggttatcg ggtggtcgtc aacaaatcga ccgtgcccgt
      841 gggaactgcc gatcgcgtcc gcgaggcgat cgccgtggaa ctcgcgcgac gcggcgtcag
      901 ccacgaattc gatgtcgtct ccaatccgga attcctgaag gaaggcgacg cggtcaagga
      961 ctgcatgcgc ccggaccgca tcgtcatcgg ttcgcacagc gcacgagcga tcgaatcgct
     1021 gaaggcgctg tatgcgccgt tcaaccgcaa tcacgaacgc atcgtgctga tggacacgcg
     1081 ttcggcggaa ctcaccaaat acgcggccaa cgcgatgctg gcgacgaaga tcagtttcat
     1141 gaacgagatg gcgaacatcg ccgagcgcgt cggtgccgac atcgaactcg tgcgccacgg
     1201 catcggttcg gatccgcgca tcggttattc cttcatctat cccggcgccg gctatggcgg
     1261 ttcgtgtttc cccaaggacg tgcaggcgtt ggagcgcatc gcgcgcagcc acggctacga
     1321 ggcgcgcgtg ctcgatgcgg tggaaaccgt caatcgcaac cagaaggaaa agctcttcga
     1381 gctgttgagc cggcactttg gcggaaacct ttccgccaag accattgcgt tgtggggact
     1441 tgctttcaag ccgaataccg acgacatgcg cgaagcgccg agccggcgcc tgctcgaaca
     1501 gctctggtcc gccggtgcga aggtgcgtgc gttcgatccg gaagcgcgcg acgaagcgca
     1561 gcgcctctac ggcaagcgtg tcgacctggt gctgtgcgat gagatgtacg cggcgctcga
     1621 cggcgccgac gcgctgatcg tggtcaccga atggaaggca ttctggagtc cggatttcac
     1681 gcgcatcaaa tccgccttgc gcacgcccgt cgtgttcgac ggacgcaaca tctacgagcc
     1741 ggctgccgcc gaagccgcgg gcatcgccta ttacggcatc ggccgcggcc gcagcctgcg
     1801 cgacgtggcc gggcgttcat gagtttttcg ctgcatatcc aggatgcgcg catcggcgtg
     1861 atcgggctgg gttacgtcgg cctgccgctg gctgtcgcgc ttggacgcga atttccgacc
     1921 ctgggctacg accgcaattc cgcgcgcgtg gccgagcttc aggccggaca cgatgccacg
     1981 ggcgagatgg acgcggcgga actggcgtcc gcgaagaagc tgcgctacgg cgcggacgcg
     2041 gcggcattga aagactgcaa tgtttacatc gtcgccgtac cgacgccggt gacgccgcac
     2101 aagatgccgg acttgtcgcc gctcgtgtcc gcgagcgaaa ccatagccgc ggcgctcaag
     2161 cacggcgatg tggtgatcta cgaatccacc gtgtatccgg gcgcgacgga agaagtgtgc
     2221 gtgcccgtgc tcgagagatt cagcggcaag aagttcaatg ctggcttctt ctgcggctac
     2281 agtcccgagc gaatcaatcc cgggg
//
LOCUS       DASRVO010000634         1349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_330527, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000634 DASRVO010000000
VERSION     DASRVO010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330527"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFF05_07835"
     CDS             <1..616
                     /locus_tag="VFF05_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51755.1"
                     /translation="TPPPSLPPPPAAPTPADLAVYPGLYRIAAGSDFVVRIGADGLIA
                     QFPGQFPVELRAVGHDVFAATGDAFVLSFQREGDAVVSILLGHGGTNLLAQRLSERAP
                     ALARTALASNGKKLLEYRGDYRLDADTLLRIAARDGTLSVQPTGHAALTLTAFAPDRF
                     ADPRGACEIHFLRNDKHEVTSLKIVLAGAERSAERSAWHVPALR"
     gene            649..>1349
                     /locus_tag="VFF05_07840"
     CDS             649..>1349
                     /locus_tag="VFF05_07840"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017914585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HZW51756.1"
                     /translation="MTALSVNVNKIAVLRNSRGGNEPDVRRAARTCIAAGCQGITVHP
                     RPDRRHIHAEDVRALAALCTGGIEFNIEGNGFAPPRQGYPGLLALVRETRPAQCTLVP
                     DGDGQITSDHGFDLRADATRLRPLIAELKSCGARVSLFMDAGTGDIARAAELGADRVE
                     LYTGPYAAAFARGEQARELDACVASAEAARAAGLGVNAGHDLDQANLPMLKAAIPYLA
                     EVSIGHALISEALYAG"
BASE COUNT          214 a          473 c          436 g          226 t
ORIGIN      
        1 tacgccaccg ccatcgctgc caccgccgcc ggcagcgcca acgcccgcgg acttggccgt
       61 ctatcccggt ttgtatcgaa tcgccgcagg cagcgatttc gtcgtgcgca tcggcgccga
      121 tggcttgatc gcgcagttcc ccgggcaatt tccggtcgag ctgcgcgccg tcggtcacga
      181 cgtcttcgcc gccacgggcg atgcgttcgt gctttcgttc cagcgtgaag gcgatgccgt
      241 cgtatccatc ctgctcggac acggcggcac gaacttgctt gcgcaacgcc tgagcgaacg
      301 ggcgccggca ctcgcacgca cggcgcttgc cagcaacggc aagaagcttc ttgaatacag
      361 gggcgattat cgcctcgatg ccgacacctt gctgcgcatc gccgcgcgcg acggcacgct
      421 gagcgtgcag ccgaccggac atgcagcgct cacgctgacc gcattcgcac cggatcgctt
      481 cgccgatccg cgcggtgcct gcgaaatcca tttcctgcgg aacgacaagc acgaggtcac
      541 ttcgttgaag atcgtgctcg ccggcgccga gcgcagcgcc gagcgcagcg cctggcatgt
      601 gccggcgctg cgctagcgcg cgccattgcg cttgcgtatc cttttgtcat gaccgcactc
      661 agcgtcaacg tcaacaagat cgccgtgttg cgcaactcgc gtggcggcaa tgagccagac
      721 gtgcgccgtg ccgcgcgaac ctgcatcgcc gccggctgcc agggcatcac cgtgcatccg
      781 cggccggacc ggcggcatat ccacgccgaa gacgttcgcg cgctcgccgc actgtgcact
      841 ggcggcatcg aattcaacat cgaaggcaac ggtttcgcgc cgccgcgcca aggttatccg
      901 ggtctgctcg cgctggtgcg cgaaacacgg cccgcgcaat gcaccctggt tccggatggc
      961 gacggccaga tcacgtccga tcacggcttc gacttgcgtg ccgatgcgac gcgcctgcgt
     1021 ccgttgatcg cggaactgaa atcctgcggc gcgcgggtaa gcctgttcat ggacgccggc
     1081 accggagaca tcgctcgcgc cgccgagctg ggcgccgatc gcgtggaatt gtatacaggc
     1141 ccctatgcgg ccgcattcgc gcgcggcgag caggcgcgcg aactggatgc ctgcgtcgcg
     1201 tcggccgagg cggcgcgcgc cgccggtctg ggcgtgaatg caggccacga cctggatcag
     1261 gccaatctcc ccatgctcaa ggccgcgatt ccgtatctgg cggaagtctc gatcggccac
     1321 gcgctgatca gcgaggcgct gtacgcggg
//
LOCUS       DASRVO010000635         1354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_305232, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000635 DASRVO010000000
VERSION     DASRVO010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305232"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..>1354
                     /locus_tag="VFF05_07845"
     CDS             36..>1354
                     /locus_tag="VFF05_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51757.1"
                     /translation="MSGSAARCVIDLRNTGPANVWALVDIPQNNPSGNYTVSLDSGIP
                     SVTYRVPGGVRGDFGVVGPGHANAQAAFPLRLFWGSPSGMAALTPNTRYYGAVLIDPL
                     NGGNFAALGQAGLVPFALTRASGSDDVADALEPDATRTLSLAAGETLAHQFIDVPGQA
                     TLLVDTSYASGSSNRGAVNFRLTHADFPASASTPQIAAAPAASSSDAQWSLGGATTSK
                     SVTVPVNAGRWYLVAATAGASGADVALAVTTNLIQPVSPVVAGAYYNPQRSGHGIYMS
                     QTGGSQVAYWYTYLEDGTPIWYAGLNTAPPAGSGAWTAPLARVTWDGSAINAYADVGN
                     VIITPISATDVMYSWHLYGTSGSEHFTLIGTPQCLPFNGSQSDFTGQWYAPAQSGYGM
                     DVFAQTGFQNDTFYFYDSLGQPHWADGNISSFGPSATVTLMQYTGFCP"
BASE COUNT          227 a          442 c          431 g          254 t
ORIGIN      
        1 cgagttacgc cgagcagcaa tgcgccgcat cgagcatgtc gggatcggcc gcgcgttgcg
       61 tgatcgatct gcgcaacacg ggcccggcga atgtctgggc gctggtcgac atcccgcaga
      121 acaatccgtc gggcaactac acggtatcgc tcgatagcgg tatcccctcg gtgacctatc
      181 gggtgccggg cggcgtgcgc ggcgatttcg gcgtggtcgg tcccggccac gccaacgcgc
      241 aagccgcatt tccgttgcgc ctgttctggg gatcgcccag cggcatggct gcgctcacgc
      301 cgaatacgcg ttattacggc gcggtgctga tcgatccgtt gaacggtgga aatttcgctg
      361 cgttgggcca ggccggtctc gtgcccttcg cattgacgcg tgcgtcgggc agcgacgatg
      421 tcgccgatgc gctcgagccg gatgcgacgc gcaccttgtc gctggctgcg ggcgagacgc
      481 tcgcgcacca gttcatcgat gtgcccgggc aggcgacgct gctcgtcgat acgagttacg
      541 ccagcggttc ttcgaatcgc ggcgcggtga atttccgcct gacccacgcc gacttcccgg
      601 cctcggcgtc cacgccgcaa attgccgctg cgcccgccgc gtcatcgagc gacgcgcaat
      661 ggtcactggg cggcgcgacg acgagcaaat ccgtcaccgt gccggtgaat gccggacgct
      721 ggtacctggt cgccgcgacc gccggcgcaa gcggtgccga tgtcgctttg gccgtgacga
      781 cgaacctcat ccagcccgtg tcgcccgtcg ttgccggcgc ctattacaac ccgcagcgtt
      841 cgggccacgg catctacatg agccagactg gcggcagtca ggttgcttat tggtacacgt
      901 acctcgaaga cggcacgccg atctggtacg cgggcctgaa cacggcaccg ccggccggct
      961 ccggcgcatg gacggcaccg ctggcgcgcg taacctggga cggatccgcg atcaatgcct
     1021 acgctgacgt tggcaatgtc atcatcacgc cgatcagcgc cactgacgtg atgtactcat
     1081 ggcatctcta cggaacctcg ggcagcgaac atttcaccct gatcggcacc ccgcaatgct
     1141 tgcctttcaa tgggtcgcag agtgatttca ccgggcaatg gtatgcgccg gcgcaatcgg
     1201 gatacggcat ggacgtgttt gcccaaacgg gctttcagaa cgacacgttt tatttttacg
     1261 atagcctcgg ccaaccgcac tgggccgacg gcaacatttc gagtttcggg ccgagtgcga
     1321 cggtgacgct catgcagtac acgggatttt gccc
//
LOCUS       DASRVO010000636         1475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_381869, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000636 DASRVO010000000
VERSION     DASRVO010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381869"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VFF05_07850"
     CDS             complement(<1..1326)
                     /locus_tag="VFF05_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZW51758.1"
                     /translation="MAAAQANQSSVLIVTTHRDDRRVLFDTLDAQNFEAVYTAKDLPQ
                     ALAFLKQDPQIDVVVMEFLGEAREAVAFCTQLKGDPRLAKVPVIGIVATDAAQRHWDW
                     SRAPAGVVDWLRSPVDASEALARVRNVLAPHAAVGKGEGATGSDRYQFAFEDSLDEIV
                     LSDPKTGAVIDANATFEQRSGFSRAQVIGQALDALDTSHDRNQRAALLATLAREGIVQ
                     YRCNRRRGDGGGHAVEAYMRLAVQDGRLVHVTVFREPSGGSDGMRYKAALGMIAAAAE
                     CGGGDEGMGQVVKLLCGWLDPDFVMLVSARPEEDAEMQPLHTYYRSSLPAEAPDPLKQ
                     PVLKRLLDGESVFHGEKAWQRSEQDPFLQFFQFEAFIGLPLRDERRSTLGALMIARRD
                     ALAADALLIDTLRAIAARLALELELRRAREQGRAKGLQDALTGLPNRLLF"
BASE COUNT          240 a          519 c          470 g          246 t
ORIGIN      
        1 gaacagcagc cggttcggca gtccggtgag cgcatcctgc aagcctttgg cacggccttg
       61 ctcgcgcgcg cgacgcagtt cgagctccag cgcgagacgc gcggcgatcg cacgcagggt
      121 atcgatcagc agtgcgtcgg cggccagcgc atcgcggcgc gcgatcatca gggcacccaa
      181 cgtactccgg cgctcgtcgc gcaagggcaa gccgatgaac gcctcgaact ggaagaattg
      241 caggaacgga tcctgttccg agcgttgcca tgccttttcg ccgtggaaca ccgactcccc
      301 gtcgagcagg cgcttgagga ccggctgttt cagcggatcc ggtgcttcgg ctggcaggct
      361 ggaacgatag tacgtatgca acggctgcat ttcggcgtct tcttccggcc gcgcagacac
      421 cagcatgacg aagtccggat cgagccagcc gcacaggagt ttcacgacct gtcccatgcc
      481 ctcgtcgccg ccgccgcatt cggccgccgc cgcgatcatg ccgagcgccg ccttgtagcg
      541 catcccgtcg ctgccgccgg agggttcgcg gaataccgtt acgtggacca ggcgtccatc
      601 ctgcacggcc aggcgcatgt acgcttcgac cgcgtggccg ccaccgtcgc cgcggcgccg
      661 attgcagcgg tattgcacaa tgccttcccg cgcgagcgtg gcgagcagtg ccgcgcgctg
      721 gttgcggtcg tggctcgtgt cgagcgcatc cagcgcctga ccgatcacct gcgcgcgcga
      781 aaatccggaa cgctgctcga atgtcgcgtt ggcgtcgatc actgcgccgg tcttggggtc
      841 cgacagcacg atttcgtcga gactgtcttc gaaggcgaac tgataacggt cgctgccggt
      901 ggcgccctcg cccttgccga cggccgcatg tggcgccagc acgttgcgta cgcgcgccag
      961 cgcctcgctc gcatccaccg gactgcgcag ccagtcgacc acgccggccg gcgcgcgcga
     1021 ccagtcccag tgccgctgtg ccgcatcggt cgcgacgatg ccgatcaccg ggaccttggc
     1081 caggcgcgga tcgcccttga gctgggtgca gaacgccacc gcctcgcgcg cctcgccgag
     1141 gaattccatc accaccacat cgatctgcgg gtcctgcttg aggaaggcca gcgcctgcgg
     1201 cagatctttc gccgtgtaca ccgcttcgaa attttgcgcg tcgagcgtgt cgaacagcac
     1261 gcggcgatcg tcgcgatgcg tcgtcacgat caataccgaa ctctgattgg cttgcgcagc
     1321 ggccatcaaa cgacccaacg tccgtcgcgc aggcgcggac gcgtgcccgc aaccggctgg
     1381 tgcgagatga cttccaccag agcgtccata ccctcgggca tggcgatctc gtcgccgaca
     1441 aacacgacct tgcgcccggt cacgctgcgc gagcg
//
LOCUS       DASRVO010000637         1070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_330610, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000637 DASRVO010000000
VERSION     DASRVO010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330610"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="dapA"
                     /locus_tag="VFF05_07855"
     CDS             complement(<1..872)
                     /gene="dapA"
                     /locus_tag="VFF05_07855"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZW51759.1"
                     /translation="MHKLTGSICALATPFRGDGLDLAAFGALIDYQLAGGTQGLVVAG
                     STGEAHALSEAEFERALAFALERVAGRVPVLAGTGTASTRKTITATRRAHAIGADAAL
                     VVTPYYVRPTQEGLYRHFSATADEAGLPIVLYNVPSRTGCDMLPETAARLAAHERIAG
                     IKEACNDDARIAAVVALKRPDFAVLSGDDGTCAQAMLSGADGVISVAANVAPAAMRAL
                     CDAALANTAGRVRELDGKLTELYALLGTEPNPIPVKWCLHEIGICLPDPRLPLTPFGT
                     MHHEGARQVLGALNL"
BASE COUNT          189 a          353 c          358 g          170 t
ORIGIN      
        1 aggttcaagg caccgagcac ctgccgcgca ccttcatgat gcatggtacc gaacggcgtc
       61 aacggaaggc gcgggtcagg cagacaaatc ccgatctcgt gcaggcacca ttttaccgga
      121 atcgggttcg gttcggtgcc gagcaaggcg taaagttcgg tgagtttccc gtccagttcc
      181 cgcacacgtc cggcggtgtt ggccaaggcg gcatcgcaga gcgcgcgcat ggcggcgggc
      241 gcaacattgg cggcaaccga aatcacgcca tcggcgccgc tcagcatggc ctgcgcacag
      301 gtaccgtcgt cgccgctgag cacggcgaaa tccgggcgct tgagcgcaac gacggcggca
      361 atgcgtgcgt cgtcgttgca tgcttccttg atgccggcga tgcgctcgtg cgcagccagg
      421 cgtgccgcag tttccggcag catgtcgcag ccggtgcgcg aaggcacgtt gtacaacacg
      481 atcggcaggc ccgcttcgtc ggcggtggcg gaaaagtgcc gatacaaacc ctcttgcgtc
      541 ggccgcacgt agtaaggcgt caccacgagg gcggcatccg caccgatggc atgcgcgcga
      601 cgcgtagcgg ttatggtctt gcgcgtgctc gcggtgccgg taccggcgag caccggcacg
      661 cgcccggcca cgcgttcgag cgcgaacgcg agcgcacgtt cgaattcggc ttcgctcaag
      721 gcgtgcgctt cgccggtgga gccggccacc acgagcccct gcgtgccgcc ggcaagctgg
      781 tagtcgatca gcgcgccgaa cgcggccaga tcgagcccgt cgccgcggaa cggcgtggcc
      841 agcgcacaga tgctgccggt gagtttgtgc aagacgcgtc tcgaatgtcg cgggggccga
      901 gaatgttact tgccacatgt tggagcggca agtattctcg caggcgacgc cggcattcac
      961 cacgacatgc gcaagattcc ggaagtagct gcacccatcg attccgcccc gcgcccggcc
     1021 gcgaacgaaa actatctgct gatcaacgcg ttcgcccccg ccgcgcggcc
//
LOCUS       DASRVO010000638         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_381923, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000638 DASRVO010000000
VERSION     DASRVO010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381923"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VFF05_07860"
     CDS             <1..776
                     /locus_tag="VFF05_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1974 domain-containing protein"
                     /protein_id="HZW51760.1"
                     /translation="LLRNATRAKLLAWTGSRLAAAPRTEDTVRYYQHLSRFSAAFELL
                     SDVAMGTLGGTLKRREKISGRLADALAYMYLASAALKRYHDEAKTTANYNLVRWSVEL
                     CLYRIQEALLGVLENLPMRAAATWLSWVIFPLGPRFRPPSDKLGTRVARDILEDREAR
                     INLTQDIFVPPANEVGLGALEAALDKAVRAIPVETKLRDAVRAGKLDRAPGYMLDEMG
                     LKAGVISQAEYDLIQEADAARNEVVAVDAFDPEVYKTLH"
     gene            782..1432
                     /locus_tag="VFF05_07865"
     CDS             782..1432
                     /locus_tag="VFF05_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HZW51761.1"
                     /translation="MAAPTAHLFVAALLAFVAEASATQVYKCTDAQGNDAFQDRPCPQ
                     NAQQAEVWLPDLAPAPAQPADAPGASSAASVLPPPVPISKPPAPLPPLWICRNAEDGS
                     QYFSRSGTPPVRYVPLGVLGYPGHSLAQAYGPGGIGVSAPGMRQIPIDHSPRDAIASQ
                     YTPLQDECAHASREQTCGWLQKQYDDVMHKLHNAFKDQQAILQPQADELQNQLNGC"
     gene            complement(1433..1933)
                     /locus_tag="VFF05_07870"
     CDS             complement(1433..1933)
                     /locus_tag="VFF05_07870"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZW51762.1"
                     /translation="MIDVYYDYLDSPVGRLLLVADGQGLRHVAFERGRHPTPVADDWE
                     RGPGALHETRAQLKAYFAGKLREFDLPLAPQGTDFQQRVWLALLRIPYGETASYGDIA
                     RAVGEPGASRAVGAANGQNPIAIVVPCHRVIGANGTLTGYGGGLPNKKFLLDLEQKNS
                     AFALAP"
     gene            complement(1946..3400)
                     /locus_tag="VFF05_07875"
     CDS             complement(1946..3400)
                     /locus_tag="VFF05_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HZW51763.1"
                     /translation="MEPSRVGLDPKACEQARLTRDARFDGLFFTAVRTTGIYCRPVCP
                     APTAHARNVEYFPNAAAAASAGYRPCLRCRPEAAPGTPLTRARSELVAAALRMIEAGA
                     PEAASVARLAHRIGVGERHLRRLFVAELGAGPLEIAATRRLLFAKKLLTETALPVTQV
                     AHAAGYASLRRFNAAFLDGYGRAPRVIRREQSAPANNTLTLRVPFRAPYDFSALLAFF
                     ERRAIPGLEQVDANSYTRRFAFDGKAGSLRVTRMRGTDALGLTIDFPDTAQLQAICAR
                     VRRLLDVDADIAAITAQLARDPRLRGFVRRHPGQRLPGGWDGFEIAVRAVLGQQVSVA
                     AARTLATRLLERFGEPATLPNGEAFRLFPSPSTLADADLTRIGLTGARAKTLNAIARA
                     LCDGGVDFRPEQSLDNFVASWCELPGIGAWTAHYIAMRALGHPDAFPAADLVLRKMLA
                     DGGTALSARAVEALAERWRPWRAYAVMHLWRNSN"
BASE COUNT          610 a         1190 c         1117 g          525 t
ORIGIN      
        1 tgctgttgcg caatgccacg cgcgcgaagc tgctggcctg gaccggcagc cgcctcgccg
       61 ccgccccgcg caccgaggac accgtgcgct attaccagca cctgtcgcgt ttttccgccg
      121 cgttcgaact gctttcggac gtcgccatgg gcacgctcgg cggcacgctc aagcgccgcg
      181 agaagatttc cggacgcctc gccgacgcgc tcgcctacat gtatctggct tccgccgcgc
      241 tcaagcgcta tcacgacgaa gcgaaaacga ccgcgaacta caacctcgtg cgctggagcg
      301 tggagctgtg cctgtaccgc atccaggaag cgctgctcgg ggttctggaa aacctgccga
      361 tgcgcgccgc ggcaacgtgg ttgtcgtggg tcatcttccc gctcggcccg cgcttccgcc
      421 cgccctcgga caagctcggc acgcgcgtcg cgcgcgacat cctcgaagac cgcgaagcgc
      481 gcatcaacct gacgcaagat atcttcgtgc cgccagccaa cgaggtcggc ctcggcgcgc
      541 tggaagccgc gctcgacaag gccgtgcgcg cgatcccggt ggaaacgaaa ctgcgcgacg
      601 ccgtgcgtgc cggcaagctc gaccgcgcgc ccggctacat gctcgacgag atgggcctga
      661 aagccggcgt gatctcgcag gccgaatacg acctgatcca ggaagccgat gccgcacgca
      721 acgaagtcgt cgccgtcgac gcgttcgatc cggaggttta caagacgttg cattgagcgc
      781 catggctgca ccgactgccc acctttttgt cgccgcattg ctggctttcg tcgcggaagc
      841 ctccgcgaca caggtataca aatgcaccga cgcgcagggc aatgacgcgt tccaggatcg
      901 gccgtgtcca caaaatgcgc aacaggcgga agtctggctg ccggatttgg cgcccgcgcc
      961 ggcgcagcca gccgacgcac cgggcgcgtc atcggcagcg tccgtcttgc cgccgccggt
     1021 gccgatcagc aagccgcctg cacccttgcc gccgttgtgg atctgccgca atgccgagga
     1081 cggctcgcaa tacttcagcc ggagcggtac accgccggta cgctatgtgc cgctcggcgt
     1141 gctcggctac ccggggcaca gtttggcgca ggcttacgga cccggcggca tcggcgtgtc
     1201 ggcgccggga atgcgccaga ttccgatcga ccattcgccg cgcgatgcga tcgccagcca
     1261 gtacacgccg ttgcaggacg aatgcgcgca cgcctcgcgc gaacaaacct gcggctggct
     1321 gcaaaaacag tacgacgacg tgatgcacaa gctgcacaac gcgttcaagg accaacaggc
     1381 gatcctgcaa ccgcaggcgg atgaactgca aaaccagctc aatggttgtt gatcacggcg
     1441 ccagcgcgaa tgccgaattt ttctgctcga ggtccagcag gaatttcttg ttcggcaagc
     1501 cgccaccgta gccggtgagc gtgccgttcg caccgatcac gcgatggcat ggcaccacga
     1561 tggcaatcgg attctgcccg ttcgccgcgc cgaccgcgcg cgaggcgccg ggctcgccga
     1621 ccgcacgcgc gatgtcgccg taacttgccg tttcaccgta gggaatccgc agcaacgcca
     1681 gccatacgcg ctgctggaaa tccgtgcctt gaggcgcgag cggcaggtcg aactcgcgca
     1741 gctttccggc gaaatacgcc ttcaattgcg cgcgcgtttc gtgcagcgcg ccggggccgc
     1801 gctcccaatc gtccgcgacc ggcgtcggat ggcggccgcg ttcgaacgcg acatggcgca
     1861 ggccttgtcc atccgccacg agcagcaggc gtccgaccgg ggaatcgaga tagtcgtaat
     1921 acacgtcgat cattgtacga gtccttcagt tcgagttgcg ccacaaatgc atgaccgcgt
     1981 aggcgcgcca cggccgccag cgttccgcga gcgcttcgac tgcgcgtgcg gacagcgcag
     2041 tgccaccgtc ggccagcatc ttgcgcagca ccagatccgc agccgggaat gcgtccggat
     2101 ggcccaacgc gcgcatcgcg atgtaatgcg cggtccatgc gccgatgccg ggcagctcgc
     2161 accagctcgc gacgaaatta tccagcgatt gctcgggccg gaaatccacg ccgccgtcac
     2221 acaacgcgcg tgcgatcgcg ttcaacgttt tcgcacgcgc gccggtcaag ccaatgcggg
     2281 tcaggtcggc gtcggccagc gtcgacggcg atggaaacaa acgaaacgct tcgccattcg
     2341 gcaaggtcgc cggttcgcca aagcgttcga gaagccgtgt cgcaagcgtg cgcgctgccg
     2401 ctacgctgac ctgctgaccg agcaccgcgc gcaccgcgat ctcgaaaccg tcccagccgc
     2461 cgggcaggcg ttgacccgga tgacggcgca cgaacccgcg caggcgagga tcgcgcgcga
     2521 gttgtgcggt tatcgcggcg atgtccgcat ccacgtcgag caagcggcgc acgcgtgcgc
     2581 aaatcgcttg cagctgcgcc gtgtccggga aatcgatcgt cagcccgagc gcgtccgtgc
     2641 cgcgcatccg cgtcacgcgc agcgatccgg ccttgccgtc gaacgcgaag cgtcgcgtgt
     2701 aactgttcgc gtcgacctgt tcgagcccgg gaatcgcgcg gcgctcgaag aacgcaagga
     2761 gcgcggaaaa atcgtagggc gcacgaaacg gcacgcgcaa cgtcaacgtg ttgttcgccg
     2821 gtgcggactg ttcacgtcgg atgacgcgcg gcgcacgccc atagccgtcg aggaaggcgg
     2881 cattgaaccg gcgcaggctc gcgtaaccgg ccgcatgcgc gacctgcgtc accggcaacg
     2941 cggtttcggt cagcagtttc ttcgcgaaca gcaggcgccg cgtcgccgcg atttccagcg
     3001 gcccggcgcc aagttcggcg acgaacaggc ggcgcagatg acgctcgccg acgccgatgc
     3061 ggtgcgcgag tcgcgcgacg gacgccgcct ccggcgcgcc ggcctcgatc atgcgcaacg
     3121 ccgcggcgac gagttcgctg cgcgcgcgcg tgagcggcgt gcccggcgcc gcttccggcc
     3181 ggcagcgcag gcacggccgg taaccggcgc tcgccgcagc cgccgcgttc ggaaaatatt
     3241 cgacattgcg cgcgtgtgca gtcggcgccg ggcacaccgg gcggcaatag atgccggtcg
     3301 tacgcacggc ggtgaagaac aggccgtcga aacgcgcatc gcgcgtcagg cgcgcctgtt
     3361 cgcaggcttt cggatcgagt ccaacgcggg aaggttccat cgacatggcg gcgatcctaa
     3421 cgccgccgct gtctcgaaac ta
//
LOCUS       DASRVO010000639         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_122865, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000639 DASRVO010000000
VERSION     DASRVO010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122865"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1049
                     /gene="glnS"
                     /locus_tag="VFF05_07880"
     CDS             <1..>1049
                     /gene="glnS"
                     /locus_tag="VFF05_07880"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HZW51764.1"
                     /translation="LTEPGRNSPFRDRPVAENLDLFRRMKAGEFPNGARVLRARIDMA
                     SGNINLRDPVLYRILHATHPRTGDKWSIYPSYDYAHGQSDAIEGVTHSLCTLEFEDHR
                     PLYEWFLDNLPVPSRPRQYEFARLNLNYTVMSKRFLAQLVREGRVAGWDDPRMPTLAG
                     LRRRGVPAAALREFVKMAGVARANSVVDIQMYEHAVRDYLNRAAQRRMAVLRPLKVVI
                     ENYPEGKSEELEAINHPDDPAAGTRRIRFGREIFVEREDFMENPPKKFFRLSPGKEVR
                     LRYAYFITCREVKKNAAGEVTELRCTYDPVTKGGNAPDGRKVKSTMHWVSGGHAAEAS
                     VALYERLFSAAYPGE"
BASE COUNT          186 a          342 c          346 g          175 t
ORIGIN      
        1 cctgaccgag cccggccgga acagcccgtt ccgcgaccgt ccggttgcgg aaaacctcga
       61 tctgtttcgc cgcatgaaag ccggcgagtt tccgaacggc gcccgggtgc tgcgcgccag
      121 gatcgacatg gcttccggca acatcaacct gcgcgacccc gtgctgtatc gcatcctgca
      181 cgccacccat ccgcgcaccg gcgacaaatg gtcgatctac ccgagctatg actatgccca
      241 tggccagtcg gacgcgatcg agggcgtgac gcattcgctg tgcacgctcg aattcgaaga
      301 tcaccggccg ctctatgaat ggttcctcga caatctgccg gtgccgtcgc ggccgcggca
      361 atacgagttc gcgcggctca atctcaacta cacggtgatg tcgaagcgtt tcctcgcgca
      421 gctcgtgcgc gaggggcggg tcgcgggctg ggacgatccg cgcatgccga ccttggcggg
      481 cttgcgccgg cgcggcgtgc ccgccgcggc tttgcgcgaa ttcgtcaaga tggcgggcgt
      541 cgcccgcgcc aacagcgtcg tcgatatcca gatgtacgag cacgcggtgc gcgattattt
      601 gaaccgcgcg gcgcagcgcc gcatggccgt gctgcggccc ttgaaggtgg tgatcgagaa
      661 ctatcccgag ggcaagagcg aagagctcga ggcgatcaac catcccgacg acccggccgc
      721 cggcacgcgg cgcattcgct tcgggcgcga gattttcgtc gagcgcgagg atttcatgga
      781 gaacccgccc aagaaattct tccgcctctc gcctgggaaa gaagtccggc tgcgctatgc
      841 ctatttcatc acctgccgcg aggtgaagaa gaacgccgct ggcgaggtca cggaattgcg
      901 ctgcacctac gatccggtga ccaagggcgg caacgcgccc gacggacgca aggtgaagtc
      961 gacgatgcac tgggtctcgg ggggccatgc cgctgaggcg agcgtggcac tgtacgagcg
     1021 gctgttctcg gcggcgtacc ccggggagc
//
LOCUS       DASRVO010000640         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_18750, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000640 DASRVO010000000
VERSION     DASRVO010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18750"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VFF05_07885"
     CDS             <1..1092
                     /locus_tag="VFF05_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51765.1"
                     /translation="VLALPAFARRPRLFVIAAAAFAAIPYDWENIGMGFANSYFLLAG
                     FSVFTIVSAARVRRIVPGMIALLAFALLADVSMGSGFFAAAIGLCIVLARWHRGDLGT
                     HAVLAFATTLAACAVCGVVLTWHATGHASIGALQALQVMLAVFVWIPTAVLGYCCLRS
                     HTVDRCDLALLGVSLWGFAEILAIIAMRPEIRLWFPISRYMEILGIAAFANIGCLMRA
                     AQSMPALRRLARWLLPAFACAIVLGTPFAAYWFDWRANNLAAQAQRVVRYVKRGDAAA
                     WTAAPVTELPFPSPDYLRAELDADDVRYILGDVYGTRPQPALLTAFWRGFGADLRAWR
                     FAIWPAAALLAWLLLPPAWRRMRYATIPQ"
     gene            1089..1820
                     /gene="fabG"
                     /locus_tag="VFF05_07890"
     CDS             1089..1820
                     /gene="fabG"
                     /locus_tag="VFF05_07890"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008571168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZW51766.1"
                     /translation="MNPGSPTPRRALVTGGSGDIGGAICRALAVQGCRVYVHANANPQ
                     RAQKLADEIGDGAQAIAFDVTNADATRAALDEILQAGAIDILVHNAGIHDDAPLAGMR
                     AEQWHRVIDVSLHGFFHVAQPLLLPMVRSRFGRIVCVSSVAAQFGNRGQVNYAAAKSA
                     LHGAVKSLAREMASRGITANAVAPGVIAGRLANAAFDAEKIRELVPAGRAGTPEEVAA
                     LVAFLCSDAAAYVNGQIIGVNGGMI"
     gene            complement(1822..>2057)
                     /locus_tag="VFF05_07895"
     CDS             complement(1822..>2057)
                     /locus_tag="VFF05_07895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZW51767.1"
                     /translation="NGAADSAELLAARLKVAMSRPIPIIGSSVTVTLSIGIAYCQGEL
                     SARDLTHLADRALYDATESGRNTHCALRAESAR"
BASE COUNT          288 a          668 c          695 g          406 t
ORIGIN      
        1 gtgctggcgc tgccggcctt tgcacggcgg ccacgcctgt tcgtcatcgc cgcggcggca
       61 ttcgccgcga ttccctacga ttgggaaaac atcgggatgg gcttcgcgaa ttcctatttc
      121 ctgcttgccg gcttcagcgt attcacgatc gtctcggcgg cgcgcgtacg gcgcatcgtg
      181 ccgggtatga tcgcgctgct cgcgttcgcg ctgctcgccg acgtttcgat gggcagcgga
      241 tttttcgccg cggcgattgg tttgtgcatc gtgctcgcgc gctggcatcg cggcgacctg
      301 ggcacgcacg ccgtgctcgc gttcgccacg acgctcgccg cgtgcgcggt atgcggcgta
      361 gtgctgacat ggcacgccac gggccatgca tcgatcggcg cattgcaggc gctgcaagtc
      421 atgctggccg tgttcgtctg gattcccacg gccgtgctgg gctattgctg cctgcgcagc
      481 cacacagtgg atcgctgcga cctcgccttg ctcggcgttt cgctgtgggg gttcgccgag
      541 attctcgcga tcatcgcgat gcgcccggaa attcggctgt ggttcccgat ctcgcgctac
      601 atggaaatcc tcggcattgc tgccttcgcg aacattggtt gcctgatgcg tgcggcgcaa
      661 tcgatgccgg cattgcgccg gctcgcgcgc tggctcttgc ccgcgttcgc ttgcgcaatc
      721 gttctcggca cgccgttcgc cgcatattgg ttcgattggc gcgcgaataa tctggcagca
      781 caagcgcagc gcgtggtgcg ctacgtgaag cgtggtgacg ccgcggcttg gactgccgca
      841 cccgtcacgg agctgccgtt cccgtcgcct gactatctgc gcgccgaact ggatgcggac
      901 gatgtgcgtt acattctcgg cgatgtttac gggacgcgtc cgcagcccgc cctgctgacg
      961 gctttctggc gcggattcgg cgcggatttg cgcgcgtggc ggttcgcgat ctggccagcg
     1021 gcggcgttgc tggcgtggtt gttgctcccg ccagcgtggc gccgcatgcg ttatgcaaca
     1081 attccgcaat gaatcccggt tctcccactc cgcgccgcgc cctcgtcacc ggcggcagcg
     1141 gcgatatcgg cggcgcgatc tgccgcgccc ttgccgtgca gggctgccga gtctacgttc
     1201 acgcgaacgc gaatccgcaa cgcgcacaga agttggcgga cgaaatcggc gacggtgcgc
     1261 aggcgatcgc gttcgatgtg acgaatgcgg acgcgacgcg cgcagcattg gacgaaatcc
     1321 tgcaagccgg cgccatcgac atcctcgtgc acaatgccgg catccacgac gacgcgccgc
     1381 tggccggcat gcgcgcggaa caatggcatc gcgtgatcga tgtctcgttg cacggatttt
     1441 tccacgtcgc gcagccgctg ctgttgccga tggtgcggtc gcgtttcggc cgcatcgtgt
     1501 gcgtgtcgtc ggtcgccgcg cagttcggca atcgcggcca ggtcaattac gcggcggcga
     1561 aatccgcgct gcatggcgcg gtgaaatcgc tggcgcgcga gatggcttcg cgcggcatca
     1621 ccgcgaatgc cgtcgcgccg ggcgtcatcg cgggacggct ggcgaacgcc gcgttcgacg
     1681 cggaaaaaat ccgcgaactg gttccggccg gccgtgccgg cacgccggag gaagtcgccg
     1741 cgctggtcgc gttcctgtgt tcggatgccg ccgcttacgt caacggtcaa atcatcggcg
     1801 tcaacggcgg gatgatctga ctcaccgcgc cgactcggca cgcagcgcgc agtgcgtgtt
     1861 gcgaccggat tccgtcgcgt cgtagagcgc gcgatcggcc agatgggtca ggtcgcgcgc
     1921 cgacagttcg ccttggcaat aggcgatgcc gatactcagc gtgacagtca ccgagctgcc
     1981 gatgatcgga atcgggcgcg acatcgcgac tttcaggcgt gccgcgagca actcggccga
     2041 atcggcggcg ccgttgt
//
LOCUS       DASRVO010000641         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_18773, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000641 DASRVO010000000
VERSION     DASRVO010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18773"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VFF05_07900"
     CDS             complement(<1..1407)
                     /locus_tag="VFF05_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51768.1"
                     /translation="MFASEDGLVASLSSQECIFQVKRSGDTHVMTFQVLQALDQCREF
                     RSLDEHSARIETTIAGLAGKREDIKRVLESLIQRELLVSDDDFIARFARAAARDSADL
                     RGVFIRACDRPEQLSRLLASLTDYERRHRAGRRYIVLDDSSLPAHANDQRDALREFAR
                     TTGCKVHYVGRAESEKLAERFGKAVPVSRGAAKRLLLRESNPRALRFGGGRGWNLAML
                     LSAGSRLALLDDDLILPLRRPDYARPGLDPNPNSRQSARFLANMEQALAVGTAIDTDP
                     FELHLRVCGNTLGDLLASDYPLERGALRGINLGRLDVLSASARIVSTQAASYGSSRSG
                     FGLWLYQLDGDSRAEFWRDRESYVRNCEANFIVQGMAQAQAVAVAGFTPLTVDNGRML
                     PCSNPVGRGEDSLTGALTRFCHPDAVSLSMPEAIGHVQESARKRSDKTLAAHLPHVNY
                     FLRDFVQRQFGVFQAEDAG"
     gene            complement(1516..2370)
                     /locus_tag="VFF05_07905"
     CDS             complement(1516..2370)
                     /locus_tag="VFF05_07905"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZW51769.1"
                     /translation="MSHDLEFTGERFTPECVREIRYEHFARYAFALELARGKRVLDAA
                     CGEGYGSAMLAGTAASVLGVDIAENAIAHARSRYGTQANLHFERGDCTALDAAPGSFD
                     LIVSFETLEHVAAQAELIAGFARVLSDDGILIVSSPDKRTYSDISGFRNEFHVRELYR
                     DELLALLAPHFPQVRLYGQKLLFHSAIWNLEAPPRSVAALTASESAPIPQAGLAYAPL
                     YFIAVCSRRPLPELPDASLFGDREESVYAHYNGEVRRMIAADGRIIELEAEVARLRGE
                     LGQLTPRR"
     gene            complement(2367..>2825)
                     /locus_tag="VFF05_07910"
     CDS             complement(2367..>2825)
                     /locus_tag="VFF05_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZW51770.1"
                     /translation="ANSTGLGTEFELVEFALNGQAAALPIILEQGGALAARVEVISRF
                     GRVPVIAFGLSRADGTTVYGLASDMDGRRPVRVGENRYAMEIAFNDLPLLPGSYLVRA
                     HAMDSEAMRLFSTLERSLTIRGESREFGMVRLAHEWRECAAVESSRIPSP"
BASE COUNT          534 a          898 c          909 g          484 t
ORIGIN      
        1 gcccgcatct tcggcctgga acacgccgaa ctgacgctgc acgaaatcgc gcaggaaata
       61 attcacgtgt ggcagatgcg cggccagagt cttgtccgaa cgcttgcgcg cggattcctg
      121 cacatgacca atcgcttcgg gcatggacaa ggacacggcg tcaggatggc agaatcgagt
      181 cagcgctccg gtcagggaat cttctccgcg cccgaccgga ttcgagcacg gcagcatgcg
      241 cccgttgtcg acggtcagcg gcgtgaagcc cgccactgca accgcctgcg cctgtgccat
      301 gccttgcacg atgaagttcg cctcgcagtt gcgcacataa ctttcacgat cgcgccagaa
      361 ctcggcgcga ctgtcaccgt cgagctgata caaccaaaga ccgaagccgc tgcgcgagct
      421 gccgtagctc gccgcctgcg tggacacgat gcgcgctgat gcggagagca cgtccaggcg
      481 gccgagattt atgccgcgca aggcgccgcg ttcgagcgga tagtcggacg ccaacaaatc
      541 gccaagggtg ttgccacaga cgcgcagatg cagctcgaac ggatcggtgt cgattgccgt
      601 gccgacggcg agcgcctgtt ccatgttagc caggaatcgc gcagattgcc gtgaatttgg
      661 attcggatcg agtcccggcc gcgcgtagtc gggacgccgc agcggcaaaa tcagatcgtc
      721 gtcgaggagc gcaagacggc tgccggccga cagcagcatg gccaggttcc agccgcgacc
      781 gccgccgaag cgcaacgccc gcggattgga ctcgcgcaac agcagccgct tcgcggctcc
      841 gcgcgagacg ggcacggcct tgccgaagcg ctcggccagt ttttccgatt cggcgcggcc
      901 aacgtaatgc actttgcatc cggtcgtgcg cgcgaactcg cgcagggcgt cgcgctggtc
      961 gttcgcatgc gcgggcaacg acgaatcgtc gagcacgatg tagcggcggc cggcgcggtg
     1021 acggcgttcg tagtcggtca acgacgcaag cagacgcgac aactgttcgg gccggtcgca
     1081 ggcgcggatg aagacgccgc gcagatccgc ggagtcgcgc gccgctgcgc gcgcgaaacg
     1141 cgcaatgaaa tcgtcgtcgg ataccaacaa ttcgcgctgg atcaggcttt ccagcacgcg
     1201 cttgatgtct tcgcgtttgc cggcaagccc tgcgatggtg gtctcgatgc gcgcggaatg
     1261 ctcgtccaga ctacggaatt cgcggcactg atccagcgcc tgcagtacct gaaacgtcat
     1321 cacatgggtg tcgccgctac gcttgacctg gaagatgcac tcctggctcg acaacgaggc
     1381 gaccagtccg tcttcggacg cgaacaaggg cgcgttcgga tcgatggccg gtgccgaccg
     1441 cggcgatcgc ggcggatagt tgaattggta gttaagtgtg gtttccattc gattccgggt
     1501 gctttccgga cgccctcagc gccgcggcgt caattgaccg agctcaccgc gcaggcgcgc
     1561 gacctcggct tcgagttcga tgatgcggcc gtcggccgcg atcatgcgac gtacctcgcc
     1621 attgtaatgc gcgtagaccg attcctcgcg gtcgccgaac agactcgcat ccggcagttc
     1681 cggcagcggc cggcgcgaac acaccgcgat gaaatacagc ggcgcgtaag ccagaccggc
     1741 ctgcggtatg ggtgctgatt cggacgccgt gagcgcggca acgctgcgtg gcggcgcttc
     1801 gagattccag atcgccgaat ggaacagcaa tttctgccca tacaggcgca cctgcggaaa
     1861 atgcggtgct agcaaagcca gcaattcgtc gcggtagagt tcgcgcacgt gaaactcgtt
     1921 gcgaaacccg ctgatgtcgc tgtaggtgcg cttgtccggc gaggacacga tgaggatgcc
     1981 gtcgtcgctc agcacgcgtg cgaatccggc gatcaattcg gcttgcgcgg cgacgtgttc
     2041 gagcgtctcg aacgaaacga tcaaatcgaa gcttccgggc gctgcgtcca gtgccgtgca
     2101 gtcgccgcgc tcgaagtgca ggttcgcctg cgtgccgtag cgcgaacgcg cgtgggcaat
     2161 ggcgttttcg gcgatgtcga cgccgagcac gctggctgcg gtaccggcca gcatggcgct
     2221 gccgtagcct tcgccacagg cggcatcgag cacgcgtttg ccgcgcgcca gctcgagcgc
     2281 gaacgcatag cgcgcaaaat gttcataacg gatttcgcgc acacattcgg gagtaaaacg
     2341 ctcgccggtg aattcgaggt cgtggctcat ggcgacggga tccggctcga ttcgacggcc
     2401 gcgcactcgc gccactcatg cgcgagacgc accatcccga attcgcgcga ctcgccgcgt
     2461 atggtcaggc tgcgttccag cgtgctgaac aggcgcatgg cttcggagtc catcgcatgc
     2521 gcacgcacca ggtaactgcc cggcagcagc ggcaaatcgt tgaaggcgat ttccatcgca
     2581 tagcggtttt cgccgacgcg aaccggacgg cgaccgtcca tgtccgaagc cagtccgtat
     2641 accgtggtgc catcggccct gctcaggccg aacgcgatga ccggcacgcg cccgaaacgc
     2701 gagatcactt caacacgtgc tgcgagagca cctccttgct cgaggatgat cggcaacgcc
     2761 gcagcctgtc cgttcaaggc gaattcaacg agttcgaatt ctgtgccgag ccccgtcgaa
     2821 ttcgc
//
LOCUS       DASRVO010000642         1301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_18815, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000642 DASRVO010000000
VERSION     DASRVO010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18815"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VFF05_07915"
     CDS             complement(<1..1025)
                     /locus_tag="VFF05_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZW51771.1"
                     /translation="MIFGSLERLIAFRYLRARRQEGFISVIAIFSLLGIALGVATLIV
                     TMAVMNGFRAELLGRILGFNGHVNVYAVQGGLKDYDAEAAVVRRLPGIVSVRPVIEGQ
                     VMASAGAAASGALVRGMSEKDLASQSLIADHLSPNALAGFKNDAIIVGNRLALRLGIR
                     PGGSITLISPQGTTTVFGTVPRIKTYRVAGTFDVGMYEFDNSVIFMPMETAQTFFNLD
                     KAVTSLEVMVADPDNVRAYDREIDQALGGKARIFDWQQSNLSFFNAVEVERNVMFLIL
                     TLIILVAAFNIVSSMIMLVKDKGRDIAILRTMGATRGMIMRIFILAGASVGLVGTLAG
                     FVLGVEFA"
     gene            complement(1022..>1301)
                     /locus_tag="VFF05_07920"
     CDS             complement(1022..>1301)
                     /locus_tag="VFF05_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HZW51772.1"
                     /translation="LINLRAADAKCTAACDDLYGKLRAAGVDVLYDDRDESAGAKFAA
                     MDLIGLPYQLILGPRGLANNLVEVKTRASGERREMAPEAALNFFAGPR"
BASE COUNT          261 a          402 c          420 g          218 t
ORIGIN      
        1 gcgaattcga cgccgagcac gaagcccgcc agcgtgccga ccaggccgac gctcgcgccg
       61 gcgagaatga agatgcgcat gatcatgccg cgcgttgcgc ccatggtgcg cagaatcgcg
      121 atatcgcgcc ccttgtcctt gaccagcatg atcatgctcg agacgatatt gaacgccgcg
      181 acgagaatga tcagcgtcag gatgaggaac atgacgttgc gctcgacctc gaccgcgttg
      241 aagaaactta agttgctctg ctgccaatcg aagatgcgcg ccttgccgcc aagcgcctgg
      301 tcgatctcgc ggtcataggc gcgcacattg tcgggatcgg ccaccatgac ttcgaggctc
      361 gtcaccgcct tgtcgagatt gaagaaggtt tgcgccgtct ccatcggcat gaagatcacg
      421 gaattgtcga actcgtacat gccgacatcg aaggtgccgg cgacgcgata ggtcttgatg
      481 cgcggcacgg tgccgaagac ggtcgtcgtg ccctgcggcg aaatcagcgt gatgctgccg
      541 cccggccgga tgccgaggcg gagcgcgagc cgatttccga cgatgatggc gtcgttcttg
      601 aatccggcga gggcattggg cgagaggtga tcggcgatga gagattggct cgcgagatcc
      661 ttctcgctca tgccgcgcac gagagcaccc gaggctgccg cgcccgcgct tgccatcacc
      721 tgtccttcga tgaccggccg caccgagacg atgccgggca gacgccggac cacggccgcc
      781 tcggcgtcgt aatccttcag cccgccttgc accgcataga cgttgacgtg gccgttgaag
      841 ccaagaatgc gccccaatag ctcggcgcga aacccgttca tgaccgccat ggtcacgatc
      901 aaggtcgcga cgcccaaggc gatgcccaaa agcgagaaga tcgcgatgac cgagatgaaa
      961 ccttcctggc ggcgcgcgcg cagatagcgg aacgcgatca ggcgttcgag gctgccgaaa
     1021 atcatcgagg accggcgaag aaattcaacg cggcctcggg cgccatctcg cggcgctcgc
     1081 ccgaggcgcg ggtcttcacc tcgacgagat tgttggcaag cccgcgcggg ccgaggatga
     1141 gctgataggg caggccgatc agatccatcg cggcaaattt cgcgccggcg gattcgtcgc
     1201 gatcgtcata gagaacatcg acgccggccg cgcgcagctt gccgtagaga tcatcgcagg
     1261 cggcggtgca tttggcgtcc gctgcgcgca aattgatgag g
//
LOCUS       DASRVO010000643         1014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_122960, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000643 DASRVO010000000
VERSION     DASRVO010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122960"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1014
                     /gene="tgt"
                     /locus_tag="VFF05_07925"
     CDS             <1..>1014
                     /gene="tgt"
                     /locus_tag="VFF05_07925"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZW51773.1"
                     /translation="EIRTPAFMPVGTGGTVKAMYMDQVRGVGADIILGNTYHLMLRPG
                     PERVAALGGLHAFMQWPLPILTDSGGFQVMSLSSLRKLSEQGVTFRSHLDGAMYELTP
                     ERSIEIQALLGADISMQLDECLKLPASREEIERAMQLSLRWGERSKRAFETRAREGYA
                     LFGIVQGGDDLTLRAESARELTDIGFDGYAIGGLAVGEPQAVMLKIAEETTPLLPAGH
                     PRYLMGVGTPHDMLEAVARGVDMFDCVMPTRNGRHGMAFTRFGTINLSNARHANDPRP
                     LDEQNLHPAARTYSRAYLHHLTKANEILGQVLLSTINIAYYQALMAGMREAISAGTFA
                     DFRT"
BASE COUNT          168 a          338 c          330 g          178 t
ORIGIN      
        1 cgagatccgc acgccggcct tcatgccggt cggcaccggc ggcacggtca aggcgatgta
       61 catggaccag gtgcgtggcg ttggcgccga catcatcctt ggcaacacct accatctgat
      121 gctgcggccg gggccggagc gggtcgccgc gctcggcggc ttgcatgcct tcatgcagtg
      181 gccgctgccg atcctcaccg acagcggcgg cttccaggtg atgtcgctgt cgagcttgcg
      241 caagctgtcc gagcagggcg tcaccttccg ttcccatctc gacggcgcca tgtatgagct
      301 cacccctgaa cgctcgatcg agatccaggc gctgctcggc gccgacattt ccatgcagct
      361 cgacgaatgc ctgaagctgc cggcgtcgcg cgaggagatc gagcgcgcca tgcagctgtc
      421 gctgcgctgg ggcgagcgct ccaagcgcgc cttcgagacg cgggcgcgcg aaggctatgc
      481 gctgttcggc atcgtgcagg gcggcgacga tttgacgctg cgcgccgaga gcgcgcgtga
      541 actgaccgat atcggcttcg acggctatgc catcggcggg ctggcggtcg gcgagccgca
      601 ggcggtgatg ctcaagattg ccgaggaaac cacaccgctg ctgccggccg ggcatccgcg
      661 ctatctgatg ggcgtcggta cgccgcacga tatgctggag gcggtggcgc gcggcgtcga
      721 catgttcgac tgcgtgatgc cgacccgtaa cggccggcac ggcatggcgt tcacacgttt
      781 cggcacgatc aatttatcga atgcccgcca cgccaacgac ccgcgtccgc tcgacgaaca
      841 aaacctgcat cccgccgcgc gcacttattc gcgcgcctat ttgcatcacc tgaccaaggc
      901 caatgaaatc ctcggccagg tgctgctgtc gaccatcaac atcgcctatt accaggcgct
      961 gatggccggc atgcgcgaag cgatttcggc gggcacgttt gccgatttcc ggac
//
LOCUS       DASRVO010000644         4544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_123003, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000644 DASRVO010000000
VERSION     DASRVO010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123003"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFF05_07930"
     CDS             complement(<1..873)
                     /locus_tag="VFF05_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HZW51774.1"
                     /translation="MPSVGRREFLKLSVALGASLAWGCAAVHRSSSGWRERRDLYPQG
                     VASGDPDDNSVLLWTRRPYADGRKSATLLVEVADDPAFTRVIAKTTAKVSAESDWTCR
                     VLAGHLPAAGEYWYRFVDEDGNGSRIGRTLTAPNPDDPRPTRFAFVSCQDVCEGYLNA
                     YRKMIFEDERAAVEERIGFVLHLGDFIYEVVQYPDQVKNGHRYDRLITFPIKYPKGKT
                     VARNRFWVPDSLEDYRAAYHAYLQDPDLQDARARWPFVAMWDNHEFSWNGWQSIQEFP
                     GTDGWVPAQTLKVAA"
     gene            complement(1208..1612)
                     /locus_tag="VFF05_07935"
     CDS             complement(1208..1612)
                     /locus_tag="VFF05_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HZW51775.1"
                     /translation="MAAKDDSNKIRNVGPKGAAWLRQVGVRTQDDLVKLGSVDAFMKV
                     KRAGFRPSLNLLYALAGAIENRHWAELPDEQKRALVAAAEANETANPIKTRWQKQVER
                     SERSVEPREESESDSDGGFLDDPDAGHDAHED"
     gene            complement(1614..2108)
                     /locus_tag="VFF05_07940"
     CDS             complement(1614..2108)
                     /locus_tag="VFF05_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZW51776.1"
                     /translation="MFTATAIRADSKPGLYAELAEQARGLLHGERDRIANAANFAALV
                     YHALPDINWAGFYFYDGAELVVGPFQGQPACVRIALGKGVCGTAAVTRQTQVVRDVNA
                     FAGHIACDSASRSEIVVPLFDGDKLIGVWDVDSPHVARFDDDDRAGMETLAGIFMDAL
                     RGAH"
     gene            2162..2887
                     /gene="bioD"
                     /locus_tag="VFF05_07945"
     CDS             2162..2887
                     /gene="bioD"
                     /locus_tag="VFF05_07945"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HZW51777.1"
                     /translation="MNAQAQNRSGVFIAGTDTGIGKSVVSATLLVALNAGGHRAVGMK
                     PVASGCRQAPQGLRNEDTELLIAHGAGAPDYALVNPVALPEAIAPHLAAKHSGMDIRL
                     DPINAAFVALSTNYDFVVVEGVGGWMVPLSDTLMQADLVRALDLPVVLVVGLRLGCLN
                     HAQLSARAIVGDGCHLAGWIANRVDPQMDCVEENLATLRTRLPVPCLGVLPHASAPDP
                     HALAVHLRVAAAAMVSGAYPPAP"
     gene            complement(2853..>4544)
                     /locus_tag="VFF05_07950"
     CDS             complement(2853..>4544)
                     /locus_tag="VFF05_07950"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZW51778.1"
                     /translation="NPFAPRNDSASAAGKKDDADKKDAGKDKDKDKSKSPVQIDDRID
                     FDGLGGRVVRAPIEPDNIAGIAITKKAILYVVGDAPYYGREGAFKTKLVSWSFEERKS
                     STVFEGVGDLSISDDGGTALVKSDDAYKWIDLSAGKPEAKDVKIDGLFALVDPKAEYA
                     EIFGETWRRYRDYYYAKNMHGYDWNAIRAKYEPLLAWVGDRTDLNYVLGQMVAELSSG
                     HAYVSGGDIGLPKKPHVGLVGARFALDAASGQYRIAKILKGQNDEERYRSPLTEVGVD
                     VKQGDYVVAINGQPLTAHDSPYRLLRTAPGQLVQLTVNGKPGSEGAHTVLVKPIDDEL
                     PLNYLAWVEHNRAYVAKASNGEIGYVHIPDMGADGIREFIKNYYPQLRKQGLVVDVRD
                     NGGGNVSAMIIERLSRKLLGLEYARGIDFAGTYPQQTFLGHLVALCNGTTASDGDIFS
                     YMFKQAKLGPLIGERTWGGVVGITDWGPVIDGGHVNVPEFASANTHGQYVVEGHGVDP
                     DIVVEQDVSAQLAGRDPQLDRGIEELKKAIAAKSFAFPPRPADPVKAPVDMRQKP"
BASE COUNT          733 a         1472 c         1473 g          866 t
ORIGIN      
        1 ggccgcaacc ttcagcgtct gcgcgggcac ccagccgtca gtgcccggaa attcctggat
       61 cgactgccag ccgttccagg agaattcgtg gttgtcccac atcgccacga acggccagcg
      121 cgctcgcgca tcctgcagat ccgggtcctg caggtaggcg tggtaggcgg cgcgatagtc
      181 ctcgagcgag tccggcaccc agaagcgatt cctggcgacg gtcttgccct tcggatactt
      241 gatcgggaac gtgatgaggc ggtcgtagcg gtgaccgttc ttgacctggt ccggatactg
      301 tacgacttcg tagatgaagt cgcccaggtg cagcacgaag ccgatacgtt cctcgactgc
      361 ggcgcgctcg tcttcgaaga tcatcttgcg ataggcattc aggtagccct cgcagacgtc
      421 ctggcaactg acgaaggcga agcgcgtcgg tcgcggatca tcggggttcg gcgccgtcag
      481 cgtgcggccg atccggctgc cgttgccatc ctcgtccacg aagcgatacc agtattcgcc
      541 cgccgccggc aggtggccgg ccagcacgcg acaggtccag tcggactcgg ccgacacctt
      601 tgccgtcgtc ttcgcgatca cgcgcgtgaa cgccgggtcg tccgccactt ccaccagcaa
      661 ggtggcgctc ttgcgcccgt ccgcatacgg gcgccgcgtc cacagcagga cgctgttatc
      721 gtcaggatca cccgacgcga cgccttgcgg atacaggtcg cggcgctcgc gccagccgct
      781 cgatgaccga tgcacggccg cgcagcccca ggccagcgac gcgcccaacg cgacagagag
      841 cttcaggaat tctcgtcggc ccacgcttgg catgttcgtc ccctccctac agcggatcgc
      901 gataatcgat acgccgaaaa ctagacccga tcacgggttt gggagcgtgc cgaaagtacc
      961 gcggggccgt gtcagcaatc gccccacacc agacactcgc aagcaacagc tatcgagtcc
     1021 accaccgaag acgtgctgcc tgaaatccgg cgcgcgccga agcggcgcga ggatgcacgc
     1081 gattacgcgg tgatgcggtg atctcattag acgatggaga ttggttgact agaactgccg
     1141 ctgatgaaag attctctaga attcccggta gcagagaata tgtgtccatg cgatctaaca
     1201 gatccgctca atcctcgtgc gcgtcgtggc ctgcgtcggg atcgtccagg aatccgccgt
     1261 cgctgtcgga ctcggattcc tcgcgcggtt cgaccgaacg ctcgctgcgt tcgacctgct
     1321 tctgccagcg cgtcttgatc ggattggcgg tttcgttcgc ttcggccgca gcaaccagcg
     1381 cgcgcttttg ttcgtcgggc agttcggccc agtggcgatt ctcgatcgca ccggccaagg
     1441 cgtagagcag attcaggctg ggccggaagc cggcgcgctt gaccttcatg aacgcatcga
     1501 ccgaaccgag cttgacgagg tcgtcctgcg tgcgcacgcc gacctggcgc agccacgctg
     1561 cacctttcgg cccgacgttg cgaatcttgt tgctgtcgtc cttggccgcc atctcaatgc
     1621 gccccgcgca gcgcatccat gaagataccg gcgagcgttt ccatgccggc gcggtcgtcg
     1681 tcatcgaaac gcgcaacatg cggactgtcc acgtcccaca cgccgatcag cttgtcgcca
     1741 tcgaacagcg gcacgacgat ttccgagcgc gaggccgaat cgcaggcgat gtgcccggcg
     1801 aacgcgttga cgtcgcgcac gacctgggtt tgccgcgtga ccgccgcggt gccgcacacg
     1861 cccttgccaa gcgcgatgcg cacgcaggcg ggttggccct ggaacgggcc aaccacgagc
     1921 tctgcgccgt cgtagaaata gaatccggcc cagttgatgt ccggcaacgc gtgataaacc
     1981 agcgcggcga aattcgccgc attcgcgatg cggtcgcgct cgccgtgcag caggccgcgc
     2041 gcctgttcgg cgagttcggc gtaaagcccg ggcttgctgt cggcacggat ggcggtggcg
     2101 gtgaacatgc cggcattcta gcgtgtggcg aatatcggcg cagcgaatta tcctgcgaga
     2161 cgtgaacgca caggctcaaa atcgatcagg cgtcttcatc gccggcaccg acaccggcat
     2221 cggcaagtcc gtggtgtcgg caacactgct ggtcgcactc aatgcaggcg gacatcgcgc
     2281 cgtagggatg aaacccgtcg ccagcggctg ccggcaagcg ccgcaggggt tgcgcaacga
     2341 ggacaccgaa ctgctgatcg cgcacggcgc cggtgcgccg gattacgcgc tggtcaatcc
     2401 ggtcgcactg cccgaggcga tcgcgccgca tctggcggcg aaacattcgg gcatggatat
     2461 ccggctcgat ccgatcaatg ccgcatttgt cgcattgtcc acaaattacg attttgtcgt
     2521 ggttgaaggc gtgggcggct ggatggtgcc attgtccgac acgttgatgc aggccgatct
     2581 cgtgcgagcg ctggatttgc cggtggtcct cgtcgtcggc ctgcgcctgg gttgcctgaa
     2641 tcacgcccag ctttccgcgc gcgcgatcgt cggcgacggc tgccatctcg ccggctggat
     2701 cgcgaaccgc gtcgatccgc agatggattg cgtcgaggag aacctcgcga cgctgcgcac
     2761 gcgtctgccg gttccgtgcc tgggcgtgct gccgcacgcc agcgcgcccg atccgcacgc
     2821 gctggcggtc catctgcgcg tggcagccgc ggctatggtt tctggcgcat atccaccggc
     2881 gccttgaccg gatccgccgg gcgcggcggg aacgcgaacg acttggccgc gatcgccttc
     2941 ttgagctcct cgatgccgcg gtcgagctgc gggtcgcggc cggcgagctg cgccgaaacg
     3001 tcttgctcga cgacgatgtc gggatcgaca ccatggcctt cgacgacgta ctgcccgtgt
     3061 gtgttcgcgc tggcgaattc aggcacgttg acgtggccgc catcgatgac cggaccccag
     3121 tcggtgatgc cgaccacgcc accccaggtg cgctcgccga tcagcggacc gagcttggcc
     3181 tgcttgaaca tgtacgagaa gatgtcgcca tcggacgcgg tcgtgccgtt gcacagcgcg
     3241 acgaggtggc ccaggaaagt ttgctgtgga taggtgccgg caaaatcgat gccgcgcgcg
     3301 tactcgagtc cgagcagttt cctggacagg cgctcgatga tcatcgccga gacgttgccg
     3361 ccgccattgt cgcgcacatc gacgacgaga ccttgcttgc gcagttgcgg gtagtaattc
     3421 ttgatgaact cgcggattcc gtcggcaccc atgtccggga tgtgcacata gccgatttcg
     3481 ccgttgctgg ccttggcgac atacgcgcgg ttgtgctcga cccaggcgag gtagttgagc
     3541 ggcagctcgt cgtcgatggg ttttaccagc acggtatgcg cgccttcgct gccgggtttg
     3601 ccgttgacgg tgagctgcac cagctgcccc ggcgcggtgc gcagcaggcg gtatgggctg
     3661 tcgtgcgcgg tcagcggctg gccgttgatg gcgacgacat agtcgccttg cttgacgtcg
     3721 acgccgacct cggtcagcgg cgagcggtag cgctcctcgt cgttctggcc cttgaggatt
     3781 ttcgcaatgc ggtactggcc gctcgccgcg tcgagagcga agcgcgcgcc aaccaggccg
     3841 acgtgcggct tcttcggcag gccgatgtcg ccgccgctga cgtaggcgtg cccgctcgac
     3901 agctccgcga ccatctggcc gagcacgtag ttgaggtcgg tgcggtcgcc gacccaggcc
     3961 agcagcggct cgtacttggc gcggatcgcg ttccagtcgt agccgtgcat gttcttcgca
     4021 tagtagtagt cgcggtagcg acgccacgtt tcgccgaaga tctcggcata ttcggccttc
     4081 ggatcgacga gcgcgaacag gccgtcgatc ttgacgtcct tcgcctcggg tttgccggcg
     4141 ctgaggtcga tccacttgta ggcgtcgtcg ctcttgacca gcgcggtgcc gccgtcgtcg
     4201 gaaatcgaca ggtcgccgac gccttcgaag acggtgctgc tcttgcgctc ctcgaacgac
     4261 cagcttacga gcttggtctt gaacgcgcct tcgcggccgt agtacggtgc gtcgccgacg
     4321 acgtagagga tcgccttctt cgtgatcgcg atgccggcga tgttgtcggg ttcgatcggc
     4381 gcgcgcacga cgcgtccgcc cagaccgtcg aagtcgatgc ggtcgtcgat ctgcacgggc
     4441 gatttcgatt tgtccttgtc cttgtccttg ccggcgtcct tcttgtcggc atcgtccttc
     4501 ttcccggccg ccgaggcact gtcgttgcgc ggcgcgaacg ggtt
//
LOCUS       DASRVO010000645         1571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_19036, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000645 DASRVO010000000
VERSION     DASRVO010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19036"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VFF05_07955"
     CDS             <1..233
                     /locus_tag="VFF05_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HZW51779.1"
                     /translation="NCLIGGGAGIVGHIRIADHVTITARTLVTHSIDAPGEYSSGTPV
                     QDNRAWRRNAARFKHLDELARRVGQLEKDPHK"
     gene            230..697
                     /gene="fabZ"
                     /locus_tag="VFF05_07960"
     CDS             230..697
                     /gene="fabZ"
                     /locus_tag="VFF05_07960"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZW51780.1"
                     /translation="MTQTGQDVSLPLDVERIMGFLPHRFPFLLVDRVVDFEHSRRLVA
                     LKNVTVNEPHFQGHFPGHPVMPGVLIIEALAQASGLLVFLSTPKHETQPMYYLVKVDN
                     AKFTRMVVPGDQLMLHVELKRMLRGMGLFACRALVGGEQVAEAELLCAGRNGA"
     gene            694..1473
                     /gene="lpxA"
                     /locus_tag="VFF05_07965"
     CDS             694..1473
                     /gene="lpxA"
                     /locus_tag="VFF05_07965"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HZW51781.1"
                     /translation="MIHPTALVDASAKLGANVSVGPYAVIGAEVEIGDGTTIGPHAVI
                     QGPTRIGRDNRIWQFASVGAEPQDKKFRGERSELVIGDRNTIREFVTINRGTGGGAGA
                     TRVGDDNWLLAYTHIAHDCHVGSHCVFSNNATLAGHVEIGDHVILSGFAGVHQFCRVG
                     EHAFIGMGVLLTGDVPPFLMIATDADVQGRPRGINSEGLKRRGFSPERIAAIKRAYKT
                     LYVAKLPFEEARVELARAAQEAPDVGRMLDFIERGQRSLMR"
BASE COUNT          274 a          550 c          488 g          259 t
ORIGIN      
        1 gcaactgcct gatcggcggc ggcgccggca tcgtcggcca catccgaatt gcggaccacg
       61 tcacgatcac ggcacgaacc ctcgtcacgc actccatcga tgcgcccggg gagtattcat
      121 ccggcacgcc ggtgcaggat aatcgcgcct ggcgtcgcaa cgcggcgcgc ttcaagcatc
      181 tggacgaact cgcccgccgc gtggggcaac tggaaaagga tccgcacaaa tgacccaaac
      241 cggacaagac gtttcgcttc cgctcgatgt cgagcgcatc atgggtttcc tgccgcaccg
      301 cttcccgttc ctgctcgtcg atcgcgtggt cgatttcgag cattccaggc gcctggtcgc
      361 gctgaagaac gttaccgtca acgagccgca cttccagggc cacttccccg gccatccggt
      421 aatgcccggc gtgctgatca tcgaagcgct ggcgcaagcc tccggcctgc tcgtgttcct
      481 ttccacgccg aagcacgaaa cccagccgat gtactacctg gtcaaggtcg acaacgccaa
      541 gttcacgcgc atggtggtac cgggcgacca gttgatgctg cacgtcgagc tcaagcgcat
      601 gcttcgcggc atgggcctgt tcgcctgccg cgcactggtt gggggcgagc aggtggccga
      661 agccgaactg ttgtgcgccg ggcgcaacgg cgcatgatcc atcccaccgc gctggtcgac
      721 gcaagcgcaa aactcggcgc caacgtcagc gtcggcccct acgccgtgat tggcgccgag
      781 gtggaaatcg gcgacggcac cacgatcggc ccgcatgcag tgatccaggg gcccacgcgc
      841 atcggccgcg acaaccgcat ttggcaattc gcctcggtcg gcgccgagcc gcaggacaag
      901 aaattccgcg gcgagcgcag cgagcttgtc atcggcgatc gcaacacgat ccgcgaattc
      961 gtgacgatca accgcggcac cggcggcggt gccggcgcta cccgcgtcgg cgacgacaac
     1021 tggctcctcg cctacacgca catcgcgcac gactgccacg tcggcagcca ttgcgtgttt
     1081 tcgaacaacg ccacgctcgc cggtcacgtc gagatcggcg atcacgtcat cctctcgggt
     1141 ttcgccggcg tgcaccagtt ttgccgcgtc ggcgaacacg ccttcatcgg catgggcgtg
     1201 ctgctcaccg gcgatgtgcc gccgttcctg atgatcgcca ccgacgcgga cgtgcagggc
     1261 cgcccgcgcg gcatcaattc ggaaggcttg aagcgccgcg gcttttcgcc cgagcgcatc
     1321 gccgcgatca agcgcgccta caagacgctg tacgtcgcca agctgccatt cgaggaagcc
     1381 cgtgtggaac ttgcgcgcgc cgcgcaggaa gcgccggacg tggggcgcat gctcgacttc
     1441 atcgagcgcg ggcagcgcag cctgatgcga tgacgccgga cgcgagtccg ctgttcgtcc
     1501 tcgtcgccgg cgaagcctcg ggcgacctgc tcggcgctgg actaatcgat gccctgcgcc
     1561 aaagcttccc t
//
LOCUS       DASRVO010000646         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_71118, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000646 DASRVO010000000
VERSION     DASRVO010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71118"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VFF05_07970"
     CDS             <1..181
                     /locus_tag="VFF05_07970"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437117.1"
                     /note="with CysN catalyzes the formation of
                     adenylylsulfate from sulfate and ATP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HZW51782.1"
                     /translation="VRFRTLGCYPLTGAVRSDAANLDQIIAEMRASHTTERQGRAIDH
                     DPASSMEAKKREGYF"
     gene            181..>1170
                     /locus_tag="VFF05_07975"
     CDS             181..>1170
                     /locus_tag="VFF05_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006450708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZW51783.1"
                     /translation="MATASTPDLLRLITCGSVDDGKSTLLGRLLHDAGLLRDDELAAL
                     GDPPDFSRLLDGLALEREQGITIDVAYRYFSTAKRDFIVADCPGHAQYTRNMATGAST
                     ASLALVLVDARKGVLTQTRRHSYICALLGIRRVVLAVNKMDVAGFDAATFARIETEYR
                     ELADKLGIDSIAAIPLSALEGDNLVQRSPRMPWYRGPTLLEFLEQVPAQADADAAPMR
                     LPVQWVNRHGQDFRGYAGNLVSGRVAVGDAVVALPSARRTQVTRVFDGDKDVDSAFAG
                     QAITLTLADEIDIARGDVIAATHDAPEVADQFAAHILWMDEAPLLPGRLYFLQL"
BASE COUNT          189 a          405 c          384 g          192 t
ORIGIN      
        1 ggtgcgcttc cgcacgctgg gctgctatcc gctgaccggc gcggtgcgct cggatgcggc
       61 gaatctggat cagatcatcg ccgagatgcg cgcctcgcac acgaccgagc gccagggccg
      121 cgccatcgac cacgatccgg cctcgagcat ggaagcgaaa aagcgcgagg gttatttctg
      181 atggccaccg catccacgcc ggacctgctg cgcctgatta cctgcggcag cgtcgacgac
      241 ggcaagagca cgctgctcgg ccgcctgctg cacgacgccg gtctgttgcg cgacgacgag
      301 ctcgccgcac tcggcgatcc gccggatttc tcgcgcctgc tcgacggcct cgcgctcgaa
      361 cgcgagcagg gcatcaccat cgacgtcgcg tatcgttatt tcagcacggc aaaacgtgat
      421 ttcatcgttg ccgactgtcc cggccacgcc cagtacacac gcaacatggc gaccggtgcc
      481 tcgaccgcgt cgctggcgct ggtgttggtc gacgcgcgca agggcgtgct cacgcagacg
      541 cgtcggcaca gctacatctg cgcgctgctc ggcatccgcc gcgtggtgct ggcggtgaac
      601 aagatggacg tggccggatt cgacgcggcg actttcgcgc gcatcgaaac cgaataccgc
      661 gaactcgcgg acaagcttgg catcgactcc atcgccgcga ttccgctgtc ggcgctggaa
      721 ggcgacaacc tcgtgcagcg ctcgccgcgc atgccgtggt atcgcgggcc gacgctgctc
      781 gaatttctcg agcaggtgcc ggcgcaggcc gatgccgatg ccgcgccgat gcgcctgccg
      841 gtgcagtggg tcaatcggca tggccaggat ttccgcggct acgcgggcaa tctcgtttcc
      901 ggccgcgtgg cggtcggcga tgccgtcgtt gcgctgccct cggcacggcg cacgcaggtg
      961 acgcgcgtgt tcgacggcga caaggatgtg gacagcgcct tcgccggaca agccatcacc
     1021 ctaacgctcg ccgacgaaat cgacatcgcc cgcggcgacg tcatcgccgc gacgcacgac
     1081 gcgcccgaag tcgccgacca gttcgccgcg cacattttgt ggatggacga agcgccgctg
     1141 ctgccggggc ggctgtattt cctgcaactg
//
LOCUS       DASRVO010000647         1235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_147951, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000647 DASRVO010000000
VERSION     DASRVO010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147951"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1235
                     /locus_tag="VFF05_07980"
     CDS             <1..>1235
                     /locus_tag="VFF05_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZW51784.1"
                     /translation="AYAGALAALLGIDMDIIGEMLREKFSRKRALLDANFTAIRLGHD
                     YAKGEYDCPLPFHLEPMDANANRILLDGNSAAALGAVYAGATVGAWYPITPSTSLMDA
                     FRGFCQKYRRDESANRNRFAIIQAEDELAAAGMVIGAGWAGARAFTSTAGPGISLMNE
                     FIGLAYFAEIPVTIIDVQRGGPSTGMPTRTQQSDVLACAYASHGDTKHVLLFPQDPHE
                     CFAFSAQALDLADRLQTPIFVLTDLDIGMNQRLCAPFDWDDAREYDRGKVMTAEELEA
                     GKYFGRYKDVDGDGIPWRTYPATHPKRGSFFTRGTSKNPEARYSERGDDYIYNMQRLL
                     KKFDTAKSLVPQPILHAAKQKTRFGAIYYGSTSPAMDEALAKFEADGVHVDALRVRAF
                     PFPDSVAEFIAAHDTVFVV"
BASE COUNT          213 a          407 c          404 g          211 t
ORIGIN      
        1 cgcctatgcc ggcgcgctgg ccgcgttgct cgggatcgac atggacatca tcggcgagat
       61 gctgcgcgag aaattctcga ggaagcgcgc gctgctcgac gccaacttca cggcgatccg
      121 gctgggccac gactacgcga agggcgagta cgattgtccg cttcccttcc atctcgagcc
      181 gatggacgcg aacgcgaacc gcattttgct cgacggcaac tcggcggccg cgctgggggc
      241 cgtgtacgcc ggcgcgacgg tcggcgcgtg gtacccgatc acgccgtcga cgtcgctgat
      301 ggacgcgttc cgcggcttct gccagaagta ccgccgcgac gagtccgcca accgcaaccg
      361 cttcgcgatc atccaggcgg aggacgagct ggcggccgcc ggcatggtga tcggtgccgg
      421 atgggcgggc gcccgtgcgt tcacctccac ggccggcccg ggcatctcgc tgatgaacga
      481 gttcatcggc ctggcgtatt tcgccgagat tccggtgacc atcatcgacg tgcagcgtgg
      541 cgggccctcg accggcatgc ccacgcgcac gcagcagtcc gacgtgctcg cctgcgccta
      601 cgcctcgcat ggcgacacca agcatgtgct gctgttcccg caagacccgc acgagtgctt
      661 cgcgttttcc gcgcaggcgc tggatctggc cgaccgcctg cagacgccga tcttcgtcct
      721 gaccgacctc gacatcggca tgaaccagcg cctgtgcgcg ccgttcgatt gggacgacgc
      781 gcgcgagtac gaccgcggca aggtgatgac ggccgaagaa ctcgaagccg gcaagtattt
      841 cgggcgttac aaggacgtcg atggcgacgg cattccgtgg cgcacgtatc cggcgacgca
      901 tcccaagcgc ggcagttttt tcacgcgcgg cacgagcaag aatcccgaag cgcgttattc
      961 ggagcgcggc gacgattaca tctacaacat gcagcgcctg ttgaagaagt tcgacacggc
     1021 gaaatctctg gtgccgcaac ccatcctgca tgcggcgaaa cagaagacgc gtttcggcgc
     1081 gatctactac ggctcgacca gtccggcgat ggacgaggcc ctggccaagt ttgaagcgga
     1141 cggcgtgcac gtggacgcat tgcgcgtgcg tgcgtttccg ttcccggatt cggtcgcgga
     1201 attcatcgcg gcgcacgaca cggtcttcgt ggtcg
//
LOCUS       DASRVO010000648         1155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_123142, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000648 DASRVO010000000
VERSION     DASRVO010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123142"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..>1155
                     /locus_tag="VFF05_07985"
     CDS             24..>1155
                     /locus_tag="VFF05_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZW51785.1"
                     /translation="MLNIIWLGFFLVAMLTGLAQWLLGGHPQVFAAMVDSLFEMAKLS
                     VDVMIVLFGTLALWLGFLRIAERAGLVEKLARFLGPLFAHLMPEVPRGHPAIGLITLN
                     FAANMLGMDNAATPIGLRAMRELQTLNPSADTASNAQVLFLVLHSSSLVLLPVTIFMY
                     RVQQGAHDPTLVFVLILLATCASALAGLLSVSFVQKLKLWNPVVLAYLGGFAVLFAGF
                     VALLASMTPVALAAFSSLLGNLAMLGIIALFLIAGAVRRVPLYECFVDGAKQGFDVAR
                     DILPYLVAMLYAIGVLRASGALDFLLGGIRWLAESAHLDTRFVDALPTALVKPFSGSA
                     ARAMLIETMQAKGVDSFPALAAATVQGSTETTFYVIAVYYGAV"
BASE COUNT          155 a          373 c          365 g          262 t
ORIGIN      
        1 cgccgttcgg gcttgatgcg cgcatgctga atatcatctg gctcggcttt tttctcgtcg
       61 cgatgctgac cggcctcgcg cagtggctgc tcggcggcca tccgcaggta ttcgcggcga
      121 tggtcgacag cctgttcgag atggccaagc tgtcggtcga cgtgatgatc gtgctgttcg
      181 gcacgctagc cctgtggctg ggattcctgc gcattgccga gcgtgccgga ctggtggaaa
      241 aactcgcgcg ctttcttggc ccgctgttcg cgcacctgat gccggaagtg ccgcgcggac
      301 atccggcgat tggactcatc acgctcaact tcgccgcgaa catgctcggc atggacaatg
      361 ccgcgacgcc gatcggcctg cgcgcgatgc gcgaactgca aacgcttaat cccagcgcgg
      421 acacggcgag caatgcgcag gtcctttttc tggtgctgca ttcctcttcg ctggtgctgc
      481 tgccggtgac gatcttcatg tatcgcgtgc agcaaggcgc gcacgatccg acgctggtat
      541 tcgtgctgat cctgcttgcg acttgcgcct ccgcgctggc cggattgctc agcgtgtcct
      601 tcgtgcagaa attgaaactg tggaatcctg tcgtgctcgc gtacctgggc ggattcgccg
      661 tgctgttcgc gggattcgtc gccctgctcg cgtcgatgac gcctgtggcg ctcgcggcgt
      721 tttcgtccct gctcggcaac ctcgcgatgc tcggcatcat cgcgctgttc ttgatcgccg
      781 gcgctgttcg gcgcgtgccc ttgtacgagt gtttcgtcga cggtgccaag cagggtttcg
      841 acgtggcccg cgacatcctg ccttatctcg tcgccatgct atatgcgatc ggcgtgttgc
      901 gcgcctccgg cgcgctggat ttcctgctgg gcggcatccg ttggctggcc gaatccgcgc
      961 atctggacac gcgcttcgtc gacgcactgc cgaccgcgct agtgaaaccg ttctcgggtt
     1021 ccgccgcgcg cgccatgctg atcgagacga tgcaggccaa gggcgtcgac agtttccccg
     1081 cgcttgccgc cgcgaccgtg cagggcagca ccgaaaccac gttctacgtg attgccgtct
     1141 actacggcgc ggtcg
//
LOCUS       DASRVO010000649         1450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_19081, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000649 DASRVO010000000
VERSION     DASRVO010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19081"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFF05_07990"
     CDS             complement(<1..979)
                     /locus_tag="VFF05_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011269543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZW51786.1"
                     /translation="MAAVPVLDEKRFAYLKPSDFARLADAYRFSEAAHAGQKRQSGEP
                     YVSHPLAVAEILAGWRLDGQTLMAAFLHDVMEDASVTKTEISDTFGRPVAELVDGVSK
                     LDKIEFQSAEDAQAGNFRKMLLAMARDLRVILIKLADRLHNMRTLSAMAPESRRRIAR
                     ETLDIYALIAQRLGMNKFKSELQELGFRMLYPMRHRVIAARIRSVLGNRREAMGKIEA
                     ALTQRLASEHVVARVVGRIKSPWSIYSKMRNEHKSFAQLMDVYGFRVVVDNVTSCYMA
                     LGAVHALYKPVDRRFKDFIAIPKANGYQSLHTVLLGPFGAPIEVQIRTAE"
     gene            complement(1045..1278)
                     /gene="rpoZ"
                     /locus_tag="VFF05_07995"
     CDS             complement(1045..1278)
                     /gene="rpoZ"
                     /locus_tag="VFF05_07995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZW51787.1"
                     /translation="MARITVDDCLKKIPNRFQLTLAATYRARQITAGASPLLETNKDK
                     ATVIALREIASGKVGTEVLKRNPTPPAAPSSPL"
BASE COUNT          236 a          464 c          475 g          275 t
ORIGIN      
        1 tctcggccgt gcggatctgc acttcgatcg gcgcaccgaa cgggcccagc agcacggtgt
       61 gcagcgactg gtagccgttt gccttgggga tcgcgatgaa atccttgaaa cgccggtcga
      121 ccggcttgta cagcgcgtgc accgcgccga gcgccatgta gcagctggtc acgttgtcga
      181 cgaccacgcg aaatccgtag acgtccatca gctgggcgaa actcttgtgt tcgttgcgca
      241 tcttcgaata gatgctccac ggcgacttga tccgccccac cacgcgcgcc acgacatgct
      301 cggacgccag tcgctgggtc agcgccgcct cgatcttgcc catcgcctcg cgccggttgc
      361 cgagcacgct gcggatgcgc gccgcgatca cgcgatggcg catcgggtaa agcatgcgga
      421 agccgagttc ctgcagctcg gacttgaact tgttcatgcc caggcgctgg gcgatcagcg
      481 cgtagatgtc gagcgtctcg cgggcgatgc ggcggcgcga ttcgggcgcc atcgccgaca
      541 acgtgcgcat gttgtgcagg cggtcggcga gcttgatcag gatcacgcgc aagtcgcgcg
      601 ccatcgccag cagcatcttg cggaaattgc ctgcctgcgc gtcctcggcg gactggaact
      661 cgatcttgtc cagcttggac accccgtcga ccagctcggc gaccggcctg ccaaaggtgt
      721 ccgagatttc cgtcttggtg accgaggcgt cctccatcac gtcgtgcagg aacgccgcca
      781 tcagcgtctg gccgtccagg cgccacccgg ccaggatttc ggccaccgct aggggatgcg
      841 aaacgtacgg ttccccggac tggcgtttct gtccggcatg cgccgcctcc gagaagcggt
      901 aggcatccgc caggcgcgca aagtccgacg gcttcaggta agcgaagcgc ttctcgtcaa
      961 ggacgggtac ggccgccatg cgggggagcg tacccgatcg gactcccctt gcgaacaccc
     1021 cggggatccg gccggaggct gaactcagag cggtgagctg ggcgccgccg gcggcgtcgg
     1081 gttgcgcttg aggacttcgg taccgacctt gccgctcgcg atctcgcgca gcgcgatcac
     1141 cgtcgccttg tccttgttcg tttcgagcag cggcgaggcg ccggcggtga tctggcgtgc
     1201 gcggtaggtc gccgccagcg tcagctggaa gcggttgggg atcttcttca ggcaatcgtc
     1261 gacggtgatg cgggccatgt ttcgcgttcc attcagaaaa gttcgccgat acttgcaagt
     1321 tgccgctggg tgacgaggcg ttcggcgcgg atgattgcga gcaagtctcg cttggcaacc
     1381 tcgaactctt tgttaattat aacataatcc gattccgtga cgtgcgagat ctcgtcccgc
     1441 gcggcggcca
//
LOCUS       DASRVO010000650         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_305673, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000650 DASRVO010000000
VERSION     DASRVO010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305673"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VFF05_08000"
     CDS             <1..1666
                     /locus_tag="VFF05_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW51788.1"
                     /translation="AIFGGTLAGIAGFVPSHIVENAASPPPRLDRIRVRRAGNDIVLD
                     TADVELGWRDRDLRLTARLLAFADPAAQRYQWKLGGLDTDWIDGGNRGEREYARLPPG
                     EYFLRVRGADAGGAWSAPSAPLRVRVAPPPWSTPFAYVGYAALLALAVALAFRAYRRR
                     LKRRHSWELAKQQREFALNANAAKSDFLANMGHEIRTPMTGVLGMAELLLRTNLDGKQ
                     HGYAQAIQDSGRLLLRLVNDSLDLARIEAGKLQLEMTPFELRALLRQVEALAQPQAAK
                     RGLAWTLSIADDVPHWLTGDALRIEQILLNLVNNALKFTERGGIGLSVRRGRAAGIAF
                     KVDDSGPGIAAAERKRLFRRFEQADGPQRRGGSGLGLAICRELVARMGGSVELDSEPG
                     CGSTFLVELPLPESEPHGIARNDKVEIVASPTVAASRRILLVEDDATVAATLAGLLEA
                     QGHGVRHAEHGLAALGELAAADYDAALLDLDLPGVDGLTLARMIRAGEAKDDKPSLRL
                     IGISARSRGDEEALCRAAGMDAFVRKPVTGEMLAEVLRHDDPAPIR"
     gene            complement(1663..2130)
                     /locus_tag="VFF05_08005"
     CDS             complement(1663..2130)
                     /locus_tag="VFF05_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51789.1"
                     /translation="MTRRPAVFAIGVGGGIAATLDIAYALTFWFCYRGVAPTAVLHTI
                     AGGLLGLPVARAGGAGTALLGLSLHYAIAFAMAAAFFIVSRYFRFLTRQPIIAGVLYG
                     IALYAIMNFVVLPLSALPPHAWKPTLPALTDLCSHMFFVGLPIALATRRARAG"
     gene            complement(2127..>2861)
                     /locus_tag="VFF05_08010"
     CDS             complement(2127..>2861)
                     /locus_tag="VFF05_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZW51790.1"
                     /translation="PETDLRYVMDAVRLIRRELHDKVPLIGFAGSPWTLACYMIEGGG
                     SSSFAKAKALALDDSATMHKLLDVVARSVAAYLAAQAAAGAQALMVFDTWGGLLAPDM
                     FREFSLRYLAQIAQSLQIHNAAKSIPLILFSKGANAHLETLADTGCAALGLDWTIALD
                     DARHRVGNRVALQGNLDPAVLHAQPETIRAEVKKTLDSFGPHPGHVFNLGHGITPDVD
                     PEHLRVLIDAVHDSSAQPAQSASKAR"
BASE COUNT          466 a          911 c         1024 g          460 t
ORIGIN      
        1 cgcgatcttc ggcggcaccc tggccggcat cgcgggcttt gtcccatcgc acattgtgga
       61 aaatgccgca tcgccgccgc cgcgactgga tcgcatccgg gtgcgccgcg ccgggaatga
      121 cattgtgctg gatacggccg acgtcgaact cggctggcgc gatcgcgacc tgcgcctgac
      181 cgcgcgcctg ctggccttcg ccgatccggc cgcgcagcgc tatcagtgga agctgggcgg
      241 gttggatacg gattggatcg acggcggcaa tcgcggcgag cgcgaatacg cgcggttgcc
      301 gcccggcgag tatttcttgc gcgtgcgcgg cgcggatgcc ggcggcgcgt ggagcgcgcc
      361 ttccgcgccg ctgcgcgtgc gcgtggcgcc accgccgtgg tccacgccgt tcgcctacgt
      421 cggttatgcg gcgctgctgg cgttggccgt cgcgctcgcc tttcgcgctt atcgccggcg
      481 cctgaaacgc cggcattcct gggaactggc caagcagcag cgcgagttcg cgttgaacgc
      541 gaacgcggcg aaatccgatt tcctcgccaa catgggccat gagatccgca ctcccatgac
      601 cggcgtgctc ggcatggccg aactgctgtt gcgcaccaac ctcgacggca aacagcatgg
      661 atacgcgcag gcgatccagg attcggggcg attgctcctg cgcctggtca acgattcgct
      721 ggacctggcg cgcatcgagg ccggcaagct gcaactggaa atgacgccgt tcgagctgcg
      781 cgccttgctg cgccaggtcg aagcgctcgc gcaaccacag gccgcgaaac gcgggctggc
      841 gtggacgcta tcgatcgcgg acgacgtgcc gcactggttg accggcgatg cgctgcgcat
      901 cgaacagatt ctgctcaacc tggtcaacaa cgcgctcaag ttcaccgagc gcggcggcat
      961 cggattatcg gtgcggcgcg gcagagccgc cggcatcgcg ttcaaggtcg acgatagcgg
     1021 tccgggtatc gctgcggccg aacgcaagcg cctgttccgc cgcttcgagc aggccgacgg
     1081 gccgcagcgt cgcggtggca gcggcttggg cctggcgatt tgtcgcgaac tcgtcgcgcg
     1141 catgggtggc agcgtcgaac tggacagcga gccggggtgt ggcagcacgt tccttgtcga
     1201 actgccattg cccgaatccg agcctcacgg catcgcgcgc aacgacaaag tggaaattgt
     1261 cgcatcgccg acggtcgccg cgtcgcgtcg cattctgctg gtcgaggacg atgcgaccgt
     1321 cgccgccacg ctcgccggtt tgctcgaagc gcagggccat ggcgtgcgcc acgccgagca
     1381 cgggctggcg gcgctgggcg aactggccgc cgcggattac gacgcggcgc tgctcgatct
     1441 ggacctgccc ggcgtggacg ggctgacgct cgcgcgcatg atccgcgcgg gcgaggcgaa
     1501 ggacgacaag ccgagcttgc gcctgatcgg catcagcgcg cgttcgcgcg gcgacgaaga
     1561 agcgttgtgc cgcgccgccg gcatggatgc atttgtgcgc aagccggtga cgggggagat
     1621 gctcgcggag gttctacgcc acgacgatcc cgcgccgatt cgctagcctg cgcgcgccct
     1681 gcgcgtggcc agcgcgatcg gcaggccgac gaagaacatg tgcgagcaca ggtcggtgag
     1741 cgcgggcagc gtgggtttcc aggcatgcgg cggcagcgcg gacagcggca ggacgacgaa
     1801 attcatgatg gcgtatagcg cgatgccgta cagcacgccg gcaatgatcg gctggcgcgt
     1861 gagaaatcga aagtaccggc tgacgatgaa gaatgccgcc gccatggcga aggcgatggc
     1921 gtagtgcagg ctcagtccga gcagcgcggt tccggcgccg ccggcgcgcg cgacgggcag
     1981 gccaagaagt ccgccggcaa tggtgtgcag caccgcggtc ggtgcgacgc cgcgatagca
     2041 gaaccagaac gtcagcgcat aggcgatgtc gagcgtggcg gcgatgccgc cgccgacccc
     2101 gatggcgaaa acggcgggtc gtcgcgtcat cgggctttgc tcgccgattg cgcaggctgc
     2161 gcgctggagt cgtgcaccgc atcgatcagc acgcgcaggt gttccggatc gacgtcgggc
     2221 gtgatgccgt ggccaaggtt gaagacatgg ccgggatgcg ggccgaagct gtcgagcgtc
     2281 ttctttactt cggcacggat ggtttcgggt tgcgcatgca gcacggccgg atcgaggttg
     2341 ccctgcagcg cgacgcggtt gccgacgcgg tggcgcgcat catcgagcgc gatcgtccag
     2401 tccaggccaa gcgccgcaca gccggtatcg gcgagcgttt ccagatgcgc gttcgcgcct
     2461 ttgctgaaca gaatcaacgg aatggacttt gcagcattgt ggatttgtag actttgcgcg
     2521 atctgcgcga gatagcgcag gctgaactcg cggaacatgt ccggcgcgag caggccgccc
     2581 caggtgtcga ataccatcag cgcctgtgcg ccggcggcgg cttgcgcggc gaggtaggcg
     2641 gcgacggaac gcgcgacgac gtcgagcaac ttgtgcatcg tcgcggaatc gtcgagggcc
     2701 agggccttgg ccttggcgaa gctggacgat cctccgcctt cgatcatgta acaggccagc
     2761 gtccacggac tgccggcgaa cccgatcagc ggtaccttgt cgtgcagttc gcgccggatc
     2821 aggcgcaccg cgtccatcac gtaacgcagg tcggtttcgg g
//
LOCUS       DASRVO010000651         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_71257, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000651 DASRVO010000000
VERSION     DASRVO010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71257"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VFF05_08015"
     CDS             <1..384
                     /locus_tag="VFF05_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZW51791.1"
                     /translation="GALAQRLTDPRYKMNKAYFVQVEGDPTDAQIVQLCKGVHLNDGL
                     TLPAQARRLYAAPDWLWPRDPPVRFRKTVPDAWIELTICEGRNRQVRRMTAAVGLPTL
                     RLIRVRVGDAVLDGLAPGQTRILQP"
     gene            381..>1243
                     /locus_tag="VFF05_08020"
     CDS             381..>1243
                     /locus_tag="VFF05_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZW51792.1"
                     /translation="MTRDWRTPLEILLLGAIWGSSFLFMRIAAPHFGPLPLVEVRVML
                     GALLLSPFLWRARKQLDAAHWRRLAIVGVLNSAIPFSLFAWGAERAPAGIGAIANSMT
                     VLFTALIAFAFYREHIGLRRGIALVAGFAGVVILASGRTAGDNIMLAAIAGTLASFCY
                     GIAVNLTKRKLGDLQPVVGVAGTLSCSALMLAPLAVATWPADPVPASSWLAAIALGAL
                     CTGFAYFLFFRLIQRISATRAATCNYLIPVFGVGWAWLLLGETPTPTMLVSGALILGS
                     VIFSQREPARRA"
BASE COUNT          168 a          423 c          411 g          241 t
ORIGIN      
        1 ggtgcgctgg cgcaacgcct gaccgatcct cgttataaga tgaacaaggc ctatttcgtg
       61 caggtggaag gcgatccgac ggatgcgcaa attgtacaat tgtgcaaagg cgtacatttg
      121 aacgacggtc tgacgctgcc cgcgcaagcg cggcgtctgt atgcggcgcc ggactggctg
      181 tggccgcgcg atccgccggt gcgttttcgc aagaccgtgc ccgacgcctg gatcgaactc
      241 accatctgcg aaggccgcaa ccgccaggtg cggcgcatga cggcagcggt cggattgccg
      301 acgctgcgcc tgatccgcgt gcgtgtcggc gacgcagtgc tggacggcct tgctcccgga
      361 caaacgagga ttctgcaacc atgacccgcg actggcgcac gccgctggaa atcctcctgc
      421 tcggcgcgat ctggggcagc tcgttcctgt tcatgcgcat tgccgcgccg catttcggcc
      481 cgctgccgct ggtcgaggtg cgcgtgatgc ttggcgcact gctgctttcg ccgttcctgt
      541 ggcgcgcgcg caagcagctc gacgccgcgc actggcgccg gcttgcgatc gtcggcgtgc
      601 tcaactccgc gattccgttt tccctgttcg cctggggcgc ggagcgcgcg ccggccggca
      661 ttggcgcgat cgcgaacagc atgaccgtgc tgttcacggc gctgatcgcg ttcgcgttct
      721 atcgcgagca cattggcctg cgccgcggca tcgcgctggt cgccggattc gcgggtgtcg
      781 tcatcctggc gagtggacgc accgccggcg acaacatcat gctggcggcg atcgccggca
      841 cgctggcctc gttttgctac ggcatcgccg tcaacctgac caagcgcaag ctcggcgacc
      901 tgcaaccggt cgtcggcgtc gccggcacgt tgagttgttc ggcgctgatg cttgcgccac
      961 tggccgtcgc gacctggccg gccgatccgg tcccggcgtc ctcatggctt gccgccatcg
     1021 cgctcggcgc gttgtgcacg gggttcgcgt acttcctgtt ctttcgcctg atccagcgca
     1081 tctccgccac gcgcgcggcg acctgcaact acctgatccc ggtattcggc gtcggctggg
     1141 catggctgct gctcggcgaa acgccgacgc caaccatgct cgtttccggc gcgttgatcc
     1201 tgggcagcgt gattttcagc cagcgcgaac cggcgcgccg ggc
//
LOCUS       DASRVO010000652         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_305788, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000652 DASRVO010000000
VERSION     DASRVO010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305788"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VFF05_08025"
     CDS             complement(<1..532)
                     /locus_tag="VFF05_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK family protein methyltransferase"
                     /protein_id="HZW51793.1"
                     /translation="MTSELSTIIDLIRYGASRFAGAGLTFGHSYDNALDEATHLVLHT
                     LHLPHDLSPAYGSAKLTAAEKKAVLGLIERRVKEHKPVAYLTGEAWFAGLKFKSDERA
                     LVPRSPIAETIQNGFSPWLDGTVVHRALDLCTGSGCIGIAMAAHNPDWRVDLVDISDK
                     ALALARENIAFQHVEDR"
     gene            595..1233
                     /locus_tag="VFF05_08030"
     CDS             595..1233
                     /locus_tag="VFF05_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZW51794.1"
                     /translation="MNTSRSTSSIPIWLFVAAAVAVALGLWLGQRFFAAPEQPKLQNA
                     VLYPAPRAIPEFHLTQADGKPLTLADWRGYWTVVYFGYTSCPDVCPTTLTTFKQAWKD
                     LAARGLTDKLRFDFISVDPQRDTPDVLHKYVSYFDPAFVAATGIDDELTPLTRAMGLV
                     YSRSTDANGKIEVDHSAAAVIVDPQGREAGIFRPPFVANAIAHDLAALAASH"
     gene            1236..>2049
                     /gene="asd"
                     /locus_tag="VFF05_08035"
     CDS             1236..>2049
                     /gene="asd"
                     /locus_tag="VFF05_08035"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460408.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HZW51795.1"
                     /translation="MRLSVLAQYALPHRALSRLVYWATRWTWRPWKNFLIGRVVRAYA
                     VEMSEAEQPDPFAFPTFNAFFTRALNPAARPMPDDPRAIICPADGRVSQLGSIRDGRI
                     FQAKDQDFSAAELLGDEAAAAAYANGRFITVYLSPRDYHRVHAPLTGRLRETLHIPGR
                     LFSVAPFAVEAVPRLFARNERLVCHFDGAHGPFAVVMVGAMLVSGVETVWSGMEIPPY
                     ASAVVRKDWRGKDIRLERGAELGRFNMGSTAIVLLPSGAAEFDPELSVQIPVR"
BASE COUNT          347 a          695 c          625 g          382 t
ORIGIN      
        1 cgcgatcttc gacgtgctgg aacgcgatgt tctcgcgcgc cagcgccagc gccttgtcgc
       61 tgatgtcgac aaggtccacg cgccaatccg gattgtgcgc ggccatggcg atgccgatgc
      121 agccggagcc cgtgcacaaa tccagcgcgc gatgcacgac cgtgccgtcg agccacggcg
      181 agaacccgtt ctggatcgtc tcggcgatcg gcgaacgcgg caccagcgcg cgttcgtcgg
      241 acttgaattt caggcccgcg aaccacgcct caccggtgag atacgcgacc ggtttgtgct
      301 ccttcacgcg gcgctcgatg agcccaagca ccgccttttt ctcggcggcg gtcagtttgg
      361 ccgaaccata cgccggcgac aggtcgtgcg gcaggtgcag cgtgtgcagg accagatgcg
      421 tggcctcgtc cagcgcattg tcgtagctgt ggccgaacgt gagcccggcg cctgcgaaac
      481 ggctggcgcc gtaacggatg aggtcgatga tggtggagag ttcggatgtc acgagcagat
      541 tctgttccag gcaaggcagc cagtataggt gaagccgccc gctataatgc gcggatgaat
      601 acttcacgca gcacttcctc gattccaatc tggctcttcg tcgccgccgc cgtggccgtc
      661 gcgctcggcc tgtggctggg ccagcgtttc ttcgccgctc cggagcaacc caaactccag
      721 aacgccgtgc tgtatccggc gccgcgcgcg ataccggaat tccaccttac ccaggccgac
      781 ggcaagccgc tgacccttgc cgactggcgc ggctactgga ccgtcgtgta cttcggctac
      841 accagttgcc cggatgtgtg cccgaccacg ctcacgacct tcaagcaggc gtggaaggac
      901 cttgccgcgc gcggtctcac cgacaagctg cgcttcgatt tcatctcggt cgatccgcag
      961 cgcgacacac ccgacgtgct gcacaaatac gtgagctatt tcgatcccgc ttttgtcgcc
     1021 gccaccggaa tcgacgacga gctcaccccg cttacccggg cgatgggcct ggtctattcg
     1081 cgctcgaccg atgcgaacgg caagatcgaa gtcgaccaca gtgccgccgc cgtaatcgtc
     1141 gatccgcagg ggcgcgaagc cggtattttc cgcccgccgt tcgtcgcgaa tgcgatcgcg
     1201 cacgatctcg ccgcgctcgc tgccagtcac tgaacatgcg tctttccgta ctcgcgcagt
     1261 acgcactgcc gcatcgcgcg ctgtcgcgcc tggtgtactg ggcgacgcgc tggacctggc
     1321 gtccatggaa gaacttcctg atcggccggg tggtgagggc ctacgccgtc gaaatgagcg
     1381 aggccgagca acccgatccg ttcgcctttc cgacgttcaa tgcgttcttc acgcgcgcgc
     1441 tcaaccccgc tgcgcggccc atgcccgatg atccccgcgc catcatttgt cctgccgatg
     1501 gccgcgtcag ccaactcgga tcgattcgag atggaagaat tttccaggcc aaggaccagg
     1561 atttttccgc cgccgaactg ctcggcgatg aagctgccgc agcggcatac gcgaatggcc
     1621 gcttcatcac cgtctacctc tcgccacgcg actatcaccg cgtgcatgcg ccactgaccg
     1681 gacgcttgcg cgaaaccctg cacattcccg ggcgcctgtt cagcgtcgcg ccgttcgccg
     1741 tcgaggcggt gccgcgcctg ttcgcacgca acgaacgcct ggtctgccac ttcgacgggg
     1801 cgcatggacc tttcgccgtc gtcatggtcg gtgccatgct cgtgtcgggc gtggaaaccg
     1861 tctggagcgg catggaaatc ccgccctatg ccagtgcggt cgtgcgcaag gactggcgcg
     1921 gcaaggacat ccgtctcgaa cgcggcgcgg aactcggacg cttcaacatg gggtccacgg
     1981 cgatcgtttt gctgccatcc ggggcggcgg aattcgaccc tgaattgtcc gtacaaatac
     2041 cggtcagaa
//
LOCUS       DASRVO010000653         1746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_148172, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000653 DASRVO010000000
VERSION     DASRVO010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148172"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VFF05_08040"
     CDS             complement(<1..632)
                     /locus_tag="VFF05_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW51796.1"
                     /translation="MMKKVLAAQYPVLRGWAWLTVANRASSTLTVVAIFALGASLNGK
                     GEVSVGGIVSFVGFAMMLIGRLEQFASFISGLFFQTHSLSDFFAVLDTKEVLQEKPGA
                     PALPAVRGDVVFDNVSFGYEVTHPALKHLSFSAKAGSTIALVGPTGAGKTTALSLLYR
                     AYDPSEGRITIDGIDIREVSLNSLREHIAVVFQDPGLFYRSIRDNLLIGKP"
     gene            complement(651..1301)
                     /locus_tag="VFF05_08045"
     CDS             complement(651..1301)
                     /locus_tag="VFF05_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HZW51797.1"
                     /translation="MPLLRLYLRVIALLAPEKWLAIALAVANLALAGVFFLEPWLFGR
                     VVDALAAKHDAWHYIGWWAGAGLGGVIASVWVSLYADRLAHRRRLVAIAMFFEHAVAL
                     PLSFHGQHHTGRLLRIMHGGTGNLFGLWLGFFREHLATLLAIVVMVPLALKMNWKLAL
                     LMIALMVSFAVFNSIAMRRTLKAQGEVEELHHEITERAGDVFGNVLVVQSFTRLSA"
     gene            1359..>1746
                     /locus_tag="VFF05_08050"
     CDS             1359..>1746
                     /locus_tag="VFF05_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HZW51798.1"
                     /translation="MTASLDIAIERAVARVALNRPQVHNAFDDVLVADLTAALGRLDR
                     DPGVRFVVLTGNGSTFSAGADLNWMRGMARANEAENRNDSERLAALMRTLNTLAKPTL
                     ARVNGSAYGGGVGLVACCDIAIAADSA"
BASE COUNT          339 a          581 c          547 g          279 t
ORIGIN      
        1 ggtttgccga tcagcaggtt gtcgcggatc gagcggtaga aaagtcccgg atcctggaac
       61 accacggcaa tgtgctcgcg cagcgaattc aaactcacct cgcggatgtc gatgccgtcg
      121 atcgtgatcc tgccctcgct cggatcgtag gcgcgataga gcaggctcaa tgccgttgtc
      181 ttgccggcgc cggtcggccc gaccagggcg atggtgctgc ccgccttcgc gctgaagctc
      241 aagtgcttga gcgccggatg cgtgacttcg tagccgaacg agacgttgtc gaacacgacg
      301 tcacccctga ccgcgggcag tgccggcgcg ccgggttttt cctgcagcac ttccttggtg
      361 tccagtacgg cgaaaaaatc gctgagcgaa tgggtctgga agaacaggcc cgagatgaaa
      421 ctcgcgaatt gttcgagccg cccgatcagc atcatcgcga aaccgacgaa gctgacgatg
      481 ccgccgacac tgacttcgcc cttgccgttg agcgaggcgc cgagcgcgaa gatcgccacc
      541 accgtcagcg tgctcgatgc gcggttggcc acggtcagcc aagcccagcc acgcagcacc
      601 ggatactgtg cggcgagcac tttcttcatc atgtcgcgga tcgcgttgac ctaggcggac
      661 aggcgcgtga agctctgtac caccagcacg ttgccgaaca cgtcgccggc gcgttccgtg
      721 atctcgtggt gcagttcctc gacttcgccc tgcgccttca gcgtgcgccg catggcgatg
      781 gagttgaaca cggcgaagct gaccatcagc gcgatcatca gcaacgccag cttccagttc
      841 atcttcaatg ccagcggcac catcacgacg atggcaagca gcgtcgccag gtgctcgcgg
      901 aagaatccga gccacaagcc gaacaggttg ccggtgccgc cgtgcatgat gcgcagcagg
      961 cgtccggtgt gatgctgtcc gtggaacgac agcggcaagg cgaccgcgtg ttcgaagaac
     1021 atggcgatgg ccacgagccg gcgccggtgc gccaaacggt cggcatacag cgatacccaa
     1081 acgctggcga tcacgccgcc caatccggca ccggcccacc agccgatgta atgccaggca
     1141 tcgtgcttcg ccgccagcgc atcgacgacg cggccgaaca gccaaggctc caggaagaac
     1201 acgccggcca gcgccaggtt cgcgacggca agcgcgatgg ccagccattt ttccggggcc
     1261 agcaaggcaa tcacacgaag atagaggcgc agcagcggca tgccgatagt ttagcaaccc
     1321 gctatcatcc gtatctcgct gagccggcag gatcgtgcat gaccgcatcg ctcgacatcg
     1381 ccatcgaacg cgccgtggca cgcgtggcgc tgaaccgccc gcaggtacac aacgccttcg
     1441 acgatgttct cgtcgcggat ctcaccgcag cgctcggcag gctcgaccgc gaccccggcg
     1501 tgcgattcgt cgtgctcacc ggcaatggca gcacgttctc cgccggcgcg gacctgaact
     1561 ggatgcgcgg catggcgcgc gcgaacgagg cggagaaccg caacgactcc gaacgtctcg
     1621 ccgcgctgat gcgcacgctc aatacgctgg ccaagccgac gctcgcgcgc gtcaacggat
     1681 cggcctatgg cggcggcgtg ggtctggtcg cctgctgcga catcgccatt gccgccgaca
     1741 gcgcga
//
LOCUS       DASRVO010000654         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_355989, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000654 DASRVO010000000
VERSION     DASRVO010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355989"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFF05_08055"
     CDS             <1..226
                     /locus_tag="VFF05_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW51799.1"
                     /translation="YVMQTLALLAPGDIGQGIAKSAVFAVLITLIGVSTGFSVQGGAE
                     GVGRATTRAVVLSICWIIVVDMIFSFFLNR"
     gene            235..1038
                     /locus_tag="VFF05_08060"
     CDS             235..1038
                     /locus_tag="VFF05_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW51800.1"
                     /translation="MTTATAPLIEVEQLEAWYGRRRILAGVDFSVSAGEIRVIMGGSG
                     SGKSTLLRHLLGLHKPRGGRIRLLGVDIGRASEREMLDVRRQIGVSFQGGALLTSLSV
                     GGNVALPLREHTNLDEATIRIMSRLKLEVVNLGGFQDLMPAQLSGGMVKRAALARAIA
                     MDPKLLFFDEPSAGLDPVVSAELDELILRLRDALRVTIVVVTHELESAFKIADTITVL
                     DQGKILMTGTVDEVRHCDNERIQDLLNRRPREIQVDADAYLKRLTEEAA"
     gene            1049..1975
                     /locus_tag="VFF05_08065"
     CDS             1049..1975
                     /locus_tag="VFF05_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HZW51801.1"
                     /translation="MKRDSINYVLVGAAVLVAGVLLLVALAMITGRGGATTAYFTHYR
                     NVTGLRYGAPVFYQGYRIGQVGAIVPERSPDGTRYKIELDVRRDWPIPKDSVAHLTAT
                     GLLADVAVGIGEGKDRAMAAPGAELAGAENADIFSAMNELAGQITQLTRGQISPLIQT
                     LSKRVDSITGAIDKGTPDIVEQSRKLLTQLNAASASLNDVLKPDNRAALGAILANTQQ
                     MTAQLHDTQQKLDDALGQLDGIARENRPGIRDSVADLRAVLEALSGRIDSISQHLQTA
                     SRNFDEFSREVRKNPNRILISPKPDKVEEQEP"
     gene            1972..2586
                     /locus_tag="VFF05_08070"
     CDS             1972..2586
                     /locus_tag="VFF05_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HZW51802.1"
                     /translation="MINPRTILLALGLAALAACSVDPVPDVTYFRLPPPAALPHADKP
                     LSALPIEVETFRGEGVYAEQALIYATTAQADALRAFHYQLWNDPPSRALQARLTGMLR
                     DSGISSLVTDRLPASTPALRVHGRILRYERVPRDGGFVVAVALEMRVDQDQGEPLLER
                     SYAAQIPTTDSSMTATVDAFGRAVDKNFAAFFADLAALGSGHAD"
     gene            2576..2911
                     /locus_tag="VFF05_08075"
     CDS             2576..2911
                     /locus_tag="VFF05_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51803.1"
                     /translation="MPTEAQRSAMLAAMGIDVYRLRASPATAVPLRVGVDARTCVCVD
                     GSDDASVERLLAMLPAVLGIARERVSRDIVADDGAIVVDASALRANGAAKRVLWQSLK
                     PLARRLREN"
BASE COUNT          515 a         1012 c         1000 g          525 t
ORIGIN      
        1 ctacgtgatg caaaccctcg cgctgctggc gccgggcgac atcgggcagg gcattgccaa
       61 gtccgcggta ttcgccgtgc tgatcaccct gatcggcgtg agcacgggat tctcggtgca
      121 gggcggtgcc gagggcgtgg gtcgcgcgac cacgcgcgcg gtggtgctgt cgatctgctg
      181 gatcatcgtg gtcgacatga tcttctcgtt tttcctgaat cgctgattcg atccatgacg
      241 accgcgactg cgccgctgat cgaagtcgag caactcgaag cctggtacgg gcggcggcgg
      301 attctggctg gcgtggattt ctcggtgagc gccggggaaa tccgcgtcat catgggcggc
      361 tcgggttcgg gcaaaagtac acttttgcgc catctgctcg gcctgcacaa gccgcgcggc
      421 gggcgcatcc gcctgctcgg cgtggacatc ggccgcgcca gcgagcgcga gatgctcgac
      481 gtgcgccgcc agatcggtgt gtcgttccag ggcggcgcgc tgctcacttc gttgtcggtc
      541 ggcggcaacg tcgcgctgcc gttgcgcgaa cacacgaacc tggacgaagc cacgatccgc
      601 atcatgtcgc gcctcaagct cgaagtggtg aacctgggcg gattccagga cctgatgccg
      661 gcacagctct ccggcggcat ggtcaagcgc gcggcgctgg cgcgcgcgat cgcgatggat
      721 cccaagctgc tgttcttcga cgaaccctcg gccggcctgg atcctgtcgt gtcggccgaa
      781 ctcgacgagt tgatcctgcg cctgcgcgac gcgctgcgcg tgacgattgt ggttgtcacc
      841 cacgaactgg aaagcgcgtt caagattgcc gataccatta ccgtgctcga tcagggcaaa
      901 atcctcatga ccggcacggt ggatgaggtg cgccattgcg acaacgagcg catccaggac
      961 ctgctcaatc ggcggccgcg cgagatccag gtcgacgcgg acgcctacct gaaacgcctg
     1021 acagaagaag ccgcatgagc atttcaacat gaaacgcgac agcatcaact acgtgctggt
     1081 cggcgctgcg gtgcttgtcg ccggagtgct gttgttggtg gcgttggcga tgatcaccgg
     1141 ccgcggcggc gcgacgacgg cctacttcac gcattaccgc aacgtcaccg gactgcgcta
     1201 cggcgcgccc gtgttctatc agggttatcg tattggccag gtcggcgcga tcgtgcccga
     1261 gcgcagcccg gacggcacgc gttacaagat cgaactcgac gtgcgccgcg actggccgat
     1321 tccgaaggat tcggtcgcgc acctgaccgc caccggcctg ctcgccgatg tcgccgtcgg
     1381 catcggcgaa ggcaaggatc gtgcgatggc cgcgcccggc gcggaactcg ccggtgccga
     1441 gaacgccgac atcttcagcg cgatgaacga actcgccggc cagatcaccc agctcacacg
     1501 cggtcagatt tcgccgctga tccagaccct gtccaagcgc gtggactcga tcaccggcgc
     1561 gatcgacaag ggcacgccgg acatcgtcga gcagtcgcgc aaattgctca cccagttgaa
     1621 cgcggcgtcg gcctcgctca acgacgtgct caagccggat aaccgcgccg cgctcggtgc
     1681 gatcctggcc aacacccagc agatgaccgc gcaactgcac gacacgcagc aaaagctcga
     1741 cgatgcgctc ggccaactcg acggcatcgc gcgtgagaat cggcccggca tccgcgattc
     1801 ggtagccgac ctgcgcgccg tgctcgaggc gctgtccggg cgcatcgatt cgatcagcca
     1861 gcacctgcaa acggccagcc gcaacttcga cgagttctcg cgcgaggtgc gcaaaaatcc
     1921 gaaccggatc ttgatttcac caaaacccga caaggtcgag gaacaagagc catgatcaat
     1981 ccgagaacga tcctgctggc cctcggcctt gccgcgcttg ccgcctgctc cgtggatccg
     2041 gtgccggacg tgacgtattt ccgcctgccg ccgccggcgg cactgccaca cgcggacaag
     2101 ccgctgtcgg ccctgcccat cgaagtcgag acgtttcgcg gcgaaggcgt ctacgccgag
     2161 caggcgctga tctacgcaac cacggcgcag gccgatgcgc tgcgcgcctt ccactaccag
     2221 ctgtggaacg atccgccctc gcgcgcgttg caggcacgcc tgaccggcat gttgcgcgat
     2281 tcgggtattt cctcgctggt taccgaccgg ctgccggcga gtacgccggc gctgcgcgtg
     2341 cacggccgca tcctgcgtta cgagcgcgtg ccgcgcgatg gcggattcgt cgtggccgtg
     2401 gcactggaga tgcgcgtgga ccaggaccag ggcgagccgc tgcttgagcg cagctacgcc
     2461 gcacagattc cgaccaccga ctccagcatg accgcgacgg tggacgcatt tggccgcgcc
     2521 gtcgacaaga atttcgcggc gttctttgcg gaccttgccg cgctcgggag cgggcatgcc
     2581 gactgaggca caacgcagcg cgatgctcgc ggcgatgggc atcgacgtct accgtctgcg
     2641 cgcttcccca gccaccgccg taccgttgcg cgtgggcgtc gatgctcgta cttgcgtgtg
     2701 cgtggacggc agcgacgacg cttccgtgga gcgcctgctc gcgatgttgc cggcggtgtt
     2761 gggcatcgca cgcgagcgcg tgtcgcgcga cattgttgcc gatgacggcg ccatcgtcgt
     2821 ggatgcgtcc gcgctgcgcg caaacggtgc cgccaagcgc gtgctgtggc aatccttgaa
     2881 gccgctggcg cggcgtttgc gcgaaaactg acatggtcgc catcctcaag gaaccgtatc
     2941 cggcgctgcg cacgatgacc gcggacgatc tggagcaggt tgccgcgatc gaactcgcgg
     3001 cctacgagta tccgtggacg catggcatct tccgcgattg cctgcgcgcc gg
//
LOCUS       DASRVO010000655         4242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_19359, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000655 DASRVO010000000
VERSION     DASRVO010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19359"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFF05_08080"
     CDS             <1..271
                     /locus_tag="VFF05_08080"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4381 family protein"
                     /protein_id="HZW51804.1"
                     /translation="ALAAALSLFLRRLALRDAHAAAYAGERWLEYLDACSGGEDFRRG
                     VGRVLIDAPFRVHVDYDSIALIAVVRRFTRSVLASRAPAAGAAHA"
     gene            264..1274
                     /locus_tag="VFF05_08085"
     CDS             264..1274
                     /locus_tag="VFF05_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZW51805.1"
                     /translation="MLEFAWPWLFVLLPLPWLAARLLPPARDSAGAALRLPYALDGMD
                     AVTGAAPVASWRKWLALAGWVLLVCAAARPQWLGPPQDVARSGRDLLLAVDASGSMET
                     PDMQIGGRAVNRYAAVKAIAGDFIRRRVGDRVGLIVFGSQAYLLTPLTFDRDTVLKQL
                     DESAVGLPGRQTAIGDAVGLAVKRLQQRPRDQRVLILLTDGVNDAGVLDPYKAIDLAA
                     AEHVKIYTIGIGSDAMTIGGFFGPRVVNPSQDIDTRLLTDMAQKTGGKFYRAHDTAEL
                     AHIYREIDALEPAADKGEEFRPVDEWFWWPLSLAALIVLAHVLAGRALPLPRILRARF
                     AQ"
     gene            1271..3190
                     /locus_tag="VFF05_08090"
     CDS             1271..3190
                     /locus_tag="VFF05_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZW51806.1"
                     /translation="MNFSFHDFHFLRPWWLLTLAALPLLWRALSHGGADAGAWRGVVD
                     AHLLPHLLDLQATARSSRVPRWLATLAWIVACVALAGPAWERLPQPLFQNRAARVFAL
                     ELSPSMSAQDIKPSRYERARFKLDDMLAHSGGMQTALIAYAGDAFVVAPLTDDAHTVT
                     NLVDALDPSVMPSAGNDTGRAIDLGVKLVRQAGLRGGEIVVFADGADTDAVMAAQRAR
                     AAGIRVSVLGVGTDQGAPVALAQGGFLKDNTGNIVLPKLDAAALQAVAQAGGGRYASV
                     TADSGDIEALLAGAPMQSAADARAVDAVTTRFLDRGPWLVLLLLPLAALGFRRGWLML
                     LPLCLIAHTTPAQAFSWRDLWQRPDQQARAQLDAGHADKAQALARDPALRGAAAYRGG
                     DYAAAESDFARRDDAQAQYNRGNALAKQQDYKNAIAAYDDALRQDPQLADAQANKQAV
                     EDWLKQQQKQQQQNDKSGKNGQQDQSQKGGSQNEQSGGEQQDQQNKDAQNESGEQSGA
                     QHQDSKDGQQQNSGQDGKDKQSGADNTPQPNAQEQAKAQQEARATDEKFRQDMNRQLQ
                     QQGGARPGDKKGEPKPVRLGAREGDKAQNERDQAVEQWLARVPDDPGGLLRRKFRLEY
                     EIRRNGGHVPEAEGE"
     gene            3241..3969
                     /locus_tag="VFF05_08095"
     CDS             3241..3969
                     /locus_tag="VFF05_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HZW51807.1"
                     /translation="MCTAAQAASVRAWLDRSQMQLGETVTLNVQVEGDAQAQQPDFSP
                     LAGDFDMTGTQSSTSVNIVNGKTSSSVLWAVGLSPKHAGTFTIPALTVAGQQTRPLAL
                     VVSAPSAKATQPGGDVFVEAQVDPASPYVQQQVMLTVKLYFAVNLIDGNLEDPQVKGA
                     VVRKLGQDSRYRAQVGNRSYNVVERHYALTPEGNGELTLPPILFRGHAMDASGGGLFF
                     NQGRRLRARPLPATTPGCRRVRCR"
     gene            3960..>4242
                     /locus_tag="VFF05_08100"
     CDS             3960..>4242
                     /locus_tag="VFF05_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein BatD"
                     /protein_id="HZW51808.1"
                     /translation="MSLTATGIDATTRAQVGEPLTFTLRLEAQGLGFEQLPKLELPKI
                     EGADIYPDKPVTQDKNDGQWLYGVRERKFAIVPNRSGPLTLPPISVAWWD"
BASE COUNT          737 a         1368 c         1436 g          701 t
ORIGIN      
        1 cgccctcgcc gccgcactgt cgctgttcct gcgccgcctg gccctgcgcg atgcgcacgc
       61 tgcagcctat gccggcgaac gctggctgga atacctggat gcatgcagtg gcggtgagga
      121 tttccgtcgc ggtgtgggtc gcgtcctgat cgacgcgccg ttccgcgtac atgtcgatta
      181 cgacagcatt gcgctgatcg ccgtggtgcg gcgcttcacg cgcagcgtcc tcgcatcgcg
      241 tgcgcccgca gcgggagccg cgcatgcttg aattcgcctg gccatggctg ttcgtgttgc
      301 tcccattgcc ctggctggcg gcacgcctgc tgccgccggc gcgcgacagc gcgggtgcgg
      361 ccttacgttt gccgtatgcg ctcgatggca tggatgccgt gaccggcgcc gcgccggtcg
      421 cgtcttggcg caaatggctg gcgttggccg gttgggttct gctggtgtgc gccgcggcac
      481 gtccacaatg gctggggccg ccgcaggacg tggcgcgcag cgggcgcgat ctgctgctgg
      541 cggtggatgc atccggcagc atggaaacgc cggacatgca gattggcggc cgcgcggtga
      601 accgctatgc cgcggtcaag gccatcgctg gggacttcat ccgccgccgt gtcggcgatc
      661 gcgtcggcct gatcgtgttt ggcagccagg cgtacctgct cacaccgctg actttcgacc
      721 gcgacaccgt gctcaagcaa ctggacgaat ccgcagtcgg gctgccgggc cgccagaccg
      781 cgatcggcga tgcggtcggg ctcgcagtca agcgcctgca gcagcgcccg cgcgaccagc
      841 gcgtgctgat cttgctcacc gacggcgtca acgacgccgg cgtgctggat ccgtacaagg
      901 ccatcgacct ggccgccgcc gagcacgtga agatctacac catcggcatt ggctcggacg
      961 cgatgacgat cggtggattc ttcggtccac gcgtggtcaa tccgtcgcag gacatcgaca
     1021 cgcgtctgct caccgatatg gcgcaaaaaa ccggtggcaa gttctatcgc gcgcacgaca
     1081 cggcggagct tgcgcatatc tatcgcgaaa tcgatgcgct cgaacccgcc gcggacaaag
     1141 gcgaggaatt ccgtccggtc gatgagtggt tctggtggcc gctttcgctt gccgcgctga
     1201 tcgtactggc ccacgttctg gccggccgcg cgttgccgtt gccgcgaatc ttgcgcgcga
     1261 ggttcgcgca atgaacttct ccttccacga ttttcatttc ctgcggccat ggtggctgct
     1321 cacgctggcc gcattgccgc tgttgtggcg ggccttgtcg cacggcggtg cggatgccgg
     1381 cgcctggcgc ggcgtggtcg atgcgcattt gctgccgcat ctgctcgatt tgcaggccac
     1441 tgcgcgcagt tcgcgcgtgc cgcgctggct cgccacgtta gcgtggatcg tggcatgcgt
     1501 cgcgttggcc ggccccgcgt gggagcgcct gccgcagccg ctgttccaga atcgtgccgc
     1561 gcgcgtgttc gcgctggaat tgtcgccaag catgtccgcg caggacatca agcccagccg
     1621 ctacgagcgt gcgcgtttca agctcgacga tatgctcgcg cacagcggcg gcatgcagac
     1681 cgcgttgatc gcctatgccg gcgatgcctt cgtcgtcgcg ccgctgaccg acgatgccca
     1741 cacggtgacg aatctggtcg acgcgctcga tccgtcggtg atgccgtccg ccggcaacga
     1801 caccggtcgc gcgatcgacc ttggcgtgaa attggtccgg caggccggac tgcgcggcgg
     1861 tgaaatcgtg gtgtttgcgg acggcgccga cacggatgcc gtcatggcgg cgcagcgtgc
     1921 gcgcgccgcg ggcattcgcg tatccgtgct tggcgtcggc accgaccagg gcgcgccggt
     1981 cgcgctggcg cagggcggat tcctcaagga caacactggc aatatcgtgc ttcccaaact
     2041 cgatgcagcc gcgttgcaag ccgtcgcgca agccggtggc ggacgctacg ccagtgtcac
     2101 ggccgactcc ggcgatatcg aggccttgct cgccggtgcc ccaatgcagt cggcagccga
     2161 cgcgcgcgcc gtcgatgcgg tcacgacacg ctttctcgac cgcggcccgt ggctggtttt
     2221 gctgcttctg ccacttgctg cgctgggatt ccgccgcggc tggttgatgc tgttgccact
     2281 gtgtctcatc gcgcacacca caccggcgca ggccttctcg tggcgcgacc tgtggcagcg
     2341 gcccgaccag caggcgcgcg cgcagctcga tgccgggcat gcggacaagg cccaggcgct
     2401 ggcgcgcgat cccgccctgc gtggcgcggc ggcatatcgc ggtggcgact atgccgccgc
     2461 cgaatccgat ttcgcgcggc gcgacgatgc gcaagcacag tacaaccgcg gcaacgcgct
     2521 ggcgaaacag caggactaca agaacgccat tgccgcctac gacgatgcgc tacggcagga
     2581 tccacagctc gcggacgcgc aagcgaacaa acaggcagtc gaggattggc tgaagcagca
     2641 gcaaaagcag caacagcaaa acgacaagag cggcaagaac ggacagcagg accaatcgca
     2701 gaagggcggt tcgcagaacg agcaatccgg tggtgagcag caggatcagc agaacaagga
     2761 cgcccagaac gagtccggcg agcagtccgg cgcgcagcat caagattcca aagatgggca
     2821 gcagcaaaat tccggccagg acgggaagga caagcaatcc ggtgccgaca atacgcccca
     2881 gccgaatgcg caggagcagg ccaaggcgca gcaagaagcg cgtgcgaccg acgagaaatt
     2941 ccgccaggac atgaaccgcc agctgcagca gcagggcggt gccaggcctg gtgacaagaa
     3001 gggcgaaccc aagccggtgc gcctgggtgc gcgcgaaggc gataaggcgc agaacgagcg
     3061 cgatcaagcc gtcgagcagt ggctggcgcg cgtgccggac gatcccggcg gcctgctgcg
     3121 acgcaagttc cggctcgaat acgaaatacg gcgcaacggc ggacacgtac ccgaggcaga
     3181 aggcgaatga atccgcgcat ccgtaaattt gccgcattgt gtgcatttgc tgcattgtgg
     3241 atgtgtaccg ccgcgcaggc ggcgagtgtg cgtgcctggc tggaccgcag ccagatgcag
     3301 ttgggcgaga cggtcacgct gaatgtccag gtcgagggcg acgcgcaggc gcagcagccg
     3361 gatttcagtc cgctggcggg cgatttcgac atgaccggca cgcaaagttc cacttcggtg
     3421 aacatcgtca acggcaagac cagctccagc gtgctgtggg cggtgggttt gtcgccgaag
     3481 catgcgggca cattcacgat tccggcgttg accgtcgcag gccaacagac ccgaccgctc
     3541 gcgctcgtcg tgtctgcacc gtccgcgaaa gcaacgcaac cgggcggcga tgtgttcgtc
     3601 gaggcgcagg tcgatccggc gtcgccgtac gtgcagcagc aggttatgct gacggtgaaa
     3661 ctgtatttcg cggtaaacct catcgatggc aatctggagg atccgcaggt caagggcgcc
     3721 gtggtgcgca agctcggcca ggacagccgc taccgcgcgc aggttggcaa tcgcagttac
     3781 aacgtcgttg agcgccacta cgcgctcacg ccggagggca acggcgagct cacgcttccg
     3841 ccgatcctgt tccgcggaca tgcgatggac gcgagcggcg gcggattgtt cttcaaccag
     3901 ggccggcgat tgcgcgcccg accgcttccg gcaacgacac ctggctgccg gcgcgttcga
     3961 tgtcgctgac cgcgactggc atcgacgcca ccacgcgtgc gcaagtcggc gaaccgctga
     4021 cgttcacgct tcggctcgaa gcgcaaggat tggggttcga gcaactgccg aagcttgaat
     4081 tgccgaagat cgaaggcgcc gacatctacc ccgacaaacc cgtcacgcaa gacaagaacg
     4141 acgggcaatg gctgtacggc gtgcgcgagc gcaagttcgc gatcgtgccg aaccgatccg
     4201 gtccgctgac attgccgccg atcagtgtag cctggtggga ta
//
LOCUS       DASRVO010000656         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_46070, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000656 DASRVO010000000
VERSION     DASRVO010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46070"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..805
                     /locus_tag="VFF05_08105"
     CDS             68..805
                     /locus_tag="VFF05_08105"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZW51809.1"
                     /translation="MPKPRRVTDTRSARRKPPPRRAAADATTAASGKAARSRRTQARI
                     LDAAEELFSHHGIYGVSIRDIADRADVDTALLHYHFANKDGIYEAVLLRRAGEINAAR
                     MHALDDYALACGGKPDVEGIVRAYLHPVFERLRNGGRGFNNFAALIALANNSELWGGG
                     TVKEYFDSAVQRLIELLRLALPKAREVDLYWGYQFISGALTLSYSRNDRIEGLSRGRC
                     RANDYAAIEPRLVEWCTAGIRAIGARR"
     gene            954..>2391
                     /locus_tag="VFF05_08110"
     CDS             954..>2391
                     /locus_tag="VFF05_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZW51810.1"
                     /translation="MTCKPLKIAIAAALFASAAAHADAVSDAAAGSAADAPGAAAARK
                     DDAAKKNEQAASMETITVTARRRAELLQDVPIAVTALSAATLETQNVQNLNDLAGNVP
                     NLVIHDARGSNTTITAYIRGVGQSDPTWGSDPGVGIYLDDVYIARPQGALLDVFDVQR
                     IEVLRGPQGTLYGKNTIGGAIKYVSRDLPTQDEGYAQVDAGNEGRADLKVGLGGATDD
                     GQFRARVAYASLHHDGYGTNSNTGDSVSNQNTNAGRISAGFFPKDVPLDMQFSADTVH
                     DNSNPRGARMLAPNKFAPTYAPLDSDYDVRSGMPSLNPTHMVGESFKVNLYANDAWTF
                     KSITAHRQSNTLTNIDFDTTPLSLADVQAYYRDSQTSQEFQANFQGSGGTSGVVGLYY
                     FDGTAAGWIHNNFFNALFGVTGGKVDTRSYAVYTDWTWTPSDRWQLSAGARFTDEKKH
                     AIVKNYTTADATFGAPVAVTADFDRTRQP"
BASE COUNT          460 a          821 c          742 g          368 t
ORIGIN      
        1 ggctacttgg ttattgatca aaccgtgaat ttcgcgagaa tagcgcaacg actccggcga
       61 gatgcccatg cctaagccaa gacgcgttac cgacacccga tctgcccggc gcaagccgcc
      121 accgcgccgc gcggcggcgg atgccacgac ggccgcttcg ggcaaggccg cgcgcagccg
      181 ccggacgcag gcgcgcatcc tcgatgcggc cgaggaattg ttctcgcacc acggcatcta
      241 cggcgtgagc attcgcgaca tcgccgatcg cgccgacgtt gataccgcac tgctgcatta
      301 tcacttcgcc aacaaggacg gcatctacga agcggtgctg ctgcgccgcg cgggcgagat
      361 caacgcggcg cgcatgcacg cgctggacga ctacgccctc gcgtgcggcg gcaagcccga
      421 cgtcgaaggc atcgtgcgcg cttatctgca tccggtgttc gagcgcctgc gcaacggcgg
      481 tcgcggcttc aacaacttcg ccgcgctcat cgccctggcc aacaattcgg agttgtgggg
      541 cggcggtacg gtgaaggagt atttcgattc cgccgtgcag cgcctgatcg agctgttgcg
      601 gctggccttg ccgaaggcgc gcgaagtcga tttgtactgg ggctatcaat tcatttccgg
      661 ggcgctgacc ctgagctact cgcgcaacga tcgcatcgag gggctgtcgc gcggtcgctg
      721 ccgcgccaac gactacgcgg cgatcgaacc gcgcctggtc gagtggtgca cggccgggat
      781 ccgcgcgatc ggcgcgcgcc gctgagccaa gccaaatcgg gattgcgtcg tattgctgca
      841 ttgcggcttg cgtgccagaa aacaggctgt tagtttattc accaataaat aaaaatcaat
      901 ctgcaccgca caatgccgca cccgcacggc accaacatcc tggagagtcc acaatgacct
      961 gcaagcccct gaaaatcgcg attgccgccg ccttgttcgc cagtgcggcg gcgcacgccg
     1021 acgccgtgtc cgatgccgcc gccggctccg ccgcggacgc gcccggcgcg gcggctgcaa
     1081 ggaaggacga tgcggcgaag aagaacgagc aagctgcgtc gatggaaacc atcaccgtga
     1141 ccgcgcgccg tcgcgccgag ttgctgcagg acgtgccgat cgcggtaacg gcgctgtccg
     1201 ccgcgacgct cgagacgcag aacgtgcaga acctcaacga cctggccggc aacgtgccga
     1261 acctcgtgat ccacgacgcg cgcggcagca acaccacgat caccgcgtat atccgcggcg
     1321 tgggccagtc cgacccgacc tggggctcgg accccggcgt tggcatctac ctggacgacg
     1381 tctacatcgc gcggccgcag ggtgcgttgc tcgacgtgtt cgacgtgcag cgcatcgaag
     1441 tgctgcgcgg cccgcagggc acgctgtacg gcaagaacac gatcggcggc gcgatcaagt
     1501 acgtctcgcg cgacctgccc acgcaggacg aaggctatgc gcaggtcgat gccggcaacg
     1561 agggccgcgc cgacctgaaa gttggcctgg gcggcgccac ggacgacggc cagttccgtg
     1621 cgcgcgtggc ctatgcctcg ctgcaccacg acggctacgg cacgaactcc aacaccggcg
     1681 attcggtcag caaccagaac acgaatgccg ggcgcatatc cgcgggcttc ttccccaagg
     1741 acgtgccgtt ggacatgcag ttcagtgccg acaccgtgca cgacaattcc aatccgcgcg
     1801 gcgcacgcat gctcgcgccg aacaagttcg cgccgaccta cgcgccgctg gacagcgact
     1861 acgacgtgcg cagcggcatg cccagcctca accccaccca catggtcggc gaatcgttca
     1921 aggtgaacct gtacgcgaac gatgcgtgga cgttcaagtc gatcaccgcc catcgccagt
     1981 cgaacacgct gaccaacatc gatttcgaca ccacgccgtt gtcgcttgcc gacgtgcagg
     2041 cgtactaccg agatagccag acgtcgcagg aattccaggc caacttccag ggcagcggag
     2101 gcaccagcgg cgtcgtcggg ctctactatt tcgacggtac cgcggccggc tggatccaca
     2161 acaatttctt caacgcgctg ttcggcgtga ccggcggcaa ggtggatacg cgcagttacg
     2221 ccgtttacac cgactggacc tggacaccct ccgaccgctg gcaattgtcc gccggcgcgc
     2281 gcttcaccga cgagaagaag cacgccatcg tcaagaacta caccacggcg gacgccacct
     2341 ttggcgcgcc ggtcgcggtc accgccgact tcgaccgcac gcgccaaccg a
//
LOCUS       DASRVO010000657         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_252912, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000657 DASRVO010000000
VERSION     DASRVO010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252912"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VFF05_08115"
     CDS             complement(<1..1130)
                     /locus_tag="VFF05_08115"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HZW51811.1"
                     /translation="MTKTTATCLAALVLAAASTAGQASARTASLGEAIYRDTSLSSSG
                     KMACSTCHDPAKAHAQDNDLPVQLGGAELATPGFRAVPSLRYMDPNLPFIFANDGTPI
                     GGFTRDGGARDLVEQARTPILAAHEMDNADAAGFAARLSVAAYADDFRSVFGADVFAD
                     PDRTLLAAEYSLSEYQNTAREFHPFDSKFDLFLQRKVMLSPQELRGFALFNRKDKGNC
                     AGCHPSTPANGQPPMFTDFTYDNLGVPRNPQIPANADPDWFDMGLCGPFRTDLAARTD
                     LCGQFKVPTLRNVATRRVFFHNGYFHDLKSALEFYVQRDTNPEKWYPLDAQGKPQKFD
                     DIPALFARNVNRSEAPYNRKPGQAPALSNAEIDDVVAFLKTLT"
BASE COUNT          196 a          353 c          395 g          205 t
ORIGIN      
        1 gtcagggtct tcaggaacgc gaccacatcg tcgatttccg cattcgacag tgccggcgcc
       61 tggccgggct tgcggttgta cggcgcttcg gacctgttca cgttgcgcgc gaacagcgcc
      121 gggatgtcgt cgaatttttg cggcttgccc tgcgcgtcca gcggatacca cttctccgga
      181 ttggtatcgc gctgcacgta gaactcgagc gcggatttca ggtcgtggaa atagccgttg
      241 tggaagaaca cgcggcgcgt ggcgacgttg cgcaacgtcg gcaccttgaa ctggccgcac
      301 aggtcggtgc gcgccgcgag gtcggtgcgg aacggtccgc acaggcccat gtcgaaccag
      361 tccggatcgg cgttcgcggg aatctgcgga ttgcgcggca cgccgaggtt gtcgtaggtg
      421 aagtcggtga acatcggcgg ctggccgttg gccggcgtac tcgggtggca gcccgcgcaa
      481 ttgcccttgt ccttgcggtt gaacagcgcg aaaccgcgca actcctgtgg cgagagcatg
      541 accttgcgct gcaggaacag atcgaacttg gaatcgaacg gatggaattc gcgcgccgtg
      601 ttctggtatt cggaaagcga atactcggca gcaagtagag tccggtccgg gtccgcgaac
      661 acgtcggcgc cgaacacact gcggaaatcg tccgcgtaag cggcgacgct caggcgcgcg
      721 gcaaatccgg ccgcatccgc gttgtccatt tcatgcgccg ccaggatcgg cgtacgcgcc
      781 tgctcgacca ggtcgcgcgc accgccgtcg cgcgtgaagc caccgatcgg cgtgccgtcg
      841 ttcgcgaaga tgaacggcag attcggatcc atgtagcgca gcgacggcac cgcgcggaat
      901 cccggtgtgg ccagctccgc cccgcccagc tgcacgggaa gatcgttgtc ctgcgcgtgc
      961 gccttcgccg gatcgtggca agtcgagcag gccatctttc cggaggacga caaggacgtg
     1021 tcgcggtaga tggcctcgcc cagactggcc gtcctcgcag acgcctgccc ggccgtggag
     1081 gcggctgcga ggacgagagc tgcaaggcag gtggcagtcg ttttggtcac gtcggcgagg
     1141 catcactta
//
LOCUS       DASRVO010000658         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_71554, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000658 DASRVO010000000
VERSION     DASRVO010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71554"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFF05_08120"
     CDS             complement(<1..242)
                     /locus_tag="VFF05_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51812.1"
                     /translation="MTDDRRIAAIAAELIALNDSGDTVAPFSERYPGLDASAGYRAAA
                     RLHAARLARGWNLVGRKIGFTNRTIWPRYGVYEPMWG"
     gene            329..754
                     /locus_tag="VFF05_08125"
     CDS             329..754
                     /locus_tag="VFF05_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZW51813.1"
                     /translation="MEHYLVWLLIGMTLVIVELLTGTFYLLVLGIACFGGSASAFFGH
                     TFGTQAIVAGCVAALGVVLVHAYRARNAARQMAPIDAGMPANFESWIDKSAGLARVRY
                     RGAAWDARIEESASLEPGSLVYVVAANGNTLKVTVKRPA"
     gene            798..1793
                     /locus_tag="VFF05_08130"
     CDS             798..1793
                     /locus_tag="VFF05_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HZW51814.1"
                     /translation="MLIKLVIALAISIAAAFVPATAPYAVWIFLIAVVVAFVIEGVRI
                     VPQQHAFVIERLGRFHAILEPGLNLIIPFLDRVAYTHNLKEVPLDVPEQICITRDNTQ
                     LGVDGVLYYQVTDPRLASYGSSDYITAIVQLAQTTLRSEIGKMELDKTFESREEINRQ
                     IVAALDEAGRNWGIKVLRYEIKSLTPPESILRSMQQQITAEREKRALIAKSEGERQQE
                     INIADGKRQAAVLESEGEKQGAINRAQGEATALRLIADANAAAVRAVAEAIADKGGMD
                     AANLKVAQEYVAAFANLAKQSNTLIIPANTGDVAAFVGSAMTVLDKTRQAAAQRT"
     gene            1861..2064
                     /locus_tag="VFF05_08135"
     CDS             1861..2064
                     /locus_tag="VFF05_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51815.1"
                     /translation="MLGIALFVAAFIFASVLLAIAATAALLIWGWLWWRTREIRRAAR
                     EGDNVVIEGEYRVERESRRLEDD"
     gene            complement(2061..>3053)
                     /locus_tag="VFF05_08140"
     CDS             complement(2061..>3053)
                     /locus_tag="VFF05_08140"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HZW51816.1"
                     /translation="VFFAVHFEAKRYNLAGVPKAELPRFISVMVDRGHLFVPILMVLF
                     GLMAGYSAPLCALVGALTCLPMALLRKATREGIGWRSVIDALEDGAKNTLAVAMACAC
                     AGVVIACVTITGLGIVFTQMVVALAQNSLVLALILTALAGIVLGMGMPTTPAYIMMVA
                     LLVPAVIKLGATTPTAHMFAFYFAILSAITPPVALAVFAAAGLAKSNMWKSGFAAMRV
                     AAPAYIVPFMFVYEPTLLLIVKDWSTQWEYVALSVVTATMGAICLAASLHGWLLAFAA
                     LWQRTALFIAAMLLIKPGLYSDLAGLALLAAVVTAQLMERRRAAGRPQLARPGS"
BASE COUNT          584 a          937 c         1012 g          520 t
ORIGIN      
        1 ccccacatcg gctcgtacac gccgtagcgt ggccatatgg tgcggttggt gaaaccgatt
       61 ttgcgtccga ccaggttcca gccgcgcgcg agacgcgccg cgtgcagccg cgcggcggca
      121 cgatagccgg cggatgcatc gagtccggga tatcgttcgc tgaagggtgc aacggtatcg
      181 cccgagtcgt tcagcgcgat cagttcggcg gcgattgcgg cgatgcgtcg atcgtcggtc
      241 atgcttccgt cttctttgca acgcagattg taccgtgcga aaaaagcgcg gttgcggttt
      301 ggtcatgcac gtgcgaaatt agcgcgccat ggagcactac ctcgtttggc tgctgatcgg
      361 gatgacgctg gtcatcgtcg agttgctgac tggcacgttc tatctgctcg tgctcggaat
      421 cgcctgcttt ggcggaagcg cctctgcgtt tttcggccac acgttcggga cgcaggcaat
      481 cgttgcaggg tgtgttgccg cattgggagt ggtcctcgtg catgcctatc gggcgcgcaa
      541 tgccgccagg cagatggccc cgatagacgc cgggatgccg gcaaacttcg aatcttggat
      601 cgataagtcg gcgggtcttg cgcgcgttcg atatagaggc gcggcttggg acgcccgtat
      661 cgaagagagc gcttcactcg agcccggatc gctcgtttac gtggtggccg cgaatggcaa
      721 cacgctcaaa gtcaccgtca agcgccccgc ctgatttggc gggcgcgctc gctgccatac
      781 ggatagggag agtaccgatg ttgataaagc tggtcatcgc gcttgcgata tcgatcgcgg
      841 ccgcattcgt gcccgccacc gcgccgtacg ccgtgtggat cttcctgatt gccgtggtgg
      901 tagcattcgt catcgaaggc gtgcggatcg tgccgcaaca gcacgccttc gtcattgaac
      961 gactggggcg cttccacgcc atcctcgaac ccggtctcaa tctcatcatc ccgttcctcg
     1021 accgggtcgc ctacacacac aatttgaaag aagttccgct ggacgtgccg gaacagatct
     1081 gcatcacgag ggacaacacg cagctggggg tggacggtgt tctctactat caagtgaccg
     1141 acccgcgtct cgcgtcgtat ggctcgtcgg actacatcac ggccatcgtt cagcttgcgc
     1201 aaaccacgct gcgctcggag atcgggaaga tggagctcga taagaccttc gagtcgcgtg
     1261 aagagatcaa tcgccagatt gtcgcggcgc tcgatgaggc agggcgcaat tggggcatca
     1321 aggtccttcg ctacgaaatc aagagcctca ctccgccgga atcgatactg cgctccatgc
     1381 agcagcagat cacggcggag cgcgaaaagc gtgcgctgat cgcgaagtcg gagggcgagc
     1441 gccagcagga gatcaacatc gccgacggca agcgtcaggc ggcggtgctc gaatccgagg
     1501 gagaaaagca aggcgcgatc aatcgcgcgc aaggggaagc aaccgcgttg cgcctgatcg
     1561 cagatgcgaa cgcggcggcg gtgcgtgccg tcgcggaagc gattgcggac aagggcggca
     1621 tggatgcggc aaacctgaag gtggcgcagg aatacgtcgc agcattcgcc aacctggcca
     1681 agcagagcaa tacgcttatc atcccggcca acacgggtga cgtcgcggcg ttcgttggca
     1741 gcgcgatgac ggtgctcgac aagacgcgcc aggccgcagc gcagcggacg tgacggtttc
     1801 cgatttccgg ggcgggcgat catgaacccg tccgatcttc tgcagcgaat cgtcggcgcg
     1861 atgctcggca tcgcgctttt cgtcgccgcg ttcatcttcg cctcggtcct gctcgccatc
     1921 gccgcgaccg cggcgctgct gatctggggc tggctgtggt ggcgcacgcg cgagatccgc
     1981 cgtgccgcgc gcgaaggcga caacgtcgtt atcgagggtg aataccgcgt ggagcgcgag
     2041 agccgacggc tcgaggacga ctagctgccc gggcgcgcca gctggggcct cccggcggcg
     2101 cggcgccgct ccatgagttg cgcggtcacg acggcggcga gcagggcaag gccggccagg
     2161 tcgctgtaca gacctggctt gatcaggagc atcgccgcga tgaacagcgc cgtccgctgc
     2221 cacagcgcgg cgaaggcgag caaccagccg tgcaggctgg cggcgaggca gatcgctccc
     2281 atggtcgcgg tcactaccga cagcgcaacg tattcccact gcgtcgacca gtccttcacg
     2341 atcagcagca gggtcggctc gtagacgaac atgaacggca cgatgtaagc gggggcggcg
     2401 acacgcatcg ccgcaaagcc cgacttccac atgttggact tcgccaagcc cgcggccgcg
     2461 aacaccgcga gcgcgactgg cggggtaatt gcggaaagga tggcaaagta aaaggcgaac
     2521 atgtgcgcgg tcggggttgt cgcgccgagc ttgattaccg ccgggaccag cagcgccacc
     2581 atcatgatgt aagcgggggt ggtcggcatg cccatgccga gcacgatgcc ggcgagagcc
     2641 gtcaggatca gcgccagcac cagggagttc tgggcgagcg ccaccaccat ttgggtgaac
     2701 acgatgccga ggccggtgat ggtcacgcag gcgattacga cccccgcgca ggcgcaggcc
     2761 attgctaccg caagcgtgtt cttcgcgcca tcctcgagcg catcgatcac cgagcgccag
     2821 ccgatgcctt cacgcgtggc cttgcgcagc agcgccatcg gcaggcacgt gagcgcgccg
     2881 accagcgcgc aaagcggtgc cgagtaacct gccatcaggc cgaacaaaac catcaatatc
     2941 gggacgaaca ggtggccgcg gtcgaccatc accgagatga aacggggcag ctcggccttc
     3001 ggcacgccgg cgaggttgta gcgcttcgcc tcgaaatgca ccgcgaagaa cac
//
LOCUS       DASRVO010000659         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_382596, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000659 DASRVO010000000
VERSION     DASRVO010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382596"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VFF05_08145"
     CDS             complement(<1..1026)
                     /locus_tag="VFF05_08145"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010374868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZW51817.1"
                     /translation="MAKPIRILTGITTTGTPHLGNYVGAIRPAIAASRNAGADSFYFL
                     ADYHALVKCDDPARIRQSTLEIAASWLACGLDPEKVYFYRQSDIPEIPELTWLLTCVT
                     AKGLLNRAHAYKAANDANREKNEDADAGVTAGLYMYPVLMAADILIFNANKVPVGRDQ
                     VQHIEMARDMAQSFNHLYGEHFVAPEAAVDDSVAMLPGLDGRKMSKSYDNIIALFAPR
                     ERMRKQIAGIVTDSRAPGEPKQVEGSALFQIYQAFASAEETEALRRAYSEGIAWSDAK
                     QLLFERIDREIAPMRERHDALVANPAELERILREGADKARALAKPFLAELRSAVGLRD
                     LASKPVAA"
     gene            1145..>1924
                     /locus_tag="VFF05_08150"
     CDS             1145..>1924
                     /locus_tag="VFF05_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004137112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51818.1"
                     /translation="MKTLLSLAVVAALSVSAAARAGTLVDVTVVDRDSGATLPAYSHD
                     GKLYIAGTPGHRYGVRLVNRSGARVLAVLSVDGVNAISGETASPDQSGYVLDAYASTE
                     VNGWRKSLSEVAQFNFTNLGNSYAARTGRPDNVGVIGVAVFREKPLFWREGRIAQAPL
                     ADRYPPAPAAGMPAPRSADAASAKAESAPATAQATRPARAPQPEESLGTGHGAREHSD
                     VRYTQFVRASTNPDEVDAIWYDSQRNLVARGVIPAPRPIARE"
BASE COUNT          299 a          675 c          625 g          325 t
ORIGIN      
        1 cgccgcgacc ggcttggatg cgagatcgcg caggccgacg gccgaacgca attcggccag
       61 gaagggcttg gccagggcgc gcgccttgtc cgcgccttcg cgcaggatcc gctccagttc
      121 ggcggggttc gccaccagcg catcgtggcg ttcgcgcatc ggcgcgatct cgcggtcgat
      181 gcgctcgaac aacagttgct tggcatcgct ccacgcgatg ccctccgagt acgcgcggcg
      241 cagcgcctcg gtttcctcgg cggacgcgaa cgcctggtag atctggaaca gcgccgagcc
      301 ttccacctgc ttgggctcgc cgggcgcgcg cgagtcggtg acgatccccg cgatctgctt
      361 gcgcatccgc tcgcgcggcg cgaacagcgc gatgatgttg tcgtagctct tgctcatctt
      421 gcgcccgtcc aggcccggca gcatcgccac cgagtcgtcc acggccgcct ccggcgccac
      481 gaaatgctcg ccgtacaggt ggttgaagct ttgcgccatg tcgcgcgcca tctcgatgtg
      541 ctgcacctgg tcgcgcccga ccggcacctt gttcgcgttg aagatgagga tgtccgccgc
      601 catcagcacc ggatacatgt acagtcctgc ggtgacgccg gcgtcggcgt cctcgttttt
      661 ttcgcgattc gcgtcgttcg ccgccttgta ggcgtgcgcg cgattgagca gtcctttcgc
      721 ggtgacgcag gtcagcagcc aggtcagctc ggggatttcc gggatgtcgg actggcggta
      781 gaaatatact ttttccggat ccaggccaca ggccagccag ctcgccgcga tctccagcgt
      841 cgactggcgg atgcgcgccg gatcgtcgca cttgaccagg gcgtggtagt cggcgaggaa
      901 atagaacgaa tcggcgcccg cgttgcggct ggccgcgatc gccggacgga tcgcgccgac
      961 gtaattgccc aggtgcggcg tgccggtcgt ggtgatgccg gtgaggattc tgattggctt
     1021 ggccatgcga cacattccct agcgcaagtg cgcagtttac gcgatcgcca tgatgcgatt
     1081 aaccccgctc gcgctccagc cgcgttgcat gccccatcgt tcccccgcca agctggagat
     1141 gatcatgaaa accctgttgt cactcgccgt tgtcgccgcc ctgtccgtat ccgccgccgc
     1201 gcgggccggc accctggtcg acgttaccgt cgtcgaccgc gattccggcg ccaccctgcc
     1261 cgcctattcg cacgacggaa aactctacat cgccggcacg cccggccatc gctacggcgt
     1321 gcgcctggtc aaccgcagcg gcgcgcgcgt gctcgccgtg ctgtcggtgg acggcgtcaa
     1381 cgcgatcagc ggcgaaaccg cgagtcccga tcaatccggc tatgtgctcg acgcctacgc
     1441 cagcaccgag gtgaacggct ggcgcaagag cctgtcggaa gtcgcgcaat tcaacttcac
     1501 gaatctgggc aacagctacg ccgcgcgcac gggacgcccg gacaacgtcg gcgtgatcgg
     1561 cgtggccgtg ttccgcgaaa agccgctgtt ctggcgcgag ggtcgcattg cgcaagcacc
     1621 gctcgccgac cgctacccgc ccgcgccggc ggctggaatg cctgcgccac gttccgcgga
     1681 cgcggccagc gccaaggccg aatcggcgcc tgcgacagcg caggcgacgc gtccggcccg
     1741 cgcaccgcag cccgaagaat cgctgggcac cggccacggg gcgcgcgaac attccgatgt
     1801 gcgctacacg caattcgtgc gcgccagcac gaacccggac gaagtcgatg cgatctggta
     1861 cgacagtcaa cgcaacctcg tcgcgcgcgg cgtgatcccg gcaccgcgcc cgatcgcacg
     1921 cgag
//
LOCUS       DASRVO010000660         5817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_123526, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000660 DASRVO010000000
VERSION     DASRVO010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123526"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VFF05_08155"
     CDS             complement(<1..371)
                     /locus_tag="VFF05_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZW51819.1"
                     /translation="MDAIYDFKAVEAAAQKFWNDGKSFEAKEDPARPKFYCLSMLPYP
                     SGALHMGHVRNYTIGDVISRYQRMLGKNVLQPMGWDAFGLPAENAAIKNNTAPAKWTY
                     ANIDHMRSQLKSLGYAIDWTR"
     gene            483..1676
                     /locus_tag="VFF05_08160"
     CDS             483..1676
                     /locus_tag="VFF05_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HZW51820.1"
                     /translation="MPDTSQSGSLASLSRLCGELAGERPLPERLNAILDAACAMAHAD
                     TGTIGLYDPQTDTIAAAVTRHSMPTQFATHFVRGQGLAGHIIATMQPYLGRYGDLPQP
                     VVESLRDHQSLGVPLLWQGRLLGYLALSLEPPRRFKSADPEMAQVLAHAAAAAIENAT
                     LHEQAGRAAVLAERQRLARELHDNVTQILASINLLSQTIAATWKRNPAEGEARAARLQ
                     QLAQTAFAEMRMLLQQLTPAESAATAPAISRKSFVLLSAENLRTLGLPGALTRLLAST
                     VPEHIATASSFATWEAQREEHEQALYRVCQEAISNCLRHAGARRLRVEAAVTAEHAVL
                     RVSDDGRGLGTDFRPGMGLGSMRSRIEALHGSFRIGSNSPAGTLIEARLPRADRDPSA
                     PEAQP"
     gene            1729..2409
                     /locus_tag="VFF05_08165"
     CDS             1729..2409
                     /locus_tag="VFF05_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZW51821.1"
                     /translation="MKNAAASANSPATDATPIRIILIDDHAVVREGVRAVLETDADFS
                     VVGEFGSGNEALASVAALAPDVALVDLKMPGLSAPETIAALRERIPGVRVLVFTSFGE
                     DALIRATLDAGATGYLLKDALQHDLISAIRSVAAGETYLAPAAQRQLMELLRKPQASR
                     DALTARETDVLKLIAEGMSNKQIGKRLSLTEGTVKGYVSQILAKLRLEDRTQIALYAV
                     RHGLAAGA"
     gene            2489..3814
                     /locus_tag="VFF05_08170"
     CDS             2489..3814
                     /locus_tag="VFF05_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZW51822.1"
                     /translation="MRLVPLAAAVAVLFAVPTFAQSPAAPVTVVQCGHLIDVAHGKML
                     GASTIVIEADRVKEVLDGTQQRAGANTLSMPNATCMPGLIDSHTHLTGQFSKSSYSDQ
                     FRLNPADYAIQGTVFARRTLLAGFTSVRNVGDNNNESIALRNAVNKGEVPGPRILTAG
                     VPIGSTGGHADGSDGYRLDLQGDPTPKDGIINSPEDAWKAVRQHYKDGADLIKIMPSG
                     GVLDESSSSQNPQLTLAEIQAVVAAAHDYGFTVAAHAHGAEAIRRAVIGGVDSIEHGT
                     FMNDEDMKLMKEHGTWYVPTIIAGQFVMGKAKEGWYPPQVARKAMEVGPIIMQTAGKA
                     YKAGVRIAFGTDAGVYPHGENAKEFEYMVEAGMPPMFTIQAATTHAAQLLKHDKDVGS
                     LEPGKFADIVAVPGNPLNDIKLMQKVDFVMKGGVVYEQDGKPIAASLDK"
     gene            3898..5397
                     /locus_tag="VFF05_08175"
     CDS             3898..5397
                     /locus_tag="VFF05_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-60 repeat domain-containing protein"
                     /protein_id="HZW51823.1"
                     /translation="MPNSISRVSLVAAMAVCVSAWAGPATDFGALDSTFGSGGEARLT
                     VAPFNQPAGAALVANDIVVQPDGKILLAGYAAASTSEWIVIRLNANGTYDSTFGTGQN
                     GIAYIYTFGPADNQAASIALRPNGKILIGGTINDGNNGLVTAVVVQLNSDGSFDTTWG
                     NNGQVYFTPAAGDATRARAIVLDTVDPYAFGTLYLVGQYTHGGSPTCNNFFYAGISPD
                     GKTKVSNAYQASESCNVSESATSVAVQPGTGNVIVGGYSAFVSGSTRCSAISTVIVPS
                     SVFPTAYLYTPWGSNGATSFTVNGAQFPNDFCDGMTITPGGSVFLAGHGTAATGSGTY
                     QAAIIGWLDANGAVVSYGLGAQALTSFDYTETSLIVNDANTVNKLIVQPYGSLLLPAG
                     YNNDSHNAPYAGDDFAFERFSVSSQNGVLRDSAFNNGAGVMLDWGSYHTPVSSYISNE
                     RVQSAAFDPQGRLIVAGYTSDSAGGTDIAVARFAPFDGIFKNGFETPSY"
     gene            5430..>5817
                     /locus_tag="VFF05_08180"
     CDS             5430..>5817
                     /locus_tag="VFF05_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HZW51824.1"
                     /translation="MKRIPLSFLASAICAAVALGAGPTMAQDKPASPMDQLDSFVGDG
                     TCTGNVMAMNKHPGHATTGKYHGEKTLDGHWVVIQYDEDQSAANPKPFRVVQYFGYDA
                     AKKRFVSVLIDNSDPGYATSMSPGWKD"
BASE COUNT         1132 a         1945 c         1740 g         1000 t
ORIGIN      
        1 tcgcgggtcc agtcgatcgc atagcccagc gatttcagct ggctgcgcat gtggtcgatg
       61 ttcgcgtagg tccatttcgc cggcgcggtg ttgttcttga tcgcggcgtt ttccgccggc
      121 agaccgaacg catcccagcc catcggctgc aatacatttt ttccgagcat gcgctgatag
      181 cggctgatca cgtcgccgat ggtgtagttg cgcacatggc ccatgtgcag cgcgccggac
      241 ggataaggaa gcatcgacag gcaatagaac ttcgggcgcg ccgggtcctc ctttgcctcg
      301 aaggatttgc cgtcgttcca gaacttctgc gcggcggctt ccaccgcctt gaaatcgtag
      361 attgcgtcca tcggagccct aaatgcgccg gtcgcaccgg cttttcttcg aatgcgtgca
      421 gcctaccgca gcgcagcatt cccgccaacc ccggcgacgc ccgcttcggt taggctagag
      481 gcatgcccga cacatcacag tccggttcgc tcgcctcgct ctcgcgactt tgcggagagc
      541 ttgccggcga acgccccttg ccggaacggc tgaacgcgat tctggacgcg gcctgcgcga
      601 tggcccacgc ggacaccgga acgataggct tgtacgaccc gcaaaccgat accatcgcgg
      661 cggctgtcac ccggcattcg atgccgaccc agttcgccac gcatttcgtt cgtggccaag
      721 gcttggcagg tcacatcatt gcaacgatgc agccgtacct gggccgttat ggagatttgc
      781 cgcagccggt ggtcgagagt ttgcgcgatc atcaatcgct gggcgtgccg ctgctctggc
      841 aagggcgctt gctcggctat ctggccctga gccttgaacc gccgcgccgg ttcaagtccg
      901 ccgacccgga aatggcgcag gtgctggccc acgccgccgc cgcggccatc gaaaacgcga
      961 ccttgcacga acaggccgga cgcgccgccg tgctggccga acgccagcgt ctggcgcgcg
     1021 agctgcacga caacgtcacc cagatcctcg cctcgatcaa tctgctcagc cagaccatcg
     1081 ccgccacgtg gaagcgcaat cccgcggagg gcgaggcgcg cgccgcgcgc ctgcagcagt
     1141 tggcgcagac cgctttcgcc gagatgcgga tgctgctgca acagctcacg cccgccgagt
     1201 cggcggcaac ggcgccggca atctcgcgca agagcttcgt gctgctcagc gccgaaaacc
     1261 tgcgcacgct gggactgccc ggcgcattga cccggctgct cgcatcgacc gtccccgagc
     1321 acatcgccac ggcgtcgagc ttcgcgacct gggaagcgca gcgagaggaa cacgaacagg
     1381 cgctgtaccg ggtgtgccag gaagccatct cgaattgcct gcgccacgcc ggtgcacgcc
     1441 gcctgcgcgt ggaagccgca gtcacagccg agcatgccgt gctgcgcgtt tccgacgatg
     1501 gccgtggcct gggcacggat tttcgccccg gcatgggcct gggcagcatg cgcagccgga
     1561 tcgaggcgct gcacggcagc ttccgcatcg gctcgaacag tccggccgga accctgatcg
     1621 aggcgcgcct gccgcgtgcg gaccgcgatc catccgcgcc ggaggcccaa ccctgacttt
     1681 ggtcagtgcc gccgcccctg accatgcggc acactgcctc tcatcgtcat gaaaaatgcc
     1741 gccgccagcg caaattcccc tgccacggac gccacgccga tccgcatcat cctgatcgac
     1801 gatcacgctg tggtgcgcga aggcgtgcgc gcggtgctgg aaacggatgc ggatttcagt
     1861 gtcgtcggcg agttcggcag tggcaacgag gcgctggcct cggtcgccgc gctggcgccg
     1921 gatgttgcct tggtcgatct gaaaatgccc gggctgtcgg caccggaaac gatcgcggcc
     1981 ttgcgcgagc gtatacccgg cgtgcgcgta ctggtgttca cgagtttcgg cgaggacgcg
     2041 ttgatccgcg ccacgctcga tgccggcgcc accggctacc tgctcaagga tgcgttgcag
     2101 cacgacctca tctccgccat ccgcagcgtg gccgccggcg aaacctatct ggcgcccgcc
     2161 gcgcaacggc aattgatgga actgctgcgc aagccgcaag catcccgcga cgcgctcacc
     2221 gcacgcgaaa ccgacgtgct taaactcatt gccgaaggaa tgtcgaacaa gcagatcggc
     2281 aagcgcttga gtctcaccga aggcacggtc aagggctacg tcagccagat ccttgccaag
     2341 ctgcgcctcg aggatcgcac ccagatcgcg ctctacgccg tgcgccacgg actcgccgcc
     2401 ggcgcttgaa cgcaccgcgc aggacttttg gcctacgcgc cgcccggtac acagcgtatc
     2461 cttccacgat caccaaggga ggataaccat gcgtctcgtt ccgctcgccg ccgctgtcgc
     2521 ggtcttgttc gccgtgccca cgtttgcaca atcgcctgct gcaccggtca ccgtcgtgca
     2581 gtgcggacat ctgatcgatg tggcgcatgg caagatgctc ggcgccagca ccatcgtcat
     2641 cgaagccgac cgcgtaaaag aggtcctcga cggcacccag cagcgcgccg gcgcgaacac
     2701 gctatccatg ccgaacgcca cctgcatgcc cggcctgatc gacagccaca cgcaccttac
     2761 cggccagttc agcaagtcca gctacagcga ccagttccgc ctgaatccgg ccgactacgc
     2821 gatccagggc acggtcttcg cgcgccgcac cctgctcgcc ggattcacca gcgtgcgcaa
     2881 tgtcggcgac aacaacaacg aatccattgc cctgcgcaac gccgtgaaca agggtgaagt
     2941 gcctggaccg cgcatcctca ccgccggcgt gccgatcggt tccaccggcg ggcatgccga
     3001 cggaagcgac ggctatcgcc tggatctgca gggcgacccg acgcccaagg acggcatcat
     3061 caactcgccc gaggatgcct ggaaggcggt gcgccagcac tacaaggacg gcgccgacct
     3121 gatcaagatc atgccttccg gcggcgtgct cgatgaaagt tccagttcgc agaatccgca
     3181 gttgacgctc gcggaaatcc aggccgtggt cgccgccgcg cacgattacg gcttcactgt
     3241 ggccgcgcat gcgcacggcg ccgaggcgat ccgccgcgcc gtgatcggcg gcgtggactc
     3301 catcgagcac ggcaccttca tgaatgatga ggacatgaag ctgatgaagg agcacggcac
     3361 ctggtacgtg ccgacgatca ttgctggcca gttcgtgatg ggcaaggcaa aggaaggctg
     3421 gtatccgccg caggtcgcgc gcaaggcgat ggaagtcggt ccgatcatca tgcaaaccgc
     3481 cggcaaggcc tacaaggccg gcgtcaggat cgcgttcggc accgacgccg gcgtgtatcc
     3541 gcacggcgag aatgccaagg aattcgaata catggtcgag gccggcatgc cgccgatgtt
     3601 cacgatccag gccgcgacca cgcacgccgc gcaactgctc aagcatgaca aagacgtggg
     3661 cagcctggaa cccggcaagt tcgccgacat cgtcgccgtg ccgggcaatc cgctcaacga
     3721 catcaagctg atgcagaaag tcgacttcgt gatgaagggc ggcgtggtgt acgagcagga
     3781 tggcaagccg atcgccgcgt cactcgacaa ataaacgcat ggccgggtgt cagtaaccgg
     3841 cctcgcatga cgtaacccgt tccgaacccg ccacaatggc ggggatcgga acgggccatg
     3901 cccaattcaa tttcacgcgt ttccctcgtc gctgcgatgg cggtttgcgt gtctgcttgg
     3961 gccgggccgg caacggattt cggcgcattg gactccacat tcgggtccgg cggcgaagca
     4021 cggctgacgg tcgcgccctt caaccagcct gccggcgccg ccctggtcgc caacgatatt
     4081 gttgtccaac ccgacggcaa gatcctgctg gctggctacg cggcggcaag caccagcgaa
     4141 tggatcgtca ttcgcctcaa tgccaacggt acatacgact ccacgttcgg cactggccag
     4201 aacggcatcg cctatatcta cacgtttggt ccggcggaca accaagccgc cagcatcgcg
     4261 ttgcgaccga acggcaagat cctcattggt ggcacgatca acgatggcaa caacggactg
     4321 gtcacggctg ttgtggttca gctcaatagc gacggcagct tcgacaccac ctggggcaac
     4381 aacggtcagg tctacttcac gccggccgct ggcgacgcga cgagagcgcg cgctatcgtg
     4441 ctcgacacgg tggatccata cgcattcggc accctctacc tcgtgggaca gtacacgcac
     4501 ggcggctcgc caacctgcaa caacttcttt tatgcaggca tctcaccgga tgggaagaca
     4561 aaagtttcca acgcctatca ggccagcgag agttgcaacg taagcgaatc ggcgaccagc
     4621 gtggccgtgc aacccggcac cggcaatgtg attgtcggcg gctactctgc atttgtctcc
     4681 ggctcgaccc gctgcagcgc aatcagcacg gtgatcgtcc catcgtccgt ttttccaacg
     4741 gcatatctat acacaccatg gggcagcaac ggcgcgacct cctttaccgt aaatggcgcg
     4801 cagtttccca acgatttctg cgatggcatg acgatcacgc ctggaggatc cgtctttctc
     4861 gccggccacg gcactgcggc aacgggaagc gggacctatc aggcggcaat catcggctgg
     4921 ctggacgcca atggggccgt cgtcagctac ggccttggtg cgcaagcgct gacctccttc
     4981 gattacaccg agacgagcct gatcgtcaac gacgcaaaca ccgtcaacaa gctgattgtg
     5041 cagccctacg gatctctatt gctgccggcc ggctacaaca acgactcgca caatgctcca
     5101 tatgcaggcg acgacttcgc atttgaacgc ttcagcgtgt cgagccagaa cggtgtgctt
     5161 cgggattctg ctttcaacaa cggagccggc gtgatgctgg attggggttc gtaccacaca
     5221 cctgtatcca gctacatcag caacgagcgc gtgcaatcgg cggctttcga tccgcaaggc
     5281 cgattgattg tagccggata taccagcgat tcggctggcg gcaccgacat cgctgtcgcg
     5341 cgcttcgcgc ccttcgacgg catcttcaag aacggtttcg agacgccgtc gtattgagtc
     5401 aatgttgtta acccaacggg agaacactca tgaaacgcat ccccttatca ttcttggctt
     5461 ccgccatctg cgcagcagtc gcccttggcg ccggaccaac aatggcgcag gacaagccag
     5521 cctcgccgat ggaccaactc gacagcttcg tcggcgacgg cacctgcacc ggcaatgtga
     5581 tggcgatgaa caaacaccct ggccatgcca ccaccggcaa gtaccatggc gagaaaaccc
     5641 tggacggcca ctgggtggtg atccaatacg atgaagatca aagcgcggcc aatccaaagc
     5701 cgttccgtgt cgtgcagtac ttcggctacg acgcggcaaa aaagcgcttc gtctccgtac
     5761 tcatcgacaa ttccgacccg ggctacgcca cgagcatgag ccctggctgg aaagacg
//
LOCUS       DASRVO010000661         1213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_279833, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000661 DASRVO010000000
VERSION     DASRVO010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279833"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFF05_08185"
     CDS             complement(<1..495)
                     /locus_tag="VFF05_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51825.1"
                     /translation="MKLVVKPLSLACALALGLAFSATVPTSAFAEPAHATASADQATT
                     QTAPKLHAAMRGLWHGHLVATHDYALAVHAGNKAHENSAADAVVANAKDLANAVAGFY
                     GKDAGDGLFKLLAGHWGGVKAMTDNAKSGDAAGEKKALEASLTNAADIATFLAGANPN
                     WTEGA"
     gene            complement(611..>1213)
                     /locus_tag="VFF05_08190"
     CDS             complement(611..>1213)
                     /locus_tag="VFF05_08190"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZW51826.1"
                     /translation="TDLPVNADFESGYARDIDALKNNVRRCVETGVAGLSIEDSSGDD
                     SQPLFELDEAIARLRAARAAIDATGEDVVLVGRAECYLVGHADALNESIKRLRAYAQA
                     GADCLYAPGVRDPEHIRAIVAAVAPKPVNVLVGYPAPLSLNDFVQIGVRRISVGGALA
                     GAAWGSFLRAARSLAQGRFDGFADNASHAELGALFARNVD"
BASE COUNT          226 a          392 c          371 g          224 t
ORIGIN      
        1 cgcgccctcg gtccagttcg gattcgcgcc ggcgaggaat gttgcgatat ccgccgcatt
       61 ggtcagcgat gcttccagcg ccttcttctc gccggcggcg tcgccggact tggcgttgtc
      121 ggtcatggcc ttgaccccac cccaatggcc ggccagcaat ttgaacaagc cgtcgcccgc
      181 gtccttgccg taaaacccgg ccaccgcatt ggccaggtcc ttggcattgg cgaccaccgc
      241 atcggctgcg gaattctcat gagccttgtt gcccgcgtgc acggccagcg cgtaatcatg
      301 ggtagcgacc agatgtccgt gccacagtcc gcgcatcgca gcatgcaatt tcggcgcggt
      361 ttgcgtggtc gcctgatctg cactggcggt cgcatgcgcc ggctcggcga aagccgaagt
      421 tggcacggtg gcggaaaatg ccaggcccag cgcaagcgca caggccagtg aaagcggttt
      481 cacgacgagt ttcatgatca atctccattg gacgatcgcc ggttggcgat gcgtccatta
      541 tgggagcttg aaacataaat caaatgtaga tatgtgtaaa gctttttgcg gaagcgttta
      601 tgtacggtta tcaatcaaca tttcgggcaa acagtgcgcc gagttcagcg tggctcgcat
      661 tgtctgcgaa accgtcgaat cgcccttgcg caaggctgcg cgcggcacgc aggaaactgc
      721 cccaggcggc gccggccaag gcaccgccga cgctgatgcg acgcacgccg atctggacga
      781 aatcgttaag cgaaagcggc gcgggataac cgaccagcac gttcaccggc ttcggcgcga
      841 ccgccgcgac gatcgcgcga atgtgttccg gatcgcgcac gcccggcgcg tacaggcaat
      901 cggcaccggc ctgcgcatag gcgcgcaggc gcttgatcga ctcgttcagc gcatcggcat
      961 gtccgacgag atagcactcg gcgcgaccga ccagcaccac gtcctcgccc gtcgcgtcga
     1021 tcgccgcacg cgcggcacgc aaccgcgcga tcgcttcgtc gagttcgaac agcggttgcg
     1081 aatcgtcgcc gctggagtcc tcgatcgaca agcctgcgac gccggtctcg acgcagcggc
     1141 ggacattgtt cttcaacgca tcgatgtcgc gcgcgtagcc gctttcgaaa tccgcattga
     1201 ccggcagatc cgt
//
LOCUS       DASRVO010000662         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_227590, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000662 DASRVO010000000
VERSION     DASRVO010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227590"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..>1049)
                     /gene="hisG"
                     /locus_tag="VFF05_08195"
     CDS             complement(239..>1049)
                     /gene="hisG"
                     /locus_tag="VFF05_08195"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF013773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZW51827.1"
                     /translation="SEIASQLARGMVHLGVTGEDLVRESIADADRRVLLIDSLGFGSA
                     NVVVAVPQAWIDVRTMADLDDVTTGFRARHNRRMRVATKYINLTRTFFASHGVVDYRI
                     VESAGATEGAPAVGTAEMIVDITTTGATLAANGLKVLDDGVILRSQANLVASKEADWS
                     SEARETARVILDHIAARARASKYREVRTRFGGCDAALLAEAHNRFGVVSPFGGPTSSG
                     MVTLHCPPAQLYALGSFLRAHGADTVSVASLDYVLDRDNPLFARLEAFLRH"
BASE COUNT          190 a          347 c          343 g          169 t
ORIGIN      
        1 cgtagcgctg ccgcagcgtc ttggccagag cgttcaggcg tccatgcaga agcatcagcc
       61 cggccgcttc attataaacg ggcacgacga tcgacagccc ctgcgccgcg gcgctggccg
      121 cggcggtgga caggctagaa acatccgtac ccagcatcat agatccggtt ccaatcacca
      181 ctccatatgg caattgccgc gcattttcgc cacgggaaag gacatgcggc gctacgcctc
      241 agtgccgcag gaatgcctcg agcctggcaa ataacggatt gtcgcgatcg aggacatagt
      301 cgagcgacgc caccgacacc gtatcggcgc cgtgcgcgcg aaggaagctg ccgagcgcgt
      361 aaagctgcgc cggtgggcag tgcagcgtaa ccatgccgga agaggttggg ccgccaaacg
      421 gcgacaccac gccgaaccgg ttatgggctt cggccaaaag cgcggcgtcg cagccgccaa
      481 agcgggtgcg gacctcacga tatttgctgg cgcgggcgcg agcggcgatg tgatctagga
      541 tgacgcgcgc ggtctctctc gcctcgctcg accaatcggc ctccttcgac gccaccaggt
      601 tggcctggct gcgaaggatc acgccgtcgt cgagcacctt gagaccgttg gcggcgagcg
      661 tcgcgccggt cgtggtgatg tcgacgatca tttccgccgt gcccaccgcc ggcgcacctt
      721 cggtcgcacc ggcgctctcg acgatgcggt agtcgacgac gccgtgggag gcgaagaagg
      781 tccgggtcaa attgatgtat ttggtcgcca cccgcatccg ccggttatgc cgggcgcgga
      841 agccggtggt gacgtcgtcg agatcggcca tggtgcggac gtcgatccag gcctgcggca
      901 ccgccaccac gacgttggcg ctgccaaagc cgaggctgtc gatcagcagc acgcgcctgt
      961 ccgcatccgc aatgctttcg cgcaccagat cttcgccggt gacgcccaga tgcaccatgc
     1021 cgcgcgcgag ctgcgaggcg atctcgctc
//
LOCUS       DASRVO010000663         1368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_201652, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000663 DASRVO010000000
VERSION     DASRVO010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201652"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1368)
                     /gene="pepN"
                     /locus_tag="VFF05_08200"
     CDS             complement(<1..>1368)
                     /gene="pepN"
                     /locus_tag="VFF05_08200"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HZW51828.1"
                     /translation="GDYRIEADGALVIDTVPEQFALDIEARIAPEHNTALSGLYVSGG
                     NFCTQCEPEGFRRITYFVDRPDVMARYSVTIRADKARYPVLLSNGNPAGRGDLPDGRH
                     WARWVDPHPKPSYLFALVAGDLVAVEDSFLTRSGRAVQLAIWVRRGDEDKCGHAMQSL
                     KKAMRWDEDRFGLEYDLDVFNIVAVSDFNMGAMENKGLNIFNTRYVLAKPETATDSDY
                     QGIETVIAHEYFHNWTGDRVTCRDWFQLSLKEGLTVFRDQLFSADQGSAAVCRIGNIR
                     TLRAAQFPEDAGPLAHPVQPQSYLRIDNFYTATVYNKGAELIRMMHTLLGADGFRRGM
                     DLYIAENDNHAATIGDFLAAMRQAGAERGVDLGQFERWYEQAGTPEVTVEDDYDPTAR
                     RYSLSVAQRIPPTPGQPDKMAVPLPLAIGLIGPNGDELPTRLDGVAATQAGGRVLVAA
                     EARQD"
BASE COUNT          224 a          447 c          444 g          253 t
ORIGIN      
        1 aaatcctgtc gcgcctcggc ggcgacgagc actcgcccgc ccgcctgggt cgctgcgacg
       61 ccgtccagcc gggtcggcag ttcgtcgccg ttgggtccga tcagcccgat tgccagcggc
      121 agcggcaccg ccatcttgtc gggctggccg ggggttggcg ggatgcgctg cgcgacgctc
      181 agcgaatagc ggcgcgccgt cgggtcgtaa tcgtcctcga ccgtgacctc tggcgtgccg
      241 gcttgttcgt accagcgctc aaattggccg agatcaacgc cgcgctcggc tcccgcctgt
      301 cgcatggccg cgaggaagtc gccgatcgtc gcggcatggt tgtcgttttc cgcgatgtag
      361 agatccatgc cgcggcggaa gccgtcggcg cccaagagcg tgtgcatcat gcgaatcagt
      421 tcggcgccct tgttgtagac ggtcgcggtg taaaaattgt cgatgcgcag atagctttgc
      481 ggctgcaccg gatgcgccag cggcccggca tcctcgggga actgcgcggc gcgcaatgtg
      541 cggatattac cgatgcggca caccgcggcg ctgccctggt cggccgagaa cagctggtcg
      601 cgaaataccg tcagcccttc cttcagcgac aactggaacc agtcgcggca ggtgacccgg
      661 tcgccggtcc agttgtggaa atactcgtgc gcgatgactg tctcgatgcc ctgataatca
      721 ctatcggtcg cggtctcggg cttcgccaat acgtagcgcg tgttgaagat gttgaggccc
      781 ttgttctcca tcgcccccat gttgaaatcg ctgacagcaa cgatgttgaa gacgtcgagg
      841 tcgtattcga ggccgaacct gtcctcgtcc cagcgcatcg cctttttcag cgattgcatc
      901 gcgtggccgc atttgtcttc gtcgccgcgg cgcacccaga tcgccagctg caccgcgcgg
      961 ccggagcgcg tcaggaagct gtcctcgacc gcgacgagat cgccggcgac cagcgcaaac
     1021 agatacgacg gcttcggatg cggatcgacc cagcgcgccc aatgccgccc gtccggcaga
     1081 tcgccgcggc ccgccggatt gccgttcgac agcagcaccg gatagcgcgc cttgtcggcc
     1141 cggatcgtca cgctgtaacg cgccatcaca tcggggcgat cgacgaaata ggtgatgcgg
     1201 cgaaacccct ccggttcgca ttgcgtgcag aaattgccgc cggagacata aagaccggac
     1261 agcgcggtgt tgtgctccgg tgcgatacgg gcttcgatgt cgagcgcgaa ctgctccggc
     1321 acggtgtcga tgaccagcgc gccgtccgct tcgatgcggt agtcgccc
//
LOCUS       DASRVO010000664         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_46241, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000664 DASRVO010000000
VERSION     DASRVO010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46241"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFF05_08205"
     CDS             <1..389
                     /locus_tag="VFF05_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZW51829.1"
                     /translation="SCSGGRHAFIYACQRKDIDACVEQWGGNVVMAKADLNEKKPVAP
                     IDMTKDLSCPLIGLFGNEDRAPSPEQVNQHEAELKKHGKAYEFHRYDGAGHGFFYWNR
                     PAMYRIEQAMDGWTKVYAFLGKHLAK"
     gene            417..722
                     /locus_tag="VFF05_08210"
     CDS             417..722
                     /locus_tag="VFF05_08210"
                     /inference="COORDINATES: protein motif:HMM:NF039404.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6295 family protein"
                     /protein_id="HZW51830.1"
                     /translation="MCTSIIEIVRAEGMGKRGDEWFPLTQAVVAYDHARHAPLGDVIT
                     LDFINSALDPGARAGVELTLESAAALRDALDRAIAAAEFEEAEIRGKGVVLSFARTG"
     gene            complement(846..>1905)
                     /locus_tag="VFF05_08215"
     CDS             complement(846..>1905)
                     /locus_tag="VFF05_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW51831.1"
                     /translation="RLRNLEKMRDERSRRIVHKTAKLGNVASDAGGRLVAELEHVDKA
                     FGDKVIARDFSTRVLRGDRVGVIGRNGAGKSTLLGILAGTIEPDNGVARHGTNLTLAI
                     FDQLRESLDPDSTPWATLTDGGGDTLAVQGRTRHVASYLKDFLFEERQFKAKINSLSG
                     GERNRLLLAKVLAKTSNLLILDEPTNDLDMETLDLLEEVLADYEGTLLLVSHDRDFLD
                     RLVTSIIAVEGEGVVHEYVGGYTDYLRQRREPLAPRATRAVEARPAPEPQRNRAASKL
                     SFKDQRELDGLPERIAKLAAEKVEVENLLADPAGAADAAKLKAGMVRHGELVAELAAA
                     EERWLALAERAEELASNR"
BASE COUNT          351 a          600 c          620 g          334 t
ORIGIN      
        1 ggtcgtgctc gggcggccgg cacgctttca tctacgcctg ccagcgcaag gacatcgatg
       61 cctgcgtcga gcaatggggc ggcaacgtgg tgatggccaa ggcggacctc aacgagaaaa
      121 agccggtcgc gccgatcgac atgacgaagg acctctcatg tccgctcatc ggcctgttcg
      181 gcaatgagga tcgcgcgccg agccccgagc aggtgaacca gcacgaggcc gagctcaaga
      241 agcacggcaa ggcgtatgaa ttccatcgct acgacggcgc cggccacggc ttcttctatt
      301 ggaaccggcc ggcgatgtat cgcatcgaac aagcgatgga tggctggacc aaggtctacg
      361 ccttccttgg aaagcacctg gcgaagtaag agcccggcga taagaggaga tagggcatgt
      421 gcacatcgat catcgagatc gtccgcgccg aaggcatggg caaacgcggc gacgaatggt
      481 tcccgttgac gcaggccgtg gtcgcctacg accacgcgcg ccatgcgccg ctcggcgacg
      541 tcatcacgct cgatttcatc aatagcgcgc tcgatccagg ggcgcgggcg ggtgtcgagc
      601 tgacgctcga atcggccgcg gcgctgcgcg atgctctcga ccgcgcgatt gccgccgccg
      661 agttcgagga agcggaaatt cgtggcaagg gtgtcgtgct cagcttcgcg cgaacggggt
      721 gacggccgag cgcacgagta tagtctgcgc gatcgctgac gcgcgccgtg ccggtgcgcg
      781 cgaccgccgt cggggcggcg ctcaccggca acacgcccga tgacaagatc ttccgcgacg
      841 cggcttcagc gattggacgc cagttcctcg gcgcgttcgg cgagggccag ccagcgttct
      901 tcggctgccg caagctcggc caccaattcg ccgtgacgga ccatgccggc cttgagcttg
      961 gcggcatcgg cggcgccggc aggatcggca agcagatttt cgacctcgac cttctccgct
     1021 gcgagcttcg cgatgcgctc tggcaagcca tcgagctcgc gctgatcctt gaaggacagc
     1081 ttcgacgcgg cacgattgcg ttgcggctcc ggcgccggtc ttgcttcgac agctcttgtc
     1141 gctctcggcg cgagcggctc gcgacgctga cgtagatagt cggtatagcc gccgacatat
     1201 tcatggacga cgccttcccc ttccacggcg atgatgctgg tgacgaggcg atcgaggaaa
     1261 tcgcggtcgt gactgaccag cagcaaggtg ccctcgtaat cggccagcac ttcttcgagg
     1321 agatcgagcg tctccatatc gagatcgttg gtcggctcgt cgagaatcag cagattcgat
     1381 gtcttcgcca gcaccttcgc cagcaagagc cgattgcgct cgccgcccga cagcgaattg
     1441 atcttggcct tgaactggcg ctcctcgaag aggaagtcct tgagatacga cgcgacatga
     1501 cgcgtgcggc cctgcacagc gagtgtatcc ccgccgccat cggtcaaggt ggcccagggc
     1561 gtcgaatccg gatccagcga ttcgcgcaac tgatcgaaga tcgcgagggt gagattggtt
     1621 ccgtgacgcg caacgccgtt gtcgggctcg atcgttccgg ccagaatgcc gagcagggtc
     1681 gatttgcccg cgccgttgcg gccgataacg ccgacgcgat cgccgcgcag cacgcgggtc
     1741 gaaaaatcac gcgcgataac cttgtcgccg aaagccttgt cgacatgctc gagttcggcg
     1801 acgagacggc cgccggcatc cgaggcgaca ttgccgagct tcgccgtttt gtgcacgatt
     1861 cgtcgcgaac gctcgtcgcg catcttctcg agattgcgca agcgg
//
LOCUS       DASRVO010000665         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_148439, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000665 DASRVO010000000
VERSION     DASRVO010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148439"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1054
                     /locus_tag="VFF05_08220"
     CDS             <1..>1054
                     /locus_tag="VFF05_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZW51832.1"
                     /translation="LADAQKRLETGNREIIAARRALDVAASGVIIAGARPNPTISTEW
                     TSTVPSSGLGAVRNKQYDSVLHVDQLVERGGKRELRIATADALVQAARRDVAEVRRQQ
                     GLALEIAYYDLKLAQEKLRIQNDTALLQQQSVNATQRRFEAGDASESEVVRLRVEALR
                     AANDARAAEADLIRARQDVAILIGEEGKAGALTAVDDWPAGLAADATAEPDPASIEAR
                     PDVRAAQARVDSAEQARELARRLLTRDVMVGAQVERLFPPDPGIGFGGTGYGVSFSVP
                     LFLRYGFEGEVAQAEAQLTSAMAARDRQVVIALAEVRRARNDVVSAAERRRRLTEELL
                     PQAKRAMDAAEFAYQRG"
BASE COUNT          183 a          341 c          358 g          172 t
ORIGIN      
        1 cgctggccga cgcccaaaaa agactggaaa cgggcaaccg cgagatcatc gcagcgcgcc
       61 gtgcgctcga cgtcgccgct tcaggggtca taatcgcggg cgcccggccc aatcccacca
      121 tcagcacgga atggacgagt accgttccct catccggttt gggtgcagtt cggaacaagc
      181 agtacgacag tgtcctgcac gtcgatcaac tcgtcgagcg cggcggaaag cgtgaacttc
      241 gcattgccac ggctgatgcg ctggttcagg ccgcgcgccg ggacgttgcc gaggttcgcc
      301 gccagcaggg acttgcgctg gaaatcgctt actacgactt gaagctcgcc caggaaaaac
      361 tgcgcatcca gaacgacacg gcgcttttgc agcagcaatc ggtcaacgcc acacagcgcc
      421 gcttcgaggc gggcgatgct tctgaatccg aagtggttcg attgcgggtc gaggcgctgc
      481 gcgctgccaa tgacgcgcgc gcggccgagg ccgatctcat tcgggcacgt caggacgttg
      541 ccatcctgat cggcgaggaa ggcaaggctg gcgccctgac agcggtggat gattggcctg
      601 ccggccttgc cgctgacgcg accgcggagc ccgatcctgc ttccattgaa gcgcggccgg
      661 acgtgcgcgc agctcaggct cgcgtcgatt ccgccgagca ggctcgagag ctcgctcgcc
      721 ggctgctcac ccgcgacgtg atggtggggg ctcaggtgga gcgattattc ccgccggatc
      781 ccggaatcgg ctttggcggc accggctacg gcgtcagctt cagcgtgccc ctttttctgc
      841 gctatggctt cgaaggcgag gtggcacagg cggaggcgca gctcacttcg gcgatggccg
      901 cacgggaccg ccaagtcgtc atcgccttgg ccgaggtgcg gcgcgcgcgc aatgacgtcg
      961 tctccgctgc ggagcgaagg cgccggctga ccgaagaatt gctgccgcag gccaagcgcg
     1021 ccatggacgc ggcggagttc gcctaccagc gcgg
//
LOCUS       DASRVO010000666         1250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_227666, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000666 DASRVO010000000
VERSION     DASRVO010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227666"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFF05_08225"
     CDS             complement(<1..629)
                     /locus_tag="VFF05_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HZW51833.1"
                     /translation="MNDEASSTAVLAGLSESEAQARLRDEGYNELPRAGRRTPFRIIL
                     EVMREPMLALLAGGGVVYLALGDLQEALILLAFAMVSIGIVQESRSERVLESLRSLAS
                     PRALVIRDSVRKRIAGREVVRGDLVVLSEGDRVPADAVLLQATDLQADESLLTGESVP
                     VRKVPRMAMATAARPGGDDLPFMFSGALVVRGAGIAEVTATGGRSEIGK"
     gene            complement(751..1137)
                     /locus_tag="VFF05_08230"
     CDS             complement(751..1137)
                     /locus_tag="VFF05_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HZW51834.1"
                     /translation="MGYYFAKTLDIGFDHALRHTTEALKQEGFGIITEIDVKETLKKK
                     INVDFRNYRILGACNPALAHKALEIEDKVGTMLPCNVVVQDLGQGRVEVAAIDPVASM
                     LAIDNPRLKAAAEEVRAKLRKVIESL"
BASE COUNT          230 a          439 c          363 g          218 t
ORIGIN      
        1 atcttgccga tctcgctgcg cccgccggtc gcggtcacct cggcgatgcc ggcgccgcgc
       61 accaccagcg cgccggagaa catgaagggc agatcatcgc cgcctggccg cgccgccgtg
      121 gccatagcca tccgcggcac cttgcgcacc ggcacggatt cgccggtcag cagcgattca
      181 tcggcttgca ggtcggtcgc ctgcaacagc acggcatcgg ccggaacacg gtcgccttcg
      241 gacaggacga ccaaatcccc gcgcaccacc tcgcggccgg cgatgcgctt gcgcacgctg
      301 tcgcggatga cgagcgcgcg cgggctggcg agactgcgca gggattccag aacccgttcg
      361 ctgcgcgatt cctggacgat gccgatggag accatggcga aagcgagcag gatcagcgcc
      421 tcctgcaaat cgcccaaggc gagatagacc accccgccgc ccgccaacag cgccagcatc
      481 ggctcgcgca tgacttcaag gatgatgcga aagggcgtgc ggcgcccggc gcgcggcagc
      541 tcgttgtaac cctcgtccct gagccgggcc tgcgcctcgc tttcgctcag gcctgcaaga
      601 acagcagtgg atgaagcctc gtcattcata agcgtccggt caggttaatg gcggagagcc
      661 gtcaggcccc ccacgccggc tgccatgatg acatccgcgc gcgtgagtcc caaacgccga
      721 aacggccggg cgcttctggc aaacgcgccc tcaaagcgac tcaatgacct tgcgcagctt
      781 ggcgcgcacc tcttccgccg ctgctttgag ccgcgggttg tcgatcgcca gcatggacgc
      841 aacaggatcg atcgccgcca cttcaacccg gccctgcccg agatcctgca ctacgacgtt
      901 acacggcaac atcgtgccaa ccttgtcctc gatctccagc gctttgtgcg ccagcgccgg
      961 attgcacgcg cccaggatgc ggtagtttcg gaaatcgaca ttgattttct tcttgagcgt
     1021 ctcctttacg tcgatctcgg tgatgatgcc gaaaccttcc tgcttcagcg cttcggtcgt
     1081 atggcgcagc gcgtgatcga aaccgatgtc gagagtcttc gcaaaataat agcccatggg
     1141 aatcctcctc tcttaatcat gcatcaccgg atcaaagacg tcttgaccga gatcgagccg
     1201 cctatctcca acgctttcgc aaccttccgt tcccgcagcc gccccggcgg
//
LOCUS       DASRVO010000667         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_71767, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000667 DASRVO010000000
VERSION     DASRVO010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71767"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..>1671
                     /locus_tag="VFF05_08235"
     CDS             242..>1671
                     /locus_tag="VFF05_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZW51835.1"
                     /translation="MSTSIKPETSAVAAIAELPVLPLRDVVVYPHTVLPLFVGREKSM
                     KALDMTMDGDKRILLVAQRGADIDDPKAGDLYSIGTVASILQLLKLPDGTVKVLVEGS
                     TRAEVVRYGERDGVMTGHVRTLDPAYTRAPREIEVVARTVVSQFENFIKLNRKIPPEL
                     LTTLAGIDDASRLADTLAHHLSIRLSDKQRLLETPDVADRLEMLIGFVEGEIDVQQIE
                     KRIRGRVKTQMEKSQREYYLNEQMKAIQKELGENEEVPNEVEELGKKIEKAGMPKPVE
                     KKARAELNKLKQMSPMSAEATVVRNYIDWLVGAPWRKRSKVRKNLKAAEDVLDTDHYG
                     LEKVKERIVEYLAVQQRVKKMKAPILCLVGPPGVGKTSLGQSIARATNRKFVRMSLGG
                     VRDEAEIRGHRRTYIGSMPGRIIQNMSKVGTRNPLFVLDEIDKMSMDFRGDPSSALLE
                     VLDPEQNHTFNDHYLEVDLDLSEVMWV"
BASE COUNT          349 a          523 c          528 g          271 t
ORIGIN      
        1 ccctgttcgc ggcgaatgcg cgtcgcgaca agtcgcgtag cgtcgcccag cgtgcggtcg
       61 atgccgattc cgcggatctt gcggatgatg cgattgccgc gcgcgtgccg aaatcctgac
      121 aacgccgcgc tgcgcatcat ctccaccttg aaatcctgtc gccgcgtcgt cacttgacga
      181 acatcgcgca tgcgctcacg ctggccgtga accgcctgcc accccatcga caggaaacgc
      241 aatgagcacc agcatcaagc ccgaaacgtc cgccgttgcc gccatcgccg aattaccggt
      301 gctgccgctg cgcgacgtcg tggtctatcc gcacacggtg ttgcctttgt tcgtcggccg
      361 cgaaaagtcg atgaaggcgc tcgacatgac catggatggc gacaagcgca tcctgctggt
      421 ggcgcagcgc ggcgcggaca tcgacgatcc caaggcgggc gatctgtatt ccatcggcac
      481 ggtcgccagc atcctgcaat tgctcaagct gccggatggc acggtcaagg tgctggtcga
      541 gggcagtacg cgcgcggaag tcgtgcgcta cggcgaacgc gacggcgtca tgaccggcca
      601 cgtgcgcacg ctcgatccgg cctacacgcg cgcgccgcgc gagattgaag tcgtcgcgcg
      661 cacggtagtg tcgcagttcg agaacttcat caagctcaac cgcaagattc cgccggagtt
      721 gctgaccacg ctggccggca tcgacgatgc atcgcgcctt gccgacaccc tcgcgcacca
      781 tctttcgatc cgtctgtccg acaagcagcg cctgctggag acgcccgacg tggccgatcg
      841 cctggaaatg ctgatcggat tcgtcgaggg cgagatcgac gtgcagcaga tcgaaaaacg
      901 catccgcggc cgcgtcaaga cgcagatgga gaagagccag cgcgagtact acctcaacga
      961 gcagatgaag gcgatccaga aggaactggg cgagaacgag gaagtgccga acgaggtcga
     1021 ggagctcggc aagaaaatcg aaaaggccgg catgccgaaa ccggtcgaga agaaggcgcg
     1081 cgcggagctc aacaagctca agcagatgtc gccgatgtcg gccgaggcca ccgtcgtgcg
     1141 caattacatc gactggctgg tcggcgcgcc atggcgcaag cgcagcaagg tgcgcaagaa
     1201 cctgaaagcc gccgaggatg tactcgacac cgatcactac ggcctggaaa aggtcaagga
     1261 gcgcatcgtc gaatacttgg ccgtgcagca gcgggtgaag aagatgaagg ccccgatcct
     1321 gtgcctggtc ggtccgccgg gcgtgggcaa gacctcgctc gggcagtcca tcgcgcgcgc
     1381 cacgaaccgc aagttcgtgc gcatgtcgct gggcggcgtg cgcgacgaag ccgagattcg
     1441 cggccaccga cgcacctaca tcggctcgat gccgggccgc atcatccaga acatgtcgaa
     1501 ggtcggcacg cgcaatccgt tgttcgtgct cgacgaaatc gacaagatgt cgatggattt
     1561 tcgaggcgat ccgtcctcgg cgctgctcga agtactcgat cccgagcaga atcatacgtt
     1621 caacgatcac tacctcgagg tcgaccttga tctgtccgag gtgatgtggg t
//
LOCUS       DASRVO010000668         6573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_148540, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000668 DASRVO010000000
VERSION     DASRVO010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148540"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFF05_08240"
     CDS             complement(<1..317)
                     /locus_tag="VFF05_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZW51836.1"
                     /translation="MLKKLPAICAGLLLTLTAYAASVQLAKDHPDTYTVRKGDTLWSI
                     AAKFLQKPWQWPEVWQANPQVRNPHLIYPGDVLSLAYLNGKPGVGFAGRGGIEPHTRA
                     EQL"
     gene            525..1022
                     /gene="def"
                     /locus_tag="VFF05_08245"
     CDS             525..1022
                     /gene="def"
                     /locus_tag="VFF05_08245"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZW51837.1"
                     /translation="MLTILEFPDPRLRTKAQPVVVFDKALHTLIDAMFATMYAAPGIG
                     LAATQVNVHKQLLVLDVSEEKDAPLALINPKIVERVGAQVYQEGCLSVPGIFADVERS
                     DRIVVEAQDRNGQPVHIEADGLLAVCIQHEMDHLIGKLFVDYLSPLKREMVRKKLEKA
                     HRATA"
     gene            1030..1962
                     /gene="fmt"
                     /locus_tag="VFF05_08250"
     CDS             1030..1962
                     /gene="fmt"
                     /locus_tag="VFF05_08250"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZW51838.1"
                     /translation="MPAPLRLVFAGTPEFAAPHLRACLAENVEIAAVYTQPDRPAGRG
                     RTLAASPVKQAALAAGLVVEQPESLKSPDAKARLRELNPDLLVVVAYGLILPRAVLAV
                     PRLGCWNVHASLLPRWRGAAPIQRAILAGDTQTGVCLMRMEAGLDTGPVMLRREIDIA
                     ADDTGGSLHDKLAMLGAQTLGEGLRRTLAGETLTALPQPEAGITYAHKLEKSEARLDW
                     NESAIVLERKVRAFNPWPVAEADLAGERARVWGAQAIPGNGASPGAVIAATKHGIDVA
                     CGEGVLRVTRLQRAGGRVVTAADYLNARPELKRA"
     gene            1959..3263
                     /gene="rsmB"
                     /locus_tag="VFF05_08255"
     CDS             1959..3263
                     /gene="rsmB"
                     /locus_tag="VFF05_08255"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZW51839.1"
                     /translation="MSACRPDPRALAAQALASVVAGQSLRAAFAALSTKLHEARDRAL
                     LSSLLHEGARWWLRFDTALGSLLERPLRERESDIHALLVLGLVQLEIMGLSDYAAVAA
                     TVEATRALRKPAFAGLVNAVLRRWLRERETRRVELDRDAVTRTAHPRWLIERFDADWP
                     EQAEAILTVNNAPASLWLRVNRRRVMRADFAAQLAEQGVVCRVPDDAPDALLLDASRD
                     VTTLPGYAQGLFSVQDGAAQQAARLLDLHDGQHVLDACAAPGGKSAHILETADVELTA
                     LDSDARRLPRLRENLTRLGLSAEVRVGDAADPHAWWDNRPFDRILVDAPCSATGIIRR
                     QPDVKLHRRAADIPALATIQSRLLQALWPLLRSGGRLVYATCSLLADENARQIDAFLA
                     RHADACAIAPALPWHAVGAGVQNLPGESGMDGFFYAVLEKHN"
     gene            3238..3759
                     /locus_tag="VFF05_08260"
     CDS             3238..3759
                     /locus_tag="VFF05_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HZW51840.1"
                     /translation="MRYWKSIISIFSCIALGACAPAPGAFVVRAAALDAGTLTVRSDW
                     QPDAQVLDALDHGIELPFVVTLSAQRPGFASTRIRRHLQLRYFPLSRLYQVRDVENAL
                     TRSYAARSLALAAMENLRLSLPGFDGAGATQLRVRIALDRDELPGALRLPAVLRSAWH
                     MDSGDYAWLPRAG"
     gene            3738..5891
                     /locus_tag="VFF05_08265"
     CDS             3738..5891
                     /locus_tag="VFF05_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW51841.1"
                     /translation="MAPAGRLTRIARRALPALAVAVLLAAALVLANDAAGGASRLGAW
                     YPWMLGACVLALATLVATIVQRLLRLRRELRERAPGASLNRRVLAMLIVLALPPVFVV
                     YGFALNFLDATIDTWFNVRMEHAMDDALEIGRVYLDERLQAAQQESAALARTLHDSAD
                     ADLQKRLDAAIDAQGALQLAVFGKDQSVLATASADPQLLTPAYPDSAALLQVQGNGHY
                     AAAEPLGARLVLRVVDPLPATTPGSERLLQALYPLPARLQALTTGVEQANFDFQRLKF
                     LRSSLKLTFTLVLTFVLLLSVLFALMLAFGIARRLTAPIGRLAAATRAVGAGRYDTPL
                     PVGSRDELGFLLASFNQMQRELEQSNARLTHSAQETENQRAYLKAVLERLSAGVLGMD
                     RTGTLRTLNRAAESILDLTLARYVGQPLSVLRRDQPQLAPLLDPILQHVREGLREWRE
                     EVVLVRGDERRLLLLRGAELSGADAGIVAVFDDLTQLDRAQRDAAWAEVARRLAHEVK
                     NPLTPIQLAAERLRRRFIGRLPPDDTDVLDRATHTIVAQVEALKSLVNAFGDYARPPL
                     IESRALDLHALAGEVLDLYENDQRIQLTRRFDAHAPVLRADAGRLRQLLHNLLKNAIE
                     AIGDARKPHIEMATRDFVENEQKWIELRVADNGPGLPEGFGERWFEPYTSSKQRGTGL
                     GLAVAKKIAEEHGGTIRAEDRNGGGAIFILRLPRD"
     gene            6048..>6573
                     /locus_tag="VFF05_08270"
     CDS             6048..>6573
                     /locus_tag="VFF05_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZW51842.1"
                     /translation="MTERRQWLWFFLIALVVLAAGYGLRDPWPADEPRFVLVAKQMLQ
                     SGDWLFPHRGIELYADKPPLYFWLLAGARALVGSWRWSFLLPSLLAGLGVLWLVCDLA
                     RRLWNPRAGLYAGMAVLGAVQFVYQFKRAQIDPSLTFCTTLALYGLCRHLLLGPQWRW
                     FWIACFAAGAGVVLK"
BASE COUNT         1034 a         2193 c         2195 g         1151 t
ORIGIN      
        1 tccagctgct cggcgcgggt atgtggttcg atgccgccgc gaccggcgaa accgacgcct
       61 ggtttgccgt tcagataggc gaggctgagc acatcgcccg gatagatcag gtgcggattc
      121 cggacttgtg gattggcctg ccagacttcc ggccactgcc acggcttctg caggaacttc
      181 gccgcgatac tccacagcgt gtcccctttg cgcaccgtgt aggtgtcggg atggtccttc
      241 gcaagctgca ccgaggcggc gtaagccgtg agcgtgagca gcaatccggc acaaattgca
      301 ggtagttttt taagcatggc tgccaattac ctgattccca aagggcttcc cgagtgtata
      361 cgaaaatttt aacggcgcaa atgatggccc gcacgaaatt gccacccaga cgggcgaacc
      421 cgtgccaaag tcgcgccaag tgccgaatcg gcgtagaatt tttcccgaag tccggcactt
      481 tgccgacgct tgagccggcg ctcccgctgc cccatatcga tgccatgctg actatcctgg
      541 aattccccga cccgcgcctg cgcaccaagg cccagcccgt ggtcgttttc gacaaggccc
      601 tgcatacgct catcgatgcg atgttcgcga ccatgtacgc cgcgcccggc atcggcctcg
      661 ccgcgaccca ggtcaacgtg cacaagcaat tgctggtgct cgatgtttcc gaggaaaaag
      721 acgcaccgct ggccctgatc aacccgaaaa tcgtcgaacg cgtaggcgcg caggtctacc
      781 aggaaggctg cctgtccgta cccggcatct tcgccgacgt ggagcgttcc gatcgcatcg
      841 tcgtcgaggc gcaggatcgc aacggccagc cggttcacat cgaagccgat ggcctgctcg
      901 cggtgtgcat ccagcacgag atggatcacc tgatcggaaa gctcttcgtc gactatctgt
      961 cgccgctaaa gcgcgagatg gtcaggaaaa aattggaaaa agctcatcga gccacggcct
     1021 gagccgacaa tgcccgcgcc gcttcggctg gtttttgccg gcacacccga gttcgcggcg
     1081 ccgcatttgc gcgcctgcct cgcggaaaat gtggaaattg ctgcggtgta tacacagccc
     1141 gaccgcccgg ccgggcgcgg tcgcacgctc gcggcgagcc cggtcaagca ggccgcgctg
     1201 gcggcgggac tcgtagttga acaacccgaa tcgctcaaat cgccggatgc gaaggcgcgg
     1261 ctgcgcgagc tgaaccccga tctgctcgtc gtcgtcgcct atggattgat cctgccgcgc
     1321 gcggtgctgg cggtcccgcg cctgggttgc tggaacgtgc acgcctcgct gctgccgcgc
     1381 tggcgcggcg ccgcgccgat ccagcgcgcg atcctcgccg gcgacacgca aaccggcgtg
     1441 tgcctgatgc gtatggaggc cggactcgac accggacccg tcatgctgcg tcgcgaaatc
     1501 gatattgccg cggacgacac cggcggtagc ttgcacgaca agctcgccat gcttggtgcg
     1561 cagacgctcg gcgaaggact gcggcgcacc ctggcgggcg aaaccctgac cgcgttaccg
     1621 cagccggaag cgggcatcac ctacgcgcac aagctggaaa agtccgaggc gcggctggat
     1681 tggaacgaat ccgccatcgt gctggagcgc aaggtgcgcg ccttcaatcc atggccggtc
     1741 gcggaagcgg acctggccgg cgagcgcgcg cgcgtgtggg gcgcgcaagc cattccgggc
     1801 aacggtgcga gtccgggtgc cgtcatcgcg gccacgaagc acggcatcga tgtcgcctgc
     1861 ggcgaaggtg tgctgcgagt gaccagactc cagcgtgccg gcgggcgcgt cgtgaccgcg
     1921 gccgattatc tgaacgctcg ccccgagttg aagcgcgcgt gagcgcatgt cgccctgacc
     1981 cgcgcgcgct ggcggcacag gcgctggcca gcgtcgtcgc cggacaatcg ctgcgtgcag
     2041 catttgccgc attgtccaca aagttgcacg aggcgcgcga tcgcgcactg ttgtcgagcc
     2101 tgctgcacga gggcgcgcgc tggtggctgc gcttcgacac ggcgctggga tcgctgctgg
     2161 agcggccact gcgcgagcgc gaaagcgata tccacgcctt gctcgtactc ggcctcgtgc
     2221 aactggaaat catgggcctg agcgactacg ccgccgtggc cgccacggtc gaggccacgc
     2281 gcgccttgcg caagccggcg tttgcgggtc tggtcaacgc cgtgctgcgg cgctggctgc
     2341 gcgagcgcga gacgcgacgc gtggagctgg atcgcgatgc ggtgacgcgc accgcgcatc
     2401 cgcgctggct gatcgaaaga ttcgacgccg actggccgga gcaggccgaa gccatcctca
     2461 ctgtgaacaa cgcaccggcc tcgctgtggc tgcgcgtcaa ccgacgccgc gtaatgcgcg
     2521 cggatttcgc ggcgcaattg gcggagcagg gcgtcgtttg ccgcgtgccg gatgatgcgc
     2581 ccgacgcgtt gctcctcgat gcgagccgcg acgtgaccac cctgcccggc tacgcgcaag
     2641 gtttgttctc ggtgcaggac ggcgccgcgc aacaagccgc gcgcctgctt gacctgcatg
     2701 atggccaaca cgtgctcgat gcctgcgccg cacctggcgg caagagcgcg catattctgg
     2761 aaacggcgga tgtggagttg accgcgctgg acagcgatgc acggcgattg ccgcgcctgc
     2821 gcgagaacct gacgcgtctt ggcctgtccg ccgaggtgcg cgtgggcgac gctgccgatc
     2881 cgcacgcctg gtgggacaac cgcccgttcg atcgcattct cgtcgatgcg ccgtgttcgg
     2941 cgaccggtat catccgccgc cagccggatg tgaaattgca tcggcgcgcg gcggatattc
     3001 ccgccctggc cacgatacag tcgcgcctgc tccaggcgct gtggcctttg ctccggagcg
     3061 gcgggcgctt agtctatgcc acctgctcgc tgcttgcgga cgagaatgcg cgccagatcg
     3121 atgcgtttct cgcccgtcat gcggatgcat gcgccatcgc gccggcattg ccttggcatg
     3181 cggtgggcgc gggcgtgcag aacctgccgg gcgaatccgg catggacgga tttttttatg
     3241 cggtattgga aaagcataat tagtatattt tcgtgcatcg cgcttggcgc ctgcgcgcct
     3301 gcgcccggcg cattcgtcgt gcgcgcggcg gcgctggatg ccggcacgtt gaccgtgcgc
     3361 agcgactggc aaccggacgc gcaggtgctc gatgcgctcg accacggcat cgaactgccg
     3421 ttcgtggtga cgctgagcgc gcagcggcca ggattcgcgt cgacccggat ccggcggcac
     3481 ctgcaattgc gttatttccc gctgtcgcgc ctgtaccagg tacgcgacgt cgagaacgcg
     3541 ttgacgcgca gttacgcggc acgttcgctt gcgctggcgg cgatggaaaa tctgcgcttg
     3601 tcattgccgg gattcgacgg cgccggcgcg acgcaactgc gcgtgcgcat cgcgctcgat
     3661 cgcgacgaat tgcccggcgc gctgcgcctg cctgccgtgc tgcgctcggc atggcacatg
     3721 gacagcggag actacgcgtg gctcccgcgg gccggttgac gcgcatcgcg cggcgcgcgc
     3781 tgccggcgct ggccgtggcc gtgttgctgg ccgcggcgct ggtgctcgcc aacgatgcgg
     3841 ccggcggcgc aagccggctg ggcgcctggt acccgtggat gctcggcgcc tgcgtgctgg
     3901 cgctggcgac gctggttgcg accatcgtcc agcgcctgct gcgcctgcgc cgcgaattgc
     3961 gcgaacgcgc gcccggcgcg agtctgaacc ggcgcgtgct ggcgatgctg atcgtgctcg
     4021 cgctgccgcc ggtattcgtg gtctacggct tcgccctgaa ttttctcgac gcgactatcg
     4081 acacctggtt caacgtgcgc atggaacacg ccatggacga tgcgctggaa atcggccgcg
     4141 tctacctcga cgaacgcctg caggccgcgc agcaggaaag cgcggcgctc gcgcggaccc
     4201 tgcacgacag cgccgatgcc gatttgcaga aacgcctcga tgcggcgatc gatgcgcagg
     4261 gcgcgctgca actggcggtg ttcggcaagg accagtcagt gctcgccacg gccagtgccg
     4321 atccacaact gctcacgccc gcgtatccgg attcggcggc gttgttgcag gtgcagggca
     4381 acggccacta cgccgcggcc gaaccgctcg gtgcgcgcct ggtattgcgc gtggtcgatc
     4441 cgttgccggc gacgacaccc ggcagcgagc gcctgctgca ggcgctgtat cccttgccgg
     4501 cgcgcctgca ggcgctgacc acgggcgtgg agcaggcgaa tttcgatttc cagcgcctga
     4561 aattcctgcg cagctcgctc aagctcacct ttaccctggt gctcaccttc gtgttgctgc
     4621 tgtcggtgtt gttcgcgctg atgctggcgt tcggcatcgc acgccggctg acggcgccga
     4681 tcggccgcct cgccgccgca acgcgcgcgg tcggcgcggg ccgctatgac acgccgttgc
     4741 cggtcggcag ccgcgacgaa ctcggcttcc tgctcgcctc gttcaaccag atgcagcgcg
     4801 agctggaaca atccaacgcg cgcctgaccc acagcgcgca ggaaaccgag aaccagcgtg
     4861 cgtatctcaa ggccgtgctc gagcgcctgt ccgccggcgt gctcggcatg gatcgcacgg
     4921 gcacactgcg cacgctcaac cgcgccgccg aaagcattct cgacctgacg ctggcgcgtt
     4981 atgtcggcca gccgctttcc gtgttgcgcc gcgatcagcc gcaactggcg ccgctgctcg
     5041 acccgatcct gcagcatgtc cgcgaaggcc tgcgcgaatg gcgcgaggaa gtggtgctgg
     5101 tgcgcggcga cgaacgccgc ctgctcttgc tgcgcggcgc ggaactgtcc ggcgccgatg
     5161 ccggcatcgt cgccgtgttc gacgacctga cccagctcga tcgcgcccag cgcgacgcgg
     5221 cctgggccga ggttgcgcgc cgcctcgcgc acgaggtgaa gaatccgctc acgccgatcc
     5281 agctcgccgc cgaacgcctg cgccggcgtt tcatcggccg cctgccgccg gacgacaccg
     5341 acgtgctcga ccgcgccacg cacaccatcg tcgcccaggt cgaggcgctc aagtccctgg
     5401 tcaacgcgtt cggcgactat gcgcgaccgc cgttgatcga gtcgcgcgcg ctggatttgc
     5461 atgcgctcgc tggcgaagtt ctcgatctgt acgagaacga ccagcgcatc cagttgacgc
     5521 gacgcttcga tgcgcacgcg cccgttttgc gcgccgacgc cggccgcctg cgccagttgc
     5581 tgcacaacct gctcaagaac gcgattgagg cgatcggcga cgcgcgcaag ccgcacatcg
     5641 agatggcgac gcgcgacttt gtggaaaatg agcaaaagtg gatagagctg agagtcgccg
     5701 acaacggccc cgggctgccc gaaggtttcg gcgaacgctg gttcgagccg tatacctcga
     5761 gcaagcagcg tggtacgggc ctgggccttg cggtcgcaaa aaaaatcgcc gaagagcacg
     5821 gcggcacgat ccgcgcggag gatcgcaacg gcggcggagc gattttcatt ctgcgcctgc
     5881 cgcgcgattg agggaacctt gcattggctg ctgcgctcgg catctcgcat gccggcgatg
     5941 ctcggaatgc tcatgtacta gcgtgtacac tgcgcttcct gcgctccggc ggcacgcaat
     6001 ctgccttcgc tcgcgacgcc aatgcagagg tacctgagcg gattagaatg accgaacgcc
     6061 gccaatggct gtggtttttc ctgattgccc ttgtcgtgct ggcggccggc tacggcctgc
     6121 gcgatccatg gccggcggac gaacctcgct tcgtgctggt ggcgaagcag atgctgcaaa
     6181 gcggcgactg gctgtttccg catcgcggca tcgaactgta cgcggacaag ccgccgctgt
     6241 atttctggtt gctggccggc gcacgcgcgc ttgtcggcag ttggcgctgg agtttcctgc
     6301 tgccatccct gctcgcggga ctgggcgtgc tgtggctggt gtgcgatctc gcccgccgcc
     6361 tgtggaatcc gcgcgccgga ctttacgccg gcatggccgt gctcggcgca gtgcagttcg
     6421 tctaccagtt caagcgcgcg cagatcgacc ccagcctgac gttttgcacc acgctggcct
     6481 tgtacggatt gtgccggcat ctgctgctcg ggccacagtg gcgctggttc tggatcgcct
     6541 gtttcgccgc cggagcaggc gtggtgctca agg
//
LOCUS       DASRVO010000669         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_96990, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000669 DASRVO010000000
VERSION     DASRVO010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96990"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VFF05_08275"
     CDS             <1..848
                     /locus_tag="VFF05_08275"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HZW51843.1"
                     /translation="NIVPWQLVCERTGATLKAAPIAPSGELIFERFVEMLTPRVKLAG
                     VVHVSNVLGTVNPIREIARAAKNRGVPLLVDGSQAAPHMPVDVQALGCDFYALTGHKL
                     FGPTGTGMLWARKEHLAAMPPFFGGGEMIREVRIEKTTFADPPHKFEAGTPNIAGVVG
                     LGAALDYVEAVGRERIAAYEQDLLGYATAALKSVPGLRLFGEAPHKAAVLSFLIDGAH
                     AHDLATLLDHEGVAVRSGHHCAHPLMQFFGVPATLRASLSFYNTRAEVDAFVDAIARV
                     RKMLG"
BASE COUNT          161 a          337 c          344 g          178 t
ORIGIN      
        1 ccaacatcgt gccgtggcag ctcgtgtgcg agcgcaccgg cgcgacgctg aaggctgcgc
       61 cgatcgcgcc gagcggcgag ttgatcttcg agcgcttcgt ggagatgctc acgccgcgcg
      121 tgaagctcgc cggtgtggtg catgtttcga acgtgctcgg cacggtcaac ccgatccgtg
      181 aaattgcgcg cgcggcaaag aatcgaggcg tcccgttgct cgtggacggt tcgcaggccg
      241 caccgcacat gcccgtcgac gtgcaggcgc tgggctgcga tttctacgcg ttgaccggac
      301 acaagctgtt cgggccgacc ggcaccggca tgctgtgggc gcgcaaggaa cacctggccg
      361 cgatgccgcc gttcttcggc ggcggcgaga tgatccgcga agtgcgcatc gagaaaacca
      421 ccttcgccga tccgccgcac aagttcgagg cgggcacgcc gaacatcgcc ggcgtcgtcg
      481 gccttggcgc cgcattggat tatgtcgagg ctgtcggtcg cgaacgcatc gccgcctacg
      541 aacaggactt gctcggctac gccacggccg cgctgaaatc cgtgcccggc ctgcgcctgt
      601 tcggcgaagc gccgcacaag gccgcggtgc tgtcgttcct gatcgacggc gcgcatgcgc
      661 acgatctggc caccttgctc gaccatgaag gcgtggcggt gcgctccgga catcactgcg
      721 cgcatccgct gatgcaattc ttcggcgtgc cggcgacgct gcgcgcgtcc ttgtcgttct
      781 acaacacgcg cgccgaagtc gacgccttcg tcgatgcgat cgcgcgcgtg cgcaagatgc
      841 tgggatagac acagatggga ttgacggaat tggacttgaa cgcaacgccg ctcgcgttcc
      901 gcgctgcagg cgcggcggat attcctgcca tcgtggccct ggtcgaatcc gcctatcgcg
      961 gcgactccgg ccgccgcggc tggaccacgg aatcggattt gctcgatggc cagcgcgtgg
//
LOCUS       DASRVO010000670         5413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_148639, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000670 DASRVO010000000
VERSION     DASRVO010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148639"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..561)
                     /locus_tag="VFF05_08280"
     CDS             complement(214..561)
                     /locus_tag="VFF05_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HZW51844.1"
                     /translation="MSHEPDSTNTSRRRFFAVAGSAIGAAAVISVLPRQARAADLPHL
                     EASDATAQALHYNDDATKVDKAAAPTWKAGEACNNCNFFQGGANDQWGPCQLFPGKAV
                     HRAGWCAGYAKKA"
     gene            complement(641..1099)
                     /gene="ccmE"
                     /locus_tag="VFF05_08285"
     CDS             complement(641..1099)
                     /gene="ccmE"
                     /locus_tag="VFF05_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZW51845.1"
                     /translation="MTPTRKRRLIAVSLILIAVAVAAALTTLALSRNMSYLFSPSEVL
                     AGKAPEGASFRLGGVVLEHSIKRAPGSLTVDFIVTDRFHEMPVQYTGVLPDLFREGQS
                     IIATGQMQGDRFAAREVLAKHDETYMPKEVADAIAKAKAAEAAQHAGNPH"
     gene            complement(1096..1266)
                     /gene="ccmD"
                     /locus_tag="VFF05_08290"
     CDS             complement(1096..1266)
                     /gene="ccmD"
                     /locus_tag="VFF05_08290"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017155059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HZW51846.1"
                     /translation="MTDFFAMHGYAAYVWPSYALFAVVLLADALAPVFARRRALRDIA
                     ARLRRAASRKPS"
     gene            complement(1259..2020)
                     /locus_tag="VFF05_08295"
     CDS             complement(1259..2020)
                     /locus_tag="VFF05_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008207981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HZW51847.1"
                     /translation="MNPLVLWFHKLGSPPYFYRFAGKWLPWLYALALIAGVVGFYGAL
                     FTAPADYQQGDSFRIMYVHVPCAWMGLFAYLFMAANGFVGLVWRIKLSETLAMASAPV
                     GAAFTFITLVTGSLWGKPTWGTWWTWDARLTSELVLLFLYFGVIGLYHAIEDRRQAAR
                     AAAFLALIGIVNLPIVHYSVIWWNTLHQGQTVSLVGPSKIAPDMLWPLLVLAFATHAY
                     FFASVLARSRVALLELESGKDWVRALLHAAETLHD"
     gene            complement(2022..2705)
                     /gene="ccmB"
                     /locus_tag="VFF05_08300"
     CDS             complement(2022..2705)
                     /gene="ccmB"
                     /locus_tag="VFF05_08300"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZW51848.1"
                     /translation="MKPGSTSAACVALLRRDLLLVWRRRGDALNPLLFAIIVVALFPF
                     ALGPEAAVLSRIAAGTIFVAVLLAMLLSLDALFRSDLEDGSLEQLVLSPHPLALLLGC
                     KILVHWLTTALPLIVIAPLLAQLLLLPAAVLPVLLTALALATPLLSLIGAVCVALTVG
                     MRRSGMLLTLLVLPLIVPALIFGAGACNAAQNGFAYSAPLLWLAAWLVLAVILAPLAA
                     AAALKISLT"
     gene            complement(2702..3361)
                     /gene="ccmA"
                     /locus_tag="VFF05_08305"
     CDS             complement(2702..3361)
                     /gene="ccmA"
                     /locus_tag="VFF05_08305"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020684081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HZW51849.1"
                     /translation="MSSIQTEPAPSESAPLLQAQSLRFARNDEAIFGPLDFSLHAGET
                     VLIEGDNGSGKTTLLKVLSGLLEATSGNVLLHGAPLTLAKLSHQVALLGHLLGLKLEL
                     SALENLRFATGFGGIRPGITPHLALASVGLEGFEDAPLRTLSAGQRKRVALARLLLVP
                     AALWLLDEPYANLDRGGIDLVNRLLEHHARRGGAALITSHGAYAFTSGTPRRLALKAA
                     A"
     gene            3425..>5413
                     /locus_tag="VFF05_08310"
     CDS             3425..>5413
                     /locus_tag="VFF05_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZW51850.1"
                     /translation="MRFRFALLAALFPLVAAAAPAADGRHTWLVRLQGLSLVEHVHAR
                     VAAQNLAAVEGGEKPAMRRELRSVLAQDYLQQLDAARTRVLDAGSAELGRRLTPRAVY
                     RYAGNGMALSLTDAEAAHLATLPGVLGVRRERISHPLTDAGPEWIGADKLWNGQVGGV
                     AATKGEGVVIGIIDTGISPSHPSFAATGPDGYAIQNPRGHFYGLCASAQATCNNKLIG
                     IYDFTDEGTKGIDSVGHGTHVSGIAAGDAMNNALKGTTVSLSRFVSGVAPHANIIMYK
                     ACNSSSSGGTCPESALFGALDQAIADNVDVINYSIGGDAQDAWQMLAQGNTDAAAMFN
                     ARAAGIVVVAAAGNEGPGPNSLGEPGNVPWVISVANATHDREFANSIGGFSGAPNAPA
                     TQIGAGYTSGYGPAEIVYAGNYGNALCGIGPTDYPPTGKSNPFPPGTFHGQIVICDRG
                     IYARVEKGYNVKLGGAGGYILANAQSDGESIVSDDHYLPAVHLGYTEGVALKTWVASS
                     GSHEGKISGVSALLQASLGDILNSSSSRGPYGFGGGILKPDITAPGTNILSSDYQSDG
                     LVFMSGTSMASPNVAGAAALLVAAHPTWGPAQIESALLGTALAGSVRLQDGVTPASPL
                     DAGTGRVQVAAAVRAGLYLPISTADFRAQDPSRGGNPVK"
BASE COUNT         1000 a         1697 c         1884 g          832 t
ORIGIN      
        1 cgaccaccgg acgcgcgatg gagacgagcg tcgcattgcc gcgccaggcg ccgtagatcg
       61 gcaacgtgca ttgcacggcc gcgagcagca gggcgaggat gagggcgaat tggccgagtt
      121 cgggaagcat gcgggaaaga ccaacgcgct accggcttgc ggggcaagcc ggtagattac
      181 gcgagacggg gaaagattgc gacgcgcgca gcgtcaggcc ttcttcgcgt agccggcgca
      241 ccatccggct cggtgcacgg ccttgccggg gaacagctgg cacggacccc actggtcgtt
      301 cgcgccgccc tggaagaagt tgcaattgtt gcaggcctcg ccggctttcc acgtcggtgc
      361 ggcggctttg tcgaccttgg tcgcgtcgtc gttgtaatgc agcgcctgcg cggtcgcatc
      421 ggaagcttcc agatgcggca agtcggcggc gcgcgcctgg cgcggcagca cgctgatgac
      481 ggcagcggcg ccgatcgcgc tgccggcgac ggcgaagaag cggcggcggc tggtattggt
      541 ggaatcgggt tcgtgcgaca tgatcgatct ccttttgctt gaactgcgaa cgattataga
      601 cctctggtgc cgcaagcgcg tgcactaggc ttgacctgaa tcaatgcgga ttcccggcgt
      661 gctgcgccgc ttcggccgcc tttgccttcg cgatcgcgtc tgcgacttcc ttgggcatgt
      721 aggtttcgtc gtgcttggcc aggacttcgc gcgcggcgaa acgatcgccc tgcatttgtc
      781 cggtggcgat gatgctctgg ccttcgcgga acaggtccgg cagcacgccg gtgtattgca
      841 ccggcatctc gtggaagcga tcggtgacga tgaaatccac ggtcagcgat cccggcgcgc
      901 gcttgatcga atgctcgagc accacgccgc caaggcggaa gctcgcgcct tccggcgcct
      961 tgccggcgag tacttcgctt ggcgagaaca ggtagctcat gttgcggctg agcgcgagcg
     1021 tggtcagcgc ggcggcgacg gcaacggcaa tcaggatcag gctcaccgcg atcaggcggc
     1081 gcttgcgggt gggcgtcacg atggtttcct cgaagcggcg cgacgcaggc gcgcggcaat
     1141 gtcgcgcagc gcgcggcggc gcgcgaacac gggcgcgagc gcgtcggcga gcagcacgac
     1201 ggcgaataac gcataggacg gccagacgta ggcggcatag ccgtgcatgg cgaagaaatc
     1261 agtcatggag cgtctccgcg gcgtgcagca gcgcgcgcac ccaatccttg ccggattcga
     1321 gttcgagcag ggccacgcgc gaacgcgcca gtacgctggc gaagaagtac gcgtgggtcg
     1381 cgaaggcgag cacgagcagc ggccagagca tgtccggcgc gatcttcgac gggccgacca
     1441 ggctcacggt ctggccctgg tgcagcgtgt tccaccagat caccgagtag tgcacgatgg
     1501 gcaggttgac gatgccgatc agggcgagga atgccgccgc gcgcgcggcc tggcggcgat
     1561 cctcgatcgc gtggtacagg ccgatcacgc cgaaatacag gaacagcagc acgagttcgg
     1621 aagtcagtcg cgcgtcccag gtccaccacg ttccccaggt cggcttgccc cacagcgaac
     1681 cggtaacgag ggtgatgaac gtgaacgcgg cgccgacggg cgcgctggcc atggcgaggg
     1741 tttccgacaa tttgatgcgc cacacaaggc cgacaaatcc attcgcggcc atgaacaggt
     1801 aggcgaacag gcccatccac gcgcagggca cgtgcacgta catgatgcgg aaggaatcgc
     1861 cttgctggta atccgccggc gcggtgaaca gcgcgccgta gaaaccgacc acgccggcga
     1921 tcagcgcgag cgcatacagc cacggcagcc atttccccgc gaagcggtag aaatagggcg
     1981 gtgagccgag tttgtggaac cagaggacga gtggattcat ttcacgtcaa cgaaatcttc
     2041 aatgcggcgg cggcggcgag tggtgcgagg ataactgcca gcaccagcca tgcggccagc
     2101 cacagcagcg gcgcggaata ggcaaatcca ttctgggcgg cgttgcaggc gccagcgccg
     2161 aagatcagtg cgggcacgat cagcggcagc acgagcaagg tcaacagcat accagagcgg
     2221 cgcatgccaa cggtgagcgc gacgcacacc gcgccgatca aactcagcag cggcgtggcc
     2281 agcgcgagcg cagtgagcag cacgggcaaa accgctgcgg gcagcaagag cagttgcgcg
     2341 agcaggggtg cgatgacgat gagcggcaac gccgtcgtca gccagtgcac gaggatcttg
     2401 cagccgagca gcagcgccag cggatgcggt gaaagcacga gttgttccag cgagccgtct
     2461 tccaggtcgc tgcggaacag cgcgtccagc gacaacagca tggcgagcag cacggcgacg
     2521 aagatggtgc cggcggcaat gcgactgagc accgcagctt ccggaccgag cgcgaatgga
     2581 aacagcgcga ccacgatgat cgcgaacaat aaaggattca gggcatcgcc gcgccggcgc
     2641 cagaccagca gcaggtcgcg gcgcagcagc gcgacgcagg cggcggatgt cgatcccggc
     2701 ttcatgcggc cgccttcaac gcgagccggc gcggcgtgcc cgaggtgaac gcatacgcgc
     2761 cgtgcgaggt gatcagggcc gcgccgccgc ggcgcgcgtg atgctcgagc aggcggttga
     2821 cgaggtcgat gccgccgcgg tcgaggtttg cgtaaggctc gtcgagcagc cacagcgccg
     2881 cgggcacgag cagcaggcgc gcgagcgcga cacgcttgcg ttggccggcg gaaagggtgc
     2941 gcagcggcgc gtcttcgaat ccttcgaggc cgacgctggc gagcgccagg tgcggcgtga
     3001 tgccgggacg gatgccgcca aaaccggtcg cgaagcgcag gttttccaag gcggacaatt
     3061 cgagtttcaa tccgagcaaa tggccgagca gggcgacctg gtgcgagagc ttggcgagcg
     3121 tgagcggcgc accgtgcaac aacacgtttc cggatgtcgc ctcgagcaga ccggacaaca
     3181 ccttgagcag cgtggtcttg ccggaaccat tgtcgccttc gatcagaacc gtctcgcccg
     3241 cgtgcaggga aaaatccagc ggaccgaaga tcgcttcgtc gttgcgcgcg aagcgcagcg
     3301 actgcgcctg cagcaagggt gctgattctg atggtgcagg ttcggtctgg atggacgaca
     3361 taggccgcgc atcgtaaccg cacggcgcgg agtaaactag cgcgaagcct tcgtggagcc
     3421 tttcatgcgc tttcgtttcg ccctgctcgc tgcgttgttc ccgctggtcg ccgctgcggc
     3481 gccggctgcc gatggccgcc atacctggct ggtgcgactg cagggcctat cgctggtcga
     3541 gcacgtgcat gcgcgggtgg cggcacagaa cctggcggcc gtggaaggcg gcgaaaagcc
     3601 ggcgatgcgc cgcgaactgc gctctgtgct ggcacaggat tatctgcagc agctcgacgc
     3661 cgcgcgcacg cgcgtgctcg acgcgggcag tgccgaactg ggccgcaggc tgacgccgcg
     3721 tgcggtctat cgctatgcgg gcaatggcat ggcgttgtcc ctgaccgacg cggaggccgc
     3781 gcacctggcg acgttaccgg gcgtgctcgg cgtgcgccgc gaacgcatct cgcatccgtt
     3841 gaccgatgcc ggcccggagt ggatcggcgc cgacaaattg tggaatggcc aggtcggcgg
     3901 cgttgccgcg accaagggcg agggcgtggt catcggcatc atcgacaccg gcatcagtcc
     3961 ttcgcatccc tcgttcgcgg cgaccggtcc ggacggctac gcgatccaga atccgcgcgg
     4021 acatttctac ggcctgtgcg cgagcgcgca ggcgacctgc aacaacaagc tgatcggcat
     4081 ctacgatttc accgacgagg gcaccaaggg tatcgacagc gtcggccacg gcacgcatgt
     4141 ttccggcatc gccgccggcg acgccatgaa caacgcgctg aagggcacga cggtgtcgct
     4201 gagccgcttc gtgtcgggcg tcgcgccgca cgccaacatc atcatgtaca aggcctgcaa
     4261 ttccagcagc tccggcggca cttgtccgga atcggcgttg ttcggggcac tggatcaggc
     4321 catcgccgac aacgtggacg tcatcaacta ttccatcggc ggcgatgcgc aggacgcctg
     4381 gcaaatgctc gcacagggca acaccgacgc ggcggcgatg ttcaacgcgc gcgcagcagg
     4441 catcgttgtc gtggcagcgg cgggcaatga aggtcccgga ccgaattcgc tcggcgaacc
     4501 cggcaatgtg ccgtgggtga tcagcgtggc gaatgcgacg cacgaccgcg agttcgccaa
     4561 ttccattggc ggtttttccg gtgcgccgaa tgcgccggca acccagatcg gtgccggtta
     4621 cacatccggt tatgggccgg cggaaatcgt ctacgccggc aactacggca atgccctgtg
     4681 cggcatcggg cccaccgatt atccgccaac cggcaagagc aacccgtttc cgccgggaac
     4741 attccacggc cagatcgtga tttgcgatcg cggcatctac gcgcgcgtgg aaaaaggcta
     4801 caacgtgaaa ctcggcggcg ccggcggcta catcctcgcc aacgcgcaaa gcgacggcga
     4861 atccatcgtc agcgacgatc actacctgcc ggcggtgcac ctgggctata ccgagggcgt
     4921 ggcgctcaag acgtgggttg cgtcgtccgg cagtcatgag ggcaagatca gcggcgtcag
     4981 cgcgctgttg caggcgagtc tgggcgacat cctcaattcc agcagctcgc gcgggcccta
     5041 cggtttcggc ggcggcatcc tcaagcccga catcaccgcg cccggcacga acatcctgtc
     5101 gtcggattac caatccgatg ggctcgtgtt catgtccggc acgtcgatgg catcgccgaa
     5161 tgttgccggc gcagccgcat tgctcgtcgc cgcgcatccg acctggggtc cggctcagat
     5221 cgagtccgcg ttgttgggca ccgctctggc gggaagcgta cgcttgcagg acggcgtgac
     5281 gccggcttcg ccgctggatg cgggcacagg gcgcgtgcaa gtcgccgccg ccgtgcgggc
     5341 gggactgtat ctgccgatca gcaccgcgga tttccgcgcg caggatccgt cgcgtggcgg
     5401 caatccggtc aag
//
LOCUS       DASRVO010000671         1391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_356442, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000671 DASRVO010000000
VERSION     DASRVO010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356442"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFF05_08315"
     CDS             complement(<1..642)
                     /locus_tag="VFF05_08315"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZW51851.1"
                     /translation="MPMKAAATDRLRALRVIGVALATLLICAGCAKAPPVPARSRLIV
                     LGFDGMDPNLTDKWMADGSLPHFAQLARSGHYQRLATTNPPQSPVAWASFATGANPGA
                     HGIFDFLRRDKSDYAPEFSIADTTPPQHVLHLFGWELPLDSGSLRNLRDGTPFWSTAE
                     RDGDRATVLRVPVTYPPDLITRMLSGMGVPDLLGTQGTYTIYATRPIPGAENGG"
     gene            complement(617..847)
                     /locus_tag="VFF05_08320"
     CDS             complement(617..847)
                     /locus_tag="VFF05_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51852.1"
                     /translation="MRWICFALLLCLSTGAHAYIGPGAGLNVLGSLWAVLVGIVLALF
                     AILTWPLRLLWRKLRGPRDTQREHNADESRRD"
     gene            1003..>1391
                     /locus_tag="VFF05_08325"
     CDS             1003..>1391
                     /locus_tag="VFF05_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51853.1"
                     /translation="MNARLPRFMRWLGDLETRVLDGELPALRIERPVYVCGLARSGTT
                     LLLEALAAAPDFTTHRYSDYPLLWTPYWWNWLRARLPLPAQTPVERAHRDRLTVTANS
                     PEAFEEVFWMHFFPGRHDAAVDQVIDAT"
BASE COUNT          253 a          447 c          459 g          232 t
ORIGIN      
        1 accgccattt tcggcgccag ggatcggccg cgtcgcatag atcgtgtagg tcccttgcgt
       61 gccgagcaga tcgggtacgc ccatgccgga cagcatgcgc gtgatcaggt ctgggggata
      121 agtcaccggc acgcgcagta ccgtggcgcg gtcgccgtct cgttccgccg tcgaccagaa
      181 cggcgtgccg tcgcgcagat tgcgcaggct gccggaatcc agcggaagtt cccagccgaa
      241 caagtgcaat acatgttgcg ggggcgtcgt gtcggcgatc gagaactccg gtgcgtagtc
      301 agacttgtcg cgacgcagga aatcgaagat gccgtgcgcg ccgggattgg cgcccgtggc
      361 gaagctggcc caggcgaccg gcgactgcgg cggattggtc gtggccagac gctgataatg
      421 gccggatcgg gccagttgcg cgaaatgcgg cagactgccg tccgccatcc acttgtctgt
      481 cagattggga tccatgccat cgaaaccgag cacgatcagg cgagatcggg cgggtacggg
      541 cggcgctttg gcgcagccgg cgcagattag aagggtggcc agcgccaccc cgataacgcg
      601 caacgcgcgc aatcgatcag tcgcggcggc tttcatcggc attgtgttcg cgttgcgtgt
      661 cccgcgggcc gcgcaatttg cgccacagca ggcgcagggg ccaggtcagg atggcgaaca
      721 atgccagcac gatgccgacg agcaccgccc acagcgatcc gagcacgttc aggccggcac
      781 ccggccctat gtaggcatgc gctccggtgc tcaggcacag cagcaacgca aagcagatcc
      841 aacgcatggg acttccgcga tcattaaaag gcaagtgtag ccaagacgcg cgggcgccgc
      901 gagcgcggca acattttcgc ggcacgccgt accggtaata atgggcgaat gccagcttca
      961 cgccatccac caatccctgc ctcggacaaa atcgcgggca ccttgaacgc gcgcctgccc
     1021 cgattcatgc gttggctcgg cgatctcgag acgcgcgtcc tcgatggcga attgccggcg
     1081 ctgcgtatcg agcgcccggt ctatgtgtgt ggcctggccc gatccgggac cacgctgctg
     1141 ctcgaggcgc tggctgcggc accggatttc acgacccatc gctacagcga ctatccgctg
     1201 ctgtggaccc cctattggtg gaattggctg cgcgcgcgtc taccgctgcc ggcgcaaacg
     1261 cccgtcgagc gtgcccatcg cgaccgtctc acggtcacgg cgaacagccc ggaagccttc
     1321 gaggaagtgt tctggatgca cttctttccg ggacggcacg atgccgcggt tgatcaggtc
     1381 atcgacgcca c
//
LOCUS       DASRVO010000672         1067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_148671, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000672 DASRVO010000000
VERSION     DASRVO010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148671"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFF05_08330"
     CDS             complement(<1..407)
                     /locus_tag="VFF05_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW51854.1"
                     /translation="MFTDTQRLAGLPPDLVRAREEIVNAILQQRLDAAAHGVAAALVR
                     APQHPELLRLSALVQMAMRQNEEAIASLLMARAAQPDDPLIHDALGTAYETVHDYSRA
                     RASLHRACALDPGNSGFWFNYANRLFEGGDTAGS"
     gene            complement(561..>1067)
                     /locus_tag="VFF05_08335"
     CDS             complement(561..>1067)
                     /locus_tag="VFF05_08335"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW51855.1"
                     /translation="ARWRERRPRFTDKSPDNWRFIGAVMHMLPGARVVICQRDPVETC
                     LACFRQMFVRGGQAFSYDLDDLAAYWRDFDRAARHWQTTYPDRVHMQSFEALVAHPEA
                     QIRALLEFCGLAFDPACLKFHETQRNVATASAAQVREPLRRNGARAPAYGALLSPLRA
                     ALRCDGPA"
BASE COUNT          205 a          341 c          355 g          166 t
ORIGIN      
        1 gagccggcgg tgtcgccgcc ctcaaacagg cggttggcgt aattgaacca gaaacccgaa
       61 ttgcccggat ccagtgcaca ggcacggtgc agggacgcgc gagcacgcga ataatcgtga
      121 accgtttcgt acgcggttcc cagcgcatcg tgaatcaacg gatcgtcggg ctgtgcggcg
      181 cgcgccatga gcagggacgc gatcgcctcc tcgttttgcc gcatcgccat ctggaccaaa
      241 gcggacagtc gcaacaattc cggatgctgc ggcgcacgca ctaacgccgc ggcgacgcca
      301 tgcgcagccg cgtcgagccg ctgctgcagg atcgcattca cgatttcttc tctggcacgc
      361 accagatccg gtggcagtcc tgccaggcgt tgggtgtcag tgaacatggg gcactatcgt
      421 ggcaattcgg ccggcgcgtg aacgcgccgg gtgctggcca agcaccgaag cccgtgccag
      481 caggacgcaa accaaggatg cgaaatttcc gcctgcatag tctaaacgat ccacgcgccg
      541 gcgtccgcag cgcagcgcgc tcatgcaggc ccgtcgcagc ggagggctgc gcgcagtggg
      601 ctcagcaatg caccgtaggc cggcgcgcgc gcgccgttgc ggcgtaacgg ttcgcgcacc
      661 tgcgcggcgc tggcagtggc gacgttgcgc tgggtctcgt ggaatttcag acaggccgga
      721 tcgaacgcca atccgcagaa ttcaagcaag gcacgaatct gcgcttcggg gtgggcgacg
      781 agcgcctcga acgactgcat gtgcacgcga tccgggtacg tggtctgcca atggcgggca
      841 gcgcggtcga aatcgcgcca gtacgcggcc agatcgtcca gatcgtagct gaatgcctgc
      901 ccgccacgca cgaacatctg gcgaaagcag gcgaggcagg tttccacggg gtcgcgctgg
      961 caaatcacca cgcgtgcacc cggcagcatg tgcataacgg cgccgatgaa acgccagttg
     1021 tccggcgatt tgtcggtgaa tcgaggacgc cgctcacgcc agcgcgc
//
LOCUS       DASRVO010000673         1644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_201902, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000673 DASRVO010000000
VERSION     DASRVO010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201902"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..>1644)
                     /locus_tag="VFF05_08340"
     CDS             complement(150..>1644)
                     /locus_tag="VFF05_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HZW51856.1"
                     /translation="GDVVGTPKYMAPEQAQGRNDALDARSDQCALGLILYEMVTLNPP
                     YEGRTAYEVLANAASGKRRPIVHAYLRRRIPRELRAIIERATAYKPQDRYADVVALAA
                     DVRRYLRGEAVLAQPDSVMQRAQRWIARHRQAALNAVLAIVAVAAVAIGGLLWLNQRQ
                     FNAERLREQRLLALSTNVADIGDQVQMRFLQLEGGIENLADSVSQILVHGQDSTQRVY
                     LFEDFHDAARVPLDLVPSALRQGKISLGSPVWIIPKSADRAALMAQVHRLASLQAFMR
                     DIYARSARMVERSGQDLYAGGQPQVENDASPLGAILIALDDGLTARYPGWDGLPADYD
                     PRQRPWYAIARGKHGPQWGDPYTSIGNGPVELPLSVPLYDDRQRFVGVVTAAFMPDLM
                     IKSLFVMRETNAVRAMYLLDANGHILAATGATIPLERPAQDESLHQVFPSPELLARIK
                     SGQTGVLETRLRGEPVVIAFDDVAPFGWSLAAVADPRALFKKAVAGD"
BASE COUNT          260 a          525 c          566 g          293 t
ORIGIN      
        1 ttgtggatgg cgatgatggc cttgaggccg gcgtccttgc tctggcagaa cacgacctgt
       61 tcgtggccgc tggtggcgat ggcgtcgaaa atcatgggac tctccgatag ggcagccgcg
      121 cattttagca ggatgcgcca gccggggcct caatcgccgg caacggcctt cttgaacaat
      181 gcgcgcggat cggctaccgc ggcgaggctc cagccgaagg gcgcgacatc gtcgaaggcg
      241 atcactaccg gttcgccgcg cagacgcgtt tccagcacgc ctgtctgtcc gctcttgatc
      301 cgggccagca gttcgggtga cgggaaaacc tgatgcagcg attcatcctg cgccggccgc
      361 tcgagcggta tcgtggctcc ggtcgcggcg aggatgtggc cgtttgcatc gagcaggtac
      421 atcgcgcgga ccgcgttcgt ttcgcgcatt acgaacagcg acttgatcat gaggtcgggc
      481 atgaacgctg ccgtcaccac gccgacgaag cgctggcggt cgtcgtacag gggcacgctc
      541 agcggcagtt caactggccc attgccgatg ctcgtgtagg gatctcccca ttgcggtccg
      601 tgcttgccgc gcgcaatggc gtaccacggc cgctggcgcg gatcgtaatc ggccggcagg
      661 ccgtcccagc cgggatagcg tgcggtaagt ccgtcgtcca gcgcgatcag gatcgcgccg
      721 agcggactgg cgtcgttttc cacctgcggc tggccgccgg cgtacaggtc ctgtccgctg
      781 cgttcgacca tgcgcgccga acgcgcgtag atgtcgcgca tgaaggcctg cagcgaggcg
      841 agccgatgga cttgggccat caacgcagcg cggtccgcgc ttttcgggat gatccagacc
      901 ggtgaaccga ggctgatttt tccctgccgc aacgcgctcg gcacgagatc cagcggaacg
      961 cgcgctgcat cgtggaaatc ctcgaacagg tacacgcgtt gcgtcgaatc ctgcccgtgg
     1021 accaggatct gggaaaccga atcggccagg ttttcgatgc cgccttcgag ctgcaggaaa
     1081 cgcatctgca cctggtcgcc gatgtcggca acgttcgtgc tgagcgcaag caggcgttgc
     1141 tcgcgcaggc gctcggcgtt gaactggcgc tggttgagcc agagcaggcc gccgattgct
     1201 acggcggcga cggcgacgat ggcgagcacg gcgttcaacg ccgcctggcg atggcgggcg
     1261 atccagcgct gcgcgcgttg catcaccgaa tccggttgcg ccagcaccgc ttcgccgcgc
     1321 aggtagcggc gaacgtctgc cgccagtgcg accacatcgg cgtagcggtc ctgcggcttg
     1381 tacgcggtcg cgcgttcgat gatggcgcgc agctcacgcg gaatgcgccg gcgcaggtag
     1441 gcgtgcacga tcgggcggcg cttgccggaa gcggcgttcg caagcacctc gtaggccgtg
     1501 cggccttcgt agggcggatt cagcgtgacc atctcgtaca ggatcaggcc cagcgcgcat
     1561 tggtcgctgc gcgcatcgag cgcgtcattg cggccctgcg cctgttccgg cgccatgtac
     1621 ttgggtgtgc cgaccacgtc gccc
//
LOCUS       DASRVO010000674         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_201925, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000674 DASRVO010000000
VERSION     DASRVO010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201925"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFF05_08345"
     CDS             <1..269
                     /locus_tag="VFF05_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HZW51857.1"
                     /translation="GFGARDLHILTNLSLSPAYMTSMTHVVLARDLYPERLVGDEPEE
                     LEVVEWKLSQLHHLLSHPEVTEGRSIAALFIAREYLAGRFSPRT"
     gene            266..1069
                     /gene="cysQ"
                     /locus_tag="VFF05_08350"
     CDS             266..1069
                     /gene="cysQ"
                     /locus_tag="VFF05_08350"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HZW51858.1"
                     /translation="MSESELQDLARACCALAREAGEAIMRVYAGDFAVERKDDNSPLT
                     AADLAAHRVIVAGLQALAPQIPVLSEESAEQAAWSVRQTWPRYFLVDPLDGTREFVKR
                     NGEFTVNIALIDAHATVLGVVYAPALDEMYLAWADGHAHFATKSQSGKLRTRARATPL
                     VVAGSRSHGDPRMGVALDKLGPHELLALGSSLKFCRTARGEADLYIRYGPTSEWDTAA
                     GQCVLEQAGGAVQRMDGSTLKYNTKDSLLNPDFLACGDAAVDWAGLLRG"
     gene            1080..>1864
                     /gene="mazG"
                     /locus_tag="VFF05_08355"
     CDS             1080..>1864
                     /gene="mazG"
                     /locus_tag="VFF05_08355"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZW51859.1"
                     /translation="MKSDIDDLLRIMASLRDPQHGCPWDVQQTFATIAPYTIEEAYEV
                     ADAIDRGDMADLRDELGDLLLQVVFHARMAQEQGAFDFSGVVAAICDKMVRRHPHVFA
                     GASVESAEAQLREWDAHKRREREQSGEADTSALAGIPRGLPEWQRALKLQKRASRVGF
                     DWSDPRPVLDKLAEEIGEVRHELEHGADMARLTDEIGDVLFVGVNLARLAKVDWSAAL
                     RGANAKFERRFRRMETLAAKEGRQLDGMSLAEQDAYWDRAKAE"
BASE COUNT          326 a          570 c          646 g          322 t
ORIGIN      
        1 tcggcttcgg cgcgcgcgac ctgcacatcc tcacgaacct gtcgctgtcg ccggcctaca
       61 tgacgagcat gacgcacgtg gtgctggcgc gcgacctgta tcccgagcgc ctcgtcggcg
      121 acgagccgga agaactcgaa gtggtggagt ggaagctgtc gcaactgcac cacctgcttt
      181 cgcatccgga agtgaccgag gggcgttcga tcgcggcgct gttcatcgcg cgggaatatt
      241 tggccgggcg tttttctccc cgcacatgag cgaatcggaa ttgcaggacc tggcacgcgc
      301 ctgctgcgcg ctcgcgcgcg aggcgggcga ggcgatcatg cgcgtttacg ccggcgattt
      361 cgcggtggaa cgcaaggacg acaactcgcc gctcactgcc gccgatcttg ccgcgcatcg
      421 cgtcatcgtt gccggcttgc aggcgctggc gccgcagatt ccggttttgt cggaagaatc
      481 cgccgaacag gcggcgtggt ccgtgcgtca gacctggccg cgctatttcc tggtggatcc
      541 gctcgacggc acgcgcgaat tcgtcaagcg caacggcgag ttcacggtga acatcgcctt
      601 gatcgacgcg cacgctacgg tgctcggcgt ggtttacgcg ccggcactgg atgagatgta
      661 cttggcatgg gccgatggcc atgcgcattt tgcgacaaag tcgcaaagtg gaaaattgcg
      721 tacgcgcgca cgcgcaacgc cgctcgtcgt cgccggcagc cgttcgcatg gcgatccgcg
      781 catgggcgtt gcattggaca aactcggccc gcacgaactt ctggcgctgg gttcgtcgct
      841 gaaattctgc cgcacggcgc gcggcgaagc ggatctgtat atccgctatg gcccgaccag
      901 cgagtgggac acggccgccg gtcagtgcgt gctggaacag gccggcggcg cggtgcagcg
      961 catggacggc agcacgttga aatacaacac caaggattca ctgctcaatc cggactttct
     1021 cgcctgcggc gacgcggcgg tggattgggc cggactattg cgtggctgaa tcgagattca
     1081 tgaaatcgga catcgacgat ttgttgcgca tcatggcgag cctgcgcgat ccgcagcatg
     1141 gctgtccgtg ggacgtgcag cagacattcg cgacgatcgc gccgtacacc atcgaggaag
     1201 cctacgaggt cgccgacgcc atcgaccgcg gcgacatggc cgatctgcgc gacgaactcg
     1261 gcgatctgct tctgcaagtg gtgtttcacg cgcgcatggc gcaggagcag ggcgcgttcg
     1321 acttttccgg ggtggtcgcg gcgatctgcg acaagatggt gcgtcgtcat ccgcatgtgt
     1381 tcgccggggc cagcgtggag agcgccgaag cgcaattgcg cgaatgggac gcgcacaagc
     1441 gccgcgagcg cgaacaaagc ggcgaagccg ataccagcgc gcttgccggc atcccgcgcg
     1501 gattgccgga atggcagcgc gcgctgaaat tgcagaagcg cgcctcgcgc gtgggtttcg
     1561 actggagcga tccgcgtccg gtgctggaca agcttgccga ggaaatcggc gaggtgcgcc
     1621 acgaactcga acacggtgcg gacatggcgc gcctcacgga cgagatcggc gacgtgttgt
     1681 ttgtcggcgt gaatctcgcg cgcctggcca aggtcgactg gtccgccgcg ctgcggggcg
     1741 cgaatgcgaa gttcgagcgc cgtttccggc gcatggagac gctggccgcc aaggagggcc
     1801 gtcaactcga cggcatgagc ctggccgagc aggacgccta ctgggatcgc gccaaggcgg
     1861 agga
//
LOCUS       DASRVO010000675         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_123923, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000675 DASRVO010000000
VERSION     DASRVO010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123923"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFF05_08360"
     CDS             complement(<1..542)
                     /locus_tag="VFF05_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial assembly protein"
                     /protein_id="HZW51860.1"
                     /translation="MTLTEALDLQLARLRTRYARTPLPRFFAWWGHELALLLPERWRG
                     LLAERADALLLDTAGREMVVWRQSALGCAELGRVSLDEPAETQRATFARLRGALETPD
                     LRRIYCIDGLRTLRRAVSLPVAAEDNLRQVLAFEMDRQTPFKADQVYFDYRVAPHAAA
                     ERNLHVELTVVPRAQLDAELA"
     gene            complement(539..1438)
                     /locus_tag="VFF05_08365"
     CDS             complement(539..1438)
                     /locus_tag="VFF05_08365"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51861.1"
                     /translation="MMPSRQQGVALILVIWVIALMGVLLGSFALIARTENFEARHLFD
                     GTTARYAAQAGIERAVYELRNPNLTQRWVGDGRAYDFEFDGAQVQVELTDESGLIDIN
                     TADDTLLQGLFVSAGVAPDQAVVLSDAIQDWRDPDDIPLPQGAELNEYKAAGMPYAPR
                     NAPFQTVGEVQQALGMNYDLFEKIEPAITIYGGGVQPNPAYAPLEVLLALPGMTPDLA
                     QQLIAARQQSLPGQPGGAGLTLPDGTPVVANGGGNTYTIRSRATLANGASTTLDASIR
                     LGGIGAAGQPYTVLRWDQKESSR"
     gene            complement(1435..2118)
                     /locus_tag="VFF05_08370"
     CDS             complement(1435..2118)
                     /locus_tag="VFF05_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZW51862.1"
                     /translation="MPATFKNGGFTLLEVLLAVILLGLLIAGAYSGIRAFAHAMRAGE
                     AAIDRTDRLRTAQEFLRRQISHILPLQFARDDSTGAIHVFDGNARTLRFVAPMPGYLS
                     RGGPYVQTLELAPGKDGLQLRFSDVMLNGYDPSKRSGDDPANAVVLLDHIADGRFEYR
                     ALDPDGNLADWSSDWQDPGITPLMVRVDLTMQSGAQIPWPTLDVPLMLNAGAIRPRGP
                     MLVPARGGQ"
     gene            complement(2124..>2343)
                     /locus_tag="VFF05_08375"
     CDS             complement(2124..>2343)
                     /locus_tag="VFF05_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51863.1"
                     /translation="ASAVPVAGNANAAPVQTTPIAPSIDLYKLQLDVTWGSIYLQHDA
                     RFVTLRAQNADIAQAAQMPNPARGRARQ"
BASE COUNT          384 a          771 c          796 g          392 t
ORIGIN      
        1 gccagttccg catcgagttg cgcgcgcggc accacggtca gctccacgtg cagattgcgc
       61 tcggccgcgg catgcggcgc gacgcggtaa tcgaaataca cctgatcggc cttgaacggc
      121 gtctgtcgat ccatctcgaa ggccagcacc tgacgcaggt tgtcttcggc cgcgaccggc
      181 aggctgaccg cgcggcgcaa ggtgcgcaat ccgtcaatgc agtaaatgcg gcgcaagtcc
      241 ggtgtttcca gcgcgccgcg caggcgtgcg aacgtggccc tttgcgtttc ggctggttcg
      301 tccaggctca cgcgtcccaa ctcggcgcag ccgagtgcgg attgccgcca tacgaccatc
      361 tcgcgcccgg ccgtgtcgag cagcagcgca tcggcgcgct cggcgagcag cccgcgccaa
      421 cgctccggca gcagcaacgc caactcgtgt ccccaccagg cgaaaaatcg cggtagcggc
      481 gttctggcgt agcgcgtccg caagcgggcg agctgaagat cgagcgcttc ggtgagggtc
      541 atcgggagct ttctttctga tcccagcgaa ggacggtgta cggctgccct gccgcgccga
      601 tgccgcccag ccggatgctg gcgtcgagcg tggtactggc gccgttggcg agcgtggcac
      661 gggatcggat agtgtacgtg ttcccgccgc cattggccac gaccggggtg ccgtccggca
      721 acgtcagtcc ggcgccaccg ggctggccgg gcagcgactg ctgccgtgcc gcgatcaact
      781 gctgggccag atccggcgtc atgcccggca gcgccagcaa cacctccagc ggcgcatagg
      841 ccgggttcgg ttgcacgccg ccgccataga tggtgatggc cggttcgatc ttctcgaaca
      901 ggtcgtaatt catgcccagc gcctgctgca cttcgcccac ggtctggaac ggcgcattgc
      961 gcggcgcata cggcataccg gcggccttgt actcgttgag ctcggcacct tgcggcagcg
     1021 ggatgtcgtc cggatcgcgc cagtcctgga tcgcatccga cagcacgacg gcctgatccg
     1081 gagccacgcc ggcggaaacg aaaaggcctt gcagcaaggt atcgtcagcg gtgttgatgt
     1141 cgatgaggcc ggattcgtcg gtgagctcga cctgcacctg cgcaccgtcg aattcgaaat
     1201 cgtaggcacg cccgtcaccg acccagcgct gggtcaggtt cggattgcgc aattcgtaca
     1261 ccgcgcgttc gatgccggct tgcgcggcgt agcgcgcggt ggtgccgtcg aacaggtggc
     1321 gcgcttcgaa attctcggtg cgcgcgatca gggcgaagct gccgagcagc acgcccatca
     1381 gcgcgattac ccagatcacc aggatcaggg cgactccctg ctggcgcgaa ggcatcattg
     1441 cccaccccgt gcgggaacca gcatcggccc acgcggacgg atcgcaccgg cgttgagcat
     1501 cagcggcacg tccaaggtcg gccacggtat ctgcgcaccc gactgcatgg tcagatcgac
     1561 gcgcaccatc aatggcgtga tccccggatc ctgccagtcg ctgctccagt cggcgagatt
     1621 gccgtcggga tcgagcgcac ggtattcgaa acggccgtct gcgatgtggt cgagcagcac
     1681 cacggcattg gccggatcgt cgccggatct cttcgagggg tcgtaaccat tgagcatgac
     1741 atcgctgaac cgcaattgca gaccgtcctt gccgggcgcg agttccagcg tctgcacgta
     1801 cggcccgccg cgcgacaggt aaccgggcat cggcgcgacg aaacgcaggg tgcgcgcatt
     1861 gccgtcgaac acgtggatcg cgccggtgct gtcgtcgcgc gcgaattgca gcggcagaat
     1921 gtggctgatc tggcggcgca ggaattcctg cgcggtgcgc aggcgatcgg tgcggtcgat
     1981 cgccgcctcg cccgcgcgca tggcgtgcgc gaaagcacgg atgccgctgt aggcgccggc
     2041 gatgagcagg ccgagcagga tcaccgcgag cagcacctcg agcagggtga aaccgccgtt
     2101 cttgaaagtt gcaggcatcc gcttcattgc cgggccctgc cccgcgccgg gttgggcatc
     2161 tgcgccgctt gcgcgatgtc cgcgttctgc gcgcgcaggg tgacgaagcg cgcatcgtgc
     2221 tgcaaataga tactgcccca ggtcacgtcg agctggagct tgtacaagtc gatgctgggc
     2281 gcgattggcg tggtttgcac cggcgcggcg ttggcattgc ccgccaccgg cacggcgctg
     2341 gcg
//
LOCUS       DASRVO010000676         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_253406, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000676 DASRVO010000000
VERSION     DASRVO010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253406"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..764
                     /locus_tag="VFF05_08380"
     CDS             15..764
                     /locus_tag="VFF05_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZW51864.1"
                     /translation="MRYLWRTPWVFVHVVVAVPLALVAFSPIGRTAHVRGEPLDHWLQ
                     RWWSGGIVRRCGFRIVRVGEPQPGAVLFVANHVSWLDIELMHSQRVMSFVAKAEIARW
                     PLIGWLASRAGTIFHRRGSGHSLNAVIEQAIERLRAGVAVGVFPEGGTGPGDRLRTFH
                     ARIFQIAADAQVTVQPVAISYGREGKMDLRVPFADGESFFANVVRLLGDAGVEAHVRF
                     LDPIPLSDEGRRQTAESARAAIALALGHDDA"
     gene            761..1756
                     /locus_tag="VFF05_08385"
     CDS             761..1756
                     /locus_tag="VFF05_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW51865.1"
                     /translation="MIDSDGFDPPRLLRNAHVQSVLSSSFLRRIAFRRNDAALENSAD
                     ECILDCGNGVRLQGFHSAQSVRAAPRALVVLLHGWEGSAQSNYILHTGARLLAESCDV
                     FRLNFRDHGQTHHLNRELFHSCRIDEVVGAVAEIARRFPARPLIVGGFSLGGNFALRV
                     ALRAPAAGIPLAWTFAVCPVISPRAGLAAIEDAPWFYEFYFMRKWRESLRRKQALFPE
                     RELFARGDLRGGLRDLTRRLVEQHTDFGTLENYLDGYSIAGDRLAELAVPANILTATD
                     DPVIPVAEFRELKLSASTELTIVPHGGHCGFIRDFSLRSWAEDFILAQMQRRLGI"
     gene            complement(1753..>2159)
                     /locus_tag="VFF05_08390"
     CDS             complement(1753..>2159)
                     /locus_tag="VFF05_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZW51866.1"
                     /translation="ADTWDLLYRVNLRTAVAASQAAIPHLISSSAGRIVNISATGSIK
                     AGAGMGAYAASKSGVARLTEALAEELKDRGITVNAILPSTIDTAANRADMPKADFSRW
                     VTPAQLSDVIVFLLSDRASAVTGALLPVMGRV"
BASE COUNT          325 a          692 c          726 g          416 t
ORIGIN      
        1 actggcgccg gccgctgcgc tatctgtggc gcacgccctg ggtattcgtc cacgtcgtcg
       61 ttgccgtgcc gctcgcgctg gtcgcgttca gtccgatcgg gcgcaccgca catgtgcgcg
      121 gcgaacccct ggatcattgg ctgcaacgct ggtggtcggg tggcatcgtg cgccgttgcg
      181 gattccgcat cgtgcgcgtg ggcgaaccgc aacccggcgc ggtgctgttc gtcgccaacc
      241 acgtgtcgtg gctggacatc gagctgatgc acagccagcg cgtgatgagt ttcgtggcca
      301 aggccgaaat cgcgcgctgg ccgttgatcg gttggctggc gagtcgcgct ggcacgatct
      361 tccatcgccg cggcagcggg cattcgctga acgcagtgat cgagcaggcc atcgaacgcc
      421 tgcgcgccgg cgtggccgtg ggcgtgtttc cggaaggcgg caccgggccg ggcgatcgtc
      481 tgcgcacctt ccatgcgcgc attttccaga tcgccgcgga tgcgcaggtg accgtgcagc
      541 cggtcgcgat cagttacgga cgtgaaggca aaatggattt gcgcgtgccc tttgcggacg
      601 gggagagctt cttcgcgaac gtcgtgcgcc tgctcggcga cgcgggcgtg gaagcgcacg
      661 tccgcttcct cgatccgatt ccattgagcg atgaaggtcg tcgccagacc gccgaatccg
      721 ctcgtgccgc gatcgcgctg gcgttgggtc acgacgacgc atgattgatt cggatgggtt
      781 cgatccgccg cggctgctgc gcaacgcgca tgtgcagtcg gtgctgtcgt cgagtttcct
      841 gcgccgcatc gcgtttcgcc gcaacgacgc cgcactggag aacagtgccg acgaatgcat
      901 tctcgactgc ggcaacggtg tgcgcctgca aggctttcat tccgcgcaga gcgtgcgcgc
      961 cgcgccgcgc gccctggtgg tgttgctgca cggctgggaa ggcagtgcgc agtcgaacta
     1021 catcttgcac accggcgcac gcctgcttgc cgaaagctgc gatgtcttcc gcctgaattt
     1081 ccgcgatcac gggcagacgc accatctcaa ccgcgaattg ttccattcct gccgcatcga
     1141 cgaagtcgtc ggcgcggtgg cggaaatcgc gcggcgcttt ccggcgcgac ctctgatcgt
     1201 cggaggattc tcgctgggcg gcaatttcgc tttgcgcgtg gccttgcgcg ctcccgcagc
     1261 ggggattccg ctggcgtgga cgttcgccgt atgcccggtg atcagcccgc gcgcaggact
     1321 ggctgccatt gaggacgcgc cgtggttcta cgagttctat ttcatgcgca agtggcgcga
     1381 gtcgctgcgc cgcaagcagg ccctgtttcc cgagcgcgaa ctgtttgcac gcggggattt
     1441 gcgcggcggc ctgcgcgacc tcacgcgccg gctggtcgag cagcacacgg atttcggcac
     1501 gctggaaaat tacctggacg gctattccat cgccggagac agactcgccg agcttgccgt
     1561 gccagcgaac atccttaccg ccacggacga tccggtaatt ccggtcgcgg aattccgcga
     1621 actgaagttg tcggcgtcga ccgaactgac catcgttccg cacggcggcc attgcggctt
     1681 catccgcgat ttctcgctgc gcagctgggc ggaggatttc atcctcgcgc agatgcagcg
     1741 gcggctggga atctagactc gtcccatcac cggcagcaac gcgccggtaa cggcactggc
     1801 gcgatccgag agcaggaaca cgatcacgtc ggacagttgc gcgggcgtca cccagcgcga
     1861 aaaatcggcc ttgggcatgt cggcgcggtt cgctgcggtg tcgatggtgc ttggcaggat
     1921 cgcgttgacg gtgatgccgc gatccttcag ttcttcagcc agcgcttcgg tcagccgcgc
     1981 gacgcccgac ttcgatgccg catacgcgcc catgccggcg ccggccttga tcgatccggt
     2041 tgcgctgatg ttgacgatgc gtccggcgct tgaggaaatc agatgcggaa tcgccgcctg
     2101 gcttgccgcg accgcggtgc gcaggttcac gcggtacagc agatcccagg tatcggcgc
//
LOCUS       DASRVO010000677         3261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_280299, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000677 DASRVO010000000
VERSION     DASRVO010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280299"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VFF05_08395"
     CDS             complement(<1..699)
                     /locus_tag="VFF05_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51867.1"
                     /translation="MAKSKWATFPHPDKAYDYAGDKLARAWKALHAGDLEPFPDEKHV
                     AKLLKANPLLAKGAGGKDAAKIAAQLQDAWRAFHRGDFQEAHDIGVALKALGASVAIK
                     AGGIHATYLLKSDKDKLARYAELAELAEAAIAALPDEANSHYRRAFALGRYSQSLSIA
                     QALAQGIAGKVKSSLDTALKLAPKHAEARTALGLYHAEIVGKVGGMLAKFTYGASAGV
                     AEKQLKEALKLTPDS"
     gene            756..2834
                     /gene="ligA"
                     /locus_tag="VFF05_08400"
     CDS             756..2834
                     /gene="ligA"
                     /locus_tag="VFF05_08400"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZW51868.1"
                     /translation="MTSKHSAAERADELRMRLTDANYRYHVLDEPNIPDAEYDALMRE
                     LEGIEAAHPDLRTPDSPTQRIGATPSREFAQVRHAIPMLSLSNAFSDEEVTDFARRIR
                     ERLGRGDPEFSVEPKFDGLAISLRYEDGVFVQGATRGDGETGEDVTANLRTIKAIPLR
                     LRGKEVPRLLEVRGEVYMPRAGFEKYNATARASGGKIKPLVNPRNAAAGSLRQLDSRI
                     TARRPLAFFAYAVGAIEGFDLPATHSATLRRLREFGFPVSDLVGVADGVAGCLKYFNR
                     IGAQRDTLPFDIDGVVYKLDDLAAQRELGFVGRTPRWAIAHKFPAQEQTTTVEAIDIQ
                     IGRTGAATPTARLKPVFVGGVTVTNATLHNADQVARLDVRVGDAVIVRRAGDVIPEVV
                     SVVMDKRPAHTRPWKMPAHCPVCGSAIVRDEGEVVARCSGGLSCAAQIVQSIFHFASR
                     RAMDIDGLGERYIEDLCDLDYVHSVADLYKLSLADLLEMKRRADERDGTTPETVKNGK
                     IATKWAENLIAAIAHSKNTTLDRFLYSLGIPDVGESTAKALARWFGGLDALMQAGQDE
                     LMQVPDVGPIVAAHIATFFAEKHNRDAIAQLRVAGVEWNESEPQREAQGALAGKTVVL
                     TGSLASLTRDEAKEKLEALGAKVAGSVSKKTSFVVAGSEAGSKLDKARELGIEIWDEA
                     RLAEFLRAHE"
     gene            2821..>3261
                     /locus_tag="VFF05_08405"
     CDS             2821..>3261
                     /locus_tag="VFF05_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007966316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZW51869.1"
                     /translation="MHTSESPRTALEGRARLYALIREFFAARGVLEVETPVLSAGANT
                     EPNIASFLTRFSGHVDSGPRGRWLRTSSEFAQKRLLAAGVGDNYELGRVFRDGEAGRR
                     HNPEFTMLEWYRVGWDHRRLIDESIELVQGALALVGKRGEVHKVA"
BASE COUNT          578 a         1005 c         1078 g          600 t
ORIGIN      
        1 cgagtccggt gtgagtttga gcgcttcttt gagctgtttt tcggctacgc cagcgctcgc
       61 gccgtaggtg aacttggcga gcatgccgcc gaccttgccg acgatctcgg cgtgataaag
      121 gccgagggcg gtgcgtgcct ccgcatgttt tggcgcaagt ttcagcgcag tgtccagcga
      181 cgacttcacc ttgccggcga tgccctgtgc cagtgcctgc gcgatggaca ggctttggct
      241 gtagcgacct agcgcgaagg cgcggcggta atggctattg gcctcatcgg gcaaggcggc
      301 gatggccgct tccgcaagct cggcgagttc tgcgtagcgg gccagtttgt ccttgtcgga
      361 cttgagcagg taggtcgcat ggatgccacc ggccttgatt gcgaccgagg cgccgagcgc
      421 tttcaacgcc acgccgatat cgtgggcctc ctggaaatcg ccgcggtgaa acgcgcgcca
      481 ggcgtcctgc aactgggcgg cgatttttgc cgcatccttg ccccctgcgc cttttgcgag
      541 caacggattg gccttgagca gtttcgcgac atgcttttcg tccggaaacg gctccaggtc
      601 gccggcatgc aaggccttcc aggcacgggc cagcttgtcg ccggcgtagt cgtaggcttt
      661 gtccggatgc ggaaatgttg cccacttcga tttggccatg tcggaatcct cgatacgatg
      721 cgcaagcata gcgtggctgc gggtaaactg cgcgcatgac atccaagcat tccgccgccg
      781 aacgcgccga cgaactgcgc atgcgcctga ccgacgccaa ctaccgttac cacgtcctcg
      841 acgagccgaa tatcccggat gccgagtacg acgcgctgat gcgcgaactg gaaggcatcg
      901 aggccgcgca tcccgacctg cgcacgccgg attcaccgac gcagcgcatt ggtgcaacgc
      961 catcgcgcga gttcgcgcag gtgcgccatg caattccgat gctatcgctg tcgaatgcgt
     1021 tcagcgacga agaggtgacg gacttcgcgc gccgcatccg cgaacgactg ggacgcggtg
     1081 atcccgaatt ctccgtggaa ccgaaattcg acggcctcgc gatcagtctg cgctacgagg
     1141 acggggtgtt cgtgcaggga gcgacacgcg gcgacggcga aaccggcgag gacgtcaccg
     1201 cgaatctgcg caccataaaa gccattccgc tgcgcttgcg cggaaaggag gtgccacgct
     1261 tgctcgaagt gcgcggcgag gtctacatgc cgcgtgccgg cttcgagaaa tacaacgcga
     1321 ctgcgcgcgc ttccggcggc aagatcaagc cgttggtcaa tccgcgcaat gccgccgcag
     1381 gttcgttgcg ccagctcgat tcgcgcatca cggcgcgccg tccgctggcg ttcttcgctt
     1441 atgcggtcgg tgcaatcgag ggattcgatt tgccggcgac gcattcggcg acgctgcgcc
     1501 gcttgcgcga attcggcttc ccggtcagcg atctcgtcgg cgttgccgac ggggtggccg
     1561 gttgcctgaa atatttcaac cgcatcggcg cgcagcgcga cacgctgccg ttcgacatcg
     1621 acggcgtggt ctacaaactc gacgatctcg ccgcgcagcg cgaactcggt tttgtaggac
     1681 gcacgccgcg ctgggcgatt gcgcacaagt tcccggcgca ggaacagacg accacggtcg
     1741 aggccatcga catccagatc ggccgcaccg gcgcggccac gccgacggcg cggctcaaac
     1801 cggtgttcgt cggcggcgtc accgtgacca acgcgaccct gcacaacgcc gaccaggtcg
     1861 cgcgcctgga cgtgcgcgtg ggcgatgccg tgatcgtgcg ccgcgccggc gacgtgatcc
     1921 cggaagtcgt ttccgtggtc atggacaagc ggccagcgca cacgcggccg tggaagatgc
     1981 cggcgcattg cccggtatgc ggttccgcca tcgtgcgcga cgaaggcgaa gtcgttgcgc
     2041 gctgctccgg cggcttgagc tgcgcagcgc agattgtgca atccattttt catttcgcct
     2101 cgcgccgcgc gatggacatc gacggcctcg gcgaacgcta catcgaggat ttgtgcgacc
     2161 tcgattacgt ccattccgtg gcggatttgt acaaactgtc cttagccgat ttgctggaaa
     2221 tgaaacgtcg tgccgacgaa cgcgacggca ccacgcccga gacggtgaaa aacggcaaga
     2281 tcgcgacgaa atgggccgag aacctgattg cggcgattgc gcatagcaag aacacgactc
     2341 tggatcggtt tctgtattcg ctgggcattc cggatgtcgg cgaatcgacc gcgaaagcac
     2401 tggcgcgctg gttcggcgga ctcgatgcgc tgatgcaggc gggccaggac gaactgatgc
     2461 aggtcccgga cgtcggtccg atcgtggccg cgcacattgc cacgttcttt gccgagaagc
     2521 acaaccgcga cgcgattgcg cagctgcgcg ttgccggcgt cgagtggaat gaatccgaac
     2581 cgcagcgcga agcgcagggt gcgctcgccg gcaagaccgt tgtgttgacg gggtcgctgg
     2641 cttcgctcac ccgcgacgaa gcgaaagaaa aactcgaagc gctcggtgcg aaagttgcag
     2701 gcagcgtgtc gaagaaaacg agtttcgtcg tcgccggttc agaggccggt tccaagctcg
     2761 acaaggcgcg cgaactcggc atcgagatct gggatgaggc aaggctcgcg gagtttctgc
     2821 gtgcacacga gtgagtcgcc ccgcaccgcg ttggaagggc gcgcgcgtct ctacgccttg
     2881 atccgcgaat tcttcgctgc acgtggcgtc ctggaagtcg aaacgccggt gttgtcggcc
     2941 ggcgcgaaca ccgaaccgaa catcgcaagt ttcttgacgc ggttttccgg gcatgtcgat
     3001 tccgggccgc gcgggcgctg gctgcgcact tcatccgaat tcgcgcaaaa gcgcctgctt
     3061 gctgcgggcg ttggcgacaa ttacgaactc ggccgcgtgt tccgcgatgg cgaggcgggg
     3121 cgccggcaca accccgaatt cacgatgctg gagtggtatc gcgttggttg ggatcatcgg
     3181 cgcctgatcg acgaatccat cgaactcgtg cagggtgcgc tggccttggt cggcaagcgc
     3241 ggggaggtcc acaaggttgc g
//
LOCUS       DASRVO010000678         1408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_253473, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000678 DASRVO010000000
VERSION     DASRVO010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253473"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFF05_08410"
     CDS             complement(<1..769)
                     /locus_tag="VFF05_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZW51870.1"
                     /translation="MLSASPVPAVPSGPGPETYWAMSEPGLLADLLILFALSVAAALA
                     FHRLRLPPIVGFLITGVICGPYGFGLIRNVQEVESLAEIGVILLLFTVGLEFSLQHLA
                     RLRRFLFIGGAAQVGVTIAVTACLARLLAVAWPVAIFLGMLVALSSTAITLRLYAERG
                     ELDTPFGSAALGILIFQDLCVVPMMLLTPALAGAGRSASSLATTLLEAVLFIGATVVA
                     ARLVVPRLLHLVAATRRRDLFLLTIVLFCLGTAWASAR"
     gene            964..>1408
                     /locus_tag="VFF05_08415"
     CDS             964..>1408
                     /locus_tag="VFF05_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HZW51871.1"
                     /translation="MKRHHWVVRVTHWVNVVALTIMVGSGLRIFNAYPAFARKGDTFC
                     CYPFAHKEIPAWLTFGGWLGGARNWHFAAMWVLVLNGLVYLGFIYLHGEWRDLAPRRG
                     DLRDGWEMVKFYAFARRDHPRQGKHNALQKTAYFALPVTGIVSVIT"
BASE COUNT          257 a          401 c          533 g          217 t
ORIGIN      
        1 tccgggccga cgcccacgcc gtgccgaggc agaagaggac gatggtgagc aggaagagat
       61 cgcggcggcg cgtggcggct acgagatgca ggaggcgcgg caccacgaga cgcgcggcca
      121 cgaccgttgc gccgatgaag agaacggcct ccaacaaggt cgtggcgagg gagctcgcgc
      181 tgcggccggc gccggcgagc gccggcgtga ggagcatcat cggtacaacg cacagatcct
      241 gaaagatcag gatgccgagt gccgcgctgc cgaacggcgt gtcgagctca ccgcgttcgg
      301 cgtaaagccg cagcgtgatc gcggtgctgc tgagcgccac gagcatgccg aggaaaatgg
      361 cgaccggcca cgcgacggcg agcagtcggg cgagacacgc cgtgacggcg atcgtcactc
      421 cgacctgcgc ggcgccgccg atgaacagga agcgtcggag ccgagcgagg tgctggagcg
      481 agaattcgag tccgactgtg aacagaagca ggatcacgcc gatttcggcg agcgactcca
      541 cttcctgcac gtttcggatg agaccgaatc cgtagggtcc gcaaatgacg ccggtgatga
      601 ggaacccgac gatcggcgga agtctgagcc gatggaacgc gagcgccgcc gcgacgctca
      661 gtgcgaagag gatcaggagg tcggcgagga gtcccggctc ggacatggcc caatatgtct
      721 ccggaccggg cccgctggga acggcgggca ctggggaggc tgacagcatc tcgtggggag
      781 cggtgtatga atcggcgtgg acaccgctgc agaggtggcg ggcgacatcg taggatcgcg
      841 cggccagtgc gcgccatgca cggcgcggcg gggagaggcc gcgcgacgcg gctttcgtgt
      901 tgcgtcgcgt tcgcgtaggt tggctgctgc cgagcgattc gttgggattg tgcgggagga
      961 acggtgaagc gacatcactg ggtcgtgcgc gtcacgcatt gggtgaacgt ggtggcactc
     1021 accatcatgg tgggaagcgg cctgcgcatc ttcaacgcgt atccggcatt cgcgcggaag
     1081 ggggacacgt tttgctgtta tccgttcgcg cacaaggaaa tcccggcgtg gctgacgttc
     1141 ggtggttggc tgggcggcgc gcgcaactgg cacttcgcgg cgatgtgggt gctggtgctg
     1201 aatgggctcg tgtacctcgg tttcatctat ctgcacggcg agtggcgcga tctcgcgccg
     1261 cgccgcggcg atctgcgcga cggatgggag atggtgaagt tctacgcatt cgcacggcgc
     1321 gatcatccgc ggcagggtaa gcacaacgcg ctccagaaga cggcgtactt cgcactgccg
     1381 gtgaccggta tcgtgtcggt gatcacgg
//
LOCUS       DASRVO010000679         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_174484, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000679 DASRVO010000000
VERSION     DASRVO010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174484"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFF05_08420"
     CDS             <1..197
                     /locus_tag="VFF05_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008433789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HZW51872.1"
                     /translation="ARTVCRRAERGTVTLARSESVRPEAIRYLNRLSDLLFVLARVLA
                     RASGHGEVLWQHERRKRPKS"
     gene            198..1112
                     /locus_tag="VFF05_08425"
     CDS             198..1112
                     /locus_tag="VFF05_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HZW51873.1"
                     /translation="MLLYTHPACLAHDPGIGHPESPARLRTVLDALDDPSFAGLVRRE
                     APRATRAQLARVHAESLIDAVFAAAPSIGHAQLDADTAMSPDSLEAALRAAGAVCAAV
                     DALIDGEDTRAFCAVRPPGHHATRDTAMGFCLFNNVAAGATQALTRGLVRAAIVDFDV
                     HHGNGTQDIFWNEPRVLYASTHQSPLYPGTGARTETGVGNIVNAPLPPGAGSAEFRSA
                     FAQIVLPRLHDFRPQLVLISAGFDAHRLDPLASLEFSADDYAWVTRELAQIARTHAQD
                     RIVSSLEGGYSLTALRESTAAHVAALME"
     gene            complement(1114..1509)
                     /gene="msrB"
                     /locus_tag="VFF05_08430"
     CDS             complement(1114..1509)
                     /gene="msrB"
                     /locus_tag="VFF05_08430"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZW51874.1"
                     /translation="MNSKIEKTDAQWRAELDPAKYAVCRCSATEPPFTGKYWDHHETG
                     TYRCAACGADLFASTAKFDSGSGWPSYFQPLTKAAVSEHADESHGMRRVEIRCARCES
                     HLGHVFPDGPRPTGLRYCINSVALDFKPE"
     gene            1629..>2706
                     /locus_tag="VFF05_08435"
     CDS             1629..>2706
                     /locus_tag="VFF05_08435"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HZW51875.1"
                     /translation="MSGANRSRLPSFRLLATALALILVQRAIAQDMAPACPVGVLICP
                     RPARTNTFALCKRNALPDFYTPGLPTQGDRSQVAADVSARKVESTDSTHYRLDGDVRM
                     QRLDQLLRSDFLTWDTDTTAYTAGGHVVMQDRSMVMSADSASGVESAHSTKLANVRYQ
                     LLQERGNGTAASADMSDAEHGKLSDGTYTTCDPDDVRWHLHANELDMDKLANVGRAHG
                     VSLYYGDVPFFWFPYLRFPLSNERQSGFLAPNIGYSSHRGLVLGAPYYLNLAPNYDAT
                     LEPRVSTERGAMLAGQFRFLDAADKAQIDFNFVPHDNEAADERAQYAATPPVFQGPAS
                     PVDIPDRRYAFQIKDWSTFSENWGA"
BASE COUNT          475 a          938 c          829 g          464 t
ORIGIN      
        1 tggcacgcac cgtgtgccgg cgcgcggagc gcggaaccgt cacgttggcg cgttccgaat
       61 ccgtgcgccc cgaagcaatc cgttatttga accggctgtc cgacctgctg ttcgttctcg
      121 cgcgcgtgct ggcgcgcgcg tcgggacacg gcgaagtgct gtggcaacac gaacgcagaa
      181 aacgaccgaa atcctgagtg ctcctttaca cccaccccgc ctgcctcgcc cacgatcccg
      241 gcataggtca tccggaatcc cccgcgcggc tgcgcaccgt actcgacgca ttggatgacc
      301 caagtttcgc cggactcgtg cgccgcgaag ctccgcgcgc cacgcgcgcg caacttgccc
      361 gcgtccacgc cgaatcgctg atcgacgcgg tatttgccgc ggcgccgagc ataggtcacg
      421 cgcaactcga tgcggacacg gccatgtcgc ctgattctct ggaggccgcc ttgcgcgcgg
      481 ccggcgcagt gtgcgccgcg gtggacgcgt tgatcgatgg cgaggatacg cgcgcgttct
      541 gtgccgtgcg cccgcccggc catcacgcca cccgcgacac cgcgatggga ttttgcctgt
      601 tcaacaacgt cgcggccggt gccacacaag cgctgacgcg cgggctcgta cgcgcggcca
      661 tcgtcgattt cgacgtgcac cacggcaacg gcacgcagga catcttctgg aacgaaccgc
      721 gcgtgttgta cgcatccacg caccaatcgc cgctgtatcc cggcaccggc gcgcgcacgg
      781 aaaccggagt gggcaacatc gtcaacgcac cgttgcctcc gggtgccggc tcggcggaat
      841 tccgctcagc gttcgcccag atcgtgttac cgcgcctgca cgatttccgt ccgcaactgg
      901 tgctgatttc ggccggcttc gacgcgcacc ggctcgaccc gctggccagc cttgaattca
      961 gtgcggacga ttacgcatgg gtgacgcgcg aactcgcgca aatcgcccgc acgcacgcgc
     1021 aggaccgcat cgtttccagc ctcgaaggcg gctacagcct gaccgcgctg cgcgaatcca
     1081 cggccgcgca cgtcgcagcg ctgatggagt gaatcactcc ggtttgaagt ccaaagccac
     1141 cgaattgatg cagtagcgca agccggttgg acgcggacca tcgggaaaaa cgtggccaag
     1201 gtgcgactcg cagcgcgcgc agcgtatctc gacgcggcgc atgccgtggg attcgtctgc
     1261 gtgctcgctg accgctgctt tcgtcagtgg ttggaagtag ctgggccagc cgctgccgga
     1321 atcgaatttc gcggtcgagg cgaacaagtc cgcgccgcag gccgcgcagc gatacgtgcc
     1381 cgtttcatgg tgatcccagt acttgcccgt gaatggtggc tcggtcgccg aacaacggca
     1441 cacggcatat ttcgccggat ccagttccgc acgccattgc gcgtccgttt tttcgatttt
     1501 cgagttcatt gcagactcct ttctaccctc atcagaccgg cgcaggtgaa caaaaattgc
     1561 accggcgcgt tgcccggaaa cggcgaatca ggcaagcttc gcgcttgcgt atcgcaatcg
     1621 gatcatccgt gtctggcgcc aaccgttcaa gattgccgag tttccgcctg ctggccaccg
     1681 cactggcgct gatcctcgtc cagcgtgcga tcgcacagga catggctccc gcctgcccgg
     1741 tcggcgtact gatctgcccc aggcccgcgc gcacgaacac gttcgcgctg tgcaagcgca
     1801 acgcgttgcc ggatttctac acgcccggcc tgccaacgca gggcgaccgc agccaggtcg
     1861 cggccgacgt cagcgcgcgc aaggtcgaat ccacggacag cacgcactac cggctcgacg
     1921 gcgacgtgcg catgcaacgc ctggatcaac tgctgcgttc ggatttcctc acctgggaca
     1981 ccgacaccac ggcctacacc gccggcggcc acgtcgtgat gcaggatcgc agcatggtga
     2041 tgtcggccga ttcagccagc ggcgtggaat cggcgcattc gaccaaactt gccaacgtgc
     2101 gctaccagtt gctgcaggag cgcggcaacg gcaccgctgc cagcgccgac atgagcgatg
     2161 ccgagcacgg caagctgtcc gatggcacgt acaccacgtg cgatccggac gatgtgcgct
     2221 ggcatctgca cgccaacgag ctggacatgg acaagctcgc caacgtcggc cgtgcgcatg
     2281 gcgtgagcct gtactacggt gacgtgccgt tcttctggtt tccgtacctg agattcccgc
     2341 tcagcaacga gcgccaatcc ggattcctcg cgccgaatat cgggtattcc agccaccgcg
     2401 gcctggtgct gggcgcgccg tattatctga atctcgcgcc caactacgac gccaccctcg
     2461 aaccgcgcgt gtcgaccgaa cgcggcgcca tgctcgcggg ccagttccgg tttctcgacg
     2521 ccgccgacaa ggcgcagatc gatttcaatt tcgtcccgca cgacaacgag gctgcggacg
     2581 agcgcgcgca gtacgccgcc acgccgccgg tgttccaggg accggcctcg ccggtggaca
     2641 ttcccgaccg tcgctacgcg ttccagatca aggactggag cacgttcagc gaaaactggg
     2701 gcgcgg
//
LOCUS       DASRVO010000680         1580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_253533, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000680 DASRVO010000000
VERSION     DASRVO010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253533"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..576)
                     /gene="tsaE"
                     /locus_tag="VFF05_08440"
     CDS             complement(133..576)
                     /gene="tsaE"
                     /locus_tag="VFF05_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZW51876.1"
                     /translation="MRAMQTQREFLTHSSDETIARGREIAAGLKPPILILLSGELGAG
                     KTTLTKGIVTGLGAAREEDVTSPTFTLVHKYQGRTRVYHVDLYRISGFHDLETLGLED
                     LFSEDAVVIVEWPDRLTLRTDWPILRIELEHVSDEARRIRLRSQL"
     gene            complement(576..>1580)
                     /locus_tag="VFF05_08445"
     CDS             complement(576..>1580)
                     /locus_tag="VFF05_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZW51877.1"
                     /translation="LRDASRIAGQLAVRDIGSPPELIEEIGQGNLRWSEPNEFCAKFA
                     ARRSPEGHKGDYGHALIVAGSVGKTGAAVLASWAALRVGAGLVTVATPQPALPIIAAH
                     TPEVMTAPLAATTAGTVSLRSLDGGYFDSLVEGKRALGIGPGLTTNDETVQFVRKVVA
                     QRAAPIVLDADGLNAFAGRAAELKSPSGLLGITPHPGEMARLLACGIQDVQSNRADVA
                     LKAAAGWNCVVVLKGHQTAIASPTGEAFINSTGNPGMGTGGTGDVLTGMLAGLVAQFG
                     HELNYSGYAQLLAFSVYLHGLAGDIAYADHGEAPLIASDLIHALPRAYQQFYADCDRG
                     "
BASE COUNT          271 a          437 c          605 g          267 t
ORIGIN      
        1 gcagatccct cacccccatt cccgcacaag gcgcgagact gggttcggga tgacacttgg
       61 gggcattccc ccaacggaac gagggcacgg agacgtagag gtcaggaacg gacgcgggga
      121 gcagacgatt gtctacaact ggctgcggag gcggatgcgg cgggcttcgt cggaaacgtg
      181 ttcgagttcg atgcgcagga tgggccagtc ggtgcgcagt gtgaggcggt cgggccactc
      241 gacgatcacc accgcatctt cggagaagag gtcttcgagg ccgagggttt cgagatcgtg
      301 gaagccggag atgcgataga ggtcgacgtg atagacgcgc gtgcgaccct gatatttgtg
      361 gacgagcgtg aacgtggggc tggtgacatc ctcttcgcgg gcggcgccca ggccggtgac
      421 gatgcctttg gtcagcgtgg tcttccctgc cccgagttcg ccggaaagca ggatcaggat
      481 gggcggcttg aggccggcgg cgatttcgcg gccgcgcgcg atggtttcgt cggaggagtg
      541 ggtgaggaat tcgcgctgag tttgcattgc gcgcatcagc cgcgatcgca atcagcatag
      601 aactgctggt aggcgcgagg aagggcgtgg atcaaatcgg aggcgatgag gggcgcctcg
      661 ccgtggtcgg cgtaagcgat gtcgccagcg aggccgtgga ggtagacgct gaaggcaaga
      721 agctgcgcgt agccggagta gttgagctcg tggccgaatt gcgcgacgag gcctgcgagc
      781 atgccggtga gcacgtcgcc ggtgccgccc gtgcccatgc cgggattgcc ggtggaattg
      841 atgaaggctt cgccggtggg cgaggcgatc gccgtttgat ggcctttgag gacgacgaca
      901 cagttccagc cggcggcggc tttgagcgcg acatcggcgc gattggactg cacgtcctgg
      961 atgccgcagg cgagcaggcg cgccatttct ccgggatgag gcgtgatgcc gagaaggccg
     1021 ctcgggcttt tcagttcggc ggcgcggccg gcgaaggcat tcaggccgtc ggcatcgaga
     1081 acgatcggcg cggcgcgctg cgcgacgact ttgcgcacga attgcacggt ttcgtcattc
     1141 gtggtcaggc cgggtccgat gccgagcgcg cgtttgcctt cgacgagcga atcgaaatag
     1201 ccgccgtcga ggctgcgaag cgagacggtg ccggcggtgg tggcggcgag gggcgcagtc
     1261 atcacttcgg gagtatgcgc ggcgatgatg ggcaacgcag gctgcggcgt ggcgacggtg
     1321 acgagtccgg cgccgacgcg cagcgcggcc caagaagcca gaacggcggc gccggtcttg
     1381 ccgaccgagc cggcgacgat gagggcgtgg ccataatcgc ccttgtggcc ttcgggcgaa
     1441 cgtcgcgccg caaatttcgc gcagaattcg ttcggctcgg accagcgcag attgccttgg
     1501 ccgatttcct cgatcaattc gggcggcgag ccgatatcgc ggacggccag ctggccggcg
     1561 atgcggcttg cgtcgcgaag
//
LOCUS       DASRVO010000681         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_46696, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000681 DASRVO010000000
VERSION     DASRVO010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46696"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VFF05_08450"
     CDS             complement(<1..365)
                     /locus_tag="VFF05_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HZW51878.1"
                     /translation="MIRLSVWPLTAAVVLLAGCAVPPRPSHPDLSGAAPLAGVPSDAN
                     AAWPESSWWHRYGDEQLNDLEARALSGSPTLAVARARFTQATSSVDVAKANGGASVSG
                     SAQVQRERLSEHGLIPSEFL"
     gene            complement(362..886)
                     /locus_tag="VFF05_08455"
     CDS             complement(362..886)
                     /locus_tag="VFF05_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZW51879.1"
                     /translation="MAEFPACPSFAVIESSIGRVAERLRGAPVRDIVLLRLIKHLSAQ
                     FGVHLNRMVRPTGLNEVGFRTLMMLYANTAAGVFASQLSEAAGETRANMTRICDELAR
                     KGLLRRRAGTADRRRVALEITRKGESLIERLLPKMWSELDLSMRALSAREKLDLERLL
                     KKLAAALDAKGQTA"
     gene            complement(971..>1870)
                     /gene="kynU"
                     /locus_tag="VFF05_08460"
     CDS             complement(971..>1870)
                     /gene="kynU"
                     /locus_tag="VFF05_08460"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HZW51880.1"
                     /translation="IEKNPFPSDRYAVESQIRFHGFDPATSLIELESDEANGSISAAA
                     IERAIGEHGSRIALVLLPGVQYRTGQVFDLKRIAALAHARGCMVGYDLAHSAGNVPVD
                     LHDSGCDFAVWCSYKYLNSGPGAVAGCFVHERHARTDRPRFAGWWGHDQSTRFKMGPD
                     FSPTPGADGWQLSNPPILALAPLRVSLEIFHRAGLARLREKSVALTGYLDTLIRKQLA
                     GIIDIATPRDPERRGCQLSLRVVNGHNAGRALFDYLGTHGIVGDWREPDTIRIAPTPL
                     YNRYLDCLQFVRAAQNWANFART"
BASE COUNT          316 a          575 c          665 g          314 t
ORIGIN      
        1 aggaactcgc tgggaatgag tccatgttcg ctcaggcgtt cacgttgcac ctgcgcgctg
       61 ccgctgacgc tggcgccgcc gttggccttg gcgacatcca cgctggacgt ggcctgggtg
      121 aagcgggcgc gcgcgacggc cagcgtgggc gagccgctca gcgcacgcgc ttccaggtca
      181 ttcaattgct cgtcgccgta gcggtgccac catgacgatt ccggccaagc agcgttcgca
      241 tcggacggca caccggcgag cggtgcagcg ccggataggt cagggtgcga cgggcgcggc
      301 gggaccgcgc aaccggcaag caacacgacc gctgcggtca gcggccaaac ggaaagacga
      361 atcatgcggt ttgtcccttg gcatccagcg cggcagcgag ttttttcagc aggcgttcca
      421 gatcgagctt ttccctggca ctcagcgcac gcatgctaag gtccagttcc gaccacatct
      481 tcggcagcag gcgctcgatc agggactcgc ccttgcgcgt gatttccagc gcgacgcggc
      541 ggcgatccgc ggtgccggcg cggcgccgca gcaggccctt gcgcgcgagt tcgtcgcaga
      601 tgcgcgtcat gttggcgcgg gtttcgccgg ctgcttcgct gagttgcgag gcgaacaccc
      661 cggccgccgt gttcgcgtac agcatcatca gcgtgcggaa gccgacctcg ttgaggccgg
      721 tcgggcgcac catgcggttg aggtgcacgc cgaattgcgc cgacaggtgc ttgatcaggc
      781 gcagcaacac gatgtcgcgt accggtgcgc cgcgcaggcg ctcggcgacg cggccgatgc
      841 tggattcgat gacggcaaaa ctggggcagg cggggaactc agccatggcg atagttcagg
      901 tttcaactat cgcaatacta acaagtacat taattgagcg caagcatggc gtgatgggtc
      961 cgatgcgtgc tcaggtcctg gcgaagttgg cccagttttg cgcggcccgc acgaactgca
     1021 ggcagtcgag atagcggttg tacagcggcg ttggcgcgat gcggatcgtg tcgggttcgc
     1081 gccagtcgcc gacgatgccg tgcgtgccga ggtaatcgaa cagggcgcga cccgcattgt
     1141 gtccgttcac gacgcgcagc gagagctggc agccgcggcg ctcgggatcg cgcggggtgg
     1201 cgatgtcgat gatgccggcg agctgcttgc ggatcagcgt atcgagatag ccggtcaggg
     1261 cgacggattt ctcgcgcagc cgcgcgagtc cggcgcggtg gaagatttcc agcgacaccc
     1321 gcagcggcgc gagcgcgagg attggcggat tcgacaactg ccagccatcg gcgccgggtg
     1381 tcggcgaaaa atccgggccc atcttgaagc gcgtggactg gtcgtgcccc caccagccgg
     1441 cgaagcgcgg ccggtcggtg cgcgcgtggc gctcgtgcac aaaacagccg gcgaccgcgc
     1501 cgggtccgga gttgaggtac ttgtagctgc accacacggc gaagtcgcag ccgctgtcgt
     1561 gcaggtcgac cggcacgttg ccggccgaat gcgcgaggtc gtagccgacc atgcagccgc
     1621 gggcatgggc aagagcggcg atgcgtttga ggtcgaacac ctggccggtg cggtactgca
     1681 cgcccggcag cagcaccagc gcgatgcgcg aaccgtgttc gccgatggcg cgttcgatgg
     1741 cggcggcgga gatcgatcca ttcgcctcgt cgctttcgag ttcgatcagc gatgtcgccg
     1801 gatcgaagcc gtggaaacgg atctgcgact cgaccgcgta gcgatccgac ggaaacggat
     1861 ttttttcgat
//
LOCUS       DASRVO010000682         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_356773, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000682 DASRVO010000000
VERSION     DASRVO010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356773"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VFF05_08465"
     CDS             complement(<1..328)
                     /locus_tag="VFF05_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZW51881.1"
                     /translation="MATKSNTRTKTSSIASRKRHGAARTSPDAVVRHYYKGNRLKQLR
                     AFCYAVKFGTVARAAEALFLSASSASLQLSALEKELGTRLLERTRPRLSLTREGQMLY
                     DLARPLI"
     gene            456..1757
                     /gene="aceA"
                     /locus_tag="VFF05_08470"
     CDS             456..1757
                     /gene="aceA"
                     /locus_tag="VFF05_08470"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HZW51882.1"
                     /translation="MKTTLPTAEQIKIDWQNNPRWNGVKRPYTAEDVVRLRGTVHVEH
                     SLARLGAEKLWKSLHNEDFVNALGALTGNQAMQQVKAGLKAIYLSGWQVAADANLAGQ
                     MYPDQSLYPADSVPAVVRRINNALLRADQLHHAEGNDAFDFLQPIVADAEAGFGGVLN
                     AFELMKAMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVDKLAAARLAADVMGV
                     PTLLVARTDAEAADLITSDIDANDKPFTTGERTVEGFYKTRNGLDQAISRGVAYAPYA
                     DLVWCETGKPDLKFARAFAEGVHAKYPGKLLAYNCSPSFNWKKNLDDATIAKFQRELA
                     AMGYKFQFITLAGFHALNYSMFNLAHGYARRQMSAFVELQEAEFAAAERGFTAVKHQR
                     EVGTGYFDTVTQTIQGSGASTVALKGSTEEAQFHGQEKAAA"
     gene            1869..2906
                     /locus_tag="VFF05_08475"
     CDS             1869..2906
                     /locus_tag="VFF05_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HZW51883.1"
                     /translation="MSTVPTDAAAEVVAANLAPRAQAPHLPRQLSDAEFVMLRGAGPA
                     RRCAAGEVLFRKGEIGQNMFVIESGQVRLDFGDGLPSKVLGPREYFGELALFIGNHAR
                     VAGAVAHSACGLRMIDYPAFDFLLENEPALLAQFMRRSFAYLVASEQQLIASLKRRNE
                     DLLVTLDSLRQTQSQLSTANRLVRTDELTGLVNRRGLYEHLEHLVRFRVAGTHLALLL
                     IDLDRFKQINDEFGHLAGDQVLCAIAAEVSRVASACDLPCRIGGDEFALLAQLNEATE
                     LEALAARIVSGIRALRFVEPLENVSASISIGAGMCDDNADWSVWYSQADRALYQAKGQ
                     GGNSWSVAASD"
     gene            2988..>3431
                     /locus_tag="VFF05_08480"
     CDS             2988..>3431
                     /locus_tag="VFF05_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptide modification system cyclase"
                     /protein_id="HZW51884.1"
                     /translation="MSAVPEDVQTQIPASVTPAPLLRAVLVCDLTDSAQLTQRLGDVE
                     AAELAQRLDRIARDLVYRHHGREIDKSDGFLLLFERPVYAVAFALDYQRRLRELATAE
                     SLPLSAHIGIHVGDVVLWENTPADVARGAKPTEVEGLAKPVAARLM"
BASE COUNT          629 a         1122 c         1092 g          588 t
ORIGIN      
        1 cgatcagcgg gcgcgcgagg tcgtacagca tctggccttc gcgcgtgagc gacaggcgcg
       61 ggcgcgtgcg ttcgagcagg cgcgtgccca gctccttttc cagggcagat agctgcaagc
      121 tggccgacga tgccgacagg aacagcgctt cggccgcgcg cgccaccgtg ccgaacttca
      181 cggcgtagca gaacgcacgc agttgcttga ggcggttgcc cttgtagtag tggcggacga
      241 cggcgtcggg ggatgtcctt gccgccccgt gccgctttcg cgatgcgatc gagctggttt
      301 tcgtacgagt gttcgatttc gtggccattt cattcaatag gatacatcat atttgacaaa
      361 gtcaaacgta cagattgaaa cattcagatt gtcaaatcgt gttttgcaag cctagtctgc
      421 atgcgttgca ttcccaacca ctttcgagga tcacgatgaa gaccacgctg cccaccgccg
      481 aacagatcaa gatcgactgg cagaacaacc cgcgctggaa cggtgtgaag cgaccctaca
      541 cggccgaaga cgtggtgcgc ctgcgcggca ccgtccatgt cgagcattcg ctggcgcggc
      601 tgggtgccga aaaattgtgg aagtcgctgc acaacgagga tttcgtcaac gcgctcggtg
      661 cgctcaccgg caaccaggcc atgcagcagg tcaaggccgg attgaaggca atttatcttt
      721 ccggttggca agtcgcggcg gacgcgaacc tcgccggcca gatgtatccg gatcaatccc
      781 tgtacccggc cgattcggtg ccggcggtcg tgcgccgtat caacaacgcg ctgttgcgcg
      841 cggaccagct gcatcatgct gaagggaacg atgcgttcga cttcctgcaa cccatcgtcg
      901 ccgatgccga ggccggcttc ggcggcgtgc tcaatgcatt cgagctgatg aaggcgatga
      961 tcgaggcggg cgccgcgggc gtgcatttcg aagaccagct cgccagcgtg aagaaatgcg
     1021 ggcacatggg cggcaaggtg ctggtgccca cgcgcgaagc ggtggacaag ctcgccgccg
     1081 cgcgtctcgc ggcggatgtc atgggcgtgc cgaccctgct ggtcgcgcgc accgacgcgg
     1141 aagccgccga cctgattacc tccgacatcg acgccaacga caagccattc acgaccggcg
     1201 agcgcaccgt cgaaggcttc tacaagacgc gcaatggatt ggaccaggcg atttcgcgcg
     1261 gcgtcgccta tgcgccgtac gcggacctcg tgtggtgcga gaccggcaag ccggacctca
     1321 aattcgcgcg cgccttcgcc gaaggcgtgc acgcgaaata tccgggcaag ctgctcgcct
     1381 acaactgctc gccaagcttc aactggaaga agaacctgga cgacgcgacc atcgcgaaat
     1441 tccagcgcga actcgcggcg atgggctaca agttccagtt catcaccctc gccggcttcc
     1501 atgcgctcaa ctattcgatg ttcaacctcg cccacggcta cgcgcgccgc cagatgagcg
     1561 ccttcgtcga attgcaggaa gccgaattcg ccgccgccga gcgcggcttc accgcggtca
     1621 agcaccagcg cgaagtcggt accggctact tcgacacggt cacgcagacg atccagggca
     1681 gcggtgccag cacggtcgcg ctgaaaggct cgaccgaaga agcgcagttc cacgggcagg
     1741 agaaagcggc ggcgtaagcg ccgcgacacg tccgttcatc gtccaggcat acccggccat
     1801 ccgtgccggg tgtgcatttg cgcccgctgc gctaccatgc gccggttcag gacgagggga
     1861 atacggacgt gtccaccgtg ccaaccgatg ccgccgcgga agtcgtggcg gcgaacctgg
     1921 cgccgcgcgc gcaggcgccg cacctgccgc ggcagttgtc cgatgccgaa ttcgtcatgc
     1981 tgcgcggcgc cggccccgcg cgccgctgtg ccgccggcga ggttttgttc cgcaagggcg
     2041 aaatcggcca gaacatgttc gtgatagaaa gcgggcaggt gcgcctggat ttcggcgacg
     2101 gcctgccgag caaggtactc ggtccgcgcg aatatttcgg cgaacttgcg ctgttcatcg
     2161 gcaatcatgc gcgcgtggcc ggcgcggtcg cgcacagcgc atgcggcctg cgcatgatcg
     2221 actaccccgc cttcgacttt ctgctcgaaa acgaaccggc cctgctcgcg cagttcatgc
     2281 gccgctcgtt cgcctacctc gtcgccagcg agcagcagtt gatcgcgagc ctgaaacggc
     2341 gcaacgagga tctgctggtc acactggatt cgctgcgcca gacgcagagc cagctttcca
     2401 ccgcgaatcg cctggtgcgt accgacgaac tcacgggact cgtcaatcgc cgtggccttt
     2461 acgaacacct ggaacacctc gtccggttcc gcgttgccgg cacccatctc gcgctgctgc
     2521 tgatcgatct cgaccgcttc aagcagatca acgacgaatt cggccatctc gccggcgatc
     2581 aggtgctgtg cgcgatcgct gccgaagtca gccgcgtcgc ctcggcctgc gatctgccct
     2641 gtcgcatcgg cggcgacgaa ttcgccctgc tcgcccagct caacgaagcg acggaactcg
     2701 aagcgctggc cgcacgcatc gtctcgggta tccgcgcgct gcgcttcgtc gaaccgctgg
     2761 aaaacgtgag cgcgtcgatc agcatcggcg ccggaatgtg cgacgacaac gccgactggt
     2821 cggtgtggta cagccaggcc gatcgggcgc tgtaccaggc caaggggcag ggtggcaatt
     2881 catggagcgt ggctgcgtcc gattagccca caagttggca tagccacgcc atttggcgca
     2941 caatctctcg actgcgacgg cggtcaggcc gtgcgcggcg ggggtgaatg agtgcagtgc
     3001 ccgaggatgt gcaaacgcag attccggcgt cggtgacacc ggcgccgctg ctgcgcgccg
     3061 tgctcgtctg cgatctgacc gacagcgcgc aactgaccca gcgcctgggc gacgtcgagg
     3121 ctgccgaact tgcgcaacgc ctcgaccgca tcgcccgcga tctcgtctat cggcatcacg
     3181 gccgggaaat cgacaagtcc gacggcttcc tgctgttgtt cgagcgtccg gtgtacgcgg
     3241 tcgccttcgc gcttgactac cagcgccgcc tgcgcgagct cgccactgcc gaatcgctgc
     3301 cgctgtcggc gcacatcggc atccacgtcg gcgacgtggt gttgtgggaa aacacaccgg
     3361 ccgatgtcgc gcgcggcgcc aagcccaccg aagtcgaagg cctggccaaa cccgtggcgg
     3421 cgcggttgat g
//
LOCUS       DASRVO010000683         1177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_149019, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000683 DASRVO010000000
VERSION     DASRVO010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149019"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VFF05_08485"
     CDS             complement(<1..1167)
                     /locus_tag="VFF05_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HZW51885.1"
                     /translation="MVAVVTGKDAPNTYGILPIGHDEHALCVDKARYVGDNVACVAAI
                     DEATAENALELIDVDYELLPAWFDPEESMKAESEFIHENRPHNIEKDYHHVFGDPDRG
                     FAESDYVAEARYIANEVTHAAMEPHSTLASFEFDSQTGHPGRLTVWSSTQVPYYLQHK
                     LSLVLDMPMSQIRVIKPLVGGGFGGKSEVIPLEIIAAVTARAAHAPVKITYTREEVFW
                     AHRGRPRTIVDLKTGVKKDGRIMSVSARVVQDGGGYCSYGPVTILYSGALLGALYDIP
                     NVRYDGFRVLTNKPACGAMRGHGTVNVRFAFESQMDEIAAALKLDPAEVRRINLLKPP
                     CITVNGLRVLSYGLPECIEQVVNRSQWRERRGKRAGQVRPTLKRGLGLACSHYVS"
BASE COUNT          199 a          368 c          398 g          212 t
ORIGIN      
        1 actcacgtaa tgcgaacagg cgaggcccag gccgcgcttc agcgtcggcc gcacctggcc
       61 cgcgcgtttg ccgcgccgtt cgcgccactg cgaacggttc accacctgct caatgcattc
      121 gggcaggccg tagctcagca cgcgcaagcc gttcacggtg atgcacggcg gtttgagcag
      181 attgatgcgg cggacttccg cgggatcgag cttaagggcg gcggcgattt cgtccatctg
      241 cgattcgaag gcgaagcgca cgttcaccgt gccgtggccg cgcatggcgc cgcaggcggg
      301 cttgttggtc agcacgcgga agccgtcgta gcgcacgttg ggaatgtcgt agagcgcacc
      361 cagcagcgcg ccggaataaa gaatcgtgac cggcccgtag gagcagtagc cgccgccgtc
      421 ctgcaccaca cgcgcggaca cggacatgat gcggccgtcc ttcttgacgc cggttttcag
      481 gtccacgatg gtgcgcgggc gcccgcgatg cgcccagaac acttcctcgc gggtgtaggt
      541 gatcttcacc ggcgcatgcg cggcgcgcgc ggtcacggcg gcgatgattt ccagcgggat
      601 gacttcgctc ttgccgccga agccgccgcc caccagcggc ttgatgacgc ggatctgcga
      661 catcggcatg tcgagcacca gcgacagttt gtgttgcagg tagtaaggaa cctgggtgga
      721 cgaccacacg gtcaagcgcc cgggatggcc ggtctgcgaa tcgaactcga acgacgccag
      781 ggtggagtgc ggttccatgg cggcgtgggt gacttcgttg gcgatgtagc gcgcctcggc
      841 gacgtagtcg ctttcggcaa aaccgcggtc gggatcgccg aagacgtggt gatagtcctt
      901 ctcgatgttg tgcggccggt tctcgtggat gaattccgac tcggccttca tggactcttc
      961 gggatcgaac cacgccggca gcagctcgta atccacatca atcagttcga gagcgttctc
     1021 cgcggtagcc tcatcaatgg cggcgacgca ggccacgtta tcgcccacgt agcgcgcctt
     1081 gtccacgcac agcgcgtgct cgtcgtggcc gatgggaagg atgccgtagg tgttgggggc
     1141 gtccttgccg gtaacgacgg cgaccacgcc ctccagg
//
LOCUS       DASRVO010000684         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_97402, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000684 DASRVO010000000
VERSION     DASRVO010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97402"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFF05_08490"
     CDS             <1..203
                     /locus_tag="VFF05_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51886.1"
                     /translation="PFGPVMRMAWIPRPAVDAAPRIAMEPGPLWLIRGKEAGKNCRFG
                     SFLPVARARDRPSPAQKLLLQQ"
     gene            300..>1007
                     /locus_tag="VFF05_08495"
     CDS             300..>1007
                     /locus_tag="VFF05_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HZW51887.1"
                     /translation="MLALLMIAFAVPNAGATSRIKDLANIEGVRQNQLIGYGLVVGLN
                     GTGDTLNNIPFTKQSLTAMLERLGVNIRGQTLRTGNVAAVMVTANLPAFGTQGTRIDV
                     TVSSLGDAKSLQGGMLLVTPLLGADGNVYAVGQGSVAIGGFQAEGEAAKIVRGVPTVG
                     RIANGALIEREIEFTLNKLSQLRMALRNPDFTTAKRVAAAINDFIGVPTAEPLDPATV
                     GITIPQQYRGNVISLLTE"
BASE COUNT          189 a          348 c          302 g          168 t
ORIGIN      
        1 ctccgttcgg cccggtaatg cgcatggcgt ggattccccg tccggctgtc gacgccgctc
       61 ctcgaatagc catggaacct gggccgttat ggttaatccg cggtaaagag gccggcaaaa
      121 actgccggtt cggatcattc ctgccggtcg cgcgggcccg cgaccgtccc tcaccggctc
      181 aaaaactatt gctccagcaa tgacttaacc gcgcgcggcg aagtggcatg cgcttcgcat
      241 aagaaagcgc agtcacgtca cattggggag cagtgatgac gaagacgttg cgtgaagcat
      301 tgctggcact gctgatgatc gcgttcgccg tcccgaacgc gggcgcgacg tcgcgcatca
      361 aggacctcgc caatatcgag ggcgtgcggc agaaccagct catcggctac ggcctggtgg
      421 tcgggctcaa cggcaccggc gacaccctca acaatattcc cttcaccaag cagtcgctca
      481 ccgcgatgct ggaacggctc ggcgtcaaca tccgcggcca gaccctgcgc accggcaacg
      541 tcgccgccgt gatggtgacc gccaatctgc ccgctttcgg cacgcaaggc acgcgcatcg
      601 acgtcaccgt ctcttcgctt ggcgacgcca agagcctgca aggcggcatg ctgctggtca
      661 ccccgctgct cggcgccgac ggcaacgtct acgcggtcgg tcagggctcg gtggcgatcg
      721 gcggcttcca ggccgaaggc gaggccgcca agatcgtgcg cggcgtgcca acggtcggcc
      781 gcatcgcaaa cggcgcgctg atcgagcgcg aaatcgaatt taccttgaac aagcttagcc
      841 agttgcgcat ggcgctgcgc aatcccgatt tcaccaccgc caagcgggtc gccgccgcca
      901 tcaacgactt catcggcgtt ccgacggccg agccgctcga tccggccacc gtcggcatca
      961 ccattccgca gcaataccgc ggcaacgtca tctcgctgct caccgag
//
LOCUS       DASRVO010000685         1031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_253701, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000685 DASRVO010000000
VERSION     DASRVO010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253701"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1031
                     /locus_tag="VFF05_08500"
     CDS             <1..>1031
                     /locus_tag="VFF05_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HZW51888.1"
                     /translation="GESRFALSEVLDLLDVPALVRRFGLDGDDRDRLEATLRRAGVAW
                     GLDAPMKAQAGGAAVENNSWSFGFDRIFAGAIAGADTNGQLLDDVLPLPGIDASAALV
                     LGRAHRLLDSLRELRDGFAQPRRLRDWCAWLGEWVDVLFRADADDAAEGRALDALRRA
                     LAELGTQSTAAGAQKLPWSVAREAVRGQLDAVSERQPFLLGGVTFCGLVPQRSIPFRV
                     VCLIGMNEGEFPRDGADTGLNRMLEQPQRGDRDTRREDRQLFLEALMAARQHLHISYI
                     GRDAHEDRRIDPAAPLAELLQFLDECHGLSGKDKRPWLIEHPQQPFDPRYYDASDVRL
                     FSFNGRFAK"
BASE COUNT          155 a          345 c          349 g          182 t
ORIGIN      
        1 tgggcgaaag ccgcttcgcg ctgagcgaag tgctcgacct gctcgatgtt cccgcgttgg
       61 tgcgacgttt cggactcgac ggcgacgatc gcgaccggct ggaagccacg ttgcgccgcg
      121 ccggcgtggc ctggggcctc gacgccccga tgaaagcgca ggccggaggc gcggcggtgg
      181 agaacaattc ctggtcgttc ggattcgatc gcatcttcgc cggcgccatc gccggtgcgg
      241 atacgaatgg gcaactactc gacgatgtgt tgccgttgcc gggcatcgac gcgtccgctg
      301 cgctcgtact cgggcgcgcg catcgcctgc tggattccct gcgcgagctg cgcgacggat
      361 ttgcgcagcc gcgccgcctg cgcgattggt gcgcctggct tggcgagtgg gtcgacgtgc
      421 tgtttcgcgc ggacgcggac gatgccgcag agggtcgcgc gctggatgcg ctgcggcgtg
      481 cgcttgcgga actgggcacc caatccacag cggccggcgc gcagaaactt ccgtggagcg
      541 tcgcgcgcga agccgtgcgc ggccagctcg atgcggtgtc cgaacgccaa cccttcctgc
      601 tcggcggcgt gacgttctgc ggcctcgtgc cgcagcgctc tattccattc cgcgtcgtct
      661 gcctcatcgg catgaacgag ggcgagttcc cacgcgacgg cgccgacacg ggcctgaatc
      721 gcatgctcga acaaccccag cgcggcgacc gcgacacgcg tcgcgaggat cgccagcttt
      781 ttctcgaagc cctgatggca gcgcgccagc acctgcacat cagctacatc ggccgggatg
      841 cgcacgagga tcggcgcatc gatcccgccg cgccgctggc ggagttgctg caattcctgg
      901 acgagtgcca cggcctgagc ggaaaggaca agcgtccttg gctgatcgaa catccgcagc
      961 agccgttcga tccgcgctat tacgacgcat ccgatgttcg cctgttttct ttcaatggcc
     1021 gcttcgcgaa a
//
LOCUS       DASRVO010000686         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_306545, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000686 DASRVO010000000
VERSION     DASRVO010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306545"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFF05_08505"
     CDS             <1..276
                     /locus_tag="VFF05_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZW51889.1"
                     /translation="NLEKPLIDAVFALPLPATGKSEPGMVALHDDVYALFQLDAVRDG
                     DPSKLDPRTREAALNQLRQGLGYEAVSGFIDSLRKAAKIEIVENRLP"
     gene            complement(302..1084)
                     /locus_tag="VFF05_08510"
     CDS             complement(302..1084)
                     /locus_tag="VFF05_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HZW51890.1"
                     /translation="MGFLQGKRALITGIISHRSIAWGIAEAMHREGAQLAFTYVNDKL
                     KPRVDEAAKTFGSDIVLACDVSSDAQIEQLFTDLGKRWDGFDILVHSIGFAPGEALAG
                     EFLDGLTRENFRIAHDISAYSLPALTKAARPLMKGRNGAVLTLTYLGAERALANYNVM
                     GLAKASLEAGVRYLAYNLGPEGTRVNAISAGPIKTLAAAGVANFRKMLEHAEQNAPLR
                     RSVSIEEVGNAAAFLCSDLASGITGEVTYVDAGYNILGMTGL"
     gene            complement(1141..>2611)
                     /locus_tag="VFF05_08515"
     CDS             complement(1141..>2611)
                     /locus_tag="VFF05_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZW51891.1"
                     /translation="RGGALRMNRVGESKRRSGRHFQPTRDWRMAVLGSGCTALGAACL
                     LLAACSNAPRKPSPAAQEPDPAAATARVESHPSPPATATPAAELSPWAQLSSRFELPD
                     CDYSPDVLRLARWFSADPTGFAESLRQALPFLLYVAQQAEQRGMPGEFALLPYIESNY
                     VPLASSGDRAAGIWQLLPDTAREAGVRIRPDYDGRLDIVASTDAALDLLERYFDRFGD
                     WRVADMAFNAGPYHIAKLLGDSDVPAAAADLGRLRVPAHTHRHLAKLLAVACVVSDPS
                     RFNVLLPEPAPTDLLTMVSLPAPLDLQLAARMAGMDASRLRHLNPGYVRGRMPSAGPF
                     QLLLPTAQGRTLTAAMDKLPQPLWREWHEIALQQSEALDVLAQAHDIDTGALATANAL
                     AADAVLAPGTHVLVPGRGAGAAAVGAALGHTLENAETHIVRAGDTLWGIARSARVALS
                     DLLHWNGLSRHSTLRLGQRLRLQAPDRGGATATAAASPD"
BASE COUNT          459 a          833 c          897 g          422 t
ORIGIN      
        1 aatctggaaa agccgctgat cgacgcggtg ttcgcgctgc cgttgcccgc gaccggcaag
       61 tccgaaccgg gcatggtcgc gctccatgac gacgtctatg cattgttcca gctcgatgcg
      121 gtcagggacg gcgatccgtc caagctcgat ccgcgcacgc gcgaagccgc gctgaaccag
      181 ctgcgccagg gcttgggtta tgaagccgtg agcggtttca tcgacagcct gcgcaaggcg
      241 gcgaagatag agatcgtcga aaaccggctc ccataagggg ccggtttttc ctgcgcgact
      301 ctcaaagtcc ggtcatgccc aggatgttgt agcccgcatc cacgtacgtc acttcgccgg
      361 tgattcccga agccaggtcc gaacacagga acgcggcggc attgccgact tcctcgatgc
      421 tcacgctgcg acgcagcggc gcgttttgtt ccgcgtgttc cagcatcttg cgaaagttcg
      481 cgacgcccgc ggcggccagg gtcttgatcg gtccggccga aatcgcgttc acgcgggtac
      541 cttccgggcc gaggttgtag gcgagataac gcacgccggc ttcaagactg gccttggcca
      601 ggcccatcac gttgtaattc gccagtgcgc gttcggcgcc aagatacgtg agcgtgagca
      661 cggcgccgtt gcgccccttc atcagcggcc gcgcggcctt ggtcagcgcc ggcaggctgt
      721 aggcggaaat gtcgtgtgcg atccggaaat tttcgcgcgt caggccgtcg aggaattcgc
      781 ccgccagcgc ctcgcccggt gcgaatccga tcgaatggac caggatatcg aaaccgtccc
      841 agcgcttgcc gagatcggtg aacaactgct cgatctgcgc gtcgctggac acatcgcacg
      901 ccagcacgat gtccgagccg aacgtcttgg cggcctcgtc gacgcgtggc ttgagcttgt
      961 cgttgacgta ggtgaaggcg agctgtgcgc cttcgcggtg catggcttcg gcgatgcccc
     1021 aggcgatgga acgatggctg atgatgccgg tgatgagtgc gcgcttgccc tgcaaaaatc
     1081 ccatgatgaa tcccgtcgaa tggtcgaaac ggggattcta gccgaccgcg cggaaccgcg
     1141 tcagtcgggg ctggccgctg ccgtggccgt cgcaccgccc cgatccggcg cctgcaggcg
     1201 caggcgctgg cccagacgca acgtggagtg ccgcgacaga ccattccaat gcagcaggtc
     1261 ggacagcgca acacgcgcgg agcgggcaat tccccacagc gtgtcgccgg cacgcacgat
     1321 atgcgtttcg gcattttcca aggtgtgccc aagcgcggcg ccaacagcag ccgcgccggc
     1381 accgcgtccg ggtacgagaa cgtgcgttcc cggcgcgagc accgcatccg cggccagtgc
     1441 atttgcggtg gcgagcgcgc cggtgtcgat gtcgtgggcc tgcgccagca cgtccagtgc
     1501 ttcggattgt tgcaaggcga tctcgtgcca ttcgcgccac aacggctgcg gcagcttgtc
     1561 catggccgcc gtcaaagtcc ggccctgcgc cgtcggcagc agcaactgga aagggccggc
     1621 ggatggcatg cgcccgcgta cgtaacccgg attgaggtgg cgcagccgtg aggcgtccat
     1681 gccggccatg cgcgcggcga gttgcaggtc cagcggcgcc ggcagggaaa ccattgtcag
     1741 caaatcggtc ggggcgggct cgggaagcag cacattgaag cgcgagggat ccgaaacgac
     1801 gcaggccacg gccagcaatt tggccaggtg ccggtgcgtg tgcgcaggaa cgcgcaggcg
     1861 cccgaggtct gccgcagcgg ccgggacgtc ggaatcgccc agcaatttgg cgatgtgata
     1921 cgggccggca ttgaacgcca tgtcggccac gcgccaatcg ccgaaccgat cgaagtagcg
     1981 ttcgagcaag tcgagcgcgg catcggtgga cgcaacgatg tccaggcgtc catcgtaatc
     2041 ggggcggatg cgaacgccgg cctcgcgcgc ggtatccggc agcaattgcc agatgccggc
     2101 ggcacggtcg ccgctggaag cgagcggcac gtaattgctt tcgatgtagg ggagcaaggc
     2161 gaattccccc ggcatgccgc gttgctcggc ctgctgcgcg acgtacagca ggaacggcag
     2221 tgcctggcgc aaggactcgg caaagccggt cgggtcggca ctgaaccagc gcgcgagacg
     2281 caagacgtcc ggactgtagt cgcaatccgg cagctcgaaa cgactgctca gctgcgccca
     2341 tggcgaaagc tcggcggcag gcgtggcagt ggcggggggc gatggatggg attcgacgcg
     2401 agcggttgcg gccgcaggat cgggttcctg cgcggcaggc gacggtttgc gtggagcgtt
     2461 gctgcacgcg gcgagcaaca ggcacgccgc acccagcgcc gtgcagccgc tgcccaatac
     2521 cgccatccgc cagtcgcgcg tcggctgaaa atgccggcct gagcggcgct tggattcgcc
     2581 aacccggttc atccgtagcg cccctccccg g
//
LOCUS       DASRVO010000687         1782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_280553, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000687 DASRVO010000000
VERSION     DASRVO010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280553"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFF05_08520"
     CDS             complement(<1..568)
                     /locus_tag="VFF05_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HZW51892.1"
                     /translation="MTYRGRFAPSPTGSLHFGSLVAAVGSWIRARAQNGAWIVRMEDL
                     DPPREVPGAAADILETLAAFGMESDEAVLFQSRRESAYAAAFARLDAAGHVFPCWCSR
                     TDLESIGGIHHACVALPDATRPPAWRLRDDAGDFVVRRVEGWYAYQLAVVVDDAEQGI
                     TEVVRGADLLESTPRQIHLQRLLGYPTPE"
     gene            640..1521
                     /gene="htpX"
                     /locus_tag="VFF05_08525"
     CDS             640..1521
                     /gene="htpX"
                     /locus_tag="VFF05_08525"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HZW51893.1"
                     /translation="MLRILLFLGTNLAIMLVLGIVCSIFGIDQWAYGKAGINLQGLLV
                     MCAVFGMGGAFISLAMSKTIAKWTTHAQVIEQPRGANEQWLLDTVRQHAQQAGIGMPE
                     VALYDSPDMNAFATGMSRNHALVAVSTGLLQSMNRDEISAVLGHEITHVANGDMVTLT
                     LIQGVLNTFVMFLARIIGSVVDSAIGGNRDDNRGGGIGYFLIVMLLQTVLGFLATIVV
                     MWFSRWREFRADAGGARLGSRAGMIAALQTLSGSHESGLPKAVQAFGISGDSGVARLF
                     MSHPPIAERIAALKAQG"
     gene            complement(1577..>1782)
                     /locus_tag="VFF05_08530"
     CDS             complement(1577..>1782)
                     /locus_tag="VFF05_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HZW51894.1"
                     /translation="IGGKIAKIVNENTLYGQPYVLNTEQTVEAAAKAAGADVIGFKRL
                     VVGEGIEKVQEDYVAEVKKAMGG"
BASE COUNT          310 a          580 c          562 g          330 t
ORIGIN      
        1 attccggcgt cggatagccg agcaggcgtt gcagatggat ttgccggggt gtggattcga
       61 gcaagtccgc gccacgcacg acttcggtga tgccttgctc cgcatcatcg acgaccacgg
      121 cgagctgata cgcgtaccag ccttcgacgc gacgcacgac gaaatcgccc gcgtcgtcgc
      181 gcagacgcca cgcgggcggg cgcgtggcgt cgggtaatgc gacgcaggcg tgatggatgc
      241 cgccgatcga ttccagatcc gtgcggctgc accagcatgg aaaaacgtgt ccagccgcat
      301 ccaggcgcgc gaacgcggcc gcatacgcgg actcgcgccg gctttggaac agcaccgcct
      361 cgtcggattc catgccgaag gcggcgaggg tttcgaggat atccgccgcc gcgccgggaa
      421 cctcacgcgg cggatcgagg tcttccatac ggacgatcca ggcgccgttt tgcgcacgcg
      481 cgcgaatcca gctcccgacc gcggcgacca gcgaaccgaa atgcagcgat ccagtaggcg
      541 aaggggcaaa acggccgcgg taagtcatgc tctacactgc ttgaattcgg ttcagtttac
      601 ccatagctat tccacgatcc gccaaccacg aggaattcca tgctgcgcat tctgctgttt
      661 ctcggcacca acctcgccat catgctggtg cttggcatcg tctgctcgat cttcggcatc
      721 gatcagtggg cgtatggcaa ggccggcatc aacctgcaag gcctgctggt catgtgcgcc
      781 gtgttcggca tgggcggcgc gttcatttcg ctggcgatgt ccaagaccat cgcgaagtgg
      841 accacccacg cgcaggtgat cgagcagccg cgcggcgcga acgaacaatg gctgctcgac
      901 acggtgcgcc agcacgcgca gcaagccggc atcggcatgc cggaggtcgc gttgtacgac
      961 tcgccggaca tgaacgcgtt cgcgaccggc atgagccgca atcatgcgct ggtcgcggtc
     1021 agcacgggcc ttttgcaatc catgaaccgc gacgagattt ccgcggtgct cggccacgag
     1081 atcacgcacg tcgccaatgg cgacatggtc acgctcaccc tgatccaggg cgtgctcaac
     1141 acgttcgtga tgttcctcgc gcgcatcatc ggcagcgtgg tcgacagcgc catcggcggc
     1201 aatcgcgacg acaaccgcgg cggtggcatc ggctatttcc tgatcgtgat gctgctgcag
     1261 accgtgctcg gtttcctcgc caccatcgtc gtcatgtggt tctcgcgctg gcgcgaattc
     1321 cgtgccgacg cgggcggcgc acgccttggc agtcgcgccg gcatgattgc ggcgctgcaa
     1381 acgctgtccg gcagccatga atcgggcctg ccgaaagccg tgcaggcgtt cggcatctcc
     1441 ggcgacagcg gcgtggcgcg cctgttcatg agccatccgc cgattgccga gcgcatcgcg
     1501 gcgctgaagg cgcagggctg aaaacaaaaa agccgcgatt gctcgcggct ttttttgttc
     1561 gagcgcagca gcttcttcag ccgcccatcg ccttcttcac ttcggcgacg taatcttcct
     1621 gcaccttctc gatgccttcg ccgacaacca ggcgcttgaa gccgatcacg tcggcgccgg
     1681 cggccttggc cgcggcttcg acggtctgct cggtgttgag cacgtagggc tggccgtaca
     1741 gcgtgttctc gttgacgatc ttggcgatct tgccgccgat ga
//
LOCUS       DASRVO010000688         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_202455, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000688 DASRVO010000000
VERSION     DASRVO010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202455"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1677)
                     /locus_tag="VFF05_08535"
     CDS             complement(37..1677)
                     /locus_tag="VFF05_08535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HZW51895.1"
                     /translation="MKASDLFVKALEAEHVHYVFGIPGEENLDLLESLRTSSIKLILT
                     RHEQAAGFMAATYGRLTGKAGVCLSTLGPGATNFVTAAAYAQLGGMPMLMLTGQKPIK
                     VSKQGHFQIVDVVDMMKPLTKYTRQIISADNIPARVREAFRRAEEERPGATHLELPED
                     IAHDPTDAPLIHASYHRRPIADEKCIAHAVDAICAARHPLLMIGAAANRKLCSKMLRE
                     FVDSTGIPFFTTQMGKGVLDETHPLWLGNAALSDGDFVHRAIERADCIVNVGHDVIEK
                     PPFFMRTGKRTVIHVNFASAEVDTVYFPQIEIIGDIANSVWRLKQRIAPCPHWDFTYF
                     SSVREHLDAHLAQGADDARWPIYPQRLVADVRKVVPSDGILCLDNGMYKIWFARYYRC
                     REPNTLLLDNALATMGAGLPSAIAAKIVHPHRKVIAVAGDGGFMMNSQEIETAVRLKL
                     DLIVLLLRDNAYGMIKWKQTNLSFPTYGLDLGNPDFVAYAQSYGAHGHRPSSAAEFAP
                     LLAKCLDTPGVHLIDVPIDYSDNDRILNREIKELSAKV"
BASE COUNT          291 a          514 c          599 g          349 t
ORIGIN      
        1 tgagcatggc aatgttccct tcgtcggcgg tcagcatcaa accttggcgc tgagttcttt
       61 gatctcccgg ttcaagatcc ggtcgttgtc ggaatagtcg atcggcacgt cgatcaggtg
      121 cacgcctggc gtgtcgaggc actttgccaa cagcggtgcg aattccgcgg cggacgacgg
      181 gcgatggccg tgcgcgccgt agctttgcgc gtaggcgacg aaatccgggt tgcccaagtc
      241 cagtccgtag gtggggaaac tcaggttggt ctgtttccac ttgatcatgc cgtaggcgtt
      301 gtcgcgcagc agcagcacga tcaggtcgag cttgaggcgc accgcggttt cgatttcctg
      361 cgagttcatc atgaaaccgc cgtcgccggc gaccgcgatt actttgcggt gcgggtgcac
      421 gatcttcgca gcgatggccg agggcaggcc cgcacccatc gtcgccagcg cgttgtcgag
      481 cagcagcgtg ttcggctcgc ggcagcggta gtagcgcgca aaccagattt tgtacatgcc
      541 gttgtccagg cacaggatgc cgtcggacgg cacgaccttg cgcacgtcgg cgacgaggcg
      601 ctgcggatag atcggccagc gcgcgtcgtc cgcgccctgc gccaggtgcg cgtcgaggtg
      661 ttcgcgcacg ctggaaaaat aggtgaaatc ccagtgcggg cagggcgcga tgcgctgctt
      721 gagccgccac acggaattgg cgatgtcgcc gatgatttca atctgcggga agtagaccgt
      781 gtcgacctcg gcgctggcga aattgacgtg aatgaccgtg cgcttgccgg tgcgcatgaa
      841 gaacggcggt ttctcgatca cgtcgtggcc gacgttgacg atgcagtcgg cgcgctcgat
      901 cgcgcggtgc acgaagtcgc cgtccgacag cgccgcattg ccgagccaca gcggatgcgt
      961 ctcgtcgagc acgcccttgc ccatctgcgt ggtgaagaac ggaatgccgg tgctgtcgac
     1021 gaattcgcgc agcatcttgc tgcacaactt gcggttggcc gccgcgccga tcatcagcag
     1081 cggatggcgc gcggcgcaga tcgcgtcgac cgcgtgcgcg atgcatttct cgtcggcgat
     1141 cggacggcgg tggtagctgg catggatcag cggcgcgtcg gtcgggtcgt gggcgatgtc
     1201 ctcgggcagt tccaggtgcg tcgcaccggg gcgttcttcc tcggcgcggc ggaatgcctc
     1261 gcgcacgcgc gccggaatgt tgtcggccga aatgatctgc cgcgtgtact tggtcagcgg
     1321 cttcatcatg tcgaccacgt cgacaatctg gaaatgacct tgcttgctca ccttgatcgg
     1381 cttctgaccg gtgagcatga gcattggcat gccgccgagc tgggcgtagg cagcggcggt
     1441 gacgaagttg gtcgcgcccg gtccgagcgt ggacaggcac acgccggcct tgccggtgag
     1501 gcgcccgtat gtggcggcca tgaaaccggc ggcctgttcg tgacgggtga ggatcagctt
     1561 gatcgaggag gtacgcagcg attcgagcag gtcgaggttt tcctcgccgg gaatgccgaa
     1621 cacgtaatgc acgtgctcgg cttcgagcgc cttgacgaac aggtccgaag ccttcatcgc
     1681 gtttcccctt gtgaagatgc cggattgtcc agccatgcgc ttgatgcgtc aaatgcgcaa
     1741 tttctaggcg acc
//
LOCUS       DASRVO010000689         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_20277, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000689 DASRVO010000000
VERSION     DASRVO010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20277"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..812
                     /gene="lepB"
                     /locus_tag="VFF05_08540"
     CDS             27..812
                     /gene="lepB"
                     /locus_tag="VFF05_08540"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZW51896.1"
                     /translation="MSATEGSAVERQQRAQEALREPILVEYARSFFPVILIVLLFRSF
                     IAEPFKIPSGSMMPTLLVGDFILVNKFAYGLRLPVLGTKLVDIGEPHRGDVFVFKYPG
                     FSCVVDGKPVRSGKPCEYPLEPVPKLDYIKRVVGLPGDTITYRNKTLYVNGQEIVQAD
                     VGPFVGTGPEGRRMAGAEIHTEELSGVRHDVLESNFLPPGREGTWVVPAGHYFAMGDN
                     RDNSEDGRYWGFVPEKNLIGKAFVIWMNFDGGIDFKRIGTLIK"
     gene            821..1201
                     /locus_tag="VFF05_08545"
     CDS             821..1201
                     /locus_tag="VFF05_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008215679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HZW51897.1"
                     /translation="MRKQHGITLIGFAIVLIVVGFFAYAAMKLVPVYSEYFGVVKSLK
                     SLQSDAGIQNMGIEEIRRKLDTIFDVQYVDETDVPLSAVTLITANGQHSLNVTYSVQK
                     PFIYNIDLLVHFDYTVDLSKGATY"
     gene            1204..1866
                     /gene="rnc"
                     /locus_tag="VFF05_08550"
     CDS             1204..1866
                     /gene="rnc"
                     /locus_tag="VFF05_08550"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZW51898.1"
                     /translation="MGAAFDYGFRDPALRELALRHRSTGARNNERLEFLGDALVGLFA
                     AELLYSRFPHADEGELTRWRARMVNEGALAQIARELDLGDQLHLGPGELKSGGFRRDS
                     ILADAFEALVGACYLDGGYDACRHVVRAIFEPRVDAATRAQEKDPKTQLQELLQGRGL
                     PLPAYELTGSSGADHAKVFEVTCAIAAFDLRCTGHGSSRRAAEQAAATAALERIAEAR
                     HV"
     gene            1859..>2146
                     /locus_tag="VFF05_08555"
     CDS             1859..>2146
                     /locus_tag="VFF05_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZW51899.1"
                     /translation="MSETHPADFRCGTAALVGRPNVGKSTLLNALLGWHLSIAGPKPQ
                     TTRHRILGILTRPHGQILFLDTPGMHRSGGRAINRQLNRAARQAIAEADVAV"
BASE COUNT          388 a          692 c          680 g          386 t
ORIGIN      
        1 gcggacgcgc ggctcgcgcg caggcgctga gcgcgactga aggcagcgcg gtggagcgcc
       61 agcagcgcgc ccaggaagcg ctgcgcgaac cgattctggt cgagtacgcg cgatcgttct
      121 tcccggtcat cctgatcgtg ctgctgttcc gctcgttcat cgccgaaccg ttcaagattc
      181 cgtccggatc gatgatgccg accctgctcg tcggcgattt catcctggtc aacaagttcg
      241 cctacgggct gcgcctgccg gtgctgggta ccaagctcgt cgatatcggc gaaccgcatc
      301 gcggcgacgt gttcgtgttc aagtatcccg ggttttcctg cgtggtcgac ggcaaaccgg
      361 tgcgcagcgg caaaccctgc gaatatccgc tggaaccggt gccgaagctg gattacatca
      421 agcgcgtggt cggcctgccg ggcgacacca ttacctatcg caacaagacc ttgtacgtga
      481 acggtcagga aatcgtgcag gccgatgtcg gacccttcgt cggcacgggg cccgagggcc
      541 ggcgcatggc cggtgcggaa atccacacgg aggaactctc cggcgtgcgc cacgatgtgc
      601 tggagtcgaa tttcctgccg ccgggccgcg aaggcacctg ggtggtgccc gctggacact
      661 atttcgcaat gggggataat cgcgacaaca gcgaagacgg ccgctactgg ggcttcgtgc
      721 cggagaagaa cctgatcggc aaggccttcg tgatctggat gaacttcgac ggcggcatcg
      781 acttcaagcg aatcggcacc ttgatcaaat gaggacgacc atgcgcaagc aacacggcat
      841 caccctgatc ggctttgcca tcgttctgat cgtcgtcggg ttcttcgcct acgcggcgat
      901 gaagctggtt ccggtctatt ctgagtattt cggcgtggtc aagtcgctga agtctttgca
      961 gagcgacgcc ggcatccaga acatgggcat cgaggagatc cggcgcaagc tcgacacgat
     1021 tttcgacgtg cagtatgtgg atgaaaccga tgtgccgttg tcagccgtga ccttgatcac
     1081 cgccaacggc cagcacagcc tcaatgtcac ctacagcgtg cagaagccgt tcatctacaa
     1141 catcgacctg ctggtacatt tcgactatac cgtcgacctg tccaaaggcg cgacgtactg
     1201 agtttgggcg ccgccttcga ctacggcttt cgcgatccgg cgctgcgcga actggcgcta
     1261 cgccaccgca gcaccggcgc gcgcaacaac gaacggctgg aattcctcgg cgacgcgttg
     1321 gtcggactgt ttgccgcgga gttgctctac tcccgtttcc cgcacgcgga cgagggcgaa
     1381 ctcacgcgct ggcgcgcgcg catggtcaac gaaggcgcgc tggcgcagat cgcgcgcgaa
     1441 ctcgaccttg gcgaccagtt gcatctgggg ccgggcgagc tgaaaagcgg cggattccgg
     1501 cgcgattcca tcctcgccga cgcgttcgaa gccttggtcg gcgcctgtta cctggacggc
     1561 ggctacgacg cctgtcgcca cgtcgtgcgc gcgattttcg agccccgtgt cgatgcggcc
     1621 acccgcgcgc aggaaaagga tccgaagacg cagttgcagg aattgctgca aggacgcggc
     1681 ctgccgctgc cggcctacga actgaccgga tccagcggcg ccgatcacgc aaaggtcttc
     1741 gaggtgactt gcgccattgc cgctttcgat ctgcgctgca cgggccacgg cagcagccgg
     1801 cgtgcggccg agcaagccgc ggcgacggcg gcattggaac gaattgccga ggcgcgccat
     1861 gtctgaaacg catcccgcgg atttccgctg cggcacggcc gcgctggtcg gccgcccgaa
     1921 tgtgggcaag tcgaccttgc tcaacgcctt gctcggctgg catctgagca tcgccgggcc
     1981 caaaccgcaa accacgcgcc atcgcatcct cggcatcctc acgcgtccgc acgggcaaat
     2041 cctgtttctc gatacgcccg gaatgcaccg cagcggtggc cgcgcgatca atcgccagct
     2101 caaccgggcc gcgcgccaag ccatcgccga ggccgatgtt gcggtg
//
LOCUS       DASRVO010000690         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_149217, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000690 DASRVO010000000
VERSION     DASRVO010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149217"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..901
                     /locus_tag="VFF05_08560"
     CDS             131..901
                     /locus_tag="VFF05_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51900.1"
                     /translation="MVASRLMARLREHDWMAAAVELVIVVVGILIALQVSNWNQDRID
                     RNRAQGYYRRIHADLSVDLKNMDATLAFWRAVSAYGDAAIADGEGKPADETPGWTTLL
                     AWYQASQTMPFVASDAAFGEMRSAGELGLIADEGLRKRLDDYYSLSGVGGQSIIREQY
                     PEYRLQIRGLTPWHVQQYIWSRCFSESSFIAQQFVDCNAPIDAQEASAILRSYRDSPA
                     LLNQLRAWMSTLRISEIVLVNDRRDAEQLDAAVTKAMK"
     gene            complement(1445..>1731)
                     /locus_tag="VFF05_08565"
     CDS             complement(1445..>1731)
                     /locus_tag="VFF05_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51901.1"
                     /translation="HPGGFTPLLFVLSLLPFSVLLTRTFNAAAGSVLLPHLLHQSINS
                     WGESLPFLPRFAHSLAPLTISVVILIVLSALVVVVRPPMWRKLVVPEAVR"
BASE COUNT          340 a          565 c          536 g          290 t
ORIGIN      
        1 ctgccgcacg atcgcctgag atgcacaagc ctgtttcgta gtatcgtcac gccatcgaat
       61 gagtcctgcc atgaaaccgc tgatatccct cgccgctgcc gccgcacaaa tagtcgcaag
      121 gtaaatggtg atggttgcat caaggctgat ggcgcgcctg cgcgaacacg actggatggc
      181 tgctgccgtc gaactcgtca tcgtcgtcgt cggcatattg attgccctgc aagtcagcaa
      241 ctggaaccag gatcgcatag accgcaatcg cgcccagggc tattaccgcc gcatccatgc
      301 cgacttgagc gtggacctca agaacatgga tgcgaccctg gcgttctggc gtgcggtgtc
      361 cgcctacggc gacgccgcca ttgccgatgg cgaaggcaag cccgccgacg aaacgccggg
      421 ctggacgacg ctgctggcct ggtaccaggc cagccagacc atgccgttcg tggcatccga
      481 tgccgcgttc ggcgaaatgc gcagcgctgg cgagctcggc ctgatcgccg acgaaggttt
      541 gcgcaagcgg ctggacgact actactcgct ttcgggtgtc ggcggccagt cgatcatccg
      601 cgaacagtat cccgaatacc gtctgcagat ccgcggactc acgccgtggc acgtacagca
      661 gtacatctgg agccgctgct tcagcgagtc cagcttcatc gcccagcaat tcgtcgattg
      721 caatgcaccc atcgacgcgc aggaagcatc cgcgatcctc aggtcctacc gcgattcgcc
      781 ggcactgctg aatcaattgc gcgcctggat gtcgacgctg cggatcagcg aaatcgtgct
      841 cgtcaacgac cgccgcgacg ccgaacaact cgatgccgcg gtaaccaagg caatgaagtg
      901 aattgactcc gagttacctg atcggctagg ccggcagcgc acgcatgccc ggtcgcggtt
      961 gtagatgcgc ggcgcatcgt ccacgctcat ggggcgaatc cccctcgcgg cggcgcccgg
     1021 cgcgcctgcg cgaacagcgc cgtcggcgct aggcccggca accgcgtaca ggcgacctct
     1081 ggacgcaagg cgcgcggtgt cggcaagcgc gtatcgtgta cgtcgccacc gtaccgtcgc
     1141 cgtagagcgt gtgcaggttg acctgcgcgc cgatcgacga cgtcgtttcg accgcggcga
     1201 ggaaattctc accggtgtgc accatcagtg tcagcttgat ctatagcgcg acgccagcgc
     1261 gccgagcagg tagacagccc aaatcacaat ctggatctgc attttcccag ccgttgtcga
     1321 cgtcccttgg ctctcgttgt gcgccggatt ccagcgcggg caacgcgcac ccgtagccgc
     1381 tgtcgacggg cctgccatct aatttccccg ccaacgcgag atcgtggcgc aggcgaatgc
     1441 ggaatcagcg caccgcttcc gggactacga gcttgcgcca catcggcggg cggacgacca
     1501 caaccagcgc cgacagcacg atcaggatca ccacgctgat cgtcagcggt gccaggctgt
     1561 gcgcgaaacg cggcaggaag ggcagcgatt cgccccagct gttgatcgac tggtgcagta
     1621 gatgcggcaa caaaacgctg ccggcggccg cattgaaggt gcgcgtcagt agcaccgaga
     1681 acggcagcag cgacaggacg aacagcaagg gggtgaagcc gccgggatgc c
//
LOCUS       DASRVO010000691         1574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_124437, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000691 DASRVO010000000
VERSION     DASRVO010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124437"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VFF05_08570"
     CDS             complement(<1..395)
                     /locus_tag="VFF05_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51902.1"
                     /translation="MSSANRVTLGEALRTLPLAAPPGDEWTRLRERLQPLQTRPSAWR
                     RYALPAALAATLAGAFLILRTPPASAPATIAHVGASSSSNGAQAVQIHNVQNAANQPD
                     RLAQLQQRSQALERWLRETGRDAAPLQGQ"
     gene            complement(374..961)
                     /locus_tag="VFF05_08575"
     CDS             complement(374..961)
                     /locus_tag="VFF05_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZW51903.1"
                     /translation="MSTSAFQIDLPSALIERVKSGDTAAFEQIYRRFERPVYSLALRM
                     LGDAEAARDALHDAMLKAFQRIAQYRGDAPFWAWLRQIAMNEALMRLRGGERFTVSME
                     SVGVDSDALADDAPAPWAHADARMLEHALAQLPALTRSVLWLYHVEGFTHPEIAAAMR
                     KSTSFSKSQLARGTARLRTLLDPEPEPLPCLLPTA"
     gene            1184..>1574
                     /locus_tag="VFF05_08580"
     CDS             1184..>1574
                     /locus_tag="VFF05_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HZW51904.1"
                     /translation="MAIKDTSSQDRVITPRVDGKRRAMQIGAAVVAVALFAWIAPSAW
                     RLFAAGRSVSAARLQIATVERGPFVRDIAAEGSVVAAVSPTLYASAPGAVVLKVHAGD
                     AVTKGEVIAVIDSPELTNKLAQEQSNAD"
BASE COUNT          250 a          513 c          546 g          265 t
ORIGIN      
        1 tcctggcctt gcagcggtgc ggcgtcgcgt ccggtttcgc gcagccagcg ttccagcgcc
       61 tgcgagcgct gttgcagttg tgcgaggcga tcgggttgat ttgccgcatt ttgcacattg
      121 tggatttgta ctgcctgtgc gccattcgag gatgaagatg cgccgacgtg cgcaatggtc
      181 gcaggcgcgg atgcaggtgg cgtgcgcagg atgaggaaag cgccggccag cgtcgccgcc
      241 agcgcggcgg gcagggcgta gcggcgccat gcggacggac gcgtctgcaa aggctgcagg
      301 cgctcgcgca gtcgcgtcca ttcgtcgccc ggcggcgccg ccagcggcag ggtgcgcagc
      361 gcttcgccca gggtcacgcg gttggcagaa gacatggcag gggctccggt tcgggatcga
      421 gcagcgtgcg caggcgtgcc gtgccgcgcg cgagttgcga tttggaaaag ctcgtcgact
      481 tgcgcatcgc ggcggcgatt tccggatgcg tgaagccttc gacgtgatac agccacagca
      541 cgctgcgcgt gagcgccggc agttgtgcga gcgcgtgttc gagcatgcgc gcgtccgcat
      601 gcgcccacgg tgccggcgca tcgtcggcga gcgcgtcgga gtcgacgccg accgactcca
      661 tgctcacggt gaagcgctcg ccgccacgca gccgcatcag cgcctcgttc atcgcgatct
      721 ggcgcagcca cgcccagaac ggcgcgtcac cacgatattg cgcgatgcgc tggaaggcct
      781 tgagcatcgc atcgtgcagc gcgtcgcgcg cggcttccgc gtcgccgagc atgcgcaagg
      841 cgagcgagta gaccggtcgt tcgaagcggc gataaatctg ctcgaacgcc gccgtgtcgc
      901 cgctcttgac gcgttcgatc agcgctgagg gcaggtcgat ctggaatgcg gatgtgctca
      961 ccatctgcat tttgatgcac gagcgcgaag aaaggtcgca gcgatctttt cggcgcgttc
     1021 gccgcactgt ccgcagcctg tccgcggaca ctttcccgga agtcgacgca tgcgcaacta
     1081 cctgacgatc atgcattttc aggcttcatc gccgctggca cagcgcttgc tctagtcgtg
     1141 tcgacgccgc aggcagcggc ttggacgaaa cggcaacgac gacatggcaa tcaaggacac
     1201 atcctctcag gaccgagtaa tcacgccacg cgtggacggc aaacgccgcg ccatgcagat
     1261 cggcgctgcc gtcgtcgccg ttgcgctgtt cgcgtggatc gcgccgtcgg cctggcgctt
     1321 gttcgcggcg ggccgctcgg tgagcgccgc gcgcctgcag atcgccacgg tcgagcgcgg
     1381 cccgttcgtg cgcgacatcg ccgccgaagg cagcgtcgtc gccgcggtga gccccaccct
     1441 gtacgcctct gcgccaggcg cggttgtgct caaggtgcac gcgggcgacg cggtgaccaa
     1501 gggcgaggtc atcgccgtca tcgacagtcc cgaactgacc aacaagctcg cgcaggagca
     1561 atccaacgcc gatg
//
LOCUS       DASRVO010000692         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_383651, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000692 DASRVO010000000
VERSION     DASRVO010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383651"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VFF05_08585"
     CDS             complement(<1..827)
                     /locus_tag="VFF05_08585"
                     /inference="COORDINATES: protein motif:HMM:NF022800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HZW51905.1"
                     /translation="MLASLTNANRARSSRLAAMGACAAAALACLWLLVRLAWLLVPQG
                     DVASASTPVASTPVAVPVQSVGKWHLFGNPQTIAVLQQMRNAPATTLKLTLRGTLAMD
                     DAHEGIAMIEDEHGGERAYKVGEVVGSNAKLVAVYADHVVLDHEGVAETLNLPRADQH
                     APPPPPANQANPATATGARASSIPPNYAPPQLSGNLDWSAAQSKLRVDPAQLAKQVHV
                     EPVFENGKIAGARLSGGGEVARLMNQAGLKSTDLITAINGQSLSAVSNPQQFMDNLAG
                     "
     gene            complement(873..>1682)
                     /locus_tag="VFF05_08590"
     CDS             complement(873..>1682)
                     /locus_tag="VFF05_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HZW51906.1"
                     /translation="KVAAEKVSRRLERHGFLVGVLSGDVPQKKRQSLLGKFQRGEIEI
                     LIATDVAARGLHIPDVTHVVNYDLPMDAEDYVHRIGRTARLGADGDAISFACDMYAQG
                     LPDIEAYIGQKIPVLRVEPELLVIPPPRAPVVSAEGGDGESNIDTSARKPHAPRARPG
                     DNRRPHRDKPREHAHKPAPTAPVAAPAHPAPASGDANAPARKRRRGGRGRHRGEGADA
                     ATRNRDGANPAPEKRMRGHQPVPTKPVAAPSPAGHKKPGFFRRLGNLFKRS"
BASE COUNT          238 a          547 c          594 g          303 t
ORIGIN      
        1 ccggccagat tgtccatgaa ttgctgcgga ttggacaccg ccgacagcga ctggccgttg
       61 atcgcggtga tgaggtcggt ggacttgagc ccggcctggt tcatcagccg cgccacctcg
      121 ccaccgccgg acaggcgcgc gccggcgatc ttgccattct cgaacaccgg ctcgacgtga
      181 acttgtttgg cgagttgtgc cggatccacg cgcaatttgg attgcgccgc gctccagtcc
      241 agattcccgc tcaattgcgg cggcgcatag tttggcggga tgctcgatgc gcgcgcgccg
      301 gttgccgtgg cgggattcgc ctggttggcc ggcggcggcg gtggcgcgtg ctgatccgca
      361 cgcggcaggt tcagggtttc ggccacgcct tcgtgatcga gtacgacgtg atcggcatac
      421 acggccacga gtttcgcatt gctgccgacg acctcgccga ccttgtaggc gcgctcgccg
      481 ccgtgctcgt cctcgatcat ggcgatgcct tcgtgcgcat cgtccatcgc cagcgtgccg
      541 cgcaaagtga gcttgagcgt cgtcgccggc gcgttgcgca tctgctgcaa caccgcgatc
      601 gtttgcggat tgccgaacag atgccacttg cccaccgact gtaccggcac cgccactggt
      661 gtggacgcaa ccggcgtgga tgcgctcgcg acatcgccct gcggcaccag cagccaggcc
      721 agacgcacca gcagccacag acaggccagc gcggcagccg cacacgcacc catcgcggca
      781 aggcgcgagg agcgggcacg gttggcgttg gtcagcgatg cgagcatgcg gcggcggtga
      841 aaaatcccgc cgcagacttt agcagctagg cgtcaggaac gcttgaacag attgccgagg
      901 cggcggaaga agcccggctt cttgtgcccg gctggcgacg gcgcggcgac tggcttggtc
      961 ggcaccggct ggtgtccacg catgcgcttt tcgggtgcgg gattggcgcc gtcgcggttg
     1021 cgcgtggctg catctgcgcc ttcgccgcga tggcgtccac gtccgccacg gcggcgtttg
     1081 cgcgcgggcg cgttcgcatc gccgcttgcc ggtgcgggat gggcaggcgc tgcgaccggc
     1141 gcggttggcg ccggcttgtg cgcgtgttcg cgcggcttgt cgcgatgcgg gcggcggttg
     1201 tcgcccggtc gcgcgcgcgg cgcatgcggt ttgcgcgcgg aagtatcgat attggattcg
     1261 ccgtcgccac cttcggcgct aacgacggga gcacgcggtg gcgggatcac gagcagttcc
     1321 ggttccaccc gcaagaccgg gatcttctgg ccgatgtagg cctcgatgtc gggcaggccc
     1381 tgcgcgtaca tgtcgcaggc gaaactgatc gcgtcgccgt ccgctccaag acgggcggtg
     1441 cggccgatgc gatgcacgta gtcttccgca tccatcggca ggtcatagtt gaccacgtgg
     1501 gtgacgtcgg gaatgtgcag gccgcgcgcg gccacatccg tcgcgatgag gatttcgatt
     1561 tcaccgcgct ggaacttgcc gagcaggctc tggcgcttct tctgcggcac gtcgccggac
     1621 agcacgccga ccaggaagcc gtggcgctcg agccggcgcg agactttctc ggcggcgacc
     1681 tt
//
LOCUS       DASRVO010000693         9317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_383672, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000693 DASRVO010000000
VERSION     DASRVO010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383672"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFF05_08595"
     CDS             complement(<1..1140)
                     /locus_tag="VFF05_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZW51907.1"
                     /translation="MYLKKKISPFALAVALGCGLATGYAPSLLAEGPVASAEQSNVLR
                     ATLDNGLRVVIVHDTLAPMVTTQITYMAGSYETPQGFPGTAHALEHMMFRDSKGLSGS
                     QLNEMTGKMGAENNAFTTNDATQYYFVAPAQYVDLLLKIEATRMRGAQLSDKDWGLEK
                     GAIEQEVSRDISDPGYLAFADAERALYAGTGYAEDALGTRPSFDKTTGKVLQGFYDSW
                     YVPNNALLVIAGDVDAQATLAKVKELFGSIPHHDTPARASVKLAAFKPSTISKTTPQG
                     TGSVQFMYRMPGMKSKDYAASEVLMDVLGNARSSLSELAAQGKVLSSDAGVQPFGHGG
                     IGIVEVGFPKGGDAAQAQKHLDGVIADLLKNGVSTDLVDAAKRSEL"
     gene            complement(1184..2227)
                     /gene="murB"
                     /locus_tag="VFF05_08600"
     CDS             complement(1184..2227)
                     /gene="murB"
                     /locus_tag="VFF05_08600"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZW51908.1"
                     /translation="MKPDTLSASGFRVTEDADLTRHNSFRVPARAQWLIEVDDARALP
                     EVFGLGAVQRSPLLILGEGSNVLLTRDWPGVVLSPIMRGIRILEDRGDNASVRVEAGE
                     NWNEFVHWSLGHCFAGLENLILIPGSVGAAPIQNIGAYGVEASEFIATVEAFDRRSGQ
                     LVRLANAACAFAYRDSLFKHEPDRYLVCAVEFRLPRERELRLDYAGVREELVAMKADA
                     PNHAAVAEAVMRLRLRKLPNPAEIGNAGSFFKNPLVPESQAADLQRTYPALPSWPAEH
                     GQRKLSAAWLVETAGFKGFREGDAGVSERHALVLVNRGAATGEQIRTLAQRIVDAVQA
                     RFGVRIEPEPRIV"
     gene            complement(2224..3249)
                     /locus_tag="VFF05_08605"
     CDS             complement(2224..3249)
                     /locus_tag="VFF05_08605"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZW51909.1"
                     /translation="MYALTRPFLFCLDAERAHDLALTAIEVAYRTGLNPLLAAKPAPL
                     PTIVFGIEFPNPVGLAAGLDKNGAHIDALAALGFGFIEIGTTTPRAQPGNPKPRMWRL
                     QEHQAVINRLGFNNAGVDALVHNVERARFSGVLGINIGKNKDTRNEDAVEDYRYCLER
                     VYACATYVTVNISSPNTQGLRDLQEEETLKRFIGTLRDAQEKLASRHGTRKPMLLKIA
                     PDLAEGEMDGIAQVLLTAKIDGLICTNTTIDRSAVAGARHAEEAGGLSGKPLFDKATS
                     ILRGMAQRLGGKIPLIGVGGILSGADAAAKIAAGASLVQFYSGMVYHGPALIGECVEA
                     IRKRAAR"
     gene            complement(3259..3453)
                     /locus_tag="VFF05_08610"
     CDS             complement(3259..3453)
                     /locus_tag="VFF05_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51910.1"
                     /translation="MGVLADAISVLKDVLQMRGDMDRLARNVETLTQLMTDLDRRMIR
                     IETMVEIAEKRRAPPRLSKT"
     gene            complement(3455..3769)
                     /locus_tag="VFF05_08615"
     CDS             complement(3455..3769)
                     /locus_tag="VFF05_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZW51911.1"
                     /translation="MKTERVTLLISPDDKRQLQGLAEDRGMTASELVRQAVQAYGASS
                     VDETRELAALTRELREAIPGMRRSLHDANTAAERAIAVMRGGSPQHRVAEPRARRRVG
                     KR"
     gene            complement(3837..5369)
                     /locus_tag="VFF05_08620"
     CDS             complement(3837..5369)
                     /locus_tag="VFF05_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZW51912.1"
                     /translation="MSTPILKALGLGPNNSGTYIGHGEWSKTADAGLLQSINPSTNEV
                     IAEVHASSASDYAAIVKRAQEAFATWRTTPAPRRGEAIRLCADALRKHKDALGSLVAL
                     EMGKSKPEGDGEVQEMIDIGDFAVGLSRQLYGLTMHSERPGHRMYEQWHPLGLVGVIS
                     AFNFPVAVWAWNAFVAAVCGDITLWKPSPKTPLSAIAAMRICNEALKSGGFPDIFFLF
                     NDAGTELASQFVDDKRIALISFTGSTRVGRIVGERVAQRMGRSLLELGGNNAIIVDAS
                     ADLKLAIPAIVFGAVGTAGQRCTTTRRLFVHESIFDDVLATLVAAYKQVEKKIGDPTD
                     AKNLMGPLNSRDAVNAYLGAIEKAKASGGKVATGGAAIEGKGNFVLPTIITGLKNSDE
                     VVQTETFAPILYVMKFKTIDEAITQQNAVPQGLSSSIFTQNLKAAEAFLGAAGSDCGI
                     ANVNIGTSGAEIGGAFGGEKETGGGRESGSDAWRAYMRRQTNTINYSDALPLAQGIKF
                     DF"
     gene            5486..7024
                     /locus_tag="VFF05_08625"
     CDS             5486..7024
                     /locus_tag="VFF05_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZW51913.1"
                     /translation="MSDDNPAVPSARRQIFSDRDLWILIALAAAKLAFHLATNGQYGF
                     HRDELQTLSDSHHLDWGFVAYPPLTPFFAHVEWLLFGNSLIGLRSFAALAQSIVFVVT
                     GLIARRLGGGRAAMLLAAFAVALSPVSLSASSLLMYVTFDFLCFVLLAYFVVRRIESG
                     DARWWITIGAIVGVGMMTKYTMAFFALGLAAGVFLTPLRADLRGKWPWLGMALAVLIF
                     LPNLLWLARHDFITLTFLQFIHARDVRIGRTDGFWIDQIKIAANFATVPFWLAGLWFL
                     FFAEAGRRWRILGWMAVVPCVIFVFAKGRGYYAAPLYPMLIAAGSVQWQHWLDRRSLR
                     MHRVGWAASAALLLCGLVVLRACLPLAAVNSPHWALINELNGDFREEFGWPELTREVA
                     RIWNSLTPAERAEGGIWASNYGEAGALELYGPQYGLPPVISAVNSFWLRGPPPVTPKI
                     LIVVGSRQQDMEKYCTSFAVAGHTGNDAHVANEETERHPDIYICRDPRKSLDAVWPKL
                     LEFG"
     gene            complement(7051..7127)
                     /locus_tag="VFF05_08630"
     tRNA            complement(7051..7127)
                     /locus_tag="VFF05_08630"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7091..7093),aa:Arg,seq:cct)
     gene            7366..7851
                     /locus_tag="VFF05_08635"
     CDS             7366..7851
                     /locus_tag="VFF05_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZW51914.1"
                     /translation="MIVPIANRMLGNLSAILDKGAAFAEQKKIEQTVLTGARLAPDMF
                     PLTRQVQIACDMIKGAAARLGGVEIPSHPDNETTFAELKARVAKVRDFINGIPAANFA
                     GSENRPLTLKMRHGDKHFDNGLDYLRFYAMPNFYFHITTTYTILRHNGVELGKADFIG
                     A"
     gene            complement(8024..>9317)
                     /locus_tag="VFF05_08640"
     CDS             complement(8024..>9317)
                     /locus_tag="VFF05_08640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZW51915.1"
                     /translation="KFPSPYWGPSFRSVIASVSVDGGDPQTLVDAQGSTVFAYAPDSD
                     RFAFQRPRNGDQNNGNAVYVNVNGKISDATAALARNFNGYAWLPGGKALLTFGDLGTN
                     STIWEQPVDGKARTLDLGDVQVSSGLSVSSHGAIAFIGATSTQPAELYVLDSARAKPR
                     RLTHVNAFVDALNIGRSEAMQWQGPDGFAEDGVLTYPPGFDKSKTWPLVLQIHGGPEA
                     ASTMRFSPLTQLLASAGFVVFQPNYRGSTNLGDAYQHAIFRDTGEGPGKDVMAGVDAV
                     KKLGFVDAKRIGVSGWSYGGYMTTWLTGHYDTWKAAVSGAALTDWVMDYTISYYQMGD
                     VYFFGGAPWMEKSREIWREQSPIQYAAKVKAPTLIMGDVGDSNVPLVNSYEWYHALRD
                     NGVDVEFYAYPADSHFPHDIVQSVDVYRRWVGWMQKHL"
BASE COUNT         1598 a         2923 c         2949 g         1847 t
ORIGIN      
        1 gagttcggaa cgtttggccg cgtcgacgag atcggtcgag actccgttct tgagcagatc
       61 ggcaatgact ccatccaggt gcttttgcgc ctgcgccgca tctccgcctt tcggaaatcc
      121 cacttcgacg atgccgatgc cgccgtggcc gaacggctgc acgccggcgt cggaagagag
      181 cactttgccc tgtgcggcca gttccgagag gctgctgcgc gcgttgccca atacgtccat
      241 gagcacttcc gaggccgcgt aatctttcga tttcatgccc ggcatgcggt acatgaattg
      301 caccgagccg gtgccctgcg gcgtggtctt gctgatcgtc gagggtttga acgcggccag
      361 cttcaccgat gcgcgcgcgg gcgtgtcgtg gtgcgggatg gaaccgaaca attccttgac
      421 cttggccagc gtcgcctgcg cgtcgacatc gccggcgatc accagcaggg cgttgttcgg
      481 cacataccag gaatcgtaga agccttgcag gaccttgccg gtggtcttgt cgaacgaagg
      541 gcgcgtgccg agcgcgtcct cggcgtagcc ggtgccggca tagagcgcac gttcggcatc
      601 ggcgaaggcg aggtagcccg gatcggatat gtcgcgcgaa acttcctgct cgatcgcgcc
      661 tttctccagg ccccaatcct tgtcgctcaa ctgcgcaccg cgcatgcgcg tagcttcgat
      721 cttcagcagc aggtcgacgt attgcgcagg cgccacgaag taatactggg tggcatcgtt
      781 ggtggtgaag gcgttgttct cggcgcccat cttgccggtc atctcgttca actgcgaacc
      841 ggacaatccc ttgctgtcgc ggaacatcat gtgctcgagc gcgtgcgcgg ttcccggaaa
      901 accctgtggc gtctcgtaac tgccggccat gtacgtgatc tgcgtcgtca ccatcggtgc
      961 cagcgtgtcg tgcacgatca ccacgcgcag gccgttgtcc agggtcgcgc gcaagacgtt
     1021 cgactgttct gcggatgcaa ccgggccttc ggccagcagc gacggcgcgt agccggttgc
     1081 gagtccgcag cccaacgcga ctgccagcgc gaacgggctt atctttttct tcaaatacat
     1141 ccggttactc ccgtttattg aggcaaaaac taactggatt ccatcaaacg atgcgtggtt
     1201 ctggctcgat acggacgccg aagcgcgcct gcaccgcatc gacgatgcgt tgcgctagcg
     1261 tgcggatctg ttcgccggtc gccgcgccgc gattgaccag caccagcgcg tggcgctcgg
     1321 acacgccggc gtcgccctcg cgaaatccct tgaagccggc ggtttcaacc agccaggcgg
     1381 cggacagctt gcgctgccca tgctcggcgg gccaactcgg cagtgccggg taagtgcgct
     1441 gcagatcggc cgcctgcgat tcgggcacca gcggattctt gaagaaactg ccggcattgc
     1501 cgatttcggc ggggttcggc agtttgcgca ggcgcaggcg catcacggcc tcggccacgg
     1561 ccgcgtgatt cggtgcgtcg gctttcattg ccaccagctc ttcgcgcacg ccggcgtagt
     1621 ccaggcgcag ttcgcgctcg cgcggcagcc ggaattcgac tgcgcaaacg aggtagcgat
     1681 ccggttcgtg cttgaacagc gagtcgcggt aagcgaaagc gcaggcagca ttggccaatc
     1741 gcacgagctg accactgcgc cggtcgaacg cctcaacggt ggcgatgaac tcgcttgcct
     1801 ccacgccgta ggcgccgatg ttctggatcg gtgcggcacc gacactgccc ggaatcagga
     1861 tcaggttttc caggcccgcg aagcaatgtc ccagcgacca gtgtacgaat tcgttccagt
     1921 tctcgccggc ctccacgcgc accgatgcat tatcgccacg atcttcgagg atgcggattc
     1981 cgcgcatgat cggcgagagc accacgcccg gccagtcgcg ggtgagcaac acgttgctgc
     2041 cttcgccgag gatcagcagc ggcgatcgtt gcacggcgcc gaggccgaac acctccggca
     2101 gcgcgcgcgc atcgtccacc tcgatcagcc attgcgcgcg ggcaggcacc cggaaactgt
     2161 tgtggcgcgt gagatccgcg tcctcggtga cgcgaaagcc ggacgcgctc aaggtgtccg
     2221 gtttcatcgt gcggcccgtt tccggatcgc ttcgacgcat tcgccgatca gtgccggacc
     2281 gtgatagacc atgccggaat agaactgcac gagactggca ccggcggcga ttttcgccgc
     2341 cgcgtccgca ccggagagaa tgccgccgac accgatgagc ggaatcttgc cgccgagccg
     2401 ttgtgccatc ccgcgcaata tggaagtcgc tttatcgaac aatggcttgc cggataaacc
     2461 tccagcttct tccgcgtgcc gcgcgcctgc aacggcgctg cgatcgatgg tcgtattggt
     2521 acagatcagg ccatcgattt ttgccgtcag cagcacctgc gcgatgccat ccatttcgcc
     2581 ttcggcaaga tccggtgcga tcttgagcag catgggtttg cgcgtgccat gccggctcgc
     2641 aagtttttcc tgcgcatcgc gcagggtgcc gatgaaacgc ttcagcgttt cctcctcctg
     2701 caaatcgcgt agaccttgcg tgttcggcga ggaaatgttc acggtcacgt aggtggcgca
     2761 ggcatagacg cgctcgaggc agtagcgata atcctcgacg gcatcctcgt tccgtgtgtc
     2821 cttgttcttg ccgatattga tcccgagcac gccagaaaaa cgcgcgcgct cgacgttgtg
     2881 caccagcgcg tcgacaccgg cgttattgaa acccagccgg ttgatcaccg cctgatgttc
     2941 ctgcaaccgc cacatgcgcg gcttcggatt gccgggttgc gcgcgcggcg tggtcgtgcc
     3001 gatttcgatg aagccgaaac ccagggcggc cagtgcatcg atatgcgcgc cgttcttgtc
     3061 gaggccggcg gcgagtccaa ccggattcgg aaattcgatg ccgaacacga ttgtcggcag
     3121 cggcgccggt ttcgccgcca gcaagggatt caggccggta cggtaggcca cctcgatggc
     3181 cgtcaacgcc aggtcgtgcg cgcgttcggc gtcgagacaa aaaagaaacg ggcgggtcag
     3241 cgcgtacaag gacggcgttc aagtcttgga caggcgcggc ggcgcccggc gtttctcggc
     3301 gatttcgacc atcgtttcga tgcggatcat gcgtcggtcg aggtcggtca tcagttgcgt
     3361 caaagtctcg acgttgcgcg cgagacggtc catgtcgccg cgcatctgca gcacatcctt
     3421 gagcacggaa atggcgtcgg ccaagacgcc catgtcagcg cttgcccacg cgacgacgcg
     3481 cgcgcggttc ggccacgcga tgctgtggac tgccgccacg catgacggca atggcgcgct
     3541 cggcggcggt attggcatcg tgcaggctgc ggcgcatgcc ggggatcgct tcgcgcaatt
     3601 cgcgggtcag cgcggcaagt tctcgggttt cgtcgaccga actcgcgcca taggcttgca
     3661 ccgcttggcg cacgagttcg cttgcggtca tgccgcggtc ctcggccagg ccctgcaact
     3721 ggcgcttgtc gtccggtgaa atcagcaagg tgacgcgttc ggttttcatg gcgggctccg
     3781 cagcatgtac attaacatat catgcacatg ctgcgtttcg caacgccaga ggtttctcag
     3841 aaatcgaact tgatgccctg cgccaacggc aacgcgtcgg agtagttgat cgtattggtc
     3901 tggcggcgca tgtacgcacg ccacgcgtcc gaacccgatt cgcgcccgcc gccggtttcc
     3961 ttctcgccgc cgaacgcacc gccgatctcg gcgcccgagg taccgatgtt gacgttggcg
     4021 atgccgcagt cgcttccggc ggcgccgaga aacgcttcgg ctgccttgag gttctgggtg
     4081 aagatcgacg acgacagtcc ctgcggcacc gcattctgct gcgtgatggc ttcgtcgatg
     4141 gtcttgaatt tcatcacgta caaaatcggc gcgaaggttt cggtctgcac gacctcgtcc
     4201 gaatttttca ggcccgtaat gatcgtcggc aacacgaaat tgcctttgcc ttcgatcgcg
     4261 gcgccgccgg tggcgacctt gccgccgctg gccttggcct tctcgatcgc gccgagatag
     4321 gcgttcaccg cgtcgcggct gttgagcggg cccatcaggt ttttcgcgtc ggtcgggtcg
     4381 ccgatctttt tttcgacctg cttgtacgct gccacgagcg tggcgagcac gtcgtcgaag
     4441 atggattcgt gcacgaacag gcggcgcgtg gtcgtgcagc gttggccggc cgtgccgacc
     4501 gcgccgaaaa cgatcgccgg gatggcgagt ttcaggtctg cgcttgcatc gacgatgatc
     4561 gcgttgttgc cgccgagttc cagcagcgag cggcccatgc gctgtgcgac gcgctcgccg
     4621 acgatgcggc cgacgcgcgt ggagccggtg aagctgatca gcgcgatgcg cttgtcgtcg
     4681 acgaactgcg aggcgagttc ggtgccggcg tcgttgaaca ggaagaagat gtccgggaag
     4741 ccgccggact tcaacgcctc gttgcagatg cgcatggcgg caatcgccga cagcggcgtc
     4801 ttcggcgacg gtttccacag cgtgatgtcg ccgcagacgg ccgcaacgaa cgcgttccag
     4861 gcccacaccg cgaccggaaa attgaacgcg ctgatcacgc cgacgaggcc gagcgggtgc
     4921 cactgctcgt acatgcgatg gccggggcgt tcggaatgca tggtcaggcc gtagagctgg
     4981 cgcgataggc cgaccgcgaa gtcgccgatg tcgatcattt cctgcacttc gccgtcgcct
     5041 tccggcttgg acttgcccat ctccagagcg accagcgagc cgagcgcatc cttgtgcttg
     5101 cgcagcgcgt cggcgcacag gcgaatcgct tcgccgcggc gcggcgcggg ggtcgtgcgc
     5161 caggtcgcga aagcttcctg tgcgcgcttg actatcgccg cgtagtcgct cgcgctggag
     5221 gcgtggactt cggcgatgac ttcattggtg ctcggattga tcgattgcaa caggccggca
     5281 tcggctgtct tcgaccactc gccgtggccg atgtaggtgc cggaattatt cgggccgagg
     5341 ccgagggcct tgaggatggg ggtggacatg cagactcctg gaaaacgcca agcgcgcatt
     5401 ataagcgatg cctgcgtgcg ggcgcggcga ttgccgcggc acatgaaggt agaatcggct
     5461 tccctcaccc atccaagatg cctcgatgtc cgacgacaac cccgcagtgc caagcgcgcg
     5521 gcggcagata ttcagcgacc gcgatctatg gattctcatc gcgctggccg ccgccaagct
     5581 ggcttttcac cttgccacga acggacagta cggcttccat cgcgacgaat tgcagacgct
     5641 cagcgactcg catcatctgg attgggggtt cgtcgcctat ccgccgctga cgccgttttt
     5701 cgcccacgtc gagtggctgc tgttcggcaa ctcgctgatc ggtctccgca gtttcgcggc
     5761 cttggcacag agtatcgtct tcgtcgtcac cggcttgatc gcacgccgcc tcggtggtgg
     5821 ccgcgccgca atgttgttgg ccgctttcgc cgtggcgttg tcgccggtgt cgttgtcggc
     5881 cagttcgctg ttgatgtacg tcacgttcga tttcctgtgc ttcgtgttgc tcgcctactt
     5941 cgtggttcga cgcatcgagt cgggagacgc acgctggtgg atcaccatcg gcgcgattgt
     6001 cggcgtgggc atgatgacga aatacacgat ggcgtttttt gccctgggcc tggcggccgg
     6061 agtgttcttg acaccattgc gcgcagatct gcgtggcaag tggccatggc tgggcatggc
     6121 cttggccgtg ctgattttcc tgccgaacct gctgtggctg gcacggcacg atttcatcac
     6181 cctgactttt ctccagttca tccatgcgcg tgacgtgcgc atcggccgca cggatggatt
     6241 ctggatcgat caaatcaaga tcgcggcgaa cttcgccacg gtgccgttct ggctggcagg
     6301 gctctggttt ctgtttttcg ccgaggctgg ccgccgctgg cgcattcttg gctggatggc
     6361 ggtcgtgcca tgcgtgattt tcgtgttcgc caaggggcgc ggttactacg ccgcgccgtt
     6421 gtatccgatg ctgatcgcgg cgggcagcgt gcaatggcag cattggctgg atcggcggtc
     6481 gctgcgaatg caccgtgtcg gctgggcggc atccgcggcg ctgctattgt gcggtctggt
     6541 cgtgctgcgc gcctgcctgc ctttggctgc ggtgaattcg ccgcattggg cgttgatcaa
     6601 cgagttgaac ggggacttcc gcgaggagtt cggctggccc gagctcacgc gcgaggtcgc
     6661 gcgcatctgg aattcgctga cgcccgcgga gcgcgccgag ggcggcatct gggccagcaa
     6721 ttacggcgag gccggcgcgc tcgaacttta cggcccgcaa tatggattgc cgccggtgat
     6781 cagcgcggtc aactcgttct ggctgcgtgg gccgccgccg gtcacgccga aaatcctgat
     6841 cgtggttggt tcgcggcagc aggacatgga aaaatattgc acgtcgttcg cggtcgccgg
     6901 gcacacgggc aacgatgcac acgttgccaa cgaggagacc gagcggcatc ccgacattta
     6961 catttgccgc gacccacgca agtcgctcga tgccgtatgg ccgaagctgc tcgaattcgg
     7021 ctagcgcagt gtgtgcgccc gattccggtg tggcgacccc ggcccgattc gaacgggcga
     7081 cctcgccctt aggaggggcg cgctctatcc agctgagcta cggggccttt ggcgatcatt
     7141 gtagcccgcg ctcggcatcc ttgcggcaac tccgttcggc gtcctgcctc accctcgcgt
     7201 cgagcgcgcg atgccggcgg gcatcgcgca aacgctcgtc gctcgaccag ctgagctacg
     7261 gggccttcgg ttatcatcgt agcgtctgat tcgcgctcta gcggattacc cttttcggac
     7321 ctctccccac aacaaggaat ccccatgcaa gtttcgatgt acgaaatgat cgttccaatc
     7381 gccaaccgca tgctcggcaa cctgtccgcc atcctggaca agggtgcggc cttcgccgag
     7441 cagaaaaaga tcgagcagac cgtgctgacc ggcgcgcgcc tcgcgccgga catgtttccc
     7501 ctgacgcgcc aggtgcagat tgcttgcgac atgatcaagg gcgcggcggc gcgcctgggc
     7561 ggagtggaaa ttccttcgca cccggataac gaaaccacgt tcgccgaact caaggcgcgc
     7621 gtcgccaagg tacgggattt catcaacggc attccggcgg cgaatttcgc cggtagcgag
     7681 aatcgcccgt tgaccctgaa gatgcgccac ggcgacaagc acttcgacaa cggcctggat
     7741 tacctgcgtt tctacgcaat gccaaacttc tacttccaca tcaccacgac gtacacgatt
     7801 cttcgccata acggcgtcga actcggcaag gcggacttca tcggagcttg acgaacaaac
     7861 gggcgcgcca ttcaggcgcg cccaattttt gaattccccg aaaaccgttg cgagcgaaga
     7921 cagatagcgc gccgccggag cgcaggaacc ggagtgtact ttgaacgtac acgaggattc
     7981 cgagcaccgc tggcacgcga gatgtcgagc gcagcaggtt ttctcacagg tgtttctgca
     8041 tccaacccac ccagcgcctg tacacatcca cgctctgcac gatgtcgtgg gggaaatgcg
     8101 agtcggcggg gtaggcgtaa aactccacgt cgacgccgtt gtctctcagg gcgtggtacc
     8161 actcgtagct gttgaccagc ggcacgttgg aatcgccgac gtcgcccatg atcagcgtcg
     8221 gcgccttcac cttagccgcg tactggatcg gcgactgttc gcgccagatc tcgcgggatt
     8281 tctccatcca cggcgcgccg ccgaagaaat acacgtcgcc catctggtaa tacgaaatcg
     8341 tgtagtccat tacccagtcg gtcagcgccg caccggatac agcggccttc cacgtgtcgt
     8401 aatgccccgt cagccaggtc gtcatgtagc cgccgtacga ccagccggaa acgccgatgc
     8461 gcttggcgtc gacgaagccg agtttcttga ccgcgtccac gccggccatc acgtccttgc
     8521 ccggaccctc gccggtgtcg cgaaagattg cgtgctggta ggcgtcgccg aggttcgtgc
     8581 tgccgcgata attgggttgg aaaaccacga aaccggcgga ggcgagcagt tgcgtcagcg
     8641 gcgaaaaacg catcgtcgag gccgcttccg gaccgccgtg gatttgcagt accagcggcc
     8701 aggtcttgga tttgtcgaat ccgggtggat aggtgagcac gccgtcttcg gcgaaaccgt
     8761 caggaccttg ccactgcatc gcttcactgc ggccgatgtt cagcgcatcg acgaaagcgt
     8821 tcacatgagt caggcggcgt ggtttggcgc gcgccgaatc cagcacgtac aactcggccg
     8881 gctgcgtgga agttgccccg ataaaggcaa tcgcgccatg cgacgacaca ctcaagccgg
     8941 aagaaacctg cacatcgccc agatcgagcg tgcgcgcctt gccatcgaca ggctgttccc
     9001 agatcgtcga attcgtgccc agatcaccga aggtcagcag cgccttgccg ccgggcagcc
     9061 aggcataacc gttgaagttg cgtgcgagtg cggcggtggc atcgctgatc tttccgttga
     9121 cgttgacgta caccgcattg ccgttgttct ggtcgccgtt gcgcggacgc tggaaggcga
     9181 aacgatcgct gtctggtgcg tacgcgaaca cggtcgaacc ctgagcatcg accagcgtct
     9241 gcggatcgcc gccatccacg ctcaccgagg cgatcacgct gcggaacgac ggaccccagt
     9301 agggcgaggg aaatttc
//
LOCUS       DASRVO010000694         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_47193, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000694 DASRVO010000000
VERSION     DASRVO010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47193"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VFF05_08645"
     CDS             <1..901
                     /locus_tag="VFF05_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZW51916.1"
                     /translation="GFSEGLGLTRPTMYFGAFGLVLLAPIGYVFMYGAFGLPARGAVG
                     SGMATATVLWIQFAVFALYIARRRHYTIYAPFAHWQWPQPHAIGELLRLGVPMGISLF
                     MEGGLFVTAALLIGSLGADVVAGHQIAINVASVTFMVPLGLAMATTVRVGRAAGGGDM
                     VGARWAGFAGIGASLASQVVSCALMALFPHAIAALYTDDAAVVAIAAQLLLFAAAFQL
                     SDGIQVTANGALRGLKDTNGPMAITTLAYWGIGMPAGYLLCFHFGHGAPGMWTGLIAG
                     LSVAAVLLFVRFAREARGGIAQA"
     gene            965..>2144
                     /locus_tag="VFF05_08650"
     CDS             965..>2144
                     /locus_tag="VFF05_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZW51917.1"
                     /translation="MAAQQPNIIVRFFAGIWALFNFTRRLVFGVIALILLIGFIAALR
                     TAAPKLLDKTALVLDPKGAIVEQYTNDAAQRALSNLAGDKSKQTQLRDILTAIDAAAK
                     DPRIARIVLEPDGIDSAGLAISREIGAALDRFKATGKPVIAVSNGMTQGQYYLAAHAS
                     TILLHPASLEGVLLTGFGAYRSYYKDALDWLGVDVHVFKVGTFKSYPEPYVRNDASPD
                     AKEADLFWMNDLWGDYLKDIAAARHLDVAKISAQIADYADAVKSAGGDMAKLALADKL
                     VDKLATPDEARQMLIADGARDRHSYRQIDFADYAALVEREHALDTRPQVAVVVAEGEI
                     LPGDQPQGTVGGKSTAKLLRAARNDDAVKAVVLRVNSPGGDAFSSELIRREVELTKAA
                     G"
BASE COUNT          331 a          701 c          723 g          389 t
ORIGIN      
        1 cggattttcc gaaggcttgg gcctgacccg tccgaccatg tacttcggcg cgttcgggct
       61 cgttctgctc gcgccgatcg gctacgtgtt catgtacggc gcgttcggcc tgcctgcgcg
      121 cggcgccgtg ggttcgggca tggccacggc cacggtgttg tggattcagt tcgccgtgtt
      181 cgcactctat attgcgcggc gccgtcatta cacgatctac gcgccgttcg cgcactggca
      241 gtggccgcag ccgcatgcga tcggggaatt gctgcgtctg ggcgttccga tgggcatctc
      301 gttgttcatg gaaggcggcc tgtttgtcac cgcggcgctg ctcatcggct cgctgggcgc
      361 ggacgtggtc gcgggccatc agatcgcaat caacgtcgcc tcggtcacgt tcatggtgcc
      421 gctgggcctg gccatggcga cgaccgtgcg cgtgggccgc gcggcgggag gcggcgacat
      481 ggttggcgcg cgctgggcgg gcttcgccgg catcggcgcg tcgctggcat cgcaagtcgt
      541 gtcgtgtgcg ttgatggcgt tgtttccgca tgcgatcgcg gcgttgtaca ccgacgatgc
      601 ggccgtggtc gcgatcgcgg cgcaattgct gctgttcgcg gcggcgttcc agctctccga
      661 cggcatccag gtcaccgcga acggcgcact gcgcgggctc aaggacacga acgggccgat
      721 ggcaatcacc acgctcgcct actggggcat cggcatgccg gcgggctacc tgctgtgttt
      781 ccacttcggc cacggcgcgc cgggcatgtg gaccggcctc atcgcgggcc tgagcgtggc
      841 cgcggtgttg ctgttcgtgc gcttcgcgcg cgaagcgcgc ggtggcatcg cgcaagcatg
      901 accttcagcg cgtgatcgcg gcccgcgcat cggttaggct tgccgccttc ttgtggagaa
      961 tttcatggct gctcagcaac ccaacatcat cgtgcgcttt ttcgccggca tatgggcctt
     1021 gttcaatttc acccggcgcc tggtgttcgg cgtcatcgcg ctcatcctgt tgatcggctt
     1081 catcgccgcg ttgcgcacgg ctgcgccgaa gctgctcgac aagaccgcac tggtgctcga
     1141 tccgaaggga gccatcgtcg agcagtacac gaacgatgcc gcccagcgcg cgttgtcgaa
     1201 tctcgccggc gacaagtcca aacaaacgca gttgcgcgac atcctcaccg ccatcgacgc
     1261 ggcggccaag gatccgcgca tcgcgcgcat cgtgctcgag ccggacggca tcgacagcgc
     1321 cggcctggcg atatcgcgcg agatcggcgc cgcgctggat cgcttcaagg cgaccggcaa
     1381 gccggtgatc gcggtttcca acggcatgac ccagggccag tattacctcg ccgcgcacgc
     1441 gagcacgatc ttgctgcatc cggcatcgct cgaaggcgtg ctgctgaccg gcttcggcgc
     1501 ctaccgcagc tactacaagg acgcgctcga ctggcttggc gtggacgtgc acgtgttcaa
     1561 ggtcggcacg ttcaagtcgt acccggaacc ctacgtgcgc aacgacgcct cgcccgacgc
     1621 aaaggaagcg gacctgttct ggatgaacga tttgtggggc gattacctga aggacatcgc
     1681 cgccgcacgg catctggacg tcgcgaaaat ttccgcgcag atcgccgatt acgccgatgc
     1741 ggtgaagtcc gcgggcggcg acatggccaa actcgcgctc gccgacaagc tcgtcgacaa
     1801 gctcgccacg ccggacgagg cgcggcagat gctgatcgcc gacggtgcgc gtgaccggca
     1861 cagctaccgc cagatcgact tcgccgacta cgcggcgctg gtcgagcgcg aacatgcgct
     1921 ggacacgcgc ccgcaagtcg cggtcgtcgt ggccgaaggc gaaatcctgc ccggcgacca
     1981 gccgcaaggc acggtcggtg gcaaatccac ggcgaaactg ctccgcgccg cgcgcaacga
     2041 cgatgcggtc aaggcggtcg tactgcgcgt gaattcgccg ggtggcgacg cgttctcgtc
     2101 cgaactgatc cggcgcgaag tggaattgac gaaagcggcg ggca
//
LOCUS       DASRVO010000695         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_149400, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000695 DASRVO010000000
VERSION     DASRVO010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149400"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFF05_08655"
     CDS             <1..733
                     /locus_tag="VFF05_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZW51918.1"
                     /translation="ALAPLLCEHFHIVAIDFPGHGRSDWRPPGAWYHYVDYLSDALAT
                     ADALRWERFGLLGHSLGGAVASMLAGACPQRVERLLLIEALGPPTQAPEQTLVQLQRA
                     FAERDAAAAKTPRVFASVDEAVSARLRANDLSREAATLLVERGVKTVAGGLTWSTDPR
                     LLLTSPVRNTEEQVLAVLRGIRAPTALILAEPEQPYMPRTTMDRRIAQVRGIQVRRLA
                     GTHHLHLEDAPTVAKTILDFERIVP"
     gene            863..1582
                     /locus_tag="VFF05_08660"
     CDS             863..1582
                     /locus_tag="VFF05_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZW51919.1"
                     /translation="MMQPQRQTVFVAHGLTKVYRMGDVEVRALNGVDLEIHAGEFIVL
                     LGPSGSGKSTLLNILGGLDTPTAGTAFWRDHDLARADDAELTRYRRDHVGFVFQFYNL
                     IPSLTVRENVALVTDIAPNPMRPEEALALVGLGDRLDHFPAQLSGGEQQRVAIARAIV
                     KRPEVLLCDEPTGALDYATGKRVLDVIARVNSELGTTAIVITHNAAIAGMADRVLYLA
                     DGRIARMQANPRRLTSEEVRW"
     gene            1579..>2488
                     /locus_tag="VFF05_08665"
     CDS             1579..>2488
                     /locus_tag="VFF05_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW51920.1"
                     /translation="MRAIDRKLLRDLAQMRGQVLTIALVVACAVAGFVGTLSTYASLD
                     LARQTFYEQARFGHVFSSLKRAPNDLATRLAQIAGVADVQTTVVFDATLDLPGVQEPL
                     VGRMVGVPAGGVRRLNRIVLRRGRMLDPVQPHEAIVSEAFAAARGLGPGSEVGVLLNG
                     RRETLKIVGVGLSPDYVYATQGGAFPDDRNFGVLWMDRGYLASAYDMEGAFNDVVVRL
                     APHASERAVIDALDRRLEAYGGANAYGRDEQISNRILDEEINQWKVIASILPTIFLGV
                     AAFLLNVVLSRQINAQREQIAALKALG"
BASE COUNT          403 a          844 c          819 g          422 t
ORIGIN      
        1 cgcgctcgcg ccgctgcttt gtgaacattt ccacattgtg gcaatcgatt ttcccggcca
       61 cggacgctcc gactggcgtc cacccggcgc ttggtatcac tatgtcgatt acctgagcga
      121 cgcgctcgct acggcggatg cgctgcgctg ggaacgcttc ggcctgctcg gtcattcgct
      181 cggcggtgcc gtggccagca tgctggccgg cgcgtgcccg caacgtgtcg aacgtctgct
      241 gctgatcgag gccctgggtc cgcccacgca ggcgccagag cagacgttgg tgcagctgca
      301 gcgcgccttt gccgaacgcg acgcggcggc tgcgaagacg ccacgcgtgt ttgcaagcgt
      361 cgacgaggcg gtgtcggcac gtctgcgcgc caacgacttg tcgcgcgaag cggccacact
      421 tctggtcgaa cgtggcgtca agacagtcgc aggcggcctg acttggtcca ccgatccgcg
      481 cctgttgctc acctcgccgg tgcgcaacac cgaagagcag gttctcgccg tgctgcgcgg
      541 cattcgcgca ccgaccgcgc tgattctggc cgaacccgag cagccctaca tgccgcgcac
      601 gacaatggat cggcgcatcg cgcaggtacg gggcatccag gttcggcgct tggctggaac
      661 gcaccatctg catctggaag acgcgcccac cgtggccaag acgatcctgg atttcgagcg
      721 aatcgtgcct tgaatgcagg aggcgaacca gcgcgcgtcg cgcgggaacc gagtgccgcg
      781 cccgaggtcc gggcaatttg accttcgtca accggacgca gcgccattgc gagacactga
      841 ccaggcatat ccgggactcg cgatgatgca gccgcagcgg cagacggttt tcgtggcaca
      901 cgggcttacc aaggtctatc gcatgggcga tgtcgaggtg cgcgcgctga acggcgtcga
      961 cctggaaatc catgccggcg aattcatcgt gctactcggt ccctcgggca gcggcaagtc
     1021 caccttgctc aacatcctcg gcggcctgga cacgcccacc gctggcacgg cgttctggcg
     1081 cgatcacgac ctggcccgtg cggacgacgc cgaactcacg cgttatcgcc gcgatcacgt
     1141 cggtttcgtg ttccagttct acaacctgat ccccagcctc acggtccgtg agaatgtcgc
     1201 cctcgtcacc gacatcgccc cgaatccgat gcgccccgag gaggcgctcg cgctggtggg
     1261 ccttggcgac cggctcgatc acttcccggc gcagctgtcc ggcggcgagc agcaacgcgt
     1321 ggcgatcgcg cgggcgatcg tgaaacgccc ggaggtactg ctctgcgacg agccgaccgg
     1381 cgcgctcgac tacgcgaccg gtaagcgcgt gctcgatgtg atcgcgcgcg tcaacagcga
     1441 acttggcacc accgccatcg tcatcacgca caacgccgcg atcgccggga tggccgaccg
     1501 cgtgctgtac ttggccgacg gccgcatcgc gcgcatgcag gcaaacccgc gccggctcac
     1561 gtcggaggaa gtgcgctggt gagggcgatc gatcgcaagc tgctgcgcga cctggcgcaa
     1621 atgcgcggcc aggtactgac gatcgcgctg gtcgtggcgt gcgcggtcgc gggcttcgtc
     1681 ggcacgcttt cgacatacgc gtcgctcgat ctcgcgcgcc agacgttcta tgaacaggcg
     1741 cgtttcggcc acgtcttcag ttccctcaaa cgcgcgccga atgatctcgc cacgcgcctg
     1801 gcgcagattg ccggcgtcgc cgacgtacag accaccgtcg tgttcgacgc gaccctggat
     1861 cttcccggcg tgcaggagcc gctggtcggt cgcatggtcg gcgtgcccgc gggtggcgtg
     1921 cgccgcttga accggatcgt cttgcgccgc gggcgcatgc tcgatccggt gcaaccgcac
     1981 gaggcgatcg tttcggaggc cttcgcggcg gcgcgcggac tagggccagg cagcgaggtc
     2041 ggcgtgctgc ttaacgggcg gcgcgaaacc ctgaagatcg tgggcgtagg cctgtctccc
     2101 gattacgtgt acgccacgca aggcggcgcg tttccggacg accgcaattt cggcgtgctc
     2161 tggatggatc gcgggtacct ggcctcggct tacgacatgg aaggcgcgtt caacgacgtg
     2221 gtggtccggc tggcaccgca cgcgagcgaa cgcgccgtca tcgacgccct ggatcgccgg
     2281 ctcgaggcct acggcggcgc caacgcctac ggccgtgacg agcaaatctc caaccgcatc
     2341 ctcgacgagg aaatcaacca gtggaaggtc atcgcctcaa tcctgccgac gatcttcctg
     2401 ggcgtggccg cattcctgct caacgtcgtg ctgtcgcggc agatcaacgc gcaacgcgaa
     2461 cagatcgcgg ccctgaaggc gctgggtt
//
LOCUS       DASRVO010000696         1391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_174959, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000696 DASRVO010000000
VERSION     DASRVO010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174959"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VFF05_08670"
     CDS             complement(<1..1300)
                     /locus_tag="VFF05_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HZW51921.1"
                     /translation="MLENLERAGYTGDVHLINPKRAEINGRPCKPSIDELPMGVDCAV
                     LAIPHAAVLESVRACARRGVGGVIIFSAGFAEGGEEGRVEQAEITRIAREHGMIVEGP
                     NCLGLVNYIDGVPLTFVQTQVRRTGGKPGIAVVSQSGAMAAVLGVSLAAHDLTVSFSV
                     STGNEAVSGVEDYLEYMLQDSSVRVITMIVEQFRQPKRFLDLARRARQIGKAIVLLHP
                     GRSSAARESAATHTGAMAGDYQVMRSLVARTGVIIVDALEELLDVSDIALRCASRPQG
                     GCGVLTESGAFKALTLDLCEQVGLELSPLNDAVRSGLRAVLPDFIPASNPMDLTAQAL
                     VDPDLYRHTLAVLLGDESFGSVVLGLILTDKATCDLKLPSVIGAIKELKPAKPVIFAA
                     LDDGADVPKAYIEELRALGIPCFPSAERAFRAVAHLSAAVP"
BASE COUNT          266 a          455 c          450 g          220 t
ORIGIN      
        1 ggggcacggc cgcagagaga tgcgcaacgg cgcggaatgc acgctccgcg gacgggaagc
       61 acggaatgcc gagtgcccgc agttcttcga tgtatgcctt cggcacatcg gcaccgtcgt
      121 cgagtgccgc aaaaatcacg ggcttcgcag gcttgagttc cttgatcgcg ccgatcaccg
      181 acggcagttt caggtcgcag gtcgccttgt ccgtgaggat caagccgagc accacgctgc
      241 cgaaggactc atcgcccaac agcaccgcca gtgtgtgccg gtaaaggtca gggtcgacca
      301 gcgcttgcgc agtcaggtcc atcgggttgc tggctggaat aaagtcgggc agtacggcac
      361 gcagtccgga ccgcaccgcg tcgttgagcg gcgagagctc caggccgacc tgctcgcaca
      421 ggtcgagcgt caacgccttg aacgcgccgg attcggtcag cacaccgcaa ccgccctgcg
      481 gccgcgaggc acagcgcaac gcgatgtccg atacgtcaag caactcttcc agcgcatcga
      541 cgatgatgac gccggtgcgt gccaccagcg aacgcatgac ctggtagtcg ccggccatcg
      601 cgccggtgtg ggtcgccgcc gactcgcgcg cggcgctgct gcggccggga tgaagcagga
      661 caatggcctt gccgatctgg cgcgcacggc gggccagatc gaggaagcgc ttcggttgcc
      721 ggaactgttc gacgatcatg gtgatgacgc gcaccgacga atcttggagc atgtactcga
      781 gataatcttc cacgccgctc accgcctcgt ttccggtcga gaccgagaag gaaacggtca
      841 ggtcgtgcgc ggcaagactg acgccgagta ccgccgccat ggcgccgctt tgcgaaacca
      901 cggcgatgcc gggtttgccc ccggtgcggc gcacctgcgt ctgcacgaaa gtgagcggca
      961 cgccgtcgat atagttcacc agaccgaggc aatttggtcc ctctacgatc atgccgtgct
     1021 cgcgggcaat acgggtgatt tccgcctgct cgaccctccc ctcctcgcca ccttcggcaa
     1081 atccggccga gaagatgatc acgccgccga cgccgcgccg cgcacaggca cgcacagatt
     1141 ccagcactgc cgcgtgcgga atcgccagca cggcgcaatc cacgcccatc ggcaattcat
     1201 cgatgcttgg cttgcacgga cgcccgttga tctcggcgcg tttcggattg ataagatgga
     1261 cgtcgccggt atagccggca cgctcgaggt tctccagcac tgcagcacca agggcgccgg
     1321 gtgtaggcga agcaccgacg atggccacgg agcgcggacg cagcagacgg acgatggcgg
     1381 gttggggcaa a
//
LOCUS       DASRVO010000697         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_20487, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000697 DASRVO010000000
VERSION     DASRVO010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20487"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /gene="recJ"
                     /locus_tag="VFF05_08675"
     CDS             <1..1694
                     /gene="recJ"
                     /locus_tag="VFF05_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZW51922.1"
                     /translation="DWPADIHPVLRRVYAARGVVTPADIEHRLAGLLSPQSLGGMARA
                     CELIEAAIREDARIVVVGDFDCDGATGTAVGVRGLRLLGAREVGYAVPNRMRHGYGLS
                     PAIVEELLPRKPGVLITVDNGVSAHAGVAAAKALGMGVIVTDHHLPGATLPDADAIVN
                     PNLDADAFPSKALAGVGVMFYLLLALRARLRELGWFAERAIAEPDLSELLDLVALGTV
                     ADLVPLDRNNRILVEAGLRRIRAGRACAGIAALLAAGKRDPARAVASDLGFAVGPRLN
                     AAGRLEDMTLGIECLLTDDFGRAHELAERLSAINAERRELQDEMTAQAQAEVEKWNSS
                     CGANVLPHGIVLFDPDWHHGVVGLVASKLKETLHRPVIACAPAGEHGDEIKASGRSIP
                     GFHLRDALAEVDACHPGLIQRFGGHAMAAGLSLNRADLEAFGAAFDAVARKHLQAELL
                     DAIVLTDGELDAQDFSLELAQQLRYAGPWGQAFPEPEFEGEFAVESWKVVAEKHLKFK
                     LRQSALGGPLDAIAFNGYSGAPPPARLRAVFQLDVNEWNGQQSLQLLVRHLQACS"
     gene            1797..2870
                     /gene="prfB"
                     /locus_tag="VFF05_08680"
     CDS             1797..2870
                     /gene="prfB"
                     /locus_tag="VFF05_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZW51923.1"
                     /translation="MRRSGGIFDYDSKRERLEEVSRELENPNVWDNSERAQQLGRERA
                     QLDKIVNGIRTLTDGLSGAQDLLELAVAESDEGTAKAVSDDVDGLAAKVETLEFQRMF
                     SGKMDSHNAFVDVQAGAGGTEAQDWSEILLRMYLRWAEARGWKTELLEVSAGEVAGIK
                     SATFRVEGDYAYGWLKTEIGVHRLVRKSPFDSDNRRHTSFASIFVSPEVDDDIDIEIN
                     PADLKTDVYRSSGAGGQHVNKTESAVRITHVPSGIVVACQTERSQHANRDRAMKMLKA
                     KLYEMEVQKRNAEKDALEATKSDIGWGSQIRNYVLDQSRIKDLRTGIERTDTQKVLDG
                     DLDGFIEASLKAGLEAGAKRIDA"
     gene            2884..>4300
                     /gene="lysS"
                     /locus_tag="VFF05_08685"
     CDS             2884..>4300
                     /gene="lysS"
                     /locus_tag="VFF05_08685"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZW51924.1"
                     /translation="MTDDNQPQDENKLIAERRDKLRALRGQGIAFPNDFHPDAFAGDL
                     QGEFDGKGAEEVEASARHVKIAGRMVLKRGQGKVSFVQIQDMTGRIQLFVQANAVGDT
                     YEAFKSWDVGDIVGAEGALMRTKTGELSVKVEKLRLLTKALRPLPDKWHGLADVEQRY
                     RQRYVDLIVTPEARRTFALRSRAIGFMRQWLEAKPRRFMEVETPMMHVIPGGAVARPF
                     VTHHNALDIDLYLRVAPELYLKRLVVGGFERVYEINRNFRNEGVSTRHNPEFTMLELY
                     QAYATYNEVMDLTESMIRETARATTGTTALTWEGRDIDVGSAFRRWSMVDAVLEHNPQ
                     IARADVRDRAKIAAHAEKLGVHVKTGYGWGKIALEIFEKTVEATLIQPTFITMHPTEV
                     SPLARANDADPEITDRFELFIGGKEIANGFSELNDPEDQAARFRAQVASKESGDDEAM
                     HFDADYIRALEVGMPPTGGLGV"
BASE COUNT          829 a         1348 c         1391 g          732 t
ORIGIN      
        1 ccgattggcc tgcggacatc catccagtcc tgcgccgggt ctatgcggca cgcggagttg
       61 tgacgccggc ggacatcgaa caccggctcg ccggtctgct gtcgccgcag tcgctcggcg
      121 gcatggcgcg cgcctgcgag ctgatcgaag ccgccatccg cgaggacgcg cgcattgtcg
      181 tggtcggcga tttcgattgc gacggcgcga ccggcacggc cgtcggcgtg cgcggcctgc
      241 gcctgctcgg cgcgcgcgaa gtcggctacg ccgtacccaa ccgcatgcgc cacggctacg
      301 gcttgagtcc ggccatcgtc gaagagttgt tgccgcgcaa gcccggcgta ttgatcacgg
      361 tcgacaacgg cgtttccgcg cacgccggcg ttgctgccgc caaggcgctc ggaatgggcg
      421 tgattgtcac cgatcaccac ttgcccggcg caacgctgcc ggatgccgat gccatcgtaa
      481 atccgaatct cgacgccgac gcttttccga gcaaggctct agccggcgtc ggtgtgatgt
      541 tttatctgct gctggcattg cgcgcgcgat tgcgcgaact cggctggttc gcggagcgag
      601 cgatcgccga accggatttg tccgaactgc tcgatctggt tgcgctcggc actgtcgccg
      661 acttggtgcc cctggatcgc aacaaccgca tcctcgtcga agcgggactg cggcgcattc
      721 gcgcgggccg tgcctgcgcc ggtattgccg cgttgttggc tgccggcaag cgcgatccgg
      781 cgcgcgcggt agcgagcgat ctcggttttg cggtcgggcc acgcctcaat gcggcgggtc
      841 ggctcgagga catgacgctc ggcatcgaat gcctgctgac ggacgacttc ggtcgtgccc
      901 acgagttggc cgaacggctg tccgcgatca acgccgaacg ccgcgaattg caagatgaga
      961 tgaccgcgca ggcgcaggcg gaagtggaaa agtggaattc ctcgtgcggt gccaatgtgc
     1021 tgccgcacgg gatagtgctg ttcgatccgg actggcatca cggcgtggtc ggtctcgttg
     1081 cctccaagct gaaagagacg ctgcatcggc cagtaattgc ctgcgcgccg gctggcgaac
     1141 acggcgacga aataaaggca tccggtcgat ccattccagg attccacctg cgtgatgcgc
     1201 tggccgaagt cgatgcgtgc catcccgggc tgatccagcg cttcggcggg cacgccatgg
     1261 cggcgggcct gagcttgaac cgtgccgacc tggaagcctt cggcgccgcg ttcgatgcgg
     1321 tcgcacgcaa gcatctgcaa gcggagctgc tcgatgcgat cgtgctcacc gacggcgagc
     1381 tcgatgctca ggatttcagc ctcgaactcg cgcagcagtt gcgttacgcc gggccgtggg
     1441 gacaggcatt tcccgaaccg gaattcgaag gcgagtttgc ggtggaatcg tggaaggtgg
     1501 tggctgagaa gcatctcaag ttcaagttgc ggcagtccgc gctcggcggc ccgctagacg
     1561 ccatagcctt caacggttac tccggtgcac cgccgccggc gcgactgcgc gcggtgttcc
     1621 agttggacgt caacgaatgg aacggccagc agtcgttgca attgctggtc cggcacctgc
     1681 aggcctgcag ctgaggcgcg gttttttgct accatgcgaa atccttttgc acagccccgg
     1741 cgccatgatc gaaaccaacc cgattgccta ccgcattgcc gaccttgcga gccgccttgc
     1801 gtcgctcagg gggtatcttt gactacgatt cgaaacgcga acgcctcgaa gaagtaagcc
     1861 gcgagctcga aaacccgaac gtctgggaca actccgagcg cgcccagcaa ctgggtcgtg
     1921 aacgcgccca gctcgacaag atcgtcaacg gcatccgcac cctcaccgat ggactgagtg
     1981 gcgcgcagga tctgctggaa ttggccgtcg ccgaaagcga cgaaggcacc gcgaaggccg
     2041 tatccgacga cgtggatggc cttgccgcca aggtcgagac gctggaattc cagcgcatgt
     2101 tttccggcaa gatggattcg cacaacgcct tcgtcgacgt gcaggccggc gccggcggca
     2161 ccgaggctca ggactggtcg gaaatcctgc tgcgcatgta cctgcgctgg gccgaggcgc
     2221 gcggctggaa aaccgaattg ctggaggtct cggccggcga agtcgccggc atcaagagcg
     2281 cgacgttccg cgtcgagggc gactacgcct acggctggct caagaccgag atcggcgtgc
     2341 atcgcctggt gcgcaaatcg ccgttcgatt cggacaatcg ccggcacacc tcgttcgcgt
     2401 cgattttcgt gtcgcccgaa gtcgacgacg acatcgacat cgagatcaat ccggccgacc
     2461 tgaaaacgga cgtctatcgt tcatccggcg ccggcggcca gcacgtcaac aagaccgaat
     2521 cggcagtgcg catcacgcat gtgccgagcg gcatcgtggt cgcctgccag accgaacgca
     2581 gccagcatgc caaccgcgac cgcgcgatga agatgctcaa ggccaagctg tacgagatgg
     2641 aagtgcagaa gcgcaatgcc gagaaggatg cgctcgaagc aacgaaatcg gatatcggct
     2701 ggggcagcca gatccgcaac tacgtgctgg atcagtcgag gatcaaggat ttgcgcaccg
     2761 gcatcgagcg caccgatacg cagaaagtgc tcgatggcga cctggatgga ttcatcgagg
     2821 ccagcttgaa ggcagggctg gaagccggcg caaaacgcat cgacgcatga tttggaaggc
     2881 gacatgactg acgacaatca gccgcaggac gaaaacaaac tcatcgccga gcgccgcgac
     2941 aagctgcgtg cgttacgcgg gcagggcatt gccttcccga acgattttca tccggatgcg
     3001 ttcgccggcg atttgcaagg tgaattcgac ggcaagggcg ccgaggaagt cgaagcctcc
     3061 gcgcgccatg tgaagatcgc cggccgcatg gtgctcaagc gcggccaggg caaggtcagc
     3121 ttcgtgcaga tccaggacat gaccgggcgc atccagttgt tcgttcaggc caacgccgtc
     3181 ggcgacacct acgaagcatt caagtcctgg gacgtcggcg acatcgtcgg cgcggaaggc
     3241 gcgctgatgc gtacgaagac cggcgaactt tcggtaaaag tggaaaaact gcgcctgctc
     3301 accaaggcgt tgcgcccgtt gccggacaag tggcacggcc ttgccgacgt cgagcaacgt
     3361 tatcggcagc gttacgtgga tctgatcgtc acgccggagg cgcggcgtac gttcgccctg
     3421 cgctcgcgcg cgatcggttt catgcgccag tggctggaag ccaaaccgcg ccgcttcatg
     3481 gaagtggaaa cgccgatgat gcacgtcatc cccggcggtg ccgtcgcgcg gccattcgtg
     3541 acgcaccaca atgcactgga tatcgacctg tacctgcgcg tcgcaccgga gctttatctc
     3601 aagcgcctcg tcgtcggcgg tttcgagcgc gtgtacgaga tcaaccgcaa cttccgcaac
     3661 gagggcgttt ccacgcggca caatcccgag ttcaccatgc tcgagttgta ccaggcctac
     3721 gccacctaca acgaggtcat ggacctgacc gaaagcatga tccgcgagac cgcacgcgcc
     3781 accacaggaa ccaccgcgtt gacctgggaa gggcgcgaca tcgatgtcgg atccgcgttt
     3841 cggcgttggt cgatggtgga cgccgtgctc gagcacaatc cgcagatcgc gcgggcagac
     3901 gtgcgcgacc gcgcgaagat cgccgcgcat gccgaaaagc ttggcgtcca cgtcaagacc
     3961 ggatatggct ggggcaagat cgcgctggag attttcgaaa agacggtcga agcgacgctg
     4021 atccagccga ccttcatcac catgcacccg accgaagtct cgccgctggc gcgcgcgaac
     4081 gatgccgatc cggaaatcac cgaccgcttc gaactgttca tcggcggcaa ggaaatcgcg
     4141 aacggatttt ccgagttgaa cgatccggaa gaccaggccg cacgcttccg tgcgcaggtg
     4201 gcgtccaagg agtccggaga cgacgaggcc atgcacttcg acgccgacta catccgcgcg
     4261 ctggaagtcg gcatgccgcc gactggcggc ctcggagtcg
//
LOCUS       DASRVO010000698         1264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_280938, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000698 DASRVO010000000
VERSION     DASRVO010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280938"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFF05_08690"
     CDS             <1..193
                     /locus_tag="VFF05_08690"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003295955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZW51925.1"
                     /translation="SAMVPQLVGGLRSGMGLTGSRNIEELRTKSQFVRITAAGLRESH
                     AHDVTITKEAPNYRLEPAE"
     gene            complement(884..>1264)
                     /locus_tag="VFF05_08695"
     CDS             complement(884..>1264)
                     /locus_tag="VFF05_08695"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZW51926.1"
                     /translation="LTGRSEGFIAGRPDLAETIRRLTAYAEAGADCLYAPGLRNIDDI
                     RSVVAAVAPKPVNVLAGSDFTTVAEMARVGVRRISVGGALARVAWAAFLAAAREIAEK
                     GTFAELGKAIPGVEINGAFEVNDA"
BASE COUNT          204 a          434 c          398 g          228 t
ORIGIN      
        1 ctcggcgatg gttccgcagc tcgtcggtgg actgcggtcc gggatgggcc tgaccggctc
       61 gcgcaatatc gaagagctgc gcacgaaatc gcagttcgtg cgcatcacgg cggcggggct
      121 gcgcgagtcg catgcgcacg acgtgaccat caccaaggaa gcgccgaact atcgcctcga
      181 gccggcggag tagcgcggat caggcgacat ccgctcggtc acggtttcgc ttccaacgcg
      241 aaaatcgcct tggggatcgt catgtgcacc cctgattgcc ctcgttctcg gaccaggcgc
      301 ggtgttcgat gagctgagag cagtcatcgt gcttttcgca ttgttctctt cctctgcagc
      361 atttggcgcg atcatcggta caccgccctc cccgagttcg accgatagga tccccatccg
      421 actggaaaac acattcgggg cttaggcgca cgctacgtcc gcgtcgatca ggcaggttgg
      481 caacaccttc gtcattcagc agaacgttga cgctgcttgc caactaccga gcacccccct
      541 ggtcgcatcg gagttccaga tcggaccgct cacgggcggc gcgtatcgcc aacatcaact
      601 ttgcgagtgc ggcctgcttc tttccgccgc ttacgcagac ggcggagttc acggttgcgc
      661 cgccatcgcc gccatccccg tgctccatgg cgcgcgctgc tccttctcgc cggggtactc
      721 gtgctgactg cgctcgcggt gctcagggtg caacggtaga cgaagccgca ccggcactgg
      781 tcgtgcgtat cacggcggcg gggctgcgcg agtcgcacgc gcaggacgtg accatcacca
      841 gggaagcgcc gaacgatcgc ttcgagccgg cgagtcgcgc ggatcaggcg tcattcactt
      901 cgaatgcgcc gttgatctcg acgccgggaa tggccttgcc cagttccgca aacgttccct
      961 tctccgcgat ctctcgtgcg gcagcgagaa acgcggccca cgccacgcgt gccagcgcgc
     1021 cgccgacgct gatccggcgc acccctacgc gcgccatttc cgccaccgtc gtgaagtcgc
     1081 tgccggcgag aacgttgacc ggcttcggcg cgacggccgc gacgacgctg cggatgtcgt
     1141 cgatgttccg aagcccgggt gcatagaggc aatcggcgcc cgcttccgcg taggcggtca
     1201 gccgccggat cgtctccgcc agatccggcc ggccggcgat gaatccctcc gagcggccgg
     1261 tgag
//
LOCUS       DASRVO010000699         1001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_383868, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000699 DASRVO010000000
VERSION     DASRVO010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383868"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VFF05_08700"
     CDS             <1..962
                     /locus_tag="VFF05_08700"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZW51927.1"
                     /translation="PPAQEMRDALTGKLITRIPLGAEFVARFGQPYAVTHRADIHGVL
                     LNACHGNDLITMETDRRVDGFKEQGNGVVVTLESGEEIKGRALIGCDGMWSGIRESIV
                     GDDKPRVSGHIAYRGVLKRADVPADLWRPDVVLWAGPRTHFVHYPLRRGELYNLVAVF
                     HSDRYEEGWNAEGAKDVMWQHFKTQVPQVTRMLERIETWRMWVLCDREPVKEWSKGRV
                     TLLGDAAHPMLQYLAQGACMAAEDAVVLAEKAAASPDNLPAAFKAYEQNRYLRTGRVQ
                     IMARVYGEFYHARGVAAELRDMALAGRTPEQSYDGIAWLYGGP"
BASE COUNT          189 a          315 c          333 g          164 t
ORIGIN      
        1 agccgccggc gcaggaaatg cgcgatgcat tgaccggcaa gctcatcacc cgcattccgc
       61 tcggtgccga attcgttgcg cgtttcggcc agccctatgc cgtcacccat cgcgccgaca
      121 tccacggcgt cctgctgaac gcttgccacg gcaacgacct gatcaccatg gaaaccgacc
      181 ggcgggtcga cggctttaag gaacagggca acggcgtcgt cgtcacgctc gaaagcggcg
      241 aagagatcaa gggccgcgca ttgatcggct gcgacggcat gtggtcagga attcgtgaaa
      301 gcattgtcgg cgacgacaag ccgcgggtgt ccggccatat cgcctatcgc ggcgtgctca
      361 agcgcgccga cgtgccggcc gatctgtggc ggccggatgt ggtgctgtgg gccggcccgc
      421 gcacccattt cgtgcactat ccgctgcgcc gcggcgaact ctacaatctg gtcgccgtgt
      481 ttcattccga ccgatacgaa gaaggctgga acgcggaagg cgccaaggac gtgatgtggc
      541 agcacttcaa gacccaggtg ccgcaggtca cgcgcatgct cgagcgcatc gagacctggc
      601 gcatgtgggt gctgtgcgac cgcgagccgg tgaaggaatg gtcgaaaggc cgcgtcacgc
      661 tgcttggcga tgccgcccat ccgatgctgc aatacctagc gcaaggcgcc tgcatggcgg
      721 cggaagacgc ggtggtgctg gcggaaaaag cggcggcatc gcccgacaac ctgccggcgg
      781 cgttcaaggc ctacgagcag aaccgctatc tgcgcaccgg ccgcgtgcag atcatggcgc
      841 gcgtctatgg cgagttttat cacgcgcgcg gcgtcgccgc cgagctgcgt gacatggcgc
      901 tcgccggccg cacgccggaa cagagctacg acggcatcgc ctggctttac ggcgggccgt
      961 aattagtact gcaaactaac gattgtgtac gtgcgagagc a
//
LOCUS       DASRVO010000700         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_254174, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000700 DASRVO010000000
VERSION     DASRVO010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254174"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFF05_08705"
     CDS             <1..252
                     /locus_tag="VFF05_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HZW51928.1"
                     /translation="YGLARNHPFVDGNKRTAAVACETLVELNGAEMRADDLELYPVYI
                     ALAEGSLSEAQFAAWLRQRIVPKCARGVHEPRARYAARR"
     gene            265..816
                     /locus_tag="VFF05_08710"
     CDS             265..816
                     /locus_tag="VFF05_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZW51929.1"
                     /translation="MIKINLGSGDHKLDGWLNVDIAARERPDLIADLAQPLPFVDGCA
                     DYIHTEDFFAALTLEQAHAFLRECRRVLKPQGAMRLLTPDLEKFARMYLERPQWLVEV
                     WKRSIDIPLRTGSACEVFNIGMRLGGQFHYDHSTLIDAAAACGLRAYAVAYAQSAHAA
                     LRNIDLRRPEETVSMYLECVRAD"
     gene            complement(912..>2754)
                     /locus_tag="VFF05_08715"
     CDS             complement(912..>2754)
                     /locus_tag="VFF05_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51930.1"
                     /translation="ICSPATQHYIYVGSGPSCQTTSIQTAIGSIACPDTSIVVSTALG
                     ETYTGQHITLNNVSANIVGSADQCDAPPVVCDPEIGCGGGGPPPKIAIGGDGANPVFY
                     VSGGGYVGFANLSISGGKGADNSLSGGGGIGVFGTATAVTLRNVELHDNTGGNGGGIA
                     FYGDGSLTLDGTTIQHNSVTGAGGGLSVDSSAIGHIDVTIADDPNLATDIHDNTATVM
                     GGGISVSGNVHLLAASANALIRNNHAGEFGGGIAVGGGTLADLGLAGNAISGNLSDFA
                     GGGIVVVPNTSGSPVVRLFNRDGSNPLNVQYNKAAQSAGGLYLYPGNASLHATACLFD
                     VDFTANHVLSEGSAILVGDYGRLRVNPTSDAECDPSAVMALGAKPNAYGGSHLYANVG
                     IDGSNQVTNAAVIDADGSNAQVSVQRLNLVQNSGGYAIHARNAAGSSLAFSQCLLEQN
                     YTLHEAVNLQGTAAGFDGCTFARNNINGSSVFAFDTGLSLTRSIVAEDGALQVWSPTS
                     APGLTAHYLALSGPNLSTDATVIYADPVFVDPDSGDFHLQITSPAADYAPNGNEAGNT
                     DLDGRPREVDKANAVNRFGPRDLGAYEISPACFQFDTVSCNGFESTQ"
BASE COUNT          477 a          837 c          908 g          532 t
ORIGIN      
        1 tatggactcg cgcgcaatca tccgtttgtc gatggcaata aacgcacggc ggcagtcgca
       61 tgtgaaactc tcgtcgaact caacggcgcc gagatgcggg cggacgatct ggagttgtat
      121 ccggtttaca ttgccctcgc agaaggctcc ttgtccgagg cgcaatttgc cgcttggctg
      181 cgccagcgca tcgtacccaa gtgcgcgcgc ggcgtacacg aaccgcgggc gcgttacgca
      241 gcccgacgtt gacgggggaa tcgcatgatt aaaatcaatc tcggcagcgg cgaccacaag
      301 ctggatggct ggttgaatgt tgacatcgcc gccagggaac gtcccgatct gatcgcagac
      361 ttggcgcagc cgctgccctt cgtcgatggc tgcgccgatt acatccacac cgaggatttt
      421 tttgctgcgc ttactcttga gcaggcgcac gcttttctgc gcgaatgccg gcgtgtgctc
      481 aaaccgcagg gcgcgatgcg cctgctcacg ccggacctgg agaagttcgc gcgcatgtat
      541 ctggagcgac cgcaatggct cgtggaggtg tggaagcgtt ccatcgacat tccgctgcga
      601 accggatcgg cctgcgaggt gttcaacatc ggcatgcgcc tgggcggcca gttccactat
      661 gatcattcca ccttgatcga cgccgctgcc gcatgcggat tgcgcgctta tgctgtggcc
      721 tacgcgcaga gcgcacatgc ggcgctgcgc aacatcgatc tacgccgccc ggaagagacc
      781 gtgagcatgt atctggagtg cgtgcgcgcg gattgacgcc actcaactcc tgcgccgtga
      841 gttgccggca ccggggattc cgcgttcgat gacgtattcg ggcatgacgg tttcccgggt
      901 tggatggggc atcactgggt cgattcgaag ccgttgcatg acacggtgtc gaactggaaa
      961 caggcgggcg agatttcgta cgcgcccaga tcgcgcgggc cgaaacgatt gacggcattg
     1021 gccttgtcca cctcgcgcgg gcgcccatcg aggtcggtgt tgcccgcctc gttgccgttc
     1081 ggcgcgtaat ccgccgctgg agaagtaatt tgcaagtgga aatcgccgct gtccggatcg
     1141 acgaaaaccg gatcggcgta aatcaccgtg gcatccgtcg acaggttcgg gccactcaac
     1201 gccagatagt gcgcggtcag gcccggagcc gacgtcgggc tccagacctg caacgcgccg
     1261 tcctcggcga cgatgctgcg ggtcagactc aggccggtat cgaaagcgaa aacgctggag
     1321 ccgttgatgt tgttgcgcgc gaacgtgcag ccgtcgaaac cggcggcagt gccttgcagg
     1381 ttcaccgctt cgtgcaaggt gtagttctgc tcgagcaggc actgactgaa cgcaagactg
     1441 gagcctgccg catttcgtgc gtggatcgca tagccgccgc tgttctggac gagattcagg
     1501 cgttgcactg acacttgtgc gttactgcca tcggcgtcga tgacggcggc gttggtgacc
     1561 tgattggagc cgtcgatgcc gacatttgcg tacagatggc tgccgccgta ggcattgggc
     1621 ttggcgccca aggccatgac tgcggacggg tcgcattcgg cgtcgcttgt cggattcacg
     1681 cgcaggcgac cgtagtcgcc gaccaggatt gccgaacctt cgctcagcac atggttggcg
     1741 gtgaaatcga catcgaaaag gcaggcggtc gcgtgcagac tcgcattgcc cgggtacagg
     1801 tacaggccgc cggccgattg cgccgccttg ttgtattgca cgttcagcgg attcgaaccg
     1861 tcacgattga acaggcgcac gaccgggctg ccgctggtgt tgggaacgac gacgatgccg
     1921 ccgccggcga agtccgatag gttgccggat atggcgttgc cggccaggcc gaggtcggcc
     1981 agcgtgccgc cgccgaccgc gatgccaccg ccgaactctc cggcatggtt gttccggatc
     2041 agcgcgttgg cgctggcggc cagcagatgc acgttgccgg aaacgctgat gccgccgccc
     2101 atgaccgtgg cggtattgtc gtgaatgtcc gtcgccagat tcggatcgtc ggcaatcgtc
     2161 acatcgatgt ggccgatggc agaggaatcg acgctcaagc cgccgccagc tcccgtgacg
     2221 gaattgtgct ggatcgttgt cccgtccagg gtcaggctgc catcgccgta gaaggcgatg
     2281 ccgccgccat tgccgccggt gttgtcgtgc aattcgacat tgcgcagcgt caccgcggtg
     2341 gcggttccga atacgccgat gccgccgccg ccgctgagcg agttgtccgc gcccttgccg
     2401 ccgctgatgg aaagattcgc gaacccgacg tagccgccgc cgctgacata gaacacggga
     2461 ttggcgccat cgccgccgat ggcgatcttc ggcggcggcc cgccgccgcc gcaaccgatc
     2521 tcgggatcgc acaccaccgg cggcgcgtcg cactggtccg cgcttccgac gatgttggcc
     2581 gagacgttgt tcaacgtgat gtgttggccg gtataggtct cgccgagcgc cgtgctgacg
     2641 acgatcgagg tatccggaca ggcaatgctg ccgatcgcgg tctggatgct ggtcgtctgg
     2701 catgaggggc cgctgccgac gtaaatgtag tgctgtgttg ccggcgagca gatc
//
LOCUS       DASRVO010000701         1439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_383947, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000701 DASRVO010000000
VERSION     DASRVO010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383947"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1038
                     /locus_tag="VFF05_08720"
     CDS             148..1038
                     /locus_tag="VFF05_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZW51931.1"
                     /translation="MDWQKGRQSDNVVQDDGGGGGGGFGGGGGMRFGGFHLGIGGTII
                     VVIIALIFPQTRPLIMNLFLSGGGTSDMTQSAPAPGQRHAADASDPQVQFVSTILAST
                     EDVWDPYFQSIGKQYTRPKLVLFHGQTSSGCGAAQAAMGPFYCPADKLVYLDLDFFKE
                     MDTRFHASGDFARAYVIAHEVGHHVQDLLGVMQQVGRPTEGASGTSVRLELQADCFAG
                     VWGNKSQAQLNWLQAGDIDAALNAAAQVGDDTLQKESRGYAVPDSFTHGTSAERSRWF
                     KAGFQSGDIRSCDTFNAATL"
     gene            1049..>1439
                     /locus_tag="VFF05_08725"
     CDS             1049..>1439
                     /locus_tag="VFF05_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HZW51932.1"
                     /translation="MMFAKELRRRLVAAPLLALLFALGGCVNAPVHPPAYSPLILISI
                     DGYRADYITRGLSPNLAALASEGVRATAMKPSFPTLTFPNHYSIVTGLYPDHHGIVNN
                     RFLDPVTDAKFVYNDRSTTNDPHWWAGG"
BASE COUNT          256 a          481 c          445 g          257 t
ORIGIN      
        1 cctgcaccgg gccgctcgag actcgatgga ttacaacgaa gtgcgagcca ggcatcgcag
       61 cgcgtcagcc agaggcagcg cgcttgcgcg tttcgccggc atcggagtag tgttcgccct
      121 ggatttccac gcggggcggg aggacgtatg gattggcaaa agggtcggca aagcgacaat
      181 gtcgttcagg acgatggcgg tggtggtggt ggcggattcg gcggtggcgg cggcatgcgc
      241 ttcggcgggt tccacctcgg catcggcggc acgatcatcg tcgtcatcat cgcgctgatc
      301 ttcccgcaga cgcgtccgct gatcatgaac ctgttcctga gcggcggcgg cacctcggac
      361 atgacccaat ccgcgccggc accgggtcag cgccacgcgg ccgacgccag cgatccgcaa
      421 gtccaattcg tttccacgat actcgccagc accgaggacg tctgggatcc ttatttccag
      481 tccataggca agcaatacac gcggcccaag cttgtgctgt tccacggcca gacctcgtcc
      541 ggctgcggtg cggcgcaggc ggcgatggga ccgttctact gcccggccga caaactggtg
      601 tacctcgacc tcgatttctt caaggaaatg gacacgcgct tccacgcatc tggcgatttc
      661 gcgcgcgcct acgtgatcgc gcacgaggtc ggccaccacg tgcaggacct gctcggcgtc
      721 atgcagcagg tcggccggcc gaccgaaggc gcgagcggca cgtcggtacg cctcgaacta
      781 caggccgact gttttgccgg cgtctggggc aacaagtcgc aggcgcagtt gaactggctg
      841 caggccggcg acatcgacgc ggcgctgaac gccgccgccc aggtcggcga cgataccctg
      901 cagaaggaat cgcgcggtta cgccgtgccg gattcgttca cccacggcac ctcggcggag
      961 cgctcacgct ggttcaaggc gggattccaa tccggtgaca tccgcagttg cgataccttc
     1021 aatgccgcca cgctctgaag cgtaacccat gatgttcgcg aaagaacttc ggcgccgcct
     1081 cgtcgcggcg cccctgctgg cacttttgtt cgcgctcggc ggatgcgtga acgcgccggt
     1141 gcatccgccc gcgtattcgc cgctgatcct gatttccatc gatggctacc gcgccgacta
     1201 catcacgcgt ggtctgtcgc caaatctcgc ggcactcgca tccgaaggcg tgcgcgccac
     1261 ggcgatgaaa ccgtcgtttc caacgctcac gtttccaaat cactacagca tcgtcaccgg
     1321 cctgtatccg gaccatcacg gcatcgtcaa caatcgtttc ctcgacccgg tcactgacgc
     1381 gaagttcgtc tacaacgacc gcagcaccac gaacgacccg cattggtggg ccggcggcg
//
LOCUS       DASRVO010000702         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_20638, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000702 DASRVO010000000
VERSION     DASRVO010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20638"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFF05_08730"
     CDS             <1..531
                     /locus_tag="VFF05_08730"
                     /inference="COORDINATES: protein motif:HMM:NF037683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51933.1"
                     /translation="ANGKSALVGLRMNRAADFGDLLPFYIRYATLRLSDFAVEPIFLE
                     QAESGSVREPALLAAIRIQDAGQLRSGLQALLALARQSQHAPLPFPAKTAWAWCTAKS
                     DARLDKAGKAWRDDDGERGESRYAPGYNALFLRGADFPDGDGAAALAFARTCARVAAV
                     LDPQRTILLRETEPTP"
     gene            528..>1050
                     /locus_tag="VFF05_08735"
     CDS             528..>1050
                     /locus_tag="VFF05_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZW51934.1"
                     /translation="MTKDFIRTLDWQSLSLTAPDRLLIEASAGTGKTWTIAALYLRLL
                     LEGENPPAVEEILVATFTEAAARELRERLRVRLAEAERELQRIRGGAVREAGDVDSVL
                     KWLADEFTGESETKRALRRVQLARMDFDRAPIGTIHALCQRFLQDFPLDSGIAAAQAN
                     LDEDELLRECVEDF"
BASE COUNT          178 a          332 c          355 g          185 t
ORIGIN      
        1 gcgaatggca agtccgcatt ggtcggattg cgcatgaatc gcgcggcgga ttttggcgac
       61 ctgctgccgt tctacatccg ctacgcgaca ttacgcttga gcgattttgc cgtcgaaccg
      121 atttttctgg agcaagccga atccggttcg gtgcgcgaac cggcattgct cgcggccatt
      181 cgtattcagg acgcggggca actgcgcagc ggactgcaag ccctgctcgc gctggcgcgg
      241 caatcgcagc atgcgccttt gccgtttccc gcaaaaaccg cctgggcctg gtgcaccgca
      301 aaatccgacg cccggctgga caaggcaggc aaggcgtggc gcgacgacga tggcgaacgc
      361 ggcgaaagcc gctacgcgcc cggctacaac gcgcttttcc tgcgtggcgc cgattttccg
      421 gatggcgacg gtgcggcggc gctggcgttt gcgcgaacct gcgcgcgggt ggcggccgtg
      481 ctcgatccgc agcgcacgat cctgttgcgc gaaacggagc cgacgccgtg acgaaggatt
      541 ttatccgaac cctggactgg caatcgctgt cgctgaccgc gccggatcgc ctgttgatcg
      601 aggccagcgc cggcaccggc aagacctgga ccatcgctgc gctgtacctg cgactgctgc
      661 tcgaaggcga gaacccgcca gcggtcgaag agattcttgt cgccaccttc accgaggcgg
      721 ccgcgcgcga gttgcgcgaa cgtctgcgcg tgcgactggc cgaggccgag cgcgagttgc
      781 aacgcatccg cggtggcgcg gtacgcgaag cgggcgacgt cgactccgtt ctgaagtggc
      841 tggcggacga attcactggg gaaagcgaga cgaagcgggc actgcgccgc gtgcaacttg
      901 cgcgcatgga tttcgaccgc gcgcccatcg gcacgattca tgcgttgtgc cagcgcttcc
      961 tgcaggattt tccgctggat tccggcatcg ccgccgcgca ggcgaatctg gacgaagacg
     1021 agctgctgcg cgaatgcgtc gaggatttct
//
LOCUS       DASRVO010000703         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_281061, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000703 DASRVO010000000
VERSION     DASRVO010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281061"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /locus_tag="VFF05_08740"
     CDS             <1..2063
                     /locus_tag="VFF05_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW51935.1"
                     /translation="SGELPAALAAVSAAPAVAAVEAPTPAATTAPMHVPEAAAVKPAA
                     RPAPRALPAIEEEVQQHAPQEMIRVRSDLLDSLVNYAGEVSIYRSRLEQQISTFRFNL
                     VEFEQTVSRLRDQLRKLELETEAQILSRYQREAESSESTFDPLELDRFSQLQQLSRAL
                     AESVSDLVSIQGLLDDLTRQSETLLLQQSRVSSDLQEGLMRTRMVPFDSLVPSLRRTV
                     RQAAQETGKRAQLKVEGAQGEMDRNLLERMKAPFEHMLRNALAHGIETPDERAAGGKP
                     AEGTVTIHVSREATEVVLRVADDGRGMDRDAIRRKAIERGLLKPDVQLSDRDLYGFIL
                     ETGFSTAEQVTQLAGRGVGMDVVHNEIKQLGGSLAIDSVRGAGSTFIIRLPFTLAVTQ
                     AILVRLGEATYAVPMSSVQGVVRIAHDDLERRMSQPNPAYNYAGEEFLIYELSQLLNV
                     PASRMADETQLPLLMTRTGDQRAAVRIDSVIGSREIVVKSVGPQISSIPGIFGATIMG
                     DGSVVMILDLAPLVRRSAALRERAEAAGEAVMLPAAPVAPVEPAIEQRAHPLIMVVDD
                     SITMRKVTTRVLERNDMDVVTAKDGLDAVEKLQDKVPDVVLLDVEMPRMDGYELATYM
                     KNDARLKQVPIIMITSRTGEKHRQRAFEIGVERYLGKPYQEADLLRNVQETLSAKNPG
                     LHSA"
     gene            2056..>3207
                     /locus_tag="VFF05_08745"
     CDS             2056..>3207
                     /locus_tag="VFF05_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51936.1"
                     /translation="MPESNAIAVALLYQSGQLGSHLKNALGELGAAVVYEAPPANIDR
                     DALENSGARVVIVNLDADSDNYIDALYDVLDAGDYEVVFNDAQVSSNLQGWDQARWVR
                     HLGSKILRKPENVEPPRPPGAQAVPTPVQKSASSVPIAEKPGPVPSMPVAPLPVVEEV
                     PASERPTVKMPLPPELLAQPPAASEAPPQATAASVDAAELAEFAKLLDTPATAGAPEP
                     VATAPAPIPELGDFAAELDALFAAADTKSAQAPAAAAKAAASDEFDIALPADFDLALD
                     EVAPVPAMELDALDIPVDTTAAPVPPKTEDVAELHDEFPAIFDQLDVDAAAVPEAKNP
                     EFKPAERPAAEPESEPVLPMEWSLQGDDAQPVTGKASFGIEKMSAEEFLA"
BASE COUNT          581 a         1067 c         1045 g          514 t
ORIGIN      
        1 tttccggcga attgcctgcg gcgttggcag cagtttccgc tgcgcccgcc gttgcggccg
       61 tcgaagctcc aactcccgcc gcgacaacgg cgccgatgca tgtgcccgaa gcggcggcgg
      121 tgaaacctgc cgcgcgcccg gctccgcgcg cgttgcccgc gatcgaggaa gaagtccagc
      181 agcacgcgcc gcaggaaatg atccgcgtgc gctcggacct gctcgattcg ctggtcaact
      241 acgcgggcga agtgtcgatc taccgttcgc gcctggaaca gcagatctcg accttccgct
      301 tcaacctggt cgagttcgaa cagaccgtca gccgcctgcg cgatcagttg cgcaagcttg
      361 aactggaaac cgaagcgcag atcctgtcgc gctaccagcg cgaggccgaa tcttccgaat
      421 ccacgttcga tccgctggaa ctggatcgtt tcagccagtt gcagcagctc tcgcgtgcgc
      481 tcgccgaatc ggtatccgac ttggtttcga ttcagggctt gctcgacgac ctgacgcgcc
      541 agtccgaaac cttgttgttg cagcagtcgc gtgtttcgtc cgacttgcag gaaggcctca
      601 tgcgcacgcg catggtgccg ttcgactcgc tggtgccatc cttgcgccgc accgtgcgcc
      661 aggccgcgca ggaaaccggc aagcgcgcgc agttgaaagt ggaaggcgcg cagggcgaaa
      721 tggaccgcaa cctgctcgag cgcatgaagg cgccgttcga gcacatgttg cgcaacgcgc
      781 tcgcgcacgg catcgagacg cccgacgagc gcgccgccgg cggcaagccg gccgagggca
      841 cggttacgat tcacgtcagc cgcgaggcga cggaagtcgt gctgcgcgtg gccgacgacg
      901 gacgcggcat ggatcgcgac gcgatccggc gcaaggcgat cgaacgcggc ctgctcaaac
      961 ccgacgtgca attgtccgac cgcgatctct atggcttcat cctggaaacc ggtttttcca
     1021 ctgccgaaca ggtgacccag ctcgccggcc gcggcgtcgg catggacgtg gtgcacaacg
     1081 agatcaagca actcggcggt tcgctcgcca tcgattcggt gcgcggcgcc ggcagcacgt
     1141 tcatcatccg cctgccgttc acgctggcgg tgacgcaggc gattctggtg cgcctcgggg
     1201 aggcgaccta cgcggtgccg atgtcctcgg tgcagggcgt ggtgcgtatc gcgcacgacg
     1261 acctggaacg gcgcatgagc cagcccaatc ccgcttacaa ctatgctggc gaggaattcc
     1321 tgatctacga attgtcgcaa ctgctcaacg tgccggcttc gcgcatggcc gacgagacgc
     1381 agctgccgct gctgatgacg cgcaccggcg accagcgtgc cgccgtgcgc atcgacagcg
     1441 tcatcggttc gcgcgaaatc gtggtcaagt cggtcggtcc gcagatcagc tcgatcccgg
     1501 gcatcttcgg cgcgacgatc atgggcgacg gttcggtcgt catgattctc gacctcgctc
     1561 cgctggtacg ccgcagcgct gcgttgcgcg agcgcgcgga agccgcgggc gaggccgtga
     1621 tgctgccagc ggcgccggtc gctccggtcg agcccgcgat cgagcagcgc gcgcatccgc
     1681 tgatcatggt ggtggacgat tcgatcacca tgcgcaaggt caccacgcgc gtgctcgaac
     1741 gcaacgacat ggacgtggtc accgcgaagg acggcctcga cgcggtcgag aaattgcagg
     1801 acaaggtgcc ggacgtggtc ctgctcgacg tggaaatgcc gcgcatggac ggctacgagc
     1861 tcgccaccta catgaagaac gatgcgcgcc tgaaacaggt gccgatcatc atgatcacct
     1921 cgcgcaccgg cgagaagcac cgccagcgcg cgtttgaaat cggcgtggag cgctacctcg
     1981 gcaagcccta tcaggaagcc gatctgttgc gcaacgtgca ggaaaccttg agcgccaaga
     2041 acccgggatt gcacagtgcc tgagtccaac gccatcgcgg ttgcgctgct gtaccagtcc
     2101 gggcagcttg gcagccatct gaaaaacgcg ctcggcgagc tcggtgccgc cgtggtctac
     2161 gaggcgccgc cggcgaacat cgaccgcgat gcgttggaga attccggcgc gcgcgtggtc
     2221 atcgtcaacc tcgatgccga cagcgacaat tacatcgacg cgctgtacga cgtgctcgat
     2281 gccggcgatt acgaggtcgt cttcaacgac gcgcaggtct cgtcgaacct gcaaggctgg
     2341 gatcaggcgc gctgggtacg ccatctgggt tccaagatcc tgcgcaagcc ggagaacgtc
     2401 gaaccgccgc gcccgcccgg cgcgcaggcg gtgccgacac cggtgcagaa atcggcgtcc
     2461 agcgtgccga tcgcggaaaa gcccggaccg gtgccttcca tgcccgtcgc gccgcttccc
     2521 gttgtggaag aagtgccggc gtcggagcgt ccaaccgtca agatgccgct gccgccggaa
     2581 ttgctcgcgc agccgccggc agcgagcgaa gcgccaccgc aggcaactgc cgcgagcgtc
     2641 gatgcggccg aactcgccga gttcgccaaa ttgctcgaca cgccggccac cgcaggggcg
     2701 ccggaaccgg tcgcgacggc tcccgcgccc attccggaac tgggcgattt cgccgccgag
     2761 ctcgatgccc tgttcgccgc ggcggacacc aagtctgcgc aggcaccggc agcggccgcg
     2821 aaagcggcag cgtccgatga attcgatatc gccttgccgg cggatttcga tctggcgctt
     2881 gacgaggtcg cgcccgtgcc cgcgatggaa ctggatgcac tcgacattcc cgtggatacg
     2941 accgccgcgc cggtgccgcc gaaaacggaa gatgtcgccg agttgcacga cgaatttccc
     3001 gccatcttcg atcaactcga tgtcgatgct gccgcggtgc ccgaggcgaa gaacccagaa
     3061 ttcaagccgg ccgagcgccc ggccgcggaa cccgaatccg aaccggtgtt gccgatggaa
     3121 tggtcgctgc aaggcgacga tgcgcagccg gtaaccggca aggccagctt cggcatcgaa
     3181 aagatgtcgg ccgaggaatt cctcgcc
//
LOCUS       DASRVO010000704         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_307093, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000704 DASRVO010000000
VERSION     DASRVO010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307093"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VFF05_08750"
     CDS             complement(<1..1223)
                     /locus_tag="VFF05_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51937.1"
                     /translation="MRKNQARPIPHGGSAPVEQPGSGPGGILGLRAFLIERMAMFAVA
                     PLIGLISGFVLLFGGIFLAVAWTVGPKPFLDARQYAPYTAHADGHIVESWIALEFDPS
                     ILPKDKLYWQPWSKVTPCAIVEYGSDWGAPLQRGFCGNRFDFRDDFRFDDWHTLMPDV
                     PFSFARDANGFAIQELRLSKTALDWLSQHPPHDTFMLPKPPPTTALGALKHQFDAPLE
                     VAVSSWTTPLRTLPLAYDPQHPEAAVPAQVVENARGGAWWLGMVFAVIFVVPGLFVFR
                     IGLSLLTGQSGALLWVLTALVLVALPWWGELLPRLVASANREWASIASDMLDDLSRVT
                     RFTASAPGEALLRGGERIVWKTDDGEYADTFGRLHFTLPNPAPKSDAEALAALRAQAT
                     QQVGTFDSTQRAELFL"
     gene            1379..2434
                     /locus_tag="VFF05_08755"
     CDS             1379..2434
                     /locus_tag="VFF05_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZW51938.1"
                     /translation="MRVMIVSLSPLWKEPPRLARALAEHGATVASLAIAGSLLDYSRF
                     IARRYRLGDDSPTQNNELVAAISAFAPDVLLPGDECSVRWLHAVYREAAVDASVKALI
                     AESLGDPQWYGRTTDKLAMSRLAHELGIAQLETTALGCTEDAYAFVQRHGWPVVIKAC
                     RGFAGIGVHPCANLRELRQTLHHIRAPSPQLIQRFVPGTTWMTAFVAHRGEVLGALVA
                     EKQRQHPALIGPSTIVRFAHDPAMLEVTHTLVHRLGYSGFGSVDFLRTQGRDPLFIEF
                     NPRATPICHLGGKLGVDLAGAFVHRRACDSAGLRNERVALFPQELQRDRSGAGLVGCW
                     HDIPEDEPELIAAMLAA"
BASE COUNT          457 a          867 c          808 g          416 t
ORIGIN      
        1 aggaacagct cggcgcgctg cgtggaatcg aatgtcccga cctgctgcgt tgcctgcgcg
       61 cgcagcgcgg ccagcgcttc ggcatcggac tttggtgccg ggttcggcag cgtgaaatgc
      121 aggcgcccga acgtgtccgc atactcgccg tcatcggttt tccagacgat gcgctccccg
      181 ccgcgcagca acgcctcgcc cggcgcgctc gcggtgaaac gcgtgacccg gctgaggtcg
      241 tcgagcatgt cgctggcgat gctcgcccac tccctgttcg cagacgccac aaggcgcgga
      301 agtagttcgc cccaccacgg caacgcaacc agcactaacg ccgtcagcac ccacagcagc
      361 gcgccggatt gcccggtcag cagcgacagg ccgatgcgga acacgaacaa tccgggaacc
      421 acgaagatca cggcgaagac catgccgagc caccaggctc cgccacgcgc gttctcgacg
      481 acctgtgcgg gcacggcggc ctccggatgt tgggggtcgt aagcgagtgg aagggtccgt
      541 agcggcgtgg tccaactcga caccgccact tccagtggcg cgtcgaactg atgtttcagc
      601 gcgccaagcg ccgtggtggg cggcggcttc ggcagcataa aggtgtcgtg cggcggatgt
      661 tgcgatagcc agtccaacgc agtcttgctg agacgcagct cctggatcgc gaacccgttc
      721 gcgtcgcgcg cgaaggagaa cggcacgtcc ggcatgagcg tatgccagtc gtcgaagcgg
      781 aaatcatcgc ggaagtcgaa gcgattgccg caaaatccgc gttgcagcgg cgcgccccaa
      841 tcgctgccgt attcgacaat cgcgcagggc gtcaccttgc tccacggctg ccagtagagt
      901 ttgtctttgg gcaagatcga cggatcgaat tccagcgcga tccagctttc cacgatatgg
      961 ccgtccgcat gcgcggtgta cggcgcgtac tggcgcgcgt cgagaaatgg tttgggtcca
     1021 accgtccagg ccacggcgag aaaaatgccg ccgaacaaca gtacgaatcc ggaaatcaaa
     1081 ccgatcagcg gtgcgacggc aaacatcgcc atgcgctcga tcaggaaggc gcgcagaccc
     1141 aggatgccgc ccgggccact tcccggctgc tcgacgggcg cggatccacc atgcggaatt
     1201 ggcctggctt gattcttgcg catttcccca cctcccccat ggtgctgatg gacaggccgc
     1261 gccatgcgcg cagcccgatg gccaaggtta tagaataaat cgagtcattg ccggcgctgg
     1321 cgctagggga agttgcgcgc agccaaggtc tggccggcgt ggctttccgg gggatttcat
     1381 gcgggtaatg atcgtctcgt tgtcgccgct gtggaaggag ccgccacggc tggcgcgcgc
     1441 gctggccgaa cacggcgcga ccgtcgccag ccttgcgatc gcgggcagcc tgctcgacta
     1501 ctcgcgcttc atcgcgcggc gttatcggct cggcgacgat tccccgacgc agaacaacga
     1561 actcgtcgcc gcgatcagcg ctttcgcccc cgatgtcctg ctgcccggcg acgagtgcag
     1621 cgtgcgctgg ctgcacgccg tgtaccggga agcagcggtc gacgcgtcgg tgaaggcgtt
     1681 gatcgccgaa tcgctcggcg atccgcaatg gtacgggcgc acgaccgaca agctggccat
     1741 gtcccgactg gcgcacgagt tgggcatcgc acaactggaa accacggcgc ttggctgcac
     1801 cgaggatgct tacgccttcg tgcagcgcca tggctggccg gtcgtcatca aagcctgccg
     1861 tggctttgcg ggcatcggcg tccatccgtg cgccaacctg cgcgaattac gccaaactct
     1921 gcaccatatc cgcgcgccga gcccacagct gatccagcgc tttgtgcccg gcaccacgtg
     1981 gatgaccgcc ttcgtcgcgc atcgaggcga agtgctcggc gcactggtcg ccgaaaagca
     2041 gcgccaacat ccggccctga tcgggcccag cacgatcgtc cggttcgcgc acgatccggc
     2101 catgctcgag gtcacgcaca ccctcgtgca ccggctcggt tacagcggat tcggcagcgt
     2161 cgatttcctg cggacgcagg gccgcgatcc gctgttcatc gagttcaatc cgcgcgcgac
     2221 tccaatctgc catctcggcg gcaagctcgg cgtggacctc gccggcgcct tcgtgcatcg
     2281 ccgcgcctgc gacagcgccg gcttgcgcaa cgaacgcgtc gcactgttcc cgcaggagct
     2341 gcagcgcgat cgcagcggcg ccggcctggt cgggtgctgg cacgacatcc ccgaagacga
     2401 accagaactc attgccgcca tgctggccgc ttgacgccgc gggcatttgc cagattcgca
     2461 taatcgcgga caatgcccct ttcccgaagg acggccccat gcacaacgcc acctacgccc
     2521 ggctcaaggc cgaactcgac gcaatccg
//
LOCUS       DASRVO010000705         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_72817, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000705 DASRVO010000000
VERSION     DASRVO010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72817"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFF05_08760"
     CDS             complement(<1..254)
                     /locus_tag="VFF05_08760"
                     /inference="COORDINATES: protein motif:HMM:NF021369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HZW51939.1"
                     /translation="MKTHGNILHAGIAALAALLVLQIVWHAWLFPPAATQRWPTLALA
                     VLPLVPGLWIAGRNLRRGVLVGGIVSLFYFCHGVSAAWGDP"
     gene            complement(251..844)
                     /gene="wrbA"
                     /locus_tag="VFF05_08765"
     CDS             complement(251..844)
                     /gene="wrbA"
                     /locus_tag="VFF05_08765"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014506797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HZW51940.1"
                     /translation="MAEILIVYYSRNGSVAQLARHVARGVEEVPDMRARLRSVPPVAP
                     VTQVAQPPEPAEGAPYATLADLRECAGIILGSPTRFGNMAAPLKHFLDSSGAEWASGA
                     LVGKPAAMFTSTSTLHGGQESTLLSMLMPLLHHGALIVGIPFTEPEINTTTTGGTPYG
                     ASHVAGASGDRAISDDERTLARKLGRRVAEIAAKLGT"
     gene            880..1770
                     /locus_tag="VFF05_08770"
     CDS             880..1770
                     /locus_tag="VFF05_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HZW51941.1"
                     /translation="MLKSQRHRAATFARFTARRFLDENCLQTAAALAFTSLFALVPLI
                     TVVLGILSAFPVYSQWRESITRFVFDNFLPATGEAVQGYFTQFADNASKATAVGVLVL
                     VFSAVSLMLSIEGAFNRIWRVRQMRRAATRFAIYWTTLTLGPFLLVTMLALMSWAFAS
                     PVAESAVANSWLRSHLLGVAPFLLQWLVLAMAYALIPSCAVRPRHAALAALLIALAFE
                     AAKRGFVLYAGHSYEEIYGVVAVVPLFIFWIYLAWILVLLGASLTASLTAFAGHVRET
                     IVPSPMSESALDDSKLEKIS"
     gene            1767..>2210
                     /locus_tag="VFF05_08775"
     CDS             1767..>2210
                     /locus_tag="VFF05_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZW51942.1"
                     /translation="MKLAAILALVLCSGVALAAAPKPALSVKTLDGGTFDLASQQGKW
                     VIVNFWATWCSPCLKELPDISAYVAAHKDKVAAIGLDFEDADKADIEKFLTSHPLSFP
                     VSMVDVDNPPKDFGAPKGLPNTYVIAPDGHLAKTFLGPIQTRDLQD"
BASE COUNT          341 a          722 c          722 g          425 t
ORIGIN      
        1 ggatcgcccc aggccgcgct gacaccgtgg cagaagtaga acaggctcac gatgccgccg
       61 acgagcaccc cgcggcgcag gttgcggcct gcaatccaca agccgggcac gagcggcagc
      121 acagccaggg ccagggtcgg ccagcgctgc gtggcggctg gtggaaacag ccaggcgtgc
      181 cagacgattt gcaacaccag cagtgcggcc agcgccgcga tgccggcatg caggatgtta
      241 ccgtgcgtct tcatgtgccg agcttcgccg cgatttccgc cacgcgccgg ccgagcttgc
      301 gcgccagcgt gcgctcgtcg tcgctgatgg cgcggtcgcc ggatgcgccc gcgacatggc
      361 tggcgccata cggcgtgccg ccggtcgttg tcgtattgat ctcgggttcg gtgaacggga
      421 tgccgacgat caacgcgccg tggtgcagca gcggcatcag catcgacagc agagtgctct
      481 cctgtccgcc gtgcagggtc gaggtcgagg tgaacatggc ggcgggtttg ccgacgagcg
      541 cgccggatgc ccattccgcg ccgctggaat ccagaaaatg tttcagcggt gcggccatgt
      601 tgccgaagcg cgtcggactg ccgaggatga tgccggcgca ttcgcgcagg tcggcgagcg
      661 tcgcgtacgg cgcgccttcg gcaggttcgg gcggctgcgc gacctgggtg accggcgcga
      721 ccggtggcac gctgcgcagg cgtgcgcgca tgtcggggac ttcctcgacg ccgcgcgcaa
      781 cgtggcgcgc gagctgggcc acactgccgt tgcggctgta gtagacgatc aggatttcag
      841 ccatcgctgt ctcgcggtga tttgaactac tatcatcgca tgttgaaatc gcaacgccac
      901 cgcgccgcga ccttcgcgcg ctttactgcg cggcgcttcc tcgacgaaaa ctgcctgcaa
      961 accgcggcgg ccctggcatt cacgagcctg ttcgcgctcg tgccactgat cacggtcgtg
     1021 ctgggcatcc tgtcggcgtt tcccgtgtac tcgcagtggc gcgaatccat cacgcgcttc
     1081 gtcttcgaca atttcctgcc ggctaccggc gaggccgtgc agggctactt cacccagttc
     1141 gccgacaatg cgagcaaggc cacagcggtg ggcgtcctgg tcctggtctt cagcgccgtt
     1201 tccctcatgc tgagcatcga gggcgcgttc aatcgcatct ggcgcgtgcg ccagatgcgc
     1261 cgtgccgcca cgcgcttcgc catctactgg acgacgctca cgctgggtcc gttcctgctg
     1321 gtgacgatgc ttgcgctcat gtcctgggcg ttcgcatcgc ccgttgccga aagcgcggtc
     1381 gcgaattcct ggctgcgttc gcatctgctg ggtgtcgcgc cattcctgct tcaatggctg
     1441 gtcctggcga tggcctatgc gctgattccc agttgcgcgg tgcgcccgcg ccacgcggcc
     1501 ctggccgctc tgctcatcgc gctggcgttc gaggcggcca agcgcggttt cgtgctttac
     1561 gcaggacaca gctacgagga aatctacggc gtggtcgcgg tggtgccgct gttcatcttc
     1621 tggatatacc tcgcctggat cctggtgttg ctcggcgctt cgctcaccgc tagcctgacc
     1681 gcctttgccg gccacgttcg cgaaaccatc gtgccgtcgc cgatgtccga atccgcgctg
     1741 gacgattcca aattggagaa aatctcatga agcttgccgc catcctcgcc ctcgtgttgt
     1801 gttccggtgt cgcgttggcc gccgcgccga aacccgcgct gagcgtgaaa acgctcgacg
     1861 ggggaacctt cgacctggct agccagcagg gcaagtgggt gatcgtgaac ttctgggcga
     1921 cctggtgctc gccgtgcctg aaggaattgc cggacatctc cgcctatgtc gccgcgcaca
     1981 aggacaaggt cgctgcgatc ggtttggatt tcgaggatgc cgacaaggcc gatatcgaga
     2041 aatttctcac gtcgcacccc ttgagctttc cggtgagcat ggtcgatgtc gacaacccgc
     2101 cgaaggattt cggtgcgccc aagggtttgc ccaataccta cgtgatcgcg ccggacgggc
     2161 acctggcgaa gaccttcctc ggaccgatcc agaccaggga cctgcaggac
//
LOCUS       DASRVO010000706         1389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_384083, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000706 DASRVO010000000
VERSION     DASRVO010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384083"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1356
                     /locus_tag="VFF05_08780"
     CDS             286..1356
                     /locus_tag="VFF05_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW51943.1"
                     /translation="MALVAAVFAACSEKPATPRGEAVPVEIARVVQKAMPELVQAVGT
                     VEAIDSVAVKSLVDGQLLEVHVKDGDAVTRGELLFTIDPRPAQAALAQVQAALAKDVA
                     ARDLAHAQVERYAPVAKKGFISADQMQQYVTAEAAAAASVKVDQANVEAAKLALGYTR
                     ITAPMDGRAGRVLVQAGNLVKANDTNPLLVINRIAPIYVNFAVPGRDVDRVRAAQAQG
                     ELPAQAWVEGAASRTNGKVAFVDNAVDPATNTVKLRAQFANSDQALWPGQFVDVSMTL
                     ATQADALAIPVTAVKQGPDGDYVFVVKPDSRAEQRSVKVARDVDGEAVIANGLSAGEM
                     VVTDGQSRLADGAPVRIASPSN"
BASE COUNT          248 a          427 c          490 g          224 t
ORIGIN      
        1 cgggcaacgt gttccagaaa gcgcgacgat tcacatcgcg cggcagggga gcgtcgaaca
       61 attgcatgcg tgtcgccaac gaagacagcg ccaaaaagca ttttcatgcc ggcgcgggac
      121 gcgcagttta gcgtgtgtag caggcacgcg cagagacgcc atgactttcg gcagggtggc
      181 gcaggtttca caagtcttta cgcagcccgg ccttggcccg ggcgataatc gcggccattg
      241 cgatttttgc cgcgggagat cgtgttgcat cggatacgct ttggaatggc gctcgtcgcc
      301 gccgtgttcg ccgcctgttc ggaaaaaccg gccacgccgc gcggtgaggc cgtgccggtg
      361 gaaatcgcgc gcgtcgtgca gaaggcgatg cccgaactcg tgcaggccgt gggcacggtc
      421 gaggcgatcg acagcgtcgc ggtgaaatcg ctggtcgatg gccagttgct tgaagtgcac
      481 gtcaaagatg gcgatgccgt tacgcgcggc gagttgttgt tcacgatcga cccgcgcccg
      541 gcgcaggccg cgctggcgca ggtgcaggcg gcgctggcca aggacgtggc cgcgcgcgac
      601 ctcgcccatg cgcaggtcga gcgctacgca ccggtcgcaa agaaaggctt catttccgcg
      661 gaccagatgc agcagtacgt gaccgcggaa gccgcggcgg cggcgagcgt caaggtcgac
      721 caggcgaatg tcgaggccgc gaaactcgcg ctcggctaca cgcgcatcac cgcgccgatg
      781 gacggccgcg ccggacgcgt gctggtgcag gccggcaacc tggtcaaggc gaacgatacc
      841 aatccgctgc tcgtgatcaa ccgcattgcg ccgatctacg tgaacttcgc cgtacccggt
      901 cgcgacgtcg atcgtgtgcg tgcggcccag gcgcaaggcg agttgccggc gcaagcctgg
      961 gtcgagggcg ccgcgtcgcg cacgaatgga aaagtggcct tcgtggacaa cgcggtcgat
     1021 ccggccacga acacggtcaa gctgcgcgcg caattcgcga actcggatca ggcgttgtgg
     1081 cccggacagt tcgtcgacgt gtcgatgacg ctcgcgacgc aagccgatgc gctcgcgatc
     1141 ccggttaccg cggtcaagca gggtccggat ggcgactacg tcttcgtcgt caagcccgac
     1201 tcgcgtgctg aacagcgcag cgtgaaagtc gcgcgcgacg tggacggcga agccgtgatc
     1261 gcgaacggat tgagcgccgg cgagatggtg gtgacggatg ggcagtcgcg ccttgccgac
     1321 ggcgcgccgg tgcgcatcgc gtcgccctcg aactgagcgt cgccgtgagc gtttccgaaa
     1381 ttttcgtgc
//
LOCUS       DASRVO010000707         1252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_175246, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000707 DASRVO010000000
VERSION     DASRVO010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175246"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1252
                     /locus_tag="VFF05_08785"
     CDS             <1..>1252
                     /locus_tag="VFF05_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007080873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZW51944.1"
                     /translation="AVIITLMLTLFASWAMGFTINRVSLFALIFSIGILVDDAIVVVE
                     NIHRHMALGGRSLREAIPEAVAEVGGPTILATFTVIAALLPMAFVSGLMGPYMRPIPI
                     NASAGMLLSLAVALVVTPWLSLKLIGRHHAAVADAQGEAPRASRLLRLFQRLMMPFLD
                     AGRGRRRRRLLFAAMGALVMLAASLVVFRAVVLKMLPFDNKSEFQVVVDMPEGNTLET
                     TNRLLSDLAHALDSVPEVRNYQAYAGTAAPINFNGLVRQYYLRSAPIDGDLQVNLVDK
                     HERKRKSHEVALAVRPMLAKIGARYGASVKVVEVPPGPPVMAPIVAEVYGPDYAQLRK
                     IALGLEKDFHSDPDLVDVDTSVEADSPRDVVIVDRARAARLGVSQSDIARTLAAALSG
                     DDATYLMDGRSKYAVPLRLRLPAGA"
BASE COUNT          192 a          430 c          410 g          220 t
ORIGIN      
        1 gcagtcatca tcaccctgat gctgaccctg tttgcttcat gggcgatggg cttcacgatc
       61 aaccgcgtat cgctgttcgc cctgatcttt tcgatcggca tcctcgtcga cgacgccatc
      121 gtcgtggtcg agaacatcca ccgccacatg gcgctcggcg ggcgcagcct gcgcgaggcg
      181 attcccgaag ccgtcgccga agtcggcgga ccgacgatcc tggccacgtt caccgtcatc
      241 gctgcgctgc tgccgatggc gttcgtgtcg gggctgatgg gtccgtacat gcggccaatc
      301 ccgatcaacg cctcggccgg catgctgctg tcgctggcgg tcgcgctggt cgtcacgccg
      361 tggctgtcgc tgaaactgat cgggcgtcac cacgccgcgg ttgccgacgc gcaaggcgag
      421 gcgccacgcg caagccgcct gctgcgcctg ttccagcgcc tgatgatgcc gttcctggat
      481 gccggccgcg gccggcgccg gcgccgcctg ctgttcgcgg cgatgggcgc gctggtgatg
      541 ctggcggcca gcctcgtcgt gttccgcgcg gtggtcctga agatgttgcc gttcgacaac
      601 aagtccgaat tccaggtcgt cgtcgacatg cccgaaggca acacgctgga aacgacgaac
      661 cgcctgctgt ccgatctcgc gcatgcgctc gatagcgtcc ccgaagtgcg caactaccag
      721 gcctatgccg gcaccgccgc accgatcaac ttcaacggcc tggtgaggca gtattacctg
      781 cgcagcgcgc cgatcgacgg cgacctgcag gtgaacctcg tcgacaagca cgaacgcaag
      841 cgcaagagcc acgaggtcgc gctggcggtg cgcccgatgc tggcgaagat cggcgcgcga
      901 tatggcgcct cggtgaaggt ggtcgaggtg ccgcccggtc caccggtgat ggcgccgatc
      961 gtggccgaag tctatggtcc ggactatgcc cagttgcgca agatcgcgct cgggctggaa
     1021 aaggattttc attccgatcc ggacctcgtc gacgtcgaca ccagtgtcga ggccgattcg
     1081 ccgcgcgacg tggtcattgt cgaccgcgct cgcgccgcgc gcctgggcgt gagccagtcc
     1141 gacatcgcgc gcacgctcgc cgccgcgttg tccggagacg acgctaccta cctgatggac
     1201 ggacgctcga aatacgcggt accgctgcgc ctgcgcctgc cggccggcgc cc
//
LOCUS       DASRVO010000708         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_281212, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000708 DASRVO010000000
VERSION     DASRVO010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281212"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFF05_08790"
     CDS             complement(<1..210)
                     /locus_tag="VFF05_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51945.1"
                     /translation="MPQNLLAGESVRALLILVLLLPGLTLPGLLCAASIQGHLSLMVD
                     GKSLRNEEAQDAIVYFRPKVAEHVLP"
     gene            259..534
                     /locus_tag="VFF05_08795"
     CDS             259..534
                     /locus_tag="VFF05_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HZW51946.1"
                     /translation="MSQIDIRRKHSKSLKAARTAVEKTADAIGRKFAITSAWSEDTLT
                     FSRGGVTGRIRVSKTEVHVYAELGFLLGTLKPMIEAEIHRQLDENFG"
     gene            586..1764
                     /locus_tag="VFF05_08800"
     CDS             586..1764
                     /locus_tag="VFF05_08800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HZW51947.1"
                     /translation="MMSSSVVIAGAKRTAIGSMLGQFTGVPSTTLGATAIKAALDQSG
                     IAPAEVSEVIMGCVLPANLGQAPARQASLAAGLPKSTGCTTINKVCGSGMKAIMLGSD
                     LIKAGSATVVVAGGMESMTNAPHMVAARTGLRYGEAKLVDHMAWDGLTNPYDGQSMGV
                     FAEKCADKYGFTREAQDAFAIESVKRAQAAIASGAFAAEIVPVKVAGKKGEVEYATDE
                     QPGKSDIAKVPTLKPAFRKDGTVTAASSSSISDGAAATVLLDEETAKKFGIKPLARIV
                     AHATQSQEPEWFTTAPVGAIEQVLKKAGWKVADVDLFEVNEAFAVVAMAPMKDLGIDH
                     AKLNVNGGACALGHPIGCSGARLVVTLLSALKARGGKRGVASLCIGGGEATALAIELV
                     "
     gene            1859..>2091
                     /locus_tag="VFF05_08805"
     CDS             1859..>2091
                     /locus_tag="VFF05_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51948.1"
                     /translation="MQFKHAFIVAALALTLGACGDNAQQAAQDAAAKAKEAAAATAQK
                     AQETAAAAQQAASQATQAAEQAKSGDMSGAAASA"
BASE COUNT          432 a          712 c          639 g          308 t
ORIGIN      
        1 cggcaggacg tgctcggcaa ccttcgggcg gaaatacacg atcgcgtcct gcgcctcttc
       61 gttgcgcagc gacttgccat cgaccatcag cgacaagtgg ccctggatgc tggccgcgca
      121 caacagaccc ggcaacgtca ggccgggcaa cagcagcacg agaatgagca aggcacgcac
      181 gctttccccc gcgagcagat tttgcggcat catagcaaaa gcgtggcgcc tgcccgcacc
      241 cacaaaatcc cggaccccat gtcgcaaatc gatatccgcc gcaagcacag caaatcgctg
      301 aaagccgcca ggacggccgt ggaaaaaacc gcggacgcca tcggcaggaa attcgcgatc
      361 accagcgcat ggagcgagga cacgctcacc ttttcgcgcg gcggcgtcac agggcgcatc
      421 cgcgtgagca agaccgaagt ccatgtctat gccgaactgg ggttcctgct cggcacgctg
      481 aaacccatga tcgaggcgga aatccaccgc caactggacg aaaacttcgg ttgatcccgg
      541 catggaccta taatcgccga tccattcacc gattcctgcg gagccatcat gtcctcatcc
      601 gtcgtcatcg ccggcgccaa gcgcaccgcc atcggttcca tgctcggcca gttcaccggc
      661 gtgccgtcca ccacgctcgg cgcgaccgct atcaaggccg cgctggatca gtccggcatc
      721 gctccggcgg aagtctccga agtcatcatg ggctgcgtgc tgccggccaa tctcggccag
      781 gcgcccgcgc gccaggcatc gctcgcagcg ggcctgccga agtctaccgg atgcacgacg
      841 atcaacaagg tctgcggttc gggcatgaag gcgatcatgc tcggcagcga cctgatcaag
      901 gcgggctccg cgaccgtcgt cgtcgctggc ggcatggagt cgatgaccaa cgcaccacat
      961 atggtcgcgg cgcgcaccgg ccttcgctac ggcgaggcca agctcgtcga ccacatggcc
     1021 tgggacggtc tgaccaaccc gtacgacggc cagtcaatgg gtgtgttcgc ggaaaaatgc
     1081 gcggacaagt acggcttcac gcgcgaggcg caggacgcct tcgcgatcga atccgtcaag
     1141 cgcgcccagg ccgcgatcgc gtccggcgca ttcgccgccg agatcgtgcc ggtgaaggtg
     1201 gccggcaaga aaggcgaggt ggaatacgcc accgacgaac agccgggcaa gtccgacatc
     1261 gccaaggtac cgacgctgaa acccgccttc cgcaaggacg gcaccgtcac cgccgcaagt
     1321 tcttcgagca tttccgacgg cgcggccgcc accgtgctgc tggacgaaga aaccgcgaag
     1381 aaattcggca tcaagccgct cgcacgcatc gtcgcccacg ccacgcagtc acaggaaccg
     1441 gaatggttca ccaccgcgcc ggtcggcgcg atcgagcagg tgctcaagaa ggctggctgg
     1501 aaagtcgccg acgtcgacct gttcgaggtc aacgaagcgt ttgcggtggt cgctatggcg
     1561 ccaatgaagg acttgggcat cgatcacgcc aagctcaacg tcaatggcgg cgcctgcgcg
     1621 ctcggccatc cgatcggctg ctccggcgcc cgcctcgtcg taacgctgct cagcgcgctc
     1681 aaggcacgcg gcggcaagcg cggcgtcgcg tctttgtgca tcggcggcgg cgaagccacc
     1741 gcgctcgcga ttgaattggt gtaagctcga caagcccaaa aaccgacttc atcggtgggc
     1801 ggatgctgta tttccgttca ttctcaacca cacgaatatt ctcgataggg agacattcat
     1861 gcaattcaaa cacgcattta tcgttgccgc cctcgccctg actctgggcg cctgcggcga
     1921 caacgcacaa caagccgcgc aggacgccgc cgccaaggcg aaggaagccg ctgccgcaac
     1981 cgcgcagaag gcgcaggaga ctgccgcagc cgcgcagcag gccgccagcc aggcgacgca
     2041 ggccgccgag caggccaagt ctggcgacat gagcggcgcc gccgcttcgg c
//
LOCUS       DASRVO010000709         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_20779, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000709 DASRVO010000000
VERSION     DASRVO010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20779"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFF05_08810"
     CDS             <1..860
                     /locus_tag="VFF05_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZW51949.1"
                     /translation="TPAARVLPAAAADALANFAVAANARVQGPKAVAYLDSIRRSALV
                     RELMAGRQAMTWAPTRLLSDDPKKGLAHEKDTDLMVMRLARTLGGVERELYVISPYFV
                     PTSRGTGFFCSRAREGLRVAVLTNALEATDVLAVHSGYAKRRHKLLRAGVKLYELRRH
                     SPARRRWWRAQGFHGSSASSLHAKTFSVDRRRLFIGSFNFDQRSVRLNTEMGFVIDSA
                     EMATASADEFVQGVLANAYEVRLHGHRLEWVERNEGVEIVHRREPGSTFWQRLFVWIV
                     GKLPIDWLL"
     gene            978..2102
                     /gene="carA"
                     /locus_tag="VFF05_08815"
     CDS             978..2102
                     /gene="carA"
                     /locus_tag="VFF05_08815"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZW51950.1"
                     /translation="MTPAILALADGSIFCGNSIGADGQTVGEVVFNTAMTGYQEILTD
                     PSYAQQIVTLTYPHIGNTGCNAPDEESGKVQAAGLVIRDLPRMASNWRSTESLPDYLR
                     RHDIVAIAEVDTRRLTRILRETGAQNGCVMAGEGVCAQTALAAARAFSGLNGMDLAKV
                     VCTKKKYTFVEGSFDLDSARFAKPACKYRVAAYDFGVKRNILRLLADRGCEVTVVPAQ
                     TPASEVFAMNPDGVFLSNGPGDPAACDYAIAATREFIAKKIPLFGICLGHQIMGLALG
                     AKTLKMKFGHHGANHPVKDHDDGRVLVTSQNHGFAVDAATLPKNTRVTHTSLFDGSLQ
                     GFALTDAPAFCFQGHPEASPGPHDIGYLFDRFAGLMDEAR"
     gene            2104..>2853
                     /gene="carB"
                     /locus_tag="VFF05_08820"
     CDS             2104..>2853
                     /gene="carB"
                     /locus_tag="VFF05_08820"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004085673.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HZW51951.1"
                     /translation="MPKRTDIQTILIIGAGPIVIGQACEFDYSGAQACKALKQEGYRV
                     VLVNSNPATIMTDPEMADAVYIEPINRATVERIIAKEKPDALLPTMGGQTALNCALDL
                     ADCGILEKYNVELIGASREAIRMAEDRELFRRAMAEIGLECPKAEVAKSFEQALETQV
                     KVGFPTVIRPSFTLGGSGGGIAWNREEFDEIVKRGLELSPVHEVLVEESVLGWKEFEM
                     EVVRDTADNCIIVCSIENLDPMGVHTGDSITV"
BASE COUNT          535 a          894 c          918 g          506 t
ORIGIN      
        1 tgactcccgc cgcgcgcgtg ctgcctgccg cggctgccga tgcgttggcg aatttcgccg
       61 tggccgcgaa tgcgcgcgtg caggggccga aggcggtggc gtacctggat tcgatccggc
      121 gttcggcgct ggtgcgcgaa cttatggcgg ggcggcaggc catgacctgg gcgccgacgc
      181 gcctgctcag cgacgacccg aagaagggac tggcgcacga aaaggacacc gatctgatgg
      241 tgatgcgcct ggcgcgcacc cttggcggcg tcgaacgcga gctgtacgtg atttcgccct
      301 acttcgtgcc gacgtcgcgc ggtacgggtt ttttttgcag tcgcgcgcgc gaaggcttgc
      361 gcgtcgccgt gctgaccaat gcgctcgaag ccaccgatgt gctcgccgtg cactccggtt
      421 acgccaagcg ccgccacaag ctgctgcgcg ccggggtgaa gctgtacgaa ctgcgccgcc
      481 attcgccggc gcgacgacgt tggtggcgcg cgcagggctt ccatggcagc tcggcgtcga
      541 gcctgcatgc aaaaacattt tcggtggatc gccggcgcct gttcatcggc tcgttcaatt
      601 tcgaccagcg ctcggtacgt ctgaacaccg agatgggatt cgtgatcgac agcgccgaaa
      661 tggcgacggc cagtgccgac gaattcgtgc agggcgttct ggcaaacgcc tatgaggtga
      721 ggttgcacgg ccaccgcctg gaatgggtcg agcgcaacga gggcgtggaa atcgtgcacc
      781 ggcgcgagcc gggcagcacg ttctggcagc gcctgttcgt ctggatcgtc ggcaaactgc
      841 cgattgactg gctgttgtga tggacgcgac gtcgaatcac cggtaaaatt cccgctcgcc
      901 cgttgtatcg atccccggac cggcttgtgc cagcccgcgg gttctcgtgt acccgaagtt
      961 ttcaggcgaa acccctaatg acaccagcca ttcttgctct tgcagacggc agcatcttct
     1021 gcggcaattc gatcggcgcg gatgggcaaa ccgttggcga agtcgttttc aacacggcga
     1081 tgacggggta tcaggaaatc ctcaccgatc cgtcgtatgc gcaacagatc gtcaccctga
     1141 cgtatccgca catcggcaac accggctgca atgcgcccga cgaagaatcc ggcaaagtgc
     1201 aggcggcggg attggtgatc cgcgatctgc cgcgcatggc cagcaactgg cgcagcacgg
     1261 aatccttgcc cgattacctg cgtcgccacg acatcgtcgc gatagccgaa gtcgacacgc
     1321 gccggctcac gcgcatcctg cgcgaaaccg gtgcgcagaa cggctgcgtg atggcgggcg
     1381 agggcgtttg tgcacaaacc gcgctcgcgg ccgcgcgtgc attctccggc ctgaacggca
     1441 tggacctggc caaggtcgtg tgcacgaaga aaaagtatac gtttgtcgaa ggcagcttcg
     1501 atctcgacag cgcccggttc gcgaaacccg cgtgcaagta tcgcgtcgcc gcctacgatt
     1561 tcggcgtcaa gcgcaacatc ctgcgcctgc tcgccgaccg cggctgcgaa gtcacggtgg
     1621 ttccggcaca gacgccggca tcggaagttt tcgcgatgaa tcccgatggc gtgttcctgt
     1681 ccaacggccc cggcgatccg gccgcctgcg attacgcgat cgccgcgaca cgtgaattca
     1741 tcgcgaagaa gattccgctg ttcggcattt gtctgggcca ccagatcatg ggcctcgcgc
     1801 tcggcgcgaa gacgctgaag atgaaattcg gccaccacgg cgcgaatcat cctgtaaaag
     1861 atcatgatga cggccgcgtg ctggtgacct cgcaaaatca cggcttcgcg gtcgacgcgg
     1921 cgacgctgcc gaagaacacg cgcgttacgc acacgtcgct gttcgacggc tcgctgcaag
     1981 gctttgcctt gaccgacgca ccagcgttct gcttccaggg ccacccggaa gcatcgcctg
     2041 ggccgcacga tatcggctat ttgttcgacc ggtttgccgg gctcatggac gaggcccgct
     2101 gatatgccaa agcgtaccga catccaaacc atcctcatca tcggcgccgg gcccatcgtc
     2161 atcggccagg cctgcgaatt cgattactcg ggcgcgcagg cgtgcaaggc gctcaagcag
     2221 gaaggctacc gcgtggtgct ggtgaattcg aatccggcca cgatcatgac cgatccggaa
     2281 atggccgacg cggtctacat cgagccgatc aaccgcgcca cggtcgagcg catcatcgcc
     2341 aaggaaaaac ccgacgcgct gctgccgacg atgggcgggc agaccgcgct gaattgcgcg
     2401 ctcgacctgg ccgattgtgg aattctggaa aagtacaacg tcgagctgat cggcgcctcg
     2461 cgcgaggcga tccgcatggc cgaagaccgc gagctgttcc gccgcgccat ggccgagatt
     2521 ggcctcgaat gtccgaaagc cgaggtcgcc aaatctttcg agcaggcgct ggagacacag
     2581 gtaaaggtcg gattcccgac ggtgatccgc ccgagcttca cgcttggcgg cagcggcggc
     2641 ggcatcgcct ggaaccgcga ggagttcgac gaaatcgtca agcgcggcct ggaactctcg
     2701 ccggtgcacg aggtactggt cgaggaatcc gtgctgggct ggaaagagtt cgagatggaa
     2761 gtcgtgcgcg acacggccga caactgcatc atcgtgtgct cgatcgagaa cctcgatccg
     2821 atgggcgtgc ataccggcga ctcgatcacc gtg
//
LOCUS       DASRVO010000710         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_124982, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000710 DASRVO010000000
VERSION     DASRVO010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124982"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFF05_08825"
     CDS             complement(<1..201)
                     /locus_tag="VFF05_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HZW51952.1"
                     /translation="MMDVKSYMQTVGKQAREASRAIARADTNQKNRALLAMAAAIRRD
                     AAKLLEANARDMAQARANGLDTA"
     gene            complement(198..>1091)
                     /gene="holA"
                     /locus_tag="VFF05_08830"
     CDS             complement(198..>1091)
                     /gene="holA"
                     /locus_tag="VFF05_08830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZW51953.1"
                     /translation="HSERNVYTVQGRYNWQSIFASGDNFSLFAERRLTEIRIPSGKPG
                     VDGAKALEAYASKLPNDTLTLISLPGLDWKTMQSRWFAALANQGVVVEAKPVDRAALP
                     GWIDRRLAKQGLRADRAALEFLADQVEGNLLAAQQEIDKLALLLPPGNVALADVEHAV
                     VDVSRLEADALADALYAGDGARFAQVVADLRDSGEAVPAILWQVASAVTLLLRLKLAV
                     AQGDSLPAVMRTLWGRDKQRAPQIERALKRLGLAQVESALADLALIDRQAKGLERVGD
                     AWDTLLRVGMTLAAGPGNRTR"
BASE COUNT          152 a          387 c          352 g          200 t
ORIGIN      
        1 cgcggtgtcg agtccgttgg cgcgcgcctg cgccatgtcg cgcgcattgg cttccagcag
       61 cttggccgca tcgcggcgga tcgccgcggc catcgccagc agcgcacggt tcttctggtt
      121 ggtgtcggcg cgggcaatcg cacgcgaggc ttcgcgcgcc tgtttgccca ccgtctgcat
      181 gtagcttttc acgtccatca tctcgtcctg ttgcctggcc cggcggccag cgtcatgccc
      241 acgcgcagca gcgtatccca cgcatcgccc acgcgttcca ggccttttgc ctggcggtcg
      301 atcagcgcca ggtcggccaa tgcagattcg acctgcgcca ggcccagtcg tttcagcgcc
      361 cgctcgatct gcggtgcgcg ctgcttgtcg cgtccccaca gcgtgcgcat cacggcgggc
      421 aggctgtcgc cctgcgccac cgccaatttt agcctgagca gcaaagtgac cgccgacgcc
      481 acctgccaca ggatggccgg caccgcctcg ccgctgtcgc gcagatcggc caccacctgg
      541 gcgaagcgcg cgccgtcgcc ggcatacagc gcgtccgcca gtgcatcggc ctccagccgg
      601 ctgacgtcga ccacggcatg ctcgacgtcg gccagcgcca cgtttcccgg cggcagcaac
      661 agcgcgagct tgtcgatttc ctgctgtgcc gccaataaat tcccttccac ttgatcggcc
      721 aggaattcca gcgcggcgcg atcggcgcgc aaaccctgct tggccagccg gcggtcgatc
      781 cagccgggca gggccgcgcg gtcgaccggc ttcgcttcaa ccaccacgcc ctgattggcg
      841 agcgcggcga accagcggct ttgcatggtt ttccagtcga gtccgggcag gctgatgagg
      901 gtgagcgtat cgttcggcag cttgctggca taggcttcca gcgccttggc gccgtcgacg
      961 ccgggcttgc cggagggaat gcggatttcg gtcaggcggc gttcggcgaa cagcgagaag
     1021 ttgtcgccgc tggcaaaaat gctctgccag ttgtagcgcc cctgcacggt gtagacgttg
     1081 cgctcgctgt g
//
LOCUS       DASRVO010000711         1676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_281263, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000711 DASRVO010000000
VERSION     DASRVO010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281263"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="VFF05_08835"
     CDS             complement(<1..1521)
                     /locus_tag="VFF05_08835"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZW51954.1"
                     /translation="MAAGASAMGNGYIQIPGYEILRPLGAGGMSTVYLALQRSLDRTV
                     AIKVMRRGVDAAAADTAQSEKRFLLEGRMMAKLPHRNIVAVYDIVSNEDVAYIAMEFL
                     GGGALTDRMRTGIALADAIAVIVQIAGALEFAHEHGVVHRDLKPANIMFRDTGTPVLT
                     DFGIARFQDGSATRLTQTGMLVGTPTYMSPEQINGLQVDGRADQYSLGILFYELLTGA
                     PPFRGDTPIAVLMAHLTQAPAPLPPEFKAFQDIFDRVLAKNRDERYPTLKAFSDDLKS
                     RLVHSDTLLMRLQLDPNQTSSEQLRALGFYTSTPSSGGLRAVLSGQHPIPTMPPAATP
                     APGAVAAPVAPSQRKWWPVAAAIGAMLLLVVLWGLFGHRRLSHDEQELVNLWNDRAAE
                     LIKSGQLVVAAPDSDGSALDFVRKVEQKDPGNSRAEEARTQIGKLLAGQGDAALADKR
                     IDDAEKIDADALKALPGDASLVGLQQRIAQARERARAQAQADAQGAETRRKAQAQVT"
     gene            complement(1559..>1676)
                     /locus_tag="VFF05_08840"
     CDS             complement(1559..>1676)
                     /locus_tag="VFF05_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW51955.1"
                     /translation="TGLGLALAREITEAHGGRIALANRDGGGLRVSLILPNA"
BASE COUNT          248 a          554 c          589 g          285 t
ORIGIN      
        1 cgtcacctgt gcctgtgcct tgcgccgcgt ctccgcgccc tgcgcgtcgg cctgcgcctg
       61 cgcacgcgcc cgctcgcgcg cctgtgcgat gcgctgttgc aagccgacca gggatgcgtc
      121 gcccggcagc gccttgagcg catcggcatc gatcttttcc gcatcgtcga tgcgcttgtc
      181 ggccagcgcc gcgtcgccct gcccggccag cagcttgccg atctgcgtgc gcgcttcttc
      241 ggcgcgggaa ttgcccggat ccttttgttc gaccttgcgc acgaaatcca gcgcgctgcc
      301 gtcgctgtcg ggcgcggcca cgacgagctg cccggacttg atcaactccg cggcgcggtc
      361 gttccacagg ttcaccagtt cctgctcgtc gtggctcagg cggcgatggc cgaacaaacc
      421 ccacaacacg acgagcagga gcatggcgcc gatcgcggcg gcaacaggcc accacttgcg
      481 ttggctcggc gcaacgggcg cggcaactgc tccgggcgcg ggcgtcgcag cagggggcat
      541 ggtcggaatg ggatgttggc cggacaggac cgcgcgcagg ccgccgctgc tcggcgtgct
      601 ggtgtaaaag ccgagcgcgc gcaattgttc ggacgaggtc tgattcggat cgagctgcag
      661 gcgcatcagc aaggtgtcgc tgtgcacgag gcgcgatttg aggtcgtcgc tgaaagcctt
      721 gagcgtggga tagcgctcat cgcgattctt ggcgagtacg cggtcgaaga tgtcctggaa
      781 cgccttgaac tcgggcggca gcggcgccgg cgcctgggtc aggtgcgcca tcagcaccgc
      841 gatcggcgtg tcgccgcgga acggcggcgc gccggtgagc agctcataga aaagtatgcc
      901 gaggctgtac tggtcggcgc ggccgtcgac ttgcaggccg ttgatctgct ccgggctcat
      961 gtaggtgggc gtgcccacga gcatgccggt ctgggtcagg cgcgtggccg aaccgtcctg
     1021 gaagcgggcg atgccgaagt cggtcagcac cggcgtaccg gtatcgcgga acatgatgtt
     1081 ggcgggcttc aggtcgcggt gcacgacgcc gtgttcgtgc gcgaactcca gcgcgccggc
     1141 gatctgcacg atcaccgcga tcgcatcggc cagcgcgatg ccggtgcgca tgcggtcggt
     1201 gagcgcacca ccgcccagga attccatcgc gatgtaagcg acgtcctcgt tgctgacgat
     1261 gtcatacacc gcgacgatat tgcggtgcgg cagcttggcc atcatgcggc cttcgaggag
     1321 gaagcgcttt tccgattgcg cggtgtcggc cgcggccgcg tccacgccgc ggcgcatgac
     1381 cttaatggcc acggtgcggt ccagcgagcg ttgcagagcc aggtacacgg tcgacatgcc
     1441 gcccgcccca agcggacgca ggatctcgta gccgggaatc tggatgtagc cgttgcccat
     1501 cgcgctagcc cccgcagcca tcatagcatc ggactgcgca ggggaaacgg cgcgtgggct
     1561 aggcgttcgg caggatcagg ctgacccgca gcccgccgcc atcgcggttg gccagcgcga
     1621 tgcggccgcc gtgcgcctcg gttatttcgc gcgccagcgc caggcccagt cccgtg
//
LOCUS       DASRVO010000712         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_98184, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000712 DASRVO010000000
VERSION     DASRVO010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98184"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..>1003
                     /gene="ccsA"
                     /locus_tag="VFF05_08845"
     CDS             293..>1003
                     /gene="ccsA"
                     /locus_tag="VFF05_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZW51956.1"
                     /translation="MIALAHAVAIVLYLAAAGLAAIPFARPIRAPVRSVIWVLAVGIA
                     VHVVALVDFVRIHGEPPLTGLGPAMSFAAVLVAITLLTVEALAHEVSLTLVAAPLAAL
                     LTGFASLAGMVPGPEQNGMRGVWLTSHIALSFLGIAAFATAAAAGAMYLVERRELKSR
                     HFGAIFRFFPPLETLDRVNHAAVVAGWLALTVGIGLASSYALEYRTAHAIKMVWAYSA
                     WLAVTALTIGRVVRGWCAH"
BASE COUNT          139 a          329 c          338 g          197 t
ORIGIN      
        1 gctgccggaa gagcccatcg ccctggcgca ggcggtgaag gtaaccgatc gcggcacgac
       61 cgcgatcatt ctggaccagc gtcaaccgtc gatcacgtcg ggcacgccgg cgcgtctcac
      121 ggcgcggatg ccgtgacgga tcacctcgcc gcgctcgagc gcggcgggct cacgcgatga
      181 acggtaacgg ggaaggcgaa cgcatgcgcc ttccccgtta ccgtttcttg cgtaagaaag
      241 ttcggaggcc catgtacgga tctgcctgat cctcagtaga ttacgctccg acatgatcgc
      301 cctcgcgcat gccgtcgcca ttgtgttgta tctcgctgct gcgggattgg cggccatccc
      361 ctttgcgcgc ccgattcgcg ccccagtgcg cagcgtgatc tgggttctgg cggttggaat
      421 cgcggttcat gtcgtggcgc tcgtcgattt cgtgcgcatc cacggggagc cgccgctcac
      481 cggtctcggg ccggcgatgt cgtttgcggc cgtgctcgtt gcgattacgt tgttgaccgt
      541 cgaagcgctc gcgcacgagg ttagtctcac actggtcgcg gcgccgttgg ccgcgctact
      601 cactggtttc gccagccttg caggcatggt gcctggtccc gaacagaacg gcatgcgcgg
      661 cgtgtggctc acgtcgcaca tcgcgctcag cttcctcggc atcgcggcgt tcgcaacggc
      721 ggccgccgcc ggagccatgt atctcgtgga acgccgcgag ctcaagtcgc ggcatttcgg
      781 cgcgatcttc cgcttcttcc cgccgctcga gacgctcgat cgcgtcaacc acgcggctgt
      841 cgtggccgga tggctggcgc tcacggtcgg gatcgggctg gcgtcgagct acgcgttgga
      901 ataccgcacg gctcacgcga tcaagatggt atgggcgtac agcgcgtggc tcgcggtgac
      961 tgcgctgacg atcggccgcg tggtgcgcgg ctggtgcgcg cac
//
LOCUS       DASRVO010000713         1056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_47679, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000713 DASRVO010000000
VERSION     DASRVO010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47679"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..>1056
                     /locus_tag="VFF05_08850"
     CDS             42..>1056
                     /locus_tag="VFF05_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51957.1"
                     /translation="MKPAEVAAAKPGEAATAKEAPKLTAKDIWGDISASEKPGEAKPG
                     ATPAPATATPSLAAKAPESTTPATVAATTPAPAATAPEKAAAAKPAETKPASTPVAVK
                     PKAPAKVEPISAPGLPWWWNNNVLMASGAALLIIGLLVLMRFMRKPKADLVPAAGMPA
                     AHDEHLDEMAADEQHLLDQLAQHPGDAALSLQLLDLYYAHQDSAKFEAAAEAMYAHIA
                     DPTSAEWQQVRAMGEHLCPHNPLFGGHEDLAASLHDMHTATEHAEHDHVHMDDTHADH
                     FDAGEFHTQEMPRPAEENFDFDLADHAASVQVEPAHAEPAYVPPPAPVHAEPAYVPPP
                     APVH"
BASE COUNT          208 a          368 c          334 g          146 t
ORIGIN      
        1 ctcctgcgcc tgcgaaaccc gccgaggcca agcctgtcga aatgaaacct gccgaagtgg
       61 cggctgccaa gcccggcgaa gcggcgacgg cgaaggaagc gccgaagctg accgccaagg
      121 acatctgggg cgacatcagc gccagcgaaa agccgggcga agcgaaaccg ggtgcgacgc
      181 ctgctccggc cactgccacg ccgtcgcttg ccgccaaggc tccggaatcc accacgccgg
      241 caacggtcgc ggcgacgacg cctgcgccag cggctaccgc tcccgagaag gcagccgcag
      301 ccaagcccgc ggaaacgaaa ccggcatcca cgccggtcgc ggtgaagccg aaagcgccgg
      361 cgaaggtcga gccgatttcc gcgccgggat tgccgtggtg gtggaacaac aatgtgctga
      421 tggcgagcgg cgccgcactg ctcatcatcg gactgctcgt tctgatgcgc ttcatgcgca
      481 agcccaaggc tgacctcgta cccgccgccg gtatgccggc agcgcacgat gaacacctgg
      541 atgagatggc cgccgacgag cagcatctgc tcgatcaatt ggcccagcat ccgggggatg
      601 cggccttgtc gttgcagttg ctggatctgt attacgcgca tcaggattcc gccaaattcg
      661 aggcggccgc cgaagccatg tatgcgcaca tcgccgaccc gacttcggcc gagtggcagc
      721 aggtgcgcgc gatgggagag catctgtgtc cgcacaatcc gctgttcggc ggccacgagg
      781 atctggccgc gtccctgcac gacatgcaca cggccaccga gcacgccgaa cacgatcacg
      841 tgcacatgga cgacacgcat gccgatcact tcgacgcggg tgaattccat acccaggaaa
      901 tgcccaggcc cgccgaggaa aatttcgatt tcgatctggc cgatcacgcc gcttccgtgc
      961 aagtcgagcc tgcgcatgcg gaaccggcct acgtgccgcc gccggcgccc gtccacgcgg
     1021 aaccggccta tgtgccaccg ccggcgcccg tccacg
//
LOCUS       DASRVO010000714         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_384344, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000714 DASRVO010000000
VERSION     DASRVO010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384344"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VFF05_08855"
     CDS             <1..1123
                     /locus_tag="VFF05_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZW51958.1"
                     /translation="SQMLRDETAASYEPFVHAEDLPTFRRDLVAHLKGETSFLESTYR
                     TPDMNDQWRWMLTRGRVVERDARGLALRMVGTTQDITTLKEAEESLRKLNEELESRVD
                     SRTADFRRANIELRQTLEQLTQTQRQLLESEKMAALGGLVAGVAHEINTPLGVTVTAA
                     SHLREEAMRLARQPMLDPEDLIGFHAMIGESSEIILRNLQRADRLIKSFKLVAVDQTT
                     EERRRIELGAYLNDILVSLGPSLKKTPHVVAIECQEPVWLDTYPGALYQIVSNLVMNS
                     LHHAFAPEQAGTITVTARQNENVVELTYRDNGVGMSDAVRARVFEPFFTTRRGQGGSG
                     LGLHVVYNLVTQLLKGSIRVDSAPGVGAVFEIFLPCNSG"
     gene            complement(1127..>1705)
                     /locus_tag="VFF05_08860"
     CDS             complement(1127..>1705)
                     /locus_tag="VFF05_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZW51959.1"
                     /translation="EMVHLLAGAAETRSWETAAHVKRVGMIAELLGQFYGLDEKTTNA
                     LRTASPLHDIGKVGIPDAVLNKPGAHTPEEMTIMRTHAELGARLLSMSQRPLLQLAAE
                     IAMTHHENWDGTGYPRGLSGEAIPIGGRITMLADVFDALGSRRCYKEPWSAEDIRAFI
                     VEQSGVKFEPRLVELLFENWDRVQSVRGQLPD"
BASE COUNT          317 a          537 c          545 g          306 t
ORIGIN      
        1 ttcgcaaatg ctgcgcgacg aaaccgcggc atcctacgaa cctttcgtgc atgcggaaga
       61 tctgccgacg ttccggcgcg acctggtcgc ccacctgaaa ggcgaaacca gtttcctcga
      121 atccacctat cgcaccccgg acatgaacga ccagtggcgc tggatgctga cgcgcgggcg
      181 cgtggtcgag cgcgatgcgc gcggactcgc gctgcgcatg gtcggcacca cccaggacat
      241 caccacgctc aaggaggccg aggaatcgct gcgcaagttg aacgaggaac tcgaatcgcg
      301 cgtggattcg cgcaccgcag attttcgccg cgcgaatatc gagctgcggc aaacgctgga
      361 gcaattgacg cagacgcaac gccaactgct ggaatcggaa aagatggcgg cactcggcgg
      421 cctcgtggcc ggcgtcgccc acgagatcaa cacgccgctg ggcgtgaccg taaccgcggc
      481 atcgcacctg cgcgaggagg cgatgcgcct ggcacggcag ccgatgctcg atccggagga
      541 ccttatcggc ttccacgcaa tgatcggcga aagctcggaa atcatcttgc gcaacctgca
      601 gcgcgccgac cgcctgatca agagtttcaa gctcgtcgcg gtcgaccaga ccacggaaga
      661 acggcgccgc atcgaacttg gcgcctacct gaacgatata ctggtctcgc tcggcccgag
      721 cctgaaaaag acgccgcatg tcgtcgcaat cgagtgccag gaaccggttt ggctcgacac
      781 gtatcctggc gcgctttacc agatcgtgag caatctggtg atgaattcgc tgcatcacgc
      841 gttcgcgccg gaacaagccg gtacgatcac cgtcaccgcc cgccagaacg aaaacgtggt
      901 cgaattgacc taccgcgaca acggcgtcgg catgagcgat gccgtgcgtg cgcgcgtctt
      961 cgagccgttt ttcaccacgc gtcgcggaca gggcgggtcc gggctcggtc tgcacgtggt
     1021 ttacaacctg gtgacgcagc tgctcaaggg cagcatccgt gtcgacagtg cgcccggcgt
     1081 cggcgcggtg ttcgagattt tcctgccgtg caattccggc tagggttcaa tccggcagtt
     1141 gcccgcgtac ggactgcacc cgatcccagt tctcgaacag caattcgacc aggcgcggtt
     1201 cgaatttcac gccgctttgt tcgacgatga aggcacggat gtcttcggcc gaccacggct
     1261 ccttgtaaca acggcggctg ccgagcgcat cgaagacgtc ggcaagcatg gtgatgcggc
     1321 cgccgatggg gatggcttcg ccgctgaggc cgcgggggta accggtgccg tcccagtttt
     1381 cgtggtgcgt catcgcgatt tcggcggcta gctgcaacag cggacgttgc gacatcgaca
     1441 acaggcgtgc gccgagttcg gcgtgcgtgc gcatgatcgt catttcctcc ggcgtgtgcg
     1501 cgcccggctt gttgagcacg gcgtcgggga tgccgacctt gccgatatcg tgcagtggcg
     1561 atgcggtgcg caaggcgttg gtggtcttct catccagtcc gtagaactgg ccgagcagtt
     1621 cggcgatcat gccgacgcgt ttgacgtggg ccgcggtttc ccacgaccgc gtctcggcgg
     1681 cgccggcgag cagatgcacc atttc
//
LOCUS       DASRVO010000715         1024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_73089, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000715 DASRVO010000000
VERSION     DASRVO010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73089"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /gene="priA"
                     /locus_tag="VFF05_08865"
     CDS             <1..963
                     /gene="priA"
                     /locus_tag="VFF05_08865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZW51960.1"
                     /translation="VTERLRRGEQTMLLLNRRGYSPVVLCRTCGDTIQCKHCAISLTH
                     HKRERRLECHYCGFTQPVPERCPKCGSDYVQFLGTGSERLEELLHQTFPQARIARLDR
                     DTVRGRDDFERILAALHAGEIDVLVGTQMIAKGHDIHGVTLVGVVGADTGLSMPDFRA
                     AERTFQLLTQVAGRAGRGDAPGEVIVQTWFPDHYAVQFAARHDYQGFYDKEARYRSWM
                     KYPPFSALANFLVRSDSLDAAMNWSGTLGRWLERQPKEGIRALGPAPAPLSKLKNDYR
                     YHFVLKSQSRAKLNALLRAMLAYAAQQKIPRANVVADVDAVSLL"
BASE COUNT          185 a          337 c          331 g          171 t
ORIGIN      
        1 gtcaccgagc gcctgcgacg cggcgagcaa accatgctgc tgctcaatcg tcgcggctat
       61 tcgccggtgg tgctctgccg aacgtgcggc gacaccatcc agtgcaagca ctgcgcgatc
      121 tcgctgacgc atcacaagcg cgagcgccgt ctcgagtgcc actactgcgg ctttacgcag
      181 cccgtgccgg agcgctgccc gaagtgcggt agcgattacg tgcagtttct cggcaccggc
      241 tcggaacgcc tggaagaact gctgcaccag acgtttccgc aagcgcgcat cgcccgcctc
      301 gatcgcgata cggtgcgcgg gcgcgatgac ttcgagcgca tcctggccgc gctgcacgcc
      361 ggagagatcg acgtcctggt gggcacgcag atgatcgcca agggccacga catccacggc
      421 gtgacgctgg tgggcgtggt gggcgccgat accggcttgt cgatgcccga cttccgcgcc
      481 gccgaacgca ccttccagtt gctcacgcag gtggcgggac gcgccggacg cggcgatgca
      541 cccggcgaag tcatcgtgca gacatggttt cccgatcact acgccgtgca gtttgccgcg
      601 cgtcacgatt accaaggctt ctacgacaag gaagcgcgtt accggagctg gatgaagtat
      661 ccgcccttca gcgcgctcgc aaatttcctg gtgcgcagcg actcgctcga cgcggcgatg
      721 aactggagcg gaacgctggg gcgctggctg gagcggcaac cgaaggaggg aatccgcgcc
      781 ctcggtccgg cgccggcgcc gctctcgaag ctgaagaacg actatcgcta ccacttcgtg
      841 ctcaagtcgc agagccgcgc caaattgaac gccctgctgc gcgctatgct agcctatgcc
      901 gcgcagcaga agatcccccg cgccaacgtg gtggccgatg tggacgcggt gtcgctgctg
      961 taaacgccgc ttgcggggag tagcagcggt tcaatccaag cacgcgttag ccatttataa
     1021 aggc
//
LOCUS       DASRVO010000716         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_229201, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000716 DASRVO010000000
VERSION     DASRVO010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229201"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /gene="rlmD"
                     /locus_tag="VFF05_08870"
     CDS             <1..1234
                     /gene="rlmD"
                     /locus_tag="VFF05_08870"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014507156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZW51961.1"
                     /translation="GKAVFVTGALAGERVRAHFSGKHRHYDEAVVEEVLQASPDRVVP
                     CCAHFGVCGGCVLQHLKPEAQIAAKQRVLLENLERIGKVAPKRILPPLVDSPWGYRRK
                     ARLSVKFVDKKGRVLVGFRESNGRYVADIARCEVLMPAVGERIADIAALIETLDAKRD
                     IPQIEVAIGDDLAVLVFRHLQPLSERDRAALVEFGTKWNLGIFLQPGGIDSVAPLWPA
                     DAQLLFRLPKYDVGFEFKPLDFVQVNAGMNRAMIDHALGLLDPQPTDRVLDLFCGLGN
                     FTLPLARRAAQVTGVEGEAGLVRRAGENAQRNGVTNAQFFAADLAADQRDAPWAKADH
                     DLLLLDPPRSGAEALLEYMPKKSARRIVYVSCHPGSLARDAGTLVNRHGFTLTSAGAM
                     DMFPHTAHVESIALFDRK"
     gene            1243..2253
                     /gene="nagZ"
                     /locus_tag="VFF05_08875"
     CDS             1243..2253
                     /gene="nagZ"
                     /locus_tag="VFF05_08875"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZW51962.1"
                     /translation="MLIVGIKDKQLSPQEREWIRAPQVGGVILFTRNFESREQASELI
                     AEIRSVRAEPLLLCVDQEGGPVQRFRDGFTRLPALSRIGEVYARDRQRALELAGEHAW
                     LMASEMRAIDIDLSFAPVLDLACGNVVVGERAFHADPRIDSELGLEYVRNMRLAGMAA
                     TIKHFPGHGSVREDTHLEPAIDRRALDEIRDADMIPFADAIVAGAEAVMMGHVTYPAV
                     DALPAGYSRVWIQDILRGELGFGGVVFSDDISMAAAEDAGGIGARIAAHRDAGCDIVL
                     VCKPEIVSEALAVCRNEDPCDPAIVEAQRGRVASTWQSLVENPQRARFAARLTALETV
                     AA"
     gene            2250..2795
                     /locus_tag="VFF05_08880"
     CDS             2250..2795
                     /locus_tag="VFF05_08880"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006449878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HZW51963.1"
                     /translation="MTRPALSEALKDADLLHVRAELDKAIARMGRDIDAALAGEVPLY
                     LSVMHGGLIFSGALALAISTDLEFDYVHATRYRGETHGGELHWLRKPASALAGRTVLL
                     ADDILDEGYTLRAVRDWCIGEGARRVLIAVMCQKRHDRTAPGIRADFCGVEVPDRYVF
                     GFGMDFHEQGRNLPAIYALKE"
     gene            2797..>3262
                     /locus_tag="VFF05_08885"
     CDS             2797..>3262
                     /locus_tag="VFF05_08885"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465944.1"
                     /note="Catalyzes the phosphorolytic cleavage of
                     6-oxopurine nucleosides; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZW51964.1"
                     /translation="MSLDLAIIGGTGLYAFPGLENARREAVATPFGAPSGEVMLGTLA
                     GQRVAFLARHGEAHTLAPHRVNYRANLWALHHLGARRMVGVNAVGGIGEKMGPRVLAV
                     PDQIIDYTHGRLDSFCDVEGAKVEHIDLTEPYSAALRTGLIAAANRAGIAVVD"
BASE COUNT          552 a         1046 c         1088 g          576 t
ORIGIN      
        1 cggcaaggcc gtgttcgtga ccggggcgct ggccggtgaa cgcgtacgtg cgcatttcag
       61 cggcaaacac cggcattacg acgaggctgt ggtcgaagaa gtactgcagg cttcgcccga
      121 tcgcgttgtg ccgtgctgcg cgcatttcgg cgtttgcggt ggttgcgtgc tccagcatct
      181 gaaacccgag gcgcagatcg cggcgaaaca gcgcgtgctg ctggaaaacc tcgaacgtat
      241 cggcaaggtt gcgccgaaaa ggatcctgcc gccgctggtg gattcgccct ggggctaccg
      301 gcgcaaagcg cgcctgagcg tgaaattcgt cgacaagaag ggcagggtgc tggtcggatt
      361 ccgcgagagc aatggtcgct acgtcgccga tatcgcgcgc tgcgaggtgc tgatgccggc
      421 agtcggcgag cgcatcgccg acatcgccgc gctgatcgag acgctggatg ccaagcgtga
      481 cattccgcaa atagaggttg cgatcggcga cgacctcgcc gtgctggtgt tccggcatct
      541 gcaaccgctg tccgagcgcg accgtgcggc gctggtcgaa ttcggcacaa agtggaacct
      601 gggaatcttc ctgcagccgg gcggcatcga cagcgtcgcg ccgctgtggc cggcagatgc
      661 gcaacttttg ttccgcttgc cgaaatacga tgtcgggttc gaattcaagc cgctggattt
      721 cgtgcaggtc aacgccggca tgaatcgggc gatgatcgat cacgcgctgg gtttgctcga
      781 tccgcagccg acggatcgcg tgctggacct gttctgcggc ctcggcaatt tcaccctgcc
      841 gctggcgcgc cgcgccgcgc aagtgacggg cgtggaaggc gaggccggcc tggtgcgccg
      901 tgccggcgag aacgcgcagc gcaacggcgt gaccaacgcg caattcttcg ccgccgacct
      961 cgccgccgac cagcgcgatg cgccgtgggc caaggccgat cacgatctgc tcctgctcga
     1021 tccgccgcgc agtggtgcgg aagcgctgct ggaatacatg ccgaagaaat ccgcgcggcg
     1081 catcgtctat gtgtcgtgtc atcccggttc gctggcgcgc gatgcaggca cgctggtgaa
     1141 ccggcacgga ttcaccctga ccagcgccgg ggccatggac atgtttccgc ataccgccca
     1201 tgtagaatcc atcgcattgt tcgacaggaa ataagcattt tcatgctgat cgtcggaatc
     1261 aaggacaagc aactctcgcc acaggagcgc gagtggattc gtgcgccgca ggtcggcggc
     1321 gtgatcttgt tcacgcgcaa tttcgaatcg cgcgaacagg cgagtgagtt gatcgcggaa
     1381 attcgctccg tgcgcgcgga gccgctgctg ctctgcgtcg atcaggaagg cggcccggtg
     1441 cagcgtttcc gggacggatt cacgcgcctg cccgcactgt cgcggatcgg cgaggtttac
     1501 gcgcgcgacc ggcaacgggc actggaactg gccggcgagc atgcctggct gatggccagc
     1561 gagatgcgcg ccatcgacat cgacctgagc ttcgcgccgg tgctcgatct ggcctgcggc
     1621 aatgtcgtcg tcggcgaacg cgcgttccat gccgacccga ggatcgactc ggagcttggc
     1681 ctggaatacg tgcgcaacat gcgcctcgcg ggcatggcgg cgacgatcaa gcattttccc
     1741 ggccacggtt cggtacgcga ggacacgcat ttggaaccgg ccatcgaccg gcgcgcactc
     1801 gacgaaatcc gcgacgccga catgattccc tttgccgacg ccatcgtggc gggcgcggag
     1861 gccgtgatga tgggacacgt gacgtatccg gccgtggatg cgctacctgc cggttattcg
     1921 cgcgtctgga tccaggatat cctgcgcggc gaacttggct ttggcggcgt cgtgttcagc
     1981 gacgacatca gcatggccgc ggcggaagac gccggcggca tcggcgcgcg catcgccgcg
     2041 catcgcgacg ccggttgcga catcgtgctg gtgtgcaaac ccgagatcgt gtccgaggcg
     2101 cttgccgttt gccgcaacga agatccctgc gatcccgcca ttgtcgaagc gcagcgcggt
     2161 cgcgtggcgt cgacctggca aagtctcgtc gaaaatcccc aacgcgcgcg cttcgccgcg
     2221 cgcctcaccg cactggaaac cgtagccgca tgacacgtcc cgcactctca gaagcactca
     2281 aagacgcgga cttgctccac gtccgcgccg aactcgacaa ggccatcgca cgcatgggcc
     2341 gcgacatcga cgccgcgctg gcgggcgagg tgccgttgta tctgagcgtg atgcatggcg
     2401 gactgatttt ttccggcgcg ctcgccctgg ccatttccac cgatcttgaa ttcgactacg
     2461 tgcatgccac gcgctatcgc ggcgaaaccc atggcggcga attgcactgg ctgcgcaagc
     2521 cggcgtcggc gctggccgga cgcaccgtgc tgcttgccga cgacatcctc gacgagggct
     2581 acacgttgcg cgcggtgcgc gactggtgca tcggcgaggg tgcgcgtcgc gtgctgatcg
     2641 cggtgatgtg ccagaagcgt catgaccgca ccgcgcccgg catccgcgcc gatttctgcg
     2701 gcgtcgaggt gccggatcgc tacgtgttcg gattcggcat ggatttccac gagcagggtc
     2761 gcaacctgcc cgcaatctat gcgctgaagg aatgacgtga gcctcgatct tgccatcatc
     2821 ggcggcaccg gcctgtacgc gtttcccgga ttggaaaacg cgcggcgcga agccgtcgcc
     2881 acgccgttcg gtgcgccgtc gggcgaggtc atgctcggca cgcttgcggg ccagcgcgtc
     2941 gcttttctcg cacgtcacgg cgaagcgcac acgcttgccc cgcaccgcgt gaactaccgc
     3001 gccaatctct gggcgctgca tcatctcggc gcgcggcgca tggtcggcgt caacgcggtc
     3061 ggcggcattg gcgagaaaat gggcccgcgc gtactcgccg tgccggacca gatcatcgac
     3121 tacacccacg ggcggctgga cagcttctgt gacgtcgagg gtgcgaaggt cgaacacatc
     3181 gacctcaccg aaccttacag cgcggcattg cgcactggct tgatcgccgc ggccaaccgc
     3241 gccggtattg cggtggtcga cg
//
LOCUS       DASRVO010000717         1182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_175518, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000717 DASRVO010000000
VERSION     DASRVO010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175518"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VFF05_08890"
     CDS             complement(<1..262)
                     /locus_tag="VFF05_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51965.1"
                     /translation="MPTRLKPLLQLALAIASLVVVFDMLVFRSGVYAPLIEFESTAGS
                     VVTATRAIDTYFDPARRNVLVLGDSKVGQGFSALLADASSARP"
     gene            389..>1182
                     /locus_tag="VFF05_08895"
     CDS             389..>1182
                     /locus_tag="VFF05_08895"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HZW51966.1"
                     /translation="MVLFQSITFKVFGIAFLALLMLVPLAQVQGLIAERNGMRDSAIA
                     TIAQRWGEPQRIGGPLLYIPRRVRATTDKGVVVRDDGRDVVLPDRLKIQGVLVPEVRQ
                     YGIYATPVYTSELRITGEFLDADLGKLRGADFWLWDQAELRIPVTDVRGIRVVSPLAF
                     AGDELRLGPSGGAIGRLGAIAVPVSLVRKDGGTYAFELTMTLAGTQSLSFLPLARSTE
                     VALSSSWPDPAFDGAFLPTSRKVDANGFSAQWSVLDFNRGFGQSWR"
BASE COUNT          188 a          391 c          413 g          190 t
ORIGIN      
        1 cggggcgagc ggacgacgca tcggcgagaa gcgcggagaa tccctggccg accttcgagt
       61 cgccgagcac gagcacattg cggcgtgcgg gatcgaaata cgtatcgatc gcccgcgtcg
      121 cggtcacgac cgatcccgca gtcgattcga attcgatcag cggggcgtag acgccgctgc
      181 ggaagaccag catatcgaag acgacgacga gcgatgcgat cgccagcgcg agctgcagca
      241 ggggctttag gcgggtcggc atccgcgcgg attctgcatg gaacgtcagg ccggcgccag
      301 cgttcgcccg cctttcgcca catccggagc gcaacggcgc ccgttgccgg cgctgcaatg
      361 gcggccggca agcagatgga gcgaatcaat ggtcctgttc cagtcgataa cgttcaaggt
      421 attcgggatt gcgttcctgg cattgctgat gctggtcccg ctggcccagg tgcagggcct
      481 gatcgcggag cgcaacggca tgcgcgacag cgcgatcgcc acgatcgcgc agcgctgggg
      541 cgagccgcag cgcatcggcg gacccctgct ctacattccg cggcgggtgc gggcgacgac
      601 cgacaagggc gtcgtcgtgc gcgacgacgg ccgtgacgtc gtgctgccgg accggctgaa
      661 gatccagggt gtcctcgttc ccgaggtgcg ccaatacggc atttacgcaa cgccggtgta
      721 taccagcgag ttgcggatca ccggcgagtt tctcgacgcc gacctgggca agctgcgcgg
      781 cgccgatttc tggctctggg accaggccga actgcgcatc ccggtgaccg acgtgcgcgg
      841 cattcgcgtc gtctcgccgc tcgcgttcgc cggcgacgaa ttgcgcctgg gcccgtcggg
      901 cggagcgatc ggccgcctcg gcgcgatcgc cgttccggtc agcctcgtgc gcaaggacgg
      961 cggcacgtac gcgttcgagc tgaccatgac cctggccggg acgcagagcc tgtcgttcct
     1021 gccgctggcg cgcagcaccg aagtcgcgtt gtcgtcgtcg tggcccgacc cggcattcga
     1081 cggcgcattc ctgccgacca gccgcaaggt cgatgcgaac ggcttcagcg cccagtggtc
     1141 ggtgctcgac ttcaatcgcg gcttcggcca gtcctggcgc ga
//
LOCUS       DASRVO010000718         1006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_254477, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000718 DASRVO010000000
VERSION     DASRVO010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254477"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1006
                     /locus_tag="VFF05_08900"
     CDS             <1..>1006
                     /locus_tag="VFF05_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZW51967.1"
                     /translation="NIVHLVLARVTGAPEGVKGISLFVVPKFMVGKDGAVGAHNDVHC
                     VSIEHKLGIKASPTAVLQFGDHDGAVGYLVGQENRGLEYMFIMMNAARYAVGVQGVAI
                     AERAYQLAVEYAKERVQSRPVDGSMNASAPIIHHPDVKRMLMTMRAYTEGCRAMATVA
                     AAAYDAAHHHPDGEARKQNQAFYEFMVPLVKGFSTEMSLEVTSLGIQVHGGMGYIEET
                     GAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGQTAKPIAAQIAITESELAKLDT
                     PDARAVLKRLAAARQAFLEVVDFVAGNTKASPNAVFAGSVPYLMLAGNLMAGWQMARS
                     L"
BASE COUNT          185 a          329 c          343 g          149 t
ORIGIN      
        1 agaacatcgt gcacctggtg ctggcgcgcg tgaccggcgc gccggagggc gtgaagggca
       61 tcagcctgtt cgtcgtgccg aagttcatgg tcggcaagga cggcgccgtc ggcgcgcaca
      121 acgacgtgca ttgcgtcagc atcgagcaca aattgggcat caaggcttcg ccgaccgcgg
      181 tgctgcaatt cggcgatcac gatggggccg tgggctatct cgtcggccag gagaaccgcg
      241 gcctcgagta catgttcatc atgatgaatg ccgctcgtta cgccgtgggc gtgcagggcg
      301 tggcgatcgc cgaacgcgcc taccagctgg ccgtcgagta cgcgaaggaa cgcgtccagt
      361 cgcggccggt ggacggctcg atgaatgcga gcgcgcccat catccatcac ccggacgtga
      421 agcgcatgct gatgaccatg cgcgcgtata ccgaaggatg ccgcgcgatg gccaccgtgg
      481 cggcggcggc ctacgacgcc gcgcaccacc atccggacgg cgaggcgcgc aagcagaacc
      541 aggcgttcta cgaattcatg gtgccgctcg tgaagggctt cagcaccgaa atgagcctgg
      601 aagtgacctc gctcggcatt caggtccacg gcgggatggg ctacatcgag gaaacgggcg
      661 cggcgcagta ctaccgcgac gcgcgcatcc tgccgatcta cgaaggcacc acggcgatcc
      721 aggccaacga cctggtcggc cgcaagaccg cgcgcgacgg cgggcagacc gccaagccga
      781 tcgcggcgca gatcgcaatc accgaatcgg agctcgcgaa actggacacc cccgacgcgc
      841 gcgccgtgct gaagcgcctc gccgccgcgc gccaggcgtt cctcgaggtg gtcgattttg
      901 tcgccggcaa caccaaggct tcgcccaacg ccgtgttcgc cggatcggtg ccgtacctga
      961 tgctcgcggg caacctgatg gccgggtggc agatggctcg ttcgct
//
LOCUS       DASRVO010000719         1019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_175590, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000719 DASRVO010000000
VERSION     DASRVO010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175590"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFF05_08905"
     CDS             <1..909
                     /locus_tag="VFF05_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51968.1"
                     /translation="VMSANVDFYADDTLNAQFQVEAEEAATARSRRRDAEPALGLDDL
                     FKPDSEAAKPQFAVPMQLDPGTRIERGSRAVSARSDRRIADSVRGIRGPEGKPEGINI
                     AADDDVNANSASGNHPLRDYLHKNLLGGPARAALEGAPELVLDPKAQSFHASGGLAAL
                     AAYCKAEFGQSAWRPVTTQDLSRLRTQQPARPYAHLQWLDAFVHAAGRLARHLDPGGR
                     YRLKGAALTGADVPHHARIVAALATPAKLNEIATASGAPMAAVFDLVSAYDAIGLIEV
                     QSRLARHAPAPKSGLFLRLRNPFSKR"
BASE COUNT          194 a          358 c          314 g          153 t
ORIGIN      
        1 gtgatgagtg cgaacgtcga cttctacgcc gacgacacgc tcaacgccca attccaggtc
       61 gaagccgaag aagccgcgac cgcgcgctcg cgacgccgcg atgccgaacc cgcgctgggc
      121 ctggacgatc tgttcaaacc cgacagcgag gcggccaagc cgcaattcgc ggtgcccatg
      181 cagctcgacc caggcacgcg catcgaacgc ggcagccgcg cggtttcggc gcgcagcgat
      241 cgccgcatcg ccgactcggt gcgaggaatc cgcggaccgg aaggcaaacc cgaaggcatc
      301 aatatcgccg ccgacgacga tgtgaatgcg aattcggcat ccggcaatca tccgttgcgc
      361 gactacctgc acaagaacct gctcggtgga ccggcacggg ctgcgttgga aggcgcgccg
      421 gaactggtgc tcgatcccaa ggcccaatcc ttccacgcca gcggcggttt ggccgcgctg
      481 gcggcgtatt gcaaagccga gttcgggcaa tcggcgtggc gcccggtgac cactcaggac
      541 ttgtcccgcc tgcgcacgca acagccagca cggccctacg cgcacctgca atggctcgat
      601 gcgttcgtgc atgccgcagg ccgcctggct cggcacctgg accccggcgg tcgctaccgt
      661 ctcaagggcg ccgccctgac cggcgccgac gtcccgcatc acgcgcgcat cgtggctgca
      721 ctggccacac ccgcgaagct caacgaaata gcgactgcca gcggcgcgcc gatggctgca
      781 gtatttgacc tcgtcagcgc ctacgacgcg attggcctga tcgaggtgca aagccgcctg
      841 gcacggcatg cgcccgcacc gaaatccggt ctgtttttgc gcctgcgcaa tcccttttcc
      901 aagcgctgag gcgagatcga ccgcttgacc cggctgcgga atcggcgaca attctcggat
      961 tcacggcatc gcccagacgc gatatccgta ccaactactc aggggtagaa catggcaag
//
LOCUS       DASRVO010000720         1232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_332948, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000720 DASRVO010000000
VERSION     DASRVO010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332948"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFF05_08910"
     CDS             complement(<1..726)
                     /locus_tag="VFF05_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016851068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW51969.1"
                     /translation="MSDAHTRFSPSRREALRSIGLIAGSAAMYRAMTALGATRESKYA
                     GPVDLSGAPRGASVLILGAGLAGMTAAYELRNAGYRVQVLEYNARPGGRNWTLRGGDT
                     YTELGGARQHCEFDKGLYLNPGPWRIPYHHENLLHYCRVLGIALEPFVQVNYNAYLHG
                     EHALGGKPQRYRSYKADYQGMLAELLAKAVDQGALDKEFAKEDGEKLLESLREWGMLD
                     EKMRYGRNLTTGDVRGYDKDPGGG"
     gene            complement(815..982)
                     /locus_tag="VFF05_08915"
     CDS             complement(815..982)
                     /locus_tag="VFF05_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51970.1"
                     /translation="MRRALHLQGFFYFFNFSDVEKELARAVRYFWRTRKQQSVNQGST
                     SGRKDAGKPVA"
     gene            complement(1014..>1232)
                     /locus_tag="VFF05_08920"
     CDS             complement(1014..>1232)
                     /locus_tag="VFF05_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZW51971.1"
                     /translation="EKDGVKKWSTKIIADEMQMLGGREGGGAEGGSGGGYERRERGPA
                     RGPQSAPARSAPAAPAQSGNFDEDEIPF"
BASE COUNT          198 a          402 c          381 g          251 t
ORIGIN      
        1 tccgccgccg ggatccttgt cgtagccgcg cacgtcgccg gttgtgagat tacggccgta
       61 acgcatcttc tcgtcgagca tgccccactc acgcagcgat tccagcagct tttcgccatc
      121 ttccttggcg aattccttgt ccaacgcgcc ctgatcgacc gccttggcca gcagctcggc
      181 cagcatgcct tggtaatcgg ccttgtagct gcgatagcgc tgcggcttgc cgccgagcgc
      241 atgttcgccg tgcaggtagg cgttgtagtt gacctgcacg aagggctcca acgcgatgcc
      301 cagcacgcgg cagtaatgca acaggttttc gtgatggtag ggaatgcgcc acggcccggg
      361 gttgaggtac aggcccttgt cgaattcgca gtgttggcgc gcgccgccca gttcggtgta
      421 ggtatcgcca ccgcgcagcg tccagttgcg gccgccggga cgcgcgttgt attcgagcac
      481 ctgcacgcgg tagccggcgt tgcgcagctc gtaggccgca gtcatgccgg cgagcccggc
      541 gccgaggatc aataccgagg cgccgcgcgg tgcgccggaa agatccaccg gcccggcgta
      601 tttcgattcg cgcgtcgcgc cgagcgccgt catcgcgcgg tacatcgccg cgctgccggc
      661 aatcagcccg atactgcgca gcgcctcgcg ccggctcggt gaaaatcgag tgtgcgcatc
      721 ggacatggca acctccccgt tgcaggccga aaccgtacgg tgacttcttg ccgggcgagc
      781 ttagcgcgta tgggggtggg aagcacgtgg cagcctatgc aacgggtttg ccggcatcct
      841 tgcggcccga ggtcgatcct tggttcacgc tttgttgttt gcgtgtgcgc cagaaatacc
      901 gcaccgctct ggccaattct ttttcaacat cagagaaatt gaaaaaataa aaaaagccct
      961 gcaaatgcag ggctcttctc atccgtatat caactgttcg cgttatgtgt ttcctaaaag
     1021 ggaatctcat cctcatcgaa attgccgctc tgagcgggtg cggccggtgc gctgcgcgcc
     1081 ggcgcggatt gcgggccgcg cgcggggccg cgttcgcgac gctcgtagcc gccgccgctg
     1141 ccgccctccg cgccaccgcc ttcgcggcca ccgagcatct gcatctcgtc ggcgatgatc
     1201 ttggtcgacc acttcttgac gccgtccttc tc
//
LOCUS       DASRVO010000721         2756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_98409, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000721 DASRVO010000000
VERSION     DASRVO010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98409"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..446)
                     /locus_tag="VFF05_08925"
                     /pseudo
     CDS             complement(75..446)
                     /locus_tag="VFF05_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002814543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            672..1154
                     /locus_tag="VFF05_08930"
     CDS             672..1154
                     /locus_tag="VFF05_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZW51972.1"
                     /translation="MQAEKDKVVSIHYSVSEEPGVEIETSHGRPPMAFLFGHGNIIPG
                     LEQAIAGRAVGDTFDVVIEPEQGYGKRREDFVQRVPKKYFRDGEHLKPGMSTTLNTRD
                     GQRSVTVLKIGSSVVDVDLNHPLAGKTLRFAVEITDVRDASEEELAHGHAHGPEGHHH
                     "
     gene            complement(1374..1673)
                     /locus_tag="VFF05_08935"
     CDS             complement(1374..1673)
                     /locus_tag="VFF05_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51973.1"
                     /translation="MLLALRNPKSGWLATMICALEEALRDVDFSEHHRAMVKQLLEQG
                     AVTAAVSEAAEERLARFEASVLETQAGLAASVAAPLMATAALPARPKLTLVSNAA"
     gene            1895..>2756
                     /locus_tag="VFF05_08940"
     CDS             1895..>2756
                     /locus_tag="VFF05_08940"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW51974.1"
                     /translation="MNRRESDVLVLGGGAIGLACALYLLRAGRSVTVLDRGQVGAATS
                     HGNCGTITPSHAMPLAAPGVLAQALRWVLKKDAPLRISPRLDFATFEWLLQFARRCNA
                     TQCRRVTAIKVPLLMQARAELETLVRNAKLDCEFQMRGTMNVYRDARAFEHSRQMALL
                     PAEFGLPVEVLDGAACRAREPALNDSIVGGYFHAGDAQLRPERYCAELARAVAALGGE
                     IFESTTISRFRTVAGKIESVVTDRGEFCGRDIVFALGSWSPLLARQLDLRIPIQPGKG
                     YSITYTRPANP"
BASE COUNT          499 a          860 c          894 g          503 t
ORIGIN      
        1 tgctggtcat ccagcggccg aaggccccgt tgctcgacaa tttgcgcgtc gcgccggtca
       61 agtaagcgcg gtgactactt gaggatgcgc ttggcgccgc cgtagtgctc gcgccaatac
      121 gacgtgtcca ggtaatccag gcgcaccgtg ccgccggcat tcggcgcgtg cacgaaacgg
      181 cccttgccga catagatgcc gacatggctg atgtgtcgcc cttgcgtgtg gaaaaacacg
      241 atatcgccgg attcgagcgc gtcgcgcggc acgctgggcg tgtcgatgcg gctgatctcc
      301 tgcgccgtgc gtggcagttc gatgccggcc acgtcgcgga acacataatc cacaagtccg
      361 ctgcaatcga aaccgccgcg cggcgtgttg ccgccgtagc gataaggcgt gccgaccagt
      421 ccgatggcgc ggaacaggat gtcgttcgga ttcgcgccgt gcgccgcgcc cggataagtc
      481 acgggcggcg cggacttcgt gccgaggctg ccgcatccgg aaaagccggc tgccatgcca
      541 gcgaccaggc agcagaggaa gaatcgccga atgcgcatgg ccgcacactg ggtcgggcgg
      601 gcgcgtttgt caatgcggcg catgcgggcc gcgcgcaaaa cggttaggat gcggcgtttg
      661 cggggactgc catgcaagcc gaaaaagata aagtcgtaag cattcattac agcgtaagcg
      721 aagagccggg tgtcgagatc gagacttcgc acgggcggcc gccaatggcc ttcctgttcg
      781 gccacggcaa catcatcccg ggtctggaac aggccattgc cggacgcgcc gttggcgata
      841 ccttcgacgt ggtgatcgaa ccggagcaag gttacggcaa acgtcgcgag gacttcgtcc
      901 agcgcgtgcc gaagaagtat tttcgcgatg gcgagcactt gaaacccggc atgtcgacca
      961 cgttgaatac ccgcgacggc cagcgctcgg tcacggtgct caagatcggc tcgagcgtgg
     1021 tcgacgtcga cctgaaccat ccgctggccg gcaagacatt gcgcttcgcg gtggaaatca
     1081 ccgatgtgcg cgatgcgagc gaagaagaac tggcgcatgg gcatgcgcat gggccggaag
     1141 gccaccatca ctaaattaac cttgatggag cccgcgcaaa atcgtcgcga gcgaaggcag
     1201 aatgtgtgct gccggagcgc agaaaccgga gcatgctttt tcgtatgtga ggattttgag
     1261 caccgccagc acgcgagatg ccgaacgcag cagatttttc gcgggcgaca aaaagaggaa
     1321 gggcccggtt tgcaccaggc ccttccggac tccccttgcg attcggtgtt gccttacgcg
     1381 gcgttgctca ccagcgtcag tttcggacgc gccggcagcg cggcggtcgc catgagcgga
     1441 gcggcaacgg acgcggccag tccggcctgg gtttccagaa cgctcgcttc gaagcgggcg
     1501 aggcgttcct cggcggcttc ggacacggcg gccgtgacgg cgccctgttc cagcaactgc
     1561 ttgaccatgg cgcggtgatg ctcgctgaaa tccacgtccc tgagcgcttc ttccagcgca
     1621 cagatcatgg tggcaagcca gccggacttc ggattgcgca gcgcgagcag catttcagta
     1681 ttgggtgcgg gcatggccgt attccgcttt cattgccggc acctgccggc ggttgtgaat
     1741 atgcaggttc gatgccaacc gacccttatt tgtttgaaaa tggctctgta gagccatttc
     1801 catggtttcg tctggaccgg ttcgcgggtc cggcgcgcgc catccgccga tgtgcccggc
     1861 gcaatgcgtc aactttcgac ggaggttgcg tggcatgaat cgacgcgagt ccgatgtact
     1921 cgtcctcggc ggcggcgcga tcggccttgc ctgcgcgctg tacctgttgc gcgccggccg
     1981 cagcgtcacc gtgctcgatc gcggccaagt cggcgcggcg acctcgcatg gcaactgcgg
     2041 caccatcacg cccagccatg cgatgccact ggcggcgccc ggcgtgcttg cgcaggcgct
     2101 gcgctgggtg ctgaaaaaag atgcgccgct gcgcatttcc ccgcgtctgg atttcgccac
     2161 tttcgaatgg ctgctgcaat tcgcgcggcg ctgcaacgcc acgcaatgcc ggcgcgtcac
     2221 ggcgatcaag gtgccgttgt tgatgcaggc ccgcgccgaa cttgaaacac tggtccgcaa
     2281 cgcgaaactc gattgcgaat tccagatgcg cggcaccatg aacgtctatc gcgatgcgcg
     2341 cgctttcgaa cattcccggc agatggcgct gctgcccgcc gaattcggac tgccggtgga
     2401 agtactcgac ggcgctgcct gccgcgcgcg tgaaccggcg ctcaacgaca gcatcgtcgg
     2461 cggctacttc catgcgggcg atgcgcaact gcgacccgaa cgctattgcg ccgaactcgc
     2521 gcgtgcggtc gcggcgctgg gcggcgaaat tttcgaatcc accacgatca gcaggttccg
     2581 cacggtcgct gggaaaatcg aatccgtggt caccgaccgc ggcgaatttt gcggacgcga
     2641 catcgttttc gcgctcggtt cctggtcgcc gctgctggcc cggcaactcg acttgcgcat
     2701 tccgatccag ccgggcaagg gctattcgat cacttataca cggcctgcca atcctc
//
LOCUS       DASRVO010000722         1971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_47980, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000722 DASRVO010000000
VERSION     DASRVO010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47980"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1350
                     /locus_tag="VFF05_08945"
     CDS             <1..1350
                     /locus_tag="VFF05_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HZW51975.1"
                     /translation="RLEQNYRSTGNILNAANAIIANNPQRLGKKLWTAGEAGAAIALY
                     AAYNEQDEARFVVERIREHVQHGGNASDCAILYRSNAQSRNFEEQLIQHDIRYRVYGG
                     QRFFDRAEIKDALAYLRLVANRHDDAAFERAVNTPPRGIGERTLDTLRQRARATNASM
                     WDATQAEATGAALAGRAKNALKAFLALIDELAKTCASDGDAPMALSEQIDHAIQQSGL
                     RDYYEADSRGNAESRVENLDELVNVASRFERNAEDIEAGFSELTAFLAHAALEAGESQ
                     GEAWEDCVQLMTLHSAKGLEFPIVFLAGLEDGLFPSQKSLDEPGRLEEERRLAYVGIT
                     RARQSLVLSYAESRRLHGSETYNRPSRFLHELPRELLHEVRPRVQVSRPMYAGRFVDD
                     GGDAMPLKLGQHVRHANFGDGVVIDAEGAGAHARVQVNFENAGAKWLVVAYAGLTPL"
     gene            complement(1363..>1971)
                     /locus_tag="VFF05_08950"
     CDS             complement(1363..>1971)
                     /locus_tag="VFF05_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HZW51976.1"
                     /translation="KATLAPNPKVPWDTWVDDYATIRDAIEATYPEQFRDYNQRMWQP
                     GGFHRPNSARERIWKTKSGRAEFISPPAMVATGFTDAPGRFRLMTMRSNDQFNTTVYG
                     YDDRFRGIRGTRDVVLMNADDIAQAGLHEGQMVKLVSDAGDGIAREVAGLAVVAFDIP
                     RGCIGGYYPECNPLIPLSHHTVFSMLPAAKSVPVRIVADSAV"
BASE COUNT          367 a          635 c          644 g          325 t
ORIGIN      
        1 aggctggaac agaattaccg ttccaccggc aacattctca atgcggcgaa cgcgatcatc
       61 gcgaacaatc cgcagcgcct gggcaagaag ctgtggacgg cgggcgaagc cggcgcagcg
      121 atcgctttgt atgccgctta caacgaacag gacgaggcgc gcttcgtcgt cgagcgcatc
      181 cgcgaacatg tacagcacgg cggcaatgcc agcgattgcg cgatcctgta ccgctccaac
      241 gcgcagtcgc gcaacttcga ggaacaactg atccagcacg acatccgcta ccgcgtgtat
      301 ggcggccagc gcttcttcga ccgagccgaa atcaaggatg cgctggctta cctgcgcctc
      361 gtcgcgaatc gccacgacga cgcggcattc gagcgcgccg tgaacacgcc gccgcgcgga
      421 atcggcgaac gcaccctgga tacgctgcgt cagcgcgcac gcgccacgaa cgcgtcgatg
      481 tgggatgcga cgcaggccga agcgaccggc gcggcgctgg ccgggcgcgc gaagaacgcg
      541 ttgaaggcat ttcttgcgct gatcgacgag cttgccaaga cctgcgcatc cgatggcgat
      601 gcgccgatgg cgctgtccga gcagatcgac catgcgatcc agcaatcggg cttgcgcgat
      661 tactacgaag cagactcgcg cggcaacgcc gagtcgcgcg tggaaaacct cgacgaactg
      721 gtcaacgtcg ccagccgctt cgagcgcaac gccgaagaca tcgaggcggg attttccgaa
      781 ctcaccgctt tcctcgccca tgccgcgctg gaagccggcg aaagccaggg cgaggcctgg
      841 gaggactgcg tgcagctcat gaccctgcac tccgcaaagg gcctggagtt tcccatcgtg
      901 ttccttgcag gcctcgaaga cggattgttc ccgagccaga aatcgctgga cgaacccgga
      961 cgcctggaag aagaacggcg cctggcttac gtcggcatca cccgcgcgcg gcagtcgctg
     1021 gtgttgtcct acgcggaatc gcgccgcctg cacggatcgg aaacctacaa ccggccttcg
     1081 cgcttcctgc acgaactgcc gcgcgaactg ctgcacgaag tgcgcccgcg cgtgcaggta
     1141 tcgcggccga tgtatgcagg gcgtttcgtc gacgatggcg gcgacgccat gccgctcaag
     1201 ctcggccagc atgtgcgcca tgcaaacttc ggtgatggcg tcgtgatcga cgcggaaggc
     1261 gcgggcgcgc atgcacgcgt gcaggtgaat ttcgagaacg ccggagcgaa gtggctggtg
     1321 gtggcgtatg cgggattgac tccgctctga tccggcgcgt actcagaccg cgctgtcggc
     1381 cacgatccgc accggcaccg acttggccgc cggcagcatg ctgaacacgg tgtggtgcga
     1441 gagcgggatc agcggattgc attccgggta gtagccgccg atgcagccgc gcgggatgtc
     1501 gaacgcgacg acggcaaggc cggcaacttc acgcgcgatg ccgtcgccgg catccgagac
     1561 cagcttcacc atctggcctt cgtgcaggcc cgcctgtgcg atgtcgtccg cgttcatcag
     1621 cactacatca cgcgtgccgc ggataccgcg gaagcggtcg tcgtagccgt agaccgtggt
     1681 gttgaactgg tcgttggagc gcatcgtcat caggcggaag cgaccgggcg catcggtgaa
     1741 gccggtggcg accatggccg gcggcgaaat gaactcggcc ctgccgcttt tggttttcca
     1801 gatgcgctcg cgcgcgctgt tcggccgatg gaatccgccc ggctgccaca tgcgctggtt
     1861 gtaatcgcgg aactgctcgg gataagtcgc ctcgatcgcg tcgcgaatcg tcgcgtaatc
     1921 gtcgacccat gtatcccacg gcaccttcgg attcggcgcc agcgtggcct t
//
LOCUS       DASRVO010000723         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_98426, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000723 DASRVO010000000
VERSION     DASRVO010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98426"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFF05_08955"
     CDS             <1..547
                     /locus_tag="VFF05_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZW51977.1"
                     /translation="TAERGAQAQRNLHLTCFVIASREARDYLDRNCLAGLGWMSDRWW
                     KPALQSDRIAIENHSFDHNHAAIPLPGIDGMQRGTFHPVDSLVRADAEIAQAARSIDA
                     VIAPQRTSLFCYPYGHANDYLRTEYLALHQNEHGMRAAFGDGAQPVTIDSDRWNLPRY
                     ICGHHWQASEALRDILCAPPS"
     gene            638..1159
                     /locus_tag="VFF05_08960"
     CDS             638..1159
                     /locus_tag="VFF05_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51978.1"
                     /translation="MFDPREILPPALHDFLTITEIDHADFLVGPLFQRKFGEGPPDEP
                     RHLVCLYRDADGAQHLAGYSHMRPFGDVYLSGGSCTSGETLRRMRPHERAAVEASGGI
                     WLHLLKYAFRKYAGCCDAFFGHCGNPRALEVALAAGFAKTAHEPVIVRWHKPMHEVFR
                     RALLAKVVALGAF"
     gene            complement(1169..>1505)
                     /locus_tag="VFF05_08965"
     CDS             complement(1169..>1505)
                     /locus_tag="VFF05_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HZW51979.1"
                     /translation="DKPLSGGPFKGFLAALHRLQAAFPAGDDSPIRTALVTARSAPAH
                     KRVILTLRGWGVRLDEALFLGGRDKGPFLQTFGADIFFDDSHANVESARQHVATGHVP
                     HGVSNIKKR"
BASE COUNT          278 a          505 c          454 g          268 t
ORIGIN      
        1 caccgccgaa cgcggcgcgc aagcgcagcg gaatctgcac ctgacctgct tcgtgatcgc
       61 gtcacgcgaa gcgcgcgact atctggatcg gaattgcctg gccggactcg gctggatgag
      121 cgatcgctgg tggaaaccgg cgctgcaatc cgatcgcatc gccatcgaga atcacagctt
      181 cgaccacaac cacgccgcca tcccgctgcc cggcatcgac ggcatgcaac gcggcacatt
      241 ccacccggtc gatagccttg tgcgcgccga tgcggagatc gcgcaagccg cgcgcagcat
      301 cgatgccgtc attgcgccgc agcgcacgtc gctattttgt tatccgtacg gccacgccaa
      361 cgattacctg cgcaccgaat acctcgcgct tcaccaaaac gaacacggca tgcgcgcagc
      421 attcggcgac ggcgcacagc cggtgaccat cgacagcgac cgctggaacc tgccgcgcta
      481 catttgcggg catcattggc aagcatctga agctctgcgc gacatacttt gcgcgccccc
      541 aagctgaacg tccgaattga cgccggaacg gtcgctctct agcatcctat caacgcccgt
      601 gtggatatcc gaaatgtcat cttgcgagat cggtgaattg ttcgaccccc gcgagatttt
      661 gccacccgcg ttgcacgatt tcttgacgat cacggaaatc gatcacgccg attttctggt
      721 cggaccgctg ttccagcgca aattcggcga aggcccaccg gacgaaccgc ggcacctcgt
      781 ttgcctgtac cgcgacgcgg acggcgccca gcacctggcc ggctactcgc acatgcgccc
      841 atttggcgac gtctatcttt ctggcggctc ctgcaccagc ggcgaaacgc tgcgccgcat
      901 gcggccgcac gagcgcgcgg cggtggaagc cagcggcggc atctggttgc atctgctcaa
      961 atacgccttc cgcaaatacg ctggctgttg cgatgccttt ttcggacatt gcggcaatcc
     1021 gcgtgcactc gaagtcgctc tcgcggccgg ctttgcgaag acggcgcacg aaccagtcat
     1081 tgtgcgctgg cacaagccca tgcacgaggt cttcaggcgc gcactgctgg cgaaggtcgt
     1141 ggccctcggc gcgttctgag cgaccgcttc aacgcttctt gatgtttgaa accccgtgcg
     1201 gcacatgtcc tgtggcgacg tgctgccgcg cggattcgac gttggcgtgc gagtcgtcga
     1261 aaaagatgtc ggcgccgaag gtttgcagga acggaccctt gtcgcggccg ccgaggaaca
     1321 acgcttcgtc caggcgtacg ccccagccgc gcaacgtgag gatcacgcgc ttgtgcgcag
     1381 gcgccgaacg cgcggtgacg agcgcggtgc ggatcggcga gtcgtctccc gccggaaacg
     1441 ccgcctgcag gcgatgcagc gcggcgagaa atcccttgaa cggtccaccg gacagcggct
     1501 tgtcg
//
LOCUS       DASRVO010000724         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_384557, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000724 DASRVO010000000
VERSION     DASRVO010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384557"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..582)
                     /locus_tag="VFF05_08970"
     CDS             complement(94..582)
                     /locus_tag="VFF05_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HZW51980.1"
                     /translation="MARDKRGDEAISAYDKALGLLARREHSARELKSKLARKGLDAGE
                     SAEALKDLRAKDYQSDARFGEMLVRTRIAGGYGPRWILAELRQHGIAEDEAQAALGAA
                     GVDWSAQACDLLRRRYGNKRAATLAERAKRAAFLMRRGFDVHTVQSITRAAGPESDNE
                     SD"
     gene            complement(598..>1599)
                     /gene="recA"
                     /locus_tag="VFF05_08975"
     CDS             complement(598..>1599)
                     /gene="recA"
                     /locus_tag="VFF05_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZW51981.1"
                     /translation="VALAQIEKQFGKGSVMRLGDETRAPLEVIPTGSIALDVALGLGG
                     LPRGRVVEIYGPESSGKTTLTLQVIAQAQKLGGSCAFIDAEHALDPTYAEKLGVKVDD
                     LLLSQPDTGEQALEIADMLVRSGAVDVVVIDSVAALTPRAEIEGEMGDQLPGLQARLM
                     SQALRKLTGNIKRSNTMVIFINQLRMKIGMMMPGQNPETTTGGNALKFYASVRLDIRR
                     IGAVKKGEEVVGNQTKIKVVKNKLAPPFKQIVTEILYGEGISREGELIDLGVDNKLVE
                     KSGAWYSYAGERIGQGKDNARQYFKENPAIAQDLEDKLRAALGANKTVAPVVEMEEA"
BASE COUNT          250 a          527 c          504 g          318 t
ORIGIN      
        1 cgagaaaggt ctggcggatt tgcgcgctgg ttttcatgga tcgaatcgga atgggctcgg
       61 gacgcgacac gccggtcgca gtccatcgag cattcaatcc gactcgttgt ccgactccgg
      121 cccggcagcg cgggtgatgg attgtaccgt gtgaacgtcg aagccgcgac gcatcaggaa
      181 agccgcgcgt ttggcccgct ccgcgagcgt cgccgcacgc ttgttcccat accgacggcg
      241 caggaggtcg caagcctgcg cggaccagtc gactccggcg gcaccgagtg cggcctgtgc
      301 ttcatcctca gcaatgccat gctggcgcaa ctcggcgaga atccagcgtg gcccgtagcc
      361 gccggcgatg cgcgtgcgca ccagcatttc gccgaagcgc gcgtcggact ggtaatcctt
      421 cgcccgcaaa tccttgagcg cttcggcgct ttcgccagca tcaagaccct tgcgcgcaag
      481 cttggatttg agctcgcgcg ccgaatgctc gcgccgcgcg agcaggccga gggccttgtc
      541 gtaggccgaa atcgcctcgt cgccgcgttt atcccgtgcc acggccaaag cgccggttta
      601 cgcttcttcc atctccacca ccggcgccac ggtcttgttc gcgccgaggg cggcgcgcag
      661 cttgtcttca aggtcttgcg ctatggcggg attttccttg aagtactggc gcgcgttgtc
      721 cttgccctga ccgatgcgtt cgccggcata gctgtaccag gcaccggatt tctcgacgag
      781 cttgttgtcc acgcccagat cgatgagttc gccctcgcgg ctgatgccct cgccgtagag
      841 gatttcggtg actatctgct tgaacggcgg cgcgagcttg ttcttgacga ccttgatctt
      901 ggtctggttg ccgaccactt cctcgccctt cttcaccgcg ccgatgcgcc ggatgtccag
      961 acgcaccgag gcgtagaact tgagcgcgtt gccgccggtg gtggtttccg gattctgtcc
     1021 cggcatcatc atgccgatct tcatgcgcaa ctggttgatg aagatgacca tggtgttgga
     1081 gcgtttgatg ttgccggtaa gcttgcgcag cgcctgcgac atcaggcgag cctgcaagcc
     1141 gggcaactgg tcgcccatct cgccctcgat ctcggcacgc ggcgtgagcg cggcgaccga
     1201 gtcgatgacc accacgtcca ccgcgccgga acgcaccagc atgtcggcga tttccagcgc
     1261 ctgctcgccg gtgtcgggct gcgacagcag aagatcgtcc accttgacgc cgagtttctc
     1321 cgcataggtc ggatcgagcg cgtgttcggc atcgatgaag gcgcaactac cgccgagttt
     1381 ctgcgcttgc gcgatgactt gcagggtgag cgtggtcttg cccgaggatt ccggaccgta
     1441 gatttccacc acgcggccgc gcggcaggcc accgaggccc agcgccacgt cgagcgcgat
     1501 cgagccggtc gggatgacct ccagcggcgc gcgggtctcg tcacccaggc gcatcaccga
     1561 gcccttgccg aactgcttct cgatctgggc gagcgcgac
//
LOCUS       DASRVO010000725         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_203481, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000725 DASRVO010000000
VERSION     DASRVO010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203481"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VFF05_08980"
     CDS             complement(<1..818)
                     /locus_tag="VFF05_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HZW51982.1"
                     /translation="MSMPTHVRIVEVGPRDGLQNEKAIVPTAVKIELIDRLSETGLQT
                     VEATSFVSPKWVPQLADAADVYTSIRKKPGVRYPVLVPNLQGYERARSVGATEIAVFT
                     AASEAFNRKNINATIDESIDRFVPVMERARADGVGVRGYVSTVLGCPYQGEVPVTDVV
                     RVARRLHAAGCYEVSLGDTIGVGTPAKARAMLRAVAAEVPIEALAVHFHDTYGQALAN
                     ILACLEEGVRVVDSAVSGTGGCPYAKGATGNVASEDVVYMLHGLGLETGIDLDLL"
     gene            complement(815..>1015)
                     /locus_tag="VFF05_08985"
     CDS             complement(815..>1015)
                     /locus_tag="VFF05_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51983.1"
                     /translation="ETITQSPGSTRHEGDLHNFVAGDAAEMARVALLAGIFQANGYYT
                     LKPATTAEIFKPATNSPANAKP"
BASE COUNT          155 a          332 c          328 g          200 t
ORIGIN      
        1 agcaggtcga ggtcgatgcc ggtctccagt cccaggccat gcagcatgta gaccacgtcc
       61 tcgctggcca cgttgccggt ggcgcccttc gcgtaggggc agccgccggt gcccgagacg
      121 gcgctgtcga ccacgcgcac gccttcctcc aggcaggcca ggatgttcgc cagcgcctgg
      181 ccgtaggtgt cgtggaaatg cacggccagc gcctcgatcg gcacctcggc ggcgaccgcg
      241 cgcagcatcg cgcgcgcctt ggccggcgtg cccacgccga tggtgtcgcc cagcgacacc
      301 tcgtagcagc cggccgcgtg cagccgccgg gccacgcgta ccacgtcggt caccggcacc
      361 tcgccctggt agggacagcc gagcacggtg gagacgtaac cgcgcacgcc aacgccatcg
      421 gcccgggcgc gttccatcac cggcacgaaa cggtcgatgg attcgtcgat ggtcgcattg
      481 atgttcttgc gattgaacgc ctcgctggcc gcggtgaaca cggcgatttc cgttgcaccg
      541 acggatcgcg cacgctcgta gccttgcagg ttcggcacca gtaccggata gcgcacgccg
      601 ggttttttgc gtatggatgt ataaacgtcc gctgcatcgg ccagttgcgg cacccatttt
      661 ggactgacga agctggtcgc ttcgacggtc tgcaagcccg tttccgagag ccggtcgatc
      721 agttcgatct tgaccgctgt cggcacgatg gccttttcgt tttgcaaacc gtcgcgcgga
      781 ccgacttcga cgatgcggac gtgggtgggc atgctcatgg cttggcgttg gccggggaat
      841 tcgttgccgg cttgaatatt tcggctgtgg tcgccggttt caatgtgtag tagccgttcg
      901 cctggaaaat tccggcgagc agcgccacgc gagccatttc tgccgcgtcg ccggcgacga
      961 aattatgcag atccccttca tgtctcgtgc tcccggggct ttgcgtaatc gtctc
//
LOCUS       DASRVO010000726         1802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_73346, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000726 DASRVO010000000
VERSION     DASRVO010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73346"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VFF05_08990"
     CDS             complement(<1..1300)
                     /locus_tag="VFF05_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZW51984.1"
                     /translation="MDITNAEIAAVFAEIADWLELDAENPFRIRAYRNAARTVEAWPR
                     QLADMPRERREFAKLPGIGTDLGEKIGEILRSGTCSLLVRLRKSHPRGLTELLKVPGI
                     GPRRVARLHHELGITTLSGLVRAARARRISTLKGFGRRSEDTLLQAAVAHLGSERRWS
                     IAVASQHADAIMAYLRGSAAVLDIAVAGSFRRRRETVGDLDVLVSSRKPAQAAARFLA
                     YPQVARVLARGNTRCSAILKSGLQVDLRVVAPHSFGSALVYFTGSKAHNIALRRGAQQ
                     RGLKISEYGVFRGATRIAGATETDVYAAVGLSLIAPELREDRGEIQAARENRLPQLIE
                     RDDLRGDLHAHTNATDGHASIAEMAAAAKASGLSYLAITDHSLGLGIVHGLDASRLLR
                     QIEAIDRFNADVRGIAVLKGTEVEILEDGRLGLADDVLARL"
     gene            1489..>1802
                     /locus_tag="VFF05_08995"
     CDS             1489..>1802
                     /locus_tag="VFF05_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZW51985.1"
                     /translation="MDLKEAIYTRRAVRAFTAEAVDEKALRGLIDAAIQAPSAVNAQA
                     WAFCVVRDKAKLADISRRAKAHTLATTPAGLLSHHFETTLNDAQFNIFYDAPALIVIC
                     SR"
BASE COUNT          298 a          581 c          616 g          307 t
ORIGIN      
        1 ccagccgtgc cagcacgtcg tcggccaggc cgaggcggcc gtcttcgagg atctcgacct
       61 cggtgccctt gagcaccgcg atgccccgga catccgcgtt gaagcgatca atggcttcga
      121 tctgacgcag caggcggctg gcgtcgaggc catgcacgat gcccaggccg agcgaatgat
      181 ccgtgatcgc cagataggaa agcccggagg ctttcgctgc cgcggccatc tcggcgatgg
      241 aagcatgacc gtcggttgcg ttcgtgtgcg cgtgcaaatc gccgcgcaga tcgtcgcgtt
      301 cgatcagttg cggcagccgg ttttcgcgcg ccgcctggat ttcgccgcgg tcttcacgca
      361 gctccggtgc gatcaacgac aggccgacgg cggcgtagac gtcggtttcg gtcgcaccgg
      421 cgatgcgggt cgcgccgcgg aacacgccgt actcgctgat tttcaatccg cgctgctggg
      481 cgccgcgccg caaggcgatg ttgtgcgcct tggagccggt gaaatagacg agcgcggagc
      541 cgaagctgtg cggagccacc acgcgcagat cgacttgcag gccgcttttc aggatcgcgc
      601 tgcaacgcgt gtttccgcgt gcgagcacgc gtgcgacctg cgggtaggcg aggaagcgcg
      661 cagccgcttg cgccggtttg cgcgaggaca ccagcacgtc gaggtcgccg acggtttcgc
      721 gccggcgccg gaaactgccc gccaccgcga tgtcgagcac cgcagcgctg ccacgcagat
      781 acgccatgat ggcatcggca tgctggctgg cgaccgcgat cgaccagcga cgttcgctgc
      841 ccagatgggc gacggcggcc tgcagcaggg tgtcctcgct gcggcggccg aagcccttga
      901 gcgtgctgat gcgccgcgcg cgcgcggcgc gcacgaggcc cgacagtgtc gtgatgccga
      961 gttcgtggtg caggcgcgcg actcgcctgg gcccgatccc gggaaccttc aacagttcag
     1021 tgagaccgcg cgggtggctc ttgcgcaagc gcacgagcag gctgcaggtt ccgctgcgca
     1081 ggatctcgcc gatcttttcg ccgagatcgg ttccgatgcc cggcaatttc gcgaattcgc
     1141 gccgctcgcg cggcatgtcg gcgagttgcc ggggccaggc ttcgaccgtg cgcgcggcgt
     1201 tgcggtaggc gcgaatgcga aacggatttt ccgcgtcgag ctcgagccag tcggcgatct
     1261 cggcgaagac ggcggcgatt tcggcattcg ttatatccat ggggcaggcc ccggcgcaat
     1321 gtgatggcat cttatggcgc gtcatgcgtc cacgggtatt gatcgtggtc aagcggacgt
     1381 cgatgccgga acaagtgacg ctttcgtggt ttcgcgcaat ggcggcaatg acacgccgtg
     1441 ttgatctgga tcaacaggtg cgtccggatg cggcctaaat tccccaccat ggacctgaag
     1501 gaagccatct acacccgacg cgcagtacgg gccttcaccg ccgaagcggt cgacgaaaag
     1561 gctctgcgcg ggctaatcga cgccgcgatt caggcaccca gcgcggtgaa cgcgcaggcg
     1621 tgggcgttct gcgtggtgcg cgacaaggcc aagctcgccg acatctcgcg gcgggccaag
     1681 gcgcacaccc tcgcgaccac accggcagga ttgctatcgc atcacttcga gacgacgctg
     1741 aacgatgcgc agttcaacat cttctacgat gcgcccgcgc tgatcgtcat ctgcagccgc
     1801 ag
//
LOCUS       DASRVO010000727         1003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_384940, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000727 DASRVO010000000
VERSION     DASRVO010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384940"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VFF05_09000"
     CDS             <1..954
                     /locus_tag="VFF05_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZW51986.1"
                     /translation="ARCLYSAGVRRGDVVHNAYGYGLFTGGLGFHYGAERLGAVVVPV
                     SGGSTERQISLMMDFNARVLCATPSYALAIAEVAREQGVDLRNSGLRIGLFGAEPWSS
                     AMRREIEAQIGLRAVDTYGLSEILGPGVACECDCQNGLHGWEDHFLFEVIDPESGKPV
                     PEGQAGELVITTLTKQALPMLRYRTRDITRMTTARCDCGRTHARILRITGRNDDMLIV
                     RGVNVYPSQIEAVLLTRPNLAPHYQLVVERKGILDDVRIEVEALPGVSADGFRAIARD
                     VAHHIKSMIGISTDVMVQAPGTIPRSQGKAVRVRDLRPKEI"
BASE COUNT          171 a          332 c          340 g          160 t
ORIGIN      
        1 gcccggtgcc tttattcggc cggcgtgcgg cgcggcgacg tggtgcacaa cgcctacggc
       61 tatggtcttt tcactggcgg gctgggtttc cactacggcg ccgagcgact gggtgccgtg
      121 gtggtgccgg tttctggagg ctccaccgag cgccagatca gcctcatgat ggatttcaat
      181 gcgcgcgtgc tgtgcgcgac gccgtcctac gcactggcga tcgccgaggt cgcgcgcgag
      241 cagggcgtgg acctgcgcaa cagcgggctt cgcatcggct tgttcggcgc cgaaccctgg
      301 agttcggcga tgcgccgcga gatcgaagcc cagatcgggc tgagagccgt ggatacctac
      361 ggtctttcgg aaatcctggg ccccggcgtc gcctgcgaat gcgattgcca gaacggcctg
      421 cacggctggg aggaccattt cctgttcgag gtgatcgatc cggaaagcgg caagccggtg
      481 ccggaaggac aggctggcga actcgtgatc accacgctga ccaagcaggc gctgccgatg
      541 ctgcgctacc gcacccgcga catcacgcgc atgacgacgg cgcgctgcga ctgcggccgc
      601 acccacgcgc ggatcctgcg catcacgggc cgcaacgacg acatgctgat cgtccgcggc
      661 gtcaacgtgt acccgtcgca gatcgaggcg gtgctgctca cgcggcccaa cctcgcgccg
      721 cactaccagt tggtggtcga gcgcaagggc atcctggacg acgtgcgcat cgaggtcgag
      781 gcgctgcccg gcgtgagcgc cgacggtttc cgcgcgatcg cgcgcgacgt cgcgcaccac
      841 atcaagtcca tgatcggcat ctccaccgac gtcatggtgc aggcgccggg aaccatcccg
      901 cgctcccagg gcaaggcggt ccgcgtacgc gacctgcggc cgaaagagat ctgagcccga
      961 aatgagcaag cagatactcc aactcgaagt caatgggcgc tcg
//
LOCUS       DASRVO010000728         1597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_176142, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000728 DASRVO010000000
VERSION     DASRVO010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176142"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /gene="cmk"
                     /locus_tag="VFF05_09005"
     CDS             complement(<1..645)
                     /gene="cmk"
                     /locus_tag="VFF05_09005"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZW51987.1"
                     /translation="MQETEKKIVIAIDGPAGAGKSTIASRLARKFGYVNLESGAMYRA
                     LALKAIERDIGFEDEAALLDLAQRSKIELQPRLDGNRVLLDERDISVRIRERDVTEAA
                     SKVSVHPMVREWMVARQREMGEQGGVVMEGRDIGTKVFPHAEVKIFLEAEPAVRVDRR
                     VKQREAPPEQAPTIAAELLERDRRDSTRAASPLEPAGDAVILDSTHLSIDDVVAR"
     gene            complement(721..>1597)
                     /locus_tag="VFF05_09010"
     CDS             complement(721..>1597)
                     /locus_tag="VFF05_09010"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HZW51988.1"
                     /translation="RREKWRVAGNPARTLEDAREFLAANGFALMYPLRKQEQRVPVLA
                     PTFVGAYIGDDAKLPTWQQAFADPRALEATELMVRLLRERSAFELKFGETSFVTSATA
                     FPYFYGLVGDRNPRQQVAPGKASEYSPLARDVFEAIRTGGQLTKKQLRDVLGGAPSPA
                     ALDRALGELWARLRITRVDYTADEGAAWDALYRWAPEPVREGLNLSVVESLSALLSQY
                     LETTVAAEPSDAEDFFSYFVARSRVREAVNALLAARELSFTTIGNKTMLHITPRKQAF
                     DPARKRPIWRKKVTK"
BASE COUNT          264 a          567 c          480 g          286 t
ORIGIN      
        1 ccgcgccacc acgtcgtcga tgctcaggtg ggtggaatcg aggatgaccg cgtcgccggc
       61 aggttccagg ggcgaggccg cgcgcgtgga atcccgccgg tcgcgctcca agagttcggc
      121 ggcaatggtc ggcgcctgct cgggcggggc ctcacgctgc ttcacgcgcc ggtccacccg
      181 caccgccggt tccgcctcca gaaaaatctt aacttcggca tgaggaaaca ctttcgttcc
      241 gatgtcgcgc ccttccatca cgacgccgcc ctgctcgccc atctcgcgct ggcgggcgac
      301 catccactcg cggaccatgg gatggaccga gaccttcgac gcagcctcgg tcacgtcgcg
      361 ctcgcggatg cgcacgctga tgtcgcgctc gtccagcagc acgcggttgc cgtccaggcg
      421 cggctgcaat tcgattttcg agcgctgcgc cagatcgagc agggccgctt cgtcctcgaa
      481 accaatgtcg cgctcgatgg ccttcagcgc cagcgcccgg tacatggcgc cgctctccag
      541 gttgacgtag ccgaacttgc gcgccagccg cgacgcaatg gtgctcttgc cggcgcccgc
      601 cggcccgtca atcgcaatca cgatcttctt ttcagtttcc tgcattcggg acggagtgat
      661 ttctagctgc tcatcggtat tccgcgttcg ccgtatccca cttcgcacgt ggcacttcgc
      721 ctacttcgtc actttcttgc gccagattgg acgcttgcgc gctggatcga acgcctgctt
      781 acggggcgtg atgtgcagca tggtcttgtt gccgatcgtg gtaaagctca attcccgtgc
      841 cgccagcagg gcgttcaccg cctcgcgcac ccgcgagcgt gccacaaaat acgaaaagaa
      901 atcctcggca tcgctcggct cggccgccac cgtcgtttcc aaatactgcg acaaaagtgc
      961 ggacaaagat tcgacgaccg acaggttcaa cccctcgcgc accggctccg gcgcccagcg
     1021 atagagcgca tcccatgcgg cgccttcgtc ggcagtgtaa tccacgcgcg tgatgcgcag
     1081 gcgggcccac aactctccca acgcccggtc cagcgccgcc ggcgagggtg ccccgcccag
     1141 cacatcccgg agttgcttct tcgtcagttg tccgccggta cggatggcct cgaagacgtc
     1201 gcgcgccagc ggagaatatt ccgacgcctt gccgggcgcg acctgctgcc gcgggttgcg
     1261 gtcgcccacc aagccgtaaa aatatggaaa tgccgttgcc gaagtgacga agctggtctc
     1321 gccaaacttg agttcgaagg cgctccgctc ccgcagcagg cggaccatca actcggtcgc
     1381 ctcgagcgcg cgcgggtcgg cgaaagcctg ctgccaggtc ggcaacttcg cgtcatcgcc
     1441 gatgtatgcg cccacgaaag tcggcgccag cacgggaacg cgctgctcct gcttccgcag
     1501 cgggtacatc aacgcaaagc cgttggccgc caggaactca cgcgcgtcct cgagcgtgcg
     1561 cgccgggttg ccggccactc gccacttctc gcgccga
//
LOCUS       DASRVO010000729         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_98949, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000729 DASRVO010000000
VERSION     DASRVO010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98949"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFF05_09015"
     CDS             complement(<1..306)
                     /locus_tag="VFF05_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HZW51989.1"
                     /translation="MKASEIKKGNVVEHNGRVWQVRDIERSSPQGRGGNVTFRFTMYS
                     VPGAQKLDLSLRAEDDLKETELARRAATFSYKDGDAFVFMDAEDYTQYTLGADAIGDA
                     "
     gene            complement(303..1070)
                     /locus_tag="VFF05_09020"
     CDS             complement(303..1070)
                     /locus_tag="VFF05_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003486257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZW51990.1"
                     /translation="MQLDHIKAVVSGGVSGLGFAVARHLVAHGASVALFDINDEKGAT
                     AVAELGARASYQRVDVTSEDGVAAAVKQAAERMGGLNAAISCAGILGAGRVLGKEGPM
                     PLKTFQTTVLVNLIGSFNVAKSAADVMQHNAQGADGERGVIVNTASIAAYEGQIGQAA
                     YSASKGGVIGMTLPMAREFARIGVRVMTVAPGVFHTPMVDGMPQNVYDSLCAQVPYPS
                     RLGTAEEFADTVAFILQNRYMNGSVIRVDGAIRLAPK"
     gene            1239..2183
                     /locus_tag="VFF05_09025"
     CDS             1239..2183
                     /locus_tag="VFF05_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HZW51991.1"
                     /translation="MSLQRTSPPSASMRRLGRSHRYADAVLPERLRRWHAPRANRWER
                     LCVIAGRLVVELLEADGVVTQILIAGDDRWIAPGVRWRVARMDTDANFELEIHADDSS
                     LASAPQLVRAALLDDSDCVQPADEVEFAERLRELAPGRRWLVRARFDMGAAVCAAMAN
                     GAGTLCWHPLDAEQRSHTALVVRSAQAVGLADYLGRDHAVIEAALAGALRGDAERGRW
                     LRNLLARHLVIEEDLLFPAWLQTGGNPGWARGLRNEHAHLRRDLDHLDDAVARRRFLL
                     LLDGHDEKEEQIVYPDILARLGRDADRLAMEAMRMDLP"
     gene            complement(2232..>2476)
                     /locus_tag="VFF05_09030"
     CDS             complement(2232..>2476)
                     /locus_tag="VFF05_09030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZW51992.1"
                     /translation="CELRKMYFLPELRGRGAGAALIRHCMDTARSLGYAKCYLETLTG
                     MDAAQRLYEKHGFRRIPAACGATGHFSCDRFYLLNL"
BASE COUNT          399 a          801 c          817 g          459 t
ORIGIN      
        1 ggcgtcgccg atggcgtccg cgccgagcgt gtattgcgtg taatcctcgg catccatgaa
       61 cacgaacgcg tcgccgtcct tgtaggaaaa ggtcgccgcg cggcgcgcga gttcggtttc
      121 cttgagatca tcctccgcgc gcagcgagag gtccagcttc tgcgcgccgg gcacgctgta
      181 catcgtgaag cggaacgtga cgttgccgcc gcggccctgc ggcgagctgc gctcgatgtc
      241 gcgcacctgc cagacccggc cgttgtgctc gacgacgttg cctttcttga tttcggaggc
      301 tttcatttcg gtgccaaccg gattgcgccg tcgacgcgga tgacgctgcc gttcatgtag
      361 cggttttgca ggatgaacgc gaccgtatcg gcgaattcct cggccgtgcc caggcgcgac
      421 ggatacggga cttgcgcaca aagggaatcg tatacatttt gcggcatgcc atcgaccatc
      481 ggcgtgtgga acacgcccgg cgccaccgtc atcacgcgca cgccgatgcg cgcgaactcg
      541 cgcgccatgg gcagggtcat gccgatcacg ccgcctttcg aggcggagta agcggcctgg
      601 ccgatctggc cttcgtaggc ggcgatcgac gcggtgttga cgatcacacc gcgctcgccg
      661 tcggcgccct gggcgttgtg ttgcatcaca tcggcggcgg acttggcgac gttgaagctg
      721 cctatcaggt ttaccagcac cgtggtctgg aaggtcttga gcggcatcgg gccttccttg
      781 ccgagcacgc gccccgcgcc gaggatgccg gcacaactga tcgcggcgtt gagtccgccc
      841 atgcgttcgg cggcctgctt gaccgccgcg gcgacgccat cctcgctggt gacgtccacg
      901 cgctggtagc tcgctcgcgc acccaactca gcgaccgccg tcgcgccctt ttcgtcgttg
      961 atgtcgaaca gggcgacgct ggcgccgtgt gcgacaagat gcctggccac ggcaaagccg
     1021 agtccggaaa cgccgccgct gacgacggcc ttgatgtgat ccagttgcat gagtgatttt
     1081 ccgttgccgt ggttttcgcg tttacgggaa gtacgcaata cgtccaacct gatattttag
     1141 caggctgccg aagcggaaat cctgcccggc atccatgtgc ctcgcggcga catgatggaa
     1201 aatgcactct cgctgccagc cgcagggaaa cccaaaccgt gagcctgcaa cgcacgagtc
     1261 cgccatccgc gtccatgcgc cggctggggc gctcgcaccg ctacgccgac gctgttctgc
     1321 ccgaacgcct gcgccgttgg cacgcgccgc gcgcgaatcg gtgggagcgc ttgtgcgtga
     1381 tcgcgggcag gctcgtcgtc gaattgctgg aggccgatgg cgtcgtcacg caaatcctga
     1441 ttgccggcga cgaccgctgg atcgctcccg gcgtgcgctg gcgcgtcgcg cgaatggaca
     1501 cggacgcgaa tttcgaactg gagattcatg ccgacgattc cagtcttgcc agcgcgcccc
     1561 agctcgtgcg cgcggcattg ctcgacgact ccgactgcgt gcaacccgcg gacgaagtgg
     1621 aatttgcaga acgactgcgt gagcttgcgc cgggccggcg ctggttggtg cgcgcgcgtt
     1681 tcgatatggg tgcggcggtt tgcgcggcga tggcgaacgg cgccggcacg ctgtgctggc
     1741 atccgctcga tgccgaacag cgaagccaca cggcgctggt cgtgcgatcc gcgcaggccg
     1801 tcggcctcgc cgactacctg ggccgcgatc atgccgtgat cgaagcggcg cttgccggtg
     1861 cgttgcgcgg cgatgcggaa cgtggccgct ggttgcgcaa tttgctggcc cggcacctcg
     1921 tcatcgagga agatctgctg tttcccgcct ggctccagac cggcggcaat cccggctggg
     1981 cgcgtggttt gcgcaacgag catgcccatt tgcgtcgcga tctcgaccat ctcgacgatg
     2041 ccgtggcgcg gcgccggttt ctgctcctgc tcgacggcca cgacgaaaag gaagaacaga
     2101 ttgtgtatcc ggatatcctg gcacgcctcg gccgcgacgc cgatcggctg gcaatggaag
     2161 cgatgcgcat ggacttgccg tgagcggcag tcggcaccag gcacgccact gcgtgaacat
     2221 tactggcccg gctacaaatt cagcagataa aaccggtcgc aactgaaatg cccggtggcg
     2281 ccgcatgccg ccgggatgcg gcggaagccg tgtttttcgt acagccgctg cgcggcatcc
     2341 atgccggtga gcgtttccag atagcatttc gcatagccga gcgatcgcgc cgtgtccatg
     2401 caatggcgga tcagtgccgc gccggcgccg cgcccgcgca gttccggcag gaaatacatc
     2461 ttgcgcaatt cacagg
//
LOCUS       DASRVO010000730         1230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_21723, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000730 DASRVO010000000
VERSION     DASRVO010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21723"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VFF05_09035"
     CDS             <1..1026
                     /locus_tag="VFF05_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51993.1"
                     /translation="VLDDLTPLRQHAKLAANTDVLFDPAPATRVRGSQNKDTPPPADT
                     ALGENPPTGAVIDYRLVHNAQKVALQIRDAGGHVVRRFASDVPETVPDAERYFAKEWT
                     QPPAQLSAAAGMHRFIWDLRLPRPRAVHYDYGINAVWHDGTPILPQGMVVAPGDYTVA
                     LTVDGKTQTAPLKIVADPRVALDAAALNAALVFAGEVGNSLERDYVAYGQIKNVAEQL
                     GKLGDGAGGKPVQDAISKYKSAVGPLQSGAGADSENLDDIGGVLSAITADLEGSDRAP
                     TQPQRDVVAAADQRLERALKQWDGVRKSELVALNAALKKAGKEEIRVPAPDQVRLRDA
                     PESKDLP"
     gene            complement(1050..>1230)
                     /locus_tag="VFF05_09040"
     CDS             complement(1050..>1230)
                     /locus_tag="VFF05_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017917427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51994.1"
                     /translation="LGGIGAAVLGLVADHSGIVFVYQVCAFLPLLGIFAVFLPDVRPP
                     DRLQAQPADGVDPIG"
BASE COUNT          235 a          401 c          411 g          183 t
ORIGIN      
        1 gtgctggacg acctgacccc gctgcgccag catgcgaaac tggccgcgaa taccgacgta
       61 cttttcgatc ccgcgcctgc gacccgcgtg cgtggcagtc agaacaagga cacgccgcca
      121 ccggcggata ccgcgcttgg cgaaaatccg ccaaccggtg ccgtcatcga ttatagactt
      181 gtccacaatg cgcaaaaagt cgcattgcaa atccgcgatg cgggcggcca tgtcgtgcgc
      241 cgtttcgcga gcgatgtgcc ggaaactgtc ccggatgccg agcgctactt cgccaaggaa
      301 tggacgcagc cgccggcgca actttccgcc gccgccggca tgcaccgctt catctgggat
      361 ctgcgcctgc cgcgtccgcg cgccgtgcat tacgactatg gcatcaatgc ggtttggcac
      421 gatggcacgc cgatcttgcc gcagggcatg gtcgttgcac cgggcgatta caccgtggcg
      481 ctgaccgtcg atggcaagac ccagaccgcg ccgctcaaga tcgtggccga tccgcgcgtg
      541 gcgctggatg ccgccgcgct caacgccgcg cttgtctttg cgggcgaggt gggcaattcc
      601 ctggaacgcg actacgtcgc ttacggccag atcaagaacg tcgcggagca gcttggcaaa
      661 ctcggcgatg gtgcgggcgg caagccggtg caggacgcga tcagcaaata caaatccgcc
      721 gtcggcccgc tgcaatccgg tgcgggtgct gacagcgaga atctggacga catcggcggc
      781 gtgctttccg ccatcaccgc ggacctggaa ggcagcgacc gtgcgccgac gcagccgcag
      841 cgcgatgtcg tcgctgccgc cgatcagcga ctcgagcgcg cactcaagca atgggatggc
      901 gtgcggaaaa gcgagttggt cgcgttgaac gccgcgctga agaaggcggg caaggaggaa
      961 atccgcgtgc cggcgccaga tcaggtgcgc ctgcgcgatg cgccggagtc gaaggatttg
     1021 ccgtagtccg accgccccgg acaaacacct cagccgatgg ggtcgacgcc atcggctggc
     1081 tgcgcctgca accggtcggg tggccgcaca tcgggcagga atacggcgaa tatcccgagc
     1141 aggggcagga acgcacagac ctggtagacg aacacgatgc cggagtgatc ggcgaccagg
     1201 ccgagcacgg cggccccgat gccgcccagg
//
LOCUS       DASRVO010000731         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_125923, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000731 DASRVO010000000
VERSION     DASRVO010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125923"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /gene="metK"
                     /locus_tag="VFF05_09045"
     CDS             <1..949
                     /gene="metK"
                     /locus_tag="VFF05_09045"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZW51995.1"
                     /translation="QKVVRDVIKDVGYTRAKYGFDYETCAVLSSIHEQSRDIGQGVDT
                     GGAGDQGLMFGYACTQTTELMPLPIMLAHKLVRGMSELRRDGTLPYLRPDGKSQVTVE
                     YEGDKPVRIDTVVMSTQHSPSVTQDTIREDFIERVINNVIPLEMMDKKTKIYVNPTGR
                     FVIGGPHGDAGVTGRKIIVDTYGGYAPHGGGAFSGKDPTKVDRSACYMARYIAKNVVA
                     AGLAEKALVQLAYAIGVAEPVSVLVDTGNSGKVDDEKMTELVRAHFKLTPRGIMDTLD
                     LRRPIYRKTAAFGHFGRTEPEFTWERTDKASALRADAGP"
BASE COUNT          210 a          364 c          358 g          164 t
ORIGIN      
        1 ccagaaggtc gtccgcgacg tgatcaagga cgtcggctac acgcgcgcga agtacggatt
       61 cgattacgaa acctgcgcgg tgctctcatc cattcatgaa caatcgcgcg atatcggcca
      121 gggcgtggac accggcggcg ccggcgacca gggcctgatg ttcggctacg cctgcacgca
      181 gaccaccgag ttgatgccgc tgccgatcat gctggcgcac aagctggtgc gcggcatgtc
      241 ggaactgcgc cgcgacggca cgctgccata cctgcgtccc gacggcaagt cgcaggtgac
      301 ggtcgagtac gaaggcgaca agcccgtgcg catcgacacc gtcgtgatgt ccacgcagca
      361 cagccccagt gtgacgcagg acacgatccg cgaggacttc atcgagcgcg tgatcaacaa
      421 cgtgatcccg ctcgagatga tggacaagaa gacgaagatc tacgtcaatc cgacgggacg
      481 cttcgtcatc ggcggcccgc atggcgacgc cggcgtcacc gggcgcaaga tcatcgtcga
      541 cacctacggc ggctacgctc cgcacggcgg cggcgcattc tccggcaagg atccgaccaa
      601 ggtggaccgg tcggcctgct acatggcgcg ctacatcgcg aagaacgtgg tggccgccgg
      661 cctcgccgag aaggcgctcg tgcagctggc gtacgcgatc ggcgtggccg aaccggtgtc
      721 cgtgctggtt gataccggca actcgggcaa ggttgacgac gagaagatga cggaattggt
      781 gcgcgcgcac ttcaagctga ccccgcgcgg catcatggat accctggacc tgcgccggcc
      841 gatttatcgc aagacggcgg cgtttgggca tttcggacgc accgagcctg aattcacatg
      901 ggagcgcacc gacaaggcga gcgcgcttcg agccgacgcc ggcccgtagc cggcggaccg
      961 caccggtgag accctcgtgc tgtttcggcg attgatccag acaacggcgg ccctcgcggc
     1021 cgccgttgtc gcgtacacgg ccatcacgct gccgcccaaa cgcattgcca tcgaggcgcc
     1081 gctgccgccc accgcg
//
LOCUS       DASRVO010000732         1453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_204061, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000732 DASRVO010000000
VERSION     DASRVO010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204061"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFF05_09050"
     CDS             complement(<1..235)
                     /locus_tag="VFF05_09050"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HZW51996.1"
                     /translation="MSANSVFSWPVRVYWEDTDAGGVVYHAAYLRFLERARTEWLRAC
                     GVAQQRLREEHGILFVVADMQVKFLAPARLDDEL"
     gene            complement(232..1284)
                     /gene="ruvB"
                     /locus_tag="VFF05_09055"
     CDS             complement(232..1284)
                     /gene="ruvB"
                     /locus_tag="VFF05_09055"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZW51997.1"
                     /translation="MNDTRVIAPVATREDEIAEASIRPQKLAEYLGQQAVREQLSIYI
                     EAAKRRGEALDHVLIFGPPGLGKTTLSHVIANELGVNLRSTSGPVIERAGDLAALLTN
                     LQPRDVLFVDEIHRLSPVVEEILYPAMEDNQIDIMIGEGPAARSIKLDLPPFTLIGAT
                     TRAGLLTSPLRDRFGIVQRLEFYTPDELAAIVRRSAKILGIACADDGAAEIARRSRGT
                     PRIANRLLRRVRDFAQIKAGGTITREVAFAAMDMLKVDAEGFDALDRRLLETIIEHFD
                     GGPVGADSLAAALSEERGTIEDVLEPYLIQQGFLMRTARGRVATNKAYRHLGLKAPKR
                     EQRADLFVTPKDEDAP"
BASE COUNT          233 a          467 c          494 g          259 t
ORIGIN      
        1 gcagttcgtc gtcgagccgc gccggggcga gaaatttcac ctgcatgtcg gcgaccacga
       61 acaggatgcc gtgttcttcg cgcaggcgtt gctgcgccac gccgcaggca cgcagccact
      121 cggtgcgcgc gcgttcgagg aagcgcaggt aggcagcgtg gtagaccacg ccgccggcgt
      181 cggtgtcttc ccagtacacg cgcacgggcc aggaaaacac ggaattcgcg ctcacggtgc
      241 gtcttcgtcc ttgggcgtta cgaaaagatc tgcccgttgc tcccgcttcg gcgccttcag
      301 tcccagatgc cgataggcct tgttcgtggc cacgcgtccg cgcgcggtgc gcatcaaaaa
      361 tccctgctgg atcagatagg gttcaagcac gtcctcgatc gtgccgcgct cttccgacag
      421 cgccgcggcg agtgaatccg caccgaccgg accaccgtcg aagtgctcga tgatggtctc
      481 cagcaggcgc cggtcgagtg cgtcgaagcc ttcggcgtcg actttcagca tgtccatcgc
      541 ggcaaacgca acttcgcgcg tgatcgtgcc gccggccttg atctgggcga agtcgcgcac
      601 gcgccgcagc aggcgattgg cgatacgcgg cgtgccgcgc gagcgccgcg cgatttccgc
      661 cgcgccgtcg tccgcgcagg cgatgccgag gatcttcgcc gagcgccgca cgatcgcggc
      721 aagttcgtcc ggcgtgtaga actccaggcg ctgcacgatg ccgaagcggt cgcgcagcgg
      781 gctcgtgagc aggcccgcgc gcgtggtcgc gccgatcagg gtgaacggcg gcaggtcgag
      841 cttgatcgaa cgcgcggccg gaccctcgcc gatcatgatg tcgatctggt tgtcttccat
      901 cgccggatac aggatttcct cgaccaccgg cgacaggcga tggatttcgt cgacgaacag
      961 cacgtcgcgt ggttgcaaat tcgtgagcag cgcggccaga tcgccggcgc gctcgatgac
     1021 cggaccggaa gtgctgcgca agttcacgcc caactcattc gcgatcacat gcgagagcgt
     1081 ggtcttgccc agtcccggcg ggccgaagat cagcacgtgg tcgagcgctt cgccgcgccg
     1141 cttcgccgcc tcgatgtaga tcgaaagctg ctcgcgcacg gcctgctggc cgaggtattc
     1201 ggcgagtttt tgcgggcgga tcgaggcttc ggcgatttcg tcctcgcgcg tggcgaccgg
     1261 ggcgatgacg cgggtgtcgt tcatgcgccg attatcgcag gaaatggcgg tcaaccattg
     1321 cgtttcggat cggggcttgc cggcacaggg atgtgccgac gaatcgggcg cgtagcgctc
     1381 gggacggcaa acgtggcgcg gctttgccgc gaccgtttgc ttgcaaaccg cggcaggatg
     1441 cccggttttg caa
//
LOCUS       DASRVO010000733         1818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_48646, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000733 DASRVO010000000
VERSION     DASRVO010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48646"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFF05_09060"
     CDS             complement(<1..625)
                     /locus_tag="VFF05_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW51998.1"
                     /translation="MKLKLAFTLWLALFLACHCGAPAHAALTESDRRVYAEAFTAAQR
                     GDWARAYRLAATAHDPLPATALHWLQAKEANSGASFAAITGFIAAHPDWPAQRVLLER
                     AEDAIGTATDAQLNAWFKIHPPHLPVARLRQAQIWMDAGRRDDAVALIRKAWIEGDFT
                     AVEEKLMLQRYRGVLREEDDVRRLDRLIWDGQTREATRMLRHVSAGEA"
     gene            761..1636
                     /gene="dapA"
                     /locus_tag="VFF05_09065"
     CDS             761..1636
                     /gene="dapA"
                     /locus_tag="VFF05_09065"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZW51999.1"
                     /translation="MLKGSLVALITPFRGGAIDEKAFQDFVDWQIEQGTEGVVPCGTT
                     GESPTLSHAEHKRVVELCVEVARGRVPVVAGAGSNSTAEAIDLTRHAKEAGADAALVV
                     TPYYNKPTQEGLYQHYKAISDAVDLPIIIYNIPGRCVVDMSVATMARLAKLPNIVGVK
                     DATNDLARPLRTRGAIKDAFTLLSGEDATALAFLAQGGDGCISVTANVAPRACADMHA
                     AWRKGDAKAAMKINARLAPLHDALFVETSPGPVKYACSLIGRSTAEVRLPLVAASAQA
                     QEAVRTAMRSAGVLN"
     gene            1647..>1818
                     /locus_tag="VFF05_09070"
     CDS             1647..>1818
                     /locus_tag="VFF05_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HZW52000.1"
                     /translation="MTLRGDERYVALNRRARHDYFIEDTIEAGIELTGTEVKVLRRGL
                     GSIAEAYAAERGN"
BASE COUNT          313 a          596 c          609 g          300 t
ORIGIN      
        1 ccgcttcgcc ggcgctgacg tggcgcagca tgcgggttgc ctcgcgcgtc tgcccgtccc
       61 agatcaagcg gtcgaggcgg cgtacatcgt cttcctcgcg cagcacgccc cggtagcgct
      121 gcagcatcag cttttcttcg accgcggtga aatcaccctc gatccacgcc ttgcggatca
      181 gcgcaaccgc atcgtccctg cgtcccgcat ccatccagat ctgggcctgg cggagccgtg
      241 cgaccggcaa gtggggcgga tggattttga accaggcatt cagctgcgcg tcggtggcgg
      301 tcccgatcgc gtcttccgcc cgctcgagca gcacccgctg cgccggccaa tcgggatgcg
      361 ccgcgatgaa tccggtgatc gcggcaaagc tcgcgcccga attcgcttcc ttggcctgga
      421 gccaatgcag ggcggtggcg ggaaggggat catgcgccgt cgccgccagg cgataggcgc
      481 gtgcccaatc gccgcgttgt gccgccgtga aggcctcggc atagacgcgg cggtcgctct
      541 ccgtcagcgc agcatgtgcg ggcgcgccgc aatggcacgc aagaaacagc gccaaccaaa
      601 gagtaaaagc gagttttagc ttcattcgcc gccgaaccgc tgccacgata tcgcagctac
      661 aacttgcggc gaaacaatta atttactgtt gaccaatatt gcccgcctgg gcgccctggc
      721 ctatcgttga cgggccagag agcatcggtg gaggctagcc atgctgaagg gatcgctcgt
      781 tgcgctcatc acgccgttcc gcggcggagc gatcgacgag aaagcgtttc aggacttcgt
      841 cgattggcag atcgagcagg gtacggaggg tgtcgtgccc tgcggcacga ccggcgaatc
      901 gccgaccttg agccatgccg agcacaagcg cgtggtcgag ctctgcgtcg aggtggcgcg
      961 cggccgcgtg ccggtggtcg ccggggccgg ctccaactcg accgccgaag cgatcgacct
     1021 gacccggcat gccaaggagg ccggcgcgga tgccgcgctg gtcgtcacgc cctattacaa
     1081 caagccgacg caggaaggcc tctatcagca ctacaaggcg atcagcgacg cggtcgatct
     1141 gccgatcatc atctacaaca tcccggggcg ctgcgtcgtc gatatgtcgg ttgccaccat
     1201 ggcgcggctt gccaagctgc cgaacatcgt cggcgtgaag gacgcgacca acgatctcgc
     1261 ccggccgctg cgcacgcgcg gcgcgatcaa ggacgcgttc acgctccttt ccggcgagga
     1321 cgcgacggcg ctggctttcc tcgcgcaggg cggcgatggc tgcatttcgg tcacggccaa
     1381 tgtcgcgccc cgcgcctgcg ccgacatgca tgcggcatgg cggaagggcg atgcgaaggc
     1441 ggcgatgaag atcaatgcga ggctcgcgcc gctgcacgat gcgctcttcg tcgaaaccag
     1501 ccccggcccg gtgaaatatg cctgctcgct gattggccgg tcgacggcgg aggttcgcct
     1561 gccgctcgtc gccgcgagcg cgcaggcgca agaggccgtg cgtaccgcga tgcgttccgc
     1621 gggcgtgctc aactgagcga tatctgatga ctttgcgcgg cgacgagcgg tacgtcgcgc
     1681 tcaatcggcg ggcgcggcac gactatttca tcgaagacac gatcgaggcg ggaatcgagc
     1741 ttaccggaac cgaggtgaag gtgctgcgcc gcggcttggg cagtatcgcc gaagcctatg
     1801 ccgccgagcg cggcaacg
//
LOCUS       DASRVO010000734         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_229843, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000734 DASRVO010000000
VERSION     DASRVO010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229843"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VFF05_09075"
     CDS             <1..1299
                     /locus_tag="VFF05_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZW52001.1"
                     /translation="AIFDLMEKFAGYGFNKSHAAAYALVSYQTAWLKTHYPAQFMAAV
                     LSSDMDNTDKVVTFLTEARALGLNVQPPDVNASTYMFEATDPTTIRYGLGAVKGVGRN
                     AMENLVQARAQGGAFADLPDFCQRVDCQKVNKRVLEALILCGAMDALGANRASLMAQL
                     PECLRAAEQVARDAAAGQNDMFGAASATPAPRLQLATIPEWPPEQRLAGERDTLGHYL
                     SGHPTLLWRDTLAQLATCPIGEIGQRYQPPKPRADDDGNRFRRAPETPWVVAGQLVDL
                     RKRGTDQAFVQLEDFSGRIEVSLFREAFVESAALLTRDAILVVEGGLAWDDFAGALRI
                     RARRVFTLNEACERQAKLLRIRLNGIGEDFLDRLRDTLAGYRGGQTPLRLTYANRDGS
                     AEIELGAEWRVRATADLKRSLDVLPGVKSTEWVLGRSNGG"
     gene            1360..2316
                     /locus_tag="VFF05_09080"
     CDS             1360..2316
                     /locus_tag="VFF05_09080"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZW52002.1"
                     /translation="MNPNFLDFEQPIAELEGKIQELRHASHGQAFNIDDEVRKLRDKL
                     KRKTAEIFKDLTPWQIAQVARHPARPYTLDYVQAICEEFHELAGDRAYADDAAIVGGL
                     ARMDGRALMVIGQQKGRDTKTKIRRNFGMPRPEGYRKALRLMQMAERFGLPILTFIDT
                     PGAYPGVGAEERGQSEAIARNLLEMSQLRVPIVCTVIGEGGSGGALAIGVGDRTIMLQ
                     YSTYSVISPEGCASILWKSADRARDAAEALGLTAPRLLELGLIDKVVREPLGGAHRDP
                     HAMAIRLKAVLRNQLDELERSDRASLVGERYKRLRRYGAVKS"
BASE COUNT          442 a          845 c          772 g          371 t
ORIGIN      
        1 gcgattttcg acctgatgga gaaattcgcc ggttacggtt tcaacaagtc gcatgccgcc
       61 gcctatgcgc tggtttccta tcagaccgcg tggctgaaga cgcactatcc ggcgcagttc
      121 atggccgcgg tgctgtccag cgacatggac aacaccgaca aggtcgtgac attcctcacc
      181 gaggcgcgcg cgctggggtt gaacgtgcag ccgccggacg tgaacgcctc gacctatatg
      241 ttcgaagcga ccgatccgac gacgatccgc tacggactcg gtgcggtcaa gggcgtgggc
      301 cgcaacgcga tggaaaacct cgtgcaggcg cgcgcgcaag gcggcgcatt cgccgacctg
      361 cccgatttct gccagcgcgt ggactgccag aaggtcaaca agcgcgtgct cgaagcgctg
      421 atcctgtgcg gcgcaatgga cgcgctcggc gccaatcgcg ccagcctgat ggcccagctt
      481 cccgaatgcc tgcgcgccgc cgaacaggtc gcgcgcgatg ccgccgccgg gcaaaacgac
      541 atgttcggcg cggcgagtgc cacgccggcg ccgcgcctgc aactggcgac catccccgag
      601 tggccgcccg agcagcgcct tgccggcgaa cgcgacacgc ttggacacta tctttccggt
      661 caccccaccc tgctctggcg cgacacgctg gcgcaactgg cgacctgccc gatcggcgaa
      721 atcggccagc gctaccaacc gccgaagccg cgcgcggacg acgacggcaa ccgcttccgg
      781 cgcgcgccgg aaacgccatg ggtggtcgcc ggccaactcg tggacctgcg caagcgcggc
      841 accgaccagg ccttcgtgca gttggaggat ttcagcggcc gcatcgaagt gagcctgttc
      901 cgcgaagcct tcgtcgaaag cgcagcgttg ctcacgcgcg acgccatcct cgtcgtcgaa
      961 ggcggcctgg cctgggacga tttcgccggc gcgctgcgca tccgcgcacg gcgtgtattc
     1021 acgttgaatg aagcctgcga gcgccaggca aagctgctgc gtatccgtct caacggcatt
     1081 ggcgaggatt ttctcgatcg cctgcgcgat acgctcgccg gctatcgcgg cggacaaacg
     1141 ccgctgcgcc tgacctacgc caaccgcgac ggcagtgcgg aaatcgaact cggcgcggaa
     1201 tggcgcgtgc gcgccaccgc cgatctcaag cgcagcctcg acgtgctgcc cggcgtcaag
     1261 tcgaccgaat gggtgctcgg ccgctcgaat ggcggctgac gcggggcatt ccgccgcgcc
     1321 tgtggctata attcggagct tcgcgtccac gtttccggca tgaaccccaa cttcctcgat
     1381 ttcgaacaac ccatcgccga actggaaggc aagatccagg agctgcgcca tgccagccac
     1441 ggtcaggcat tcaatatcga cgacgaagtg cgcaagctgc gcgacaagct caagcgcaag
     1501 accgcggaaa tcttcaagga tctgacgccc tggcagatcg cgcaggtggc gcggcatccg
     1561 gcgcggccct acacgctcga ctacgtgcaa gcgatctgcg aggaattcca cgaactcgcc
     1621 ggcgatcgcg cctatgccga cgatgccgcc atcgtcggcg gcctcgcgcg catggacggg
     1681 cgcgcgctca tggtgatcgg ccagcagaag ggccgcgaca ccaagaccaa gatccggcgc
     1741 aatttcggca tgccgcgccc ggagggctac cgcaaggcgc tgcgcctgat gcagatggcc
     1801 gagcgcttcg gcctgcccat cctcaccttc atcgacacgc ccggtgcgta tcccggcgtc
     1861 ggcgccgagg aacgcggcca gtccgaggcg atcgcgcgca acctgctgga gatgtcgcag
     1921 ttgcgcgttc cgatcgtgtg caccgtgatc ggcgaaggcg gttccggcgg cgcactcgcg
     1981 atcggcgtcg gcgaccgtac catcatgctg caatactcga cctactcggt gatctcgccc
     2041 gaaggctgcg cctcgatcct gtggaagagt gccgaccggg cgcgcgacgc cgccgaagcg
     2101 cttggcctga ccgcaccgcg cctgctcgaa ctgggcctta tcgacaaagt ggtgcgcgaa
     2161 ccgctcggcg gcgcgcaccg cgatccgcac gcgatggcga tccgcctcaa ggccgtgctg
     2221 cgcaaccagc tcgacgagct cgaacgcagc gaccgtgcca gcctcgtcgg cgagcgctac
     2281 aaacgcctgc gccgctacgg cgcggtcaag tcgtagtctg ctcgacctcg atctcgatgc
     2341 cgccgaccat gctcgcaacg aggttgtaaa gcgcaccacc gatcgcgccc gcgataaagc
     2401 cgatcacacc gtacatgatc gggaaaatga
//
LOCUS       DASRVO010000735         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_73940, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000735 DASRVO010000000
VERSION     DASRVO010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73940"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFF05_09085"
     CDS             <1..477
                     /locus_tag="VFF05_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZW52003.1"
                     /translation="KACAETHGGTSRLDREAGPTFASVHVPTSCRHCEHPHCMKDCPP
                     DAIHRAPNGEVFIADNCIGCGNCERNCPYGVIHMSYKPPKKPGLIQWLLFGHGTGPGE
                     APHDAHDHAEGEIKKAVKCDMCKDVHGGPACVRSCPTGAAIRISPEEFPAYAQSRS"
     gene            486..1382
                     /locus_tag="VFF05_09090"
     CDS             486..1382
                     /locus_tag="VFF05_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52004.1"
                     /translation="MHDSILTYAKSRYLWIALVLTLASLVAYIAYDPGGPPNGGTWLG
                     YTLGTIGAALIVWLMLFGMRKRSYHNSPGTLRGWLSAHIYLGTSLILIVLLHSGFQFG
                     WNIHTLAFVLMLVVIFSGFFGVYVYLRYPNLMTRNRDSATRTAMLDEVAEIDQNALAL
                     ADGIDPKIHAVVLRSIQNTRLGGGVWTQLRARDGSDIALDSLHASLAERDKRSEMKTT
                     EMPTMFAMVDFLAGRATDKQGEAMRSLIDLISRKKGLATKVARDMQYQAMMEIWLYIH
                     VPLSFALLAALIAHIVSSFFYW"
     gene            1499..>2524
                     /locus_tag="VFF05_09095"
     CDS             1499..>2524
                     /locus_tag="VFF05_09095"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZW52005.1"
                     /translation="MRWLIRRVLKKGKGAVSYEEDVHYGDVLTIGRAADQAIFLPDLR
                     VALNHARVTLLSNGQHKIESLILAGIRVNDAITYATTAGPGAVVAIGNTRLTLLAAPQ
                     DYDAAVEVSTLDKSEQKAESAQRAKPTRLAQTGLSKRRPSWILFASILVFGLILPMIG
                     HFVPGFGEMLRRVPLLPSTQSWNPGTLDASHHFFAGDCTKCHQQAFLTVRDKACLACH
                     ANTAAHADPAKFNLPQLGNAQCRSCHQDHEGLRGLVRTDQRLCSDCHIDLKARTKGAS
                     TFADVGDFGTNHPEFKVNLPEWDANGKFAPQRTAWAEDLKENSGLKFNHEKHLKPGGL
                     NTPNGHRV"
BASE COUNT          472 a          846 c          772 g          434 t
ORIGIN      
        1 aaggcctgcg ccgagaccca tggcggcacc tcgcgcctgg accgcgaggc cggcccgacc
       61 ttcgccagcg tgcacgtgcc gacttcgtgc cggcactgcg agcatccgca ttgcatgaag
      121 gactgcccgc cggatgcgat ccaccgcgcg ccgaacggcg aagtgttcat cgccgacaac
      181 tgcatcggct gcggcaactg cgaacgcaac tgcccctacg gcgtgatcca catgtcgtac
      241 aagccgccga agaagccggg gctcatccag tggctgttgt tcggccacgg aacgggtccc
      301 ggcgaagcgc cgcacgacgc acacgaccat gccgaaggcg agatcaagaa ggcggtcaag
      361 tgcgacatgt gcaaggacgt gcatggcggc ccggcctgcg tgcgttcctg cccgaccggt
      421 gcggcaatcc gcatttcgcc ggaggaattc ccggcctacg cgcaatcccg cagctgagcg
      481 ccgcaatgca cgattccatc ctcacctacg ccaaatcgcg atacctgtgg atcgcgctcg
      541 tgctcacgct cgcctcgctg gtcgcgtaca tcgcctacga tccgggtggc ccgccgaacg
      601 gcggcacctg gctcggctac accctcggca cgattggcgc cgcgctcatt gtctggctga
      661 tgctgttcgg catgcgcaag cgcagctacc acaacagtcc cggcacgctg cgcggctggc
      721 tgtccgcgca tatctacctc ggcacgtcgc tcattctcat cgtgctgctg cacagcggtt
      781 tccagttcgg ctggaacatc cacaccctcg ccttcgtgct catgctcgtc gtgatcttct
      841 cgggcttctt cggcgtctac gtctacttgc gctatccgaa cctgatgaca cgcaaccgcg
      901 acagcgccac gcgcacggcc atgctcgacg aagtcgccga gatcgaccag aacgcgctgg
      961 cgctggccga tggcatcgac ccgaaaatcc acgccgtcgt gctgcgttcg atccagaaca
     1021 cgcgcctggg cggcggcgta tggacgcagt tgcgcgcccg cgacggctcc gacatcgcgc
     1081 tggattcgtt gcatgcgtcg ctggccgaac gcgacaagcg ctcggagatg aagaccacgg
     1141 aaatgccgac catgttcgcg atggtggatt tcctcgccgg ccgcgccacc gacaagcagg
     1201 gcgaggcgat gcgctcgctg atcgacctga tctcgcgcaa aaagggcctc gcaaccaagg
     1261 tcgcgcgcga catgcagtat caggcaatga tggagatctg gctgtacatc cacgtgccgt
     1321 tgtcgttcgc gctgctggcg gcgctgatcg cgcacatcgt gtcttcgttt ttttattggt
     1381 aactggacgt cgagcttgcg cgatgcgttg aagcgcatcg cgcgcgacgg caagctggtg
     1441 aggcaaggat gccgagcggc aacgcaccag ggacggtttg caagtggaga tttggtgtat
     1501 gcgctggctt atccgccgag tactgaagaa aggcaagggc gccgtctcct acgaggagga
     1561 cgtccactac ggtgacgtgc tcacgatcgg acgcgcggcg gaccaggcca tcttcctgcc
     1621 ggacttgcgc gtcgcgctca accatgcgcg cgtgaccctg ctttccaacg gccagcacaa
     1681 gatcgaatcg ctgatccttg ccggcatccg cgtcaacgat gcgatcactt acgcaacgac
     1741 cgccggaccc ggcgccgtgg tggcgatcgg caacacgcgc ctgacccttc tcgccgcgcc
     1801 gcaggattac gacgccgcag tggaagtatc gacgctagac aagagcgagc aaaaggccga
     1861 gagcgcgcag cgcgccaagc ccacgcgcct cgcacaaacg ggactgagca agcgccggcc
     1921 gtcgtggatc ctgttcgcgt cgatcctcgt tttcggtttg atcctgccca tgatcggcca
     1981 cttcgtgccg ggtttcggcg agatgctgcg gcgcgtgccg ctgctgccga gcacgcagag
     2041 ctggaatccc ggcacgctcg atgcgtccca tcatttcttc gccggcgact gcaccaagtg
     2101 tcatcagcag gcgttcctga ccgtgcgcga caaggcctgc ctggcctgcc acgcgaacac
     2161 cgccgcgcat gccgacccgg ccaagttcaa cctgccacaa ctgggcaatg cgcaatgccg
     2221 gagctgccat caggatcacg agggtttgcg cggcctggtt cgcaccgatc aacgtttgtg
     2281 ttcggattgc catatcgacc tcaaagcgcg caccaagggc gccagcacct ttgccgacgt
     2341 cggcgatttc ggcacgaatc atcccgaatt caaggtgaac cttcccgagt gggacgcgaa
     2401 cgggaaattc gcgccgcagc gcacggcctg ggccgaggac ttgaaggaaa actcgggcct
     2461 gaagttcaat catgaaaagc atctgaaacc cggcgggctg aacacgccga acggacaccg
     2521 tgtg
//
LOCUS       DASRVO010000736         2552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_333753, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000736 DASRVO010000000
VERSION     DASRVO010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333753"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="VFF05_09100"
     CDS             <1..1789
                     /locus_tag="VFF05_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZW52006.1"
                     /translation="KLAVSLGAQTANLPGESVAAELLAFARAHNVSKIIVGKPSRPRW
                     RDRLKASLVDEIIRGSGAIDVYVITGEEEASAPAARRLPSRSSKPFAYAQSALVVAGV
                     TLLCAALFGHIERANLVMIYLLGTVFVAARFGRGPSVMAAVLSVGLFDFLFVPPRYSF
                     AVSDAQYLITFAVMLVTALLISQLTARGRRQASIARQRERRTGELFTLSRELMHSRDL
                     AELGTILVRHVSAAIDGEAAVLLPDAEGHILDPTHFCTRGATPKHVMRYPVPGNDLGI
                     AQWGHDHRESAGCGTHTLASADAVYLPLNAPHRCIGVLGLRPRDPRQLEIPEQMHLLE
                     ALVNQAAIAMERVQLAQDARSADRQIEAERLRNLLLASISHDFRTPLAGIIGSASTLL
                     DSASTTLSEERRRSLLTALLAEAQRLHRLVGNLLDLTRLTSGPVQLKREWVALDELIG
                     AVLGRLGDVLAQREVTVDQPPDLPLVRCDEVMIEQVIFNLVENAHKHTPPGTPIRISA
                     RAWPALVEVRVADAGPGLPAGEESRVFEAFHRVRDESAQSGFGLGLAICKAVVDAHGG
                     EIRARNLPEGGAEFSFTLPREQEPPAAPP"
     gene            1786..2490
                     /locus_tag="VFF05_09105"
     CDS             1786..2490
                     /locus_tag="VFF05_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW52007.1"
                     /translation="MNAPQARVLVIEDDPQIRRFLAAALEAHDYALDMATTGMEGLRL
                     ATVRLPDIVIVDLGLPDISGLEVIERLRAWYTRAIVVLSARSQEADKVAALDRGADDY
                     LSKPFGVGELLARLRVAQRHVATRLGEEPQARIEVGALAIDLDARRVQRDGIDIHLTP
                     IEYKLLTVLARHRGKVMTHRQLLREVWGATHVESPHYLRIYLRALRHKIEPDPARPRY
                     LLTEVGVGYRLADSLP"
BASE COUNT          398 a          897 c          841 g          416 t
ORIGIN      
        1 caagctggcc gtgtcgctcg gtgcacagac cgccaacctc ccgggcgaat cggtcgccgc
       61 tgaattgctg gcgttcgcgc gcgcgcataa cgtcagcaag atcatcgtcg gcaagccgag
      121 ccggccgcgc tggcgcgacc ggctgaaggc ttccctcgtc gacgagatca tccgaggcag
      181 cggcgccatc gacgtgtacg tgatcacggg cgaagaggag gcatcggccc cggccgcgcg
      241 ccgcctgccg agtcgttcga gcaagccgtt tgcgtacgcg caaagcgcgc tcgtggtcgc
      301 cggcgtcacg ctgttgtgcg cggcattgtt cggccacatc gaacgcgcca acctggtcat
      361 gatctacctg ctcggcaccg tcttcgtcgc ggcgcgcttc ggacgcggcc cctcggtgat
      421 ggcggcggta ctgagcgtgg gcctgttcga tttcctgttc gtgccgccgc gctattcctt
      481 cgctgtctcc gacgcccagt acctgatcac gttcgcggtc atgctggtca cggccctgct
      541 gatcagccag ctgaccgcgc gcggccggcg ccaggcttcg atcgcgcgcc agcgtgaacg
      601 gcgcaccggc gagctgttca cgctgagccg cgaattgatg cacagccgcg accttgccga
      661 actcggtacg atcctggtgc ggcatgtgtc ggcggccatc gatggcgaag cggccgtgct
      721 gctgccggat gcggaaggcc acatcctcga cccgacgcat ttttgcacgc gtggcgccac
      781 gcccaagcac gtgatgcgct atcccgtccc cggcaacgac ctcggcatcg cgcaatgggg
      841 gcatgatcat cgcgaaagcg ccggctgcgg cacccacacg ctggccagcg cggacgcggt
      901 gtacctgccg ttgaacgcac cgcaccgctg catcggcgtg ctcggcctgc gcccacgcga
      961 tccgcgccaa ctcgaaatcc ccgagcagat gcacctactc gaggcgctgg tcaaccaggc
     1021 cgccatcgcg atggagcgcg tgcagcttgc gcaggacgcg cgcagcgccg accgccagat
     1081 cgaagccgag cgcctgcgca acctgctgct cgcttcgatc tcgcacgatt tccgcacgcc
     1141 gctcgccggc atcatcggct cggcatccac gttgttggac agtgcatcga cgacactgag
     1201 cgaggagcgt cgacgttcgc tgttgaccgc gttgctcgcc gaagcgcagc gcctgcaccg
     1261 tctcgtcggc aatctgctgg acctgacccg gctgacctcc ggcccggtcc agctcaagcg
     1321 cgagtgggtg gcgctggatg aactgatcgg cgcggtgttg ggtcggctgg gcgacgtgct
     1381 ggcccagcgc gaggtgacgg tcgaccagcc gccggacttg ccactcgtgc gctgcgacga
     1441 agtcatgatc gaacaggtga tcttcaacct cgtggaaaac gcgcacaagc acactccgcc
     1501 cggcacgccg atccggattt ccgcgcgcgc ctggccggcg ctggtcgagg tgcgcgtcgc
     1561 cgatgccggg cccggactgc ccgccggcga ggaaagccgg gtcttcgagg cgttccaccg
     1621 cgtgcgcgac gaatccgcgc aaagcggctt tggcctcggc ctggccattt gcaaggccgt
     1681 cgtcgacgcg cacggcgggg aaatccgcgc acgcaacctg cccgagggcg gcgccgaatt
     1741 cagttttacg ctgccgcgcg aacaagagcc gcccgcggct ccgccatgaa cgctccgcaa
     1801 gccagggtcc tcgtgatcga ggacgatccg cagatccggc gctttctcgc cgccgcgctg
     1861 gaagcgcacg actatgcgct ggacatggcc acgaccggca tggaaggcct gcgtctggcg
     1921 acggtgcgcc tgccggacat cgtcatcgtc gacctgggct tgcccgacat ttccgggctg
     1981 gaagtcatcg agcgtctgcg ggcgtggtac acgcgcgcca tcgtcgtgtt gtccgcgcgc
     2041 agccaggaag cggacaaggt cgccgcgctc gaccgcggtg ccgacgatta cctgagcaag
     2101 ccgttcggtg tcggcgagct gctcgcgcgc ctgcgcgtcg cgcagcgcca tgtggcgaca
     2161 cggctcggcg aagaaccgca ggcacgcatc gaagtcggcg ccctggccat cgatctcgac
     2221 gcccggcgcg tgcagcgcga cggcatcgac attcacctga ctccgatcga atacaaactg
     2281 ctgaccgtcc tcgcccggca tcgcggcaag gtgatgacgc accgccagct gctgcgcgaa
     2341 gtctggggcg cgacgcacgt cgagtcgccg cattacctgc gcatctacct gcgcgccttg
     2401 cgccacaaga tcgagcccga cccggcgcgt ccgcgctatc tgttgacgga agtcggcgtg
     2461 ggctatcgcc tggccgattc gctgccgtga aaacggctgc gtgacaatgc ccacgcgctt
     2521 cgtcgattcg tgtttgcgcg gccgcagtag ta
//
LOCUS       DASRVO010000737         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_308374, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000737 DASRVO010000000
VERSION     DASRVO010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308374"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..889)
                     /gene="rpe"
                     /locus_tag="VFF05_09110"
     CDS             complement(224..889)
                     /gene="rpe"
                     /locus_tag="VFF05_09110"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZW52008.1"
                     /translation="MKQPAVIAPSILSANFARLGEEVDNVLAAGADWVHFDVMDNHYV
                     PNLTIGPLVCEALRKHGVTAPIDVHLMVKPVDRLVPDFAKAGASLISFHPEASEHIDR
                     TLGLIRDSGCKTGLVFNPGTPLSYLDYVLDKLDLILIMSVNPGFGGQSFIPAALEKLR
                     RARELIDRSGRAIRLEIDGGVKIDNIADIAKAGADTFVAGSAIFGQKDYKATISAMRK
                     AIG"
     gene            complement(903..>1678)
                     /gene="djlA"
                     /locus_tag="VFF05_09115"
     CDS             complement(903..>1678)
                     /gene="djlA"
                     /locus_tag="VFF05_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HZW52009.1"
                     /translation="IAGKLVGALLGWVLLRNPIGLFIGFVLGHLWDQGTTRRAQSSPP
                     DAFIGPLFGLAGAIAKSDGRVSEPEIAATENLIARLRLSPAQRAEAIARFKEGKQDNF
                     AVDHAFAELRDWCRGRRDRAYILLDLLLEIVFAEGALTPEKLAIVRRLCASLGVHEQE
                     LAALAAMRGYGFGHAGGGYRQSQPPPGAPPGKDPYAVLGLSRDASDREIKTAYRRLIS
                     QHHPDKLGDVPEELKRRAEERAREINAAYEKIREERNIK"
BASE COUNT          287 a          530 c          555 g          306 t
ORIGIN      
        1 tatctgcccg ccgactatct gaatttgcgc gaagagctcg aagattggcg cgatgcgccc
       61 gcctgccgtt atgccgaagc gccgcagcat gagcacgagg cgcaacgaca aaacgaaaac
      121 gtcgatccga gctggctcac gctgtgattt ttcgacgcgc gccggggagc cggttcctcc
      181 cccaacctcc gagggttccc cggcgcgttt ttcgaacctg ggtttacccg atcgccttgc
      241 gcatcgccga gatcgtggcc ttgtaatcct tctgcccgaa gatcgccgaa ccggcaacga
      301 aagtgtcggc accggctttc gcgatgtccg cgatattgtc gatcttcacg ccgccgtcga
      361 tctccaggcg gatcgcacgg ccactgcggt cgatgagctc gcgcgcgcgg cgcaattttt
      421 ccagcgcggc cgggatgaag ctctgcccgc cgaagccggg attgaccgac atgatcagga
      481 tcagatcgag cttgtcgagt acgtaatcca gatacgaaag cggcgtgccg ggattgaaca
      541 cgagcccggt cttgcagccg ctgtcgcgga tcaggcccag cgtgcggtcg atgtgctcgc
      601 tggcctcggg atgaaaactg atcaggctcg cgccggcctt cgcgaaatcc ggtacgaggc
      661 gatccacggg tttgaccatc aggtgcacgt caatgggtgc cgttacgccg tgcttgcgca
      721 gcgcctcgca aaccagtggg ccgatggtca ggttcggcac gtaatggttg tccatcacgt
      781 cgaaatgaac ccagtccgcg ccggcggcga ggacgttgtc cacttcttca ccgagacgcg
      841 cgaagttcgc ggacaagatc gagggggcga tgacggcggg ttgcttcatg gcggactccg
      901 cattacttga tgttgcgttc ttcgcgtatc ttctcatatg ccgcgttgat ctcgcgcgcg
      961 cgctcttcgg cgcggcgctt gagttcctcg ggcacgtcgc caagtttgtc cggatggtgc
     1021 tggctgatca ggcgccggta ggcggtcttg atctcgcgat cgctggcatc acgcgacagg
     1081 ccgaggacgg cgtaagggtc cttgcccggc ggcgcaccgg gcggcggctg cgactgccgg
     1141 taaccgccgc cggcatggcc gaagccgtag ccgcgcatcg cggcgagtgc ggccagttcc
     1201 tgttcgtgca cgcccagcga agcgcacagg cggcgcacga tggcgagttt ttccggcgtc
     1261 agcgcgcctt cggcaaacac gatttccagc aacaggtcga gcaggatgta ggcgcggtcg
     1321 cggcggccgc ggcaccagtc gcgcagttcg gcgaaggcgt ggtcgacggc gaaattgtcc
     1381 tgcttgcctt ccttgaagcg cgcgatcgcc tcagcgcgct gcgcggggga aagccgcagg
     1441 cgcgcgatca aattttcggt ggcggcgatc tcgggttccg acacgcgtcc gtcggatttg
     1501 gcgatggcgc cggcgaggcc gaacagcggg ccgataaagg cgtcgggtgg gctcgactgc
     1561 gcgcgcctcg tcgtgccctg atcccacaga tggcccagca cgaagccaat gaacagcccg
     1621 atcggattgc gcaacagcac ccagccgagc agcgcaccga ccagcttgcc ggcgatat
//
LOCUS       DASRVO010000738         1030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_358640, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000738 DASRVO010000000
VERSION     DASRVO010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358640"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFF05_09120"
     CDS             complement(<1..232)
                     /locus_tag="VFF05_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011051113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HZW52010.1"
                     /translation="MDDNKRKALSAALSQIEKQFGKGAVMRMGDRTSDAIDAIPTGSL
                     MLDIALGIGGLPRGRVVEVYGPESSGKTTLTLQ"
     gene            complement(385..891)
                     /locus_tag="VFF05_09125"
     CDS             complement(385..891)
                     /locus_tag="VFF05_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZW52011.1"
                     /translation="MANTPATDAALHTLAEQIAVDFSRRGATLVTAESCSGGWIAKTL
                     TDIAGSSAWFECGVVAYSYEAKEALLGVRPETLEEHGAVSRETAIEMVSGALARYGAT
                     VAVAVTGIAGPSGGTADKPVGTVWISWKRRGGYAQAELFHFDGDREAVRRHTVAAALA
                     GTRKILTS"
BASE COUNT          156 a          339 c          333 g          202 t
ORIGIN      
        1 cctgcagcgt cagcgtcgtc ttgccggaag actccggacc gtacacctcg accacgcggc
       61 cgcgcggcag tccgccgatg ccgagcgcga tgtcgagcat gagggatccg gtcgggatcg
      121 cgtcgatcgc gtccgacgtg cgatcgccca tgcgcatgac cgcgcccttg ccgaattgtt
      181 tctcgatctg gctcaacgcc gcggaaagtg ccttgcgctt gttgtcgtcc attgcgatct
      241 ctgcctcgtg ttgatgacgt tggcggacag cgtgcgcgct atccgtctca tggattgcga
      301 tttcaaaccc tgctcaagcg cagagtttgt gcgatgggtg gattgtccca cagcgcggcg
      361 ggtgccgcat gcgcgaagcg cgcgtcagct tgtcagaatt ttccgagttc cggcgagtgc
      421 cgcggcgacg gtatggcggc gcacggcttc gcggtcgccg tcgaagtgga aaagttccgc
      481 ctgcgcgtag ccgccgcgcc gcttccaact gatccagacc gtgccaaccg gtttgtcggc
      541 ggtgccgcct gaaggtcctg caatgcccgt aactgcgacc gcaactgtcg cgccgtagcg
      601 tgcgagcgcg ccggagacca tctcgatcgc ggtttcgcga ctgacggcgc cgtgttcctc
      661 cagcgtttcc ggacgcacgc cgagcaacgc ctctttcgcc tcgtagctgt aggcgacgac
      721 gccgcactcg aaccacgccg acgatccggc gatgtcggtc agggtcttgg cgatccagcc
      781 gcccgagcac gactccgcgg tgaccagcgt cgcgccgcgc cgggaaaaat ccaccgcgat
      841 ctgctcggcg agcgtgtgca gggcggcatc ggtagctggg gtgttcgcca tcgtcggctc
      901 cggagaatgt gaagcgtgtt ctatccgatt ttcgcgcgcg gcgcaaagcc aattcggttt
      961 cgccgtcgcc gcattcctcg gacatggtct aagctctggg ctgctgcgcc gcattcccgg
     1021 acgatgacat
//
LOCUS       DASRVO010000739         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_255393, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000739 DASRVO010000000
VERSION     DASRVO010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255393"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFF05_09130"
     CDS             complement(<1..552)
                     /locus_tag="VFF05_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZW52012.1"
                     /translation="MLKIDTHAHILPARWPNLAEKYGDERFPVMVDADGRHRIYKDGK
                     FFREVWANAFDAEHRIADYAKFGVQVQAISTVPVLFSYWAKPNQALELHRFLNDHTAQ
                     ICRDHPRHYAGIGTVPLQSPTLAIQEMERCIEQLGLQGVQIGSHVDEWNLDAPELFPF
                     FEAAADLGAAILVHPWDMMGKDSM"
     gene            complement(631..1149)
                     /locus_tag="VFF05_09135"
     CDS             complement(631..1149)
                     /locus_tag="VFF05_09135"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HZW52013.1"
                     /translation="MLAPPIDFKKWIDEHRHLLRPPVGNKCIVDGDFIVMIVGGPNAR
                     TDYHWDEGPEFFHQIEGEMILRVQDDGRARDIPIRAGEVFYLPPRVPHSPQRMPDSIG
                     LVIERKRLAHEKDGLLWFCERCNHKLYEEFFTLGNIETDFPAVFERFYRSLDARTCKG
                     CGHVNPAPEKYR"
     gene            complement(1151..1576)
                     /locus_tag="VFF05_09140"
     CDS             complement(1151..1576)
                     /locus_tag="VFF05_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HZW52014.1"
                     /translation="MSDGIHIDAAPKPVGAYPHAQRVGNLLFLSGVGPRDPATNAIRG
                     NVYKADGTLATYDIAAQCRQVFANVRAVLEAAGARWEDLVDVTVFLTDMKRDFAAYNQ
                     VYAEYFTAAQPCRTTLGITALPTPIAIELKCIAALPEGR"
BASE COUNT          284 a          469 c          529 g          318 t
ORIGIN      
        1 catggaatcc ttacccatca tgtcccaagg atgcacgagg atcgccgcgc ccaggtcggc
       61 agcagcctcg aagaacggga acagttccgg cgcatcgagg ttccactcgt ccacgtgcga
      121 gccgatctgc acgccctgca ggccgagctg ttcgatgcag cgttccattt cctggatcgc
      181 cagcgtcggc gactgcagcg gcaccgtgcc gatgccggcg taatggcgcg gatgatcgcg
      241 gcagatctgc gcggtgtgat cgttcaggaa acggtgcagc tccagcgcct ggttcggctt
      301 ggcccagtac gaaaacagca ccggcacggt cgagatcgcc tgcacctgca cgccgaattt
      361 cgcgtagtcc gcgatgcggt gttcggcatc gaacgcgttt gcccaaacct cgcggaagaa
      421 tttgccgtcc ttgtagatgc ggtggcgtcc gtcggcatcg accatgaccg gaaagcgctc
      481 gtcgccatat ttctcggcga ggttcggcca gcgagccggc agtatgtggg cgtgggtgtc
      541 gatcttgagc atggagccga agtttaaccg ctggccacgc cgacggcgag cgacaacgtc
      601 accgccgcag tgtcgcgcaa cgcgcgcctt tcagcgatac ttttccggtg cggggttgac
      661 gtgaccgcaa cccttgcagg tgcgtgcgtc cagcgaacga tagaagcgct cgaatacggc
      721 cgggaaatcc gtttcgatgt tgccgagcgt gaagaattct tcgtaaagtt tgtggttgca
      781 gcgttcgcag aaccagagca ggccgtcctt ttcatgcgca agtcgtttgc gctcgatgac
      841 gagaccgatg gaatcgggca tgcgttgcgg cgaatgcggc acgcgcggcg gcagatagaa
      901 aacctcgccg gcacggatcg ggatgtcgcg cgcgcggcca tcgtcctgca cgcgcaggat
      961 catttcgcct tcgatctgat ggaagaattc cgggccttcg tcccagtggt agtcggtgcg
     1021 cgcgttcggg ccgccgacga tcatcacgat gaaatcgccg tcgacgatgc acttgttgcc
     1081 gaccggcggc ctgagcaggt ggcggtgctc gtcgatccat ttcttgaagt cgatgggcgg
     1141 ggcgagcatg tcagcgtcct tccggcagtg cggcaatgca cttcaattcg atcgcgatcg
     1201 gcgttggcag tgcggtaatg ccgagcgtgg tccggcacgg ctgggctgcc gtgaagtatt
     1261 cggcgtaaac ctggttatac gcagcgaagt cccgtttcat gtcggtgagg aacacggtga
     1321 cgtcgacgag gtcttcccag cgcgcgccgg ccgcttcgag tacggcgcgg acgttggcga
     1381 acacctgccg gcattgagcg gcgatgtcgt aggttgcgag cgtaccgtcc gctttgtata
     1441 catttccccg aatcgcgttc gtggccggat cgcgcggccc gacgccggac aggaacagaa
     1501 gattgccgac gcgctgcgcg tgcggataag cgcctacggg tttcggcgcg gcgtcgatgt
     1561 ggatgccatc gctcatggtt tcgcacttgc tgaaatagtg
//
LOCUS       DASRVO010000740         2685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_74048, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000740 DASRVO010000000
VERSION     DASRVO010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74048"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VFF05_09145"
     CDS             complement(<1..1350)
                     /locus_tag="VFF05_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW52015.1"
                     /translation="MNTSSERHWNRAQRYLAEGNFDAARTVLESFVQHNPQHTHAHLI
                     LGDIAWQDDRVRDSARHAHAAAASMPADPEIIVAVSSALLRAGEVVATRACLAHPALA
                     ATRSVPAILHIAGLRGMLNENAEALAQFDRAVAAGADGPDLRFQRGAQLIFNGRLPEA
                     ERELLECVRQNPRHGRAWQELSRLRKQTPADNHLDELARVTALVQPGSYEHASIEFAR
                     YKELEDLGRDDEAWAALAHGNALMYARHKHDPAHTERLFAALARACDSALLRPADVVH
                     AGSQPIFIIGMPRSGTTVLDRLLGNHTQIASVGELDDFGKQVRWVADHTATLDDAILE
                     RLPHMDFVELGRRYLEQTQWRAAGKPFYTDKLPRNWMLAGVIARALPQARILHMVRDP
                     MDVCFSNFRALFADAFAHIYDLEALASHYRVYQRTMAHWHQAMPGRILDVAYADLVQD
                     "
     gene            complement(1406..2428)
                     /locus_tag="VFF05_09150"
     CDS             complement(1406..2428)
                     /locus_tag="VFF05_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52016.1"
                     /translation="MLTPALALAQTVVPAPAGSAAVVIQPAGVSQDRADAGWRMPLWS
                     TSDGRILALVALGGGHGAPVLPQAPQIGSAADWQLIDVTHFVGGGLSLHLGNRVNAWA
                     RFGRGIALTPLNSAAATLGCDSELAFALNETCPYRGAPASTGALSIGTGLSTDRMDVG
                     VDYGYAWLRHETAPLSAIAQTPPWDLFATIGNEALPTLALPTLQWADVSDSAIRAQGH
                     WRWNDQNSLDVGAALSRLRFELPGNALLPTLNQAALSFGVRSGNFSGTLTGRVLGPAD
                     PLGSGQNWTSVDLGISWRAPWRGVFSIGAQNLWSSGTPPLLADPVHETDPSQARVPYV
                     QYHQDL"
BASE COUNT          473 a          826 c          943 g          443 t
ORIGIN      
        1 gtcctgcacc agatcggcgt aggccacatc gaggatgcgg cccggcatgg cctgatgcca
       61 atgcgccatc gtgcgctggt agacgcggta gtgagaggcg agggcttcaa ggtcgtaaat
      121 gtgcgcgaaa gcgtcggcga aaagagcgcg gaagttggaa aagcacacat ccataggatc
      181 gcgcaccatg tgcaggatgc gcgcctgcgg caacgcgcgg gcgatgacgc cggccagcat
      241 ccagttgcgc ggcagcttgt cggtgtaaaa cggtttgcca gcagcccgcc attgcgtctg
      301 ctcgagatag cgccggccta gttcgacaaa atccatgtgc ggcaggcgtt cgaggatcgc
      361 atcgtcgagc gtggcggtgt ggtcggcgac ccagcgtacc tgcttgccga aatcgtccag
      421 ttcgcccacc gaggcgattt gcgtgtggtt gccgagcaag cggtcgagca cggtggtgcc
      481 cgagcgcggc ataccgatga tgaagatcgg ctgcgaaccg gcatgcacga cgtcggccgg
      541 tctcagcaag gcgctgtcgc aggcgcgtgc cagggcggcg aaaagacgct cggtgtgcgc
      601 cgggtcgtgc ttgtggcgcg catacatcaa cgcgttgccg tgcgccagcg ccgcccaggc
      661 ttcgtcgtcg cggccgaggt cttcgagttc cttgtaacgc gcgaattcga tcgacgcatg
      721 ttcgtagctg cccggttgga ccagggccgt cacccgcgcc agttcgtcca gatggttgtc
      781 ggccggcgtc tgcttgcgca ggcgcgacag ttcctgccag gcgcgtccgt ggcgcgggtt
      841 ctgacgcacg cattcgagaa gttcgcgctc ggcctcgggc aggcggccgt tgaagatcag
      901 ttgcgcgccg cgctggaagc gcaggtccgg gccgtcggcg ccggcggcga cggcgcgatc
      961 gaattgcgcc agcgcctcgg cgttctcgtt gagcatgccg cgcaggccag cgatatgcaa
     1021 aatcgcggga accgatcggg tcgcggcaag tgccggatgc gccaggcacg cgcgcgtggc
     1081 cacgacttcg ccagcgcgca gcagcgccga ggataccgcg acgatgattt ccggatcggc
     1141 cggcatggac gccgccgcgg cgtgcgcatg ccgtgcggaa tcgcgcacgc gatcgtcctg
     1201 ccaggcgatg tcgccgagga tcagatgcgc gtgggtgtgt tgcggattgt gctgcacgaa
     1261 ggattcgagc acggtacgcg ctgcgtcgaa attgccttcg gccagataac gctgggcacg
     1321 attccagtgg cgttcggagg atgtgttcat gcggtgcgcg cgcgacggtc agcgcggcgt
     1381 gccgcaagcc gaagccgaag cgaggttaca aatcctgatg gtattgcacg tagggcacgc
     1441 gcgcctggct cggatcggtc tcgtgcacgg gatccgcgag caacggtggc gtgccgctgg
     1501 accacaggtt ctgcgcgccg atgctgaaga ctccgcgcca cggcgcacgc caggaaatgc
     1561 ccagatcgac gctggtccag ttctggccgc tgccgagcgg atcggccggt ccgaggacgc
     1621 ggccggtgag cgtgccgctg aaattgccgc tgcgcacgcc gaaactcagc gccgcctgat
     1681 tgagtgtcgg cagcagagcg ttgccgggaa gttcgaagcg caggcggctc aatgcggcgc
     1741 cgacatccag actgttctgg tcgttccagc gccaatgccc ctgcgcgcgg atggcgctgt
     1801 cgctgacatc cgcccactgc agtgtgggca gtgccagcgt cggcagggct tcattgccga
     1861 tggttgcgaa caaatcccaa ggcggcgttt gcgcgatggc cgaaagcggc gcggtttcgt
     1921 ggcgcaacca ggcgtagccg tagtccacgc ccacgtccat ccgatccgtg ctcagtccgg
     1981 tgccgatgct cagtgcaccg gtgctggccg gcgcaccgcg gtagggacac gtctcgttca
     2041 aggcgaaggc gagctcggaa tcgcagccca gcgtggctgc cgcggaattg agcggcgtca
     2101 gcgcgatgcc gcgcccgaac ctggcccagg cgttgacgcg gttgcctaga tgcagcgaca
     2161 gtccgccgcc gacgaaatgg gtgacatcga tgagttgcca gtcggcggcc gagccgatct
     2221 gcggcgcctg tgggagcacg ggcgcgccat gaccgccgcc gagtgcgacg agggcgagga
     2281 tgcggccatc ggacgtactc cacaacggca tgcgccagcc ggcatcggcg cggtcttgcg
     2341 aaacaccggc gggctgaatc acaaccgcgg cactgcctgc cggtgcgggc acgaccgtct
     2401 gcgccagcgc cagggcgggc gtaagcaagg cgctgaagag ggcggtggca aaacgcatat
     2461 gcatccggtc gagtcggtgg aactttgaac gcgtcccgcg tggcgaagtt ccaagctccg
     2521 cacgcaaagt cgcttattcg cagtatagcg caaatcacgg aaaactaaca attactttcg
     2581 acaggggccg agtcactgcg ctgctgcaaa aaatcgttaa actaaaccaa ttccagtctg
     2641 gggtcagcag gccttgtctt cacgcacgtc gagcatggtt gtgac
//
LOCUS       DASRVO010000741         1066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_308446, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000741 DASRVO010000000
VERSION     DASRVO010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308446"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1066
                     /locus_tag="VFF05_09155"
     CDS             <1..>1066
                     /locus_tag="VFF05_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZW52017.1"
                     /translation="LGVPGEDLPFVQYQLDDPDEYSGETIVVIGAGDAAIENAVALSK
                     QNNVVIVNRKDEFARAKKGNEQAILKAIADGVIECYYNSGPERVDALSVGRKKGRMIL
                     NTASGKAQILIDRIIARLGATAPRGFVEACGVTFPNKDPASVPAISSQYESNVKGLYI
                     VGALGGYPLIKQAMNQGYEVIEYILGNKVEPADEPLLKAKFAKMPGFRSVDDALARIQ
                     KNVPLLASITPLQLREFMLDSDIRSPAPGETIFTRNDYTNTFFSVVDGEAQVVVDEAT
                     NKRIALRRGEFFGEMSLISGRRRSATILAGANCVLIETPRRSMNRLIASVEAVKRHFD
                     EVFILRALQSRFAPEASAEQL"
BASE COUNT          216 a          349 c          328 g          173 t
ORIGIN      
        1 ctcggcgtgc ccggcgaaga cctgccgttc gtgcaatacc agctcgacga tccggacgaa
       61 tactccggcg aaaccatcgt cgtgatcggc gccggcgacg cggcgatcga gaacgcggtg
      121 gcgctgtcga aacagaacaa cgtcgtcatc gtcaaccgca aggacgagtt cgcacgcgcc
      181 aagaaaggca acgagcaggc catcctcaag gccatcgcgg acggcgtcat cgagtgctac
      241 tacaactccg gacccgaacg cgtcgacgca ctcagcgtcg gccgcaagaa ggggcgcatg
      301 atcctcaaca ctgccagcgg caaggcgcag atcctgatcg accgcatcat cgcccgcctt
      361 ggcgcgaccg cgccgcgcgg tttcgtcgaa gcctgcgggg tgactttccc gaacaaggat
      421 ccggcttcgg tgccggcaat cagttcgcag tacgaatcca acgtgaaggg cctgtacatc
      481 gtcggcgcgc tcggcggcta tccgctgatc aagcaggcga tgaaccaagg ctacgaggtg
      541 atcgaataca tccttggcaa caaggtcgaa ccggccgacg agccgctgct caaggccaag
      601 ttcgcgaaga tgccgggctt ccgctccgtc gacgatgcgc tcgcacgcat ccagaagaac
      661 gtgccgctgc tggccagcat cacgccgctg caactgcgcg agttcatgct cgattcggac
      721 atccgctcgc cggcgccggg cgagacgatc ttcacacgca acgattacac gaacacgttt
      781 ttcagcgtcg tcgacggcga ggcgcaggtg gtcgtggacg aggccacgaa caagcgcatc
      841 gccttgcgcc gcggcgaatt cttcggcgag atgagcctga tttccgggcg ccgtcgctcg
      901 gcgacgattc tggccggcgc caactgcgta ctgatcgaga cgccacggcg ttcgatgaac
      961 cggctgatcg cctcggtcga ggcggtcaag cgccatttcg atgaggtatt catcctgcgc
     1021 gcgctgcaat cgcgctttgc gccggaagct tccgccgaac aacttg
//
LOCUS       DASRVO010000742         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_176498, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000742 DASRVO010000000
VERSION     DASRVO010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176498"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFF05_09160"
     CDS             complement(<1..241)
                     /locus_tag="VFF05_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52018.1"
                     /translation="MVEPGIRVLHLGKFFPPHPGGIERFVSDLATAQAAQGMEVRVLA
                     HAEPGTSRNRHFRAGDVEVDLAACHGQFVYAPVSPA"
     gene            complement(234..644)
                     /locus_tag="VFF05_09165"
     CDS             complement(234..644)
                     /locus_tag="VFF05_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HZW52019.1"
                     /translation="MNAVQITAALIGVLLAGTILLLVRRTHLHGSHALWWLAVAGAIL
                     LLGVFPPVVDWVGRLTGIHYPPVIPIIIGIGMILVRLLKMDIDRSRSEQQLRRLTQKL
                     AILEQELAQSRRDKPRKLSGKGAKVIPLPKSSDG"
     gene            complement(641..>1018)
                     /locus_tag="VFF05_09170"
     CDS             complement(641..>1018)
                     /locus_tag="VFF05_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZW52020.1"
                     /translation="SGANVAIGTFPQRLSKAKRLAWRYFQLITGIGVQDFTSGLRVYD
                     RRAIRVLAAREASLLDYQDLGVLMLLRQRGLRLCEVPTPMSQRRNGGSRVFSSWSMVM
                     RYMLHTTVLCFAQIGTARLAARA"
BASE COUNT          187 a          336 c          340 g          155 t
ORIGIN      
        1 aagccgggct caccggcgcg tacacgaact ggccgtggca ggccgccaga tccacttcga
       61 catcccccgc gcggaaatgc cggttgcgcg aggttccggg ttcggcatgc gcaagcacgc
      121 gcacctccat gccctgcgcc gcctgcgcgg tggccaggtc agagacgaaa cgctcgatcc
      181 cgcccggatg cggcggaaag aatttgccga ggtggaggac ccggatgccg ggctcaacca
      241 tcgctggatt tcggcagcgg aatcaccttg gcgcccttgc cggaaagctt gcgcggcttg
      301 tcgcgtcgtg actgcgccag ttcctgttcc aggatggcga gtttctgggt cagccgccgc
      361 agttgttgtt cagaacgcga gcggtcgata tccattttca gcaggcgcac caggatcatg
      421 ccgatgccga tgatgatcgg aatcaccggc gggtagtgaa tgccggtcag gcggcccacc
      481 cagtcgacca ccggcggaaa cacgccgagc agcaggatcg caccggccac cgccagccac
      541 cacagcgcat ggctgccgtg cagatgcgtg cgccgcacca gcaacaggat cgtgccggcc
      601 agcaagacgc cgatcagtgc ggcagtgatc tgcacggcgt tcatgcgcgg gccgccagtc
      661 gtgccgtacc gatttgcgcg aagcacagca cggtggtgtg cagcatgtag cgcatcacca
      721 tcgaccatga cgagaacacg cgcgagccgc cgttgcgccg ctgtgacatc ggcgtcggca
      781 cctcgcacaa gcgcaggccg cgctggcgca gcagcatgag gactcccagg tcctggtaat
      841 cgagcaggct ggcctcgcgc gcggccagca cgcggatcgc gcggcggtcg tacacgcgca
      901 gtccggaggt gaaatcctgc acgccgatgc cggtgatcag ttgaaagtaa cgccacgcga
      961 ggcgcttggc cttcgacagg cgttgcggaa aggtgccgat cgcgacgttc gcgccgct
//
LOCUS       DASRVO010000743         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_176542, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000743 DASRVO010000000
VERSION     DASRVO010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176542"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..361
                     /gene="hfq"
                     /locus_tag="VFF05_09175"
     CDS             89..361
                     /gene="hfq"
                     /locus_tag="VFF05_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003471569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HZW52021.1"
                     /translation="MSEGQSLQDPFLNALRRERVPVSVYLVNGIKLQGTIESFDQFVV
                     LLRNTVSQMVYKHAISTIVPSRNVRVGTEHEEHAHAAAVGAEVVES"
     gene            375..>1603
                     /gene="hflX"
                     /locus_tag="VFF05_09180"
     CDS             375..>1603
                     /gene="hflX"
                     /locus_tag="VFF05_09180"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HZW52022.1"
                     /translation="MFERSRKGERALLVQPHRVGENDPDALAEFRELARSAGASVVGA
                     LSARIERPNPKFFIGSGKAEEVKALKEAENADLILVNHALSPVQERNLEQFTQCRVID
                     RTGLILDIFAQRARSHEGKLQIELAQLKHMATRLVRGWTHLERQRGGAIGLRGPGETQ
                     LELDRRLLAARVKQLTQRLQKVETQRSTARRARERNALPLVSLVGYTNAGKSTLFNAL
                     TDAGVYVADQLFATLDPTLRRIDGLNCGPFVLADTVGFIRDLPHDLVAAFKSTLAETR
                     DADLLLHVVDCADPERDRRIEEVERVLAEIGAQDVPQILVFNKLDKISALVPDREDHE
                     AAIHGRTSQQEPRVDYLDGGKTPRVWVSATHAIGLDLLRDTIARSLQGERVRHWLHLP
                     VDAGRLRARLFAQGLVA"
BASE COUNT          305 a          538 c          506 g          254 t
ORIGIN      
        1 gtagagtgga agctgcttga ttggcggcct ggacccattg gccgcagctc gcgccggctg
       61 gcgcgccata actagaaacg gagtccccat gtccgaagga cagtcgctgc aagacccctt
      121 cctcaatgcg ctgcgccgcg aacgcgtgcc ggtgtccgtg tacctggtca acggcatcaa
      181 gctgcagggc acgatcgagt cgttcgacca gttcgtcgtg ctgttgcgca ataccgtcag
      241 ccagatggtg tacaaacacg ccatttccac catcgtgcca agccgcaacg tgcgcgtcgg
      301 caccgagcac gaagagcacg cccacgccgc ggccgtcggg gccgaagtcg tcgagagctg
      361 atttgtcgca aaccttgttc gaacgctccc gcaagggcga acgcgcgctg ctggtgcagc
      421 cgcaccgagt gggcgagaac gatcccgacg cgttggccga attccgcgaa ctggcgcgct
      481 cggccggcgc cagcgtggtt ggcgcgctaa gcgcacgcat cgaacggcca aatccgaaat
      541 tcttcatcgg cagcggcaag gccgaagagg tcaaggcgct caaggaagcc gagaacgccg
      601 acctgatcct ggtcaatcac gcgctatccc cggtgcagga gcgcaatctc gaacaattca
      661 cgcaatgccg cgtgatcgac cgcaccggat tgatcctcga catcttcgcc caacgcgcgc
      721 gttcgcacga gggcaaactc cagatcgaac tcgcgcagct caagcacatg gccacgcgcc
      781 tggtgcgcgg ctggacacat ctcgaacgcc agcgcggcgg cgcgatcggc ctgcgcggac
      841 ccggcgaaac ccagctcgaa ctcgaccgcc gcctgctcgc cgcgcgggtc aagcagctca
      901 cccaacgcct gcaaaaagtc gaaacgcagc gcagcaccgc gcggcgcgcg cgcgagcgca
      961 acgcgctgcc gctggtgtcg ctggtcggct acacgaacgc gggcaaatcc acgttgttca
     1021 atgcgttgac cgacgccggc gtgtacgtgg ccgatcaatt gttcgccacg ctcgatccga
     1081 ccctgcgccg catcgacggg ctgaattgcg ggccgttcgt gctggccgac acggtgggat
     1141 tcatccgcga cctgccgcac gatctggtcg ccgcgttcaa gtcgacgctg gccgaaacgc
     1201 gcgacgcgga tttgctgctg catgtcgttg attgcgccga tcccgagcgc gaccggcgca
     1261 tcgaggaagt cgagcgcgtg cttgccgaaa tcggcgcgca ggacgtgccg cagatactcg
     1321 tgttcaacaa gctggacaaa atttccgcgc tcgtccctga tcgggaagat cacgaagcgg
     1381 ccatccatgg ccgcacttct caacaagagc ctcgtgtgga ttacctggat ggcggcaaga
     1441 ccccccgcgt gtgggtgtcg gccacgcacg cgatcggact ggatctgctg cgcgacacga
     1501 tcgcgcgcag cctgcaaggc gagcgcgtgc gccactggtt gcatctgccg gtcgacgccg
     1561 gacgcttgcg cgcacgtctg ttcgcgcaag gcctggtggc aaa
//
LOCUS       DASRVO010000744         1231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_126300, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000744 DASRVO010000000
VERSION     DASRVO010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126300"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..669)
                     /locus_tag="VFF05_09185"
     CDS             complement(64..669)
                     /locus_tag="VFF05_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZW52023.1"
                     /translation="MYDMKNALARPYLMKARAEAAAATKARILASARDLLVAHSFEEM
                     TIEAIASGAETTARTVLRLFGSKQELFAQALHSLGPLGFAPITPDNLEALVSGTYDFY
                     DKVGDTVIRWLADEPRLPAMHEHLNIGRQSLRGWVAEAFAPTLRRRAGAARKELHDAL
                     IVAFDVYTWKLLRRDFGLSRRAAEAAVARMVRGLVREEMNG"
     gene            829..>1231
                     /locus_tag="VFF05_09190"
     CDS             829..>1231
                     /locus_tag="VFF05_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HZW52024.1"
                     /translation="MKFGHFCLPTFFPDVDRDPGQLMRRWLDLLAESEALGFDSLWAN
                     EHHFDPYGGIIPSPPTMLAALSQRTRRARLGTSIVVLPLHNPIEIAEQLAMVDLMSGG
                     RVEFGIGRGFVEFDYDRLHIPRENSQERMREQ"
BASE COUNT          220 a          401 c          389 g          221 t
ORIGIN      
        1 acgcgggcga tgccgaggct cggcggcaga ttgccgcccc ccgaccagtt gagccaaagc
       61 atctcagcca ttcatctcct ccctgacgag tccgcgaacc atgcgagcga ccgccgcctc
      121 cgcggcgcgg cgcgacaggc cgaagtcgcg ccgcagcagc ttccaggtgt aaacgtcgaa
      181 ggcgacgatc aacgcgtcgt gcaactcctt gcgggcagcg ccggcgcggc gtcgcagcgt
      241 tggcgcgaac gcctcggcga cccagccgcg caggctctgg cggccgatat tgaggtgctc
      301 atgcatcgcc ggaagccgcg gctcgtcggc gagccagcga atcaccgtgt cgccgacctt
      361 gtcgtaaaaa tcataggtgc cgcttaccag cgcctcgaga ttgtccggcg tgatcggtgc
      421 gaagccgagc gggcccaggg aatggagcgc ctgcgcgaac aactcctgct tgctgccgaa
      481 gaggcgcagc acggtgcgtg ccgtggtctc ggcgcccgaa gcgatcgcct cgatcgtcat
      541 ctcctcgaaa gaatgcgcga cgaggagatc gcgcgcgctt gcgaggatgc gagccttggt
      601 cgcggctgcc gcctcggcac gcgctttcat caaataggga cgggcgagag cgtttttcat
      661 gtcatacatc gtggcacgaa aattattttc gtgtcaatat gcatgtcacg aaattgttga
      721 gcaaaagagc gaagtgggca tggccgctgg cttgcggcga ttcgggtcgt tcctctccgg
      781 ccccgaaacg cgctagaaag aagcctagga tctggaggag cgccacccat gaaattcggg
      841 catttctgcc tgccgacctt tttcccggat gtcgatcgcg accctggcca gttgatgcgc
      901 cggtggctcg atctcctggc cgaaagcgaa gcgctgggct tcgattcgct ctgggcgaac
      961 gagcatcatt tcgatcccta tggcggcatc atcccctcgc cgccgaccat gctcgcggcg
     1021 ctgtcgcaac gcacgcggcg cgcccggctc ggcacctcca tcgtcgtgct gccgctgcac
     1081 aatccgatcg agatcgccga acagcttgcc atggtcgatc tcatgtccgg cggccgcgtc
     1141 gagttcggca tcggccgcgg cttcgtcgag ttcgattacg atcggctgca catcccgcgt
     1201 gagaattcgc aagagcgcat gcgcgaacag c
//
LOCUS       DASRVO010000745         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_74202, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000745 DASRVO010000000
VERSION     DASRVO010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74202"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFF05_09195"
     CDS             <1..598
                     /locus_tag="VFF05_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 4"
                     /protein_id="HZW52025.1"
                     /translation="GIDGILAMQAIFVLVVMVVLCQRYSTSPHGAEVALWAAAVAAFL
                     PFNFPRARAFMGDVGSGSVGFLIAIAAIWQNSSPYTAALSGLVAASAFVTDASCTLLS
                     RMLCGRRWYRAHREHLYQWMTRTGMSHAQVVAWYAGWNLLIVVPVLYWMNLRPFGAPP
                     KPGSAAAVVIYAFAVVVWICGKRWCLYKVKSKQRHGLA"
     gene            636..>1033
                     /locus_tag="VFF05_09200"
     CDS             636..>1033
                     /locus_tag="VFF05_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZW52026.1"
                     /translation="MHDLAMVWLAWTAVNWLRYSFEANPPSAWQLAPDTAIVLLVQGT
                     VLWWTGLYKGLWRFASLPDLWNIVRAAILGAIAVAVALFLYDRLDLVPRSVLALYPAI
                     LAVLLGTPRLAYRYWKDSRFELLARVPARR"
BASE COUNT          140 a          309 c          348 g          236 t
ORIGIN      
        1 cggcatcgac ggcatcctcg ccatgcaggc gattttcgtg ctcgtcgtca tggtggtgct
       61 ttgccagcgt tacagcacca gcccgcacgg cgcggaggtc gccttgtggg cggcggcggt
      121 ggccgcattc ttgccgttca atttcccccg tgcacgcgcg ttcatgggcg acgtcggcag
      181 cggcagcgtg ggttttttga tcgcgatcgc cgcgatctgg cagaattcgt cgccatacac
      241 ggccgccttg agcggcctgg ttgccgcgtc ggcgttcgtc accgatgcga gctgcacctt
      301 gctgtcgcgg atgctgtgcg gacgccgctg gtaccgggcg catcgcgagc atttgtacca
      361 gtggatgacg cgtaccggca tgtcgcatgc gcaagtagtg gcatggtacg cgggatggaa
      421 cctgctgatc gtcgtgccgg tactgtactg gatgaacctg cggccgtttg gcgcgccgcc
      481 caagcccggc agcgccgcgg cagtggtcat ttacgcgttt gccgttgtcg tgtggatttg
      541 tggaaaacgc tggtgtttat acaaagtcaa atcgaagcag cggcatgggc ttgcgtaggt
      601 tgaccgccgc cattcacccg cgcctggctg tcgtattgca cgacctggcc atggtctggc
      661 ttgcctggac ggcggtgaac tggctgcgct acagcttcga ggccaatcca ccctcggcgt
      721 ggcagctggc gccggatacc gccatcgtcc tgctggtgca gggcacggtg ctgtggtgga
      781 cgggcttgta caagggtttg tggcgtttcg ccagcctgcc ggatctgtgg aacatcgtac
      841 gtgccgccat tctcggcgcg atcgcggtag ccgtggcgct gtttctttat gatcgtctcg
      901 atctggtgcc gcgcagcgtg ttggcacttt atccggcgat tctggccgtg ttgctcggca
      961 cgccgcgctt ggcttatcgc tactggaaag acagccgctt cgagttgctc gcgcgcgttc
     1021 cggcacgccg cat
//
LOCUS       DASRVO010000746         2657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_176690, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000746 DASRVO010000000
VERSION     DASRVO010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176690"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFF05_09205"
     CDS             complement(<1..979)
                     /locus_tag="VFF05_09205"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HZW52027.1"
                     /translation="MIVEILRVLALSTLASSAAIVVVLLLRKPLRQRFGAQVAYALWA
                     LVPLAALVALLPAPVASVASMPAIVAAAPAMMQTLPLAVAAAPAFDPMPWLALVWLFG
                     TVGCAVLFVRQQRRFVRSLGHLSSCDDDTVHAQTTAGCPALVGAWRPRIVLPADFERR
                     YDNAERKLILAHERAHRARGDAQANALAAALRCLYWFNPLFHFAASRLRFDQELACDA
                     IVISRFPEARRPYADAMLKTQLADLGLPAGCHWQSSHPLKERIAMLKQPLPGRARRRI
                     GAACVAAIVAVASFAAWAAQPAVPAAPAAVPASARAKSPPPDVGNAPQTV"
     gene            complement(969..1337)
                     /locus_tag="VFF05_09210"
     CDS             complement(969..1337)
                     /locus_tag="VFF05_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HZW52028.1"
                     /translation="MPISEAESQVMEVLWERYPLGADEVVAALADSTDWQEPTVKTLL
                     NRLLKKRAIAAQRDGRRYLYRPLLKREDYVHVASKSLLDRLFDGRVAPLVAHFSQRHK
                     LSKKDIAELKRLIEEIDHDR"
     gene            1437..1925
                     /locus_tag="VFF05_09215"
     CDS             1437..1925
                     /locus_tag="VFF05_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012444982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HZW52029.1"
                     /translation="MPYTPILATLGYVLSPDRRRVLMIHRNARADDHQLGKYNGLGGK
                     LEADEDVVAGMRREIREEAGIECDELQLRGTISWPGFGKHGEDWFGFVFVITHFSGTP
                     LQGNAEGSLEWVDIEKIPGLPLWEGDRNFLPLVFDADPRPFHGVMPYRDGRMLSWNYS
                     RI"
     gene            complement(1930..>2657)
                     /locus_tag="VFF05_09220"
     CDS             complement(1930..>2657)
                     /locus_tag="VFF05_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZW52030.1"
                     /translation="HGGFIPYDATFLVFSDYARNAVRMSALIPAHAIHVYTHDSIGLG
                     EDGPTHQPVEHLASLRLIPKNRVWRPCDAVESAVAWKVAIESREGPSCLVFSRQNLAH
                     QPRTPEQIAAIEFGGYVLREAKNGKPDAILIATGSEVDLAVQSADALEAEGITARVVS
                     MPCTNLFDRQPQAYRDAVLPPACRKRVAIEAGVTDFWRKYVGLDGHVVGVDTFGASAP
                     AEALFPYFGFTVDAVVAAVKSLR"
BASE COUNT          516 a          839 c          860 g          442 t
ORIGIN      
        1 gcacagtctg gggtgcgttg ccaacgtcgg ggggcggcga ttttgcccgc gcagaggcag
       61 gaacagcagc cggagccgca ggcacggccg gctgcgccgc ccacgcggca aaactcgcca
      121 cagcgacgat ggcggcgaca caagcagcgc cgatcctccg gcgcgcacgg cccggcaagg
      181 gttgtttcaa catggcaatt ctctccttca gtggatggct ggactgccaa tggcagccgg
      241 ccggcagtcc aaggtcggcc agctgcgttt tcaacatcgc gtcggcataa ggccggcgcg
      301 cttccggaaa tcgggaaatc acgattgcat cgcaggccag ctcctgatcg aagcgcaggc
      361 gcgatgcggc gaagtggaaa agtggattga accagtacag gcagcgtagc gctgccgcga
      421 gtgcgttcgc ctgcgcgtcg ccgcgcgcgc gatgggcgcg ctcgtgcgcg aggatcagtt
      481 tgcgctcggc gttgtcgtag cggcgctcga aatccgcggg cagcacgatg cgcggacgcc
      541 aggcacccac cagcgcgggg cagcctgccg tcgtttgcgc atgtacggtg tcgtcgtcgc
      601 acgacgacaa atgcccgagc gagcgcacga agcggcgctg ttgacgcacg aacaacaccg
      661 cacatccgac cgtgccaaac agccacacga gcgcaagcca tggcatcgga tcgaaagccg
      721 gcgcagccgc caccgcaagc ggcagcgttt gcatcatcgc cggagctgcg gcaacgatag
      781 cgggcatcga tgcgacggat gccaccggcg ccggcagcag cgcgaccaat gcggccaacg
      841 gcaccaacgc ccacagcgca taggcgacct gcgcgccgaa gcgctgtcgc aacggtttgc
      901 gcagcagcag aacaaccacg attgccgcgc tcgaagcgag cgtggaaagc gcgagcacgc
      961 gcaggatttc aacgatcatg gtcgatctcc tcgatcaggc gcttcaattc agcaatatcc
     1021 tttttgctca gcttgtgccg ctgtgaaaaa tgcgcgacca gcggtgcgac gcggccatcg
     1081 aacaagcggt cgagcaggct cttgcttgcc acatgcacat agtcttcacg cttgagcaat
     1141 ggccggtaca gatagcggcg gccatcgcgc tgcgcagcga tcgcgcgctt tttcagcagc
     1201 cggttgagca aggtcttcac cgtcggctcc tgccagtcgg tgctgtcggc gagcgccgcg
     1261 acgacttcgt ccgctccgag cgggtagcgt tcccacaaca cttccatcac ctggctttct
     1321 gcttcgctga tcggcatgtc gattccaatt acgtctgtaa ttgatgtaac gattacatgc
     1381 gtaaatgtaa ttgtcaagct tgcagcggat acaattccgg cgacacggag agccacatgc
     1441 cctacacacc catcctcgcc acgctcggct acgtgctgtc gccggatcgc cgccgcgtgc
     1501 tcatgatcca ccgcaacgct cgcgcggacg atcaccaact gggcaagtac aacggccttg
     1561 gcggcaagct agaagctgac gaggacgtcg ttgctggcat gcgccgcgag atccgcgagg
     1621 aagccgggat agaatgcgac gagctgcaat tgcgcggcac gatcagttgg cccggcttcg
     1681 gcaagcacgg tgaagactgg tttggcttcg ttttcgtcat cacgcacttc tcgggcacgc
     1741 cgctgcaagg caacgctgaa ggctcgctgg aatgggtgga tatcgaaaaa attcccggcc
     1801 tgcccctgtg ggaaggcgac aggaatttct tgccgctggt gttcgatgcc gatccgcgcc
     1861 cgttccacgg cgtgatgccg tatcgcgacg ggcgcatgct gagctggaat tactcgcgca
     1921 tctgaggcgt cagcgcagcg atttgaccgc cgccacgacc gcatccacgg tgaagccgaa
     1981 atacgggaac agcgcctcgg cgggtgcgga cgcgccgaag gtgtcgacgc cgacgacatg
     2041 gccgtcgagg ccgacgtact tgcgccagaa atccgtcacg ccggcttcga tggcgacgcg
     2101 cttgcggcaa gccggtggca acacggcatc gcgatacgct tgcggctgac ggtcgaaaag
     2161 gtttgtgcac ggcatggaaa cgacgcgcgc agtgatgccc tcggcttcca gcgcgtcggc
     2221 ggattgcacc gcaaggtcga cttcggaacc ggtggcgatc aggatcgcat ccggcttgcc
     2281 gttcttcgcc tcgcgcagca cgtagccgcc gaattcgatt gcggcgattt gctccggcgt
     2341 gcgcggctgg tgggcgagat tctggcgcga gaacaccagg cacgacgggc cttcgcgcga
     2401 ttcgatggcc accttccacg ccaccgcgct ttccaccgcg tcgcaggggc gccacacccg
     2461 gttcttcgga atcaggcgca gcgacgcgag gtgctcgacc ggctggtggg tcggaccgtc
     2521 ttcgccaaga ccgatggaat cgtgcgtgta gacgtggatc gcgtgggccg gaatcagcgc
     2581 ggacatgcgc accgcgttgc gcgcatagtc ggaaaaaacg aggaacgtgg cgtcataggg
     2641 aatgaagcct ccatgca
//
LOCUS       DASRVO010000747         1784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_151203, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000747 DASRVO010000000
VERSION     DASRVO010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151203"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFF05_09225"
     CDS             <1..768
                     /locus_tag="VFF05_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017116553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW52031.1"
                     /translation="QPVGGMGRIGEAFGRALGQTIRYNAKVTRIEQDDHGVSAYFTDA
                     AKPGATQQARADWCLCTIPLSVLGQIPMNVGSAMADAISAVPYAASVKIGLQFKRRFW
                     EEDERIYGGITHTDLPIFTIGYPSADFFSRGKGVLLGAYTWGASAFEFTAMSPAERVR
                     EAVRQGALIHPQYASEFENGIAVAWHRVPSAMGCFGEWSDALRERHYDNLCQIDGRIA
                     LAGEHASYLPAWQEGAITSALDAIGRLHRRAVAGSTT"
     gene            765..1220
                     /locus_tag="VFF05_09230"
     CDS             765..1220
                     /locus_tag="VFF05_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZW52032.1"
                     /translation="MKRIAYCGFFIGLLLPLPIHAQSSQTEALLTPQALAHADGEQIF
                     RHICQGCHMADAHGATGAGNYPALAGDANLASADFVAATLLFGRRNMPAFGPRPDLQG
                     WDAFLHVDLSDAQIASVVNYVRGHFGNRYSGQLTAAEVAALHPATPRKP"
     gene            1301..>1784
                     /locus_tag="VFF05_09235"
     CDS             1301..>1784
                     /locus_tag="VFF05_09235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZW52033.1"
                     /translation="MKTLLVTGGAGFIGGNFVLGLLAEGGVRVVNLDKLTYAGNLDTL
                     ASLRGNADHIFVQGDIGDRALVAKLLAEHKPTAIVNFAAESHVDRSIDGPAEFVNTNV
                     VGTLALLECALGYWRAAPLDAQDGFRFLHVSTDEVYGSLGPVGRFTEDSPYQPNSPYS
                     A"
BASE COUNT          295 a          586 c          572 g          331 t
ORIGIN      
        1 cagccggtcg gcggcatggg ccgcatcggc gaggcgttcg gccgcgcact cgggcagacc
       61 attcgctaca acgccaaggt gacgcgcatc gaacaggacg atcatggcgt gagcgcgtac
      121 ttcaccgacg cagccaagcc cggcgcgacc caacaggcac gcgccgactg gtgcctgtgc
      181 acgattccgc tctcggtgct cggccagata ccgatgaatg tcggcagcgc catggcggat
      241 gcgatttccg ccgtgccgta tgcggcgtcg gtgaagatcg gcttgcaatt caagcgccgc
      301 ttctgggaag aggacgaacg catctacggc ggcatcaccc ataccgacct gccgatcttc
      361 accatcggct atccgtcggc ggatttcttc agccgcggca agggcgtgct gctcggcgcc
      421 tacacctggg gcgccagtgc attcgagttc actgccatga gtccggccga gcgcgtgcgc
      481 gaggcagtgc gccaaggcgc gctgatccat ccgcaatatg ccagtgagtt cgaaaatggc
      541 atcgccgtgg cctggcatcg cgtgccatcc gcgatgggtt gtttcggcga gtggtcggat
      601 gccttgcgcg agcgtcacta cgacaacctg tgccagatcg acggtcgcat cgcgctggcc
      661 ggcgaacatg cctcgtacct gccggcttgg caggaaggcg cgatcacctc ggcgctggat
      721 gcgatcgggc gcctgcatcg gcgcgcggtg gcggggagca cgacatgaag cgcatcgcct
      781 attgcggatt cttcatcggc ctgttgttgc cgttacccat tcacgcgcaa tcgtcgcaga
      841 ccgaagcgct gctgacgccg caggcgcttg cccacgcgga tggcgagcaa atcttccggc
      901 acatctgcca gggttgtcac atggcggacg cgcacggcgc caccggcgct ggtaactatc
      961 ccgcgctggc gggcgatgcg aacctggcct cggccgattt tgtggctgca acgctgctgt
     1021 tcggacgccg caacatgccc gcgttcggtc cgcgacccga tttgcaaggc tgggatgcgt
     1081 tcttgcatgt ggacctcagc gacgcgcaga tcgcctccgt ggtcaactac gtccgcggtc
     1141 atttcggcaa tcgttattca ggtcagctca ctgccgccga ggtagccgcg ctgcacccgg
     1201 caaccccgcg caagccctga acgcggcgac cgccggcatc ggttaaaatc cgggttttgc
     1261 agaagcgcgg cttcgtgtcg tgcacaaggc tgttttgcgt atgaagactc tcctcgtcac
     1321 cggcggcgcc ggtttcatcg gcggcaactt cgtgcttggc ctgctggccg agggcggcgt
     1381 gcgcgtggtg aatctggaca agctcaccta cgccggcaac ctcgacacgc tggcgagcct
     1441 gcgcggcaac gccgatcaca tcttcgtgca aggcgacatc ggcgatcgcg cactcgtggc
     1501 gaagctgctg gccgagcaca aaccgacggc tatcgtgaat ttcgccgccg agtcgcacgt
     1561 ggaccgctcc atcgacggtc cggcggaatt cgttaacacc aatgtcgtcg gcacgctggc
     1621 cctgctcgaa tgcgcgctcg gttattggcg cgcggcgccg ctggatgcgc aggacggttt
     1681 ccgtttcctg cacgtctcca cggatgaagt ctatggctcg ctcgggcctg tcggtcgctt
     1741 caccgaggat tcgccgtacc agccgaactc gccatactcg gcat
//
LOCUS       DASRVO010000748         1299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_282765, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000748 DASRVO010000000
VERSION     DASRVO010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282765"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VFF05_09240"
     CDS             complement(<1..1266)
                     /locus_tag="VFF05_09240"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52034.1"
                     /translation="MRACLARQAPANEEELKRMLRQLRQQVMLRVMLRDLSGRAPLTE
                     VVGSMSDLAELGLDYALGYLSLWLRAQHGTPSANGRAQELIVVGMGKLGGRELNVSSD
                     IDLIFVYPEEGETVGSAGQSGAGAALRLLSNHEYFTRLGRKLINVLADITEDGQVFRV
                     DMRLRPNGDSGPLACSLDALENYFIAEGREWERYAWIKARVVATSGDPSALATSSNAS
                     VAASGDAGIPAARSKGAGDENTSAQQLGAVARPFVFRKYLDFGAFAAMRSLHAQIRAE
                     VARRDRAGHIKLGPGGIREIEFTAQVFQLIRGGSDARLRIRPTRSVLQRLAENAQMSL
                     QDVAALDAAYVFLRNLEHRLQYLDDRQTQELPETPEDREIIACGMGYADYAAFITELD
                     RHRKLVSRQFELIFGAQPLLQPGPPMTEQA"
BASE COUNT          210 a          418 c          425 g          246 t
ORIGIN      
        1 tgcttgctcc gtcatgggcg ggccgggctg cagcagtggt tgcgcgccaa aaatcagttc
       61 gaactgccgg ctgaccagct tgcggtggcg atcgagttcc gtgatgaagg cggcgtaatc
      121 agcgtaaccc ataccgcacg cgataatttc gcggtcttcc ggagtttccg gcaattcctg
      181 ggtttgccgg tcgtccagat actgcaggcg gtgttccagg ttgcgcagga atacataggc
      241 cgcatccagc gcagccacat cctgcaatga catctgcgcg ttttcagcta accgttgcag
      301 cacgctgcgg gtcgggcgga tgcgcagacg cgcatcgctg ccgccgcgta tcagctggaa
      361 cacctgtgcg gtgaattcga tctcgcgtat gcctcccggg ccgagcttga tgtgtccggc
      421 gcgatcgcgc cgcgcgacct cggcgcggat ctgcgcgtgc agcgagcgca tcgccgcaaa
      481 cgcgccgaaa tcgaggtact tgcggaacac gaacggccgc gcgaccgcgc ccagctgctg
      541 cgccgaagtg ttctcatcac ccgcgccctt gctgcgcgca gcaggaatgc cggcgtcacc
      601 gcttgcggca acgctcgcgt tgctgctcgt agcaagtgcg ctggggtcgc cacttgtggc
      661 gacgacgcgc gccttgatcc aggcatagcg ttcccattcg cgcccttcgg cgatgaagta
      721 gttttccagc gcgtccaggc tgcaggcgag cggtccggaa tcgccgttgg ggcgcaggcg
      781 catgtcgacg cggaatacct ggccgtcctc ggtgatatcg gcgagcacat tgatgagttt
      841 gcggccgaga cgggtgaaat attcgtggtt ggacaacagc cgcagtgccg cgccggcccc
      901 cgattggccc gcgctgccca cggtctcgcc ctcttccgga taaacgaaga tcagatcgat
      961 gtcggaggag acgttgagtt cgcgtccgcc gagcttgccc atgccgacca cgatcagctc
     1021 ctgtgccctg ccgttcgcgc tcggcgtgcc gtgctgtgcc cgcagccaga ggctcaagta
     1081 gccgagcgca tagtccaggc cgagttcggc caggtcgctc atgctgccga ccacctcggt
     1141 gagcggcgca cgtccgctca ggtcgcgcag catcacgcgc agcatgacct gctggcgcag
     1201 ttggcgcagc atgcgcttga gttcttcctc gtttgcgggc gcctggcgcg cgaggcaggc
     1261 gcgcatggca atggcatcga aaggctgttc gattgcatc
//
LOCUS       DASRVO010000749         1040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_176819, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000749 DASRVO010000000
VERSION     DASRVO010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176819"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFF05_09245"
     CDS             complement(<1..651)
                     /locus_tag="VFF05_09245"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HZW52035.1"
                     /translation="MKRLIVNADDFGMTPGVNRAVLEAQQCGILTSTTLMATGAAFEE
                     AVQLAVAAKTLDVGCHVVLLEGAALLPAAEIPSLAFRDSDGTTHFRRGFGAFIKAALA
                     GRLHGEDIYRETRAQITRLRESGIDVQHLDTHKHAHVFPSVFRPLLRAALDSGVRAVR
                     NPFEPIRAMSWDSVRRHRRLWSRYLPVRMLRAFANEFLAECRDLGIATPQGTVGITL"
     gene            complement(660..>1040)
                     /locus_tag="VFF05_09250"
     CDS             complement(660..>1040)
                     /locus_tag="VFF05_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52036.1"
                     /translation="LLAHRLLGLHFKDTQCGLKAFTREAAQSMFEHQSIDGWGFDPEL
                     LLIAGRFGYQMVEVPIELNHDYTTSRFRPVRDGITTFKELLAIVVRDAMGAYPRSARA
                     SNVASVPTRAAEVPSIAESGQEAA"
BASE COUNT          187 a          358 c          323 g          172 t
ORIGIN      
        1 cagcgtgatc ccgaccgtcc cctggggcgt ggcgatgccg agatcgcggc actccgccag
       61 gaactcgttg gcgaaggccc gcagcatgcg caccggcagg tagcgggacc agagccgtcg
      121 atgccgccgg acgctgtccc acgacatggc ccgaatcggc tcaaacggat tgcggaccgc
      181 gcgcacaccg gaatccagcg ccgcgcgcaa cagtggccgg aagacgctcg gaaacacgtg
      241 cgcgtgcttg tgcgtatcga ggtgctgcac gtcaatgccg gactctcgca ggcgcgtaat
      301 ttgcgcgcgc gtctcgcggt agatgtcctc accgtgcaac cgcccggcca gcgcggcctt
      361 gatgaacgcg ccaaatccgc ggcggaagtg ggtggtcccg tccgagtcac ggaatgccag
      421 cgagggaatt tccgccgccg gaagcagcgc tgccccttcc agcagcacca cgtggcagcc
      481 gacgtcgagg gtcttggcgg caactgcgag ctgcacggcc tcctcgaagg ccgctcccgt
      541 cgccatcagc gtggtggagg tcaggatgcc gcactgctgc gcctccagca cggcacggtt
      601 cactccgggc gtcatcccga agtcgtcggc atttacgatc aggcgcttca tgcgatttcc
      661 taagccgcct cctgtccgga ttccgcgatc gacggcactt ccgcggctcg cgtcggcaca
      721 gaggcaacgt tgctggcgcg cgcgctgcgt gggtaagccc ccatcgcatc gcgcacaacg
      781 atcgccaaca attccttgaa cgtcgtaatg ccgtcccgta ccgggcggaa tcgtgaggtc
      841 gtgtaatcgt gattcagctc gatcggcact tccaccatct ggtatccaaa gcggcccgcg
      901 atcagcagca actccggatc gaatccccag ccgtcgatac tctggtgctc gaacatggat
      961 tgcgccgcct cgcgtgtgaa cgccttcaac ccgcactgcg tgtccttgaa atgcaacccg
     1021 agcaggcgat gcgccaacag
//
LOCUS       DASRVO010000750         1424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_255684, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000750 DASRVO010000000
VERSION     DASRVO010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255684"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFF05_09255"
     CDS             <1..601
                     /locus_tag="VFF05_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52037.1"
                     /translation="PYSKSYVRHLDFYYAQYHPDEDFVETFAVWLTPGLDWRGKYAGW
                     KALDKLLFVDKLMAGIAGKPPLRATGRQLWKAATIRSTLARYYQKRREAEAEDFPEFH
                     DDNLRRLFREGMPKGETRRSVAKLLQAHRKELLVAVSNWTGERRYMVSKVYKAVLQRS
                     RALRLVTEDADTVAMLKLTTYLTTLMMNYRYTTRLRGKL"
     gene            619..>1424
                     /locus_tag="VFF05_09260"
     CDS             619..>1424
                     /locus_tag="VFF05_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52038.1"
                     /translation="MVFDLPYAVKPDYDFAAEFADPDGSYTENDVWRALLECGYEVHR
                     LSLFDDVRPLLDAVREIKPDVIFNLCETFHTVTHWDKNIAATMELLGVPVTGASASAL
                     FLCNDKGLCKKILRFHRVRVPRFHTFYRGHKVWLPKALRLPCIIKPLTEEASRGISQA
                     SVIDDEAGFLARIQMIHERMNLDAIAEEYIDGRELYVSVIGDKALRILPAREMTFGQM
                     AEDEPRIATYKAKWDDAYRERWGIKNHFAKPLEPELQARIDDVCKRAYRAL"
BASE COUNT          282 a          439 c          457 g          246 t
ORIGIN      
        1 tccgtacagc aagagctatg tgcggcatct ggatttctac tacgcccagt atcacccgga
       61 cgaggatttc gtcgaaacct tcgcggtctg gctgacgccg ggcctggact ggcgcgggaa
      121 atacgccggc tggaaggcgc tcgacaagct gctgttcgtc gacaagctga tggcgggcat
      181 cgccggcaag ccgccgctgc gcgcgaccgg ccgccagttg tggaaggcgg cgacgatccg
      241 cagcacgctg gcgcgctatt accagaaacg ccgtgaggcc gaggccgagg actttccgga
      301 attccacgac gacaacctgc gccgcctgtt tcgcgaaggc atgccaaagg gcgagacgcg
      361 gcgatcggtc gcgaagctgt tgcaggcaca tcgcaaggaa ctgttggtcg cggtgtcgaa
      421 ctggaccggc gagcggcgct acatggtcag caaggtatac aaggcggtac tgcagcgcag
      481 tcgcgcgttg cgtctcgtca ccgaggatgc ggatacggtg gcgatgctca agctcacgac
      541 gtatctgacg acgctgatga tgaattatcg ctacacgacc cgcctgcggg ggaagctgtg
      601 atgggaacac gcgtgctgat ggtcttcgac ctgccgtatg cggtgaagcc cgactacgac
      661 ttcgccgctg aattcgccga tccggacggc agctacaccg aaaacgatgt ctggcgcgcg
      721 ctgctcgaat gcggttacga ggtgcatcgc ctcagcctgt tcgacgacgt gcggcccttg
      781 ctcgacgccg tgcgcgaaat caagcccgac gtgatcttca acctgtgcga aaccttccac
      841 acggtcacgc actgggacaa gaacatcgcc gcgacgatgg agctgctcgg cgtgccggtc
      901 accggcgcaa gcgcgagcgc gctgttcctg tgcaacgaca agggactgtg caagaagatc
      961 ctgcgctttc atcgcgtgcg cgtgccgcgt ttccatacgt tctaccgcgg ccacaaggtg
     1021 tggctgccga aggcgctgag actgccgtgc atcatcaagc cgttgaccga ggaagcctcg
     1081 cgcggcattt cgcaggcttc cgtcatcgac gacgaggcgg gttttcttgc gcgcatccag
     1141 atgatccacg agcgcatgaa cctcgacgcc atcgccgagg aatacatcga cgggcgcgaa
     1201 ctctatgtca gtgtcatcgg cgacaaggcg ctgcgcatcc tgccggcgcg cgagatgacg
     1261 ttcggccaga tggccgagga cgaaccacgc atcgccacct acaaggccaa gtgggacgac
     1321 gcctatcgcg agcgctgggg catcaagaat catttcgcca agccgctgga acccgagttg
     1381 caggcgcgca tcgacgacgt gtgcaagcgc gcctatcgtg cgct
//
LOCUS       DASRVO010000751         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_74390, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000751 DASRVO010000000
VERSION     DASRVO010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74390"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFF05_09265"
     CDS             complement(<1..283)
                     /locus_tag="VFF05_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HZW52039.1"
                     /translation="MRCGRFASGGGTNIGIPVALGNAMKQSVFVTRALIALCAAGMMF
                     QASLARAAMIGPEAALPAPSQVEQDRAKVQAFLERANVKERMQAMGVSGV"
     gene            complement(340..522)
                     /locus_tag="VFF05_09270"
     CDS             complement(340..522)
                     /locus_tag="VFF05_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52040.1"
                     /translation="MELNGKPAALARIFCAAAMLSTLGGMLGCSGSNVGPEPPWNLSA
                     QQAPTDGGPAKQHENH"
     gene            complement(767..>1188)
                     /locus_tag="VFF05_09275"
     CDS             complement(767..>1188)
                     /locus_tag="VFF05_09275"
                     /inference="COORDINATES: protein motif:HMM:NF027449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HZW52041.1"
                     /translation="HTDEPALFSGDTLFNAGAGNVHNGGNVNELFATFVNQLANLPDN
                     TRVYPGHDYIENNLRFTLAREPSNAEAQAMLPQVAGHDPATSAVTTLADEKRINTFMR
                     LDSRGVIDRLRADYPELAAQPDARTVFTRLRELRNKW"
BASE COUNT          188 a          386 c          390 g          224 t
ORIGIN      
        1 cgacgccgct cacgcccatc gcctgcatcc gttccttgac gttggcgcgt tccaggaaag
       61 cctgtacctt ggcgcggtcc tgttcgacct ggcttggcgc gggcagggcc gcttccggcc
      121 cgatcatcgc ggcacgagcg agcgaagcct ggaacatcat cccggcggca cagagcgcga
      181 tcagagcgcg cgtgacgaag acactttgtt tcatcgcatt ccctagagca acaggaatgc
      241 cgatattagt gccgccgccg gacgcaaaac gtccgcatcg cacaacgcgg cgtgtaagag
      301 atggttgctc ggccgtgccg gttgcggcag caaaggctct cagtggttct cgtgctgctt
      361 cgccggcccg ccgtcggtgg gcgcctgctg ggccgacagg ttccatggcg gctcgggccc
      421 gacattgctg ccgctgcacc ccagcatgcc gcccagcgtc gagagcatcg ccgcagcgca
      481 aaagatgcgc gcgagtgccg ccggtttgcc gttcaattcc atgtctccac ctcgaactca
      541 gcagtaagtt gggcattgtg gagctcattg ccgggccgca aacgtcacga tctgcaaagc
      601 atgacgccaa gtgcgtcggc gtatcctgct cgtcgtcccc gcggaggcgg ggatcagcgc
      661 atcctgaaag ccctggattc ccgcctgcgc gggaatgacg atgtttcctg cgcctgcgcg
      721 ggaatgacga tctttgctgc gctcgcgctc cgcagtgcga gccgcctcac cacttgttgc
      781 gcagctcccg caaccgggtg aacaccgtcc tcgcgtccgg ctgcgccgcc aattcgggat
      841 agtccgcgcg caagcggtcg atcacgccgc ggctgtccag ccgcatgaag gtgttgatgc
      901 gcttctcgtc ggcgagcgtg gtcacggcag aggtggcggg atcgtggccg gccacttgcg
      961 gcagcatcgc ctgggcttcg gcattgctcg gctcgcgcgc cagcgtgaag cgcaggttgt
     1021 tctcgatgta gtcatgcccc ggataaacgc gcgtgttgtc gggcagattg gcgagctggt
     1081 tgacgaacgt ggcgaagagt tcgttcacgt tgccgccgtt gtgcacgttg ccggcgcccg
     1141 cattgaacag cgtgtcgccc gagaacagcg ccggctcatc ggtgtggg
//
LOCUS       DASRVO010000752         1909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_74410, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000752 DASRVO010000000
VERSION     DASRVO010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74410"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFF05_09280"
     CDS             complement(<1..204)
                     /locus_tag="VFF05_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52042.1"
                     /translation="MQIQPILAALRKHKLAAVLIALEIALACAVLCNACFMIADRIAM
                     MGIDSGVDEGALATMQLTGYDAAK"
     gene            complement(216..1535)
                     /locus_tag="VFF05_09285"
     CDS             complement(216..1535)
                     /locus_tag="VFF05_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW52043.1"
                     /translation="MFAYYLDLALRSLKRNRVLTALMVLAIAVGIGASMTTLTVMHLL
                     SGDPLPGRSAHIFYPQVDASPAGFTRHGPLDMLDYRSAMDLWSAHRADRQALIVNSQI
                     KVRAPDANLPPLMANMLSTTADFFPMFDVPLRYGNGWSAQDDGDRARVAVISAELNDK
                     LFRGENSVGRILRLKDADVRIVGVLAPWRPSPLFYDVRGGRFANGDTADFYGRPEDVL
                     TPVSSGLEINAGNFWQFTCWHPPETPGHLENAGCVWVALWVQLDDAAKLSAYRAFLDH
                     YAQEQKTLGRFASTQTRLPSLTQWLDFNGVVPADVKLQTGLAFAFLAICLFNTIGLLL
                     AKFLRRSGEIGVRRALGASRRAIFAQCLVEAGMIGLLGGFGGWLLTLGGLWLARRQAV
                     AYADLAHLDLGMFATTFAAAIAVSLLAGGLPALRASRIAPALQLKTL"
     gene            complement(1535..>1909)
                     /locus_tag="VFF05_09290"
     CDS             complement(1535..>1909)
                     /locus_tag="VFF05_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZW52044.1"
                     /translation="ILMGVICLILLAVTGAGIVGLTSFWVGQRHKQIGVRRALGARKL
                     DILRYFQAENLLIAGGGALAGIVLAIGLNLWLMHRFEMDRIPVVYVLLGVAVVLALGQ
                     LAVFAPARRAANVPPVVATRSV"
BASE COUNT          362 a          644 c          619 g          284 t
ORIGIN      
        1 cttggcggcg tcatagccgg tcagctgcat cgtcgccagc gcgccttcgt ccacgccgct
       61 gtcgatgccc atcatcgcga tacggtcggc gatcatgaaa caggcattgc acagcactgc
      121 gcaggcgagc gcgatctcca acgcgatcag cacggcggca agtttgtgct tgcgcagcgc
      181 ggcgagaatg ggctggattt gcacgtcgga aatcctcaca gtgttttcaa ttgcagcgcc
      241 ggcgcgatgc gactggcgcg cagtgccggc aatccgccgg cgagcaggct gaccgcgatc
      301 gccgccgcga acgtggtcgc gaacatgccc aggtccagat gcgccaagtc cgcataggcg
      361 accgcctgcc ggcgcgccag ccacaagccg cccagcgtca gcagccagcc gccgaatccg
      421 ccaagcaagc cgatcattcc ggcttcgacc aggcattgcg cgaagatcgc acggcgcgac
      481 gcgcccagcg cgcggcgcac gccgatctcg ccgctgcggc gcaggaattt cgccagcagc
      541 aatccgatcg tgttgaatag acaaatcgcc aggaacgcaa acgcgagtcc agtttgcagc
      601 ttcacgtccg ccggcaccac gccgttgaaa tccagccact gcgtgagcga tggcagccgc
      661 gtttgcgtgc tggcgaaacg gccgagcgtt ttctgttcct gcgcataatg atcgaggaac
      721 gcgcgatagg cggacagctt cgccgcatcg tccaactgca cccacagcgc cacccagacg
      781 cagcccgcgt tttcgagatg accgggcgtt tccggcggat gccagcaggt gaactgccag
      841 aaattgcccg cgttgatttc caggccactg gaaaccggcg tgagcacgtc ttcaggcctg
      901 ccgtagaaat ccgcggtgtc gccattggcg aagcggccgc cgcgcacgtc gtagaacagc
      961 ggcgagggcc gccacggcgc caacacgccg acgatgcgca cgtcggcatc cttcaagcgc
     1021 aagatgcggc cgacgctgtt ctcgccgcga aacagcttgt cgttcagctc cgccgaaatc
     1081 accgcgacgc gcgcgcgatc gccatcgtcc tgcgcgctcc agccgttgcc gtagcgcagc
     1141 ggcacgtcga acatcgggaa aaagtccgcc gtggtggaca gcatgttcgc catcagcggc
     1201 ggaaggttcg cgtcgggcgc acgcaccttg atctggctgt tgacgatcag ggcctggcgg
     1261 tcggcgcgat gcgcactcca caaatccatt gccgagcggt aatcgagcat gtccagcgga
     1321 ccgtgccggg tgaatcccgc gggactggcg tccacctgcg gatagaagat gtgcgcactg
     1381 cgccctggca gcgggtcgcc ggagagcagg tgcatcacgg tcagcgtcgt catgctcgcg
     1441 ccaatgccga ccgcgatggc cagcaccatc agcgcggtca gcacgcggtt gcgcttgagg
     1501 ctgcgcaagg cgagatcgag gtagtaggcg aacatcacac gctccgcgtc gccaccaccg
     1561 gcggcacgtt cgccgcgcgc cgcgcgggcg cgaacacggc gagctggccg agcgccagca
     1621 cgacggcaac gccgagcaac acgtacacca cggggatacg gtccatttcg aagcggtgca
     1681 tcagccacag gttgaggccg atcgcgagca cgattccagc gagcgcgccg ccgccggcga
     1741 tgagcaggtt ctcggcctgg aagtagcgca ggatgtcgag cttgcgtgcg ccgagtgcac
     1801 gacgcacgcc gatctgcttg tggcgctggc cgacccagaa gctggtcagt cccacgatgc
     1861 cggcgccggt gaccgcgagc aggatcaggc agatcacgcc catcaggat
//
LOCUS       DASRVO010000753         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_74424, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000753 DASRVO010000000
VERSION     DASRVO010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74424"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..586)
                     /locus_tag="VFF05_09295"
     CDS             complement(11..586)
                     /locus_tag="VFF05_09295"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZW52045.1"
                     /translation="MKRALKQADFDRPSDEMAQALIGATLLVDDIGGRIVETEAYDRT
                     DPASHSFSGPTARNQSMFGPPGRAYVYRSYGIHWCLNFVCREEGHGAGVLIRAIEPMY
                     GIEVMQQRRGVADIRLLCSGPGRVGQALAITRDLNGQRLDRPPFRVLAAQGRFDIVTG
                     PRIGISKAMEIHWRFGLAGSRFLSRPFARAR"
     gene            731..1477
                     /gene="imuA"
                     /locus_tag="VFF05_09300"
     CDS             731..1477
                     /gene="imuA"
                     /locus_tag="VFF05_09300"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HZW52046.1"
                     /translation="MSCVQELRKLDPSSLPHVWRVADLAPANATIATGHSRLDAALPG
                     SGWPTGCLIEILQQTPARHVWQLLLPALVDIMRRKAGPAVLVGSPLQPFGPSLSAQGL
                     PAERLLCVDAEQPRLRLWAAEQALRCAQVAAVLAWLPAAKNEELRRLHLAAKQHQRLL
                     FVFRRVQSRDEASPATLRLQVQGVDCIEIEVVKRRGPPLLEPLILPATSQRVRALLQA
                     RRASRTGVPAANEIAASGRSHVLDRTATVE"
     gene            1509..>1812
                     /locus_tag="VFF05_09305"
     CDS             1509..>1812
                     /locus_tag="VFF05_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52047.1"
                     /translation="MQFTPRVAKVEEALLLEASASLRLWGGRRRLSHQLFAQCEPLAP
                     ARWSEAATALKALGLLRMQRDAETAPRCRTQDMPLAMLSAAREHLESLERIGCRTWG"
BASE COUNT          292 a          588 c          627 g          305 t
ORIGIN      
        1 cgcatgaatc tcacctggcg cgtgcgaacg ggcggctgag aaagcgcgaa ccggccagtc
       61 cgaagcgcca gtggatctcc atcgccttcg agatgccgat gcgcggcccg gtgacgatgt
      121 cgaaccggcc ttgcgcagcg agcacgcgaa agggcggccg atccagccgc tggccattga
      181 gatcgcgtgt gatcgccagc gcctggccga cgcggccggg tccggagcac agcagacgga
      241 tatccgcgac gccacgacgc tgctgcatca cttcaatccc gtacatcggc tcgatcgccc
      301 ggatcaggac gccggcgcca tgaccttcct cgcggcacac gaaattgaga caccagtgga
      361 tcccgtacga gcgatacacg taggctcggc cgggtgggcc gaacatcgac tggttgcgcg
      421 ccgtcggtcc ggaaaaactg tgggatgcgg gatcggtccg gtcataggct tcggtttcga
      481 caatccgccc gccgatgtca tcgacgagca gcgtggcccc gatcagcgcc tgagccattt
      541 cgtcggaggg ccgatcgaaa tcggcctgtt tgagggcgcg cttcactcgt gcgcccgcca
      601 acgacggtgg ccgacttcga atccgggaag aagtccgcgc ctgtgggttt ttccgccagg
      661 cggcagcagg gcgatggccg cgctccagcc agaaatactg tataaatgaa cagtattgcc
      721 cgtgtcagtc atgtcttgcg ttcaagaact gcggaagctc gatccctcga gcctgcctca
      781 tgtgtggcgg gtggctgatc ttgcgccggc aaacgccacg attgcgacag gtcattcgcg
      841 gctggatgcc gcattgcctg ggagtggctg gcccacgggg tgtctgatcg aaatcctgca
      901 gcaaacgcct gctcggcatg tgtggcaatt attgctgccc gcgctcgtcg acatcatgcg
      961 ccgcaaggcc ggcccggccg tcctggtggg ctcgcctttg cagccgttcg gtcccagcct
     1021 gagcgcgcag ggattgccgg cggagcgcct gctgtgcgtt gatgccgaac agccgaggct
     1081 gcgcttgtgg gcggcggagc aggccttgcg ctgcgcacag gtcgcggccg tgctggcgtg
     1141 gctgccggcc gcgaagaacg aggagttgcg ccgtctgcat cttgcggcca agcagcacca
     1201 gcgcctgctg ttcgtcttca ggcgcgtgca aagccgcgac gaggcatcgc cggcgacgct
     1261 gcgcttgcag gtgcagggcg tggattgcat cgagatcgag gtcgtcaagc ggcgcgggcc
     1321 gccgctgctc gaaccgttga tcctgcccgc gacttcgcag cgcgtgcgcg cattgctgca
     1381 ggcacgccgc gccagccgca ccggcgtgcc tgccgcgaac gagatcgcgg cttcagggag
     1441 atcgcatgta ctggatcgca ctgcaaccgt cgaatgagca ggagcgcact gcctgggcgt
     1501 ggcgcgcgtt gcagttcacg ccgcgcgtcg ccaaggtgga agaggcgctg ttgctcgaag
     1561 cgtccgcatc gctgcgcttg tggggcgggc gccggcggct gtcacaccag ctctttgcgc
     1621 aatgcgagcc gctggcgccg gcgcgctggt ccgaggccgc gaccgcgctg aaggcgctcg
     1681 gcctgcttcg aatgcagcgc gacgccgaaa ctgcgccgcg ttgccgcacg caggatatgc
     1741 cgctcgccat gctgagtgcc gcgcgcgaac acctggaatc cctggagcgg atcggctgcc
     1801 gcacctgggg cg
//
LOCUS       DASRVO010000754         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_176905, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000754 DASRVO010000000
VERSION     DASRVO010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176905"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VFF05_09310"
     CDS             <1..630
                     /locus_tag="VFF05_09310"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZW52048.1"
                     /translation="PRIGALSGPSFAKEVAMSDPTAVTVGSHDTDLATTVQREFSDHT
                     FRIYTNDDIVGVELGGALKNVIAIAAGVCDGLGLGYNTIAALITRGLAEIARLAVACG
                     ARQETLAGLAGMGDLVLTCTGGLSRNRTVGVELGKGRPLDDIIAGMHGMVAEGVLTTN
                     AAVGLARKLRVEMPITEQMHAILHEGKPPRQAIVELMTRPGTSEFGVAR"
     gene            813..1340
                     /locus_tag="VFF05_09315"
     CDS             813..1340
                     /locus_tag="VFF05_09315"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HZW52049.1"
                     /translation="MPASPPPTRARDFLTLAGLGLGMLGIFMMASGAYVLWNRYSVTK
                     DWTAAEAEVVSGTLYSRTSKNEAAIPAAKMWGAHFLVRYHAGNATIDSLADMGYLSAS
                     QAEMEHWLHVFQPGSHHTVRYDPFQPGRVVLTGNHVWQTYPGPARIFVAGVALMVIAA
                     VLIRLARRPAPQPVS"
     gene            1483..>2248
                     /locus_tag="VFF05_09320"
     CDS             1483..>2248
                     /locus_tag="VFF05_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZW52050.1"
                     /translation="MRKALLLYNPHSGRQRARREADVELAVSEMRAAGVDAVAEPTRA
                     AGSAGEQARAAAGFDTIIACGGDGTVHDVLQGVAGTPLQLAVLPLGTGNALANDLGIA
                     RDVRTAVRMLLDAAAQPISIGRVTTTRNGAPETRSFMIGVGVGADAHMVYRLSAAFKR
                     RFGMIAYYTESTRQWATHSFPEFEVEYRDLETGETRRGPATQVLAIRITYFGGMLRRL
                     APGAALQRNDLRLVIFRTRSRLRYLRYMWNVLCDREQ"
BASE COUNT          393 a          775 c          690 g          390 t
ORIGIN      
        1 ccgcgcatcg gcgcgctcag cggaccgtcg ttcgccaagg aagtcgcgat gtccgacccg
       61 acggccgtga ccgtcggcag ccacgacacc gacctcgcga caacggtaca gcgcgaattc
      121 agcgatcaca ccttccggat ctacaccaac gacgatattg tcggcgtcga actcgggggc
      181 gcactgaaga acgtgatcgc catcgcggcg ggcgtctgcg atggcctcgg cctcggctac
      241 aacaccatcg ccgcactcat cactcgcggg ttagccgaga tcgcccgcct tgccgtcgcg
      301 tgcggcgcac gccaggagac cctagccgga ctcgccggca tgggcgacct cgtcctcact
      361 tgcaccggag gcctctcgcg caaccgtacc gtgggcgtcg aactcggcaa ggggcggccg
      421 ctcgacgaca tcatcgccgg catgcacggc atggttgccg aaggcgtgct caccaccaat
      481 gccgccgtcg gcctggcgcg aaagctcagg gtcgagatgc ccatcaccga gcagatgcac
      541 gccatcctgc acgagggcaa gccgccgcgg caggccatcg tcgagctgat gacccgcccg
      601 ggtaccagcg agttcggcgt ggcgcgatag ttcccagttc tcagttctca gtcaaaacaa
      661 aaccgctgat ctcgctgatg aacgctgatt tttctttggc tttttcttct atccgcgtga
      721 atcagcgaaa tcagcggttt cttttctggt caattcggca attcctcagt tcggcaattc
      781 ggcaatttct tcgttctcta taatcgaagg cgatgcccgc ttcgccgccg ccaacccgcg
      841 cccgcgactt cctgaccctc gctggcctgg gcctcggcat gctggggatt ttcatgatgg
      901 cgtcgggagc gtacgtcctg tggaaccgct acagcgtcac caaggactgg acggcagcgg
      961 aggcggaagt cgtctccggc accttgtatt cgcggacgtc gaagaacgaa gccgccatcc
     1021 cggccgcgaa gatgtggggc gcgcacttcc tggtgcgcta ccacgcgggc aacgccacca
     1081 ttgattctct ggccgacatg ggatacctca gcgcgtcgca ggcggaaatg gaacactggc
     1141 tgcacgtctt ccagcccggc tcgcaccaca ccgtccgcta cgatccgttt cagcccggcc
     1201 gggtggtgct gaccggcaac cacgtttggc agacctatcc cggcccggcg cgcatcttcg
     1261 tcgccggcgt ggcgctgatg gtgatcgcgg cggtgttgat ccggctggcg cggcggcccg
     1321 caccgcagcc cgtttcttaa ccacagcgga acacagagga attcttgttt ttctctgtga
     1381 gtctcggtgc cctctgtggt taagaaaaag aggctccctc ccactaaagg tatgatgttc
     1441 ccccgcagcg ccagccgcga agagcgaacg gcgaaccgca acatgcgcaa ggctctgctt
     1501 ctctacaacc cgcattcggg aaggcagcga gcgcggcgcg aagccgacgt ggagctggcc
     1561 gtctccgaaa tgcgcgccgc gggcgtggac gcagttgccg aaccgacgcg cgccgcagga
     1621 tcggccggcg agcaggcgcg cgcggccgcc gggttcgaca ccatcatcgc ctgcggcggc
     1681 gacggcaccg tacacgatgt gctgcaaggc gtcgccggca cgccgctgca actcgccgta
     1741 cttcccctgg gcaccggcaa cgcgctggcc aacgatctcg gcatcgcacg cgacgtgcgg
     1801 accgccgttc gcatgctgct cgacgcagcg gcgcagccaa tctccatcgg gcgcgtgacc
     1861 accacccgca acggcgcgcc cgaaacccgc agtttcatga tcggcgtggg cgtgggcgcc
     1921 gatgcgcata tggtctatcg tcttagcgcc gccttcaaac ggcgtttcgg catgatcgcc
     1981 tactacacgg aatcaacgcg gcagtgggcc acgcacagct ttcccgaatt cgaggtggaa
     2041 taccgcgacc tggaaaccgg cgagacgcgc cgcggcccgg ccacgcaggt gctggccatt
     2101 cggattacct acttcggcgg catgctgcgg cgtttggccc ccggcgccgc cctgcagcgc
     2161 aacgaccttc gcctggtgat cttccgtacc cgcagccgtt tgcgctacct gcgctacatg
     2221 tggaacgtgc tgtgcgaccg cgagcaga
//
LOCUS       DASRVO010000755         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_176927, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000755 DASRVO010000000
VERSION     DASRVO010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176927"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..443
                     /locus_tag="VFF05_09325"
     CDS             213..443
                     /locus_tag="VFF05_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52051.1"
                     /translation="MPNRRAVLFRWGALEAEQRLGEGSFGEVYRAFDPWLGRHVALKL
                     FRDKRNGGGRNAGNAGRDDSSATTALTRPFAP"
     gene            complement(421..867)
                     /locus_tag="VFF05_09330"
     CDS             complement(421..867)
                     /locus_tag="VFF05_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZW52052.1"
                     /translation="MLANLTLHYGWWLLALVLIGAELMVPGFFMLWIGIAAAALGVVL
                     IFVPQMSFGMQAVVFAVFAVISCYLYWHFVRHAMHERSDQPNLNRRAEQYIGRRFVLE
                     TAIVNGQGKARVGDSLWLAEGPDLPAGAPVEVVGVDGSTLKVQKAG"
     gene            complement(869..1810)
                     /locus_tag="VFF05_09335"
     CDS             complement(869..1810)
                     /locus_tag="VFF05_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HZW52053.1"
                     /translation="MNGGFVALVLLVVVAIFVSKTVRVVPQGYEWTKETFGRYTSTME
                     PGLHILIPIYHTVGRKMNMMEQVLDVPSQDVITKDNAVVRVDGVVFYQVLDAAKAAYE
                     VANLQQAILNLVMTNIRTVLGSMDLDESLSKRDEINARLLKVVDEATHPWGLKCNRIE
                     IKDIQPPRDLVDSMARQMKAEREKRANILEAEGFRQAAILKAEGEKQSVILAAEGKRE
                     AAFREAEARERQAQAEAKATELVSQSIRAGDINAINYFVATKYVDALKAMVGAPNQKI
                     MLLPMEATGVLGSLAGIAELAKNSLSEQQAAKPVAKA"
     gene            complement(1863..2663)
                     /locus_tag="VFF05_09340"
     CDS             complement(1863..2663)
                     /locus_tag="VFF05_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52054.1"
                     /translation="MLFAPAAAHADYASDYMAGLAALARGENAQAAQYLKKALDAQSE
                     PVQRIMIQGSARPYLPHHYLGMAQFKLGDCAAAQAEWNDPVNARMLGLLRQLRVKEAL
                     LRGQRQDLPDERQLRAQEEQLLGQCTPAAASTGKSTTPQSTQTADKPPAATSASAPAT
                     SAPSPKPAAVAPAGPPQSLVRAFDDLMAGRYASAAKLDADSLSGTRSRFQAYLLRSAA
                     RFALARSGDKGQLDAARRDAHAAQVLDKSAPDERVFSPAFRAFYAEAQ"
     gene            complement(2692..>3137)
                     /locus_tag="VFF05_09345"
     CDS             complement(2692..>3137)
                     /locus_tag="VFF05_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52055.1"
                     /translation="GKRLQAATSMDDFDAMAGFVKGRDTAWTGDPAWAEMLRDLPAWR
                     AQVVAAAQAQAAANAGELLLNATPWARVDAVADGSGRAMTLPDDSSTPLLLKLPAGTY
                     SVVFRQPLDGKSKQLTVSIEGQKRATLSASFAVPDAKEYFKHAGF"
BASE COUNT          517 a         1045 c         1016 g          559 t
ORIGIN      
        1 attgccaacc gcctcgccaa ccgccaggac tgatgccgtg ccgagtcatt cggatgccgc
       61 cgacaagatc gaaaacgcac tgcatgccat cgccgacggc aggccggtgc cgtggagcga
      121 tctggagaag tacctgggcg aatccggcga gctcgacatc ctgcacctgc tcgacgacgt
      181 cgccggccta ccgcggcgag gaaggcgcca ccgtgccgaa ccgtcgcgcg gtgctgttcc
      241 gctggggcgc gctggaagcc gagcaacgcc tgggcgaagg tagtttcggc gaggtctacc
      301 gcgcgttcga tccctggctc ggccgccatg tcgccctgaa attgttccgg gacaagcgca
      361 acggcggcgg caggaatgcc ggcaacgccg gccgagacga ttcgtcagcg actacggcgc
      421 ttacccggcc ttttgcacct tgagcgtgga accgtcgacg ccgacgacct cgaccggcgc
      481 cccggccgga aggtcgggac cctcggccag ccacagcgag tcgccgacgc gcgccttgcc
      541 ctgaccgttg acgatggcgg tttccagcac gaagcggcga ccgatgtatt gctcggcgcg
      601 acgattcagg tttggctggt cggaacgctc gtgcatcgcg tggcgcacga agtgccaata
      661 caggtagcag ctgatcaccg cgaacacggc gaacacgacc gcctgcatgc cgaacgacat
      721 ctgcggcacg aagatcagca ccacgcccag cgccgcggcg gcaatgccga tccagagcat
      781 gaagaatccc ggcaccatca attccgcgcc gatcagcacc agcgcgagca gccaccagcc
      841 gtagtgcaag gtgaggttcg cgagcatgtc aggccttcgc cacgggtttg gcggcctgtt
      901 gttcgctcaa gctgttcttg gccagttcgg cgatgccggc cagcgaaccg agcacgccgg
      961 tcgcttccat cggcaacagc atgattttct ggttcggcgc cccgaccatg gccttgagtg
     1021 catccacata tttggtcgcg acgaaataat tgatcgcatt gatgtcgcct gcgcggatcg
     1081 actgggacac gagttcggtc gccttcgctt cggcctgggc ctggcgctcg cgcgcctcgg
     1141 cttcgcggaa cgccgcctcg cgcttgcctt cggcggccag gatcaccgat tgcttctcgc
     1201 cctcggcttt caggatcgcg gcctggcgga agccttctgc ttcgaggata ttcgcgcgct
     1261 tctcgcgctc ggccttcatc tgccgcgcca tcgaatcgac gaggtcgcgc ggcggctgga
     1321 tgtccttgat ctcgatgcgg ttgcacttca ggccccacgg atgggtggcc tcgtcgacca
     1381 ctttcagcag gcgcgcgttg atctcgtcgc gcttggaaag ggattcgtcc aggtccatgg
     1441 aaccgagcac cgtgcggata ttggtcatga ccaggttgag gatggcctgt tgcaggttgg
     1501 cgacctcata cgccgccttg gccgcgtcca gcacctggta gaacaccacg ccgtccacgc
     1561 gcaccacggc gttgtccttg gtgatcacgt cctgcgaagg cacgtcgagc acctgttcca
     1621 tcatgttcat cttgcgaccg accgtgtggt agatcgggat caggatgtgc aggcccggtt
     1681 ccatcgtgct cgtgtagcgg ccgaaggttt ccttggtcca ctcgtaaccc tgcggcacca
     1741 cgcgcacggt ctttgagacg aaaatcgcca ccacgacgag caggaccaac gcgacgaatc
     1801 caccattcat gatcttctcc gctaaacgac agaacggatt cgcagtataa cccggcgctt
     1861 cttcactgcg cttcggcgta gaacgcgcgg aaggcgggcg agaacacgcg ctcgtccggg
     1921 gcggacttgt ccagcacctg cgccgcgtgc gcgtcgcgac gcgcggcatc gagctggccc
     1981 ttgtcgccgg aacgcgccag cgcgaagcgc gccgcgctgc gcagcaggta ggcctggaaa
     2041 cgtgagcgtg tgccgctcag ggaatctgca tcgagcttcg ccgcgctggc gtagcgccca
     2101 gccatcagat cgtcgaaggc gcgcacgagc gattgcggcg ggccggccgg tgcgacagcg
     2161 gcgggcttgg gcgaaggcgc agacgttgcc ggcgccgatg cggaagtcgc cgcaggtggc
     2221 ttgtccgccg tttgtgtact ttgcggcgtt gtggattttc cggtgctggc ggcagcgggc
     2281 gtgcattggc ccagcaactg ttcttcctgc gcgcgcagtt ggcgctcgtc gggcagatcc
     2341 tggcgttgac cgcgcagcaa cgcttccttc acgcgcaact ggcgcagcag cccaagcatg
     2401 cgcgcgttca ccggatcgtt ccattcggcc tgcgccgccg cgcagtcgcc gagcttgaac
     2461 tgcgccatgc cgaggtaatg atgcggcaga tacggtcgcg cactgccctg gatcatgatg
     2521 cgctgcacgg gctcggattg cgcatccagc gcctttttca ggtattgcgc cgcctgcgcg
     2581 ttttcgccgc gagcaagcgc ggccagaccg gccatgtagt cgctggcgta gtccgcatgc
     2641 gccgccgcgg gagcgaacag catcgcgcaa agcgcgaacg ggaatgaacg cctagaagcc
     2701 ggcatgcttg aaatactcct tcgcatccgg caccgcgaat gacgcggaca acgtggcgcg
     2761 tttttgtcct tcgatgctca ccgtcaactg cttgctcttg ccgtccagcg gctggcggaa
     2821 gaccacgctg taggtgccgg ccggcaattt cagcagcaag ggcgtgctgc tgtcgtcggg
     2881 cagtgtcatg gcgcgtccgc tgccgtcggc aacggcatcc acgcgcgccc acggcgtggc
     2941 attgagcagc aattcgccgg cattggcggc ggcctgcgcc tgcgccgccg cgacgacctg
     3001 cgcgcgccac gcgggcaggt cgcgcagcat ttccgcccag gccgggtccc cggtccaggc
     3061 cgtgtcgcgg cccttgacga agccagccat cgcgtcgaag tcatccatgc tcgtcgcggc
     3121 ctgcagacgt ttgccca
//
LOCUS       DASRVO010000756         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_99628, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000756 DASRVO010000000
VERSION     DASRVO010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99628"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1403
                     /locus_tag="VFF05_09350"
     CDS             <1..1403
                     /locus_tag="VFF05_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing diguanylate
                     cyclase"
                     /protein_id="HZW52056.1"
                     /translation="LLGIYGKALADLLEARRLFAEQELDEASSQILQDIGITYRRLGY
                     PDKAREYLNQAIAHEKRVGDTESLYISALQLGYADQETGHYESALATQQHALELANST
                     GDRDSMASANLAIASVLTDLRRYPEALSALQKAADGFAAVDDRANVGMLAYERGRALA
                     GSKDTNKALAEFATAEAAFRANGNRRYQEILYRAQATSLEAANQPAAALTAYKNFLAA
                     HDAVAKERANQQAQMLREQFDTDRAKMENLRLKGEQAMKDRQVEALKKVRRWQQLAMG
                     LLAILLGLLSLLAIRQLARLRNWKRMASVDALTGVANLRGAQQFANSAMRRAHAQNEP
                     LTVLAIDIDEFKRINDVHGHPTGDRVLREVARACADALRDGDLLGRIGGEEFLAILPG
                     TLVDHAVDVAERLRRRVETLEIADLPGSVRATISIGVAEMLPHERDIGTLIERADAAL
                     YRAKALGRNRVVSAGS"
BASE COUNT          276 a          481 c          441 g          209 t
ORIGIN      
        1 cgctgctcgg catctatggc aaggcgctgg cggacctgct cgaagcgcga cgtcttttcg
       61 ccgagcagga attggacgaa gcgtccagcc agatcctgca ggacatcggc atcacctacc
      121 ggcgcctggg ctatccggac aaggcgcgcg aatacctgaa ccaggccatt gcccacgaaa
      181 agcgcgtcgg cgacaccgag tcgctgtaca tatccgcgtt gcaactaggt tacgccgatc
      241 aggaaaccgg gcattacgaa tccgcgctcg ccacccagca gcacgccctc gaactggcca
      301 actcgaccgg cgatcgtgac agcatggctt cggcgaatct cgccatcgct tccgtgctga
      361 ccgacctgcg gcgctacccc gaagccctgt cggcgctgca aaaagccgcg gacgggttcg
      421 ctgccgtcga cgaccgcgcc aatgtcggca tgctcgccta cgagcgcggc cgtgccctgg
      481 caggatcgaa ggacacgaac aaggcgctgg ccgagttcgc caccgcagaa gcggcgtttc
      541 gcgccaacgg caaccggcgc tatcaggaaa tcctttaccg cgcccaagcc acgtcgctgg
      601 aggccgccaa ccaaccggcc gcggcgctga ccgcgtacaa gaattttctc gccgcgcacg
      661 acgcggtggc caaggaacgc gccaaccaac aggcacagat gctgcgcgaa cagttcgaca
      721 cggaccgggc caagatggaa aacctgcgtc tgaagggcga acaagccatg aaggaccgcc
      781 aggtcgaggc gctgaagaag gtgcgccgct ggcagcagtt ggcgatgggg ctgctcgcga
      841 tcctgctcgg cctgctctca ttgctggcga ttcgccaact ggcacgactg cgcaactgga
      901 aacgcatggc ttccgtcgac gcgctcaccg gcgtggccaa tctgcgcggc gcgcaacagt
      961 tcgccaattc tgcaatgcgg cgtgcgcatg cgcagaacga accgctgacc gtgctcgcca
     1021 tcgacatcga cgaattcaag cgcatcaacg atgtgcacgg acaccctacc ggcgaccgcg
     1081 tgctgcgcga agtcgcccgc gcctgcgccg atgcgctgcg cgacggcgac ctgctcggcc
     1141 gtatcggcgg cgaggaattc cttgcgattc tgcccggcac cctggtcgat cacgccgtcg
     1201 acgtggccga gcgcctgcgc cggcgcgtcg aaacgcttga aatcgccgac ttgccgggca
     1261 gcgtgcgtgc caccatcagc attggcgtcg ccgaaatgct gccgcacgag cgcgacatcg
     1321 gcacattgat cgaacgtgcc gacgcggccc tgtaccgggc caaggcactt ggacgcaatc
     1381 gggtggtgag cgcgggttcc taggagc
//
LOCUS       DASRVO010000757         1666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_255880, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000757 DASRVO010000000
VERSION     DASRVO010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255880"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFF05_09355"
     CDS             complement(<1..193)
                     /locus_tag="VFF05_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW52057.1"
                     /translation="MRLHWLELVLFSTYAELRAERARSYLGFLWWIIEPAMNMATYYL
                     VFAVVLKTGQPDYVPFLLIG"
     gene            complement(200..616)
                     /locus_tag="VFF05_09360"
     CDS             complement(200..616)
                     /locus_tag="VFF05_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52058.1"
                     /translation="MSSIRLLAGLALALPGALFAAPLLPAASACGSQAQVSPDLRVKN
                     TARWTTASEENNFGYDVFRADAEKGPFVKLTKTPILGNGTTLETHHYAFADDAIDPCK
                     DYWYYVESIATDGSREKFTPVFHVPAKRHPSVPKRP"
     gene            complement(699..>1666)
                     /locus_tag="VFF05_09365"
     CDS             complement(699..>1666)
                     /locus_tag="VFF05_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW52059.1"
                     /translation="VTWEVPHRGAVDLTLHVAEDARLLAAQRDVFRRSLFEYLGGLGA
                     LLLVLLLATLRWSLRPLRRVTDDLMRVERGERERLSTAYPRELAGLAVNLNRFIDGER
                     EHLGRYRNTLADLAHSLKTPLAVIRNELESGASGESMRWTVLEQAGRMDQIVAYQLAR
                     AVASGHSTFAAPIALQPHAEEIVASLEKVHASRNILCEFEIEAQARFYGEAGDLLELL
                     GNLLENAFKWARHQVLLSARALPAPGARRSGLEIVVEDDGPGIAPEDVERLLQRGVRG
                     DERVQGHGIGLSIVQDLLKAYRGRLEVTRSQSLGGARFTVRFDAA"
BASE COUNT          298 a          550 c          563 g          255 t
ORIGIN      
        1 ggccgatgag caggaacggc acgtagtccg gctggccggt cttgagcacc accgcgaaca
       61 cgaggtaata cgtggccatg ttcatggccg gctcgatgat ccaccacagg aacccgaggt
      121 acgaacgcgc gcgctcggcg cgcaactccg cgtaagtgga aaaaaggacc agctccagcc
      181 agtgcaggcg catgccgcgt cagggtcttt tcggcacgga ggggtggcgc ttggccggca
      241 cgtggaacac cggcgtgaac ttctcgcgac tgccgtcggt ggcgatggat tcgacgtagt
      301 accagtagtc cttgcacgga tcgatggcat cgtcggcgaa tgcgtagtgg tgcgtttcca
      361 gcgtggtgcc gttgccgagg atcggggtct tggtcaactt cacgaacgga cccttctcag
      421 catccgcgcg aaacacgtcg tagccgaagt tgttctcctc gctggcggta gtccagcgcg
      481 cggtgttctt gacccgcaaa tccggcgaaa cctgggcctg gctgccgcag gcgctggccg
      541 ctggcaacag cggcgccgcg aacagcgcgc ccggcagggc cagtgcaaga ccggcaagga
      601 gacgaatcga cgacatgaca cactccgaaa acgcattgca gcgatgccgc gcacgatagc
      661 ggcagcgccg tggcggcgca atcgcggcgt attgagaatc aagcagcgtc gaagcgcacg
      721 gtgaatctcg ccccgccgag cgactgcgag cgcgtcactt ccagccggcc gcgataggcc
      781 ttgagcagat cctgcacgat ggacaagccg atgccgtggc cctgtacgcg ctcgtcgccg
      841 cgcacgccgc gctgcaagag ccgctcgacg tcttccggcg cgatgccggg accgtcgtcc
      901 tcgaccacga tctcaagacc gctgcgccgc gcacccggcg cgggcagcgc gcgcgcgctg
      961 agcaacacct gatgccgcgc ccacttgaat gcattctcaa gaagattgcc gagcagttcg
     1021 agcaggtcgc cggcctcgcc gtagaagcgc gcctgcgctt cgatctcgaa ttcgcacaaa
     1081 atattgcgcg acgcgtggac tttttccagg ctggcgacga tttcctcggc gtgcggctgc
     1141 aacgcgatcg gcgcggcgaa cgtggaatgg ccggacgcca ccgcgcgcgc aagctggtag
     1201 gcgacgatct gatccatgcg cccggcctgt tcgagaacgg tccagcgcat cgactcgccg
     1261 ctggcgccgg attcgagttc gttgcggatc acggcgagcg gcgtcttcaa actgtgcgcg
     1321 aggtccgcga gcgtgttgcg atagcggccg aggtgctcgc gctcgccgtc gatgaagcga
     1381 ttgaggttca ccgccaggcc cgccagttcg cgcggatacg cggtggacag gcgctcgcgc
     1441 tcgccgcgct ccacccgcat cagatcgtcg gtgacgcggc gcagcgggcg caagctccag
     1501 cgcagggtcg ccagcagcag cacgagcagc aacgcgccga ggccgccaag gtattcgaac
     1561 aggctgcgcc ggaacacgtc gcgctgtgcg gcgagcaggc gcgcgtcttc ggccacgtgc
     1621 agggtgagat ccaccgcgcc gcggtgcggc acctcccagg tcacgc
//
LOCUS       DASRVO010000758         1463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_99707, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000758 DASRVO010000000
VERSION     DASRVO010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99707"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFF05_09370"
     CDS             <1..543
                     /locus_tag="VFF05_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZW52060.1"
                     /translation="LRDFLHGIAERVFPIGRLEYSTSGLLLLTNDGELANRLLQAHHL
                     PQTYQIKIKTRLTREEMDELSRATGARIAPARGGAAPWYEVTLSEARRDALRNRLFQS
                     QHPVEKMKRVGFAGIEMGSLPPGTHRPLSGAELAALKRAAQRGEIGEQRAPAAGSAPA
                     RGRGRRGNRNRRAFRGGRKT"
     gene            545..>1463
                     /locus_tag="VFF05_09375"
     CDS             545..>1463
                     /locus_tag="VFF05_09375"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZW52061.1"
                     /translation="MARAIHEYVPQWIREIAPYAPGKPIEEVERALKVSAIKLASNEN
                     PLGPSPLAMEAARKAVAGSNRYPDGSGFYLRQALAKKHDLPMEQIILGAGSTELIDLS
                     ARLLLRPENAGVTSYGSFPLYYIAIRAAGARLVETPLRDYAFDLEAIARGLTPEARIV
                     FLANPNNPTGTMFTADEFDAFLARVPSHVLVVLDEAYCDYIERADYSRSIELVRRDAN
                     LLVLRTFSKVYGLAGLRIAYGLGPAGLLEEMNKIRGPFNTSNVAQAAAVAALEDREHV
                     RRSIESNRAGLAQLAAGLGELGVRCIPSVA"
BASE COUNT          261 a          418 c          549 g          235 t
ORIGIN      
        1 ctgcgcgact tcctgcatgg aattgcggag cgcgtctttc cgattggccg gctggagtac
       61 agcacctcgg gacttctgct gttgaccaac gacggcgaac tggccaatcg tctgctgcaa
      121 gcgcatcatc tgccgcaaac gtatcagatc aaaatcaaga cgcggctgac gcgcgaggaa
      181 atggacgagc tgtcgcgcgc aacgggcgcg cgcattgcgc cggcaagagg cggcgcggcg
      241 ccatggtacg aagtgacgct gtcggaggcg cggcgcgacg cgctgcgcaa ccggctgttc
      301 cagtcgcaac atccggtgga gaaaatgaag cgcgtgggct tcgcgggaat cgagatgggt
      361 tcgctgccac cggggacgca ccggccgcta tcgggtgcgg agttggccgc tctgaaacgg
      421 gcggcgcagc gaggcgaaat tggggagcag cgtgcgccag ccgccggcag cgcgcctgca
      481 aggggacgcg ggcggcgagg gaacaggaac cggcgggcgt ttcgcggcgg gcgcaaaacc
      541 taagatggcg cgcgcgatcc acgaatacgt tccgcagtgg attcgcgaaa tcgcgccgta
      601 cgcgccgggc aagcccatcg aagaggtgga gcgcgcattg aaggtttcag cgatcaagct
      661 ggcgtcgaat gagaacccgc tggggccgtc gccgctggcc atggaggcgg cgcgcaaggc
      721 ggttgcgggg tcgaaccggt atccggacgg cagcgggttt tatctgcgcc aggcgctcgc
      781 gaaaaagcac gaccttccaa tggagcagat catcctgggc gcgggctcga cggaactgat
      841 cgacttgagc gcgcggctgc tgctgcggcc ggaaaacgcg ggagtgacat cgtacggatc
      901 gtttccgctg tactacatcg cgatacgcgc ggcgggagcg cggctggtcg aaacgccgct
      961 gcgggattac gcgttcgacc tggaggcgat cgcgcgcggc ctgacgccgg aggcgcgcat
     1021 cgtctttctg gccaatccga acaatcccac gggaacgatg ttcacggccg atgaattcga
     1081 cgcgttcttg gcgcgcgtgc cgtcgcacgt gctggtggtg ctggacgagg cctattgcga
     1141 ttacatcgag cgggcagatt attcgcgctc gatcgagctg gtgcggcgcg acgcaaacct
     1201 gctggtgctg cgcacgtttt cgaaggtgta cggcctggcg gggctgcgca tcgcgtacgg
     1261 gctggggccg gcggggctac tggaagagat gaacaagatt cgcgggccgt tcaacacgtc
     1321 gaatgtggcg caagcggcgg cggtggctgc gctggaggat cgcgagcacg tgcggcggtc
     1381 gattgagtcg aaccgagccg ggctggcgca actggcggcg ggccttgggg agttgggcgt
     1441 gcgttgcatt ccctcggtgg cga
//
LOCUS       DASRVO010000759         1412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_176995, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000759 DASRVO010000000
VERSION     DASRVO010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176995"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFF05_09380"
     CDS             <1..249
                     /locus_tag="VFF05_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HZW52062.1"
                     /translation="YPATSSWLIVAIGLYVLAGACWLPVIALQLRMRDLAACAAREGW
                     ALPSQYHRCMRWWFVLGWPAFAAVLATFWLMVAKPALW"
     gene            complement(394..1293)
                     /locus_tag="VFF05_09385"
     CDS             complement(394..1293)
                     /locus_tag="VFF05_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZW52063.1"
                     /translation="MTDNPLESRIEGFVAYLRIERRYSPATLANYSRSLRRLREFLAA
                     QGVTRWRQVRGEQIQALIAKSHRAGLSPGSLRDMLSAYRSFYRWLAREGEATTNPAIG
                     VRSPKAPRKLPQVLDVDEVGALLDFPADDPESVRDRALLELLYSSGLRVSELTSVRWR
                     NLDMAEGMIRVTGKGSKTRIVPVGGKAIAALQALREQDHPGADDPLVRGQLGKPLTPG
                     GVRARLKRRAKDQGVWKRVYPHLLRHSCASHVLESSGDLRAVQELLGHADIGTTQIYT
                     HLDFQHLAKVYDAAHPRARRRAK"
     gene            complement(1299..1409)
                     /locus_tag="VFF05_09390"
     CDS             complement(1299..1409)
                     /locus_tag="VFF05_09390"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HZW52064.1"
                     /translation="MLAIGSGDAHRFHPGMGTVFLKLIAETIAAAIARYS"
BASE COUNT          230 a          448 c          460 g          274 t
ORIGIN      
        1 tatccggcca ccagctcgtg gctaatcgtc gcgatcggcc tgtacgtgct ggccggtgcg
       61 tgctggctgc cggtcattgc gttgcagttg cgcatgcgcg atctcgccgc atgtgcggca
      121 cgcgagggct gggcgctgcc gtcccaatat caccgctgca tgcgctggtg gttcgtgctt
      181 ggctggcccg cgttcgccgc cgtgctggcg acgttctggt tgatggtggc gaagccagca
      241 ttgtggtagc gccctgcaaa ttttttcttg ccggcatgta cccaaagaca accgcatcgc
      301 tgcaatcctg cgcctgttcc cgtgccgaat tgggatccgg tttccgagct tgcacttcgg
      361 gtgcgacgcg catcgtacaa agacgtcaca atcctacttg gcgcgccgcc ttgcccttgg
      421 atgcgccgca tcgtaaacct tggccaggtg ctggaagtcg aggtgcgtgt agatctgcgt
      481 ggtgccgatg tcggcgtggc cgagcagttc ctgcacggcg cgcaggtcgc cggaggattc
      541 gagcacgtgg ctggcgcagg aatgccgcaa caggtgcgga tacacgcgtt tccacacgcc
      601 ttgatccttg gcgcggcgct tgaggcgcgc acgcacgcca cccggcgtca gcggtttgcc
      661 gagctgcccg cgcaccagcg gatcgtcggc gccgggatga tcctgctcgc gcaacgcctg
      721 cagcgcggcg atggccttgc cgccgaccgg cacgatgcgc gtcttgctgc ccttgccggt
      781 gacgcgaatc atgccttcgg ccatgtcgag gttgcgccag cgcaccgagg tcagttccga
      841 cacgcgcagg ccggatgagt ataaaagttc caaaagtgca cgatcgcgca cgctttccgg
      901 atcgtcggcc ggaaaatcca gcagcgcgcc gacttcgtcc acatcgagta cctgcggcag
      961 tttgcgcggc gccttgggcg aacgcacgcc gatcgcggga ttcgtcgtcg cctcgccttc
     1021 gcgcgcgagc cagcggtaga aacttcgata agccgagagc atgtcgcgca gtgaacccgg
     1081 cgacaatccc gcacgatgcg acttggcgat cagggcctgg atttgttcgc cgcgcacttg
     1141 ccgccagcgc gtcacgcctt gcgcggcgag gaattcgcgc aagcgccgca acgagcgtga
     1201 gtaattggcc agcgttgctg gtgaatagcg ccgttcgatg cgcaggtagg cgacgaagcc
     1261 ttcgatgcgc gattcgagcg gattgtcggt cattgcgttc aggaataacg ggcaatcgcc
     1321 gccgctatcg tttcggcgat cagtttcagg aacaccgtgc ccatgcccgg atgaaagcga
     1381 tgcgcatcgc cgctaccgat cgcaagcatg cc
//
LOCUS       DASRVO010000760         3985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_204797, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000760 DASRVO010000000
VERSION     DASRVO010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204797"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFF05_09395"
     CDS             <1..243
                     /locus_tag="VFF05_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZW52065.1"
                     /translation="ERVRHWLHLPVDAGRLRARLFAQGLVANERHVESGWEIEIDAPR
                     ALLEPLFGLPAGEGEWLRTQLAAADAASYNPSTATV"
     gene            251..1336
                     /gene="hflK"
                     /locus_tag="VFF05_09400"
     CDS             251..1336
                     /gene="hflK"
                     /locus_tag="VFF05_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZW52066.1"
                     /translation="MAWNEPGSGGGKRDPWQGKPPDLDAWLKRLRDGFNRLFRGGGGG
                     GGIGGGSGGIALIVLALVVGWLALDSWTVIDARQFGVVLRFGQFNRVMTSGLNLKWPA
                     PVERVIRVDATNVRSVSDEVRMLTKDENIVLVDFNVQYQVTDAEKYLFAANQPDETLK
                     QAAESAVRQVIGRSAMDTILSGHGSEVVGETKKLLQQTLDGYGVGLFVTEINFQKILP
                     PPEVKEAFDDANNAGNNQQQFINEAQGYAAKVVPLARGEAARIRAAAEGYKAAQIAVA
                     TGDTQRFSLLDTQYKAAPEVTRKRLYLETLQGVLANNTKIIDTGNGKNLIYLPLDKLK
                     SGLDATTAAAAASVADDTKKTGKEANQ"
     gene            1333..2223
                     /gene="hflC"
                     /locus_tag="VFF05_09405"
     CDS             1333..2223
                     /gene="hflC"
                     /locus_tag="VFF05_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZW52067.1"
                     /translation="MKLLPPIIALLLVLLIANSAFIVPAGQSALVLQFGRIEGSASTA
                     ADYKPGLHFKLPLVQQVVRYDRRILNLEAQPERYFTSEKKAVNVDFYVKWRISDPAAY
                     YRSFGADETQTLANQRLAPIIKDALRFEFTARTLNDLIANARSDITENVRKQANAATE
                     GKLGIRVVDVRIKRIEFPDEVLGSVYKRMSAERNRLANEQRSNGQEEAAKIEADADRQ
                     VQVIKAEAERDAQEARGEGDAKAAEIYAAAYAKDPDFYAFDRSLKAYREAFKGGSVIV
                     VDPKSEFMRYFDASKGEGGK"
     gene            2230..2415
                     /locus_tag="VFF05_09410"
     CDS             2230..2415
                     /locus_tag="VFF05_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HZW52068.1"
                     /translation="MPHAFAAALCLVLVLEGLFLFAAPRAWQQMAEQMRQLDPRQLRV
                     IGGIMIAAGLLLLKLVV"
     gene            2534..3280
                     /locus_tag="VFF05_09415"
     CDS             2534..3280
                     /locus_tag="VFF05_09415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZW52069.1"
                     /translation="MILRGILIAAATLAAGAAFAQNGGSTASASQAQAQQQTPPAPLT
                     AADKPNLSYAIGYQIGSDFVERKIDIDLNAVIRAIQDGYAKHAPTVSEDKMHDLLARM
                     QYQMYTKAKGEFDKLAGENKAKSDKFLADNKSKTGIHVLPSGVQYRVIEEGTGTQHPT
                     LNSEVTVHYRGSLMNGLEFDSSFARGEPVHFKVGDVIKGWQDVLPLMRVGDHWQIFVP
                     AALAYGERGQPPRIGPNEALVFELKLISIK"
     gene            complement(3395..>3985)
                     /locus_tag="VFF05_09420"
     CDS             complement(3395..>3985)
                     /locus_tag="VFF05_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW52070.1"
                     /translation="LISALTRWSIHSTLRHASTLPHAPDFVLAINLSPQALSRGGVVE
                     QLMDALNIWGMAPATIVAEVTETALARDMDLAVHALRRLRDHGMRVAIDDFGVGYASI
                     TYLHKFPATELKIDRSLVASIASDAQVARLVESIIQFAHNLGLATTAEGIEDAATQRL
                     LTEMGCDLGQGYYLGRPEPAADFIARFRADSVIATP"
BASE COUNT          794 a         1236 c         1280 g          675 t
ORIGIN      
        1 gagcgcgtgc gccactggtt gcatctgcca gtcgacgccg gacgtttgcg cgcacgcctg
       61 ttcgcgcaag gcctggtggc aaacgaacgc cacgtcgaga gcggctggga aatcgagatc
      121 gacgcgccgc gtgcgctgct cgaacccttg ttcggtctgc ccgcaggcga gggcgagtgg
      181 ctgcgaacac aacttgccgc tgcggatgcg gcttcctaca atccttcgac agcgactgtt
      241 tgagacaacc atggcctgga acgaacccgg aagcggcggc ggcaaacgcg acccttggca
      301 aggcaagcct cctgatttgg acgcgtggct caagcgtctg cgcgacggct tcaaccgctt
      361 gtttcgcggc ggcggcggtg gcggtggaat cggcggcggc tcgggcggca tcgccctgat
      421 cgtgctggcg ctggtcgtcg gctggctggc gctggattcg tggacggtga tcgatgcgcg
      481 ccagttcggc gtggtcctgc gcttcggtca gttcaaccgc gtcatgacca gcggcctgaa
      541 cctgaaatgg ccggcgccgg tggaacgtgt catccgcgtg gatgcgacca acgtgcgttc
      601 ggtgtccgat gaagtgcgca tgctgaccaa ggacgagaac atcgtgctgg tggatttcaa
      661 cgtgcagtac caggtcaccg acgccgaaaa atacctgttc gccgccaatc agccggacga
      721 aaccctcaag caggcggccg aaagcgcggt gcgtcaggtg atcgggcgca gcgcgatgga
      781 cacgatcctt tccggtcacg gttccgaagt cgttggcgaa accaagaaac tgctgcagca
      841 gaccctggac ggttacggcg tcggcctgtt cgtcaccgag atcaatttcc agaaaatcct
      901 gccgccgccg gaagtgaaag aagcattcga cgacgccaac aacgccggca acaaccagca
      961 gcagttcatc aacgaggcgc aaggctacgc ggcgaaagtg gtgccgctgg cgcgcggcga
     1021 ggcagcgcgc atccgtgccg ccgccgaagg ctacaaggcc gcgcagatcg cggtcgcgac
     1081 cggcgataca cagcgttttt cgctgctcga cacgcagtac aaggccgcgc cggaagtcac
     1141 gcgcaagcgt ctgtatctgg agacgctgca aggcgtgctt gccaacaata ccaagatcat
     1201 cgacaccggc aacggcaaga atctgattta tctgccgctg gacaaactca agtccggact
     1261 cgatgcgaca actgccgccg ccgcggcatc cgtggcggac gatacgaaga aaacgggcaa
     1321 ggaggccaat caatgaaact ccttcctccc atcatcgcct tgttgctggt tttgctgatc
     1381 gccaacagcg cgttcatcgt gccggcagga caaagcgccc tggtgctgca attcggccgc
     1441 atcgaaggca gcgcaagcac tgccgccgat tacaagccgg gcttgcattt caagctgccg
     1501 ctggtccagc aagtggtgcg ctacgaccgc cgcatcctca atctcgaagc gcagccggaa
     1561 cgctacttca cgtccgagaa gaaggcggtc aacgtcgatt tctacgtgaa gtggcgcatc
     1621 tccgatccag cggcctatta ccgttcgttc ggcgcggacg agacgcagac gctggccaac
     1681 cagcgcctcg cgccgatcat caaggacgca ctgcgtttcg agttcaccgc gcgcacgctc
     1741 aacgacctga tcgcgaacgc gcgctcggac atcaccgaaa acgtgcgcaa acaggccaac
     1801 gcggcaaccg aaggcaagct cggcatccgc gtcgtcgacg tgcgcatcaa gcgcatcgaa
     1861 ttcccggacg aagtgctcgg ctcggtttac aaacgcatga gcgccgagcg caaccggctg
     1921 gccaacgaac agcgttccaa cggtcaggaa gaggcggcca agatcgaggc cgatgccgat
     1981 cgccaggtgc aggtgatcaa ggccgaggcc gagcgcgacg cgcaggaagc acgcggcgag
     2041 ggcgatgcca aggccgcgga aatctacgcc gcggcatatg ccaaggatcc ggatttctat
     2101 gccttcgacc gcagcctgaa ggcttaccgc gaggcgttca agggtggcag cgtgatcgtg
     2161 gtcgatccga aatccgagtt catgcgctat ttcgacgcga gcaagggcga gggcggcaag
     2221 tagcggaaca tgccgcatgc attcgccgcc gccctgtgcc tggtgctggt gcttgaaggc
     2281 ctgttcctgt tcgccgcgcc acgcgcgtgg cagcaaatgg ccgagcagat gcgacagctc
     2341 gatccgcgcc agttgcgcgt gatcggcggg atcatgatcg cggcgggctt gctgttgctg
     2401 aagctggttg tctgaaccgc gcctgtggcg agcgccgagt cggcgccccg cggtgcggct
     2461 tttcgggtat tattgcgcgc cttcacggcg gcccatcccg cgctgccgcg atgtttccct
     2521 tccggaggtt ttcatgattt tgcgtggcat cttgatagcg gcagcgacgc tggccgcggg
     2581 cgcggcattc gcgcaaaacg gcggttcgac ggcttcggcc agccaggcgc aggctcaaca
     2641 gcaaacgccg cccgcgccgc tcaccgcggc ggacaagccg aacctgtcat acgcgatcgg
     2701 ttaccagatc ggttcggatt tcgtcgaacg caagatcgac atcgacctca acgcagtcat
     2761 ccgcgccatc caggacggtt acgccaagca cgcgccgacg gtcagcgaag acaagatgca
     2821 cgacctgctt gcgcgcatgc agtaccagat gtacaccaag gccaagggcg agttcgacaa
     2881 gctcgctggc gagaacaagg ccaagagcga caaattcctc gccgacaaca agtccaagac
     2941 cggcattcac gtgctgccgt cgggcgtgca gtaccgcgtt atcgaggaag gtaccggcac
     3001 ccagcatccg accctcaaca gcgaagtcac cgtgcattac cgcggttcac tgatgaacgg
     3061 actggaattc gacagctctt tcgcgcgcgg cgagccggtg cacttcaagg tcggcgacgt
     3121 gatcaagggc tggcaggacg tgctgccgct gatgcgcgtg ggcgaccatt ggcagatctt
     3181 cgtgccggcg gctcttgcct acggcgaacg cggccagccg ccacgcatcg gcccgaacga
     3241 ggcgctggtg ttcgagctga agctgatttc gatcaagtaa gttcaggatg aaattatccc
     3301 gtgcagcaca tggatgtgcg atgacacggg atccagttcc aagacaaaaa cgccgcgcgc
     3361 tcgtcgcggc gttttcgttt ttgcgtggat cgtttcaggg cgtggcgatc acggagtctg
     3421 cccggaatcg cgcgatgaaa tcggcggccg gctcgggccg gcccaggtaa tagccctggc
     3481 cgaggtcgca acccatttcc gtcagcaggc gctgcgtggc ggcgtcctcg atgccttcgg
     3541 ccgtggtcgc caggccgagg ttgtgcgcga actggatgat ggattcgacc aatcgcgcta
     3601 cttgcgcatc gctcgcaatc gacgcgacca acgagcggtc gatcttcaat tcggtggcgg
     3661 ggaatttgtg caggtaggta atcgaggcgt agccgacgcc gaaatcgtcg atcgccacgc
     3721 gcatgccgtg atcgcgcagc cggcgcagcg cgtggacggc caggtccatg tcccgcgcca
     3781 gggcggtttc ggtgacctcg gcgacgatcg tggccggcgc catgccccag atattcagcg
     3841 catccatgag ttgttccacg acgccgccac gcgaaagcgc ctgcggcgat agattgatcg
     3901 ccagcacgaa atccggagca tgcggcaacg tggaagcgtg cctgagcgtg gaatggatgc
     3961 tccagcgcgt cagcgcggat atcag
//
LOCUS       DASRVO010000761         1020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_177060, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000761 DASRVO010000000
VERSION     DASRVO010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177060"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFF05_09425"
     CDS             complement(<1..311)
                     /locus_tag="VFF05_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52071.1"
                     /translation="MRDDVRTGALSLTEFAADLHDVMMQRGTRPIYEDPARFFALTYP
                     TFALRELAKDVVLRLAGKNTKAIRELDLTYGGGKTHTLITLRHLVNDTGSLPLIPSVS
                     Q"
     gene            complement(371..688)
                     /locus_tag="VFF05_09430"
     CDS             complement(371..688)
                     /locus_tag="VFF05_09430"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HZW52072.1"
                     /translation="MTAPRIVTTELTPPARSQAPWRVAEVQVLPGFRLRVRCNDGAAG
                     IIAMAEFIASDAAGVFTTLRDEKLFRRARIALGAVTWPGELDLAPDAMHREIKEHGKW
                     IVT"
     gene            complement(685..>1020)
                     /locus_tag="VFF05_09435"
     CDS             complement(685..>1020)
                     /locus_tag="VFF05_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52073.1"
                     /translation="STWRPRLALLYVELDRLADARGEFETLVADNLDELPRDGRWTIC
                     LACLAEIAVALGDAARAETLYKELPRYAGRVLVLGGGLVCCGSSGRHLGLLRRHHGAA
                     PGRNGSLKL"
BASE COUNT          186 a          344 c          320 g          170 t
ORIGIN      
        1 aattgagaaa ccgatggaat aagcggcaga gaccccgtgt cgttcaccaa gtgacgcaac
       61 gtgatcagcg tgtgcgtctt gccaccgcca taggtcaaat ccagctcgcg gatcgccttg
      121 gtgttctttc cggcgaggcg caacacaaca tctttagcca gctcgcgcag cgcgaacgtc
      181 ggataggtca gggcgaaaaa gcgcgccggg tcttcgtaaa tcggccttgt gccgcgctgc
      241 atcatcacgt cgtgcagatc ggcggcgaac tccgtcagtg acagggcgcc cgtgcgcacg
      301 tcgtcgcgca acgtcaccac ctggtgccag ggtttccagg ccaaggtgat tggcttaccg
      361 tttcccgatt tcatgtgacg atccacttac cgtgttcttt gatctcgcga tgcatcgcgt
      421 ccggcgccaa atccagctcg cccggccaag tgacggcgcc caatgcgatc cgggctcggc
      481 ggaacagctt ttcgtcgcgc agcgtggtga acacgccggc cgcatcggag gcgatgaact
      541 ccgccatcgc aatgatcccg gcggcgccgt cgttgcagcg cacgcgcagc cggaagccgg
      601 gcagtacctg cacctccgcg acgcgccacg gcgcctgcga ccgggccggc ggcgtcagct
      661 cagtggtgac aatccttggc gcggtcataa tttcagactg ccattgcggc ctggcgcggc
      721 gccgtgatgc cgccggagca gcccgagatg gcggccgctc gatccgcaac acaccaggcc
      781 gccacccaac acaagcacgc ggccggcata gcgcggcagc tccttgtaca acgtctcggc
      841 gcgggcggcg tcgccgagcg ccaccgcgat ctcggccagg caggcgaggc agatcgtcca
      901 tctgccgtcg cgcggtaact cgtcgagatt gtcagcaacc agcgtttcga actcgccacg
      961 cgcgtcggcg aggcggtcga gttcgacata aagcagcgcc agcctgggcc gccaggtact
//
LOCUS       DASRVO010000762         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_74620, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000762 DASRVO010000000
VERSION     DASRVO010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74620"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFF05_09440"
     CDS             complement(<1..181)
                     /locus_tag="VFF05_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016849077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52074.1"
                     /translation="MVEYAAPFSAPSTMSVPAAKIDLDYTLEHKYTRERGRVYLSGVQ
                     ALVRLPLMQQMRDRAA"
     gene            263..1678
                     /locus_tag="VFF05_09445"
     CDS             263..1678
                     /locus_tag="VFF05_09445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZW52075.1"
                     /translation="MVFNSLTFAAFFALVLVVHNLPLPWTWRKVNLLLASYLFYSAWN
                     PPFVILLWISTVVDWYAAQGLVKARRESARHAWMLLSVIANLGMLGYFKYGTFLLDNF
                     AALLHAAGIAYQPVPSDIVLPVGISFYTFATLSYTLDVYLRRALPAENFLDYALFVTF
                     FPHLVAGPIMRPTELVPQFEQPRRASADQLRFGLALMTLGLFNKVVLADSFLAPVAEH
                     VYDAQAIPGALDAWAATLAFSGQIFCDFAGYSTTAIGAALCLGFAMPDNFRFPYAAVG
                     FSDFWRRWHITLSSWLRDYLYIPLGGNRHGPHRTYFALMGTMLLGGLWHGANWTFVVW
                     GGLHGAYLAAERVLRSRFGHYRPGPLTLLALGLLTWVLVNVTWVFFRAKTFDKAWIVL
                     RGMAGTNVDTKPILESFYLISVAVIVGGILLAHWFMRARTLEGVVARAHPAAISAVWA
                     LLAFAIVAAQGSGAAFIYFQF"
     gene            1681..>2677
                     /locus_tag="VFF05_09450"
     CDS             1681..>2677
                     /locus_tag="VFF05_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52076.1"
                     /translation="MDMRRHDVSPEDQVRYLRRGDYARVTAADRPGVAQPVPERDVPA
                     QPWGRIMLGAAILFALLLGTWEWHWRAFDVRPAYRNDSGLWAIQRRRIDAGEGNATVV
                     IGSSRSFFDLQLDVWQKLDGERPIQLAFEGTTPLPFLEDLAADPNFTGRLIVGVAPQL
                     FFSGFGFHKDAIAFYHKESPSQRVGQCLSMHLIEPFWAFDDDDYALATVLQRQPWWPE
                     RPGKPNSLHVRRLSESGPDRATHLWSKVADDPQYQALARSIWSQHLKPSPDDPPPDKV
                     AKTRDEQIDRVVKAVATLRARGVKVLFLRPPSAGPYLAYDDRLFPRAQAWDVLLAK"
BASE COUNT          398 a          904 c          833 g          542 t
ORIGIN      
        1 cggccgcgcg atcgcgcatc tgctgcatca gcggcaggcg taccagcgct tgcacgccgg
       61 acaggtacac gcggccgcgt tcacgcgtgt acttgtgctc gagggtgtag tcgaggtcga
      121 tcttggcggc gggaacggac atggtggaag gcgcactaaa cggggccgcg tattctacca
      181 gtcgcttgcc cgccgaccgt ggcggcccgg gccggaacct gtatgatcca cgccgttgac
      241 ctttctcggg ggaggccggg aaatggtttt caactcgctc acattcgcgg cgttcttcgc
      301 gctcgtgctg gttgtgcaca acctgccgct gccatggacg tggcgcaagg tcaacttgct
      361 gctcgcgagt tacctgttct attcggcgtg gaatccgccg ttcgtgatcc tgctgtggat
      421 ctcgaccgtg gtcgactggt acgccgcgca aggcctggtc aaggcgcggc gcgagtccgc
      481 acggcatgcc tggatgttgc tgtcggtaat cgccaacctc ggcatgctcg ggtacttcaa
      541 gtacggcacg ttcctgctcg acaacttcgc cgccctgctg catgcggccg gtatcgccta
      601 ccagcccgtg ccaagcgaca tcgtgctgcc ggtcggaatc tccttttata cttttgccac
      661 cttgtcgtac acgctcgatg tgtacctgcg ccgcgccctg ccggcggaga atttcctcga
      721 ctacgcgctg ttcgtcacgt tcttcccgca cctcgtcgcc gggccgatca tgcgcccgac
      781 cgaactggtg ccgcagtttg aacagccgcg tcgcgccagt gccgaccagt tgcgcttcgg
      841 cctcgctctc atgacattgg gcctgttcaa caaggtcgtg ctggccgaca gtttcctcgc
      901 gccggtggcc gagcacgtct acgatgcgca ggcgattccc ggcgcgctcg acgcctgggc
      961 ggcaacactc gccttcagcg gccagatctt ctgcgacttc gccggctatt cgaccacggc
     1021 gatcggcgcc gcgctgtgcc tgggtttcgc gatgcctgac aacttccgct ttccctacgc
     1081 agcggtcggc ttctcggatt tctggcggcg ctggcacatc accttgtcgt cgtggctgcg
     1141 cgattacctg tacattccgc tgggcggcaa ccgccacggg ccgcatcgca cctatttcgc
     1201 gttgatgggc accatgttgc tcggcggttt gtggcacggc gcgaactgga ccttcgtcgt
     1261 ctggggtgga ttgcacggtg cgtacctggc agcggaacgc gtgctgcgtt cgcgtttcgg
     1321 gcattaccgg ccaggaccac tcaccctcct cgcgctcggc ctgctgacct gggtactggt
     1381 gaacgtcacc tgggtattct tccgcgccaa gaccttcgac aaggcctgga tcgtgttgcg
     1441 cggcatggcc ggcacgaatg tggatacgaa gccgatcctg gaatcgtttt atctgatcag
     1501 cgttgccgtg atcgtcggcg gcatcctgct cgcgcactgg ttcatgcgcg cgcgcacgct
     1561 cgaaggcgtg gtcgcgcgcg cgcatccggc tgcgatcagt gcggtttggg cgctgctcgc
     1621 cttcgccatc gtcgctgcgc aaggttctgg cgcggccttc atctatttcc agttctgacc
     1681 atggacatgc gccgccacga tgtgtcgccc gaagaccagg tccgctacct gcgccgcggc
     1741 gattatgcgc gcgtcaccgc cgctgaccgt cccggcgtgg cgcagccggt gccggagcgg
     1801 gacgtgccgg cgcagccgtg gggccgaatc atgctcggcg ccgcaatcct gttcgcgctg
     1861 ctgctcggta cctgggaatg gcactggcgc gcgttcgacg tgcgccccgc ctatcgcaat
     1921 gacagcggcc tgtgggcgat ccagcgccgc cgcatcgacg cgggcgaagg caatgccacc
     1981 gtggtgatcg gctcctcgcg ctcgttcttc gacctgcagc tcgacgtttg gcagaagctc
     2041 gacggcgaac gcccgatcca actcgccttc gaaggcacca caccgctgcc gtttcttgaa
     2101 gatctcgccg ccgatccgaa tttcaccggc cgcctgatcg tcggcgtggc gccgcaattg
     2161 ttcttcagcg gtttcggatt ccacaaggac gcgatcgcgt tctatcacaa ggaatcgccg
     2221 tcgcagcgcg tcgggcaatg cctgtcgatg cacctgatcg aaccgttctg ggccttcgac
     2281 gacgacgatt acgcgctcgc cacggtgctc caacgccagc cctggtggcc ggaacgtccg
     2341 ggcaaaccca actccttgca tgttcgacgt ctgagcgaaa gcggcccgga tcgcgccacg
     2401 catctgtgga gcaaggtcgc cgacgatccg cagtatcagg cgctggcacg ctccatctgg
     2461 agccagcatc tcaaaccctc gcccgacgat ccgccgccgg ataaagtcgc caagacgcgc
     2521 gacgaacaga tcgaccgcgt cgtgaaagcg gtcgcgacct tgcgcgcacg cggcgtcaag
     2581 gtgctgttcc tgcgcccgcc gagcgcaggt ccctatctgg cttacgacga ccgcctgttc
     2641 ccacgcgcgc aggcctggga tgtgctgctc gcgaaat
//
LOCUS       DASRVO010000763         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_386089, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000763 DASRVO010000000
VERSION     DASRVO010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386089"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2168
                     /locus_tag="VFF05_09455"
     CDS             <1..2168
                     /locus_tag="VFF05_09455"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HZW52077.1"
                     /translation="LGISALWLDAREHLAAAPLPNQNAWGRYLSASVPTAPDAAFALA
                     LGKRGALFVTQGFTARNPQGETVTLGRGGSDTSAGYFGALLKAERVEIWTDVAGMFSA
                     NPRQVPNARLLARLDYEEAQEIATTGAKVLHPRCINPLREVRVPLAIKDTNFPDLAGT
                     QIGAVAPDAAPSVKAISSRGGITLIAMESIGMWQQVGFLADVFEHFKRHGLSVDLVGT
                     SETNITVSLDPTENLVNSDVLSALCADLAKVCRVKVIAPCAAITLVGRGMRSMLHKLS
                     GVLAEFGPQRVHLISQSSNNLNLTFVVDENIAADMVPALHALLIRAEAMRVEDRSIFG
                     PSWRDLYKTGQSAQAAPWWRVRRAELLELAQAHSPRYVYDPAVVRERAQALRRDVACV
                     DRWFYAIKANAHPALLAEIVAQGFGLECVSPGELERARQVSGAALLFTPNFAPRAEYA
                     DALARKIPLTLDNLHPLAQWGADFAGREIFLRLDLGSGRGHHDKVKTGGAKSKFGLAL
                     TELPEFQRLAARHDVRIVGLHAHLGSGILDAAHWRDVYVQLASIGEKIGSVHVLNIGG
                     GLGVPSRADESPLDLAALGTALAEVKTAYPQFELWAEPGRYLVADAGVLLARVTQTKH
                     KGEVHYIGCDAGMNSLIRPALYEAYHEIVNLTRLDEPATELVQIVGPICESGDVLGSN
                     RRLPPTREGDVLLIAQTGAYGAAMASHYNLRQPAAEVVL"
     gene            2171..>3007
                     /locus_tag="VFF05_09460"
     CDS             2171..>3007
                     /locus_tag="VFF05_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014404103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HZW52078.1"
                     /translation="MHDPRQAKTFRFVRCAYANGEAQLVYAFDDGPELIERIGFPDAP
                     ALLPEREAAFSAALRLLHLIAGVSYYKAGVPGQIVVEGEALDAATAQFMDQVYLHGLA
                     EFAYQNKLDLCGRILFPRNGVASDAAPSLHLGRRTLVPIGGGKDSLVSVEMLKRLGEP
                     AAAVWVGESALIAACAQRTDLAMLNLKRRLAPELFEYNRLGAYNGHIPVTAINSAILV
                     CAAILYGFDAIAFSNERSASEATLEYDGQPVNHQWSKSLEFERGLREYTTSHIAANLD
                     YFS"
BASE COUNT          519 a          952 c          974 g          562 t
ORIGIN      
        1 ggttgggcat ttcggcacta tggctggatg cgcgcgaaca tcttgccgct gcaccgctgc
       61 cgaaccagaa cgcgtgggga cgctatcttt cggcttcggt gccgaccgcg ccggatgcgg
      121 cgttcgcctt ggccctgggc aaacgcggtg ctcttttcgt gacacaagga ttcacggcac
      181 gcaatccgca gggcgaaacc gtgaccctcg gccgcggcgg gtccgacacg tcggccggct
      241 atttcggcgc gctgctcaag gcagaaagag tggaaatctg gacggatgtc gccggcatgt
      301 tcagcgccaa tccgcgccag gtgccgaacg cgcgcctgct cgcgcggctc gattacgaag
      361 aggcgcagga aatcgccacc accggcgcca aggtactgca tccgcgctgc atcaatccgt
      421 tgcgcgaggt gcgcgtgccg ctggcgatca aggacacgaa ttttccggat ctcgccggta
      481 cgcaaatcgg cgcggtcgcg cccgacgccg cacccagcgt caaggcgatc agctcgcgcg
      541 gcggcattac cctgatcgcg atggaatcca tcggcatgtg gcagcaggtc ggttttctcg
      601 ccgacgtgtt cgaacacttc aagcgccacg gcctgtcggt ggacctggtc ggcacctcgg
      661 agacgaacat caccgtgtcg ctcgatccga ccgagaacct ggtgaattcg gatgtgctat
      721 cggcgctgtg cgcggatttg gcgaaagtct gccgcgtgaa agtcatcgcg ccgtgcgcgg
      781 cgatcacact ggtcgggcgc ggcatgcgct cgatgctgca caagctctcc ggcgtgctcg
      841 cggaattcgg cccgcagcgc gtgcatctga tttcgcaatc gtcgaacaat ctcaatctca
      901 ccttcgtcgt cgacgagaac atcgccgcgg acatggtgcc ggcgctgcat gcattgctga
      961 tccgtgccga ggcgatgcgc gtggaggatc gatcgatctt cgggccgagc tggcgggatt
     1021 tgtataaaac cggacaatcc gcgcaagccg caccgtggtg gcgggtgcgt cgcgccgaat
     1081 tgctggaact tgcgcaggcg cattcgccgc gttacgtcta cgatcccgcc gtcgtgcgcg
     1141 aacgcgcgca ggctctacgc cgcgatgtcg cctgcgtgga tcgctggttc tacgcgatca
     1201 aggcgaatgc gcatccggcc ttgctcgccg aaatcgtggc gcagggcttc ggcctggaat
     1261 gcgtgtcgcc gggcgagctg gaacgtgcca ggcaggtgtc aggtgcggcg cttctgttca
     1321 cacccaactt tgcgccgcgt gccgaatatg ccgacgcgct cgctcgcaag attccgctta
     1381 cgctcgacaa cctgcatccg ttggcgcaat ggggcgcgga ttttgccggc cgcgagattt
     1441 tcctgcgcct cgatctcggc agtggccgtg gccaccacga caaggtcaag accggcggtg
     1501 ccaaatccaa attcggcctc gcgttgaccg agctgccgga attccagcgt cttgccgcac
     1561 gtcacgacgt gcgtatcgtc ggactgcacg cgcacctggg ttcgggaatc ctcgacgccg
     1621 cgcactggcg cgacgtctac gtgcagcttg ccagcatcgg cgagaaaatc ggcagcgtgc
     1681 atgtgctcaa catcggcggt gggctcggcg tgccgtcgcg agccgacgag tcgccgctcg
     1741 atctcgccgc gctcggcacg gcgctggccg aagtcaagac ggcgtatccg caatttgaat
     1801 tgtgggcgga accgggccgc tatctcgtcg ccgacgctgg cgtcctgctt gcccgcgtca
     1861 cgcaaaccaa gcacaagggc gaagtgcatt acatcggctg cgacgccggc atgaattcgc
     1921 tgattcgacc ggccttgtac gaggcgtatc acgagatcgt gaacctcacg cgcctcgacg
     1981 agccggcaac ggaactcgtg cagatcgtcg gcccgatctg cgaatccggc gacgtgctcg
     2041 gcagcaaccg ccgcctgccg ccgacgcgcg aaggcgacgt gttgctgatt gcgcagaccg
     2101 gggcctacgg cgcggcgatg gcttcgcatt acaacctgcg acaaccggcg gcggaggtgg
     2161 tcctgtgacg ttgcatgacc cacgccaagc caagaccttc cgtttcgtgc gctgtgccta
     2221 tgcgaatggt gaggcacaac tcgtctacgc gttcgacgat ggcccggaac tgatcgagcg
     2281 catcgggttt ccggatgcac cggcattgct gccggagcgt gaagctgcat tctccgccgc
     2341 gctgcgcctg ctccacctga ttgcgggcgt cagctactac aaggcgggcg tgcccgggca
     2401 aatcgtcgtc gaaggtgagg cgctggatgc agcgacggcg cagttcatgg atcaggtcta
     2461 tctgcacggc ctggccgaat tcgcttatca gaacaagctg gatttatgcg ggcggattct
     2521 ttttccgaga aatggcgttg cttcggatgc tgcaccgtcg ttgcatctcg ggcgccgcac
     2581 ccttgttccc atcggcggcg gcaaggattc gctggtcagc gtcgaaatgt tgaagcgcct
     2641 cggcgagccg gcggcagcgg tgtgggtcgg cgagtcggcg ctgatcgccg cctgcgcgca
     2701 gcgcacggat ttggcaatgc tgaatctcaa gcgcaggctc gcgccggaac tgttcgagta
     2761 caaccgcctg ggcgcgtaca acgggcatat tccggtcacc gcgatcaatt cggcgatcct
     2821 ggtctgcgcg gcgatcctgt atggcttcga tgcgatcgcg ttctccaacg aacgctcggc
     2881 ttcggaagcg acactcgaat acgacggcca gccggtcaat caccaatgga gcaagtcgct
     2941 ggaattcgag cgcggcctgc gcgagtacac gacctcgcat atcgcggcga acctggatta
     3001 tttctcg
//
LOCUS       DASRVO010000764         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_99850, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000764 DASRVO010000000
VERSION     DASRVO010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99850"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VFF05_09465"
     CDS             <1..1034
                     /locus_tag="VFF05_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52079.1"
                     /translation="SYALELLSGIAARRHDSSRALEYAQRTVESGADEVRFNPADLGA
                     WTRWTLAYRGLAESQFDLGEVTQAIATMHALLALEQDPRRPSSLGPIVWYNWLQLAAW
                     QEQAGDAAGAQLSTNGLEHDVAEVVAQFAQDDPRYQLLAAPGDSIRGRLRLSAGDPQG
                     ALTAVPAYIARIEAITVPASNTAATFMKRNILDGNLGVAAAAAIRTGHYAQAEGFARR
                     KLALPIDRTTEGDPETSHNEARALLAHAIALQGRLDEARSTLQPALDHYRQQQVAGAK
                     ETYFRRAFAYALYVDAVAQPADAAGGKQRAADLDEAAKQIAGASAEAQKLTDMRYVSD
                     LIAAAARGG"
     gene            1031..2737
                     /locus_tag="VFF05_09470"
     CDS             1031..2737
                     /locus_tag="VFF05_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52080.1"
                     /translation="MTARAAIPLTIGVTSHRNLVASEVEAIRQGVRDFLARMRREHPH
                     MPLVVVSALAEGGDQLVAEEALAAGARLIAPLPLARAQYAEDFTDLAARGRFNALCDA
                     AQVIELPQLTNGAVHAPVPTGHERDRHYAQSGVYVSDHCHILLAIWDGKPSTLLGGTA
                     QIVRYRLSGEKPALAERRRAAPRNLLDDDSERLAYHIVCSRDQADGAPSPSLHPLQTV
                     WRCGEQIQPGDGSMPEKFRATLANAAEFNADAEKYAAPIDAHPGLSAVATDAGAIERL
                     FRAADWLAIHFQRRVLLAMRGLYTVAALMAIAFTAYDNLPAQDDMLYVFLLLFVLGGF
                     LVVLANRRGWHRKYLDYRAFAEGLRVQWYWRRIGLSLAGDAEFARDNFLQKQDVELGW
                     VRDAMRSAELESAPRNVADATREMGDVVREWVGDTQHGGQLDYYERKAAQRARTHRIT
                     ETVDSASLCVGIVISIVLAAFAVHLSADTKNDLVVVMAIFSIVAGVRAAYAYKKADKE
                     LIKQYRYMQRIFGAARKALDRAVDAAEQREVLRLLGEAALAEQVEWALMHRQRPLEHN
                     RL"
     gene            complement(2758..2928)
                     /locus_tag="VFF05_09475"
     CDS             complement(2758..2928)
                     /locus_tag="VFF05_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52081.1"
                     /translation="MPKPNYKYDKRQRDLAKKKKQDEKRLRKLEKGRTADPAPAAPSD
                     DSAGEVTQQIES"
     gene            complement(3022..3231)
                     /locus_tag="VFF05_09480"
     CDS             complement(3022..3231)
                     /locus_tag="VFF05_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZW52082.1"
                     /translation="MAERQIGTVKWFNDAKGFGFITPESGPDLFVHFRSIQGAGFKTL
                     AEGQRVSFVAVQGQKGMQADEVQLA"
     gene            complement(3466..>3831)
                     /locus_tag="VFF05_09485"
     CDS             complement(3466..>3831)
                     /locus_tag="VFF05_09485"
                     /inference="COORDINATES: protein motif:HMM:NF019883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZW52083.1"
                     /translation="LQPPWLFATLRQPGIDLLAELVTLCRERPAMSTAVLLEHYEGRE
                     EARALRKLATLDFPGEDDALRAEFVAAIGQLDRQTHQQRLDDLLKKQGEAALDADEKD
                     ELRRLLAAKNAPRPRESQN"
BASE COUNT          652 a         1224 c         1264 g          691 t
ORIGIN      
        1 gctcctacgc cctggaactg ctaagtggca tcgctgcgcg ccggcatgac agctcgcgtg
       61 ctctcgagta tgctcagcgc acggtcgagt cgggcgccga cgaagtccgg ttcaaccccg
      121 ccgacctcgg tgcgtggacg cgttggacac tggcgtaccg cggtctcgcc gagagccagt
      181 tcgatctcgg cgaggtgacg caggcgatcg ccacgatgca cgccttgctg gctctggaac
      241 aggatccacg ccggccttcg agcctgggtc cgatcgtctg gtacaactgg ttgcagctgg
      301 cggcctggca agagcaagct ggcgatgcgg cgggtgccca actgtctacg aatggcctcg
      361 aacacgatgt cgcggaagtg gtcgctcaat tcgcgcagga cgatccccgg taccaattgc
      421 tggctgcgcc gggcgactcg atccgtggcc ggctgcgact gagcgccggc gatccgcaag
      481 gcgcgctgac cgctgttcca gcctatatcg cgcgcatcga agccattacg gtgccggcct
      541 ctaacactgc agccacgttc atgaagcgca acatcctcga cgggaatttg ggtgtcgccg
      601 ccgcggccgc gatccgcacc ggtcattacg cacaggccga gggcttcgcg cgccggaaac
      661 tggcgctgcc gatcgaccgc accacggaag gcgatccgga aaccagtcac aacgaggcgc
      721 gtgcgctgct cgcccacgcg atcgccctgc aggggcgcct cgacgaggcg cgttcgacac
      781 tgcaaccggc gctggaccac tatcgccagc agcaggtggc gggcgccaaa gagacatatt
      841 tccgcagggc gtttgcctac gcgctttacg tcgacgctgt cgcgcaaccg gccgatgccg
      901 caggcggcaa gcagcgtgct gccgatctgg atgaagcggc gaagcagatc gctggcgcct
      961 cggcggaagc gcagaagttg acggacatgc gctatgtgtc cgacctgatc gcggcagctg
     1021 cgcgcggcgg atgactgcac gcgccgccat accgctgacg atcggcgtca ccagtcatcg
     1081 caacctcgtc gccagcgagg tcgaggcgat ccgacaaggc gtacgcgatt tcctcgcgcg
     1141 catgcggcgc gagcatccgc acatgccgct cgtcgtcgtg tccgcgctgg ccgaaggcgg
     1201 cgatcaactc gtcgcggaag aagcgctcgc cgccggcgcg cgcctgatcg cgccgctgcc
     1261 actggcgcgc gcgcagtacg ccgaggattt caccgatttg gccgcgcgcg ggcgcttcaa
     1321 tgcgctctgc gatgcggcac aagtcatcga gctgccgcag ctcacgaatg gcgccgtcca
     1381 cgctcccgtt ccgaccgggc acgagcgcga ccggcattac gcgcaatccg gcgtctacgt
     1441 cagcgatcat tgccacattc tgctggcgat ctgggacggg aagccatcga ctctgcttgg
     1501 cgggacggcg cagatcgtgc gctatcgcct gagcggcgaa aagcccgcgc tggccgagcg
     1561 ccgtcgcgcc gcgccgcgca acctgctgga cgacgacagc gagcgccttg cgtatcacat
     1621 cgtgtgttcg cgcgatcagg ctgatggcgc gccgtcgccg tcactgcatc ccttgcagac
     1681 cgtttggcgc tgcggcgagc aaatccagcc tggcgatggt tcgatgcccg aaaaattccg
     1741 cgcgacgctg gccaatgcgg ccgagttcaa tgcggatgcg gaaaaatatg ccgcgccgat
     1801 cgatgcgcat ccgggattgt cggccgtcgc gacggacgcc ggcgccatcg agcggctgtt
     1861 ccgcgcggcg gactggctgg cgatccactt tcagcgtcgc gtgctgttgg ccatgcgcgg
     1921 gctctatacc gtggccgcgt tgatggcgat cgcgttcacg gcttacgaca acctgcccgc
     1981 gcaggacgac atgctctacg ttttcctgct gctgttcgtg ctcggcggtt tcctcgtcgt
     2041 cctcgccaac cgccgcggct ggcatcgcaa atacctggat taccgtgcgt ttgccgaggg
     2101 tttgcgcgtg caatggtact ggcggaggat cgggctgtcg ctcgctggcg acgctgaatt
     2161 cgcgcgtgac aatttcctgc agaaacaaga tgtcgaactc ggctgggtgc gcgacgcgat
     2221 gcgcagcgcc gaactggaaa gcgcgccgcg caacgttgcg gatgcgacac gcgaaatggg
     2281 ggatgtcgtc cgcgaatggg ttggcgacac gcagcatggc gggcagctcg actactacga
     2341 gcgcaaggcc gcacaacgcg cacgcacgca tcgcatcacc gaaacggtcg actcagccag
     2401 cctgtgcgtg ggcatagtca tcagcatcgt gcttgccgcg ttcgcggtac atctgtccgc
     2461 ggatacgaag aacgacctgg tcgtggtcat ggcgattttt tccatcgtcg ccggcgtgcg
     2521 cgcggcgtac gcctacaaga aggccgacaa ggaactcatc aagcaatacc gttacatgca
     2581 gcgcatattc ggcgcggcgc gcaaggcgct cgatcgcgcc gtcgatgcgg ccgagcagcg
     2641 cgaagtgctg cgcctgctcg gcgaagccgc attggccgag caagtggaat gggcgctgat
     2701 gcaccggcaa aggccgctgg agcacaaccg gctttgagcg ggcgtggtaa gcgccttcta
     2761 gctctcgatc tgctgggtaa cttcgccagc gctgtcgtcg gagggcgcgg cgggcgccgg
     2821 atctgcggta cggccttttt ccagtttgcg caggcgtttt tcgtcctgct tctttttctt
     2881 cgcgagatcg cgctggcgct tgtcgtattt gtagttcggt ttgggcattg ggttttagcg
     2941 tccgcctgtg cgggtgtcga ttgcataaaa aaccccggcc attgctgacc ggggtttttg
     3001 cgtacctatg cggcgcgtgg cttacgccaa ctgcacttca tcggcctgca tgcctttctg
     3061 accctgcacg gcgacgaagc tgacgcgctg gccttcggcg agggtcttga aacccgcgcc
     3121 ctggatggaa cggaaatgca cgaacaggtc cgggccgctt tccggcgtga tgaaaccaaa
     3181 acctttcgca tcgttgaacc acttgacggt accgatctga cgctctgcca taacaaactc
     3241 ctgaaactat gatggatgac gctgcccgga gacattccgg accgcgactg ttttgcaagg
     3301 agaaagcgag gagggcgatg aagctaatcg cgagattgac cgcatcaggc cacgattcac
     3361 cgtgaccccg gcaaatacag cggggcgagt atacagaaga ttggcgcgat acgcgaatcc
     3421 aggccggaag cccccggtgg cccgccgatc atggcgtgcg gggcatcaat tctggctttc
     3481 ccgggggcgc ggggcgtttt tcgcggccag caagcggcgc agttcgtcct tttcgtccgc
     3541 gtccagtgcc gcctcgccct gctttttcag caaatcgtcc aggcgctgct ggtgggtctg
     3601 ccggtcgagc tgcccgatcg cagcgacgaa ttcggcgcgc agggcgtcgt cttcgcccgg
     3661 aaagtccagt gttgcgagct tgcgcagcgc gcgggcttcc tcgcggcctt cgtagtgttc
     3721 gagcagcacg gcggtggaca tcgccggccg ctcgcggcag agcgtgacga gttcggcgag
     3781 caggtcgatg ccgggctggc gcagggtcgc gaacagccac ggcggctgca a
//
LOCUS       DASRVO010000765         1233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_283278, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000765 DASRVO010000000
VERSION     DASRVO010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283278"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFF05_09490"
     CDS             <1..452
                     /locus_tag="VFF05_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52084.1"
                     /translation="RKLVILLRALGHDIELADVETVPLVDGGHLAESDPRPLLARLES
                     LDELWRARVAVAQARRERWIYRASFDGSHARVAPERVSADHALAKLAPCENALILHSR
                     YYRAAPLTIAGPGAGVELTAAAVFADLLDVARRNIAFPNAELPAVAA"
     gene            500..>1233
                     /gene="pgk"
                     /locus_tag="VFF05_09495"
     CDS             500..>1233
                     /gene="pgk"
                     /locus_tag="VFF05_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012511960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZW52085.1"
                     /translation="MSILRMTDLDLAGKRVLIREDLNVPLQDGRITATQRLDAALPTI
                     RHARDAGARVMILSHLGRPKEGVFEESESLKPVADWLSHALGRPVRLIRDYLGGVDVA
                     PGEVVLLENCRMNVGEGKDSPELSAKYAALCDIYVMDAFGTAHRAQASTHGAIRAAKV
                     ACGGPLLMAELDALAKALEHPARPLLAIVAGSKVSTKLALLESLIAKVDQLIVGGGIA
                     NTFIAAAGFNIGKSLVETDLIPTARK"
BASE COUNT          209 a          403 c          408 g          213 t
ORIGIN      
        1 cgcgcaaact cgtcatcctg ctgcgtgcgc ttggccacga catcgaactc gccgatgtgg
       61 aaacagtgcc gctggtcgat ggcgggcatc tggccgaatc cgatccgcgg cctttgcttg
      121 cacggttgga aagcctcgac gaactttggc gcgcacgcgt ggcggtggca caggcgcggc
      181 gcgaacgctg gatttatcgc gcgagtttcg acggatcgca tgcgcgcgtc gcgcccgagc
      241 gggtgagcgc cgatcacgcg ctggccaaac tcgcgccctg cgaaaacgcg ttgatcctgc
      301 acagccgcta ttaccgtgcc gcaccgctga ccatcgccgg gcccggcgct ggcgtggaat
      361 tgacggcggc ggcggtgttc gccgatctgc tcgacgtggc gcgtcgcaat atcgccttcc
      421 cgaatgcgga gctgccagcc gtcgcggcct gagcgcgcgc ctgaattaca atacgcgctc
      481 tcataattgc ggagcgccca tgtccatctt gcgcatgacc gatctggacc tcgccggcaa
      541 gcgcgtgctg atccgcgagg atctgaacgt acccttgcag gacggccgca tcactgccac
      601 gcagcgcctg gacgcggcgt tgccgacgat ccgccacgcg cgcgatgccg gcgcgcgggt
      661 gatgatcctg tcgcacctgg gtcgtccgaa agagggcgtg ttcgaggaat ccgaatcgct
      721 caagcccgtg gccgattggc tgagtcacgc actcggcaga ccggtgcgtc tcattcggga
      781 ttacctgggt ggcgtggatg ttgcgccggg cgaagtcgtg ctgctggaaa actgccgcat
      841 gaacgtgggc gagggcaagg attcgcccga actctcggcc aagtacgccg cgctgtgcga
      901 catctacgtg atggatgcgt tcggcaccgc gcaccgcgcg caggcgtcca cgcatggtgc
      961 gatccgcgcg gcgaaggtcg cctgtggcgg cccgttgctg atggctgaac tcgacgcgct
     1021 ggccaaggcg ctcgaacatc cggcgcggcc gctgctcgcc atcgtcgccg gatcgaaagt
     1081 ctcgaccaag ctagccctgc tcgaaagcct gattgcgaaa gtggaccaac tcatcgtcgg
     1141 tggcggcatc gcgaacacgt tcatcgccgc ggccggattc aacatcggca agtcgctggt
     1201 cgaaacggac ctgatcccca ccgcgcgaaa gat
//
LOCUS       DASRVO010000766         1347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_283303, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000766 DASRVO010000000
VERSION     DASRVO010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283303"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>1347
                     /locus_tag="VFF05_09500"
     CDS             116..>1347
                     /locus_tag="VFF05_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW52086.1"
                     /translation="MTGFATAADDALVQALPMPDTGKLPPDVASQLARSRTDFDNTRV
                     GLVGDDLALAYATIGAVYFRAGLNDVAAVAFNDASRLAPKDARWLYLRGVAASALKRN
                     ADARADFEAALALDKVYLPIRYRLSDTLIEMGNLDAAHAVLAAALQAGAKQAAPFAMI
                     GRLEIRQKNYAAAIDHLQQALKLEPQANALYKDLAEAYAGQGDAAQAKAALAKSGTVP
                     PNLADPLVAGILGASAPTAPALHGTPLAQARQLLARRDFVQARDKTVEALTANPDDVE
                     ALALQARLDGLMGNRAFAQDEAARALKLSPGGASANLSQGMVHEFAGSAAQALPFYQR
                     AVRADADQPDARLLLGNALMRQGDYAQAAEQYRQLVRILGADASAEARFAAAQAMAGQ
                     CRDALDEINRRLVKRAQDG"
BASE COUNT          223 a          473 c          438 g          213 t
ORIGIN      
        1 cgcgaactgg cccgcgccgc cgtgcgtcgt tacactttgc gcccctgttc gtctcgcggc
       61 taatccgatg aagcgtttgc ctatcctgat ttgcatcgcg ctgtgcgcga tttccatgac
      121 cggcttcgcc actgccgccg acgatgcgct cgtacaggcg ttgcccatgc ccgacaccgg
      181 caagttgccg ccggatgttg ccagtcagct cgctcggtcg cgcacggatt tcgacaacac
      241 gcgcgtgggc ctggtcggcg acgacctcgc cctggcctat gccaccatcg gcgcggttta
      301 tttccgcgcc ggcttgaacg acgtcgccgc ggtcgcgttc aacgacgcca gccggctcgc
      361 gcccaaggat gcgcgttggc tgtacctgcg cggtgtggcc gcgagcgcac tcaagcgcaa
      421 cgcggatgcg cgcgccgact tcgaagctgc gctggcactg gacaaggtct atctgccgat
      481 ccgctatcgt ctgtccgaca ccctcatcga gatgggcaac ctggatgccg cgcatgcggt
      541 gctggccgct gccttgcagg ctggcgccaa gcaggcggcg ccgtttgcca tgatcggccg
      601 gctggagatc cgccagaaaa actacgcggc ggcgatcgac catttgcagc aggcactcaa
      661 actcgaaccg caggcgaacg ccctgtacaa ggatctggcc gaggcttacg ccggacaagg
      721 cgacgcggcg caggccaagg cagcgctagc caagtccggc acggtgccgc cgaatctcgc
      781 cgatccgctg gtggcgggaa tccttggcgc ttccgcaccc acggcgccgg ccttgcacgg
      841 cacgccgctg gcacaggcgc ggcaactgct ggcgcgccgc gattttgtgc aagcgcgcga
      901 caaaacggtc gaagcgctca cggcgaatcc ggacgatgtc gaggcgctcg ccctgcaggc
      961 gcgcctggac ggcctgatgg gcaaccgcgc cttcgcccag gacgaagccg cacgcgcgct
     1021 caaattgagt cccggcggtg ccagcgcgaa tctcagccag ggcatggtcc acgaattcgc
     1081 aggttcagcc gcgcaggcat tgccgttcta ccagcgcgcg gtccgcgccg acgccgatca
     1141 acccgatgca cgcctgttgc tgggcaatgc gctgatgcgg caaggcgatt atgcgcaagc
     1201 agccgagcaa taccggcagc tcgtgcgcat cctcggcgcc gatgccagtg ccgaagcgcg
     1261 cttcgccgcc gcacaggcca tggccgggca atgccgcgac gccttggacg agatcaaccg
     1321 gcgcctggtc aagcgcgcgc aggacgg
//
LOCUS       DASRVO010000767         1125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_256145, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000767 DASRVO010000000
VERSION     DASRVO010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256145"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..>1125
                     /locus_tag="VFF05_09505"
     CDS             449..>1125
                     /locus_tag="VFF05_09505"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HZW52087.1"
                     /translation="MRWRIQPRKGRSLDELADGADRVRILTDIFEAPNPDDSFRSFAE
                     QVLEDLIVGGFGSAELRLIRDAERPLQLWPVDGATIKMRSDWDGRPDSPRYVQTTGKM
                     GPNAEIVLKDDELTYMRLNPRTHTPFGLGRLEVAFETINEFLSAHRFAAKLAANSVVQ
                     YALWLQDLTPEHHERLIRWWQDDIEGTGRVPILSVEHKPEVLRFAAGTDADLRLQWQE
                     FLLRIIA"
BASE COUNT          222 a          358 c          353 g          192 t
ORIGIN      
        1 cgcgcgattg gagaatgcga aaacatcgct caccgccgtg gtggaagacg acctcgaccg
       61 cggcgacgac gccatcggct tcgcactgga aacgtttgag aacacgtcta tactcgtggc
      121 caccacgtcc gagctgccgc agattgcgca gagggcgcgg atggagatga gggtgtgacc
      181 gcttcgtgct gaactgacct gacacgcatc agtatttagg aaatccatga acatcactga
      241 agctgttcgc ggcctattgg gccgcaatcg cacacgtgtg tctgccgagg cggacaagcg
      301 acgcaccgtc gcgctaccgt cgatcctcaa ccgctatggt tcgcgtcagg acgcgatgcc
      361 gaagccgacg ccggcgaacc tgcgccgctt tgccgagacg cctgtcgcgc gcaaggccat
      421 caacaccatc aaggatcgca tcgccggaat gcgctggcgc attcagccgc gcaaggggcg
      481 ctcgctcgac gagttggccg acggcgcgga tcgcgtgcgc atcctgacgg acatctttga
      541 ggcgccgaat ccggacgata gcttccgctc gtttgccgag caggtgctgg aagacctgat
      601 cgtcggcggc ttcggctcgg ccgagttgcg cttgatcagg gacgccgagc gtccgctgca
      661 gctgtggccc gtcgatggtg cgaccatcaa gatgcgctcc gactgggatg gccgtccgga
      721 ttcgccccga tacgtgcaga ccacaggcaa gatggggccg aatgccgaga tcgtcctgaa
      781 agatgacgaa ctgacgtaca tgcgcctgaa cccgcgcacc cacacaccgt tcggcctggg
      841 acggctcgaa gtcgcgttcg agacaataaa cgaattcctc agcgcgcacc ggttcgctgc
      901 caagctggcg gcgaactccg tggtgcagta cgcgctctgg ctgcaagatc ttacgcccga
      961 gcaccacgaa cgcctgattc gctggtggca ggacgacatc gaaggcacgg gccgcgtgcc
     1021 gattctttcg gtcgagcata agccagaagt gctgcgcttc gccgccggca ccgatgccga
     1081 cctacgcctg cagtggcagg aattcctgct gcgcatcatc gccga
//
LOCUS       DASRVO010000768         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_151668, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000768 DASRVO010000000
VERSION     DASRVO010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151668"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFF05_09510"
     CDS             <1..399
                     /locus_tag="VFF05_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52088.1"
                     /translation="YRLRPEWHARPMGSLLRRLRVRSPAIRIGRASLLAAALLLGATS
                     VFAAAVYHVAQRHRAFSVGEITIRRGDSIAFDNEDEFIHQIYVDSNLMDVDSREQYPG
                     QIISVQFSKGGTFAVRCHIHPKMLLIVHVK"
     gene            484..>1404
                     /locus_tag="VFF05_09515"
     CDS             484..>1404
                     /locus_tag="VFF05_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZW52089.1"
                     /translation="MRTIVRGFPIVIDHLAVLRRSARRSIGTTIFVGFAAMGVITAAL
                     GGYGLVVLSAAGHFVADTYDVSLMAVSYARSASLDFSRMETALLRRSAAQPGDRSAIT
                     LTLDKMEEGFAADLRVVADRSRTADERRLIAEIRALATRWRQLRDTGSDRDERDAIGR
                     RIIEWLDMLIEITTDHGFIARRIAMTNVARFSYGSIAAIGLALTLSAGITLVLTRRIR
                     RPLAAAAAAADRIAGGQLDTPIPRGGEDETGVLLRSMRVMRDSIRVMVEREAAQRRSA
                     QNRLADALESSREAIVLVDDAGRIVIANSQL"
BASE COUNT          238 a          438 c          464 g          264 t
ORIGIN      
        1 tacaggctgc gcccggaatg gcatgctcgc cctatgggtt ccctgttgcg ccgacttcgg
       61 gtgcgcagcc cggcaattcg gatcggccgc gcctccctgc tggcggcggc gctgctcctt
      121 ggcgccacca gcgtgtttgc cgccgcagtt taccacgtcg cgcaaaggca ccgggccttt
      181 tcggtcggcg agatcacgat caggcgcggc gacagcatcg ccttcgacaa cgaagacgag
      241 ttcatccacc agatttatgt cgactcgaat ctcatggatg tcgattcacg cgagcaatac
      301 ccaggacaaa taatttcggt acaattcagc aaaggaggga cgtttgccgt tcgttgtcac
      361 atccatccaa agatgctgtt gatcgttcat gtgaagtaga ctgtgatctt gcgttaactg
      421 gttgctcata aagttaactg gttgctcata aaaagtggat aaggtcggat aaagccggca
      481 gatgtgcgga cgatcgtgcg cggtttccct atagtcattg atcaccttgc cgttctccgg
      541 aggagcgcgc ggcgttcgat cgggacgacg atcttcgtcg gcttcgcggc gatgggcgtg
      601 atcacggcgg cgctcggcgg gtacggtctg gtggtgctgt cggccgccgg gcactttgtc
      661 gccgacacct acgatgtctc gctgatggcg gtcagctacg cccggtcggc gagcctcgac
      721 ttcagccgca tggagactgc gctgctgcgg cgcagcgcgg cgcagcccgg cgaccggtcg
      781 gcgatcaccc tcacgctcga caaaatggaa gaaggcttcg cggcagacct tcgggtcgtg
      841 gccgatcggt cgcggaccgc ggatgagcgg cgcctcatcg cggaaatccg cgcgctcgcc
      901 acccgttggc gccagctgcg cgacaccggc agcgatcgcg acgagcgcga tgcgatcggg
      961 cgccgcatca tcgaatggct cgacatgctg atcgagatca cgaccgacca cggcttcatc
     1021 gcgcgccgca tcgcgatgac taacgttgcg cggttttcct atggcagcat cgccgcgatc
     1081 gggctggcgt tgacgctgtc ggcggggatc accctggtgc tgacgcgccg tatccggcga
     1141 ccgctggcgg ccgcggcagc cgcggccgac cgcattgccg gcggccagct cgataccccg
     1201 atcccgcgcg gcggggagga cgaaaccggt gtgctgctgc gctcgatgcg ggtcatgcgc
     1261 gacagcatcc gcgtcatggt agagcgcgag gcggcgcagc ggcgctcggc gcagaaccgt
     1321 ttggccgatg ccctggagag ctcgcgcgaa gcgattgtgc tggttgatga tgccgggcgc
     1381 atcgtcatcg ccaacagcca gctc
//
LOCUS       DASRVO010000769         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_386284, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000769 DASRVO010000000
VERSION     DASRVO010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386284"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFF05_09520"
     CDS             complement(<1..556)
                     /locus_tag="VFF05_09520"
                     /inference="COORDINATES: protein motif:HMM:NF006562.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HZW52090.1"
                     /translation="MKLLIVQTGTAIPAVRRQHGDFAQWFRRSIGLSPRQTQCMRVDR
                     GAQLPPPRSVSGVVVTGSGAMVTDRLQWSEATARWLRRAVDADIPVLGVCYGHQLLAH
                     ALGGRVGYNPRGREMGSVRIERSSTTAPDALFDSAPLAFDAQATHLQTVLEPPRDAVV
                     LARSSLDDCQAVRFAPRAWGLQFHP"
     gene            complement(553..>2213)
                     /locus_tag="VFF05_09525"
     CDS             complement(553..>2213)
                     /locus_tag="VFF05_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HZW52091.1"
                     /translation="GKLELHPLVLVANQKLTNPQDNKPLSLEVLTHWLAEKAGLPYLK
                     VDPMKIDAAAVTQVVSHEYARRYRILPVAVSPMQVVIATSEPFDTRWVADLGHILRRD
                     IQRVVVSPIDLNRYLAEFYGVQRSIQKAKDAKLDGGMDGRAILNFEQLLELGKAGDIG
                     ADDRHVVHIVDWLLQYAFDQRASDIHLEPRRDMSPIRFRIDGVMHKVFELPTPVMVAV
                     TSRIKILGRMDLAEKRRPQDGRIKARSSEGREVEMRLSTMPTAFGEKVVMRIFDPDIV
                     VKEFRQLGFSTEEEAIWRQMVERPHGIVLVTGPTGSGKTTTLYSTLKHLATPDINLCT
                     IEDPIEMVSPEFNQMQVQPAIDLDFAAGVRTLMRQDPDIIMIGEIRDLETAQMAIQAS
                     LTGHLVLSTLHTNDAPSALSRLLDLGAPHYLIQSTLAGVVAQRLVRTLCPHCKAEAVL
                     DASGWDALVHRWRLPPPARVFAPVGCLECRNTGFLGRTGIYEMMRISPQLRQMIQPTL
                     DLARLGETALAEGMRPLRVSAAQQVARGVTTIAEVVGVLPPIEA"
BASE COUNT          392 a          719 c          719 g          383 t
ORIGIN      
        1 cgggatggaa ctgcagcccc caggcgcgcg gcgcaaaacg cacggcctgg caatcatcga
       61 ggctggatcg cgccagcacg accgcatcgc gcggcggttc gagcacggtt tgcagatgcg
      121 tggcctgcgc gtcgaacgcc agcggtgccg aatcgaacaa tgcatccggt gcggtggtgg
      181 acgagcgctc gatacgaaca ctgcccattt cgcggccgcg cgggttgtag ccgacgcgcc
      241 caccgagtgc atgcgccaac aactggtgac catagcacac gccaagcacg ggaatgtccg
      301 catcgaccgc gcggcgcagc caacgcgcgg tggcttcgct ccattgcaac cgatcggtga
      361 ccattgcacc ggagccggtg acgacgacgc cggacacgga tcgcggcggc ggcagttgtg
      421 cgccgcgatc gacgcgcatg cattgcgtct ggcgcggcga caagcctatg ctgcgtcgga
      481 accactgcgc gaaatcgcca tgttgccggc gcacggcagg aattgccgtt ccggtctgga
      541 cgatgagcag cttcatgcct ctatcggcgg cagcacgccg acgacttcgg cgatggtcgt
      601 cacgccgcgc gcgacttgct gcgcggcgct cacgcgcagc ggccgcatgc cttcggcgag
      661 cgcggtttcg cccaagcggg caaggtcgag cgtgggttgg atcatctgcc gcaactgcgg
      721 cgagatgcgc atcatctcgt agatgccggt gcggcccagg aagccggtgt tgcggcattc
      781 taggcaaccg accggcgcaa acacgcgcgc cggcggcggc aggcgccagc ggtgcacgag
      841 cgcatcccag ccgctcgcat ccagcaccgc ctccgccttg cagtgcgggc acagcgtgcg
      901 caccagtcgc tgcgcgacga cgccggcgag cgtggactgg ataaggtaat gcggtgcgcc
      961 caagtcgagc aggcgcgaca gcgcgctcgg tgcatcgttt gtgtgcaggg tcgacaagac
     1021 cagatggccg gtcaacgagg cctggatcgc catctgcgcg gtttccaggt cgcggatttc
     1081 gccgatcatg atgatgtcgg gatcctggcg catcagcgtg cgcacgccgg cagcgaagtc
     1141 gagatcgatc gccggctgca cctgcatctg gttgaattcc ggcgacacca tttcgatcgg
     1201 gtcttcgatc gtgcacaggt tgatgtcggg cgtggccaga tgcttgagcg tggaatacaa
     1261 cgtcgtggtc ttgccagaac ctgttgggcc cgtaaccagc acgatgccgt gcgggcgctc
     1321 gaccatctgc cgccagatcg cttcctcctc ggtggaaaag ccaagctggc ggaattcctt
     1381 gaccacgatg tccggatcga aaatgcgcat cacgaccttc tcgccgaacg cggtcggcat
     1441 cgtcgacagg cgcatttcca cttcgcggcc ttccgacgag cgcgccttga tgcgaccgtc
     1501 ctgcggacgg cgtttctcgg ccagatccat gcgcccaaga atcttgatgc gcgaggtgac
     1561 cgcgaccatc accggcgtcg gcaactcgaa aaccttgtgc atcacgccgt cgatgcggaa
     1621 gcggatcggg ctcatgtcgc ggcgcggttc cagatggatg tccgaggcgc gctggtcgaa
     1681 cgcgtattgc agcaaccagt cgacgatgtg cacgacgtgg cggtcgtcgg caccgatatc
     1741 accggctttt cccaactcca gcaactgctc gaagttcagg atcgcgcggc cgtccatgcc
     1801 gccatcgagt ttggcatcct tggccttctg gatcgagcgc tgcacgccgt agaactcggc
     1861 cagataccgg ttcagatcga tcgggctgac caccacgcgc tggatgtcgc gacgcaggat
     1921 gtggccaaga tcggccaccc aacgcgtatc gaacggttcg ctggtggcga tgacgacctg
     1981 catcggactg accgccaccg gcaggatgcg atagcgtcgc gcatactcgt gcgacacgac
     2041 ctgtgtcacc gccgcagcat cgatcttcat cggatcgact ttcaggtacg gcaggcccgc
     2101 cttttccgcc agccagtgcg tgagcacttc caggctcaac ggcttgttgt cctgcggatt
     2161 ggtcagcttc tggttggcga ccagcaccag cggatgcagc tccagcttgc cgc
//
LOCUS       DASRVO010000770         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_359594, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000770 DASRVO010000000
VERSION     DASRVO010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359594"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFF05_09530"
     CDS             <1..491
                     /locus_tag="VFF05_09530"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HZW52092.1"
                     /translation="TALSGDANSLGQRVFEFSEFVVKVAGIRDVGAYFPHRVAVHDAC
                     HALRELRIKEEPRELLRRVRGLELVEMPASEECCGFGGTFAVKFGMISAAMGDTKAGN
                     AEASGAEYVASTDPSCLLHIDGILRRRNSPVRTIHVASILAQTGRAKESVEIAARARS
                     AE"
     gene            488..1912
                     /locus_tag="VFF05_09535"
     CDS             488..1912
                     /locus_tag="VFF05_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HZW52093.1"
                     /translation="MTTASQEFLRAAARESANLPRRQAIRRAIDQYDAAVQRGREQFL
                     DYEAARRKCYEIKWEALNHLDRYLEEFEQRVRARGGQVFWAERAEDARRYVVDLATAR
                     GVRTVVKSKSMVAEEIHLTPALEKLGARVFETDLGEFIVQLRNEPPFHIVTPAMHLTR
                     ADIAKLFEEKLAEAMSPAASRDDPAELVAAARRALRRAFFSAEMGITGANFLVADAGL
                     IAITTNEGNDRLGTALPRIHVAIAGIEKVIPRLEDLAVLWPVLATAGTGQTITCYNTL
                     IGGPRAPAEVDGPPEFHVVLLDNGRTDLLADAEQRDVLHCIRCGACLNACPVFRSIGG
                     HAYGTTYSGPIGSVLTPHLRGLNEFQHLSYASSLCGACTTVCPVQIDLHHHLLHNRRN
                     AVRASDRPWSERLAFRFWRWTMSGSRRFALAGWLGRATLRGVRALGLAGTTLDLLRPW
                     TRYHAAPVIPRKSFRALWRKNGAR"
     gene            2007..>2523
                     /locus_tag="VFF05_09540"
     CDS             2007..>2523
                     /locus_tag="VFF05_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HZW52094.1"
                     /translation="MSVADPLERFQQQCALTSTECMLVPDAAAAAEALRTVLESIPAG
                     EIFAQDAPLLHRIVQRAEGGRAVRWSNAGPPNEGSQATITLAECLVASHGSVLIASNG
                     AGRAASVAAPVHIVLAGESQLVPDLASALARAKQDRLAETNSCLFLITGSSRTADIEK
                     ILVLGAHGPRRL"
BASE COUNT          420 a          753 c          910 g          440 t
ORIGIN      
        1 gcacggcgct gagcggcgac gcaaacagcc tgggccaacg cgtgttcgaa ttttcggaat
       61 tcgtggtgaa ggtggccgga atccgcgatg tgggcgcgta ttttccgcat cgcgtcgccg
      121 ttcatgacgc ttgccacgcg ctgcgcgaac tgcgcatcaa ggaagaaccg cgcgagctgt
      181 tgcggcgtgt gcgcgggctc gaactggtgg agatgccggc cagcgaggag tgctgcggat
      241 ttgggggcac gttcgcggtg aagttcggga tgatttccgc agcgatgggg gataccaagg
      301 cgggcaacgc ggaggcctcc ggcgcggaat acgtggcgtc gaccgacccg agctgcctgc
      361 tgcacatcga cggcattctg cgccggcgca actcgcccgt gcgcacgatt cacgtggcca
      421 gcatcctggc gcaaaccggg cgcgcgaagg agagcgtcga aatcgccgcg cgggcgcgga
      481 gcgctgaatg accaccgcgt cacaggaatt cctgcgcgcg gcggctcggg agtccgcgaa
      541 tcttccgcgg cgccaggcga ttcgccgggc gatcgaccag tacgatgcag ccgtgcagcg
      601 cggccgcgag cagtttctcg attacgaagc ggcgcgccgg aaatgctacg agatcaagtg
      661 ggaggcgctg aatcatctcg accgctatct ggaggaattc gagcagcgcg tgcgggcgcg
      721 cggcgggcag gtgttttggg cggagagagc ggaagacgcg cggcggtacg tcgtcgattt
      781 ggcgacagcg cggggagtgc gcacggtcgt caagtcgaaa tcgatggtgg cggaggaaat
      841 tcacctgacg cccgcgctgg aaaagctggg tgcgcgcgtg ttcgaaacgg acctgggcga
      901 gttcatcgtg caactgcgca acgagccgcc tttccacatc gtcacgccgg cgatgcacct
      961 gacgcgcgcc gacatcgcga aactgttcga ggaaaaactg gcggaggcga tgtcgcccgc
     1021 agcgtcgcgc gacgatcccg ctgaactggt ggctgccgcg cgacgggcgc tgcgccgcgc
     1081 gttcttttcc gccgagatgg ggattaccgg agcgaatttt ctggtggccg acgcgggctt
     1141 gattgcgatc accacgaatg aaggcaacga tcggctgggg acagcgcttc cgcggattca
     1201 tgtggccatc gcgggaatcg aaaaagtgat tccgcgactg gaggacctgg ccgtgctctg
     1261 gcccgtgctg gctacagcgg gaacggggca gaccatcacc tgttacaaca cgctgattgg
     1321 cgggccgcgc gcgccggcag aggtggatgg ccccccggag tttcacgtgg tgctgctcga
     1381 caacggccgc accgatttgc tggcggacgc cgagcagcgc gacgtcctgc attgcattcg
     1441 ctgcggggcg tgcttgaatg cgtgtcctgt gttccgctcg attggcggac acgcgtacgg
     1501 cacgacgtac tcggggccga tcgggagcgt gctgacgcct catctgcgcg ggctgaacga
     1561 gtttcagcat ctttcctacg cgtcatcgct gtgcggggcc tgcaccaccg tgtgcccggt
     1621 ccaaatcgat ctgcaccatc atttgctgca caaccggcgc aatgccgttc gcgcgagcga
     1681 ccggccatgg agcgagcgcc tggcgtttag gttctggcgg tggacgatga gcggttcgcg
     1741 acgttttgcg ctggcgggat ggctcgggcg cgccactctg cgcggcgttc gggcgctggg
     1801 gctggcggga acgacgctcg acctgctgcg accctggacc agatatcacg cggctccggt
     1861 tattccgcgc aaatcgtttc gcgcgctgtg gaggaagaat ggagcgcgct gaaaatccgg
     1921 ggcgcgagcg catccttgcg cgcatccatt cggcgctgag agcgagcgcg ccgcggcatg
     1981 atgcgggcga aaagcgcgcg attttcgtgt ccgttgccga tccgctcgaa cggtttcagc
     2041 agcagtgcgc gttgacttcg accgaatgta tgctcgtgcc cgacgcggcg gcagcagccg
     2101 aggctcttcg aaccgtgctg gaatcgattc cagctgggga aattttcgcc caggacgcgc
     2161 cgttgctgca tcggatcgtg cagcgggccg agggcgggcg cgcggtgcgc tggtcgaatg
     2221 caggtccgcc gaatgagggc tcgcaagcca cgattacgct cgcggagtgc ctggtggcgt
     2281 cgcacggctc ggtgctgatc gcgtcgaacg gcgcagggcg ggccgcttcg gtggctgcgc
     2341 cggtgcatat cgtgttggcc ggcgaatcgc agctggtgcc cgatctcgca tccgcgcttg
     2401 cgcgcgcgaa acaagaccgg ctcgccgaaa cgaattcctg cctgttcctg atcacgggat
     2461 cgagccggac cgcggatatt gaaaagattc tggtactggg tgcgcacggc ccgcggcggc
     2521 tgg
//
LOCUS       DASRVO010000771         1195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_334882, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000771 DASRVO010000000
VERSION     DASRVO010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334882"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1195)
                     /locus_tag="VFF05_09545"
     CDS             complement(<1..>1195)
                     /locus_tag="VFF05_09545"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZW52095.1"
                     /translation="DLYQLTMLQGYFERGMNGTAAFEFFARKLRPGRGFLLAAGLDQL
                     LDFVEQARFVPQELDWLRGTGKFSHEFVDWLAAWHFEGDIDAMPEGTVCFPDEPLLRV
                     TAPLPQAQLIETRLINLIHFQTLIASKAARSVLAAPGKLLVDFGLRRAHGAEAGLLAA
                     RAAYLAGYSGTATVLAGQRFGIPMFGTMAHSFVQAHDDEADAFRSFVHAQPDNVVLLI
                     DTYDTEAAAHKVVALARELEREGIAIKGVRLDSGDLGYLAHQVRRILDDGGLQATTIF
                     ASGDLDEYRLRELLVRGAPIDGFGIGTKLDTSADAPFIDCAYKLEEYAGRPRRKRSIG
                     KATWPGRKQVWRRYDESGRMAGDVVSLEHDKQPGEPLLQPAMRGGRRLAQPVLEQARH
                     HAAANL"
BASE COUNT          204 a          395 c          399 g          197 t
ORIGIN      
        1 caaattggcg gcagcgtgat gacgcgcctg ctccagtacg ggttgcgcca gccggcggcc
       61 gccgcgcatc gccggctgca gcagcggttc gcccggctgt ttgtcgtgtt ccaggctgac
      121 cacgtcgccc gccatgcggc cagactcgtc atagcggcgc catacctgct tgcgccccgg
      181 ccaggtggcc ttgccgatgg aacgcttgcg gcgcggcctg cccgcgtatt cttcgagctt
      241 gtaggcgcag tcgataaacg gcgcgtcggc ggaagtgtcg agcttggtgc cgatgccgaa
      301 gccgtcgatc ggtgcgcccc gcaccagcag ttcgcgcagg cggtattcgt cgaggtcgcc
      361 gctggcgaag atggtggtgg cttgcagccc gccgtcgtcg aggatgcgcc gtacctgatg
      421 cgcgagatat cccaggtcgc cgctgtcgag gcgcacgccc ttgatcgcaa tgccctcacg
      481 ttccagctcg cgcgccagcg ccaccacctt gtgcgcggcg gcttcggtat cgtaggtatc
      541 gatcagcagc accacattgt ccggctgcgc atgcacaaag ctgcgaaagg catcggcctc
      601 gtcgtcgtgc gcctgtacga aggaatgggc catggtgccg aacatgggaa tgccgaaacg
      661 ctgccccgca agcaccgtgg ccgtaccgga atagccggca aggtaagccg cgcgcgccgc
      721 gagcaggccg gcttcggcgc cgtgcgcgcg acgcaggccg aaatccacca gcagcttacc
      781 gggtgccgcc agcaccgagc gcgcggcttt ggaggcgatc agcgtctgga aatgaatcag
      841 gttgatcaag cgcgtctcga tcagttgcgc ctgcggcagc ggtgccgtca cgcgcagcag
      901 cggttcgtcc ggaaagcaca ccgtcccctc gggcatcgca tcgatatcgc cctcgaaatg
      961 ccaggcggcg agccagtcga cgaactcgtg cgaaaacttt ccggtgccgc gcagccagtc
     1021 cagttcctgc gggacgaagc gcgcctgttc gacgaaatcc aataattgat cgagccctgc
     1081 ggcgagcagg aaaccgcgcc cgggccgcag cttgcgcgcg aagaattcga acgcggcggt
     1141 gccgttcatg ccgcgctcga agtagccctg cagcatggtg agctggtaga gatcg
//
LOCUS       DASRVO010000772         1236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_386372, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000772 DASRVO010000000
VERSION     DASRVO010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386372"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VFF05_09550"
     CDS             complement(<1..901)
                     /locus_tag="VFF05_09550"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZW52096.1"
                     /translation="MNSTSNQRVAILGGVRIPFCRNNTAYADVGNFGMAVKTLGALVE
                     RFGLHGVELGDVAFGAVIKHASDWNLAREATLSSGLAPTTPGITTARACGTSLDNAII
                     IANKIASGQIEAGIAGGADTTSDVPIVYSRALQQRLLRVNRARGGWEKFKTAIGGFSL
                     SELKPSFPGVMEPRTGMSMGDHCELMAREWKISRQDQDAFALTSHQKAAAAYTRGFFN
                     DLVVPFRGLDRDNILRPDTTLEKLAMLKPAFDKTSGQGTLTAGNSTALSDGASAALLA
                     SDAWAVERGLKPLAYLTHAKVAAV"
     gene            complement(1000..>1236)
                     /locus_tag="VFF05_09555"
     CDS             complement(1000..>1236)
                     /locus_tag="VFF05_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52097.1"
                     /translation="LDLAQNANVPIIPYWVEFDLARCTRRLCIGAPLDPADTSGTLQA
                     LADILDRQIRATPSAWYFWRELPEWIAAAAKARE"
BASE COUNT          205 a          401 c          397 g          233 t
ORIGIN      
        1 ccaccgccgc gaccttggcg tgggtcaggt aggccagtgg cttgagtccg cgttcgactg
       61 cccaggcatc gctcgccagc aacgccgcgg aagcgccgtc cgacagcgcc gtggaattgc
      121 ccgccgtcag cgtgccctgc cccgaagtct tgtcgaacgc gggcttgagc attgcgagct
      181 tttccagcgt cgtgtccggg cgcaggatgt tgtcgcggtc caggccgcgg aacggcacga
      241 ccagatcgtt gaagaacccg cgcgtgtacg ccgccgcggc tttctggtgg ctcgtcagcg
      301 cgaatgcgtc ctgatcctgg cgcgagatct tccattccct ggccatcagc tcgcagtggt
      361 cgcccatcga catgccggtg cgcggttcca tcacgccggg gaaactgggc ttgagctcgg
      421 acaacgaaaa tccgccgatg gcggtcttga atttttccca gccgccgcgg gcgcgattga
      481 cgcgcagcag gcgctgctgc agggcgcggc tgtaaacgat gggcacgtcg ctggtggtat
      541 ccgcaccgcc ggcaatgccg gcttcgatct ggccggatgc gatcttgttg gcgatgatga
      601 tggcgttgtc gagcgacgtg ccgcaggcgc gcgccgtggt gatgcccggc gttgtcggcg
      661 ccagccccga cgacagcgtc gcctcgcgcg cgaggttcca gtcggaagca tgcttgatga
      721 ccgcgccgaa cgcgacatcg cccaattcca cgccatgcag gccgaacctt tcgaccaatg
      781 ccccgagcgt cttcactgcc atgccgaaat tgccgacgtc ggcatacgcg gtattgttgc
      841 ggcagaacgg aatgcggact ccgccgagga tggcgacgcg ttggttcgag gtcgagttca
      901 ctgcgtcttc cttcacaatg tgggatcgcc gttccggcgc gccgcagcgt gtcggacggc
      961 tttattgtat gccgactgcc tgcgcttgtg cgcggtaaat cactcccgtg ctttcgccgc
     1021 agccgcgatc cattccggca attcccgcca gaaataccac gcactcggcg tggcgcggat
     1081 ctggcgatca agaatatcgg ccaacgcctg caacgtaccg ctcgtatcgg ccggatccag
     1141 cggcgcgccg atgcatagac gacgcgtgca gcgggcaaga tcgaattcca cccaatatgg
     1201 aattatcggc acgttcgcgt tctgcgcaag atcgag
//
LOCUS       DASRVO010000773         1291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_49772, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000773 DASRVO010000000
VERSION     DASRVO010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49772"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..>1291
                     /gene="pepQ"
                     /locus_tag="VFF05_09560"
     CDS             219..>1291
                     /gene="pepQ"
                     /locus_tag="VFF05_09560"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HZW52098.1"
                     /translation="MSDLLAKLYPDHLRTLQARADAALARGGFDHLVVAAGVSRYEFL
                     DDRPAPYVANPHFKHWLPVTKAPGSWLVYTPGARPKLIYLQPRDYWHVVPEAPAGYWV
                     EHFDIVIVHEGRDALAQLPKALARCAIIGADNAALGDAKPNNPERVLNYLHWQRSYKT
                     DYELAMLRVASKLGVRAHRAAEAAFRAGRSEYDINTAYLNAVHETENELPYGNIIALN
                     QHAATLHYMDLNRAPPTHAESFLIDAGASFHGYGSDITRTHAASHAGEFQALIDAVDT
                     AQRGFVKMVRKGQNYADLHTHAHHVLAGILREQGFIRLTAESAVETGVSSTFFPHGLG
                     HMLGLQVHDVAGFQKDENGGTIPR"
BASE COUNT          246 a          432 c          399 g          214 t
ORIGIN      
        1 aagagccaca cgctcgaaat cggcctgcat gaacaatggt gcgaaacggc caatgtgccc
       61 ggccagttct ggaccggttt tcgcatcatc gacatcgggc cggaggatta caacgtcctg
      121 ttcgactggg tcaattcgcc gggcggccag ttcgattaag cgccgctgaa actccatctg
      181 cggcacaata ggcgcatcgc gttgatcggt gcgccgccat gtccgacctg ctggccaaac
      241 tctatccgga tcacctgcgc acgctgcaag cgcgcgccga cgcggcgctc gcgcgcggcg
      301 gttttgatca tctggtcgtc gccgcgggcg tgtcccggta cgagtttctc gacgaccgac
      361 ccgcgcctta cgtcgcaaat cctcatttca agcactggct gccggtgacc aaggcgccgg
      421 gttcgtggct cgtgtatacg cccggcgcga gaccgaaact gatttacctg cagccacgcg
      481 attactggca cgtggtgccg gaagcgccgg ccggttactg ggtcgagcac ttcgacatcg
      541 tcatcgtgca cgaggggcgc gacgcgctcg cgcagctgcc caaggcgctg gcgcgctgcg
      601 ccataatcgg cgcggacaac gccgcgctcg gcgacgcgaa accgaacaat ccggaacgcg
      661 tgctcaatta cctgcattgg cagcgcagct acaagaccga ctacgagctg gccatgctgc
      721 gcgtggcgag caagctcggc gtgcgtgcgc accgcgccgc ggaagccgcg tttcgcgcgg
      781 ggagatccga gtacgacatc aacacggcgt atctcaacgc cgtgcacgaa accgaaaacg
      841 agctgcccta cggcaacatc atcgcgctca atcagcacgc agcgacgctg cactacatgg
      901 atttgaatcg cgcgccgccc acgcatgccg aatcgttcct catcgacgcc ggcgcgagtt
      961 tccacggcta cggctcggac atcacgcgca cccatgccgc gtcgcacgcg ggcgaattcc
     1021 aggcattgat cgacgccgtc gacacggcgc agcgcgggtt tgtgaaaatg gtgcgcaagg
     1081 ggcagaacta cgccgacctg cacacccacg cgcaccacgt gctggccggc atcctgcgcg
     1141 agcagggctt catccgcctg actgcggaaa gcgcggtgga aaccggcgtg tcgtcgacgt
     1201 tctttccgca cggcctcggc cacatgctcg gtttgcaggt gcacgacgtc gccggtttcc
     1261 agaaggacga gaatggcggc acgattccac g
//
LOCUS       DASRVO010000774         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_22895, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000774 DASRVO010000000
VERSION     DASRVO010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22895"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFF05_09565"
     CDS             complement(<1..265)
                     /locus_tag="VFF05_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HZW52099.1"
                     /translation="MVDCQWACPAHTPVPEYIRLIAAGRHDDAYLINWKSNVFPGILG
                     RTCDRPCEPACRRGRVEENNGADPDKSRPEPVAICRLKRVCADN"
     gene            complement(378..770)
                     /locus_tag="VFF05_09570"
     CDS             complement(378..770)
                     /locus_tag="VFF05_09570"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZW52100.1"
                     /translation="MQQATHLEPVFHPFGTIPGLPFSEAVEVGDLLFLSGQIGTDTNG
                     KLVAGGIADETRHALANIGRVLARHGSAMERVVKVTVMLADMAEWPAMNAEYVKFFAK
                     NPPARSAFGCNGLAMKARVEIECVALRK"
     gene            862..2103
                     /gene="phaZ"
                     /locus_tag="VFF05_09575"
     CDS             862..2103
                     /gene="phaZ"
                     /locus_tag="VFF05_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HZW52101.1"
                     /translation="MLYRLHEFNRNLFNPLLAWSQTSAKMLTSPDSWLSQLPFAQRAA
                     ANCELVYRLGKEYEKPAFGIHTVNAHGQDVAVLERVALAKPFCNLLRFKRFSDDVPTL
                     AAMKNDPTVLVVAPLSGHHATLLRDTVKSLLPEHKVFITDWLDARMVPAEAGPFHLDD
                     YVAYMREFIAHIGAKNLHVISVCQPTVPVLAAVSLMASNGEALPRTITLMGGPIDTRC
                     SPTSVDDLATHKSPSWFENTLVQEVPDNYPGRGRRVYPGFLQHAGFLAMNPGRHFMSH
                     WDFYENLCRGDQEDADAHRRFYDEYNAVLDMPAEYYLDTIRIVFQEHALPLGTWDVAG
                     ELVRPQDIRDTALFTIEGELDDISGLGQTRAAHDLCTNLPAAAKRHLTVEGAGHYGIF
                     SGRRWRNGVYPQVRDFIRAHG"
BASE COUNT          392 a          726 c          656 g          388 t
ORIGIN      
        1 tgttgtcggc gcagacgcgc ttgaggcggc agatcgctac cggttccggc cggctcttat
       61 caggatcggc accgttgttt tcctcgacgc gtccgcgccg gcacgccggt tcgcagggac
      121 gatcgcaggt gcggccgagg attccgggaa agacgttcga tttccagttg atcaggtagg
      181 cgtcgtcgtg acgcccggcc gcgatcaggc gaatgtactc gggaaccggg gtgtgcgcgg
      241 gacaagccca ctggcaatcg accactttgt gaaagtagtc gggaacgcga atatccgtag
      301 gcttcaccct gacctccgcg atggcatttt cgttccgagt ctactcgggc acgcctcggc
      361 tgagaaggcg cgcgaactta cttgcgcagc gcgacgcatt cgatctcgac acgcgccttc
      421 atcgccagtc cattgcagcc gaacgcgctg cgcgccggcg ggttcttcgc gaagaatttg
      481 acatactccg cgttcatcgc cggccattcg gccatgtcgg cgagcatcac agtcaccttg
      541 accacgcgct ccatcgccga accgtggcgt gcgaggacac ggccgatgtt ggccagcgcg
      601 tggcgggtct cgtcggcaat gccgccggcg acgagcttgc cgttcgtgtc ggtgccgatc
      661 tgtccggaca agaacagcag gtcgcccact tccacggctt cggaaaacgg cagcccggga
      721 atcgtgccga acggatggaa cacgggttcg aggtgcgtgg cttgttgcat gatcgctctc
      781 cgctggattg gcggtcgcat tgcagcacaa attccgccac aatgcgccgt cgccggcgct
      841 cccggccctg ccggaatgtc gatgctttac cgcctgcacg aattcaaccg caacctgttc
      901 aacccgctgc tggcctggtc gcaaaccagc gccaagatgc tcacctcccc cgacagctgg
      961 ctctcgcaac tgcccttcgc gcaacgcgcc gccgccaatt gcgaactcgt ctaccggctc
     1021 ggcaaggaat acgaaaagcc cgccttcggc atccataccg tcaacgcgca cggccaggac
     1081 gtcgccgtcc tcgaacgcgt ggcgctggca aaacccttct gcaacctgct gcgcttcaag
     1141 cgcttcagcg acgacgttcc cacgctggcg gcgatgaaga acgatcccac cgtgctggtc
     1201 gtcgcgccgc tgtccggcca tcacgccacc ttgttgcgcg acaccgtgaa gtccctgctg
     1261 cccgagcaca aggtgttcat caccgactgg ctcgatgcgc gcatggtgcc ggccgaagcc
     1321 gggccgttcc acctcgacga ttacgtcgcc tacatgcgcg agttcattgc ccacatcggc
     1381 gcaaaaaacc tgcacgtgat cagcgtctgc cagccgaccg tgcccgtact ggccgcggtt
     1441 tcgctgatgg catccaacgg cgaggcgctg ccgcgcacca tcaccctgat gggcggaccg
     1501 atcgacacgc gttgcagccc gaccagcgtg gacgacctgg ccacgcacaa gtcgccttcg
     1561 tggttcgaga acacgctcgt gcaggaagtg ccggacaact accccggccg cggccgccgc
     1621 gtgtacccgg gattcttgca gcacgccgga ttcctcgcca tgaatccggg ccggcatttc
     1681 atgtcgcact gggatttcta cgagaacctt tgccgcggcg accaggaaga cgccgacgcg
     1741 caccgccgct tctacgacga atacaacgcc gtgctcgaca tgccagccga gtactacctc
     1801 gacacgatca ggatcgtgtt ccaggaacac gccctgccgc tgggcacctg ggacgtcgcc
     1861 ggagaacttg tgcggccgca ggacatccgc gacacggcgc tgttcaccat cgaaggtgag
     1921 ttggacgata tctcagggct gggacagacg cgtgctgcac acgatctgtg cacgaacctg
     1981 cctgccgcgg cgaagcgtca tctgacggtg gaaggcgctg gccactacgg aattttcagc
     2041 gggcggcgct ggcgcaatgg cgtgtatccg caggtgcggg atttcatccg cgcacatggc
     2101 tgaagcaagc taacgcctga attaagccgc gccgcgaagc ggcgtcggct tgtatgcatt
     2161 gt
//
LOCUS       DASRVO010000775         1187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_100267, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000775 DASRVO010000000
VERSION     DASRVO010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100267"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFF05_09580"
     CDS             complement(<1..312)
                     /locus_tag="VFF05_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52102.1"
                     /translation="MSRIAAIAASSLITSALIASAAAFAQTAPAPLTLDRITLEQIMA
                     DPDWIAGQIQVPEEFEEGGGAPYLSIDGRSVYYKLKRNGSPVFDLHRIDLADGKDSLI
                     DA"
     gene            444..1106
                     /locus_tag="VFF05_09585"
     CDS             444..1106
                     /locus_tag="VFF05_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HZW52103.1"
                     /translation="MPLPCIEKTTGPNPRHAVIWLHGLGADGNDFLPIVPELVDPKWP
                     PLRFVFPHAPMRPITINGGMAMRAWYDISGQEIAQRQDETGIRSSIVLLEELVAREVE
                     RGIACENIFLAGFSQGGAIVLAGGIRHAQRLGGIVALSTYLPMAEKTDAEASVANRTT
                     PIFMAHGMLDPTISHALGEMSRAYLVQRGYAIDWHAYQMAHQVCMEEIADLRVWLSTR
                     LV"
BASE COUNT          218 a          373 c          386 g          210 t
ORIGIN      
        1 ggcgtcgatg agactgtcct tgccgtcggc aagatcgatg cggtgcaggt cgaacaccgg
       61 cgagccgttg cgtttgagtt tgtagtacac cgatctgccg tcgatgctga ggtacggcgc
      121 gccgccgcct tcctcgaatt cctcgggtac ctggatctgc ccggcgatcc agtccggatc
      181 ggccatgatc tgttcgagcg tgatccgatc gagcgtgagc ggcgccggcg cggtctgcgc
      241 gaacgcggcg gccgaggcga tgagggccga tgtgatcagg gacgatgcgg caatggcggc
      301 gatgcgggac acgggcgttt cctcgaatgc aaacgcgcag catagcggct gggcgcggca
      361 tcgcaagccg ccacaagtca tggcgcgctg gcagatatgg gccaaatccg gcaccatgct
      421 cgcgccgccg ccaaggatcc gccatgcccc tgccctgcat cgaaaagacc accgggccga
      481 acccgcgtca cgccgtgatc tggctgcatg gcctgggcgc cgacggcaac gatttcttgc
      541 cgatcgtgcc ggaactggtc gatccgaaat ggccgccatt gcgcttcgtg ttcccgcacg
      601 cgcccatgcg cccgatcacg atcaacggcg gcatggcgat gcgcgcctgg tacgacatct
      661 ccggccagga aatcgcgcag cgccaggacg agaccggcat ccgttcctcg atcgtcttgc
      721 tcgaggaact ggtcgcgcgc gaagtcgaac gcgggattgc atgcgaaaac atcttcctcg
      781 ccggattctc gcagggcggt gcgatcgtgc tcgccggcgg catccgtcat gcgcagcgcc
      841 tcggcggcat cgtcgcgttg tccacctacc tgccgatggc ggaaaagacc gacgccgagg
      901 caagcgtggc gaatcgcacc acgccgatct tcatggcgca tggcatgctc gatccgacga
      961 tttcgcacgc gcttggcgag atgagccgtg cgtatctcgt gcagcgcggc tacgcgatcg
     1021 actggcatgc gtatcagatg gcgcatcagg tgtgcatgga agaaatcgcc gatttgcggg
     1081 tttggctgtc cacccgattg gtttgaccat gattcaaacc catttcaaaa tcgtcacgag
     1141 cgaagacaga ttgcacgtcg ccggagcgca ggaaccggag catacag
//
LOCUS       DASRVO010000776         1087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_231025, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000776 DASRVO010000000
VERSION     DASRVO010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231025"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /gene="glmM"
                     /locus_tag="VFF05_09590"
     CDS             <1..200
                     /gene="glmM"
                     /locus_tag="VFF05_09590"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974558.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZW52104.1"
                     /translation="AAAIKHASVQAALRQAEETLRGRGRVVLRASGTEPLVRVTVEGE
                     DAAEVRRLAQELARVVESALS"
     gene            283..>1087
                     /locus_tag="VFF05_09595"
     CDS             283..>1087
                     /locus_tag="VFF05_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HZW52105.1"
                     /translation="MANRVPTLDIRRFDSDRSAFVAELGAAYREWGFAGIRGHGIPQA
                     LIDGAYDAFVRFFALPAETKAKYHLKGTGGARGYTPFGVETAKDSRYPDLKEFYHIGR
                     ELPPGSKFADVMAPNVWPAEISDFHDKGLALYNALDALGSRVLRGLALHIGLAEDFFA
                     DKTNFGNSILRPIHYPPITTPDVPNVRAGAHEDINFITLLVGASSAGLEVKSRAGEWV
                     PFTSDADTIVVNIGDMLQRLTNHVYPSTTHRVVNPPGEQARKPRYSVPFF"
BASE COUNT          201 a          361 c          335 g          190 t
ORIGIN      
        1 ccgccgccgc gatcaagcac gcgtcggttc aagctgcgct gcggcaggcg gaagaaaccc
       61 tgcgcgggcg cggccgcgtg gtgctgcgcg cctccggcac cgaaccgctg gtgcgcgtga
      121 ccgtcgaagg cgaggacgcc gcggaagtgc gtcggctcgc acaggagttg gcgcgcgtcg
      181 tagaatcggc cttgtcatag caatcatcct tgattgcgaa gatcaaaaag cagccgtcct
      241 tggccgcact tttcaaaaaa acatctggca cccataaaag ctatggccaa tcgtgttccc
      301 accctcgaca tccgccgctt cgacagtgat cgcagcgctt tcgtcgccga actcggcgcg
      361 gcctaccgcg aatggggctt tgccggcatc cgcggccacg gcattccgca ggcattgatc
      421 gacggcgcct acgacgcatt cgtgcgcttc ttcgcgctgc ccgccgaaac gaaggcgaag
      481 taccacctca agggcaccgg cggcgcgcgc ggctacacgc cgttcggcgt ggaaacggcg
      541 aaggattcgc gctatccgga tctgaaggag ttctaccaca tcggccgcga actgccgccc
      601 ggctcgaaat tcgccgacgt gatggcgccg aacgtgtggc cggccgagat ttcggatttc
      661 cacgacaagg gattggcgct gtacaacgcc ctcgatgcgc tcggctcgcg cgtgctgcgc
      721 ggactcgcgc tgcatatcgg gttggcggag gattttttcg ccgacaagac gaatttcggc
      781 aattcgatcc tgcgcccgat ccattatccg ccgatcacga cgccggacgt gcccaacgtg
      841 cgtgccggcg cgcacgaaga catcaacttc atcaccttgc tggtcggtgc gagcagtgca
      901 gggctggaag tgaaatcgcg cgcgggtgag tgggtgccgt tcacgtccga cgccgacacc
      961 atcgtcgtca acatcggcga catgctgcaa cgcctgacca accacgtgta tccctcgacc
     1021 acgcatcgcg tcgtcaatcc gccgggcgaa caggcgcgca agccgcgcta ttcggtgccg
     1081 ttcttcc
//
LOCUS       DASRVO010000777         1247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_283903, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000777 DASRVO010000000
VERSION     DASRVO010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283903"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VFF05_09600"
     CDS             complement(<1..334)
                     /locus_tag="VFF05_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZW52106.1"
                     /translation="MDDVDVLIVGGGLVGASLAIALDGAGLRVALAEAAPPKVDLQPS
                     DDERNLALARATVNALDALRVWAHCGAKATAIRKIHVSRQGEFGVVRLEAAKHGVDAF
                     GATLPAREL"
     gene            371..976
                     /locus_tag="VFF05_09605"
     CDS             371..976
                     /locus_tag="VFF05_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZW52107.1"
                     /translation="MDLILVRHGDVEAAPGLCLGQTDAPLSASGFGAVQQLAATWTQG
                     APRFLFSSDLKRAQQAAQIFAARFAIEPLFDARLREVNLGDWDGKRWEDVMRSDAARY
                     EHWSANWVIQEAPGGESFADAIRRTGAWLASVLSSTHDEDTVLAVAHAGSIRALLCHA
                     LGLPAQRAYALRIDHAHAARIRTRAGQFEVCYVNASRFESD"
     gene            1002..>1247
                     /locus_tag="VFF05_09610"
     CDS             1002..>1247
                     /locus_tag="VFF05_09610"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HZW52108.1"
                     /translation="MPQFPQNHTVSPRTWVLAGMIAFAVGVRLLIYFVPGLFPYNFTP
                     VEAIALFGGAYFTNRRLAFAVPLVAMLCADLVIAAALP"
BASE COUNT          197 a          431 c          393 g          226 t
ORIGIN      
        1 ccagttcgcg cgcgggcaag gtcgcgccga aagcatccac accgtgtttc gccgcctcca
       61 gccggacgac accgaactcg ccctgccggc tgacatgaat cttgcggatc gcagtcgcct
      121 tcgcaccgca atgcgcccag acgcgcagcg catcgagcgc gttcacggtg gcacgggcga
      181 gtgcgagatt gcgctcgtca tcacttggct gcaaatcgac cttgggcggc gcggcctcgg
      241 ccagcgccac gcgcaaaccc gcgccgtcca gcgcgatggc caggctcgcg ccgaccagtc
      301 cgccgccgac gatgagaacg tccacgtcat ccatgcgccg atgatagcgc gagagtgcta
      361 acctgttcga atggacctca tcctcgtacg ccacggcgat gtcgaagccg cgcccggcct
      421 gtgcctgggc cagaccgacg cgcccttgtc ggcatcgggt ttcggcgccg tgcagcagct
      481 tgccgcgacc tggacgcaag gcgcgccgcg ttttttgttt tccagcgact tgaaacgtgc
      541 acaacaagct gcacagattt tcgccgcgcg cttcgccatc gaaccgctgt tcgacgcgcg
      601 cctgcgcgaa gtgaacctgg gcgactggga cggcaagcgc tgggaagacg tgatgcgcag
      661 cgatgcggcg cgttacgaac actggtcggc gaactgggtg atccaggaag cgccgggcgg
      721 cgagagtttc gccgacgcga tccgccgcac cggcgcctgg ctggcctcgg tgctgtcctc
      781 gacgcacgac gaggacacgg tgctggcagt ggcgcatgcc ggctccatcc gcgccctgct
      841 ttgccacgcg ctcggcctgc ccgcgcagcg cgcctacgcg ttgcgcatcg atcacgcgca
      901 tgcggcgcgc attcgtaccc gagccggtca gtttgaagta tgctatgtga acgcttctcg
      961 cttcgagtcg gattgaagcg ccgttttccc ggagttacac aatgccgcaa tttccacaaa
     1021 atcataccgt ctcgccgcgc acctgggtgc tggcgggaat gatcgcgttc gccgtcggcg
     1081 tacgcctgct gatctacttc gtgccgggcc tgttcccgta caacttcacc ccggtggaag
     1141 cgatcgcttt gttcggcggc gcgtatttca cgaatcgccg tctcgcattc gcggtgccgc
     1201 tcgtcgcgat gttgtgcgca gacctcgtca tcgccgcggc gctgccg
//
LOCUS       DASRVO010000778         1884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_335236, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000778 DASRVO010000000
VERSION     DASRVO010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335236"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VFF05_09615"
     CDS             complement(<1..778)
                     /locus_tag="VFF05_09615"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HZW52109.1"
                     /translation="MRKSRIFVCLIALLPAGAALADEPLVLPTAEQAPSAWSWFGDVL
                     LREDHVRDVPRTHEPNIQRVFGRGRFGVLYDPIPQLEIGAAIKLAAASNGNEDDRSYN
                     LDERSNDIAIDQFFLRWRANENTSLLAGKAVFPLELSPMLWDEDLRPLGVSAQTSVAV
                     GDFDRLSLVAGYFNGNLPYGDDSRIAAVQGAWRWHEGGPTSASILVSYLDFSSLRQLT
                     LQGLARSNVHTGDVLLSDYRLLDAQFVGRWDLGNWPLEARL"
     gene            complement(848..>1884)
                     /locus_tag="VFF05_09620"
     CDS             complement(848..>1884)
                     /locus_tag="VFF05_09620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HZW52110.1"
                     /translation="RQSGDELKARNGEAMLTAAVGVVSGKRFYALSEAALRDLPQAPM
                     PDGADAPTIVPAAKPHSRPSGVRAVALDTGAVVGGRYRILAQLGAGGMGIVYKAHDLE
                     LDDVVALKMLRPSALVDGEQLDRLKSEIKLARKITHPNVLRTFDFGEVDGQPFISMEY
                     VRGLTLRYLLSETRRIPYTAGLRIARQLCAGLAAAHEVGVLHRDIKPENLILEQNGNA
                     KLMDFGIARPIRRSGPGHTEQGSFVGTPNYSPPEQLAGEDVDQRADIYSSGVLMCEMF
                     CGKLPFSGSNTMEIYIAQTQQEPIKPSAYWPEIPPPMEAIILRCLKRLPQDRFASAAE
                     LAQALAQLRG"
BASE COUNT          353 a          610 c          599 g          322 t
ORIGIN      
        1 caagacgcgc ttccaacggc cagttgccca aatcccagcg gccgacgaat tgcgcgtcga
       61 ggaggcgata gtcgctgagc aggacgtcgc cggtgtgcac gttgctgcgc gccaggccct
      121 gcaaggtcaa ttggcgcagg ctcgagaaat ccagatacga aacaaggatg gacgcgctcg
      181 tcggcccgcc ctcgtgccag cgccaagcgc cctgcaccgc ggcaatccgc gaatcgtcgc
      241 cataaggcag attgccgttg aaatagcccg cgaccagact caggcgatcg aagtcgccga
      301 ctgcgacgga ggtctgcgca ctaacgccga gcggacgcaa gtcctcgtcc cacagcatcg
      361 ggctcagttc caggggaaag acggccttgc cggcgagcaa cgaggtgttt tcgttcgcac
      421 gccagcgcaa gaaaaactgg tcgatcgcga tgtcgttgct gcgttcatcg aggttgtagc
      481 tgcgatcgtc ctcgttgccg ttgctcgccg ccgcgagctt gattgccgcg ccgatttcca
      541 gctgcggaat cggatcgtag agcacgccga agcggccacg gccgaacacg cgctggatat
      601 tcggctcgtg cgtacgcggg acgtcgcgca catggtcctc gcgcaacaac acgtcgccga
      661 accacgacca tgcggaaggt gcctgctccg cggtcggcag cacaagcggc tcgtccgcca
      721 gggcggcgcc ggccggcagc agggcaatca ggcaaacgaa aatccgcgat ttgcgcatgg
      781 cctcccccgg tcgcacggcg tcgatggccg aatttacctt aaagatacat cacaagccaa
      841 tgaaaaatca gccgcgcaac tgcgccaatg cctgggccaa ctctgccgca gaggcgaaac
      901 ggtcctgcgg caggcgcttc aagcagcgca ggatgatggc ttccatcggc ggcgggattt
      961 ccggccagta ggcggacggc ttgatcggct cctgctgggt ctgggcgatg tagatttcca
     1021 tcgtgttgct gccggaaaac ggcagcttgc cgcagaacat ctcgcacatc agcacgccgc
     1081 tggaatagat gtcggcgcgc tgatcgacat cctcgcccgc gagttgttcg ggcggcgaat
     1141 aattcggagt gccgacgaag ctgccctgct cggtatgtcc gggaccactg cgccggatcg
     1201 gccgggcgat gccgaaatcc atcagtttgg cgttgccgtt ctgttcgagg atcaggtttt
     1261 ccggcttgat gtcgcggtgc agcacgccaa cctcgtgcgc ggcagcaagt ccggcgcaga
     1321 gctggcgcgc gatgcgcagg ccggcggtat acggtatgcg ccgcgtctcg ctgagcagat
     1381 agcgcaaggt caggccgcgc acgtattcca tcgagatgaa cggctggcca tcgacctcgc
     1441 cgaaatcgaa ggtgcgcagc acattcggat gcgtgatctt gcgcgcgagc ttgatttcgc
     1501 tcttgagccg atcgagttgt tcgccgtcca ccagcgccga ggggcgcagc atcttcaggg
     1561 cgacgacgtc atcgagttcc agatcgtgcg ccttgtagac gatgcccatg ccgccggcac
     1621 cgagctgcgc caggatgcgg tagcgcccgc caaccacagc ccccgtatcc agcgccaccg
     1681 cacgtacgcc ggatggacgg ctgtgcggtt tggccgcggg aacgatcgtg ggcgcatccg
     1741 cgccatcagg catcggcgcc tgcggcaaat cgcgcagtgc cgcttcggac aatgcgtaga
     1801 aacgcttgcc gctgaccacg cccaccgccg ccgtgagcat cgcctcgcca ttccgggctt
     1861 tcaattcatc gccgctctgt cgcg
//
LOCUS       DASRVO010000779         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_309790, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000779 DASRVO010000000
VERSION     DASRVO010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309790"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..519
                     /locus_tag="VFF05_09625"
     CDS             82..519
                     /locus_tag="VFF05_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52111.1"
                     /translation="MFVTAPLVSDVAPLARTPCHGSGRIVWQQRYEGIARVAYRGGKA
                     IAGVSGPWSDRYVLIWWDELPTSANPLELFETAEAAMLAVEKRMGQAMPEIAVTGEPR
                     REPGRSWWAGLWTKRRDRQTARLRRCHCDAEIDLSGLNFSASR"
     gene            complement(672..878)
                     /locus_tag="VFF05_09630"
     CDS             complement(672..878)
                     /locus_tag="VFF05_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52112.1"
                     /translation="MKCRLLFCGLLLCATAAFAQAASTWTATGTGTGSGGGVADSTTD
                     ANCTLDAALADSSLAIAQSRTKHR"
     gene            complement(973..2805)
                     /gene="glmS"
                     /locus_tag="VFF05_09635"
     CDS             complement(973..2805)
                     /gene="glmS"
                     /locus_tag="VFF05_09635"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014648482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZW52113.1"
                     /translation="MCGIVGAAARRDVVGTLIAGLKALEYRGYDSAGIALLTDTGLER
                     VRTKGKVHELETLHTSHPITGFTGIAHTRWATHGAPNTVNAHPMISRGNIAVVHNGII
                     ENHEELRAELKALGFAFDSDTDTEVIAHLIEHYQNQGADLLAAVQKTLPRLRGAYAIA
                     VISKREPGRVIGARRGSPMVAGLADGEQFIASDIHALLPVTRRFIYLAEGDIADATVD
                     GITIHDEHGHVVQRPIKQAHFGADAVERGEYRHYMLKEIHEQPQALADTLEGRISAGH
                     ILTEIFGVGARELLTKTRCVHLIACGSSYHAALVARYWIEALAGIPCNVEIASEYRYR
                     DAVVPADTLFVTISQSGETADTLAALKLAKSKNYLATLAVCNVPESSIVRESALTLMT
                     RAGPEIGVASTKAFTTQLAALALFALELARHHGLDAARYAALVQQLETLPGSVRDALA
                     LDERIKAMAERFVGKDHTLFLGRGTQFPIAMEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDENMPVIAVAPNDHLLEKLKSNLEEVRARGGELYVLADADVARNMRQDNGHLLAL
                     PPCGELVAPIVFTIPLQLLAYHVAVQRGTDVDQPRNLAKSVTVE"
     gene            complement(2862..>3057)
                     /locus_tag="VFF05_09640"
     CDS             complement(2862..>3057)
                     /locus_tag="VFF05_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HZW52114.1"
                     /translation="RTVIEDGAFIGSNSALVAPVTIGKDATIGAGSTIAADAPPGELT
                     VARARQRTVPNWKRPQKTPK"
BASE COUNT          502 a          941 c         1037 g          577 t
ORIGIN      
        1 cggtttcatc cacggtacgc cccattcggc gctggcatgc gccttgcttt tattcagaca
       61 acgcttcaca ggttgtctga aatgttcgtc accgcccccc ttgtttccga tgtcgcgcca
      121 ctggcgcgca ccccgtgcca cggctccggc cgcatcgtct ggcagcagcg ctacgagggc
      181 atcgcccgcg tcgcctatcg cggcggcaag gccatcgctg gcgtgtccgg gccgtggtcg
      241 gaccgttacg tgctgatctg gtgggacgag ctccccacgt cggccaatcc gctcgagctg
      301 ttcgaaaccg ccgaggcggc aatgctcgcc gtggaaaaac gcatgggtca ggcaatgccg
      361 gaaatcgccg tcacgggcga acctcgccgt gaaccgggac gcagctggtg ggccggtctt
      421 tggacaaagc gccgcgatcg gcagacagcg cggttgcggc gttgccattg cgacgccgag
      481 atcgacttga gcggcttgaa tttcagcgct tcgcgctgaa ctactcgaag ccgttgtaga
      541 aaatagaggt catgtcgaac gcgccgacgg tgcagccgat ggcgccaggc gaagtgcgcg
      601 cataaccgcg ttgatccagc gtgacagcgg gaatcacggc gtatccgttg ctgccatcgt
      661 tgccggcgca gttaccggtg cttcgtgcgg gactgagcga ttgcaaggct actgtcggcg
      721 agcgcggcgt ccagcgtgca attcgcgtcg gtcgtggaat cggccacgcc gccgccgctg
      781 ccggtgccgg tgccggtcgc cgtccaggta ctcgcggcct gcgcaaacgc cgcggtcgcg
      841 cacagaagca atccgcaaaa aagcagtcga cacttcatgg cgaatcctcg tcgggtgcgt
      901 tccacaaccg gcgcgaagcc ggttcttgtc acctttaacg cggaatcgag cgaaatccta
      961 tcgcgccgaa agctactcga ccgttaccga tttggccaga ttgcgcggct ggtccacgtc
     1021 ggtgccacgc tggacggcga cgtggtaggc gagcagctgc agcgggatcg tgaacacgat
     1081 gggcgcgacc aattcgccgc agggcggcag cgcaagcaaa tggccgttgt cctggcgcat
     1141 gttgcgcgcg acgtcggcgt cggccagcac gtagagttcg ccgccgcgcg cgcggacttc
     1201 ctcgaggttc gacttgagtt tttccagcag atgatcgttc ggcgcaaccg cgatcacggg
     1261 catgttttcg tcgacaaggg cgagcgggcc gtgcttgagc tcgcccgctg gatacgcctc
     1321 ggcgtggatg taggagattt ccttgagctt gagcgcgcct tccattgcga tcgggaactg
     1381 cgtgccgcgg ccgaggaaca gggtgtgatc tttgccgacg aagcgctccg ccatcgcctt
     1441 gatgcgttca tcgagcgcga gcgcatcgcg taccgaaccc ggcaaggttt ccagttgctg
     1501 cacgagcgca gcgtagcgcg cggcgtcgag gccatgatgg cgcgcaagct caagtgcgaa
     1561 caatgcgagc gcggccagtt gcgtggtgaa tgccttggtc gaggcgacgc cgatctcggg
     1621 gccggcccgc gtcatcagcg tgagtgccga ttcgcgcacg atcgacgatt ccggcacgtt
     1681 gcacaccgcg agcgtcgcca ggtaattctt cgatttcgcc agcttcaacg cggccagggt
     1741 gtccgcggtt tcgccggatt gactgatggt gacgaacagc gtgtcggcgg gcaccacggc
     1801 atcgcgatag cggtattcgg aggcgatctc cacattgcac ggaatgccgg cgagggcctc
     1861 gatccagtag cgcgcgacga gggcggcgtg ataactcgaa ccgcaggcaa tcaggtgcac
     1921 gcaacgggtt ttcgtcagca gttcgcgcgc gccaacgccg aagatttcgg tgaggatgtg
     1981 gccagcgctg atgcggcctt cgagcgtgtc cgccaatgcc tgcggctgct cgtggatttc
     2041 cttgagcatg tagtggcgat attcgccgcg ctcgaccgcg tcggcgccga aatgcgcctg
     2101 cttgatggga cgctgcacga cgtggccgtg ttcgtcgtgg atcgtgatgc catcgaccgt
     2161 cgcatcggcg atgtcgcctt cggcaaggta gatgaagcgc cgcgtcaccg gcagcagggc
     2221 gtggatgtcc gaggcgatga attgctcgcc atcggccagg cccgccacca tgggactgcc
     2281 gcggcgcgcg ccgatgacgc ggccgggttc gcgcttgctg atcaccgcga tcgcatacgc
     2341 gccgcgcagg cgcggcaggg ttttctgcac cgcggcgagc agatccgcgc cctggttctg
     2401 gtaatgctcg atcaggtgcg cgatgacttc ggtgtcggtg tcggaatcga acgcgaagcc
     2461 gagggccttg agttccgcgc gcagttcctc gtggttctcg ataatgccgt tgtgcacgac
     2521 cgcgatgttg ccgcgcgaga tcattggatg cgcatttacc gtgttcggcg cgccgtgcgt
     2581 ggcccagcgc gtgtgggcga tgccggtgaa accggtgatc gggtgcgagg tgtgcaaggt
     2641 ctcaagctcg tgtaccttgc ccttggtgcg cacgcgctcc agcccggtat cggtgagcag
     2701 ggcgatgccg gccgaatcgt agccacgata ttccagtgcc ttcaggcccg cgatcagcgt
     2761 gcccaccaca tcgcgccgcg cggcggcgcc gacaattccg cacatggatg aatcctcgaa
     2821 atccgatgcg tattttagcc gctatacggc tggatacgcg gctatttcgg ggttttttgc
     2881 gggcgtttcc agttcggaac cgtccgctgc cgcgcacgcg cgacggtgag ttcgcccggt
     2941 ggcgcgtcgg cggcgatggt cgaaccggcg ccgatggtgg cgtccttgcc gatggtcacc
     3001 ggggcgacga gtgcggaatt ggaaccgatg aatgcaccgt cctcgatgac ggtgcgg
//
LOCUS       DASRVO010000780         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_75300, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000780 DASRVO010000000
VERSION     DASRVO010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75300"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VFF05_09645"
     CDS             <1..545
                     /locus_tag="VFF05_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZW52115.1"
                     /translation="AALVLLDPPPQNVGEFSPEFEKADSAQRGEMLAAVRNCEKGAEG
                     GKLDSPPPEFKDCLRPPNPQFSTRLNAAMRANKIKPSFWQTIISIAETNADLYRQPVP
                     KSETHGAMPLLILQPDAPFEDVPPEFRKAMEQARQKTQQAIAATSTQGKIIPVAHSSH
                     DVQIDRPDAVIDAVRAVVRH"
     gene            complement(557..>1009)
                     /locus_tag="VFF05_09650"
     CDS             complement(557..>1009)
                     /locus_tag="VFF05_09650"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52116.1"
                     /translation="PLMRVFRARIEAEFATRNLDPAKHLVFLPVHNHADYLAGIARSP
                     LVLDSPWFSGGGTSLDAFSVGTPVLAWEGAMARGRQTSAMLRMLEIPELIATDEDDYV
                     AKAVALMREPARREALRETIRARQTRLFDDDAAVRAFADFLRNAPTSH"
BASE COUNT          200 a          329 c          320 g          160 t
ORIGIN      
        1 cggccgcact cgtcctgctc gatccgccac ctcagaacgt cggcgagttt tcgccggagt
       61 tcgagaaggc ggactccgcg caacgcggcg agatgctggc cgccgtgcgt aattgcgaaa
      121 agggagccga aggcggaaaa ctcgactcgc ctccgccgga attcaaggac tgcctgcgcc
      181 cgcccaatcc gcagttcagc accaggctca atgccgcgat gcgcgccaac aagatcaagc
      241 cttcgttctg gcagacgatt atctcgatcg cggaaaccaa cgccgatctg tacaggcagc
      301 cggtgccgaa atcggaaacg catggcgcga tgccgctgct gatcctgcaa cccgatgcgc
      361 cgttcgaaga cgtgccgccg gaattccgca aggccatgga gcaggctcgg cagaagacgc
      421 agcaggccat agcggcgact tccacgcagg gcaagatcat tcccgtcgcg cattcctcgc
      481 acgatgtgca aatcgaccgt ccggacgccg taatcgacgc cgtacgcgca gtcgtccggc
      541 actgagtggc gtcggactag tgagatgttg gcgcgttgcg gagaaagtcc gcgaatgcgc
      601 gcaccgcggc atcgtcgtcg aacaggcgcg tttggcgcgc gcggatcgtt tcacgcaggg
      661 cttcgcgtcg cgcaggttcg cgcatcaagg caaccgcctt ggcgacgtaa tcgtcctcgt
      721 ccgtcgcgat caattccggg atttccagca tgcgcagcat ggccgacgtc tgccggccgc
      781 gcgccatcgc gccttcccag gccagcaccg gcgtgccgac gctgaaggcg tcgaggctgg
      841 tgccgccgcc ggaaaaccac ggcgaatcaa gcaccagcgg cgaacgcgct atgccggcga
      901 gatagtcggc atggttatgt accggcagga acaccagatg tttggccgga tcgagattgc
      961 gtgtcgcgaa ctcagcttcg atgcgcgcgc gaaacacgcg catcagcgg
//
LOCUS       DASRVO010000781         1354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_50251, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000781 DASRVO010000000
VERSION     DASRVO010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50251"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VFF05_09655"
     CDS             complement(<1..362)
                     /locus_tag="VFF05_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZW52117.1"
                     /translation="MLTRRIIPCLDVRDGKVVKGVRFRAHAIVGDIVDLAARYRDQGA
                     DELVFYDIAASPERRSVDRDWVQRVARMIDIPFCVAGGIRDVEGARALLHAGADKISV
                     NSPALERPRLIDELAEAFG"
     gene            complement(356..1087)
                     /locus_tag="VFF05_09660"
     CDS             complement(356..1087)
                     /locus_tag="VFF05_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HZW52118.1"
                     /translation="MTVQIMPAIDLRNGHVVRLRQGDYARESRYAVDALALADSHAQA
                     GARWLHMVDLDGARSGDFANLRTIEAIARAGRLAVQAGGGVRDESGVQRLFDAGVARV
                     VVGSIAVREPEVVERWIARFGAERIVIALDARHDGGVWKLPSAGWTQDEAATLDDLAP
                     RFARAGARHLLCTDIARDGMLSGPNLGLYAHLRGIAPTLSLIASGGIRDIADLQALHG
                     SGVAAAVLGRSLLDGVMGLGEALAC"
     gene            complement(1084..>1354)
                     /gene="hisH"
                     /locus_tag="VFF05_09665"
     CDS             complement(1084..>1354)
                     /gene="hisH"
                     /locus_tag="VFF05_09665"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513004.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZW52119.1"
                     /translation="GATLRVPHMGWNALRIERAASLVGGIKSGDFVYFVHSYAAAVGV
                     ETLASCEYGPAFSAVVCSGNFFGMQFHPERSGEVGARLLRNFLDL"
BASE COUNT          251 a          494 c          422 g          187 t
ORIGIN      
        1 ccgaacgcct cggccaattc gtcgatgagt cgtggacgct ccaacgccgg agaattgacc
       61 gaaatcttgt ccgcgcccgc atgcagcaga gcacgcgcgc cttcgacatc gcgaatgccg
      121 cccgcgacgc agaacgggat gtcgatcatg cgggcaacgc gttgcaccca gtcacgatcc
      181 acgctgcgcc gttcggggct cgccgcgatg tcgtagaaca caagctcgtc cgcgccctgg
      241 tcgcgatacc gcgcggccag atcgacgata tcgccgacga tggcgtgcgc acggaagcgc
      301 acgcccttga cgaccttgcc gtcgcgcacg tccaggcacg ggatgattcg acgcgtcagc
      361 acgccagcgc ctcgcccagg cccatcacgc catcgagaag gctgcggccc agcacggctg
      421 ccgcgacgcc gctgccgtgc aatgcctgca gatcggcgat atcgcgtatg ccaccggagg
      481 cgatcagcga cagcgtgggc gcgatcccgc gcagatgcgc atacagcccc agattcggac
      541 ccgacaacat gccatcgcgt gcgatatcgg tgcacagcaa gtggcgtgcg ccggcgcgcg
      601 caaagcgcgg cgccagatcg tcgagcgtcg ccgcctcatc ctgcgtccag ccggcgctcg
      661 gcagcttcca cacgccaccg tcatgacgcg catcgagcgc gatgactatg cgctcggcac
      721 cgaaccgcgc aatccagcgt tccacgactt cgggctcgcg caccgcgatg ctgccgacga
      781 cgacacgcgc cacgccggca tcgaacaggc gctgcacgcc ggattcgtcg cgcacgccgc
      841 ctccggcctg caccgccagc cgtccggccc tggcgatcgc ctcgatcgtg cgcaggttgg
      901 cgaaatcgcc ggagcgggcg ccatcgagat cgaccatgtg cagccagcgc gcccccgcct
      961 gcgcgtggct gtccgcgagc gcgagggcgt cgacggcgta gcgggattcc cgcgcatagt
     1021 cgccctgacg caggcgcacg acgtgtccat tgcgcaaatc gatggcaggc atgatctgca
     1081 cggtcagaga tccaggaagt tccgcagcaa tctcgcaccg acctcgccgg agcgttcggg
     1141 atgaaactgc atcccgaaaa aattcccgct gcacacgacc gcggagaacg ccgggccgta
     1201 ctcgcatgac gccagcgttt cgacgccaac cgcagcggca tagctgtgca cgaagtacac
     1261 gaagtcgccc gacttgatgc ctccgaccag ggaagctgca cgctcgatgc gcagtgcatt
     1321 ccagcccatg tgcggcacgc gcagcgtcgc gcca
//
LOCUS       DASRVO010000782         1093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_360134, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000782 DASRVO010000000
VERSION     DASRVO010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360134"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1093)
                     /locus_tag="VFF05_09670"
     CDS             complement(<1..>1093)
                     /locus_tag="VFF05_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HZW52120.1"
                     /translation="AAMTLDAFAGRSSAFKPEVHALRPHPGQVETAANLRRLLHGSTL
                     ADIPYHLVPRFKTWLADSWSDPSAQAYRFDIGWDWVPPSQRHGKEAFYARFLPFKGGK
                     KHQPQDAYCLRCIPQVHGAVRDALAQAQRVIDIELNAVTDNPLIFPDAPDARVIEDQV
                     ISAGHFHGMPLALAMSYVKTAIPVLASISERRLNKLVDPANNDGLPAFLIGNEDATDS
                     GFMIVQYTAAALVNDLATRAHPACVYSIPTSANAEDHVSMGANEARHVLDMLDDLGHV
                     LALELYTAAQALEYRQDMLNAARALAARGDWQALARKVAGAAGAERQPFEGEVKALMH
                     ALERAGEFRAGDAVRAAFAKIRERVAFISR"
BASE COUNT          183 a          359 c          367 g          184 t
ORIGIN      
        1 gcggctgatg aaggccacgc gctcgcggat tttggcgaaa gccgcgcgca ccgcgtcgcc
       61 ggcgcggaac tcgccggcgc gttccagcgc atgcatcaat gccttgacct cgccttcgaa
      121 cggctggcgt tcggcgccgg cagcgccggc aaccttgcgt gccaacgcct gccaatcgcc
      181 acgcgctgcc aatgcgcgcg cggcattgag catgtcctgg cggtattcca gcgcctgcgc
      241 ggcggtgtac agctcgagcg ccagcacgtg gccgagatcg tcgagcatgt cgagtacatg
      301 gcgcgcctcg ttcgccccca tcgagacatg atcctcggcg ttcgcgctgg tcgggatcga
      361 atacacgcag gccgggtgcg cgcgcgtggc gaggtcgttg accagcgcgg cggcggtgta
      421 ctgcacgatc atgaacccgg aatcggtggc gtcctcgttg ccgatcagaa atgccggcag
      481 gccgtcattg ttggccggat cgacgagctt gttcaagcgc cgttcgctga tcgatgccag
      541 cacaggaatt gccgtcttga cgtaactcat cgccagcgcc agcggcatgc cgtggaagtg
      601 tccggccgaa atcacctgat cttcaatcac cctcgcgtcc ggcgcgtcgg gaaagatcag
      661 cggattgtcg gtgacggcgt tcaactcgat gtcgatcacg cgttgcgcct gcgccagcgc
      721 gtcacgcacc gcaccatgca cctgcgggat gcagcgcagg cagtacgcat cctgcggctg
      781 gtgcttcttg ccgcccttga acggcaggaa gcgcgcataa aacgcctcct tgccgtggcg
      841 ttgcgacggc ggcacccagt cccagccgat gtcgaaacga tacgcctggg cggatggatc
      901 gctccacgaa tccgcgagcc aggtcttgaa gcgcggcacg agatggtagg gaatgtccgc
      961 cagcgtcgaa ccgtgcagaa ggcggcgcag gttcgcggcc gtttcgacct gccccggatg
     1021 cgggcgcagc gcgtgcactt cgggcttgaa cgcggacgag cgcccggcga aagcgtcgag
     1081 cgtcatcgcg gcg
//
LOCUS       DASRVO010000783         2350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_177854, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000783 DASRVO010000000
VERSION     DASRVO010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177854"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..>2350
                     /locus_tag="VFF05_09675"
     CDS             65..>2350
                     /locus_tag="VFF05_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZW52121.1"
                     /translation="MNLASAFRFSSSLVAVLDAGDGRIVDVNPAFERELGYRRTAIIG
                     HSPMEIAFWRDLQTRAAIWAHLRREHRVCGERVVFRNHAGVEYDADLFAEFFEHDGRR
                     LVLAVLQRFAPVGERVASADPDPGSYRSLFMSSAEGLYRSLPEGGLIDVNPALARIFG
                     YESPAQMLSEAHDRRARDLYADPAVAASLIERLHADGHFENQRAQIRRRDGTVVWISE
                     NSRCVRDAEGHLLFYEGSIMDIGAQVEAEERLRQSENMYRTLVDSSHDGVFLIRHDGT
                     IGFINEAMAQTLGYRAEELTGTSYLKLIAPEALAEQMVRRNERAAGSYAVQTYDTVMI
                     RKDGTRRLLHVHAGAVDYEGEVASTGTARDVTEEHRQRQALEWAERTYRELFQNAVSG
                     MFRSQLDGRIVAANDAFAHILGYANAAELLESGRGIGDFYANPDSRKYVLAQLAASRE
                     PVMFEFDARRKDGSLVPTEVRAQAIRDSEGRILWTEGSAQDIGARRRAEVALKESEAR
                     YRTLVEHSQVGVYMMLDDCYTYVNAAFAALFGYREDELIGADFRLLVPPESRQHQEDR
                     YRRRREGEPSGGDYCIVLMRKDGQRIQTVVSAGHVEMNGKRYTSGTIRDVTAQLRVQS
                     ELEHNASHDLLTGLPNRLFFERQLEQALAHAQASGDYHYAVLFLDLDGFKLVNDSLGH
                     ASGDDLLVQIAETLRANLAGHCLVARYGGDEFTLLPHGACPRGRAEQLAQRVLALLAG
                     SFEVRGHRVFSGASVGVVLGRA"
BASE COUNT          409 a          765 c          759 g          417 t
ORIGIN      
        1 tgcatgcgca tttgccgcga tatactcaac cgagaaaata tacggtgccg ttggaacggg
       61 ggacatgaat ctcgccagtg cgttccgttt ctcgtccagc ctcgtcgccg ttctggacgc
      121 cggggacggg cgcattgtgg acgtgaatcc cgccttcgag cgcgagttgg gctatcgccg
      181 cacggcgatc atcgggcaca gcccgatgga gattgcgttt tggcgcgatt tgcagacccg
      241 tgcggcgatc tgggcgcacc tgcgccgcga acaccgcgtc tgcggcgagc gcgtcgtgtt
      301 ccgcaaccat gccggcgtcg aatacgacgc ggatctgttc gccgaatttt tcgagcacga
      361 cggccggcgc ctggtcctcg ccgtgctgca gcgttttgcg ccggtgggcg agcgtgtggc
      421 ctccgcggat cccgatcccg gcagttaccg ctccttgttc atgtccagtg cggaaggttt
      481 gtaccgcagc ctgcccgagg gcgggctgat cgacgtgaac cccgccttgg cgcgcatttt
      541 cggctatgaa tcgccggcgc aaatgctgag cgaagcccat gatcggcgtg cccgcgatct
      601 ttacgcggat ccggcggtcg ccgcatccct gatcgaacgc ctgcacgcgg acgggcattt
      661 cgagaatcag cgtgcgcaga tccgccgccg cgatggcacc gtggtgtgga tttcggaaaa
      721 ttcgcgctgc gtgcgcgacg ccgaggggca cctgctgttc tacgaaggct cgatcatgga
      781 catcggtgcg caggtcgagg cggaggagcg cctgcgtcag tccgagaaca tgtaccgcac
      841 cctcgtcgac agcagccacg acggcgtttt cctgatccgg cacgacggca ccatcggttt
      901 catcaacgaa gcgatggcgc agaccctggg ataccgcgcc gaggaactga ccggcaccag
      961 ttacctcaaa ctcatcgcgc ccgaggccct ggccgaacag atggtccgcc gcaacgaacg
     1021 cgcggccggt tcgtatgcgg tgcaaaccta cgacacggtg atgatccgca aggacggcac
     1081 gcgccgcctc ctacacgtgc atgccggtgc ggtcgattac gagggcgaag tggccagcac
     1141 cggtaccgcg cgcgacgtca ccgaggaaca tcgccagcgc caggcgctgg aatgggccga
     1201 acgcacctac cgcgagctgt tccagaacgc ggtgtcgggc atgttccgct cgcaactcga
     1261 cggtcgcatc gtcgctgcca acgacgcgtt cgcgcacatt ctcggctatg ccaatgccgc
     1321 cgaactgctc gaatccggac gcggcatcgg cgatttctac gcgaatcccg acagtcgcaa
     1381 gtacgtgctg gcgcaactcg ctgcgagtcg ggaaccggtg atgttcgaat tcgacgcccg
     1441 tcgcaaggac ggcagtctcg tgcctaccga ggttcgcgcg caggcgatcc gcgacagcga
     1501 gggtaggatt ctctggaccg aaggttcggc ccaggacatc ggtgcgcgtc gccgcgccga
     1561 agtcgcgctg aaggaatccg aggcgcgcta ccgcaccctg gtcgagcaca gtcaggtcgg
     1621 tgtgtacatg atgctcgacg actgctacac ctacgtcaac gcggctttcg ccgccctgtt
     1681 cggatatcgc gaggacgaac tgatcggcgc cgattttcgc ctgctcgtgc cgcccgaatc
     1741 gcgccagcat caggaagacc gttatcgtcg ccgccgtgaa ggcgagccga gcggtggtga
     1801 ttactgcatc gtgctgatgc gcaaggacgg ccagcgtatc cagaccgtcg tcagcgccgg
     1861 ccatgtcgaa atgaatggca agcgctacac cagcggcacg atccgtgacg tgaccgcgca
     1921 attgcgtgtg cagagcgaac tggaacacaa cgcgtcgcac gatctgctga ccggtctgcc
     1981 caatcgcctg ttcttcgagc gccagctcga acaagcgctc gcccacgcgc aagccagtgg
     2041 cgattaccac tacgcggtgc tgttcctcga tctcgacggc ttcaagctgg tcaacgacag
     2101 tcttggccac gccagcggcg acgacctgct ggttcagatc gccgaaacct tgcgcgcgaa
     2161 tctggccggg cattgtctgg tggcgcgcta cggcggcgac gagttcaccc tgctgcctca
     2221 cggcgcctgc ccacgcggcc gcgccgaaca actggcgcaa cgcgtgctcg cgctgctggc
     2281 gggttcgttc gaggtgcgtg gtcaccgggt gttttccgga gccagcgtcg gcgtggtgct
     2341 gggccgcgcc
//
LOCUS       DASRVO010000784         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_360182, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000784 DASRVO010000000
VERSION     DASRVO010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360182"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFF05_09680"
     CDS             complement(<1..295)
                     /locus_tag="VFF05_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52122.1"
                     /translation="MNALSSTVRHLAETPRQPPWKVLVVDDEPEVHAVTRLVLGSFRF
                     AERPLQFLNANSAAQAEAMLREHPDIAVMLLDVVMETDKAGLDLVRTVRERHGN"
     gene            complement(417..1574)
                     /locus_tag="VFF05_09685"
     CDS             complement(417..1574)
                     /locus_tag="VFF05_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HZW52123.1"
                     /translation="MRSFPLGEEIDLLRESVHAFAEKEIAPRADLIDRENAFPHDLWR
                     KLGDMGLLGVTVSSEYGGSGMGFLAHLVAMEEISRASGSVGLSYGAHSNLCVSNIFHN
                     GSQAQRQKYIPRLCSGEWVGALAMSEPGAGSDVVGSMTCKAERKGDAWIANGNKMWIT
                     NGPDADVLLVYMRTADKRAGSRCMTAFIVEKGMPGFSTAQKLDKLGMRGSNTCELVFE
                     NCEIPDANRVGEVNEGVRVLMSGLDTERLVLSGGPVGLMQAALDITLPYVRERRQFNA
                     PIGTFGIMQAKIADMYTLLQSARAYAYMVARDFDHGVKSRVDPASCLLHASRSAVQMA
                     LEAIQALGGNGYINEYPAGRILRDAKLYEIGAGTNEIRRMLIGRELFHGKW"
     gene            complement(1802..>2839)
                     /locus_tag="VFF05_09690"
     CDS             complement(1802..>2839)
                     /locus_tag="VFF05_09690"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZW52124.1"
                     /translation="GLKAIIAIHNTVLGPALGGLRMWPYKSEQEALNDVLRLSRGMTF
                     KNAVAGLNIGGGKAVIIGDPAKDKSEALFRAFGRFVNSLNGRYITAEDVGIDVNDMEW
                     VFRETEFVTGVHQVHGGSGDPSPFTAFGTLQGLMAALQAKFGDEDVGKRAYAVQGAGH
                     VGMEFIKLLREQGAKVFVTDINPELVKRAVDEFGCTAVGLDEIYDVDADVYSPCALGG
                     TVNEKTLPRIKAKIICGAANNQLANDAIGDELEKRGVLYAPDYAVNAGGVMNVSLEID
                     GYNRERAMRMMRTIYHNVGRIFQIAKRDGIPTYKAADRMAEERIAAIGKIKLPHMGNN
                     GPRFLGRMRGH"
BASE COUNT          464 a          906 c          919 g          550 t
ORIGIN      
        1 ggttgccatg gcgctcgcgc acggtgcgca ccaggtcgag gccggccttg tcggtttcca
       61 tgaccacgtc gagcagcatc acggcgatgt ccggatgctc gcgcagcatc gcctcggcct
      121 gggccgcgga gtttgcgttg aggaattgca ggggacgctc ggcgaaacgg aagctgccca
      181 acaccaggcg cgttaccgcg tggacttcgg gttcatcgtc gacgaccagc actttccatg
      241 gcggttggcg cggggtctcc gccagatgcc gcacggtgct gctcagagcg ttcatgatcc
      301 acccccgttc cgtggatgcg tcgagtgtag cgcagatgac acggctcttg gcaatccttg
      361 tccggacagg cggcaggatc ggcagtcagg cggtggtgcg gcgatctaat tcgtcgctac
      421 cacttgccgt ggaacaactc gcggccgatc agcatgcgcc ggatttcgtt ggtgccggcg
      481 ccgatttcgt agagcttggc gtcgcgcaaa atgcgcccgg ccggatactc gttgatgtag
      541 ccgttgccgc cgagcgcctg gatcgcttcc agggccatct gcaccgcgct tcgcgaggca
      601 tgcagcaggc acgaggccgg atcgacgcgc gacttcacgc catggtcgaa atcccgcgcg
      661 accatgtacg cataggcgcg cgcggattgc agcaatgtat acatgtccgc aatcttggcc
      721 tgcatgatgc cgaaggtgcc gatcggcgcg ttgaactggc ggcgctcgcg tacgtagggc
      781 agggtgatgt cgagcgccgc ctgcatcagg ccgaccgggc cgccggagag caccaggcgc
      841 tcggtgtcca gcccgctcat cagcacgcgc acgccttcgt tgacttcgcc gacgcggttg
      901 gcgtccggga tctcgcagtt ctcgaagacg agttcacagg tattgctgcc gcgcatgccg
      961 agcttgtcga gcttctgcgc ggtggaaaag cctggcatgc ctttttcgac gatgaacgcg
     1021 gtcatgcagc ggctgccggc gcgcttgtcg gcagtacgca tgtagaccag cagcacgtcg
     1081 gcatccggac cgttcgtgat ccacatcttg ttgccgttgg cgatccaggc gtcgcccttg
     1141 cgttcggcct tgcaggtcat cgaaccgacc acatcggagc cggcgcccgg ctcgctcatc
     1201 gccagcgcgc cgacccactc gccggaacac agtctgggaa tgtacttctg gcgctgcgct
     1261 tggctgccgt tgtggaaaat gttgctgacg cacaggttcg aatgcgcgcc gtaggacaag
     1321 cccaccgaac cggatgcgcg cgagatttcc tccatcgcca cgaggtgggc gaggaaaccc
     1381 atgcccgagc cgccgtattc gctcgacacg gtgacgccga gcagacccat gtcgccgagc
     1441 ttgcgccaca ggtcgtgggg gaacgcgttc tcgcggtcga tcaggtccgc gcgcggcgcg
     1501 atttccttct ccgcgaaagc atgcacgctc tcgcgcagca ggtcgatttc ttcgccgagg
     1561 gggaaggatc gcatcgcaaa tctcaccttg gttttccgca atgatacaag cagcagccac
     1621 gctacgtcgt aacccggctg ccgctacgaa tcacgagcgc cgaatatgag tggaaccttc
     1681 atcccatggc aaattggcgt gcgtaccctc cggagggccg cgccctgtac gcgtttttgg
     1741 ttgctttttg cgcgcgcaaa aagtaacccg ggcggggcag ccgcacggaa gccttgctct
     1801 gtcagtggcc tcgcatgcga cccaggaacc gtggcccatt gttgcccatg tgcggcaact
     1861 tgatcttgcc gatcgcggca atgcgctcct cggccatgcg gtcggccgcc ttgtaggtcg
     1921 ggatgccgtc gcgcttggcg atctggaaga tgcggccgac gttgtgatag atcgtgcgca
     1981 tcatgcgcat ggcgcgttcg cggttgtagc cgtcgatttc cagcgagacg ttcatcacgc
     2041 caccggcatt caccgcgtag tccggggcat acaggacgcc gcgcttctcg agttcgtcgc
     2101 cgatcgcgtc gttggcgagc tggttgttgg cggcgccgca gatgatcttg gccttgatgc
     2161 gcggcagcgt cttttcgttg accgtgccgc cgagcgcgca cggcgagtag acgtcggcgt
     2221 cgacgtcgta gatttcgtcc aggccgaccg cggtgcagcc gaattcgtcg accgcacgct
     2281 tgaccagttc cggattgatg tcggtgacga acactttcgc gccttgttcg cgcagcagct
     2341 tgatgaactc catgccgaca tggccggcgc cctgcacggc ataggcgcgc ttgccgacgt
     2401 cctcgtcgcc gaacttggct tgcagcgcgg ccatcaagcc ctgcaacgtg ccgaacgcgg
     2461 tgaacggcga gggatcgccc gagccgccat gcacctggtg cacgccggtg acgaattcgg
     2521 tttcgcggaa cacccattcc atgtcgttga cgtcgatgcc gacgtcctcg gcggtgatgt
     2581 agcggccgtt gagcgagttg acgaagcggc cgaacgcgcg gaacagcgcc tcggacttgt
     2641 ccttggccgg atcgccgatg atcaccgcct tgccgccgcc gatgttgagg ccggccaccg
     2701 cgttcttgaa cgtcatgcca cgcgacaggc gcaacacgtc gttcagggct tcctgctcgc
     2761 tcttgtacgg ccacatgcgc aggccgccga gcgccggacc gagtaccgta ttgtggatgg
     2821 cgatgatggc cttgaggcc
//
LOCUS       DASRVO010000785         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_75493, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000785 DASRVO010000000
VERSION     DASRVO010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75493"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFF05_09695"
     CDS             complement(<1..1334)
                     /locus_tag="VFF05_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HZW52125.1"
                     /translation="MTFLLMLAGAALGAFFCAGMFDEPTGWTAAMLGGCIGLLLGQLR
                     SVRLRLAAIEKNLGDLRVEAALQGARATQAAREIPAPTPQPISEPERAPVPPAPAIAE
                     YRAPALVIPERVAPAPQIPAPPAPPDFIARSIDRVKRWFTEGNVPVKVGIVVSFFGVG
                     ALLKYAADSGWLVVPIEMRLLGIAAAALVALVFAWRKRDSHRAFALSLQGGAIGVLIL
                     TIFGAYRIWALLPSALTFALLIVVVAGAGLLAVLQDALALAVLAIVGGFLAPILSSSG
                     HGSHVALFGYYAVLNAAIFAIAWIKPWRALNLLGFAFTFGIGAAWGVQQYRPEFFAST
                     EPFLALFFAFYLFVPLAYALRLAPHKRDLIDGTLVFGTPLLAMPLQAALLDYARMSMA
                     WSALALAAVYTPLAWLELRRLRLQLLGESHALLALGFATLAIPLAFSARSTA"
     gene            complement(1415..>1729)
                     /locus_tag="VFF05_09700"
     CDS             complement(1415..>1729)
                     /locus_tag="VFF05_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971665.1"
                     /note="Acylates the intermediate (KDO)2-lipid IVA to form
                     (KDO)2-(lauroyl)-lipid IVA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HZW52126.1"
                     /translation="QDMRGKDTVFAPFFGVNASTLTATHHLTRLSGAAVIPFFHKRES
                     HGGYAIRLEAPLADFPSADVTADTARVNACIERMVHEAPAQYLWVHKRFKTRPPGEPS
                     PY"
BASE COUNT          335 a          546 c          610 g          238 t
ORIGIN      
        1 caggccgtgg agcgcgccga gaacgccagc ggaatcgcca gcgtggcgaa gccgagcgcg
       61 agcagcgcat gcgattcgcc gagtagctgc aagcgcaggc gtcgcaattc tagccaggcg
      121 agcggcgtgt ataccgccgc cagcgcaagc gcgctccacg ccatcgacat gcgtgcgtag
      181 tccagcaatg ccgcctgcag cggcatcgcg agcagtggcg tgccgaacac gagcgtgcca
      241 tcgatcagat cgcgtttgtg cggcgccagc cgcagcgcgt aggcgagcgg cacgaacaga
      301 tagaacgcga agaacagcgc gagaaaaggt tcggtggacg caaaaaattc cggccggtac
      361 tgctgcacgc cccaggcggc gccgatcccg aacgtgaagg cgaaaccgag caggttcagc
      421 gcgcgccacg gcttgatcca ggcgatcgcg aagatcgccg cattgagcac ggcgtaatag
      481 ccgaacaagg cgacatggct gccgtgtccg ctcgatgaca ggatcggggc gagaaagccg
      541 ccgactatcg cgagcacggc gagcgcgagg gcatcctgca gcaccgcgag caagccggcg
      601 ccggcgacca caacaatgag caacgcgaag gtcagcgccg aaggcagcaa ggcccagatg
      661 cgatacgcgc cgaagatggt caggatcagc acgccgatgg cgccaccctg caggctcagc
      721 gcgaacgcgc ggtgcgaatc gcgcttgcgc cacgcgaaca cgagcgccac gagcgcggcg
      781 gcggcgatgc cgagcaggcg catttcgata ggcacgacca gccagccgga gtccgcggca
      841 tatttgagca aggcgccgac gccgaagaac gatacgacga tgccgacctt gaccggcacg
      901 ttgccttcgg tgaaccagcg tttcacgcga tcgatcgacc gcgcgatgaa gtccggtggc
      961 gcgggcggcg ccggaatttg cggcgcgggt gcgacgcgtt ccgggatgac cagtgccggc
     1021 gcacggtatt ccgcgattgc cggcgcgggt ggcacgggcg cgcgctcggg ttcggagatg
     1081 ggttgtggcg ttggcgcggg aatctcgcgt gcggcttgcg ttgcacgcgc gccttgcagt
     1141 gcggcctcga cacgcagatc gcccagattc ttctcgatgg cggcaagacg caggcgtacg
     1201 ctgcgcagtt gtcccagcaa caggccgatg cagccgccca gcattgccgc cgtccaaccg
     1261 gtgggttcgt cgaacatgcc ggcgcaaaag aaggcgccga gcgcggcgcc ggccagcata
     1321 agcaggaaag tcatgtgcga tccccatcag cacgcgagcg gcggcacctt agcatgatcc
     1381 gtggaaagcg gtgcaaggtc gtggtttgcc ggcgtcaata cggcgatggt tctcccggcg
     1441 gccgcgtctt gaagcgcttg tgcacccaca ggtattgcgc gggcgcctcg tgcaccatgc
     1501 gctcgatgca ggcattcacg cgcgcggtgt ccgcggtgac gtcggcggag gggaaatctg
     1561 ccagcggcgc ttccagccgg atcgcatagc cgccgtgtga ttcgcgcttg tggaaaaacg
     1621 ggatcaccgc tgcgcccgac agccgcgtca ggtggtgggt ggccgtgagc gtggacgcgt
     1681 tcacgccgaa gaacggcgcg aacactgtat ccttgccgcg catgtcctg
//
LOCUS       DASRVO010000786         1015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_75508, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000786 DASRVO010000000
VERSION     DASRVO010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75508"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFF05_09705"
     CDS             <1..507
                     /locus_tag="VFF05_09705"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZW52127.1"
                     /translation="RAQPDFPPIIMLSAKGEDIDRIIGLEVGADDYLPKPFNPRELLA
                     RIRAVLRRQNGAAAPAVPETARLAHFGPFSVNLDARTLSRDDAEIPLTSGEYELLEIF
                     VTHANRALSRDWLMDQLRGFERDPFDRSIDVRVNRLRKKIEDDPANPAYIRTQRGQGY
                     LFVPQGKG"
     gene            504..>1015
                     /locus_tag="VFF05_09710"
     CDS             504..>1015
                     /locus_tag="VFF05_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52128.1"
                     /translation="MRHLPRSLFARTALTLALAFIVFQAAAFWVVYRTLIVPVAERSA
                     DDLAGLIVLSAQTWVELPPETRAAFERELARRHGLRLTIVDIGATADAPHFAFRTQIE
                     AALSRRVGDDVALRGVPGTAAAWLDIPVGGHNLHVGFFPRRYAVKPPLAAIAVVALGT
                     FLSLLTALFLV"
BASE COUNT          149 a          336 c          336 g          194 t
ORIGIN      
        1 cgtgcgcagc cggactttcc gcccatcatc atgctgtcgg ccaaaggcga ggatatcgac
       61 cgcatcatcg gcctcgaggt cggcgccgac gattacctgc ccaagccgtt caatccgcgc
      121 gaactgctgg cccgtatccg cgcggtgttg cggcggcaga atggcgcggc agcaccggcc
      181 gtgcccgaaa ccgcccggct ggcgcatttc ggcccgttca gcgtcaacct cgacgcccgt
      241 acgctcagtc gcgacgatgc cgagatcccg ctgaccagcg gcgaatacga actgctggaa
      301 atcttcgtta cccatgccaa ccgtgccctg tcgcgcgact ggctgatgga tcagctgcgt
      361 ggtttcgaac gcgacccgtt cgaccgcagc atcgacgtgc gggtgaatcg tctgcgcaag
      421 aaaatcgagg acgacccggc caatccggcc tatatccgca cccagcgcgg acagggctat
      481 ctgtttgtgc cgcagggcaa agggtgagac atcttccccg ctcgttgttc gcccgcacgg
      541 cgctgacgct ggcgctggcg tttatcgtgt ttcaggcggc agcgttctgg gtggtgtatc
      601 gcacgctgat cgtgccggtg gccgagcgct cggccgacga tctggccggc ctgatcgtgc
      661 tgtcggcgca aacctgggtg gagctgccgc cggagacgcg cgcagcgttc gagcgtgaac
      721 tggcgcgccg tcatgggctg cgcctgacca ttgtggacat cggcgccacg gcggatgcgc
      781 cgcatttcgc ctttcgtacc cagatcgagg ccgcactgag ccgtcgcgtg ggcgacgatg
      841 tggccttgcg cggcgtgccg gggacggcag cggcctggct ggacattccg gtcggcggcc
      901 ataacctgca cgtcggcttc ttcccgcgtc gctatgccgt caagccgccg ctggcggcga
      961 tcgcggttgt cgcgctcggc acgtttctga gtttgctgac tgctttgttc ctggt
//
LOCUS       DASRVO010000787         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_177928, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000787 DASRVO010000000
VERSION     DASRVO010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177928"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..952)
                     /locus_tag="VFF05_09715"
     CDS             complement(176..952)
                     /locus_tag="VFF05_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52129.1"
                     /translation="MNLGLLDLPAPLLDALDGALDRIGAPALLRVVAYAAASAWMGMA
                     LYRRLSDQRRLAEVGAQVALSQQALARHDGDFATLRVLIVANLRATSHHIALTLRPAL
                     IASLPLLFALPWLSNRFDFRQPQTGTSISVCVQPVVRGLHWQASSAAAATASGCWLTP
                     WPDVTTPATLSDSGGQTLLVMPNAAKSDTVEKFGWFNWLIGNPAGYLPADAVIARVHI
                     ALPQRQILPIGPAWLRGWVFWYLLAALLVSLWLKWRWRLH"
     gene            complement(949..>1995)
                     /locus_tag="VFF05_09720"
     CDS             complement(949..>1995)
                     /locus_tag="VFF05_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZW52130.1"
                     /translation="LEGFPRPLLYISPINFDPSDPAAAISSPPAYAAELAKRIGDYYT
                     LGMPEETWSLNQGHMSEQGWLDSVKSTLADGEKMLDDALARNDSELVIEVFVQTDRVS
                     HMFWRGIDPKHPLYAQSSALARGAIPWIYRQADRVLGDVQAKMHAGDRLIVLSDHGFA
                     SFRRAVHLNRWLADQGYLVLNPGATVSAPLFAQVDWSKTRAYALGLNGIFINEKGREA
                     LGIVATSAIAPLKAEIAAKLSGLHDGGIAVVDAVYDADAIYHGAHVGDAPDLVVGYAP
                     GYRASWQTTLGGAPAVLIEDNLQPWSGDHCIDPAAVPGVLFTSFQPDKPVASIADVAQ
                     LILRHLPAPAKSTP"
BASE COUNT          357 a          633 c          675 g          330 t
ORIGIN      
        1 aggcgccgaa cagccagccc gagccgatga tcgaacccag tcccatcagc atcagcgcaa
       61 acgcgccgac ttcgcggcgg atcgccgtgt cgtttccagt catgtgaatc ccccaagtgg
      121 cgcggcggtg cgcaatggtc gcaggtttcg cggcgggcgt cagcggtggc aggattcagt
      181 gcaatcgcca acgccatttc aaccacagcg agacgagcag cgcggcgagc aaataccaga
      241 agacccagcc gcgcaaccac gctggcccga tggggaggat ctgccgttgc ggcagggcga
      301 tgtgcacgcg cgcgatgact gcatcggccg gcaggtaacc ggccggattg ccgatgagcc
      361 agttgaacca gccgaatttc tcgacggtat cgctctttgc ggcattgggc atcacgagca
      421 acgtttgtcc gccagagtcg gacagtgttg ccggcgttgt gacatcgggc cagggtgtga
      481 gccagcaccc cgatgcggtg gctgccgcgg cgctcgacgc ttgccagtgc aggccgcgca
      541 ccacaggctg cacgcacacg gaaatcgatg tcccggtctg cggctgccgg aaatcgaaac
      601 gattggaaag ccacggcagc gcgaacagca ggggcaggct ggcgatcagc gcgggccgca
      661 atgtcagggc gatgtggtgc gaggtggcgc gcaggttggc cacgatcaag acccgcagcg
      721 ttgcgaagtc gccgtcgtgc cgcgccagcg cctgctgact gagcgcgacc tgggctccga
      781 cttcggccag gcgtcgttga tccgacaaac gccggtaaag cgccattccc atccaggcgg
      841 aagcagcggc gtatgccacc acgcgcaaca acgcgggcgc gccgattcgg tcgagtgcgc
      901 catccagcgc gtccaacagc ggcgcaggca agtcgagcag tcccaggttc acggcgtcga
      961 tttcgccggt gccggcaggt gccggaggat cagttgggcg acatccgcaa tgctcgcgac
     1021 gggtttgtcc ggttggaacg aggtgaacag cacgccgggc acggcagccg ggtcgatgca
     1081 gtgatcgccg ctccagggct gcaggttgtc ctcgatcagt acggccggtg cgcctcccag
     1141 cgtggtctgc cacgacgcac gatagccggg agcgtagccg acgaccagat ccggcgcgtc
     1201 gccgacgtga gcaccgtgat agatggcatc cgcatcgtaa accgcatcga cgacggcgat
     1261 tcctccgtcg tggaggccgc tcagcttcgc cgcgatctcc gccttgagcg gcgcgatggc
     1321 gcttgttgcg acgatgccca gcgcttcgcg gcccttttcg ttgatgaaga tgccattgag
     1381 ccccaatgcg taggcgcgcg tcttcgacca gtcgacctgc gcgaacagcg gtgcggaaac
     1441 cgtcgcgccg ggattcagga ccagataacc ctggtcggcc agccagcgat tcaggtgcac
     1501 cgcgcggcgg aacgaggcga acccgtgatc cgacagcacg atgaggcgat cgcccgcgtg
     1561 catcttcgcc tgcacgtcgc cgagcacgcg gtcggcttgc cggtaaatcc atggaatcgc
     1621 gccgcgcgcg agcgcgctgc tttgcgcgta caaggggtgt ttcgggtcga tgccgcgcca
     1681 gaacatgtgg ctgacccggt cggtctgaac gaatacctcg atcacgagtt cgctgtcgtt
     1741 tcgcgcgaga gcgtcgtcga gcatcttctc gccgtcggcg agcgtgctct tgacgctgtc
     1801 cagccagcct tgttcgctca tgtgcccctg gttcaacgac caggtttcct cgggcatgcc
     1861 cagcgtgtag taatcgccga tgcgcttggc cagttccgcg gcataggcgg gtggcgagct
     1921 tatcgccgcg gcgggatcgc ttgggtcgaa attgatcggg gaaatgtaca gcagcgggcg
     1981 cggaaagcct tccag
//
LOCUS       DASRVO010000788         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_284265, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000788 DASRVO010000000
VERSION     DASRVO010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284265"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VFF05_09725"
     CDS             <1..822
                     /locus_tag="VFF05_09725"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019237600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HZW52131.1"
                     /translation="RAIGLGACDIKGAAAGLLAAAQASDGDAAFLFTTDEEANDARCI
                     ASYLAQKMHYDAVIVAEPTRCEAVLAHRGISSVRMCFAGRAGHAAGEQTVGDSALHQA
                     VRWSGRALDHVDSLAPARFGGLTGLRFNIGRIDGGIKANMIAPSAELRFGFRPLPSMD
                     VDGLLATFRGFAEIEPAEFSETFRGPALPAGDIARAEAHRLLARDVADALDIPVGNAV
                     DFWTEAALFSQAGYTALVYGPGDIAQAHSADEWVALDQLQRYAENIYRIVNHGNA"
     gene            809..2131
                     /locus_tag="VFF05_09730"
     CDS             809..2131
                     /locus_tag="VFF05_09730"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZW52132.1"
                     /translation="METHKHTRKTIVRLLSAMGSAKEIQAYLKRFSQLDAKRFAVVKV
                     GGAVLRDELADLASSLTFLQQVGLTPIVIHGAGPQLDEEMSAAGIEKQTVGGLRVTSP
                     KALGIVRRVFVQQNLKLVEALQAMDTRATAIPSGVFMAGYLDRDALGLVGKVERIDLA
                     PIEASLRGNSIPVIASLGETDEGQLLNINADVAANELVRVLQPYKIVFLTGTGGLLDG
                     AGRVIDSINLSTEYEHLMAQPWVSGGMRLKIEQIAELLNDLPLTSSVSITRPAELAKE
                     LFTHKGSGTLVRRGEKVLRFASWDGVDRVRLRTLIESSFGRKLVTDYFERTQPFRVYV
                     SENYRAAMILTMQDGFPYLDKFAVLDDAQGEGLGRAVWLVMREENPQLFWRSRHGNGI
                     NAFYHEQADGCVRQERWRAFWYGLGVDFAAIERCVVHCAQREPTLVDA"
     gene            2128..3078
                     /gene="argC"
                     /locus_tag="VFF05_09735"
     CDS             2128..3078
                     /gene="argC"
                     /locus_tag="VFF05_09735"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZW52133.1"
                     /translation="MSRQRIGIVGARGHTGLELIQLIASHPALELAFVCSRELDGEHV
                     NQHCAAYTGDLRYENPDPAGVAAKVADVVILALPNGKAAPYVQAIEAATPQALILDLS
                     ADYRFDKNWYYGLPELTREHWRGQRRVSNPGCYATAMQLSIAPLKGLLAAPPVCFGVS
                     GYSGAGTMPSDKNDPDKLRDNLMPYALTGHTHEKEAGFQLGLPVEFMPHVAPHFRGLT
                     VTANLYLNRAMKRDEAIALFNRAYDGEKLVHVVDEAPWVSRIAGKHHVEIGGFAMSAD
                     GRRVVVVATLDNLLKGAATQAMQNINFALGFDELTGIPHD"
     gene            3071..>3242
                     /locus_tag="VFF05_09740"
     CDS             3071..>3242
                     /locus_tag="VFF05_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZW52134.1"
                     /translation="MTEPLWNKSDTRIDAKIMRFLAGDDVMLDREFFLHDIVASKAHV
                     EGLANIGVVSADD"
BASE COUNT          578 a         1009 c         1073 g          582 t
ORIGIN      
        1 cgcgccatcg gcctcggcgc ctgcgacatc aagggcgccg ccgctgggct gctcgccgcc
       61 gcgcaagcca gcgacggcga tgccgccttc ctgttcacta ccgacgagga ggcgaacgac
      121 gcgcgctgca tcgcgtcgta cctcgcgcaa aaaatgcact atgacgccgt gatcgtggcc
      181 gagccgacgc ggtgtgaagc cgtgctcgcg catcgtggca tcagttcggt gcggatgtgt
      241 tttgccgggc gcgctggcca cgctgccggc gaacagacag tgggcgacag tgcgctgcac
      301 caggccgtgc gctggagcgg ccgtgcactc gatcacgtcg attcgctcgc gcctgcgcgt
      361 ttcggaggct tgaccggatt gcgtttcaac atcggccgca tcgacggcgg catcaaggcg
      421 aacatgatcg cgccgtcggc ggaactgcgt ttcggcttcc ggccgttgcc gtcgatggat
      481 gtcgatggcc tgctcgcgac cttccgcggc tttgccgaaa tcgagccggc cgagttcagc
      541 gaaacctttc gcggcccggc gttgcccgcg ggcgacatcg caagggctga ggcgcatcga
      601 ttgctggcgc gcgacgtcgc cgacgcgctc gatattcccg tcggtaatgc cgtcgatttc
      661 tggaccgagg ccgcgctgtt ctcgcaagcc ggctacaccg cgctcgtcta cggtcccggc
      721 gacatcgccc aggcacacag cgcggatgaa tgggtggcgc tcgaccagtt gcagcgctac
      781 gccgagaaca tttaccggat cgtcaatcat ggaaacgcat aagcacaccc gcaagaccat
      841 cgtgcgcctg ctctcggcga tgggcagcgc gaaggaaatc caggcctacc tgaaacgctt
      901 ctcgcagctc gacgccaagc gcttcgccgt ggtcaaggtt ggcggggcgg tgctgcgcga
      961 cgagctggcc gatctggcct cgtcgctgac tttcctgcag caggtcggat tgacgccgat
     1021 cgtgatccac ggcgcgggcc cgcagctcga cgaggagatg tccgccgcgg gcatcgagaa
     1081 acagaccgta ggtggactgc gcgtgacctc gccgaaggcg ctgggcatcg tgcgccgcgt
     1141 gttcgtgcaa cagaacctga aactggtgga agcgctgcag gcgatggaca cccgcgccac
     1201 cgcgataccg tcgggcgtct tcatggccgg ttatctcgac cgcgacgctc tgggactggt
     1261 cggcaaggtg gagcgcatcg atcttgcacc gatcgaagcc agcctgcgcg ggaattcgat
     1321 accggtgatc gccagccttg gcgaaaccga cgagggccag ttgctgaaca tcaacgccga
     1381 tgtcgccgcg aacgaattgg tgcgggtgct gcagccgtac aagatcgtgt tcctcaccgg
     1441 caccggcggc ctgctggatg gtgcgggccg ggtgatcgat tcgatcaatc tcagcaccga
     1501 atacgagcac ttgatggcgc agccgtgggt cagcggcggc atgcgcctga agatcgaaca
     1561 gatcgccgag ttgctgaacg atctgccgct cacgtcctcg gtgtcgatca cgcgcccggc
     1621 ggagctcgcc aaggaactgt tcacgcacaa gggttcggga acgctggtgc ggcgcggcga
     1681 gaaagtgctg cgcttcgcat cgtgggacgg cgtcgatcgc gtgcgcctgc gcacgctgat
     1741 cgagtcgagc ttcgggcgca aactggtgac ggattacttc gagcgcacgc agccgtttcg
     1801 ggtttacgtg agcgaaaact accgcgccgc gatgatcttg acgatgcagg acggttttcc
     1861 gtatctggac aagttcgccg tgctcgacga cgcgcagggc gagggcctgg gtcgcgcggt
     1921 gtggctggtg atgcgcgagg agaatccgca gctgttctgg cgttcgcgcc acggcaacgg
     1981 catcaatgcg ttctatcacg agcaagccga cggctgcgtc cgccaggaac gttggcgcgc
     2041 gttctggtac ggactcggcg tcgacttcgc tgccatcgag cgctgcgtcg tgcattgtgc
     2101 gcaacgcgaa ccgacgctgg tggacgcatg agcaggcaac gcatcggcat cgtcggggca
     2161 aggggtcaca ccggtttgga actgatccaa ctgatcgcgt ctcatccggc gctggaactg
     2221 gcttttgttt gctcgcgcga actcgatggt gagcacgtca accaacactg cgctgcctac
     2281 acgggtgatc tgcgctacga gaatcccgac ccggcgggag tggcggcaaa agttgccgac
     2341 gtggtgatac tggcgctgcc caacggcaag gcggcgccct acgtccaggc catcgaggca
     2401 gccacaccgc aagccttgat cctcgatctt tccgccgact atcgcttcga caagaactgg
     2461 tactacggtc tgccggaatt gacgcgcgag cattggcgcg gacaacgccg cgtcagcaat
     2521 cccggctgct acgccacggc gatgcagctt tcgatcgcac cgctcaaggg cctgctcgcc
     2581 gcgccgccgg tgtgtttcgg cgtgtccggg tattccggcg ccggcaccat gccgtcggac
     2641 aagaacgatc ccgacaagct gcgcgacaac ctgatgccgt atgcgctcac cggccatacg
     2701 cacgaaaagg aagccggttt ccagctcgga ctgccggtgg aattcatgcc gcacgtggcg
     2761 ccgcattttc gtgggctgac ggtcacggcg aacttgtacc tgaatcgtgc catgaaacgc
     2821 gacgaagcga ttgcgctgtt caatcgcgcc tacgacggcg agaaactggt gcatgtcgtc
     2881 gatgaggcgc cgtgggtcag ccgcatcgcc ggcaagcatc atgtcgagat cggtggtttc
     2941 gccatgtcgg ccgatggcag gcgcgtcgtg gtcgtggcca cgctcgataa tctgctcaag
     3001 ggtgcggcga cgcaggcaat gcagaatatc aactttgcgc tgggcttcga cgagctgacc
     3061 gggattcccc atgactgagc ctctttggaa caaatccgac acgcgcatcg acgcgaagat
     3121 catgcgtttc ctcgccggcg acgacgtgat gctggatcgc gagttcttcc tgcacgacat
     3181 cgtggcgagc aaggcgcatg tggaaggtct ggccaacatc ggcgtggtga gcgcggatga
     3241 tg
//
LOCUS       DASRVO010000789         1221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_152398, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000789 DASRVO010000000
VERSION     DASRVO010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152398"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFF05_09745"
     CDS             complement(<1..502)
                     /locus_tag="VFF05_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HZW52135.1"
                     /translation="MAIELPALPYARDALAPHISAETIDYHYGKHHQAYVTNLNNLIK
                     GTEFEAMDLVSIVKKAQGGMFNNAAQIWNHTFYWNSLSPKGGGEPSGKLGDAIKKAFG
                     GFAQFKDDFSKLAAGTFGSGWAWLVQRVDGSLGIVSTSNAATPITGADRPLLTCDVWE
                     HAYYIDY"
     gene            601..918
                     /gene="grxD"
                     /locus_tag="VFF05_09750"
     CDS             601..918
                     /gene="grxD"
                     /locus_tag="VFF05_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZW52136.1"
                     /translation="MDTIERIKQTVQSSPIVLFMKGTPQFPMCGFSSRTAQALKAVGA
                     EYASVNVLEDPEVRANLPHYSNWPTFPQLFINGELIGGCDITLELYESGELERMVKEA
                     KKA"
     gene            926..>1221
                     /locus_tag="VFF05_09755"
     CDS             926..>1221
                     /locus_tag="VFF05_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZW52137.1"
                     /translation="MSASTGARLPPNWQAPSDYLRERVVLVAGAAGGLGRASALACAR
                     AGASAILLGRRIARLEKVYDEIAALKLAQPALYPLDLAGATPRDYAELAATIER"
BASE COUNT          214 a          382 c          403 g          222 t
ORIGIN      
        1 ggtagtcgat gtagtaggcg tgttcccaga catcgcaggt cagcagcgga cgatcagcgc
       61 cggtgatcgg cgtcgccgcg ttcgaggtgc tgacgatgcc gagcgagcca tccacgcgct
      121 gcaccagcca ggcccagccc gagccgaacg tgccggcggc gagcttgctg aaatcgtcct
      181 tgaactgggc gaagccgccg aacgccttct tgatcgcgtc gccgagcttg ccggacggtt
      241 cgccgccgcc tttcggcgac aagctgttcc agtagaacgt gtggttccag atttgcgcgg
      301 cgttgttgaa catgccgcct tgcgcctttt tgacgatgga cacgaggtcc atggcctcga
      361 attcggtgcc cttgatcagg ttgttgaggt tggtcacgta ggcctggtgg tgcttgccgt
      421 agtgatagtc gatggtttcg gccgaaatat gcggtgccag cgcgtcgcgc gcgtagggca
      481 gggcaggaag ttcgatggcc atggcgttct cctttgattg aaccgaccgt caagtatacg
      541 cgccgcactg ctacaatgcg cagcatcggc gcggatgtcc cgcgcagtct gacgtgaccc
      601 atggacacca tcgaacgcat caagcaaacc gtgcaatcca gtcccatcgt gctcttcatg
      661 aaaggcacgc cgcaattccc gatgtgcggg ttttccagcc gcaccgcgca ggcgctcaag
      721 gcggtcggcg cggagtacgc ctcggtgaac gtactcgagg accccgaagt gcgcgcgaac
      781 ctgccgcact attcgaactg gccgacattc ccgcagttgt tcatcaacgg cgaactgatc
      841 ggcggttgcg acatcacgct ggaactctac gagagcggcg aactcgagcg catggtcaag
      901 gaagcgaaaa aggcctgatc cgcgcatgtc agcctcgacc ggcgcgcgcc tgccgccgaa
      961 ttggcaagcg ccgtccgact atttgcgtga gcgcgtcgtg ctcgttgccg gtgcggccgg
     1021 cgggctgggc cgcgccagcg cgctcgcctg cgcacgcgcg ggcgcgagcg cgatcctgct
     1081 cggccggcgc attgcgcggc tggaaaaggt ctacgacgaa atcgcggcgc tgaagctcgc
     1141 gcagccggcg ctgtatccgc tggatctcgc cggcgccacg ccgcgagact atgcggaatt
     1201 ggcggccacg atcgagcgcg a
//
LOCUS       DASRVO010000790         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_100877, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000790 DASRVO010000000
VERSION     DASRVO010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100877"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFF05_09760"
     CDS             <1..484
                     /locus_tag="VFF05_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZW52138.1"
                     /translation="RAGLPAPVLALSGLRVDWRDKIPEDAQIVVIDGPAGIRSGELAE
                     LAHDVDALLIPLLPSRIDLEASDHFLQEVSRLGRVKRSSLAVGLVGNRLKPWTTASQD
                     AVDEIKRLPFPLVAQLRDTQGYVLVAGLGKSIFDYNSELIRSHQEDWGKLLRWVKKQG
                     "
     gene            493..957
                     /locus_tag="VFF05_09765"
     CDS             493..957
                     /locus_tag="VFF05_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZW52139.1"
                     /translation="MKREILLLRHAHAENAAPGSSDAERNLSLRGIAEARAAGHWLHE
                     QAALPARVLCSPAARARQTLEHALGDIDAQIEERIYEATAGDLDALIDEHADAPRLLL
                     VGHNPGFETFVALLATGQSGDFRGMPPGGIAWFEIDGEAEPGAGTLKAFWSP"
     gene            996..1562
                     /locus_tag="VFF05_09770"
     CDS             996..1562
                     /locus_tag="VFF05_09770"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZW52140.1"
                     /translation="MLCLALNCLAAHAQTPRHEILQLDAARSQAGFTVKVLWLIGVGG
                     EFGAVHGSLDIDNFRGTARVDAHIETDTLRMRSVHNAKWAKSAEFFDATRYPQIDFAS
                     ADFPLVRLRSGGALDGTLTLRGIAKPASFRILPADCARPLAGACAMRAEGSIERSDFG
                     MHSRRGTVADKVALRLQIFVLPPPDPPA"
     gene            1586..3373
                     /locus_tag="VFF05_09775"
     CDS             1586..3373
                     /locus_tag="VFF05_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HZW52141.1"
                     /translation="MALAAFALGACAVDPIHARRAAATAAAAQDHALTCTAADACAIA
                     SPYQDLADAALHESRTGAPVHYVNLLDSGADALLLRVHLIRAARKSIDIQTFIFAQDD
                     AGYLVLDELVKAARRGVKVRVIVDQLFSLDDVELLAQLARAQVNFELRVYNPTFHKAR
                     TPPLDFAAGIVCCFFRFNQRMHNKLFLVDDRIGIAGGRNYENRYFDWDDEFDYRDRDA
                     LVAGPAAQQMAASFERFWRDRRSVPLSRLRDVSSRILDDGLNAPPYRLHAYRNAVRVA
                     ALSRAADDAAYIRAHFAENAMRVGAVEYFSDAPDKFDEPEQPRDDELSRHILELFQDA
                     RSEIVMQTPYLVLSRTAQHAFAEQHRAKPALRIIVSTNSLAATDAFYVYALSYKHKKR
                     FLKLGFEIHEFKPFPQEAAALISDYAVLGDGTEQAQRYRKYGQAPLREHGVRVGLHAK
                     SIVIDGKIALIGSHNFDPRSDHYNTESGFIIHDVAFAQALRASILRDCAPGNAWTIAR
                     RQGTNWLSRLNRAIGDFSARLPLFDLWPYRYASSFELEPGCKPLPPGAPGFYDCYTDV
                     GDFPEVDLPLKTIYTRIVTAFGASLHSIL"
     gene            3440..4003
                     /locus_tag="VFF05_09780"
     CDS             3440..4003
                     /locus_tag="VFF05_09780"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HZW52142.1"
                     /translation="MALEFSIEITDQDLPFFMDAIKRAEQRAAGKSAQQILDEAAKTF
                     AGSQAQQMPEFVRSRLACVENLVAMAADEGWGLSADDKGRVVSALTYFSDPEDLIPDN
                     VPVLGFLDDAIMIEVVQGVLKPEIDAYADFCTYRSQEAAARGADAAKLGREEWLEDRR
                     AELQARMRNRRQSYAPSGNFTPRFKFT"
     gene            complement(4047..>4431)
                     /locus_tag="VFF05_09785"
     CDS             complement(4047..>4431)
                     /locus_tag="VFF05_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008217429.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZW52143.1"
                     /translation="VHGEGLLMAPTIAVSRMLEAAGLTLQDFDYYEIHEAFAAQVLCT
                     LRAWESADYCKNRLGRDKPLGSIDPAKLNVNGSSLALGHPFAATGTRLIATMAKLLAD
                     KGAGRGLISVCTAGGMGVTAIVERP"
BASE COUNT          757 a         1519 c         1386 g          769 t
ORIGIN      
        1 gcgcgccggc ctgcccgcgc cggtactcgc gctgtccggc ctgcgtgtcg actggcgcga
       61 caagattccc gaggacgcgc agatcgtggt catcgacgga cccgccggca tccgtagcgg
      121 cgaacttgcc gagctggcgc acgacgtcga cgcgctgctg attccgctgc tgccctcgcg
      181 catcgatctt gaggccagcg atcacttcct gcaggaagtt tcacgcttgg gccgggtcaa
      241 gcgcagctcg cttgcagtcg gcctggtcgg caaccgcctc aaaccctgga cgaccgcttc
      301 gcaggacgcg gtcgacgaaa tcaagcgcct gccgtttcca ctcgtcgcgc aattgcgcga
      361 cacgcagggc tatgtgctgg tcgcgggtct gggcaagagc atttttgact acaactccga
      421 gctgatccgc agccatcagg aagactgggg caaactgctg cgctgggtca agaaacaggg
      481 ctgaacgaaa cgatgaaacg cgaaatactg ttgttgcgcc atgcccacgc cgagaacgcc
      541 gcacccggaa gcagcgacgc cgaacgcaat ctgagcctgc gcggcattgc cgaagcgcgc
      601 gccgccggcc attggctgca cgagcaggcc gcgttgccgg cacgcgtgct gtgctcgccc
      661 gccgcgcgcg cacgccagac gctggaacac gcgctcggcg acatcgacgc gcagatcgag
      721 gaacgcatct acgaagccac cgccggcgac ctggatgcgc tgatcgacga gcatgccgac
      781 gcgccacgcc tgctcttggt cggccacaat cccggcttcg agactttcgt cgccctgctc
      841 gctaccgggc agtcgggcga cttccgcggc atgccgccgg gcggaatcgc ctggttcgaa
      901 atcgacggcg aagccgaacc cggcgcgggg acattgaaag ctttctggtc cccataagct
      961 gatcttccat gcgtacgctg cggatacggc tctgcttgct gtgtctggcg ctgaactgtc
     1021 tcgccgcgca cgcgcagacg ccgcgccacg aaatcctgca attggatgcc gcgcgctcgc
     1081 aggccggatt cacggtcaag gtgctgtggt tgatcggcgt cggcggcgaa ttcggcgcgg
     1141 tccacggcag cctggacatc gataactttc gcggcacggc gcgcgtggat gcgcatatcg
     1201 agaccgacac cctgcgcatg cgcagcgtgc acaacgcgaa atgggcgaag tcggcggagt
     1261 tcttcgatgc gacgcgttat ccgcaaatcg atttcgcgtc cgcggatttc ccgctcgtgc
     1321 gcctgcgcag cggcggcgct ctggacggca cgctcacctt gcgcggcatc gccaagccgg
     1381 caagtttccg catcctgccc gcggattgcg cccggccgct cgccggtgcc tgcgccatgc
     1441 gcgccgaagg cagcatcgag cgcagcgatt tcggcatgca ctcgcgacgc ggcaccgtcg
     1501 ccgacaaggt ggcgctgcgt ctgcaaattt tcgtgctgcc accgccggac ccgccagcat
     1561 gaggcgcact gccgcattcg cgatcatggc acttgccgcg ttcgcgctcg gcgcctgcgc
     1621 ggtcgatccg atccacgcgc gccgcgctgc ggcgacggcc gccgccgcgc aggatcacgc
     1681 tctcacctgc accgcggccg acgcctgcgc gattgcctcg ccgtaccagg atctggccga
     1741 cgcggcgttg catgaatcgc gcacgggtgc gccggtgcac tacgtcaacc ttctcgacag
     1801 cggcgccgat gcgttgctgc tgcgcgtcca cctgatccgc gccgcgcgca agagcatcga
     1861 catccagacc ttcatcttcg cgcaggacga cgccggctac ctcgtgctcg acgaactggt
     1921 caaggcggcg cggcgcggcg tcaaggtgcg cgtgatcgtc gatcaattgt tttcgctcga
     1981 cgacgtggaa ctgctcgccc agctcgcgcg cgcgcaggtg aacttcgagc tgcgcgtgta
     2041 caacccgact ttccacaagg cgcgcacgcc gccgctggat ttcgccgccg gcatcgtgtg
     2101 ctgttttttc cggttcaacc agcgcatgca caacaagctg ttcctcgtcg acgaccgcat
     2161 cggcatcgcc ggcgggcgca attacgaaaa ccgttacttc gactgggacg acgaattcga
     2221 ctatcgcgac cgcgacgcgc tcgttgccgg tcccgccgcg caacaaatgg ccgcttcgtt
     2281 cgagcgtttc tggcgcgatc gccgcagcgt gccgctgtcg cgcctgcgcg acgtgtcctc
     2341 gcgcatcctc gacgacggcc tgaacgcgcc gccttaccgc ctgcacgcgt atcgcaacgc
     2401 ggttcgggtg gcggcgctgt cgcgcgcggc ggacgacgcc gcctacattc gcgcgcattt
     2461 cgcggagaac gcgatgcgtg tcggcgcggt ggaatacttt tccgacgcgc cggacaagtt
     2521 cgacgaaccc gagcagccgc gcgacgacga actgagccga catattctgg aactgttcca
     2581 ggacgcgcga tccgaaatcg tcatgcagac gccgtatctc gtgctcagcc gcacggccca
     2641 gcacgcgttc gccgagcagc atcgcgccaa accggcattg cggatcatcg tatcgaccaa
     2701 ttcattggcc gcgacagacg cgttctacgt ttacgcgttg tcgtacaagc acaagaagcg
     2761 ttttctcaaa ctcggcttcg agatccacga attcaagccg tttccgcaag aggccgcggc
     2821 gctgatttcg gattatgccg tgctcggcga cggcaccgag caagcgcagc gctatcgcaa
     2881 gtacggacag gcgccattgc gcgagcacgg cgtgcgcgtg ggtctgcatg ccaagtccat
     2941 cgtcatcgac ggcaaaatcg cgctgattgg ttcgcacaat ttcgatccgc gctcggatca
     3001 ttacaacacc gaatccggat tcatcatcca cgacgtcgcg ttcgcgcagg cactgcgcgc
     3061 ctccatcctg cgcgattgcg cgcccggcaa cgcctggacg atcgcgcgcc ggcagggcac
     3121 caactggctc agtcgcctga atcgcgcgat cggcgatttc tcggcgcggc ttccgctgtt
     3181 cgacctgtgg ccctatcgtt atgcgtcgag cttcgaactg gagcccggct gcaagccgct
     3241 gccgccgggc gcgccgggct tctacgactg ctacaccgac gtgggcgatt tcccggaagt
     3301 cgacctgccc ctcaagacca tctacacgcg catcgtcacg gccttcgggg caagtcttca
     3361 ttcgattctg tgagccaatt gacgttatcg ccccggctgg ctaggctgcg cgttccgcca
     3421 cccatttcag gacgccgcca tggcccttga gttttccatc gagatcaccg accaggacct
     3481 gccgtttttc atggacgcga tcaagcgggc cgagcagcgt gcggccggca agagcgcgca
     3541 gcagatcctg gacgaggcgg cgaagacctt tgccggctcg caggcgcagc agatgccgga
     3601 attcgtgcgt tcgcgcctgg cctgcgtgga aaacctcgtc gccatggccg ccgacgaagg
     3661 ctggggcctg tccgccgacg acaagggccg cgtggtttcc gcgctgacct atttttccga
     3721 cccggaagac ctcattccgg acaacgtgcc ggtgctgggc ttcctcgacg acgcgatcat
     3781 gatcgaagtt gtgcagggcg tgctcaaacc ggagatcgac gcctacgcgg atttctgcac
     3841 ctatcgcagc caggaagccg ccgcgcgcgg cgccgacgcg gccaaactcg gccgcgagga
     3901 atggctggaa gatcgccgtg ccgaactgca ggcgcgcatg cgcaaccggc gccagtccta
     3961 cgcgccgagc ggcaatttca cgccgcgctt caagttcacc tgaggcttga tttgcacccg
     4021 gcggagcgtc gcgcgcaacc ggtagctcaa ggccgctcca cgatcgcggt cacgcccatg
     4081 ccgccggcgg tgcagacgga aatcaggccg cggcccgcgc ccttgtctgc cagcaatttc
     4141 gccatcgtcg cgatgaggcg cgtaccggtt gcggcaaacg gatggccaag cgccagactc
     4201 gaaccattga cgttgagctt ggccggatcg atggagccca gcggcttgtc gcggccgagg
     4261 cgattcttgc aatagtccgc gctctcccac gcgcgcagcg tgcacagcac ctgtgcggcg
     4321 aaagcttcgt ggatttcgta gtagtcgaaa tcctgcaagg tcaatccagc ggcttcgagc
     4381 atgcgcgata cggcgatggt cggcgccatc agcaggcctt cgccgtgcac g
//
LOCUS       DASRVO010000791         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_231589, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000791 DASRVO010000000
VERSION     DASRVO010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231589"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1684)
                     /locus_tag="VFF05_09790"
     CDS             complement(116..1684)
                     /locus_tag="VFF05_09790"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HZW52144.1"
                     /translation="MGAFGALRDLRPTVARIRSVARERARRPGDGRTLHSHLIVGFGI
                     ILALLAALTVVAALFTQGMEQQIVLACGTCAIVAGVLAANWSTRRTTGPIRAALAAAQ
                     RITAGDLTSAVEVGAGGEIGALMHALAQMQARMFQIVSEVRTGTTTVAGTSSQITRDN
                     ESLSERTQGQAASVQQTVSSMEQITSTVHRNAQDAQQANALVVTASQQAIRGGAAMAE
                     VVDTMGAIKGSSRKIGDIISVVDAIAFQTNLLALNAAVEAARAGEQGRGFAVVAGEVR
                     TLSRRVADAAGEIKALIGESVKNVELGGRLVDDAGRTIGEVVASVKQVEDIMGRINTA
                     SQQQSSGIESINAAVAKIDRMTRRNSALVEDVRKTATSLNEQSVALMRTVTGFDLGER
                     EFGNADEAVALVQGACAFLASHGKDALLADINRLGKGRFVDRDLYLMVIGAADAVFVA
                     HGNNPRVLGLGPKSTDVDGRLFVQEMAQAARVRGEGWIDYKWAHPVTNEIRTKTSFFK
                     RAGDIVIACGIYKA"
BASE COUNT          295 a          700 c          657 g          345 t
ORIGIN      
        1 ggtgtcgcgc tcgaagcagc ttgccgtcta tcgcgtggat gtgaagcgcg ccgatggcac
       61 ggcgatctcc agcttcaccg gcacggtgta cgtgacgcag cggccgacgc cctgatcacg
      121 ccttgtagat gccgcaggcg atgacgatgt cgccggcccg cttgaagaag gacgtcttgg
      181 tgcggatctc gttggtgacc gggtgcgccc acttgtaatc gatccagcct tcgccgcgga
      241 cccgggccgc ctgcgccatc tcctgcacga acagacggcc gtcgacgtcc gtgctcttcg
      301 gacccagccc cagcacacgc ggattgttgc cgtgggcgac gaacaccgcg tcggctgcgc
      361 cgatgaccat caggtacagg tcgcggtcga cgaagcggcc cttgccgagg cgattgatgt
      421 ccgccagcag cgcgtccttg ccatgacttg ccaggaaggc gcacgcgccc tgtaccagcg
      481 ccaccgcctc gtcggcattg ccgaactcgc gctcgcccag atcgaagccg gtcaccgtcc
      541 gcatcagcgc gaccgactgc tcgttgagcg acgtggccgt cttgcgcacg tcctccacca
      601 gtgccgaatt gcgccgcgtc atccggtcga tcttggcgac cgcggcgttg atcgattcga
      661 tgccgctgct ttgctgctgg ctggccgtgt tgatccggcc catgatgtcc tcgacctgct
      721 tcacggaagc gacgacctcg ccgatcgtgc gacccgcgtc gtcgaccagg cgcccgccga
      781 gctcgacgtt cttcactgat tcgccgatca gcgccttgat ctcgccggcg gcgtcggcga
      841 cgcgcctgga cagcgtgcgc acttcgccgg ccaccacggc gaagccgcgg ccctgctcac
      901 ccgcgcgcgc cgcctcgacg gcggcattga gcgccagcag gttggtctgg aacgcaattg
      961 cgtcgaccac gctgatgatg tcgccgatct tgcgcgagct gcccttgatg gcgcccatcg
     1021 tgtcgacgac ctcggccatg gccgcgccgc ccctgatcgc ctgctgcgat gcggtcacga
     1081 cgagcgcgtt ggcctgttgc gcgtcctgcg cgttgcggtg gaccgtggac gtgatctgct
     1141 ccatcgacga gaccgtctgc tgcaccgagg ccgcctgccc ctgcgtgcgc tcggacagcg
     1201 actcgttgtc gcgcgtgatc tgggacgagg tgcccgcgac cgtggtcgtt ccggtgcgca
     1261 cctcgctgac gatctggaac atgcgcgcct gcatttgtgc cagcgcgtgc atcagcgcgc
     1321 cgatttcgcc gcccgcgccg acctcgacgg cgctggtgag gtcgcccgcg gtgatgcgct
     1381 gcgccgcggc cagcgcggcc cggatcggac cagtggtgcg gcgcgtcgac cagttcgcgg
     1441 ccagcacgcc tgcgacgatc gcgcaggtcc cgcaggccag cacgatctgc tgttccatcc
     1501 cttgtgtgaa cagcgcggcg accacggtca gcgcggcgag cagcgcaagg atgatgccga
     1561 aaccgacgat cagatgcgag tgcaacgtcc gtccgtcgcc cggccgacgg gcacgctcgc
     1621 gtgcaacgga acgaatacgg gcgacggtcg gccgcagatc gcgaagcgcg ccgaacgcgc
     1681 ccatcttcaa ggctggggtg gtcatcgggt ccctcgttct ggctcctcgg agcgaatggc
     1741 cggcgctcgg cttatgtccc ctgatccgcc gcttttactc gaagcggcgc ctcgcgtcac
     1801 gcattgctgc gacgcacact gtgcaatagt ggcgcaattg ccgctcgaaa agcgcccgcg
     1861 gccgccgtcg gcttcaggaa atgatgtagg cggccgagcc ggtcgacacg agcttgccgt
     1921 cggcgtcgtg gaattccatg cgtgtcgagg caacgcgcga gccgagtcgc agcacattcg
     1981 cggacagctc gaaatga
//
LOCUS       DASRVO010000792         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_257030, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000792 DASRVO010000000
VERSION     DASRVO010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257030"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFF05_09795"
     CDS             <1..278
                     /locus_tag="VFF05_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HZW52145.1"
                     /translation="SPGAFRPPPKVDSAVVRLAPLPASALPDVDFAGIANVVRAAFGQ
                     RRKTLANALSKIMGADDIAAAGIDPRARAEQLPPSDFVALAKHAGRE"
     gene            437..820
                     /gene="apaG"
                     /locus_tag="VFF05_09800"
     CDS             437..820
                     /gene="apaG"
                     /locus_tag="VFF05_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HZW52146.1"
                     /translation="MSSTTPYDIRVDVRTRFLDDESAPAENRYVFAYTIRIANAGSVP
                     AKLLTRHWIITDANGRVQEVRGDGVVGEQPYLQPGENFEYTSGAILETAVGTMHGSYQ
                     MLADDGRRFDAAIAPFTLSIPRTLH"
     gene            820..1668
                     /locus_tag="VFF05_09805"
     CDS             820..1668
                     /locus_tag="VFF05_09805"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HZW52147.1"
                     /translation="MATWAIGDIQGCHDELTRLLEKIRFDPAHDMLWFCGDLVNRGGQ
                     SLAVLRLVKSFGERAIVVLGNHDLSLLAVAERNEADQAKVNPELREVLFAPDRNELLD
                     WLRARPLVHVDRALDFMLVHAGLAPPWRVDDAERAAREVEQRLRGDGARGLLLSMFGN
                     KPDRWHPRLQGMDRLRAAINVMTRLRYCDVRGRIAYREKGVPGTQKPGLYPWFEVPGR
                     VERGIRIVCGHWSALGRFQGMGVHAIDGGCVWGGELIALRLDSEEPQFVALKSTRPRP
                     PGKFIE"
     gene            complement(1673..>1924)
                     /locus_tag="VFF05_09810"
     CDS             complement(1673..>1924)
                     /locus_tag="VFF05_09810"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477647.1"
                     /note="catalyzes the formation of S-formylglutathione from
                     S-(hydroxymethyl)glutathione; also catalyzes the formation
                     of aldehyde or ketone from alcohols; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HZW52148.1"
                     /translation="IGVAGAGQEISTRPFQLVTGRVWRGSAFGGVKGRSQLPGYVDRY
                     LRGEIKIDEMVSAVMPLEKINEAFDLMHEGKTIRSVIRY"
BASE COUNT          329 a          646 c          610 g          339 t
ORIGIN      
        1 tttcgcccgg cgcgtttcgc ccgccgccaa aagtcgattc ggccgtggta cggctcgcac
       61 cgctgccggc ctccgcgctg ccggacgtcg atttcgccgg catcgcaaat gtcgtccgcg
      121 ccgccttcgg ccagcgccgc aagaccttgg cgaatgcact ctccaaaatc atgggcgccg
      181 acgacatcgc cgccgccggc atcgatccgc gcgcgcgcgc cgagcagttg ccgccatccg
      241 atttcgtggc gctggcgaaa cacgcaggac gggagtagcg aagcggctct caacagcgga
      301 gctggtcatc ccgccgttcg cggttccggc gcgatacgcc gctgcgcggc ttctcccgcc
      361 ctacccgtcg aggcagcgga tttcgacgtc cgttgaaagg cttgcccgat ccgccgccgt
      421 gccggacaat cccggcatgt cttccactac gccctacgat atccgcgtcg acgtgcgcac
      481 gcgcttcctc gacgacgaat ccgcgcccgc ggaaaaccgt tacgtcttcg cctacacgat
      541 ccgcatcgcc aatgccggca gcgtaccggc gaaattgctg acccggcact ggatcatcac
      601 ggatgcgaac ggccgtgtgc aggaagtgcg cggcgacggc gtcgtcggcg aacaaccgta
      661 cctgcagccc ggcgagaatt tcgaatacac ctccggcgca atcctggaaa ccgcggtcgg
      721 caccatgcac ggcagctacc agatgttggc cgatgacggc cgccgcttcg acgccgccat
      781 cgcgccgttc acgctttcga ttcccagaac gttgcactga tggcaacctg ggcgatcggc
      841 gacatccagg ggtgtcacga cgaactgacc cgcctgctgg aaaaaatccg cttcgatccg
      901 gcgcacgaca tgctgtggtt ttgcggcgat ctggttaacc gcggcggcca gtcgctggcg
      961 gtattgcggc tggtgaaatc ctttggcgaa cgcgccatcg tcgtactcgg caaccacgac
     1021 ttgtcgctgc ttgcggtcgc cgaacgcaac gaggcggatc aggccaaggt caatccggaa
     1081 ttgcgcgaag tgctgttcgc gccggatcgc aacgagctgc tcgactggct gcgcgcgcgg
     1141 ccgctcgtgc atgtggatcg tgcgctggat ttcatgctcg tgcatgccgg tcttgcgccg
     1201 ccatggcgcg tcgatgatgc cgagcgcgcg gcgcgcgaag tcgagcagcg gttgcgtggc
     1261 gacggcgcac gcggattgtt gctgtccatg ttcggcaaca agccggaccg ctggcacccg
     1321 cgcctgcaag gcatggatcg actgcgcgcg gcgatcaacg tgatgacgcg cctgcgctat
     1381 tgcgacgttc gcggccgcat cgcctaccgc gaaaaaggcg tgccgggcac gcaaaagccc
     1441 ggcctgtacc cgtggttcga agtaccgggt cgcgtcgaac gcggcatccg catcgtgtgc
     1501 gggcactggt cggcgctcgg acgcttccag ggcatgggcg tgcatgccat cgatggcggt
     1561 tgtgtctggg gcggcgagct gatcgcgctg cgcctggaca gcgaggaacc gcaattcgtc
     1621 gcgctcaagt ccacgcgacc gcggccgccg ggcaagttca tcgaatgaac tgtcagtagc
     1681 ggattacaga tcgtatggtc ttgccttcgt gcatcaaatc gaaggcttcg ttgatttttt
     1741 ccagcggcat caccgcactg accatttcgt cgatcttgat ctcgccgcgc aggtagcgat
     1801 cgacgtaacc gggcaactgc gaacgccctt tcacaccgcc gaaagccgag ccacgccaga
     1861 cgcggccggt gaccagctgg aacgggcgcg tggaaatttc ctgcccggcg ccggcaacgc
     1921 cgat
//
LOCUS       DASRVO010000793         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_257063, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000793 DASRVO010000000
VERSION     DASRVO010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257063"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1482)
                     /locus_tag="VFF05_09815"
     CDS             complement(1..1482)
                     /locus_tag="VFF05_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52149.1"
                     /translation="MYRFPSLPCKLLFVAAAGFAQSAAADATRTTPDASEMSQSEWRH
                     LQLQAADQSDKIMRDARKQSGLLAQYLYMQAHYDANHERAFQIIFGQYLSWFQTFIGD
                     YDGAAASFSIAQPAQPDDARTPLGGGTFLPETSAPPNLKDLRHPVGTGTDSSAAGSVH
                     TGAWRVQPAADVILELAKDRKAVFFNEAHSVPLTRTLTIELLAKLRAEGFNYFAAETL
                     YRNDKALNQRGYPTPDSGFYIDEPLYGEMVRTALKLGFKVVAYDVENAGAGDPRELSG
                     AQNLYHVFKQDPNARLIVDAGFAHIQKTGKYLGGSSMAEFFEKMSGIDPLCIEQTMMI
                     QHARPDQDHPYYTAIVDAFHMSRPIVFVDGKQTWTLKPKEYDLSVVFPPSPHNDLRPD
                     WLSLGGLRVAYPVAGDICRGHFPCLIEARYANEGADAVAADRTLLNVIQPGADLAERV
                     LSDHGTAKSRLFLRPGKYRITAVDRSNHVQTSREVTISASAKP"
     gene            complement(1485..>2040)
                     /locus_tag="VFF05_09820"
     CDS             complement(1485..>2040)
                     /locus_tag="VFF05_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZW52150.1"
                     /translation="FKSAFVEKVRALRVGDPLDAANDLGALVSRAHFDKVMGHIALAR
                     EEGGTILCGGDSVHLAGRCANGWFVAPTVIEGLGADCRTNQEEIFGPVATLIPFDGED
                     AAVAIANATNYGLAASVWTTNLARAHRVGARLESGIVWINCWLKRDLRTPFGGVKQSG
                     VGREGGVDALRFFTEAKNVCINLE"
BASE COUNT          342 a          616 c          681 g          401 t
ORIGIN      
        1 tcatggcttc gcacttgctg aaatagtgac ttcgcgcgag gtctgcacat ggttgctgcg
       61 gtcgaccgca gtgatgcggt acttgccggg ccgcaggaac agacggcttt tcgccgtgcc
      121 gtgatcggac aaaacgcgct ctgcgaggtc ggcgccgggc tggatgacgt tgagcagggt
      181 gcggtcggcg gccacggcat ccgcgccttc attcgcgtag cgcgcttcga tcaggcacgg
      241 aaagtgccca cggcaaatgt cgcctgcgac cgggtacgct acgcgcagcc cgcccaggct
      301 cagccagtcc ggtcgcaggt cgttgtgcgg cgaaggtgga aacaccacgc tcaaatcgta
      361 ttctttcggc ttcagcgtcc aggtctgctt gccgtccacg aacacgatcg gccgactcat
      421 gtggaaggcg tcgacgatgg cggtgtagta gggatgatcc tgatcgggcc gcgcatgctg
      481 gatcatcatg gtctgttcga tgcatagcgg atcgatgccg gacatcttct cgaaaaactc
      541 ggccatgctg ctgccgccga ggtatttgcc ggtcttctgg atgtgcgcga agccggcatc
      601 aacgatgagg cgcgcgttcg gatcctgctt gaagacgtga tacaggtttt gcgcgccgga
      661 cagttcgcgc ggatcgccgg cgccggcgtt ttccacgtcg taggcgacga ccttgaagcc
      721 gagcttgagc gcggtgcgca ccatctcgcc gtacagcggc tcatcgatgt agaagccgct
      781 gtcgggcgtc ggatagccgc gttggttgag cgccttgtcg ttgcgataca gggtttcggc
      841 tgcgaagtaa ttgaaaccct cggcgcgcag tttcgccagc agttcgatgg tcagcgtacg
      901 cgtcaacggc acgctgtgcg cctcgttgaa gaaaacggcc ttgcggtcct tggccagttc
      961 gaggatcacg tccgccgcgg gttggacgcg ccaggcgccg gtgtgcacgg acccggctgc
     1021 ggaagagtcc gtcccggttc ccacgggatg tctgaggtct ttcaagttgg gaggcgcgga
     1081 tgtttccggc aggaacgttc cgccgccaag cggcgtgcgc gcatcgtccg gctgcgccgg
     1141 ctgggcgatg gaaaagctcg ccgccgcgcc gtcgtagtcg ccgatgaaag tctggaacca
     1201 cgacaggtac tggccgaaga tgatctggaa cgcgcgttcg tgattggcgt cgtaatgcgc
     1261 ctgcatgtac aggtattgcg cgagcagacc ggactgtttg cgcgcgtcgc gcatgatctt
     1321 gtccgattgg tcggccgcct gcaattgcag gtgccgccat tccgactgcg acatttcgct
     1381 ggcgtcgggt gtggtgcgcg tggcgtccgc cgcagcggat tgcgcgaatc cggcagcggc
     1441 cacgaacagc aatttgcacg gtaggctggg gaatctgtac atgttcactc cagattgatg
     1501 cacacgtttt tcgcttcggt gaagaatctc agcgcatcca cgccgccttc gcggccgacg
     1561 ccggattgct tgacgccgcc gaacggcgtg cgcaggtcgc gttttagcca gcagttgatc
     1621 cagacgatgc cggattccag gcgtgcgccg acgcggtgcg cgcgcgccag gttcgtcgtc
     1681 catacggacg cggcaaggcc gtaattcgtc gcgttggcga tggcgaccgc cgcgtcctcg
     1741 ccgtcgaacg gaatgagtgt ggcaaccggg ccgaagattt cttcctggtt cgtgcggcaa
     1801 tccgcgccga gcccttcgat tacggtcggg gcgacgaacc agccgttcgc gcaacgacct
     1861 gcgagatgca ccgaatcgcc gccgcacaag atggtgccgc cttcctcgcg cgcgagcgcg
     1921 atgtggccca tgaccttgtc gaaatgcgcg cgtgagacca gcgcgcccag atcgttcgct
     1981 gcatccagcg gatcgccgac gcgcaaggcg cggactttct ccacgaacgc ggacttgaat
//
LOCUS       DASRVO010000794         1002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_284462, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000794 DASRVO010000000
VERSION     DASRVO010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284462"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1002
                     /locus_tag="VFF05_09825"
     CDS             <1..>1002
                     /locus_tag="VFF05_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HZW52151.1"
                     /translation="MMATLRCDHPDIETFVDAKRNPAVLRHFNLSVLVSDAFMDAVAE
                     DRDWPLVFPGLADTRTVKARDLWQRILRAAYDTAEPGVLYVDTINRDNTLGHLENLSA
                     TNPCGEIPLPPYGACDLGSLNLTAFVAAPFAADARLDFSALADATGIAVRFLDNVIDV
                     SRYPLPAQAQEARRKRRIGLGLTGLADALVLLGLRYDSAAAQRLAAQAMQAVRDHAYQ
                     ASVELAHEKGAFPAFERDAFLASGFATRLPAGIRDAIAAHGIRNSHVLAIAPAGTISL
                     LANNLSSGIEPIFSAEAERRVLEADGRYRTHRVVDYACQRWRQIDRGLPPAFVEAQE"
BASE COUNT          157 a          376 c          312 g          157 t
ORIGIN      
        1 gatgatggcg acgctgcgct gcgaccaccc cgacatcgaa accttcgtcg acgccaagcg
       61 caaccccgcg gtgctgcggc atttcaacct gtcggtgctg gtaagcgacg ccttcatgga
      121 cgcggtggcc gaggaccgcg actggccgct ggtattcccc ggcctcgccg acacgcgcac
      181 ggtgaaggca cgcgacctgt ggcagcgcat cctgcgcgcg gcctacgaca cggccgaacc
      241 cggcgtgctg tacgtcgata ccatcaaccg cgacaacacg ctgggccacc tggaaaacct
      301 gagcgccacc aatccctgcg gcgaaatccc gctgccgccc tacggcgcct gcgatctcgg
      361 ctcgctcaac ctgaccgcct tcgtggctgc gccctttgcc gccgacgccc gcctcgattt
      421 ttccgccctg gcggacgcga ccggcatcgc ggtgcgcttt ctcgacaatg tcatcgacgt
      481 ctcgcgctat ccgctgccag cgcaggcgca ggaagcgcgc cgcaagcggc gcatcggtct
      541 cggcctcacc gggctggcgg acgcgctggt gctgctgggc ctgcgctacg acagtgcagc
      601 tgcgcaacgc ctggccgcgc aggcgatgca ggcggtacgc gaccatgctt atcaggcgtc
      661 ggtcgaactc gcgcacgaga aaggcgcgtt tcctgccttc gagcgcgacg ccttcctggc
      721 gagcggtttc gccacccgcc tgccggccgg catccgcgac gccatcgccg cacacggcat
      781 ccgcaacagc catgtattgg ccatcgcgcc ggccggcacc atcagcctgc tcgccaacaa
      841 tctttccagc ggcatcgagc cgattttctc ggccgaagcg gaacgtcgcg tgctggaggc
      901 ggacggccgt taccgcaccc atcgcgtcgt cgattacgcc tgccagcgat ggcggcagat
      961 cgaccgcggc ctgccgcccg ccttcgtcga ggcgcaggag at
//
LOCUS       DASRVO010000795         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_75769, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000795 DASRVO010000000
VERSION     DASRVO010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75769"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFF05_09830"
     CDS             complement(<1..287)
                     /locus_tag="VFF05_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52152.1"
                     /translation="MNAIPDIGIEGIGVWSPELPDWPVAKRILVDNVPTVPAAPARPT
                     AAALAAGERRRAPESVLIAVEAAAQACAMAGRDPRDLAHVFGSAYGDLAIS"
     gene            complement(284..1483)
                     /locus_tag="VFF05_09835"
     CDS             complement(284..1483)
                     /locus_tag="VFF05_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HZW52153.1"
                     /translation="MPSRISPLAITAFTVTCAAGRGRDAFACALQSRTSGLRENDLPE
                     WPLPCQVGRVSGIENAPLPAAWAEWESRNHRLAWMALNEDGFRERALAAIERYGAPRV
                     ALILGTSTSSIGETEHAYRELGADGRFVPAWQHRRVHETHALAAFVRQALQLRGPSMT
                     IATACSSSAKVFASAQRLIQAGIVDAAIVGGADTLCGSVLYGFNALELVSREPCRPFD
                     VARSGLSLGEAAGFALLESISVDKSALRLTGYGESADAHHMSAPHPQGLGAERAMREA
                     LARAGIAAGEVDYINLHGTASRQNDEVEAMLVARLFPASVHASSTKGWTGHTLGAAGI
                     VEAVAALLAFERDLAPGTLNTRHLDPACGPQIRIDNAQRRVRVALSNSFGFGGNNCCL
                     AFAAGAA"
     gene            1611..2009
                     /locus_tag="VFF05_09840"
     CDS             1611..2009
                     /locus_tag="VFF05_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52154.1"
                     /translation="MSRLRFALLLLPVALLLMAFHQAPLVNPPSIAVPPKDTPDQVNK
                     SIRAALLSGQWLVTANNPGEIDGTLARNDYSANIAVSYDDKKIQIRYVDSSNLKYEKK
                     NGVEYIHRNYLVWIRDLAAQIQSNLLLFGS"
     gene            2017..>2192
                     /locus_tag="VFF05_09845"
     CDS             2017..>2192
                     /locus_tag="VFF05_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008437449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HZW52155.1"
                     /translation="MRERAAEVEAYLRGLQDRICAALETADGGARFVEDAWTRPHAGT
                     DLRGGGRTRVLKDGG"
BASE COUNT          389 a          737 c          706 g          360 t
ORIGIN      
        1 tcgctgatcg cgagatcgcc atacgccgag ccgaaaacgt gcgccaggtc gcgtggatcg
       61 cggcctgcca tcgcgcaagc ctgcgccgcg gcctccaccg caatcagcac cgattccggc
      121 gcacgccgac gttcgcccgc cgcgagtgcg gccgcggtcg gtcgcgccgg tgcagcggga
      181 acggtaggca cattgtccac aagtatacgt tttgcgaccg gccaatccgg caactccggc
      241 gaccacacgc cgatgccttc gatgccgatg tcaggtatcg cgttcatgcc gcacccgccg
      301 cgaacgcgag gcagcagttg ttgccgccga agccgaacga gttggacaac gccacgcgca
      361 cccggcgctg cgcgttgtcg atgcggattt gcggcccgca ggccggatcg agatgtcgag
      421 tattcagcgt gccgggcgcc aggtcgcgtt cgaaggcgag cagcgccgcc acggcctcaa
      481 cgatgccggc cgcgccaagc gtatgtccgg tccagccttt ggtcgaactc gcgtgcacgg
      541 acgccgggaa caggcgcgca accaacattg cttccacttc atcgttctgc cggctcgccg
      601 tgccgtgcag gttgatgtag tcgacctcgc ccgctgcgat gcccgcacgc gccagcgcct
      661 cgcgcatcgc ccgttcggca cccagtccct gcggatgcgg cgccgacatg tggtgcgcat
      721 cggccgactc accgtagccg gtcaggcgta gtgcggattt gtccacggat atgctttcga
      781 gcaacgcgaa gccggccgcc tcgcccaacg acagtccgga acgcgccacg tcgaatggtc
      841 ggcacggctc gcgcgacacc agctccagcg cgttgaagcc gtataaaacg ctgccgcaca
      901 gcgtgtccgc gccgccgacg atggcagcat cgacgatgcc tgcctggatc aggcgctgcg
      961 cgctagcgaa caccttggcg ctggacgaac aagccgtggc gatggtcatg ctcggaccgc
     1021 gcagttgcag agcctgccgc acgaaggcgg cgagcgcatg cgtctcatgc acgcggcgat
     1081 gctgccacgc cggaacgaag cggccatcgg cgccaagttc gcggtaggca tgttcggttt
     1141 cgccgatgct cgacgtcgag gttcccagga tcaacgccac gcgcggcgca ccgtagcgtt
     1201 cgatcgcggc aagcgcgcgt tcgcggaaac catcctcgtt caacgccatc caggcgaggc
     1261 gatgattgcg cgattcccac tccgcccatg ccgccggcaa cggcgcattc tcgatcccgg
     1321 acacgcgccc gacctggcac ggcagcggcc attcgggcag gtcgttttcg cgcaggccgg
     1381 atgtgcgcga ttgcagtgcg cacgcgaacg catcccggcc gcgtccggcg gcgcaggtga
     1441 cggtgaacgc ggtgatggcg agcggcgaaa tgcgggacgg caagcgtgga cacgggcgcg
     1501 acaacgggcg gcgagtatag attccgccgc gctgcgcccg caatgcgcac gtttgttacc
     1561 attgccgcgc cgtggatttc ggcgttcccg catcgaacaa ggagattgcg atgtcgcgtt
     1621 tgcgttttgc cttgctgttg ctccccgttg cgctcctgct catggcgttc caccaggcgc
     1681 cgctggtcaa tccgccatcg atcgcggtgc cgcccaagga cacgccggat caggtgaaca
     1741 aatccatccg ggctgctttg ttgagcggac aatggctggt gaccgcgaac aaccctggcg
     1801 aaatcgacgg cacgctcgca cgcaacgatt attcggcgaa tatcgccgtt tcctacgacg
     1861 acaagaaaat ccagatccgt tacgtcgaca gttccaatct caagtacgag aagaaaaacg
     1921 gggtcgagta catccacagg aactatctgg tctggattcg cgatctcgct gcgcaaatcc
     1981 agtcgaatct gctcctgttc ggcagctgaa gcaccgatgc gcgaacgcgc cgccgaggtt
     2041 gaagcctacc tgcgcggctt gcaggatcgc atttgcgccg cactcgaaac tgccgatggc
     2101 ggtgcgcgtt tcgtggaaga tgcgtggacg cgtccgcacg ccggcacgga cctgcgcggc
     2161 ggcggacgca cgcgcgtgct caaggatggc gg
//
LOCUS       DASRVO010000796         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_23879, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000796 DASRVO010000000
VERSION     DASRVO010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23879"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="VFF05_09850"
     CDS             complement(<1..1443)
                     /locus_tag="VFF05_09850"
                     /inference="COORDINATES: protein motif:HMM:NF023683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52156.1"
                     /translation="MQTRLSLLLALSSSLLLSACGGGSNAPHGSDSGANNSGGSPVTA
                     VITARFDPTNAVVPFPTNLLLSGTTDLTLNIPVADPANLSDPQVALNALDGFSTTAPW
                     STTFSTAPKPSTIIPGPGGTVRVFQVSLTGPGGGVTGITRELKAPQDFVALISPSDSS
                     GRTLAIVPTRPLQQLTSYMVVLIGNGGQLNPAPSPSITDGTGNDVTPDTTYFLAKRTS
                     ALCVNGQSTDPLLPASQACALEPLRQLVNSQEYAVDQFDGAATDQRIVLSWVATTQAI
                     TPVMQAVQGRVEQTPAAQATLAPTGLDLSKLGVGLPPVADIYIGVLPVPYYLSAPSAA
                     DPLAPLHGFWHAAAGAYVPPFNAAGLDPTSTLVTFANPFPVATSTQLIPVLLTVPNAN
                     SGKAKPAAGWPVVIFQHGITRNRTDAFAIAGTLAAQGFAVVAIDLPLHGITDTSNPFY
                     IGNTPLAALGAHERTFNLDLENNSTGAPGPD"
BASE COUNT          289 a          462 c          577 g          285 t
ORIGIN      
        1 gtccggaccc ggcgcgccgg tcgagttgtt ctcgagatcg agattgaacg tgcgctcgtg
       61 cgcgccgagc gcggcgagcg gcgtgttgcc gatgtagaac ggattcgacg tgtcggtaat
      121 gccgtgcagc ggcagatcga tcgcgacgac cgcaaagccc tgcgcggcca gcgtgccggc
      181 aatcgcgaac gcatcggtgc ggttgcgcgt gatgccgtgc tggaagatca ccaccggcca
      241 gccggccgcc ggcttggcct tgccggaatt cgcattcggc acggtcagca gcaccgggat
      301 gagctgggtc gaagtcgcca ccggaaatgg atttgcgaag gtgaccaggg tcgaggtcgg
      361 atcgagaccg gcggcgttga acggcggtac ataggcgccc gccgcggcat gccagaaacc
      421 atgcaacggc gcgagcgggt cggccgcgct tggcgcgctg aggtagtagg gaaccggcag
      481 tacgccgata tagatgtcgg cgaccggcgg caggccgacg ccgagtttgg aaagatccag
      541 gccggtcggg gccagggtcg cctgcgctgc cggcgtctgt tcgacgcggc cctgcacggc
      601 ctgcatcacc ggcgtgatcg cctgcgtggt tgcgacccag gacagcacga tgcgttggtc
      661 cgtggctgct ccgtcgaact ggtcgactgc gtattcctgc gaattgacga gctgacgcag
      721 cggttcgagt gcgcaggcct gcgaggccgg cagcagcgga tcggtggact gcccgttcac
      781 gcacagcgcg ctggtgcgct tggcgaggaa atacgtcgtg tcgggcgtta cgtcgttgcc
      841 ggtaccgtcg gtgatgctcg gcgacggcgc gggattgagc tggccgccgt tgccgatcag
      901 cacgaccatg taggaagtca attgttgcag cggacgcgtt ggcacgatgg ccagggtgcg
      961 gccgctggaa tccgatggcg aaatcagggc cacgaaatcc tgcggcgcct tgagttcgcg
     1021 ggtgatgccg gtgacgccgc caccgggtcc ggtcaggctc acctggaaga cgcgcaccgt
     1081 gccgccggga ccggggatga tggtcgaagg tttgggcgcg gtgctgaacg tggtgctcca
     1141 tggtgccgtg gtggagaatc cgtccagtgc gttcaatgcg acctgcggat cgctgagatt
     1201 ggcgggatcc gcgaccggaa tgttcagagt cagatccgtg gttcccgaca acagcaggtt
     1261 ggtcggaaac ggcactaccg cattggtcgg atcgaagcgt gcggtgatca ccgcggtcac
     1321 cggcgaaccg ccgctattgt tcgcgcccga atcggatcca tgcggggcgt tgctgccgcc
     1381 tccgcaggcc gacagcagaa gcgatgagga aagggcgagg agcaaactca gtcgggtctg
     1441 catgacaaaa tcctgatcag tggtgggagc gcaacggcag gcctatcata ggcatttcaa
     1501 acgagcgttg gcaacgccaa cttaacggtt tcattatgac tttcgctgct gcaatgcagc
     1561 agatattggg gtcgtgctga catcgacgta tacatctata cccacgtcat tgc
//
LOCUS       DASRVO010000797         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_206178, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000797 DASRVO010000000
VERSION     DASRVO010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206178"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VFF05_09855"
     CDS             complement(<1..687)
                     /locus_tag="VFF05_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HZW52157.1"
                     /translation="MIQATRGAGPRTGDDSPPLQALANRSDWPSRVRQLIEGVLGMCT
                     TSLERAVVAALDDTESQLFKLAEQSRNNESQYRCFETLREIKRGRADVAPRLMLAVED
                     KLARFDRNGAMQAVNAATRPAMPVRKELALVETRELEESLALQEFVAKTEVRQAPALF
                     LLGHRFGVLAAAPAFDAETLAVGPAGLAEALQHACACLDISAEHRVLLFHAFDRSSHA
                     AIGDLYAALNH"
     gene            complement(869..>2371)
                     /locus_tag="VFF05_09860"
     CDS             complement(869..>2371)
                     /locus_tag="VFF05_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZW52158.1"
                     /translation="AFAWENLPLDSVERIEIVRGPRASYWGSDAIGGVIQIFTRKLTG
                     PHLAASYGSYRSADGSTGFGARDGSGGFSVQVGARHVGGFSATNPGICNGPDDPYCIY
                     NPDANGAQYHGGTLSGDYKLGTQTLSASLLRSEGTQNFDNGASDGISHTLDQSIGAAL
                     EGDVTTAWHQRLALGTAREDISTPAFAAAYRSTREQAAWTNDVTVSGAQHFVAGFDAA
                     HEHGVSIDLSGFGAPYERARDMTGAFAGWRVSDGAFDSELAGRYDHNSQFGGAFSGSA
                     AGAWKFSDALRLTASFGTAFRAPDLNELYSPGYGGYYAGNPDLKAERSRTLELGAEWT
                     PDANNRLAVHAISTRVHDLIDFAGGSTYQAVNIDRAAIDGVELTQNVRIDVWSLTNNV
                     TVQNVRDADTGAQLLRRPKRKLSSLLERGFGERLSTGMEFIVVGPRQDVGGLVLPGYA
                     MANLRADYRLNAAWRVGARLENLFNRSYELAQGYNTPGRSAYLTLTWSPR"
BASE COUNT          389 a          735 c          810 g          437 t
ORIGIN      
        1 atgattgagc gccgcgtaaa gatcgccgat ggcggcgtgg ctggagcggt cgaaggcgtg
       61 gaacagcagc acgcgatgct cggcgctgat atccaggcat gcgcaggcgt gttgcaatgc
      121 ctcggccagc ccggccggac cgaccgccag tgtttccgca tcgaaggccg gggccgcggc
      181 gagcacgccg aagcgatggc cgagcaggaa caaggcgggt gcctggcgga cttcggtctt
      241 ggcgacgaat tcctgcaaag ccagagattc ttccagttcg cgcgtttcca ccagcgccag
      301 ttccttgcgc acgggcatcg cgggacgcgt cgcggcattc acggcttgca tggcgccgtt
      361 gcggtcgaag cgggcgagtt tgtcctcgac ggccagcatc aggcgtgggg cgacgtcggc
      421 gcggccgcgc ttgatttcgc gcagggtttc gaagcagcgg tattgcgact cgttgttgcg
      481 gctctgttcg gcgagcttga acaactggga ttcggtgtca tccagtgccg ccaccaccgc
      541 acgttccagg ctggtggtgc acatgccgag cacgccctcg atcagttgac gcacccgcga
      601 aggccagtcg ctgcggttgg cgagcgcttg cagtggtggc gagtcgtcgc cggttctggg
      661 tcccgctccg cgggtggcct gaatcatgtt tcgtccccgt ccgctgcccg cggcatctgc
      721 cgcgggcctg tcgtcatgtc atttcgagtg gtcatagcct cgcatgcttc ggccatccag
      781 gcatttatgc gaattttatg ccgacacggt agcagcagaa atggaaattt gtccagacat
      841 acgtgacccg ccggaccggg cggaaatcct agcgtgggct ccaggtcagc gtgaggtagg
      901 ccgaccgtcc cggcgtgttg tagccttggg ccagctcata ggaacgattg aacaggtttt
      961 ccagtcgcgc gccgacgcgc caggccgcat tcagccggta atcggcgcgc aggttggcca
     1021 tggcgtaacc cggcagcacg agtccgccga cgtcctgccg cgggccgacg acaatgaatt
     1081 ccatgccggt cgacaggcgc tcgccgaagc cgcgttcgag caggctggaa agcttccgct
     1141 tcggccgccg cagcaattgc gcgccggtat ccgcatcgcg cacattctgg acagtgacat
     1201 tatttgtcag cgaccagacg tctatgcgta cgttttgagt cagttcgacg ccgtcgatgg
     1261 cggcgcggtc gatgttgacg gcctgataag tgctgccgcc ggcgaaatcg atcaggtcgt
     1321 ggacgcgtgt ggaaatagca tgcacggcca gacgattgtt cgcatccggc gtccattcgg
     1381 cgccgagttc gagcgtgcgc gaacgctccg ccttcaaatc gggattgccg gcgtaatagc
     1441 cgccatagcc cggcgaataa agctcgttga gatcgggtgc gcggaaggcg gtgccgaaac
     1501 tcgcggtcag gcgcagtgcg tcggagaatt tccatgcgcc cgccgccgaa ccggaaaacg
     1561 caccgccgaa ctggctgttg tggtcgtagc gcccggccaa ctcgctgtcg aaggcgccgt
     1621 cggaaacgcg ccaaccggcg aaggcgccgg tcatgtcgcg ggctctttcg taaggtgcgc
     1681 cgaatccgga caggtcgatg ctcacgccgt gttcgtgcgc ggcgtcgaag ccggcgacga
     1741 agtgctgtgc gccggacacg gtcacgtcgt tcgtccatgc cgcctgttcg cgcgtggatc
     1801 ggtaggctgc ggcgaacgcg ggcgtggaaa tgtcctcgcg cgccgtgccg agcgcgaggc
     1861 gctgatgcca ggcagtggtc acatcgcctt ccagcgccgc gcctatggac tggtcgagcg
     1921 tgtgcgaaat gccgtcgctc gcgccgttgt cgaaattctg cgtgccttcg ctgcgcaaca
     1981 ggcttgccga gagagtttgc gtgccgagct tgtaatcgcc gctgagcgtg ccgccgtggt
     2041 attgcgcgcc gttggcgtcc gggttgtaga tgcagtaggg gtcgtccggg ccgttgcaga
     2101 tgccgggatt cgtcgcggaa aaaccgccga cgtggcgcgc gccgacctgc acgctgaaac
     2161 cgccggagcc gtcgcgcgcg ccgaatccgg tactgccgtc ggcactgcga tagctgccgt
     2221 aacttgcggc cagatgcggg ccggtcaact tgcgcgtgaa aatctggatc acgccgccga
     2281 tcgcatccga gccccagtag ctcgcgcgcg ggccgcgcac gatctcgatg cgttcgacgg
     2341 aatccagcgg caggttttcc caggcgaacg c
//
LOCUS       DASRVO010000798         1007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_50620, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000798 DASRVO010000000
VERSION     DASRVO010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50620"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1007
                     /gene="hemN"
                     /locus_tag="VFF05_09865"
     CDS             <1..>1007
                     /gene="hemN"
                     /locus_tag="VFF05_09865"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HZW52159.1"
                     /translation="YPAAPQFHAGFGEAQLRAAAHASNEDPIPRPLSLYVHVPFCLSP
                     CFYCGCTRIITRDKGRTPIYMQRLFREIELTAPLFDRDRSVLQLHFGGGTPNFLDAGQ
                     MSELLETLAQHFSFSRDPRREFGIELDPRFCDAAYVHALAPLGFNRVSVGIQDFDPEV
                     QAAVNRIQSVAQTRAVLDAARESGFRSTSVDLIYGLPKQTLAGFARTLDEVIALQPDR
                     VAAYAYAHLPKLFKAQNQIHAADLPDTATRLRLLGLTVEKLTDAGYRYIGMDHFARAD
                     DELARALEAGTLQRNFQGYSTFGGCDLIGLGMSAISHVGASFSQNARDLPNYYAALDA
                     G"
BASE COUNT          169 a          371 c          294 g          173 t
ORIGIN      
        1 ctacccggcc gcgccgcagt tccatgccgg gttcggcgag gcgcaactgc gtgccgccgc
       61 gcacgcctcc aacgaggatc cgattccgcg gccgttgtcg ctgtatgtgc acgttccgtt
      121 ttgcctgagt ccctgcttct attgcggctg cacgcgcatc atcacgcgcg acaagggcag
      181 gacgccgatc tacatgcaac gcctgtttcg cgagatcgaa ctgaccgcgc cgctgttcga
      241 ccgcgaccgc agcgtgctgc aactgcattt cggcggcggc acgccgaact ttctcgacgc
      301 gggccagatg agcgaattgc tggagaccct ggcccagcat ttttccttca gccgcgaccc
      361 gaggcgcgaa ttcggcatcg aactcgaccc gcgcttctgc gatgcggcgt atgtgcacgc
      421 actggcgccg ctcggtttca accgcgtttc ggtcggcatc caggatttcg atcctgaagt
      481 gcaggccgcg gtcaaccgca tccagagcgt tgcgcagacc cgcgcagtgc tcgacgcggc
      541 gcgcgaatcg ggcttccgct ccaccagcgt cgacctgatc tacggcctgc caaaacagac
      601 actcgccggt ttcgcgcgca cgctcgacga ggtgatcgcg ctgcaacccg accgcgtcgc
      661 ggcctacgcc tacgcgcacc tacccaagtt gttcaaggcg cagaaccaga tccacgccgc
      721 cgatctgccg gacaccgcca cacgcctgcg cctgctcggc ctgaccgtgg aaaaactcac
      781 cgacgccggc taccgctaca tcggcatgga tcacttcgcc cgcgccgacg acgagctcgc
      841 gcgtgcgctc gaagccggca ccctgcaacg caatttccag ggttattcca ccttcggcgg
      901 ctgcgacctg atcggcctcg gcatgagcgc gatctcgcac gtcggcgcga gcttcagcca
      961 gaacgcgcgc gacctgccca actactacgc tgcgctcgat gcgggcc
//
LOCUS       DASRVO010000799         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_75836, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000799 DASRVO010000000
VERSION     DASRVO010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75836"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..752)
                     /locus_tag="VFF05_09870"
     CDS             complement(147..752)
                     /locus_tag="VFF05_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HZW52160.1"
                     /translation="MTALADVDLVLASTSRYRRELLARLAPDFRCVAPDADENPLPDE
                     SPAALADRLALAKAQAVAAKNPGAIVIGSDQVAHVDGDNRVVGKPHTPEIARAQLAGF
                     SGRTVLFRTSVCVVDARHSPHREFAVQDTTRVKFRTLDAGAIARYVERERPLDCAGSF
                     KCEGLGIALFERIQSEDPTALIGLPLIALCRLLRELEIEIP"
     gene            complement(749..1243)
                     /locus_tag="VFF05_09875"
     CDS             complement(749..1243)
                     /locus_tag="VFF05_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52161.1"
                     /translation="MGKLHARNGEIRIESTLVIVAVCLVLAVLVLWLRDRGERGRQNA
                     IRALLDSADLLESRLQECRERTQHLRDMLIVLPEEMSARADSALQTDAKVQAALKDLL
                     AHRLWIQQHAATASPRELAQAQKALDQSGATLQSQLDRLAAITADLQRAQTESRTLQK
                     PKSA"
     gene            1324..1839
                     /locus_tag="VFF05_09880"
     CDS             1324..1839
                     /locus_tag="VFF05_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014402827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HZW52162.1"
                     /translation="MNNVLPESVDAWRMVQARRSFQGSLPLASLPRLRGSLAATDGAV
                     EFDLEFGKDDLGVAHLRVRADASLPLTCQRTLDVFRLPVHVDTRLGLIAREEDEAGLP
                     GDYEPLLTADGALHLADVVEDELILALPAVPVKPGTEYKEPEFAGAVQDDAKEPGNPF
                     AVLQNLKHKKT"
     gene            1856..2050
                     /gene="rpmF"
                     /locus_tag="VFF05_09885"
     CDS             1856..2050
                     /gene="rpmF"
                     /locus_tag="VFF05_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZW52163.1"
                     /translation="MAVAKSRKSPSRRGMRRAHDALSAKQLGTDPTSGEVHVRHNVTK
                     DGYYRGRKVIADKVKVKDED"
     gene            2075..>2968
                     /locus_tag="VFF05_09890"
     CDS             2075..>2968
                     /locus_tag="VFF05_09890"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZW52164.1"
                     /translation="MALIYARIAGTGSFLPEKVLTNADLEKLVDTSDEWIASRTGIRQ
                     RHIAAEGQTTCDLAEGAARAALDAAGVKASEIELLIVGTTTPDLIFPSTACLLQHRLG
                     ANGCAAFDVNAACSGFIYALSIADKFIRSGAVKTALVVGAETLSRMLDWSDRGTCVLF
                     GDGAGAVVLKAADEPGILSTHIHADGGYKELLWNPVGVSVGFKPGEHNAGVKVLMAGS
                     EVFKVAVKTLDRVVDETLAANGLDKHDIDWLIPHQANLRIIQATAKRLDMPMDRVVVT
                     VDKHGNTSSGSVPLALDYAVRT"
BASE COUNT          516 a          944 c         1003 g          505 t
ORIGIN      
        1 cctgtgctcg cgcgtgggca atctcgcgcc cgccgcgcgg ctcgacagtt acgcgggccg
       61 caagcgctat cgctggtacg ctggcgcggt gacagaatca gaaaacgtcg aatcgaatcc
      121 ggattgcgtc gccgccaaac cgtgattcac gggatttcga tttccaactc gcgcagcagc
      181 cggcacagcg cgatcagcgg cagtccgatc aatgcggtgg gatcttccga ttggatgcgc
      241 tcgaacaggg cgatgccgag tccttcgcat ttgaaactgc cggcgcaatc cagcgggcgt
      301 tcgcgttcga cgtagcgggc gatcgcgccc gcatcgaggg tgcggaattt cacgcgcgta
      361 gtgtcctgca cggcgaattc gcgatgcggc gaatggcgcg catcgaccac gcagaccgat
      421 gtgcggaaca gcacggtgcg cccggaaaag ccggcgagct gggcgcgggc gatttcgggg
      481 gtgtgcggct tgccgacgac gcgattgtcg ccgtcgacat gcgcgacttg atcggagcct
      541 atgacgatgg cgccgggatt tttcgcagcc acggcctgcg ccttggccag ggcgaggcgg
      601 tcggcgagcg cagcgggact ttcatccggc agcggattct cgtcggcatc cggcgccacg
      661 cagcgaaaat ccggtgccag ccgcgccaac agttcgcgcc ggtagcgcga agtcgaagcc
      721 agaaccagat cgacgtcggc cagcgcggtc atgccgattt cggtttttgc agggtgcgcg
      781 attcggtctg cgcgcgctgc aagtccgccg tgatggcggc gaggcggtcg agttgggact
      841 gcaaggtggc tccggactga tccagcgctt tttgcgcctg cgccaattcg cgcggcgagg
      901 cggtcgcggc gtgttgctgg atccacaggc ggtgcgcgag cagatccttg agcgcggcct
      961 gcaccttggc gtcggtctgc agggcgctgt cggcgcgtgc gctcatttcc tcgggcagga
     1021 cgatgagcat gtcgcgcaag tgctgcgtgc gttcgcggca ttcctgcagg cgcgattcca
     1081 gcaggtcggc gctgtcgagc agggcacgaa tggcgttctg gcggccgcgt tcgccgcggt
     1141 cgcgcagcca cagcaccaac acggcaagaa ccaggcagac ggcgacgatg acgagagtgg
     1201 attcgatgcg gatttccccg ttgcgagcgt gcagtttgcc caaaccatgg cgtcgggtaa
     1261 accttcgact ctgaacggat tcggttgaca ttgtgcagtg cggcacctac catcccgcgc
     1321 gccatgaaca acgtgttacc ggaatccgtg gacgcgtggc gaatggtgca ggcgcgacgg
     1381 tcgtttcagg gcagtctgcc cctcgcttcg ttgccgcggc tgcgcggcag cctggcggcc
     1441 accgatggcg ccgtggaatt cgatctggaa ttcggcaagg acgatctggg cgtggcgcat
     1501 ctgcgcgtgc gcgcggatgc gtcgctgccg ctgacctgcc agcgcacgct ggacgttttc
     1561 cggctgccgg ttcatgtgga cacgcgcctg gggctgattg cgcgggaaga agacgaggcg
     1621 gggttgcccg gcgactacga gccgctgctc acggccgacg gcgcactgca cctggccgac
     1681 gtggtcgaag acgagttgat cctggccttg ccggcggtgc cggtgaagcc cggaacggaa
     1741 tacaaggagc cggaatttgc cggcgccgtg caggacgatg cgaaggaacc cggaaatccc
     1801 ttcgccgtgc tgcagaacct caagcacaag aaaacctgat ctggagtaat gcgtcatggc
     1861 cgttgccaaa agccgtaaat ccccgtcccg ccgcggcatg cgccgcgccc acgatgcgct
     1921 gagcgccaag cagctcggca ccgacccgac ctcgggcgaa gtgcacgtgc gccacaacgt
     1981 caccaaggac ggctactacc gtggccgcaa ggtcatcgcc gacaaggtca aggtgaaaga
     2041 cgaggattga ttcctcaccg cacgctgcag gggaatcgct ctgatttacg ctcgcatcgc
     2101 cggtaccggc agtttcctgc cggaaaaggt gctcaccaac gccgatctgg aaaagctcgt
     2161 cgacacctcc gacgagtgga tcgcgtcgcg cacgggtatc cgccagcgcc acatcgccgc
     2221 cgaaggccag accacctgcg atctggccga gggtgcggcg cgcgcggcgc tggacgcggc
     2281 cggcgtcaag gccagcgaga tcgagctgct gatcgtcggc acgaccacgc cggatctgat
     2341 ctttccctcg acggcctgtc tgctgcaaca tcgcctcggc gcgaacggct gcgccgcatt
     2401 cgacgtcaac gccgcgtgct cgggcttcat ctacgcgctg tccattgccg acaagttcat
     2461 ccgctccggc gcggtcaaga ccgcactcgt ggttggcgcg gaaacgctct cgcgcatgct
     2521 cgactggtcc gatcgcggca cctgcgtgct gttcggcgac ggtgccggag cggtcgtgct
     2581 caaggctgcg gacgagcctg gcatcctgtc cacgcacatc cacgccgacg gcggatacaa
     2641 ggaattgctg tggaacccgg tcggcgtctc ggttggtttc aagcccggcg agcacaatgc
     2701 cggcgtgaag gtgctgatgg ccggcagcga agtgttcaag gtcgcggtga agacgctcga
     2761 ccgcgtcgtc gatgaaaccc tggccgcgaa cggcctggac aagcacgaca tcgactggct
     2821 gattccgcac caggccaatc tgcgcatcat ccaggccacg gccaagcgcc ttgatatgcc
     2881 gatggatcgc gtcgtcgtca ccgtcgacaa acacggcaat acttcgtccg gctcggtgcc
     2941 gctggcgctc gattacgcgg tgcgcacc
//
LOCUS       DASRVO010000800         1444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_310345, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000800 DASRVO010000000
VERSION     DASRVO010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310345"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFF05_09895"
     CDS             complement(<1..881)
                     /locus_tag="VFF05_09895"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HZW52165.1"
                     /translation="MAAITLIEAVTQALAYEMKHDPSVVVLGEDVGVNGGVFRATQGL
                     SEKFGEWRVFDTPLDETTIAGVTVGLAAQGMKPIAEAQFEGFIYPMMEQIVCHAARMR
                     NRTRGRLTCPAVFRAPWGGGIHAPEHHSEANEHLFTNVPGLRVVLPSSPARAYGLLLA
                     AIRDPDPVIFFEPKRIYRQYKEEVPDDGEALPLDVCFVLRDGTDITLVTWGAQVKESL
                     EAAEQLAKEGISAEVIDLATLTPLDFDTIAESVQKTGRCVIVHEAPRTAGFGAEIAAR
                     IAEECIYDLLAPVVRVTG"
     gene            complement(883..>1444)
                     /locus_tag="VFF05_09900"
     CDS             complement(883..>1444)
                     /locus_tag="VFF05_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZW52166.1"
                     /translation="AINVAGARELPLVAAIVNNQWAISVPRKIQSGAKTLAQKGIAAG
                     LACIQVDGNDIIAVKKAMGDAIERARKGDGGSVVECVTYRLGDHTTADDARRYRGKEE
                     VEDAWKKDPVKRLRAWLDAQKAWDQKREDAWQAECGEWMDNEVNTYLETKAQPVTAMF
                     DYTFAELPADLAKQREEAIALEKGAH"
BASE COUNT          207 a          473 c          475 g          289 t
ORIGIN      
        1 ccggtcacgc gcacgaccgg tgcgagcaaa tcgtaaatgc attcttcggc gatgcgtgcc
       61 gcgatctcgg caccgaaacc ggcggtcctg ggcgcttcgt gcacgatcac gcagcggccg
      121 gttttttgca cggattcggc gatcgtgtcg aaatccagcg gcgtcagcgt ggcgaggtcg
      181 atgacttcgg cgctgatgcc ttccttcgcg agttgctcgg cggcttcgag cgactccttg
      241 acctgcgcac cccaggtcac cagggtgatg tcggtgccat cgcgcagcac gaagcacacg
      301 tccagcggca gcgcctcgcc gtcgtcgggc acttcttcct tgtactggcg gtagatgcgc
      361 ttgggctcga agaagatcac cgggtccgga tcgcggatcg cggcgaggag caagccatac
      421 gcgcgtgcgg gcgaggacgg cagcaccacg cgcagtcccg gcacgttggt gaacagatgc
      481 tcgttcgctt ccgaatgatg ctcgggcgcg tggatgccgc cgccccacgg cgcgcgaaaa
      541 acggccgggc aggtgaggcg tccgcgcgtg cggttgcgca tgcgcgcggc gtggcagacg
      601 atctgctcca tcattggata gatgaagcct tcgaactggg cctcggcgat cggtttcatg
      661 ccctgcgcgg cgaggcccac ggtgacgccg gcgatggtgg tttcgtccag cggcgtgtcg
      721 aacacgcgcc attcgccgaa tttttccgaa agaccctgcg tggcgcggaa cacgccgccg
      781 ttgacgccga cgtcctcgcc gagcacgacc accgatgggt cgtgtttcat ttcgtaggcg
      841 agcgcctggg tcaccgcttc gatgagggtt atcgcagcca tgtcagtgcg ctcctttctc
      901 gagcgcgatc gcttcttcgc gttgtttggc gagatcggcc ggcaactccg cgaacgtgta
      961 gtcgaacatc gcggtgacag gctgcgcctt ggtttcgagg taggtgttca cctcgttgtc
     1021 catccactcg ccgcactcgg cctgccaggc gtcttcgcgt ttctggtccc aggccttctg
     1081 cgcatcgagc caggcccgca ggcgcttgac cgggtccttc ttccaggcat cttcgacttc
     1141 ttccttgccg cggtagcggc gcgcatcgtc ggcggtggtg tggtcgccca ggcgatacgt
     1201 cacgcattcc acgacgctgc cgccgtcgcc cttgcgcgcg cgctcgatgg catcgcccat
     1261 cgctttcttc accgcaatga tgtcgttgcc gtcgacctgg atgcaggcca gtcccgccgc
     1321 gatgcctttc tgcgccagtg tcttggcgcc ggactggatc ttgcgcggca ccgagatcgc
     1381 ccactggttg ttgacgatgg cggcgaccag cggcagctcg cgcgcgccgg cgacgttgat
     1441 cgcg
//
LOCUS       DASRVO010000801         1166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_360510, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000801 DASRVO010000000
VERSION     DASRVO010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360510"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFF05_09905"
     CDS             <1..646
                     /locus_tag="VFF05_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HZW52167.1"
                     /translation="YAGDVPANIGSTLSMAFNGTADQYQNASGVASTLTDNFGIEAWV
                     KSNGSTSGNAAIAYNGNSSNSGWGIYRFGGSYGLLYGGNIAANASPISTAWTELALVR
                     DNGTTTFYVNGVASSTTTTGPNLPTGSVAIGGNPLGSLSPPPQGVPQEFFGGEIDEVR
                     IFSFAPGAFAVGDLNLPPPPPLVPTPVLSLRSLGLLALGLFGLAWLGLRRRISG"
     gene            complement(636..>1166)
                     /locus_tag="VFF05_09910"
     CDS             complement(636..>1166)
                     /locus_tag="VFF05_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HZW52168.1"
                     /translation="EIIEPTLAGMAAEGAPFIGFLYAGLMIDRHGAPRVIEFNVRLGD
                     PETQPILLRLKSDLVDMIEAAIDGRLDKLSISWDERVALGVVLAAHGYPGKVRLGDAI
                     EGLDADFGADVKVFHAGTKRENGKIVTAGGRVLTVCALGEDFAEAQTRAYAAVRKIKA
                     DGLFCRRDIGWRAISR"
BASE COUNT          203 a          382 c          364 g          217 t
ORIGIN      
        1 ctacgcaggg gacgtgccgg cgaatatcgg cagcaccttg tcgatggcgt tcaacggcac
       61 tgccgaccag tatcagaatg cttccggggt tgcctcgacg ctgaccgaca atttcggcat
      121 cgaagcatgg gtgaaatcca acggcagcac cagcggcaac gccgcaattg cttacaacgg
      181 caactccagc aattcgggtt ggggaatcta ccggttcggc ggcagttacg gtctgctcta
      241 cggcggcaat atcgcggcca acgccagtcc catttccacg gcatggaccg aactggctct
      301 agtgcgcgac aacggcacga ctacgttcta tgtgaatggt gtcgccagct ccaccacgac
      361 gacagggccg aatctgccga cggggagcgt ggccatcggc gggaatccgc taggctcgtt
      421 gtccccgcca ccgcaggggg ttccacagga attcttcgga ggcgaaattg acgaagtacg
      481 catcttcagt ttcgcgcccg gcgcgttcgc tgtcggcgac ttgaacctgc cgccaccacc
      541 gcctctggtg ccgacgccgg tgttgtcgtt gcgttcgctg ggcctgctgg cactgggact
      601 tttcggattg gcctggctcg gcctgcgccg ccgcatcagc ggctgatcgc gcgccagccg
      661 atatcgcggc ggcagaacag gccgtcggcc ttgatcttgc gcactgccgc gtaggcacgt
      721 gtttgtgcct cggcgaaatc ctcgccgagc gcgcacacgg tcagcacgcg gccgccggcg
      781 gtgacgatct tcccgttttc ccgtttcgtg ccggcgtgga agactttcac gtcggcgccg
      841 aagtccgcat ccagtccctc gatcgcatcg cccaggcgca ccttgccggg ataaccgtgc
      901 gcagcgagta ccacgccgag cgcgacacgc tcgtcccagg aaatgctcaa tttgtcgagg
      961 cgtccgtcaa tggcagcctc gatcatgtcc accaggtcgg acttcaggcg cagcaggatc
     1021 ggctgggttt ccgggtcgcc cagacgcacg ttgaactcga taacgcgcgg cgcgccgtgc
     1081 ctgtcgatca tgaggcccgc gtacaagaag ccgatgaaag gcgcgccttc ggccgccatg
     1141 ccggccaggg tcggctcgat gatttc
//
LOCUS       DASRVO010000802         1064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_310466, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000802 DASRVO010000000
VERSION     DASRVO010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310466"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VFF05_09915"
     CDS             complement(<1..619)
                     /locus_tag="VFF05_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZW52169.1"
                     /translation="MALDFAEKSAARQWVWDRLAAQGVARFPFPPHGRIPNFAGAEVA
                     AVRLFEIEPWKSASAIKVNPDSPQRPLRAEALRRGITVFVPTPRLRGGFKKLDPDRIP
                     PDRVKEAAGLSRGDRWSEEVALAAMPRLDAIVCGAVAVTRDGRRCGKGEGYSDLEFAI
                     LRELGHPPVPVATTVHDLQIVGSVPRDPTDLPLSVIATPTRAIRVK"
     gene            628..>1064
                     /locus_tag="VFF05_09920"
     CDS             628..>1064
                     /locus_tag="VFF05_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52170.1"
                     /translation="MEQRSLSTVKNAIAASRGAAEPDAVAALLKAGADPGHLLGFVVE
                     HKNERLLRVMMQAGVEMNHLEADRWDPRFFSLAKWPAGLALLLEHDANPETEGRTGFT
                     VLMLAIHMEYWPSVDLLLAHGARTDDGAHRLSLRKLVSEKIEQ"
BASE COUNT          180 a          344 c          361 g          179 t
ORIGIN      
        1 gcttgacgcg gatcgcgcgg gttggcgtcg cgatcacgct gaggggtaga tcggtggggt
       61 cgcggggcac ggaaccgacg atctgcagat cgtgcacggt cgtggcgacc ggcaccggcg
      121 ggtgaccgag ttcgcgcagg atggcgaatt ccaggtcgct gtagccttcg cccttgccgc
      181 agcgccgccc gtcgcgcgtc accgcgacgg cgccgcagac aatggcatcc agccgcggca
      241 tggccgcgag cgccacctct tccgaccagc ggtcgcctcg cgacaggcct gccgcttctt
      301 tgaccctgtc gggtggaatc cggtccggat caagcttctt gaagccgccg cgcaatcggg
      361 gcgtgggcac gaaaaccgtg atgccgcggc gcagcgcctc ggcccgcagc gggcgttgcg
      421 gcgaatccgg attgaccttg atggcgctgg cgctcttcca cggctcaatc tcgaataggc
      481 gcactgcggc cacttcggca cccgcgaaat tcgggatgcg gccatgcggc ggaaacggaa
      541 agcgtgccac gccttgcgcg gcaagcctat cccagaccca ctgccgcgca gcgctttttt
      601 cggcgaagtc cagggccatg ccgcaaagtg gagcagcgct cgctgtcgac agtcaagaac
      661 gcgatcgcgg catcgagagg ggccgcggaa cctgacgccg tcgccgctct gctcaaggcc
      721 ggcgccgatc ctggccattt gctcgggttc gtggtcgaac ataaaaacga acgcctgctg
      781 cgggtgatga tgcaggccgg cgtcgagatg aaccacctcg aagccgaccg gtgggatccg
      841 cgtttcttct cactggcgaa gtggcccgca ggtcttgctt tgttgctgga gcacgacgcg
      901 aacccggaga cggagggccg caccggcttt accgtcctta tgctggcgat ccatatggaa
      961 tattggccga gcgtcgatct cctgctggcc catggggcgc gtaccgacga tggcgcccat
     1021 cggctaagcc tgcgcaagct cgtatcggag aagatcgagc agat
//
LOCUS       DASRVO010000803         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_387206, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000803 DASRVO010000000
VERSION     DASRVO010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387206"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VFF05_09925"
     CDS             <1..1254
                     /locus_tag="VFF05_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZW52171.1"
                     /translation="ESAKIPGLGITRLQAAMDDQLLRTGIELHRHGMDGLRQWLQSSE
                     GYGPGTIYVVDAQDHDLLGRTLPDAARAALTAAPASEPPERVRIHPIHMRDDPVEYRA
                     VAVFGGSFFTRLIWRRPMTFWSNIGIAMLICALASALLASYVAAPLSRIRASARRVAR
                     GDLSARVGEVRFGRSAEVLALAKEFDGMAARLKELVDGQRRLIRDVSHEMRSPLSRLR
                     VALELARPQASGDAATQLDRIERESERIEEMIAQAIQLSRMETTLPEQVDDVAFDELV
                     ADIAADAAFEAQARPCTLRIAQSAPLTVRAEGELLASAVENVVRNAVHYTAPHSEVDL
                     RLDRVEGQARLRVRDTGPGVAAADCARIFEPYFRTDAARQRKSGGSGLGLAIAKRAIE
                     RQGGRIRASNADGGGLEIEILLPLA"
     gene            complement(1251..1883)
                     /locus_tag="VFF05_09930"
     CDS             complement(1251..1883)
                     /locus_tag="VFF05_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52172.1"
                     /translation="MWGGVQLTYGIRRRRISMKRLSILIVLLLVACGRSTPQQQTAHA
                     RALTPGASSGVPGGVLINPDFEQTATDGSIPGWNTAQHAGQPAYVMWIDAYKPYAGHG
                     SFHVTRTQPQVYGSLTQTLDARAYAGKTVELSAMLKALGVGPGGWKLIISANMPGTLA
                     YSPELTGDSAWTRESVRLSVPAKAQRLTVGVSLRDGGDGWMDNVELKVVD"
     gene            1907..2863
                     /gene="hprK"
                     /locus_tag="VFF05_09935"
     CDS             1907..2863
                     /gene="hprK"
                     /locus_tag="VFF05_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HZW52173.1"
                     /translation="MERLTARELFDKISQRLSLRWVAGQRGESRAIVPAEKQPRRPSL
                     AGYLNIIYPNKIQIVGSEELSWLDSLDSRLRWEMVEKIMGFSPTALIVTKDQAVPADL
                     RDAANESGTPMWVSSQRGHELLTYLQYHLARMLARRVTLHGVLMEVYSIGVLITGESG
                     SGKSELALELITRGHRLVADDAPEFIQIAPDVIDGTCPEMLQDLLEVRGLGILNVREM
                     FGQTAVKPSKYLRLIVHLDPLPREQPIEQGGLERIYGANSYREILDTKIPQATIPVAP
                     GRNLAVLVEAAVRNHVLKTKGIDPAQTFLDRQAHQMQRQAPW"
     gene            complement(2955..>3193)
                     /locus_tag="VFF05_09940"
     CDS             complement(2955..>3193)
                     /locus_tag="VFF05_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52174.1"
                     /translation="LAKDTVVLNTSVKINDTTVLKGKKPEVVASGICRYRSPETKDGP
                     KVDDLLANNGDKLKQTLSAALDRCVQDYRDKLFL"
BASE COUNT          585 a         1102 c          979 g          527 t
ORIGIN      
        1 gaaagcgcga agattcccgg ccttggcatc acgcgcctgc aggcggcgat ggacgaccag
       61 ctcctgcgca ccggcatcga actgcaccgc cacggcatgg acggcctgcg ccaatggctg
      121 cagagctccg aaggttatgg accgggaacg atctacgtcg tggatgcgca agaccacgac
      181 ctgctcggcc gcacgctgcc cgacgcggcg cgcgcggcac tgacggccgc gcccgcatcg
      241 gagccgcccg aacgcgtgcg catccatccc attcacatgc gcgacgatcc cgtggaatac
      301 cgcgccgtgg ccgtgttcgg cggttcgttt ttcacgcgcc tgatctggcg caggccgatg
      361 acgttctggt ccaacatcgg catcgccatg ctcatttgcg ccctggccag cgccctgctc
      421 gcgtcctatg tggccgcgcc gctctcgcgc atccgcgcca gcgcgcgccg cgtcgcgcgc
      481 ggggatttgt cggcgcgcgt cggcgaagtg cgcttcggcc gcagcgccga ggtgctcgcg
      541 ttggcaaaag aattcgacgg catggccgcg cgcctgaagg aactggtcga cggccagcgc
      601 cgcctgatcc gcgatgtctc gcacgagatg cgctcgccgc tgtcgcgcct gcgcgtggcg
      661 ctggaactgg cgcgaccgca agccagcggc gatgccgcca cgcagctcga ccgcatcgag
      721 cgcgagtccg agcggatcga ggaaatgatc gcgcaggcga tccagctctc gcgcatggaa
      781 accacgctgc ccgagcaggt ggacgacgtc gccttcgacg aactcgtcgc cgacattgcc
      841 gccgacgccg cattcgaggc gcaggcgcgc ccgtgcacgc tgcgcatcgc gcaaagcgcg
      901 ccgctgaccg tgcgcgcgga aggcgaactg ctcgccagcg cggtggaaaa cgtggtgcgc
      961 aacgccgtgc actacaccgc gccgcacagc gaagtggacc tgcgcctgga ccgcgtcgaa
     1021 ggtcaggcgc ggctgcgcgt gcgcgacacc ggccccggcg tggcggccgc cgattgcgca
     1081 cgcatcttcg aaccgtattt ccgcaccgac gccgcacgcc agcgcaagag cggcggcagc
     1141 ggcctcggcc ttgccatcgc caagcgcgcg atcgaacgcc agggcgggcg catccgcgcc
     1201 agcaatgcgg atggcggcgg cttggagatc gagattctac ttcccctggc ttagtcgacg
     1261 accttcagct cgacgttgtc catccagcca tcgccaccgt cgcgcagact gactccgaca
     1321 gtcagtcgtt gcgctttcgc gggcacgctc aaacgtacag attcccttgt ccaagcacta
     1381 tcgccagtca gctccggcga ataagccagc gtgcccggca tgttggcgct gatgatcaac
     1441 ttccagccgc caggcccgac acccagtgcc ttgagcattg cggatagctc gaccgtcttg
     1501 cccgcatagg cgcgcgcatc gagcgtttgc gtcaacgaac cgtagacctg aggctgcgtg
     1561 cgggtcacgt gaaagctgcc atgaccggcg taaggtttgt aggcatcgat ccacatcacg
     1621 tacgccggct gaccggcatg ctgggccgtg ttccatcccg gaatgctgcc gtcggtggca
     1681 gtctgttcga aatccgggtt gatcaagacg ccgcctggca cgcccgacga tgctcccggt
     1741 gtcagcgccc gggcatgcgc ggtctgctgc tgaggagtgc tgcgtccgca agccaccaga
     1801 agcagcacaa tcaatatcga caagcgtttc atggatatcc ttcgacgacg gatgccgtat
     1861 gttagttgca cgccgcccca caagcgaatt ctggcagaat gcgcccatgg aacgcctcac
     1921 cgcccgcgaa ctgttcgaca agatcagcca gcgcctgagc ctgcgctggg tggccggcca
     1981 gcgcggcgaa tcgcgcgcca tcgtgccggc ggaaaaacag ccgcgccggc cttcgctcgc
     2041 cggctatctc aacatcatct atccgaacaa gatccagatc gtcggctccg aggaattgtc
     2101 ctggctcgac agcctcgact cgcgcctgcg ctgggagatg gtcgaaaaga tcatgggctt
     2161 ctcgcccact gcgctgatcg tgaccaagga ccaggccgtg ccggcagatc tgcgcgacgc
     2221 ggccaacgaa tccggtacgc cgatgtgggt cagcagccag cgcggccacg aactgctcac
     2281 ctacctgcaa taccacctcg cgcgcatgct ggcgcggcgc gtgaccctgc acggcgtgct
     2341 gatggaggtg tattccatcg gcgtgctgat caccggcgaa tcgggttcgg gcaagagcga
     2401 actcgcgctc gaactcatca cccgcggcca tcgcctcgtc gccgacgacg caccggaatt
     2461 catccagatc gcaccggacg tgatcgacgg cacctgtccg gaaatgttgc aggacctgct
     2521 cgaagtgcgc ggcctgggta tcctcaacgt gcgcgagatg tttggccaga cggcggtaaa
     2581 gccgtccaaa tacctgcgcc tcatcgtgca cctggacccg ctgccacgcg agcagccgat
     2641 cgaacagggc gggctggagc gcatctacgg cgccaacagc taccgcgaaa tcctcgacac
     2701 gaaaatcccg caggccacca ttcccgtcgc ccccggccgc aacctcgccg tgctggtcga
     2761 ggccgccgtg cgcaaccatg tgctcaagac caagggcatc gatcccgcgc aaaccttcct
     2821 cgaccgccag gcgcaccaga tgcagcggca ggcgccgtgg tgaaaattcg ccgccggaac
     2881 agccgaagga agatcgtcta atgccaagtt ctataaatgc ctcgaaagcc gaaaaacatt
     2941 aatcagcccc gtttttacag aaatagcttg tcgcgataat cctgcacgca tcgatccagc
     3001 gcggcactca gtgtctgctt caacttgtcg ccgttgttgg ccagcaaatc gtctaccttc
     3061 ggcccgtcct tggtttccgg cgagcgatag cggcagatgc ctgacgcgac cacttcgggc
     3121 ttcttgccct tcaacaccgt cgtatcgttg atcttcaccg aggtgttcaa gaccacggtg
     3181 tccttggcga gcg
//
LOCUS       DASRVO010000804         1614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_310512, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000804 DASRVO010000000
VERSION     DASRVO010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310512"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="VFF05_09945"
     CDS             complement(<1..1108)
                     /locus_tag="VFF05_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HZW52175.1"
                     /translation="MKSAIFFCALAFASGTLAATHPFGVHDLVMMNRVGDPQLSPDGK
                     HVAFSMRETDYAANKGSSGIWTLDLSAQGAQPVRLTDKESSASSPRWSADGKSIYYLA
                     QAKGDEYTRVWRMAATPGSAPQRETPWPADINTFKLSPDGTTMLLSIDVFEDCAEHLC
                     TKRPPQAGVEKTTGKIYDRLFVRHWDTWSDGRRSQLYIAPALTPRGGKATVALPRLLS
                     RGIDGDVPSKPFGDDSEFVFSADGKTVYFDVRIAGNSEPWSTNFDVYSVPADGSTAPK
                     NLTAANLAWDASPLPSQDGKTLYYLAMKVPGFEADRFGIMALDLASGKAHEIDPKWDR
                     SAGPLQLSADGKSLYTTTDDNGDHALFAIDIASGK"
     gene            complement(1132..>1614)
                     /locus_tag="VFF05_09950"
     CDS             complement(1132..>1614)
                     /locus_tag="VFF05_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008209672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HZW52176.1"
                     /translation="GLISALAQGVITNNIDWSLIAIGAGIGVAIIMLDEVLGMIKAGP
                     RLPPLAVGLGIYLPTSTTLMVVVGALVGAWFDRRAERGKNGDAVKQLGILLASGLIVG
                     ESLLGVIVAAIVAFSGNAAPLALVGDAFATPSIWIGGIAFVAVTVVLYRWIARMGRSA
                     "
BASE COUNT          287 a          521 c          532 g          274 t
ORIGIN      
        1 ccttgccgct ggcgatgtcg atggcaaaca gcgcgtgatc gccgttgtcg tcggtcgttg
       61 tgtaaagcga cttgccgtcc gcggacagtt gcagcgggcc ggccgaacgg tcccacttcg
      121 gatcgatttc atgtgccttg ccgctggcaa gatccagcgc catgatgccg aagcggtcgg
      181 cctcgaagcc cggcactttc atggcgaggt agtacagcgt cttgccgtcc tgcgacggca
      241 acggcgacgc atcccatgca aggttcgcgg cagtgaggtt cttcggcgcc gtcgagccgt
      301 ccgcgggcac ggaatacacg tcaaagttcg tcgaccaagg ctcgctgttg ccggcgatgc
      361 gcacgtcgaa ataaaccgtc ttgccgtcgg ctgagaacac gaactcgctg tcgtcgccaa
      421 aaggtttcga cggcacgtcg ccatcgatgc cgcgggacaa cagccgcggc agcgccaccg
      481 tggccttgcc gccacgcggc gtcagcgccg gcgcaatgta aagctgcgag cggcggccgt
      541 cggaccaggt atcccagtgg cgcacgaaca ggcggtcgta aatcttgccg gtggtctttt
      601 ccacgcctgc ctgcggcgga cgcttggtgc acagatgctc ggcgcaatcc tcgaacacat
      661 cgatggacag cagcatcgtc gtgccgtcgg gcgagagctt gaacgtgttg atgtcggccg
      721 gccacggcgt ttcgcgttgc ggcgccgaac ccggcgtcgc cgccatgcgc cacacgcgcg
      781 tgtattcgtc gcccttggcc tgggcgaggt agtagatcga tttgccgtcc gccgaccagc
      841 gcggactgga tgcgctggat tccttgtcgg tcaggcgcac gggttgtgca ccctgcgcgg
      901 acaaatccag cgtccagatg ccgctgctgc ccttgttcgc ggcatagtcg gtttcgcgca
      961 tcgagaacgc gacgtgcttg ccgtccggcg agagttgcgg atcgccgacg cggttcatca
     1021 tcacgaggtc gtgcacgccg aacggatgcg tagcggcgag cgtgccggag gcgaacgcga
     1081 gagcgcagaa aaagatagca gatttcattt cgatactcct agcggcaata ctcaggcgga
     1141 ccgacccatg cgcgcgatcc agcggtacag tacgacggtg acggcgacga acgcgatgcc
     1201 gccgatccag atcgacggcg tggcaaaggc gtcaccgacc agggccagcg gcgcggcgtt
     1261 gccggaaaac gcaacgatgg ccgctacgat cacgccgagc aggctctcgc cgacgatcag
     1321 gcccgacgcc agcaggatgc cgagttgctt gaccgcgtcg ccgttcttgc cacgttcggc
     1381 gcgacggtcg aaccaggctc cgaccagggc cccgaccacg accatcagcg tcgtcgaggt
     1441 cggcaggtag atgcccaggc ccacggccag cggcggcagg cgtggaccgg cctttatcat
     1501 gccgaggacc tcatccagca tgatgatcgc cacgccgatg ccggcgccga tggcgatcag
     1561 gctccagtcg atgttattgg tgatcacgcc ctgtgccagc gccgagatca ggcc
//
LOCUS       DASRVO010000805         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_360660, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000805 DASRVO010000000
VERSION     DASRVO010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360660"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1112)
                     /locus_tag="VFF05_09955"
     CDS             complement(108..1112)
                     /locus_tag="VFF05_09955"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZW52177.1"
                     /translation="MNTANSAGFGGAYAREREERGLLVFTGNANRALAAAVCHELNVP
                     LGKALVSRFSDGEVQVEIEENVRGQEVFVIQPTSAPTADNFMELLVIVDALKRASAAS
                     VTAVLPYFGYARQDRRTRSARVPITAKVAAKMIGTVGTDRVLTVDLHADQIQGFFDIP
                     VDNVYSSPVLLADIWRCHGGSDLIVVSPDVGGVARARALAKRLDDADLAIIDKRRPRP
                     NEATVMNIIGDVKDRVCVLIDDIVDTAGTLCAAAAALKKNGARKVVAYCTHPVLSGAA
                     IQNISGSQLDELVVTDTIALADAARACAKIRQLSVAELLGETIRRIAFGESVSSLYVD
                     "
     gene            complement(1226..1300)
                     /locus_tag="VFF05_09960"
     tRNA            complement(1226..1300)
                     /locus_tag="VFF05_09960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1266..1268),aa:Gln,seq:ttg)
BASE COUNT          279 a          520 c          438 g          264 t
ORIGIN      
        1 ctcgcggcgg tgatgatgct cccccgcgac cagggaagcc gtttactgga agactcgcgc
       61 cttccgaagc gggatcggcc cccgatccca aaaaaaatca aaaatgacta gtccacgtac
      121 aaactgctga ccgactcgcc gaacgcgatg cgccggatcg tctcgccgag cagttccgcc
      181 acgctgagct ggcggatttt cgcgcaagcg cgcgccgcat ccgccagcgc gatggtgtcc
      241 gtcaccacca gttcgtcgag ctgagatccg gaaatgttct ggatcgccgc gccggacagc
      301 accggatgcg tgcagtacgc caccaccttg cgcgcgccgt tcttcttcaa cgccgccgcc
      361 gccgcacaca gggtgccggc ggtgtcgacg atatcgtcga tcagcacgca gacgcgatcc
      421 ttcacgtcgc cgatgatgtt catcaccgtg gcctcgttcg gacgcggccg gcgcttgtcg
      481 atgatcgcca ggtccgcgtc gtccaggcgc ttggccaatg cccttgccct ggccacgccg
      541 ccgacgtcgg gactgaccac gatcaggtcg ctgccaccgt ggcagcgcca gatgtcggcc
      601 agcaaaacgg gcgacgagta tacattgtcc accgggatat cgaaaaaccc ctggatctgg
      661 tcggcatgca gatccaccgt caacacgcgg tcggttccga ccgtgccgat cattttcgcc
      721 gccaccttgg ccgttatcgg cacgcgcgcc gaacgcgtgc gccgatcctg ccgggcgtag
      781 ccgaaatacg ggagtaccgc ggtcacgctg gccgccgagg cgcgcttgag cgcatccacg
      841 atgaccagca attccatgaa gttgtccgcg gttggcgccg acgtcggctg gatcacgaac
      901 acttcctggc cgcggacgtt ttcctcgatc tcgacctgca cttcaccgtc ggaaaaccgg
      961 ctgaccagcg ccttgcccag tggcacgttc aactcgtggc aaaccgcagc tgccagcgcg
     1021 cggttggcgt tgccggtgaa gaccagcaag ccgcgctctt cgcgctcgcg cgcgtaggca
     1081 ccgccgaaac ccgctgaatt ggccgtgttc acaccctgct cccgactggc aacgcgtaaa
     1141 tccaattcct ggcgaaagac cgggcatcct gccgccactt tggcgcatcc tgcgccaaca
     1201 tcaaacttgt cgccactccg aaaaatggct gggccggcag gattcgaacc tgcggatgcc
     1261 gggatcaaaa cccggtgcct taccgcttgg cgacggccca aaggtattgc agcgaaagat
     1321 tgggcatcca tgccgcaact ttcgcggaaa cggtcgcagc aaagctgcgc cacgttttca
     1381 cgacacagcg ctatcgcgct gtgcgcggca cctccctgtg ccgaattgtc ctgcgcttgt
     1441 ccgcgagatc ggcatcctgc cgcaactttc gcgtcgcgag cccgaagcaa gttcgggctg
     1501 c
//
LOCUS       DASRVO010000806         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_76097, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000806 DASRVO010000000
VERSION     DASRVO010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76097"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VFF05_09965"
     CDS             complement(<1..836)
                     /locus_tag="VFF05_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZW52178.1"
                     /translation="MPTYRTEHDSMGELKVPADALWGAQTQRAVDNFPISGLALPREF
                     IRALGLIKAAAADANLDLKHLKPTQAAAIRKAAQRVAAGEFDKHFPIDIFQTGSGTSS
                     NMNANEVIAHIASQGGKAIHPNDHVNYGQSSNDVIPTAIHVSASLAAHEQLLPALKHL
                     QKTIDRRARELKNVAKTGRTHLMDAMPVTFGQELSGWSAQVASGIARIEDAGRRMRRL
                     PQGGTAVGTGINADPKFAKAFCTELSKAAKVKFTSMDNFFEGMASMDAAVELSGQLKT
                     LA"
BASE COUNT          158 a          322 c          325 g          213 t
ORIGIN      
        1 catgccagcg ttttcaactg gccggacagt tccaccgcgg cgtccatgga tgccatgccc
       61 tcgaagaaat tgtccatcga ggtgaatttc actttcgcgg ccttcgacaa ttcggtgcag
      121 aacgccttgg cgaatttggg atcggcgttg ataccggtgc ctaccgccgt tccgccctgc
      181 ggcaggcggc gcatgcgcct gccggcgtcc tcgatgcgcg cgatgcccga cgcgacctgc
      241 gccgaccagc ccgagagttc ctgcccgaac gtcaccggca tcgcatccat cagatgcgtg
      301 cgaccggtct tggcgacgtt cttcagttcg cgtgcgcgcc tgtcgatggt tttctggagg
      361 tgcttcaacg ccggcaacaa ttgctcgtgc gcggccagcg acgcactgac gtggatcgcg
      421 gtcgggatca catcgttgga gctttgtcca tagttgacgt ggtcattggg atggatcgcc
      481 ttgccgccct gcgaggcgat gtgtgcaatc acctcgttgg cattcatgtt ggacgaggtg
      541 cccgaacccg tctggaagat gtcgatcggg aagtgcttgt cgaactcgcc tgcggcgacg
      601 cgctgcgcgg ccttgcggat cgccgcagct tgcgtgggct tgaggtgctt gagatcgagg
      661 ttcgcatcgg cggcggcggc cttgatcaga ccgagcgcgc gaatgaattc gcgcggcagc
      721 gccaggcccg agatcgggaa attgtccacc gcgcgctggg tctgcgcgcc ccacagtgcg
      781 tctgcgggta ccttgagttc gcccatgctg tcgtgctcgg tgcggtaggt cggcattgca
      841 atcgatccgt gcgggaagct cctcattata acgagcggcc gccgttacaa tgcccgcttc
      901 gcgacgccac ggacccgccg ccatgcaact cgatccgctg accgcccttt cgccgctgga
      961 tggacgctat gcatccaggg tcgatgcctt gcgcccgatc ttcagcgaat acggcctg
//
LOCUS       DASRVO010000807         1606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_24170, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000807 DASRVO010000000
VERSION     DASRVO010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24170"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFF05_09970"
     CDS             <1..1115
                     /locus_tag="VFF05_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52179.1"
                     /translation="HLKPDAAGGFELDHDKLRTTVTTAMRMLDNVIDLNYYAVKKARD
                     ANMRHRPVGLGVMGFQDTLYQLRVPYASQEAVAFADRSMEAICYHAYWASTELARERG
                     RYSSYKGSLWDKGILPLDTLSLLERQRGGWVEVDRSATLDWDALRRKIARDGMRNSNC
                     IAIAPTATISNIVGVDASIEPCFGNLSVKSNLSGEFTVINHYLVRDLKRLGLWDEVMV
                     MDLKHFDGSLRPIDRVPQEMKALYATAFEVEPRWLVEAGARRQKWIDQAQSLNIYMAG
                     ASGKKLHETYTLAWLRGLKTTYYVRTTSATQAEKSTVQSRRLNSASSGGVSALDAAAA
                     AAHAQVDAAAQPHANATPATDIGFCAIDDPTCEACQ"
BASE COUNT          307 a          520 c          516 g          263 t
ORIGIN      
        1 accacctcaa gcccgatgcg gccggcggct tcgagctcga tcacgacaag ctgcggacga
       61 cggtcaccac cgcgatgcgg atgctggaca acgtgatcga cctcaactac tacgccgtga
      121 agaaggcgcg cgacgccaac atgcggcacc ggccggtcgg cctcggcgtg atgggattcc
      181 aggacacgct ctaccaactg cgggttccgt atgcctcgca ggaagcggtg gcattcgccg
      241 accggtcgat ggaagcgatc tgctatcacg cctactgggc gtcgaccgaa ctggcgcgcg
      301 agcgcggccg ctactccagc tacaagggtt cgctgtggga caagggaatc ctgccgctgg
      361 acacgctgag cctgctggag cgccagcgcg gcggctgggt cgaagttgac cgctccgcca
      421 cgctggactg ggacgcgctg cgccggaaga tcgcgcgcga cggcatgcgc aattccaact
      481 gcatcgccat cgcgccgacg gcgacgattt ccaacatcgt cggcgtggat gcctcgatcg
      541 aaccctgctt cggcaacctg tcggtgaaat cgaacctgtc cggcgagttc acggtgatca
      601 accattacct ggtgcgagat ctgaagcggc tggggctgtg ggacgaggtc atggtgatgg
      661 acctcaagca tttcgacggc tcgctgcgcc cgatcgaccg tgtgccgcag gagatgaagg
      721 cgctttatgc gaccgcgttc gaagtggaac cgcggtggct ggtggaagcc ggcgcgcgcc
      781 ggcagaagtg gatcgaccag gcgcagtcgc tcaacatcta catggccggc gcctcgggca
      841 agaagctgca cgagacctac acgctggcct ggctgcgcgg cctgaagacc acctattacg
      901 tgcgcacgac gagcgcgacg caggccgaga aatcgaccgt gcagtcgcga cgcctgaact
      961 cggcctcgag cggcggcgtg agcgcgctcg atgccgcggc cgccgcggcg cacgcgcagg
     1021 tcgatgccgc ggcgcagccg catgcgaacg cgacgccggc gacggacatc gggttctgcg
     1081 cgatcgacga tccgacgtgc gaggcatgtc agtgaacacg agtcgatcgg tcgtcgcgcg
     1141 atgcggtgat cgatcgatgt gtcggcgttc tccgacagaa cgtcgatgca attgagcaac
     1201 acaatcgcgc ggtgacgcgg ctgcgcgctt tgcatttcgc aatcactgct tccataatcc
     1261 gcacattgga aaacttatgt tgacctggga cgaagaagtc aagcccattt cgccagcatc
     1321 gaataacggc tcgctcccga gccgggtgat cccgtcaccc attccgcaag tttccgctct
     1381 ccctcatctc gctgccggcc tcgcgacgca ctcaccgatg gtcgtgcgcg ccgcgaagcc
     1441 ggcgagcgcg cgccgcgtga acgccgccga caagcgcatc atcaacggcc aggccgaagg
     1501 cgtgctgggg cttaatgctt ccatgttcaa gcgctatctg cattacattg cgaatcgtcg
     1561 cgccacgcag atcggcctcg aagcgctctt cccgaacgag gaaaac
//
LOCUS       DASRVO010000808         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_178551, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000808 DASRVO010000000
VERSION     DASRVO010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178551"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="VFF05_09975"
     CDS             <1..1613
                     /locus_tag="VFF05_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZW52180.1"
                     /translation="HTRRGEVLRTVPRDNEAINECWLSDRDRYSHQGLSASDRATKPM
                     IRKDGELVEVTWDEAIAFVADGLKKSGGDLGALVAPLASSEEGFLLAQILRALGSDAV
                     DHRLRTLDFTDGAPVAAFEMKVADIEKARAIALIGCNPRHEMPLLAHRIRKAWKAGAK
                     VFAINPLDFDLNFDLAAKTIVTPAAMVDALLKLAKAVNDAGHLPESHDLAQLISGIQA
                     DDASRALFKALNDASAKVLILGECAAQHPQASVLRAAARFVAKHTGCAYDEVPSGAND
                     VGLARAGAQPQGQGRNAAAMLAQPPKHLIVYQAGSQDTSSSAAFDAARSNASFCVYIG
                     SYACNGVKRTAHAVLPIGLPPESDGSYVNVDGSVQTLAAGAKAPGDARPGWKVLRALG
                     AALGVAGFDFTEFAQLRDLVAPKLRMGSAKPHAGKLAQRPTEASGLVRIATVPMYRAD
                     QVLRRCAALQAHPLTGKAAIALNPEDALALGLANDTSAKVSGNSAEAVLPVVVTRMVP
                     KGAAWIESTWPETRALPSTGAVLTVARA"
     gene            1610..2695
                     /gene="nuoH"
                     /locus_tag="VFF05_09980"
     CDS             1610..2695
                     /gene="nuoH"
                     /locus_tag="VFF05_09980"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010375869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZW52181.1"
                     /translation="MSVFDPVRNFFLSYGDPGLVAWLVVLILCIAVPVILAVAFYVWW
                     ERKVLGWMHVRMGPNSVGPAGLAQTFADVFKLLFKEYLLPAKASKLLFVLAPLVALTP
                     ALAAWAVIPFDQKAVLADVNAGLLYLLAMTSMRVYGIILAGWSSNSKYAFLGAMRSAA
                     QVVSYEIAMGMCLVGVLICAGSLNMSDIVRAQAGAHGLFSWFWLPLLPLFVIYFVSGV
                     AETNRAPFDVAEGESEIVAGFHVEYSGAPFAVFFLTEYANMILVSFLASVLFLGGWLS
                     PFQGWITADVSPWVDWIWKGGWEWLFAKVFLFAFMYLWFRAAFPRYRYDQIMRLGWKV
                     FIPIALVWIGVAGCLVYFGVVGQGVSP"
     gene            2692..>2857
                     /locus_tag="VFF05_09985"
     CDS             2692..>2857
                     /locus_tag="VFF05_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006381128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HZW52182.1"
                     /translation="MSRVVRYFKSLLLLELFKGLALTGKYMVKPKYTMRYPMEKIPQS
                     NRFRGLHALRR"
BASE COUNT          445 a          900 c          947 g          565 t
ORIGIN      
        1 tgcacacgcg ccgcggcgaa gtgctgcgca cggtgccgcg cgacaacgaa gcgatcaacg
       61 aatgctggct gtccgaccgc gaccgctatt cgcaccaggg cctgtccgct agcgaccgcg
      121 cgaccaagcc catgattcgc aaggacggcg aactggtcga agtgacgtgg gacgaggcca
      181 tcgccttcgt cgccgacgga ctgaagaaat ctggcggcga tctcggcgcg ctggtcgcgc
      241 cgctggcgtc gagcgaagaa ggcttcctgc tcgcgcagat cctgcgcgcg cttggcagcg
      301 atgccgtcga tcatcgcctg cgcacgctgg atttcaccga cggcgcgccg gtcgcggcgt
      361 tcgagatgaa ggtggccgac atcgaaaagg cgcgtgcgat cgcgctgatc ggctgcaatc
      421 ctcgccacga gatgcccttg cttgcgcacc gcattcgcaa ggcgtggaag gccggcgcga
      481 aggttttcgc gatcaatccg ctggatttcg atctgaattt cgatctggcc gcgaaaacca
      541 tcgtcacgcc ggccgcgatg gtcgatgcgc tgctcaagct cgccaaggcg gtgaacgatg
      601 ccgggcatct gccggaatcg cacgatctgg cgcagctcat ttccggaatt caggctgacg
      661 atgcttcgcg cgcactgttc aaggcgttga acgatgcatc ggccaaagtg ctgatcctcg
      721 gcgaatgcgc cgcacagcat ccgcaggcat ccgtcttgcg tgctgcggca cgcttcgttg
      781 ccaagcacac ggggtgtgcc tatgacgagg ttccatcggg cgcgaacgat gtcggtctcg
      841 cgcgcgcggg cgcgcagccg caagggcagg gcaggaacgc cgccgcgatg ctggcacagc
      901 cgcccaagca cctgatcgtg tatcaggccg gttcgcagga tacgtcctcg tccgccgcgt
      961 ttgacgccgc gcgcagcaat gcgtccttct gcgtctacat cggttcctac gcctgcaatg
     1021 gcgtgaagcg cacggcgcat gcggtgctgc cgatcggcct gccgccggaa agcgacggct
     1081 cttacgtcaa tgtcgacggc agcgtgcaga cgctcgcggc cggtgcgaag gcgccgggcg
     1141 atgcgcgccc gggctggaaa gtcctgcgcg cgctcggtgc ggcgctgggt gtggccggtt
     1201 tcgatttcac cgaattcgcg cagctgcgcg atctggttgc gccgaaactt cgcatgggat
     1261 ccgcaaagcc gcatgccgga aaattggcgc aacgtccgac ggaagcgtcg ggtttggtgc
     1321 gcatcgccac ggtgcccatg tatcgcgccg atcaggtgct gcggcgttgt gcggcgctgc
     1381 aggcgcatcc gctgaccggc aaggccgcga tcgcgctcaa tcccgaagac gcgctggcgc
     1441 tgggactcgc gaatgacact tccgccaagg tttccggcaa cagtgccgaa gccgtgctgc
     1501 cggtagtggt gacgcgcatg gtgcccaagg gcgcggcgtg gatcgaatcc acctggccgg
     1561 aaacgcgcgc gctgccgtcg accggcgcag tactgacggt tgcgagggcc tgagcgtgtt
     1621 cgatcccgta cgcaatttct tcctgagtta cggcgatccc ggcctggtcg cctggctggt
     1681 ggtcctgatc ctgtgcatcg ccgtgccggt gattctcgcc gtcgcgttct atgtctggtg
     1741 ggagcgcaag gtgctcggct ggatgcacgt gcgcatgggg ccgaacagcg tgggtccggc
     1801 cggattggcg cagaccttcg ccgacgtctt caagctgctg ttcaaggaat acctgctgcc
     1861 ggccaaggcg agcaagctgc tgttcgtgct cgcgccgttg gtcgcgttga cgcccgcgtt
     1921 ggcggcttgg gcggtgattc cgttcgacca gaaggccgtg ctcgccgacg tcaacgccgg
     1981 cctgttgtac ttgctcgcga tgacctcgat gcgcgtgtac ggcatcatcc tcgccggctg
     2041 gtcgtccaat tccaaatatg ccttccttgg cgcgatgcgc tccgccgccc aagtcgtcag
     2101 ctacgagatc gcgatgggca tgtgcctcgt cggcgtgctg atctgcgccg gcagcctgaa
     2161 catgtccgac atcgtccgcg cgcaggccgg cgcgcacgga ctgttttcgt ggttctggct
     2221 gccgctgttg ccgctgttcg tgatctattt cgtgtcgggc gttgccgaga ccaaccgcgc
     2281 cccgttcgac gtggccgaag gcgaatcgga aatcgtggcc ggtttccacg tcgagtattc
     2341 cggtgcgccg ttcgcggtgt ttttcctaac cgaatacgcg aacatgatcc tggtttcctt
     2401 cctcgcctct gtgttgttcc ttggcggctg gctgtcgccg ttccagggat ggatcacggc
     2461 ggacgtatcg ccatgggtgg actggatctg gaaaggcggc tgggaatggc tgttcgccaa
     2521 ggtcttcctg ttcgcgttca tgtacctgtg gttccgcgcc gcgtttccgc gctaccgcta
     2581 cgaccagatc atgcgcctgg gctggaaagt gttcataccg atcgcgctgg tctggatcgg
     2641 cgtggccggt tgtctcgtct atttcggtgt ggtcggacag ggagtctcgc catgagccgc
     2701 gtcgtgcgct acttcaagag cctgctgctg ctcgaactgt tcaagggcct ggccctgacc
     2761 ggcaagtaca tggtgaaacc gaaatacacc atgcgctacc cgatggagaa gatcccgcag
     2821 tcaaaccgct tccgcggcct gcacgcgctg cgccgct
//
LOCUS       DASRVO010000809         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_336340, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000809 DASRVO010000000
VERSION     DASRVO010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336340"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFF05_09990"
     CDS             <1..576
                     /locus_tag="VFF05_09990"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972141.1"
                     /note="Acs; catalyzes the conversion of acetate and CoA to
                     acetyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HZW52183.1"
                     /translation="GECEGNLVLLDSWPGQMRTVYGDHQRFIDTYFKAYPHMYFIGDG
                     ARRDADGYYWITGRVDDVINVSGHRLGTAEVESALVAHTTVAEAAVVGFPHDIKGQGI
                     YAYVTLKTGVDSSEELRKELVAHVRHEIGPIATPDHLHWAPALPKTRSGKIMRRILRK
                     IAENAPDQLGDISTLADPSVVQHLVDTRLVK"
     gene            573..>1092
                     /locus_tag="VFF05_09995"
     CDS             573..>1092
                     /locus_tag="VFF05_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZW52184.1"
                     /translation="MTTASTIARFTPALPPQPLAHEEIHLWFFPQWEKTSDAARSAPV
                     RGLLAAYLDRPVDTVRIERGEHGKPRLTGGELEFNLAHTGAALLLGISHDVVLGVDLE
                     NAQRRTRSIELARRFFTPAETAALESLPDELRQATFLRLWCAKEAALKAQGRGIGFGL
                     DRVEFAIDATGAV"
BASE COUNT          197 a          360 c          348 g          187 t
ORIGIN      
        1 ggcgaatgcg aaggcaatct ggtcctgctc gattcctggc cgggccagat gcgcacggtg
       61 tacggcgatc accagcgttt catcgacacc tatttcaagg cgtatccgca catgtacttc
      121 atcggcgacg gcgcgcgtcg cgacgccgac ggctattact ggatcaccgg ccgcgtcgac
      181 gatgtgatca atgtgtccgg gcaccgtctt ggcaccgcgg aagtggaaag cgcgctggtc
      241 gcgcacacga cggtggccga agcggcggtc gtcgggtttc cgcacgacat caagggccag
      301 ggcatctacg cctacgtcac gctcaagacc ggcgtggatt cgagcgaaga actgcgcaag
      361 gagctggtcg cgcacgtacg ccacgaaatc ggcccgatcg ccacgcccga tcatctgcat
      421 tgggcgcccg cgctgccgaa gacgcgctcg ggcaagatca tgcgtcgcat cctgcgcaag
      481 atcgccgaga acgcgccgga ccagctcggc gacatttcca cccttgccga tccgtccgta
      541 gtccagcacc tggtggatac gcggctggtg aagtgacgac cgcgtcgacg atcgcgcgtt
      601 tcactccggc cttgccaccg caaccgctgg cgcacgagga aatccacttg tggttttttc
      661 cacaatggga aaagacgtcc gatgccgccc ggtcggcgcc cgtgcgcggc ctgctcgcgg
      721 cgtatctgga tcgccccgtc gacaccgtcc gcatcgaacg cggcgaacac ggcaagccgc
      781 gcctgaccgg cggggagctc gaattcaatc ttgcgcacac gggggctgcg ctgttgcttg
      841 gcatcagcca tgacgtcgtt cttggcgtgg atctggagaa cgcacaacgc aggacccgat
      901 cgatcgaatt ggcgcgacga tttttcacac cggccgagac ggcggcgctc gaaagcctgc
      961 ccgatgagct gcgccaagcc acctttctgc gcctgtggtg cgccaaggaa gccgcactca
     1021 aggcgcaggg ccgcggcatc ggcttcggcc tggatcgcgt cgagttcgcc atcgatgcca
     1081 ccggtgcggt tg
//
LOCUS       DASRVO010000810         1409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_360823, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000810 DASRVO010000000
VERSION     DASRVO010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360823"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFF05_10000"
     CDS             <1..554
                     /locus_tag="VFF05_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HZW52185.1"
                     /translation="DIEPAEALRVLREAQILCALRDGPFGAEGVNARVTRRLAARFGF
                     DAGEAWYHGRPVIVTRNDYARGLFNGDVGIALDRGDGLRVWFDAGDASGPRSFSPRAL
                     PAHETAWAITIHRSQGSEYDRVAVLLPPDAQHRILSRELVYTAVSRARAGATLWASAD
                     SLRAAVTQPVMRRSGLGERLIVA"
     gene            618..1274
                     /locus_tag="VFF05_10005"
     CDS             618..1274
                     /locus_tag="VFF05_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZW52186.1"
                     /translation="MLEILIAGGWAMVPILLCSAVALAITLERFWSLRRKTVVPPELG
                     AQVLAWARTRKLDPGHIETLRVNSPLGEILAAALSVRNRSREIIKERVEDTGRHVVHR
                     LERFLNTLGTIALISPLLGLLGTVFGLIEMFFAVMVSGVGDPLKMAGGIGQALVCTAS
                     GLCVAIPAYFFHRYFRGRVADYVVDMEEQTIQLIDELSAHAEPAAPAAPAARAPRAAH
                     "
     gene            1276..>1409
                     /locus_tag="VFF05_10010"
     CDS             1276..>1409
                     /locus_tag="VFF05_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZW52187.1"
                     /translation="MRLGAQRQDDFEINVISLIDVMLTLLMFFVMTTTFVQHSALKVT
                     L"
BASE COUNT          203 a          475 c          476 g          255 t
ORIGIN      
        1 cggacatcga gcccgccgaa gcgctgcgcg tcctgcgcga ggcgcaaatc ctgtgcgcac
       61 tgcgcgacgg gccgttcggc gcggaaggcg tcaacgcacg ggtgacgcga cggctcgccg
      121 cgcgtttcgg cttcgatgcc ggggaagcct ggtaccacgg ccggcccgtc atcgtcaccc
      181 gcaacgatta cgcgcgcggc ctgttcaacg gcgacgtcgg catcgcgctg gatcgcggcg
      241 acggcctgcg cgtgtggttc gatgcgggcg atgcaagcgg tccgcgcagt ttctcgccgc
      301 gcgcgctgcc tgcgcacgaa accgcctggg ccatcaccat ccatcgcagc cagggctcgg
      361 aatacgatcg cgtcgccgta ttgctgccac ccgacgcgca acaccgcatc ctcagccgcg
      421 aactcgtcta caccgccgtc tcgcgcgccc gcgcgggcgc gacgctgtgg gccagtgccg
      481 attcactacg cgctgccgtg acgcagccgg tgatgcggcg gagtgggttg ggggagaggt
      541 taatcgtggc ttgattcgca gcgcaaatcc gcccgtcgct gttatgattg ctgcggttcg
      601 ttgacggagt ctcgcgcgtg cttgaaatcc tgattgccgg cggctgggcg atggtgccga
      661 tcctgctgtg ttcggcggtg gcgctggcca ttaccctgga acgtttctgg tcgttgcggc
      721 gcaagaccgt ggtgccgccg gaactcggcg cgcaggtgct ggcctgggcg cgcacgcgca
      781 agctcgatcc cggccacatc gaaaccctgc gcgtgaattc gccgctcggc gaaatcctcg
      841 ccgcggcgct gtcggtgcgc aaccgttcgc gcgagatcat caaggagcgc gtcgaggaca
      901 ccggccgcca cgtcgtgcac cggctcgaac gcttcctcaa cacgctcggc accatcgccc
      961 tgatctcgcc gctgctcggc ctgctcggca ccgtgttcgg cctgatcgag atgttcttcg
     1021 cggtgatggt gtccggcgtc ggcgatccgc tgaagatggc cggcggcatc ggccaggcgc
     1081 tggtgtgcac cgcctcgggt ctgtgcgtgg cgatcccggc gtatttcttc caccgctatt
     1141 tccgcgggcg cgtcgccgac tatgtggtcg acatggaaga gcagaccatc cagttgatcg
     1201 acgaactgtc cgcgcacgcg gagccggcag ctccggcggc accggcagcg cgcgcgccgc
     1261 gcgcggcgca ctgacatgcg tcttggtgcg caacggcagg acgatttcga gatcaacgtg
     1321 atctcgctga tcgacgtcat gctcacgctg ctaatgttct tcgtcatgac gacgacgttc
     1381 gtgcagcact cggcgttgaa ggtgaccct
//
LOCUS       DASRVO010000811         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_153073, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000811 DASRVO010000000
VERSION     DASRVO010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153073"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..435)
                     /locus_tag="VFF05_10015"
     CDS             complement(100..435)
                     /locus_tag="VFF05_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52188.1"
                     /translation="MSIIVGFIAAAFSISVSAKDTSCSIAPPANASNAQLAALAKVTK
                     GAAQTSALKAIGVAGKMAPQSAELKAENGCLIWSFDIRNAGTGATYAVIVDAGNGAAI
                     SVNGQSPAK"
     gene            complement(455..1483)
                     /locus_tag="VFF05_10020"
     CDS             complement(455..1483)
                     /locus_tag="VFF05_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007804557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HZW52189.1"
                     /translation="MRRSSLLAAIALAFPFIAAAAAPHYAVVQRYAIGQPGGWDYLTY
                     SADGHRLYVSRFDRVLVIDADTGKIEATITGTDGVHGVALVPKLGRGYTSNGRADTLT
                     EFDLATSRVLRTIAVEDHNPDALIYDPASAHLFVFDGRSNEASVVDPVAGKLVSKIAL
                     SGKPEFAASDGAGNVYVNIEDTAQLARIDSATNKVTAVWKLADCEEPSGLALDIVHHR
                     LFSVCQNKRMAVTDAQSGKPVASVAIGEGPDGAGFDAERGLIYSTNGHDGTLTIVHED
                     DPDHYRVIANVPTQKSARTLALDADGNRIFTVAAEFGPRPAPTKENPHPRPTIVDGSF
                     AVLVIGNH"
     gene            complement(1572..>2380)
                     /locus_tag="VFF05_10025"
     CDS             complement(1572..>2380)
                     /locus_tag="VFF05_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZW52190.1"
                     /translation="SAQAFGYNGQFQYDLAAVVEFIHTATLLHDDVVDESELRRGRET
                     ANALFGNAASVLVGDFLYSRAFQMMVEADDMRVMRILADTTNRIAEGEVLQLLNIHNP
                     DVDEAAYLRVIERKTAVLFAAATRLGGLLGALPAAQENALAEYGLNLGFAFQIADDVL
                     DYVADAGTLGKNIGDDLAEGKVTLPLIYTLERADSAQAAHIRRAIETGGLDALDAILA
                     AIRGSGAVARARTRAQGYADAAKAALSTLPQTVARAALEALADYALQRDH"
BASE COUNT          379 a          719 c          821 g          461 t
ORIGIN      
        1 agacatgccg ctgcggtcga ccgcgcggcg cgtcgattgt cggcaggcag ttcagcgctg
       61 ggcattgtcc tggtgtgcgt cgccgtgcgt ttcgtcggct tacttcgctg gggattggcc
      121 attgacggag attgccgctc cgttgcccgc atcgacgatg accgcatagg tcgcgccggt
      181 tccggcattg cggatgtcaa acgaccagat caggcagccg ttttcggcct tgagttcggc
      241 gctctggggc gccattttgc ccgctacgcc gatcgccttg agcgcgcttg tctgtgcggc
      301 gcctttcgtc accttggcga gggcggcaag ttgtgcattg gaggcgttcg ccggcggcgc
      361 gatggaacat gacgtgtctt tggcgctgac ggaaatcgag aacgcggctg cgataaaacc
      421 gacgatgatg gacattttca tgaatgaatt ctcctcagtg attgccgatg acgagaacgg
      481 cgaagctgcc atcgacgatg gtcggccgcg ggtgcggatt ttcctttgtc ggcgccggac
      541 gcgggccaaa ttcggcggcg acggtgaaaa tgcggttgcc gtcggcatcg agcgccagcg
      601 tgcgcgcgct cttctgcgtc ggcacgttcg cgatcacgcg gtaatggtcg ggatcgtctt
      661 cgtgcacgat ggtgagcgtg ccgtcgtggc cattcgtgct gtagatgagc ccgcgttcgg
      721 cgtcgaatcc ggcgccatcg gggccttcgc cgatcgcgac gctggccaca ggtttgcccg
      781 attgcgcgtc ggtcaccgcc atgcgcttgt tctggcagac cgaaaacagg cgatggtgca
      841 cgatgtcgag ggcgagcccc gaaggttcct cgcaatcagc gagtttccac acggcggtga
      901 ctttgttcgt ggccgaatcg atgcgcgcca gttgcgcggt gtcttcgata ttgacgtaga
      961 cattgccggc gccatcgctg gcggcaaact ccggtttccc ggacagtgca atctttgaca
     1021 ccagcttgcc cgcgaccgga tcgacgacgc tggcttcgtt gctgcggccg tcgaagacga
     1081 acagatgtgc ggaagccgga tcgtagatca gcgcatcggg attgtggtct tcgaccgcaa
     1141 tcgtacgcag caccctggac gtggcgagat cgaattcggt gagcgtgtcg gcgcggccgt
     1201 tgctggtata gccgcggccg agcttgggca cgagggcgac gccgtgcacg ccgtcggtgc
     1261 cggtaatcgt cgcctcgatt ttgccggtgt cggcatcgat caccagcacg cggtcgaagc
     1321 ggctgacata caggcgatgc ccgtcggcgg aataggtgag gtagtcccag ccgccgggct
     1381 ggccgatggc atagcgctgc acgacggcgt aatgcggtgc cgcggcagcg gcgatgaagg
     1441 gaaaggcgag ggcaatggcg gccagcaggc tggatcggcg catcggtggc tcctggatgg
     1501 tgaggggcgc cggcagtcta gtccgtcggc gtgaatgttt tccaaagcac gatttccgaa
     1561 gcgccgcgcg gtcagtggtc gcgctgcaac gcgtaatccg ccagtgcttc cagcgcagcc
     1621 cgcgccacgg tttgcggcaa tgtggacaat gccgccttgg ccgcgtcggc gtagccttgc
     1681 gcgcgcgtgc gtgcccgcgc aaccgcgccg ctgccgcgga tcgcggcgag gatggcatcg
     1741 agcgcatcca gtccgccagt ttcgatggcg cggcggatgt gcgccgcctg cgcggaatcg
     1801 gcgcgttcga gcgtgtagat cagcggcagc gtgaccttgc cttcggcgag gtcgtcgccg
     1861 atgttcttgc cgagcgtgcc ggcatcggcg acgtagtcga gcacgtcgtc ggcgatctgg
     1921 aaggcgaagc cgagattcag cccgtactcc gccagagcgt tttcctgtgc cgccggcagc
     1981 gcgccgagca gtccacccag gcgcgtggcg gcggcgaaca acaccgcggt cttgcgctcg
     2041 atcacgcgca aatacgctgc ttcgtccacg tccggattgt ggatgttgag cagttgcagc
     2101 acctcgccct cggcgatgcg gttggtggtg tcggcgagga tgcgcatcac gcgcatgtcg
     2161 tccgcctcga ccatcatctg gaacgcgcgc gaatacagga aatcgccgac cagtacgctg
     2221 gcagcgttgc cgaacagcgc attagccgtt tcgcgtccgc ggcgcaactc ggattcgtcc
     2281 accacgtcgt cgtgcagcag cgtcgcggtg tggatgaatt ccaccaccgc cgccaggtcg
     2341 tactggaact ggccgttata accgaaggcc tgtgccgaca
//
LOCUS       DASRVO010000812         1351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_153084, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000812 DASRVO010000000
VERSION     DASRVO010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153084"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFF05_10030"
     CDS             complement(<1..306)
                     /locus_tag="VFF05_10030"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HZW52191.1"
                     /translation="MADRVYFFDDINSIAFSIGPLAVHWYGLMYLGGFLGGWWLGNHR
                     LKQGRLPVSEQAFSDLAFYAMIGVIAGGRVWYMLSYISPDWIWHDPLALFRVWDGGMS
                     "
     gene            394..>1351
                     /locus_tag="VFF05_10035"
     CDS             394..>1351
                     /locus_tag="VFF05_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HZW52192.1"
                     /translation="MNRLATESSPYLRQHADNPVDWWPWCDEALAAARALDKPLLLSI
                     GYSACHWCHVMAHESFEDAATAALMNELFICIKVDREERPDLDRIYQLAHQALTRRGG
                     GWPLTVFLTPDDQLPFYAGTYFPREPRYAMPPFAQVLREVRRWWDERRGEVREQNTAL
                     AAFLADHGRGESTADTLDEAPIRAALEQITASFDPEFGGHRGAPKFPHCTEMELLRDL
                     RKPEQVEFTLARMADGGIHDQIGGGLARYSVDERWEIPHFEKMLYDNAQLLPLFATHS
                     PEVAHGIFAWLVREMTAESGGLFAALDADSEHEEGKFYGWSRD"
BASE COUNT          271 a          445 c          418 g          217 t
ORIGIN      
        1 ggacatgccg ccgtcccaga cccggaacag cgcgagcgga tcgtgccaga tccagtccgg
       61 agaaatgtac gacagcatgt accaaacgcg cccgccggcg atcacaccga tcatcgcgta
      121 gaacgccaag tcggagaacg cctgctcgct caccggcagg cgcccttgct tcagtcgatg
      181 gttgcccagc caccagccgc cgagaaaacc gccgaggtac atgaggccat accagtgcac
      241 ggccagcggg ccgatggaaa acgcgatgga attgatgtcg tcgaagaagt acacgcgatc
      301 cgccataaac cattgaagac ggaagtttag cggatcggct ggcgtctgcg cctgcatttc
      361 ctttgccttc cgatttgggg ttaccctgac cgcatgaatc gactagcgac cgaatccagc
      421 ccctacctgc gccagcacgc ggacaatccc gtggactggt ggccatggtg cgacgaggcg
      481 ctcgccgccg cgcgcgcgct ggacaagccc ctcctgcttt ccatcggcta cagcgcctgc
      541 cattggtgcc atgtgatggc gcacgagagc ttcgaggacg cagccacagc ggcgctgatg
      601 aacgagctgt tcatttgtat aaaagtggat cgcgaagaac gccccgacct cgaccgcatc
      661 taccagctcg cgcaccaggc cctgacccgg cgcggcggcg gctggccgct gaccgtgttc
      721 ctgaccccgg acgaccaact gccgttctac gccggcacgt attttccacg cgagccgcgt
      781 tacgccatgc cgccgttcgc gcaggtgctg cgcgaggtgc ggcgctggtg ggacgaacgc
      841 cgcggcgagg tgcgcgagca aaacaccgcg ctggcggcat ttctcgccga tcacggccgc
      901 ggcgaatcca cggcggatac gctggacgag gcaccgatcc gcgccgcgtt ggaacagata
      961 accgcaagct tcgatcccga attcggcggt catcgtggcg cgccgaagtt tccgcattgc
     1021 acggaaatgg aactcctgcg agaccttcgc aaacccgaac aggtcgaatt cacgctcgcg
     1081 cgcatggccg acggcggcat ccacgaccag atcggcggcg gcctagcgcg ctacagcgtg
     1141 gacgagcgct gggaaatccc gcacttcgag aagatgctgt acgacaacgc gcaactgctg
     1201 ccgttgtttg cgacgcacag tcccgaagtt gcgcacggca tcttcgcctg gctcgtgcgc
     1261 gagatgaccg cggaatccgg aggacttttc gcggcgctgg atgccgactc cgaacatgag
     1321 gaaggcaaat tctacggctg gtcgcgcgat g
//
LOCUS       DASRVO010000813         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_76244, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000813 DASRVO010000000
VERSION     DASRVO010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76244"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..825)
                     /locus_tag="VFF05_10040"
     CDS             complement(7..825)
                     /locus_tag="VFF05_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52193.1"
                     /translation="MSTILVFGASGAIGRFVLPLFAPDHRVLPVSRQARPGWICADLN
                     DPAAIWPDAEIVLSLGPLDAFADWLERSPAAALRRIVAMSSMSAQTKQDSPDPAERAL
                     AQRLRGAEARVREIADRRAIAWTIFRPTLIYGAGIDKTLAPLARFARRWRVLPVPFGA
                     SGLRQPVHAADLAAACHAVISQPVTFGKGYELGGGERLRFDAMLRRVREAVPGFVLPL
                     PIPLTGLHLLARPARIAPGALARLRIDLIAVNADAIREFDYSPRPFRAGEILPQ"
     gene            942..1370
                     /gene="rplM"
                     /locus_tag="VFF05_10045"
     CDS             942..1370
                     /gene="rplM"
                     /locus_tag="VFF05_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZW52194.1"
                     /translation="MKTISAKAETVKRDWYLVDASNKTLGRLASELARRLRGKHKTVF
                     TPHVDTGDYLVVINAEKITATGNKMDDKIYHHVTGYIGNIKSIALKDLLVKHPERAIE
                     IAVQGMLPKNPLGRAMYRKLKVYKGPNHPHTAQQPQVLDI"
     gene            1373..1765
                     /gene="rpsI"
                     /locus_tag="VFF05_10050"
     CDS             1373..1765
                     /gene="rpsI"
                     /locus_tag="VFF05_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZW52195.1"
                     /translation="MATTQNYGTGRRKSSAARVFLRPGKGSIVVNGKALDEFFGRETA
                     RMIVRQPLELTKMGDKFDIMVTVEGGGISGQAGAIRLGVARALVEYDEALKSPLRKAG
                     FMTRDAREVERKKVGLHKARRATQFSKR"
     gene            1808..1881
                     /locus_tag="VFF05_10055"
     tRNA            1808..1881
                     /locus_tag="VFF05_10055"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1840..1842,aa:Gln,seq:ctg)
BASE COUNT          413 a          667 c          636 g          331 t
ORIGIN      
        1 ccaacctcat tgcggcaata tttcgccggc tcggaatgga cgcggtgaat aatcgaattc
       61 acggatggca tccgcattga cggcgatcaa gtcgatgcgc agacgcgcca gcgcgccggg
      121 cgcaattcgt gcgggtcgcg cgagcaggtg cagcccggtt agcggaatcg gcagcggcag
      181 cacgaatccc ggaaccgctt cgcgcacacg ccggagcatc gcgtcgaatc gcaggcgttc
      241 gccgccaccc agctcgtagc ccttgccgaa tgtcacgggt tgggaaatga cggcgtggca
      301 ggcggcagcg agatccgccg catgcacggg ctggcgcaga cccgatgccc cgaacggaac
      361 cggaagcacg cgccagcgcc gggcaaagcg cgcgagtggc gccagcgttt tgtcgatgcc
      421 ggcgccgtaa atcagggtcg ggcggaagat cgtccaggcg attgcgcgtc ggtcggcaat
      481 ttcgcgcacg cgcgcctcgg cgccgcgcag gcgctgggcc agcgcgcgtt cggcaggatc
      541 gggagaatcc tgcttggttt gcgcactcat cgaactcatc gcgacgatgc ggcgaagtgc
      601 cgctgccggc gagcgttcca gccagtcggc aaaagcgtcc agcgggccca ggctgaggac
      661 gatttcggcg tccggccaga tcgcggccgg atcgttcagg tcggcgcata tccagcccgg
      721 ccgggcctgg cggcttaccg gcagaacgcg atgatctggc gcgaacaggg gcaggacgaa
      781 gcgcccgatc gccccgctgg cgccgaaaac gaggatcgtg gacatggccg cactgtccgg
      841 cgcggcacgg gttcgagtca agggcagtat tgcacgccgc gccagcgtcc cgtatacttc
      901 gaagtctttc gtttcacgcc gcttccggca caggaattcc catgaaaacg atcagcgcga
      961 aagctgagac ggtgaagcgc gactggtatc tggtcgacgc cagcaacaag acgctcggcc
     1021 gcctggccag cgaactggcg cgccgcctgc gcggcaagca caagactgtg ttcaccccgc
     1081 acgtggacac cggcgactac ctcgtcgtga tcaacgccga gaagatcacc gccaccggca
     1141 acaagatgga cgacaagatc taccatcacg tcaccggcta tatcggcaac atcaagtcga
     1201 tcgcgctcaa ggatctgctc gtcaagcatc cggaacgcgc catcgagatc gccgtgcagg
     1261 gcatgctgcc gaagaatccg ctcggccgcg ccatgtaccg caagctcaag gtctacaagg
     1321 gcccgaacca tccgcatacg gcgcaacagc cgcaagtatt ggacatctga tcatggcaac
     1381 cacgcaaaac tacggcaccg gccgccgcaa atcctccgcc gcgcgcgtgt ttctgcgccc
     1441 gggcaagggc agcatcgtcg tcaacggcaa ggcgctggat gaatttttcg gccgcgaaac
     1501 cgcacgcatg atcgtgcgcc agccgctgga actgaccaag atgggcgaca agttcgacat
     1561 catggtcacc gtcgaaggcg gcggcatctc cggccaggcc ggtgcgatcc gccttggcgt
     1621 ggcgcgcgcc ctcgtcgaat acgacgaggc gctcaagtcg ccgctgcgca aggccgggtt
     1681 catgacccgc gacgcgcgcg aagtcgagcg caagaaagtc ggtctgcaca aagcccgccg
     1741 cgcgacgcag ttctcgaagc gctaattcga acttcggttc ggatctccaa cctgtcagag
     1801 agggttttgg gagatcgtct aacggtagga caacggactc tgactccgtt tatctaggtt
     1861 cgaatcctag tctcccagcc acaaaacaaa agcccttgag aaatcaaggg cttttgcgtt
     1921 ttatagcctt gaggcgcttg gtcaccggct tggtcaccac aagcccatgc gcctacctca
     1981 ccacctcgtt cgttccgctt ccggcctttt ccattttcgc ctgaaggtcc ccgccgattt
     2041 gcgcacc
//
LOCUS       DASRVO010000814         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_257725, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000814 DASRVO010000000
VERSION     DASRVO010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257725"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1929)
                     /locus_tag="VFF05_10060"
     CDS             complement(100..1929)
                     /locus_tag="VFF05_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52196.1"
                     /translation="MNANLRLRPSLTAALFCGFTAFSTSPQIRAQDASAVPHLLELFG
                     VLPAPTYDPSQSEIPRRRPGHPYQAPPEFKVINPNGIPPAPVDQAGAFIPVPDRWRIM
                     EALGFKFPWYDPYNQNTYKGDKPIDGKNHFLVLTGISDTFIEPRSVPTPVAPQSTGNP
                     GEQGVFGGINQLLLVQNFIASVDYYEGDTTFKPPDIEYKATLAFNYNRINTRETRAVN
                     IDPRLGTDRDDGFIGVQELWVDKHLRDVSSRYDFDSLRIGIQPFSTDFRGFLFQDSQP
                     GIRLFGNRDNNRWQYNFAYFRRLEKDLNSGLNDVTKSPRHDDILVANLYRQDWPWLGY
                     TSQATIVYNHNSDDTIYDSNGFIQRPAAIGLQLPRKYDVTYLGYNGDGHIGRVNLTVS
                     AYGAFGHQDHGVFVEPSTSIRAGFLAAEASMDFDWIRARLSAAYASGDKNAYDRRSSG
                     FDAINENPIFAGADTSYWISQAVPLIGGGGVALSQPNGMLADLRSSSALGQSNFDNPG
                     LRLLGIGADFDITPQSRVTGNLNELWFDNTATLQSLRQQSNIGRSIGTDASVAWTWRP
                     YFTQNVIVRLSGAALRPGSGFKSLFSSDHSLYYSVLANVILTY"
     gene            complement(1931..>3008)
                     /locus_tag="VFF05_10065"
     CDS             complement(1931..>3008)
                     /locus_tag="VFF05_10065"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZW52197.1"
                     /translation="AILTGTAFGQVASGIRPDDAFYPGLDAFVLDDGNGNNRYPPIAG
                     SDGANALTAQEVQTIVQQALTIANRARAQIRNPLGSQARVTISVVDTNGVALAVARTR
                     DAPVFGTDVSLQKARTAAFNSGSQAADVLLALAPANYFADNLDYANKKATFTTVAQSH
                     IGDYVSAVRTFLGIPDALTGGVAFTDRAGGNMSRPFFPDGLDSAPNGPFSHPFAQWSP
                     FQEGEQLDLVYNAIALNLVGYLGAQEFAISADNVPATTQFCSANPLLANGIQVFPGSV
                     PIYRGSTLVGGLGASGDGVDQDDMVTFLGLHNAGVTLNGKVNNAPPAMRADQIAPQGV
                     HLRYVQCPQSPFLDSNADNVCDGK"
BASE COUNT          506 a          856 c         1070 g          576 t
ORIGIN      
        1 atgaccggct gttcttcgtt ggcgctggcg agcgcgatcg gcagcaacat gacgactgcg
       61 gtgacgatga cccagttggc tttggtcatg gcaaggccct caataggtca aaatcacgtt
      121 cgccagcacc gagtagtaca gcgaatggtc ggagctgaac aggctcttga aaccggagcc
      181 gggtctgagc gcggcgccgg aaaggcgcac gatcacgttc tgggtgaagt agggccgcca
      241 tgtccacgcg acggatgcat cggtgccgat gctgcgaccg atgttcgatt gctggcgcag
      301 cgattgcagc gtcgccgtgt tgtcgaacca caactcgttg aggttgccgg ttacgcgcga
      361 ttgcggcgtg atgtcgaaat cggcgccgat gccgagcagg cgcaggccgg gattgtcgaa
      421 attcgattgg ccgagcgcgc tcgacgagcg cagatccgcc aacatgccgt tgggctggct
      481 cagcgccacg ccgccgccgc cgatcaacgg cacggcctgg ctgatccagt aacttgtgtc
      541 ggcgccggcg aagatcgggt tttcgttgat cgcgtcgaat ccgctcgaac ggcggtcgta
      601 ggcattcttg tcgccgctcg cgtaggccgc ggacaggcgt gcgcgaatcc agtcgaaatc
      661 catcgacgcc tcggcggcga ggaaacctgc gcggatgctc gttgatggct ccacgaacac
      721 gccgtgatcc tgatgcccga acgcgccgta agcggacacg gtgagattga cgcggccgat
      781 gtggccgtcg ccgttgtagc ccagataggt cacgtcgtac ttgcgcggca actgcaggcc
      841 gatcgccgcc gggcgctgga tgaaaccgtt ggaatcgtag attgtgtcgt cggaattgtg
      901 gttgtagacg atggtcgcct gcgaggtgta gccgagccac ggccagtcct ggcggtacag
      961 gttggcgacg aggatgtcgt cgtggcgcgg cgacttcgtc acgtcgttca ggccggaatt
     1021 gagatccttc tccaggcgcc ggaagtaggc gaagttgtac tgccagcggt tgttgtcgcg
     1081 gttgccgaac aggcgtatgc cgggctgcga atcctggaac aggaaaccgc ggaaatcggt
     1141 cgagaacggc tggatgccga tgcgcagcga atcgaaatcg tagcgcgagg aaacatcgcg
     1201 caggtgcttg tcaacccaca gttcctgcac gccgatgaag ccgtcgtcgc ggtcggtgcc
     1261 caggcgtggg tcgatgttca ccgcacgcgt ctcgcgcgtg ttgatgcggt tgtagttgaa
     1321 cgcgagcgtg gccttgtatt cgatatcggg cggcttgaac gtggtgtcgc cttcgtagta
     1381 gtccacgctg gcgatgaagt tttgcaccag caacagctgg tttattccgc cgaacacgcc
     1441 ctgttcgccc gggtttccgg tgctctgcgg cgcgactggc gtcggcacgc tgcgcggttc
     1501 gatgaaggtg tcggaaatgc cggtgaggac gaggaaatgg tttttcccgt cgatgggttt
     1561 gtcgcccttg tacgtgttct ggttgtacgg gtcgtaccag gggaacttga aaccgagcgc
     1621 ttccatgatg cgccagcggt cgggcaccgg gatgaaggcg ccggcctggt ccaccggtgc
     1681 gggcgggatg ccgttcggat tgatgacctt gaactccggc ggcgcctggt agggatgacc
     1741 ggggcgccgg cgcgggattt ccgactggct cggatcgtag gtcggtgctg gcagcacgcc
     1801 gaacaattcc agcaggtgcg gaacggcgct cgcatcctgc gcgcggattt gtggacttgt
     1861 ggaaaacgcg gtaaacccgc agaacagggc agcggtgagg gatggacgca ggcggagatt
     1921 cgcgttcatg tcatttccca tcgcagacgt tgtcggcgtt gctgtcgagg aatggcgact
     1981 gcgggcattg cacgtagcgc aggtgcacgc cctgcggcgc gatttggtcc gcgcgcatcg
     2041 cgggtggggc gttgttcacc ttgccgttca gcgtcacgcc ggcgttgtgc aggccgagga
     2101 aggtgaccat gtcgtcctgg tccactccgt cgccggaggc tccgaggccg ccgaccaagg
     2161 tgctgccgcg gtagatcggc acgctgccgg gaaagacctg gatgccattc gctagcaatg
     2221 gattggcgga acagaattgg gtcgtggcgg gcacgttgtc ggcgctgatc gcgaattcct
     2281 gcgcgccgag atagccgacc aggttcagag cgatcgcgtt gtagaccagg tcgagctgct
     2341 cgccttcctg gaacgggctc cattgcgcaa acggatggct gaacggaccg ttcggggcgc
     2401 tgtcgaggcc gtcgggaaag aacggccggg acatgttgcc gccggcacgg tcggtgaatg
     2461 cgacgccgcc ggtaagcgcg tccgggatgc cgaggaaggt acgcacggcg ctgacgtaat
     2521 cgccgatgtg gctttgcgca acggtcgtga acgtcgcttt cttgttggcg tagtcgaggt
     2581 tgtcggcgaa gtaattcgcc ggcgccagcg ccagcagcac gtcggccgcc tggctgccgg
     2641 aattgaatgc cgccgtgcgc gccttttgca gggagacgtc cgtaccgaac accggcgcat
     2701 cgcgcgtgcg cgccaccgcg agcgccacgc cgttcgtgtc gacgacggaa atagtcacgc
     2761 gcgcctggct gccgagcggg ttgcggatct gtgcgcgcgc ccgattcgcg atcgtcagtg
     2821 cctgttgcac gatggtctgt acttcctgcg cggtcagcgc gttcgcgccg tcgctgccgg
     2881 cgatgggcgg atagcggttg ttgccattgc catcgtcgag gacgaacgca tcgagccccg
     2941 ggtagaaggc gtcgtccggg cggatgccgg aagcgacctg gccgaaggcc gtgccggtga
     3001 ggatcgcg
//
LOCUS       DASRVO010000815         1105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_76306, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000815 DASRVO010000000
VERSION     DASRVO010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76306"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1105)
                     /locus_tag="VFF05_10070"
     CDS             complement(<1..>1105)
                     /locus_tag="VFF05_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZW52198.1"
                     /translation="MVARLTRHTDPAFLHYRSGAFMAERFRKLPGSDGRRLDPIMDSA
                     LSFAASKDDPASGGRHKVWGSKPLWVLPQTSTIASHLPKAFGTAVAIEQARRIGHKLP
                     IPDDSIAICSFGDASSNHATAQTAFNAAGWTAYQKLPAPVLFVCEDNGIGISVKTPND
                     WIANNFRDRKDLDYFYADGLDLAAGYEGVARAIHHCRTTRRPTFLHLRTTRIMGHAGT
                     DFEIEWRSVEELMAVEATDPLLRSAAIALVAGVCTKEQLLARYEAIRKKCFAAAEEAD
                     RRPKLTDLHEVMAPLAPYHPNQVALEARRADFAEKRLAVFGSAEKLPEKLPPRHLAIQ
                     INQALHDLFCKYPEALLFGEDVAQKGGVYTVTKG"
BASE COUNT          183 a          351 c          369 g          202 t
ORIGIN      
        1 cccttggtga cggtatacac gccgcccttc tgcgccacgt cctcgccgaa cagcaatgcc
       61 tcgggatact tgcagaacaa gtcgtgcagg gcttggttga tctggatcgc caggtgccgc
      121 ggtggcaatt tttccggcaa tttttccgcc gacccgaaaa cggcaaggcg cttttccgcg
      181 aaatccgcgc gccgggcttc cagagcgacc tggttcggat ggtaaggcgc caacggcgcc
      241 atcacctcgt gcaggtcggt caacttgggc cggcggtcgg cttcctcggc cgcggcgaaa
      301 cacttcttgc gaatggcttc gtagcgtgcg agcagttgtt ccttcgtgca tacgccggca
      361 acgagcgcga ttgccgccga gcgcagcagc gggtcggtcg cctcgaccgc catcaattcc
      421 tcgacactgc gccattcgat ctcgaaatcg gtgccggcat gacccatgat gcgcgtggtg
      481 cgcagatgca ggaacgtcgg ccggcgcgtg gtgcgacagt ggtggatcgc gcgcgccacg
      541 ccttcgtagc cggcggcaag atcgaggccg tcggcgtaga aataatccag gtccttgcgg
      601 tcgcggaaat tgttcgcgat ccaatcgttc ggcgtcttca ccgagatgcc gatgccgttg
      661 tcctcgcaca cgaaaagcac tggcgccggc agtttctggt aggccgtcca gcccgcggca
      721 ttgaacgcgg tctgcgcggt ggcgtggttg ctggaggcgt cgccgaacga gcagatcgcg
      781 atggaatcgt ccgggatcgg cagcttgtgg ccgatgcgcc gcgcctgctc gatcgccacc
      841 gccgtgccga acgccttggg caggtgcgag gcgatggtcg aggtttgcgg cagcacccac
      901 agcggcttgc taccccagac cttgtggcgc ccgcccgagg ccgggtcgtc cttgctcgcg
      961 gcgaaggaca gcgccgaatc catgatcgga tccaaccgtc ttccatcaga gccgggcagc
     1021 ttgcggaagc gttcggccat gaacgcgccg gagcgataat gcaggaaggc cgggtcggtg
     1081 tgccgcgtca gccgcgcgac catgg
//
LOCUS       DASRVO010000816         1404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_360941, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000816 DASRVO010000000
VERSION     DASRVO010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360941"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFF05_10075"
     CDS             <1..342
                     /locus_tag="VFF05_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZW52199.1"
                     /translation="LVNGVKAGCEVWPHAARPADFHTPLKTDKPVLILEGALDPVTPP
                     RYGEQVVKNLSNARLIVAKGQGHNVIGRGCIPKLVGDFVDKLDPKTMDAKCVDRLGPM
                     PAFVNFNGAAP"
     gene            339..1085
                     /locus_tag="VFF05_10080"
     CDS             339..1085
                     /locus_tag="VFF05_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZW52200.1"
                     /translation="MIEVRNLHKAFGKGAKRVVAVDGVSFSARDGEITGLLGPNGAGK
                     TTTLRCLYTLMQPDSGQVLVDGIDAAVDPVAVRRRLGVLPDARGLYKRLTARENIDYF
                     ARLHGIETQTMHQRREALADALEMRDILDRRTEGFSQGQRVKTAIARALVHDPKNVIL
                     DEPTNGLDVMSTRAMRGFLKSMKAEGRCVLFSSHIMQEVAALCDRIVVIARGRVVADE
                     SPDALRAQTGEANLEEAFVKLIGTDEGLAA"
     gene            1082..>1404
                     /locus_tag="VFF05_10085"
     CDS             1082..>1404
                     /locus_tag="VFF05_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZW52201.1"
                     /translation="MNSVITVFLKEVRENLRDRRTVINTLFTGPLIAPLLFVLMINVM
                     VTRELNKAEAPLPLPVAGAQYAPNLIAALKQNNVEIKDAPADAERAVREQDADVVLRI
                     DADFA"
BASE COUNT          273 a          449 c          449 g          233 t
ORIGIN      
        1 ctcgtcaacg gcgtcaaggc cggctgcgag gtctggccgc acgcggcgcg gccggccgat
       61 ttccacacgc cgttgaaaac cgacaagccg gtgctgatcc tcgaaggcgc actcgatccg
      121 gtcacgccgc cgcgctacgg cgagcaggtg gtgaagaatt tgagcaacgc gcgcctgatc
      181 gtcgccaagg gccagggcca caacgtgatc ggccgcggct gcatcccgaa actggtcggc
      241 gatttcgtcg acaagctcga tccgaaaacg atggatgcga aatgcgtcga ccggctcgga
      301 ccgatgccgg cgttcgtgaa tttcaatgga gccgcgccat gatcgaagta agaaatctgc
      361 acaaggcctt cggcaagggc gccaagcgcg tggtcgccgt ggacggcgtt tcgttcagtg
      421 cgcgcgatgg cgagatcacc ggcctgctcg ggccgaacgg cgccggcaag accactacgt
      481 tgcgctgtct gtatacattg atgcaacccg attccgggca ggtgctggtc gatggcatcg
      541 atgccgcagt cgatccggtc gcggtgcggc ggcgcctggg cgtgctgccc gacgcgcgcg
      601 gcttgtacaa gcgcctgacc gcacgcgaga acatcgatta tttcgcgcgc ctgcacggca
      661 tcgagacgca aacgatgcac caacgccgcg aagcgctcgc cgacgcgctg gaaatgcgcg
      721 acatcctcga ccgccgcacc gaaggctttt cgcagggcca gcgcgtcaag acggcgatcg
      781 cgcgcgcgct ggtgcacgat ccgaagaatg tcatcctcga cgagccgacc aatggcctgg
      841 atgtgatgtc cacgcgcgcc atgcgcggct tcctcaagtc gatgaaagcc gaaggtcgct
      901 gcgtgctgtt ctccagccac atcatgcagg aggtagccgc gttgtgcgat cgcatcgtgg
      961 tgatcgcgcg cggccgcgtc gtcgccgacg aatcgccgga tgcgctgcgc gcgcaaacgg
     1021 gcgaggccaa tctcgaagag gcgtttgtaa aactgatcgg caccgatgaa ggactcgccg
     1081 catgaattcc gtcattaccg tattcctcaa ggaagtccgc gagaacctgc gcgatcgccg
     1141 caccgtgatc aacacgctgt tcaccggtcc gctgatcgcg ccattgctgt tcgtcctgat
     1201 gatcaacgtc atggtcacgc gcgagctgaa caaggccgag gcgccactgc ccctgccggt
     1261 ggccggtgcc cagtacgcgc ccaacctgat cgccgcactc aagcaaaaca atgtcgagat
     1321 caaggatgcg ccggctgacg ccgaacgcgc ggtgcgtgaa caggatgccg acgtggtgct
     1381 gcgcattgat gccgatttcg ccaa
//
LOCUS       DASRVO010000817         1351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_257788, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000817 DASRVO010000000
VERSION     DASRVO010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257788"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..>1351)
                     /locus_tag="VFF05_10090"
     CDS             complement(137..>1351)
                     /locus_tag="VFF05_10090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZW52202.1"
                     /translation="LTGSIGRPLLGIASEMFGAQRVYALLMLVVAGFTFALPYAHSYF
                     ALLLLGLGFGLAGASFAVGVVYVSAWYPRERQGTALGLFGIGNVGAALTSLAAPQLLS
                     FMDWRHVADVYGVLVLATAALFFMFAQTDPLTGKRAGAGRPATLAERLSPLHNLQVWR
                     FSLYYFLVFGGFVALASWLPRYYMGVYKVDLRTAGLLAASFALPATLFRAVGGVLADR
                     FGARRMMYISFSVCMVGLFLLSYPPTHYVIDGIRGPIAFTIAPSMFERASVLFLLGVV
                     MSFGMAAVYKHIPTYYPDHVGAVGGVVGMIGGLGGFFLPIAFGAMNDLVSVWSSCFML
                     LFVIALTSLLWMHFAILRMNRMRHPGLKADTDLPEIMAVRDAVLRAREAAEAASRAAQ
                     QAAAAAARLRAG"
BASE COUNT          287 a          445 c          446 g          173 t
ORIGIN      
        1 agcctggatt tcctcggcct gctgccgcac gtgatgttcg gcattggcct cgcggcctgg
       61 gcgatccttt tcgtcggcca gttgcgcgcg ttgcgccgac agtggcacga gtccggcgcg
      121 acctgagtcc ggcgcatcag ccggcgcgca accttgccgc cgcggcagcc gcctgctgcg
      181 cggcgcgact ggccgcttct gcggcttccc ttgcgcgcag cacggcgtcg cgcactgcca
      241 tgatttccgg aaggtcggtg tcggccttga gtcccggatg acgcatgcgg ttcatgcgca
      301 ggatcgcgaa gtgcatccac aacaggctgg tcagcgcaat cacgaacagc agcatgaagc
      361 aactactcca gacgctcacg aggtcgttca tcgcgccaaa cgcaatcggc agaaagaagc
      421 cgccgaggcc accgatcatg ccgaccacgc cgccgaccgc gccgacatga tcgggataat
      481 aggtcggaat gtgcttgtac acggcggcca tgccgaagga catgaccacg ccgagcagga
      541 acagcacgga tgcgcgctcg aacatgctcg gcgcgatcgt gaatgcgatc ggcccgcgga
      601 tgccgtcgat cacgtaatgc gtgggcggat acgagaggag gaacaggcca accatgcaca
      661 ccgagaacga gatgtacatc atgcgccgcg cgccaaagcg gtcggcgagc acgccgccga
      721 cggcgcggaa caaggtcgcc ggcagggcga aactcgcagc aagcagacct gcggtgcgca
      781 ggtcgacctt gtacacgccc atgtagtagc gcggcagcca cgaagccagc gccacgaagc
      841 cgccgaacac gaggaagtag tacagcgaga atcgccacac ttgcaggttg tgcagcggcg
      901 agagacgttc ggccaacgtg gccggccggc ccgcgccggc gcgcttgccc gtcagcggat
      961 cggtttgcgc aaacatgaag aacagcgcgg cggtcgcgag cacgagcacg ccgtagacat
     1021 cggccacatg gcgccaatcc atgaacgata gcagctgcgg cgcggcgagg ctggtgagcg
     1081 ctgcgccgac attgccgatg ccgaacaggc cgagcgcggt gccctggcgc tcgcgcggat
     1141 accaggccga cacatagacg acgccgaccg cgaacgaagc tccggccagg ccgaaaccaa
     1201 gaccgagcaa gagtagcgcg aagtaggaat gcgcatatgg cagcgcgaac gtgaacccgg
     1261 ccacgaccag catcaacagc gcatacacac gctgcgcgcc gaacatctcc gaagcgatgc
     1321 cgagcagcgg ccggccgatc gagccggtca g
//
LOCUS       DASRVO010000818         1964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_51106, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000818 DASRVO010000000
VERSION     DASRVO010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51106"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFF05_10095"
     CDS             complement(<1..281)
                     /locus_tag="VFF05_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZW52203.1"
                     /translation="MSLQAWAVAAIVIAAMALFVSEKLRYDVVGLLVLVALLVSGILT
                     PAEGLSGFSNEATIVVAAMFALNAGLVGTGALDPLIRTLSRIQRPWLLTL"
     gene            complement(278..>1964)
                     /locus_tag="VFF05_10100"
     CDS             complement(278..>1964)
                     /locus_tag="VFF05_10100"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HZW52204.1"
                     /translation="LADTAFKQEIRLSQRWTDCHGREHGEMIGRPVSMHAMRGIAAHA
                     NGFHTCRMLHVLQMLLGAIDTPGSFRYQPPYPKSVPPANRPGKSRKDNGALNAGPLGY
                     VHAPEDLVVDAHGAPRRIDKAFSWEFPLAAHGMLQSVIRNACAGDPHKIDTLFLFMAN
                     MGWNSAMNTRDTQRMLCDKDADGAYKIPRFIYADAYWSETVNYADLVLPDTTYLERHD
                     CISLLDRPISDADAAADAIRQPVCAPDRDVRPFQDVLLDLGTRLQLPGMVDADGAAKY
                     PRGYAQYMVEHERAPGVGLLAGWRGQSGDKPGIGAPNPGQLDAYKAHDCHWETKIPDA
                     GRYYKMANRDYLAWAKSIGFVGSTDAIVLEIYSERLQKFRLAAQGHGAVQPPARERAR
                     VEKFFDPLPYWYATASDDAAFPVSAITQRPMFMYHAWGSQNAWLRQIAARNFLYLHPD
                     TAQAHAIADGDWMFVESAHGRIRVQAKFHEGTAPGVVWTWNAIGKQRGAWKLDANAPE
                     YTKGFLLNHAIDDLLPPRADGYRYANADPVTGQAAWFDLRVRIARADATEQAA"
BASE COUNT          332 a          624 c          665 g          343 t
ORIGIN      
        1 agcgtcagca gccacggccg ctggatgcgc gacagcgtgc gaatcagagg gtcgagtgcg
       61 ccggtgccga cgagtcccgc gttcagcgcg aacatcgccg cgacgacgat cgtggcctcg
      121 ttgctgaagc cggacaagcc ttccgccggc gtgaggattc ccgacacgag cagggcgaca
      181 agcacgagca ggccgaccac gtcgtagcgc agcttctcgc tgacgaacag cgccatcgcg
      241 gcgatcacga tggcggcgac ggcccaggct tgcaggctca tgcggcttgc tccgtggcgt
      301 cggcgcgcgc gatgcgcacg cgcaaatcga accacgccgc ctgtccggtc acgggatccg
      361 cgtttgcgta gcggtagccg tcggcgcgcg gcggcagcag gtcgtcgatc gcgtggttga
      421 gcaggaatcc ctttgtgtac tccggcgcat tcgcgtcgag cttccacgcg ccgcgttgct
      481 tgccgatcgc gttccaggtc caaaccacgc ccggcgcggt gccctcgtgg aatttcgcct
      541 gcacgcggat gcggccatgc gcggactcga cgaacatcca gtcgccgtcg gcgatggcgt
      601 gcgcctgtgc ggtatcggga tgcaggtaca ggaagttgcg cgccgcaatc tggcgcagcc
      661 acgcgttctg cgaaccccag gcgtggtaca tgaacatggg gcgttgggtg atggcggaaa
      721 ccggaaacgc cgcgtcgtca gacgcggtcg cgtaccaata gggaagcgga tcgaagaatt
      781 tttccacgcg cgcacgctcg cgcgccggcg gctgcacggc gccgtgcccc tgcgcggcga
      841 ggcggaattt ctgcaggcgc tcggaataga tttccagcac gatcgcgtcc gtcgaaccga
      901 cgaagccaat cgacttggcc caggcaagat aatcgcgatt ggccatcttg taatagcggc
      961 cggcgtcggg aatcttcgtt tcccaatggc aatcgtgcgc tttgtaggcg tcgagttgac
     1021 ccggattcgg tgcgccgatc cccggcttgt cgccagattg accgcgccag ccggcgagca
     1081 ggcccacgcc cggcgcgcgc tcgtgctcga ccatgtactg cgcatagccg cgcggatatt
     1141 tcgccgcacc atccgcatcg accatgccgg gcaattgcag acgcgtgccg agatcgagca
     1201 gcacgtcctg gaatgggcgc acgtctcgat ccggcgcaca caccggctgg cggatcgcat
     1261 ccgcggcggc gtcggcgtcg gaaatcggcc gatccaacag cgagatgcag tcgtgacgtt
     1321 ccagataggt cgtatccggc agcaccaggt cggcgtagtt caccgtctcc gaccagtagg
     1381 cgtcggcgta gatgaaacgc ggaatcttgt acgcgccgtc ggcatccttg tcgcaaagca
     1441 tgcgctgcgt gtcgcgcgtg ttcatcgccg agttccagcc catgttcgcc atgaacagga
     1501 acagcgtgtc gatcttgtgc ggatcgccgg cgcaggcgtt gcggatcacg gattgcagca
     1561 tgccgtgtgc ggcgagcggg aattcccagg agaacgcctt gtcgatgcgg cgcggcgcgc
     1621 cgtgcgcgtc gacgacgagg tcttcgggcg cgtgcacgta gccgagtggt ccggcattga
     1681 gtgcaccgtt gtcctttctc gatttgccgg gccgattcgc cggcggcacg gatttcggat
     1741 acggcggctg gtagcggaaa ctccccggcg tgtcgatggc gccgagcagc atctgcaaaa
     1801 cgtgcaacat gcggcaggtg tggaagccgt tcgcgtgtgc ggcgatgcca cgcatcgcgt
     1861 gcatggatac cggccgaccg atcatctcgc cgtgttcgcg accgtggcaa tccgtccagc
     1921 gttgcgacag acgaatttcc tgcttgaacg ccgtgtcggc gagt
//
LOCUS       DASRVO010000819         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_76393, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000819 DASRVO010000000
VERSION     DASRVO010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76393"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /gene="pxpB"
                     /locus_tag="VFF05_10105"
     CDS             <1..458
                     /gene="pxpB"
                     /locus_tag="VFF05_10105"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HZW52205.1"
                     /translation="FDHVAEHLRTLVDNAASDAATDTGNIEIAVTYGGQCGPDLADVA
                     AHTGLSEEEVVARHGAAEYRVAMLGFTPGFAYLLGLDPALHMPRRATPRTRVPAGSVA
                     IGGAQTGVYPRETPGGWHLIGRTTASLFDPARDPPALLKPGMRVKFVAR"
     gene            455..1447
                     /locus_tag="VFF05_10110"
     CDS             455..1447
                     /locus_tag="VFF05_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HZW52206.1"
                     /translation="MSISVLAPGWQTSVQDGGRHGFTALGVGASGAMDDVSRRLANIL
                     VGNVENAATLEITLRGPRLRFDADCAVALTGAALDGLPMWRPVAVKAGTEIAFGTMAR
                     GAYAYLAVAGGIGGDKIIASRSADIGAGLGQALAAGDVLPLANESRLAHRELWQRFDS
                     SQPPPGTDNPGVVSAKWSLNPRPWFELDPNNPIGMVEGAHFTQLSDSARHTLFHATFR
                     IGGDSSRMGLRLREARLELRAPLELVSAAVVPGTVQLPPSGDPIVLMAEAPTCGGYPR
                     IGHIPTVELPRLAQRRPGDSVRFAPMSLGAAQTRYLERERALAALARTIKDRLQ"
     gene            1444..2205
                     /locus_tag="VFF05_10115"
     CDS             1444..2205
                     /locus_tag="VFF05_10115"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HZW52207.1"
                     /translation="MTRIDLNCDMGESFGAWTMGDDAGVMPWIASANIACGFHAGDFS
                     TMRKTVLLAKQHGVAIGAHVSLPDLQGFGRREMKILADDTYALTLYQIGALSAFARVA
                     GRRVAHVKPHGALYNMAAKDAVLADAVARAVRDFDAQLIVVGLAGSELPKAGAALGLA
                     VAHEAFADRRYEADGSLTPRSVGGAVIEDADAAVAQAVQIATKGKVDIRTGGTLDLHA
                     DTICVHGDRPDAALFARKLREGLKAAGVEDAKFAG"
     gene            complement(2202..2573)
                     /locus_tag="VFF05_10120"
     CDS             complement(2202..2573)
                     /locus_tag="VFF05_10120"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HZW52208.1"
                     /translation="MGKPSDEFTRFVLDQLARLPTLTSAPFFGGIGLKSGASFFGMIM
                     DGALYFSTSPATRAEYEKHGSRCFSYAKQGKVQATKLFEVPAEVLDNADTLRDWAQRA
                     IADAAKKRKVGALRKRKQGNR"
     gene            complement(2599..2946)
                     /locus_tag="VFF05_10125"
     CDS             complement(2599..2946)
                     /locus_tag="VFF05_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HZW52209.1"
                     /translation="MKHKGSCHCGRIAFEVEGEIKGATACNCSICQRKGSLMWFVPKS
                     AFHLLTPDDAASTYTFNKHRIKHRFCPVCGMHPYGEGADSKGSEMAAINIRCLEDIDL
                     KAIPVTEFDGRSL"
     gene            complement(2956..>3290)
                     /locus_tag="VFF05_10130"
     CDS             complement(2956..>3290)
                     /locus_tag="VFF05_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZW52210.1"
                     /translation="VFVLTHHARAPLAMKGGTTFYFVTDGIESALAQAREAANGRDVR
                     LGGGAATIREYLRARLIDELHLAVRPVLLGSGEALFSGLDWRALGYECTKSVAGERAM
                     HVFMSRRV"
BASE COUNT          533 a         1112 c         1077 g          568 t
ORIGIN      
        1 ctttcgatca cgtcgccgaa cacctgcgta cacttgtgga caatgccgca tccgatgcgg
       61 cgacggacac gggcaacatc gagattgcgg tgacgtacgg cggccaatgc ggacccgacc
      121 tcgccgatgt cgccgcgcac accgggttgt ccgaagaaga agtcgtggcg cgccacggcg
      181 cagcggaata ccgcgtcgcc atgctcggct tcacgcccgg gtttgcgtat ctgctcggcc
      241 tggacccggc cttgcacatg ccgcggcgcg cgaccccgcg cacgcgcgtg ccggccgggt
      301 ccgtcgccat cggcggcgca cagaccggcg tgtatccgcg cgaaacgccg ggcggctggc
      361 acctgatcgg acgcacgact gcgagcctgt tcgatcctgc gcgcgatccg ccggccctgc
      421 tcaaaccggg catgcgcgtg aaatttgtcg ctcgatgagt atttccgttc tcgctcccgg
      481 ctggcagacc agcgtgcagg atggcggccg ccacggcttc acggcactgg gcgtcggcgc
      541 gtccggcgcg atggacgatg tctcccggcg gctcgcgaat atacttgttg gcaatgtgga
      601 gaatgcggca acattggaaa tcaccttgcg cggtccgcgc ctgcgcttcg acgcggattg
      661 cgcggtcgcg ctcaccggcg ctgcgctcga cggcctgccg atgtggcgcc ccgtggccgt
      721 caaagcggga acggaaatcg ctttcggcac gatggcgcga ggcgcgtatg cctaccttgc
      781 ggttgccggc ggcatcggcg gcgacaagat catcgccagc cgcagcgccg atatcggcgc
      841 cgggctcggg caggcgctcg cggcaggcga cgtgctgccg ctcgcgaacg agtcgcgcct
      901 ggcacaccgc gagttgtggc agcgcttcga ctcaagccag ccgccgccag gcaccgacaa
      961 tcccggcgtg gtttcggcga agtggtccct caatccgcgc ccgtggttcg agctggatcc
     1021 gaacaatccc atcggcatgg tcgaaggcgc gcatttcacg caactgagcg actccgcccg
     1081 gcacacgcta ttccatgcga cgttccgcat cggcggcgat tccagtcgca tgggcctgcg
     1141 cctgcgcgag gcacggctgg aactgcgcgc gccactggaa ctggtttccg ccgccgtcgt
     1201 tcccggcacc gtgcaactgc cgcccagcgg cgatcccatc gtgctgatgg ccgaggcgcc
     1261 gacctgcggc ggctatccgc gcatcggcca cattcccacc gtcgagctgc cgcggctcgc
     1321 gcaacgccgg cccggcgaca gcgtgcgctt tgcgccgatg tcgctcggtg ccgcgcaaac
     1381 gcgctatctt gagcgcgaac gcgcgctggc ggcgctggcg cgaaccatca aggatcgcct
     1441 gcaatgaccc gcatcgacct caactgcgac atgggcgaat ccttcggcgc ctggaccatg
     1501 ggcgacgacg ccggcgtgat gccgtggatc gcttcggcaa acatcgcttg cggcttccat
     1561 gccggcgatt tttccacaat gcgcaaaaca gtactcctgg ccaaacaaca cggcgtggcc
     1621 atcggcgcgc atgtgtcgct gccggatttg cagggcttcg gacggcgcga aatgaaaatc
     1681 ctggcggacg acacctacgc gctgacgctc taccagatcg gcgcactgtc ggctttcgcg
     1741 cgcgtcgcgg gcaggcgcgt cgcgcatgtg aagccgcacg gcgcgctgta caacatggcc
     1801 gcgaaagacg cggtgctggc cgatgccgtc gcgcgtgcgg tgcgcgactt cgatgcgcag
     1861 ctgatagtcg tcggactcgc cggcagcgaa ttgccgaaag ccggcgccgc gctgggcctc
     1921 gccgtcgcac acgaagcctt cgccgaccgc cgctacgagg ccgatggttc gctcacgccg
     1981 cgcagcgtgg gaggcgccgt cattgaggat gccgatgccg cggtcgcgca ggcggtacag
     2041 attgccacaa agggaaaagt ggatatccgc accggcggca cgctcgacct gcacgccgac
     2101 accatctgcg tgcatggcga ccgccccgat gcggcgctgt ttgcgcgcaa gctgcgcgaa
     2161 ggattgaaag cggcgggcgt ggaagacgcg aaattcgccg gctagcggtt gccttgtttg
     2221 cgcttgcgca aagccccgac tttgcgcttc ttggccgcat cggcgatcgc gcgctgcgcc
     2281 cagtcgcgca gcgtgtcggc attgtcgagc acctcggccg ggacctcgaa cagtttcgtc
     2341 gcctggacct tgccctgctt ggcgtaggaa aaacagcggc tgccgtgctt ttcgtattcg
     2401 gcgcgcgtcg ccgggcttgt cgagaaatac agcgcgccgt ccatgatcat gccgaagaag
     2461 ctcgcgccgg atttgagccc gatgccgccg aagaaaggcg ccgacgtcag cgtcggcaaa
     2521 cgtgcgagtt gatcgagcac gaagcgggtg aactcgtcgc tgggtttgcc catgcgtacc
     2581 gactccgctc tggattcgtc agagcgaacg cccgtcgaat tccgttaccg ggattgcctt
     2641 caagtcgatg tcttcgaggc agcggatatt gatcgccgcc atttcgctgc ctttggagtc
     2701 cgcgccttcg ccatacgggt gcatgccaca gaccgggcaa aatcgatgct tgatccggtg
     2761 cttgttgaac gtgtacgtgc tcgcggcgtc atccggcgta agcagatgga acgcgctctt
     2821 gggcacgaac cacatcagcg agcccttgcg ctggcagatg gagcagttac acgccgtcgc
     2881 gcccttgatc tcgccctcga cttcgaatgc aatcctgccg cagtggcagc tgcctttgtg
     2941 tttcatgttg gctccttaca cgcgtcggct catgaaaaca tgcatcgccc tttccccggc
     3001 gacgctcttc gtgcattcgt aacccagcgc gcgccagtcc agccccgaga acagcgcttc
     3061 gccgctgccg agcagcaccg ggcgcacggc gaggtgcagc tcgtcgatca ggcgtgcgcg
     3121 caggtattcg cgaatcgtcg ctgcgccgcc gccgaggcgc acgtcgcggc cgttcgcggc
     3181 ctcgcgtgct tgcgcgagcg cggattcgat gccgtcggtg acgaagtaaa acgtcgtacc
     3241 gcctttcatc gccagcggcg cacgcgcgtg gtgcgtgagc acgaacacgg
//
LOCUS       DASRVO010000820         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_128929, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000820 DASRVO010000000
VERSION     DASRVO010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128929"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1980
                     /locus_tag="VFF05_10135"
     CDS             <1..>1980
                     /locus_tag="VFF05_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZW52211.1"
                     /translation="QRDAEAKLAEWQTQWDTYAKSSGEASKEAEVERTRLDYLDKQAL
                     ETARRLETLQTEKRGADFDALTEQLAKLSAEHDTHKQSVESHTRLLDERRTVHEQVLE
                     TERANATALNEARTRLQALRGRLASLEALQHAALGQEKSAASEWLAKLGLDKSRRLGE
                     VLQVEAGWETAVETVLGGLLEGVLAEAPHDHAGDLAGVHEADLALIATLEAGTAPTGT
                     LAAQVRGPQAALAILSQVRVAQSLDEARRIAANMAAHESVITQAGEWLGTGYARVLRR
                     GGAQAGMLAREREIQNLRGEIVQAQARVDQLAAEIDAIKLRKSEAEGARDDAQRELYT
                     AHRRVAELAGQIQSHSGRIETARTRVVKIDEEIAALQARTQTDEAATREARARLEQAV
                     ARMGEEEQRRQHLDGERRRLLEAREEARMNAREAGEQAHQHALGIESKRTGVAALEQA
                     LLRMSSQFAQLEARKAEIAQQLESSQAPLAGIEAERQTYLNQRLLVDKQLVEARKGVE
                     ERDNEFRGYETERHRIEHVLSQHREAHAERRLTHQSLQMRAEQLQEAIAAAGHELQAV
                     LDALPAGADPAEWQQQLEALDAKIKRLEPVNLAAISEFEEQSQRKTYLDAQLTDLNTA
                     LETLEGAIKKIDRETRQRFKETFDRVNTGLQELFPR"
BASE COUNT          426 a          649 c          659 g          246 t
ORIGIN      
        1 cagcgcgatg ccgaggcgaa actcgccgag tggcagacgc agtgggacac ctatgccaag
       61 tcttcgggcg aggcgtcgaa agaagccgaa gtcgaacgca cgcgcctgga ctatctcgac
      121 aagcaggcgc tggagacggc acgtcggctg gaaaccctgc aaacggaaaa gcgcggtgcg
      181 gatttcgatg cactgaccga gcaactcgcc aagctcagcg ccgaacacga tacgcataaa
      241 cagtccgtcg aatcgcatac gcgcctgctc gacgaacgtc gcaccgttca cgaacaggtg
      301 ctggaaaccg aacgcgcgaa cgccaccgca ttgaacgaag cacgcactcg cctgcaggct
      361 ctgcgcggca ggctggcttc gctggaagcg ttgcagcatg cggcgttggg ccaggaaaaa
      421 tccgcagcga gcgagtggct ggccaaactg ggcctggaca agtcgcgccg cctcggcgaa
      481 gtgctgcaag tcgaagcggg ctgggaaaca gccgtcgaga ccgtgcttgg cggcctgctc
      541 gaaggcgtgc tcgccgaagc gccgcacgat catgcgggcg atttggccgg cgtgcacgaa
      601 gccgatctgg ctctgatcgc gacgcttgaa gccggcaccg cgccgacagg gacgctcgcc
      661 gctcaggtgc gcgggccgca ggcggcactt gccatccttt cccaggtgcg tgtggcgcaa
      721 tcgctggacg aagcgcgccg catcgcggca aatatggccg cgcacgagtc ggtgatcacg
      781 caggccggcg aatggctggg caccggctac gcgcgcgtgc tgcggcgtgg cggcgcgcag
      841 gcgggcatgc tcgctcgcga gcgtgaaatc cagaatctgc gcggcgagat tgtgcaggcg
      901 caggcgcgcg tggaccagct cgccgccgaa atcgacgcga tcaagttgcg caaatccgaa
      961 gccgaaggtg cgcgcgacga cgcccagcgc gagctctaca ccgcgcaccg ccgcgtcgcc
     1021 gaactcgccg gccagataca aagccatagc ggccgcatcg aaacggcacg cacgcgcgtg
     1081 gtcaagatcg acgaggaaat cgcggctctg caggcacgca cgcaaaccga cgaagcggcc
     1141 acacgcgaag cgcgcgcccg gctcgaacaa gctgtcgcgc gcatgggcga agaagaacag
     1201 cgtcgccagc acctggatgg cgagcgccgg cgattgctcg aagcgcgcga ggaagcgcgc
     1261 atgaacgcac gcgaagcagg cgaacaggcg caccagcacg cgcttggcat cgaatccaag
     1321 cgtaccggcg tggcggcgct cgaacaggca ctgttgcgca tgagcagcca gttcgcccag
     1381 ctcgaggcgc gcaaggcgga aatcgcgcaa cagctcgaat ccagccaagc gccgctggcc
     1441 ggcatcgagg ccgagcgcca gacctatctc aaccagcgcc tgctcgtcga caagcaactg
     1501 gtcgaggcgc gcaagggcgt ggaagaacgc gacaacgaat tccgcggcta tgaaactgag
     1561 cgccatcgca tcgaacacgt gttgtcgcag caccgcgagg cccacgccga gcggcgcctg
     1621 acgcaccaaa gcctgcagat gcgtgccgag cagttgcagg aagcgatcgc cgcggctggc
     1681 catgaactgc aggccgtgct cgatgcgctg ccggctggtg ccgatccggc cgagtggcag
     1741 cagcagctcg aagccctgga cgccaagatc aagcgcctgg agccggtgaa cctggccgcg
     1801 atttccgagt tcgaggaaca gtcgcagcgc aagacctatc tcgacgccca gctcaccgac
     1861 ctcaacaccg cgctggaaac gctcgaaggc gcgatcaaga agatcgatcg cgagacccgc
     1921 cagcgtttca aggaaacctt cgaccgcgtc aacacgggtt tgcaggaact cttcccgcgc
//
LOCUS       DASRVO010000821         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_336606, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000821 DASRVO010000000
VERSION     DASRVO010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336606"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFF05_10140"
     CDS             <1..600
                     /locus_tag="VFF05_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-ligase"
                     /protein_id="HZW52212.1"
                     /translation="PALDAIVSATAPLDAQLADAAEIRYSTHVIELFGSTETCVIARR
                     RAARDEPWGLYPGVSLHPQPDGTLVDAPYFPAPTLLHDIVELLPDRGFRLCGRNGDLL
                     EIAGKRASLADLNRRLLAIPGVRDGAIFQLDADAVGVRRLAALIVAPGMDVGKILDAL
                     RAGVDPVFLPRPLKIVDALPRNAAGKLPRAALLVALRRA"
     gene            complement(613..1089)
                     /locus_tag="VFF05_10145"
     CDS             complement(613..1089)
                     /locus_tag="VFF05_10145"
                     /inference="COORDINATES: protein motif:HMM:NF022639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HZW52213.1"
                     /translation="MKSLHVLGRVLIASMLSGAGGAAFAQGKPASDPLPPVQAPPSMN
                     DPGVKQAAPAAQAAAAPATKPAEKAQEQSTLPPDVQAAANVAELPVVTVRQNGNETVE
                     EYRKKGKLLFVRVLEKQGPTKFYVDNPSVIPPNLMKQLSGPSGVVQPVYYKLADWK"
     gene            1222..>1921
                     /locus_tag="VFF05_10150"
     CDS             1222..>1921
                     /locus_tag="VFF05_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010369643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HZW52214.1"
                     /translation="MSRPTLVLIDGSGYLYRAFHALPPLSNRMGEPTGALFGVFNMLR
                     ATLKEKPAHVAFVADASGPTFRDAIYAEYKAHRPPMPDDLRTQIEPMLALVEALGFPI
                     LRVPGVEADDVIGTLALQAAAQGIDVVVSTGDKDLAQLVSPHIHLVNTMTNSTLDADG
                     VFAKFGVRPEQIVDYLALVGDSADNIPGVPKCGPKTAAKWLAEYGTLDGIVANADKIG
                     GKIGESLRAALPQLP"
BASE COUNT          311 a          655 c          600 g          355 t
ORIGIN      
        1 cccgcgctcg acgccatcgt cagcgcgacc gcaccactgg atgcgcaact cgcggacgct
       61 gcggaaatcc gctactcgac gcacgtgatc gaattgttcg gttccaccga aacctgcgtc
      121 atcgcccggc gccgcgccgc acgcgacgaa ccgtggggtc tttatcccgg cgtgagcctg
      181 catccgcagc ccgacggcac gttggtcgac gcgccgtatt tcccggcgcc cacgctgttg
      241 cacgacatcg tcgagctgct gcccgataga ggcttccgcc tgtgcggccg caatggcgat
      301 ttgctggaaa ttgccggcaa gcgcgcttcc ctagcagact tgaaccgccg tctgctcgcc
      361 attcccggcg tgcgcgacgg cgcgattttc cagctcgacg cggacgcggt cggcgtgcgc
      421 cgccttgccg cactcatagt cgcaccggga atggacgttg ggaaaattct cgacgcgctg
      481 cgcgcaggcg tagacccggt cttcctgccg cgtccgctca agatcgtcga tgcgctgccg
      541 cgcaatgcgg cgggcaaatt gccgcgcgcg gcgttgcttg tagcgctgcg ccgagcctga
      601 ggtgcgcggc ggttacttcc aatccgcgag tttgtagtac accggctgca ccacgcccga
      661 cggaccggat aactgtttca tcaggtttgg cgggatcacc gatgggttgt cgacgtagaa
      721 tttcgtcggc ccttgtttct cgagtacgcg cacgaacagc agcttgccct tcttgcggta
      781 ttcctcgacg gtttcgttgc cgttctggcg caccgtcacc accggcagtt cggccacgtt
      841 cgcggcggcc tgcacatccg gcggcagcgt ggattgttcc tgggctttct cggcaggttt
      901 cgtcgcgggt gcggcggccg cttgcgctgc tggcgcggcc tgcttgacgc ccggatcgtt
      961 catggacggc ggcgcctgca ctggcggcag cggatctgaa gccggcttgc cttgggcaaa
     1021 agccgcaccg cctgcgccac tcagcatcga agcgatcaac accctgccaa gcacatgtaa
     1081 ggatttcatc tcgcacctcc cgcgttcgtg tcgagcatag catgcacgtg ttgcatttta
     1141 tgcgacgcgc gaagcggccc tttcgttgca aaaatccgcg cactgggcca agctgaaaaa
     1201 cccgccacgg cctgcaccat catgtcgcgc cccaccctcg tcctgatcga cggctccggc
     1261 tatctgtacc gcgccttcca cgcgctgccg ccgttgtcca accgcatggg cgagccgacc
     1321 ggcgcgctgt tcggcgtgtt caacatgctg cgtgcgacgc tgaaggaaaa acccgcgcat
     1381 gtcgccttcg tcgcggacgc gtccgggccg actttccgcg acgcgatcta cgccgaatac
     1441 aaggcgcatc gcccgccgat gccggacgac ctgcgcacac agatcgagcc gatgctggcg
     1501 ctggtcgagg cgttggggtt tcccatcctg cgcgtgccag gcgtcgaggc cgacgacgtg
     1561 atcggcacgc tcgcgctgca ggcggctgcc caaggcatcg acgtcgttgt ctccaccggc
     1621 gacaaggatc tggcgcaact ggtttcgccg cacatccacc tcgtcaacac gatgacgaat
     1681 tcgacgctgg acgcggacgg cgtcttcgcc aagttcggcg tgcgcccgga acagatcgtg
     1741 gattaccttg ccctggtcgg cgacagcgcg gacaacattc cgggcgtgcc caagtgcggc
     1801 ccgaaaaccg cggcgaagtg gctggccgaa tacggcacgc tggatggaat cgtcgcgaac
     1861 gcggacaaga tcggcggcaa gattggcgaa agtctgcgtg ccgcgctgcc gcaactgccc
     1921 a
//
LOCUS       DASRVO010000822         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_51230, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000822 DASRVO010000000
VERSION     DASRVO010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51230"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="VFF05_10155"
     CDS             <1..1563
                     /locus_tag="VFF05_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZW52215.1"
                     /translation="KRVCADNKDSDAIRARMPKPAAKKNGRRIACVGGGPASLTVARD
                     LAPLGYHVTVFEADAKGGGFMRTQVPRFRLPEEVIDEEVGYILDLGVELRGNTRIDSM
                     KDLLAQKFDAVFVGSGAPRGRDLEVPGRREAAANIHIGLDWLASVYFEHIKKIGRRVI
                     VLGGGNTAMDCCRSSRRLGGEDVKVIVRSGFEEMKASPWEKEDAQHEGIPILNYLVPK
                     TFVHENGKLAGMTFEKVRAELDAKGRRKLVPTGEADAFFECDDVLIAIGQENAFPWIE
                     RDIGIEFDAQWDMPKIDKVTMQSTLPNVFFGGDAAFGPKNIIWAVAHGHDAAVSIDKL
                     LSGENIHERPEPGVSLISQKMGIHEWSYDNDISNDTRYAVPWRDKAQTLKSIKTEVEL
                     GFDAKSALAEAERCLNCDVQTVFTKDVCIECDACVDICPMDCITFTDNGEEAELRTHL
                     SAPALNLAQALYVSDTLKTGRVMVKDEDVCLHCGLCAERCPTGAWDMRKFFLEMTHAG
                     PGCHKHAKREAA"
     gene            1560..>2084
                     /locus_tag="VFF05_10160"
     CDS             1560..>2084
                     /locus_tag="VFF05_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZW52216.1"
                     /translation="MSESNKIGLEATNDFVVKFANVNGSGSASANELFAKSILRMGVP
                     VSPRNIFPSNIQGLPTWYEVRVTEQGWLGRRGGIDLMVAMNPQTWDADVAEIAPGGYL
                     FYDNTRPLPASKFRADINVIGMPLTAICNRTYADARQRQLFKNIMYIGALSALLGIDP
                     AEIEKLFGEQYKGKE"
BASE COUNT          428 a          632 c          677 g          347 t
ORIGIN      
        1 aagcgtgtct gcgcggacaa caaggacagc gacgcgatcc gcgcgcgcat gcccaaaccg
       61 gccgcgaaga aaaacggcag gcgcattgcc tgcgtcggtg gcggcccggc ttcgcttacg
      121 gtcgcgcgcg acctcgcgcc gcttggctac cacgtcaccg tgttcgaggc cgacgccaag
      181 ggcggcggtt tcatgcgcac gcaggtgccg cgctttcgcc tgcccgagga agtgatcgac
      241 gaggaagtcg gctacatcct cgatctcggc gtcgaattgc gcggcaatac gcgcatcgat
      301 tcgatgaagg acttgctcgc gcagaaattc gacgcggtct tcgtcggctc cggcgcgccg
      361 cgcggacgcg acctggaagt ccctggtcgc cgtgaggccg ccgcgaacat tcatatcggc
      421 ctggattggc tggcctcggt gtatttcgag cacatcaaga aaatcggccg gcgagtgatc
      481 gtgctcggcg gcggcaacac ggcgatggac tgctgccgtt cgtcgcggcg tctcggcggc
      541 gaggacgtga aggtgatcgt gcgttcggga ttcgaggaaa tgaaggcctc gccgtgggaa
      601 aaagaggacg cgcagcacga gggcattccg atcctcaatt acctcgtgcc gaaaaccttc
      661 gtgcacgaaa acggcaagct cgccggcatg acgttcgaga aagtgcgcgc cgaactcgat
      721 gccaagggcc ggcgcaagct ggttcccacg ggcgaggcgg atgcgttctt cgaatgcgat
      781 gacgtactaa tcgccatcgg ccaggaaaac gcgtttccgt ggatcgagcg cgacatcggc
      841 atcgaattcg atgcgcaatg ggacatgccg aaaatcgaca aggtcacgat gcagtcgacg
      901 ttgcccaacg tgttcttcgg cggcgacgcg gcattcgggc cgaagaacat catctgggcc
      961 gtggcccacg gccacgacgc ggcggtgtcc atcgacaagc tgctcagcgg cgagaacatc
     1021 cacgagcggc ccgagcccgg cgtgagcctc atctcgcaga agatgggcat ccatgagtgg
     1081 agctacgaca acgacatttc caacgacacg cgctatgccg tgccgtggcg cgacaaggcg
     1141 cagacgctga aaagcatcaa gaccgaagtc gaactgggtt tcgatgcgaa gagcgcgctt
     1201 gccgaggcgg agcggtgtct caactgcgac gtgcagacgg tattcaccaa ggacgtgtgc
     1261 atcgagtgcg acgcttgcgt ggacatctgc ccgatggatt gcatcacgtt caccgataac
     1321 ggtgaggaag ctgaattgcg cacgcacctg tccgccccgg cgctgaacct cgcgcaagcg
     1381 ctgtacgtgt ccgacacgct caagacgggc agggtgatgg tcaaggacga ggacgtctgc
     1441 ctgcattgcg gcctgtgcgc cgagcgttgc ccgaccggcg cctgggacat gcgcaagttc
     1501 ttcctcgaga tgacgcacgc gggtccgggc tgccacaaac atgcgaaaag ggaagccgca
     1561 tgagcgagtc gaacaagatc ggactggaag ccaccaacga cttcgtcgtc aagttcgcca
     1621 acgtcaacgg ctcgggttcg gcgtcggcga acgagttgtt cgccaagtcc atccttcgca
     1681 tgggcgtgcc ggtcagcccg cgcaacatct tcccgtcgaa catccagggc ctgccgacct
     1741 ggtacgaggt gcgcgtgacc gagcagggct ggcttggccg gcgcggtggc atcgatctga
     1801 tggtggcgat gaatccgcag acctgggacg cggacgtggc cgaaatcgcg ccgggcggat
     1861 acctgtttta cgacaacacg cggccgctgc cggcatcgaa attccgcgct gacatcaatg
     1921 tgatcggcat gccgctcacc gcgatctgca accgcaccta cgcggatgcg cgccagcgcc
     1981 agttgttcaa gaacatcatg tacatcggcg cgctgtcggc gttgctcggc atcgatcctg
     2041 ccgaaatcga aaaactcttc ggcgaacagt acaagggcaa ggaa
//
LOCUS       DASRVO010000823         1357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_206888, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000823 DASRVO010000000
VERSION     DASRVO010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206888"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFF05_10165"
     CDS             complement(<1..185)
                     /locus_tag="VFF05_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52217.1"
                     /translation="MRSAFLALITMILLGCAGPTTQPAPPTKDALYRELGGAPGITQV
                     VDLFLVRLNADARINTL"
     gene            481..>1357
                     /locus_tag="VFF05_10170"
     CDS             481..>1357
                     /locus_tag="VFF05_10170"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HZW52218.1"
                     /translation="MKTYLVGGAVRDRLLGLAVKDRDHVVVGATPDAMLRAGFKPVGK
                     DFPVFLHPHTREEYALARTERKTARGYHGFAFDTDPSVTLEDDLRRRDLTINAIAEDE
                     NGELIDPYGGAADLRKGLLRHVSPAFAEDPVRILRVARFAARYASRGFVIAKATLELM
                     RTMVRDGEADHLVPERVWAETRKALAERTPSAFLHALRESGALARVMPEVDALYAVPQ
                     RAEFHPEIDTGAHTEMVLDMAARLAPGDALIGWCALTHDLGKALTPVAELPSHRRHEQ
                     RGLAPLRAMAARLKVP"
BASE COUNT          239 a          437 c          469 g          212 t
ORIGIN      
        1 aacagggtgt tgatgcgcgc atcggcgttc aatcgcacca ggaacagatc gacgacttgc
       61 gtgatgcccg gcgcaccgcc caactcgcgg tagagcgcgt ccttcgtcgg tggcgcgggc
      121 tgcgtggtgg ggccggcaca gccgagcagg atcatggtga tcaaagccag aaatgcagat
      181 ctcatggacg gactccgagg cgagcggagc agtctgccga tcatggcatg cagacggcgt
      241 gaaggcgtgg cgatgcagcc cagctcgaaa tcgtcgcgag cgatggcagt tcgcacgcgg
      301 tcggagcgca ggaaccggag tgtacggatt agtacatgag ggtgagtcga gcgccgcttg
      361 gcgcgcattg cgcgccgcga gagcgaacgc ccgcgccgct ggcgcgggca gggagcgccg
      421 ccggcgcgtg aaatgccgag cgcagcagat ttcaagttgg gttgaccgca taatgcgcgc
      481 atgaaaacct atctggtcgg cggcgcagtg cgcgatcgcc tgctcggcct ggccgtcaag
      541 gatcgcgatc atgtcgtcgt cggcgcaacg cccgacgcga tgttgcgcgc gggattcaag
      601 cccgtgggca aggatttccc ggtattcctg catccgcaca cgcgggagga atacgcgctg
      661 gcgcgcaccg aacgcaagac cgcccgcggc taccacggct tcgccttcga caccgatcca
      721 tcggtgacgc tggaagacga tctacgccgc cgcgacttga ccatcaacgc gatcgccgaa
      781 gacgagaacg gcgaactcat cgatccctat ggcggtgcgg cggatttgcg caagggtttg
      841 ctgcgccatg tatcgcctgc gttcgccgag gaccccgtgc gcatcctgcg cgtggcgcgc
      901 ttcgcggcgc gttatgcgtc gcgcggattc gtcatcgcca aggctacgct ggaattgatg
      961 cgcaccatgg tccgcgacgg cgaggccgat catctggtgc ccgagcgcgt ctgggcggaa
     1021 acgcgcaagg cgctggccga acgcacgccc agcgctttcc tgcacgcgct gcgcgagtcc
     1081 ggtgcactcg cgcgcgtcat gcccgaagtc gatgcgctct acgccgtacc gcagcgtgcc
     1141 gaattccacc ccgagatcga caccggcgcg cacacggaaa tggtgctgga catggctgcg
     1201 cgactcgcgc cgggcgatgc gctgatcggc tggtgcgcgc tcacccacga cctgggcaag
     1261 gcgctcacgc ctgtcgctga attgccgtcg catcgccggc acgaacagcg tggcctagcg
     1321 ccgctgcgcg caatggcggc gcgcctgaaa gtgccgg
//
LOCUS       DASRVO010000824         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_51261, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000824 DASRVO010000000
VERSION     DASRVO010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51261"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1940
                     /locus_tag="VFF05_10175"
     CDS             21..1940
                     /locus_tag="VFF05_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HZW52219.1"
                     /translation="MARNDRHTLAACFLGPYAENDGLLEKLVVEFLRDHVYWRRNFHP
                     EDPPAIATAAAQHPDYLAFEARMRRELHQLSAALKKSVPFHSPRYIGHMASDLLLPGL
                     AAQMLTLPYNPNNVSEDAAPVTVDLEVQVGLQLARMLGYPHDPAQPACAFGHLTSGGT
                     LANYQALRLALSVKAFPLALRAAQVPDLALPPDDWSAFNLHGSAVIELLESWQRWLAA
                     QTPAARVRWHAAVESQRIEQRGLVDFFAAHPQLRVPRVLAPVTAHYSWSKGVKLLGLG
                     RDRLLAVPTRGMRLDVDAFDAIIERCAREREPVLLAVAVLGSTEYGTVDPIDGVLAAR
                     ARSRAHGLDFAVHVDAAWGGYLATLFRKEDGSLRTRAEVAAEFARFPTPAVHAALAAL
                     AGTDSATVDPHKLGYLPYGAGAFVCRDHRAMALLAERADYVFHAETPMDYLARYRSLG
                     QFIPEGSKSGAAAAAVYVTHKVLPLHHAEFGRLPRATVIAAEAFYARALSFAEEQAGR
                     LHALVPFAPDSNLVCLALNPHDNRSPARTNAFMRRLHEALRADPRQPLQTKEFFGSTT
                     SLPAELLGADEAARIFGALGLDMDSADEPLVILRHTLMNPYLLDSENGISYIDRYFEF
                     LARISATLIQGMSCA"
     gene            1937..>2519
                     /locus_tag="VFF05_10180"
     CDS             1937..>2519
                     /locus_tag="VFF05_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZW52220.1"
                     /translation="MNAATPARQFPDSRAWVHEAIDRLAREAARSADTHLLKIELPGF
                     AGIDFYFKDEAAHPTGSLKHRLARSLFLYALCNGRLREGQDVVDASSGSTAIAEAWFA
                     RLLGLSFTAVMPACTAPAKIRAVEALGGACDLVDDPGRVHARAAERAAQGACHLDQFG
                     LAERATDWRGNNNIAESILGQLAHEPQPEPAWIV"
BASE COUNT          405 a          918 c          779 g          417 t
ORIGIN      
        1 ggctacccgc agaaccgcct ttggccagaa acgaccggca cacgctggcc gcgtgttttc
       61 tcggacctta cgccgaaaac gacggcctgc tggaaaagct cgtcgtggaa ttcctgcgcg
      121 accatgtcta ctggcggcgc aactttcacc cggaggatcc gccggcgatc gcgaccgcgg
      181 ccgcgcagca tccggattac cttgcattcg aggcacgcat gcgccgtgag ttgcaccagc
      241 tttcggccgc gctgaaaaaa tcggtgccgt tccacagccc gcgttacatc ggtcacatgg
      301 catccgacct gctgctgccc ggtctcgccg cgcaaatgct gacgctgccg tacaacccga
      361 acaacgtcag tgaggacgcc gcgccggtta ccgtcgatct ggaggtgcag gtcggtctgc
      421 agctcgcgcg catgctcggc tacccgcacg acccggcgca accggcctgc gcgtttggcc
      481 atctgacttc cggcggcacg ctggccaatt atcaggctct gcgcctggcc ttgtccgtca
      541 aggcgtttcc actcgccttg cgcgcagcgc aggtgcccga cctcgccctg ccgccggacg
      601 actggagcgc attcaatctg cacggcagcg cggtaatcga gttgctcgaa tcgtggcagc
      661 gctggctcgc tgcgcagacg ccggcggcgc gagtgcgctg gcacgccgcg gtcgaaagcc
      721 agcgtatcga gcaacgcggc ctcgtcgatt ttttcgccgc gcacccgcaa ctgcgcgtgc
      781 cgcgcgtgct cgcaccagtc accgcgcact attcgtggag caagggcgtc aaactgctcg
      841 gcctcggccg cgatcgattg ctcgccgtgc cgacgcgcgg catgcgtctg gacgtcgacg
      901 ccttcgacgc catcatcgag cgctgcgcac gcgaacgcga gccggtattg ctggcggtcg
      961 ccgtgctcgg cagcaccgag tacggcacgg tcgatccgat cgacggcgtg ctcgccgcgc
     1021 gtgcgcgcag ccgcgcgcac ggactcgatt tcgccgtgca cgttgatgct gcctggggcg
     1081 gttatctcgc cacgctgttc cgcaaagaag acggcagcct gcgcacacgc gccgaagtcg
     1141 ccgccgaatt cgcgcgcttc ccgacgccgg ccgtgcacgc cgcgctcgcc gcgctcgccg
     1201 gcaccgattc ggccaccgtc gacccgcaca agctcggcta tctgccgtac ggcgccggcg
     1261 cgttcgtctg ccgcgaccac cgcgcgatgg ccctgctcgc cgagcgcgcc gattacgtgt
     1321 tccacgccga aacaccgatg gactacctcg cgcgttaccg cagtctcggc cagttcatcc
     1381 cggaaggctc caagtccggc gctgccgccg ctgcggttta cgtgacgcac aaggtgttgc
     1441 cgctgcatca cgccgaattc ggtcggttgc cacgtgcgac cgtgattgcg gcagaagcgt
     1501 tctacgcgcg tgcgctgagt tttgccgaag aacaggccgg ccgcctgcac gcgttggtgc
     1561 cgttcgcgcc ggacagcaac ctggtctgcc tcgcgctcaa cccgcacgac aaccgctcgc
     1621 cagcgcgcac caacgctttc atgcgccggc tgcacgaggc gctgcgcgcc gatccgcgcc
     1681 agccgctgca gaccaaggaa ttcttcggct cgacgaccag cctaccggca gaactgctag
     1741 gcgccgacga ggccgcacgc atcttcggcg cgcttggact cgatatggac tcggcggatg
     1801 agccgctggt catcctgcgc cacaccttga tgaatccgta cctgctcgac agcgagaacg
     1861 gcatcagcta catcgaccgt tacttcgaat tcctcgcgcg aatcagcgcg acattgatcc
     1921 agggaatgtc ctgcgcatga acgctgccac gcccgcccgg caattcccgg atagccgggc
     1981 ctgggtacac gaggccatcg acagactcgc ccgggaagcc gcgcgctcgg ccgacacgca
     2041 cctgctcaag atcgaattgc ccggctttgc cggcatcgac ttctatttca aggacgaagc
     2101 cgcgcacccg accggcagcc tcaagcaccg gctcgcgcgt tcgcttttcc tgtacgcgtt
     2161 gtgcaacggc cggctgcgcg aaggccagga tgtcgtcgat gcctcatccg gcagcacggc
     2221 gatcgccgaa gcctggttcg cgcgcctgct cggcctctcc ttcaccgccg tgatgccggc
     2281 ctgcaccgcg ccggcgaaaa tccgcgcggt cgaggcactc ggtggcgcct gcgatctcgt
     2341 tgacgatccg ggacgcgtgc atgcgcgcgc ggccgaacgc gctgcgcagg gtgcctgcca
     2401 cctcgaccag ttcggacttg ccgaacgcgc gaccgactgg cgcggcaaca acaacatcgc
     2461 cgaatcgatc ctcggccagc tcgcgcacga gccgcagccg gaaccggcgt ggatcgtct
//
LOCUS       DASRVO010000825         1180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_361123, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000825 DASRVO010000000
VERSION     DASRVO010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361123"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /locus_tag="VFF05_10185"
     CDS             <1..1138
                     /locus_tag="VFF05_10185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HZW52221.1"
                     /translation="TPADDPAQTLHATVHVHDASFYRQVAGNGSVGAGEAYMDSAWTC
                     DDPVSLMRMLVRNRDLLDAMETGLARIGGWTMRAWHALNRNTRDGSRRNIAAHYDLGN
                     DFFRLFLDENLMYSSAIFARADESLEAASTRKLDRICRKLALNPADRVVEIGTGWGGF
                     ALHAARHYGCHVTTTTISREQYDLARARVREAGLEDRIEVVLHDYRDLDGRFDKLVSI
                     EMIEAIGHQYLGSYFGKVGRLLKPDGMALIQAITIEDHRYAQALRAVDFIKRYIFPGS
                     FIPAVGVMLEVAARASDLKLFHLEDIGPSYALTLAAWRERFHRRIADVRALGYDERFV
                     RMWDFYLAYCEAGFHERSTGDVQLLLVKPGCRRAAFLPDLDAPP"
BASE COUNT          205 a          386 c          365 g          224 t
ORIGIN      
        1 cacgcctgcg gacgatcccg cacagaccct gcacgcgacg gtgcacgtgc acgatgcgag
       61 cttctaccgt caggtcgccg gcaacggcag cgtcggcgcc ggtgaagcgt acatggattc
      121 agcgtggacc tgcgacgacc cggtgtcgtt gatgcgcatg ctggtgcgca accgcgatct
      181 gctcgacgcg atggaaaccg ggctggcgcg cattggcggc tggacaatgc gtgcctggca
      241 cgcgctcaat cgcaatacgc gcgacggcag ccggcgcaat atcgcggcgc actatgacct
      301 gggcaacgat ttcttccgcc tgttcctcga cgaaaacctg atgtactcct cggcgatctt
      361 cgcgcgcgcg gacgagtcgc tggaagccgc ctccacgcgc aagctcgatc gcatctgccg
      421 caagctcgcg ctgaatccgg ccgaccgcgt cgtcgaaatc ggcactggct ggggcggttt
      481 cgccctgcat gcagcgcgcc attacggctg tcatgtgacg acgaccacga tctcgcgcga
      541 acaatacgac cttgcgcgag cgcgcgtgcg cgaagccggg ctggaagatc gcatcgaagt
      601 cgtgctgcac gactatcgcg atctcgatgg ccgtttcgac aaactcgtgt cgatcgagat
      661 gatcgaggcg atcggccacc agtacctggg ttcctatttc ggaaaggtcg ggcgcctgct
      721 caagcccgac ggcatggccc tgatccaggc catcaccatc gaggatcacc gttacgcaca
      781 ggccctgcgc gccgtggatt tcatcaagcg ctacatcttt ccgggcagtt tcattccggc
      841 agttggcgtg atgctcgaag tcgcggcacg cgcgagcgat ctgaaattgt tccacctcga
      901 ggacatcggc ccgagctatg cgctcacctt ggcggcctgg cgcgagcgtt tccatcggcg
      961 catcgccgac gttcgcgcgc tgggctacga cgagcgtttc gtgcgcatgt gggatttcta
     1021 tctcgcctac tgcgaagccg gattccacga acgctccaca ggcgacgtgc agctgctgct
     1081 ggtcaagccc ggctgccggc gcgcggcgtt ccttcccgat ctggacgcgc cgccgtgaat
     1141 ctctggatca acgtcgtttt ttatcaagcc acatggctgg
//
LOCUS       DASRVO010000826         1848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_153343, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000826 DASRVO010000000
VERSION     DASRVO010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153343"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VFF05_10190"
     CDS             complement(<1..155)
                     /locus_tag="VFF05_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HZW52222.1"
                     /translation="MMERVLIIGAGGWGREVLHQMTHDDAAHGVEWYVDGFLDGRTHV
                     LDGYDVG"
     gene            complement(152..904)
                     /locus_tag="VFF05_10195"
     CDS             complement(152..904)
                     /locus_tag="VFF05_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019302783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZW52223.1"
                     /translation="MSNPAQKAFSLEGKTILVTGASSGLGREIALACARRGARIVITG
                     RDASRLRETHEQLAGTGHVRVTADLTSAEDRERLVQACGNIDGLVLCFGGQMLSPIRQ
                     LKEELMTAMYRTHFLAPVMLTQRLLQTNSINKQGSIVFMLSTAAHIGTRGVGPYSAMK
                     SGLLGIIRCLAIELAPRKVRVNGISPSVVPTPQLWGEDNEQNEPLNKERARHPLGLGT
                     PQDVGNGAVYLLADASRWVTGTTLVMDGGAVL"
     gene            complement(901..1662)
                     /locus_tag="VFF05_10200"
     CDS             complement(901..1662)
                     /locus_tag="VFF05_10200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010373659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZW52224.1"
                     /translation="MTAADPFSLAGKRILVTGASSGIGRQIAVTCAQMGAQLVVSGRN
                     TERLAATLAELEGDGHAPIAADLSRQEDIDRLAADAGTVDGLVHAAGISKLAPFRLIS
                     RAQLDEQFSSNVYAPMLLTKGLLAKKRIANGGSILFISALASHVGPLATGVYSASKAA
                     LLGAMRTLALEVVKQGIRANCIAPGYVRTPMLEGLNQGGGSIGEHAKLTPLGLGEPED
                     VAHAAVFYLSDASRWVTRNYFIVDGGLTVPMDIYA"
     gene            complement(1665..>1848)
                     /locus_tag="VFF05_10205"
     CDS             complement(1665..>1848)
                     /locus_tag="VFF05_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HZW52225.1"
                     /translation="LRDFGNTSGASLPVTMTARLHDKLAQGRHRLLLCGFGIGLSWGT
                     AIVDIDSGVFPALIEA"
BASE COUNT          346 a          587 c          588 g          327 t
ORIGIN      
        1 atgccgacat cgtagccgtc gaggacatgc gtgcggccat ccagaaaacc gtcgacgtac
       61 cactccacgc catgcgcggc atcgtcgtgc gtcatctggt gcagcacttc cctgccccag
      121 ccgccggcac cgatgatgag aacgcgctcc atcacagcac cgccccgccg tccatgacca
      181 gcgtggtgcc ggtcacccag cggctggcat cggccaacag ataaaccgcg ccgttgccca
      241 cgtcctgcgg ggtgccgagc cccagcgggt ggcgcgcgcg ctccttgttc aggggttcgt
      301 tttgttcgtt gtcttccccc caaagctgcg gcgttggaac aacggatggc gaaatcccgt
      361 tgacgcgcac tttgcgtggg gcgagttcga tggccaggca acgaatgatg ccgagcaaac
      421 cggatttcat cgccgagtac ggtcctacac cacgggtgcc gatatgcgca gcggtcgaca
      481 acatgaaaac gatagaaccc tgcttgttga tcgaatttgt ctgcaggaga cgctgagtca
      541 acatcaccgg agcgagaaaa tgagtacggt acatagcggt catcagttct tccttgagct
      601 ggcggattgg cgaaagcatt tgcccgccga agcacagcac caagccatcg atgttgccgc
      661 aggcctgcac cagacgctcg cggtcctcgg cggaagtcag gtccgccgtc acccgcacat
      721 gccctgtgcc ggcgagctgc tcgtgcgttt cccgcaggcg cgacgcgtcg cggccggtga
      781 tgacgatccg cgcgccgcgt cgtgcgcagg ccaaggcgat ctcgcggccc aggccggacg
      841 aggcgccggt cacgaggatg gttttgcctt ccagcgaaaa cgccttttgc gccgggttgc
      901 tcatgcgtag atatccatcg gcacggtcaa gccgccatcg acgatgaaat aattgcgcgt
      961 tacccagcgg ctggcatcag agaggtagaa cacggcggca tgggcgacgt cttccggttc
     1021 acccaacccg agcggcgtca atttggcatg ctcgccgatg ctgccgccgc cctggttcaa
     1081 gccttccagc atgggagtgc gcacgtaacc cggtgcgatg cagttcgcgc gaatgccctg
     1141 cttgacgacc tccagtgcca gcgtgcgcat ggcgccgaga agcgccgcct tgctggccga
     1201 atatacgccg gttgccagcg ggccaacatg cgacgccaat gcggaaatga acaggatgga
     1261 accgccgttt gcgatgcgct ttttcgccag caggcctttg gtcaaaagca tcggcgcata
     1321 gacattgctg gaaaattgtt cgtccagttg tgcccgcgaa ataaggcgaa atggcgcgag
     1381 tttggaaatg cccgccgcgt gtaccaggcc gtcgaccgtg ccggcatcgg cggcgaggcg
     1441 gtcgatgtct tcctgccgcg acagatcggc ggcgattggc gcatggccgt cgccttccag
     1501 ttcggccagc gtggccgcga ggcgttcggt gttgcgtccg ctgacgacaa gctgtgcgcc
     1561 catctgtgcg caagtgacgg cgatttgccg gccgatgccg gaagacgcgc cggtgacgag
     1621 aatgcgcttg cccgccagcg aaaacgggtc cgccgcagtc acgcttacgc ctcgatcaag
     1681 gccgggaaaa cgccggaatc gatgtcgacg atggccgtgc cccaggacaa gccgatgccg
     1741 aagccgcaca acagcaggcg atgcctgcct tgcgcgagtt tgtcgtgcag gcgtgccgtc
     1801 atcgtcaccg gcagggatgc accgctggtg ttgccgaagt cgcgcagg
//
LOCUS       DASRVO010000827         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_129054, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000827 DASRVO010000000
VERSION     DASRVO010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129054"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VFF05_10210"
     CDS             <1..316
                     /locus_tag="VFF05_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HZW52226.1"
                     /translation="LPYPHGYVEKYANDVYLRKATVDYLGSARYDDVLAVCCRVGRLG
                     RSSMTFHFEIFRDDPDPAPAPLVTAELVYVNADPSSMTPAPLPQELRERIARYERVAP
                     QT"
     gene            313..771
                     /locus_tag="VFF05_10215"
     CDS             313..771
                     /locus_tag="VFF05_10215"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HZW52227.1"
                     /translation="MSAGKTLYWEDFRVGETVAMGNHTFAEEEMIDFARKFDPQPFHI
                     DPVAAKNSYFGELIASGWHTCAVAMRLLCENYINRSASLGSPGIENVRWLKPVRAGDT
                     ISYRRVVLESRPSNSRRDMGLVKSRTEAVNQKGEMVMTFEGWGMFGRRPS"
     gene            complement(796..1977)
                     /gene="clsB"
                     /locus_tag="VFF05_10220"
     CDS             complement(796..1977)
                     /gene="clsB"
                     /locus_tag="VFF05_10220"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HZW52228.1"
                     /translation="MRFVPGNRLQLLRNGEEYFPALVAAIDAAATEVFLETYIFADDE
                     TGSLVTDALARAAARGAAVHVLLDGFGAREFAPRFRAMLRQSGAEVLVFRPQISPWTL
                     RRGRLRRMHRKLAYIDGRVAFVGGINVIDDYDTLARMPPRFDYAVRIEGPLTEEVRAA
                     AARLWSQVSWATFRRGRQGFAVLARKAMRAAPDTPRAMPGGQRVALVVRDNVRHRRDI
                     EEGYLQFIGEAREEIVLANAYFFPGRRFRRALADAAARGVRVVLLLQGKVEYTLLHYA
                     SRALYGAMLDSGIEIHEYTRSFLHAKVAVFDHRISSVGSSNIDPFSLMLAREANVFID
                     DPAFAAQLRSSLEEAMRTSSRAMPPRLWRRQPLWQRVRIWVAYGITRLLISFAGLERH
                     H"
BASE COUNT          375 a          702 c          685 g          320 t
ORIGIN      
        1 cctaccctac ccgcacggct atgtcgaaaa gtacgccaac gacgtctacc tgaggaaggc
       61 gacggtcgat tacctcggct ccgcgcgcta cgacgatgtg ctcgcggtgt gctgccgggt
      121 aggccggctg ggccgcagca gcatgacttt ccacttcgag atcttccgcg acgacccgga
      181 tccggcgccc gcgcccctcg tgaccgccga actggtctac gtcaacgccg acccgtcgag
      241 catgacgccc gcgccgctgc cgcaggaact tcgcgagcgc atcgctcgct acgaacgcgt
      301 tgcgccgcag acatgagcgc cggcaagacc ctgtactggg aggacttcag ggtcggcgag
      361 accgtagcca tgggaaacca cacattcgcc gaggaagaga tgatcgactt cgcgcgcaag
      421 ttcgatcccc agccgttcca catcgacccg gttgcggcga agaactcgta cttcggcgag
      481 ttgatcgcga gcggctggca tacgtgcgcg gtggcgatgc gtctgctttg cgaaaactac
      541 atcaatcgca gcgcgagcct gggctcgccc gggatcgaga acgtgcgctg gctgaagccg
      601 gtccgcgcgg gcgatacgat ttcctaccgc cgcgtggtgc tcgaatcgcg cccgtcgaac
      661 agccgtcgcg acatgggact ggtcaagtcg cgcaccgagg ccgtgaacca gaaaggcgag
      721 atggtcatga ccttcgaagg ttggggcatg ttcggccgac gtccaagctg aggcatcggg
      781 cgcacggcag agcgtctagt ggtggcgctc cagacccgca aagctgatca gcaacctggt
      841 tatcccgtac gctacccaga tgcgcacgcg ctgccacagg ggttggcgcc tccacagccg
      901 aggcggcatc gcacgcgagc tcgtacgcat cgcctcctcc aggctggagc gcagttgcgc
      961 ggcgaaggcc gggtcgtcta tgaacacatt cgcctcgcgc gcaagcatca gactgaacgg
     1021 gtcgatgttg gaagagccca cgcttgaaat gcggtggtcg aacactgcga ccttggcgtg
     1081 caggaagctg cgcgtgtact cgtgaatttc gatgccggaa tcgagcatcg ccccgtaaag
     1141 cgcacgggaa gcgtagtgaa gcagcgtgta ctcgaccttt ccctgcagta gcagcacgac
     1201 gcgtacgccg cgtgcggcag cgtctgccag cgcgcgccgg aaacgccgtc cgggaaagaa
     1261 gtacgcgttc gcgagcacga tttcttcgcg cgcctcgccg atgaactgga ggtatccctc
     1321 ttcgatgtcc cgccggtgtc gcacgttatc gcgcaccacg agcgccacgc gctgcccccc
     1381 gggcatcgcc cgcggcgtat cgggcgcggc gcgcatcgcc tttctcgcca gcacggcgaa
     1441 accctgcctc ccccgccgga acgtcgccca ggacacctgc gaccagagac gcgcggctgc
     1501 cgcgcgcacc tcctcggtga gcggcccctc gatccgcact gcgtagtcga agcggggcgg
     1561 catgcgggcc agcgtatcgt agtcgtcgat gacgttgatg ccgcccacga acgccacgcg
     1621 gccgtcgatg tacgccagct tgcggtgcat ccgccgcagg cgcccgcggc gcagtgtcca
     1681 agggctgatc tgcggccgga atacgagcac ttcggcgccg gattgccgca gcatggcgcg
     1741 aaagcgcggc gcgaactcgc gcgcgccgaa gccgtcgagc agaacgtgta ccgccgcgcc
     1801 gcgggcggcc gcgcgcgcaa gcgcgtccgt caccaggctg ccggtctcgt cgtcggcgaa
     1861 gatataggtt tcgaggaaca cttccgtggc ggccgcatcg atggccgcga ccagcgccgg
     1921 aaagtattcc tcgccgttgc gcagcagctg caggcggttg cctggaacga accgcacgca
     1981 ctaggcccgc gtcagttcgg cgctgagcgc gagatggtcg gaaagcaagg accagggcac
     2041 accgcggtga acttgcgcgt cgagcacgtc gaagccgcgg ac
//
LOCUS       DASRVO010000828         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_361189, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000828 DASRVO010000000
VERSION     DASRVO010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361189"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFF05_10225"
     CDS             <1..189
                     /locus_tag="VFF05_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52229.1"
                     /translation="FVLSASMLGWAAVWVLGTHASNLFGNVHGTLHKVLVVGIAVIVG
                     IANHFHKQRERAKKRDGR"
     gene            179..376
                     /locus_tag="VFF05_10230"
     CDS             179..376
                     /locus_tag="VFF05_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HZW52230.1"
                     /translation="MAADKKAYPLRISADVLDAVQRWADDELRSVNAQIEYVLREALR
                     KAGRLKAQQPSAPNPVRGKAS"
     gene            373..1707
                     /locus_tag="VFF05_10235"
     CDS             373..1707
                     /locus_tag="VFF05_10235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZW52231.1"
                     /translation="MKSAAIFASALLAGAGTSAHAGDTAQNVCMQRAQDILSALHKGD
                     YATATAHFDARMLAALNAGKLQSVWHDVLPAQVGAFDHAATTTLRETTKTELAETPLK
                     FARAWLTMRVACNADGTVGGLFFAPGSAPASEAAAVPSNERPLAVSSPLGPLPGTLDM
                     PQGDGPFPAVLLVAGSGPNDRDETIGPNKPFADIAHGLAEQGIATFRYDKRTRVYGAQ
                     IAGRPITIDDEVTDDAIAALKLLAQQPNINPHRIFVLGHSLGAFAAPRIAAHDSRVAG
                     TILLAAPATFDLDTVVRQTRYLARIEHATPQQTQAALAPIEAARDAIARADPKHPPTG
                     EFFHAPASYWLSLRGYDPIAVSKNLKQPILVLQGTGDYQVTPTADFAKWQATFARDPR
                     VTLKEYPGLSHLFMAAGDPPSPADYAKAGHVDARVIADIAAWIAAQPRSEVH"
     gene            1712..2269
                     /locus_tag="VFF05_10240"
     CDS             1712..2269
                     /locus_tag="VFF05_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZW52232.1"
                     /translation="MSDSSKPSPISAARCRELIESAFALGVPRDYGRVRKLRRVREPT
                     HLASIGLDTQGREQFLALRAARAWARMHDAALDDGAELQVVSAFRSIEYQLGIIRRKL
                     ERGQSMQQILRVSAAPGYSEHHSGRALDVTAPGFAALEEEFENSPAFAWLKKHARRFG
                     FHLSYPRKNRHGIAYEPWHWCWKSR"
     gene            complement(2429..>3422)
                     /locus_tag="VFF05_10245"
     CDS             complement(2429..>3422)
                     /locus_tag="VFF05_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HZW52233.1"
                     /translation="TADIQAVQLNNQPVDAQLLLPYLLAAAHNAQADGAWAPLQQLAS
                     DPAVSEAIARLSAWDLSTPTGLTQGFDAGRAPGSAPTSPQLDNSVAATEFFMWRSNAI
                     RNTIDGTLTAYGLGDYLPAGDSAYTGLKFLLDAFPVLGGHGASGINFFNVPNAPDAAS
                     ARDFILLTSLKQGLTQLAGNDFAAAFGNSTDQSTYHWGALHRFVLEHPLGGPFNIPGP
                     NPYGFTALSPDLPGLARDSSYSTVNPGGHALRVSGVNDFMFSGATAPGRRFVGQMSTP
                     INAVEVIPGGESAVLGDPNYASQLLLWLTGNYHPLVVDPNAATQGATATVNFVP"
BASE COUNT          668 a         1141 c         1064 g          549 t
ORIGIN      
        1 ttcgtattga gcgcatccat gctcggttgg gctgcggtat gggttctcgg cacacacgcc
       61 tcgaatctgt tcggcaatgt ccatggaaca ctgcacaagg tcctggtcgt cggcatcgcg
      121 gtgatcgtcg gcattgccaa ccacttccac aagcagcgcg agcgcgcaaa aaaacgcgat
      181 ggccgctgac aagaaagcct atccgctgcg catctccgcc gacgtgctcg acgccgtgca
      241 acgctgggcc gacgacgagc tgcgcagcgt caacgcacag atcgaatacg tgctgcgcga
      301 ggcgctgcgc aaggccgggc ggctgaaggc gcagcagcca tcggctccaa atcccgtcag
      361 aggcaaggca tcatgaaatc cgcggcgata tttgcatcgg cgttgctggc cggtgccggc
      421 acgagtgcgc atgccggcga tacagcgcag aacgtctgca tgcagcgcgc gcaggacatc
      481 ctttccgccc tgcacaaggg cgattacgcg acagccaccg cgcacttcga tgcgcgcatg
      541 cttgccgcgc tcaatgccgg caaactccag agcgtatggc acgatgtgct gccggcacaa
      601 gtcggtgcgt tcgatcatgc ggcaaccact acactgcgcg aaaccaccaa aaccgaactg
      661 gccgaaacgc cgctgaaatt cgcccgtgcg tggctgacga tgcgcgtggc ctgcaacgcc
      721 gacggcacgg ttggcggctt gttcttcgca cccggaagcg cgccggcgag cgaagctgcc
      781 gcagtgccat cgaacgaacg gccgctggcg gtttcgtcgc cgctggggcc gctgcccggc
      841 acattggaca tgccgcaagg cgacggcccg tttccagccg tgttgctggt ggccggctcc
      901 gggccgaacg accgcgacga aaccatcggc cccaacaagc cgttcgccga catcgcgcat
      961 ggcctggccg aacaaggcat tgccacgttc cgttacgaca aacgcacccg ggtttatggc
     1021 gcgcagatcg ccggcaggcc catcaccatc gacgacgagg tcacggacga cgccatcgcc
     1081 gcactgaaac tgctcgcgca acaaccgaac atcaatccgc accgcatatt cgtgctggga
     1141 cacagccttg gcgccttcgc cgcgccgcgc attgctgcgc acgattcccg tgtcgctggc
     1201 acgatcctgc ttgcggcgcc ggcgacgttc gatctggaca ccgtggtgcg acaaacccgc
     1261 tatctagcca ggatcgaaca cgccacgccc caacaaacgc aagcagcgct ggcaccgatc
     1321 gaagcggcgc gcgacgccat cgccagggcc gatccgaagc atccgccgac gggcgaattc
     1381 ttccacgctc cagcgagcta ttggctgagc ctgcgcggtt acgatccgat cgccgtatcg
     1441 aagaacctca aacaaccgat cctagtcctg caaggcaccg gcgactatca ggttacgccc
     1501 acggccgact tcgccaaatg gcaagcgacg ttcgcacgcg acccgcgcgt cacgctgaag
     1561 gaatatcccg gtctgagtca cctgttcatg gcggccggcg atccgccttc gccggccgac
     1621 tatgcaaagg ccggacacgt cgatgcacgg gtgatcgccg atatcgcggc gtggatcgcg
     1681 gcgcagccgc gcagcgaagt acactgacac ggtgagcgat tcatccaaac cctcccccat
     1741 ttccgctgcg cgttgccgcg aattgatcga gagcgccttc gccctcggcg tgccgcgcga
     1801 ctacggtcgg gtgcgcaagc tgcgacgtgt gcgcgaacca acgcacctgg catccatcgg
     1861 actggacact cagggccgcg aacaattcct cgccctgcgc gccgcgcgag catgggctcg
     1921 catgcacgat gccgcattgg acgacggcgc ggagttgcag gtcgtctcgg cgttccgctc
     1981 gatcgaatac cagctcggca tcatccgccg caaactcgaa cgcgggcaat cgatgcagca
     2041 gatcctgcgc gtcagcgcgg cgcccggata cagcgaacat cactccggac gtgcgctgga
     2101 cgtcactgca cccggattcg ccgcgctgga agaagaattc gagaactcac cggctttcgc
     2161 atggctgaag aaacacgcgc gacgtttcgg ctttcatctg agctatccgc gcaaaaaccg
     2221 ccatggcatc gcctacgagc catggcactg gtgctggaaa tcgcgttaac gcgatgcgtt
     2281 gaagcgcatc gcgtgcgatt tccagcggcg aggcaggacg ccgagcggag gtgaactcca
     2341 aggatggatt cttgccgaag cagtccaccc tcacccgccg cgcgtcggcc tctcccgcca
     2401 gcgggagagg cgtcaactgc aagcaacctc acggcacgaa attcaccgtc gccgtcgcgc
     2461 cctgcgtggc cgcgttcgga tcgaccacaa gcgggtggta attgccggtc agccacaaca
     2521 gcaactggct cgcataattc ggatcgccga gcacggccga ttcgccgccg ggaatcactt
     2581 ccaccgcgtt gatcggcgta ctcatctggc cgacgaaacg gcgacccggc gcggtcgcac
     2641 cgctgaacat gaagtcgttg acgccactta cgcgcaacgc atggccaccg ggattgaccg
     2701 tggagtacga ggagtcgcgc gccaatcccg gcaggtctgg cgacagagcg gtgaagccgt
     2761 agggattggg tcccgggatg ttgaacggcc cgccgagcgg atgctcaagc acgaagcggt
     2821 gcagtgcgcc ccagtgatag gtcgattggt cggtggaatt gccgaaggcg gcggcgaagt
     2881 cgttgccggc caactgtgtc agaccctgtt tcaggctcgt gagcaggatg aaatcacgcg
     2941 cggatgccgc gtcgggcgca tttggcacgt tgaagaaatt gattccggac gcgccatgtc
     3001 cgcccagaac cgggaaggca tcgagcagga acttcagccc cgtgtaggca ctgtcgccgg
     3061 ctggcagata atcgccgagg ccataagcgg tgagcgttcc gtcgatcgtg ttcctgatcg
     3121 cgttgctgcg ccacatgaag aactccgtcg cggcgacgga attgtcgagc tgtggcgacg
     3181 tcggcgcaga acccggtgcg cggcccgcat cgaagccctg cgtcaatccc gttggcgtgg
     3241 acaaatccca cgcggacagg cgcgcgatcg cttcgctcac cgccggatcg ctggccaact
     3301 gctgcagcgg cgcccacgcg ccatccgcct gcgcgttgtg cgcggccgcg agcaggtaag
     3361 gcagcaacaa ctgcgcatcc accggctggt tgttgagctg cacggcctgg atgtcggccg
     3421 tg
//
LOCUS       DASRVO010000829         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_24686, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000829 DASRVO010000000
VERSION     DASRVO010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24686"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFF05_10250"
     CDS             <1..437
                     /locus_tag="VFF05_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019185100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HZW52234.1"
                     /translation="GPRLAVHQANAAALAELLIENDAVEETYFPGLSRHPGHLLARRQ
                     QRGFGAMLSFDLAGGEAAAAAFVDGLECFSLAESLGGVESLVAHPATMTHASMDADAR
                     YRAGIGDGLLRLSVGIEGLEDLRADIERGLQRATLAERACAA"
     gene            473..1723
                     /locus_tag="VFF05_10255"
     CDS             473..1723
                     /locus_tag="VFF05_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZW52235.1"
                     /translation="MRWALIPLCVGLSTGAFAQTPPAGGYVVGDSDFADALSPQARAQ
                     ADRRAAQNLERLRRSGALPSALAQTTVNDLQWPLRPVPGFDQFDYHGTKYFVDHDPRY
                     PGFVQDYTCGTRTYDLASGYNHAGTDYYLWPFAWLMMDQGVVQVVAAAPGTIIEKDDG
                     NFDRQCAFDGSSGANMVYVRQDDGLTAIYMHLRNGSLTSLPVGARVAAGDYLGLVGSS
                     GQSTGPHLHFELRDANGAVVDPRHGQCNAGPDRWAVFQPYEDPHIDTLSTHSAEPVQA
                     ACGYANGANVDDTPSYKNAFAPGDTLWLLASWRDQRNGEVTNFSVLRPDGSVFAQWDF
                     DLASQNLPSPFYSGTAFNWQYVLPEDAPFGAWQFKAVFAGQTYMRTFEVSTFDRTAAG
                     NARKQVGENATRCRPGVVDSACSP"
     gene            complement(1730..2335)
                     /locus_tag="VFF05_10260"
     CDS             complement(1730..2335)
                     /locus_tag="VFF05_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52236.1"
                     /translation="MNRRTLLLVLAALVLVAVWQRSHRAIHHGPGVLVAEIPRQERID
                     RDAFVLRRGDYSVRPLATFSLSARVLSRADYSWDTGAALAPVDLALGWERMSNSAVLD
                     KISISQSGRFYFWRVREFPIPEREIIESSANMHLIPADAAIEREIKRTRDGDIVEFDG
                     YLVKVDGPNGYRWVSSLTRSDSGAGACELVWVEHFAIVPRH"
     gene            complement(2344..>2630)
                     /locus_tag="VFF05_10265"
     CDS             complement(2344..>2630)
                     /locus_tag="VFF05_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HZW52237.1"
                     /translation="VRARKLVELRDIEHRIALHVGTLPPVAAIADEDMERSNAEKTSA
                     VHFLRFELSPAHVAAFKRGDAVDLAVDHSAYQGKTTLDAPTHAALAADID"
BASE COUNT          442 a          846 c          895 g          447 t
ORIGIN      
        1 tcggaccgcg cctggccgtg catcaggcca atgcggccgc gctggcggag ctgctgatcg
       61 agaacgatgc cgtggaagaa acatactttc cgggcctgag ccgtcatccg ggccatctgc
      121 tggcgcgccg ccagcagcgt ggattcggcg cgatgctgag cttcgatctc gctggtggcg
      181 aggccgccgc cgcggccttc gtcgacggcc tggaatgctt cagcctggcc gaatccctcg
      241 gcggcgtgga aagcctcgtc gcgcatccgg cgacgatgac gcacgcgagc atggatgccg
      301 atgcgcgtta ccgcgccggc atcggggacg gactgctgcg gctgtcggtc ggtatcgagg
      361 ggctggagga cctgcgtgcc gacatcgagc gcggcttgca gcgcgccacg cttgccgagc
      421 gcgcctgcgc ggcgtagatt ttttcccgtt tgcagcgttc gggcgtacgc cgatgagatg
      481 ggcgttgatt ccgttgtgcg tgggtttgag cacgggcgcg ttcgcacaga cgccgccggc
      541 gggtggctac gtggtgggcg acagcgattt cgcggacgcg ctgtcgccac aggcgcgcgc
      601 gcaagcggat cgtcgcgccg cgcagaatct cgagcgcctg cgccgcagcg gagcattgcc
      661 atcggcgctc gcgcagacga cggtcaacga cctgcaatgg ccattgcggc cggtgccggg
      721 cttcgatcag ttcgactatc acggcaccaa gtatttcgtc gaccacgatc cgcgctatcc
      781 cggattcgtg caggattaca cctgcggcac gcgtacctat gatctggcga gcggttacaa
      841 ccacgccggc accgactact acctgtggcc gttcgcgtgg ctgatgatgg atcagggcgt
      901 cgtgcaggtc gtcgccgcgg cgccgggcac gatcatcgaa aaggacgacg gcaacttcga
      961 ccgccaatgc gcgttcgacg gcagcagcgg cgcgaacatg gtctacgtgc gccaggacga
     1021 tggcctcacc gcgatctaca tgcacctgcg caacggttcg ctcaccagcc taccggtcgg
     1081 cgcgcgcgtg gcggcgggcg attacctcgg cctggtcggc agttccggcc agtccaccgg
     1141 accgcacctg catttcgaac tgcgcgatgc gaacggcgcc gtggtcgatc ctcggcatgg
     1201 ccagtgcaat gccggaccgg accggtgggc ggtgttccag ccctacgagg atccgcacat
     1261 cgacacgctg agcacgcatt cggccgaacc cgtgcaggcg gcctgcgggt acgcgaacgg
     1321 tgcgaatgtc gacgacacgc cgagttacaa gaacgccttc gcgccgggcg acacgctgtg
     1381 gctgctcgcc tcgtggcgcg accagcgcaa cggcgaagtc acgaacttca gcgtgctgcg
     1441 tccggatggc agcgtgttcg cacaatggga tttcgatctg gccagccaga acctgccgtc
     1501 gccgttctat tccggcaccg cgttcaactg gcaatatgta ttgcccgagg atgcgccgtt
     1561 cggcgcgtgg caattcaagg ccgtgttcgc aggccagact tacatgcgca cattcgaggt
     1621 ctccaccttc gaccgcaccg ccgcggggaa tgcgcgcaag caggtcgggg agaatgccac
     1681 acgctgccgt ccgggcgtcg tggattccgc ttgttcgccg tagccgcgtt caatgtcgcg
     1741 gcacgatcgc gaaatgctcg acccagacca gttcacaggc gccggcgccc gagtcgctgc
     1801 gcgtgagcga gctgacccag cggtagccgt tcggaccgtc cactttgaca aggtagccgt
     1861 cgaactcgac gatgtcgccg tcgcgcgtgc gcttgatttc gcgttcgatg gccgcatcag
     1921 cggggatcag gtgcatgttc gcgctggatt cgatgatctc gcgttccggg atgggaaatt
     1981 cgcgcacccg ccagaagtaa aagcgtccgg attgggaaat ggatatcttg tcgagcacgg
     2041 cgctgttgga catgcgctcc cagccgagcg cgagatcgac cggcgccagc gccgcccccg
     2101 tgtcccagga ataatcggcg cgcgaaagca cgcgcgccga cagcgagaac gtggcgagcg
     2161 gcctgaccga ataatcgccc ctgcgcagca cgaatgcgtc gcggtcgatg cgttcctgcc
     2221 gtgggatttc ggcaacgagc acgccggggc cgtggtgaat cgcgcgatgc gaacgctgcc
     2281 aaacggccac gagcacgagc gcggccagaa cgagcagaag cgtacgccga ttcaatccgg
     2341 cgctcagtcg atatcggcgg ccagcgccgc gtgcgtgggc gcgtccagcg tcgtcttgcc
     2401 ctggtaggcg gagtgatcga cggcgagatc gacggcgtcg ccacgcttga atgccgcgac
     2461 gtgcgcgggc gacaactcga agcgcaggaa atgcacggct gaagtctttt cggcgttgct
     2521 gcgttccatg tcctcgtcgg cgatcgcggc aacaggcggc agagtgccga cgtgcagcgc
     2581 gatgcgatgt tcgatgtcgc gcagttcgac cagcttgcgc gcgcgcactt
//
LOCUS       DASRVO010000830         1459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_76676, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000830 DASRVO010000000
VERSION     DASRVO010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76676"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..>1459)
                     /locus_tag="VFF05_10270"
     CDS             complement(82..>1459)
                     /locus_tag="VFF05_10270"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HZW52238.1"
                     /translation="LDGDKVALDVAQSGIVEKYNYRSDLIVGTLQNVPAVEVKDAELV
                     FAGYGIVAPEYHWNDYAGLDVKGKIVVVLVNDPGHVDPALFKGKNMTYYGRWTYKYEE
                     AARQGAAGCLIVHETGAAGYPWEVVVNSWSGPQFSLVPDAKSPPRLAVAGWLTGDAAQ
                     RLFASAGADLAALEKAAAKPGFTPVPLKATASIAFHNQIRSMQTDNVLALMPGTTRKD
                     EAIVYSAHWDHFGHDPALKGDQIYNGAVDNGTGLSALLEIADAFAHQNPPPQRSILFL
                     VPTMEEAGLLGSQYYTLHPTFPMTKTVVDINMDELIPFGRTRNMAIIGYGQSQMDQYL
                     ADALKPEGRTISGDEEPEKGFFYRSDQLNFARLGVPVLYARGGFDKRDGGEAAGRAAY
                     DDFTAHRYHKPADNYDPSWDLSGVIEDVQALYDVGKRLADESTFPQWLPGSQFGAVRE
                     ASLKAETH"
BASE COUNT          240 a          455 c          488 g          276 t
ORIGIN      
        1 tgcgccagtg cgatcagcgc ggcgacgacg gcaccggcga acagcagcgg gacgatggcg
       61 gcgaagtcga gcatgcgggc ctcagtgggt ttccgccttc aatgatgcct cgcgcacagc
      121 accgaactgg ctgcccggca accattgcgg aaacgtggat tcgtccgcga ggcgcttgcc
      181 gacgtcgtac agcgcttgca cgtcttcgat cacgccggac aaatcccagc tcggatcgta
      241 gttgtcggcc ggcttgtgat agcggtgcgc ggtgaaatca tcgtaggccg cgcgtccggc
      301 cgcctcgccg ccgtcgcgct tgtcgaaacc gccgcgtgcg tacagcaccg gcacgccgag
      361 gcgcgcaaaa ttgagctggt cggagcggta gaagaagccc ttctccggtt cctcgtcgcc
      421 actgatcgtg cggccttcgg gtttcagcgc gtcggcgaga tactgatcca tctgcgattg
      481 gccataaccg attattgcca tgttgcgcgt gcggccgaac ggaatcagct cgtccatgtt
      541 gatgtcgacg actgtcttgg tcatcggaaa tgtcgggtgc agggtgtagt actgcgaacc
      601 gagcagaccg gcttcctcca tcgtcggcac gaggaacagg atcgagcgct gcggcggcgg
      661 attctgatgc gcgaacgcgt cggcgatttc gagcagggcg gaaagcccgg tgccgttgtc
      721 gaccgcaccg ttgtagatct gatcgccctt gagcgcggga tcgtggccga aatgatccca
      781 gtgcgcggag tagacgatgg cctcgtcctt gcgcgtggtg ccgggcatca gcgcgagcac
      841 gttgtcggtc tgcatgctgc ggatctggtt gtggaaggcg atcgacgccg tggccttgag
      901 cggaaccggc gtgaagcccg gcttcgcggc ggctttctcc agtgcggcga gatcggcgcc
      961 ggcgctggcg aacaggcgct gcgcggcgtc gccggtgagc cagccggcca cggccagacg
     1021 cggcggcgat ttggcatccg gcaccagact gaactgcggg ccgctccagc tgttgaccac
     1081 cacttcccac ggataaccgg cggcgccggt ttcgtgcacg atcaggcaac cggccgcgcc
     1141 ctggcgcgcg gcctcttcgt atttgtaggt ccagcgcccg tagtaggtca tgttcttgcc
     1201 cttgaacagg gccgggtcca catgaccggg atcgttgacc agcacgacca cgatcttgcc
     1261 cttgacgtcg aggccggcgt aatcgttcca gtgatattcc ggcgcgacga tgccgtagcc
     1321 ggcgaagacg agttcggcgt ccttgacttc cacggcgggg acgttctgca acgtgccgac
     1381 gatgaggtcg ctgcgatagt tatacttctc cactatgccg ctttgcgcca cgtccagcgc
     1441 aaccttgtcg ccgtcgagc
//
LOCUS       DASRVO010000831         1301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_153520, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000831 DASRVO010000000
VERSION     DASRVO010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153520"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VFF05_10275"
     CDS             <1..631
                     /locus_tag="VFF05_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZW52239.1"
                     /translation="TIAARSGGADPASNARLRAGIDKAKAVSMPNDTIERALKRATGA
                     DADKMEEIRYEGYGPSGVALIIDCMTDNTQRTVADVRSTLSKHGGNLGTSGSVAFQFK
                     HVGELIVDTSKPGSEDKLLEAALDAGADDVQSEDGESIVLTSPENFEAVKKALAGAGF
                     APGKADVIWRPDNRTPVNAEVAETLRDLIDRLDELDDVQTVYHNAELAV"
     gene            complement(635..901)
                     /locus_tag="VFF05_10280"
     CDS             complement(635..901)
                     /locus_tag="VFF05_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006452846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HZW52240.1"
                     /translation="MATGWAGDNAVQDQIDATIKDAIKRVRGRAPKGPSLKRCEECGA
                     SIPEARRKAVPGVRLCVACQSERDEQERAFSGYNRRGSKDSQLR"
     gene            complement(974..>1301)
                     /locus_tag="VFF05_10285"
     CDS             complement(974..>1301)
                     /locus_tag="VFF05_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52241.1"
                     /translation="RIGSVHAIKLRLLAAVHGINGAGSRLDDVWRAWKNMPALPQALA
                     GRRGWTPEEIVGIESYAGMDARYYQPTLAEFRRSLGAESQELECAFSHNELGERCPTL
                     VLARRT"
BASE COUNT          228 a          449 c          424 g          200 t
ORIGIN      
        1 caccattgcc gcgcgcagcg gtggagccga tccggccagc aatgcgcgcc tgcgcgccgg
       61 catcgacaag gccaaggccg tctcgatgcc gaacgacacc atcgagcgtg cgctcaagcg
      121 cgcgaccggc gcggatgcgg acaagatgga ggaaatccgc tacgagggtt acggcccgtc
      181 cggcgtcgcg ctcatcatcg actgcatgac cgacaacacc cagcgcaccg tggccgacgt
      241 gcgcagcacg ctcagcaagc acggcggcaa cctcggcacc tcgggctcgg tcgcgttcca
      301 gttcaagcat gtcggcgagc tcatcgtcga tacgagcaag ccgggcagcg aggacaagct
      361 tctcgaagcc gcgctcgatg ccggcgccga cgacgtgcaa agcgaagacg gcgagagcat
      421 cgtgctgacg tcgccggaaa atttcgaagc ggtgaagaag gcgctggctg gcgccggatt
      481 cgcgccgggc aaggccgacg tgatctggcg cccggacaac cgcacgccgg tgaacgccga
      541 agtcgccgaa accctgcgcg acctcatcga ccggctggac gagctcgacg acgtgcaaac
      601 cgtctatcac aacgccgaac ttgcggtgta agtctcagcg caactggctg tccttgctgc
      661 cgcgccggtt gtagccgctg aaggcgcgtt cctgttcatc gcgttcggac tggcaggcca
      721 cgcacaggcg cacgccgggc acggccttgc gccgcgcctc gggaatcgag gcgccgcatt
      781 cttcgcaacg tttcaggctc gggcctttcg gcgcacggcc gcgcacgcgc ttgatcgcgt
      841 ccttgatcgt cgcgtcgatc tggtcctgca ctgcgttgtc gcctgcccag ccggtggcca
      901 tgaggattgt ccgctgaagt cgaccgaata atgcggccgc acaaggcgac gcacaaggcc
      961 cgggccgcac gaatcaggta cggcgcgcca acaccagcgt gggacagcgc tctcccagct
     1021 cgttgtggct gaaagcgcat tccagttcct gcgactcggc gccgaggctg cggcggaatt
     1081 ccgccagcgt cggctggtag taacgcgcat ccatgcccgc gtaggattcg atccccacga
     1141 tttcttccgg cgtccagcca cgccgtccag ccagtgcctg cggcaacgcc ggcatgtttt
     1201 tccaggcgcg ccacacgtcg tccaggcggc tgcccgcgcc gttgatgccg tggaccgcgg
     1261 cgagcagccg cagcttgatg gcgtgcacgc tgccgatgcg t
//
LOCUS       DASRVO010000832         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_207214, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000832 DASRVO010000000
VERSION     DASRVO010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207214"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFF05_10290"
     CDS             complement(<1..662)
                     /locus_tag="VFF05_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZW52242.1"
                     /translation="MLDPALLRGQLDSVAGQLAARGFALPKTQIEALEARRKAAQIQT
                     QELQNLRNTRSKAIGMAKAKGEDTAALMAEVAGIGDQLKANEQTLAEIQAEIATITLG
                     IPNLADASVPHGKDETENKEIHEWGTPRGFDFAVKDHVELGARHGWLDGEVAAKLSGA
                     RFTVLRGQLARLHRAIGQFMLDLQTREHGYTEVAPPLLVRDEAAFGTGQLPKFAEDLF
                     QTT"
     gene            complement(692..>1507)
                     /locus_tag="VFF05_10295"
     CDS             complement(692..>1507)
                     /locus_tag="VFF05_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZW52243.1"
                     /translation="ALLSLSVDASQRGLAFGFHRAMDNFGAVIGPLVAAAMLAAGMSL
                     RHVFLWAIVPGVVVIVLALFLKDDATPAPAHKPAAFSWNLREFPPAFRRYLIVLALFT
                     LGNSSNMFLLLRARDLGLPQAEVPVLWALVSLVAALLSTPLSSLSDRIGRTRLIVSGW
                     IVYAAFYLILGVQNDNSWALWPMFAGYGVFLAATEGAEKALVADLVPRESSGTAYGWY
                     NLVVGLFLLPASIVFGWLWQLETPMFAFGFGAGCAVIAALLLKFWVGPARAAI"
BASE COUNT          293 a          491 c          485 g          238 t
ORIGIN      
        1 gtggtttgga acaggtcctc ggcgaacttg gggagctggc cggtgccgaa cgccgcctcg
       61 tcgcgcacca gcagcggcgg ggcgacctcg gtgtagccgt gctctcgggt ctgcaggtcg
      121 agcatgaact ggccgatcgc ccgatgcagg cgggcgagct ggccgcgcag cacggtgaag
      181 cgcgcgccgg aaagttttgc cgccacttcg ccatcgagcc agccgtggcg cgcgccgagt
      241 tcaacatggt ccttcaccgc gaaatcgaaa ccacgcggcg tgccccactc gtggatttcc
      301 ttgttttcgg tttcgtcctt gccgtgtgga accgaagcgt cggccaggtt cgggatgccc
      361 agcgtgatcg tggcaatctc ggcctggatc tcggccagtg tctgctcgtt ggccttgagc
      421 tggtcgccga tgccggcgac ttcggccatc agcgcggctg tgtcctcgcc cttggccttg
      481 gccatgccaa tcgccttgga acgcgtgttg cgcaggttct gcagttcctg cgtctggatt
      541 tgcgcggctt tgcggcgcgc ttccagcgcc tcgatctgcg tcttcggcaa ggcgaatccg
      601 cgcgcagcca attgcccggc gacgctgtcg agttgtccac gcaggagtgc cggatcgagc
      661 atggtggaat tccacggaaa aagaggcgca attatatcgc agcgcgcgcc ggtcccaccc
      721 agaatttcag cagcagcgca gcgatcacgg cgcaacccgc accgaatccg aatgcgaaca
      781 tcggcgtttc gagctgccac aaccagccga acacgatgga tgccggcaac aggaacaagc
      841 cgacgacaag gttgtaccag ccgtaggccg tacccgagga ttcgcgcggc accaaatccg
      901 caaccagcgc tttttccgcg ccctcggtgg cggcaaggaa aacgccgtag ccggcgaaca
      961 tcggccacaa cgcccaggag ttgtcgttct gcacgccgag aatgagataa aaggcggcgt
     1021 agacgatcca gccggaaacg atcaggcgcg tgcggccgat gcgatccgac aaggacgaca
     1081 gcggcgtcga caacagcgcc gcgaccagcg acaccagcgc ccacagcacc ggcacttcgg
     1141 cctgcggcag gccgaggtcg cgcgcgcgca agagcaggaa catgttcgag gagttgccca
     1201 atgtaaacaa ggcaagcacg atcaggtaac gccggaacgc gggcgggaat tcgcgcaggt
     1261 tccacgaaaa tgcagctggc ttgtgtgctg gcgctggcgt ggcgtcgtcc ttcaagaaca
     1321 gcgccaacac gatcacgacg acacccggca cgatggccca caggaacacg tggcgcagcg
     1381 acattccggc ggccagcatc gccgccgcca cgagcggtcc gatcaccgcg ccgaagttgt
     1441 ccatcgcgcg gtggaacccg aacgcgagtc cgcgttgtga cgcatccacg ctcagcgaca
     1501 gcaacgc
//
LOCUS       DASRVO010000833         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_153596, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000833 DASRVO010000000
VERSION     DASRVO010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153596"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1968
                     /gene="htpG"
                     /locus_tag="VFF05_10300"
     CDS             94..1968
                     /gene="htpG"
                     /locus_tag="VFF05_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZW52244.1"
                     /translation="MTAAAETRPFEAEVAQVLHLVTHSLYSHKEIFLRELVSNASDAC
                     DKLRFEALGKPELLTGDTELRIDISVDKDARTITIRDNGIGMTREEVVANIGTIASSG
                     TRKFLEALSADKKADANLIGQFGVGFYSAFVVADKVTLVTRKASAAVGEGVRWESSGS
                     GEYTLETVDAAERGTAVTLHLKPDEDEFLQDWQVRSLIARYSDHIGFAIRMRKPDKDG
                     KATQEWEVVNQASALWTRPKVELKDEEYQAFYKHISHDFNDPLAWTHNRVEGNQNFTS
                     LLYLPAQAPFDLMLGGRDERKGLKLYVKRVFIMDAAEQLLPSYLRFVRGVVDSDDLPL
                     NVSREILQQNRNLEKLKSACTRRVLDLLDKLAKDEPEKFTAFWNTFGNVFKEGVAEDF
                     ANKERIAKLLRFASTKSDGAAQTVSFDDYIARMKAGQDAIYYITADGYAAAKGSPQLE
                     ALKANDVEVLLMVDRIDEWMSGYLNEYTGKKLRNVAKGDLELDKLGGASAEARKETEK
                     AAEGVVKKLKELLGERVSDVRVSTRLTDSPACIVLDEHDMGLAMQRLMKQAGHEVPAS
                     KPVLEINPAHALVKRLETDSAHAADLALLLLDQAQLLEGVALEDPAAYVRRVNALLTA
                     "
     gene            1976..>3659
                     /locus_tag="VFF05_10305"
     CDS             1976..>3659
                     /locus_tag="VFF05_10305"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW52245.1"
                     /translation="MSADDQRLALEQLHKGAALHRAGQIGLAQSHYQRAVKLDPHNAD
                     AWHLLGVAALQSGNPALAAKHLRTCVKTRPAFAEAHNNLGVALRQGGKHDEAIAAFRA
                     ALGVRGGYIDAMFNLGIAFEAAEKAGEADRCYRQVLAWRADHADAATNLGNLLRRHGR
                     LTEALPLAELAQRVAPDRAQTNGNLALLLSDLGRQFDAMRFARAALTQEPDQALWWKT
                     LGVAQRLLHDVDNAIPSLRRALELDRDDAATALELALALNEGGESDAARQLFAATTPP
                     SGFGERVRWLRALTLPAIYRDDAEIDAARAQFGDGLETLHAGLKLDTPVAVHEAVHAA
                     ASVAPFHLHYQPRDSTVLQCRFGDLVARVMSAAAPELAQPCDWQPRASGGRVRVGIVA
                     SHLMQHTVSRYFTRLIEGLDRERFDVRIWHGGGEDESTRRIAANVSTFVQTQADALTL
                     ARAVRDVRLDVLVYPEIGMDPKHQALAALRLAPVQCALYGHPATSGAPNIDYFLSGAE
                     LEPADADGHYREKLVRLPGLGTQPLPPPAPGDGAWLDAQASDAPLVLCLQNFI"
BASE COUNT          681 a         1168 c         1187 g          623 t
ORIGIN      
        1 gtgccgaatt ctgtcttcga cttgcggctt ctgtggttga attctctcca cttcggccat
       61 atccggttgg cattcacttc tcgagggaat cccatgaccg ccgctgctga aacccgtccg
      121 ttcgaagccg aagtcgccca ggttctgcac ctggtcacgc attcgttgta ttcgcacaag
      181 gaaatcttcc tgcgcgaact cgtatcgaac gcttccgatg cctgcgacaa gctgcgtttc
      241 gaggcgctcg gaaaacccga gttgctgacc ggcgacaccg agctgcgcat cgacatttcc
      301 gtcgacaagg atgcgcgcac gatcacgatc cgcgacaacg gcatcggcat gacgcgcgag
      361 gaagtcgtcg ccaacatcgg cacgatcgcc agttccggca cgcgcaagtt cctcgaggcc
      421 ctttccgcgg acaagaaggc cgacgccaac ctgatcggcc aattcggcgt gggtttctac
      481 tcggccttcg tcgttgccga caaggtgacg ctggtgacgc gcaaggccag cgcggcggtg
      541 ggcgagggcg tgcgctggga gtcgtcgggc agcggcgaat acacgttgga gaccgtggat
      601 gcggccgaac gcggcaccgc ggtcacgctg catctcaagc ctgacgaaga cgagttcctg
      661 caggactggc aggtgcgcag tcttatcgcg cgctactcgg atcacatcgg tttcgctatc
      721 cgcatgcgca agccggacaa agacggcaag gccacacaag aatgggaggt cgtgaatcag
      781 gcgtccgcgt tgtggacacg gcccaaggtc gagctgaaag acgaggaata tcaggcgttt
      841 tacaagcata tctcgcacga tttcaacgac ccgctggcat ggacgcacaa ccgcgtcgag
      901 ggcaaccaga atttcacttc gctgctgtat ctgcccgcgc aggcgccgtt cgacctgatg
      961 ttgggcgggc gcgacgagcg caagggcctg aagctttacg tcaagcgcgt cttcatcatg
     1021 gacgcggccg aacaactgct gccgtcgtat ctgcgcttcg tgcgcggcgt ggtcgattcg
     1081 gacgacctgc cgctcaacgt cagccgcgaa atcctgcagc agaaccgcaa tctggaaaag
     1141 ctcaagtctg cctgcacgcg ccgcgtgctc gacctgctcg acaaactggc gaaagacgag
     1201 ccagaaaaat ttaccgcgtt ctggaacacg ttcggcaacg tgttcaagga aggcgtggcc
     1261 gaggatttcg cgaacaagga gcgcatcgcc aagctgctgc gcttcgcctc gacgaaatcc
     1321 gatggcgcgg cgcagacggt ttcgttcgac gattacatcg cccgcatgaa ggcgggacaa
     1381 gatgcgatct actacatcac cgccgacggc tacgccgccg cgaagggctc gccgcagctc
     1441 gaagcgttga aggcgaatga cgtcgaagtt ctgctgatgg tcgatcgcat cgacgaatgg
     1501 atgtcgggct atctgaatga gtacaccggc aagaaactgc gcaatgtcgc caagggcgat
     1561 ctggaactgg acaagctcgg cggcgccagt gcggaagcac gcaaggagac cgagaaggcc
     1621 gccgagggcg tggtgaaaaa gctgaaggaa ctgctcggcg agcgcgtgag cgacgtgcgc
     1681 gtgtccacgc gcctgaccga ttcgccggcc tgcatcgtgc tggacgagca cgacatgggc
     1741 ctggccatgc agcgcctgat gaagcaggcc ggacacgaag tgccggcgtc gaagcccgtg
     1801 ctggaaatca atccggcgca tgcgctggtc aagcggctcg aaaccgattc cgcgcacgcc
     1861 gccgatctcg cgcttctgct gctcgatcag gcgcagttgc tcgaaggcgt ggcgctggag
     1921 gatccggccg cctatgtgcg tcgcgtcaat gcactgttga ccgcatgatc gcgacatgag
     1981 cgccgacgac cagcgcctgg cgctcgagca actgcacaag ggcgcggcgc tgcatcgtgc
     2041 gggccaaatt gggctggcgc agagccatta ccagcgcgcg gtcaagctcg atccgcacaa
     2101 cgccgacgcc tggcatctgc tcggcgtcgc ggcgttgcaa tcgggcaatc ccgcgctcgc
     2161 agccaagcat ctgcgcactt gcgtgaaaac gcgtccagcg tttgcggagg cgcacaacaa
     2221 cctcggcgtt gcgctgcgcc agggcggcaa gcacgatgaa gccatcgcgg cgttccgtgc
     2281 ggcgctcggt gtgcgcggcg gttacatcga cgcgatgttc aatctcggca ttgcgttcga
     2341 agccgcggaa aaggcgggcg aagccgaccg ttgctatcgc caggtgcttg cctggcgcgc
     2401 ggatcacgcc gatgccgcga ccaacctggg caatctcctg cgccggcatg gccgtctgac
     2461 cgaagccttg ccccttgccg aactcgccca gcgcgtggcg ccggatcgcg cgcagaccaa
     2521 cggcaatctc gcgctgctgc tttccgatct cggtcgccag ttcgacgcca tgcgcttcgc
     2581 gcgcgccgcg cttacgcagg agcctgatca agccttgtgg tggaagactc tgggtgtcgc
     2641 gcagcgcctg ctgcacgacg tcgacaatgc gattccgagc ttgcgccgtg cgctcgaact
     2701 cgatcgcgac gatgcggcga cggcgctgga actggcgctc gcgctcaacg aaggcggcga
     2761 atccgatgcc gcgcggcaac tgtttgccgc gacgacgccg ccatcgggat tcggcgaacg
     2821 cgtgcgctgg ctgcgcgcgc tgaccctgcc cgcgatctac cgcgacgacg cggaaatcga
     2881 cgcggcgcgc gcgcaatttg gcgacggact cgaaacgctg catgcgggat tgaagctcga
     2941 tacgcccgta gcggtccacg aagccgtgca tgccgcagcc agcgtggcgc cgttccactt
     3001 gcattaccag ccgcgcgaca gcacggtcct gcaatgccgc tttggcgatc tcgttgcgcg
     3061 agtcatgtcc gccgccgcgc cggaactcgc gcagccctgc gactggcagc cgcgggcgtc
     3121 cggtgggcgt gtgcgcgtcg gcatcgtcgc gagccacttg atgcaacaca cggtgtcgcg
     3181 ctatttcacg cgcctgatcg agggattgga tcgcgagcgc ttcgacgtgc gcatctggca
     3241 tggcggcggc gaggacgaga gcacgcgcag gatagcggcg aacgtttcca cattcgtgca
     3301 gacgcaggcc gatgcgctca cgctcgcacg cgcggtgcgc gatgtgcggc tcgacgtgct
     3361 cgtctatccc gaaatcggca tggatccgaa acatcaggca ttggccgcgc tgcgcctcgc
     3421 gccggtgcag tgcgcgctgt acggccatcc ggccacgagc ggtgcgccga acatcgacta
     3481 ttttcttagt ggcgcggaac tggaaccagc cgacgcggat gggcattacc gcgagaagct
     3541 cgtgcgcctg ccgggactcg gcacgcagcc gctgccgccg cctgcgccag gcgatggcgc
     3601 gtggctcgat gcgcaggcca gtgatgcgcc gctcgtgctg tgcctgcaga acttcatca
//
LOCUS       DASRVO010000834         1115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_76779, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000834 DASRVO010000000
VERSION     DASRVO010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76779"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1115
                     /locus_tag="VFF05_10310"
     CDS             <1..>1115
                     /locus_tag="VFF05_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZW52246.1"
                     /translation="RVLERRPEGPQTPLYATTIAHLAASDLGELWQGAMFLTEKQGGS
                     DVGANAVTATRHGERWLLNGDKWFCSNVTADAILALARMPGNTPGTRGLGLFLVLRRK
                     PAGNTGTIRIHRLKDKLGVRSMATGEVTFENTQAYLIAGENEGFKQMAEMLNLSRLYN
                     AVASVAGMRRALLEALAYGAGRKAFGHALNELPLWRAAMADLVAEHLGIFTLVFETVR
                     ALDRADNDDDAARRFVRLAIPMAKALSGKLAVFAVSEAMEAIGGNAYIEESILPRLLR
                     DCQVLPIWEGTTHILTLDALRAIHKEGSHEALFSRIGLALARANKSGTGPVKARLECD
                     IRQLDALLKLPPELQQREARLWLETAGRTLTLALLLE"
BASE COUNT          221 a          357 c          361 g          176 t
ORIGIN      
        1 cgtgtgctcg agcgcaggcc cgaagggccg cagaccccgc tctatgcaac gacgatcgcg
       61 catcttgccg ccagcgatct cggcgaactg tggcagggcg cgatgttcct gaccgagaag
      121 cagggcggtt cggatgtggg cgccaatgcc gtcacggcaa cgaggcatgg cgaacgctgg
      181 ctgctcaacg gcgacaagtg gttctgctcc aacgtgaccg ccgacgccat cctcgcgctg
      241 gcgcgcatgc ccggcaatac tcccggcacc agggggctcg gactctttct cgtgctgcgc
      301 cggaaacccg caggcaacac gggcacgatc cgcatccaca ggctcaagga caaactcggc
      361 gtgcgctcaa tggcgacggg cgaagtgacc ttcgagaaca cccaggccta cctgatcgca
      421 ggcgagaacg aaggcttcaa gcagatggcc gaaatgctga acctgtcgcg actgtataac
      481 gccgtcgcga gcgtcgccgg tatgcgccgg gcgctgctgg aagcgctcgc gtacggcgcc
      541 gggcgcaagg ctttcgggca cgcactcaac gaactgccgc tctggcgcgc cgcgatggcc
      601 gaccttgtgg ccgaacacct gggcatcttc acgctcgtct tcgaaaccgt gcgcgcactg
      661 gaccgcgccg acaacgacga tgacgccgcc cgcaggttcg tacgcctcgc aatacccatg
      721 gccaaggcat tgagcggcaa gctcgcggtt tttgcggtga gtgaagcgat ggaggcgatc
      781 ggcggcaatg cctatatcga ggaaagcatt cttccgaggc tgttgcgcga ctgccaggtt
      841 ctgccgatct gggaaggcac gactcacatc ctgacgctgg acgcgttgcg cgcgatccac
      901 aaggaaggga gtcacgaagc attgttctcg cgaatcgggc tggcattggc acgcgcgaat
      961 aaatcgggca ccggaccggt caaagccagg ctggaatgcg acatccggca actcgatgcg
     1021 ctgttgaagc tgcctcccga actgcaacag cgcgaggcgc ggctatggct ggaaaccgcc
     1081 ggccggactc tcacgctggc gctgctgctc gaaaa
//
LOCUS       DASRVO010000835         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_361453, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000835 DASRVO010000000
VERSION     DASRVO010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361453"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFF05_10315"
     CDS             complement(<1..834)
                     /locus_tag="VFF05_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HZW52247.1"
                     /translation="MNARIAAPELPTSLDWVNTDTPPNLAALRGRVTLIHFWTFDCVN
                     CRNVLPDLRYLENKYHDGLSIIGVHTPKFEYQRHGEPVLKAVNRNHIRHPVANDPDYL
                     LWQAYGVDSWPTLAVIDAQGQLAALLPGEGRRQEADTLIAHLLDEAAAQDLRVYESDV
                     ATARPEARAPLRFPSRLAVGESSLWISDSGHNRILECTHEGHILRQYGSGNPGYWDGR
                     GKDAGFTDPQGLALGKDMLFVADTGNHAVRRVRLLSSDVDTVAGTGVAGHDLPNESLE
                     PA"
     gene            complement(866..1639)
                     /gene="kdsB"
                     /locus_tag="VFF05_10320"
     CDS             complement(866..1639)
                     /gene="kdsB"
                     /locus_tag="VFF05_10320"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HZW52248.1"
                     /translation="MADTLNFIVAIPARYASTRLPGKPLRQIAGEPMILRVAQRALAA
                     GATEVVVATDDARIADALRDAPVRVVMTRTDHASGSDRLAECAEKLRWADDTIVVNLQ
                     GDEPLAPPSGIRAVAECLATGNAPMATLATPIGTARELFDPNCVKVVAGLDGHAVYFS
                     RAPLPWARDAFARTQDVLPAEPVFLRHIGIYAYRAGFLRAFAGLAPTLAERAEALEQL
                     RAIEHGHRIAVRLAPEPFPAGVDTPADLARVDALVRAGA"
BASE COUNT          294 a          555 c          574 g          290 t
ORIGIN      
        1 cgccggctcc aggctttcgt tcggcagatc gtgaccggcc acaccggtac cggcgaccgt
       61 atccacgtcg ctgctgagca ggcgcacgcg gcggaccgcg tgattgccgg tgtcggcgac
      121 gaagagcatg tccttgccca aggccagacc ctgcggatcc gtgaacccgg catccttgcc
      181 gcggccgtcc cagtatcccg gattgcccga accgtactgg cgcaggatgt gaccttcgtg
      241 cgtacattcc aggattcggt tgtgtccgct gtcggaaatc cacaggctcg attcgcccac
      301 cgccaaacgc gacggaaagc gcaacggcgc gcgcgcttcc ggacgcgccg tggcgacatc
      361 ggattcgtac acgcgcagat cctgcgcggc ggcttcgtcg agcagatgcg caatcagggt
      421 gtcggcttcc tgacggcgac cttcgccggg aagcaacgcg gcgagctggc cctgcgcgtc
      481 gatcaccgcc agcgtcggcc aggaatccac gccgtaggcc tgccacagca gatagtccgg
      541 gtcgttcgcg accggatgcc ggatatggtt gcgattgacc gccttgagca ccggctcgcc
      601 gtggcgctga tactcgaact tgggcgtatg cacgccaatg atgctcagac cgtcgtggta
      661 cttgttttcg aggtagcgca ggtccggcag gacattgcgg cagttgacgc aatcgaacgt
      721 ccagaaatgg atcagggtca cacgcccgcg cagcgcggca aggttgggtg gcgtatcggt
      781 attcacccaa tcgagcgacg ttggcaactc cggtgcggcg attctggcat tcatgcgcgg
      841 cgtggattcg tgatgatggt cgccattatg cgccagcgcg caccagcgca tcgacccgcg
      901 caaggtccgc gggtgtgtcc acgccggccg gaaacggctc cggcgccaga cgcacggcaa
      961 tgcgatggcc gtgttcgatc gcgcgtaatt gctccagcgc ttcggcacgt tcggccagcg
     1021 tcggtgccag tccggcgaac gcgcgcagga atccggcgcg atacgcgtaa atgccgatat
     1081 ggcgcaggaa cacgggttcg gcgggcagca cgtcctgggt gcgcgcaaac gcatcgcgtg
     1141 cccacggcag cggcgcgcgg gaaaaatata ccgcgtggcc atcgagcccg gccacgacct
     1201 tgacgcagtt cggatcgaac agttcgcgtg cggttcctat gggcgtggcg agcgtggcca
     1261 ttggagcgtt tcccgttgcc aggcactcgg cgaccgcacg gatgccgctc ggcggggcca
     1321 gcggttcgtc gccctgcaaa ttcaccacaa tggtatcgtc cgcccagcgc aacttttccg
     1381 cgcattccgc cagccggtcg ctgcccgagg cgtgatcggt gcgcgtcatg accacgcgca
     1441 cgggcgcatc gcgcaatgca tcggcgatgc gcgcatcgtc cgtggcgacg acgacttccg
     1501 tggcgccagc ggccagggcg cgttgtgcca cgcgcaggat catcggctcg ccggcgattt
     1561 gccgcagcgg cttgcccggc aaccgcgtgg acgcgtaacg cgcgggaatg gcaacgatga
     1621 aattaagcgt gtcggccatg gcagttctgg aaatcccgtc gcgagcctac tgaagtgtcg
     1681 cgacgaaaga tacctgccgg cagtcggtag gcg
//
LOCUS       DASRVO010000836         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_387967, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000836 DASRVO010000000
VERSION     DASRVO010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387967"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..392)
                     /gene="infA"
                     /locus_tag="VFF05_10325"
     CDS             complement(174..392)
                     /gene="infA"
                     /locus_tag="VFF05_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002553999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZW52249.1"
                     /translation="MAKDDQIEMEGTVLDTLPNTMFRVQLENGHIVTAHISGRMRKNY
                     IRILTGDKVKVEMTPYDLTKGRITYRMK"
     gene            complement(475..>1096)
                     /gene="aat"
                     /locus_tag="VFF05_10330"
     CDS             complement(475..>1096)
                     /gene="aat"
                     /locus_tag="VFF05_10330"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009601450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZW52250.1"
                     /translation="SPERLLDAYRHGIFPWYSHGQPILWWSPDPRAVFATNRVHVSTR
                     LKRWLRHCDWTIAADRNFAGVMRACAAPRATQTGTWITAEMYSAYCWLHSLGHAHSVE
                     VYAGNTLAGGIYGVAVGRMFFGESMFSAETNGSKVALIALCRVLREWGFTLLDAQVAS
                     EHLATLGAIQMPRSEFVAHVAKACAEPFASGPWRERWPLLSAHELV"
BASE COUNT          208 a          322 c          365 g          201 t
ORIGIN      
        1 cgccgacggc ggcagggtct ggctgggtgt caaggatggc cagctcgacg tgcgtatcga
       61 agccgaagaa aagctgccgg ccgttgtcga gtaattcggg tcgagcaaat tacggcgata
      121 atgaaagcaa aacggcggcc atgggccgcc gttttcatgt cagtgcaccg cagttatttc
      181 atgcgatacg tgatccgccc cttggtcaga tcgtagggcg tcatttccac cttgaccttg
      241 tcgccggtca ggatgcggat gtaattcttg cgcatgcgcc cggaaatatg tgcggtcacg
      301 atgtggccgt tttccagttg cacgcggaac atggtgttgg gcagggtgtc gagcaccgtg
      361 ccttccattt cgatttggtc gtcttttgcc attggctcac tgatcgggga ctgggctttc
      421 cctgcggcgc ggcattctac acgatagcga cccggcgtga gcgacgagcg cgcttcagac
      481 caattcgtgc gccgacaaca atggccagcg ttcgcgccac ggcccggacg caaacggctc
      541 cgcgcaggcc ttggcgacat gcgcgacgaa ctcgcttcgc ggcatctgga tagcgcccag
      601 cgtcgccagg tgctccgagg cgacctgggc gtcgagcagg gtgaaacccc attcgcgcag
      661 gacccggcac agggcgatca gggcgacttt cgagccattg gtttcggcgc tgaacatgga
      721 ttcgccgaag aacatgcgcc cgaccgcgac gccgtagatg ccgccggcca gcgtgttgcc
      781 ggcgtaaacc tcgaccgagt gcgcgtggcc aagcgagtgc aaccagcagt aggccgagta
      841 catttcagcc gtgatccagg tgccggtttg ggtagcgcgt ggagcggcac aggcgcgcat
      901 cacgccggcg aagttgcggt cggccgcaat cgtccaatcg caatgccgca accagcgttt
      961 caggcgcgtc gaaacgtgga cacgattggt cgcgaacacg gcgcgcgggt cgggcgacca
     1021 ccaaaggatc ggctgtccgt gcgaatacca ggggaaaatc ccgtgccggt aggcgtcgag
     1081 aaggcgttcg ggcgaa
//
LOCUS       DASRVO010000837         1588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_153664, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000837 DASRVO010000000
VERSION     DASRVO010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153664"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VFF05_10335"
     CDS             complement(<1..301)
                     /locus_tag="VFF05_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZW52251.1"
                     /translation="MTQRLHFLVAGGGIGGLAAAYVLAGDGHAVTVLEQSSAFGEIGA
                     GIQLGPNIFRMFEYLGLADAVDRVAFFPPGMVMNDVRTGERVVRVALGDAARAAYG"
     gene            467..1153
                     /locus_tag="VFF05_10340"
     CDS             467..1153
                     /locus_tag="VFF05_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HZW52252.1"
                     /translation="MKGKAMKRRLQMRPAAYAAVAVLALAGCADMDMGNMSPTAQHTA
                     IGAGVGAAAGAVLGGDTRSAAIGGLVGAAGGYIWSRHMQDKKAAMERATAGSGVQVTQ
                     TADNQLKLNIPSDVSFDTGRADIKPNLRPILDQFAHGLSTQPNTEVRIIGHTDSTGTP
                     AINDPLSVQRAQSTKNYLIARGVDPNRIIIAGRGDREPIADNSTEAGRARNRRVEIFL
                     GERANIATTR"
     gene            complement(1240..>1588)
                     /locus_tag="VFF05_10345"
     CDS             complement(1240..>1588)
                     /locus_tag="VFF05_10345"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HZW52253.1"
                     /translation="PALRLTTRRTHMILELADIRIQPGQQAAFEDAIRRGVDTVASKA
                     QGMRGYKINRCIENRERYVLQIFWDRLEDHTVTFRQGPLFTQWRAIVGPFFAQPPHVE
                     HFELVAKSAEVIG"
BASE COUNT          279 a          514 c          556 g          239 t
ORIGIN      
        1 agccgtaagc cgcacgtgcc gcgtcaccga gcgccacgcg cacgacgcgc tcgcccgtgc
       61 gcacgtcgtt catgaccatg ccgggcggga agaaggcgac ccggtccacc gcgtccgcga
      121 ggccgaggta ctcgaacatg cgaaagatgt tcgggccaag ctggatgccg gcgccgattt
      181 cgccgaaggc ggacgactgc tccagcaccg tcacggcgtg gccgtccccg gcgagcacgt
      241 acgcggccgc gaggccgccg atgccgccac cggccaccag gaaatgaagg cgctgcgtca
      301 tgggttcgaa tcggttgcgc gagaaaacgg ccatgctacc tggaccgtcg cgacccgacg
      361 cgccggtggg acgatcggtt cgtgccgccg cgcagcatac aaaaagttac gtgctcggct
      421 gtttgagctt tgcccggcgg tgtccttgaa tgatgagtgt ctctcagtga aaggcaaggc
      481 gatgaaaaga cgattgcaga tgaggccggc ggcgtacgcg gcggtggccg tcctggcgtt
      541 ggccggttgt gccgacatgg acatggggaa catgtccccg acggcgcaac acacggcgat
      601 cggtgcgggc gtcggcgccg ctgccggggc cgtgctcggc ggcgatacac gcagcgccgc
      661 gattggcggc ctggtcggtg ccgccggtgg gtacatctgg tccaggcaca tgcaggacaa
      721 gaaggccgcg atggagcgtg cgaccgccgg gtccggcgtg caggtgacgc aaaccgcgga
      781 caaccagttg aagctgaaca tcccgagcga cgtttcgttc gacaccggcc gcgccgacat
      841 caagcccaac ctgcggccga tcctggatca gttcgcgcac ggcctgtcga cgcagccgaa
      901 caccgaggtg cggatcatcg gtcacaccga cagcacgggc acgccggcga tcaacgatcc
      961 gctgtctgtg cagcgggcgc agtcgacgaa gaactacctg atcgcgcgcg gggtcgatcc
     1021 gaaccggatc atcatcgccg gccgcggtga ccgcgaaccg atcgcggaca acagcaccga
     1081 agcgggccgt gcgcgcaacc gccgggtcga gatcttcctt ggcgagcggg ccaacatcgc
     1141 gaccacgcgt tgatggcggc cgcgccggtg cacaggcggc cttttcatcg atgcgccagg
     1201 catggcgcgc agcgccgtca ctggcgctgt ctggctcgcc tacccgatca cctcggccga
     1261 cttggcaacg agctcgaaat gctctacgtg gggcggctgc gcgaaaaacg ggccgacgat
     1321 cgcgcgccac tgcgtgaaca gcgggccctg gcggaacgtg acggtgtggt cttccaggcg
     1381 atcccagaag atctgcagga cgtagcgctc gcggttttcg atgcagcggt tgatcttgta
     1441 gccgcgcatg ccctgcgcct tcgaggcgac ggtgtccacg ccgcggcgga tggcgtcctc
     1501 gaaagcggcc tgctggccgg gctggatgcg gatgtcggcg agttccagaa tcatgtgcgt
     1561 ccttcgcgtg gtcaatcgca gtgcaggg
//
LOCUS       DASRVO010000838         1005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_336925, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000838 DASRVO010000000
VERSION     DASRVO010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336925"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /gene="uvrC"
                     /locus_tag="VFF05_10350"
     CDS             complement(<1..256)
                     /gene="uvrC"
                     /locus_tag="VFF05_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrC both incises the 5'
                     and 3' sides of the lesion. The N-terminal half is
                     responsible for the 3' incision and the C-terminal half is
                     responsible for the 5' incision; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit C"
                     /protein_id="HZW52254.1"
                     /translation="MTQVQGRQDINVAGMGDADVIAAHMAAGHASVQVFFFRGGQNWG
                     NRAYFPRAEKTFTAEEVLGSFLAQFYDDKPPPKLILLSHEI"
     gene            complement(253..>1005)
                     /locus_tag="VFF05_10355"
     CDS             complement(253..>1005)
                     /locus_tag="VFF05_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZW52255.1"
                     /translation="TDTAVDETVPATGQLGQGIAAIRAVLRTLPSSPGVYRMLNAKGE
                     PIYVGKASNLKNRVANYTHIGNLSNRLRRMVAETRAMEVVLTHTEVEALLLECNLIKR
                     FLPRYNVLLRDDKSFPYIHLTGDTDFPQLTKHRGATGESGDYFGPFASAGAVNRTLIA
                     LQRAFLLRSCSDSIFSSRTRPCLLYQIKRCSAPCVGRIDKEGYDRLVGEARDFLRGRT
                     KDVQEQLSARMTQMSRRSTSRARRCCAIASAA"
BASE COUNT          177 a          308 c          347 g          173 t
ORIGIN      
        1 cgatctcatg cgagagcagg atcagtttcg gcggcggctt gtcgtcatag aattgggcga
       61 ggaacgaacc gagcacctct tccgccgtga aggtcttttc ggcgcggggg aaataggcgc
      121 ggttgcccca attctgcccg ccgcggaaga agaacacctg gaccgaggca tggccagccg
      181 ccatatgcgc cgcaatcaca tcggcatcgc ccattcccgc gacattgatg tcctgccggc
      241 cttgcacctg cgtcaagccg cggatgcgat cgcgcagcag cgccgcgccc tcgaagtcga
      301 gcgcctcgac atttgcgtca tgcgtgccga gagctgctcc tggacgtcct tggtgcggcc
      361 gcgcaggaag tcccgcgcct cgccgacgag gcggtcatag ccctccttgt cgatgcggcc
      421 gacgcaaggc gcgctgcagc gcttgatctg atagagcagg cagggccgcg tgcggctcga
      481 gaaaatgctg tcggagcagg agcgcagcag aaacgcgcgc tgcaaggcga tgagcgtgcg
      541 attgaccgcg ccggccgagg cgaaggggcc gaagtaatcg ccgctttcgc cggtcgcgcc
      601 gcgatgcttg gtcagctgcg gaaaatccgt atcgccggtg agatggatat aggggaacga
      661 cttgtcgtcg cgcagcagca cgttgtagcg cggcaggaag cgcttgatga gattgcattc
      721 gagcagcagc gcctcgacct cggtgtgggt cagcaccact tccatcgcgc gcgtctcggc
      781 gaccatgcgc cgcagccggt tcgagaggtt gccgatatgg gtgtagttgg cgacgcgatt
      841 cttgagattg ctggccttgc cgacatagat cggctcgccc ttggcattga gcatgcggta
      901 gacgccgggg gaggacggca aggtgcgcag caccgcgcgg atcgccgcga tgccctggcc
      961 gagctgacca gtcgccggca cggtctcgtc cacggcggta tcggt
//
LOCUS       DASRVO010000839         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_361530, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000839 DASRVO010000000
VERSION     DASRVO010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361530"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /locus_tag="VFF05_10360"
     CDS             <1..1353
                     /locus_tag="VFF05_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002803093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZW52256.1"
                     /translation="GSLVGFIAAWANWIAIVSVIPVEAEASVQYMASWPWAWTQALYV
                     PAPGGRGELTPPGLTIAAALVIVYFLLNFWSVKVFARSNTAITIFKLVVPALTGLALI
                     ASGFHADNFSVGIHSGPHTTDWAAVLTAVAIAGIVFSYNGFQSPVNMAGEARNPGRSV
                     PLAVIGAIGLATIVYLLLQIAYIGAVPPDMLAKYGWHGIDFRSPFAQLALIVNLQWLA
                     MLLYADAFISPSGTGMTYTATCARMLYAMQKNGTMPAAFGTVHPRWGVPRAAMWFDLA
                     VGFVFLFFFRGWGTLAAVISGATIISYLTGPVSATVLRRTAPEIHRPLRLPALNALSC
                     LAFVFSTELMYWATWPLCGQIVLLVVIGLPIYFYYQARGRWQDFRRQIDGAWWLIAYL
                     PAISALSYVGSTKFGGLGWMPYGWDLVIVGLFGLVFYFWGLACGWRTPDVEAVRQSVG
                     "
     gene            1418..>2086
                     /locus_tag="VFF05_10365"
     CDS             1418..>2086
                     /locus_tag="VFF05_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZW52257.1"
                     /translation="MSRALFAALAVAFGIAGARTALAAPSDDWNAPYLLAGTATAQVE
                     PAAPAATFYPRTESPLSFDYLKLIWSDAVETATEPARWDGDDWRKAALVGGGLIFSMA
                     VLDKPVRDAAQRSRSKSADRFFRDVQKFGTKQYGLPVLLGFYGYGALADDNEAKAVAL
                     DGFSASVISALVTSSIKGVVGRARPYTGLGPHHFAPLQGDYSFPSGHATGAFAFASVI
                     ATHYD"
BASE COUNT          306 a          699 c          665 g          416 t
ORIGIN      
        1 ggctcgctgg tcggcttcat cgcggcctgg gccaactgga tcgcgatcgt ctcggtgatt
       61 cccgtcgaag ccgaagcctc ggtgcagtac atggcctcat ggccgtgggc atggacgcag
      121 gccctgtatg tgccggcgcc gggcggccgc ggcgaactga cgccgcccgg attgaccatc
      181 gcggccgcat tggtaattgt atacttttta ctgaatttct ggagcgtgaa ggtgttcgcg
      241 cgctccaaca cggcgatcac gatcttcaag ctcgtggtgc ccgcgctgac cgggctcgcg
      301 ctgatcgcga gcggattcca cgcggacaat ttctccgtcg gcatccacag cggtccgcac
      361 acgacggatt gggccgcggt cctcacggca gtagccatcg ccggcatcgt gttcagctac
      421 aacggtttcc agagcccggt gaacatggcc ggcgaggcgc gcaatcccgg gcgcagcgtg
      481 cccctggccg tgatcggcgc gatcggcctc gccaccatcg tgtatctgct gctgcaaatc
      541 gcctacatcg gcgcggtgcc gccggacatg ctggcgaaat acggctggca cggcatcgat
      601 ttccgttcgc cgttcgcgca actggcgctg atcgtgaacc tgcagtggct ggcgatgctg
      661 ctgtatgccg acgccttcat aagcccgagc ggcaccggca tgacctacac cgcgacctgc
      721 gcgcgcatgc tctacgcgat gcagaagaac ggcaccatgc cggcggcgtt cggcaccgtg
      781 catccgcgtt ggggcgtgcc gcgcgcggcg atgtggttcg acctcgccgt cggcttcgtt
      841 ttcctgttct tcttccgcgg ctggggcacc ctggcggcgg tgatttccgg cgccaccatc
      901 atctcttacc tgaccggacc ggtgagtgcg accgtgttgc gccggaccgc gccggaaata
      961 catcgaccgt tgcgcctgcc ggcattgaac gcgctgtcgt gcctggcctt cgtgttctcc
     1021 accgaactca tgtactgggc gacctggccg ctgtgcgggc agatcgtcct gctcgtcgtc
     1081 atcggcctgc cgatctattt ctattaccag gcacgcggtc gctggcagga ttttcgccgc
     1141 cagatcgacg gcgcgtggtg gctgatcgcc tacctgccgg cgatctcggc actgtcgtac
     1201 gtcggcagca cgaaattcgg cggactcggc tggatgccct acggctggga cctcgtcatc
     1261 gtcggcctgt tcggccttgt gttctatttc tggggattgg cctgcggttg gcgcaccccc
     1321 gatgtggaag cggtgcggca atccgttggc tgatttttgg cctcgataac agcgacggga
     1381 gtctaatcgc gtcaccgtcc cgactcgcgg gtgagtcatg tcgcgtgcat tgttcgcggc
     1441 gcttgccgtt gctttcggaa ttgccggtgc gcgcacggcg ctcgcggcac cgagcgacga
     1501 ctggaatgcg ccgtacctgc ttgccggcac tgcaacggcc caagtcgagc ctgccgcacc
     1561 ggctgcgacc ttctacccgc ggacggaaag cccgctgtcg ttcgattacc tcaagctgat
     1621 ctggtccgat gcggtggaaa ccgcgacgga acccgcgcgt tgggacggcg acgactggcg
     1681 caaggcagca ctggtcggcg gcggattgat cttcagcatg gccgtactcg acaaacctgt
     1741 tcgcgacgcg gcgcagcgca gccgctcgaa gtccgccgac cgcttcttcc gcgacgtcca
     1801 gaaattcggc accaagcagt acggcttgcc cgtcctgctc ggcttctacg gctacggcgc
     1861 gctcgccgac gacaacgagg ccaaggccgt cgcgctcgac ggattctccg ccagcgtgat
     1921 cagcgcgctg gtgacctcct cgatcaaggg cgtggtcggc cgcgcgcggc catataccgg
     1981 cctcggcccg caccatttcg cgccgttgca gggcgattac tcgtttccgt caggccatgc
     2041 caccggtgcg ttcgcgttcg cttccgtcat cgccacgcac tacgac
//
LOCUS       DASRVO010000840         1377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_102341, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000840 DASRVO010000000
VERSION     DASRVO010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102341"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VFF05_10370"
     CDS             complement(<1..469)
                     /locus_tag="VFF05_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidyl carboxypeptidase II"
                     /protein_id="HZW52258.1"
                     /translation="MFRIRLLPVAMTAVLAACAQAPQKPEPAKAAAVAPAPQHVNPFL
                     AASTLPFQAPPFDKITDADYQPAIEEGMRQQLAEVGGIANDPAPPTFENTYVALEKTG
                     ALLTRVMTAFNGVSGANTNDTLQALQETEAPKLAAHQDAIYLNGKLFARIDAVY"
     gene            complement(521..868)
                     /locus_tag="VFF05_10375"
     CDS             complement(521..868)
                     /locus_tag="VFF05_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52259.1"
                     /translation="MSVQRYYLSIDDLARARGQINELSFQGNSPDSFAALLQSALREP
                     TLWRRWRAMQPDPDAVDPALGASDPQATVSAEQADMHTDIVVTTSLPHAILKHRLTLL
                     VGAHWSLRDVKTA"
     gene            997..>1377
                     /locus_tag="VFF05_10380"
     CDS             997..>1377
                     /locus_tag="VFF05_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52260.1"
                     /translation="MSQRRLQLAATLVLCASTPFAFAGTQLLRFPDVCGDRIAFTYAG
                     DLWTASTQGGTATRLTAGPGLEQSARFSPDCKTIAFTGEYGGDDQVYTIPAYGGEPKQ
                     LTFYPSKGPLPQRWGFDSQVYGWTP"
BASE COUNT          222 a          434 c          471 g          250 t
ORIGIN      
        1 tgtacacggc gtcgatgcgc gcgaagagct tgccgttgag atagatcgcg tcctggtgcg
       61 cggccagctt cggcgcttcg gtttcctgca gcgcctgcaa ggtgtcgttg gtgttcgcac
      121 cgctgacgcc gttgaatgcg gtcatcacgc gggtgagcaa cgcgccggtt ttttccagcg
      181 cgacataggt gttctcgaag gtcggtggcg ccgggtcgtt cgcgatcccg ccgacctcag
      241 cgagttgctg gcgcatgcct tcctcgatcg ccggctggta atcggcgtcg gtgatcttgt
      301 cgaacggcgg cgcctggaac ggcaacgtgc tggccgcgag gaacggattg acgtgttgcg
      361 gcgcgggcgc gaccgcggcg gccttggccg gttcgggttt ctgcggcgct tgcgcgcagg
      421 cggcgagcac ggcggtcatg gcgacgggca gcaaacggat tcgaaacatg gcaagaactc
      481 cacgaagtat acgaatggaa aaaacaacgg attcacgcgg tcacgcggtt ttaacgtcgc
      541 gcaaactcca gtgcgcaccg accagcaggg tcagacggtg tttgaggatg gcatgcggca
      601 ggctggtggt gacgacgatg tcggtgtgca tgtcggcctg ttcggcgctg accgtggctt
      661 gcggatcgct cgcgccgagc gcgggatcga cggcgtccgg atccggctgc atcgcgcgcc
      721 agcgccgcca caacgtgggc tcgcgcaatg ccgattggag cagggcggcg aagctgtcgg
      781 gcgaattgcc ctggaacgac aattcgttga tctggccgcg cgctcgggcc aggtcgtcaa
      841 tggacaggta atagcgctgc accgacatga aatctcctcg aacggcctgc aagtctagca
      901 gagccgcgcg cgtgcaggtc cgtgactttc gcccctgccc gggcgcggcg gcggacgcta
      961 gaatccgcca ccttttccgt ccgctggaag atgcccatgt cccaacgccg cctgcagctt
     1021 gccgccactc tcgtgctttg cgcgtcgacg ccattcgcgt tcgccggtac ccaattgctg
     1081 cgctttcccg acgtctgcgg cgatcgcatc gcgttcacct acgcgggcga cctgtggacc
     1141 gcttcgacgc agggcggcac cgcgacgcgt ctgaccgcgg gcccgggcct ggaacaatcg
     1201 gcgcgcttct cgccggactg caagacgatc gcattcaccg gcgaatacgg tggcgacgac
     1261 caggtctaca cgatcccggc ctacggtggc gaaccgaagc agctgacgtt ctatccgtcc
     1321 aagggtccgc tgccgcagcg ctggggattc gacagccagg tctacggctg gacgccg
//
LOCUS       DASRVO010000841         1076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_336996, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000841 DASRVO010000000
VERSION     DASRVO010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336996"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VFF05_10385"
     CDS             complement(<1..145)
                     /locus_tag="VFF05_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridylate synthase"
                     /protein_id="HZW52261.1"
                     /translation="MLIALNKPFNVLCQFTDRNEPKRRTLAEFVNAVDVYPAGRLDYD
                     SEGL"
     gene            complement(216..>1076)
                     /locus_tag="VFF05_10390"
     CDS             complement(216..>1076)
                     /locus_tag="VFF05_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZW52262.1"
                     /translation="PAVPPPKVADQPANPPPPQSAQQMFDAKIDAVLAGKWREAANST
                     RDQYRHPKQTLEFFGLKSGMNLIEITPAAGWYAQVLAPLMEDDGSYTAAITIPKKPAG
                     EAAQDKTALEKMFAADPAQYGKAKVVQYDPKAPNLGVPGSADMVVTFRNVHNWVMGDY
                     APGMFKAFFDVLKPGGVLGVTDHRAAPGADLEKIKRFGYLPEDYVIKLATDAGFKLDG
                     KSEINANPKDTKDYPKGVWTLPPTLTLGDKDKEKYLAIGESDRMTLRFVKPQGDQIFS
                     APADSKQKTN"
BASE COUNT          166 a          333 c          344 g          233 t
ORIGIN      
        1 gcaggccttc ggaatcgtaa tccagccgac ccgctggata gacgtcgaca gcattcacga
       61 attcggccag cgtgcgccgc ttcggttcgt tgcggtcggt gaactggcac agcacgttga
      121 acggtttgtt gagcgcgatc aacaccgggc aatcccgcag atgcgaacgg ccgcgcgtgg
      181 cggccgttcg gaatgcagca gcgtcttgcg ccagctcaat tcgtcttctg cttgctgtcc
      241 gccggagcgg aaaagatctg atcgccctgc ggcttgacga agcgcagtgt catgcggtcg
      301 gattcgccga tcgcgagata tttttccttg tccttgtcgc cgagcgtcaa ggtcggcggc
      361 agcgtccaca cgcctttcgg ataatccttg gtgtccttgg gattggcgtt gatctcgctc
      421 ttgccgtcga gcttgaagcc ggcgtcggtg gcgagtttga tcacgtaatc ctcgggcagg
      481 tagccgaagc gcttgatctt ttcaagatcc gcgccgggcg cagcacggtg atcggtcacg
      541 ccgagcacgc cgccgggctt gagcacgtcg aagaaggcct tgaacatgcc cggcgcatag
      601 tcgcccatga cccagttgtg cacgttgcgg aacgtcacga ccatgtccgc tgaacccggc
      661 acgccgaggt tcggcgcctt cggatcgtat tgcacgacct tggccttgcc atactgcgcc
      721 gggtcggcgg cgaacatttt ctcgagcgcc gtcttgtcct gcgcagcctc gcctgcgggt
      781 tttttcggaa tcgtaatggc cgcggtgtag ctgccgtcgt cctccatcag cggcgcgaga
      841 acttgcgcat accagccggc ggccggcgtg atctcgatca ggttcatgcc ggacttgagg
      901 ccgaagaatt ccagcgtctg tttcggatgc cggtactggt cgcgcgtcga gttcgccgcc
      961 tcgcgccact tgcccgcgag taccgcatcg atctttgcat cgaacatctg ctgcgcgctt
     1021 tgcggcggcg gcggattggc cggctggtcc gctaccttcg gcggcggtac ggcggg
//
LOCUS       DASRVO010000842         1126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_285605, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000842 DASRVO010000000
VERSION     DASRVO010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285605"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFF05_10395"
     CDS             <1..312
                     /locus_tag="VFF05_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017915820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52263.1"
                     /translation="WAGCPVLTLPGQTFASRVAGSLNHHHGMQQMNATSDQDYVDKAV
                     QLARFPPMLAALRSRVEDARRNSGLFDMAGYARDFTAALTLMFQRSERGLAPEDLEVH
                     A"
     gene            309..830
                     /gene="moaB"
                     /locus_tag="VFF05_10400"
     CDS             309..830
                     /gene="moaB"
                     /locus_tag="VFF05_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011346528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HZW52264.1"
                     /translation="MSASDHFISQSLCVLTVSDTRTAANDASGDYLCASLTEAGHRLH
                     ERRLARDDRYLVRAIVAAWIADPAVDGILVTGGTGFTGRDSTPEAIAPLLDKEMTGFG
                     EMFRALSYEEIGTSTLQSRAFAGLANATFVFCLPGSTSACRTAWEKLLCAQLDSRTKP
                     CNLAGLKPRLREA"
     gene            complement(837..1103)
                     /locus_tag="VFF05_10405"
     CDS             complement(837..1103)
                     /locus_tag="VFF05_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017112879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZW52265.1"
                     /translation="MPCAKFNVRGRVQGVAFRAHARELARELGLTGHAKNLPDGSVEV
                     LACGDAAALDELESWLRRGPPAARVDAVTRTAGEMPPPASFTTA"
BASE COUNT          178 a          398 c          358 g          192 t
ORIGIN      
        1 tgggcgggct gcccggtgct taccctgccc ggccagacgt ttgcctcgcg cgtggccggc
       61 agcctcaacc accatcatgg catgcagcag atgaacgcga catcggacca ggattacgtc
      121 gacaaggccg tgcagctggc gcgctttccg ccgatgctcg cggcgttgcg cagccgcgtc
      181 gaggacgcac gccgcaattc cggcctgttc gacatggcgg gctatgcgcg cgacttcacg
      241 gccgcgctca cgctgatgtt ccagcgcagc gagcgcggtc tcgctcccga ggatctcgag
      301 gtacatgcat gagcgcgagc gaccatttca tttcccagag cctgtgcgtg ctgaccgtct
      361 cggacacgcg caccgcggcg aacgacgctt ccggcgatta tctgtgtgcg tcgctgaccg
      421 aggccggcca ccgcctgcac gaacgccgcc tcgcgcgcga cgatcgctat ctggtgcgcg
      481 ccatcgtcgc cgcatggatt gccgatcctg cggtggacgg tattctcgtc accggcggta
      541 ccggcttcac cggtcgcgac tcgacgcccg aagcgatcgc accattgctg gacaaggaaa
      601 tgaccggttt cggcgagatg ttccgcgccc tcagctacga ggaaatcggc acctccacgc
      661 tacagtcgcg cgcgttcgcc ggactcgcca acgcgacgtt cgtgttctgc ctgccgggct
      721 cgacctcggc ctgccgcacg gcttgggaaa agttgctttg tgcacaattg gattcgcgca
      781 cgaagccgtg caatctggcc ggcctgaaac cccgcctgcg cgaggcctga gaaacgctac
      841 gccgtcgtga acgatgccgg tggcggcatt tcaccagccg tgcgtgttac cgcatccacc
      901 cgcgccgcgg gcgggccgcg ccgcagccag gattcgagtt cgtccaacgc cgcggcatcg
      961 ccgcaggcca gcacctcgac gctgccatcg ggcaggttct tcgcatggcc ggtcaggcca
     1021 agctcccgtg ccagttcgcg cgcatgcgcg cgaaacgcca cgccctgcac gcggccgcgc
     1081 acgttgaatt tcgcgcaagg cacgagtcag cggattccgg tggcgt
//
LOCUS       DASRVO010000843         5078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_207441, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000843 DASRVO010000000
VERSION     DASRVO010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207441"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..918
                     /gene="hemC"
                     /locus_tag="VFF05_10410"
     CDS             1..918
                     /gene="hemC"
                     /locus_tag="VFF05_10410"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZW52266.1"
                     /translation="MKDVLRIATRKSALALWQAEHVAARLRAAHPHLGVELVPMTTRG
                     DKILDRPLADIGGKGLFLKELEVALEQREADIAVHSFKDVPMRLDDDFAIGAVLERAD
                     AADAFVAVNFVHLDDLPRGARVGTSSLRRQAQLRARRPDLELLDLRGNVNTRLAKLDN
                     GDYDAIVLACAGLERLGLAARIRSRLEPPHWLPAAAQGAIAVELRAGDARTLEVLASL
                     HDEATARCVAAERAMTRRLEGSCQVPIAAFCIETEIGLHLSGLVGDAASGKLIRAEDD
                     GQSEAPEALGARVARALLAQGAGELLGHK"
     gene            complement(964..1743)
                     /locus_tag="VFF05_10415"
     CDS             complement(964..1743)
                     /locus_tag="VFF05_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HZW52267.1"
                     /translation="MRTTLLASAVLLALPLAALADAPPPPPQGWSGSGEAGLSVASGN
                     TKSQNLNAKLDIKFNDDQWTDDFYLTALRNKANTPAGYDLTANRYEGGASLGYKLDER
                     SYIVGAARYEHDEFSSYDYQYIVSIGYGYQILKNASDELAVEVGPGYKVAQPASYILL
                     PVDPLSPPVKVTPDSENNLVARGKLAYKHNFSDTVSFVDTFVVEAGSGNKFFQNDAGL
                     AVKMNSKLALKVGYQIRHNSDVEPGFKKTDGLFTTNLVYNF"
     gene            complement(1854..2804)
                     /locus_tag="VFF05_10420"
     CDS             complement(1854..2804)
                     /locus_tag="VFF05_10420"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HZW52268.1"
                     /translation="MNPAFSVIFFTVVSGTGYGLLFLLGIMLAFDPSSLGRNEALLAL
                     GVGATFVVAGLLSSTLHLGQPQRAWRALSQWRSSWLSREGVASLASFVPIFALAWCLW
                     RSADEALLRGLGALLAVLAIITVACTARIYTCLKTIPAWHNAYVLPGYLLLGALGGAC
                     WSWLIGEWIGGARAQPTLIVTLAIASSALKFAYWRFIDTSPPTSTPESATGLGRFGRV
                     RSVEAPHTEENYLTQEMGFVLARKHAGRLRAICLGLIGAIPVLLAMAMLFSPLAANAI
                     FACIAALSVTAGLFVERWLFFAEAKHVVMLYYGGAQTQRA"
     gene            complement(2801..3553)
                     /locus_tag="VFF05_10425"
     CDS             complement(2801..3553)
                     /locus_tag="VFF05_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZW52269.1"
                     /translation="MTMLPPPTRKKLGLVIDLDICVGCHACAVACKEWNDGGEFGPLP
                     DENPYGAEPLGVWFNRVHTYEIEPKEENKPAMTVHFPRSCLHCETPDCVTVCPTGASY
                     KRAEDGIVLVDEDKCIGCKLCSWACPYGAREYSEVRGVMQKCTLCVDRIYNESFDEVD
                     RQPACVMACPTRARHFGDLGDADSTVSNLVAERGGYDLMPEIGYQPVNKYLPPRQRRD
                     GCGSAPTKTAQDEKVDFSTSPLLRWMDKVLSR"
     gene            complement(3564..>5078)
                     /locus_tag="VFF05_10430"
     CDS             complement(3564..>5078)
                     /locus_tag="VFF05_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007082538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZW52270.1"
                     /translation="IQRPWLLTLSILVIVCFLGAFVKNTALVATFLPVTLSVCARAKT
                     SPARVLIPMSYAAQMGGVCTLLGTSSNLLTNSLAVQHGMPEFGVFDFTKLGVVLAIAG
                     IVYLMLIGRWLLPRHIDTDHSENLELGKFVTELRVLADSPLIGESVAAAKLGERFGVY
                     VLELLRGDDHLWAPREQELREGDVLLARGDWQRLEEFKQKLKLEIAPVLDLPEPAPAG
                     RRVLIEALVAPGARVVGDTVENIDFRWTYNAAVLAVQRRSRILREQLKFTRLEVGDIL
                     LLLVDEAALRQLRRESGLVVLSERGDTRASRRKAPLAIAILATVVIVAGLGILPIVVS
                     ALAGCAAMALTGCFSFEDVYETMDWKVIALLGAILPLGIALDKTGLAHELVDAGMHLV
                     GTHGPLAVLLMVYLLTALLTEMMGHNPSVVLMVPIAISIAHVLQVSPTPFVICVAFAA
                     ATSFATPVGYPTNTMVYAAGSYRFADFMKVGIPLIAMFCALSMWLIPQFWPFHP"
BASE COUNT          937 a         1610 c         1687 g          844 t
ORIGIN      
        1 atgaaggacg tgctgcgcat cgccacgcgc aagagcgcgc tcgcactgtg gcaggccgag
       61 catgtggccg cgcgcctgcg cgccgcgcat ccgcatctgg gcgtcgagct cgtgccgatg
      121 acgacgcgcg gggacaagat cctggatcgt ccgctcgccg acatcggcgg caaggggctg
      181 tttctcaagg aactcgaagt cgcgctagag cagcgcgagg ccgacatcgc cgtgcattcg
      241 ttcaaggacg taccaatgcg tctggacgat gacttcgcga tcggtgccgt gctcgagcgt
      301 gcggatgcgg ccgacgcctt cgttgccgtg aactttgtgc atctggacga tttgccgcgt
      361 ggcgcgcgcg ttggtacctc gtcgctgcgc cggcaagcgc aactgcgtgc acggcgtccg
      421 gacctcgaac tgctcgatct gcgcggcaac gtcaacacgc gcctggccaa gctcgacaac
      481 ggcgattacg acgccatcgt tctcgcctgc gcgggactgg agcgcctggg cctcgccgcg
      541 cgtatccgta gccggctcga accgccgcac tggttgccgg ccgcggcgca gggcgcgatc
      601 gccgtcgaac tgcgtgccgg cgacgcacgc acgctggagg tgctggcgtc gctgcacgac
      661 gaggcaacgg cgcgctgcgt cgctgccgag cgcgcgatga cgcgccgtct ggaaggcagc
      721 tgccaggtgc cgatcgcggc gttctgcatc gaaactgaaa tcggcttgca tctttccgga
      781 ctggtcggcg acgccgccag cggcaaactc atccgcgccg aggacgatgg ccaatccgaa
      841 gcgccggaag cgctcggcgc gcgcgtcgcg cgcgcgctgc tcgcgcaagg cgcgggggaa
      901 ttgctggggc acaaatgaca acgggcccgc gtggggcccg ttgcgaattg gacgagcaaa
      961 cgttcagaag ttgtagacga ggttcgtcgt gaaaagccca tccgtcttct tgaagcccgg
     1021 ttccacgtcc gaattgtggc ggatctggta gccgaccttg agcgccagct tgctgttcat
     1081 cttcaccgcg agtccagcat cgttctggaa gaacttgttg ccgctgccgg cttcgaccac
     1141 gaacgtgtcg acgaacgaca ccgtgtcgct gaaattgtgc ttgtaggcga gcttgccgcg
     1201 cgcgacgaga ttgttctcgg aatccggcgt gaccttgacc ggcggcgaga gcggatcgac
     1261 cggcaacagg atgtacgaag ccggctgcgc gaccttgtag cccgggccga cttccaccgc
     1321 aagttcatcg ctcgcgttct tcaggatctg gtagccgtag ccgatcgaga cgatgtactg
     1381 gtagtcgtac gacgagaatt cgtcgtgttc gtaacgtgcc gcaccgacga tgtagctgcg
     1441 ctcgtcgagc ttgtagccga gcgaagcgcc gccttcgtag cggttcgcgg tcaggtcgta
     1501 gccggccggc gtattggcct tgttgcgcag cgcggtcagg tagaaatcgt cggtccactg
     1561 gtcgtcgttg aacttgatgt cgagcttggc gttcaggttc tgcgacttgg tgttgccgct
     1621 ggcgacggac aggccggcct cgccgctgcc ggaccagccc tgcggcggcg gtggcggcgc
     1681 atcggcgagc gcggccagcg gcagcgcgag caagacggcg gatgcgagca atgtcgttct
     1741 catgggtaat tccttgttct tggggacgcg ccgcgtgcaa ggcggcgggc gcgcattgta
     1801 aacgcgcgtc gatgcaaaca agtggacttg tgcgcaaaac gtacaagcgc cgttcaggcg
     1861 cgttgtgtct gtgcgccacc gtagtacagc atcacgacgt gtttcgcttc ggcgaaaaac
     1921 agccagcgct cgacgaacag gccggcggtg acggaaagcg ccgcgatgca cgcaaagatc
     1981 gcgttcgccg caagcggcga gaacagcatg gccattgcga gcaaaaccgg gattgcgccg
     2041 atcaggccaa ggcaaatcgc gcgcagacgc cccgcgtgct tgcgcgcgag aacgaagccc
     2101 atttcctgcg tcaggtaatt ttcttccgtg tgcggcgcct cgacgctgcg cacgcggccg
     2161 aagcggccaa gtccggtggc cgattccggc gtcgaggtcg gcggcgacgt gtcgatgaat
     2221 cgccagtacg cgaacttgag cgcgctgctg gcgatggcaa gtgtgactat caatgtcggt
     2281 tgcgcgcgcg cgccgccgat ccattcgccg atcagccagg accagcatgc cccgccgagc
     2341 gctcccaaca acagatagcc cggcaacacg taagcgttgt gccaggccgg tatcgttttc
     2401 aggcaggtgt agattcttgc cgtgcacgcg acagtaatga tggcgagcac cgcgagcaag
     2461 gcaccgagtc cgcgcaacaa cgcttcatct gcgctgcgcc acaggcacca tgccagcgcg
     2521 aaaatcggta cgaagctcgc cagcgaggcc acgccctcgc gcgacaacca cgacgaacgc
     2581 cactgcgaaa gcgcgcgcca ggcgcgctgc ggctggccga ggtgcagggt cgaggaaagc
     2641 agccccgcga cgacgaaagt cgcgccgacg ccgagtgcga gcaacgcctc gttgcgaccg
     2701 agactcgacg gatcgaacgc cagcatgatt ccgagcagga acagcaatcc atagccggtg
     2761 ccggacacga cagtaaagaa gatgacggag aaggcggggt tcacctcgac aacaccttgt
     2821 ccatccagcg caacaatggc gatgtggaaa aatccacttt ttcatcctgc gcggttttgg
     2881 ttggcgcgga gccacaaccg tcgcggcgct ggcgcggcgg caggtatttg ttcaccggtt
     2941 ggtagccgat ctcgggcatc agatcgtagc cgccgcgttc ggcgacgagg ttcgacaccg
     3001 tcgaatccgc gtcgccgagg tcgccgaaat gccgcgcgcg cgtggggcag gccatcacgc
     3061 aggccggctg gcggtcgact tcgtcgaagg attcgttgta gatgcggtcc acgcacagcg
     3121 tacatttctg catcacgccg cggacttcgc tgtattcgcg cgctccgtaa gggcaggccc
     3181 acgagcacag cttgcagccg atgcacttgt cctcgtccac gagcacgatg ccgtcctcgg
     3241 cgcgcttgta gctcgcgccg gtcggacaca cggtgacgca atccggcgtt tcgcaatgca
     3301 ggcaggagcg cggaaagtgc accgtcattg ccggcttgtt ttcttccttt ggctcgattt
     3361 cgtacgtgtg cacgcgattg aaccagacgc ccagcggctc cgcgccatac ggattctcgt
     3421 ccggcagcgg gccgaactca ccgccgtcgt tccattcctt gcacgcgacc gcgcaggcgt
     3481 ggcagccgac gcagatgtcg aggtcgatga ccaggccgag tttcttgcgc gttggcggcg
     3541 gcaacatcgt catggcgctt cgctcagggg tggaacggcc agaactgcgg gatcagccac
     3601 atcgacagcg cgcagaacat cgcgatcagc gggatgccga ctttcatgaa atccgcgaaa
     3661 cgatagctgc cggccgcgta caccatcgtg ttggtcggat agccgaccgg cgtggcgaac
     3721 gaagtggcgg cggcgaacgc gacgcagatc acgaacggcg tcgggctcac ctgcaggacg
     3781 tgggcgatgg aaatcgcgat cggcaccatc agcaccacgg acggattgtg gcccatcatc
     3841 tcggtgagca gggcggtgag caggtagacc atgagcagga cggcgagcgg accgtgcgtg
     3901 ccgacgaggt gcatgccggc gtcgacaagc tcgtgcgcga gtccggtctt gtccagcgcg
     3961 atgccgagcg gcaggatcgc gccgagcagg gcgatgacct tccagtccat ggtttcgtac
     4021 acgtcctcga aggaaaaaca gccggtcagc gccatcgccg cgcagccggc gagcgcgctg
     4081 acgacgatcg gcaggatgcc gagtccggcg acaatgacca cggtcgcgag aatggctatc
     4141 gcgagcggcg ccttgcgccg cgaggcgcgc gtgtcgccgc gctcgctcag cacgacgagg
     4201 ccggattcgc ggcgcaactg gcgcagtgcc gcctcgtcga ccagcagcag caggatgtcg
     4261 ccgacttcca gacgggtgaa cttcaattgt tcgcgcagga tgcggctgcg gcgttgcacc
     4321 gcaagcacgg cggcgttgta ggtccagcga aagtcgatgt tctcgaccgt atcgccgacc
     4381 acccgcgccc ccggcgcgac cagtgcctcg atcagcacgc ggcgtccggc tggcgcgggt
     4441 tcgggcaggt caagcaccgg cgcgatttcg agtttcagct tctgcttgaa ttcctccagg
     4501 cgctgccagt cgccgcgcgc gagcagcaca tcgccttcgc gcaattcctg ttcgcgcggc
     4561 gcccacaggt ggtcgtcgcc acgcaacagt tccagcacat acacgccgaa acgctcgccg
     4621 agcttagccg cggcgaccga ttcgccgatc agaggcgaat cggcgagcac gcgcagctcg
     4681 gtcacgaact tgccgagttc gaggttttcg gaatgatcgg tatcgatgtg gcgcggcagc
     4741 agccagcgcc cgatcagcat caagtacacg atgccggcga tggccagcac gacgccgagc
     4801 ttggtgaaat cgaagacgcc gaattccggc atgccgtgct gcacggccaa actgttcgtc
     4861 aacaggttcg atgaagtgcc caacaacgtg cacacgccgc ccatctgcgc cgcgtaggac
     4921 atcgggatca gcacgcgcgc cggcgatgtt tttgcgcgcg cgcagacgct cagcgtcacc
     4981 ggcaagaacg tcgcgaccag cgccgtgttc ttcacgaacg cgccaaggaa gcagacgatg
     5041 acgaggatcg aaagcgtcag cagccacggc cgctggat
//
LOCUS       DASRVO010000844         4326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_24985, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000844 DASRVO010000000
VERSION     DASRVO010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24985"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFF05_10435"
     CDS             complement(<1..406)
                     /locus_tag="VFF05_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZW52271.1"
                     /translation="MRLGVLDVEHTEKDGNLAGLKVMVIDDSKTIRRTAETLLKKEGC
                     EVVTATDGFEALAKISDQQPNIIFVDIMMPRLDGYQTCALIKKNPKFRVTPVVMLSSK
                     DGLFDRARGRIAGSDEYLAKPFTRESLLEAVGR"
     gene            730..1683
                     /gene="gshB"
                     /locus_tag="VFF05_10440"
     CDS             730..1683
                     /gene="gshB"
                     /locus_tag="VFF05_10440"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HZW52272.1"
                     /translation="MSLRVAMLMDPIESIHIEKDSSFAMLLEAQRRGHELLYLTQGSL
                     AIRDAAPWAQARALRVRDHARGWFELDAPHWCDLRRVDVILARKDPPVDAQFVYDTMV
                     LELAQAGGALVVNDPRSLRDANEKLFALHFPQCIPTTLVARDAGEIKRFVAEQGEVVI
                     KPLDGMGGRSIFRSAHGDPNLNVILETLTDNGRAFAMAQRYLEEIRDGDKRILLIDGE
                     AVPYALARVPQGDEFRGNLAAGGKGVGMPLTERERWIANQVGPELRARGMLFVGLDVI
                     GGFLTEVNVTSPTCIRELDAQFGLNIAGQLFDAIEAKCRPQ"
     gene            1680..2543
                     /locus_tag="VFF05_10445"
     CDS             1680..2543
                     /locus_tag="VFF05_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZW52273.1"
                     /translation="MSAAAPAIGSRDRLSVTFLFSLIFHGVLALGLTFAYEKPAPSLP
                     ALDVILVQSASGQKPTHADFLAQANNTGGGDSERALRPSALVSSPVNKPDPGVAPRPL
                     RASMPRPSPPTERELLTSPQSDFSVRSEHETPDQPPLPQPTLHDMLQTKLEMAKLATE
                     IQRESQAYAKRPHKKYISANTKEYAYAAYMAAWVARVERIGNLNYPDEARREHVHGDL
                     VLTVALNRSGAIKRVDVIHSSGHKILDDAAIRIVRLAAPFPPIPKDEGIDELYITRTW
                     QFLPGDILRNR"
     gene            2583..3146
                     /locus_tag="VFF05_10450"
     CDS             2583..3146
                     /locus_tag="VFF05_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZW52274.1"
                     /translation="MQSTSSLANQFLIAMPGTRDPLFKRSVAFVCQHGEDGAMGLLVN
                     RLSEYRLGDVLAQMNIKSSLSRISEAPVLIGGPVQPERGFVLHAPPGDWESSYKISER
                     LCVTTSRDVLVAMAEGNGPEQAIVALGYAGWSPGQIENELKENAWLTCAADEAILFSA
                     PLETRWEAAANLVGVNIASLTDYAGHA"
     gene            3130..3573
                     /gene="ruvX"
                     /locus_tag="VFF05_10455"
     CDS             3130..3573
                     /gene="ruvX"
                     /locus_tag="VFF05_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZW52275.1"
                     /translation="MPDTPEAVSTVLGFDYGARRIGVAVGNALTASARALEVIANGAQ
                     GPDWPRVDALMREWRPQILLVGLPLTMEGEEQKNSAAARAFAAQIGARCKLPTRLVDE
                     RLSSHAAAQRFAQRRAAGQARRKHAAALDAVAAEIIIETWFGQEV"
     gene            3576..>4326
                     /locus_tag="VFF05_10460"
     CDS             3576..>4326
                     /locus_tag="VFF05_10460"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HZW52276.1"
                     /translation="MGNASATQLDANGRPRHLLTLEGLPRATIVALLDRAEALRAESR
                     GGTRPLDLLRGRTVINLFFEPSTRTRTSFDLAAQRLSATVINFDIASSSTLKGETLLD
                     TVHTLEAMHADAFVVRHKESGTPEFIAANLRSNAAVLNAGDGNRAHPTQGLLDALTLR
                     GHVADFSKLRVAICGDIRHSRVARSDVHALAALGVRDIRLCAPQELAPDAGEFPQCRI
                     FHDFDEAIAGVDVAIMLRLQKERMQAAAIPSE"
BASE COUNT          785 a         1496 c         1334 g          711 t
ORIGIN      
        1 gccgacccac ggcttcaagc aggctttctc tggtaaacgg tttggccaaa tattcgtccg
       61 accccgcgat cctgccgcga gcacggtcga acaggccatc cttgctggat agcatgacga
      121 cgggcgtgac cctgaatttg gggtttttct tgatcagcgc acaggtctgg tagccgtcca
      181 ggcgcggcat catgatgtcg acgaaaatga tgttcggctg ctggtcggaa atcttcgcca
      241 acgcttcgaa accgtcggtc gcggtgacca cctcgcagcc ttctttcttg agcagcgttt
      301 cggccgtgcg gcggatcgtt ttcgagtcgt cgatgaccat gaccttcagg ccggccagat
      361 tgccatcttt ctcagtgtgt tccacgtcca aaacccccaa ccgcatggag tgcatgatgt
      421 gtacgtcgac tcggcggcgg aatccggctg ctctgccggg ccccgcgcgg cgacgcgcgc
      481 gcctgttccg agcctgttta accaatactt agcaaatatg tcaagtaaat ccgctaaccc
      541 gtcattattg ctcgggaaaa acttgccgcc gcggcgacct tcgcctgcgg atgcgagcct
      601 ttggcaggca cgccgcgatg ctagtatagc cccgtatcca ccgttgcgcg ttcctgcaat
      661 gccgcaaaaa acaacacaag aaaccagcac atcccgtgcc aacgaccatg atccgcggag
      721 cgcaacgcca tgagcctgcg cgtggccatg ctgatggacc cgatcgagtc catccatatc
      781 gagaaggatt ccagcttcgc gatgctgctc gaagcgcagc gccgtggcca cgaattgctc
      841 tacctgacgc aaggctcgct ggccatccgc gacgcggcgc cctgggcgca ggcacgggcc
      901 ttgcgcgtac gcgaccacgc gcgcggctgg ttcgaactcg acgcgccgca ctggtgcgac
      961 ttgcgcaggg tcgacgtgat cctcgcgcgc aaggatccgc cggttgacgc gcaattcgtc
     1021 tacgacacga tggtgctgga actcgcccaa gccggcggcg cgctcgtggt caacgatccg
     1081 cgctccctgc gcgacgccaa cgaaaaactt ttcgcgctgc atttcccaca atgcattccg
     1141 acgaccctgg tcgcgcgcga cgccggcgag atcaagcgct tcgtcgccga acaaggcgag
     1201 gtcgtgatca agccgctcga cggcatgggc ggacgctcga tcttccgcag cgcgcacggc
     1261 gatccgaatc tcaacgtgat cctggaaacc ctcaccgaca atggccgcgc gttcgcgatg
     1321 gcgcagcgct atctggaaga aatccgcgac ggcgacaagc gcatcctgct gatcgacggc
     1381 gaagcggtgc cgtacgcgct ggcgcgcgtg ccgcagggcg acgaattccg cggcaacctg
     1441 gccgccggtg gcaagggcgt gggcatgccg ttgaccgagc gcgagcgctg gatcgccaac
     1501 caggtcgggc ctgaactgcg cgcgcgcggc atgctcttcg tcggcctgga cgtgatcggc
     1561 ggattcctca ccgaagtcaa tgtcacctcg ccaacctgca tccgcgaact ggatgcgcaa
     1621 ttcggcctga acatcgccgg gcaattgttt gacgcgatcg aggcgaaatg caggccgcaa
     1681 tgagcgccgc tgcaccggcc atcggcagcc gcgaccgcct cagcgtgacg ttcctgttct
     1741 cgctgatctt ccacggcgtg ctcgcgctcg gactcacctt cgcctacgaa aaaccggcgc
     1801 cgagcctgcc cgcgctcgac gtcatcctgg tgcagtcggc ctcgggccag aaaccgaccc
     1861 atgcggactt cctcgcccag gccaacaaca ccggtggcgg cgacagcgaa cgcgccttgc
     1921 gtcccagcgc gctcgtctcc agccccgtga acaagcccga tcccggcgtt gcgccgcggc
     1981 cgctgcgcgc gtccatgccg cggccctcgc cgccgaccga gcgcgaactg ctcacttcgc
     2041 cgcagtcgga tttttccgtg cgcagcgagc acgagacccc ggatcagccg ccgctgccgc
     2101 agccgaccct gcacgacatg ctgcagacga aactggaaat ggccaagctc gccaccgaaa
     2161 tccagcgcga aagccaggcc tacgccaagc gtccgcacaa gaagtacatc tcggccaaca
     2221 ccaaggaata cgcctacgcc gcctacatgg ccgcttgggt cgcgcgcgtg gagcgcatcg
     2281 gcaacctcaa ctatccggac gaggcgcgcc gcgaacacgt gcacggcgac ctcgtgctca
     2341 ccgtcgcgct caaccgcagc ggcgcgatca agcgcgtcga cgtgatccat tccagcgggc
     2401 acaagatcct cgacgacgcc gcgatccgca tcgtgcgcct ggccgcaccg tttccgccga
     2461 tccccaagga cgaaggcatc gacgagctgt acatcacccg cacctggcag tttttgccgg
     2521 gcgatatcct gagaaatcgt tgacgaacct ctgactagcc tgctgcgcgg tacaatccac
     2581 gcatgcaatc cacctcctcg cttgccaacc agttcctgat cgcgatgccg ggcacgcgcg
     2641 acccgctgtt caagcgcagc gtggcgtttg tctgccagca cggcgaggac ggcgcgatgg
     2701 gcctgctcgt caaccgcctg tccgaatacc gcctcggcga cgtgctcgcg cagatgaaca
     2761 tcaagtcgtc gctcagccgt atcagcgaag cgccggtgct gatcggcggg ccggtgcaac
     2821 ccgagcgcgg cttcgtgctg catgcgccgc cgggcgactg ggaatcctcg tacaagattt
     2881 ccgaacgcct gtgcgtgacc acctcgcgcg acgtgctcgt ggcgatggcc gaaggcaacg
     2941 gtccggagca agccatcgtc gcgctcggtt atgccggctg gagccccggg cagatcgaga
     3001 acgagctgaa ggaaaatgcc tggctgacct gcgcggcgga cgaggcgatc ctgttttccg
     3061 cgccgctgga gacgcgttgg gaagcggcgg cgaacctggt cggcgtcaac atcgccagtc
     3121 tgaccgatta tgccggacac gcctgaagcg gtttcgaccg tgctcggctt cgactacggc
     3181 gcgcgtcgca tcggcgtggc ggtcggcaac gcgctgaccg catcggcgcg tgcgctcgag
     3241 gtgatcgcga atggcgcgca ggggccggac tggccgcgcg tggatgcgct catgcgcgag
     3301 tggcgaccgc agatcctgct ggtcggcctg ccgttgacga tggaaggcga ggaacagaaa
     3361 aacagcgcag cggcacgcgc attcgccgcg cagatcggcg cacgctgcaa gctgccgaca
     3421 cgactcgtgg acgaacgcct gagttcgcac gcagccgcgc agcgttttgc gcaacgccgc
     3481 gctgccggac aggccaggcg caagcacgcg gcggcgctgg atgcggtcgc ggccgaaatc
     3541 atcatcgaaa cctggttcgg tcaagaagta tgaaaatggg caacgcatcc gccacccaac
     3601 tcgacgccaa cggccgcccg cgccatctgc tcacgctcga gggcttgccg cgcgcgacga
     3661 tcgttgccct gctcgaccgc gcggaagcct tgcgcgcgga aagtcgcggc ggcacacggc
     3721 cgctggacct gctgcgcggc cgcaccgtca tcaacttgtt cttcgagccg tccacgcgca
     3781 cgcgcacctc gttcgatctg gccgcgcagc gcctgagcgc cacggtgatc aatttcgaca
     3841 tcgccagctc gtccacgctc aagggcgaaa ccttgctcga taccgtgcac acgctcgaag
     3901 ccatgcatgc cgacgcattc gtcgtgcgcc acaaggaatc cggcacgccg gaattcatcg
     3961 ccgcgaatct gcgctccaat gccgcggtgc tcaacgccgg cgacggcaac cgggcgcatc
     4021 cgacgcaagg cctgctcgat gcgctgactt tgcgcggcca cgtcgcggat ttctcgaaac
     4081 tgcgcgtggc gatctgcggc gacattcgcc actcgcgcgt ggcgcgctcg gacgtgcacg
     4141 cgctcgccgc gcttggcgtg cgcgacatcc gcctgtgcgc gccgcaggaa ctggccccgg
     4201 acgcgggcga atttccacaa tgccgcattt tccacgattt cgacgaagcc atcgccggcg
     4261 tggacgttgc catcatgctg cgcctgcaaa aggagcgcat gcaggccgcg gcgattccct
     4321 ccgagg
//
LOCUS       DASRVO010000845         1521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_285686, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000845 DASRVO010000000
VERSION     DASRVO010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285686"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VFF05_10465"
     CDS             complement(<1..104)
                     /locus_tag="VFF05_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZW52277.1"
                     /translation="MSAGTLFIVAAPSGAGKTSLTRALLEGDPNLTLSV"
     gene            complement(101..979)
                     /locus_tag="VFF05_10470"
     CDS             complement(101..979)
                     /locus_tag="VFF05_10470"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZW52278.1"
                     /translation="MIRSMTAFASAETSTPWGALAFELRSVNHRYLELNPRLPDELRA
                     IEPALRERVATKLTRGKVDVTLRFRPNDADASQLRVNDAMLERLAQLAKSFEERFPDL
                     DTDFTSVLQWPGVLVQNDIDQEGLREAALKLLDVALADMLATRAREGERLGVFLRERL
                     DGIEKTVADVRGWLPHIREALRAKLQSRLGELKPAAGLAQLEPGRIEQELVLQLSRID
                     VDEELDRLSAHIAEARRVLALPDAVGRRMDFLMQEFNREANTLGSKSVDSRTTQASVE
                     LKVLIEQMREQVQNIE"
     gene            1046..>1521
                     /gene="rph"
                     /locus_tag="VFF05_10475"
     CDS             1046..>1521
                     /gene="rph"
                     /locus_tag="VFF05_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZW52279.1"
                     /translation="MAAALISTGSRPSGRAPDQLRTVVIERRYTRHAEGSVLVAFGDT
                     RVLCTASIEDKAPPFLRGKGEGWITAEYGMLPRATGERTAREAARGKQGGRTLEIQRL
                     IGRALRACVDRNALGERTITLDCDVLQADGGTRTASITGAYVALVDAINWLKARDK"
BASE COUNT          247 a          501 c          505 g          268 t
ORIGIN      
        1 accgacaagg tcaggttcgg atcgccttcg agcagcgccc gcgtcaggct ggtcttgccc
       61 gcacccgacg gtgccgcgac gatgaagaga gtgcccgcgc tcattcgatg ttctgcacct
      121 gctcgcgcat ctgttcgatc agcaccttca gttccacgct ggcctgcgtg gtacgcgagt
      181 cgacggactt ggaaccgagc gtgttcgcct cgcggttgaa ttcctgcatc aggaaatcca
      241 tgcgccggcc gaccgcgtcg ggcagtgcga gtacgcggcg tgcctcggca atgtgcgcgc
      301 tcaggcggtc gagctcctcg tccacgtcga tgcgcgaaag ttgcagcacg agttcctgct
      361 cgatccgacc cggttcgagt tgcgccagtc ccgccgccgg cttgagttcg ccgaggcgcg
      421 attgcagttt ggcgcgcaac gcttcacgga tgtgcggcag ccagccgcgc acgtcggcga
      481 cggtcttttc gatcccgtcc aggcgctcgc gcaggaacac gccgaggcgt tcgccctcgc
      541 gagcgcgcgt ggcgagcatg tcggccaacg caacgtccag cagtttcagc gcggcctcgc
      601 gcaagccttc ctgatcgatg tcgttctgca ccagcacgcc gggccattga agcacgctgg
      661 tgaagtcggt atcgagatcg gggaaacgtt cctcgaacga cttggccagt tgcgccaggc
      721 gctcgagcat ggcgtcgttg acgcgcagtt gcgaggcgtc ggcgtcgttc gggcggaagc
      781 gcaaggtcac gtcgaccttg ccgcgcgtga gtttggtcgc aacacgttcg cgcagcgcgg
      841 gttcgattgc gcgcaattcg tccggcaggc gcggattgag ttcgagatag cgatggttga
      901 ccgagcgcaa ttcgaacgca agcgcgcccc acggcgttga agtttccgcg ctggcgaacg
      961 cggtcatgct gcggatcatg gatggctccc cggccatgtt ggcaggcaat ggtaaacttc
     1021 ccgcccgctc ttttgggatt ttttcatggc cgccgccttg atcagtaccg gttcgcgccc
     1081 ctccggccgt gcgccggatc agttgcgcac cgtcgtcatc gaacgccgct acacacgcca
     1141 cgccgaaggc tcggtgctgg tcgcgttcgg cgacacgcgc gtgctttgca cggccagcat
     1201 cgaagacaag gcgccgccgt tcctgcgcgg caagggcgag ggctggatca ccgccgaata
     1261 cggcatgctg ccgcgcgcca ccggcgagcg caccgcgcgc gaagcggcgc gcggcaagca
     1321 aggcggacgc acgctggaaa tccagcgcct gatcgggcgc gcgctgcgtg cctgcgtgga
     1381 tcgcaacgcg ctgggcgaac gcacgatcac gctcgattgc gacgtactgc aagccgacgg
     1441 cggcacgcgc acggcatcca tcaccggcgc ttacgtcgcg ctggtcgatg ccatcaattg
     1501 gctgaaagcg cgcgacaaga t
//
LOCUS       DASRVO010000846         1296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_179539, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000846 DASRVO010000000
VERSION     DASRVO010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179539"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VFF05_10480"
     CDS             <1..999
                     /locus_tag="VFF05_10480"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HZW52280.1"
                     /translation="LHFRNKVAVYTALRSLIDEAALGKDVRAQVLPLRRQANQAARRR
                     LRRPIFIVCPPRSGSTLLFETLAKAPGLATIGGESHGVIEGIGTLSPAARGWDSNRLV
                     AADADLATARALAAAFAERARYRDGLRPDPARTFRLLEKTPKNALRVPFLAEVFGNAS
                     FVYLYRDPRETLSSMLDAWRSGRFVTYPRLPDWPGPPWSLLLTPGWREWRGLDLAQIV
                     ARQWMATVETLIEDLQRLPPERWCVASYSRLVADPQSEIARLCRFLGLRWDHDLKGPL
                     PLSRYTLGAPDPHKWKRNATELESVLPLIEPVAARARDLFALAPEPAAHDDNARQA"
     gene            1067..>1296
                     /locus_tag="VFF05_10485"
     CDS             1067..>1296
                     /locus_tag="VFF05_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HZW52281.1"
                     /translation="MADNSVSRWLRSLAADGRLWWLLLLLPVVLFLPALPIDETRYLA
                     VAWEMRLHLDFLVPHLNGAPYSDKPPLLFWLI"
BASE COUNT          184 a          438 c          439 g          235 t
ORIGIN      
        1 ttgcatttcc gcaacaaggt ggccgtctac acggcgctgc gctcattgat cgatgaggca
       61 gcgctgggca aggacgtgcg ggcgcaagtg ttgccgctgc ggcggcaggc gaaccaggcc
      121 gcacgccggc gcctgcgccg accgattttc atcgtctgtc ccccgcgttc gggctcgacc
      181 ttgttgttcg agaccctggc caaggcgccg ggtctggcca cgatcggcgg tgagagccac
      241 ggcgtcatcg aaggcatcgg cacgctgtcg ccggccgcgc gcggctggga ttcgaaccgc
      301 ctggtcgcgg ccgatgcgga cctggccacc gcgcgcgcac tggcggcggc gttcgccgaa
      361 cgcgcgcgct accgcgatgg cttgagaccg gatcccgcgc ggacgttccg cttgctggaa
      421 aagacgccaa agaacgcctt gcgcgtgccg ttcctggccg aggtgttcgg caacgcctcg
      481 ttcgtgtacc tgtatcgcga tcctcgcgaa accctgagca gcatgctcga tgcgtggcgc
      541 agcgggcgct tcgtcaccta tccgcgtttg ccggactggc ccggaccgcc gtggtcgctg
      601 ctgctgacgc cgggctggcg cgaatggcgc gggctggatc ttgcgcagat cgtcgcacgc
      661 caatggatgg caaccgtcga gaccctgatc gaggatttgc agcgcctgcc gccggagcgc
      721 tggtgcgtgg ccagttacag tcgcctggtt gcggacccgc aatccgagat cgcgcggctg
      781 tgccggtttc tcgggctgcg ctgggaccac gatctgaagg gtccgctgcc gctgtcgcgc
      841 tacacgctcg gcgcgccgga tccgcataaa tggaagcgca atgccacgga gctggaatcc
      901 gtgttgccgc tgatcgagcc ggtcgcggcg cgcgcacgcg acttgttcgc gctcgcgcca
      961 gaacccgccg cgcacgacga caacgcacgg caagcctgag ttttcattcc gccaccgagc
     1021 gggcgcatgg cacaatgcgc gccttgtctc gcgacgtgaa gtgacgatgg ccgacaattc
     1081 cgtttcgcgc tggctgcgct cccttgccgc cgacggccgc ctgtggtggc tgctgctcct
     1141 gctgccggta gtgctgttcc tgccggcgct gccgatcgat gagacgcgtt accttgcggt
     1201 cgcatgggaa atgcgcctgc acctggattt cctcgtgccg cacctcaacg gcgcgccgta
     1261 ttcggacaag ccgccgctgc tgttctggct gatcaa
//
LOCUS       DASRVO010000847         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_207538, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000847 DASRVO010000000
VERSION     DASRVO010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207538"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFF05_10490"
     CDS             complement(<1..180)
                     /locus_tag="VFF05_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HZW52282.1"
                     /translation="MSVASGLRILVLLATVFLGLRSAAAIDPDPAALRRGEFVWHPEI
                     APSGPLVLLVSLDEQR"
     gene            complement(177..1262)
                     /locus_tag="VFF05_10495"
     CDS             complement(177..1262)
                     /locus_tag="VFF05_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52283.1"
                     /translation="MQLILLNTAHLPSRTVWRMKDSTTIDGDASPSPFANAQAVEAWD
                     TWFRWRDDGQLRDVTLEDTWERLASALAGDASRRPGYKRRLIDAFAGWHLLLDERVSA
                     SAGTSAPQWSAHDLHAVLNAASFVHPSGSRHRYFDLAHFEEVAALSVHALDDAAALAA
                     DGSSRSTPGASVGIIGVSNALALLGLAYDSDAGRAQAANIARAMAQGCLSGSIVLARD
                     RGARARCDADWNARAQRRGYRSELIEAAKKHGLRHGRLTAIRSQPRLALFANGVADAL
                     DPLPCTAGANPVTDAACTGVAAQLRLRAAIQPWLDERITCPLSICNEPKASDLETWNT
                     LARQLDLAPLTWRSRNPAAPIQRSADS"
     gene            1380..2177
                     /locus_tag="VFF05_10500"
     CDS             1380..2177
                     /locus_tag="VFF05_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HZW52284.1"
                     /translation="MFNAATSILTAVTEVPSDSAMLGGMSLEELRAHVPAVRRKLRAG
                     QCLHRAGQPFHALYLVTSGFLKTSIIAADGREQITGFCMRGDLMGVESIGMAAYAGDA
                     IALDTSVVWELSYPAVLNACARIPLLQAQLTRALAAQIRSTHSWMLALGTLSAEQRVA
                     AFLLDVAARHAALGFSARQFVLRMSRADMGSFLALKHETVTRALSALSAQGCVCVKWR
                     EVQILDARRLHAIARDGDSAGEETKAGRELRAPSRPAYRGDAVLAAA"
BASE COUNT          389 a          740 c          766 g          380 t
ORIGIN      
        1 gcgttgctcg tcgaggctca ccagcagcac gagcgggccg ctcggcgcga tttccggatg
       61 ccagacgaac tcgccgcgcc gcaacgccgc cggatcggga tcgatggcgg cggcgctgcg
      121 cagtccaagg aacacggtgg ccaggagaac caggatgcgc aagcccgacg cgacactcat
      181 gaatcggcgg agcgctggat cggtgccgcg ggatttcgcg agcgccatgt caggggagcg
      241 aggtcgagct ggcgcgcgag cgtgttccag gtttcgagat cggatgcctt cggttcgttg
      301 caaatgctga gcggacaggt aatgcgctcg tccagccaag gctggatggc ggcgcgcaag
      361 cgcaattgcg ccgcgacacc cgtgcatgca gcatcggtaa ccggattggc accggccgtg
      421 cacgggagcg ggtccagcgc atcggcgacg ccattggcga acaaggcaag gcgcggttgc
      481 gatcggatcg ccgtcaatcg gccgtggcgc aggccatgct tcttcgccgc ctcgatcaat
      541 tcgctgcggt atccgcgccg ttgcgcccgc gcattccagt cggcatcgca tcgggcccgt
      601 gcgccgcgat cgcgtgccaa tacgatcgac ccagacaggc aaccttgtgc catcgctcgc
      661 gcgatgttcg cggcctgggc gcggcccgca tcgctgtcgt aggcgagacc cagcagcgcc
      721 agggcattgc tgacaccgat gatccctacg cttgcgcccg gcgtactgcg cgatgagccg
      781 tcagccgcca gggcggccgc atcgtcaagc gcgtggaccg agagtgcggc cacttcctcg
      841 aaatgcgcaa gatcgaaata ccgatgcctc gatcccgagg gatgcacgaa gcttgccgcg
      901 ttcagaaccg catgcaggtc gtgagcgctc cactgcggcg cggacgtgcc cgcacttgcg
      961 gatactcgtt cgtcgagcag cagatgccag cccgcgaagg cgtcgatgag gcgacgcttg
     1021 taaccgggcc ggcgcgacgc gtcgccggcg agtgccgagg ccaggcgctc ccacgtgtct
     1081 tcgagggtga catcgcgcaa ttgcccgtcg tcacgccagc gaaaccaggt gtcccaggct
     1141 tcgacggcct gagcgttggc aaacgggctg ggcgatgcgt cgccgtcgat cgtcgtcgag
     1201 tccttcatgc gccacaccgt acgcgacggc aagtgcgccg tgttgagcaa gatcaattgc
     1261 atggcgcgtg ccgactccgc cgttacaggc cgttacacag tgtttccgcc gcgtcatgct
     1321 gcggtcgcgg atgcattgga agatgtcgcc gaacccgaat cgcccgcccg gagaacgcca
     1381 tgtttaacgc agcaacatcg atcctgactg ccgttaccga ggtgccgagc gactccgcca
     1441 tgctgggcgg catgtcgctg gaggaactgc gtgcacacgt gcccgcggta cggcgcaagc
     1501 tcagagccgg gcaatgcctg catcgcgccg gccagccgtt ccatgcactg tatctcgtca
     1561 ccagcgggtt tctcaagacc agcatcatcg ccgccgatgg ccgcgaacag attaccgggt
     1621 tctgcatgcg cggcgacctc atgggcgtgg aatcgatcgg gatggcggcc tatgccggcg
     1681 atgcgatcgc cctcgatacc agcgtggtat gggaactgtc ttatccagcc gtgctcaatg
     1741 cgtgcgcgcg catccctctg ctgcaagcgc aattgacgcg tgcgcttgcc gcgcaaatcc
     1801 gcagcacgca cagctggatg ctggctttgg gcacgctcag tgccgaacag cgcgttgccg
     1861 cattcttgct cgatgtcgcc gcacgccatg cggcgctggg tttcagcgcc cggcaattcg
     1921 tgctgcgcat gagccgtgcc gacatgggca gctttcttgc gctcaagcac gaaaccgtca
     1981 cgcgtgcgct gtcggcgttg agtgcgcagg gctgcgtttg cgtgaagtgg cgagaagtgc
     2041 aaatcctcga cgcgcgcagg ctgcacgcca tcgcgcgcga tggggacagc gccggcgagg
     2101 agacgaaagc gggccgggaa ctgcgtgcgc cgtcacggcc ggcttatcgc ggcgatgcgg
     2161 tcttggccgc ggcgtaaacg ccctgcggca cgggcgtcta cgctggtcaa acccgctgta
     2221 cgcgcggggt gaacacgtag cccgcgccac gcacggactt gatcagttcc ggatt
//
LOCUS       DASRVO010000848         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_232845, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000848 DASRVO010000000
VERSION     DASRVO010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232845"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /gene="coq7"
                     /locus_tag="VFF05_10505"
     CDS             <1..547
                     /gene="coq7"
                     /locus_tag="VFF05_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HZW52285.1"
                     /translation="TPAAGIDEADLDDGQRRHAAGLMRINHTGEVCAQALYSGQAAVA
                     RDEQTRSRLLHAAAEETDHLAWCGERLDALASRPSLLNPIWYAGSFAIGAVAALVGDR
                     VSLGFVVETERQVEAHLGEHLEKLPEADTASRAVVAQMQADEARHGRMAKEAGGIELP
                     APVPALMAAASKVMKAVAYRI"
     gene            complement(560..1870)
                     /locus_tag="VFF05_10510"
     CDS             complement(560..1870)
                     /locus_tag="VFF05_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZW52286.1"
                     /translation="MNTSSPAAGNAARNSVFANRDFSLLFGGSSISALGDQFTLVALP
                     WLVLKLTGDPAALGVVLAMMALPRAAFMLIGGAVVDRMSPRRVLLGARAVNAACIVIL
                     AALVLTGTIQMWMVYLLALGIGLSTAFAYPAGSAILPQLVQPQQLRSANALMMGMRQL
                     SMIVGPVLAGFVIATGGHQPSAQGLSDAHGLGFAFGIDAASFLFSLASLAMVRIHGDS
                     HPRKVGAGVFASIAAGFRNLWSDVPLRAFMVYASFVSVFVGGPIQVGLPVLADTRMNL
                     GAASLGILMTAQGIGMFAGSFLSGLGTRLMRGHLGVMVLCGDAVMGLALAALTFVHTT
                     YTGALLLLCTGVLGGIVQIAIFSWIQQRVSQEMMGRTMSILMFTFMGLGPLSAAIAGA
                     LLKVISLTALFGIAGLTLTIIAVSCLSSAQMRSIRATQPMPANS"
     gene            complement(1892..2674)
                     /gene="speD"
                     /locus_tag="VFF05_10515"
     CDS             complement(1892..2674)
                     /gene="speD"
                     /locus_tag="VFF05_10515"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HZW52287.1"
                     /translation="MKPLPRLKLQGFNNLTKALSFNIYDICYAVTPEQRDRYIEYIDE
                     QYDSDRLTKILTDVAEIIGANILNVARQDYDPQGASVTILISEHPVVEKLGRDMISEA
                     VVAHMDKSHITVHTYPETHPHNGIATFRADIDVATCGVISPLKALNYLIDSFESDIVV
                     MDYRVRGFTRDVRGKKHYIDHRINSIQEYLARHIRQKYEMFDVNVYQENMFHTKMHVK
                     EFDLETYLFEAHADDLSFKDRMRIESLLKREIEELFHGRNLT"
BASE COUNT          544 a          872 c          931 g          474 t
ORIGIN      
        1 cacacccgcc gcgggtatcg acgaagcgga cctggatgat gggcagcgcc gccatgccgc
       61 cgggttgatg cgcatcaacc acaccggcga ggtctgcgca caggcgcttt attccgggca
      121 ggccgcagtc gcgcgcgacg aacaaacccg atcgcggcta ctgcatgccg ccgccgagga
      181 aaccgatcac ctggcctggt gcggcgaacg tctggacgcg cttgccagcc ggccgagcct
      241 gctgaatccg atctggtacg ccggcagctt cgccatcggc gcggtcgccg cgctggtcgg
      301 cgacagggtc agcctgggtt tcgtcgtgga aaccgagcgc caggtggagg cgcacctggg
      361 tgagcatctg gaaaagttgc cggaagccga taccgccagc cgcgctgtcg tcgcgcagat
      421 gcaggccgac gaggcgcgtc acgggcgcat ggcgaaagaa gccggcggga tcgaactgcc
      481 ggcaccggtt ccggctctga tggccgccgc gtcgaaggtg atgaaggcgg tggcgtaccg
      541 gatttgaagt gtgtcgcgtt tatgaattcg ccggcatcgg ctgcgtcgct cggatgctgc
      601 gcatctgcgc actgctcagg caggacacgg cgatgatcgt cagcgtcaat ccggcgatac
      661 cgaacagcgc cgtcagggag atcaccttga gcaaggcgcc ggcaattgcg gcggacagcg
      721 ggccaagtcc catgaaggtg aacatcagga tgctcatggt gcgccccatc atttcctgag
      781 agacgcgctg ctggatccag ctgaagatgg cgatctgcac gatgccgcca agcacgccgg
      841 tgcacagcaa cagcaatgcg ccggtgtaag tcgtgtgcac gaacgtcaag gcggcgagag
      901 ccaagcccat cacggcatcc ccgcacaaga ccatgacgcc gaggtggccg cgcatcaggc
      961 gcgtgcctag ccccgagaga aaactgcccg cgaacatgcc gatgccctgt gcggtcatga
     1021 ggatgccgag cgaagccgcg ccaagattca tgcgtgtgtc cgccagaacc ggcaatccca
     1081 cctggatcgg cccgcccacg aacaccgaca cgaaactcgc gtagaccata aacgcgcgca
     1141 gcggcacgtc cgaccacaga ttgcggaagc ccgcggcgat gctggcgaat acgccagcgc
     1201 ccaccttgcg cggatgcgag tcgccgtgga tgcgtaccat cgccagcgac gccagcgaaa
     1261 acaggaaact cgccgcgtcg atgccgaaag cgaaccccag gccgtgcgcg tccgacaatc
     1321 cttgcgctga gggctggtgc ccgcccgtcg cgatgacgaa gcccgcgagc accggaccga
     1381 caatcatgct gagctggcgc atccccatca tcaaggcgtt ggcgctgcgc aactgttgtg
     1441 gttgcaccag ttgcggcagg atcgcggaac ctgccgggta cgcgaaggcc gtggacaggc
     1501 cgatgccgag cgccagcaga tagaccatcc acatctgaat cgtgccggtc agcaccagcg
     1561 ccgcgagaat cacgatgcag gcggcgttga cggcccgcgc acccagcagc acgcgccgcg
     1621 gcgacatgcg gtcgacgacc gcgccgccga tcagcatgaa ggccgcgcgc ggcagcgcca
     1681 tcatcgccag caccacgccg agtgcggcag ggtcgccggt tagtttcagc accagccacg
     1741 gcaacgccac cagcgtgaac tggtcgccca acgcggaaat cgaactgccg ccgaacagca
     1801 ggctgaaatc gcggttggcg aatacggaat tgcgcgcggc gttccccgcc gccggagagg
     1861 atgtgttcat tggcgcacta tcttgcggcg atcaggtcaa atttcgcccg tggaacagct
     1921 cctcgatctc gcgcttgagc agcgattcga tgcgcatgcg gtccttgaac gacagatcgt
     1981 cggcgtgggc ttcgaacagg taggtctcga ggtcgaattc cttcacgtgc atcttcgtgt
     2041 ggaacatgtt ttcctgatac acgttgacgt cgaacatctc gtatttctgg cggatgtgcc
     2101 gggccaggta ttcctggatc gagttgatgc ggtggtcgat atagtgtttc ttgccgcgca
     2161 cgtcgcgcgt gaagccgcgc acgcggtagt ccatcaccac gatgtcggat tcgaaactgt
     2221 cgatcaggta gttcagggcc ttcagcggcg aaatcacgcc gcaggtggcc acgtcgatgt
     2281 cggcgcggaa cgtggcgatg ccgttgtgcg gatgcgtctc cgggtaggtg tgcacggtga
     2341 tgtgcgattt gtccatgtgc gcgactacgg cttcggaaat catgtcgcgg ccgagcttct
     2401 ccaccaccgg gtgctcggag atcaggatcg tcacgctcgc gccctgcgga tcgtaatcct
     2461 gacgcgcgac gttgaggatg ttcgcgccga tgatctcggc cacgtcggtg aggatcttgg
     2521 tcaggcggtc cgaatcgtat tgctcgtcga tgtactcgat gtagcggtcg cgctgttcgg
     2581 gcgtcaccgc gtagcagata tcgtagatgt tgaaggacag cgccttggtc aggttgttga
     2641 agccctgcag cttcaggcgg ggaaggggtt tcactgcgca gactccgaaa accggccggg
     2701 acgccgtcag ggcgcgaatt atggagcaat cggtcggaag gggaaaccgt tttcgcggtc
     2761 ttgcgcagcg caagcgggat tccgataaga tgacgcatcg tcggcgccgc atcgccgcgt
     2821 a
//
LOCUS       DASRVO010000849         4846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_207598, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000849 DASRVO010000000
VERSION     DASRVO010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207598"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VFF05_10520"
     CDS             complement(<1..132)
                     /locus_tag="VFF05_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010483909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZW52288.1"
                     /translation="MTRTITVIPGDGIGPEIMRATLRVLDELKTGLQYEVVEAGVAAL
                     "
     gene            302..1330
                     /locus_tag="VFF05_10525"
     CDS             302..1330
                     /locus_tag="VFF05_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52289.1"
                     /translation="MLGAAGSASVSSHDSTVANAILPIWSLSIAGQWATQCPPVLQSV
                     ALDGNDLRIDARSVLDLCAAQPTPYAIELNPALALQHAAFAPGVYRVSFYAADGAQVQ
                     PKLRAFALIDRSGRDAETTTPETGFWSSDGDDRTLLSLELQGGQLSVALLSYDADGQP
                     AWLFGSAPYDGRIAHVSMLRLSGGSAPFAPAPASPHGDAELTLDLQFSTSAHAQAWLS
                     RMHGDGSLQLRALDISRLPLSASVDGSAWQGEWVLVGDAENVATQRLRLSQFHALDAG
                     RFQLTTADATIALDCERALTQPEQPPRSCSLHMEDGSTSRFDSVAIGRMDGADANGAA
                     IHLLRIKP"
     gene            complement(1320..1631)
                     /locus_tag="VFF05_10530"
     CDS             complement(1320..1631)
                     /locus_tag="VFF05_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZW52290.1"
                     /translation="MNERILEHDNQVVRRFFALDTQTYKGGALPAQTKEMLGLVASLV
                     LRCDDCIGYHIAECRNAGVTRDEFFEIFSVGLVVGGSIVIPHLRRAVDFLDELENGAK
                     V"
     gene            complement(1673..1933)
                     /gene="grxC"
                     /locus_tag="VFF05_10535"
     CDS             complement(1673..1933)
                     /gene="grxC"
                     /locus_tag="VFF05_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HZW52291.1"
                     /translation="MPKIEMYTSANCAYCVAAKNLLKSKGLDYQEIRIDTDPARREEM
                     LTRTRRRTVPQIFVNDRHVGGYDDLVAVERSGELAKLIGGGT"
     gene            2088..3755
                     /locus_tag="VFF05_10540"
     CDS             2088..3755
                     /locus_tag="VFF05_10540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZW52292.1"
                     /translation="MRISPAIAALVLACTLLAGKPVRAVDTKLPDMGSSAATIASPEE
                     QRQYGFYVLHELRNQGMVLDDALLTDYINTLGYRLVSYSPQPDQPFTFFIVNDPSINA
                     FALPGGFVGVNAGLITTTTGEDELAAVLAHEISHITQQHLVRAVEAEQKYAPLMVLAM
                     VGAVIASAKSSPNSTSNADMGAIATATGLAQQMQINFTRADEEEADRVGIQTLAKANF
                     DPDAMAEFFQRMQQALRPGFDENDVPALLMDHPVTLKRISEAKERALVVKKEETQRRA
                     ALASARTVGAQVSPLLRFPGNRMSSSAMAAELARPSETPAQQHARSEAYYELMRERVR
                     VLASNQPTQTATYYSDNLKNMQGFGTIPNRYGQVLALVRAGDAKKAVPLADKLASEHP
                     DQLVLQLAAGSAEAMSDARAAALKRYTSLEGDYPDNRALVLAHAQALLQVNDLASARL
                     AQKLLRPQLSNDDEDPDLQTVFARACELAGDRVRAGEAHADAAYLNGRAEDALNQLKD
                     LTKRSDLDYYQRARIEARIAEITPVVLELRRRKIKPEDQGNLALQPN"
     gene            3794..>4846
                     /locus_tag="VFF05_10545"
     CDS             3794..>4846
                     /locus_tag="VFF05_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HZW52293.1"
                     /translation="MTVRRKPQTPELHLNRELAQLEFNVRVLAQAQDAQVPLLERVRY
                     LCIANSNLDEFFEVQVAILHHHVAFGDAVPGPDGLPPTAALGRIRTRALELVRDQYAM
                     WNEALLPELDREGIRFPERASWTARQRDWLQNYFRSEVLPVLSPLGLDPAHPFPRILN
                     KSLNIAVALRGTDAFGREGHVALVRAPRSLPRLVRLPPEICAQPYEFVFLSSILLQFV
                     DELFPGMRVKGAYPFRVTRNSELFVDEEEIEDLAHALRDELRGRGFARAVRLEISETC
                     PKSVADFLMRNFELDETQVYRCNGPVNIHRVAAIYDQIERADLKFPRFTPRMPDALAA
                     ERNLFEAIAERDILLHH"
BASE COUNT          867 a         1658 c         1479 g          842 t
ORIGIN      
        1 cagcgcggca acgccggctt cgacaacttc gtactgcaat ccggttttca gttcgtcgag
       61 tacgcgcagc gtcgcgcgca tgatttccgg gccgatgccg tcgcctggaa ttacggtaat
      121 ggttcgcgtc atgaaaagtc ggcgttaaca agaaataaat tagccgacta ttatcgtgta
      181 gactcgccgc caggacaaga aatactgtcg tgaatctgcc gttgtgaaac cgcgccctgc
      241 cacgttgcga accgtctgct gcctgctcgc ggcggtattc gttggtccgg cgtacgcgca
      301 aatgctcggc gctgccggct cggcatcggt ctcctcgcac gattcgaccg tagccaatgc
      361 catcctgccg atctggagcc tttccatcgc gggacagtgg gccacgcagt gcccgccggt
      421 gctgcaatcg gtggcgctgg acgggaacga tctgcgcatc gacgcgcgtt ccgtgctcga
      481 tctttgcgca gcgcagccga cgccctacgc catcgaactc aatccggcgc ttgccttgca
      541 gcatgcggcg ttcgcgcccg gcgtttatcg cgtcagtttc tacgccgccg acggcgcgca
      601 agtgcagccg aaactgcgcg ccttcgccct gatcgatcgc tccgggcggg acgccgaaac
      661 gacgactccg gaaaccggtt tctggtcgag cgacggcgac gatcgcacct tgctcagtct
      721 tgagcttcag ggcggccagt tgagcgtggc gctgctcagc tacgacgccg acggtcaacc
      781 cgcctggctt ttcggcagcg cgccgtatga cggtcgcatc gcccacgtct ccatgctgcg
      841 cctgtccggc ggcagcgcgc cgttcgcgcc cgcgccagca tcgccccacg gcgacgcgga
      901 gttgacgctg gatttgcagt tttccacgtc ggcgcacgct caggcctggc tgtcgcggat
      961 gcacggcgac ggttcgctgc aactgcgcgc gctcgacatc tcgcgcctgc cgctgtcggc
     1021 cagcgtcgac ggcagcgcct ggcagggcga atgggtattg gtcggagacg ccgaaaacgt
     1081 cgcaacgcag cgcctgcgcc tgagccagtt ccatgcgctc gacgcgggcc gctttcagct
     1141 cacgacagcg gacgcaacca tagcactcga ttgcgaacgt gcgttgacgc aaccggagca
     1201 accgccgcga tcgtgcagcc tgcatatgga ggacggatcg acgagtcgct tcgactccgt
     1261 ggcgattggc cgcatggatg gcgccgacgc gaacggcgcg gcaatccacc tgttgcgaat
     1321 caaaccttag cgccgttttc caactcgtcg agaaaatcca ccgcgcggcg caggtgcggg
     1381 atcacgatcg aaccgccgac gacaagaccg acgctgaaga tctcgaagaa ctcgtcgcgc
     1441 gtcacgccgg cgttcctgca ttcggcgatg tggtagccga tgcaatcgtc gcagcgcagc
     1501 acgagcgagg cgacaagacc cagcatttcc ttcgtctgcg ccggcagcgc accgcccttg
     1561 taggtttgtg tgtccagcgc gaagaaacgg cgcacgacct ggttgtcgtg ctcgaggatg
     1621 cgctcgttca tgcgcttgcg gaacgccacg aattcatcca cacgctttgc gctcacgtcc
     1681 cacccccaat cagtttggcc agttcgccgc tgcgttcgac cgcgacaaga tcgtcgtagc
     1741 cgccgacatg gcgatcattt acgaaaattt gcggcaccgt ccgccgccgc gtgcgggtga
     1801 gcatctcctc gcgccgcgcc ggatccgtat cgatgcggat ttcctgatag tccagcccct
     1861 tgcttttgag caggttcttg gccgccacgc agtaggcgca gttggcgctg gtgtacattt
     1921 cgatcttggg catgccgatt accgtgtgtc gattgcggcc attttcccac gtcgccgcgg
     1981 ctacgtctgt gccggggatt aacgccggcc aaacacgcga cgcgcaacaa tggccgcgag
     2041 ccgccggctg cgctatcgtg acgcttccgc acgcggcttt ttcgaacatg cgcatttctc
     2101 ccgccatcgc tgccctggtc ctggcctgca ccctgctcgc cggcaagccc gtgcgcgccg
     2161 tggataccaa gctccccgac atgggcagct cggccgcgac gatcgcctcg cccgaggaac
     2221 agcgccaata cggtttctac gtcctgcacg aactgcgcaa tcagggcatg gtgctggacg
     2281 acgccctgct caccgactac atcaatacgc tcggctatcg cctggtttcc tacagcccgc
     2341 agccggatca gccgttcacg tttttcatcg tcaacgatcc gtcgatcaac gcgttcgccc
     2401 tgcccggtgg attcgttggc gtcaacgcgg ggctcatcac cacgaccacg ggcgaggatg
     2461 aactcgccgc cgtgctcgcg cacgagattt cgcacatcac ccaacagcat ctggtgcgcg
     2521 ccgtcgaggc cgagcagaaa tatgcgcctc tgatggtgtt ggcgatggtc ggcgcggtca
     2581 tcgcctcggc gaaatccagc cccaacagca ccagcaacgc ggacatgggc gccatcgcca
     2641 cggcaaccgg cctggcgcag cagatgcaga tcaacttcac ccgcgccgac gaggaagaag
     2701 ccgatcgcgt cggcatccag accctggcca aggcgaattt cgatcccgac gcgatggccg
     2761 aattcttcca gcgcatgcag caggcactgc ggccgggttt cgacgaaaac gacgtgccgg
     2821 cgctgctgat ggatcatccg gtcacgctca agcgcatcag cgaagccaag gaacgcgcgc
     2881 tggtcgtgaa gaaggaagag acccagcgcc gcgcggcgtt ggccagcgca cgtaccgttg
     2941 gcgcgcaggt gtcgccgttg ttgcgctttc ccggcaaccg catgagttcc agcgcgatgg
     3001 cggcggaact ggcacggcct tccgaaactc cggcgcaaca gcacgcgcgt tcggaggcgt
     3061 attacgagtt gatgcgcgaa cgcgtgcgcg tgctggcctc gaaccagccg acgcaaaccg
     3121 cgacctacta ttcggataac ctgaaaaata tgcaggggtt cgggacgatt cccaaccgct
     3181 acggccaggt gctcgcgctg gtgcgtgccg gcgacgcgaa aaaagccgtg ccgttggccg
     3241 acaaactcgc cagcgagcat ccggatcaac tggtgttgca gttggccgcg ggcagcgccg
     3301 aggccatgtc cgacgcgcgc gctgcggcgc tgaagcgcta cacgtcgctg gaaggcgatt
     3361 atcccgacaa ccgcgccctc gtgctggcac atgcgcaggc gctgctgcag gtcaacgatc
     3421 tggccagcgc caggcttgcg cagaaactcc tgcgcccgca actctcgaac gacgacgagg
     3481 atcccgacct gcaaaccgtg ttcgcgcgcg cctgcgaact tgccggcgat cgcgtgcgcg
     3541 ccggcgaggc gcacgccgac gccgcctatc tgaacgggcg agccgaagat gcgctcaacc
     3601 agctcaagga cctcaccaag cgcagcgacc tcgactacta ccaacgcgcg cgcatcgagg
     3661 cgcgcatcgc cgaaatcacg ccggtcgtgc tggaactgcg ccggcgcaag atcaaaccgg
     3721 aagaccaggg caatctggcc ctgcaaccga actgaaacct gcgcctgcgc ggtttcggta
     3781 tcatgccgga cagatgaccg tccgacgcaa gccccagact ccggaactgc acctcaaccg
     3841 cgaactggcc caactcgagt tcaacgtgcg cgtgctggcg caggcgcagg atgcgcaggt
     3901 gccgctgctc gagcgcgtac gctatctgtg catcgccaat tccaacctcg acgagttttt
     3961 cgaggtgcag gtcgcgatct tgcaccacca tgtcgcgttc ggcgacgcgg ttcccggccc
     4021 cgatggattg ccgcccacgg cggcgctcgg ccgcatccgc acgcgcgcgc tggaactggt
     4081 gcgcgaccag tacgccatgt ggaacgaggc gctgctgccg gaactggatc gcgaaggcat
     4141 ccgttttccc gagcgcgcga gctggaccgc caggcaacgc gattggctgc agaattattt
     4201 ccgcagcgaa gtgctgccgg tgctgtcgcc gctcggactc gaccccgcgc atccgttccc
     4261 gcgcatcctc aacaagagtc tgaacatcgc cgtcgccctg cgcggcaccg acgcgttcgg
     4321 ccgcgaaggt cacgtcgccc tggtgcgtgc gccgcgctcg ctgccgcgcc tggtgcgcct
     4381 gccgccggaa atctgcgcgc aaccctacga attcgtgttc ctgtccagca tcctgctgca
     4441 gttcgtcgac gaattgttcc cgggcatgcg cgtcaagggc gcgtatccgt tccgcgtcac
     4501 ccgcaacagc gaactgttcg tcgacgagga ggaaatcgag gacctcgccc acgccttgcg
     4561 cgacgaattg cgcggacgcg gtttcgcgcg cgcggtgcgc ctggagattt ccgaaacctg
     4621 cccgaagagc gtcgccgact tcctgatgcg caacttcgag ctggacgaaa cccaggtcta
     4681 ccgctgcaac ggcccggtca acatccaccg cgtcgccgcg atctacgacc agatcgagcg
     4741 cgcggacctg aaattcccgc gcttcacgcc gcgcatgccg gatgctctcg ccgccgagcg
     4801 caatctgttc gaggcgatcg ccgagcgcga catcctgctg caccat
//
LOCUS       DASRVO010000850         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_102555, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000850 DASRVO010000000
VERSION     DASRVO010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102555"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VFF05_10550"
     CDS             <1..920
                     /locus_tag="VFF05_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZW52294.1"
                     /translation="NKLAQEQSNADALNVDYKRAQIDSRKQHLELQKTLDNAVIDAKS
                     AQTDLERNQKAFAQGAVAQMQVAHAQDALDKAHVALRHAQQDMGLDNDSLHFNVESKK
                     LAWERQVLFVKDLQRQVDELNVRSPVNGQVGQLFVAPSATVAKDAKLLSVVDLSALEV
                     EVKVPESFARDLAIGMSAQITGNGSQWPGTVGAISPEVVNGEVAARVRFSGDQPAHLR
                     QNQRLSVRIVLDQRDDALTVARGSFVDESGGRYAYVVRDGIAVKTPVRLGVSSIDKVE
                     ILDGLKVGDRVVISGTSNFNDAPRIAISN"
     gene            977..1690
                     /locus_tag="VFF05_10555"
     CDS             977..1690
                     /locus_tag="VFF05_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZW52295.1"
                     /translation="MLTMTHLAKVYRTELVETYALRDFNIDVKEGEFVAVTGPSGSGK
                     TTFLTIAGLLETFSGGEYHLDGIEVSNLDDNARSRIRNEKIGFIFQAFNLIPDLNVFD
                     NVDVPLRYRGMKADERRRRIMDSLERVGLAARAKHYPAELSGGQQQRVAIARALAGSP
                     RLLLADEPTGNLDTQMARGVMELLEEIHRDGATIVMVTHDPELAARAQRNVHIIDGQV
                     VDIAEEPRFHAHAAKTAHA"
     gene            1714..>1922
                     /locus_tag="VFF05_10560"
     CDS             1714..>1922
                     /locus_tag="VFF05_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52296.1"
                     /translation="MNARKYLIALAIAISITGLEFVGLSVLADSAGRSARHAFVVPEA
                     AIPMLPVITVQPTHEEVRAAFAGTDL"
BASE COUNT          392 a          646 c          593 g          291 t
ORIGIN      
        1 ccaacaagct cgcgcaggag caatccaacg ccgatgccct gaatgtcgac tacaagcgcg
       61 cgcagatcga ttcgcgaaag cagcacctcg aattgcagaa gacgctggac aacgccgtca
      121 tcgacgcgaa gagcgcgcag accgacctcg agcgcaacca gaaggcgttc gcacagggcg
      181 cggtggcgca aatgcaggtg gcgcatgcgc aggacgcgct ggacaaggcg catgtcgcgc
      241 tcaggcacgc gcagcaagac atgggcctgg acaacgacag cctgcacttc aacgtcgagt
      301 cgaagaagct cgcctgggag cgccaggtgc tgttcgtgaa ggacctgcag cgccaggtcg
      361 acgaattgaa cgtgcgctcg ccggtgaacg gccaggttgg ccagttgttc gtcgcgccga
      421 gcgcaaccgt ggccaaggac gccaagctgc tcagcgtggt cgacctgtcc gcgctcgaag
      481 tcgaggtgaa ggtgccggaa agtttcgccc gcgacctcgc catcggcatg agcgcgcaga
      541 tcaccggcaa cggcagccag tggccgggca ccgtcggcgc gatttcgccg gaagtggtga
      601 atggcgaagt cgccgcgcgc gtgcgcttca gcggcgatca gccggcgcac ctgcgccaga
      661 accagcgcct gtcggtgcgc atcgtgctcg accagcgcga cgatgcgctc accgtcgcgc
      721 gcggttcgtt cgtcgacgaa tccggcggcc gttatgccta cgtggtgcgc gacggtatcg
      781 cggtgaagac gccggtgcgc ctgggcgtga gttccatcga caaggtggaa atcctcgacg
      841 gcctgaaagt cggcgaccgc gtggtgattt ccggtaccag caatttcaac gatgcgcccc
      901 gaatcgccat cagtaattag tggattcatc catcaacgca agcatattca actcacgctc
      961 gaaggaaacc aaagccatgc tcacgatgac tcatcttgcc aaggtctacc gcacagaact
     1021 cgtggaaacc tacgcgctac gcgacttcaa catcgacgtg aaggaaggcg agttcgtcgc
     1081 cgtgaccggg ccctcgggct cgggcaagac cacattcctg accatcgccg gcctgctcga
     1141 gacgttcagc ggcggcgagt accacctcga cggcatcgag gtcagcaatc tcgacgacaa
     1201 cgcgcgctcg cgcattcgca acgagaagat cggtttcatt ttccaggcct tcaacctgat
     1261 tccggacttg aacgtgttcg acaacgtcga cgtgccgctg cgctaccgcg gcatgaaggc
     1321 cgacgagcgc cgccgccgca tcatggattc gctcgagcgc gtgggcctag ccgcccgcgc
     1381 caagcactat ccggcggaac tttccggcgg ccagcagcaa cgcgtcgcca tcgcccgcgc
     1441 actggccggc tcaccgcgcc tgctgctcgc cgacgagccg accggcaacc tcgatacgca
     1501 gatggcgcgc ggcgtgatgg aactgctgga ggagatccac cgcgacggcg cgaccatcgt
     1561 catggtcacg cacgatccgg aacttgccgc gcgcgcgcaa cgcaacgtcc acatcatcga
     1621 cggccaggtc gtcgacatcg ccgaagagcc gcgcttccac gcgcatgccg cgaaaacggc
     1681 ccacgcgtga accgcatcac gaggagaacc atcatgaacg ctcgcaaata cctgatcgca
     1741 cttgccatcg ccatatcgat caccggactg gaattcgtcg gcctgagcgt gctcgccgac
     1801 agcgccggcc gatccgcacg ccacgccttc gtcgtgccgg aagcggcgat tccgatgctg
     1861 ccggtcatca ccgtgcagcc gacgcacgag gaagtgcgcg ccgcctttgc cggcaccgac
     1921 ct
//
LOCUS       DASRVO010000851         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_102566, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000851 DASRVO010000000
VERSION     DASRVO010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102566"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFF05_10565"
     CDS             <1..501
                     /locus_tag="VFF05_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZW52297.1"
                     /translation="IRIVMLTGDNPTTAAAIARRLGIGEFRAGVLPEDKARIVSALRA
                     EGRVVAMAGDGVNDAPALAAADVGIAMGTGADVALESAGVTLVKGDLAGILRARHLSR
                     AAMRNIRQNLFFAFVFNALAIPIAAGVLYPLWGLLLSPMIASAAMSLSSVTVIGNALR
                     LRRVRL"
     gene            676..1695
                     /locus_tag="VFF05_10570"
     CDS             676..1695
                     /locus_tag="VFF05_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZW52298.1"
                     /translation="MDKISTIGLDLAKSVFQVHGVNEAGKVVVERKLRRGQLLAWFAT
                     LPPCLIGIEACGTAHYWGRELTKLGHAVRLIPPAYAKAYVRRNKNDAADAAAICEAVS
                     RPAMRFVALKSEAQQALAGLHRVRDLLMKQRTMLRNQLRSLMAELGVAAAQGRRGLNE
                     LLAVLGDAADQRIPALLRKALLASAEVLRDLERKIEALEAQMVAAGREDATYRHLITA
                     AGYGPILSSAMAAMVTDPAAFRSARDFAASLGLVPRQDGTGGKVRLGPISKRGNGYLR
                     RLLVNGAASLIISGRAKRDPWLERLLAAKPRKVAMVALANKLARIGWALMSRQQDYRF
                     VPAAA"
     gene            2045..>2244
                     /locus_tag="VFF05_10575"
     CDS             2045..>2244
                     /locus_tag="VFF05_10575"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW52299.1"
                     /translation="MTLSDDIKDMIEHDNARARRLLHGIVTEAPHSVEARFLLAQAYL
                     RRMEVERALPLYLDVTARDPKLA"
BASE COUNT          371 a          731 c          760 g          382 t
ORIGIN      
        1 atccgtattg tcatgctcac gggcgacaac cccaccaccg ccgcggcgat tgcgcgccgg
       61 ctcgggattg gcgaattccg cgccggtgtg ctgccggaag acaaggcgcg gatcgtgagc
      121 gcgctgcgcg ccgagggccg cgtcgtcgcg atggcgggcg atggcgtcaa cgatgcgccg
      181 gcgctggccg cggcggatgt cggcatcgcc atgggcacgg gcgccgatgt cgcgcttgaa
      241 agcgcgggcg tgactttggt caaaggcgat ctcgccggca tcctgcgcgc gcggcacctc
      301 tcgcgcgcgg cgatgcgcaa cattcgccag aatctgttct ttgccttcgt cttcaacgcg
      361 ctcgccattc cgatcgcggc gggcgtgctc tatccactct ggggcttgct gctcagcccg
      421 atgatcgcca gcgcggcgat gagcctcagc tccgtcaccg tcatcggcaa cgcgctccgt
      481 ttgcggcggg tacggcttta gtcatgcatt tggggcgtca ttgcgaggcc gagcttgcga
      541 ggccgaagca atccagagcc ggtgctcagc ctggattgcc gcgtcgctgc gctcctcgca
      601 atgatgaggt ggatggcccc cttacggcat cgatgtgcca aagttgagtt gcaaccaact
      661 tacggggagc catccatgga caagattagc acaattggtc tcgatctggc gaagtcggtg
      721 tttcaggtgc acggcgtgaa tgaagctggc aaagttgtgg tggaacggaa gctgcggcgc
      781 ggtcagctgc tggcgtggtt cgcgacgctg ccgccgtgcc tcattgggat cgaggcgtgc
      841 gggacggcgc attactgggg gcgggagctg acgaagctgg ggcacgcggt gcggctgatc
      901 ccgccggctt acgccaaggc ctatgtacgg cggaacaaga acgatgcggc ggatgcggcg
      961 gcgatctgcg aggcggtgag ccgacccgcg atgcgcttcg tcgcgctcaa aagcgaagca
     1021 cagcaagcgc tggccgggct tcatcgcgtg cgcgacctct tgatgaagca acgcacgatg
     1081 ctgcgcaatc agctgcgcag cctcatggcc gagctcggcg tggcggcggc gcaaggccgg
     1141 cgcgggctga acgagctgct ggccgtgctg ggcgatgctg cggaccagcg catcccggct
     1201 ctgctgcgca aggcgctgct cgcgagcgcg gaggttctgc gcgatcttga gcgcaagatc
     1261 gaggcgctgg aggcgcagat ggtggcggca ggccgcgagg atgcgaccta ccggcatctc
     1321 atcaccgccg caggctatgg tccgatcctc tccagcgcga tggcggcgat ggtgaccgat
     1381 cccgccgcct tccgctccgc ccgcgacttc gccgcctcgc tcgggctggt gccgcgccag
     1441 gacggcaccg gcggcaaggt gcggctcggc ccgatcagca agcgtggcaa cggttatctg
     1501 cgccgcctcc tcgtcaacgg cgccgcctcg ctcatcatca gcggccgcgc caagcgcgat
     1561 ccctggcttg agagactgct cgccgccaag ccgcgcaagg tcgccatggt cgcgctcgcc
     1621 aacaagctcg cccgcatcgg ctgggcgctg atgagccggc agcaggatta tcgcttcgtc
     1681 ccggcagcgg cctagccgcc gccggcatac ggtccgcaag cttgcgaggg tgaaacacgc
     1741 gtgatggcaa tgtcggtcaa tccgacgatc gagggtaacc tgttacttgt catggggcat
     1801 cgcgacctcg ccgcaatgat caggcctcga tccacggatc ccatcagggc ccgcggccat
     1861 atcgcgccgc atcacgaggc cgaacacatg accgcactgg ccgcgtccaa cacgcgcaaa
     1921 aatcccttgc caagcggggg ccatccacac atgacactgt ttttggattc tcgcggtcga
     1981 tccattcacc ggctcttaat cgcgcccggc ctataaggca ggctcagcct cagaccgggc
     2041 cgtcatgacg ctcagcgacg acatcaagga tatgatcgag cacgacaatg cgcgtgcgcg
     2101 ccgcctgctg cacggcatcg tcaccgaggc gccgcattcg gtcgaagcgc ggttcctctt
     2161 ggcgcaggcc tatctccggc gcatggaggt ggagagagcg ctgccgctct atctggacgt
     2221 gacggcgcgt gacccgaaat tggc
//
LOCUS       DASRVO010000852         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_25186, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000852 DASRVO010000000
VERSION     DASRVO010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25186"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /gene="rpsR"
                     /locus_tag="VFF05_10580"
     CDS             complement(<1..223)
                     /gene="rpsR"
                     /locus_tag="VFF05_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020584246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZW52300.1"
                     /translation="MSKFFRRKKYCRFTAEGVTEIDYKDLNTLRQYIGETGKIVPSRI
                     TGTKARYQRELTQAIGRARFLALLPYTDSH"
     gene            complement(239..691)
                     /gene="rpsF"
                     /locus_tag="VFF05_10585"
     CDS             complement(239..691)
                     /gene="rpsF"
                     /locus_tag="VFF05_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZW52301.1"
                     /translation="MRHYEIVFLVHPDQSEQVPAMIERYKTLIEGANGKIHRLEDWGR
                     RQLAYPINNLAKAHYVLMNIECGQDVLSELQSGFRFNDAVLRHLIIAKDGPVTEQSFI
                     LKNKDDKGGKRRDDEGFGFGDDDDQPRRERTPRPAPVAAASAAEPAAE"
     gene            complement(797..1648)
                     /locus_tag="VFF05_10590"
     CDS             complement(797..1648)
                     /locus_tag="VFF05_10590"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZW52302.1"
                     /translation="MSLSTLIDAQTLHSRVRAGDVLVVDCHFELADPGRAAHDYAQGH
                     IPGAVYASLDDDLSDLSKQNLGRHPLPDAVAFSATLSRWGWTRDMPVVAYDAANGALA
                     AARLWWMLRLVDAREAAVLDGGIAAWTRAGFALDASVVRREPTRVDVAFDPAQVVYTD
                     VLVEALKDARSLLVDARAAPRYRGEVEPLDKAAGHVPGAVNRPFADNLDASGRFKPAA
                     QLRAEFAQLLGSHRPVDTIHMCGSGVTACHNLLAMEQAGLAGSRIYAPSWSGWTSDPS
                     RPVATNS"
     gene            complement(1645..2477)
                     /locus_tag="VFF05_10595"
                     /pseudo
     CDS             complement(1645..2477)
                     /locus_tag="VFF05_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036720.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
BASE COUNT          468 a          859 c          900 g          476 t
ORIGIN      
        1 agtggctgtc cgtgtacggc agcaaggcaa ggaagcgcgc gcggccaatc gcctgggtca
       61 attcgcgctg atagcgcgcc ttggtgccgg tgatgcggct gggcacgatc ttgccggttt
      121 cgccgatgta ctggcgcaac gtgttcagat ccttgtaatc gatctcggtc acgccttcag
      181 cggtgaagcg gcagtatttc ttgcggcgga agaatttgga catggtcgtt ctcctggctt
      241 attcggcggc gggttcggca gcggacgcgg cggcaaccgg agccgggcgc ggcgtgcgtt
      301 cgcgacgcgg ctggtcgtca tcgtcgccga agccgaagcc ttcgtcgtcg cggcgcttgc
      361 cgcccttgtc gtccttgttc ttgagaatga acgactgctc ggtgaccgga ccgtccttgg
      421 cgatgatgag gtggcgcagc acggcgtcgt tgaagcggaa gccggattgc agttcgctca
      481 gcacgtcttg cccgcactcg atgttcatga gtacgtaatg cgccttggcc aggttgttga
      541 tgggatacgc gagttggcgg cggccccagt cctcgaggcg atggatcttg ccattggcgc
      601 cttcgatcag cgtcttgtaa cgctcgatca tcgcgggcac ctgctcgctc tggtcagggt
      661 ggaccagaaa cacgatctca tagtgacgca tgttgtctcc ttgtggatac cgcgaagggg
      721 aattcctgcg cgatagcctc ccgcggcgcg gtgaggcaag gtccccgcaa attgcggggc
      781 gcgaattgta attgaatcaa gagtttgtgg caacaggccg ggacgggtcc gatgtccagc
      841 cggaccacga tggcgcgtaa atgcgcgatc cggccagccc ggcctgctcc atcgccagca
      901 agttgtggca ggcggtgacg cccgaaccgc acatgtggat ggtgtcgacc ggacgatgcg
      961 atccgagcaa ttgcgcgaac tcggcacgca actgcgcggc cggcttgaag cggccgctgg
     1021 cgtcgagatt gtccgcgaac gggcggttca ccgcgccggg aacgtgcccg gcggccttgt
     1081 ccagcggttc gacctcgccg cggtagcgcg gcgcggcgcg cgcgtcgacg agcaagctgc
     1141 gcgcgtcctt cagcgcttcg accagcacgt cggtgtagac gacctgcgca gggtcgaagg
     1201 ccacgtcgac ccgggtaggc tcgcgccgca cgacgcttgc atccagcgcg aatccggctc
     1261 gcgtccacgc cgcgatgccg ccgtcgagca cggccgcctc gcgcgcgtcc acgaggcgca
     1321 gcatccacca cagccgcgcc gcggccaggg ccccgttcgc ggcgtcgtag gccacgaccg
     1381 gcatgtccct tgtccagccc cagcgcgaca acgtggccga gaacgccaca gcgtcgggca
     1441 agggatggcg gccgaggttt tgtttggaca aatccgaaag atcgtcatcg aggctggcat
     1501 acaccgcgcc ggggatgtga ccttgcgcat agtcgtgtgc cgcgcgtccc ggatcggcca
     1561 actcgaaatg acagtcgacg acgagtacgt cgccggcgcg tacccgcgaa tgcagggttt
     1621 gtgcgtcgat caatgtggaa aggctcatgg tgtcgcctcg atgcgccgca ccaggctcaa
     1681 cagcatggcc gcggtcgcac cccagatgat gcggtcgttg tatgcaaact cgaagacatc
     1741 gcgggttcgc ccctgccagt ccaagcggcg ctggcgcagg ttggccggat cgaggaagaa
     1801 cgccagcggc gcctcgaaca cgtcggcgac ttcgtgcgga tccggcgtcg cgcggtaggc
     1861 cggatcgatt tgcgcgacga ccggcgtgac gcagtaaccg ctgatcgtct cgaaacaatc
     1921 caggtacccg aacggctgca ccagcgcggc atcgatgccg acttcctcgc gtgtttcgcg
     1981 caatgccgcg gcgacggaat tcgcgtccgc ggactcgatg ccgccgcccg gaaaactcac
     2041 ctgccccgcg tgctgcgcca attgctcgtt gcgtcgcgtg aacaggatcg aaggcccatc
     2101 cgcgcgcgcg atcgtcgcca ccagcaccgc ggcggcgcgg cgcggacccg gaccgagcag
     2161 gtcggcgatc tcggcgtggt tccaaccggg tgcggacggc gggtcttgca acgtgcgcac
     2221 ggcacggcgc acgcgtgcgt cgagttcgct cctggcgaaa tccggcgcgg cgctcatgcg
     2281 gattcctcgt gcgcagccgc gcgtcgcggc agcacatcgt tcatcagatg caaacgttgc
     2341 gcatcgctga gttgcaacca gcgcgcgatt tcgtcgcaat tacgatgaca gcccacgcag
     2401 tagcctgccg cattcaattc gcacacgccg acgcagggac tgagcacagg ttttttcggg
     2461 gccggggaaa agaacatgga gcactatggg aataaccgat cattcttgct agattcgcca
     2521 ctgctttcac ggcggcgatg cgatgctggc actggcgacc aaagtggtaa ccgactacgg
     2581 ccgcgttctg gcgcaggtgc aaccgggatt gcacggcgtg cccgaatcgc tgctgccgca
     2641 ttcgaaagcg cagatccgcg acgccatcgt catattactc cagcatctgg agcccgaaca
     2701 cac
//
LOCUS       DASRVO010000853         1080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_232957, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000853 DASRVO010000000
VERSION     DASRVO010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232957"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFF05_10600"
     CDS             complement(<1..828)
                     /locus_tag="VFF05_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HZW52303.1"
                     /translation="MSRRHLTQSEREQLELFRALPGDLAPRDAQDLMAYPFFSLAKTH
                     RTTPIDYRMNDIAIRVEAVPEHGMATIWDADVLIWAASQIVKARDADLRTSRLMAATP
                     YEILTFIGRGVSARDYHRLKAALDRLQSTTVATSLRQTSERRMHRFSWINEWTERADA
                     HGRADGIDLIVPDWFYRAVLDDALVLTIDREYFKLTGGLERWLYRIVRKHAGRQRAGW
                     RFDFRHLHGKSASLSPFKRFAFELRDLARRQPLPGYRLGVERDRTGRELLVFARTHLS
                     "
     gene            complement(837..>1080)
                     /locus_tag="VFF05_10605"
     CDS             complement(837..>1080)
                     /locus_tag="VFF05_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZW52304.1"
                     /translation="TPEAARFVGLSIRTLEKHRIYGTGPRYSKLGGRVVYAVEDLQSW
                     VQSAAKASTSDPGKVVVLPARRHTPAELEQARHPRR"
BASE COUNT          180 a          313 c          400 g          187 t
ORIGIN      
        1 ggataagtgg gtgcgggcga agaccagaag ctcgcggcct gtgcggtcgc gttcgacgcc
       61 gagacgatag ccgggcagcg gttgcctgcg cgcgagatcg cggagctcga acgcgaagcg
      121 tttgaacggc gaaaggctcg ccgacttgcc gtggaggtga cggaagtcga accgccaacc
      181 ggcacgctgc ctgcccgcgt gcttgcgtac gatgcgataa agccagcgct cgaggccgcc
      241 ggtcagcttg aaatattcgc ggtcgatggt gagcacgagc gcgtcgtcga ggacggcgcg
      301 gtagaaccag tccggcacga tgagatcgat gccgtcggcg cggccgtgcg catcggcgcg
      361 ttcggtccat tcgttgatcc aggaaaagcg gtgcatgcgc cgctcgctcg tctggcgcag
      421 cgatgtggcg accgtggtgg attgcagccg gtcgagcgcc gccttcaggc ggtgataatc
      481 gcgcgcactg acaccgcggc cgatgaacgt cagaatctcg tagggggtgg cggccatcag
      541 gcgggatgtc cgcaggtcgg cgtcgcgtgc tttgacgatc tggctggcgg cccagatcaa
      601 cacgtcagcg tcccagatgg tcgccatgcc gtgctcgggc acggcttcga cgcggatggc
      661 gatgtcgttc atccggtagt cgatcggcgt cgtgcgatgc gtcttggcga ggctgaagaa
      721 gggataggcc atgaggtctt gcgcgtcgcg cggcgcgaga tcgcctggca gtgcccgaaa
      781 caattcgagc tgctcgcgct ctgattgggt gagatgccgc cgcgacatga gttctgtcag
      841 cggcgtggat ggcgggcttg ttcgagttca gccggcgtat ggcgccgcgc cggcagcacg
      901 acgaccttgc cggggtcgga cgtcgacgct ttggcggcag attggaccca gctctgaaga
      961 tcttcgaccg catagacgac gcggccaccg agcttcgaat agcgcggccc ggtgccgtag
     1021 atgcggtgct tctcaagggt gcggatggac agcccgacga agcgcgcagc ctcgggtgtg
//
LOCUS       DASRVO010000854         3098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_25219, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000854 DASRVO010000000
VERSION     DASRVO010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25219"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1422)
                     /gene="hemL"
                     /locus_tag="VFF05_10610"
     CDS             complement(133..1422)
                     /gene="hemL"
                     /locus_tag="VFF05_10610"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZW52305.1"
                     /translation="MPTNHELFQRAQPRLPGGVNSPVRAFRSVGGEPFFTDHADGAYL
                     WDVEGKRYIDYVGSWGPMIVGHNHPKVRSAVEHAVRAGLSFGTPCPAEVTMAETIARL
                     VPSMQMLRMVNSGTEATMSAIRVARGFTGRDKIVKFEGCYHGHGDSFLVKAGSGALTF
                     GVPTSPGVPKALADLTLTLPYNDLDAAQELFASSGDEIAGVIVEPVAGNMNCIPPRPG
                     YLQALRELCTRHGAVLIFDEVMTGFRVALGGAQERYGITPDMSTFGKIIGGGMPVGAY
                     GGRRDIMEKVAPSGPIYQAGTLSGNPVAMAAGLAMLELIQAPGFYEDLERKATLLVDG
                     LRAVAIEAGVPFSTNRVGAMFGLFFTDAKVETYTQATACDVAAFNRFFHAMLDRGVYL
                     APSAFEAGFLSATHSEQDIADTLDAARAAFKESRHAS"
     gene            complement(1431..2054)
                     /gene="thiE"
                     /locus_tag="VFF05_10615"
     CDS             complement(1431..2054)
                     /gene="thiE"
                     /locus_tag="VFF05_10615"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZW52306.1"
                     /translation="MHMPTFPTRGLYAITDGPRADLVDACAAAMEGGAAVLQYRDKTP
                     DRARRRDEASALAALCARHRVPLIINDDVDLAAVIGAAGVHLGEDDADIAAARSRLGS
                     GAIIGVSCYDSLERARDLAAAGADYLAFGAFFPSPTKPQARRASPDLLRAARAFSKPL
                     VAIGGITPDNATALIAAGADFVAAVSGVFGQSNVADAARHYATLFHK"
     gene            2116..2319
                     /locus_tag="VFF05_10620"
     CDS             2116..2319
                     /locus_tag="VFF05_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZW52307.1"
                     /translation="MNAVLQSDNVPFRSFMCVVCGFIYDEAEGWPADGIAPGTRWEDI
                     PETWTCPDCGVTKSDFEMVEVSR"
     gene            complement(2353..>3098)
                     /locus_tag="VFF05_10625"
     CDS             complement(2353..>3098)
                     /locus_tag="VFF05_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZW52308.1"
                     /translation="EYPDLDSAAYESHLRGYTQRLRELPKGNESAAQLHAINRFLYDE
                     LGFCGDDRDYYDPRNSYLNDVLDRRMGNPISLAVVQIELARRLGVPLEGISFPGHFLV
                     RLPLEEGIVVLDPYQKGRSLDVAELRRRARAHTDAQELDDDHLARMLEPASHRAILTR
                     MLRNLKGVYGEREQWDKAARCCDRLLILDALSVSEYRDRGACYLHLGHVRGAREDWQR
                     YLALMPQADDAEEIALKLAELGSATPRLN"
BASE COUNT          541 a          964 c         1066 g          527 t
ORIGIN      
        1 tcgcgctggt attcgtagcc ggcgatgcgt tcggcagcgg gcccgtgcgc gaacgtggtc
       61 gacaggtgga tcggcggcgc gaccgcgccg gtggcggcgt cgggttccgc accggcatgc
      121 acggcgagcg tttcaggacg catgacgcga ctccttgaac gcggcacgtg cggcatcgag
      181 cgtgtcggca atgtcctgct cgctgtgcgt ggcggaaagg aatccggcct cgaacgccga
      241 cggcgccagg tacacgccgc gatcgagcat ggcatggaag aagcgattga atgcggcgac
      301 gtcgcaggcc gtggcctgtg tatacgtttc cacttttgca tcagtgaaga acaggccgaa
      361 catggcgccg acgcggttcg tgctgaacgg cacgccagcc tcgatcgcga cggcacgcag
      421 gccatcgacg agcagcgtag ccttgcgttc gagatcttcg taaaagcccg gcgcctggat
      481 cagctcgagc atggcgagtc cggccgccat cgcgaccggg ttgccgctga gcgtgccggc
      541 ctgatagatc ggcccgctcg gcgcgacctt ctccatgatg tcgcggcgcc cgccataggc
      601 gccgaccggc atgccgccgc cgatgatctt gccgaaggtg ctcatgtccg gcgtgatgcc
      661 gtagcgttcc tgcgcaccgc cgagcgcgac gcggaatccg gtcatcacct catcgaaaat
      721 cagcacggcg ccgtggcgcg tgcacagttc gcgcagggcc tgcagatagc cgggacgagg
      781 cggaatgcag ttcatgttgc cggcgaccgg ttctacgatc acgccggcga tttcatcccc
      841 cgaacttgcg aagagttcct gcgcggcatc gagatcgttg tacggcaacg tcagcgtgag
      901 atcggccagc gccttgggca cgccgggcga ggtcggtacg ccgaaggtga gtgcaccgga
      961 gccggccttg accaggaagg aatcgccgtg gccgtggtag cagccttcga atttcacgat
     1021 cttgtcgcgg ccggtgaagc cgcgcgcgac gcggatcgcc gacatcgtcg cctcggtgcc
     1081 ggaattgacc atgcgcagca tctgcatgga tggaaccagg cgcgcgatcg tttcggccat
     1141 cgtcacttcg gccgggcagg gcgtgccgaa ggacagtccg gcgcgcacgg cgtgctcgac
     1201 cgcgctgcgc accttcggat ggttgtgacc gacgatcatc ggtccccatg agccgacgta
     1261 gtcgatgtag cgtttgccct cgacatccca tagatacgcg ccgtcggcat ggtcggtgaa
     1321 aaatggttcg ccgccgaccg agcggaacgc gcgcaccggc gaattgacgc cgccgggcag
     1381 ccggggctgc gcgcgctgga agagttcgtg gttagtgggc atgtgggtgt ctatttgtgg
     1441 aaaagcgtgg cgtagtggcg tgccgcgtcc gcgacattag actggccgaa tacgccggaa
     1501 accgccgcga caaaatcggc gccggccgca atcaacgccg tcgcattgtc cggcgtaatg
     1561 ccgccgatcg ccaccagcgg cttgctgaat gcgcgcgcgg cgcgcaacaa atccgggctg
     1621 gcgcggcgtg cctgtggctt ggtcggcgat gggaagaatg cgccgaaggc aaggtaatcc
     1681 gcgcccgctg cggcaagatc gcgagcacgt tccagcgagt cgtagcagga gacgccgatg
     1741 atggcgccgc tgccgagccg gcttcgcgcg gcggcaatgt ccgcatcgtc ctcgcccaag
     1801 tgcacgccgg ccgcgccgat cacggcggcg aggtcgacat cgtcgttgat gatcagcggc
     1861 acgcgatgcc gagcgcacag agcggcgagc gcgcttgctt cgtcgcgacg acgtgcgcgg
     1921 tccggagtct tgtcgcggta ttgcaggacc gcggcaccgc cttccatggc ggcagcgcag
     1981 gcatcgacga gatcggcgcg cggtccatcg gtgatggcgt aaagtccacg cgtgggaaat
     2041 gtgggcatgt gcatgaccgc aagtgtaacg ggtagaataa gcgcccgaaa ttcctgcttc
     2101 gccccgatat tttcgatgaa cgccgtactt caatccgaca acgtgccatt ccgcagcttc
     2161 atgtgcgtgg tctgtggctt catctacgac gaggcggagg gctggcccgc ggacggcatc
     2221 gcgccgggca cgcgctggga agacattccg gaaacatgga cctgtcccga ctgcggcgtg
     2281 accaagtcgg atttcgagat ggtcgaggtg tcgcgctgac gcacgcgcct ggcgacagcc
     2341 gggcaggcga ggtcagttga gcctgggcgt tgcggagccc aactccgcca gcttcaacgc
     2401 gatttcctcg gcatcatcgg cctgcggcat caacgcgaga tagcgctgcc aatcctcgcg
     2461 cgcgccgcgc acgtgaccca gatgcaggta gcacgcgccg cgatcgcggt attcggatac
     2521 cgacagggca tcgaggatga gcaggcggtc gcagcaacgc gcggccttgt cccactgctc
     2581 gcgttcgccg tagacgccct tcaggttgcg cagcatgcgc gtgaggatcg cgcgatggct
     2641 ggccggttcg agcatccgcg ccaaatggtc gtcgtcgagt tcctgcgcgt cggtatgcgc
     2701 acgcgcgcgg cgacgcaact cggcgacatc gagcgagcgg cctttctggt agggatcgag
     2761 caccacgatt ccctcttcca gcggcaaacg cacgaggaag tgcccgggaa acgagatgcc
     2821 ttcgagtgga acgcccaggc gccgcgccaa ctcgatctgc accaccgcga gcgagatcgg
     2881 attgcccatg cggcggtcaa ggacgtcgtt cagatagctg ttgcgcggat cgtagtaatc
     2941 gcgatcgtcg ccgcagaatc caagttcgtc gtacagaaaa cggttgattg cgtgcaattg
     3001 cgccgcactc tcgttgccct tgggcagttc gcgcagacgt tgcgtgtagc cacgcaggtg
     3061 cgattcgtag gcggcggagt cgaggtcggg gtattcgt
//
LOCUS       DASRVO010000855         1209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_388336, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000855 DASRVO010000000
VERSION     DASRVO010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388336"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..134
                     /locus_tag="VFF05_10630"
     CDS             9..134
                     /locus_tag="VFF05_10630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HZW52309.1"
                     /translation="MAKAYLDMGDPEGARSMLDEVLAEGNDAQKVEARKLLAEIG"
     gene            368..1138
                     /gene="truA"
                     /locus_tag="VFF05_10635"
     CDS             368..1138
                     /gene="truA"
                     /locus_tag="VFF05_10635"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZW52310.1"
                     /translation="MRIALGIEYDGTDFLGWQRLSHGSTVQGAVETALTDIAAEPVQV
                     VCAGRTDAGVHARNQVIHFDTQAQRTMRGWTLGTNSRLPRSVAVRWARPVSDEFHARF
                     SARARRYRYTILNRSARPALEARYVCWERAPLDAAAMHAAAQYLVGEQDFSAFRTVAC
                     QARSPMRCVQAIGVAREGDRVVLDIQANAFLHHMVRNIAGSLRVVGRGEQPPEWIVQL
                     LAGRDRSVAGPTSAAQGLVFIGPLYPQQFGLPAEATLP"
BASE COUNT          202 a          393 c          385 g          229 t
ORIGIN      
        1 agctcgatct ggccaaggcc tacctcgaca tgggcgatcc ggaaggcgcg cgctcgatgc
       61 tcgacgaagt gctggccgaa ggcaacgacg cgcaaaaggt cgaagcgcgc aagctgctgg
      121 cggaaatcgg ataacgcatt cacgtctctc cgacgtacga tgttggccgg gcttgtcccg
      181 gcctcttttt ttgaagcggt gcagaaactg ctgcgctcga cagctcgcgt cccggcggtg
      241 ctcggattcc tcatttacag tcaagtaaac tccggatcct gcgctccggc ggaacgcaat
      301 ctgtcttcgc tcgcgacgtt tcatgctacc gcttcaacct gcttcgtgaa ttcattggca
      361 tacctgaatg cgcatcgcgc tcggcatcga atacgacggc accgatttcc tcggctggca
      421 gcgcctgtcg catggatcga cggtgcaggg cgcggtcgaa acggccctga cggacatcgc
      481 cgccgagccc gtacaggtcg tctgtgcggg gcgcaccgat gccggcgtgc atgcgcgtaa
      541 tcaggtgatc cacttcgaca cgcaggcgca acgcacgatg cgcggctgga cgctcggcac
      601 caattcgagg cttccgcgta gcgttgcagt gcgttgggcg cggccggtca gcgatgaatt
      661 ccatgcgcgc ttctccgcgc gcgcgcgacg ctaccgctac accatcctga accgctcagc
      721 gcgtccggcg ctggaggcgc gttatgtgtg ctgggaacgc gcgccgctgg acgcagccgc
      781 aatgcacgcg gcggcgcaat atctggtcgg tgaacaggat ttttccgctt ttcgtaccgt
      841 cgcctgccag gcgcgctcgc cgatgcgctg cgtgcaggcc attggcgtcg cgcgcgaggg
      901 cgatcgcgtc gtgctcgaca tccaggccaa cgcattcctg catcacatgg tgcgcaatat
      961 cgccggcagc ctgcgcgtgg tcgggcgcgg cgaacaacca ccagaatgga tcgtgcagct
     1021 tctcgccggg cgcgaccgaa gcgtggccgg cccgacctcg gccgcgcaag gtctggtctt
     1081 tatcggaccg ttgtatcctc agcagttcgg acttcccgcc gaggcgacgc tgccgtgaac
     1141 cgcgtgcgta tcaaattctg tggcattacc cgcgtgcaag acgcgacgat cgcgtgcgcg
     1201 cttggcgtc
//
LOCUS       DASRVO010000856         1156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_337312, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000856 DASRVO010000000
VERSION     DASRVO010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337312"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="miaA"
                     /locus_tag="VFF05_10640"
     CDS             complement(<1..947)
                     /gene="miaA"
                     /locus_tag="VFF05_10640"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZW52311.1"
                     /translation="MSADQRPPAIFLMGPTASGKTALACALAECFSFAIVSVDSALVY
                     RGLDIGAAKPDAATLAKYPHRLIDIRDPAQPYSAAEFRADALREMAAITASGKIPLLV
                     GGTGLYFRALQAGLSDLPEADPSLRTKLAEEARVLGWSAMHERLHEVDPAAASKIRPG
                     DAQRIQRALEVIELTGKPLSAQQGGMPARFGYRVLKLALIPDDRAQLHERIAQRMDAM
                     IDAGFLDEVRGLRGRGDLQPDLPALRAVGYRQAWQHLDGAFGADEFRDRAVFATRQLA
                     KRQLTWLRSELDARRLDPLIVDHAAQAQRAVTEFLTGPSP"
BASE COUNT          208 a          390 c          363 g          195 t
ORIGIN      
        1 ggcgaaggcc cggtaaggaa ttccgttacc gcgcgttgcg cctgcgcggc gtggtccacg
       61 atgagcggat cgagtcttcg cgcatcaagc tccgagcgca gccaggtgag ctggcgcttg
      121 gccagttggc gcgtggcgaa caccgcgcgg tcgcggaatt cgtccgcgcc gaatgcaccg
      181 tccagatgtt gccaggcctg ccggtagccg actgcccgca gcgccggcag atcgggctgc
      241 aaatcgccgc gcccgcgcaa gccacgaact tcatcgagga agcctgcatc gatcatggcg
      301 tccatgcgct gcgcgatgcg ttcgtgcaat tgcgcgcgat catccggaat cagcgcgagc
      361 ttgagcacgc gataaccgaa ccgcgccggc atcccgccct gctgcgccga caacggcttt
      421 ccagtcagtt cgatcacctc cagcgcgcgc tggatgcgct gcgcgtcgcc ggggcggatt
      481 ttcgacgccg ccgctggatc gacttcatgc aggcgttcat gcatcgccga ccagccgagt
      541 acgcgggctt cttccgccaa tttcgtgcgc aaggacggat cggcttccgg cagatccgaa
      601 agccccgctt gcagcgcgcg gaaatacaag ccggtgccgc caaccagcaa cgggatcttt
      661 cccgatgccg tgatcgccgc catttcacgc agcgcgtcgg cacggaactc cgccgccgaa
      721 tagggttgtg ccggatcacg gatatcgatc aaccgatgcg gatacttcgc cagcgtcgcc
      781 gcatcgggtt tggccgcgcc gatgtccagt ccacgataga ccagcgccga atccacgctg
      841 acgatggcga acgaaaaaca ctccgccagc gcgcaggcca gcgccgtctt gcccgacgcg
      901 gtcggaccca tcaggaaaat cgcgggcggg cgctgatcgg cagacatcgg caaagtgtaa
      961 gggctttgcc gcatgtgcgc cgcaggcccg ccgcgacggg cgaggacgga aaccgctcag
     1021 ttgcgtttgc cgaccaacag gatgccacca atgacgatcg cgccaatgcc ggcccagacc
     1081 ggaatgtcga cggtcttctt ctccttcacg ttgacctcta tcgggccgag tttggcctcg
     1141 tggtcttcct gggtat
//
LOCUS       DASRVO010000857         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_361913, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000857 DASRVO010000000
VERSION     DASRVO010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361913"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1080)
                     /locus_tag="VFF05_10645"
     CDS             complement(70..1080)
                     /locus_tag="VFF05_10645"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HZW52312.1"
                     /translation="MKSVSLTRFLIEEQRDKGHINSDLRLLIEVVARACKSISIAVGR
                     GALGGVLGSAGTENVQGEVQKKLDVLSNEILLEANEWGGHLAALASEEMDDPHPIPHR
                     YPKGEYLLVFDPLDGSSNIDVNISVGTIFSVLRCPAGVKEPDEKAFLQPGTQQVAAGY
                     AVYGSSTMLVLTLGDGVHAFTLDREIGSFVLTQRDLRIPEDTREFAINMSNLRHWEAP
                     MRRYIEELLAGKSGPRGADFNMRWVASMVADVHRIVCRGGVFIYPLDAKIRDKGGKLR
                     LMYEANPMAFIVEQAGGMATDGHGRILDIVPQSLHQRVPVFLGSKNEIERVTQYHREE
                     PR"
     gene            complement(1159..1572)
                     /locus_tag="VFF05_10650"
     CDS             complement(1159..1572)
                     /locus_tag="VFF05_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52313.1"
                     /translation="MPASTKLLVVVGADDHRAYESLGLSFYADTIRVPAANKNVIELE
                     SDDHGSPPLLANHAAPGASLDAHPPRRALIHEFEHAGVVDALDWYGDWKLFDALSDCA
                     FHGRECATALGGGPAQIDMGAWSDGVPVRPMRVLR"
     gene            complement(1618..2175)
                     /locus_tag="VFF05_10655"
     CDS             complement(1618..2175)
                     /locus_tag="VFF05_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZW52314.1"
                     /translation="MISPLRPILFVVLLAATALAHAVAPTPPVQPAHGPGGAGYAHAQ
                     VLAREVKSGAQGWWLFVPSSPKPASAPIVVFCHGWGALDPKGYRAWIDHIVRRGNIVI
                     WPNYQASLLTPGGDFLPNAIVGIRAALADLENGSAGICPELDHVAIVGHSAGGVLSAQ
                     IAAAAARNDLPVFRAVVPVEPGDGS"
     gene            2311..>2758
                     /locus_tag="VFF05_10660"
     CDS             2311..>2758
                     /locus_tag="VFF05_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HZW52315.1"
                     /translation="MKFHALVSLALGLALTIPAWADAPDCACAKATAPAPGSADAKFD
                     AIYSAEWKWRQAQFPGGDDDSVNAAVPDRLPRVDAAAQDARLKFWRDVLAKLATIPVD
                     KLSPANRINYAVYKPQIADLAADVRFRQYEMPFNADSQFWSDLGFVS"
BASE COUNT          467 a          874 c          923 g          494 t
ORIGIN      
        1 ctcgccgttt cgtaccccag cattcgcacc ggatgtagcg gcctggcgtt gcgccaggag
       61 ctgttttgct cagcggggtt cttcgcggtg atactgggtc acgcgctcga tctcgttctt
      121 cgagccgagg aacaccggca cgcgctgatg cagcgattgc ggcacgatgt cgagaatgcg
      181 gccgtggccg tcggtggcca tgccaccggc ctgctcgacg atgaaggcca tcggattggc
      241 ctcgtacatc aggcgcagct tgccgccctt gtcgcggatc ttcgcatcca gcgggtagat
      301 gaagacgccg ccgcggcaga cgatgcggtg tacgtcggcc accatgcttg ccacccagcg
      361 catgttgaaa tccgcaccgc gcggaccgct cttgccggcg agcagttcct cgatgtagcg
      421 ccgcatcggc gcttcccagt ggcgcaggtt cgacatgttg atggcgaact cgcgtgtgtc
      481 ctccgggata cgcaggtcgc gctgggtcag cacgaagctg ccgatctcgc ggtcgagcgt
      541 gaacgcgtgc acgccgtcgc cgagcgtgag caccagcatg gtgctcgaac cgtacaccgc
      601 gtagccggcg gcgacttgct gcgtgcccgg ttgcaggaac gccttttcgt cgggctcctt
      661 cacgccggcc ggacagcgta gcaccgagaa gatggtgccg acggagatgt tcacgtcgat
      721 gttcgaggag ccgtccagcg gatcgaacac gagcaggtat tcgcccttgg gatagcggtg
      781 cgggatcgga tgcggatcgt ccatttcctc ggatgccagc gcggccagat gcccgcccca
      841 ttcattggct tcgagcagga tctcattcga cagcacgtcg agttttttct gcacctcgcc
      901 ctgtacattt tcggtccccg cgctgccgag cacgccacca agcgcgccgc ggccgacggc
      961 aatggaaatc gatttgcagg cgcgcgcgac cacctcgatg agcaggcgca ggtcggaatt
     1021 gatgtgcccc ttgtcgcgct gctcctcgat caggaagcgg gtcaaggaga cgctcttgat
     1081 cgactcaggc ttgttcgacg acggtgagtt catgacgcaa accggactat gcggtccgcg
     1141 cattgtcgca tgcgtccttc agcgcagcac gcgcatcggc ctcaccggca cgccatcgct
     1201 ccacgcgccc atgtcgattt gcgcgggacc tccgccgagg gcggtcgcgc attcgcggcc
     1261 gtggaaggcg cagtcgctta gcgcatcgaa caacttccaa tcgccgtacc agtccagtgc
     1321 atcgaccacg ccggcgtgct cgaactcgtg aatgagcgcg cgccgcggcg gatgggcatc
     1381 gagcgacgcg ccgggcgcgg catggttggc cagcagcggc ggggaaccgt gatcgtcgct
     1441 ttccagctcg atcacgttct tgttcgcggc gggcacgcgg atcgtgtcgg catagaagct
     1501 caggccgagc gattcgtagg cgcgatgatc gtccgcgccg acgacgacga gcaacttggt
     1561 cgatgcgggc atcggtgaaa gatcggcgtg cggcacggtt acgcgcttgc gcccgtccta
     1621 gctgccgtcg ccgggctcga ccggcaccac ggcgcgaaac acgggcaagt cgttgcgtgc
     1681 cgcggcggcg gcgatctgcg cgctgagcac accaccggcg gagtggccaa cgatggcgac
     1741 atggtcaagc tcagggcaga ttcccgccga gccgttttcc agatcggcca gcgcggcgcg
     1801 aataccgaca atggcgttcg gcaggaagtc gccgcccggc gtgagcagac tggcctggta
     1861 attcggccag atcacgatgt tgccgcggcg cacgatgtgg tcgatccagg cgcgatagcc
     1921 cttgggatcg agcgcgcccc agccgtggca gaagacaacg atcggcgcgc tggcgggctt
     1981 tggcgaggat ggcacgaaca gccaccagcc ctgcgcgccg cttttcactt cgcgcgcgag
     2041 gacctgcgcg tgcgcgtagc cggccccgcc ggggccgtgt gcgggttgca cgggtggtgt
     2101 gggtgcgacg gcatgcgcga gtgcagtggc ggcaagcagg acgacgaaaa gaatcggtcg
     2161 caatggcgaa atcatgggaa ctccttgtcg attgaccgtt tctttgcgtc aacgcactgg
     2221 cggcgattgc gtggacatgc cggcagcaac ggccggtgca tggcgaagcc caagatgcga
     2281 tgatcttgga tcgtccgcaa ggagatcgtc atgaaattcc acgcactcgt gtcgctcgcg
     2341 ctcggtctgg cgctgacgat tcccgcctgg gcggatgcgc ccgattgcgc ttgcgcgaaa
     2401 gccaccgcac ccgcgccggg atcggccgat gcgaagttcg atgcgatcta cagcgccgaa
     2461 tggaaatggc ggcaggcgca gtttccgggc ggtgatgacg acagcgtaaa cgcagcggtt
     2521 cccgatcgtc tgccgcgcgt ggatgcggcc gcgcaggatg cgcgcctgaa attctggcgc
     2581 gacgtgttgg cgaagctcgc aacgattccc gtcgacaagc tctcgcccgc caaccgcatc
     2641 aattacgctg tctacaaacc gcagatcgcc gatcttgccg cggacgtgcg atttcgccag
     2701 tacgagatgc cgttcaacgc cgattcgcag ttctggtcgg atctgggctt cgtgtccg
//
LOCUS       DASRVO010000858         1450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_25351, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000858 DASRVO010000000
VERSION     DASRVO010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25351"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VFF05_10665"
     CDS             complement(<1..564)
                     /locus_tag="VFF05_10665"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HZW52316.1"
                     /translation="MLRLSSASNFRHVGGYAAHDGRRVAANRIFRSDHLAGLTPEEAR
                     QVSALGLTRILDLRGVTERAAAPCALRAATVHSLPIEPTIVRKIAELIAAGQTLSRAD
                     VVALMQDTYRGFVFHNTHRFAELFAYLLESDEPTVFHCTAGKDRTGFAAALVLRSLGV
                     REEDVMRDYLLTNERLKPRVPSRADLAP"
     gene            complement(606..>1450)
                     /locus_tag="VFF05_10670"
     CDS             complement(606..>1450)
                     /locus_tag="VFF05_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HZW52317.1"
                     /translation="PVEGVVRRRGCDGLRTVAQGMSYMQPQTQPTESTSHTSRVAVWS
                     SAWCAGRTTDQGDIYGSAIFQIVLFEPRQLQPRPGGQQGRQSQHAKQPGAERARQRQQ
                     QREQAQQQQQRKKLVAQQVRLAFPRLTREAALHTMPGRQQRPGILLQAQSAQATGLAK
                     ANRMDGALESLTAALRQFAAEREWGQFHSPKNLAAALTVEAAELLEHFQWLTERQSSE
                     LPAPAREAVAQEMADVLLYLLQLADKLNVDLVDAAQRKLALNARKYPVASSRGHSRKH
                     DGRS"
BASE COUNT          202 a          487 c          486 g          275 t
ORIGIN      
        1 gggcgccagg tcggcgcgcg acggcacgcg cggcttgagg cgctcgttgg tcagcaggta
       61 gtcgcgcatc acgtcctctt cccgcacgcc gaggctgcgc agcacgagcg ccgccgcgaa
      121 tccggttcgg tccttgcccg ccgtgcagtg gaagacggtg ggctcgtccg attccagcag
      181 atacgcaaac agttcggcga accgatgcgt gttgtggaac acgaagccgc ggtaggtgtc
      241 ctgcatcaac gcgaccacgt cggcgcgcga cagcgtttgg cccgcggcaa tcagctccgc
      301 gatcttccgc acgatggtgg gttcgatcgg cagcgagtgc accgtcgccg cgcgcagggc
      361 gcacggcgcg gccgcgcgct cggtcacgcc acgcaaatcg aggatgcgcg tgagtccgag
      421 cgccgagact tgccgggctt cttcgggcgt gaggccggcg agatggtccg aacggaagat
      481 gcggttcgct gcgacccgcc ggccgtcatg cgcggcatac ccgccgacgt ggcggaaatt
      541 cgaggcactg gaaagtctca acatgatcga tcggatcagg gccgcgccgg gcgcgcgccg
      601 cgccgctagg accggccgtc gtgtttcctg ctgtggccgc gcgagcttgc caccggatac
      661 ttccgcgcgt tcaacgcgag cttgcgttgt gcggcgtcga ccaggtccac gttcaatttg
      721 tcggccagtt gcagcaggta cagcagcacg tcggccattt cctgcgccac cgcctcacgc
      781 gcgggtgccg gcagctcgct gctctgccgt tcggtcagcc actggaaatg ctcgagcaat
      841 tccgcggctt cgaccgtcaa cgccgccgca agattcttcg gcgagtggaa ctggccccac
      901 tcccgctcgg cggcgaactg ccgcagtgcc gccgtcagcg attccagcgc tccgtccatc
      961 cgatttgcct tcgcgagtcc cgttgcctgc gccgattgtg cctgcaaaag aatgcccggc
     1021 cgctgttgcc ggccgggcat cgtgtgcaag gctgcctccc gggtcagcct ggggaaggca
     1081 agccttactt gctgcgcgac gagctttttt cgctgctgct gctgctgcgc ttgctcgcgt
     1141 tgctgttgcc gctggcgcgc tcgctctgcg cccggctgtt ttgcgtgctg gctttgccgc
     1201 ccttgctggc cgcctgggcg cggctggagc tgccgcggct cgaagaggac gatttggaag
     1261 atcgcgctgc catagatatc tccttggtct gttgtgcggc cggcgcacca tgcgcttgac
     1321 cacaccgcga ctctagaagt gtgggaagtg ctttctgtcg gctgtgtttg cggttgcatg
     1381 taggacatcc cttgtgcgac ggttcgcagc ccgtcgcacc cacggcgccg cacgacacct
     1441 tcgacgggcc
//
LOCUS       DASRVO010000859         1423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_233214, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000859 DASRVO010000000
VERSION     DASRVO010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233214"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFF05_10675"
     CDS             complement(<1..192)
                     /locus_tag="VFF05_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52318.1"
                     /translation="MLNAYPAFFNLLTLIRYYRGLPLQKRTVPMRFPTLSRLGLMLFA
                     VLLASCHRGEPKKDAAEYLP"
     gene            191..1159
                     /gene="rluD"
                     /locus_tag="VFF05_10680"
     CDS             191..1159
                     /gene="rluD"
                     /locus_tag="VFF05_10680"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019304358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HZW52319.1"
                     /translation="MDQDPATRRTHLPPEAAGRRLDQALAELFPEFSRSRLTAWIKSG
                     DVRVDGATAIPRQIVRGGEEVILRAELAPEVAMAPEAIALDIRYEDADVIVVNKPAGL
                     TVHPGAGQPSGTLQNALLHHDPELAKIPRAGIVHRLDKDTSGVMVVARSLRAHTALVE
                     QLQARAMHRQYVAVVYGTMIAGGSVDAPIGRHPRDRLKQAIVEEPGGKPAVTHYRVRE
                     RFRAHTLIECRLETGRTHQIRVHMAHVRHALVGDPVYGAGLKLPKGATPELAEAMRGF
                     RRQALHAESLEFTHPADGRAIRVEAQLPQDMQALIAALRADSKEHG"
     gene            1200..>1423
                     /locus_tag="VFF05_10685"
     CDS             1200..>1423
                     /locus_tag="VFF05_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017916183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HZW52320.1"
                     /translation="MPDWPAPSRVHALATTRRLPGNSQPPYDAFNLGLRSGEDASTVH
                     ANRALLQRAFALPAPPRWLHQVHGDRVLRMT"
BASE COUNT          249 a          485 c          479 g          210 t
ORIGIN      
        1 gggcaggtac tcggcggcgt ccttcttcgg ctcgccgcga tggcaggagg caagcaggac
       61 ggcgaacagc atcaggccaa gcctggacaa ggtcggaaat cgcatgggga cggtacgctt
      121 ttgcaagggc aaaccgcgat aataccggat aagcgttaga agattgaaaa acgcaggata
      181 ggcattcagc atggatcagg atcccgcaac gcgccgcacc cacctgccgc ccgaggccgc
      241 cggtcggcgc ctggaccagg cgctggccga gctgttcccg gaattttcgc gctcgcgtct
      301 gaccgcgtgg atcaagtccg gcgacgtgcg cgtggacggc gcgacagcga ttccgcgaca
      361 aatcgtgcgc ggcggcgaag aagtgatcct gcgcgccgaa ctcgcgccgg aagtggcgat
      421 ggcgccggaa gcgatcgcgc tggacatccg ctacgaggat gccgatgtca tcgtcgtgaa
      481 caagccggcg ggcctgaccg tgcatccggg cgccgggcag ccgtccggaa ccctgcagaa
      541 cgcgttgctg caccacgatc cggaactggc gaagattccg cgcgccggca tcgtgcaccg
      601 gctcgacaag gatacctcgg gcgtgatggt cgtcgcgcgc tccctgcgcg cacacaccgc
      661 gctggtcgag cagttgcagg cgcgcgccat gcaccgtcaa tacgtcgcgg tcgtttacgg
      721 aacgatgatc gccggcggca gcgtggacgc gccgatcggt cgccatccgc gcgaccggct
      781 gaaacaggcc atcgtcgagg aacctggcgg caaacccgcg gtcacccatt atcgggtgcg
      841 cgaacgtttc cgcgcgcata cgctcatcga atgccgcctg gaaaccgggc gcacccacca
      901 gattcgcgtg cacatggcgc atgtgcgcca tgcgctggtc ggcgacccgg tttacggcgc
      961 cggcctgaag ttgcccaagg gcgcgacgcc cgagctggcc gaagcgatgc gcggtttccg
     1021 gcgccaggcc ctgcacgccg aaagcctgga gttcacgcat ccggccgacg gccgcgcgat
     1081 ccgcgtcgag gcgcaattgc cgcaggacat gcaggcgctg atcgcggcgc tgcgcgcgga
     1141 ttcgaaggaa catggctgag gcccgattta cgtcggtcgc gatcgcggcg tccgtcatcg
     1201 tgccggattg gcccgcacca tcgcgcgtgc atgcgctggc caccacgcgc cggttgcccg
     1261 gcaactcgca gccgccctac gacgccttca atctcggcct gcgcagcggt gaggacgcat
     1321 ccaccgtgca cgccaaccgt gcgctgctgc aacgcgcctt tgccttgccc gcgccaccgc
     1381 gctggctgca tcaggtgcac ggcgatcgcg tcctgcgcat gac
//
LOCUS       DASRVO010000860         1385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_180014, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000860 DASRVO010000000
VERSION     DASRVO010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180014"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VFF05_10690"
     CDS             complement(<1..1078)
                     /locus_tag="VFF05_10690"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HZW52321.1"
                     /translation="MNAPSRTDALDVAAVRRDFPILSRTVYDKPLVYLDNAASAQKPQ
                     AVIDAVAAAYGAEYANVHRGLHFLSNLATEKYEEAREIVRRFVNAASTDEIIFTRNAT
                     EAMNLVAASFGGSAIGNGDEIVLSIMEHHSNIVPWNFLRERRGAVIKWAPVAGDGTFL
                     IEEFEKLLSPRTKMVAITQMSNALGTAVPVKEVVRLAHERGIPVLVDGAQGAVHLDVD
                     VRDIDCDFYVMTGHKLYGPTGIGVLYGKYAHLATMPPFNGGGEMIQEVAQDWITYGEP
                     PYRFEAGTPAIVQAIGLGAAIDYVNSIGKARIRAHEGALVKYAHERLGAINSLRIIGN
                     AKDKGPIVSFEIKGAHPHDVATIID"
     gene            complement(1075..>1385)
                     /locus_tag="VFF05_10695"
     CDS             complement(1075..>1385)
                     /locus_tag="VFF05_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HZW52322.1"
                     /translation="AQKTDAKMMAQALLLSEQAQFASKPELEIFADDVQCGHGATSGR
                     IDETQLFYLLARGVPRAEAEQLLIEAFLDDAIDTLKDETIAASLKGVVSAWLRKRGSL
                     "
BASE COUNT          228 a          453 c          453 g          251 t
ORIGIN      
        1 ggtcgatgat ggtggcgaca tcgtgcggat gcgcgccctt gatctcgaag gagacgatcg
       61 ggcccttgtc cttggcattg ccgataatgc gcagcgaatt gatggcgccg agccgctcat
      121 gcgcgtattt gaccagcgcg ccttcgtgcg cgcggatgcg cgccttgccg atggaattga
      181 cgtagtcgat ggccgcgccc agcccgatcg cctgcacgat cgccggcgtg ccggcctcga
      241 acctgtaggg cggctcgccg taggtgatcc agtcctgtgc gacttcttgg atcatctcgc
      301 cgccgccgtt gaacggcggc atggtggcga gatgcgcata tttgccgtag agcacgccga
      361 tgccggtcgg cccgtacaac ttgtggccgg tcatcacgta gaaatcgcag tcgatgtcgc
      421 gcacgtcgac gtcgagatgc accgcgccct gcgcgccgtc gaccagcacc gggatgccgc
      481 gctcatgcgc cagccgcacc acctctttca ccggcaccgc ggtgccgagc gcattcgaca
      541 tctgcgtgat cgccaccatc ttggtgcgcg gcgagagcag cttctcgaac tcctcgatca
      601 gaaatgtgcc gtcgccggcg accggcgccc acttgatcac tgcgccgcgc cgctcgcgca
      661 ggaagttcca cggcacgatg ttggagtggt gctccatgat cgacagaacg atctcatcgc
      721 cgttgccgat ggcggaaccg ccgaacgagg cggccacgag gttcatcgcc tcggtggcgt
      781 tacgggtgaa gatgatctcg tcggtcgaag cggcgttgac gaagcggcga acgatctcgc
      841 gcgcctcctc gtacttctca gtcgccaggt tcgagaggaa gtgcaggccg cgatggacgt
      901 tggcgtattc cgccccgtag gcggccgcga cggcatcgat cacggcctgc ggcttctgcg
      961 ccgacgcggc attgtcgaga tagaccagcg gcttgtcgta gaccgtgcgc gacaggatcg
     1021 ggaagtcccg gcgcaccgcc gcgacgtcga gggcatcggt ccggctcggg gcattcataa
     1081 cgagccccgc ttccgcagcc aggcgctgac cacacccttg agcgaagccg caatcgtctc
     1141 gtccttgagc gtatcgatgg cgtcgtcgag gaatgcctcg atcaggagtt gctcggcctc
     1201 cgcccgcggc acgccgcgcg ccaggagata gaagagctgc gtctcgtcaa tgcggccgga
     1261 ggtggcgccg tgcccgcact ggacgtcatc ggcgaaaatc tccagctcag gctttgaggc
     1321 gaactgagcc tgctcggaca gcagcaacgc ctgcgccatc atcttggcgt cggtcttctg
     1381 cgcgt
//
LOCUS       DASRVO010000861         1422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_233373, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000861 DASRVO010000000
VERSION     DASRVO010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233373"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VFF05_10700"
     CDS             <1..226
                     /locus_tag="VFF05_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52323.1"
                     /translation="IPARLITTAGSLGPSYSDFGATVKYQFSTHWNVSVGATYATNKS
                     FYEHTVSFLDPTETKDVGGNRGFVMLQGNL"
     gene            236..808
                     /gene="kdpC"
                     /locus_tag="VFF05_10705"
     CDS             236..808
                     /gene="kdpC"
                     /locus_tag="VFF05_10705"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZW52324.1"
                     /translation="MIKMIRQAIVMLLLLTLITGVIYPLLVTGIAQVAFPRTANGSVI
                     EKDGRPLGSALIGQAFTDPKYFWPRPSTTSPFPDNPSSSGASNLGPTNPALTDAVKQR
                     IAALHAADPGNTAPVPVDLVTASASGVDPDISPAAARYQVARVARARGMPVDAVTRLV
                     ERATAGRQFGILGEPRVNVLQLNLMLDRRW"
     gene            839..>1422
                     /locus_tag="VFF05_10710"
     CDS             839..>1422
                     /locus_tag="VFF05_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973809.1"
                     /note="sensory histidine kinase in two-component
                     regulatory system with KdpE; signal sensing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HZW52325.1"
                     /translation="MSANASAERPDPDALLARLHEDDTRARRGRLKIFFGAAPGVGKT
                     YAMLEAARALKAAGTDVVVGYVEPHGRPETEALLGGLEILPPQELEHRGIRLNEFDLD
                     AALSRHPALLVVDELAHTNHPGARHPKRWMDVQELLADGIDVFTTVNVQHVESVNDVV
                     AQITGVQVRETVPDKVFDGADEVELVDLPPDELL"
BASE COUNT          250 a          495 c          451 g          226 t
ORIGIN      
        1 catcccggcg cgcctgatca cgaccgcagg ttcgctcggt ccgagctaca gcgacttcgg
       61 cgcgacggtg aagtaccagt tctccacgca ctggaacgtg agcgtcggcg cgacctacgc
      121 cacgaacaag tcgttctacg agcacaccgt gagcttcctc gacccgaccg aaaccaagga
      181 tgtcggcggg aaccgcggct tcgtcatgct gcagggaaat ctctgagggt tggccatgat
      241 caaaatgatc cgacaagcaa tcgtcatgct cttgctgctg acgctcatca ccggcgtcat
      301 ctacccgttg ctggtgaccg gcatcgcgca ggtcgcattt ccacgtacgg cgaacggcag
      361 cgtgatcgaa aaggacggac ggccgctcgg ttcggcgctg atcggccagg cgttcaccga
      421 tccaaagtac ttctggccgc ggccctcgac gacgtcgccg tttcccgaca acccgtcgtc
      481 gtccggcgca tcgaacctgg gaccgaccaa tccggcgctg accgatgcgg tcaagcagcg
      541 catcgccgcg ctgcatgccg ccgatccggg caacaccgcg cccgtgccgg tcgatctcgt
      601 caccgcgtcg gcttcgggcg tggatcccga catctcgccg gcggcggcca ggtaccaggt
      661 cgcgcgcgtg gcgcgtgcgc gcgggatgcc ggtcgacgcc gtgacccggc tcgtcgagcg
      721 tgccaccgca ggtcgccagt ttggaatcct gggcgagccg cgcgtcaacg tgctgcaact
      781 caacctgatg ctcgaccgca ggtggtaagc gcggcgcgag ttgcggcatg atgccgccat
      841 gtcggcgaat gcttccgcag aacgccccga tcccgacgcg ttgctcgcgc gcctgcacga
      901 ggacgacacg cgcgcgcggc gcgggcgcct gaagattttc tttggcgcgg ccccgggcgt
      961 cggcaagacc tacgccatgc tcgaagcggc gcgcgcgctc aaggccgccg gcaccgatgt
     1021 agtggtcggc tacgtcgaac cgcacggccg gcccgagacc gaggcgctgc tcggcggcct
     1081 ggagatcctg ccgccgcaag aactcgaaca ccgcggcatc cgcctgaacg aattcgacct
     1141 ggatgccgcg ctgagccgcc atcctgccct tctcgtcgtc gacgaactgg cccacaccaa
     1201 tcatcccggc gcgcgtcatc cgaagcgctg gatggacgtg caggaattgc tcgccgacgg
     1261 catcgacgta ttcaccacgg tcaacgtgca gcacgtggaa agcgtcaacg acgtggtcgc
     1321 gcagatcact ggcgttcagg tgcgcgaaac cgtccccgac aaggtattcg atggtgccga
     1381 cgaggtcgaa ctcgtcgacc tgccgcccga cgaactgctg ca
//
LOCUS       DASRVO010000862         6300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_388692, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000862 DASRVO010000000
VERSION     DASRVO010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388692"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..4771
                     /locus_tag="VFF05_10715"
     CDS             179..4771
                     /locus_tag="VFF05_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZW52326.1"
                     /translation="MPFPRGYGVAGRWLALFVALLAVSGHASERRYYFDDLGSERGLA
                     SHTITGLFQDNTGYMWIATQSGLDRYDGYEYRLFQHHAHDADSLPESFVTAVAQDAQG
                     RLWVGGLSQGLAALDPARGKVVARSRIGEQQARPRDAISALLFDPARGLWVGTAAGIE
                     LMNPVDRSRRELYRFPAGNKFPRVFQFHLAGNGALWAATSSGLLRFAPKSDVAQPVAS
                     GFLPLALCVLTGDDGVVYAGGTDGLFRVDATADSAQRLWPDTGPAPGMAQVRAMVRDR
                     RGRLWLSIFGTGLTIYDPANGKTESLRSNGRIPGSLPDDYVTTLLIDRSGLLWAGGSI
                     GGVATAQPGGAAFNYAMDPAPGRGQMGNSIRSLLEDGQGQLWLGTDGDGLKRYDPARD
                     TFEYFDGLFGNASATARDDLFPGVYVFALAHAGGDALWVGTNHGAFRFDPAKRTATPI
                     AVDAEHGNGLPGLAVRSLLAARDGSIWFGTFGQGLAHWIPAAAGQPEHWQQFRHRASE
                     ADSLASDIVVTLMQDGEGRVWIGTTDGLSVFDPGKGKLHTFRHDPSNPDSLSDNLIRS
                     LLQSGDGTLWIGTQSGLDRVDLPDAEPLHFTHYSKIGGSPSTTIFGLLEDAHHNIWLS
                     SNRGIATFNRALGAFFTYSLRDGLQGFEFNSGAAFARANGELVFGGLHGINRFDPDAI
                     GHDNYAPPAAITAVRIGDAKADMPASASGLHMRQGERVVRFEFAALDYAAPLRNRFVY
                     RLDGFDSDWIDAGSQHEAIYTNLPTGNYTFQVRAANADGVWSQSPATLSLWVTPPWWA
                     TAAMKTMYLALAAIALLGLLIIYRLRRQREERHARELREREDRLRLALWGSGDEFWDW
                     DVQRGHLYRIGADQLIGGQHEESLPQDTWKNQAVHPDDLAQVEKRVYDHLAGRTDHFE
                     SEHRIRNAQGAWTWVLSRGKVVDRDSNGRALRICGTAHNITEARRADRERRIASEVID
                     SMNEAVSVTDLDFRFVSVNRAFMRMTGYTEAECIGQPAQMLNSAKYSPDVFRTMREEF
                     ARNGHWRGELWQRRRDGEDFLAWLEVSQVRDDAGQITNYVGVMNDITDRKRAEQELRY
                     LANYDTLTGLPNRTLLGERLAHAVIRARRTSRKVAVLFLDLDRFKHVNDSMGHAAGDR
                     VLKAVGERLCEKIRESATVARLGGDEFTVVLEDVQHTDQVLRIASDLLDVFATPLTLD
                     SGQEVIISPSIGISLYPDHAQVPTDLLKFADTAMYQAKEQGRNMYQVYTPEMDAITRQ
                     RANMLGGLRLALERGELSLVFQPMLALDEGRITGVEALLRWHSSEFGEVPPSAFIPLA
                     EEAGLIERIGEFVLHRACVQLRQWLDQGLSGITMSVNLSALQLLRDELTQRLCEILAE
                     VQLEPQHLELELTESVLMANPERAIHTLDRLHAVGVAIAIDDFGTGYSSLSYLKRLPI
                     DRLKIDRSFVGDITTDPDDEAITKTIITMAHSLGLDVVAEGVETLEQLEYLHEQGCDE
                     IQGHWLSIPLDAASCFTFIREFERSRPPARSRLTVR"
     gene            4803..5027
                     /locus_tag="VFF05_10720"
     CDS             4803..5027
                     /locus_tag="VFF05_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HZW52327.1"
                     /translation="MSNANPALNELHEVSGRIDRLLELCRRLAEENRSLRQSQDQLAS
                     ERSNLLAKNEQARTRVEAMIARLRSLENGA"
     gene            5024..5326
                     /locus_tag="VFF05_10725"
     CDS             5024..5326
                     /locus_tag="VFF05_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZW52328.1"
                     /translation="MSGEGPIAVSILDREFLIGCTPEERPGLIAAAGYLDGKMREIRD
                     HARSAGVDRIAVMAALNIAHELIQLRQHRDTHDGTLAQHLQMLRAKLDGVLATSVK"
     gene            5332..5517
                     /gene="ssrS"
                     /locus_tag="VFF05_10730"
     ncRNA           5332..5517
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFF05_10730"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5545..6159
                     /locus_tag="VFF05_10735"
     CDS             5545..6159
                     /locus_tag="VFF05_10735"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZW52329.1"
                     /translation="MKRDSKRGDLRKRLRALRMQLPPRERAMAAAALAAMLEQLPEFL
                     VDAHIAGYWAVRDELPLHAAFASLRSRGQSYCLPLLTQSDTLQFAPWMPGAPLRNNRF
                     GIPEPDVPAVARLAPQILDVVLVPLVGFDRRGNRLGSGGGWYDRSFAFLRDVPRPARP
                     ILVGIGYHFQEVEKLDAQPWDIPMDFIATDRELIACTVTDAMTP"
     gene            6156..>6300
                     /locus_tag="VFF05_10740"
     CDS             6156..>6300
                     /locus_tag="VFF05_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZW52330.1"
                     /translation="MNFWLMKSEPDEFSIDALAKRKSEPWSGVRNYQARNYLREMRVG
                     DGVL"
BASE COUNT         1125 a         2083 c         1972 g         1120 t
ORIGIN      
        1 gacgatacgc atgagctgct tgcggcgacg ggcgaattcc ttgcgatcga tcatcgtggt
       61 ttcaatgcgc ggcgatgggt cattctatca ggccgcacgt acgggtcgcc gagcggttgc
      121 ctgcacggcg ccacatccct atcatcggga agtccaagtc catacaaagc gcgcatctat
      181 gccgtttccg cggggatacg gagtcgccgg aaggtggctg gcactgttcg tcgcactgct
      241 ggcagtgtcc ggacatgcca gcgagcggcg ctattacttc gacgacctcg gcagcgagcg
      301 cggcttggcc tcgcacacca ttaccggctt gttccaggac aataccggtt acatgtggat
      361 agccacgcag tcgggcctgg atcgctacga tggctacgaa taccggctgt tccagcacca
      421 tgcgcacgac gccgattcgc tgccggaaag ttttgttacc gccgtagccc aggacgcgca
      481 gggccgcctt tgggtcggcg gactgagcca gggtctggcg gccctggatc cggccagagg
      541 caaggtagtg gcacgctcgc gcatcggcga acagcaggcg cgtccacgcg acgcgatctc
      601 cgctctactg ttcgatccgg cgcgcggctt gtgggtcggc acggcagccg gcatcgaact
      661 gatgaatccg gtcgatcgca gccgccgcga actttaccgt tttccggccg gcaacaaatt
      721 cccgcgcgtc tttcagtttc atcttgccgg caacggcgcc ttgtgggcgg cgacgtcgtc
      781 cggcttgctg cgtttcgcac cgaaatccga cgtcgcccaa cccgtcgctt ccggcttctt
      841 gccgctggcg ctgtgcgttc tgaccgggga cgacggtgtg gtttatgccg gcggcaccga
      901 cggcttgttc cgcgtcgacg ccacggcgga ctccgcgcaa cgcctgtggc cggatacggg
      961 tccggcgccg ggcatggccc aggtgcgcgc catggttcgc gaccggcgcg gacgcttgtg
     1021 gctgtcgatt ttcggtacgg gcctgacgat ttacgaccct gccaacggca aaacggagtc
     1081 cctgcgcagc aacggccgca tcccgggttc gcttccagac gattacgtga cgaccttgct
     1141 gatagaccgt tccgggctgc tgtgggccgg cggcagcatc ggtggcgtgg caacggcaca
     1201 gcctggaggc gcggctttca actatgcgat ggacccggca ccgggccgcg gccagatggg
     1261 caacagcatc cgctcgttgc tcgaggacgg tcagggccag ctgtggctgg gaaccgacgg
     1321 cgacgggctg aaacgctacg atcccgcgcg cgataccttc gagtatttcg acggtctctt
     1381 cggcaatgca tccgcgacgg cgcgcgacga tctgtttccc ggcgtctatg tgttcgccct
     1441 ggcacacgcc ggcggcgacg cgctttgggt cggcaccaat cacggcgcat tccgtttcga
     1501 tcctgccaag cgcacggcca cgccgatcgc cgtcgatgcg gagcacggca atggcttgcc
     1561 gggtctggcg gtgcgctcgc tattggccgc acgcgacggc agcatatggt tcggtacttt
     1621 cggccagggc ctcgcgcact ggattccggc cgcggcgggc cagcccgagc attggcagca
     1681 gttccgccac cgcgccagtg aagccgacag cctggcgagc gacatcgtcg tcaccttgat
     1741 gcaggacggc gaaggccgcg tttggatcgg gaccacagac ggcctcagcg tcttcgatcc
     1801 gggaaaaggc aagctgcaca cgttccgcca cgatccgtcc aatccggatt cgctgtcaga
     1861 caacctcata cgcagcttgc tgcaaagcgg cgatggtacc ttgtggatcg gcacccagag
     1921 cggactcgac cgtgtcgatc tgcccgacgc ggaaccgttg cacttcaccc actattcgaa
     1981 aatcggcggg tcgccgagca cgaccatctt cggtcttctc gaagacgccc atcacaatat
     2041 ctggctgagc agcaaccgcg gcatcgccac gttcaaccgc gcactcggtg cgttctttac
     2101 ctactccctg cgcgatggat tgcagggctt tgagttcaac agcggcgccg ccttcgcacg
     2161 cgcgaacggc gagctggtat tcggcggatt gcacggcatc aaccggttcg atccggacgc
     2221 catcggccac gacaactacg cgccgccggc ggcgatcacc gccgtgcgca tcggcgacgc
     2281 caaggccgac atgccggcat cggcttccgg cctgcacatg cgccagggcg agcgcgtggt
     2341 tcgtttcgaa ttcgcggcac tcgattacgc tgctccattg cgcaaccgct tcgtctaccg
     2401 gctggacgga ttcgattcgg actggatcga cgccggcagc caacacgaag cgatctacac
     2461 gaacttgccg accggcaatt acacatttca ggtgcgtgcc gcgaacgccg acggggtctg
     2521 gagccagagt ccggccaccc tgagcctgtg ggtgacgccg ccatggtggg cgacggcggc
     2581 gatgaaaacc atgtacctgg cgcttgccgc cattgccttg ctcggattgt tgatcatcta
     2641 ccgtctgcgc cgccaacgcg aagagcggca tgcgcgcgaa ctgcgcgaac gcgaggaccg
     2701 cctgcgcctg gcattgtggg gttccggcga cgaattctgg gactgggacg tccagcgcgg
     2761 ccacctgtac cggatcggcg ccgaccagct gatcggcggc cagcacgagg aatccctgcc
     2821 gcaagacacc tggaagaacc aggccgtgca cccggacgac ctggcgcagg tggaaaagcg
     2881 cgtctacgac catctggccg gccgcaccga ccacttcgaa tccgaacacc gcatccgcaa
     2941 tgcgcagggc gcatggacct gggtgctgtc gcgcggcaag gtggtcgacc gcgacagcaa
     3001 cggccgcgcg ctgcgcatct gcggcaccgc gcacaatatc accgaggcac gcagggccga
     3061 ccgtgagcgg cgcatcgcca gcgaggtgat cgacagcatg aacgaggcgg tctcggtgac
     3121 cgacctcgat ttccgcttcg tttcggtgaa tcgcgcgttc atgcgcatga ccggttatac
     3181 cgaagccgag tgcatcggtc agcctgcgca gatgctcaac agcgccaagt actcgcccga
     3241 cgtcttccgc acgatgcgcg aggaattcgc gcgcaacggc cactggcgcg gcgagctgtg
     3301 gcagcgccgc cgcgatggcg aggatttcct ggcctggctc gaggtaagcc aggttcgcga
     3361 cgacgccggc cagatcacca attacgtcgg cgtgatgaac gacatcaccg accgcaagcg
     3421 cgccgagcag gaattgcgtt acctggccaa ctacgacacg ctgaccgggc tgccgaaccg
     3481 tacgctgctg ggcgaacgcc tcgcgcatgc cgtgatccgc gcgcggcgca catcgcgcaa
     3541 ggtcgccgtg ctgttcctgg acctggatcg cttcaagcac gtcaacgatt cgatgggcca
     3601 cgccgccggc gaccgcgtgc tcaaggcggt cggcgaacgc ctgtgcgaaa agatccgcga
     3661 atcggccacc gtcgcacgcc tgggtggcga cgaattcacc gtcgtgctcg aagacgtgca
     3721 acacaccgat caggtgctgc gcattgccag cgacttgctc gacgtcttcg ccacgccgct
     3781 cacgctggac agcggccagg aagtcatcat ctcgccctcg atcggtatca gcttgtatcc
     3841 ggatcacgcg caagtgccga cggatctgct caagttcgct gacaccgcga tgtaccaggc
     3901 caaggaacaa ggccgcaaca tgtatcaggt gtatacgccc gaaatggacg cgatcacgcg
     3961 ccagcgcgcg aacatgctcg gcggtctgcg cctggcgctg gagcgcgggg aattgagcct
     4021 ggttttccag ccgatgctcg ccctcgacga aggtcgcatc accggcgttg aagcgctctt
     4081 gcgctggcac agttccgaat tcggcgaggt tccgccgagt gcgttcatcc cgcttgccga
     4141 agaggccggg ttgatcgagc gcatcggcga attcgtcctg caccgtgcct gcgtgcaact
     4201 gcgccagtgg ctggatcaag gcctgtccgg catcaccatg tcggtcaacc tctcggcctt
     4261 gcaactgctg cgcgacgagc tgacccagcg cctgtgcgaa attctggccg aggtgcaact
     4321 cgagccccaa cacctggaac tggaattgac cgagagcgtg ctgatggcca atcccgaacg
     4381 cgcgatccac acgctcgacc gcctgcacgc ggttggcgtg gccatcgcga tcgacgattt
     4441 cggcaccggc tattcctcgc tcagctatct caagcgcctg cccatcgacc gtctcaagat
     4501 cgatcgcagt ttcgtcggcg atatcaccac cgacccggac gatgaagcaa tcaccaagac
     4561 catcatcacg atggcgcact cgctggggct ggacgtcgtc gccgaaggcg tggaaacgct
     4621 ggagcagctc gaatacctgc acgagcaagg ttgcgacgag atccagggcc attggctctc
     4681 gataccgctg gatgcggctt cgtgcttcac gttcatccgc gaattcgagc gcagtcggcc
     4741 gcctgcacgg tcgcgcctga ccgtgcgttg accctgcgcc cgcgcccgcc tatagtcccg
     4801 ccatgtccaa tgccaatccc gccctgaacg aactgcacga ggtcagcgga cgcatcgatc
     4861 gcctgctgga actgtgccgg cgcctggctg aggaaaaccg cagcctgcgc cagagccagg
     4921 atcagctcgc ctcggaacgt tcgaacctgc tcgcgaaaaa cgaacaggcg cgtacgcgcg
     4981 tcgaggcgat gatcgcgcgc ctgcgctcac tggagaacgg cgcgtgagcg gcgagggacc
     5041 gatcgcggtc agcatcctgg atcgtgaatt cctcatcggc tgcacaccgg aggagcgtcc
     5101 cggccttatt gccgcggcag gttatctcga tggcaagatg cgtgaaatcc gcgatcatgc
     5161 gcgttccgcc ggcgtcgacc gcattgccgt gatggcggcg ctgaacatcg cgcacgaact
     5221 gattcagctg cgccagcatc gcgacaccca cgacggcacc ttggcacaac atctgcaaat
     5281 gctgcgcgcc aagcttgatg gcgtactcgc aacatcggta aaatgatgcc gcgtcccctg
     5341 cggtgtgcga gagcgcatcc aaccatattg ccttgttcct atatacgacc ccggggtggc
     5401 agtgttcgag tccgtgtgca tgtccgccat gagcggaaag cctgacgacc ggcgagccat
     5461 cccacctgat cctagggatc aaggtcgttc ggtgtgcaac ggcatcgcgg agggcgccct
     5521 ctttttgtct tgcgatcgcc tcgcgtgaag cgcgattcca agcgcggcga tttgcgtaaa
     5581 cgcctgcgcg cattgcgcat gcagttgccg ccccgcgagc gcgcgatggc cgccgcagcg
     5641 ctggcggcaa tgctcgaaca gcttccggaa ttcctcgtcg atgcccacat cgctggctac
     5701 tgggcggtgc gcgacgaatt gccgctgcac gccgctttcg ccagcctgcg ctcgcgcgga
     5761 caatcctatt gcctgccgct gctcacccaa tccgacaccc tccaattcgc accttggatg
     5821 cccggcgcgc cacttcgcaa caaccgattc ggcattccgg agccggatgt ccctgccgtc
     5881 gcacgcttgg cgccgcagat actggacgtc gtgctggtgc cgctggtcgg attcgaccgt
     5941 cgtggcaacc ggctcggcag cggcggcggc tggtacgacc gcagtttcgc attcctgcgc
     6001 gacgtgccgc ggcctgcgcg cccgattctg gtcggcatcg gttaccattt tcaggaagtg
     6061 gagaaactcg atgcgcaacc ctgggatatt ccgatggact tcattgcgac cgaccgagaa
     6121 ttgattgcct gcaccgtgac ggacgcaatg acgccatgaa tttctggttg atgaaatccg
     6181 agccggacga gttttccatc gacgctctgg cgaaacgcaa atccgagccg tggagcggcg
     6241 tgcgcaatta ccaggcgcgc aactacctgc gcgagatgcg cgtcggcgac ggcgtgctct
//
LOCUS       DASRVO010000863         1407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_154497, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000863 DASRVO010000000
VERSION     DASRVO010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154497"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="sucB"
                     /locus_tag="VFF05_10745"
     CDS             complement(<1..1025)
                     /gene="sucB"
                     /locus_tag="VFF05_10745"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008572000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HZW52331.1"
                     /translation="MALEVKVPVLPESVSDATIASWHKKPGDAVKRDENLVDLETDKV
                     VLEVPAPVDGVLKEIKQQSGATVTSGQLLAVIEEGAVAAAPAAAPAAPKAAPAPAPAA
                     SPAAPAPSAAATNLSPAGQRVATENKIDPAQVAGTGVRGQVTKEDLVNFMRTGGSAPA
                     AAPAAKAPMTAVAPTPGSRPEERVPMTRMRAKIAERLMQSKNSIAMLTSFNEINLAKV
                     AAMRKELGEAFEKANGIKLGYMSFFVKAACEALKRHPIVNASVDGNDIIYHGYQDISV
                     AVSTDRGLVTPVLRDAQSMSFADVEKAITAYAKAAREGGLKLEDLQGGTFTITNGGTF
                     GSLFSTP"
     gene            complement(1050..>1407)
                     /gene="sucA"
                     /locus_tag="VFF05_10750"
     CDS             complement(1050..>1407)
                     /gene="sucA"
                     /locus_tag="VFF05_10750"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435566.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZW52332.1"
                     /translation="VYYDLVEDAARRGIGDVAILRVEQLYPFPRDEVTAQIARYHAAG
                     EVVWCQEEPMNQGGWYQIQHHLVACLTDEHSLHYAGRVRSPAPAAGHLPTHVAEQAAL
                     VEQALAGPAGADHSAD"
BASE COUNT          197 a          442 c          494 g          274 t
ORIGIN      
        1 atcggcgtgg agaacagcga accgaaggtg ccgccgttgg tgatcgtgaa cgtgccgcct
       61 tgcaggtctt cgagcttgag tccaccttcg cgtgcggcct tggcataagc ggtaatggct
      121 ttttccacgt ctgcgaaact catggactgc gcatcgcgca gcaccggcgt gaccaggccc
      181 cggtcggtgg acacggcgac ggaaatatcc tgatagccgt gatagatgat gtcgttgccg
      241 tcgaccgagg cgttgacgat gggatggcgc ttgagcgcct cgcaggcggc cttgacgaag
      301 aagctcatgt agccgagctt gatgccattc gccttctcga acgcttcgcc cagttccttg
      361 cgcatcgccg cgaccttggc caggttgatc tcgttgaacg aggtcagcat ggcgatggaa
      421 ttcttcgact gcatcaggcg ttcggcgatc ttggcgcgca tgcgtgtcat cggcacgcgc
      481 tcttccgggc gcgatcccgg tgtcggcgcg acggccgtca tgggcgcttt cgcggctggc
      541 gcggcggcag gagccgatcc gccggtgcgc atgaagttga cgaggtcttc cttggtcacc
      601 tgaccgcgca cgccggtgcc ggcgacttgc gcgggatcga ttttgttttc cgtcgccacg
      661 cgctggccgg cgggcgagag gttcgttgcg gccgcactcg gcgcgggtgc ggcgggagac
      721 gccgccggag ccggagccgg agcggctttc ggcgcggcgg gtgcggcggc tggagccgcg
      781 gcgacggcgc cttcctcgat cacggcgagc aactgcccgc tggtgacggt cgcgccggac
      841 tgctgcttga tttccttgag cacgccgtcg acgggtgccg gcacttccag cacgaccttg
      901 tcggtttcca ggtcgaccag gttttcgtcg cgcttcaccg cgtcgccggg tttcttgtgc
      961 cagctcgcga tggtcgcgtc gctgacggat tcgggcagta ccggaacttt gacttcgagt
     1021 gccattggaa atgtcctgtg actgtatttt taatcggcgg aatgatcggc gcctgccggc
     1081 ccggccaacg cctgctcgac cagcgcggcc tgctcggcga cgtgggtcgg caggtgtccc
     1141 gcggccggcg cgggcgagcg cacgcgaccg gcatagtgca gcgaatgctc gtcggtgagg
     1201 caggcgacca ggtgatgctg gatctggtac cacccgccct ggttcatcgg ctcttcctgg
     1261 caccagacga cttcgcccgc cgcgtggtag cgcgcgatct gcgccgtgac ctcgtcgcgc
     1321 gggaacggat agagctgctc gacgcgcagg atcgcgacgt cgccgatgcc gcgccgggcg
     1381 gcgtcctcga ccaggtcgta gtagacc
//
LOCUS       DASRVO010000864         1519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_25679, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000864 DASRVO010000000
VERSION     DASRVO010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25679"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VFF05_10755"
     CDS             <1..1162
                     /locus_tag="VFF05_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HZW52333.1"
                     /translation="GFSEPEAVLALIPGREHGEAILFCRERNPEREAWDGARAGQEGA
                     VRDFGMDDAFPIDDIDDILPGMLEGRSRVYYHFGRDADFDVQLIGWVNRVRARIKHGA
                     RAPHEFVALGHILHDLRLYKTRDELRVMRKAAQIATGAHVRAMRAVRPGMNEHEIEAE
                     LLHEFRRRGAVPSYEPIVGGGANGCVLHYRANNAPLRDGDLLLIDAGAEYECYASDIT
                     RTFPVNGKFSPEQRALYDVVLAAQRAAIDEVRPGKPFGAYHEAAVHTLTAGLIKLGLL
                     KGSVAKNIREQTYRKFYMHKTGHWLGLDVHDVGDYRVDGEFRELEPGMVVTVEPGLYI
                     APDAKGVAAKWRGIGIRIEDDVVVTKGDPEVITTAAPNDPDAIEALMAAARK"
     gene            1174..>1519
                     /locus_tag="VFF05_10760"
     CDS             1174..>1519
                     /locus_tag="VFF05_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52334.1"
                     /translation="MRRMCLAFLLFLPCVVAHAATVPVHFGLFGDVHVATPPGEPKRA
                     IVLVSDSSGWNTRAEALATAQADAGAFVLGIDLPAYLAQMESIDAKCSFPAGHFEEMI
                     HWIQRDRKLDKYI"
BASE COUNT          273 a          488 c          493 g          265 t
ORIGIN      
        1 cggcttttcc gaacccgagg cggtgctcgc gctgattccc ggccgtgagc atggcgaagc
       61 gattctgttc tgccgcgagc gcaatcccga gcgcgaagcc tgggatggcg cccgtgccgg
      121 gcaggaaggc gccgtgcgcg acttcggcat ggatgatgcg tttccgatcg acgacatcga
      181 cgacatcctg ccgggcatgc tcgaaggccg ctcgcgcgtc tactaccact tcggccgtga
      241 tgccgatttc gacgtgcagc tcatcggctg ggtcaaccgc gtgcgcgcac gcatcaagca
      301 cggcgcgcgt gcaccgcacg agttcgtcgc actcggccat atcctgcacg atctgcgcct
      361 gtacaagacg cgcgatgaat tgcgggtgat gcgcaaggcc gcgcagatcg ccaccggcgc
      421 gcatgtgcgc gcgatgcgcg cggtgcggcc gggcatgaac gaacacgaga tcgaggccga
      481 actgctgcac gaattccgcc ggcgtggcgc ggtgccgagt tacgagccca tcgtcggcgg
      541 cggcgccaac ggctgcgtgc tgcactaccg cgccaacaac gcgccgttgc gcgacggcga
      601 cctgctgctc atcgacgctg gcgccgagta cgagtgctac gcctcggaca tcacgcgcac
      661 gtttccagtg aacggaaaat tctcgccgga acaacgtgcg ctgtacgacg tcgtgcttgc
      721 ggcgcagcgc gcagccatcg atgaagtgcg tcccggcaag ccgttcggcg cttatcacga
      781 agccgcggta cacacgctca ctgccggcct gatcaagctc ggtttgctca aaggcagcgt
      841 cgcgaaaaat atccgcgaac agacctaccg caagttctac atgcacaaga ccgggcattg
      901 gctcggcctg gatgtccacg atgtcggcga ctaccgcgtg gacggtgaat tccgcgaact
      961 ggagccgggc atggtggtga cggtcgagcc cggcttgtac atcgcgcccg acgcgaaagg
     1021 cgtcgccgcg aagtggcgcg gcatcggtat ccgcatcgag gacgatgtcg tggtcaccaa
     1081 gggcgatccg gaagtcatca ccaccgccgc gccaaacgat ccggacgcga tcgaagcgct
     1141 gatggccgcc gcgcgcaaat agcggaatcg ttcatgcgca ggatgtgcct cgcgtttttg
     1201 ctgttcttgc cttgcgtcgt tgcgcacgcc gcgaccgtgc ccgtgcactt cggactgttc
     1261 ggcgacgtgc atgtagccac gcccccgggc gagcccaagc gcgcgatcgt gctcgtttcg
     1321 gattcgtcgg gatggaacac gcgcgccgag gcactggcga cggcgcaggc tgatgccggt
     1381 gcctttgtgc tcggcatcga tttacccgca tatctggcgc agatggaatc catcgacgcc
     1441 aagtgttcgt ttccggccgg gcatttcgag gaaatgatcc actggatcca gcgcgaccgg
     1501 aaactggaca agtatattt
//
LOCUS       DASRVO010000865         1018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_154520, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000865 DASRVO010000000
VERSION     DASRVO010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154520"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFF05_10765"
     CDS             <1..333
                     /locus_tag="VFF05_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HZW52335.1"
                     /translation="KLLGATASMVQVLVAQMDEALLPACLEIATQLRNAGIATEVVLD
                     GGKLGKQFKYADRAGIRFVVVLGTDEVAKGTVAVKDMRKQDQFEVSRAELVKILRVEL
                     EQTQVMPA"
     gene            330..>1018
                     /locus_tag="VFF05_10770"
     CDS             330..>1018
                     /locus_tag="VFF05_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HZW52336.1"
                     /translation="MSAETRHVVLLDGSHLTRQQVAQIARGDAIIGLAPDQLAKVQRT
                     ADFLAEQVKRGEPIYGVTTGFGSNADKLLGAHRARDELPGGNLETNSRTLMEELQHNL
                     VVTHAVCVGKPMSADVVRAMLAIRVNTLMRGHSGIRASTLQALAELLNRGIVPVIPEK
                     GSVGASGDLAPLSHLAIVLLGEGEAFYQGERLSGGEALKRAGLAPVKLSFKEGLALNN
                     GTTQMLAMGVLA"
BASE COUNT          194 a          308 c          352 g          164 t
ORIGIN      
        1 aagctgctcg gtgccaccgc gagcatggtg caggtgctgg tcgcgcaaat ggacgaggcg
       61 ttgttgccgg catgcctgga aatcgccacg cagctgcgca atgccggcat cgccaccgaa
      121 gtcgtgctcg acggcggcaa gctcggcaag caattcaagt acgccgatcg cgccggcatt
      181 cgttttgtcg tcgtgctggg taccgatgaa gtcgccaagg gcacggtcgc ggtgaaggat
      241 atgcgcaaac aggatcagtt cgaagtgtcc cgtgcggaat tggtgaagat cttgcgcgtg
      301 gaactggaac agacgcaggt gatgccggca tgagcgcgga aacgcgacat gtcgttctgc
      361 tcgacggcag ccacctgacg cgccagcagg tcgcgcagat cgcgcgcggc gacgcgatca
      421 tcggcctggc gccggaccaa ctcgccaagg tgcagcgaac ggcggacttc ctcgctgaac
      481 aggtcaagcg cggcgaaccg atctacggcg tcaccacggg attcggcagc aacgccgaca
      541 agctgctcgg cgcgcatcgc gcgcgcgacg aactgccggg cggcaacctg gagaccaaca
      601 gccgcacgtt gatggaggag ttgcagcaca acctcgtcgt cacgcatgcg gtctgcgtcg
      661 gcaagccgat gagcgccgac gtcgtacgcg cgatgctggc gattcgcgtc aatacgctga
      721 tgcgcgggca ttcgggaatc cgtgcgagca ccttgcaggc gctcgccgaa ttgctcaatc
      781 gcggcatcgt gccggtgatt ccggaaaaag gctcggtcgg cgcgagcgga gatctggccc
      841 cgctttcaca cctagcgatc gtgttgctcg gcgaaggcga ggcgttttac cagggcgaac
      901 gcctgtccgg cggcgaggcg ctcaagcgtg cggggctggc gccggtcaag ctctcgttca
      961 aggaaggcct cgcgctgaac aacggcacta cgcagatgct ggcgatgggc gtgctggc
//
LOCUS       DASRVO010000866         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_77529, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000866 DASRVO010000000
VERSION     DASRVO010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77529"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VFF05_10775"
     CDS             complement(<1..601)
                     /locus_tag="VFF05_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZW52337.1"
                     /translation="MFTTGTDSRKQPTPFGIKVLVVEDEPECLRRFCGAVAAEPALTL
                     VGSARTVAEACSLVDRVAPDVVLADLGLPDGRGIDVIRHSKAGDASRDVIVVTVFGDV
                     GHVIDSIRAGATGYLLKDALPRDIAGAILQVHDGGSPISPAIARRILQSLQVPDADHA
                     AADDAGSPGRLSARETQILRLVEKGLSFKEVGAALAISSH"
     gene            complement(847..>1181)
                     /locus_tag="VFF05_10780"
     CDS             complement(847..>1181)
                     /locus_tag="VFF05_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52338.1"
                     /translation="DNIERIAALARDATQLFIECTFLDEDADRAAGKKHLTARQAGTI
                     ARACGAKRLMPFHFSPRYSDREAQLRAEAEAAWSGSSDAAPASPTREPLAQPDPPAPR
                     AASRAAAR"
BASE COUNT          173 a          402 c          404 g          202 t
ORIGIN      
        1 tgtgcgacga gatcgcgagc gccgcgccga cctccttgaa actgagccct ttctcgacca
       61 ggcgcaggat ctgcgtttcg cgcgcggaca gtcggccggg actgccggca tcatcggccg
      121 ccgcatgatc cgcatccggc acctgaaggc tttgcaggat ccgccgcgca atcgcgggac
      181 tgatcggcga tccgccgtcg tgcacctgca ggatggcacc ggcgatgtcc cgcggcagtg
      241 catccttcag caggtacccc gtcgctccgg cgcggatgct gtcgatcacg tgtccaacgt
      301 cgccgaagac cgtcacgacg atcacgtcgc gtgacgcatc gcccgccttg ctgtgccgga
      361 tcacgtcgat gccgcggccg tccggcagac caagatccgc gagcacgacg tccggggcaa
      421 cccgatcgac gaggctgcac gcttcggcga cggtgcgtgc cgaaccgacc agcgtcaagg
      481 cgggctcggc cgccacggcg ccgcagaagc ggcgcaggca ctcaggttca tcttcgacca
      541 ccagcacctt gatgccgaag ggcgttggct gcttgcgcga gtcggtgccg gtcgtgaaca
      601 tggtgtcgcg ttcgtcgttt cggtttcgcg actttagacc gcatgcaggc tcagccacgc
      661 gaaatctttc tcctgttcgg ccggcgcgat agccgcaatc aaacgcagag cggccgaatc
      721 tgcgcgccgt cgcgtcctgc aacagttctt acgcgcgggg ctcttgcatc atcgcaatga
      781 tcggcccatc ggaggcggcg ttactggcgg tcgtgttcgc cgtgttctgc gcgcacgccg
      841 ccctgttcag cgggctgcgg cccgggacgc ggcgcgcgga gccggcggat ccggctgcgc
      901 gagcggctcg cgcgtcggtg aggcgggagc cgcatcggat gaccccgacc aggctgcctc
      961 ggcttcggcg cgcaattgcg cttcacggtc gctgtagcgc ggcgagaaat ggaatggcat
     1021 cagccgcttg gcgccgcagg cccgtgcgat cgtgccggcc tgccgcgcgg tgaggtgctt
     1081 cttgccggcg gcccgatccg cgtcctcgtc gaggaaggtg cattcgatga acaactgggt
     1141 cgcatcgcgc gcgagggccg cgatgcgctc gatgttgtcc g
//
LOCUS       DASRVO010000867         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_233501, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000867 DASRVO010000000
VERSION     DASRVO010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233501"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..2097)
                     /locus_tag="VFF05_10785"
     CDS             complement(190..2097)
                     /locus_tag="VFF05_10785"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HZW52339.1"
                     /translation="MSEKHADTVLASKPLARAVASAFGKPGLLAAATAGFVLSPTAFA
                     ATYTVGNLNDSGSSSLRDAIAQANANPGADTITFASNVTGTITLTSGSLYIYDGVSIQ
                     GPGASVLTIDGGASVTRPAAPSRITDSVIVINDGGRPQRPASALAATPVTISGLTITG
                     GYSNDGGGIDAYNADLQVNDCIVTGNYASHAGGGIYIGGYYSTLTVSRSTISNNFAYY
                     EGGGVATMEYGATITLQQSSLTGNQAKYGGGVYVHSDNGHALTITGSTISGNSVRSYS
                     YYYGGNNRSYGGLGAGVFVEHTPLTISNSTIANNNAARIGGGVFNFINDSGNYSATLS
                     NSTVSGNSANYAGGIVQSYNGTINLSNTVIAGNTAGEDADTGTPRGGDIAASFSLIGD
                     TGGSTISDNGGTLLNVAPQLGALADNGGPTMTMLPLAGSPLIDAGDPAFSGPATDQRG
                     AGFPRIINGRVDIGAVEFGTAAAATAVPAPGLGLGGQLGLGALLGLAGLAVVRRRRML
                     GAAAGVAFAAALAVSSPNVAHAAYKAQGGHESRHHEATTITSISVKGKIVTVVLGDGQ
                     TLKIRKGQLHTIDRRSTATRRQLRDPSSLSSGTPAAVHYEIGANGKVRDLRIRVVDTL
                     QQAQALVARKP"
     gene            2223..>3183
                     /locus_tag="VFF05_10790"
     CDS             2223..>3183
                     /locus_tag="VFF05_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HZW52340.1"
                     /translation="MQLPTEFIQLPLRFDAARLAAEISAMGEGAWRPHPQGFAGNDAM
                     PLVAAGGDPNDDATAGAMAPTPALRACPYLRQVLASFGSTIGRTRLMRIVGDGEVQAH
                     ADISYYWWERVRVHVPVITSPQARFQCGERAVNMAAGECWIFDTWREHRVSNPQPEAR
                     IHLVIDTPGSAHFWALAAAGRDPFAAQVTEFASTDIGFDAAVDPPLRYERVNRPRVMS
                     PWELATIAAWLEAEIEREAPGTEAARAQVRAALAALTQDWRSLWSAYGASGEGIAEYR
                     TLLDRLLTDTSAAAEGLHFRNKVAVYTALRSLIDEAALGKDVRA"
BASE COUNT          540 a          967 c         1093 g          583 t
ORIGIN      
        1 tccgtgcgca gacgcgccgc atcgatgctc aacggcaggc gcgcgcaggc gccgatcagt
       61 gcagttttgt cgaggggaga atcaggcaag tcgaacatgc gcaaagtcta gcgcagtcgg
      121 atgcgcgtcg cgctggcagg cggggcgaaa aacgatcccg gcgcgcgcgg gcgcgccggg
      181 tgcgagccat caaggcttcc gggcgaccag cgcctgggcc tgttgcagcg tgtcgacaac
      241 gcgaatgcgc agatcgcgca ccttgccgtt tgcgccgatt tcgtagtgca ctgcggcggg
      301 tgtgccactg ctcaagctcg aaggatcgcg cagctggcga cgcgtggcgg tgctgcgccg
      361 gtcgatggtg tggagctgcc ccttgcggat cttgagggtc tgaccatcac ccagcacgac
      421 ggtgacgatc ttgcccttca ccgaaatgct ggtgatcgtc gtcgcttcgt ggtgccttga
      481 ttcgtgtcca ccttgggcct tgtacgcggc gtgcgccaca ttcggcgaag aaacggcgag
      541 cgcagccgcg aacgcgactc ccgcagcagc gccgagcatg cgccggcgcc tcaccacggc
      601 cagtccggcc aacccgagca gggccccaag gccgagttgc ccgccgagac caagtccagg
      661 cgcgggtacg gcggtagcag ctgcggcggt tccgaattcg acggcgccga tgtcgacgcg
      721 gccgttgatg atgcgcggga atcccgcgcc gcgctgatcg gtcgccgggc cgctgaacgc
      781 gggatcgccg gcgtcgatca acgggctgcc ggccagcggc agcatggtca tggtcgggcc
      841 gccgttgtcg gccagcgcgc cgagttgtgg agcgacgttg agcagggtgc cgccattatc
      901 gctgatcgtg gagccgccgg tgtctccgat cagcgagaac gacgccgcga tgtcgcctcc
      961 gcggggcgtc ccggtgtctg cgtcctcgcc agccgtattg ccggcgatca cagtgttact
     1021 caggtttatc gtcccgttgt aactttggac gatgccgcca gcataattcg cgctgtttcc
     1081 cgagaccgtg ctgttgctca gcgttgcgga gtaattgccg ctgtcgttga taaagttgaa
     1141 aacaccgccg ccgatgcgcg cggcgttgtt gttggcgatc gtgctgttgc tgatggtaag
     1201 cggggtgtgc tcaacaaaaa cgccggcgcc caaaccgccg taagagcggt tattgccgcc
     1261 atagtagtag gaataggaac ggacactgtt gccggaaatg gtagagccgg tgatggtcaa
     1321 tgcgtgccca ttgtcactgt gcacgtacac gccgccaccg tacttcgcct gattgccggt
     1381 caacgaactt tgctggagcg tgatggtcgc gccatactcc atggtcgcca cgccgccacc
     1441 ttcgtagtag gcgaagttgt tgctgatcgt ggaacggctg acggtgaggg tggagtagta
     1501 tccgccaata tagattccgc cgccggcatg ggatgcgtag ttccctgtca ctatgcaatc
     1561 gttgacttgc agatcggcat tgtaggcatc tatgccaccg ccgtcgtttg agtatccgcc
     1621 ggtaatggtc aggccggaaa tcgtgactgg cgttgccgcc agcgcggagg cggggcgctg
     1681 tggacggccc ccgtcattga tgacaatcac gctgtccgta atacgggacg gcgcggcggg
     1741 acgggtcacg gaagcgccgc cgtcaatggt gagaacactg gcgccggggc cttggatact
     1801 cacaccgtcg tagatgtaca ggctgccgga cgtcagcgtg atggttccgg tgacattgct
     1861 ggcgaaagtg atcgtgtcag cgccggggtt cgcattggct tgggcaatcg cgtcgcgcag
     1921 gctgctcgag ccgctgtcgt tgagattacc gactgtgtag gtcgccgcaa acgcggtggg
     1981 cgagagcacg aatcccgcag tagctgcggc gagcaggcca ggttttccga acgcggaagc
     2041 gacggcgcgt gcaagcggct tggacgccaa aacggtatcg gcgtgtttct cggacatgcg
     2101 atttccccta aaaagtgtga accccgtcgc aatactagcc cgcaattccc gaccgggcaa
     2161 cacgttggga gatcgggccg cgatcggata agctgcggcg caattgcagc cttggggccg
     2221 acatgcagtt gccaaccgaa ttcatccagt tgccgttgcg tttcgacgcg gcgcggctgg
     2281 ccgcggaaat ttcagccatg ggcgaaggcg cctggcgacc tcatccgcag ggattcgccg
     2341 gcaacgacgc catgccgctg gttgccgcgg gcggcgatcc aaacgacgac gctacggccg
     2401 gagcgatggc gccgaccccg gcgttgcgcg cctgtccgta tctgcgccag gtgctcgcca
     2461 gcttcggctc gaccatcggc cgcacccggc tcatgcgcat cgtcggcgat ggcgaggtgc
     2521 aggcgcatgc cgacatcagc tattactggt gggagcgcgt gcgtgtacat gtgccggtga
     2581 tcacctcgcc gcaggcgcgt ttccagtgcg gcgaacgcgc ggtgaacatg gccgccggcg
     2641 agtgctggat tttcgacacc tggcgcgagc atcgggtgag caatccgcag cccgaggcgc
     2701 gcatccatct ggtgatcgac acgccgggat ccgcgcattt ctgggccttg gctgctgccg
     2761 gccgcgaccc cttcgccgcg caagttacgg agttcgcttc gaccgacatc ggtttcgatg
     2821 cggcggtcga tccgcccttg cgctatgaac gtgtaaaccg cccgcgcgtg atgtcgccat
     2881 gggaactggc cacgattgcg gcatggctgg aagcggagat cgaacgcgaa gcgccgggaa
     2941 ccgaggcggc gcgcgcgcag gtgcgggcgg cactggccgc cttgacgcag gattggcgct
     3001 cgctgtggag cgcctatggt gcgagcggcg aaggcattgc cgagtaccgc accttgctcg
     3061 atcgcctgct gactgacaca agtgccgctg ccgaaggatt gcatttccgc aacaaggtgg
     3121 ccgtctacac ggcgctgcgc tcattgatcg atgaggcagc gctgggcaag gacgtgcggg
     3181 cgc
//
LOCUS       DASRVO010000868         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_259247, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000868 DASRVO010000000
VERSION     DASRVO010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259247"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFF05_10795"
     CDS             <1..295
                     /locus_tag="VFF05_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HZW52341.1"
                     /translation="ITDGEKNARIPKDREPASLTEAECAELLAAAPLRPKRGRFGKAA
                     KGAPAKSATKASTTPAKKTATKKAAAKKTPVKKAAAKKPVARKAAPRAKAPAT"
     gene            300..857
                     /locus_tag="VFF05_10800"
     CDS             300..857
                     /locus_tag="VFF05_10800"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZW52342.1"
                     /translation="MDAALQAAATALRSGGVVAYPTEAVYGLGCDPHDRAAFERVFEL
                     KQRPPTQGVLLIAADFAQVEKYIDLTRVPADALARLRATWPGPYTWIVPRAAGVPDWI
                     AGSHAGIALRVTAHPLAAALCRAFGGALVSTSANRHGEPPARTAAAVHAAFGDELAYI
                     LDGAVGGLERPTPIRDAISGEPIRT"
     gene            872..>1542
                     /locus_tag="VFF05_10805"
     CDS             872..>1542
                     /locus_tag="VFF05_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZW52343.1"
                     /translation="MSAVVGIQAVSPAALPLLADADVSRIFSWRDGKPVTAGEFLDEV
                     CALAARLPSARHVVNLCDDRYRFTVAFCAATVTGQTNLLPGTRTPQAITETLAAYPDS
                     YVLAEHALDPAPARQFVLPEILRAGNASMPTLPPDRVVAIAFTSGSTGIPKANPKTWG
                     AVCASSSCNAAAVCKDGVPYIVATVPPQHMYGLELSVLLPLRSRAAIHSGQPFFPADI
                     ARALA"
BASE COUNT          263 a          544 c          508 g          227 t
ORIGIN      
        1 catcaccgac ggcgagaaga acgcgcgcat tcccaaggat cgcgaaccgg cctcgctgac
       61 cgaagccgag tgcgccgaac tgctggccgc ggcaccgctg cgacccaaac gcggacgctt
      121 cggcaaggcg gccaaaggcg cgccggccaa gtccgccacg aaggcaagta ccacaccggc
      181 gaaaaagacc gcaacgaaga aagcggccgc gaagaagact cccgtcaaaa aggccgcggc
      241 gaagaagcct gttgccagga aggccgcccc gcgcgcgaaa gcacccgcga cctgaaacga
      301 tggatgcggc actgcaagcg gcggcaacgg cgctgcgatc cggcggcgtc gtcgcttatc
      361 cgaccgaggc ggtctacggc ctcggctgcg atccgcacga tcgtgccgct ttcgagcgcg
      421 tattcgagct caagcagcgt ccgcccacgc agggcgtgct gctgatcgcc gcggattttg
      481 cgcaagtgga aaagtacatc gacctcacgc gcgtacccgc tgatgcgctc gcgcgcctgc
      541 gcgcgacctg gccgggcccg tatacctgga tcgtcccgcg cgccgcagga gtgccggact
      601 ggatcgcagg cagccatgcc ggcatcgccc tgcgcgtgac cgcgcacccg ctcgctgccg
      661 cactgtgccg cgccttcggc ggcgcgcttg tgtcgaccag cgccaaccgc cacggcgagc
      721 cgccggcacg cacggcggcc gcggtgcatg cggcattcgg cgatgaactt gcgtatattc
      781 tggacggcgc ggtgggcggc ctggagcggc cgactccgat ccgcgacgcg atcagcgggg
      841 aaccgatccg gacttgatcg ggggagggaa cgtgtcggca gtcgttggaa tccaagctgt
      901 ttcgccggct gcattgccat tgttggccga cgcggatgtg tcgcgcattt tttcatggcg
      961 agacggcaag cccgtaaccg cgggcgaatt cctggacgag gtctgcgcgc tggccgcgcg
     1021 gttgccgtcg gcgcgccatg tcgtcaacct gtgcgacgac cgctatcgct tcacggtcgc
     1081 gttctgtgct gccaccgtga ccggccagac gaacctgctg ccgggcacgc gcacgccaca
     1141 ggccatcacc gaaacgctgg ccgcctatcc ggacagttac gtgctcgccg aacacgcgct
     1201 cgatcccgcg ccggcgcggc aattcgtgct gccggaaatc ttgcgcgccg gcaacgcatc
     1261 gatgccaacg ctgccgccgg atcgcgtcgt tgccatcgcc ttcacctcgg gcagcaccgg
     1321 catcccgaag gcgaacccga aaacctgggg cgccgtgtgt gcgagcagtt cgtgcaacgc
     1381 cgcggccgtg tgcaaggacg gcgtgccgta tatcgtcgcg accgtgccgc cgcagcacat
     1441 gtacggactc gaactttccg tgctgctgcc actgcgcagc cgcgctgcga tccattcggg
     1501 gcaaccgttt ttccccgccg acatcgcgcg tgcgctcgcg ca
//
LOCUS       DASRVO010000869         5075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_388836, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000869 DASRVO010000000
VERSION     DASRVO010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388836"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VFF05_10810"
     CDS             complement(<1..1074)
                     /locus_tag="VFF05_10810"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HZW52344.1"
                     /translation="MTGFFVHRDSRTEALANALFDALDAERPAHVLAEQTIVVAHPGL
                     GRWLLGEFARRGSRGIAANFDLIQPWQWLERAAGNMLGGESDPAFKKENLRWHIHASL
                     PALDDARVQGLIDVDDGERRRFQLADRLAGVYAQYLIYRPDWIKDWEDGRSKDWQASL
                     WRRLRQRIPGPHRAQRHAQIVEALVARGDGASEPLHVFGVSHLPADVLAALRAVSTHR
                     AVHLYFPDPCREYWADLKSKRELLRKGEIDEEWHFEIGHPLLVSLGRMAQDFFIALEG
                     AGIELDAGEGDAQAQPSNLLTRLQDGIRTAESKPAEAIPATPADASLRVHACVTRLRE
                     LEVLKDALLRLLADDASLQPRDIV"
     gene            complement(1151..2485)
                     /locus_tag="VFF05_10815"
     CDS             complement(1151..2485)
                     /locus_tag="VFF05_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52345.1"
                     /translation="MKTLPTALLLAFGGSACAATLTVNSLADNTTGGDGLVTLREAIL
                     ASVNRTATDLSQTGTGSDTLDLTGLSGTIALNAVLPNIASQITIAGPGEALLTIQGSS
                     GNGGGDDGIFFVSDVLVASKVTLAGGTSQGGAGTSVQRDGAGGGAAGMGGAVFVNGGG
                     RFMADYVTFTGNRSIGGGGGSTNGPIGADYAGGGGGFGEDAPLMVVPPVYPAYGVHAT
                     RGGNGGPFGNTGGAAGTVGIYGFAGNGGDGGGGGGGGTGVATQGGNGAFAGGGGGAGF
                     ALCGAGPAGIGGYGGGGGGGTDSYTSFGTCTSIGGSGGTLGGNGGDGSFVSGQQNSGT
                     GGGGGGGAGMGGALFVRSGGMANLNECTFSSNQAQRGLGGSGGSNGMAGGNGQGKGGA
                     IFVMSGATVLVHNLVFTGNSAADAGSTPTDNGDIYGSVDADTIFRDGFDGGG"
     gene            complement(2593..3537)
                     /locus_tag="VFF05_10820"
     CDS             complement(2593..3537)
                     /locus_tag="VFF05_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZW52346.1"
                     /translation="MAAWPGALWYFPEPSIWAIALAMLGALWLLMPRGVPARVLGLFL
                     FLPLLWPAATPLADGEFMAYMLDVGQGEAFVVRTRNHAVIYDAGAKFPSGFDVGEATV
                     VPALHALGIEHVDRMVISHGDNDHSGGAAAVAAAWPVIAVESGEPDRLTFPATQCLAG
                     ESWTWNGVKFRVVHPREPLSSVDNDRCCVLAVRSGGSELVLPGDITRAVEPEVTAALA
                     PAPPHLILQVPHHGSKTSSSVDFIADLHPELGLITAGYMNRFHHPSKDVVARYAAAGV
                     DLLNSAQAGFVAIRFAPNAGPQVIEQGRRDRHPYWCEN"
     gene            complement(3553..4926)
                     /locus_tag="VFF05_10825"
     CDS             complement(3553..4926)
                     /locus_tag="VFF05_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HZW52347.1"
                     /translation="MRIRFPLADFFRPATVLALLAGALAVQTLSALPPRWIDAMLAAA
                     ALATIFLFRRARWIGFLLLAAAWTMWRADLSLAQRLPHALEGAAVVVSGQVHGLPRAQ
                     NDATRFEFDVDSATHEGKPIALSGRLRLAWYDTESSPAPVIAPCSDWRLRVRLKRPRG
                     GVNPGGFDFERYALEAGIVATGYVREDAANAQSGAALLCVDRLRERIGAAIDATLGDT
                     HAAHLLRALAFGDQHAMDEREWAVARATGIPHLIAISGLHIALFASFGIALVRLLWKL
                     SPRLTLRWPAPLIEAVASLVFAVAYALIAGFGLPTRRALVMIAALLVANLARRARAPV
                     HGLALAVIALLAWNPACVLSAGFWLSFVGVAWLMFCLGGAGERRRWWRELVTAQGVAS
                     LGLLPLGIWFFGQSSLVGPIANLVAVPWICFFVLPLTVVASLLVLVMPALGEPLLQIA
                     GWAMQSL"
     gene            complement(4973..>5075)
                     /locus_tag="VFF05_10830"
     CDS             complement(4973..>5075)
                     /locus_tag="VFF05_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014403069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein-releasing system ATP-binding protein
                     LolD"
                     /protein_id="HZW52348.1"
                     /translation="EIGTSLVLVTHDNTLARRMDSLLELHEGVLRTP"
BASE COUNT          993 a         1751 c         1550 g          781 t
ORIGIN      
        1 cacgatatcg cgaggttgca gcgacgcatc gtcggcgagc aagcgtagca gtgcatcctt
       61 gagcacttcg agctcgcgca ggcgagtgac gcaggcatgc acgcgcaggc ttgcatccgc
      121 gggcgttgcg ggaatcgctt ccgcaggttt cgactcggcg gtacgaatgc catcctgcaa
      181 gcgcgtgagc agattcgagg gctgcgcctg cgcatcgcct tcaccggcgt cgagttcgat
      241 gccggcgcct tccagcgcga tgaagaaatc ctgcgccatg cgcccgagcg aaaccagcaa
      301 cggatgccca atctcgaaat gccattcctc gtcgatttcg cccttgcgca aaagctcgcg
      361 cttcgatttg aggtcagccc agtattcgcg acaggggtcg gggaaataca ggtgcaccgc
      421 gcgatgcgtc gacacggcac gcagtgcggc gagtacatcg gccggcaaat gacttactcc
      481 gaatacgtgc agcggttcgg atgcgccgtc gccgcgtgcg accagcgctt cgacaatttg
      541 cgcatgccgc tgcgcccgat gtggcccggg gatacgctgg cgcagacgac gccagagcga
      601 agcttgccaa tccttcgagc gaccatcttc ccagtccttg atccaatccg gccggtagat
      661 caggtactgc gcgtagacgc cggcaaggcg atcggccaac tggaaacgtc gccgctcgcc
      721 gtcgtcgaca tcgatcaaac cctgcacgcg cgcgtcatcg agcgcgggca acgacgcgtg
      781 gatatgccag cgcagatttt cctttttgaa ggcgggatcg gattcgccgc caagcatgtt
      841 cccggcggcg cgttcgagcc attgccaggg ttgaatcaga tcgaaattcg ccgcgatgcc
      901 gcgcgatccg cgccgcgcga attccccgag caaccagcgc ccgagtcccg gatgcgcgac
      961 cacaatcgtc tgctcagcca gaacatgcgc gggtcgttcc gcatccagcg cgtcgaacag
     1021 cgcgttcgca agcgcttccg tgcgactgtc gcgatggacg aagaatccgg tcatccgggg
     1081 atgataccgg ctgacgtctc aattcccgcg atcagcccct ggcgcaagtc cgcagatggc
     1141 cacgaacgcc tcagccgccg ccgtcgaaac catcgcggaa aatcgtgtcg gcgtcgacgc
     1201 tgccgtagat gtccccattg tcggtcggtg tactgcccgc atccgctgcg gaattacccg
     1261 tgaagaccag gttgtggacg aggacggtcg cgccgctcat gacaaaaatc gcgcccccct
     1321 tgccctggcc attcccgccc gccatcccat tgctgccgcc gctgccgcca agtccacgtt
     1381 gtgcctgatt gctgctgaac gtgcactcat tcaagttggc catcccgccg gatcgcacga
     1441 acaaggcacc gcccatgccg gcaccgccgc caccgccgcc gccggtgccg ctgttctgtt
     1501 gtccgctcac gaacgatcca tcgccgccat tgccgcccaa tgtccctcct gaaccgccga
     1561 tcgatgtgca ggtgccaaac gaggtgtagc tgtccgttcc gccgccgccg ccgccaccgt
     1621 agccaccgat gccagccggg cccgcgccgc aaagtgcaaa accggcgcca ccccccccac
     1681 ccgcgaatgc cccattgcca ccctgtgtgg caactccggt tccgccaccg cctccaccgc
     1741 caccatcgcc gccattaccc gcgaagccgt aaatgccgac cgtgcccgcg gccccaccgg
     1801 tgttgccgaa cggcccgcca ttgccgccgc gcgtagcatg cacgccatac gccggataca
     1861 ctggcggaac caccatcagc ggcgcgtcct cgccaaaacc accgccacca ccggcataat
     1921 cagcaccgat tggaccattc gtgctgccgc ccccgccacc gatcgaacga ttcccggtga
     1981 aggtgacgta atctgccatg aatctgccgc cgccattgac gaagaccgca ccgcccatcc
     2041 ccgctgcgcc gccgccagca ccgtcgcgct gcacgctggt tcctgcgccg ccctgcgacg
     2101 tgccaccggc cagagtgact ttgctcgcca ccaggacatc ggagacgaaa aagatgccgt
     2161 cgtcgccgcc gccattgccg ctgctgccct gaatcgtcaa caaggcttcg cccggtccgg
     2221 caatcgtgat ctggctggca atgttcggca gcacggcatt cagcgcgatc gtgccgctca
     2281 agccagtcag atccagcgtg tcgctgcctg tgccggtctg actcagatca gtcgcggttc
     2341 ggttgaccga agccaggatc gcttcgcgca gagtgaccaa gccatcgccg ccggtcgtgt
     2401 tgtcggcgag gctgttgacg gtaagcgtgg cggcgcatgc gcttcccccg aacgccagca
     2461 acagggccgt gggcagcgtt ttcaacagta agccgcgtcc gcgacgatga ttccgatgat
     2521 ggacgaacat ttgcagtctc ctggtcgatt cgagcttctt gaccggtaaa cggcaaaagt
     2581 tctgacttcc tatcaattct cgcaccaata cgggtgccgg tcgcggcggc cctgctcgat
     2641 gacctgcggt ccggcattcg gcgcgaagcg gatcgcgaca aacccggcct gcgcggagtt
     2701 caacaggtca acgcccgcgg cggcgtagcg cgcaaccacg tccttgctgg gatgatggaa
     2761 gcgattcatg tagccggccg tgatcaggcc gagctccgga tgcaaatccg cgatgaaatc
     2821 aacgctcgac gaagtcttgc tgccgtgatg cggcacttgc aggatgagat gcggtggtgc
     2881 cggcgcgagc gcggcggtga cttcgggttc gacggccctg gtgatgtcgc ccggcaacac
     2941 cagttcgctg ccgccgctgc gcaccgcaag cacgcagcaa cggtcgttat cgacgctgga
     3001 cagcggttcg cgcggatgca cgacgcggaa tttcacgcca ttccaggtcc aggattcgcc
     3061 ggccagacac tgcgtcgcgg gaaacgtcag gcgatccggt tcaccggatt cgaccgcgat
     3121 gaccggccat gccgccgcga ctgccgctgc accgccggaa tgatcgttgt cgccgtggct
     3181 gatgaccatg cggtcgacat gctcgatgcc gagcgcatgc aaggccggca cgaccgtcgc
     3241 ttcgccgaca tcgaatcccg acgggaattt cgcacccgca tcgtagatca cggcgtgatt
     3301 gcgcgtacgc acaacgaagg cttcgccctg ccccacgtcc agcatgtagg ccatgaattc
     3361 gccgtctgcc agcggcgttg cggcaggcca cagcagcggc aggaacagga acagtccgag
     3421 cacgcgggcg ggaacgccac ggggcatcag caaccacaac gcacccaaca tggcgagcgc
     3481 aatcgcccat atcgacggtt ccgggaaata ccacagcgcc ccaggccacg cggccatttt
     3541 ttccagaagc cactacagcg attgcatggc ccagccggcg atttgcagca gcggttcgcc
     3601 gagtgcgggc atgaccagaa ccaggagcga agcaacgacg gtcagcggca gcacgaaaaa
     3661 acaaatccac ggcacggcga caagattcgc gatcgggccg accagcgagc tctggccgaa
     3721 gaaccagata ccgagcggca gcaggcccag gctcgccacg ccctgcgcgg tgacgagttc
     3781 acgccaccag cgacggcgct cgcccgcacc gccaaggcag aacatcagcc aagccacgcc
     3841 aacgaaggac aaccagaatc cggcactgag cacgcaggcg ggattccacg ccagcagcgc
     3901 gatgacggcg agtgccaatc catgcaccgg cgctcgcgca cgacgcgcaa gatttgccac
     3961 aagcagggcc gcgatcatca ccagcgcacg ccgcgtaggc aggccaaagc cggcaatcaa
     4021 tgcgtaggcg acggcgaaga cgagactcgc gacggcttcg atcagcggcg ccggccagcg
     4081 caaggtcagg cgcggcgaaa gtttccagag cagtcgcacg agggcgatgc cgaaactcgc
     4141 gaacagagcg atatgcaatc cggaaatcgc gatcagatgc gggataccgg tcgcgcgcgc
     4201 gaccgcccat tcgcgttcgt ccatcgcatg ctgatcaccg aacgcgagcg cgcgcagcag
     4261 atgcgcggca tgggtgtcgc cgagcgtcgc gtcgatggcg gcaccgatgc gttcgcgcag
     4321 acggtcgacg cacaacagcg cggcgccgga ttgcgcattc gccgcatctt ctcgcacata
     4381 accggtggcg acgatgccgg cttccagtgc gtagcgttcg aaatcgaatc cgccgggatt
     4441 cacgccgccg cgcgggcgct tcaggcgcac gcgcaggcgc cagtccgaac acggcgcgat
     4501 gaccggcgcg ggactggatt cggtgtcgta ccaggccagg cgcaggcgcc cggaaagcgc
     4561 aatcggcttg ccctcgtgcg tggccgaatc cacgtcgaac tcgaagcgtg tggcatcgtt
     4621 ctgcgcgcgc ggcaagccgt ggacctgccc gctgacgacc acggccgcac cttcgagtgc
     4681 gtgcgggaga cgctgcgcca gggaaagatc ggcgcgccac atcgtccatg ccgccgcgag
     4741 cagcaagaat ccgatccagc gcgcgcggcg aaacagaaaa atcgtggcga gcgccgccgc
     4801 tgcaagcatc gcgtcgatcc aacgtggcgg cagcgcggac aatgtctgca ccgcgagcgc
     4861 gccggccaac agagccagta ccgtggccgg acggaagaaa tccgccaatg gaaatcggat
     4921 gcgcaagacg actccccagt gtcccgtcat gctggccagg cgaaattcgc ctctatggtg
     4981 tgcgcaacac gccttcgtgc agctcgagca aactatccat ccgccgcgcc aacgtgttgt
     5041 cgtgcgtgac gagaacgaga ctggtgccga tctcg
//
LOCUS       DASRVO010000870         1542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_259453, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000870 DASRVO010000000
VERSION     DASRVO010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259453"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1542)
                     /locus_tag="VFF05_10835"
     CDS             complement(<1..>1542)
                     /locus_tag="VFF05_10835"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HZW52349.1"
                     /translation="VTDPTGDPTNGSPDIDLLAGFVAVDANTIYFRIDVADLQTQAPQ
                     ITSANATTFIVGQAGTFTVTSIGVPSAALALSGCTLPAGVTFVDNGNGTGTLAGTPGP
                     GTANTYACTLTASNGIPPNATQAFTLTVANAPSQTALVADINPSVFAQTVTFTATVTP
                     APPGSGTPTGTVNFLDGATNIGSGTLNGSGVATFATGTLPVATHPITAQYAGDVNFGP
                     STSSVVNQVVNQASQTITFTSTAPVGAAVGGTTYTVTATASSGLTVALSIDASAASVC
                     SLAGSTVSFTGVGNCVIDANQAGDANYTPALQVQQSFSVGKGSQTISFTSTAPAAAAV
                     GGSTYTVTATSSAGLTPVTLAIDAASSAVCSISGSTVSFTGVGTCTIDANQAGNANYN
                     AAPQAQQSFAVGKGSQTITFTSNAPPNAVVAGPTYTVTATASSGLSVALTIDATATSV
                     CSIAGSTVSFTASGTCVIDANQAGDANWNAAPQAQQSFAVSKNNQTITFSSTAPVGAV
                     VAGPSY"
BASE COUNT          244 a          519 c          499 g          280 t
ORIGIN      
        1 gtagctcggc ccggcaacca ccgcgcccac cggcgcggtc gagctgaacg tgatcgtctg
       61 gttgttcttg ctcaccgcga acgactgctg cgcctgcggc gcggcattcc agttcgcatc
      121 gcctgcctgg ttggcatcga tcacgcaggt accggaggcc gtgaacgata cggtcgagcc
      181 cgcgatcgag cacaccgaag ttgccgttgc gtcgatcgta agagcgaccg acaagccgga
      241 cgatgccgtc gccgtcaccg tgtaggtcgg gccagccacg acggcgttcg gcggcgcgtt
      301 cgaggtgaac gtgatcgtct ggctgccctt gccgacagcg aagctctgct gcgcttgcgg
      361 cgccgcgttg tagttcgcgt tgccggcctg atttgcgtcg atcgtgcagg ttcccacgcc
      421 cgtgaacgac accgtcgagc cggaaatcga gcacactgcg ctcgatgccg catcgatggc
      481 gagcgtcacc ggcgtcaggc ccgcgctcga tgtcgccgtc accgtataag tcgaaccacc
      541 cacggcggct gccgccggcg cggtcgacgt gaacgaaatc gtctggctgc ccttgccgac
      601 ggagaaactc tgctgcactt gcagtgctgg cgtgtaattg gcatcgcctg cctgattggc
      661 gtcgatcacg cagttgccca ccccagtgaa cgacacggtc gagcctgcga gcgagcacac
      721 gctcgccgcg ctggcgtcga tcgagagcgc cactgtcaga cccgaactcg ccgttgccgt
      781 caccgtgtag gtcgtaccgc cgaccgcggc gcccaccggc gcggtcgagg tgaatgtgat
      841 cgtctggctg gcctggttga cgacctggtt gacgacactg ctcgtgctcg gcccgaaatt
      901 cacgtcgccc gcgtactgcg cggtgatcgg atgcgtggct accggcaagg tgccggttgc
      961 gaacgtcgcc acgccactgc cgttgagcgt gcccgatccg atgtttgtcg cgccatccag
     1021 aaagttgacg gtacccgtcg gcgtgcccga tccgggcggc gccggcgtta ccgtcgccgt
     1081 gaacgtgacc gtctgcgcaa acaccgacgg gttgatgtcc gcaaccagtg ccgtctggct
     1141 cggcgcattc gcgaccgtca gcgtgaacgc ctgcgtcgcg ttcggcggaa tgccgttact
     1201 cgccgtcagc gtgcatgcgt acgtgttcgc cgtacccgga ccgggcgtac ccgcaagcgt
     1261 gccggtacca ttgccgttgt ccacgaaggt cacgcccgcc ggcaacgtgc acccgctcaa
     1321 cgcgagcgcc gcggacggta cgccgatgga cgtcaccgtg aacgtacccg cctggccgac
     1381 gatgaacgtc gtcgcattcg cactggtgat ctgcggcgcc tgcgtttgca gatccgccac
     1441 gtcgatgcga aagtaaatcg tattcgcgtc gactgccacg aatcccgcga gcaggtcgat
     1501 gtccggcgaa ccgttggtcg gatctcctgt gggatcggtc ac
//
LOCUS       DASRVO010000871         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_154709, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000871 DASRVO010000000
VERSION     DASRVO010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154709"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="VFF05_10840"
     CDS             complement(<1..133)
                     /locus_tag="VFF05_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZW52350.1"
                     /translation="MTQPEETRRRRTFAIISHPDAGKTTLTEKLLLFAGAIRIAGSVK
                     "
     gene            complement(259..1641)
                     /locus_tag="VFF05_10845"
     CDS             complement(259..1641)
                     /locus_tag="VFF05_10845"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HZW52351.1"
                     /translation="MAVSTFDLFKIGIGPSSSHTVGPMRAAARFVTRWLEEGGVLDRV
                     TRVRAELFGSLAHTGRGHGTDKAVLCGFEGEWPDKIDPDSIPARLERIRSRKKINLLG
                     KREIAFDEKTDLIFNKRTKLPYHSNGMRFTAFDAAGEMLATRDYYSVGGGFVVNPDDA
                     AENRIVADTTTLPHPFSSGDELLKRCADSGKSIAQVMFENEKAWRSESEIRAGLLAIW
                     KAMSDCVARGMRESGTLPGGLHVQRRAPAMCNELKERPEAALKDPLTILDWVNLYALA
                     VNEENAAGGRVVTAPTNGAAGIIPAVLHYYHRFVPGANEQGVLDFLLTAAAIGLLYKE
                     NASISGAEVGCQGEVGVACSMAAGGLTAALGGTIYQVENAAEIGMEHNLGLTCDPIGG
                     LVQIPCIERNAMGSVKAINAQRMALRGDGKHRVSLDKVIRTMRDTGRDMQDKYKETSR
                     GGLAVNVIEC"
     gene            1685..1912
                     /locus_tag="VFF05_10850"
     CDS             1685..1912
                     /locus_tag="VFF05_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HZW52352.1"
                     /translation="MRLTLYQRDDCHLCDLALDVLAQARVPEFSSVFIDDDAELEARY
                     GERVPVLRNEDSGCELNWPFDAAAVMKFNGC"
BASE COUNT          327 a          653 c          680 g          403 t
ORIGIN      
        1 ccttgacgct gcccgcgatg cgaatcgcgc cggcgaacag cagcagcttc tcggtcaggg
       61 tggtcttgcc cgcgtccgga tgcgagatga tcgcgaacgt gcgccggcgc cgggtttctt
      121 caggttgggt catgggaaat cgggagtcgt tatgcactca ccccttgccc tctcccgcaa
      181 gcgggcaagg gggtgatcgt tgcgctgcgc gcaacatcgg gattggaatc aagcgggcgc
      241 gggattatac gcgacgagtc agcactcgat cacattcacc gccaggccac cgcgcgaggt
      301 ttccttgtat ttgtcctgca tgtcgcggcc ggtgtcgcgc atggtcctga tgaccttgtc
      361 cagcgacacg cggtgcttgc cgtcgccgcg cagggccatg cgctgcgcgt tgatcgcctt
      421 gaccgagccc atggcgttgc gctcgatgca gggaatctgc acgaggccgc cgatcggatc
      481 gcaggtgagt cccaggttgt gttccatgcc gatctcggcg gcgttctcga cctggtagat
      541 cgtgccgccg agcgcggcgg tgagtcctcc cgcggccatc gagcaggcga caccgacctc
      601 gccctggcag ccgacttcgg cgccggagat cgaggcgttt tccttgtaca ggagtccgat
      661 cgccgcggcg gtgagcagga aatccagcac gccttgctcg ttcgcaccgg gcacgaagcg
      721 gtggtagtaa tgcagcaccg ccgggatgat gcccgccgcg ccgttcgtcg gcgcggtgac
      781 gacgcgaccg cccgcggcgt tttcctcgtt gaccgcgagg gcgtacaggt tcacccagtc
      841 gaggatcgtg agtggatcct tgagcgcggc ctcggggcgt tccttgagtt cattgcacat
      901 cgccggcgcg cgccgttgca cgtgcaggcc gccgggcagg gtgccggatt cgcgcatgcc
      961 gcgcgcgacg caatcggaca tggccttcca gatggccagg agcccggcgc ggatttccga
     1021 ttcgctgcgc caagctttct cgttctcgaa catgacctgc gcgatcgact tgcccgagtc
     1081 ggcgcaccgc ttcagtaatt cgtcgccgct gctgaacgga tgcggcagcg tcgtggtgtc
     1141 ggcgacgatg cggttctcgg cggcatcgtc gggattgacc acgaaaccgc caccgacgga
     1201 gtaatagtcg cgcgtggcaa gcatttcgcc ggccgcgtcg aacgcggtga agcgcatgcc
     1261 gttgctgtgg tagggcagct tggtgcgctt gttgaagatg aggtcggttt tctcgtcgaa
     1321 cgcgatctcg cgcttgccga gcaggttgat cttcttcctc gaacggatgc gttcgagccg
     1381 tgccggaatc gaatcgggat cgatcttgtc gggccattcg ccctcgaaac cgcacagcac
     1441 ggccttgtcc gtgccgtggc cgcgtccggt gtgcgcgagc gatccgaaca gttccgcgcg
     1501 cacgcgcgtc acgcgatcca gcacgccgcc ttcctccagc cagcgcgtga cgaaacgcgc
     1561 cgccgcacgc atcgggccga ccgtatgcga cgacgacggg ccgatgccga ttttgaacag
     1621 atcgaaggtg ctgaccgcca cgtcgatatt ccttgccagc tatcgcgggt attctacgct
     1681 tgccatgcgc ttgacactct accaacgcga cgactgccac ctgtgcgatt tggcgctgga
     1741 tgtcctggcg caggcgcgcg tgccggagtt ctccagcgta ttcatcgacg acgatgccga
     1801 actggaagca cgctatggcg agcgcgtgcc ggtgttgcgc aatgaagatt ccggatgcga
     1861 gctgaactgg cctttcgatg cggcggccgt gatgaagttc aacggatgct gaaaacaaag
     1921 gccgcgctag gcggcctttg tgcgcgagtt cgctcgacgc attagctcag agcagcgact
     1981 tggcgttatc gagcacggcg ccgcatgcct ttttcgtcag cttggatttc atgtcgccga
     2041 cgccggccat gccgcccgcg cca
//
LOCUS       DASRVO010000872         1478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_25920, whole genome
            shotgun sequence.
ACCESSION   DASRVO010000872 DASRVO010000000
VERSION     DASRVO010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25920"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1478)
                     /gene="priA"
                     /locus_tag="VFF05_10855"
     CDS             complement(<1..>1478)
                     /gene="priA"
                     /locus_tag="VFF05_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZW52353.1"
                     /translation="LLRGGPAAYSRLDAQFPEWRSAAANLRRRGLIETVRIDATSPPP
                     VCIAPPPLTDEQGAAIAAIAAESGKFAPFALDGITGSGKTEVYLALAVQALARGEQVL
                     VLVPEIGLTPQLLRRFRERLGAPVHALHSDLADGERTRAWLAAARGESCVVLGTRSAV
                     FVPLPRAGLIVVDEEHDTSYKQQDGLRYHARDLALVRGKALNVPVVLGSATPSLETLA
                     NVEVGRYRHLHLSSRPGAARMPAVRTIDLRGKRLADGLAPELIAGIRACLARGEQALL
                     FRNRRGYAPVLLCRACGWHAACARCDKPLTWHRSEARLRCHHCGAQQKVPAACPNCDG
                     TDLTPHGLGTERMEQALAGLFPGTPIVRIDRETARGKDAVENLLNSIAPDQPGILVGT
                     QMLAKGHDLPNLTFVGLIGVDSGLFSADFRASERLCQLIVQVSGRAGRALKPGQVWLQ
                     THHPDHPLLRDLLKFGYAQVARELLVERREAGLPPYAHLALLR"
BASE COUNT          253 a          484 c          517 g          224 t
ORIGIN      
        1 cgcaacaacg ccaaatgagc gtagggcggc agcccggcct cgcggcgctc gaccagcagt
       61 tcgcgtgcga cttgcgcata gccgaatttc agcaaatcgc gcagcaacgg gtgatccgga
      121 tgatgcgtct gtagccagac ctggccgggt ttcaacgcgc gtccggcgcg cccggaaacc
      181 tgcacgatga gctggcacag gcgctcgctg gcgcgaaaat ccgcgctgaa caggccgcta
      241 tccacgccga tcaggccgac aaaagtcagg ttcggaagat catggccctt ggccagcatc
      301 tgggtgccga cgagaattcc gggttgatcc ggtgcgatcg agttgagcag attttcgacc
      361 gcgtccttgc cacgcgcggt ttcgcgatcg atgcgcacga tgggcgtgcc cggaaaaagt
      421 cccgccagtg cttgttccat gcgctccgta ccgaggccat gtggcgtcag atcggttccg
      481 tcgcaattcg gacaggctgc cggcaccttc tgctgcgccc cgcagtgatg gcagcgcaac
      541 cgcgcctcgc tgcgatgcca ggtcagcggc ttgtcgcagc gcgcacacgc ggcgtgccag
      601 ccgcaggcgc ggcacagcag cacgggggca tagccgcggc ggttgcggaa cagcagggcc
      661 tgctcaccgc gtgcgagaca cgcgcggatg ccggcgatca gctccggcgc caaaccatcg
      721 gcgaggcgct tgccgcgcag atcgatggtg cgcacggcgg gcatgcgcgc tgcgccggga
      781 cgcgaggaca gatgcaggtg gcggtagcgc ccgacttcga cattcgccag cgtttccagc
      841 gagggcgtgg cagaaccgag cacgacggga acattgagcg ccttgccgcg cacgagggcg
      901 agatcgcgcg cgtggtagcg cagcccgtcc tgctgcttgt acgacgtgtc gtgttcctcg
      961 tcgacgacga tgaggcctgc gcgcggcagc ggcacgaaca ccgccgagcg cgtgccgagg
     1021 acgacgcagg attcgccgcg cgcggcggcg agccaggcgc gcgtgcgctc gccatcggcc
     1081 agatccgaat gcagtgcgtg cacgggtgcg ccaaggcgtt cgcggaaacg ccgcagcaac
     1141 tgtggcgtca ggccgatttc cggaaccagt acgagcacct gttcgccacg cgccagcgct
     1201 tgcacggcga gtgcaagata gacctcggtc ttgccgctgc cggtgatgcc gtcaagtgca
     1261 aacggcgcga attttccgga ttcggccgcg atcgccgcaa tcgcggcgcc ttgttcgtcc
     1321 gtcagtggcg gtggcgcgat gcacacgggc ggcggcgaag tcgcatcgat acggacggtt
     1381 tcgatgagcc cgcgccggcg cagattggcc gcggcgctgc gccattcggg aaattgcgcg
     1441 tcgagcctcg aatacgctgc cggtccgccg cgcagcag
//
LOCUS       DASRVO010000873         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_362577, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000873 DASRVO010000000
VERSION     DASRVO010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362577"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFF05_10860"
     CDS             complement(<1..587)
                     /locus_tag="VFF05_10860"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015448896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZW52354.1"
                     /translation="MSKKPSVAVLGAGSWGTALAAVLARNGVPTTLWGRDADAMARMA
                     DTRRNTRYLPDLELPAALTLSADLAGTVRGANVVLIVTPSHAFAGLLAAIAPHFPAGA
                     GLAWATKGFDPPSGRFLHEIAAERLPAAPAAIVTGPSFAREVAQGLPTAVTVHSADAD
                     FAQRIADLLHSPRFRAYTGNDMIGAELGGAMKNVLA"
     gene            complement(622..1095)
                     /gene="secB"
                     /locus_tag="VFF05_10865"
     CDS             complement(622..1095)
                     /gene="secB"
                     /locus_tag="VFF05_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008211953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HZW52355.1"
                     /translation="MADETTQAAPAAGAQLSLQKVYIKDASFEVPSAPQIFQEQGQSQ
                     VQLNLQQQIGTLADNVYEVVLTVTVTCKLADKTAYLAEVQQAGIFGVAGFDEANRDMV
                     LATFCPNVLFPYARTAVANMIQDGGFPPFLLQPINFEALYDEQMRRRAEGAQPNA"
     gene            complement(1167..>1541)
                     /locus_tag="VFF05_10870"
     CDS             complement(1167..>1541)
                     /locus_tag="VFF05_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008216573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZW52356.1"
                     /translation="GLIAAILGNEIARRFRGFRELTPGALTLLINRENPLLVDLSSAA
                     DFEKGHIAGARHVAMSQFDPENKELAKVKELPVALVCKTGATSAQAAARLVKAGFAKV
                     FVLGGGMGAWREAALPTAKGSK"
BASE COUNT          261 a          490 c          526 g          264 t
ORIGIN      
        1 gcgagcacgt tcttcatcgc cccgccgagt tccgcgccga tcatgtcgtt gccggtgtag
       61 gcacggaaac gcgggctgtg cagcaagtcc gcaatgcgct gtgcgaagtc ggcgtcggcc
      121 gaatgcacgg tcaccgcggt cggcaggcct tgcgcgactt cgcgcgcgaa cgagggtccg
      181 gtgacgatcg cggccggtgc ggcgggcagg cgttcggcgg cgatctcgtg caggaaacgt
      241 ccggacggcg gatcgaagcc cttggtcgcc caggcgagac ccgcgccggc cgggaagtgc
      301 ggcgcgatcg cggccagcag accggcgaag gcgtggctgg gagtgacaat cagcacgaca
      361 ttcgcgccgc gaacggtgcc ggccaaatcg gcgctcaggg tcagcgccgc gggcaattcc
      421 aggtcgggca gatagcgcgt gttgcggcgt gtgtcggcca tgcgtgccat ggcatccgcg
      481 tcgcggcccc acaacgtggt gggcacgcca ttgcgcgcga gcacggcggc cagcgccgtg
      541 ccccatgagc cggcgccgag tacggccacg gaaggctttt tactcattct tgaatttcgc
      601 gatcgcggaa gatcgcaaaa atcacgcgtt cggctgcgcg ccttcggcac gacggcgcat
      661 ctgttcgtcg tagagcgcct cgaaattgat cggctgcagc aggaagggcg ggaaaccgcc
      721 gtcctggatc atgttcgcca cggccgtgcg cgcgtagggg aacagcacgt tcggacagaa
      781 cgtggccaac accatgtcgc ggttggcctc gtcgaaaccg gccacgccga aaatgccggc
      841 ttgctgcact tcggccaggt acgcggtctt gtcggcgagc ttgcaggtga cggtgacggt
      901 aagcaccacc tcgtagacgt tgtccgccaa cgtgccgatc tgctgctgca ggttgagctg
      961 cacctgtgac tggccctgtt cctggaagat ctgcggggcg ctgggcacct cgaacgaggc
     1021 gtccttgatg tagaccttct gcagcgacaa ctgtgcgccg gcggcagggg cggcttgggt
     1081 ggtttcgtcg gccatgggaa actccgaatg ctttgcgggg aaaaagggcg cggattatcc
     1141 catgcggcgc caatcaacgc gagtttttac ttgctaccct tggccgtcgg cagcgccgct
     1201 tcgcgccacg cgcccatgcc gccgccgagc acgaagacct tggcgaaccc ggccttgacc
     1261 aggcgtgcgg cggcctgggc cgacgtcgca cccgtcttgc acaccagcgc cacgggcaat
     1321 tccttgacct tggccagttc cttgttttcc ggatcgaact ggctcatcgc gacgtgacgc
     1381 gcgccggcaa tgtggccctt ctcgaagtcg gccgcactgg acaaatccac cagcagcgga
     1441 ttttcacggt tgatgagcag ggtcagcgca ccgggcgtga gttccctgaa accgcggaaa
     1501 cggcgcgcga tttcgttgcc gagaatcgcc gcgatcaaac c
//
LOCUS       DASRVO010000874         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_259630, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000874 DASRVO010000000
VERSION     DASRVO010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259630"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VFF05_10875"
     CDS             <1..324
                     /locus_tag="VFF05_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52357.1"
                     /translation="GRGLLHRLLRRAGGLVARRAVPVAAIVSSAVANASVTYAIGKRA
                     QAAARLPQAPITGMPDALRAFTGVDERRIAAWSLGAVKSVLAGIGKAAKRAASTVRPA
                     KSRRA"
     gene            complement(321..>1846)
                     /locus_tag="VFF05_10880"
     CDS             complement(321..>1846)
                     /locus_tag="VFF05_10880"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HZW52358.1"
                     /translation="REQELLARQSSPDNDQALTALRASASQMDTALRETRERMQLVQK
                     QMAQNDQQRQEAQAKFDQARAQLALEHAQNENSKQLLKLMEAQLSEKRQELASQRTQM
                     ASLQSQIDGAKYTSLPPGFATASLGGDLRVEILQPALAVTRGLNKPAASVPPAQNGID
                     VVGRVQAPAGLTSVTLNGQTVAVGAGGVFKTHVALTGGADTAVRVAASDKTGGEAVLD
                     FMLVPGPGGKAVRESGPSGQSLPSGVRLGKYYALIIGNDSYAAFPALGSAAEDAKAVA
                     GVLQSRYGYDVKLLANANRFEILSALNDMREALTDKDNLLVYYAGHGEIDSSRQGYWL
                     PVDAQLNQPNSWISNRAISDILTTMQARHVLVIADSCYSGTMTRSALATFGGGMANNV
                     WGDWVKTMTAGRSRTALTSGGVQPVADAASKGGHSVFATALLTVLRDNNQLLTGQEMF
                     REIAAGMALRSANAGLQQAPEYAPIQFAGHEAGEFFLMPENAGRKTAALGKPHGDGV"
BASE COUNT          267 a          619 c          609 g          351 t
ORIGIN      
        1 ggccgcggtt tgctgcatcg actgttgcgc cgcgccggcg gtctggtggc acggcgcgcg
       61 gtgccggtag ccgccatcgt ttcgtcggca gttgcaaatg cgtccgtgac ttatgcgatc
      121 ggcaagcgtg cacaggcggc ggcgcgcctg ccgcaggcgc ccatcaccgg catgcccgat
      181 gcgctgcgcg cgttcaccgg tgttgacgag cgccgcatcg cggcatggtc gctgggcgcc
      241 gtcaaaagcg tgctggccgg catcggcaag gcggccaagc gtgccgcctc gaccgtacgg
      301 ccggcgaaat cgcgccgcgc ctagacgccg tcgccgtgcg gcttgccgag cgcggcggtc
      361 ttgcgcccgg cgttttcggg catcaggaag aactcgcctg cctcgtgtcc ggcaaactgg
      421 atcggcgcgt attccggtgc ttgttgcaag cccgcgttcg cgctgcgcag ggccatgccg
      481 gcggcgattt cgcggaacat ttcctgtccg gtgagcagtt ggttattgtc gcgcagcacg
      541 gtcagcagcg ccgtcgcgaa caccgaatgc ccgcccttgc tggcggcgtc cgccaccggc
      601 tgtacgccgc cggaggtcag cgccgtgcgc gaacgtcccg cggtcatggt cttgacccag
      661 tcgccccaca cgttgttggc catgccgccg ccgaacgtcg cgagcgccga gcgcgtcatc
      721 gtgcccgaat agcacgagtc ggcgatcacc agcacgtgtc gtgcctgcat ggtcgtgagg
      781 atgtcgctga tcgcgcgatt ggaaatccag ctgttgggct ggttgagctg cgcatccacc
      841 ggcagccagt agccctggcg cgaggaatcg atttcgccgt gccccgcgta atacacgagc
      901 aggttgtcct tgtcggtcag cgcttcgcgc atgtcgttga gcgcggacag gatttcaaaa
      961 cggttcgcgt tcgccagcaa tttgacgtcg tagccatagc gcgattgcag cacgccggcc
     1021 acggccttgg catcttccgc cgcgctgccc agggccggga acgcggcgta actgtcgttg
     1081 ccgatgatca gtgcgtaata cttgcccaag cgtactccgg agggcagcga ctgcccgctc
     1141 ggtcccgatt cgcgcacagc cttgccgccc gggccaggca cgagcatgaa gtcgagcacc
     1201 gcttcaccgc cggtcttgtc ggatgcggcg acgcgaaccg ccgtgtcggc gccgccggtc
     1261 aacgccacgt gagtcttgaa gacgccgcct gcaccgaccg ccacggtctg gccattgagc
     1321 gtgaccgaag tcaggcctgc cggcgcctgc acgcgtccca ccacgtcgat accattctgc
     1381 gcaggaggca ccgatgccgc cggcttgttc aagccgcgcg tgacggcaag cgcgggttgc
     1441 aggatttcca cgcgcaaatc cccgcccaaa ctggccgtgg cgaatcccgg cggtagcgac
     1501 gtgtacttgg caccgtcgat ctggctttgc aaactcgcca tctgcgtgcg ttggctggca
     1561 agttcctgac gtttttcgga caactgcgct tccatcagct tgagcagttg tttcgaattc
     1621 tcgttttgcg cgtgctcaag ggcgagctgc gcgcgcgcct gatcgaactt cgcctgcgcc
     1681 tcctggcgct gctggtcgtt ctgcgccatc tgtttctgca caagttgcat gcgctcgcgg
     1741 gtttcgcgca gcgcggtatc catttgcgaa gccgatgcgc gcagtgccgt gagggcctgg
     1801 tcgttgtccg gcgaggactg ccgcgccagc aattcctgct cgcgcg
//
LOCUS       DASRVO010000875         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_259718, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000875 DASRVO010000000
VERSION     DASRVO010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259718"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..211)
                     /locus_tag="VFF05_10885"
     tRNA            complement(137..211)
                     /locus_tag="VFF05_10885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(177..179),aa:Lys,seq:ttt)
     gene            complement(237..917)
                     /gene="queC"
                     /locus_tag="VFF05_10890"
     CDS             complement(237..917)
                     /gene="queC"
                     /locus_tag="VFF05_10890"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZW52359.1"
                     /translation="MKRAVVLVSGGMDSAVTLALAREQGFACHALSVDYGQRHVSELT
                     AAQNVARALGAVEHKTVHVDLRSIGGSALTADIDVPVDSPLGGGGADIPVTYVPARNT
                     IMLSVALGWAEVLGAQDIFCGVNAVDYSGYPDCRPEFIAAFEKLANLATKAAVEGAAL
                     RVHAPLICLSKAQIASEGLRLHVDFAATVSCYQADADGRACGHCDACRLRAQGFSDAG
                     VADPTRYR"
     gene            complement(920..1579)
                     /gene="queE"
                     /locus_tag="VFF05_10895"
     CDS             complement(920..1579)
                     /gene="queE"
                     /locus_tag="VFF05_10895"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HZW52360.1"
                     /translation="MSEIDSAGRLRVTEIFHSLQGEARDAGRPTVFVRLTGCPLRCVW
                     CDTEYAFQGGQWRDIADIVDEVDKYRTRHVCVTGGEPLAQKRCLDLLARLCDAGFEVS
                     LETSGALDVGGVDARVSRVMDLKAPGSGESARNLWSNLAALTAHDQIKFVLADRTDYD
                     WAVAAVNEHALAGRCEILFSPAWGKLAPRDLAQWILDDRLPVRMQLQLHKILWGEERG
                     K"
     gene            complement(1587..2483)
                     /gene="ybgF"
                     /locus_tag="VFF05_10900"
     CDS             complement(1587..2483)
                     /gene="ybgF"
                     /locus_tag="VFF05_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HZW52361.1"
                     /translation="MKRVLRSLQAIPVAALVAATVFAMPAQAQRLSLAERVARLEQEQ
                     GQGGAQANIQMVNQINALQSQLQNLQGQIEELRHQVDELKSHGKDQYIDLDSRISRLE
                     GRNPTAAPATSGGANPNGAAMQDVQLGNPQATQAPAAVPVAGTGTPGATQAPAQADAS
                     AAAAPPATAQDPKSAYDAAFGALKDGRYAESARLFQQFITQFPNDELTPNAYYWLGES
                     YYVTQNYPVSLDTFRKLLTQYPNSQKAPDALLKVGYCQYEMKQWDQAQVTLNQVLQQY
                     PNSTVANLAQGRLRALRIEAQR"
     gene            complement(2487..>2805)
                     /gene="pal"
                     /locus_tag="VFF05_10905"
     CDS             complement(2487..>2805)
                     /gene="pal"
                     /locus_tag="VFF05_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HZW52362.1"
                     /translation="FDFDKSEIKPEFQAQIACHAAYLRQFPGARVTLEGNADERGTRE
                     YNLGLGERRGNAVDSAIAAAGASASQLNVVSYGEERPVCKEHNEGCWQKNRRVEIVYT
                     AKQ"
BASE COUNT          470 a          894 c          913 g          528 t
ORIGIN      
        1 atcgattcgc gcgtactcgg tatttccgga attcgaaaaa gcgcgcggtc acatcgcgcc
       61 gggatgcgcg gccgatcttg ccgcgctgga tgcggatttc gcagtgcgcg gaatgcgaga
      121 atcgcaagat tggaactggt gggccgtggt gggatcgaac caccgaccaa tagattaaaa
      181 gtctactgct ctaccgctga gctaacggcc cgggaatgca cgcgggggcg cgcagtttaa
      241 cggtagcgtg tcggatcggc aacgccggca tcgctgaatc cctgcgcgcg caggcggcag
      301 gcgtcgcaat gcccacaggc gcgcccatcg gcatcggcct ggtagcacga caccgtcgcc
      361 gcgaagtcga cgtgcaggcg caggccttcg ctcgcgatct gcgccttgct caggcagatc
      421 agcggtgcgt gcacgcgcaa cgcggcgcct tcgacagcgg ccttggtggc gaggttcgcg
      481 agtttctcga acgcggcgat gaattccggc cggcaatccg gatatccgga ataatcgacc
      541 gcattgacgc cacagaagat gtcctgcgcg ccgaggacct cggcccaacc cagcgccacc
      601 gacagcatga tcgtgtttcg cgccggcaca taggtgacgg gaatgtcggc gccgcccccg
      661 ccgaggggag aatcgacggg aacgtcgatg tcggcggtca gtgccgagcc gccgatgctg
      721 cgcaggtcca catgcacggt cttgtgttcg acggcgccga gagcgcgcgc cacgttttgc
      781 gcggcagtca attcggaaac gtgacgctgg ccgtagtcga cgctcaacgc gtggcaggca
      841 aatccctgct cgcgcgcgag cgccagcgtc accgccgaat ccatgccacc ggaaaccagc
      901 accactgccc ttttcatcgt cactttccgc gttcctcgcc ccacaggatc ttgtgcaact
      961 gcaactgcat gcgcaccggc aggcgatcgt cgagtatcca ttgcgccaga tcgcgcggcg
     1021 ccagttttcc ccacgccggc gagaacagga tttcgcagcg cccggcgagc gcgtgttcgt
     1081 tcactgccgc gacagcccag tcgtaatcgg tgcggtcggc gagcacgaac ttgatctggt
     1141 cgtgcgccgt cagcgcggcg agattgctcc acagattgcg cgcggactcg ccggagccgg
     1201 gcgccttcag gtccatgacg cggctcacgc gcgcatcgac gccgcctacg tccagcgcgc
     1261 cggaggtttc cagcgacact tcgaatccgg cgtcgcacag tcgcgcgagc agatcgaggc
     1321 agcgcttttg cgccaggggt tcaccgccgg tgacgcagac gtggcgcgtc cggtacttgt
     1381 cgacttcgtc gacgatatcg gcgatatcac gccactgtcc accctggaac gcgtattcgg
     1441 tatcgcacca cacgcagcgc agcggacagc cggtcaggcg cacgaatacc gtcggccggc
     1501 ccgcgtcgcg cgcttcgcct tgcagggaat ggaaaatttc ggtgacgcgc aggcgtccgg
     1561 ccgaatcgat ctcggacatc gaacgcttaa cgctgtgctt cgattcgaag cgcccgcaac
     1621 cggccttgcg caaggttcgc cacggtcgaa ttgggatatt gctgcagaac ttggttcaag
     1681 gtgacctgcg cctgatccca ctgcttcatt tcgtactggc aatagccgac cttgagcagc
     1741 gcgtcgggcg ccttctggct gtttggatac tgcgtcagca gcttgcggaa ggtgtcgagg
     1801 gaaaccggat aattctgggt gacgtagtag ctttcgccga gccagtagta ggcgttcggg
     1861 gtcaactcgt cgttcggaaa ttgggtgatg aattgctgga acagacgcgc cgattcggcg
     1921 tagcgtccgt ccttgagtgc accgaatgca gcatcgtagg ccgatttcgg atcttgcgcc
     1981 gtagccggtg gcgcggcggc agccgaagca tccgcctgtg ctggcgcctg ggtcgcgccg
     2041 ggagtgccgg tgcctgccac gggcactgcg gccggtgcct gggtcgcctg cggattgccg
     2101 agctgcacgt cctgcatggc ggcgccattc ggattcgcgc cacccgaggt ggcgggagca
     2161 gcggtcggat tgcgcccttc cagacggctg atgcgcgagt ccagatcgat atactgatcc
     2221 ttgccatggc tcttgagttc atcgacctga tgacgcagtt cttcgatctg gccttgcaga
     2281 ttctgcaact gcgactgcag cgcattgatc tggttgacca tctggatatt ggcctgcgca
     2341 ccgccctggc cctgctcctg ttcgagcctg gccacgcgct ccgcaaggct tagccgctgc
     2401 gcctgcgcgg gcatggcgaa gacggtggca gccaccaggg ctgccaccgg gatcgcttgc
     2461 agtgagcgca agacgcgctt cattgcttac tgcttcgccg tgtagacgat ttcgacgcgg
     2521 cggttcttct gccagcagcc ttcgttgtgc tccttgcaca ccgggcgctc ttcgccgtag
     2581 ctgacgacgt tgagctggct cgcagaggcg ccagcggcgg caatcgcgct gtccacggcg
     2641 ttgccgcggc gctcgccgag gccgaggttg tactcacgcg tgccgcgttc gtcggcgttg
     2701 ccttccaggg tcacgcgcgc gcccgggaac tggcgcagat acgcggcgtg gcaggcgatc
     2761 tgggcctgga attccggctt gatctcgctc ttgtcgaaat cgaaa
//
LOCUS       DASRVO010000876         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_154960, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000876 DASRVO010000000
VERSION     DASRVO010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154960"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFF05_10910"
     CDS             complement(<1..404)
                     /locus_tag="VFF05_10910"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HZW52363.1"
                     /translation="MAHNAELFLGLISGTSADGIDVALARFEPAPRLHAALTHPYPDD
                     LRQRILALAQGDGRIALDELGTLDVEIARSFAVAANALLQREGIAASSITALGSHGQT
                     VRHRPRQTPPYTLQLGDPNVIAEITGIATVAD"
     gene            complement(410..1735)
                     /locus_tag="VFF05_10915"
     CDS             complement(410..1735)
                     /locus_tag="VFF05_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZW52364.1"
                     /translation="MHAWLRKATAAPLSWRHRHWLLAGILFAAVAIIGLVTPRGANAT
                     RDPVASDFITLNLPVPPLPSGAEDVAAVAGSDADWTTVSVKSGQTVGAIFHAQGVSGN
                     DLQTLLQDASNTNALVRIHPGEEFSFAHAADGHLTALRFDRDEKTQVTVTFDANGMHQ
                     ATAARDVERRTEVAHGVITDSLFDAANRAGMDDAMVIKLADAFGYDIDFAQDLRPGDS
                     FTVIYDSVWREGERLRDGDILAAYFVNRGKRFSAIRYTDNHGGTLFYSADGRPLRKSF
                     LRTPVEFTRISSFFSLARKHPILGYTRAHQGVDYAAPTGTPIHAAGDGTIVFRGWKSG
                     YGNFVIIQHNKHISTAYGHMSRFANERVGQRVHQGQTIGFVGMTGLATGPHLHYEFRV
                     DGTHRNPLTVTLPKPEPLPATELARFKQQSAPMLAKLDRLDSLQLASAK"
     gene            1995..>2515
                     /gene="tyrS"
                     /locus_tag="VFF05_10920"
     CDS             1995..>2515
                     /gene="tyrS"
                     /locus_tag="VFF05_10920"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZW52365.1"
                     /translation="MNDTVTALEQIARGAEEIIKREDLEARLKLGRPLRVKAGFDPTA
                     PDLHLGHTVLLNKMRQFQDLGHQVIFLIGDFTGMIGDPSGKNATRKPLTREDVLANAE
                     TYAAQVYKVLDREKTELRFNSEWFGKMDAAGMIRLAAKHTVARMLERDDFEKRYKSGQ
                     PIAIHEFLYPLVQG"
BASE COUNT          483 a          738 c          851 g          443 t
ORIGIN      
        1 aaatccgcca cggtggcgat gccggttatt tcagcgatca cgttgggatc gccgagttgc
       61 aaggtgtagg gtggcgtttg ccgtgggcga tggcgcacgg tttgaccgtg tgagccgagc
      121 gcagtgatcg agctggcggc gatgccttcg cgttgcaaca acgcattggc cgccaccgca
      181 aagctgcgcg cgatctcgac atccagcgtg ccgagttcat ccagcgcgat gcggccgtct
      241 ccctgggcga gggcgaggat gcgctggcgc aaatcatcgg ggtacggatg cgtcagtgcg
      301 gcatgcaggc gcggcgccgg ctcgaaacgc gccaacgcga catcgatgcc gtcggcgctg
      361 gtgccggaga tgagcccgag gaaaagctcg gcgttgtgcg ccacgaagac tacttcgcgg
      421 aagccagttg caggctgtcg agtcggtcga gcttggccag catgggtgcg gactgctgct
      481 tgaaacgcgc cagttccgtg gccggcagcg gctcgggctt tggcaacgtc acggtcagcg
      541 gattgcgatg cgtgccgtcc acgcggaatt cgtaatgcag gtgtggtccc gtcgccaatc
      601 ccgtcatgcc gacgaaaccg atcgtttgac cctgatgcac gcgttggccg acccgctcgt
      661 tggcgaagcg cgacatatgg ccgtaggcgg tgctgatgtg cttgttgtgc tggatgatga
      721 cgaagttgcc gtagcccgat ttccagccgc ggaacacgat cgtgccgtcg ccggcggcgt
      781 ggatcggtgt accggtcggc gcggcgtagt cgacgccctg gtgcgcgcgc gtgtaaccga
      841 ggatcggatg cttgcgcgca agactgaaga acgaggaaat acgcgtgaac tcgaccggtg
      901 tacgcaggaa ggatttgcgc agtggtcggc cgtcagcgct gtagaacagg gtgccaccgt
      961 ggttgtcggt gtaacgaatg gcggaaaagc gcttgccgcg gtttacgaag taggcagcca
     1021 gaatatcgcc atcgcgcagt ctttcgcctt cgcgccagac gctgtcgtag ataacggtga
     1081 aactgtcgcc ggggcgcaga tcctgggcga agtcgatgtc gtagccgaac gcgtcggcga
     1141 gcttgatcac catcgcatcg tccatgccgg cgcgattggc ggcatcgaac agcgaatcgg
     1201 tgatcacgcc gtgcgcgact tcggtgcggc gctctacgtc gcgagccgcg gtggcctggt
     1261 gcatgccgtt cgcatcgaac gtgacggtga cctgggtctt ctcgtcgcgg tcgaagcgca
     1321 gcgcggtgag atgcccgtca gcggcgtggg cgaaggaaaa ttcctcgccg ggatggatgc
     1381 gtaccaaggc gttggtgttg ctcgcatcct gaagcagggt ctgcaggtcg ttgccggaca
     1441 caccttgcgc gtggaagatc gcgccgacgg tctggccgga tttcacggag acggtagtcc
     1501 aatccgcatc gcttccggca acagcggcaa catcctcggc accggatggc agcggcggca
     1561 ccggcaagtt caacgtgatg aagtcgctgg ccactggatc gcgcgtggcg ttcgccccgc
     1621 gcggcgtcac caagccgata atggcgacgg cggcaaacag gattccagcc agcaaccagt
     1681 gccgatgccg ccagctcagc ggcgccgccg tggccttgcg caaccaggca tgcacggcct
     1741 gcgatttata gaaatggaaa tgccggcgcg gcaaatgtcg cccgcgcgct gcgcgatgag
     1801 cggtgagtcg gataaaatgc gcaacgcgat ttgtttctgg cacgagttcg gtccggcaaa
     1861 cgcgccgtac cttagcccct gtatcggacc ccgtcaacgc caagcgcgac aagggtttgc
     1921 gcagggtttt gcggttaacg aataattaac gacgcgtacc gtttttggtg cgcgcggcgt
     1981 cgaaaaggca cgacatgaac gacacggtga cagcgctcga gcagatcgcc cgcggcgccg
     2041 aggaaatcat caagcgcgaa gacctggaag cgcggctcaa gcttggccga ccgctgcgcg
     2101 tgaaggcggg attcgatcca accgcgccgg acctgcacct cggccacacc gtgctgctca
     2161 acaagatgcg ccagttccag gacctcggcc accaggtgat tttcctgatt ggcgatttca
     2221 ccggcatgat cggcgatccc agcggcaaga acgccacgcg caaaccgctc acgcgcgaag
     2281 acgtgctggc caatgccgaa acgtatgcgg cgcaagtata caaagtgctg gatcgcgaga
     2341 agaccgagct gcgcttcaac tcggaatggt tcggcaagat ggacgccgcc ggcatgatcc
     2401 ggctggccgc gaagcatacg gtcgcgcgca tgctcgaacg cgacgatttc gagaagcggt
     2461 ataaaagtgg acagccgatc gcgatccacg aattcctcta tccgctggtg caggg
//
LOCUS       DASRVO010000877         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_155172, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000877 DASRVO010000000
VERSION     DASRVO010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155172"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..>1078)
                     /gene="nadB"
                     /locus_tag="VFF05_10925"
     CDS             complement(55..>1078)
                     /gene="nadB"
                     /locus_tag="VFF05_10925"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF006567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZW52366.1"
                     /translation="VYLYTTNPDLSTGDGIAMAWRAGCRVSNMEFIQFHPTCLYHPNV
                     KSFLISEAVRGEGGRLLLPDGSRFMQFHDERGELAPRDVVARAIDFEMKKRGIDCVYL
                     DISHKPADFVREHFPTIYGRCLELGIDITRQPIPVVPAAHYTCGGVVTDMNARTDLCN
                     LHAVGEVTFTGLHGANRLASNSLLECLVFGHAAARDILATPPAPSLSLPPWDASRVTD
                     PDEEVVISHNWDELRRFMWDYVGIVRTNKRLARASHRIRLLRAEIDEFYRNFRVSNDL
                     IELRNLVQSADLIIRSAQLRQESRGLHYSRDYPETLPIAGNTVLNPRPGTLCPSVCGV
                     PSEATP"
BASE COUNT          170 a          325 c          401 g          182 t
ORIGIN      
        1 tgcgcaccct ggtgttgctg ccagacagtg cggatgccga cgacctgcgc cgactcaggg
       61 tgtcgcttcg ctgggcacgc cgcacacgct cggacacagc gtcccgggac gcgggttgag
      121 cacggtgttg ccggcgatcg gcagcgtctc ggggtagtcg cggctgtagt gcaggccgcg
      181 gctttcctgg cgcaactggg ccgagcggat gatgaggtcg gcgctctgca ccaggttgcg
      241 cagctcgatc aggtcgttgc tgacgcggaa gttgcggtag aactcgtcga tctcggcgcg
      301 cagcaggcga atgcggtgcg acgcgcgcgc gagccgcttg ttggtgcgca cgatgccgac
      361 atagtcccac atgaagcgcc gcagttcgtc ccagttgtgc gagatcacca cctcctcatc
      421 ggggtcggtg acgcgcgacg catcccacgg cggcaggctg agcgaggggg cgggcggtgt
      481 ggccagaatg tcgcgggcgg cggcgtggcc gaacaccagg cattccagca acgagttcga
      541 ggccagccgg ttggcgccgt gcagcccggt gaaggtgact tcgccgacgg catgcaggtt
      601 gcacagatcg gtgcgcgcgt tcatgtcggt gacgacgccg ccgcaggtgt agtgcgcggc
      661 cggcaccacg gggatcggct ggcgcgtgat gtcgatgccg agttccaggc agcggccata
      721 gatggtcggg aaatgctcgc gcacgaaatc ggccggcttg tggctgatgt cgagatagac
      781 gcagtcgatg ccgcgcttct tcatctcgaa gtcgatcgcg cgcgccacca cgtcgcgcgg
      841 cgccagttca ccgcgttcgt cgtggaactg catgaaacgg ctgccgtccg gcagcagcag
      901 gcggccgcct tcgccgcgca ccgcctcgct gatcaggaac gacttgacgt tggggtggta
      961 caggcaggtc gggtggaact ggatgaattc catgttcgac acgcggcagc cggcgcgcca
     1021 cgccatggcg atgccgtcgc cggtggagag gtcgggattg gtggtgtaga ggtagacc
//
LOCUS       DASRVO010000878         1147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_103928, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000878 DASRVO010000000
VERSION     DASRVO010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103928"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1147
                     /locus_tag="VFF05_10930"
     CDS             <1..>1147
                     /locus_tag="VFF05_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010487240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZW52367.1"
                     /translation="SGYRGSPLGGIDQGMHGAKKHLEAHHVKFQPGVNEDLAATAIWG
                     TQQANLFPGAKYDGVFAMWYGKGPGVDRCGDVFKHGNAAGTSKNGGVLALAADDHACR
                     SSTLPHGSELEFVSAMMPVLNPAGVQDILDLGLLGWAMSRFTGRWVGFKTIAETVESS
                     ASVIVDPHQFDIVLPQDFELPPNGLNIRWPDPPLDQEMRLHQYAVKAACAFARANGID
                     RVVLDSPKARLGIVTTGKSYLDVLQALEYLGISAADARTIGIRVYKVGMTWPLEPVGI
                     REFARGLEDIIVVEEKRSFIESQMKEYMFNWDARPHIVGKYDEDGNWILPSTGELTPA
                     MIALVIAKRLSRFFTSEAMAARVAWIDAKEHELTLPRANFPRVPHYCS"
BASE COUNT          216 a          374 c          363 g          194 t
ORIGIN      
        1 ctccggttat cgcggctcgc ccctgggagg aatcgatcag ggaatgcacg gcgcgaagaa
       61 gcacctcgaa gcccaccacg tcaaattcca acccggcgtg aacgaagact tggcggcaac
      121 ggcgatctgg ggcacgcaac aggcgaacct gttcccgggc gcgaagtacg acggcgtgtt
      181 cgcgatgtgg tacggcaagg gcccgggcgt ggaccgctgc ggcgacgtgt tcaagcacgg
      241 caacgcggcc ggtacctcga agaacggcgg cgtgcttgca ctcgccgcgg acgatcacgc
      301 ctgccgttcc tccacgctgc cgcacggatc ggaactcgaa ttcgtctcgg caatgatgcc
      361 ggtgctcaat ccggccggcg tgcaggacat cctcgacttg ggcttgctcg gctgggccat
      421 gtcgcgcttc accgggcgct gggtcggttt caagacgatc gccgaaaccg tcgaatcgtc
      481 ggcgtcggtc attgtcgatc cgcaccaatt cgatattgtg ctgccgcaag atttcgaact
      541 gccgccgaac ggactcaaca tccgctggcc cgatccgccg ctggatcagg aaatgcgcct
      601 gcaccagtac gcggtcaagg cggcttgcgc gttcgcgcgc gcgaacggca tcgaccgtgt
      661 cgtgctcgat tcgccgaagg cgcgcctggg catcgtcacc accggcaaga gttatctcga
      721 cgtgctgcag gcgctggaat acctcggcat cagcgcggcc gatgcgcgca cgatcggcat
      781 ccgcgtctac aaggtcggca tgacctggcc gctggaaccc gtcggcatcc gcgagttcgc
      841 gcgcggactc gaagacatca tcgtggtcga ggaaaaacgt tccttcatcg aatcgcagat
      901 gaaggagtac atgttcaact gggacgcacg tccgcacatc gtcggcaagt acgacgagga
      961 cggcaactgg atcctgccat cgaccggcga gctgaccccg gcgatgatcg cgctggttat
     1021 cgccaagcgc ctgtcgcgct tcttcaccag cgaagcgatg gccgcacgcg tggcctggat
     1081 cgacgccaag gaacacgagc tgaccctgcc gcgcgcgaat ttcccgcgcg tgccgcatta
     1141 ctgctcg
//
LOCUS       DASRVO010000879         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_155466, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000879 DASRVO010000000
VERSION     DASRVO010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155466"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFF05_10935"
     CDS             <1..493
                     /locus_tag="VFF05_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZW52368.1"
                     /translation="DILAQREMWFTRGPLFDAIRASIAIPTVFRPHHYEGRVLVDGGL
                     LNPVPITPTLRDLTDCTIAVDLNAAPDDDDSTPMPDVNTDPGLLETFARSLDAVQATL
                     TRFKLAAQPPDLVVSIPRNAGAFYEFHRAPELIELGRTRTRAALARWRGHVISVEGAP
                     EVS"
     gene            complement(462..689)
                     /locus_tag="VFF05_10940"
     CDS             complement(462..689)
                     /locus_tag="VFF05_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52369.1"
                     /translation="MRACTTRALTPSATLAPSALTPPYWISMGAMAISALADVFLLDY
                     GAGVTFVGRIVADAPELVRPTFRKPPAHLPR"
     gene            complement(846..>1812)
                     /gene="rho"
                     /locus_tag="VFF05_10945"
     CDS             complement(846..>1812)
                     /gene="rho"
                     /locus_tag="VFF05_10945"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019183508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZW52370.1"
                     /translation="AGRVRHPKEGERYFALLRVDTINGEPPEASKNKVLFENLTPLFP
                     RRMFHLERGNGSTEDITGRILDLIAPVGCGQRGMIVSQPKAGKTMMLQNVAQALVHNY
                     PDVHLIILLIDERPEEVTEMQRSVRAEVISSTFDEPAVRHVQVAEMVIERAKRLVEHK
                     KDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNVEEGGSLTI
                     LATALVETGSKMDEVIYEEFKGTGNMEVHLDRRISEKRVYPAININRSGTRREELLIE
                     PDMLQKIWILRKLLHPMDELAAMEFMLDKMKNTKSNDEFFNAMKR"
BASE COUNT          292 a          594 c          583 g          343 t
ORIGIN      
        1 cgacatcctc gcccaacgcg aaatgtggtt cacgcgcggt ccgctgttcg acgcaatccg
       61 cgcctcgatc gctattccca ccgtgttccg gccgcaccat tacgagggcc gcgtgctggt
      121 cgacggcggc ctgctcaatc cggtgccgat cacgccgacc ctgcgcgacc tgaccgactg
      181 cacgatcgcg gtggacctca acgccgcgcc ggacgacgac gattccacgc cgatgccgga
      241 cgtgaacacc gatccaggcc tgctcgaaac tttcgcgcgc tcgctcgacg cggtgcaagc
      301 cacgctcacg cgcttcaagc tcgccgcgca gccgccggac ctggtcgtgt cgattccgcg
      361 caatgcgggc gcgttctacg agttccaccg tgcgccggag ctgatcgaac tgggccgcac
      421 gcgcacgcgc gcggcgctcg cgcggtggcg cgggcatgtc atcagcgtgg aaggtgcgcc
      481 ggaggtttcc tgaacgtcgg gcgcacgagt tccggcgcat ccgcgacgat cctgccgacg
      541 aaggtgacgc cggcaccgta gtcgagcagg aacacgtcgg cgagggccga aatcgccatc
      601 gcgcccatgc tgatccagta cggcggggtc agcgccgacg gtgcgagtgt ggcggatggc
      661 gtcaaggcac gtgtcgtgca tgcgcgcatg gttcgccgat cgccgcgcgc ggcgaatgcg
      721 tgtaccagat ctacgcgcgg ctgccggaaa tcggactgct cacggtgttc ctgccgaacc
      781 tgcgccagcg cgcggcgtat tgattacgcc acgaaaaagg ccggcttgcg ccggccttgg
      841 tcttcttacc gcttcatcgc gttgaagaac tcgtcgttcg acttggtgtt cttcatcttg
      901 tcgagcatga attccatcgc ggcgagctcg tccatcggat gcagcagttt gcgcaggatc
      961 cagatcttct gcagcatgtc cggctcgatc agcagttctt cgcggcgcgt gccggaacgg
     1021 ttgatgttga tggcggggta gacgcgcttt tcggaaatac ggcgatccag gtgcacttcc
     1081 atgttgccgg tgcccttgaa ttcctcgtag atcacctcgt ccatcttcga gccggtctcg
     1141 accagcgcgg tggcgaggat ggtcagtgaa ccgccttcct cgacgttgcg cgcggcgccg
     1201 aagaaacgct tcgggcgctg cagcgcgttc gcgtccacgc cgccggtgag caccttgccg
     1261 gaggccggca ccacggtgtt gtaggcgcgc gccaggcgcg tgatcgagtc gagcaggatg
     1321 accacgtcct tcttgtgctc gaccaggcgc ttggcgcgct cgatcaccat ttcggcgacc
     1381 tgcacgtggc gcacggccgg ttcgtcgaac gtggacgaga tcacctcggc gcgcacgctg
     1441 cgctgcattt cggtgacttc ttccgggcgc tcgtcgatga gcaggatgat caggtgcacg
     1501 tcgggatagt tgtgcaccag cgcctgggcc acgttttgca gcatcatggt cttgccggcc
     1561 ttgggctgcg acacgatcat gccgcgctgg ccgcaaccga ccggcgcgat caaatcgaga
     1621 atgcgtccgg tgatgtcttc ggtcgaaccg ttgccacgct ccagatggaa catcctgcgc
     1681 ggaaacagcg gcgtcaggtt ctcgaacaag accttgttct tggacgcttc cggcggttcg
     1741 ccattgatcg tgtccacgcg cagcaacgca aaataacgct cgccttcctt cgggtgacgc
     1801 acgcgaccgg cg
//
LOCUS       DASRVO010000880         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_363188, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000880 DASRVO010000000
VERSION     DASRVO010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363188"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /gene="fusA"
                     /locus_tag="VFF05_10950"
     CDS             complement(<1..1603)
                     /gene="fusA"
                     /locus_tag="VFF05_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZW52371.1"
                     /translation="MAYTTDGIRNIALAGHSAAGKTTLFEALLHAGGVIGAAGSVERG
                     TTVSDYDPMEKERKQSLNTAIASIDTGDCHINVIDTPGAADFRGPTLSALAAVETCAV
                     VVNAHNGIEYGTQRMMHHAKARGLARMLIVNKMDGGNCRALVDQLREAFGVECLPINL
                     PAQDGKSVVDCFFKPEGVADFSSVAEAHQRIIDQVVEINETVMNRFLDVGEAGLSGQE
                     LHDAFEQCLREGHLVPICFVSARTGAGVKELLNLIARLLPNPTEGNPPPFRKGAGANA
                     APIEAVPDPKQHVIADVFKIVNDPFVGKLSVFRVYQGTVKKDTQLFIDDGKKPFKVGH
                     LFKLRGKEHIEIDSAIPGDIAAVAKVEEIHFDAVLHDSHDEDHIHLKPLDFPQPMFGL
                     AVEPAHKGQEQKLATSLHKLSEEDPCFRFEHNKELNESVVRGLSDLHLKLMLERMKQR
                     YDVEVKARPPKIAYRETVAAKAEGHHRHKKQTGGAGQFGEVYLRVEPLPRGAGFEFAD
                     EVKGGTIPNQFIPAVEKGVRQVLEHG"
     gene            1751..>2103
                     /locus_tag="VFF05_10955"
     CDS             1751..>2103
                     /locus_tag="VFF05_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52372.1"
                     /translation="MTQKEVFARGDATPEQAGWDAEVRERALARLSRRAQRRPATGWL
                     LALIALAGLHALFFWHWLQNRFSPVVVAAGMVMEVRLVDEPAPPRAPEAVAPSSRTPA
                     HMAAAMLPAVPSPVSA"
BASE COUNT          321 a          672 c          677 g          433 t
ORIGIN      
        1 cgccgtgttc gagaacctgg cgcacgcctt tttccacggc cggaatgaac tggttcggga
       61 tcgtgccgcc cttcacctcg tcggcgaact cgaaccccgc gccgcgcggc agcggctcga
      121 cgcgcaggta gacttcgccg aactggccgg cgccgccggt ctgtttcttg tggcggtgat
      181 gcccttccgc cttcgccgcc accgtttcgc gataggcgat cttcggcggc cgcgccttca
      241 cctccacgtc gtagcgctgc ttcatgcgtt cgagcatcag tttcaggtgc aggtcggaca
      301 aaccgcgcac gacggattcg ttcaattcct tgttgtgctc gaaacgaaag cacggatcct
      361 cctcgctcaa cttgtgcagc gatgtggcaa gtttttgttc ctggcccttg tgcgccggtt
      421 ccaccgcgag gccgaacatc ggctgcggaa agtcgagcgg cttgaggtgg atgtgatcct
      481 cgtcgtgcga atcgtgcagc accgcgtcga aatggatttc ctccactttg gccaccgccg
      541 cgatgtcacc gggaatcgcg ctgtcgatct cgatgtgttc cttgccgcgg agcttgaaca
      601 ggtgtccgac cttgaaaggt ttcttgccgt cgtcgatgaa gagctgcgtg tccttcttca
      661 ccgtgccctg gtagacacgg aacacgctga gcttgccgac aaagggatca ttgacgatct
      721 tgaacacgtc ggcgatgaca tgctgtttgg gatcgggcac ggcttcgatc ggcgccgcgt
      781 tcgcgccggc gcccttgcgg aacggcggcg ggttgccttc ggtcggattg ggcagcagcc
      841 ttgcgatgag gttcagcagt tccttcacgc ccgctccggt gcgcgcggat acgaagcaga
      901 tcggcaccag atggccctcg cgcaggcatt gttcgaacgc atcgtgcaat tcctggccgg
      961 aaagtcccgc ttccccgacg tccaggaaac ggttcatcac ggtttcattg atctcgacga
     1021 cctggtcgat gatgcgctgg tgcgcttcgg cgacggaaga aaaatccgcc acgccttccg
     1081 gtttgaagaa acaatcgacc acggatttgc cgtcctgcgc gggcaagttg atcggcaggc
     1141 attccacgcc gaacgcttcg cgcaactggt cgaccagggc gcgacagttg ccgccgtcca
     1201 tcttgttgac gatcagcatg cgcgccaggc cgcgcgcctt ggcgtggtgc atcatgcgct
     1261 gcgtgccgta ttcgatgccg ttgtgcgcgt tgacgacgac ggcgcaggtt tccacggcgg
     1321 ccagcgcgct cagcgtcggc ccgcggaaat ccgccgcgcc gggcgtgtcg atcacgttga
     1381 tgtggcagtc gccggtgtcg atggaggcga ttgccgtatt cagggactgc ttgcgttcct
     1441 tttccatcgg gtcgtagtcg gaaaccgtag tgccgcgctc gaccgaaccc gctgcgccga
     1501 tcacgccgcc cgcgtgcagc agggcttcga acagcgtggt cttgcccgcg gcggaatgtc
     1561 cggcgagcgc gatgtttcgg atgccatcgg tggtgtaggc catgagcgtc tcccgtgtgc
     1621 gtggcgtgga cctgcaagcg ttctcctttc gggacgctgc gtcaaggcgc gatgcgacag
     1681 cgaaagatta acccccgatt tacagaccga ttgctaggct cgccgcgctc gaactggacg
     1741 cgggatcgca gtgacgcaaa aggaagtttt cgctcgcggc gacgccacgc cggagcaggc
     1801 cggatgggac gccgaagtcc gcgagcgtgc gcttgcgcgc ctgtcgcgcc gtgcgcaacg
     1861 ccggcccgcc acgggttggc tgcttgcgct catcgcgttg gcgggccttc acgcactgtt
     1921 tttctggcac tggctgcaga accggttttc gcctgtggtt gtggctgccg gcatggtcat
     1981 ggaagtgcgc ctggtcgacg aacccgcgcc gccgcgcgca cccgaagccg tggcgccgtc
     2041 gtcccgcacc cctgcgcaca tggctgcggc catgttgccc gccgtgccat cgcctgtgtc
     2101 ggc
//
LOCUS       DASRVO010000881         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_363472, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000881 DASRVO010000000
VERSION     DASRVO010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363472"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /gene="mutS"
                     /locus_tag="VFF05_10960"
     CDS             complement(<1..1587)
                     /gene="mutS"
                     /locus_tag="VFF05_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZW52373.1"
                     /translation="MPSPDLAQHTPLMRQYFAAKAEYPDTLVFFRMGDFYELFYDDAR
                     RAARLLDLTLTQRGASAGAPIPMAGVPYHAAEGYLARLIKLGESVAICEQIGDPALAK
                     GLVERRVVRVVTPGTVTDEALLEERRDNLLLAIAAGKTGFGLAWIDLAGGRFLLSEVA
                     DAEALASELARLAPAETLIPEDAVWPEFVAALPGLRKRAPWHFDAETAQRRLCAFFGV
                     RDLSGFGCDGLPLAIAAAGALLGYVEETQKSALPHLSGLAVENAGETIALDAATRRNL
                     ELDTHPSGNIENTLLGVLDETVTPMGARELRRRLHRPLRNRDLLRRRYQAIETLIDAR
                     RFENLREVLRSIGDLERILARVALRSARPRDLSTLRDGLLAAPELRRALHECDSPLIA
                     ELLERIGEHANTAQHLAAAVLEQPPALLRDGGVIRPGFDAELDELRTLSTNADQFLVD
                     LEAREKAATGIPTLKVGYNSVHGYYIEISKGQSDKAPTHYTRRQTLKGAERYITEELK
                     AFEDKVLSARERALMRERALY"
BASE COUNT          295 a          542 c          580 g          311 t
ORIGIN      
        1 gtacagcgcg cgttcgcgca tcaatgcacg ttcgcgcgcg gacagcacct tgtcctcgaa
       61 tgcctttaac tcctcggtga tgtagcgctc ggcgcccttc agggtctgcc gccgcgtgta
      121 gtgcgtcggc gccttgtcgg attggccctt gctgatttcg atgtagtagc cgtgcacgct
      181 gttgtagccg accttcagag tcggaatgcc ggtcgcggct ttttcacgcg cttccagatc
      241 gaccaggaat tgatcggcat tcgtcgacag cgtgcgcagt tcgtcgagtt cggcgtcgaa
      301 tccggggcgg atcacgccgc cgtcgcgcag cagcgcgggc ggttgttcga gcacggcggc
      361 ggcgaggtgt tgcgcggtgt tggcgtgttc gccgatgcgt tcaagcaatt ccgcgatcaa
      421 tggcgagtcg cattcatgca atgcccgccg caactcgggt gccgccagca atccatcgcg
      481 caaggtcgac aggtcgcgtg ggcgcgcgct gcgcagggcc acgcgcgcga gtatgcgttc
      541 caggtcgccg atgctgcgca gcacttcgcg aagattctcg aaacggcgag catcgatcag
      601 ggtttcaatg gcctgatagc ggcgtcgcag cagatcgcga ttgcgtagag ggcgatgcag
      661 ccggcggcgc aattcgcgcg cgcccatcgg cgtcaccgtt tcgtcgagca cgccgagcaa
      721 ggtgttttcg atgttgcccg atggatgcgt atccagttcc agattgcggc gcgtggccgc
      781 atccagcgcg atcgtttcgc cggcgttttc cacggcaaga ccggaaagat gcggcaacgc
      841 ggatttctgc gtctcctcga catagccgag caaggcgccg gccgcggcga tcgccagcgg
      901 caggccatcg cagccgaagc cggacaggtc gcgcacgccg aagaacgcgc acaatcggcg
      961 ctgcgcggtc tcggcgtcga aatgccaggg cgcgcgtttg cgcaggccgg gcagggcagc
     1021 gacgaattcg ggccacaccg cgtcctcggg gatcagcgtt tctgccggcg caaggcgcgc
     1081 gagttcggat gccagcgctt cggcatcggc gacttcgctc aatagaaaac gcccgccggc
     1141 caggtcgatc caggccagtc cgaatcctgt tttgccagcg gcaatggcga gcaacagatt
     1201 gtctcggcgc tcttcgagca gggcctcgtc ggtgacggtg cccggcgtga ccacgcgcac
     1261 gacacggcgt tcgaccaggc ccttggccag cgccggatcg ccgatctgtt cgcagatcgc
     1321 caccgactcg ccgagtttta tcaatcgcgc gagatagcct tcggcggcgt gatagggcac
     1381 gcccgccatc ggaatcggcg cgcccgccga cgcaccgcgc tgggtcagtg tgaggtcgag
     1441 cagccgcgcc gcgcggcgcg cgtcgtcgta gaacaattcg tagaaatcgc ccatacggaa
     1501 gaacaccagc gtgtccggat actccgcctt ggcggcaaag tattggcgca tcaggggtgt
     1561 gtgctgggcg aggtcgggcg agggcatccg gcggggttcg cgaaacgggc ggctagttta
     1621 ccctgacgat cgagatcgcc gggacgcctg tcatccaacc gtaaccgaat ccggttgtaa
     1681 tcgccgcgcc gcgaactttg cgataggctg tcacaatgca aaaacgta
//
LOCUS       DASRVO010000882         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rudaea sp. isolate SMAG_U9451 k141_363605, whole
            genome shotgun sequence.
ACCESSION   DASRVO010000882 DASRVO010000000
VERSION     DASRVO010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970208
            Sequence Read Archive: SRR5259839
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rudaea sp. (soil metagenome)
  ORGANISM  Rudaea sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Rhodanobacteraceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 00:05:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,194
            CDSs (total)                      :: 2,175
            Genes (coding)                    :: 2,160
            CDSs (with protein)               :: 2,160
            Genes (RNA)                       :: 19
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 5 of 15
            Pseudo Genes (incomplete)         :: 9 of 15
            Pseudo Genes (internal stop)      :: 3 of 15
            Pseudo Genes (multiple problems)  :: 2 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Rudaea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363605"
                     /isolate="SMAG_U9451"
                     /isolation_source="soil"
                     /db_xref="taxon:2136325"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="38.1087 N 121.653 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFF05_10965"
     CDS             <1..838
                     /locus_tag="VFF05_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZW52374.1"
                     /translation="YLPAASARAAAPSENVRKSPMPVAAQASKHKSPAHDPKAARILD
                     VEGVGAVSVYAPQGTSKGMALFASGDGGWNLGVWDMAQTAASLGYWVAGFSTPALLKS
                     LDSGAGACSDAAAAAEKIGNEVKSAMNLPQDFRPLLIGYSSGATVVYAALAQAGDTRF
                     SGAMTLGFCPDLIYHKPFCEDAGLTADKQHKPPYGFVFNTVPRVPAPWIVLQGDIDKV
                     CSPPATVSFVEKVKNGKVVSLPHVGHGYGVPRNWMPQYRAGLIELLRAAAPEPRVAER
                     NR"
     gene            855..>2342
                     /locus_tag="VFF05_10970"
     CDS             855..>2342
                     /locus_tag="VFF05_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HZW52375.1"
                     /translation="MSEESAAASSVIARLRWIVPVAFLALTAWLLWREAGTFPLEDIQ
                     HTLIDVPTLPALGVAALALVGVTFTGSVDWLIARWLKLGLHTRDCFRLAFVANSMANT
                     LNLSGAMGASVRLMGLSGLKVPLSRAAALIGMQALSLLSGLSLLVIATLATSSLPLTS
                     GSAQRWIAAAVLIAAALYLPLYFFLTTRRALMRWLPSDVGIPPLRLKIELTLVSLIDW
                     LLAAATLYACIYLSGEHVKPGLLLGAFAGASVLGLVSQVPAGIGVVDGLILLALTEAG
                     YDKAAVVSGLMLFRIAYYLLPMLIALYLGSEMLTARLPLLARLRTRLAGHPLFGLLGL
                     PATLLADFGTRLLAILTFGAGAMQLASAAIPSVSEQIETVRAHLPILAVESSNWFSVV
                     SGVLLLGLARGIDGRLRIAYRVAMWLLLISAALAVTKGLHFGEALFLLAVAALLRARK
                     RVFSRRAMTLTSARTFGWFAGLLICVLVFFAIGVAQVLGDDSFDLF"
BASE COUNT          321 a          794 c          782 g          445 t
ORIGIN      
        1 ttacctgccc gcggcatccg cgcgcgccgc cgcgccatcg gaaaacgtgc gcaaatctcc
       61 aatgccggtg gccgcgcagg cgtcgaaaca caaatcgccc gcccacgatc ccaaggccgc
      121 gcgcattctc gatgtcgaag gcgtcggcgc ggtgagcgta tacgcgccgc aaggcacgtc
      181 caagggcatg gccctgttcg cctccggcga cggcggctgg aatctgggcg tgtgggacat
      241 ggcacagacg gcggcgagcc tggggtactg ggtggcggga ttcagtaccc cggcgctgct
      301 caaatcgctg gattccggcg cgggcgcctg cagcgatgcc gccgccgccg cggaaaagat
      361 cggcaacgaa gtgaagtccg cgatgaacct gccgcaggat tttcggccgt tgctgatcgg
      421 ttactcgtcc ggcgccacgg tcgtctacgc ggcgctcgcg caggccgggg acacgcgctt
      481 ttccggcgcg atgacgctcg gtttctgccc cgacctcatc taccacaagc cgttctgcga
      541 ggacgcggga cttaccgcgg acaagcagca caagccacct tacggcttcg tgttcaacac
      601 cgtgccgcgc gtaccggcgc catggatcgt gctgcagggc gacatcgaca aggtctgcag
      661 cccgccggcg acggtaagct tcgtggaaaa agtgaagaat ggaaaagtgg tcagcctgcc
      721 gcatgtcggc catggctacg gcgtgccgcg caactggatg ccgcagtacc gcgccggcct
      781 gatcgaactg ctgcgcgccg ccgcaccgga accgcgcgtg gccgagcgca accgctgaag
      841 cccggccacg cccgatgagc gaggaatccg ccgccgcgtc cagcgtcatc gcccgcctgc
      901 gctggatcgt gccggtcgct ttcctggcgc tcaccgcctg gctgctgtgg cgcgaggccg
      961 gcacgtttcc gctcgaagac atccagcaca ccctgatcga cgtgccgacc ctgcccgcgc
     1021 tcggcgtggc cgcgctcgcg ctggtcggcg tcaccttcac cggcagcgtg gactggctga
     1081 tcgcgcgctg gctcaagctc ggcctgcaca cgcgcgattg cttccgcctc gccttcgtcg
     1141 ccaactccat ggcgaacacg ctcaatcttt ccggcgcgat gggcgcgagc gtgcgcctga
     1201 tgggcttgag cggattgaaa gtgccgttgt cgcgcgcggc ggcgctcatc ggcatgcagg
     1261 cgttgtcgct gctctcgggt ttgtcgttgc tcgtgatcgc cacgctggcg acgagttcct
     1321 tgccgctgac cagcggcagc gcgcagcgct ggatcgccgc agccgtgctg atcgcagcgg
     1381 cgttgtatct gccgttgtac ttcttcctca ccacgcgccg cgcactgatg cgctggctgc
     1441 cgtccgatgt cggcatcccg ccgctgcggc tgaagatcga actcacgctg gtctcgctga
     1501 tcgactggct gctcgccgcg gcgacgctgt acgcctgcat ttacctttca ggcgaacacg
     1561 tcaagccggg cctgttgctc ggcgcgttcg ccggtgcgtc cgtgctgggt ttggtcagcc
     1621 aagtgccggc gggtattggt gtcgtcgacg gcctgatcct gctcgcactg accgaggccg
     1681 gttacgacaa ggccgcggtg gtgtcggggc tgatgctgtt ccgcatcgcc tattacctgc
     1741 tgccgatgct cattgcgctg tacctgggct cggaaatgct gaccgcgcgc ctgccgctgt
     1801 tggcacgact gcgcacgcgc ctcgccgggc atccgctgtt cggcctgctc ggcctgccgg
     1861 cgacgttgct cgccgatttc ggcacacgcc tgctcgcgat cctgaccttc ggcgccggtg
     1921 cgatgcagtt ggcatcggca gcgattccgt cggtgagcga acagatcgaa accgtgcgcg
     1981 cgcacctgcc gatcctcgcg gtggaaagct cgaactggtt ttcggtggtc tcgggcgtgc
     2041 tgttgctggg cttggcgcgc ggcatcgacg gccgcctgcg catcgcctat cgcgtggcga
     2101 tgtggctgct gctgatcagc gcggcgctgg ccgtgaccaa gggcctgcat ttcggcgaag
     2161 cgttgttcct gctcgcggtc gccgcgctgc tgcgcgcgcg caagcgcgtg ttctcgcggc
     2221 gtgcgatgac actgacctcg gcacgaacct tcggctggtt cgcgggccta ttgatttgtg
     2281 tgctggtgtt ctttgccatc ggcgttgccc aggtgctggg cgacgattcc ttcgacctgt
     2341 tc
//
